LOCUS       DAHHPA010000001        79824 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.001, whole genome shotgun sequence.
ACCESSION   DAHHPA010000001 DAHHPA010000000
VERSION     DAHHPA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.001"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            65..1021
                     /locus_tag="NBH55_000001"
     CDS             65..1021
                     /locus_tag="NBH55_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCD1508023.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1094..1210
                     /locus_tag="NBH55_000002"
     CDS             1094..1210
                     /locus_tag="NBH55_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCD1508024.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1265..2947)
                     /gene="recN"
                     /locus_tag="NBH55_000003"
     CDS             complement(1265..2947)
                     /gene="recN"
                     /locus_tag="NBH55_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCD1508025.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2965..3414)
                     /locus_tag="NBH55_000004"
     CDS             complement(2965..3414)
                     /locus_tag="NBH55_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCD1508026.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3521..4339)
                     /locus_tag="NBH55_000005"
     CDS             complement(3521..4339)
                     /locus_tag="NBH55_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCD1508027.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4348..5238)
                     /locus_tag="NBH55_000006"
     CDS             complement(4348..5238)
                     /locus_tag="NBH55_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD1508028.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5240..5464)
                     /locus_tag="NBH55_000007"
     CDS             complement(5240..5464)
                     /locus_tag="NBH55_000007"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCD1508029.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5469..6806)
                     /locus_tag="NBH55_000008"
     CDS             complement(5469..6806)
                     /locus_tag="NBH55_000008"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCD1508030.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6807..7661)
                     /locus_tag="NBH55_000009"
     CDS             complement(6807..7661)
                     /locus_tag="NBH55_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCD1508031.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7758..8207)
                     /gene="nusB"
                     /locus_tag="NBH55_000010"
     CDS             complement(7758..8207)
                     /gene="nusB"
                     /locus_tag="NBH55_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCD1508032.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8200..8625)
                     /locus_tag="NBH55_000011"
     CDS             complement(8200..8625)
                     /locus_tag="NBH55_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1508033.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8871..9935)
                     /locus_tag="NBH55_000012"
     CDS             complement(8871..9935)
                     /locus_tag="NBH55_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD1508034.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10107..10352)
                     /locus_tag="NBH55_000013"
     CDS             complement(10107..10352)
                     /locus_tag="NBH55_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD1508035.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10491..10865)
                     /locus_tag="NBH55_000014"
     CDS             complement(10491..10865)
                     /locus_tag="NBH55_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCD1508036.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10948..11862)
                     /locus_tag="NBH55_000015"
     CDS             complement(10948..11862)
                     /locus_tag="NBH55_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCD1508037.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFSPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11883..12686)
                     /locus_tag="NBH55_000016"
     CDS             complement(11883..12686)
                     /locus_tag="NBH55_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCD1508038.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(12719..13708)
                     /gene="manX"
                     /locus_tag="NBH55_000017"
     CDS             complement(12719..13708)
                     /gene="manX"
                     /locus_tag="NBH55_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCD1508039.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(13811..14302)
                     /locus_tag="NBH55_000018"
     CDS             complement(13811..14302)
                     /locus_tag="NBH55_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1508040.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14493..17300)
                     /locus_tag="NBH55_000019"
     CDS             complement(14493..17300)
                     /locus_tag="NBH55_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCD1508041.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(17734..18027)
                     /gene="rpmA"
                     /locus_tag="NBH55_000020"
     CDS             complement(17734..18027)
                     /gene="rpmA"
                     /locus_tag="NBH55_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCD1508042.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18040..18384)
                     /locus_tag="NBH55_000021"
     CDS             complement(18040..18384)
                     /locus_tag="NBH55_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCD1508043.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18411..18719)
                     /gene="rplU"
                     /locus_tag="NBH55_000022"
     CDS             complement(18411..18719)
                     /gene="rplU"
                     /locus_tag="NBH55_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCD1508044.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(18739..18815)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19001..19642
                     /locus_tag="NBH55_000023"
     CDS             19001..19642
                     /locus_tag="NBH55_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCD1508045.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(19704..21335)
                     /locus_tag="NBH55_000024"
     CDS             complement(19704..21335)
                     /locus_tag="NBH55_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCD1508046.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(21458..21817)
                     /locus_tag="NBH55_000025"
     CDS             complement(21458..21817)
                     /locus_tag="NBH55_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCD1508047.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(21976..22049)
                     /locus_tag="NBH55_000026"
     tRNA            complement(21976..22049)
                     /locus_tag="NBH55_000026"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22013..22015),aa:Arg,seq:tct)
     gene            complement(22358..23041)
                     /locus_tag="NBH55_000027"
     CDS             complement(22358..23041)
                     /locus_tag="NBH55_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCD1508048.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(23169..23399)
                     /locus_tag="NBH55_000028"
                     /pseudo
     CDS             complement(23169..23399)
                     /locus_tag="NBH55_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
     gene            complement(23577..24770)
                     /locus_tag="NBH55_000029"
     CDS             complement(23577..24770)
                     /locus_tag="NBH55_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1508049.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(24854..25054)
                     /locus_tag="NBH55_000030"
     CDS             complement(24854..25054)
                     /locus_tag="NBH55_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCD1508050.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(25478..25711)
                     /locus_tag="NBH55_000031"
     CDS             complement(25478..25711)
                     /locus_tag="NBH55_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508051.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(25718..26149)
                     /locus_tag="NBH55_000032"
     CDS             complement(25718..26149)
                     /locus_tag="NBH55_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD1508052.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            26497..26670
                     /locus_tag="NBH55_000033"
     CDS             26497..26670
                     /locus_tag="NBH55_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508053.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            complement(26736..27344)
                     /locus_tag="NBH55_000034"
     CDS             complement(26736..27344)
                     /locus_tag="NBH55_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HCD1508054.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(27350..28378)
                     /gene="vanB"
                     /locus_tag="NBH55_000035"
     CDS             complement(27350..28378)
                     /gene="vanB"
                     /locus_tag="NBH55_000035"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HCD1508055.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            complement(28371..29342)
                     /locus_tag="NBH55_000036"
     CDS             complement(28371..29342)
                     /locus_tag="NBH55_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HCD1508056.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(29339..30166)
                     /gene="vanW"
                     /locus_tag="NBH55_000037"
     CDS             complement(29339..30166)
                     /gene="vanW"
                     /locus_tag="NBH55_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HCD1508057.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            complement(30184..30990)
                     /gene="vanY"
                     /locus_tag="NBH55_000038"
     CDS             complement(30184..30990)
                     /gene="vanY"
                     /locus_tag="NBH55_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCD1508058.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            complement(31161..32504)
                     /gene="vanS"
                     /locus_tag="NBH55_000039"
     CDS             complement(31161..32504)
                     /gene="vanS"
                     /locus_tag="NBH55_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCD1508059.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            complement(32507..33166)
                     /gene="vanR"
                     /locus_tag="NBH55_000040"
     CDS             complement(32507..33166)
                     /gene="vanR"
                     /locus_tag="NBH55_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HCD1508060.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(33784..33951)
                     /locus_tag="NBH55_000041"
     CDS             complement(33784..33951)
                     /locus_tag="NBH55_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508061.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            complement(34109..34294)
                     /locus_tag="NBH55_000042"
     CDS             complement(34109..34294)
                     /locus_tag="NBH55_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1508062.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            34439..34810
                     /locus_tag="NBH55_000043"
     CDS             34439..34810
                     /locus_tag="NBH55_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1508063.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            35068..35397
                     /gene="mobC"
                     /locus_tag="NBH55_000044"
     CDS             35068..35397
                     /gene="mobC"
                     /locus_tag="NBH55_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCD1508064.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            35358..36686
                     /locus_tag="NBH55_000045"
     CDS             35358..36686
                     /locus_tag="NBH55_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCD1508065.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            36668..37054
                     /locus_tag="NBH55_000046"
     CDS             36668..37054
                     /locus_tag="NBH55_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HCD1508066.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            37237..37473
                     /locus_tag="NBH55_000047"
     CDS             37237..37473
                     /locus_tag="NBH55_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508067.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(37773..37976)
                     /locus_tag="NBH55_000048"
     CDS             complement(37773..37976)
                     /locus_tag="NBH55_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HCD1508068.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(37980..38924)
                     /locus_tag="NBH55_000049"
     CDS             complement(37980..38924)
                     /locus_tag="NBH55_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508069.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(38925..42845)
                     /locus_tag="NBH55_000050"
     CDS             complement(38925..42845)
                     /locus_tag="NBH55_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HCD1508070.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(42994..45075)
                     /locus_tag="NBH55_000051"
     CDS             complement(42994..45075)
                     /locus_tag="NBH55_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCD1508071.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFANVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(45072..46301)
                     /locus_tag="NBH55_000052"
     CDS             complement(45072..46301)
                     /locus_tag="NBH55_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HCD1508072.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(46291..46542)
                     /locus_tag="NBH55_000053"
     CDS             complement(46291..46542)
                     /locus_tag="NBH55_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HCD1508073.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(46565..48556)
                     /locus_tag="NBH55_000054"
     CDS             complement(46565..48556)
                     /locus_tag="NBH55_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCD1508074.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(48588..50987)
                     /locus_tag="NBH55_000055"
     CDS             complement(48588..50987)
                     /locus_tag="NBH55_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCD1508075.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(50917..51339)
                     /locus_tag="NBH55_000056"
     CDS             complement(50917..51339)
                     /locus_tag="NBH55_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCD1508076.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(51353..51889)
                     /locus_tag="NBH55_000057"
     CDS             complement(51353..51889)
                     /locus_tag="NBH55_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HCD1508077.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(51926..52789)
                     /locus_tag="NBH55_000058"
     CDS             complement(51926..52789)
                     /locus_tag="NBH55_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508078.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(52881..53012)
                     /locus_tag="NBH55_000059"
     CDS             complement(52881..53012)
                     /locus_tag="NBH55_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508079.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(53037..54734)
                     /locus_tag="NBH55_000060"
     CDS             complement(53037..54734)
                     /locus_tag="NBH55_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1508080.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            complement(54761..54943)
                     /locus_tag="NBH55_000061"
     CDS             complement(54761..54943)
                     /locus_tag="NBH55_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508081.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            complement(55628..57544)
                     /locus_tag="NBH55_000062"
     CDS             complement(55628..57544)
                     /locus_tag="NBH55_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1508082.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            complement(57574..59301)
                     /locus_tag="NBH55_000063"
     CDS             complement(57574..59301)
                     /locus_tag="NBH55_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCD1508083.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            complement(59301..59666)
                     /locus_tag="NBH55_000064"
     CDS             complement(59301..59666)
                     /locus_tag="NBH55_000064"
                     /inference="COORDINATES: protein motif:HMM:NF024360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HCD1508084.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            complement(59867..60097)
                     /locus_tag="NBH55_000065"
     CDS             complement(59867..60097)
                     /locus_tag="NBH55_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508085.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            complement(60085..60651)
                     /locus_tag="NBH55_000066"
     CDS             complement(60085..60651)
                     /locus_tag="NBH55_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HCD1508086.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            complement(60644..60868)
                     /locus_tag="NBH55_000067"
     CDS             complement(60644..60868)
                     /locus_tag="NBH55_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HCD1508087.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            complement(61191..62702)
                     /locus_tag="NBH55_000068"
     CDS             complement(61191..62702)
                     /locus_tag="NBH55_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508088.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMGV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            complement(62815..62997)
                     /locus_tag="NBH55_000069"
     CDS             complement(62815..62997)
                     /locus_tag="NBH55_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HCD1508089.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            complement(63031..63261)
                     /locus_tag="NBH55_000070"
     CDS             complement(63031..63261)
                     /locus_tag="NBH55_000070"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HCD1508090.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            complement(63440..63865)
                     /locus_tag="NBH55_000071"
     CDS             complement(63440..63865)
                     /locus_tag="NBH55_000071"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1508091.1"
                     /translation="MIRELRKVDINKVAEIWLDTNIKTHYFISAQYWKSNFELVKELL
                     LQATVYVYEDKQEIQGFIGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNV
                     YQKNTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            complement(63923..64444)
                     /locus_tag="NBH55_000072"
     CDS             complement(63923..64444)
                     /locus_tag="NBH55_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1508092.1"
                     /translation="MIRKAILKDVNSIEDTYNEHFQYELNHTAFTVFKKGVYPTKDDA
                     ERAIYAGALFVYEENGTIVGSIIIDKVQPIEYATIPWKEKLSEDEVMVIHLLMVRPSM
                     SGKGIASSLIKFATELAQKNSCRALRLDTGSQNIPALSLYQKNGFEIIASASKKVGDV
                     IAHKNHLFLEKIL"
     gene            complement(64791..64997)
                     /locus_tag="NBH55_000073"
     CDS             complement(64791..64997)
                     /locus_tag="NBH55_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1508093.1"
                     /translation="MGVSYNRLWKMLIDKNMKRIEMQYLTGISGNILARMGKNQYVSM
                     ETIEKICKKLDCTVDEMLEFTDDE"
     gene            complement(65144..65554)
                     /locus_tag="NBH55_000074"
     CDS             complement(65144..65554)
                     /locus_tag="NBH55_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1508094.1"
                     /translation="MNKVVLMGRLTKNPEIKYAGKDNDMAVARYTLAVNRRYKRDGEQ
                     EADFISCVTFGKSAEFAQKYLHKGMRIVIGGRISTGNYKDKDGKTIYTTDVIVEEHEF
                     AQNKDNGVGADSSETPKTDKDGFMEAPEGEVPFD"
     gene            complement(65578..65898)
                     /locus_tag="NBH55_000075"
     CDS             complement(65578..65898)
                     /locus_tag="NBH55_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508095.1"
                     /translation="MPGKEQFMTELKMKEMQLINWFGCPNLIYTRERIHLVAMLTTDE
                     EMKKNLYRVCRFLAREEIAYRYPIMYEMIRKIFSPDDTNPPEACAMFMPDDETDDEEY
                     AMAA"
     gene            complement(66574..67428)
                     /locus_tag="NBH55_000076"
     CDS             complement(66574..67428)
                     /locus_tag="NBH55_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCD1508096.1"
                     /translation="MVKKKNIRKSALLLIMIMLATGITACGTEEEPLPDLSAMGAVTA
                     VSREEGSGTRAEFENLLKLPESDTGIVVDSTEKVLKKVEEDKNAVGYVAYSSATDTNG
                     KILQINGVLPSEKTIDNNSYPLCRDYYLAYNGELTDVEQDFLTYVKSKGQDIVKQYCI
                     QADSTTTFLSDKSEGKILIEGSTSMEPMVKALADDYQKQNPNAEIEVKATDSSRGITA
                     VISGECDFAMSSRELKDYEAELLETKVIGKDAIAIVINEENPLENLTIKQLTGLYNGT
                     YKNWDDLS"
     gene            complement(67430..69148)
                     /locus_tag="NBH55_000077"
     CDS             complement(67430..69148)
                     /locus_tag="NBH55_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCD1508097.1"
                     /translation="MNENVKVVFGLIGGLALFLYGMNSMSDALQKAAGERMKKILGFL
                     TRNPIMGALAGALVTAVLQSSSATTVMVIGFVSAGLMSLPQAISVIFGANIGTTMTAQ
                     LMAFKISNYIYPIIFVGFILNFVSKKEKVKNIGMVIFSFGLLFEGIEIMGEVMKPLAG
                     SPVFVDLMGKVSSIPVLGVVLGAVMTLVVQSSSATIAVLQNFASQAGPDGVSSVIGLT
                     GAIPILLGDNIGTTITALLASIGQSKNAKRTAIAHSIFNISGSCVFIFLVPWFAKFVQ
                     FISPKGNEIDVISRQIANAHTTFNIVCTLVWLPLIPLMVKIVTTIIRGNDKTEKAAFE
                     PKYLDMKVIEQPAAAMVLVSKELNRLGELAESLLSDLKTAIVADGDSKTHGSFIENLE
                     IVHQLQDSVTEYITRLFASGNLTEQQSEQTAGLLYVNNSIQRIADRCEDIDQICEKVN
                     NNGKRLTSEASSEMEDCIDIIQQLLKKAMEAIRKGDAVVAETVFKNKKKMHKAEKKYS
                     KAHLNRVKNEVCDASMTRYFSGIMYNFDRMADNCVSIAEEACDNVAFINLDEETGKSM
                     MERGAV"
     gene            complement(69321..70103)
                     /locus_tag="NBH55_000078"
     CDS             complement(69321..70103)
                     /locus_tag="NBH55_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCD1508098.1"
                     /translation="MKVKNADEFYKILERKAIYPVFQPIVNLQTGDVAGYEALSRIDR
                     HDTTLMISDLFVIAEQVGCVWKLEKLCRNKALKAAANKPEHAKLFLNVDGNIIQDKSF
                     IQGFTNRKAAKAGVPSCDIVFEITERSDIENYQILQQIMKHYADQGYEIALDDVGAGY
                     SGLNRVVNTSPNYLKVDIELVRDIQKHKKKEIMMEFLLHYCNETGAILIAEGIETEAE
                     LECLCRLGVHYGQGYFLGRPDRTFKNVRNEAMLVLRRLNNKS"
     gene            complement(70362..70643)
                     /locus_tag="NBH55_000079"
     CDS             complement(70362..70643)
                     /locus_tag="NBH55_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508099.1"
                     /translation="MRRELIESYYEEYQRELERFLESDPEMFTLEAEIHHYIEILEPV
                     LRDGGQEMWQTLDRIISAKNAMEGIVAKEMYIRGFLDYERLVCEKDSKI"
     gene            complement(70658..71038)
                     /locus_tag="NBH55_000080"
     CDS             complement(70658..71038)
                     /locus_tag="NBH55_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HCD1508100.1"
                     /translation="MTISCKAKGDTMKIEYTKVGDYYLPNLYYPEETRPIGLWGMLRK
                     EYLKEHKSGTYTYLLLTARLDSYLADLNEQAQERFELIETQMRSAEGVSEELKRQNPM
                     EWLCHCNNIRNRAAEIIKQELIYV"
     gene            complement(71079..71240)
                     /locus_tag="NBH55_000081"
     CDS             complement(71079..71240)
                     /locus_tag="NBH55_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HCD1508101.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKHLMSSM"
     gene            complement(71317..72645)
                     /locus_tag="NBH55_000082"
                     /pseudo
     CDS             complement(71317..72645)
                     /locus_tag="NBH55_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            complement(72751..74568)
                     /locus_tag="NBH55_000083"
     CDS             complement(72751..74568)
                     /locus_tag="NBH55_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCD1508102.1"
                     /translation="MRNPIHVLKSLEEKASVSNYKYERLYRNLYNPEFYLLAYANIAK
                     SQGSMTQGVDGQTLDNMSLPRINRIIESIRNRTYQPKPAKRKYIPKKNGKLRPLGITS
                     TDDKLVQEVVRMILEAIYEPTFSNNSHGFRPKRSCHTALTQVKKNFTGVTWIVEGDIK
                     ACFDNFDHHVLVELLRKRISDEAFIGLIWKFLKAGYMEQWQYNCTYSGVPQGSGISPI
                     CANIYLSELDNYMQEYKEKYDCEPERRRTTREYERASRRYRKARKALMGAEKSTPELV
                     KEFKDSRRKKMNQHYYNPFEEGFKKIQYNRYADDFVIGVIGSKKDAEKIKEDVKIFLQ
                     EKLHLEMSEEKTKVTHSSKPVRYLGYDFKVIHSKNMKRCKNGDMKRVWYGKVFLYMPK
                     EKWIKKAMERGAIQVKRNNDTGKEMWRPMPRKDLMNRSDAEIVSTFNSEIRGLYNFYR
                     IAENVGALHKYYYMVRYSMLKTLAGKHRTNVSVIKKRHMVNGVLRIPYDTTKGRKYCE
                     FYHDGFRKHSDGYDNVADVMPSYRKYDSRHTIVNRIKAGVCEICGEHADYLCMHHVRT
                     LKSLKGRDIFEQKMLKMRRKSLALCPDCFELLHETKESR"
     gene            complement(75128..75628)
                     /locus_tag="NBH55_000084"
     CDS             complement(75128..75628)
                     /locus_tag="NBH55_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HCD1508103.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPS
                     APVRAV"
     gene            complement(75625..76101)
                     /locus_tag="NBH55_000085"
     CDS             complement(75625..76101)
                     /locus_tag="NBH55_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HCD1508104.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(76176..76388)
                     /locus_tag="NBH55_000086"
     CDS             complement(76176..76388)
                     /locus_tag="NBH55_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCD1508105.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(76410..77375)
                     /locus_tag="NBH55_000087"
     CDS             complement(76410..77375)
                     /locus_tag="NBH55_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508106.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            77665..78873
                     /locus_tag="NBH55_000088"
     CDS             77665..78873
                     /locus_tag="NBH55_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEnfa110 family transposase"
                     /protein_id="HCD1508107.1"
                     /translation="MNCVGIDVSKGKSMIAVMRPFGEVVVSPFEVRHTANELSELAGL
                     LKSLDGETRVVMESTGNYHAPVAWLLHDAGLYVSVVNAMLVHDYGNNSLRRAKTDKKD
                     AVKLANYGLDHWLTLPRYIPEEDTRLMLKICYRQYQQYSKVQTMLKNNLISLLDTTFP
                     DANRLFTSPPRADGSEKWVDFVATFWHCECVCGRSEKAFTTQYQKWCRKHGYNFSEDK
                     ALDIYASACRHFGVIPKTDTAKLLVEQAISQLQTTSSALAALKQEMQSLAASLPEYPV
                     VMGMFGVGPTLGPQLLAEIGDVRRFHSKKALVAFAGIDAPPYQSGQIDVRSRSISKRG
                     SASLRRTLFLVMSVILQCAPIDEPVYQFMDKKRSEGKPYRVYMMASANKFLRIYYASV
                     KAYLESLEHD"
     gene            complement(78978..79220)
                     /locus_tag="NBH55_000089"
     CDS             complement(78978..79220)
                     /locus_tag="NBH55_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508108.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKDLLIKVK"
     gene            complement(79468..79770)
                     /locus_tag="NBH55_000090"
                     /pseudo
     CDS             complement(79468..79770)
                     /locus_tag="NBH55_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
BASE COUNT        19701 a        19926 c        16463 g        23734 t
ORIGIN      
        1 acattccatg tattccaatt ccctatccgt tataataaaa gaaacaggca acaggaggaa
       61 aatcatgatc aaatatttta cattagataa tgagaaactg cgtaataccg gaaaggaaac
      121 tgaccaaacg atctggtatt gtgtagaacg tcctaatcaa gaagagattc atcagctgac
      181 acatcaattt gaaattccgt cagattacat cacttccata ttggacgatg cagaaaattc
      241 ccgtacagaa gaattcaatc aatcaaagtt gacaaagcca gcgttattat tgttacaata
      301 tccttttgct aaaacaagtc ctagcggtta ttttcaagtg gatacatacc cgctctctat
      361 tattttgacg cccaaaaaga agcttatcac aattacgaat catgaaccac tgtttttaaa
      421 gaaagtcttt agccagacct ttccaaaaag tgatatttcg cctaatctca atatcttctt
      481 tcaattcatt tggcaggtca cactctctta caacagttat ttagctacgc ttcaatctca
      541 atgcgatact ttggaagcca aacttcaagt atccactgaa aacaaacaac tttatcaaat
      601 catggatatc caaaaaagcc tcgtttattt cagagaagcg acttctgcta atttagatgc
      661 attgaaagaa ctagcgcaaa ataaactttt ccaagaaaat catgctttga aaaatcattt
      721 acgtgatgta ttggtggaaa cagagcaggc aatgactact tctcgcattc aattaaaact
      781 agtagaacaa atgaatcaga ctttttcagc aatcgtatcg aataatctga ataatattat
      841 gaaaatttta acttctttaa cgattatttt aactataccg acaatcgtcg gcagtatcta
      901 tggaatgaac attaagttac ctattgctga aagggatgat gcctttatat ggctagttct
      961 attaacatta tttatttgta tcgtaactac ctattattta aaaaaaggga agtttttata
     1021 aaaaagccct cttgtggaaa tggaaaaaag attgtacact tgaatcaact aattaatgac
     1081 gaggttgaaa acaatgcgtg aaaaaaaaca aacaagtact tttgctaaag taacaaaatt
     1141 tgtcatttgg acaatgctga ttttaacgat tggatctgtt gtattgactg ctgtaatgag
     1201 tgtcatgtaa aaataaaata tagctatcag ctgctgaaaa ggacctgaac cactgcgagg
     1261 tggctcaggt cctttttgcg agtttcaata gttcttttgc atgttcgagt gtgagttttg
     1321 taatctcact tccagccagc atacgtgcaa tttcttcttc tctttctttt gttttcaact
     1381 ctgaaacaga agtttgcgta cgtccatcaa tcactgcttt cacaatataa tattgatagt
     1441 cggcgactgc agcaacttga ggcagatgag tgatgcacaa aacttgagag tatttcgata
     1501 tttgagaaat tttatctgcg atggcctgtg caactcttcc gctcacacct gtatccactt
     1561 catcaaaaat aatacttgtc acaccttgct cagaagaaaa aatagttttc aaagcaagca
     1621 acatccgaga cagttcgcca ccagaagcga ctttcaccaa aggcttcagt ggctctcctg
     1681 gattggtcgt aatgtaaaat tcgatttggt caaatccatc atgattcaat tgtctatttt
     1741 cttcttttaa gaaacgtacc tcaaactcag tgttttccat atacagactt ttcaattcat
     1801 gcagaatgga tttttccaat tcttttgcga tttttttacg cgcttcatga agatctgcgg
     1861 cttgttgctg aagtagaagc tctttttgct ctagttcctc tttcattttg tccaattgac
     1921 cttctgaaaa atcagaagaa gctagctctt cggtgatttc gtcatagtaa gccaatattg
     1981 actcaatgga ttcgccgtat ttacgtttca actgtcggat taattctaaa cgttgcgtaa
     2041 cttcttctaa acgactctca tctaactcca ataaatcaat ctgacgactc atatcgccga
     2101 ttgcatcttg cagcaaatag taagcacttt ggatattttc ataaattgct tcatactcca
     2161 catctagatg ggcgatccct tgcagttctg aaacggccaa gccaacacca tctacactgt
     2221 tttgttcatt attcgtcagt gctccgtaac ctgcagctaa tccatcagca attttttgat
     2281 aattgctaag tttttctctt tcttctttta atttctcttc ttctccgact tgcaaatcag
     2341 cagcagctat ttcttcttgc tggaaatgga gcatatctat tctctgaaca taggattttt
     2401 cattttgctg cacttttcgt accctttttt ccagctcacg ataagagata tattcttgct
     2461 gataactctt tttcttctgt tggaaagcta catctccaaa acgatccaat aaagcaagat
     2521 gtgattcagg ctggagcagt tcctgatgtt cgttttgtcc ttggatatct actaaataac
     2581 tgccgattct tctcagatta gctaatgtga taatatgacc attcactcga caaacatttt
     2641 ttccagttaa ggacatatcc cgtcgaacaa tcagattgtc ctcatctgtt tcgatcccta
     2701 attcaaccat cagttcagag aatccttctt gttttggtag ttcaaacaac ccttcaagca
     2761 cacatttttc tgctccttga cgtatataat cgcttgaacc tcttcctcct gctaacagac
     2821 ctaaagcatc gataataatg gatttacccg caccagtctc tccggtcagc gcggtcattc
     2881 cttcgtgaaa agaaagccgc aattccggaa taatcgcaaa gttagtaata ctgatttctt
     2941 gtagcatcac attcgctcct caacttaaag gtaatgcagg aagtctttct tcaataaaac
     3001 tgcctggtct tcagttcgtg taatcatcaa aacattatcg tcatcattga taatcgaaaa
     3061 taattctttc ttgtaacgtt tatcaatcaa atttgctgct gccgaagcat ttcccggcaa
     3121 tgttttcaaa ataacaaatt tttccatttg gtcaactgcc acgaaagcgt cttttaatag
     3181 tttttccaat ttagtattta catcttcgtt tgtttcagct ggtatgctat aacggtagcc
     3241 gccatctgaa gccggtacct tgatcagttt cagttccttt atatctctgg aaatcgtagc
     3301 ttgagtaaca gatattcctt tgcttttcag gatttcaaca aattcttcct gctttcggat
     3361 atcttgctca ctcaacaatc gagtaatcaa acgatgccga tctttttttc tcattacgtc
     3421 ttcgctcctt agcatattct tcttaatcaa tgatagctta tttccttggt ataataaaga
     3481 agaatccgca tacttctcac ttgattttca cagttacaat ttatttatgg tcaaactgtt
     3541 cgtgtgcatt tgaaaccact tctgccatat taatagctga attgattgtt ccgctttcgg
     3601 gtactttcct caagtgagcc aagaactcga tatttccttc tccccccgtg atcggagaat
     3661 aacttaattc cagcacatca tatccacctt gtgccgcaaa tgacaaaatg tcttctaaaa
     3721 ctttttgatg ggtctgaggg tcacgaacaa ttccgttttt ccctacagct tctctgcctg
     3781 cttcgaattg gggtttgatt aaagcaatca cttctccctc ttcttttaaa atagcatgta
     3841 atggaggcag catcagtttt agtgaaataa aagaaacatc gataacagca acttctggaa
     3901 ttcctaaggt aaagtcttcg ggttttgcat aacgaaaatt cactctttcc attacgacga
     3961 ctctaggatc ttgtcttatt ttccaagcta gctgattgta gccaacgtct aaagcataac
     4021 tcatttttgc gccattttgc aaagctgcat cagtaaatcc gccagtggat gcaccaatat
     4081 ctaacaatgt ttttccttca acagatagat caaatgcttt gagtgctttt tctaatttta
     4141 atccccctcg agaaacataa ggaagttttt tacctttgac tcttaattca ctactcactg
     4201 gaattttttc tcccggttta tcaaaccgct cgtttttttc gttgtaaatc aaaccagcca
     4261 tcactgaacg tttggcttgt tctcttgttt caaataatcc ttgtttgacc gctaatacat
     4321 ctacgcgttc tttttccatt ctttccctca tttctccaca tcaaattgct tgatcatctg
     4381 gcgcatcagc ccgccatcaa aagaaaaaac gtcctcttct aatttgtcta tgatcttgtt
     4441 ggcggataac agctcaatct ccaacgcctc acgtgtcttt tctaggccta ataaacgcgg
     4501 gtaagtattt ttttccatac gttcatcttt accagctgtt ttccctaatg ttttcgttgt
     4561 accaatcaca tcgagtagat catctcttat ctgaaaagct aaacctaaat gttcggctaa
     4621 tcgttgaagt agcaataaga tttcttcagg ttgcttcgct aatatgccac cagccagcaa
     4681 ggcataacga atcaaatgcc ctgtctttct ttcatgaata aatgctaatt cttctaaaga
     4741 aagctttttc gattcgccct cgatatctgc agcttgccca gcaaccatac cttgtgatcc
     4801 cgcacaaaca gctaattgct gaagcagcag aagcttagga gaattaccta agtgtgccat
     4861 actgattagc tggaatgctc ctgtaagcaa accatctccg gccaatatag ctaatgcttc
     4921 gccgaatact ttatgattgg taggttttcc tctacgaaga tcatcattgt ccatcgcagg
     4981 caaatcatcg tggatcaagg aatacgtatg caccatttct aaagcagctg ctacttgata
     5041 gacgggaaca tcaataggct catcaaagga agcaacggcc gttaaaagta ataagggacg
     5101 tattcttttc ccgcctgctt tcactgaata actcatcgca tcatagagtc ctctatccgt
     5161 tgtatgctca tctaaaaaag acaagatttc tttttctaca gcaggtagat gaatagctga
     5221 aaagtcagtt aatctcatct cagctttcct cgtttccgtc aaaatcaact tcttcatttg
     5281 attctgtcat cattttcgtc aaggtctctt ctgctttagc taatgtatct tggcaatgtt
     5341 tgcttaattc cattcctcgt ttaaacgaat ctagagctga ttctaatgga acgtctcctt
     5401 gttctaactg catgacaatt ttttctaatt cttgcagtga ctcttcaaaa gttggatttt
     5461 ccatattttt attctccttt gtctatctct tgtacttgtc ctttaacgat tccatcttga
     5521 taatggatcg tcagcaaatc cccttgatga atctcggtga cactttttac tacttgatca
     5581 tttaacgtcg tatagctgta gcctcttccc atgattttca atggactcaa caaatcaaga
     5641 gattgaacag cttgagcaaa acgctgctgc tgttgtttca tatattgttg gattttctct
     5701 tctaagcgtt tttctagata agcagtttct tggcgagctg ctttaactcg ataaagcgga
     5761 gccacttgtt ccaattggtg gcttaatgcg atcgtttgtt tttctttcgc atgataaatt
     5821 tgctgtgtag cttgatataa tcgctgtttc aactggtcta atttgatggt ctgaccttca
     5881 tacaatcttt caggctgccg aaatacatag gaacgtcgag cacgctcgaa ccgttcttgt
     5941 ttgcgctgga tctggcgtac aaacgcctgc tgcaatctag cttgccgttc ttgaatgcgc
     6001 atcaactctt ctgacaaaac aggaacagct aattctgcag ctgctgttgg agtggcagcc
     6061 cgaacatctg ctactaaatc agcgatcgtg gtatctgttt catgtcctac agatgaaatc
     6121 accggcgtat tagcttgata aatcgcccga gcaacccgct cttcattaaa cggccataaa
     6181 tcctcaatcg aaccaccccc acgaccgatg atcattgtat cgaaattgcc catctcttct
     6241 actctctgga tattcctgac aacattgtcc gctgcttggt ttccttgtac aactgttggg
     6301 aacaaaacca attggacaat tggatatctt cttttcaccg tcgtgatgat atcgcggata
     6361 accgctccgc ttggactggt aaggaccgcg attcgctttg ggaaacgtgg cagcattttt
     6421 ttaggccctt gaaaaaggcc ttccttcatc agcttttctt ttcgttcttc caattcttga
     6481 tataaagcac ctattccatc aggctccatg tgctctacat agatttgata agaacctccc
     6541 gcttcatata atgaaatacg cccaacgaca aggactttca ttccttcttt tggctggaat
     6601 cgtaattttt ggaatgctcc tttgaacata atagcagata tttttgcatg gtcatctttt
     6661 aagctgaaat attgatgggc attggcacgt aaacggaaat tcgatatttc gcctgttaaa
     6721 tatacgcgtt ctaaataagg atcggcatca aattttcttt tcaaatactt cgttaatgta
     6781 gtgacagtta aatattcttg tgccatctat tcacctgctc caaaagcttc ggcagatttt
     6841 acggtttgat acatcagcat cgtgatagtc attggcccta caccctttgg cacaggagtg
     6901 atccagcctg caataggctc cacttcatca aatttcacat caccgatcag cttaccgtta
     6961 gcatcacgat tcatccctac atctatcact acagcacctg gttttacgaa ttcttttgtg
     7021 acaaaatgtc cacgtcctat tgctacgact aaaatatctg cttcttgtgc cagttcagcc
     7081 agattttcgg ttttagaatg agcaacagta acagttgcat ctgccatcaa gagtagctga
     7141 gccatcggtt taccgacgat attgcttcgt ccgataacta ctgctcgttt acccgccgga
     7201 tcgatagaat aagcttcaaa cattttcatg atgccgtatg gagtacaagg aatcttgtcc
     7261 ggtttacccg caaggagtct gcctaaattc atcggatgaa aaccgtctac gtctttttca
     7321 gggcggatag ctaacaggat cttttcttca tctatatgct ttggcaatgg taattggacc
     7381 aatataccat gaaaactagg atcttgattg tatttttcaa tttctgcaag tagttcgtct
     7441 tcagaaatcg tctcaggata acgctctacc aatgaatgga ttccgatttc ttttgctaca
     7501 cgttctttat ttcttacata gatttggctg gctggatttt cccctaccaa taaaacgact
     7561 aatccgggat ggataccatt atctttcaat ttttggattt tttcagcaat ctctgcctgc
     7621 attttttcag ctaattcttt tccgttaatc aattctgcca tctaaccact cccagatatt
     7681 ttttgtgttt tcattctacc atagaatttt cccgatttat aacaggttta tgatgattca
     7741 tagaaaaaag ctaggactta cgccccagct tctaattctt tcaatacatt ggataaaaca
     7801 ccgttgacga atttacgtga acggtcatca ctaaatgttt ttgccaactc gattgcttca
     7861 tttaaagcaa cagccgctgg tacatctgtc acataagtca tttcaaaaat cgcgatacgc
     7921 aagatcacca aatccatttt tgagatccga ttgatgtgcc aattgttttt taaatgtttt
     7981 tcaatcactt tatccaattc atcttttttt gcacagacac cgccaactag cgtatcaaga
     8041 taaacaggaa caaactctga ttcatcttcg ttgatcatgt cgcggtggtc taattcgatt
     8101 gcgttgaaaa tcgcatcttc ttttgttagg tctgcattga agtctaatgg aaataaagcc
     8161 tgcaatgcca tttcccgaat ctcgtgtctt gataattctt tattcattct cttcctcttc
     8221 gtcttcaaaa agttcttcga ttgttggttg ttctaatttt tcaggaacaa cagctactac
     8281 atggatattg acttcagata aatcgatatc cgtcatgaaa aggacttgct ggctgacccg
     8341 ttcctgcatt tccattgcta ctttaggaac tgaaacacca tattctagat aacagtaaag
     8401 atctactttc aagccatctt cctcgtttgc taagtaaaca ccttttccgt gagctgcacg
     8461 tcctaataat tcagtgacat tgctggcaaa agttccgcgc attccgtata cgccttctac
     8521 tttagaagct gcaatcccga taattacttc gatgacttca ggagcgatca cgatctctcc
     8581 taaatcttgg tttgcgttta aaatcaaatt tttttcatct gccatttatt tattcctccc
     8641 tttgcctaag cacgagatac atccgaattc tctatgatgc ggcatagtga tcacgtggta
     8701 agaaaactgc cgccgatcag ctgcaaactg ctatgaattg gagaaaacac ccaactttat
     8761 tatctcttac agcttaaaag ttcttctata gtgtatcatt aaatcgagtg attttctata
     8821 acaaagtctg tgaacactga tttttacgct tacttcttct attgatgaaa ctataagatg
     8881 atcaattctt ttggtgaacg agtcaacaca cgatttccat cttctgtgat caacaagtca
     8941 tcctcaatac gtactccacc gattcctggt aagtagatcc ctggttcatc agtgatcaca
     9001 tttcctggta caaattgctt ttctgcacga gaagaaacgt ttggtccttc gtggatctct
     9061 aaaccgatcc catgtcctgt agaatgtcca aatgcttccc cgtaaccatg ttttgaaata
     9121 taatctctag caactgcatc taattggaca ccagtcattc ctggttttgc agcatcaata
     9181 actgctaatt gagcatctaa gactatttga taaatttctt ttaatttttc cccaggatca
     9241 ccaatagcga atgtacgtgt catatcggaa acgtaccctt cgtaatagca accaaagtcc
     9301 aatgtgatca agtcaccttg ttcgatgatt tttttgcttg ctacaccatg cggcatagcc
     9361 gaacgcaatc cgctagcaac gatcgtatca aaagaaacac ctgaagcgcc aagagaacgc
     9421 atataaaagt ctagttgatt tgcgacttca atctcagtca tgcccggttg gatcattttc
     9481 aggatgtgat catatgccat atccgcaata tgacatgctt tttcgataat agcgatttct
     9541 tcttcatctt tcacttctct gagttcttcg atcatctgtg aaacaggtac taattccgca
     9601 tcgatgattt cttctaaaac cgaatattct aaaaatgaga ctgttgtttc ctcaaaagct
     9661 aatgagtgca gttcttcttt gcgtactaag tttgctacct catcaaaaat aggtccgaca
     9721 tttttaataa tttcaaaccc ttgagcctgc gctgccgcct gctcggtata acgaaaatca
     9781 gtaataaaaa atgctttttc caaagtaata acagctaatc ctgtcgtacc tgtaaagttc
     9841 gttaagtaac gaagattgta cggactggta accaaaaatg aatccaaatt ttcttcttgc
     9901 atttttttgc gcaatttttc tacacgtagc atcataatga aactccctct ttctttgcac
     9961 gcttttttcc attatagcaa acttttcttc gtacacgaca gttaaataaa aatttccgga
    10021 aaacttcttt ttattttaaa taaataaagc caaaaaggag tcacagtctc gctgcaactc
    10081 cttttagttt actattttgt aaaaacttat gatcgttttc caaagaaaaa tgagacaact
    10141 gctaccaaaa tcactgcgcc taaaatagat ggaataagtg ccatacctgc agcttttggt
    10201 ccccattcgc cgaaaatcgc ttgtccaata cttgaaccga ctagtccagc aatgatattc
    10261 gttacacagc ccatcgaatt tcctttactg gtgatcgcac ctgcaattgc tccgatcacg
    10321 gctccaacaa tcaatgtcca taaccaactc ataatataac ctccttcttt gctaatcaat
    10381 tttagcatat gggatttaaa ggggacaaat aattggtttt gctatttcat attttcttct
    10441 gcaaagtaaa aaaataaaga aaacctcctc ttagaagttt tctttatttt ttacttgcgt
    10501 ttaaacatac tacgaatacc acgtttcact acatcaaaga aactcaatga ttgtaccatt
    10561 ctatcttcat ctacatattc tctgatcgca cgaagcactt ttttaggttc tttggaagaa
    10621 aatgtgtatg taccattttt tttcgtttga atggcatatc gaggaatcca ttttcccttt
    10681 agcatgactg atgctagtac ataatccact tcttcccatg gaatttggat atatttgcgt
    10741 ctatctcgcg catggtaaaa ctcaaaacca ttatcaccta tcatgatttg cccgtaatct
    10801 gtcagacccg taaacgaagt agcatcaatt accaagtcta cttttgtatt gattgattga
    10861 accattttct ccctccagat atttgtttat gaacagtata ctatataaaa aaagctcggg
    10921 acaacgcccg agctatattt ttatggatta taaaagtccg atgacgtgtc caacaacacc
    10981 gactacgaat aaaccaagga tgataacgat tggagaaacc tttttcttaa gcaaccacat
    11041 acataacaat gttaatccta atgcagctaa accaggaatc aattggtcta agttgtcttg
    11101 taatgttgtt accttttcag gagaaagggc taagccatta ttaacttgtt caaatgcact
    11161 cttgattcct tcaccacctg aaggcaattt atcccattcg atgtaagcac ctttatctaa
    11221 ttttacagtt gatacaactg gtgagaattt gattgatacc catctttgta caagggcagc
    11281 taatacgaac atccctaaaa ttgaagcacc ttttgttacg tcttgtaaca atccgccaga
    11341 aaggtcatca gtaattttag aaccagcttt gtagccaaat tcttgtgtat accacatgaa
    11401 tgcccaacga ataatgttcc aacctaagaa gaagatgatt ggtccaagga tatttcctcc
    11461 catagctaat gaagcaccta gtgcacctag cattggacgt actgtaaacc agaacactgg
    11521 gtcaccaaca ccagctaaag gacccatcat acctacttta accccttgaa tcgctacatc
    11581 atcaacaggc gcgccattcg cacgttcttc ttccagagcc aatgtaaccc ctaaaattgg
    11641 agaagcaatg tatgggtgtg tattaaagaa ttccaagtga cgttttaatg ctgcagaacg
    11701 ttcttctttt gttttgtata attttttgat tgctggaatc atagaaaatg cccagccacc
    11761 gttttgcata cgttcgtagt tccatgaacc ttgaatgaag gtagagcgcc acgcaacagc
    11821 taaacgatct ttttttgata atttgatttt ttcttctgcc attttatttt cctccttcaa
    11881 gattaatagt cattcaaaat gtcgcctaaa gggtcaccag agttgcttcc gcctccatta
    11941 tttgatgaac cacccatttt agaaaggttc aagtaaataa gagctaatgc aacacctaaa
    12001 gcacctagag caatcaatgt taattgagaa attgcagcta caacgaaacc aataacgaag
    12061 aatggccata cttcttttgt agccatcatg ttgattacca aagcgtaacc tacagctacg
    12121 accattcccc caccgatagc cataccatct gttaaccatg ctggcattga ttcaaggaat
    12181 gattgcacag tttgtgctgg gataaatagc aacgctgctg ctgggaccgc gatacgaacc
    12241 ccttgtaaac atacagcaat gatgtgccac atttcaacct tacggatgtt tccttcttca
    12301 gcagccgcat ccatcaaatg aacgattgga acagcgattg tacgtacgat cattgttaag
    12361 aacaaccctg caacagccaa aggaacggca atcgcgatcg cagaaggaac acctttaaca
    12421 ccttgtccac ctaaaactaa aataattgct gatgctactg atgctaatgc agcatctggt
    12481 gctacagcgg caccgatatt tgcccaacct aaagcgatca tctgaagcgt accgccaagt
    12541 acgataccag cttcgaggtt accggtaact aaaccgatca atgtacatgc cactagtggt
    12601 tgatgaaatt gaaattcatc aagaatacct tccataccag ctagaaaggc aactaatacg
    12661 actaaaatta ttgaaataat agacatgata agcctcctat aaattttaat attatttatt
    12721 acgcttttgc taattcagct tttgcttttt tcaaaatatc atccatgtta tcgcgtgaat
    12781 cattaggtac tttacgtaca tcgaatttca cacctttttg ttcaagcttc tcaaacgctt
    12841 caacgtcttc ttgtcccata gacaatactt tacttacaac gactttaccg accgaatgcg
    12901 ccattgaccc aacatttaat tctttgatgt ctacgccacc ttcaacagct gtcaatacgt
    12961 cttcaggatt ttcaaataaa agcaatgctt tggtatttcc aaaacgaggg tcttttgcta
    13021 cttcgatcat tttgctaata ggaataacat ttgcttttac acctggagga gcagcttgtt
    13081 cgattagtct cttacgcaga tcatcctttg atactgcatc tgaaacaacg ataatacggt
    13141 ttggttgcac tgcttttgtc caagctgtcg caacttgtcc atgtaacaga cgtgagtcaa
    13201 cacgtgctaa tacatatttg atttttccgt ctccgaccac agttccttct ggaagtgctc
    13261 ctttaggttg tgcgtcttca actgctgctt tcggtgcttc tgccggttct aattcttccg
    13321 gttttacttt tacaccgtct tttgctgttt caatgatgtg tgctgcaatt tcatgagcag
    13381 attccattga aaagcgtgat gcgtaggctt caatcaacat tggtaaattc aaaccgctga
    13441 cgattgccca tttatctttg tgctcttcga ataatgtgtt tgattgattg aacggtgttc
    13501 cgccccataa atcaactaag aataacactt cgtcttgatt atcaaaagaa gcaaccgctt
    13561 cttccaattt ggctctcaag tcatctggac cttcacttgg tttcaaaatc acggctttga
    13621 cgttttcttg ttcgccaaag atcattgaac cggattgtaa gattccttca gcaaattgtc
    13681 catgacttgc taggataatc cctaccatat ttatacctcc tatttacttt tgttgtaaca
    13741 aattcaaaaa tattaataat tgctaaccat tacaacagaa ttatttgacc ttttccaaca
    13801 accattcttg ctacaccttt ttgattttcg ctagtagttc catcaaaagg attttcctgt
    13861 cagcaggtac ttttcgaatc tctaactcca cttcttgact gttcaagtaa cgaaaggaat
    13921 caatatcttg ttgatttact gaaacaaaat tggtaatcat tgatttgcct tcagtaaaac
    13981 tcataccgcc gatattcaac gacttcaact gtacaccgca gcggacaacc gcttcaacat
    14041 cttggggatt ggtgaataaa agcatcactt ttgctcgtga taataatcca ctatgataga
    14101 tttgaatcat tttctgcacg gttattacat ttgctgtgat ccccggcggt gcagcttctt
    14161 ttagtagaaa ctttctcaat ttatcttgag cgactacatc gctgatcaca atgatacgtt
    14221 caacacctgt ttgcttcgac caaactgttg cgacttgccc gtggattaaa cgatcatcta
    14281 tacgtgccaa tcgaatatcc atctgcaaaa ctccttcttc ctcactactt cacagaaacc
    14341 cctatcattt ctgtgtgaac aaccacctcc tagcacacac tactataagc aaaaagcgtg
    14401 ccaacttttt tacgatacac taattgaatt taaaggattc ttaaccaata cacaaaaacg
    14461 atacactata aaagtgtatc gtttttgtgt attcatggca cagtgtattc ttttttatct
    14521 aactgattat ggactagacg tagcagataa tacatttctt ctatcggcac ttttactttt
    14581 atctgatctt ccaacagaac aactgaggct gccaattggc tataaagagg atcattcgtt
    14641 gtatttgtca attcgttttc ttccacagtc aatggttcat tccgaataat acgttcgatc
    14701 attcctgctg tgtgcatcac taggttgatt ttgaatccat attcttcatc gccgattccc
    14761 gtcgtctgag agatttgtgt agaaaattgc cacattggat cgattaactt actaccatta
    14821 ataaagatga agttatcaga gataaactca acacacgtat ttttagctga ctcttcatct
    14881 agggagatga attcagtttc ctccaattct gattccatca gcaactgatc caaaatgact
    14941 tcgatatttt gatcaataaa acgttctaaa ggaataaatg gggcttggat attcaaatca
    15001 gtaatcccag tagtagctat gatctgatat tctttttgga taactggcag ttcttctttc
    15061 acttccacaa tcgacaaagc tagaacttct aattctgttt cttgacgctt gtttaccgct
    15121 cgttcgatca attctttgat tctttgtgct gtaccttcgc cagatgcgca gatagccaaa
    15181 acagcttttt tcttatgaat aggttttgct tcctgaacag attctacaga agatatttct
    15241 gcgtaacctc gaaaattttt caatgattca tgcaacatat ctagatctgt ccctaatact
    15301 gagacctttc gtacagtttc caaaactaga gaagttgtta ccatatcaac agtacgtact
    15361 ggaattccag tgtctctttg aatttgcgaa ttaaacgacg caagggaccc catatctact
    15421 aagagtatga ccccgcttcc ttcatctact tcttgtacat tgcgttcaat tcggtccaat
    15481 gctgttttcg gatccatatc caaaggcatg tctactgctc gtacgttctc tgcatctaac
    15541 aactgttgga caacttggac catactgctt gctgtgctgt ttccgtgagc tgcaacgact
    15601 acaccgattc tgccgcttgc ttgatttgcc cgtaacgaga cgagtagaac ggtcaagtaa
    15661 tagtcttcac tttctggaat ctccacttga aaatacgttt cgatataact acgcacttct
    15721 ttcgcaacag catattcatc tggaaaatca aaagccatct cacggatgtt atcattcgta
    15781 tgccgttctt ctcctaagtt gatttttttc aaaaaagaac tgatatgcaa gctcatagca
    15841 taaataaagt tttgctgaaa ctcatatggt aaagatcttt tgaccattac ataaatctga
    15901 ttggtcactt ctatgatttt cggatctaca aattctgcta atttgctgtc tgcattaaaa
    15961 gaaaaaccat ggtctttata gaaagacttc agatggatgt tgatatctgt agaaatgaaa
    16021 tgattgattg cttcttgatc caaaccatcc gacttcagta aagctgcttt atctccgatg
    16081 atatcatata gattataagg taactcatag gagtccgatt ggattttcat gatcgtatca
    16141 tttggtgcta cagttatctt tggctccaat attttagaaa gctctgccat tgcactgcgg
    16201 ttgcttgcta attgaatcaa tccgctacga atcccttctg tcaaatcatc gattgtgatc
    16261 gagatttcgg gtgattgcat atgatttaga aatcctcgtg cacatactaa ctggatattt
    16321 gacttcaatt gtcctatatt tccataggtc acactgccga tcaatgcttt tgctacatct
    16381 tctgtcaaag aaatctttcg ctgcgtgcgg tttgcttcgt gggcaatcat gatttttact
    16441 aaatcgactt tctcatttgc aggacgtttt tcaaaagccg gaagtttgat attgattggg
    16501 atacgccgaa caaaagtttc taatagagaa gaacctgggt cttctgtggt cgcacctacg
    16561 atccgtacat ttgcttcatg ggatttcgtt gtttccccaa gacgactgta agttccatga
    16621 tccataaagt aaaagatcat ttcttgacct tcaggcggca accggtgaat ctcatccaaa
    16681 aacagcatac cgccatccgc ttgatcgata atccccattt tttcttcatc tgcgcctgta
    16741 aaagcacctt ttgcgtaccc aaataaatga ctcatcaaaa gctcagggtt attggcataa
    16801 tcagcacaat tgaaaacaat cagttccgtt tcctcatcta ttacatgatg cgcttttgca
    16861 aaatgataca tcgcgtgtgc aaaataagtc tttcctgatc cggtaggtcc tgtaatcaaa
    16921 caatttaatc ctcttggcgg atacaaaatc gcagcttttg cctgttccac tgaatttttc
    16981 atgctgccgt tagctccaat gatttttgca aagatatcct tagtatctat ttggagtgta
    17041 tttttttctt cagagaccgg cgtttctttg taactgacta catgttttgt aactggcttt
    17101 acgtgcgtaa cgagtgtttg atatatgtaa cgtactggtc gtccgtctgt ctttatcagt
    17161 tttccttcgc gcactagctg attcagatct ttactgctat ttgttcgctg aatccctaac
    17221 acttcagcaa tttcctgtgt tgttactcct tgatcatatt ctgcctttgt taaatgagcg
    17281 gttttttctt tgacatacat ataaattcgg tcaatgcgtt ttgccaatgg tatcgctcct
    17341 tttctataga ctattgtact taggcttcaa aacataatac tatgtattta tttctgtttc
    17401 gtcaactatt tacctgattt tgttttgttg ataacacgcg gatagataga aatgttcaat
    17461 ttaaaagaac gaaaaatccc gatacgattt ttataatgga aacaagaaaa agaacactgt
    17521 ttaccatata attatttcac tgatagcaaa caatatagct tccatcaatt gaaaacgata
    17581 gttatcaaaa catcctgatt ccaaattggc aaaaaaatat taagtgtaaa aagacaaagt
    17641 aaacttcgta gcctccacta caacttttat ttttataaac aaaaaaggct gtgacaaaag
    17701 tcttgtcaca acctctttaa tcataagatt caattaagct tcttgagcta ctggataaac
    17761 agacacttgt tttttgttac ggcctttacg ttcgaaacgt actacgccat caacttttgc
    17821 aaataaagtg tcgtctccac cgatacccac gtttacacct ggatagattt ttgtaccgcg
    17881 ttgacggtaa agaattgatc cacctgtaac tgtttgtcca tcagcacgtt tagcacctag
    17941 acgttttgat tctgagtcac ggccgttaga tgtagaaccg ccaccttttt tgtgggcaaa
    18001 taattgtaga ttcatagata acaacatggt ctgcacctcc tatttatcat taattgtttt
    18061 aatttggata tattctgagt tttcagcttc tatcgattgt aatcctaata aaagattttc
    18121 taatagaatc tcagcaatat tgttttgttc ttgtgtcatt cctgaagtga cttcggtata
    18181 aagataaccg ccttcttctt cattcatttc aacaatcggc tcaaaaccgg ctaatgaggc
    18241 aatcccgttg acagtgctga tcgctaacgc agaaacaccc gcacaaacga tatcacttcc
    18301 ataaggacca gcatcagcat gcccggtcaa agtgaatgaa acgatctgac ctgcgtcatt
    18361 tcgcttaaat gttcctttga tcatatatag gccttctttc tcaaacgcta ttaagcgttg
    18421 atcgcgtcga taaccacttt tgtatatggt tgacgatgac cttgtttacg gtgtgaatgt
    18481 tttttaggtt tgtatttgaa agtaacgact ttcttttgtt tgccgtgttt ttcaacagta
    18541 ccttcaacag ttgcacctgc aacagttgga gcgcctactt ttgtagattc tccgcctact
    18601 aagataactt cgtcaaaaac aactttttct cctgcttcaa cgtcaagttt ttcaacatag
    18661 attgcttgtc ccacttcaac ttttacttgt ttaccaccag ttttaataat tgcgtacatg
    18721 ctataaagca cctccttctg atatacttag actcgccatc cgaagtgaca gcatatgctg
    18781 tgcttattac ttgttctgag cggttgtagc tgtggagtcc acaattacaa cagtactagc
    18841 ttaccagaac agcaaccgtt agtcaactaa atttaaaatc aaaaagtaaa acaaaaattc
    18901 aagcaatact tctgcttttc tatcctcaca gttctaccaa ataaaatagt cgcattgtgt
    18961 ttcttttatt cttataataa aagaaaaagg aggagcataa atggaatacc caaaagaact
    19021 gatacaagaa gcttctttaa gaatgaacgg ccgtcctgta gaaggattta tcgctgggca
    19081 aggacctctt catcctaaac tgatgctcgt gggagaagcc ccagggaaaa cagagattga
    19141 tacgcatgtt ccttttagcg gtcaagctgg aaaagaattg atgcaggcac tttcttcaac
    19201 tggcttaaca cgagaagagg tgtatatcac cagtgctgta aggagccgcc cgtatcgcgt
    19261 gactcatcgg atcaataaac ggacacaaca gtcagaaact gtttatccaa atcggacacc
    19321 tactcgttcc gaagtatttg cgcatgcacc aatattggat tacgaactga tgaaggtcca
    19381 gccaaaatta attgcgacac ttggaaacat tggtcttcaa cgtttacttg gcaaagaata
    19441 tacggttacc aaaaatcatg gtcaactcta tacagggcct gtcttacaat taacagaaca
    19501 aaaggatgcc tacgaatggt cagaaaaaaa ctatcgaatt cttccacttt ttcatccagc
    19561 agctattttc tacaatcgct cccttgtacc tgttatccat caagactggc aagtactagc
    19621 ttcactttta cagcaatatt aaaaagaaca gacaagacca taaaatcttc agtttacagt
    19681 cttgtctgtt ctttttcatt gttttaatgg ctaattgcag tatatttcgc atgtaaagct
    19741 gtcaacgcat gagcaactgt ttgatgttga tgcgtgaatg tatgttccca caagtgagaa
    19801 agttgttttt ctacttctgg ctgcaaggaa gtatagataa tcgtttgtcc tctttgctct
    19861 gctttttctt gaaattcatc aaaaactgca cgtgccgtca aatcaatact tggtacgcct
    19921 cgcatggaga aaatgacacc atcgtgatga tttagttctt ccaatttatt tttgagtttt
    19981 tctgaagaca taaaaaacag tggaccacta atatacacta ctgtccagtt attcagcttt
    20041 tccgtttctg gcagccccat tctttgccaa tcgatcgttt ctaatgaaat cgtgatagca
    20101 gcgcttttga caacaaaaaa tacacagccg ccgatcaccc cgatcacgat agcaatactt
    20161 aaatcaaaga taactgtaca aatcatcgta aggataaaca atagaactgc tgaccaatat
    20221 ttattagtaa atagctcctt gatcgtttcc cattcattca tacgccaagc cgtcacaatc
    20281 aaaacacctg caagtgctgg cattggaatc tgtgacatca ctggcgcaaa aacaagcatg
    20341 gagagcagta agacaactgc atgtatcata ccagctaatc tagtttgtgc accagatttg
    20401 atggccacgc ttgtacgggc gatggccgca gtagcaggaa tcccgccaaa gaaaggaagc
    20461 aacagatttc caattccttg cgccattaat tcttgattgc tatctaaggg cttatttgcc
    20521 attcggccag ccgaagcacc acatagcaag ctttcaatca ttcctaataa agcaatgcta
    20581 atagctggaa ctgccacttg ttgcaaggca gaaaagctaa aatctcccat attcaaacga
    20641 ttactactga tcaatgtttg tggaatctta cccacagttg ctactggtaa atggaacaat
    20701 agcataactg ctgttgccaa aatgatagcc agcagcgaac taggcattcg tttcgcccat
    20761 ttttttggat agataaacat ccctaaaatg actagccctc ccatgatcaa agaggctgtg
    20821 tttatcggaa atcctatcgt ttgataacta cttagttttt caataacgtt tgtaccttcc
    20881 gagtgaacac caaataaatt atcgatctga cctaatgcaa taatgatagc aatacctgaa
    20941 gtaaaccccg taattacggg tgctgggata aaagaagtca gtgttcctaa tcggaaaagt
    21001 cctgctgcaa gtaaaaatag acccgctaaa aaagtagcaa gcaatacacc ttgcattccc
    21061 tttgttgccg caatagacat caagatagct gccatcgcac ctgtcggacc tgatatttga
    21121 taaaaaccgc ctgataaacc gccaataacg ataccggcaa tgatagccgt aatcaaacct
    21181 gcggccgcat cagctccgct tgatacccca aaagccaagg ctaaaggcaa tgctacagca
    21241 gctactgtga tcccagccaa aagatctttc tgaaatcttg aaagatcata acctgaaaat
    21301 tcttttttta gtgaggaaac ataatttttg aacacctatc taactccttt ttcatgaaat
    21361 tttctaaaat accattcatt agaatatcat atttcatgag ataaagtaat atttttcaca
    21421 aagtttcctg ttagataaat tcttattttt tctatattta taagaaaata gaaagcaatg
    21481 ccaacagagc tagcccacct tgtttgagta aaatccaacg atcgctagtc aaactgccat
    21541 ataatgcaac aaaaaaaata gatattaata gcatacttgt gatttcttta gatgcggaag
    21601 aaagataagc tccataaagt agtcccactc ctaatagtcc attgtacact ccttggttct
    21661 taaacaatgt ttgaacagat tttctgctta actcttcttt actcatttta aaaaccctac
    21721 ttgtcgtgtc agaatcagtt ctcaccgttt cgatatacat gatataaaaa aattcaattg
    21781 caactagagt cactaaaatt ctagatagta tcgtcatatc ttcttcctct tttctgatga
    21841 gttttatttt tttgctcctt ataattgtac tcgataagga tgttttttta ttttatttgc
    21901 ctttgaaaac aaacaatatt ggcacaaaaa acaaagaaaa accgttagaa acctcagttt
    21961 ctaacggttt tttagcgtca caggagggat tcgaaccccc gaccgtccgc ttagaaggcg
    22021 gatgctctat ccagctgagc tactatgaca agaatcagcg gaacatcaat aattatagat
    22081 aattaaattg cttctgtcaa tacgaaatag ttatttaatt agcaaataat gaattatttt
    22141 ggcccaacta ctaaatcaat cttttaaaac gagacattct ccttcagctg ttctaaggtt
    22201 atcaattgaa aacaggagct acattgactt catgctactc ctgtttattt agatttcctg
    22261 tatcctttaa ttgacttaca aatatagaaa cttttacatc aagttcgaat tgagccctcg
    22321 attcaatcga ttatctacag gctgccataa tcatgtatca caactcgatt gtttctgatt
    22381 cctttgtcat taagatcaca ccatcaccta atggcaagat tgtcgatgtc aaatcgggat
    22441 gatccatcac aacacgaaga aattcattta atttacgatg gatagctcgt ttgccacggg
    22501 gaatctcttc atctgggagc aagatcgttc ctccttggaa gatgtcatct accatcaaaa
    22561 cgccaccttt tcttaacaaa cgtaagcatt caggtaaaaa ttcaatatat tttgattttg
    22621 cactatccat aaaaataaag tcatacggac cagacaactc cggcaagata tctgctgctt
    22681 gaccttcaag caaagtcact ttatctgtca gtcctagacg ttcatagttt tttttagctt
    22741 tctcgatcat gacatcgaaa cgatcaattg tcgtaacatg agcgttttct ccgattgtcg
    22801 tggccatgac acttgatgaa aaaccgatag ctgctccgat ctccaagatt cgttcaggct
    22861 gaagctgtcc aagtaaaaat ttcaaaaata caactgtttc atggggaata actggtactt
    22921 ctgctacatg agcttcttct tccagtttgc ctagctcacc cggtaatttt ttttgctggg
    22981 tacgcataaa atccaatagt tcttctttga ctacaggacg atgcatcatt tcattgcgca
    23041 tactactcct cctcttcaca cattatacga acaactgact aaatctacaa gctgcatgag
    23101 tgttttctcg tgtttcatgt tcagaagagt cgttggatct ctggaataca tttatgtttt
    23161 taacattctt acaaaaattt ctgtgttcgt ctccgcttct ttttcaactc tacaaaggta
    23221 ttccatgctg tgattcctgt taggcaaaaa gcaatcgtaa agaataaccc ctgaatccat
    23281 gcaaatgccg tttttgaaag gactagtcca atatatggaa tctgaaagat gggactaccg
    23341 ataatatttc tcttttttac tactccaggc tcaatataag tctttgtatc agcttttgag
    23401 gaaaaattat atgggatttt agattatctt tttagaagtt cttgaatgtc ggtatagccc
    23461 gtgtttccgg gcttttccgg catttttgat attgttagat acttacaaat gttcttatta
    23521 cctctcacca tttccccctc aaaagtagta aatggagtag taaaaccggc cttgtgtcag
    23581 gctgccagcc gttccatttc tgcccttgcg gaatcgaatg tggcgtgtgc ataatagttc
    23641 agcgtcatgg tgatattgga atgtcccata atgtactgca aggctttggg gttcattccc
    23701 gcattggcta accgggtgca gaatgtatga cgcatagcat ggggcgtcgt cgttttcggc
    23761 aacgcttctt catggctctt gttgtacttc ttcacaagcc tgccaaacat cccgccataa
    23821 tctaccgccg tcatgggata gccgttctgt ttgagaaaca ggaaattggc atacccgtca
    23881 ataatgaacg gctttgcacc tttgcggttt tttatcactc gctggaacgc ctgatacact
    23941 tcctcattca tggggatttt tctgacgccg ctttgggtct tgggttcgtc aatatagtaa
    24001 ccgacgccgg tatttctcaa aagctggtgg ctcacgatga ttacccggtt ttcaaaatcc
    24061 aaatctttgt ccgtcagtcc gcacagttcc gaaatgcgaa gccctgtccc cagcagaata
    24121 acgattgcgt cataatgctt ttggtaaacc ttatcacctt gaataaagga cagaaaactt
    24181 tcttcctgtg ctggtgtaag aggcaccttt ggttccgtat cgtcgtccag aacgtcacta
    24241 agctggaagt caaacgggtt ttttcggatg caatcgtcct gtatggcggt gtaaaaagcc
    24301 gctttcaggg aacgcttgtc gttattgatg gtcttataag aaaggccctt ttctttcatc
    24361 cgaagcgccc attcctttgc gtcggacagc ttcacactgt caatcgggca gctccccagc
    24421 cgatcttgtt ccagaatacg catcagccgt tcccggccca gtttggtgtt gtgcttcaca
    24481 ttgccccggt gcctgatctg cttggcgtag agctggcaga cggtcatttt cttgccgatg
    24541 gtgtctatcc cgtcgtcaag gtctttctgg atttctttct ccttttcccg gagtgaaata
    24601 tcctcccgtt tacctgccgg ggtcttatcc gtaggcacta acttccatga gtaaacaaac
    24661 tgcggttttc caaaggtatc aacatatttg taagcatatc gtccgtcttt tctctggctc
    24721 tctccagacc gcaaaacgcg atttttgctg tctcgtcttt tttctgacat ctcgtcatgc
    24781 tcctttccat gacggaaaga gccgcatata tgcttataaa ttattataac atatctgcgg
    24841 ccctttttca ctattagatt gtgtccaaag tgtcaattat tttctcaaac tgcttgcgtt
    24901 tgatctggat gcggttgcca ttcataatga cccagccaga ggccggattt tcctcggcca
    24961 gcttacgcaa tttgttttct ccgatacgga aatatttaga cgcttcctca atcgtcagcg
    25021 tgtatttttc ccaaataggc acatcatggt tcataatgca gccccctttc acaaattcaa
    25081 aggtttgtgt gatttagggg catagacgga cggctgctgg cacagctcca cgggactttc
    25141 acccccgcgt ggttctcacg cggccctacc cattgcctgc gacgctttta acgctcggac
    25201 tgtggctata agggagtatc atagtcctgc ctgtacgtcg tcgcccgcaa gccgccacgc
    25261 tcacattgcg gcgtggtgtt cctcgctccg cttttcccgt agggaaaagt atcatggcgc
    25321 accagccgtc cggctcggca cagacagaat gtgtccgtat gccctatgat gcgccgccgt
    25381 tatcttgcgg gcatatttat caaggtgcga tcaggccacc gggaaaacac cgaaacgggg
    25441 aaagggtaag ccgtttcatg ctgccggggc cggttcgtca gttcgtgacg atagcgtgga
    25501 tgagcttgat ttccagacgg cgcttcatgg cctcgtccag acacacatgc ggatagccgt
    25561 tttcgtcata gagcgtccgg gtacacagct tgttcatgta accgctgtaa tactgcaaaa
    25621 cgcgctctac ggctccggca tccccagcgc gggcggcctc gatcaccgaa aggggcaaaa
    25681 gctcaatgcc cttgtagttc gggttcttca tccgcgtcta acctcccttc ggcatcaacg
    25741 cctttagctg tttccgcagc tccgataagg tatttgtcct gtgccgctga acggcactcc
    25801 gggacattcc cacaaggccg ccgatctcac catctgccat atccagaaca cagtgcagga
    25861 ttaaaatact ctgctccatg ctgggcagcg cggcaaaggc ttcggcaaca agctcattgt
    25921 tgatatgtag gtcatagcca tgcgacgaaa acgtaaagct gtcgctgggg tagtgatcta
    25981 ctgtacagag cttgtccaaa tctgcctgtg acaaagagct tagtgacact tcacggtcac
    26041 ggcggcgctt catttcagcc aagtagcttt tggcctcgtt acgcagtacc gtcttgcaaa
    26101 aagcatcgaa catgcaccgc gcttcatagt cgcgggggat agcatccatc ttctcacccc
    26161 ctttctgtgg gggacttagg tggttctctc cctttccgct aacaagactt tgacaaggcc
    26221 gccagctacc cgctaaatac ctcctttttg aaaatttctc aaaaaatttt tttggggaca
    26281 gccgggccgc aaaagacgac aaaaatcgcc cggcaggtcg caaacctacc gggcgagaaa
    26341 atgctttgct atgctgctga tttacatggt atagtacata ctcataaccg gaatagccct
    26401 gtggaggggc aaggcttttt tgagtggtgg aagtggatta gagcatggca atagcaaaac
    26461 agcatagcgg catcctccta tgggccgcca caccttttgc tgccagaaaa gaacggcggc
    26521 cttgatttct caaaaccgcc gccagcaaaa tacaaaatgg gcgtgggaaa ggtctgccca
    26581 acagcggttt ttttcttttc tgccgctggg cagattgaat actataattt gcaatgtatt
    26641 acaaaagatg ctttaaccac tttccagtaa aagattgtcc atgaccagca tagattttat
    26701 caaatccaaa atctgataca aaaccaatac tttcattatg aaacggcaaa attaaaatag
    26761 gtatcggggt atggctcatc aatcaactta tagtgccacc attcaaagcg ataagactga
    26821 aatccgctgc tttccatgat tttacgcaag catcttcgat tttgtgcctc tgcatcccct
    26881 atcccttttg ctgtatgatg cgaccgttcg tccataaaat cgaagttact tcccattgaa
    26941 acaagttccc ctgtatccag gtggtagagc gtaagatcaa ttgcgcttcc acggctatgg
    27001 ctggattgtg aggccacata accctttgta atcaactcgg ctcgctctat attggggtaa
    27061 aatttttctt ttgtgagatt gtcctccggt tgcgctgccc aacgcaggaa gcagtccacc
    27121 gcagttcttg gccgatatcc atcccataaa agcaagccat agccaagcgc agccgcatgg
    27181 atttgtgcct cgcgcagagc aaacgccacg gcctttgtgc cgatgatgcg attcacctca
    27241 tacccatcca ccggttttcc tgtgaagtta tcccatgtgg cgtacttggc atcccaacgg
    27301 acgccatgca acatttcatc taaaaacaaa aaaccatttt ccatacttat cacctcttta
    27361 acgccaatgt aatcaggctg tcaatcagtg caggaagcgt gattcctgcg gcggccacca
    27421 tacgtgggta gcggctgtac gatgtaaaac cgggcatggt attgacctca tttagaacga
    27481 tgccgccatc ctcctgcaaa aaaagatcaa cacgggcaag ccctctgcat ccaagcaccc
    27541 gatatacttt ctttgccgtt tcctgcaccc gatttcgttc ctcgaccgga atgtctgcgg
    27601 gaactgtaat catcgcattt tctgagcctt tttccggctc gttttcctga tggatgcgga
    27661 agataccgtg gctcagccgg atttgatcca cttcgccgac aatcaaatca tcctcgttcc
    27721 ccatgaccgc acacccgacc tcacagcccg aaatcgcttg ctcaattaag atttttccat
    27781 catattgtcc tgccgcttct atcgcagcgt taagttcttc cgtaccgttt actttggtta
    27841 cgccaaagga cgaacctgac cgtgccggct tcacaaagac agggtaggta agcgcacccg
    27901 cctccggctt gtcaccttta tcaataattt gaaattcggg aacggcgatg cccgcatttt
    27961 ttgtaagaat gtaggccagt gatttgtcca tgcaagctgc ggagctttga atatcacagc
    28021 ccacataggg gataccagac aatacaaaca gcccctgtat cgcaccatcc tccccgaatt
    28081 tgccatgcaa aaccgggaaa gccacatcaa tacgccgtgt ttcgtattcg ctttctttca
    28141 tgacaagcag cccatgcgtt ttcctatccg gggagagtat ggcggggaga ctgtcggctt
    28201 cccattccgt acatggcttc ttgcatagct tccatacacc gttttttgta attccgatgt
    28261 agtgcggatc gaatttttcc gtatcaatgt tcgcagcaat ttctattgcg gattttaccg
    28321 acacatcatg ttcctccgag caaccgccga agatgattgc gacttttatt ctattcatgc
    28381 tgtaaactcc tttcaaaatt gagacaattc ctgattgttt tttctgtagt atcctgcagc
    28441 acacgctcag tgtagtacgc cgtatggggt gtgatgatca cattcggcat ccttaaaagc
    28501 tgcgacaaaa agggatggtc aagcactttc tgcgagcaat cggtataaac aaactgatcc
    28561 tcgccctcca acacatccag tgccgcacca cccagctttc cgctttccaa cgcctccacc
    28621 agcgccccgg tatcgacaag cgctccgcgc gcggtgttga ttaaaaatgc gccttgcttc
    28681 atctctccaa tttgcttctg accgatcaga tggcgggtat ccgcacaaag cggcacatgg
    28741 agcgtaacaa tatcgctgtt ttttagaagc tcatcaagct ggacataatc tgcatccatt
    28801 tttcggctgt tatcataggc cagcacatgg catccaaatc cccgcagcct tttgacgacc
    28861 gcttgcccga tatggccggt tccaataact cccacagtca tatcccccag ttctttcccc
    28921 cggatacgat ccaatctgaa atcttgttgc gccacggcgt gcatggtggg ttttgcaccc
    28981 cgtatggcca tcagcatcag catcaaagca taatccgcaa cgctgtccgg cgaatacgca
    29041 accgtgccaa ccgagattcc cattctctcg gcggcagtcg tatcaatgtg atcgcagccg
    29101 atgctgcggg tagaaatgta ttttaccccg acctttctca gcgcaagaat tgtcgcctcg
    29161 gaaacctcgg acttatggcc tacgctaacg cattgattgc cagcggccaa ttttgcgttg
    29221 tctgccgata tcgcatcgct aatcagcgta gggataatat gaaaatccgg cgataaagcg
    29281 cggaaagcat ttgcctcatc ctgttcgcat ccaaaaacag taatgcccac actttttctc
    29341 attggttctc ctcctgaata tccacacttt ccgggagggg ataacagatt ttgcatttgt
    29401 ttgtataaag cagcttgcaa tctattattt ccccggtacc taaggcaact tgcatccgtt
    29461 taacctgggc atattcataa atcccgccac tttcacggac atactggata ctgccatttg
    29521 taattttgta taatgcttgg ggctctttgt cggcggacac ctgaccgatg attttctcat
    29581 catctaaggt cacccatatt tggtaggtgc agtcggtatc gtttcgcact tttaaatcaa
    29641 tccagccctc tggaatggtt gcatccaccc ctttaatctc gtcactgttt ggctctggaa
    29701 actcctttac ttcgtgacct ctgcgctgga taattgtcaa tggcgtatgc aggaacatcc
    29761 aaaatagtaa attgctcatc tggcacatac cgccgcccga catggtggtg agtttaccat
    29821 tggtcaccgt aaggccgtct ttataggggg tatctttgtc cgcatggcgt accagccacc
    29881 agaaagaaaa ggtttccccc ggtcttatca ataagccgtt taaggtcttt gctgccagct
    29941 ttaagttgaa caccttgttt tcctggtata tcatgtcaaa tccggtattg tggttgtata
    30001 atgcgcaatc cgtttcaaag agcaaatggg aaagcgattt ttcccctatc gtctgcgcat
    30061 agcgacagcc gtcaaatctc attcccgcat aaaagcacat ttttctctgc gcccgtctca
    30121 ttggaagcag gaacgggaaa cgctgtgtca atctttttct gtccataaca taacatcctt
    30181 ttctcatttt tctggtttta aatattcctc aaggcaaacc ccttggttgt acatctctgt
    30241 ggcagcttcg atgccaacat atcggtaatg ccacggctcg ttgctcacac cggttatctc
    30301 tgtcttgtct ggtgggtagc gacgaataaa accaaaacga tagctgtttt catccagcca
    30361 tctataaacc tcgttgcctg ttgaatgaat tccgtccgca ttgatatcca cagcaagacc
    30421 aagctgatgc tcgctcgttc ccggcacggc cacccaagtt tctgcttccg ctttagcctg
    30481 tgcagaggtg tagcctttcg ccttatattc ggcaattttt tcatccataa tttcttgctg
    30541 tttttctgtt gttcggtatc cggatgcgac aatcgggtaa actccatcag ttcttgcggc
    30601 atcaaacaaa tcttgaagat agggagaaat ccgaatatct atccgctcac catttgatag
    30661 ttgctcaagc tccacatcgt actgtgcggg gatggggttc tgcctgttca ctaaaatcag
    30721 gctccattcc tgttcatctc cctgttcttt ggcggtggca agagcgtttt cctccaaatt
    30781 ctccactacg tcagtgctgc tgcctgtatg agttgcaggg atgggcggta tctgtgttgc
    30841 ctccaatacg gaagccagtc tccacctcaa aaacagcgtg cagactgtga acgaaataat
    30901 gaacgcacat agaaaagccc gcttttccac ggattgtttc atatgccgtt tgtgatgatc
    30961 cgcattggaa tgatagttgc ttttttccat ttgatcgaat gcctcctttg caataaaact
    31021 acgatttgtg gctggtacga ttgttattat ggcaagcgca tctgtacttt ccctgtagcc
    31081 agtctgtagt tttcctgtag cagcaaaaaa agaccggcag ggttatacct gtcggtcaaa
    31141 atcatttaag ttttaaatat ttacaatgtt gatgtgagcg gtaaatccag ccagaacaaa
    31201 acgccatccg aggtgttttc cagcgcatac tggaggctca tggcatccag cgttttttgt
    31261 acgatggcaa gtcctaaacc gcttcgccca ctttttctgc ttcgcgcctg atcaatgcga
    31321 tagaatgggg tgaacagcct tggaagcgca gtatcatcaa tgtgaacgcc catgttcaaa
    31381 acaaaaaggc ggcatttttc agcaccaggc tcactccata tccgtacctc gcctccctgc
    31441 ggcgtgttct gaactgcatt caatatgaca ttggatagtg ccttttggag cagcctcgga
    31501 tcggacagga caatttgccc ggctggaata tctgtgacga accgctggtt gtttgcctct
    31561 gccaaggttt gaaaatcggg cagcaactcg gcaaccgtgc gccctatgtc caacggttca
    31621 gctatgggta cgattctccc atcattcagg ctgacaagct ccagtatttc ggaaatgatt
    31681 ttgccctgcc tatccatcat tttgatgcat tcgcgcagat acttagaatg atctttgtag
    31741 tcaccgatat ttccaagcat tccctccaac agaacgcttg tagccgcgat gggcgttttt
    31801 agctcatgag aagctgccgc aaagaaatat cgctgtgttt cctccaactc atgttccctt
    31861 gcgatttcat cctccagcct tgcgatggtt tctttcagcc tgacatacat ggaatgcata
    31921 tcatgagcca gtgcgccaag ctcatccttt cgctccagcg gcggcggtac atctttcagg
    31981 tttgccattt tattcgcact gtccgctaag gctttgatcg gcgttgtcat ttgccgcgca
    32041 aagatatacg cgcataaaag gctgaataca accattatcg caatcataac aattccccga
    32101 attgtcagcc cttgataaag caatcccaca cctgccttgt tttgagcaac aatcgaaata
    32161 ttatcatctc tatgtaccac ataaagaaag tcgggtctaa aactatttga tgtattggca
    32221 tttggtgtgg catagagtac gcttccctct ttatcttcaa tataaaactc aaaggattgg
    32281 ttattgtagt gaaacagccc tgccacctct tgaatatcaa gcctatcgct attctgaatc
    32341 agttccacca atggctgata ggattttact gtttgctgta attccatcac tctgaaataa
    32401 gacacaaatt gctgtgcaaa cagtgttgct gttacaccga caaccagtag caggacaatg
    32461 atcgtatagg aaaaaacctt aatgaaaatc ccttttcttt ccattattat gattcctcca
    32521 atcggtatcc tacaccgcga atggttttga tgatattttc cggcagcttg gcccgcaaat
    32581 ttttgatatg agtgtggact gtgctgccat caccctcaaa gtcatatccc catatacgtg
    32641 acaaaatgat ttcatgcgtt aaggttctgc ctttgttctg caccagcagc aaaaggattt
    32701 caaattcttt tcgtgtcagg ggcagctctg taccgtcaca aagtaccgtg aaatcctccg
    32761 gcagaagtgt cagcctgccg acacggattt cctttgccag cgcaccgctg cgccgcagca
    32821 gggcttccac ccgctttagt aaaatctgca tcttgaatgg ctttgttaca tagtcgtctg
    32881 cctctgcatc aaacgcccgg atttggtttt cgtcatccga cagggctgtc atcatcagaa
    32941 tgggagtatc atttttcgca cgaaattcac gcaaaagttc atgcccgttc ataccgggca
    33001 gcataatatc aagaataacc agttgataag tgttttcgta aaacttggtg tatgcctcat
    33061 ttccatctgt gcaggcatcc acctgatatc ctgcctcagc cagaaacgcc cttactgtat
    33121 tacagatatg atcatcatcc tcgacaagta gaattcgtat cgacataaaa atccctccat
    33181 tttaagaaga taacataaca gtctgtagtt tccctgtagt ttcaaagcga tttcttttcg
    33241 gattttaatc atccgtcatt gggatactcg cttcttttca tggtgtatgt atctatatta
    33301 ttggtttaaa cgaattaaat caagataata ataattaaca ccttcttaat gtttgggatg
    33361 tttcgattac agcggttgtc aaaacatccc gataatactg gcaagcaatg gatcagaata
    33421 tcctttacgg atattctgat ccaccacttg cccgcctgcg gcgttctcgt tttgcttgtt
    33481 ggggacaccc caagcctcag caaaatgcgc ggcctctttt ttgcgaatgc accttgcggt
    33541 atggcttttc acacaggcag ggcctgtgtg tactccctcc ggtcgctctt gccgcactcc
    33601 gcgcagcaga ttcagtccta ataccgttca agacagtaga aaatcagcaa gtctatattc
    33661 ctcatatctt catcatccgt gtccttgcag ttacaggaaa tgtaataatc gccaaatgta
    33721 aatcccagaa tattttcctc aaaaagccgt acctccccgg tattaaaatc aatcacccca
    33781 tactcaaagg cgcattagac cagcagttat ccaatgtctg gagacttcac cataaagaat
    33841 ccagatgccg gccttttcaa ttctaccata tacggctgat acagcagcag cttcctcgtc
    33901 tttgtttcgg gcattttcca tttctgtcat gataaagttt cgcttcttca tacatccatg
    33961 tgtaaatctt ttctttctgt ttcagatgag aatacttttt atcggtctgg agcagcctgc
    34021 catctatcat cttatagttt ttccgtgaca aaataccatg ctttctctct cttcaaaacc
    34081 atatccaaaa tgcgccggtt tcattatact attcagtaac cgcctttcct ttctttgtac
    34141 gcctgtcaat cggcttttcc gcagttttgg gaatgagcca gatcgtcgcc atctttacag
    34201 cgccggggat gcgtcccgca gcacaatagt agtttatcca tcgtgggggc acaccccatt
    34261 tctcgcctgc ctctttcaac gtcatgtagt ccatagtaca cctccacagg gtacattata
    34321 attcttttgc tcgaagaata aaagaaacaa aatgtgaaat ataaacttta agaaaggtgc
    34381 atataccaac attccacggg cagagtatga aatatacaat gtaagtggga ggtgcgatat
    34441 ggcgaatatt gaagattgtc ccggctttga aactttcggc gcggatgtga aagcggcccg
    34501 gcaagcaaag cgcgtttcgc gcaaagcaat ggcagagaaa ataaatattg actggcgcta
    34561 tcttgccaac atcgaaaacg acggggccat tcccagtctg ccggttgtca tacagcttat
    34621 taaagcctgc gggcttcctg tggaacgcta ctttaacccg gagatcatgc gggaggaaag
    34681 cgaactgcgg caaagggtca gccacaagct gaaactctgc cccgaagaat acctgccgat
    34741 catcgagggc gcgattgaca gcgccatact aattgaaaag ccgaagaaga atgaaacgga
    34801 ggatgtgtaa ctggcatcct ctgttttact tttagcggga gctgcacagc gtagcttcta
    34861 tttttttgca cttttttctt tccagagggc tattttgcgg gtagtttggg gctttggaaa
    34921 cgtcttgtta gtggagaaac agtaacgtag caagcatagg gagtgtagcc actcccggaa
    34981 aaacggcctg ccccgtccct gccgggccgc gtccgttttg cttgttgggg aaatccccaa
    35041 acccctgaat ggaaaggacg gtgaagcatg gcaaacagaa agcgaaaaat cgtgctgcgc
    35101 gtccctgtga cgccggagga acgggctatg attgaacaga aaatggcgct gctgcatacg
    35161 aagaactttt cagcctacgc ccggaagatg ctcattgacg gctatatcgt caacatggac
    35221 accagcgaca tccgggcgca gaccgccgag atacagaaga tcggcgtcaa cgtcaatcag
    35281 attgcaaaga ggctgaacgg tatggggccg gtgtacgcac aggacatcga ggatataaag
    35341 ggggcgctgg cgcagatatg gcagttacaa agatacatcc tatcaagtca acgctgaaaa
    35401 aagcccttga ctacatcgag aacccggaca aaactgacgg caagctgctg gtggcctcct
    35461 ttgcctgctc ttatgaaaca gcggacattg aatttgaaat gctgctggcg caggcatacc
    35521 aaaagggcaa taatctgggc caccacctga tacaatcctt tgcccccggc gaagccacgc
    35581 cggaacaggc ccatgagatc gggaagcagc ttgccgagga agtgctgcaa gggaaatatc
    35641 cctatgtgct gactacccat attgataagg gccacgtcca caatcacatt attttctgtg
    35701 ccgtggatat ggtaaatcag cgcaagtaca tttccaacaa acagagctat gcctatatcc
    35761 ggcgcaccag cgacaggctg tgcaaggaaa acggattgtc cgtggtgaag cccggccaga
    35821 gtaagggcaa gagctatgcc gagtgggacg cccagcggaa agggacgagc tggaaagcga
    35881 agctcaaagc agccattgac gccgctatcc cgcaggcaaa agattttgat gatttcctgc
    35941 ggctcatgca ggcgcagggc tatgagatca agccgggaaa gtttatttct ttccgcgctc
    36001 ccggacagga acgctttacc cgctgcaaga cattggggga agcctacacc gaggaagcca
    36061 tcaaagagcg tatcaaaggc cgcgttatca ccagagcgcc caaagagcgc aaggggattt
    36121 ctttgcggat tgatctggaa aacaacatta aggcccagca gtcggcaggc tatgagaggt
    36181 gggcgaagct ccacaatttg aagcaggccg caaagacttt gaatttcctg actgaacatg
    36241 ggatagacac ctacccggat ttggaaagca aagtggccga gatcaccgcc gccagcgacg
    36301 aggccgccgc taccctcaaa gctatggaac accggcttgc ggatatggcg gtgctgataa
    36361 agaatatttc tacctacaag cagctctggc ccgtggctat ggaataccgc aatgcagcgg
    36421 ataaggcaaa attccggcgt gagcatgaaa gcacgttgat tttgtatgaa gctgcggcaa
    36481 aggccctaaa gggacagggt gtgaaaaagc tccccgatct gtacgccctg aaagccgagt
    36541 acaagcgatt ggccgaggaa aaggaacggc tgtatgagaa gtacggcgaa gcgaaaaagc
    36601 agatgcagga atacggcatc atcaagcaga atgtggacgg cattttgcgt atgacgccgg
    36661 gaaaggaatg gacgcaagag ctatgagtat tccacgaatg aattaccctc aatatcgcaa
    36721 ggcccggaag ctgacgcatg agtgctgcaa ctattgtgac ggcaactgcc tgttgctgga
    36781 cgacggcgaa gaatgtattt gcgtacagag catttcctat tcgcttctgt gccgctggtt
    36841 taaggccgct gtcctcccgc tggatgcggc cctgtatgcc gagatcacca aagaccggga
    36901 cgaagtgaaa cgctgctgtg agtgcggggc cacgttcacg ccgaagtcta accgggccaa
    36961 atactgcgcg gcctgctcca aacggatgcg ccgccgcaaa gaggccgagc gccagcggaa
    37021 aagatactgg aaacctacgc acttagagct gtaaaagccc ttatatttca aggcttttcc
    37081 ggcatggtca gcggggtatg tgggtgtttt atcctatccc cgccgaagtg ctgttctaaa
    37141 tgcgtagaat ttaccagcag aaaagcgccg ttttctctgg ttccatgctt ccgaatatgg
    37201 tatcatagaa aaaatcagtc tgacgggggt atcaggatgg acggacaaga aaagctgctg
    37261 gactatgaaa cgatcaaagc cgccgttgcc ggtgagaggt gggccacgga aaaagtgttg
    37321 gcccattatg ctgactacat caacgagctt tccacggtgg agatcagaca ggcaaacggc
    37381 aaaaagaaga aagtcattga cgaagatata cggcagcgca tttccctgaa actgctggaa
    37441 gcattgccga gcttcccgct ggaacagaga tagacggcaa gagggctgca ccctcttacg
    37501 ccgctttgcg gctatcacct gcacacccct gaaacagata cacccgctgc gcggctatat
    37561 ctgtttcagg ggtctaatta tagctgcaac ctacgcattt aagggattga aaaccgcccg
    37621 ctgcaaggct tctcccgcat gtttgcaggg gcaagcgaat acttcatcct atccccgaca
    37681 ataccgggtt ctaaatgcgt aggaataggc aaaaagaaaa gacgccggac gcaagcgacc
    37741 acatggccgc cgcgcccggc gtctgctgtt cgttattcgt ctgtgaagtg gaacttctgg
    37801 ccgtcatagt cggcatccgt catgcgttcc agcttggaaa gggtctgctg ggcctgcgcc
    37861 cgcatatccc catccataac cggcatgacg gcggtgagcc tgtttatcgt tctgcgccgg
    37921 tctgcattgt ggtacataca gatcaggttt tcttcctcca cggtaaagtg catcattcct
    37981 tatacctcca attcattctt tttggttctg gccgccgcct tttttgggct ggcggcctgt
    38041 ttgctgtcgg atagctgctt gcggatagag ggccggggcc tgcgaatact cacatcccgg
    38101 cgctcgtccc ggttaatcac ggcatacacg ccatgcctgc ctttcatccc ggtaaaacac
    38161 agggatttat agggcagcat ggcaaagagc cggtcattat ccttggtgga agcaatcgcc
    38221 gcaaagtgcg gggagatctc caccatgaaa tgcgttttat tggggctgtt aggcgcggac
    38281 agatctttca ggctgtccac aatacgccgg gcctcggttt caatccggct gtcatgcaaa
    38341 gcggtgacgt ggactttgaa atcgccgggg acggcccgcc cccggctgtg ctgctccgtc
    38401 cgtagcgcct cctgcagcac agccgggtca tggggctggg cctcatagta caaaaagttt
    38461 cccaataccc ggtcagggct tccgtcaaaa cgctggctct ccgggatgtc acgggtgcca
    38521 tgctcataaa agagccggtt cacactcacc ggctgggcgg tttcaatgac gtgccggaaa
    38581 tgctgcggat agtccagctc atagagattg ccccggatag tgccgccctg tgtgccggtc
    38641 agctccaccg cgtaggccag tatcttgtca cgggtctgct ccccgtaaaa tttccatgtg
    38701 ttgtactgtc gggtgtcttt caggaaaaca tcacgttccc ggaaacagta cgtcccggag
    38761 ggccgggaca tccagagcag ggtcttgtct gcggcctgat cgctggcggc atcccgcctg
    38821 ataatgtctt tgtcaatgcc aaagtcactt tgataaaacg cggtgttctg ccgcatgatc
    38881 tgttccagcg cggccagaac gtccacattt tcaaacttct gcatttactc tacctccaat
    38941 ccctttgatt tttcccgcgc cggtggctgc ttggcctgtt cggtcttggc cgcgttgagc
    39001 tgcgcccgga tagagggctt ttctttctcc ctgcgggccg caagctgtaa ggcgctgggc
    39061 ttttcgccgg aaaggggctg gatgcacttg atacggtcag cgacgcggat tgtggtgtcg
    39121 tttaactgcc gctgccatgc cccggccttg ggcgaccagc ggaaaccgtt gcttttcagc
    39181 tcgtcgcgta cagcagcatc cggcttcccc tcaaagaaga tttgcaggcg gttggccacc
    39241 cggttgatct ccacagtgcc gccgtcaaac tcccatccca cataggccgc ctccttttgg
    39301 cgggtcaatt cctcaatgcg gccctttaac cggcgtatct cggcgttatt gttggagagc
    39361 tgccaggact ggaacggttt tttctcatag tgccagcccg acgccatatc cgctttcagg
    39421 ttttcaatgc ctctttcggt cagatggggg catccgtcca gcgtcccatg cttgcggtag
    39481 taggcattga cggccttcat caactcctgc gacgcttcca gcttttccag tttcttttcc
    39541 agcttggaaa cggcctgcgg atcgtcggcg ctgataccac ccatgccggt gctgcggatt
    39601 ttgtcaagca agccctgaat gtactgaaat tcctccatgt tgcggtcact ggcggcgttc
    39661 tgcttttctt tcgcccgtac cgggaaattg gacggcccgg caatcaggac agaggggacg
    39721 cgggcggtaa tctcatagcc cttattcatg ttctcggcca gcttgcgggc gtaggtgtca
    39781 aggaggctgt ctatcttttc gtgatactct ggctccacac gctttttctg gcgctccgca
    39841 atttccacgg cctgatcgac atagtgccgg tattccgccg tggcgctgcc gggccggtag
    39901 tcagaaaagc tcatggcttc cttggcgcgg cgggcggctc cctcattgat gggataatag
    39961 cggacagccg tttgcggggg cgcagcctcc gggggttcct cctgtgtctg gccggaagca
    40021 actgccacat cctcaaaaga tagctgttcg ggttcctccg gctgcggttc gggcggcgta
    40081 ttgttgataa tgccgtcaat catgttttcg ttctgctccg tagacagttc ggcggtacgc
    40141 aggggatttt cccgcaaaaa ctccggcact tccatatagc cgaagctgtc tacatagtgg
    40201 gcggtgtcct gtccattctg gtgcagcacc accacgtcgg aaacggaaag gctgtgtccc
    40261 ctaaaatctg ccgggtggtc gatgttgaat gtgcggtaaa tatcctccaa agacgtgctt
    40321 ttatccagcg gtgccgtata aacaaggtca tagttggcac tgtccacggc cagccctgcg
    40381 gcctgtaaac gctcgtatgg ctcaaaccga tagtcgcggg tttcctcccc gcctcgaagc
    40441 tggtagatgg aaaaggtgtc ctgttggggt gcttcatagc cagccgccgc gtattcctcc
    40501 acggtcatac cggctgcggc ggcctcctgc gcgatctcgg cctgtacctg tgctttgtag
    40561 tcctgcaagc tctggtcgaa gtcagccagt ttcggcggct ccggcaactg atactggccc
    40621 tgattgagca tggggcggca ctcctgcaca taagagagaa gcgcccccaa ataggccgtt
    40681 tcctccggcg tcagggtttc cccggactgt aagcggccct gcgccgtctg ttccatttgg
    40741 gcaaggctgc aatgcagctt catatagggg acaaactcgc ctaacagcat atcccgatgg
    40801 gccttgtcct gctcccagcc ctctatgccg gttgtagcga ttacatgctt tttccagttt
    40861 tcgtcctgcg cgtaaaattg gtggtagctc tggatatgtt cgatcaggga accgtcccta
    40921 tctccaaaat cctgccgccc ctcgtagctg tccggctcgc cctgaaagat acagtcaatg
    40981 cggaacgcgg ttttgtcata gcctgcctct gccggatatg ctgcatccag acgggcaaac
    41041 agcgcgtcgg cctcatgcaa cggcatttgc tggccgtcct ttagctgggg gctttcgctc
    41101 catagcacag tcacaagcgg ctcggctgcc ggatcaaggg ccggggcctg ttcttcctcg
    41161 gcctgcgccg gggccgctgc ctccggggta acgtcaaccg tgacggcctc ctgttcctgc
    41221 cgggcctcgc gctcctgctg aagctgggca aaatggccgt cgatctcggt tatcaattcg
    41281 ctggccgtgg cccggatggt ttcaagggag gctttcagct cggataactc cctgccgctg
    41341 ctccaatcgg ccacatagcc aaaagaatag tccgacgtgt ccagcccgta atactggcag
    41401 acggtatagg ccacgctttc ggcctgtacc tcgcgggtgc tgcggtctgg ccgggccgcc
    41461 tgttcctcgg cgggggcctc ccggtcaatg gcgtggagct tggcatgggc aatctcatgg
    41521 atggcggtct ttagggtctg caattcgctc atgccctcgt tgatggcaat gcgctgtttt
    41581 gcgtagaagc agcggccctt ggccccgtct gaaatcggct caatgcttat gggatagggt
    41641 gatgtccgtt ccagcgcggc aaagaaatcc tgatactgct gcacgtcgcc ggtaagttca
    41701 ctgatatagg gcatgggaaa atccttgccc tcggtctggc tgatgtcaaa gactgtcacc
    41761 accttaaagg ccggaaccgt cacttcctgt tcttcggtca cgggcttgcc gtccgcgccg
    41821 atcacgggct gctgggtggc cgggtcgatt ttctccatct gctgcttaac cttgaacggg
    41881 gccggggcca gtatcttaat gcctttctcg ccccgcttca catggcggtc aaattcatcc
    41941 tgccacctgt tatagcccgc cacaatcgaa ccgccctgca tggcgatcag cagcgtattg
    42001 ttcagactgt aatcgtgcat cttggacata gcggataagt aagccatata gcggctactt
    42061 tcaaaaagct cctgtacgcc ggtttccaga cggtcagtaa tcactttcat gcgttctttg
    42121 ctgttcatgc cgtccagcat gatcgggata accgggggcg gcgctttgtc ggggctttcg
    42181 gatgcctccg gctgctgggc ctgcggttcc tcccggtcag cttccggctg ggggtagtgc
    42241 atcacccggt attcctccgg caccggctgg ccctcatgga cgcgctgcca ttcgtcgccg
    42301 cttctcacca gatagccgta ctgggtaaaa acgccttgtt cctgctgggc aatgtggcgt
    42361 ccaaactgcg ccgtatcaat cccggctttc cattcctccg gcatatccac catgccggaa
    42421 cggttcaggt agtagtcgcc caactgtcca tcccccctaa cgtcgggaag atgcacatag
    42481 taatccacat tttcggtgta gtcgatgatc tggccgatat tctcaaagcc gccacgggga
    42541 ttttgcaggg cggcgttcag ctctgcgatc tcggcggcgt caagctggcc cagccgggcg
    42601 gcaaggaaat tcagctcgtc cacgtcggcg gccagcacca tgtcataggg aatggcaagg
    42661 tgtctgtcct ccgggcagga atacccgttg ataaaaaaat cctgcggatt gtcggcagta
    42721 atgccaacat cccgcagggc ggcttgcagc gtttcggttg tggtgggcaa atccagccag
    42781 acgccttgtt tgccctgttc caaaaactgc cggttatgga cttggataga aaatacttcg
    42841 ttcacgcggt cactcctttc tctgttctgt ttcaaaaaat agggggttgg ggacttcccc
    42901 aacaagcaaa aatccccggc ccggcaggga cgcggccggg ggattttacc gggagtgtag
    42961 ccacactccc tatgcttgct gtgttactgt cctttagctc ctgtgttcaa tgcggtagtt
    43021 gacgtacttg cccccggtat cagccaaaag caccgtcccg tcgtaggttt tgccggtctt
    43081 tacggaatag aggcccttta ccttgacttt gccggttttg agcagcgcag cggcgatctt
    43141 cggggtgaac gctttcttgc gttcctcaaa aaatcggtca ttcttccaca tgacgaactg
    43201 gcaggcccgg tttccgcagt agtagttttt cttgccctca tagacagcct caccacagcg
    43261 gggacacgtc ccgataggaa cacgctcggc ctgaaacaat ttctgctttt cctcactgat
    43321 acaggaatag gtctgcacca gctcccgcgc ctgcgtctcg atgccgtcca taaattcgtc
    43381 aggatcggcc tgccccttgg cgatctcggt cagccggttt tcccattcgg cagtaagctg
    43441 gggagaagta agcgcctccg gcagcaccac agccagattg atgccgtcct tggtgggaat
    43501 gagctgcttg ccttttctct ggacaaagcc catctgcacc agcttttcca gaatggcggc
    43561 gcgggtggcc ggtgtgccta atcccttgcg ctcggcatcc tccggcatat cctcggcccc
    43621 ggcccgttcc atagcggaca aaagcgtatc ttcggtgtag ggcttgggcg gcgtggtgaa
    43681 atgctcggtg acagaggcgg ccacgtttgc aaaggtctgg ccctcgcaaa gctccggcag
    43741 ctcccgcacc gcctcggcgt cctcgtcggc gtcggtcttt aaggaagaac ggaaccggcg
    43801 ttcaatctct ttccagccgg gggaaaggac ggtcttgccc ttggcggtaa actcctgccc
    43861 ggcacaggta aatgtggcgg tgacggcctc atagatatgc ggcccggcca cggcacataa
    43921 gagcttgcag cagatcaggg acagcagctt tttttcactc tcggccagag tgccaaagcc
    43981 ctgcttcaca aattcagcgg tggggataat ggcgtggtgg tcggacacct ttttgctgtt
    44041 caggatgcgg tcaaactgcg gggaaaggtt caggcccttg gcaaacggca ccagctccca
    44101 caggccggac acaaggcttt ccacggcggg cagcatatcg tcggtaagat attgactatc
    44161 cgttctcgga taggtcagca gcttctttag atacagtgcc tgggcatagt caagggtctg
    44221 cttggcggta tagccgaaaa gccggttagc ctcccgctgc aaggtggtaa ggtcatagag
    44281 cttgggcggc tgttccgtct ttttctcccg cgtcacggaa acgcaaacgg cctgcgcttt
    44341 tgcacaagcc gccttgacgg tgtttgcctc ggcagcgtcg gaaaagcggc tgcttaccgc
    44401 gtccgcgccg cccgccgtga tatggacgat gtgatatttc tctttcttga aatcggtaat
    44461 cttggcgtcc ctgtccacca gcatttttaa ggtcggcgtc tggacgcggc ccacagtcag
    44521 gttcttgtgg tacaggacgg aaaaaagccg ggtggcgtta atgccgatca gccagtcggc
    44581 cttggcccgg cacagggccg attgatacag cggatcatag tcgccgcccg gtttcaggtg
    44641 ggcaaagccg tccttaatag cactgtcctc catagaggaa atccaaaggc gctcaaaggg
    44701 cttttggcat cccgccatct ggtagacgaa acggaaaatc aattccccct cgcgcccggc
    44761 gtcggtggcg cacacaaggc cggtcacgtc ggggcgctgc atcagcgaac agagaacgtc
    44821 aaactgctgt ttcttgtcgt cggggacgat gtactgccat gtttccggga gaatgggcaa
    44881 atcctcatag cgccattttt tgaaacgctc gtcatagcgt ccggcgtcgg caaaggacac
    44941 cagatgtccg atgcaccagc tcaccagata gccgttcccc tccaaatagc cgtcgtgttt
    45001 ctccgtggcc ccgatcacgc ccgcaatgct gcgggccacg ctgggttttt ccgcgattac
    45061 aagtttatat gtcattcctc gtcctcctgt tcactcacaa aatcagcttc cgggtcaaac
    45121 tcggtttcgg gttcctcgtc ctccaaatcc tcgtcctccc caaaatcata ctcgcccaaa
    45181 tcgtcgctgc cggtggtttc agccttgggc tttttgaact tgaaatagta aagggccgca
    45241 ccgccgcccg ccagcgccac cagcaggaac acgatcaggc cgcccgcgcc gcccttgggt
    45301 tcttcctccg gggcttcggg ttcctccggc tggggttcct gcggctccgg gccgctgcac
    45361 tcggtcaggt tggttttaca taccgggcaa ttcgtgttca cagccccggc cttgcatttc
    45421 gtggtacagg agcagacagc agcgggggtg acggtttccc cgtcctccat cagcgtcagc
    45481 agatcggctt catccacctg atttaagaaa tggacagttt cttttccctc gtcgtcccgg
    45541 tcaataatca gataaaacac attgccgttt ttggtttcca cggtgataaa ctgcttgcca
    45601 tttttcgcgg cgctgccgat gtcgtcaatc agtgacaggt tcccgtccgg ggacagggcg
    45661 gcgctggccc cgccgccatt gaacagttcc agcagagaag caatttcctc cgggtcaatg
    45721 gagagggaaa aaccgccctc gccggtagcc gctacttcct tttcgctgac ggtcatatag
    45781 tcggaatgga catagccgat gcgctccggc aagagaattt taatccagtc gccgtctgtg
    45841 ccgactacct caaccgtcgt cccattggga agctgggtga acgcggtctt gtcaagcccc
    45901 gcgccggtgc ggacattcag cctgccgccg ttggtggtga cggtgccggt ctgttcaggt
    45961 tcttttttgt cggtgccggt ggtgatctgg atgccgtcgt ccgtggcggc aatgctcacc
    46021 ttggaaccga acagcgcgga aaacaggtcg gtgatgtcct tgccggtcag agggccggta
    46081 tctttttcgg cctcggtttc tgtcccgtcc gtggcggtgc cggtttcgtc tgtttcaggc
    46141 tccaaaccgc ccgtagcggg ttcggttgta gcctccggca gcggggccgg tgccgggtca
    46201 ttgccgcctg caagggcggt aacgggaaag gccgtgacgc agagcatcac ggccagaagt
    46261 gcggggagaa tacgggaaat ccgtctggtc ttattcttca tgggcattgt cctcctgttc
    46321 atggtaaggg gttgcggcag gggcggcctg cattgcgccg ctgcgtaaga acgcggcaaa
    46381 ttcctccggc ttcatgttca tgccgcgcac aagctggatg atctgcaaat tctcctgttc
    46441 ggttttctgc gtttccagct cccgaagctg tttctgaaac tccgcgatct tagacttggt
    46501 tttctggatg tccttttcga tgcggtcaag tttggttgcc ataattgaaa gctcctttct
    46561 ggcgttaagg taaacgcccg taactgtaaa aatgggattg ccagtagttt gaagtaaggt
    46621 tcgtgtagga aatcgggttc ccgcaatgca gcatcacatt gttgcccaca tacaggccga
    46681 cgtgggacac gccgggggtg tcgtaggttc ccttgaaaaa tacaaggtcg ccgggcttgg
    46741 cctgcgaact ggaaacaggg gtgcagatgt tatataggcc ctgcgccccc agccgtccca
    46801 cgttccatcc actatgattg attacccacg aaatgaagcc gctgcaatca aagctggtgc
    46861 tgggagagct gccgccccac acatagggat aaccgatata tttttccgcc tctttgagca
    46921 tggcggcaaa ggtggcatcc tccaaagcct ccgggggtac gtcgtagtag gtcggcggcg
    46981 tcagggtgga ggcgttgggg tactggctgc cggaaaacag atcaggccgg tttcctagcg
    47041 tggacatata cagggaatag aaacttagcg tttcctcgtc catgatatag actggcacat
    47101 gggacaggtc gaagttttcc agcgtgacgt agcagatgta atagtcatac ggcacttggt
    47161 aggtgtctgt atgggtgttg ccctccgcgt ccgtccatgt atcggtttcg gtgcggtaac
    47221 gggtttctac cacgacgttt tctgtcagga tatattgctt gtcaaagagc atggacaaat
    47281 caccctgtac ctccccgatg gtaaatatcc cgtcatggag cgccgacaga atggaaatca
    47341 gcacataggg gtcatggctg atctcgtcaa ggtcaaaatg gtattcgtca tagtcatggg
    47401 tggcctcgta gttgtccaga tagtcttgca gctcggcttc cagctccgcg taggcgtcct
    47461 cggcggccaa catatcgcta tcctcggaaa agtaagagga cgccgtgatg ccgcttccgg
    47521 cgctgccgaa catggcggtg caggactgta agccgccgat gataagcagc atcaaaagcc
    47581 ccaacacaag gacggccagc gcccctttcc agtggcgggc cacaaaggag gccgcccgtt
    47641 cactttcttt ggctgcggcc ttggccgctt tcgtggaggc cccggctgtc ttggccgcgc
    47701 cctgtgccgc cgcgcctgcg gtctggcccg ctgtccgggc ggccttggcg tactggcgct
    47761 tgatctgctg tttctgccag aaccgggaaa tggggttgct ggccgcctgt gcgatctccg
    47821 ggttatcccg caaagatttc tggtagaaat actccgcgtt ggcagacaat agcctgcgct
    47881 cggccttttc cgcgtcccag tagggtttca gcctgtggtg ccggatggtg ctgcggatgg
    47941 cttttccggc ctgccgctcg gcaagttcct cccccttatg gccgccctcc acacccacgt
    48001 tttcatgctc gacttcatgg attttcccgt ggccgaatag aaaaacctcg gaaacaggac
    48061 ggcctgccgg attgggtttc agggagggag gccccttgtc ctgtttgtca aagtgcagga
    48121 cggtctttgc tttgccgctt gcctcgtcaa agacggtttc cttgaccggg acgcgcttct
    48181 tggggatggc ggcgcgggcc ttatccagct tatcggcccg tttgtccgat tttcggatat
    48241 agggctgtaa atccgggtcg gcccgttctt cctccgacag ttggagccgg gcggatggcc
    48301 ggtgcagggc agcgtccccc tcggcggcca cagccccggc ctgcttcgcg gctttcttgg
    48361 ccttatggcc gtcatggatg gcgtcggccc gttccagcag cttatcggcg gtgccgcccg
    48421 gccccggcgc ggacaaatcc tgttcggcct cccggctgga aatgttctcc acctcgccgg
    48481 tggcctgatt ttccagcgtc agcccgtcgc gggtcatggc ctgcgtcact ttcgccttgg
    48541 ctttcagctc tttcatggtg gttcacctcc tttgtggcgt cgccgggtca tactcccgcc
    48601 acctcgttta agcgggtcgt cataatcttg taaagctggg tgttgtgcgg gatggggttg
    48661 gtaaagggca gcaccacgtt ttcaaagatc aaaaggccct cgccgggttc ggaattgtcg
    48721 atgtacttct gctgttcggt ggacaaattc aagcgttccg caaggatttt tctatccccg
    48781 gacgcctgat tgagcagcgt aatgaagtcg ctgttttcaa ggatattttc aatttccggg
    48841 gaggaaagca ggtctttgac gttctgggtg gcacctgttg gaatgccgcc ccatttccta
    48901 aaccgcttcc aaatctccgc agaataggcc gccgtctgtt cctctttcaa aagcaaatgg
    48961 aactcgtcgg caaaatacca tgtggccttt ccttggcttc ggttggccgt gacgcgcccc
    49021 catatctggt cttggacaat gagcatcccc agctttttga gctgcttgcc cagctcctta
    49081 atgtcaaagg acacaagccg gttgccgatg tccacgttgg tacggtggtt aaatacgttc
    49141 agggagccgg acacatacag gtcaagggcc tgcgccaccc ggtcagcctc cgggacatgc
    49201 tgatctaaca acgctttgtg gaggtcggac aggatcggca tattctccgg gcgggggtct
    49261 gccagatagg gccggtagat cacgcgcaca gcccggtcta tcacggtctt ttcaatggct
    49321 tccagcccgt tcttgccgcc catgacaagc tcacagaacg acagcacaaa gtcggatttc
    49381 agggccagcg ggttatcgtc ctccgaataa ttcaggttga tgtccagcgg gttcacaaag
    49441 tccttgctgg taggcgacag ccggattacc tgtccgtgga gccgtttgac aagggggtaa
    49501 tattcggcct ccgggtctga aataaaaata tcgtcaggcg tcgttaagaa cacactgacg
    49561 atctcggatt tacagctcat ggatttgccg ctgccggggg tgccgagctt caaggcattg
    49621 gggcaacggg cctgcttcct gtccagcata atcatgttgc gggacttggc gttgatgccg
    49681 taatacatgg ccgcgccgcc ctgaaaaagc tcctgcgtca caaaggggac gaacacggcc
    49741 acgctggaag tcgtaaggct gcgctgtatc ttgatctgat tgacgcccag cggcaggctg
    49801 cttgcaaggc cctgttcctg ctggtagtcc agccggacaa ggggacagtt atgcttttgg
    49861 gccacgcctg cggcttggaa cacgtcgtta ttcagctttt gcttggtgtc ggccacgttg
    49921 aggacaagga acgtcaactg gaacagccgt tcattgcggg tctgcaactt tgtcagcagc
    49981 ttcttggcgt cctcgccgta ggtggcaagg tcgctgggaa gaatgtccat atcgtaaccg
    50041 ctgcggacgg cctttttctg ctccgcaatc ttcatagcgt caaggtcggt aattttgcgc
    50101 ttgatggtct tgatcgcctt ggtctgttca atggcctgca catggagatt gactaaaaca
    50161 ccgctttccg tattgaggaa gtcggccagc atatcgtccg acagctccgg ggacaggatt
    50221 tgcaggaaac ttaccgcgcc gtatttgccg cccatgccga atgtctttgc attgccgaag
    50281 cacagggagg acggggcaat aaaatccttg gtggagaggc cggacggggc cagccacttc
    50341 cagtcaaaat tgaatatctc gccgtccggg tggtacacgc catgcagcag gttcagccgt
    50401 tcccagccgt ccatcacatg ggctacggcc cccatgctct tgaaatagcc taacaggtca
    50461 aagccgatgc gcttcaatct ggctcgcgcc gctttcacgc tttcggcctc gatggtgaat
    50521 gtcaggtatt tggtctttac catgccgttg ttgccgtttg caagctgctt ttgcagaatg
    50581 ccggtgtact cggcccggat gtcgtcgaag tcgtccccct gcggcgcgat ctcaaagctc
    50641 ttggcgtact gcaccgggtc aactttccga ttgaggaaag aaaactggat atgaatggag
    50701 gcgtccacat agttatacag gtcgcacagt ttttcaaaga tagccgtctg cgtgtccggc
    50761 tgggcaagct gatagctgat gtccgcaaac tcaatgcact tggaaaagac gccgggcgat
    50821 acccggcaca cgccgtccgg gtacatgacc tcgtagggga tgctctgctg cgccgtgtgg
    50881 ggcctgccgt cgcccttata tttacggatt accgcgtcaa tttcttttcg ctgggcgcgg
    50941 gtcagctttg ttttttcggt ttctcttggg atttcctttt tggacaatgg cctttacctc
    51001 ctgttccatc tggtattgcc gcacaagggc ggtgtaggtg ttgtcggtct gatagggacg
    51061 ctcctttggc tggatgaagc agcactggat gatctgcata atcaccactt caaggggctg
    51121 gccgtgacgc tcgtacatgg caagcaaaaa gcccggcagc atggcaaata ccatgagcag
    51181 ggccgccgcg ctgttggtaa gatagtcacg ggtgagaaaa tataagggga cgccgatcac
    51241 cagcgccccg gaaaaacaga tcagttgccg tttggtaagc ccaaacacaa ccttgctttt
    51301 cacatgggtt aagtccttgg ggacggttac ataagccatt tggcacctcc tttcacgtca
    51361 ggataaggtc acattccacc tcgttccccc acacgtccca gccgggggtt ttctgtctgg
    51421 caaacagctc cacgcggggc aaatcgcccg ccagctctac gattttttca cgggtcacat
    51481 ccggcttttt gctgtgttgt tcaatcgggc tgataataaa ctgatggaca gaggcagaac
    51541 gacgcttcgg cttgccgtgt gttgccagca ggcagatttc cgcgtttccc cttgtccagc
    51601 ggcccagccc gtaaaaccat gtaaggcttt tacggttctg tttcagccat acaaaagcca
    51661 cggtcttgaa tgaaaagccc cacgctttga ttaaccgcag agcttccggc agcatgggga
    51721 atgtggccca caaaaacagc aggcagtctt tggacgccag ccgttccacc ggcagggcgc
    51781 acagctcgtc aatgctcatg gtggaatagt gattttccgc tgcgccctgt acgtttttgc
    51841 agtcataccg ccacggcggg tcagcgtaaa tgatgttgta cgttgaaata aacaagttcc
    51901 tcctgtgtat gcttggattt tgggatcaat gcgccccgaa tacggccttt gatatactgc
    51961 cggtcttgaa caggcagaag cacagcagca ccgtatagcc catacagccc cagattgcgc
    52021 cggatatgtc ggaagcggaa ccgatattct gtatcagcac cgcgtagata cccaccacaa
    52081 ccatgattaa aaaggcttgg aagcccagcg ccagcaggga tttcagatac ccttggccca
    52141 tgccgcgcca ttccgcgttg cccattgtgg caagggggat aggccccagc gacgtgacaa
    52201 catagatttc caccatgcgg ccataggtca caaggaaaat gcagatggaa aggatattca
    52261 tggtgaggcc cacaacaagg gtttggaacc acagccccaa aagagcgccc acgctcatag
    52321 cctccaattg gctttcaagg tctggtatca gggtgttaaa atcaatggat gtctgcccga
    52381 taatcacacc ggcgctttga ttgacgacgc tttgggtggc gtcgaatacg cccatgacga
    52441 tgttccatgt gttcgttacg atcaggattg caaacgcgga tttgaatatc cagcggaaga
    52501 acatggagct gtcaaagtcg tgcatattgt tcttttccgt tatcatctgg ataagctcat
    52561 agcacatgac aagggcgagt atggccccgg ctatggggac gatcacggtt tccgacaggc
    52621 tgtgcagcat attgaaaata ccgctgttcc atgcctgcgg ggttcgtccc acgtcggcgg
    52681 cgatttctcc gactttggca tttaccgtat cgaacagccc ggacaggttg cccgtgatac
    52741 cgtccatgag gatgcctttt atccattctt caatgatttc actaatcacg ggtagcacct
    52801 cctttcccgc gccctcccgg tctggccggg aggacgcgga ggcgattagt taagggtttg
    52861 gacagggaca aaggccagga tcagcccagc agcccggaca gcaggggaac cagagtgaca
    52921 ccgatcaggg ccacgccgcc gccagcgaca agctgtttta tgtcttttat gagaaaatat
    52981 ttgctcgaaa tgagccttga aaagattaaa atagattaaa ttttgataga tttttcttat
    53041 ttatgggagc cagaacctct ttcgatggtt ccaagctccg gtattggttt taagttgaaa
    53101 tgaatttcca ttgtgaccgt ttcaggagta cggcagtaat tttctgttcc gtctttctcc
    53161 tgcggttctg agataacgat tttactgatc agccggttca gaagattggc atccagttct
    53221 ttgatgtccg catactcgct aatatcgtca atccactgct gggactgtgc atccagtttt
    53281 tcttccgtat tcagcacact ctcattctgg aaaagttcct ttttgatatc tgtctgctcc
    53341 ttctgtgtct tctccagcat cttatcaaaa acagattctg taatcttgtc attgataagg
    53401 tcatcataca gttttgcaat cagccggtct aacgtttcca ggcggtcttt ctgctttgat
    53461 accagcttct ccagactttc cctgtgtccc tgtgtatctt cctcgcataa ctcctttacc
    53521 cggttttcca gttctgttcc gtctccggta actgcttttg cacattcctg gattctggta
    53581 aggactgcat catacaggtc atcagcgtcc acccggtgct gggtacaatg tgctttcccc
    53641 ttatggatat aagtggaaca ggtataaact tcatgctggt ctttggtgtg cgtcttcttc
    53701 aacgtcaacg caccgccaca ctctgcacag cggagtaatc cggcaaacat gctgtattct
    53761 ccctgttctg tctggcgttt cctgcctttc atctttgcct gtaccagatc aaatacatcc
    53821 tgcggaatga ttgcttcgtg ggtatccctg actgtgatcc agtcctccgg agctttatct
    53881 ccctgattgc caatcttgaa acggtagtac cttttctggg aagccatgtc accacagtaa
    53941 acgggattca tcagcaggga tttcagatag gattcatccc atacatattt cccattttcc
    54001 gggtctttct tttcccattt tgtgtaatag gtgcggagtc cccgtttccg gtgccaccat
    54061 gacgggcagg gaatctgctg gcgttccagt tctctggata taaagttaat cccatgaccg
    54121 tccaccgccc actggaaaat tttccggaca atcggtgctg tctcgtcgtc aatcaggaga
    54181 tgtccctttt cctccgggtc tttctgatat cccagtggtg gaacaacacc tgtaaacttt
    54241 cccttggttg cctgcaggtg ataggacgca tggactttct tggaaatatc tctggaatac
    54301 atctcgttca gcacgttctt aaatggggca atctcattgt ctccggcaaa ggtatccaca
    54361 ttatcatata atgcgatata ccttactcca tgctttggga aaaactcctc catcagaaat
    54421 cctgtctgta catggtttcg tcccagtcgg ctctggtctt tggtaattac aagattcacc
    54481 agtccttttt cacaggcttt gagaagtctc tgcaaatcag gtcggtctgt gttcaatcct
    54541 gtataaccat cgtcctgaaa cacatccacc acctgccagc cctgtgactg acagtaattc
    54601 accagtatgt ctctctggtt ggagatgctg gcactctctc cggtcaattc gtcatctttg
    54661 gaaagtcggc agtatacacc aactctccag gttgcatttc tttctatatt gttactcata
    54721 tatctgttgt tcatctgata cctccgttac tttgcacatc ctacgcagat ttcagattac
    54781 tgaactcata atcattatac tgcttatctt tcattattgc aagtttatct tttgttttcg
    54841 tgtcacgtat tttctgtgcg atcacctctg caaacacttc ttttgcgtcc tgatcaccgt
    54901 caaaatatac tctgatgttg ataattttct tcttcactgc cattgtaaaa cctccatttt
    54961 ttgcaaaaaa agagcagcta atccattttt acagattaac tgcccagact gttggttaca
    55021 tatattaaaa tttttgtatt aacatttcag aaattgggga tataataaag acagggataa
    55081 atccctgtca gatgaactgc atattttaat tttcccattg aaaacaagag gaatgtgtgg
    55141 tacatttgtt ataggaaaag cacccactcc aaagtggatg ccgcctgagt tgactaaatg
    55201 gtcttacgac caccgcctat cctgttggca gacaggatag gcatttttat ttcctcttgt
    55261 tcttccgatc aagaaaggca ataatcgcaa ttaacaggct accaaaagcg atcagtaaac
    55321 tgagtacgcc taagaatatt gagataatct cataagctgt catatcacgc acctcctttc
    55381 tttcatctca gaaccccgaa aggctccggt ctgtaaatat caggaggcag ccacaccctg
    55441 tcacaggtgc ttccccgtaa cattgctgtt accggataat cataccatta tatccgccat
    55501 attacaattc atttttgttc cagattcaca gcaatcctta ttctgtatgg atacctgcta
    55561 tttccgtttg attgccttgc accggcagac ggcagacggc agatagggta tctataaagc
    55621 ctgccgttca ctccaaaatt ctgccgtcac ccattctccc ttgttttttt aatcgaatgg
    55681 tattttcctg cttttcattc atccgataat ccagttcttc tttcatccgg cttctggctt
    55741 ctcgcatggt tctctgggag attccctgtg cttttgcctt ttcatccagt tcccggattg
    55801 tgactgcatt tccgtctgca agcagttcat agatcagttt cgttccccgt tgcagttttg
    55861 tttctgtcgg ctttccttcc tttccgtcca gaagatcatc tgcggaaatc tcataagctc
    55921 ccacccagcg gaatccttct tcatctccaa gtttaaatgc catcgtctca cctggaggtg
    55981 caagagaact tttctcatgg atcagtactc ttgttgtcgg gtctttcttt actttcccga
    56041 taaaaagcag gcttctcacc gccgccatga tatcaataga accaagtccc cggtaagtac
    56101 tctgtgtccc tgaagattta ttcagatgtc cgatcagcac aatcgcacaa ccggtctttt
    56161 cagcgatcat tcccagtttc cggaataccg gacggacttc atttgcccgg ttcatatcca
    56221 catcggcacc gataaaagcc tgtaccggat caatgattaa aagtctgacc tgattctgac
    56281 ggattgcttt ttcaatacgg tcatcggata acgtcaatgc ttcctctgta tcatcaatga
    56341 ccatcacccg actcaggtct gctcctgctt ccaccagcct cggcttaatc gtatctccca
    56401 taccgtcttc tgcagtctga tagatcacat taaaaggttc aatctcttcc atgttcggga
    56461 aagtcttacg gttcgtgcag gctgcggtca gcatcatggc aaaataagtt tttccctctc
    56521 ctggattgcc ctgaataatc gtcagttttc caaatggcag atagggaaac cacagccatt
    56581 ctacactggt ctgttcaatg tcttcataac acaacatcgg caccagttct tccttctccg
    56641 gtactttcat tgtaactgtt tcaatgattg atttcttcct gtctgcattt ttatcacaga
    56701 ggatttcatt ccagtccttt cgggacggtt tcagacggat gacactgtat ccgtccggga
    56761 tttctattgc caatttctca ctggcttcat tccctgcatg gtcattatcc aggcacagga
    56821 aaaccgaagt aatctgaggg cgttcagaaa gaaaagccat cagtgccgca ctggaaatgc
    56881 cacccagact caggtagctc cgctttttcc agtctttggg aaataattgg ataaaagaaa
    56941 gcagatcaat ggctgcttca aacacaaaca actgattatc cgtccctctg tgacagaatc
    57001 cgtaagattt atcagattca gccacatctc cccggtattt tgtttcacca gttcccctgc
    57061 aatgggcata tcttggaata ccgtctgcat ctctgccaac aaacaccaca ttatggtgct
    57121 tcttttcttc ataaatatcc ccactgccga tccattcctc caccatattc ttttcaatcc
    57181 ctctgttttc tgtcagatat ctgatgattc tatcattatt ttcctctttc tctggtaatc
    57241 ggaagtcagg ggacggggcg gggcaggtac tgttcctgcc ttcgccttcc tccccggtta
    57301 gcatcttgac agcttccgga aatgtggcat tgtaaaattc catcacaaaa tccaccggat
    57361 agccacccct cccctgactg tgcctgtacc accggtttcc ggataccgtt acactgtcat
    57421 gctttttcca acggtattcc tttccgcttt tccctagttg ttctccctgt gcgctcagaa
    57481 aagaaacaag atctgtctga tttgctctta taatttgttc ttctgtatac tgcattttgc
    57541 tcatcctttc ccgtctgtcc tgctgacaga ctgttataaa ctcatgtcat agtttcgctt
    57601 tttcttcact gcctgttgtt ttgcatcacg ctctgcacac tctttctttt tttcatccag
    57661 ttttctcaga agagaggtct tcttttctgg attctgttcc gactggttca acggctgttg
    57721 ctctgctttc ccgttttctt caagaatctc tttcgcctga actgtgtaaa atgtcgggaa
    57781 cttcacctgc ttctcccttt tttcttctga acgcccggaa gtatctgctt ctgccatttc
    57841 ccggtcacgt ctggcaatat ccgcacagct ctgtaccttt ttcacaaaag ttagttcctc
    57901 ctggtacggc tgattatctg ctttcagctc ttcgatcccc gcctgaagtt cttctttctc
    57961 ccgtttccat ctggcagttg gcacttttcc atttttatcc agatgttgtt tcagttcccg
    58021 ttcacatttg cggtaataat tgatttcttt tttatgctgc tgataatatc tctcccgtct
    58081 tttctgaaaa tagaaatggt tatactcatc aatcaatggc tgatttgttt ttattacctc
    58141 tgccatcttt tccagattct caagttcctt taactgtttc cgcttttcag aaatttcttt
    58201 tttactgaca gaaacaacac cgttcagttc ctcaatccgt tcctgcaagt catccatcgt
    58261 ttctatctga tgtgtctgaa gataactgat ggattttgca aactcctgta aattggagag
    58321 attcttacct ctctgtttca tctccgcata aaaattggga agtggtggtc tgccctgtgt
    58381 ctttttatca taataatcct gcaggctttc cagcagtgtc gggttctgcc tgcgatccag
    58441 ctctgctttc atttcctcat aagctgtctt catccactga atggattctt tcagagaaat
    58501 aaacatctgg ttaaactgcc ggattgcccg gttcaattct ccgatcaccg ttttgattcc
    58561 ctttttctcc attgcccgga cttcatatcc ctcatggatg gtcgggatca ggtcaatccc
    58621 ctgttctttg taggaacggt ggtcgattct ggatgccatg tggcactccc tgaatttccc
    58681 atttactttc tctgtccatg cccgccgcca ttcttttaaa agctccggat cattccatcc
    58741 ggtagtagaa acagcattga acatatcttt tcctttggca tccttcaccc ggtttccatc
    58801 ctcatcaaga atatattccc ttctctgctt attgccccag attccttcct ctgtaaaagg
    58861 acggattggg acaagcacat ggaaatgggg attatccggt tcttcttcat tttccgcttt
    58921 tccctcatgg actgccagat ccacgatcat gccacgtgcc acaaactgct cctggcagaa
    58981 ctcttttgcc agtctgatat tttcctcaag tgtcaattca ttctgcagtg caatgtcaaa
    59041 cgaataggca agctgtgcct tgttgttccg ttccacatgc tccacctcgt tccagagtgt
    59101 ttccctgtca gtcagacgct ctggcacata ctccggtgtc aggatttctg taaatatcac
    59161 acctgacttg ctcgtataat cgtgtatctt gttgtaataa ctgtcaaaaa tcctctgccc
    59221 ggaaatatag gctgcggaag cgaccaccat ctgccctttc cctctggata cattccttac
    59281 tgaaaaatga tacagtgcca ttaactgtca tctcccttct ctgtcttttt tactgcttct
    59341 gcaatctgct gttgatcttt gtgtaaggcg gctatatgag cctgccgcac aatactcttg
    59401 ttgtaactgc tgttctgcag ttgattcatc atgtcctgga cgtcttcctg ttccagatct
    59461 ctggacagcg gaaatacttt ctcaaactct gcccctttga caatcagccg gtgggaacgt
    59521 tccctgcgtt ctaccagctt cttccgctgc tccagaatgt ttttgtggtg aattgctgcc
    59581 tccagttcct gtctgctctt ttccatgttg gccttcagtt cctccaatga catggattca
    59641 tactttggat tttttgctct gctcattttc ttgtactcct tcttttttct gtaaacaaaa
    59701 agaaccggct tattcatgaa tgccgattcc tgggtataaa aaactgaggt cgttgtcctg
    59761 tgattaggaa atgctgacct cagcgttttt gaaatattta attgttgctc cttggaatga
    59821 aagtctttgt caccattgtc gtataaattt aaactcacat cccatatcag gatttcttta
    59881 attcttccat atacagttca gctatatatg ccggaccagt gcaccacagc tttgtcttct
    59941 catcatacag tgcttttcct gtctttgaac acaaaaaatc tgtcaatacc tcgtttctgt
    60001 ccttgcctgt ctcttctgcg atttcatctg tcaccatagt gatcagaagg tcgattgtat
    60061 aattcatttt ttcctttgga attgttactt cagccatatc ttctcacctt tcacaaattt
    60121 cagacactca atagctgcct cagttttaaa gcagtattga tctttcaatt tattcggcag
    60181 caactgtctg atacagaaat cgtctgctac tttatcacct gcagtcccaa aagcacccgc
    60241 aagataagcg gtaagagtag cgtttgttgc atcatccgca atctttcctg caatgatatc
    60301 atattttgcc atttcacgtt caacctcaat aaacattttt ttctttcggt gagcggcgat
    60361 acaatgtaac cagtccacat ctgcattttc aaaaatatgc gtttcaagat tttctgaaag
    60421 tttgaattca aaagttgaaa tatatccaaa cttctgcccc tcttcaattg ttccggttgc
    60481 gatggctttg gctattgagg ttctcagaaa actttctgcc tgttcctttg atgtggttaa
    60541 ataaaatccc tgtccaaaat cttttctctt tgcacatttg gcaagatctg gctcttttac
    60601 ttcacaataa cttccatgat agagaacaaa tccatctttt aactcaagca ctaatctcta
    60661 tcccctttct ttgcaaaaat tcagtaatat cttcaagcac agcttcatcg ccttcacaat
    60721 gaaaaatccc atagcatttt tctatatatc caagaacatc ggcttcttta aacaagtgga
    60781 taatctgttc aacaggtaaa tgccactctt ctgaagcaag ccggattagt cttgtttgca
    60841 taaaagtaat ctctgttttc tcactcatta tgaatcctcc tcattttctt agtcagtaaa
    60901 accggaattg ttgattacca aggcatattt ccaaatcgac taataaaagt tactgttagt
    60961 ccggttttat caatcattgc aagttttacg attgcgcctg tactgtttgc tttcctccgt
    61021 cgacattcaa atctgttgcc gtggcacctg gatcagcgca gttcacctct tatgtcttgg
    61081 agtccttttg catacttcca aaagaaccca gtccgcttga aacatttacg ataatagttt
    61141 tattttttct ctcattgtca tacaatgcac ctacttcccc agaatttatt ttaccacgta
    61201 ttcttctgtt cgtctgtcac ctgcatcgta tctgcattta ccagataaaa atctaccaat
    61261 tcggtctcgt aattgccatc cggatatact ttttctaaca ccagttcttc gcaggaatta
    61321 ccatctgcat ctgtcttgga accattatcg tacagacagt atctcacatt taccgtatta
    61381 ccgttctttg tttcctgcgt ttcgctgacg acagcataga cttctccctt tgcattcgcc
    61441 gtgtaactga acgccatatt ttgaagtgta ttctgctcct gaaggtaatt ataaactgcc
    61501 agcattgcat tttgaattgt cagaccatca tgctgccctt ctgtctcgta atcttctatg
    61561 gtatttccac tctgaatatc ctctgaatct gttgaattct ctgtatccaa atccggtaat
    61621 tctgtagtat cctcttcttg attatcttct tcatctattg tatccgaaga ccaccacaga
    61681 ccatccatac tctgcataac aaatggatta ttggttctgt atctccgttc accaccataa
    61741 tcttcctgca cagttatata cgtatatttg ctgtcacaac cagtcaccgt ttctgttcca
    61801 tccattccga acactacact cagaccactc attccaataa tcaaaagtgc aaatatcgca
    61861 gcagctccat agacaattat caatgccact ttgtttttac cgaattgtat ggataatgcc
    61921 tggcaattgt gtctgtataa actcacgatc ccccagatca aaagaaccgc cagactgatt
    61981 gtaataacag taatcgatat ttctgcgaaa cgatatctca cttcatatcc catatcacgt
    62041 tttataccat accacaatcc atagaagaag gtactatacg caagaaccaa agtaatgata
    62101 ctgatccgta tcggatgctc ttttttatcc ggcactgcct tcttcagtgc ctctgccatc
    62161 tgcgctgccg ctctctgctc tatccacata gcaagctctg cttttgacgg atatgtgcat
    62221 ttagtatccg gaatccctgc gtaacacatc tggctctctt gaatccctgt cgcatctcct
    62281 gctgccattg ctgtaaccac tttcaggtaa taatacaact tcaactgtgc tggattgaat
    62341 ttatctatac gaatatctgc cagctcctgt gcagcccgtt cataattgtt aaggagtaca
    62401 cccatccgtg ccataaccaa aagcatctga ttcgctacag cagggtaacg gtgatattgc
    62461 ttctcatata aataccgtgc atactcatgt cgttcttccg gtgttaaccg ataggacttc
    62521 tttctattct gtgccagcat cgcaattcgc agaccataag ccaattccaa aattgcaatc
    62581 agctggcaga taaatgcacc aaatccccag atatccgttg tataccgcac ataggtcacc
    62641 aacacaatgc ctagcattgc cggaatcacg atcaaccatt ttgtccaacc tgatcttctc
    62701 ataaaaatct cctgaattgt ttttctgaat atagtatatt accgcacaca ccaatcgacc
    62761 atatacatat tgtgacgatg ctgttactta ccataacagt tgctgtttct aaggtcatct
    62821 atttatagcc tgagaaataa ctggtttccc atctctgtcc aacaatggag tcagtccacc
    62881 tgaataccca catgcatgat ataaataatt cacaccagtt tccttatcca cccatatctc
    62941 agtagtagtt aatgctccct gagaatatat tttttcaaat ctctcttctt tcgccatttt
    63001 caatttcctc ccataatcaa tttttcggtt ttataaaaaa tgtttctctg ttactttctc
    63061 gattgtatca tgacatacag ttccatcttc ggcaatcgtc gattttctga taaatctcga
    63121 atgatgcttt aatactccat aagcatacca gtcatggttg caaaatactt tgcagaactc
    63181 tgcatagcca tgttccttac aggtatcgaa atacagacac ctgctgattt cataagtaaa
    63241 tccactatca tcatcctgca aaatttctgt ctcccaacat accttttttt cgccctccag
    63301 atcatctacc agagactgtc tcacaatctt atagccattc ggcaataaat cgacatgctt
    63361 ttttctcttt ttccgttacc atattttctc ccaacttgca cactaagtca taattttcca
    63421 aacactaaaa cttgaatttc tatttctgct gccatgccat tacatagtct ttttctccag
    63481 tagcttcatc caaaccgcta tactgaatct caaatccttc tctttgataa aaggatattg
    63541 cccgtgtatt tttttgatat acatttaaga ttaatttgtt tctttttccc tttacatagt
    63601 ttagcaaaat tttacctata ccttgagatt gcatttcggc agaaacaaaa ataccttcaa
    63661 tgtattcgtt acttaaccct ataaaaccct gtatttcctg tttatcctcg taaacataga
    63721 cagttgcctg taatagaagt tcttttacta actcaaaatt acttttccaa tattgagcag
    63781 agataaaata atgtgtcttt atatttgtat ctaaccatat ttctgcaact ttatttatat
    63841 ccactttccg taattctcta atcataacgg tgatgctcct caaactccga ttttctcaat
    63901 ttttcaaact ggaatttacg aactacaata tcttttcgag aaaaagatgg tttttgtgtg
    63961 caattacatc tccaactttt ttagaagcac tagcaattat ttcaaatcca tttttttgat
    64021 acaaagatag cgcaggaata ttttgacttc ctgtatccaa acgtagtgcc ctgcaactat
    64081 ttttttgtgc taattctgta gcgaatttta ttagtgatga tgcaattcct ttaccagaca
    64141 tactcggacg taccattaaa aggtgaatta ccattacctc atcttcagat aatttttctt
    64201 tccaaggaat agttgcatat tctattggtt gaactttatc aataatgata cttccaacaa
    64261 ttgttccgtt ttcttcatat acaaacaaag caccagcata aatagcccgt tctgcatcat
    64321 ctttcgttgg ataaacccct ttcttaaata cagtaaaggc tgtatgattc agttcatact
    64381 ggaaatgctc attgtaagta tcttcaatcg aattaacatc ttttagtatt gcttttctaa
    64441 tcataaaatc tctccacaaa ttttgatttc acactttctt tccccttata tgtctatatc
    64501 tgaaatattc agtcacaaac tttatcaggg ataggcatct gtgccgcaag gggatcttcc
    64561 ccttgtacga aatctctgat ttcgctgtat ttcccggttt gagatttctt gaaatcggga
    64621 acagcaccgc aggtcgcacg atacaggcag atggaattgc ctgtatagaa gtgcgctctt
    64681 agcgttgcta agagatttat gccgtatccg gcgtgttatc ttttttcaat ttcttttttc
    64741 gacttctgaa gcattcttcc agttttaata atttgacatg tctgctcttg ttactcatca
    64801 tctgtaaatt ccagcatctc atcaacggtg cagtccagct tcttgcagat cttctcaatc
    64861 gtctccattg atacatactg gttttttccc atcctggcaa gaatatttcc actgatcccg
    64921 gtgaggtact gcatctctat ccgcttcata tttttatcta tgagcatttt ccataatctg
    64981 ttataactta cgcccataag caacctccat atttttcatc tgaacatatt ttgattatat
    65041 cacgatttcg ttaggttgaa tacctgtaat ttttgatgtc ttctgtaaaa tggaaaagag
    65101 gatgccccga caaagggcaa cctctccaac catttacttc ggtttagtcg aatggaacct
    65161 caccttccgg tgcttccata aatccgtcct tatctgtttt cggtgtttcc gatgaatcag
    65221 ctccaacacc attatccttg ttctgggcaa attcatgttc ttccacgatc acatctgtag
    65281 tataaattgt tttcccgtcc ttgtccttat aattgccagt gctgatacga ccgccaatca
    65341 caatcctcat tcccttgtga agatatttct gtgcaaactc tgcactcttt ccgaatgtga
    65401 cacaggaaat aaaatccgct tcctgttcac cgtcacgttt atatcttctg tttaccgcca
    65461 gtgtatatct tgctactgcc atatcattat cttttccggc atatttgatc tccggattct
    65521 ttgtcagtct tcccattaaa accactttat tcataatcgt tactgtcctt tccgcagcta
    65581 cgctgccatt gcatattctt catcatctgt ttcatcgtcc ggcataaaca ttgcacatgc
    65641 ttccggtgga ttggtatcat ccggtgaaaa tattttccgg atcatttcat acatgatcgg
    65701 ataccgatat gcgatttctt ctcttgcaag gaatctgcat accctgtaaa gattcttttt
    65761 catttcttca tctgttgtaa gcatggcaac cagatggatt ctctcccttg tataaatcag
    65821 attcgggcat ccaaaccagt taatcaactg catttctttc attttcagtt ctgtcataaa
    65881 ctgctccttt ccgggcattt tgccctgtaa acattgtcat cagtttcatt cgtccggcgg
    65941 tctggcgtgc gtttccctgt cacctgcagc cgtctcagac tgcctctgcc tgcatcagca
    66001 ggtcactgac cataaggctt cggtcagccg cccatcatcg ggagtgtcat tataatttcg
    66061 ctcgctctca gaaaaagaaa ggtcatggca taactcctgt taggcttcta gggtgcactt
    66121 atcggttgtc aaggttcatc gaaggaacat tccaaaacgt cctctgatta ctagagtcaa
    66181 atgacttcgc aagatgcaaa agattcgtga tatttttttg catttttatc attttttctt
    66241 ttacttttgt tagtcccttc acatactctg tactgagaca ggcaaaagcg gacatctttg
    66301 gggtaaaatt tttaaaataa ttgttcctct acctataaag cctgggaagc ggtatttacc
    66361 aacccccttt tgcaagtttt tttcgcataa atgaaatatt ccctcactaa tagcaagttt
    66421 cagagggcat ttggacggta cttttgcaga aaaaattatt tttccactaa tcacacgtgg
    66481 gaatttgaag aatgtcagat aattttgaaa aaatccaaaa aagaagcacc gaccgttata
    66541 gtcagtgctt gtaagatatt atattcgttt ttctcaggaa agatcatccc agtttttata
    66601 tgttccattg tacagacctg taagttgttt tattgtaaga ttttccagtg gattttcttc
    66661 atttatcaca atagctatag catcttttcc aatgactttt gtttccagaa gctctgcttc
    66721 ataatccttt aattctctgg atgacattgc gaaatcacac tctccactta tcaccgccgt
    66781 aatccctctt gaagaatctg ttgcttttac ttctatttca gcatttggat tttgtttctg
    66841 atagtcatct gccagtgctt ttaccattgg ttccattgaa gtggatcctt caatcagaat
    66901 ctttccttct gacttgtcag aaaggaatgt tgttgtactg tcagcctgaa tacaatattg
    66961 tttcacaata tcctgtcctt tactctttac ataagtcagg aaatcctgtt ccacatctgt
    67021 cagttctcca ttataagcca gataataatc cctgcaaaga ggataactgt tattgtctat
    67081 tgttttctca gaaggaagaa ctccgttgat ttgaagtatt tttccattcg tatctgttgc
    67141 ggaactatat gcgacataac caactgcgtt tttatcttct tccacctttt tcagaacttt
    67201 ctctgtagaa tcaacaacta ttcctgtatc actctcaggc agtttcaaaa gattctcaaa
    67261 ttctgctctg gttcctgacc cttcttctcg tgatacagct gtgacagctc ccatagcaga
    67321 cagatccggc aatggctctt cctcggttcc acatgctgtt attcccgttg ccagcattat
    67381 cattatcagc agaagtgcag attttcggat gttttttttc tttaccattt tatactgcac
    67441 ctctttccat cattgatttt cccgtctctt catcaaggtt tataaaagcc acattatcac
    67501 aggcttcttc tgcaatgctg acacaattat ctgccatacg gtcaaaatta tacatgatcc
    67561 cagaaaaata tcttgtcata gacgcatcac aaacctcatt ttttacacgg tttaaatgtg
    67621 ccttactgta ttttttctct gctttatgca tttttttctt atttttaaaa actgtttcag
    67681 ccactacagc atcaccctta cggattgcct ccattgcttt ctttaaaagc tgctggataa
    67741 tatcaatgca gtcttccatt tctgaggagg cttcactggt cagtcttttt ccattgttat
    67801 ttactttttc acatatctgg tcaatatctt cacatctgtc tgcaatcctc tgaatactat
    67861 tgtttacata cagtaaccct gccgtctgct cagactgttg ttctgtaagg tttccgcttg
    67921 cgaataatct ggtaatatat tctgtcacac tgtcctgtaa ctgatgtaca atctccagat
    67981 tttctataaa cgaaccatga gtttttgagt ctccatctgc tacaatcgcc gtttttaaat
    68041 cacttaacag agattctgcc agttctccaa gtcggttcag ttccttagat accagaacca
    68101 tagcagccgc cggctgttca atcactttca tatccagata tttgggttca aaagctgctt
    68161 tctctgtttt gtcatttcca cggatgattg ttgtaacaat ttttaccatc agcggaatca
    68221 gtggcagcca gaccagtgta cagacgatat taaacgttgt atgtgcattt gcaatctgtc
    68281 ttgaaatcac atcaatctca tttccctttg gtgaaataaa ctggacaaat tttgcaaacc
    68341 acggaaccag gaaaataaaa acgcagcttc cactgatatt aaaaatacta tgcgcaattg
    68401 ctgtacgttt tgcattttta gattgtccaa ttgatgcaag aagtgctgtg atcgttgtac
    68461 ctatattatc tccaagaaga atcggaatcg caccggtaag ccctattaca ctgcttactc
    68521 cgtccggtcc tgcctgtgat gcaaaattct gcagaactgc aatcgtagct gaactactct
    68581 gcacaaccaa tgtcatgacc gctcccagta caacaccaag aaccggaatg gatgaaactt
    68641 ttcccatcag atcaacaaag accggacttc ccgccaaagg tttcatgact tcacccatga
    68701 tttcaattcc ttcaaataaa agtccgaatg aaaaaattac cataccgata tttttaacct
    68761 tttctttttt gctaacaaag ttcagaataa atccaacaaa gataattgga taaatataat
    68821 tgctgatttt aaatgccatc agctgtgccg tcatagtggt tcctatgttg gcaccaaaga
    68881 taacagaaat tgcctgcgga agactcataa gtcccgcact gacaaaaccg ataaccataa
    68941 ctgtcgtcgc tgaactgctc tgcaatacag ctgttacaag tgctccggca agtgctccca
    69001 taattggatt tcttgtaaga aatccaagga tttttttcat tcgttctcct gcggctttct
    69061 gaagtgcatc actcatgctg ttcattccat aaaggaacaa ggcaaggcca ccgatcagac
    69121 caaaaaccac tttcacattc tcgttcattt ctctttcctc tcctgttatt atataagatc
    69181 tacattttcc tatacaatta tagaagacag aaatcaaatc tatgtgagtg cttgtgtaaa
    69241 ataggtgtaa aaatcaatgg aaatctttat aatgttataa ttgacttatt catttttcac
    69301 ctgctgacat atagttttca ttacgattta ttattcagtc tacgtaatac caacatcgcc
    69361 tcattcctta catttttgaa cgttctgtcc ggtcttccaa gaaaatatcc ttgtccgtaa
    69421 tgaacaccta atctacacag acactccaac tctgcttctg tttctatccc ttctgcaatc
    69481 agaattgctc ctgtctcatt acagtaatgc aacagaaatt ccatcataat ttcctttttc
    69541 ttatgttttt gaatatctct gaccagttct atatcaactt ttaagtagtt tggcgatgta
    69601 tttactacac ggttcagacc agaatatcca gctccaacat catccaaagc tatttcatat
    69661 ccctgatctg cataatgctt cataatctgt tgcagaatct gatagttttc tatatccgac
    69721 cgttccgtta tttcaaatac aatgtcacag gacggtactc cggctttcgc agccttacgg
    69781 tttgtaaatc cctgtatgaa cgatttatcc tgaatgatat taccatctac attaagaaat
    69841 aattttgcat gttctggttt attcgccgct gccttcaatg ctttatttct gcacaatttt
    69901 tccaatttcc aaacacagcc tacctgttca gcaatcacaa acaaatctga aatcataaga
    69961 gtcgtatcat gcctgtcgat acgactaaga gcttcatatc ctgccacatc tccagtctgt
    70021 aaatttacaa tcggctgaaa taccggatat atagcctttc tttctaatat tttataaaat
    70081 tcatctgcat tttttacttt cattatttct tctctgcctc tgtgaataca ttttctgtaa
    70141 ccctatcaac ccactatatg atatcatcac tttttcatcc ttcaatcaga tttaagtaaa
    70201 atcatagtaa attttcacca ttgttcagcc agaccaattc ttatataaca caaagcaaat
    70261 acgtactacc acctgttttt tattatgaaa actatattaa atgaggctct tactgtctat
    70321 acaataaaag cctcgttata tatagtctat gttctgatta ttcagatctt actgtctttt
    70381 tcacacacca gtctttcata atccagaaat ccccggatat acatttcttt tgccacaatt
    70441 ccctccattg catttttcgc tgaaataatc ctgtccagtg tctgccacat ctcctgtccg
    70501 ccatctctga ggaccggttc cagtatttct atgtaatgat gaatttctgc ttccagagtg
    70561 aacatctccg ggtcagattc aagaaatctt tccagctccc gctggtattc ttcataataa
    70621 ctttcaatca gttctcttct catatctaga atccccctta tacataaatc agttcctgct
    70681 tgatgatctc agcagcccgg ttgcggatat tgttacagtg gcatagccat tccataggat
    70741 tctgtctctt cagttcctcg gaaactccct ccgcacttcg catctgagtt tcgatcaatt
    70801 caaagcgttc ctgtgcctgt tcattcagat ctgccaaata actgtccagc ctggcggtca
    70861 gaagaagata tgtataagtt ccagatttat gttctttcag atattccttc cgaagcattc
    70921 cccataagcc aatcggtctg gtttcctccg ggtagtaaag attcggtaaa taatagtctc
    70981 ccacttttgt gtattcgatt ttcatggtgt ctcctttcgc tttgcatgaa attgtcatgt
    71041 tatctgcgta ggatgtgcat tttctcttta aaatcttttc acatagaaga cattaagtgc
    71101 ttgacaccct gcgattttgc ccccggattg tcctgcccgt atccctccaa cagattgatg
    71161 ccgccccata cgcacagagc gccgcccagt ccgatcacga tagtctgcaa agtagtaact
    71221 gcggaattaa aaaatgccat aaatttacct ctttctgccg gtttccggct cataatgatt
    71281 ttttgatttt gttgttggaa tgaaaaaagc cgctgtttat tcagcggcgg cgtcctcgtc
    71341 cgacaggtcg atttcataca ggtcgaatac ttcatcctgc gttaccactt gctgccgttt
    71401 ccggcgcgtg atagtcaaaa atctgtccac gtcaaaggta ttcttcttgt cggcgtcggc
    71461 aaggtatttg taccggggat gcttggtaat atcgaatttc tccgataaaa acgggcgcac
    71521 accccgtagc tgcaagatac acttgccgcc gtccatcacg gcaatttcgt cttgggacat
    71581 caactccttt cctaattttt gatagttcaa tccgtgggac gtttggcttc cccggttctc
    71641 gctggtgttg aaactgtcaa tcgtttcttt ccccagcgca tcggaaatct ccttggcatt
    71701 tttgccgcgc ccactaagga aaagcacact atcgcagtta tccgaaatga tctcggcggc
    71761 gtccttgtaa atcgctttta gctgcgactg gctctgcaaa ataatagagg ccgatatttc
    71821 acggcttcga atggtggcaa tgagtttatc gaaattgggt atttggccaa tgttggcaaa
    71881 ttcatccaaa atcaggcgta catggacagg cagccgcccg ccgtacacat catcagcccg
    71941 gtcacaaagc aggttgatta gctggctttg gagcatagcc agaatgaaat taaaggtcgt
    72001 gtccgtatcg ctcataatga gatacagcac cgtcttttcg tccccgatgg tgtccagctc
    72061 catttcgtcg tcctccatca gctcgcgtac ctcccggatg tcaaagggcg caagtctggc
    72121 accgcatgaa atcaggatgg aagaacgggt cttgcctgcg gacagcagga atttcttgta
    72181 ctggcgcacc gcaaaatggt cggggtcttt ctgctccaaa cgctcaaaca tgagatcgac
    72241 ggggctttga aactccgggt cgtcctcgcg ggcttcactg gcgtttatca tttccagcag
    72301 cgttgagaag ttcatttcct ccgctggggc ctcgtaccag atatagccga tcagggccga
    72361 ataaaacaac cgttccgact tcacccaaaa atcctccgct gacttttccc cctcgccctt
    72421 ggtgttggaa atcagggtat ttaccagctt tagcacgtcc ttttccgaat ggatgtagac
    72481 aaaggggttg tacttcatgg acttggaaaa gttaatggta ttcaggattt taatgcggta
    72541 gcccgcctgc tggaacatcc tgccggtttc cagaaccaaa gtgcctttcg gatctgtcac
    72601 cacaaacgaa gtcggatagt ccgtggtcgg aaagcattgc atcaggtatg aggtaggcac
    72661 cctgcgcctt gctgactttc atcagtaggt ttcagggagc cgcctcccaa accggacgta
    72721 cacctctcag cgtatccggc tttccatgaa tcaccttgat tctttggttt cgtgtaagag
    72781 ttcaaagcag tcagggcaga gagccagaga ttttcttctc attttcagca ttttctgctc
    72841 aaatatatcc ctgcctttca gcgatttcag cgttcttacg tggtgcatac ataaatagtc
    72901 tgcatgttcc ccgcaaatct cgcatactcc ggcttttatg cggtttacta tcgtgtgcct
    72961 gctgtcatat ttcctataac tcggcataac gtctgccaca ttgtcatagc cgtcgctgtg
    73021 ctttctgaat ccatcatggt aaaattcgca gtatttacgt cccttagttg tatcgtatgg
    73081 gattctcagt actccgttta ccatgtgccg cttcttaata acactgacat tcgttctgtg
    73141 ctttcctgcc agagttttta acatgctgta ccgcaccatg tagtaatact tgtgcaatgc
    73201 gcctacgttt tctgctatcc gatagaagtt atataaccct cgaatttcgg aattgaaagt
    73261 agacacaatc tctgcgtcgc tgcggttcat caggtctttc cttggcatag gccgccacat
    73321 ttctttgcct gtgtcattgt tgcgtttcac ctgtatcgct ccccgttcca tcgctttttt
    73381 aatccatttt tctttcggca tatacaggaa tacttttcca taccacaccc gtttcatgtc
    73441 gccgttttta caacgcttca tgttcttgga atggattact ttgaaatcgt accccagata
    73501 gcgtaccggc ttactggaat gggtgacttt cgtcttttcc tcggacattt ccaaatgcag
    73561 tttttcttgg agaaagattt ttacatcttc cttgattttt tctgcgtcct ttttggagcc
    73621 gataacgccg attacaaaat catcggcgta acggttgtac tggattttct tgaagccttc
    73681 ctcaaacgga ttgtaatagt gctgattcat cttcttcctt ctggaatcct taaattcttt
    73741 taccagttca ggagttgatt tttctgcgcc cattaacgct ttacgtgcct ttctgtatct
    73801 ccgggacgcc cgctcgtatt ctctggtcgt ccttctgcgt tctggctcgc aatcatactt
    73861 ttctttgtat tcctgcatat agttgtccag ttcgctgagg tagatgtttg cacatatcgg
    73921 actgataccg ctgccctgcg gaacaccgga gtaggtgcag ttgtactgcc attgttccat
    73981 atatccggct ttcaggaact tccagattag accgataaaa gcctcgtctg aaatcctttt
    74041 ccgcagtaat tcaaccaaca catgatggtc gaagttatca aagcacgcct taatgtctcc
    74101 ctcgacaatc catgtaacac ctgtgaagtt tttcttcact tgcgtaagtg ccgtgtgaca
    74161 gcttcttttt ggtctgaaac cgtgtgaatt gttgctgaat gtcggctcat agattgcttc
    74221 aagaatcatt ctgaccactt cctgcactaa cttatcatca gtagaagtga ttcccaacgg
    74281 gcgaagtttc ccgttctttt taggaatgta ctttcttttt gcaggctttg gctgatatgt
    74341 cctgttgcgt atggattcaa taatccggtt aattctcggc aggctcatgt tgtccagcgt
    74401 ctgcccgtct accccctgcg tcatgctccc ctgcgatttc gcaatgttcg catatgcaag
    74461 gagatagaac tctggattgt ataggttgcg atataatctt tcatatttgt agttacttac
    74521 acttgccttt tcttcaaggc ttttcaagac atgaatcgga tttctcatag tgtctcacac
    74581 accatccttt ctgttttgaa aatgattcac tgttcccctt tgccatgtag acggctttcc
    74641 cgtccgcaga ctactatggg aactctgttg ccatggtgaa tattcagaac cccgcatttc
    74701 agcgttttca tagcctttcg gcatttcact ttaggcaatc cccgtttagt acggtataaa
    74761 caagcgttcc acgttgtcgg tatgtgacgt tcgccttttt ccactttccc gtggctgaac
    74821 ctgctccgag tatcagcagc cgcatagcca aacaatgcga ctgtcaggaa cactgacgag
    74881 tacaacctct ctacgtcagg ggtacgctct tggtcccgat ttcaggctgt cattcaagca
    74941 gtctagcttc catcctcata cgtggatttt cattccgtca cgccggcgct tctgaggctt
    75001 cagcgccccg tgtcctccgt catgctatgg tcgccctccg gtttcccttt tggggtaaga
    75061 cggggaatga taggttgcgg cacaatgccg cttgatactt ttacctacta cttgctcaag
    75121 gtaggattta taccgccctt acgggcgcac tgggtttcac gaaaaatctc gttttccctg
    75181 aaccgctgcc accgatcacc agcacgtttt tattccgcgc tgtcttgggg tctttgggcc
    75241 tgccggtcat ggtaagccgt tccgtttggg tgagcaggat attattttca aacttggggt
    75301 cgatgtaagg cgctatgtct ttggccgtcc cccagcgggc gctgccatac tccatgcctt
    75361 tgcggtattt cttcacgttc ttgctcttgc agtacaccac cagccgcacg atcacggcac
    75421 cggcgatacc gatacacagg tcaaacaggt gcaggcttgg cagcgggttg gcaaaagccg
    75481 ccgcaaaacc atccgcaaaa tgcaggagct tccccgacag gtcagcacca gcggccagcc
    75541 ggtacgcctc cccgcatttc gcaaacaggt acacaaaaat cagatacggg aggttcagga
    75601 tcagcaattt ctttacgtca ggcttcacag ttccagcccc ctttccttga ttttctcctt
    75661 ggcccgctgc ttggtctggg ccgccgcctt ttgcttcaca gcggacaaaa ccttgcggat
    75721 agagggccgc tgctccttgg aaagcgtctt ggcggtaaac tcccgaaacg ccagatcaag
    75781 gttatctgca tccttggcct tgaaaatcac aacgtagtgg ggcggctggg tggtcttatc
    75841 ttttcgtaag gtaaaatcca cgccgtattt cttggcgcat ggcttgaacg ctccaatgtt
    75901 ggcctcggtg atctcaatgt tggagagatc agccccatgc tttttaagct gctttaggct
    75961 ctgctggccg tggtgcagcg tgtttttgcc cttggcccgg tgttgcaggt actttttcag
    76021 cgccgcttgc agcacttgag ccgtgagctt gcccgttttc atggacaggg caatggtctt
    76081 ttgagtaact tcttcctgca atcgcatccc tccttgtcag aaagtcaaaa gcggcgtcta
    76141 aggcggaacc accagacgcc gcaggtagat ataggtcaaa tccgcacccg caggccggaa
    76201 aggtcgtcgg cccggatgcc gacaagatac cagttttcgg ccatttcaat gcgggtgggc
    76261 ctccatttgc cgccgatcat cacgtcgaaa cactccccgc aatgcaggcc gccgtaatag
    76321 tcggccagat caaagcggat gtcgtaacgg tctgttcgct cgtcaaaaat caatgcgccc
    76381 tgcttcatag ggtcgtcctc cttttctcgt tacaggcttt cgccctcgcc gcaggaatag
    76441 tccggcaggc cggatttagg ctttccccaa tcgggctgtg ccatgtcgtg cgcgacaagg
    76501 gccgtgtagt agttggaaat ggtgctgggg gcgttgaaca gcatagcccg caggtattgt
    76561 ttgatattgc ggactttcgt tgtgttctcc cgcagacagt caaggacaaa gcggatatgt
    76621 tcgccggtca gcttcatgta cttggatttc accagctcgg cagggtagtc gtccccggcg
    76681 acacggattg tctttcgggc ggtgcagacg gtttccagca tcaggtctac gatttcgtca
    76741 agctgttcac ggtcaatgct cctgtcctga atgagatagg catactcgat gttctccaaa
    76801 atgatttccc gataaatccg tacagcctca tcctttcctg tccttttccg ttccggcggc
    76861 gtagccgctg cctcgccctc cataggggca ggggtcgggg atgggatagg aatggaatgg
    76921 gtactttgtc catctgtaat tattttttct ttttttgata agtcagttct ttgtacttct
    76981 ttatttaatt gcgttggatt ttccgacgcc ggattatccg atgtcggatt ttccaacacc
    77041 gggttatccg atgttggatt ttccaatacc gggttttcca ataccggctg ttcgtagatg
    77101 gtatagacca tatcggccat tttgcctttt gcgtcacggc cctgctcacg ccggatatat
    77161 ccagcttttt caagctccca taccgctgtc ctgatcgcgt cgatgctttc ccggttgata
    77221 taggacagtc cttttagggt atagtcccaa tcctccggca aggacaacat ctgcgacagc
    77281 aggcccttgg cctttaaggt caggttctta ttccgcaggt ggtgatttga catcaccgta
    77341 tagcctttgt tcttttccac acggaaaact gccatagcgt tgttcgctcc tattacaata
    77401 aatttggaca ggaaagccgg acagccagtc cggctttccc tccatctcgt gcctaagcta
    77461 caatgagagg agcaactggc tgaccaatca ctccttgggc ttgtatgtcc gtgtgaaaga
    77521 ctgttagcca tcctctcgtt gcagcgttcg atttaagcag gttgaaccac cggatgtcgg
    77581 agagttcgtg tcacacaata gattgaatcg gcagcgagaa aagatggatt ccggttgcag
    77641 attctttgta ttggaggaat gtgaatgaac tgcgttggca tcgatgtttc caagggtaag
    77701 agcatgattg cagtcatgcg gcccttcgga gaggtagtgg tttcaccctt tgaagtacgt
    77761 cacaccgcca atgaactgag cgagctggca ggactgctca aaagcctgga cggcgagacc
    77821 cgtgtggtga tggaatccac gggtaattac catgctccgg tggcctggct gctccacgac
    77881 gcgggccttt atgtctcggt agtcaatgca atgctggtgc atgactacgg aaacaacagt
    77941 ttaagacggg ccaaaaccga caaaaaggat gccgtaaagc tggccaacta cggtcttgac
    78001 cactggctca cattgccgag atatatcccg gaggaggata cccgactcat gctgaaaatc
    78061 tgctaccggc agtaccaaca gtattccaaa gttcagacca tgctgaagaa taacctgatc
    78121 tccctgctgg acaccacctt cccagacgca aatcgcttgt ttaccagtcc gccccgcgcc
    78181 gatggcagcg agaagtgggt ggactttgtc gctacttttt ggcattgcga gtgcgtttgt
    78241 ggccggtctg agaaggcctt tactacccaa taccagaagt ggtgcagaaa gcacggctac
    78301 aatttcagcg aggataaggc gctggatatt tatgcctctg cttgcagaca cttcggtgtc
    78361 ataccgaaaa cggatacggc aaaacttttg gtagaacagg ccatttctca actccagaca
    78421 acttcttccg cgttagctgc tctcaaacag gagatgcaat ccctggcagc ttctctgccg
    78481 gagtatcctg tagtgatggg gatgtttggt gttggcccaa ccctcggccc tcaactcctg
    78541 gctgaaattg gcgatgtacg ccgttttcat tccaaaaaag cgttggttgc ctttgcgggt
    78601 attgacgccc cgccctacca atccggtcaa atagatgtcc gtagccgtag catttccaag
    78661 cgaggatctg cctcactgcg caggacgctc tttctggtga tgagcgtcat cctgcaatgt
    78721 gctccaatag acgagccggt gtaccagttc atggacaaga aacgctctga aggcaagcca
    78781 tatcgtgtct acatgatggc ctctgccaac aagttcctgc gcatctacta cgcttctgtg
    78841 aaagcctact tggaatccct ggagcacgac tgatttttac actctatacc atctggctgg
    78901 ccgccgtttt aattttgaga tgctcagcgg cttgattttg tattgcattt tttcgcctcc
    78961 ctaaaaatct gaaatttcta cttgactttt attagcaggt ctttcactgt gaatttgacc
    79021 gctgcatagt cggtcagctt gcccggctga tagggacact ctgcaaactg gcagaattga
    79081 tatttccaat ccgggcggta aaatcggcag gtgccgcaat cctccggcgc gtccgccccg
    79141 ccgccgtcgt agtgatcgaa accgggcttt tcctgcatca gccgttcaaa cgcccggtca
    79201 gggccggaag taaatagcat ggcgtcgcct cctttctcta tttgggcata aaaaagcggc
    79261 gtcctgttct cccatgacgg gaaagaggaa cgccgcgttt acggtgtcgt attcaattta
    79321 tgatgtgccg tatttggggt ggcgggtatt agcagtatgg ttcagcatat atgcggctca
    79381 atccgaaagt agtaaattgg tagtaaattg agttctcata tcctgcgaaa tgcccgtatt
    79441 tccggggctt tcagcgataa cctaaaattt tgaggaaaaa tcttcgtggt tgttttcaac
    79501 ggctattacg cgacgcgtcg agattttttt tcctgaattt tcataatatg taataatatc
    79561 tccgactaaa atctcatttg tatttttcgg tcgtacatat atcagatttc cttgtgcata
    79621 atatggactc atttctttgt cctcaataat ataaggagtc aaaccaaaaa ttcggggaac
    79681 caagacgacg agaagaataa gaattaaaaa tataatcaac gttatagaac ttataaaagt
    79741 tatttttttg taatcaggta tcgttttcaa cttctttctt tattcaatcc ttcttaatca
    79801 tataataacg ataaattttt ttca
//
LOCUS       DAHHPA010000002        68507 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.002, whole genome shotgun sequence.
ACCESSION   DAHHPA010000002 DAHHPA010000000
VERSION     DAHHPA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.002"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            186..1067
                     /locus_tag="NBH55_000091"
     CDS             186..1067
                     /locus_tag="NBH55_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1508109.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1080..2690
                     /locus_tag="NBH55_000092"
     CDS             1080..2690
                     /locus_tag="NBH55_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCD1508110.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2767..3426)
                     /locus_tag="NBH55_000093"
     CDS             complement(2767..3426)
                     /locus_tag="NBH55_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCD1508111.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3709..4374)
                     /locus_tag="NBH55_000094"
     CDS             complement(3709..4374)
                     /locus_tag="NBH55_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCD1508112.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4521..5126)
                     /gene="dhaL"
                     /locus_tag="NBH55_000095"
     CDS             complement(4521..5126)
                     /gene="dhaL"
                     /locus_tag="NBH55_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCD1508113.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5154..6164)
                     /gene="dhaK"
                     /locus_tag="NBH55_000096"
     CDS             complement(5154..6164)
                     /gene="dhaK"
                     /locus_tag="NBH55_000096"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCD1508114.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6139..6585)
                     /gene="dhaM"
                     /locus_tag="NBH55_000097"
     CDS             complement(6139..6585)
                     /gene="dhaM"
                     /locus_tag="NBH55_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCD1508115.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6688..7008
                     /locus_tag="NBH55_000098"
     CDS             6688..7008
                     /locus_tag="NBH55_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCD1508116.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7120..7989)
                     /locus_tag="NBH55_000099"
     CDS             complement(7120..7989)
                     /locus_tag="NBH55_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1508117.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8085..9389)
                     /locus_tag="NBH55_000100"
     CDS             complement(8085..9389)
                     /locus_tag="NBH55_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1508118.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9386..9895)
                     /locus_tag="NBH55_000101"
     CDS             complement(9386..9895)
                     /locus_tag="NBH55_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508119.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10070..12205)
                     /locus_tag="NBH55_000102"
     CDS             complement(10070..12205)
                     /locus_tag="NBH55_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1508120.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12288..13943)
                     /locus_tag="NBH55_000103"
     CDS             complement(12288..13943)
                     /locus_tag="NBH55_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD1508121.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13945..14259)
                     /locus_tag="NBH55_000104"
     CDS             complement(13945..14259)
                     /locus_tag="NBH55_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD1508122.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14429..14689
                     /locus_tag="NBH55_000105"
     CDS             14429..14689
                     /locus_tag="NBH55_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCD1508123.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14703..15017
                     /locus_tag="NBH55_000106"
     CDS             14703..15017
                     /locus_tag="NBH55_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCD1508124.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15747..16301)
                     /locus_tag="NBH55_000107"
     CDS             complement(15747..16301)
                     /locus_tag="NBH55_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCD1508125.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16427..18355)
                     /locus_tag="NBH55_000108"
     CDS             complement(16427..18355)
                     /locus_tag="NBH55_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCD1508126.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18661..19377)
                     /locus_tag="NBH55_000109"
     CDS             complement(18661..19377)
                     /locus_tag="NBH55_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCD1508127.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19378..21201)
                     /gene="glpO"
                     /locus_tag="NBH55_000110"
     CDS             complement(19378..21201)
                     /gene="glpO"
                     /locus_tag="NBH55_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCD1508128.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21215..22711)
                     /gene="glpK"
                     /locus_tag="NBH55_000111"
     CDS             complement(21215..22711)
                     /gene="glpK"
                     /locus_tag="NBH55_000111"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCD1508129.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMKEERREKLYNGWKKAVKATQAFE"
     gene            complement(22847..24307)
                     /locus_tag="NBH55_000112"
     CDS             complement(22847..24307)
                     /locus_tag="NBH55_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508130.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24297..25592)
                     /locus_tag="NBH55_000113"
     CDS             complement(24297..25592)
                     /locus_tag="NBH55_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD1508131.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25924..26601)
                     /locus_tag="NBH55_000114"
     CDS             complement(25924..26601)
                     /locus_tag="NBH55_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCD1508132.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26631..27722)
                     /locus_tag="NBH55_000115"
     CDS             complement(26631..27722)
                     /locus_tag="NBH55_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCD1508133.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27823..28257)
                     /locus_tag="NBH55_000116"
     CDS             complement(27823..28257)
                     /locus_tag="NBH55_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1508134.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28841..30298)
                     /gene="ascB"
                     /locus_tag="NBH55_000117"
     CDS             complement(28841..30298)
                     /gene="ascB"
                     /locus_tag="NBH55_000117"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCD1508135.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30295..32190)
                     /locus_tag="NBH55_000118"
     CDS             complement(30295..32190)
                     /locus_tag="NBH55_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1508136.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32248..34023)
                     /locus_tag="NBH55_000119"
     CDS             complement(32248..34023)
                     /locus_tag="NBH55_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCD1508137.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34073..35569)
                     /locus_tag="NBH55_000120"
     CDS             complement(34073..35569)
                     /locus_tag="NBH55_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD1508138.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35763..37385)
                     /locus_tag="NBH55_000121"
     CDS             complement(35763..37385)
                     /locus_tag="NBH55_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCD1508139.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37834..39147)
                     /locus_tag="NBH55_000122"
     CDS             complement(37834..39147)
                     /locus_tag="NBH55_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCD1508140.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39392..39616)
                     /locus_tag="NBH55_000123"
     CDS             complement(39392..39616)
                     /locus_tag="NBH55_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508141.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39636..40058)
                     /locus_tag="NBH55_000124"
     CDS             complement(39636..40058)
                     /locus_tag="NBH55_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCD1508142.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40201..40911)
                     /locus_tag="NBH55_000125"
     CDS             complement(40201..40911)
                     /locus_tag="NBH55_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508143.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40929..41426)
                     /locus_tag="NBH55_000126"
     CDS             complement(40929..41426)
                     /locus_tag="NBH55_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1508144.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41463..41915)
                     /locus_tag="NBH55_000127"
     CDS             complement(41463..41915)
                     /locus_tag="NBH55_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1508145.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(42050..42655)
                     /locus_tag="NBH55_000128"
     CDS             complement(42050..42655)
                     /locus_tag="NBH55_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCD1508146.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(43105..43725)
                     /locus_tag="NBH55_000129"
     CDS             complement(43105..43725)
                     /locus_tag="NBH55_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1508147.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43727..44596)
                     /locus_tag="NBH55_000130"
     CDS             complement(43727..44596)
                     /locus_tag="NBH55_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCD1508148.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44619..45026)
                     /locus_tag="NBH55_000131"
     CDS             complement(44619..45026)
                     /locus_tag="NBH55_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD1508149.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(45045..46334)
                     /locus_tag="NBH55_000132"
     CDS             complement(45045..46334)
                     /locus_tag="NBH55_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1508150.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46361..46663)
                     /locus_tag="NBH55_000133"
     CDS             complement(46361..46663)
                     /locus_tag="NBH55_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1508151.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46688..48781)
                     /locus_tag="NBH55_000134"
     CDS             complement(46688..48781)
                     /locus_tag="NBH55_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1508152.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49270..50034)
                     /locus_tag="NBH55_000135"
     CDS             complement(49270..50034)
                     /locus_tag="NBH55_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCD1508153.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50145..51014)
                     /gene="mdcB"
                     /locus_tag="NBH55_000136"
     CDS             complement(50145..51014)
                     /gene="mdcB"
                     /locus_tag="NBH55_000136"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCD1508154.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(51039..52115)
                     /locus_tag="NBH55_000137"
     CDS             complement(51039..52115)
                     /locus_tag="NBH55_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCD1508155.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(52139..52732)
                     /locus_tag="NBH55_000138"
     CDS             complement(52139..52732)
                     /locus_tag="NBH55_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCD1508156.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52736..54412)
                     /gene="mdcD"
                     /locus_tag="NBH55_000139"
     CDS             complement(52736..54412)
                     /gene="mdcD"
                     /locus_tag="NBH55_000139"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCD1508157.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVSLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54402..54701)
                     /locus_tag="NBH55_000140"
     CDS             complement(54402..54701)
                     /locus_tag="NBH55_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCD1508158.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54803..56449)
                     /gene="mdcA"
                     /locus_tag="NBH55_000141"
     CDS             complement(54803..56449)
                     /gene="mdcA"
                     /locus_tag="NBH55_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCD1508159.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56456..57367)
                     /locus_tag="NBH55_000142"
     CDS             complement(56456..57367)
                     /locus_tag="NBH55_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCD1508160.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57487..58344
                     /locus_tag="NBH55_000143"
     CDS             57487..58344
                     /locus_tag="NBH55_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1508161.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58531..58893)
                     /locus_tag="NBH55_000144"
     CDS             complement(58531..58893)
                     /locus_tag="NBH55_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCD1508162.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            59239..60678
                     /locus_tag="NBH55_000145"
     CDS             59239..60678
                     /locus_tag="NBH55_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508163.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60685..63237)
                     /locus_tag="NBH55_000146"
     CDS             complement(60685..63237)
                     /locus_tag="NBH55_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCD1508164.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPKGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYELGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63351..64682)
                     /locus_tag="NBH55_000147"
     CDS             complement(63351..64682)
                     /locus_tag="NBH55_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1508165.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64648..66126)
                     /locus_tag="NBH55_000148"
     CDS             complement(64648..66126)
                     /locus_tag="NBH55_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1508166.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66431..68266)
                     /gene="tdc"
                     /locus_tag="NBH55_000149"
     CDS             complement(66431..68266)
                     /gene="tdc"
                     /locus_tag="NBH55_000149"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCD1508167.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19509 a        14592 c        11715 g        22691 t
ORIGIN      
        1 taactatcta ttgagaaagt atcaagctag caattccttg tttatgaatt cattgacaga
       61 aatttataaa aagaatatta tgtactaaaa taataaaaaa agttttataa aaaggtataa
      121 aaccttttta ttaaactatt taatcttgtt tagtatatat ttttagaaaa aggagtgggg
      181 tttttatggc agaaatcgtc aaagtcacac atttgaaaaa gagctttggt cgtttcgaag
      241 ctttaaaaga tgtctctttc accgtacatt caggtgaagt cgttggattt atcggaccaa
      301 atggttctgg aaaatccacg acgattcgga cacttttagg gattataaaa aaagacggcg
      361 gaacggcaac gatttttggg caagatgtat gggaagatag tttatctatc catgaacgtc
      421 tttcttatgt tccaggcgat atctctttgt ggggaaattt gactggagga gcgatcatcg
      481 atctcttcat gaagcttcat ggaaaaggga gtgctcaaaa aaaagatgaa ctgattcaac
      541 gtttctctct tgatcctaag aaaaaagcaa aaaactattc aaaaggaaat cgccaaaaag
      601 tgggcttgat tgctgctttg gctgttgatt cggatctcta tttatttgat gagcctactt
      661 ctggtttaga tcctttgatg gaaacgattt tccaagaaga agttgaaaaa ttgaaagaac
      721 agggcaaatc catcctactt tcttctcata ttctcagtga agtcgagcga ctagcggata
      781 aagtcgtgat catacgcgaa ggaacactcg atgaagaagg aacactcgat gaattgcgtc
      841 atttgactcg ttcgaccatc actatagaaa cagaagcaga gaatagtcac ttagcacaat
      901 tgaacggtgt ccatgacttt gtgcaaaaag gtcacctcgc tcaatttgct gctgataacg
      961 aagctttcga tgtgatttta gctgaagcgt ccaaattagg tgtgaaaaaa tttgaatcta
     1021 ttccaccgac tcttgaggat ttattcatga gacattatga aggatgagga ggaagcgaaa
     1081 tgactgataa attttcacgc tggtccaccc ttttttctca aaaccttagc cgtgactgga
     1141 aaaagatcct gatatggcta gtcggtgtca ctgctttttc tggtggtttt gtccctgcgt
     1201 ttgaagaatt gacttccgga caaggattag ctggattata cgaaaccttg caaaacccgg
     1261 caatgattgc aatggtcgga ccaactccag cttcgaatgc tgctgagtat actttaggag
     1321 cgatgtatgc aaatgaaatg ttgctttttt gcagtttgtt tgctgctgtg atctctgttt
     1381 tacacgtggt gagccaaacg agaaaaaaag aagaacttgg tctgattgaa cttattcgtt
     1441 cctttcgagt cggacgtcaa gcaaactctt tagctgtcat cgctgaaagt attctgatca
     1501 atttgctttt agcaatatcg atcagctgta cgatgatttt tttccaagcg gattcgatca
     1561 ctgcagaagg tgcttggctt tttgggctat ctgtaggtag tatggggatt ttggggagtg
     1621 ccgttgcact gtttgctgca caactgatgc caagttcagc cagttcgaat ggtctgtctt
     1681 tagcgttttt aggtggactt taccttctac gagcaataac agatgtagct gattcctccc
     1741 tttcgatgtg gaatccttta ggctggtctt atttgaccta tccatttaca actaacaact
     1801 ggattccttt gctttatttg tttttgttca gtctaatctt gataatcagt tcatttgttt
     1861 tagaggaaca tcgagatttg aacaccggct atatccccga acgtgaagga aaaccgtatg
     1921 ctaaacgttc tcttttatcc ttgcgtggtt tattattcta tctaaataaa gggatgacaa
     1981 tcagttggct tatcactttt ggtttgatgg gggcggcata cggctctatc tacgggaata
     2041 tgcaacagtt tttagaaagt aacgacttaa tgaaacagat gtttatccaa tctggcgtct
     2101 caattgaaaa aaactttaca agcacgatca tggtagtctt gatttgtcta gctgctattt
     2161 taccaattgc tagcatcaat cgtttattta cagaagaaac ccacggacgt atgaatcagc
     2221 tgttcgcaac aaaaatttct aggaaccaac tttattggac caatatgtta ctagctattc
     2281 tatttgctat attggcaatc ctgattagtt ctggtgcact agggctttct gctttggctt
     2341 cgatgaagga tgcgaaagac atggatctat tgatgtttct taaagccggg ttcaatttcc
     2401 tgcctgccgt tttagtgttt atcggaatta gtggctttct attcggatgg cttcctcgaa
     2461 tcggtaaact tgcttatatc tacttagctt actcctttgc actcaattac tttggcggaa
     2521 ttttggattt accagaatgg ttttccaaaa cagctgtccc tagctgggtt ccacggatgc
     2581 caaacgattc ttttgatctt tccgtgttcc ttacgttatc atgtatcagc cttgcactgt
     2641 tcgtccttgg ttctattggt tataggcgaa gagacttact agaaggataa taaaaaagca
     2701 tgtacaattt catgctagcc tccaaatctg ggtcaaaaat ccaacatttg gaggtcattt
     2761 ttttatttac aagtcttcta actgctcttt atcaatgatc cagacttctc gattctttct
     2821 ttttacataa ccattctcct ctaataattt aaattttcta gataaagttt cgggggtcgt
     2881 accgataaat gcagcaagtt ctttcatttt cattggcagg aaaaagtgat ccttccctgt
     2941 cgctttagct aagtcaagca gatagacagc gactcgttct tctacttttt ccatcatcaa
     3001 aaaagttgtc tgctgctgta cttttgttaa tttctcaacg ttgattgcta aaagtttcag
     3061 actaagggcg ggataatgca gtaagagttc cgagaaatct ttttgtctca atatgcaaat
     3121 ttccgttttt tctagtgctt cgccaaataa agactcattt ggaacgccta gcaactggct
     3181 ctctccctca tatccgcccg gctctattac tcgtaaaagc tgttcctttc cgctagcaga
     3241 taattgatac actttcatac tgccggaggc cacgatcact aattgaggtt ctccattcgg
     3301 cgcaagaatc tgttctcctt tttccacata gtgatgtttg acaagtgcgt gtactttttc
     3361 ttgatcttgc tgtactaagt gattgaacaa aggaacaaat gaaacacata aatgatcatg
     3421 actcataatc tctcctcctt ctaaacagct atttctaata aagaaacata acttccctta
     3481 ttccaactga taattttctt ttatcatagc ataaacgtca agcgaaccag ataccagcaa
     3541 atagagtgga tttctttttg tcccttgctt tattttcctt cttttgagac cttaagtaat
     3601 tgattcctat caatttttta aatatttact ttttgaataa tcaaaaacaa accttcactc
     3661 gcaagccgag agtaaggttt gtttttcgca ttgcttattt cgtgttgtct attgaaattt
     3721 ttccgcacct agcttttgat atagtggtgc tagttcttgt aaggtttcag cggttatttg
     3781 ctcaacgtgt tcgatttctt gcggatagaa gaatagttta cccatttgaa aggttcggtc
     3841 tctcatcgct tttttcaact cttcttgttt ctttgtatcg gacaaatagt cagacaaagc
     3901 taagtggtcg tcaagttcat gttctggttg cggccatatg taataatcct tgatatcgac
     3961 tgttttgctc cactctttca gtatcgcaag caactggtta tacgctgcaa tgctaccata
     4021 tcgttcttcg cttttataat gttggtacat caaatagatt tggtattgtt tttgattcaa
     4081 caaaactgct agacaggcat tctcgtcttg acgagattcg cttcggtacg cgctccaaaa
     4141 atgactgcgt aaattccagc cattcgtcca gctttcgatt ttaggtttgc tcatgccgta
     4201 catatcaggc aaaagtgcag ctgtctggag ctgaatctct ttccattttt gccagtgttc
     4261 tttatattct ttcttgattt gttcaatctt atctgttgga tggctctctt tgagctgttt
     4321 gaatgcgtag attggtcgat caaacaactg ataatctgat tttccaagtt ccattgtttc
     4381 gcctctttcc gttatctttt gtattctacc tgaattttgc gatatggaca aatagaggtt
     4441 gtgaaaaagc tgttctgcat caagtcataa gaacagccaa acaaaaaact gcgatgagca
     4501 tcaccgcagt tttattgaac ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa
     4561 atggcactag aagcagctcc tgggtcgata tgtccgattg cacgctctcc tagataggaa
     4621 gctcgtcctt ttttagctac caagactttg gtgtgttctt ttgcctggtc aatgatttct
     4681 tttgttaaat tccctgcctt caatgcctct gtgactggca gccaaacatc taacatcgtt
     4741 ttatcgtcag cttctgcctt tcctcggctt tgaatacccg ctgttccctg ctcgatcact
     4801 tcgcctaatt tttcttgaga atcaatagaa tcgacctctt ttgtcgcttt catcatgttc
     4861 atgaatgcag atccgtataa cggaccagaa gccccaccga ctttcgatat catcgccata
     4921 gagaatactt tgaatgtatc gctgattgtt tcaggatgat cggcttgaaa agcttcttca
     4981 taagctgaaa cccctcttcc catgttattt ccatggtctc catcgccaat cggggtatct
     5041 agatcactga gatattgttt gttctgattg attgcctctg caaattgcgt gagccattct
     5101 tggatttctt gaacggttag tttcatatta tcaccctttt atcttgagat tttttaccat
     5161 gaaatcgtct gtacattgct ttctaaagaa tctttccatt gtgtctccgt caagtcgatc
     5221 agtgttagag aaagaccttg catatctagt gaggtcataa aattaccgac ttttcggaaa
     5281 actacattga cgcctctttc ttccaacaga gctaaaacat cattcatgaa cacgaactgt
     5341 tccatcaaag gcgtcccgcc cattccgttg actagtacac ctgcttcttt tggtaaattc
     5401 gagtaatcat ctaaaatctt agtaacaagt tcctttgcca gtactttaga aggctgcatc
     5461 ttttctcgac gatagcctgg ctcaccgtgg ataccaacac catactcgat ctcatcttct
     5521 cctagttcga atcctggttt ccctacttct ggtacagttg ctgctcgaag cgctagaccg
     5581 atcgtcttag ttgcctgtac gaccttttct cccagttctt tcaactcagc tagggaagca
     5641 ccgtttcttg ctgcatcgcc cacgattttg tgtacaagga cagtacctgc aactccgcgt
     5701 tttccagcag tatacgtact attttcaacg gcgatatcat catctacgat cacactttct
     5761 acttcaatat cgtccatagc agctaattcc tttgccatat cgaagttcaa tgcatcccct
     5821 gtataattct taacgatcag caaaactcct tttccactat cggcttcctt aatagccgtc
     5881 tgaatctgat ctggagttgg agaagtaaac acatcaccaa gcaccgctgc acttaacatc
     5941 ccttccccta caaacccggc atgggatggt tcatgtccgc ttcctccacc agaaaccaaa
     6001 ccaacttgtt ccgctttctg attcttggct acaacacggg aagattctac acgatgaaca
     6061 agttctggat agctttttac taatccttcc agcatttctt cgacaatatt gcttggatca
     6121 ttcatgattt ttttcaattt attcccatcc tttctttccg gcattttgtg cttctgttaa
     6181 gatttgctga gtatcatctg ttataccggc agtgatggct gctgcaaaag ctccttccac
     6241 taaaggcgca tccaccagat gataacgttg ctgctgctct tcttccaaca tgtcttttgc
     6301 cagttccgag ttcaataccg cgctaccgat atccgcaaaa acaaaataac tagctgcatc
     6361 gcttgattca ttgactgctt ctacgatttt catcgggtct gatccgatct cgccatttat
     6421 tccgccgaga gaaaaaattt gcacctcttc ggatttttgc atctgctcaa tcatttcttt
     6481 aataccatct gttacttttt gactatgaga aataagcaaa atactttttt tcacaagaat
     6541 cttcctttct tttacatagt tgctaacgat tggtttcgct ttcattaatg aacaattcaa
     6601 gtatataata aatttctaat tttgggaaaa tatgtgtagt gcttcttgaa gtttgttata
     6661 ctttaattaa aagagaggag tgatcgaatg attacaacaa caacgaacag tgtagaggga
     6721 aaagccgtag tagcttataa agggatcgtt tttggtgagg tcattactgg gatccatgca
     6781 tttaaagact tggaggcggg gttgcgaaat atctttggcg gccgctcaaa aagctatgaa
     6841 aacgaactaa tgggtgcaag agaagaagca cttgccgaaa tggccgaacg tgcaaaagca
     6901 ttaggagctg atgcgatcat tggcgtaaaa atggactatg aagtattagg cagcgacaat
     6961 ggcatgctga tggtcacatg tagtggaaca gctgtctcca cgagataagt aggggacatc
     7021 tgttttaata actaaagaga gatgggacat gtagtggcac tcaagaatta gatttttcag
     7081 tctaacttcg aggtactcta ccttcccatc ctctcttttt tatttctttt tgcggatgat
     7141 tccgtaagct gctgcgacta acacacccgc ccctgctaag gaataactga cagaagctgt
     7201 tttttcattt gtttttggca aagtctgact tgttgatttg gatggctctg tcgtttgctg
     7261 aactgtttct gtcgtgcttc cagaggtcac tgatttttct cctgccgaat gcaaaatatc
     7321 ttttattcgt tggaaactag ctgctaagtc agcttttttt gcttcaaaat cattagcaac
     7381 tgtttgtgct tcgctgatct ttccgtggat tgcttgactg gccgctgtga tatccgcttg
     7441 agtttgactt agctttccag aagctgtttg agcaacctga tttaattgct gattgaattc
     7501 ttttgcttgt tggatcttcc cgtcaagatc tgtttggaca gcttccgctt ttgctttcgc
     7561 ttcggcaagc tttttctcaa gttgtgcacg gtcatttccc actttatttt taagatcagc
     7621 taaatcttga tcgattgctg cttggtcttc tttcaatttg ttttttgcag cttctaatgc
     7681 ttcttcgagt tcttttcttt tattttccaa gtcttgcaaa tgatcgattt ggtcagaaga
     7741 ggtagaacta tttattgatg tatcttctga agatgaaatc gtttctcctg aattatctgt
     7801 cggtacattg gaagtcattt ctgtttccga ggcaccgttt tcagcaccag aagagcttat
     7861 ggttgttgaa ggttctgttt gagcaggttc agaagtttcc gcaaaagctg tctgtcccaa
     7921 taagaaaata ctggaaacag caattggcca gattaacggc ttcattcttt ttttcatcac
     7981 ttttcccatc cttactactt tttaactaat ttcttattat aagcaaaaat gtttggaaga
     8041 accataaaca caacaggctg tttgtctaaa aaacggtgca tcttttattt ttttgtacgt
     8101 tccgggcttt tccatgtccc gcttccataa cctaaaatcg ttttgactgc tggaatgaaa
     8161 gtcgtcacga tcgttatcgc gttcactatc cagaacaaaa gcatatatag tggtgcgaaa
     8221 agaaggtatt tcaattttct tcgtcgatca tctacaatca gcgaagctag caactggaaa
     8281 aatcctgcga ccatttcaaa gcagatgaac acaaaactga atgtaaaagt aatataaatc
     8341 tgttcgtagt ttccttgata tacataatgc ccgattaatc cagcaaaaag aactacggaa
     8401 atacaaaaga atattgacca gacaatactc aatgtttgat caatgaagat gatcgttctt
     8461 ccaatatgtt taaaaggatg gagcatcact tttttgaaat tcgtcagcca gacttctgtt
     8521 ccaccttttg cccaacgttt tctgtgtcga tatagcatct tcaacgtcac aggtacttcc
     8581 atgaaaaaga taatctcagg agcaaaaaca gataaccagt catttaactg atgatcccaa
     8641 gcaatactga tgtcttctgt cgctcgatct tgtctgaatc cgcccacatc gatcaaggct
     8701 tcttttcgat acatcgtatt tgctccactg tacgcataaa gaccaccgaa aacagctgac
     8761 tgtgttcgtt taatgatgcc cacgatactt gagaattcaa ccgtttgaga ttttgcaatc
     8821 aatttggtac gattttggac atccatattt gcagtgaccg cagcaatatg tcttgcacca
     8881 gggcgaatga aataattcac atacctaatc agtgcatctg gctccggtac cgtgtctgca
     8941 tcattgctta aaatcaattt cccttttgca aaggcaagtc cgatattaaa cgcgtgggct
     9001 tttcctttat ttttttcgat ccgaaccaca cgaagattag cgtatttttt catcagtcta
     9061 gctaatattt ctggtgtttg gtccgttgaa ccatcgtctg tcactaatac ctcatagttg
     9121 tgataattca atttcgtcat caaatactcg attgtatatt cgatcacaat ttcttcatta
     9181 tgggctggca ccatgatcgt gatgaacggt tcgacactca agggaacatc tacccattct
     9241 ttttgcttat gtttgtataa aaacacataa caccagacac caataaacca tacaaatccg
     9301 cctaaaatcg gataagaaac gagaaaaaaa tacataatag acaaacaagt atctaagaat
     9361 aaattacctg tatgggtgat tgagatcatt tcagttccac cccattttct ttatacaaat
     9421 ctgcaatgaa tgtcgtatct agatttttct cttcggagac agaataaaaa cggaccgttt
     9481 ctctctcttc ttttgttcca aaacgttccg tatacacttc ttcaagtaat tcttgtcttt
     9541 ttttcaattt ttcatcttca tattcaaaag atttatttac ttttttgtat agatttcgat
     9601 tgttccacct agtcaataca aatgaggtaa tcaggaaact gaaaaaaatc aatacaaaaa
     9661 agccgattaa gaaatacagc attttctttt ctgataaaaa agcgtacaag gaaaaatcct
     9721 tggctctttc tggtctcgtt aatgggaaaa agacccatat aaaaggaaga acaacgatca
     9781 tccatcctaa aagagccact aacgtttgcc aaatctttaa ccagtaatta ccttttgaaa
     9841 aatacttatc tttcaccaaa attgatgcaa cttcttctct tgttactttt ttcatatcct
     9901 cacctcacgt ttttttgtta cgacgtagga gtttaccata catttcattt cttttcacat
     9961 aaagtgttcc taaatttttc ctaagaaaaa gaacaaaaca tttctcatca gcaaatgaaa
    10021 aatattttgt tctttttatc agttgttctt tttatttttt atctcttaat tatgttgctc
    10081 tttccaatac gacaatgctt ttcattcctc tagatatcgc cgtgtacatt tgtctgcgtc
    10141 ttctttgctc atctccatcg ttttcaaatg gatataagat tacatgatca aattccagtc
    10201 cttttgctag actcaaagga atgatttggc agttttctaa tccatatgaa ttttttttaa
    10261 tcgattcttg tagcgtttgg gtttcctctt ctgtttgggt gataactgcc gctttttctc
    10321 cttttagaga aaccaaaatc tcagctagta ttttcaacaa ttcttcagag gatcgaaaag
    10381 agcggtattc gggttctttt ccttgcggtc ggatcgatac gatgtcgatc ttctcttgat
    10441 ccacagcata ggttttgaaa agctctgtga ttgctccgct agaacggtaa ctatttctaa
    10501 gatcataccg atggatcggc agatggtagt cgtcgaaaca acgcataata tcagcaaaag
    10561 tagtggaaga attaaagatt gcctgattct cgtcacctac taaagtaaag tgagtttttg
    10621 gaaataattc gatcaataga cctaattgtg ccagggtata atcttgtact tcatcaatca
    10681 atagatagcg aaatgctggt acatttacct tttcaacaag taaatgacga atagctaata
    10741 taatgactgc ttcatcaaca gtacgtgtgg gttgccaagc ataagctgag ccatatcgtt
    10801 tttcataaat tctttcaaac aactgatgct catccaccca tgccatttct tcgatttgtc
    10861 tggttatttt tcgatatttt ttttgtaaaa gtttccttgc ataagctaca atactttgtt
    10921 ccgaatcatc agaaatcaat ttaccgaata attccaactg cctatcttct gtcaacgaga
    10981 gtacctgatt ctgcacagca ggactttttg cttgtttcaa aagatgattc ttccattcgc
    11041 tcatcagtgc ctttttcgtt gcttgaagcc tatcgatcaa tttaggatga gcaggaactt
    11101 tctcatatat tttttcgatc gtttttttag aaaggactgt ttttccattt ttagtaagtc
    11161 cacggataaa attcggatga tctaaaaaca aaggatctga ttgttttaga ttatcgataa
    11221 actttttgct acgcaaacgt tctgtctgct ccgatacatt ttctcccgtg atacgtttga
    11281 aataagcctc ttcaccttct atttcctctg ctgaaagctg tgaaaccagc tgtatgatgg
    11341 ttagattcag tggatttctt tcacctaatg atggtaggac attcgaaata tagtcgataa
    11401 accgatgatt aggtgataga atcagcaagt catcggatgt catcgtatct cgatataaat
    11461 agagcagata cgcaatacgc tgcatgatag cagaggtttt accacttcct gctacgccat
    11521 ttaccaagat attttgactt ttcacatccc gaatgattgt gttttgttcc gattggatcg
    11581 tagcagtgat agccttcatt tcattggtct catcttggcc taagacttct aataatacgt
    11641 catcttgaat agctacagac gtatcaaaaa acttgaccaa acgatctctt tccaaaagaa
    11701 attgtcgtct ttctttaata accgtatcga ttttattttg acgaaccgta taagaagatg
    11761 gaccaatcat gttattgtaa aaaagttcgg caattggtga acgccaatca taaacaagat
    11821 tttcatctgc atcatctgtg aaatttgctg taccgatata aaaggattct tttggctcat
    11881 tatccagaaa gtctacaacg acttttccaa agtagggact tttcaataaa cgttcgatct
    11941 ttgctagctg attttctgcg tttttctgtt tgatattcat ctgatcgatt tctctatttt
    12001 tcatttcaat catcgaaaaa gtatccaggt tatctaagta actgtcaaaa ttcaatcgaa
    12061 gctcgccagc catttctttc aacgactggt ttccttctat ttttgccgta tccatcgatt
    12121 cccgcagttc gtttttttta tcaattagct tttgatacac gttcttcaga tatgcttctt
    12181 ctaactgttt ttgtgtgttg accatacaca ttctcctctt tttttaaagt ggtggaaccg
    12241 attagtttac ctcttttaag agtcgaatgc aagcttttta acgagtatca tttgttagga
    12301 tatatcagtt cttcaggtcg gacagtttga tataaagaaa atgcaccatc aaggtttttt
    12361 acgtggtagc cagactgctt caagattctt tccgctagat agctacgtaa tccgctatga
    12421 cagctgacga tatattcttg ttggctgtct aattcattta atcggtcccg aagttcattc
    12481 aaagggatat gtttggcttc cttgaatttg cccttttcca attcttctgc tgttcgcaca
    12541 tctaataaaa tttttccttt tgctagttct gtcggcaatt catgccattg tacggtttca
    12601 ctaagccctt ctgcaatgtt cattgctgca tatcctagca tgtttactgg atctttggct
    12661 gagccaaatg gcggtgcata cgtcaattct aattccggta aatcgaagat cgtcaaatgc
    12721 cctttgattg ctgttgctaa aatatcgatc cgtttatcca caccttttgc cccaacacct
    12781 tgtgcaccat aaatctcgcc agtctctgga tggaagatca acttcagtaa aatatctgtt
    12841 gcttctggat aatagcttgc atgatctttt cctgatacat gaatgacttc aaaagacagt
    12901 tcgttttctt tggctgttct ttcacttaaa ccggtggatg cggcagtcat tccaaacgtt
    12961 cgtataattg ctgttccaat accgccttga tttcttcgag aaattccgga aatcgtgtcc
    13021 gcaacttgtc taccttgacg attggctggt gaagcaagag aaataagtgc atcttttcca
    13081 gtgatttgct gttttacaac gatagcatcc cccaccgcat aaatatcagg aatattcgtt
    13141 tgataacgtt cgtctactag gatacccccg cgtatgccta attcgattcc agcggtcact
    13201 gctagctggt tatcaggacg aacaccaatg gataaaataa ctaaatcagc agggatggta
    13261 cgcccatcat ccagtcgcaa tcgtttcccg tggtcttcaa atccagcgac agcatgagaa
    13321 gtaatcactt ctactccttg atgagacaat tctttttcta taaaagctgc catttcttca
    13381 tccagtgttg gaagaatatg tggtgcttgt tcgatcacca tgacttctaa acctcttctt
    13441 tttaggtttt ctgccatttc caatccgatg aatcctgcac caacgatcac ggcacgcttt
    13501 ggctgttttt ctaatgcatg catcacttta tcgatatctg gtacatttct aatagaaaaa
    13561 acattgtcgg cttctgttat ccctggaatc gatggaacaa ctggttttgc accaggagat
    13621 aaaaccaatg catcgtactg ttcaatgtgt ttttgaccct catgtacgac ttctaccgtt
    13681 ttgttttcgg gaaagatggc tgtcacttca tgatttggac gcacatctaa acaaaaacga
    13741 gcggataatg attctggcgt ttggacaaga agattttccc gttcactgat ttctcccgaa
    13801 agataataag gcaagccgca atttgcaaaa gaaacatacg gtcctttttc aaaaacaacg
    13861 atttctgcat cttccattaa tcggcgaagc cgtgtagcag cagacattcc acctgctaca
    13921 cctccgacaa taacgatttt catcttattt ccctcctact gttcgatcaa accattgatt
    13981 catgccacca cgtacattca ctacatcata accagatttc tttaattctt tagctgcttg
    14041 tttactcctc atccctgatt gacaaatcac atagacggtc ttttctttgt ctccatcata
    14101 tctgtttata ctttgtaatg gaacattttt agctcctttt atatgcccgc cccggtattc
    14161 tgcaggtgtt ctaacatcaa gtaacgctgg attttccaaa agcttttcct ttaattctgg
    14221 gactgttatc gattttattg attgaaatag caaattcatt ttctgcacct catttacatt
    14281 tttataaata cctcatgggg tatattaaac aggagcttta ttaatatgtc aaccagaatg
    14341 actcttcatt tttttcacta ctttttatat ttgcataaaa taccgataag gggtattatc
    14401 taattataaa aatagaggag aatgtgttat ggagattgat aataaaaata tcctgaaccg
    14461 tttgaaacgt acagagggac aaattcgtgg tatccaaaaa atgatcgaag acgaaaaaga
    14521 atgtatggat gtcatcactc aattaagcgc tgttcgttct agtattgatc gcgtaatggg
    14581 catgatcgtt gcagataatc tgaaaaattg ctttgagaaa cctgatagta atccagaaga
    14641 acaagcggct aaactagaac aagcgatcaa gatgattatt aaaaagtaaa ggaatgattt
    14701 caatgaaagt cgttttccat attgatgaat tagaaaaatg gtcagaaaca gggaaaaacg
    14761 taaaaaacct gataaaagct tctccagaaa cgacgattgt cgtatctgta aatggcatcg
    14821 cgattaccgg ttatctagac tctgccaatg cagaattctt agatttgcaa ggagtcgttt
    14881 ttcacgcttg cgccaatgct atgcgtgcta atcatatttc agaaagttct ttacctgaac
    14941 aagtaatcgt tgtaccagca ggtgtccttg atttagttga actgcaatcc caaggttatg
    15001 cttatatcaa gccttagtca gtagaggttg cgacagaagt gatcagcttc aagaaatttc
    15061 ggcttatttt tcgaagaagc tacttctgga aaaccgtgaa tcaaaggttg tgaaaacgct
    15121 gtcctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg
    15181 ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaat
    15241 cagcggtttg acctaagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg
    15301 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaaggaag agattgtgaa
    15361 aaagtcgctt agtttcgagt attaagaaaa attttgtgaa aatggatttc tacatttttg
    15421 cataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttaagg aagaggttgt
    15481 aaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagct gctcatattt
    15541 tgccgttttt tgagctaata ccgaaggtca gctgattgaa caccgtttac caagaggttg
    15601 tgagcctacc atttaactcc gaatcacaag aaataatttc aaaaacaact tctcctgttt
    15661 ttggtttggt tggcttatta tcgagaagta gctactggga gtctatttat caaaaaaagg
    15721 aatgcgacat aggtcacacc ccttttttat tttctttgaa acatctttcg gtactcgaca
    15781 gcatacaaga caaaagattg taaaagaccg taagtgatga cggcaaaaat gactctcacc
    15841 cacagcgttg gtaattctgg gaaaagcagc aaaaggatca tgccaacata gatgacagca
    15901 gtcgctactt tgccatacca cttcgctcca cttaatttga tatttttaga tagcccgatt
    15961 aatccagcta caaacataaa gccttctttt atcaagaata gaccaaacaa cagccaaagc
    16021 cacggtcgat accatgccat agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac
    16081 ggatcaagca ccttccctaa gtctgttacc tgattatatc tccgagcaat gaccccgtct
    16141 agataatcag acaaacctga gacaacaatg accgctcctg ctatataaaa tgactgtctc
    16201 agcacaaaat aaagataaat cggcagcaaa aaaatacgga atatcgataa aagattcgga
    16261 atcgtttgta tttctttacg ccagtctgtg cttaatttca ttttatcgcc ctacctcctc
    16321 agcactcttt tctttttatt ttagcttaca cggggaataa aaccaatgat ttcactgttt
    16381 ttatttttct ttacagccac tgatattatg agctaatttg agataatcac ttgctgtttt
    16441 catcgtttac tcttctttct tcctgtcgtt tgcgcatttc ttttactagt actttatgag
    16501 ctttttttga tcctcgctct aggtcacgct gccgtttacg tgttttataa tcgataaaca
    16561 gactgtctaa atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc agacgaacac
    16621 gctgacgagg cacgtggatt atttttttgt ctaaatatac atcaatcgta tcttcaccta
    16681 tatcttcata aactaacgct tctttttggt attcattgac aaatatccga tcaccttttg
    16741 caaagagctg gcgttctttt ttattctcct tcctatttgt aactgctgtc ttaggaagaa
    16801 attctttttt ctgtgtccga taggcttttt ccgccaagta acgctctgct tgatggatca
    16861 atttcatcga catatccatt ttatgagcaa tccacaaagc ttggctttct cctgtttctc
    16921 caacccgcaa ctgatattta ggacgaagtg cttcccgatc gaaagccata gcagccggca
    16981 caaaatcttc atgatctccg gcgaatttct tgatttctcc ataatgggtc gtggcaatga
    17041 tcaatgcccc ttgctcatac atagattcca tgattgcgat cgctagtgca gccccttcat
    17101 ttggctctgt tccactgcca atctcatcta aaagtaccaa cgtattcctt tttatctttt
    17161 tcaaaatagc agcgatgttt tgcatatgac cagaaaatgt actcaacgca ttttctaaat
    17221 tttggtggtc accgacatcg acgaaaatct catcaaagac agcaagctct gtcccttctt
    17281 ttgccggaac ttgcataccg aacatcgcca tcaaagtcaa taaaccgact gtcttcaaca
    17341 cgactgtttt gcctccggca tttgctccgg tgataatcaa gcctcgatag tctttaccga
    17401 tttcaacatc caatggtacc gcatgctcag gtaaaaatgg atgtctgcct tgcttgataa
    17461 tgatatgttc cgatttattg attctaggtg tgcttccatt gatttctcgg ctaaattttg
    17521 cccgagcaaa gatgatatct aacaccgtga tcgtttcgat caataaatcg atcacttctt
    17581 cgttttctgc gatagcacca gtcaatgccg ccaggacttg atattcttca ctgatttctt
    17641 cagcttttaa caactgatag tgttcattca atttagaaat cacagttggt tcaataaaaa
    17701 ccgtcgttct tttatttgat tcatcgataa tcgttccatc tattttattt ttatagctgg
    17761 ctttgatagg aatcgtatag tattcgcctt tttgaacaat cattgcttct tggatcattt
    17821 ctttgttttt cggatgacgc aagaatttca gtaattttga ctggatttct ttttcgatta
    17881 gttgtaattg tttgcgtact ttacgcaaat tacgtgacgc gtcgtcactg actttattat
    17941 tttttatttt ctggtcaatc aactcttcta catttatcaa atcaggaaga tgtttgctat
    18001 aagcaaacaa caagggtgtc tggtattgat tcttatcaaa aaactttgtg atcatccgac
    18061 tgcttctaag aaaatccgca tattcgatca aatcggttgg ctgcagaatc aacccttttt
    18121 tgacttgttc tgtcagtgca tcgatcctcg gcaaccccat aaatggaaca tgctgactgc
    18181 tttctaagat caagcgtgct tctcttgttt cttcttgacg atccaagaca gtttgtaggt
    18241 ttgtcgagac cgtcatatcg ctgattcgtt ttttgctata gtttccaatt gctcttgctt
    18301 ggatttcctt tttgatccga tcaaattgta attgactaat catttcgttg tccatattct
    18361 ctccttttct tgaagagata cctgtcccaa aataaataag ggcctagaga taaataaaag
    18421 gatagaacac agcttaaaaa agtcatgtcc tatccttatt agaatacaaa gatattgttt
    18481 tcctgacagg taccgtttca taagcacacc aaataaactt tgtttattgg ctattcgact
    18541 tactgattag ataccagcta cactcacaaa acatacccca ttcttctagg tcttatttat
    18601 tttccactta actatagcaa gacacatttt ggttgtcaaa ctttagtatg ttttatagat
    18661 ttacaccatt aatgaataga cacctaccgc gataattgct ccgatcattg gacctgcgat
    18721 tggtacccat gaatatgccc aatctgaagt tcctttatta gctattggca ataccgcatg
    18781 tgctaatcga ggaccaaagt cccgagcagg gttgattgca tatcctgttg gccctcctaa
    18841 tgaaagaccg atggctaaga tcaatccacc tacagcgaat acgttcgttc caggtgcaaa
    18901 ttcgttttgt ccaaaagcta gcaacccaag aactaataca aatgtaccaa ttaattctgt
    18961 gatcacattt gctggataat tccgtatagc aggacctgtt gcaaacgttc ctaaaatcgc
    19021 gctttcatct ttcgttgcat tccaatgtgg caaataagaa agccaaacaa gaatggcccc
    19081 tgcaaaagct cctaaaactt gcgcaatgat gaaaggaacg acaagattcc aactaattgc
    19141 ccctgtcatc gccatagcta gagagactgc aggattcaaa tgagctgggc ccatgaatcc
    19201 agaaatatag acagccattg ttacagcgag tccccaacca aatgcgatga caatccagcc
    19261 agaagcctgt gctttacttt tgtttaaatt cacagccgca cacacaccat ctccaagcaa
    19321 aactaggatc agtgtcccaa taaactctcc catgatttga gtcatatcac tgttcattta
    19381 tttctccccc tttagttccc gcagatctga ttctgcaatc aatgcttcta aacgtttagt
    19441 ctgttgcgcc ttctcctctt ctgtccattc aaaatatcca gcaattgcat cgatcacagg
    19501 ttgcttgatc tcatcaagct gatctcgttc aaacaacaga tgatttgtac gacgaatgag
    19561 ataatcgcct ggggccaaaa ccatttcttc ttctaaacca taacgtaacc gagctgactc
    19621 agcgagactc aaaccaggat aaggtgccat ttcttttgct aattcaaaga tacgtcgggc
    19681 attagtcccg taaaaatcag cgatataagt agcatcttct tcctctaaac cggctgctac
    19741 tccttctttt gctaattctg tcactgtttc ttctaattta gttggatcga agtttccacc
    19801 tgaaatctga tattttttag agtcgatttc tttcgtctct attccgtatt cttcttttaa
    19861 taattggcgt atcaatcgca aagctccttc tgccatctta cggtaatcgg tgattttccc
    19921 accagataaa gtcagcaaac catctggttc tctttctaaa gaactacctc tggaaatcgt
    19981 agaaggtgct ttttcatcac ggctgttttc caaatggttc aacacgtctt ctacttcagc
    20041 acgagaaact ttgttttcct tatattcact tactgtatcg acaactttat tgaagctctt
    20101 gtctgaaatc gatccattat ctccaccatt gtaatcagaa ccagaattac caatcaacag
    20161 tggacgaagt cctgcccagc ttgcttcgat atcattcaat gtgatatttg cttctggata
    20221 gcgatgattg atcacatcca atagataatc tacatcttct tgtgtgactt tcggatccgt
    20281 aaaatctcct tggtaatccg tatctgtcgt accaaagtaa gtcttgtttt ctcttggaat
    20341 agcaaaaacc atccgcccat cttgctttcc tgtatcgaag tatgtgggtt gcggtacagg
    20401 cagttttttc gcatctacaa ctaaatggat ccctttagtt ggacgcattt taggagagac
    20461 tggacgcgta aaattcaagt ttcgtacttt atctacccaa ggaccactcg tattgatcac
    20521 taattttgct ttgatttcga tcacttcatc tgtcagcagg tcacgagctt tgacgccaac
    20581 gatttgatcg ccatcatata aaaagccaac cgctttcatt ttacttacta gataagcccc
    20641 atcttcggca gcctttttga tattatcaat cactaaacgg gcatcattgt tgcggaaatc
    20701 tagatagaca cccgcgcctt ttagcccttc ttttttcaaa aacggttctc tttccaatac
    20761 ttcttctggt gtcagcgtat aattctcata tttggttcct gtcacatttg ccagtttgtc
    20821 ataaaggtcc attgctactt tgaccgagaa catattgaag gttgttgctc cttcgccctc
    20881 atagattggc aaaagcattg gatctggttt tgggatgtgt ggggcaatac tttgtacgac
    20941 tgcacgttca ccaactgtgt cagctactac ttccacatca aatgtcttca gataacgaat
    21001 accgccatga acaagtttgg tcgagcgaga ggaagtccct tctgcaaaat cctgcatttc
    21061 gatcaatcct gttttgattc ctgatgctgc tgactggact gccacaccgg caccagtgat
    21121 tccgccaccg atgatcaaca gatccaattc ttcttgctgc attttttgaa tgctatcttg
    21181 tcttgtcttg tttgaaaaca tatggatttc ctccttattc aaatgcttgg gtagctttta
    21241 ctgctttttt ccaaccgttg taaagttttt ctcggcgctc ttccttcatt tgaacttcaa
    21301 atagcttgcc agcttcataa aattcgcgga tctcgtctgt atctttccaa taaccgacag
    21361 ctaatcctgc taaaaatgct gcccctagcg ccgttgtttc caagttttct gctcgttgga
    21421 tcggtacatt caagatatct gtttggaatt gcatcagata ttcattgttt gctgcaccgc
    21481 catctacttt caatacagga atcttgattc cagtgtcatc ttgcatcgta tcgatgatat
    21541 ctctcacttg ataagcaatc gactgtaaag tagctttaat gatatcttct tttgtcgtac
    21601 cacgtgtcaa gccaaacata gcccctcgtg ctgcctgatc ccaataagga gcacctagcc
    21661 caacgaatgc aggaacaaca taaacctcat cttcgcttgt agagcgtttc gctgcgtctt
    21721 ctgaatcgct agctttttgc aacatttgaa gtccatctct cagccattga atcgaagaac
    21781 cagccacaaa aatacttcct tctaaagcat agtaaacctt tccatttatg ccgtaaccaa
    21841 ttgtcgtcaa taaattattt ttggataatt gcggtttttc accggtattc atgacaataa
    21901 atgaacctgt accataagta ttcttgacca tgcctggttc gaatgccatt tgtccgaata
    21961 atgcggcttg ctggtctcct gccatccctg aaattggtgc ttcgcttcca taaaaatgat
    22021 aaccttgcgt ataaccataa acttctgagt tagaagtgac ttttggtaac atcactcgag
    22081 gaatatttag taaatctaaa atctcctgat cccaatctag ctgatggata ttgaacaaca
    22141 tcgtccgact ggcattcgag taatcagtaa catgtgcttg tccatcggtc agtttccata
    22201 ctaaccaaga atcgatcgtt ccaaacatca attcgccttt ttcagctcgt tcttgcgcgc
    22261 cttctacatg atcaagaatc cagcgtatct tcgttgctga gaaataggca tcgatcacta
    22321 accctgtttt ttcgtggaaa aattcttgat agccttcatt ttttaagtta tctgcaattc
    22381 cagctgactg tctggattgc caaacaactg catgatagat tggacgtcct gtatcttttt
    22441 cccatattac tgttgtttca cgctggttcg ttatgccaat cccagcaatc tgatttggtt
    22501 tgatccctga ttcgatgaac gctccggcaa taactgactg tacagaattc cagatttcat
    22561 ttgcatcatg ttccacccaa ctttcttgcg ggaaatattg cgtgaactct ttttgagaac
    22621 tgccgatgtg tcttcctttc ttgtcaaaga taattgctcg tgagctagtt gtaccttggt
    22681 caatcgccat gatatacgtt gattctgtca ttgtgattcc ctccattata tgcaaacgct
    22741 ttctttaact actcttatca tactactgtt ttaaaaaaag aaactgtcaa aattttattc
    22801 tttttttctt tttttgacag ttttttatta tgaagctatt ttttaatcaa ttcttttctt
    22861 ttttcagcgt ctctattctt cttttcaatc gaataatgtc gtaaggtgac gcaaattctg
    22921 acagataata ttcacactgt tttttgtttc tctggcaaaa tgtgatcacc aaatcatatt
    22981 ctttaccttc ttcataactt ccaatctcaa taccgccgat tcccctcagt tctctgatca
    23041 gatattgttg aaaagcaata cgataaatcg gcagactttc taaatcgatt ccaatcacta
    23101 ctggttttgc aatataaaaa tcgatcatca aaagaacatt ggcatactgg atcatcaggt
    23161 aactaagctc actatcttca gctcttttcc tcggcaattg ctgcaccgct gtttcctgta
    23221 atttcgccag taactgtgtt aaggaatgcc cgagcatttt ctgctgctgt tccaaaagcc
    23281 gatcacgctc atagacttcg atctttccaa caaagaaaaa ccattcattg ttgatctggg
    23341 aaatttggta gcctactgcc ttttcttctt tgattttcaa acgacgagga cgataatgcc
    23401 ataacatcgt ttcccgaatg taagtatcta aaacagcggt tggtaacttt ttgctccgtg
    23461 tcaaatcata ctgataaaat atttcttcgt ctactatcga aaaagaaaca aaaaagctgt
    23521 aaaacatcat tgtttcgtat acattcagtt ctgctgctgt tcgactaaga tgcatcacga
    23581 tgattggatc gattgcttga taaagccggt ccgactgata gagtaatttc ttttctttca
    23641 aagttgcgta cgtacttttt tcattcaata gtcttttttt cgtaatcccg agccagcaag
    23701 caatctgtgc ttctgctgat gccgtaaacg ttgtttctaa tacacggtcc aatcctagaa
    23761 tgaacggtct tttttctggt gtattttgca aaaaaagcgg tcgctgatct tccgggatat
    23821 attggaatag ttcataataa aaatatcgta tctgtagctc ttctccgtat aattgaccgt
    23881 ttttgatttg taatccaaat tcctctaaga gctggttcaa ttctttgatc tttcgaaaaa
    23941 cagaagactc actgatatta aacgctgtcg ctaatcgaac gatggatact tgctgatgtt
    24001 ctaataaata gacgagcatc ttaaacttca agctttcctg caataaaaac aatagaattt
    24061 tttccaaact tacatcttcg tccaacacaa gcagcgcctg atactcattt cgctgtactt
    24121 ctactttttt ttccatcatt cggccaatca attcaatatc ttccaaatat tgatcaaacg
    24181 aggcttttga cagttggaca tgctctctca aaccattgat agaggctgtt cccccttgca
    24241 gaacaaggtt tcgcaaaatc aggatctgta actgctcttt tctttctaat aatgcttcaa
    24301 tcttcatagg cagtattcgc cacctttgac cacaccatat ctggcacacg gtattctggc
    24361 tggaaaaata gctcatgttc ttctaactgt tcaaacgtcc atccttcttc tactgcaatt
    24421 gtcagttggt caagaaggtg gaacatctct tttttgcttt ggagctgtgc tccaagaatc
    24481 tttttttcag aatcaaatag aaatttgagt ttaactttgc tttgatttat tgctgtaagc
    24541 ttgtagtcta cgtatcctgt ttcaattttt tggggataaa aaatgctctc ttcttctgtc
    24601 aaacctacgc ttcctaagaa atagccaaac cactgattgc cgactggacg ataggttccc
    24661 tgatcctttg tctgtgagcc actaattgtc ttgctgacat ttcgtgctgt acggacagcg
    24721 ttatttacta gagaaacata cattttttca tttgttggac gaaaagttac ttggatcgca
    24781 tcgccaatcg cataaacgtc tttttgagac gtttgcaagt agtcatctac ccagatcgtt
    24841 ccatcatcat tcaatgttaa ttgttcttcc cacatctgat tgtcggggcg agaatgatta
    24901 gccagcaaaa caagatcata gttctccttt cttttttccg tctctacaat agcggtttca
    24961 ttttcggtca aagacacaac ttgttcttct aaaaaaagtg aaagcccttg atttttcatt
    25021 tcctttacta acggttctgt catttcagga tctagataac gaaaaagaat tgactttcgg
    25081 ctttcgtaaa gatggatttg tttttttctt gatgccaacc cttcagctat ttcgattcct
    25141 gcttgtcctg caccaataat tgcaattttc tttgcattca tgatcttttg ctgaagagcc
    25201 gttacgtttt gtatcttttt aactgggtga ataagcgttg attctgcaga aatatttcga
    25261 aacttttgat ttgaaccagt cgctagaatc aaacgatcaa aatccaattt ttttcctcct
    25321 gctaaaacga cttttttttc tttcaatcct attactgtct tttctgtgta aacatggatt
    25381 cgatagcggt cagtcagttc ttctttcgtg atccaagaaa gttgtttttc actggtaacc
    25441 tttcctttta atatcaaatt gaacccgctg ggaatccatc ctaacttcga ttgcttttca
    25501 atcaaatata tttcacttgt tggcatcaat tggcgaagat aaatagccgc atgaatccct
    25561 gcaaacgacc cgccgacaat tacaatgttc atctgcattc ctctttctta taaggtgttc
    25621 tactgctaac ttctcaatca gaaaacacag tattcataac tcgctttcaa ccttatgata
    25681 catagcgatg cagcagaatc aaccctctcc atcataccgt gttctaggtc tgcttctcaa
    25741 taatgtacta gtggacctaa ttgaagagta tcatttctga agagatgttt caaaaaatgt
    25801 actcttataa aagaaaaaaa ctcgcctttt acgtacctct gtccaaatag gaaacagaaa
    25861 agaacgaaaa gacgagtttt agcgtgtgta atgtaatagt tagaatagac acatcattta
    25921 tttttaattc tttaatgctt caacaactgc ttcgcctaat tgatgagcag attgaggatt
    25981 ttgccctgta agaagccgcc catcaatttg aaccacttct gaaaaagctg ctccagactg
    26041 atagtttgct cctttttctt tcaaagcatc ttctgctaag aatggtactt cattcgttgt
    26101 accatttgct tcctcttctt cattactgaa cccagtcact gtacgtccag ctaaaataga
    26161 ttttccagac tcatctgtaa ctggcaacaa tcctacaaca ccgtgacaaa cagctgagac
    26221 cagcccttca tttcgataga tttgtgaagc gactttccca agatctattg cttctggaaa
    26281 atcccacatt gttccgtggc caccagcata ataaaccgct gcataatcat ttgcatccac
    26341 atcatcagga cgtaaggtat ttcccaaggc ccgatttctg aaatcttcat cttcataata
    26401 ggcccaatcg atttcatcca tattcaaaac actttgcggt tctaaaggta catatccgcc
    26461 tttaggactg acaaaatctg catcgatccc attttttact aatacatcat aaaaatgagt
    26521 cagttcactt aaccacaaac ctgtaggacg ctcgatcttt tcgtactttt ctacatttgt
    26581 taatacgatc aatactttca ttttcccatc ctctttctat aaaaattgcg ttattgataa
    26641 cgtgctttca agaaattata taatgggctt ggcaaataaa gctctttata tgcttcttca
    26701 aaaggtttcg ttacaaattc atcctcacgg ttttccaata atttctctgc ccagtcagga
    26761 tcgacgatca tttgctgtcc aattgctgca agttctgcat atttcaatac ctgctctaca
    26821 tcttcgcgat tgcggatccc accgacacca actaaaggga tctttccttg aatcgtgtta
    26881 tggaccactt caagaatcga tttatcgttg tatttatcag aacgagctat tcgatcatac
    26941 atatttaatg aaacatgcag ataatctagt tttgactcac gtaatcgttc aagaagccag
    27001 atcgtgtctt caaaacggat acctggtgtt tcatattctt ctggtgagaa acgataccct
    27061 acgatgaatg gttttttagc atattgttca accgctgaga aaaccgttgt taataattct
    27121 tcaataaaac gataacgatt ttccaagcta ccgccccatt gatcctctcg tctattcgaa
    27181 tgcggagaga aaaattgttg gatcaaatac gtatttgctc catgcaactc gattccatca
    27241 aatcctgatt caatcgctct acgtgtggct ttgccaaatg cttggattgt ttcaacgatt
    27301 tcttcttcac tcattgctcg tggtgtttcc gcatcaggac gttttgctgc aactgcactt
    27361 gcagatacag gctgttctcc agataaagtt gctcgacttg tcattcgtcc accgtggaaa
    27421 atctgaataa tcgcttttgc gccttctcct tggattgctt tcgctagctc acttaatcga
    27481 ggaatcattt catcatgagc gatgctgagt tcacctggcc agccttttcc gatatcttgg
    27541 acatttgcag caccagtaat aacagcacca agacctttag aacgttgttt ataataagtg
    27601 atctcatctt cggtgatcac tccattgaaa aagctgagtt gtgtcgtcat aggcgacagc
    27661 atcaatcggt tcttcaattg aactcctgaa ggtaaagtaa ttgattgatc aaattgtgac
    27721 attgtcattc ctcccataat tcgctctgat taacctatcg agaatcatct atcattaaac
    27781 cactcgatta gttgtacatg caactaattt gcttaaaaaa ttttattttt tttgtaactt
    27841 atctgttgca tcattcattg tctgggctaa ttctttactt gcttcatttc ccaaaactgc
    27901 tttgtatgac tgatgaagtt cttcccagct accatcgact tccttagcaa attggcgtcc
    27961 ttgttctgta agataaacta atgacaagcg accttcgcta actgttttga cgtatccttt
    28021 atattctaat ttttcaacaa aacgagcaat cgttgatggg gcggaataga gcatttgtgc
    28081 aatatctttt tgtggtattc catctttttc ttttaatatc aataaaataa aaccataagt
    28141 tggtgccaga cctgtccttc taaacgcttc atcagctaat ttattcaaca tgcgatctag
    28201 ctttttcacg ctaaagaaaa gacattcatc taacgctttt tcaattggct tttccatttc
    28261 atcccctcca ttggcgtaca tatcatagta tgatttttat tttttgtcaa aaaacacgga
    28321 ataatgtccg cctcctctgt ctagatttga aaaaaagaag tagccatctt caaagaattt
    28381 cggcttgttt tcgacaaaac tccttctgga acaccgtgaa tcagaggttg tgaaaaagtc
    28441 gtttagtttt gagtatgaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa
    28501 tttggcttaa taccgaaaaa ctggcttttg aacaccgttt atatcagagg ttgtgaaaaa
    28561 gccgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat attttgtcgt
    28621 tttttgagct aatgccgatg gtcagctgat tgtacaccgt ttattgagag gtcgtgagcc
    28681 tgccgttgaa ctccgagtca caaggagcaa tttcaaaaaa cagcttctca tgttttatga
    28741 ctatggtgac ttgtgatcga ggagttggca ggagaatact gtttattcgt aaagaagggg
    28801 ctgtgacaag acttttgtca cagtccctta tttcctttta ttataggatt tcgccatttg
    28861 attggatgac ttgtctgtac caatcaaatg attttttctt taaccgtttc tttgtgccat
    28921 tcccttcgtc atcttgatca acatagatat atccataacg ttttgacatt tgtgaagtag
    28981 aagcgctgac gatatcaatg cagccccacg acgtataacc aaataaatct actccatctt
    29041 tgatagcttc cttcatctgc tcaatatgct gtttcaaata gtcaatgcgg taatcgtctt
    29101 gaacggttcc atcttcttct actttgtcta tagctccgag tccgttttca gctatgaaca
    29161 gtggtttttg atacctatca tataattgat tcaaagcaat tctcaaaccc ttaggatcaa
    29221 tctgccagcc ccattcactt gatggaagat acggattttt aattcctcca gttattaagt
    29281 ttccttggac cccttcccaa ttttctggat gagcactgac tgcactagac atgtagtagc
    29341 taaaagaaac aaaatcaaca gggtaggctg ctaagatttc ttcatcacct acttctattt
    29401 ttaagttgac acctttttct tcaaacattc gttgcgtata cgctggataa tatcctcttg
    29461 cttgaacatc actaaagaac aaagaagttc tttgctgctc aattgttttt tgaatatcat
    29521 ccggatcaca agaatacgga acaagaagct gatagctgat catacaaccg acttgtgaac
    29581 ctttgataat ttcatgacaa gcttttgtag ctaaagcact tgctataaac tgatggtgtg
    29641 caccttggta tttcgcttgt tccaaatggg gatggttttc ttcaattact cctacgctaa
    29701 caaaagggtg atgttttaca cagttgatct cattgaaagt catccaatat tttactttat
    29761 ttttatagcg tttgaatact gtttcagcga aacgaacgta aaactgaata agctttcgat
    29821 taggccatcc accatattct gtaaccaaat ataacggcat ttcataatgg gaaagagaaa
    29881 cgactggctc aatcccatat tttagacatt cgtcaaacac ttgatcataa aaaaataatc
    29941 ccgcttcatt gggtgtttct tcatcaccat gaggaaaaat tcttgtccat gcgatagaca
    30001 tccgatagca tttaaaaccc atttcagcaa ataaacggat gtcttcttta aagtgatgat
    30061 agaaatcatt tccgtgtctt ttaggataat aatacttgct gttgggatcc tgcgccgctt
    30121 ttacttgctg atgcgtcatc atatgccatt gatctgtcca tttttcttta ggtaaatcgc
    30181 tgtcgaatgt atacacatca gacacagata atcctttttc atctacatcc catgcacctt
    30241 cacattgatt ggccgctgtc gctccgcccc ataaaaatcc ttctggaaat tttttcatat
    30301 cgatccccct aaacttgcta gttttcttgt ttgattattt ttcttatgac tagtaatgat
    30361 atttttgaat ctactactat ttgtaacaat aaccattgtg gtcggatcat atccctcttt
    30421 gattaattct tgcaaatcta cttccatcaa taaatcgcct ttacgtactt tttttccatc
    30481 agatactgca ctttgaaagt atttgccatt taaattcact gtatcaatac cgatatggat
    30541 cagtatctct gctccttctt cactgactac cccaattgca tgtttcgttg gaaaaactgt
    30601 tgaaacttcc ccgtttacag gactgtagat ttttccgtcc gttggaatga tcgcaaaccc
    30661 ttctcctaaa gatccactag caaatgttgg atcgtttact tcagataaat caattatttc
    30721 acctgctgca ggagtactta acactatatc ctttgaggat tcttcttcga tttcatcctt
    30781 gtagccgaaa agaaaagtta gcatacagga actgatcatc gctaagaaat aactagcaac
    30841 tagccaaaca aatccttcac caatataaat cgggaaagac aaaatacttc ttgtagcaac
    30901 tgccaaagca ccggctcctg cggcaccagc tactccacca aatatagctc cacatataca
    30961 agcaatgtaa aatggtcgtt tatatttaag agtcacacca taaacagcag gttcagtgat
    31021 tccaaataat gctgtgataa atgcagataa agataaactt tttactttct tatttttcgt
    31081 tttcaaaaat accccaagta ctgctcctgc ttgcgcagct acggcagtca tacatgctgg
    31141 acctagcgta tcacgaccgt agaaagaaag attgttggtc attactggta caagtcccca
    31201 atgtaaccca aaaatcacta acacttgcca aaatccgccg ataacagcac cagataaaac
    31261 agggctaaag ttataaacag ctaaatatcc agctgcaatc agctgacttg cataatctgc
    31321 aattggacct acaactaata aagtaattgg gaccatcacg atgatacaaa tgaatggtgt
    31381 caataaattt cgaatacttt cgtgcaagct tttgcgaatc ttaggctcaa tataagaaag
    31441 aacccataca gctaaaattg ctggaataac cgtacttgta taccttgcta gaatcacggg
    31501 catccctaga aatcgcaagg ttatgctatc actatatgca ctgacaatcg taggataaac
    31561 gagagctgct gcaataacca tagcgatgaa acgatctgta ttgaactttt ttgcagcagt
    31621 atatgccaaa aatataggca agaaatagaa aaatgcatca gctgctgcat tcaaaatgat
    31681 ataagttcct gattcagtag ttaataaatt caaactcgta cataaggcga gtactccttt
    31741 gatcaaacca cttcctgcca agacgcctaa agtcggggca aaaacagcgg cgatcatgtc
    31801 tataaaagag tttaaaatgc cttttttctc agttcctctc tttccactag atgcatcatc
    31861 ttctagtttc atttgtttgg ataatacgtc atacacttca tttactgcat ttcctactac
    31921 gacttgaaac attcctccag cttcgactac agtgatcaca ccttctagtt gctctacctt
    31981 ctccttatct gccttcttat tatcttttag ggtaaagcgt aaccttgttg cacaatggta
    32041 aacattgctg acattttctt cgcctccgac aaatcggaca atatctttcg ctaattgtga
    32101 atagtccatt tttacttccc ccttgttaat taatgccgta gcaatccttc ttacaagata
    32161 agtatagttc ttagaaaacg gtttctccat tcaattagta ccccttataa tagcgaacga
    32221 aactgtacaa tagtgtgtat ggtttactta gccgagcaaa tcactcactg cttgaaagaa
    32281 aacaagtata tcgtttctct tatagtcaaa ggagacaaaa aaaacacgct tgatcttcct
    32341 ttgctgatgt ataagtggta tctcaagctg atactctgtg atttttgttt ctacttggct
    32401 tttgataaga cagcacaaca aagtctcggc atttaacgga agcataacta ggtcagaact
    32461 aggagagatt agttgctgaa tggaatctat ctttttatgg atttttattt gattttcttc
    32521 tttgtagtaa tgatcaagca gttttgtttt ctttttttct tcaactatgg tcagcttttc
    32581 acgcaaaatc tgataaatct cccaagattc aaactcatct agaacactag gtagataaat
    32641 caaatcatcc cactcaaaaa taagctcttg ctcagtagtc aaaattgcat catattccgt
    32701 tattttgttt gattgatttt tgaaaagata cactggttct atccggcatt ctttgatcca
    32761 catgttcata ctttgttgaa tctgtttttt tagtgtattc aaaatactaa ttggctggtt
    32821 actaacaatc agtatctccc cttgttttcg aaaaggattc aacgcttcgg caagatacaa
    32881 aacaaaataa gcgctttcag ctaaattggg acgtaattga aaatgtattg ggaagaaacg
    32941 ccgaattaaa taaatttcta aaggataacg ggagatgatc ttttgtacaa aatgattcat
    33001 ggtattatgt ccggcatcca tcctcgtttt catttgtttg atatgggtta gcaaaaattc
    33061 tgtttcatgg aacaataacg aaacaggaag atctaaaaaa cgaataatcg tttgctcaaa
    33121 tgctcttaga ttcttctcta tttctttgtt tttttcatca ttaatcatca aataatttaa
    33181 ctctaatagt cgattttcaa ttgcttgttt ttcttgattg gaaaaagaat agggtagttc
    33241 tgatgataac ttttttgtga gggtctctac catcggaata aataatgtat acctgtttgt
    33301 tttttctaaa agttcttctc ttcgaagaat ggtcaaaacg agataccgag aaaatcgtat
    33361 gaagtcctct cctgaaacaa tataattata tgatataaga acctctttta atgtcttttg
    33421 gacagtcggc atgatattgg aaaaaattgt actgatttgt tgaggcaacc ttgcgtgttg
    33481 gatcaccgtt tctgttccaa caagagataa gaaaattctt tttgaatttt cttcagcaat
    33541 gatttttagt ccttttgtcg acgaaacttc cagttctaat ttaggattgc gctcgatcca
    33601 acgcaaaatc gtttttattt gtaatgatac ggctgttttt gaaagaaaga aaatgtctgc
    33661 tatcttttgc atactgacat accctttttg aaacaataag tacgtcacaa tagttgaagc
    33721 tcggaaatcc atgctggaat agattttatg tttcgtcacc tcttgtatga tttgctgttt
    33781 agtttcagat tcaattgatt ctaaaaaata acctgaacgt ttatttgact ggataatgat
    33841 tttcttctcg tagatagcaa gcatattttt gatttcagtt tggatcgttt tcttgttggt
    33901 acctaaagct tcagctaatt cttcgcttgt tatccaatga ttcttatttc ttaatactac
    33961 aagaatttgg agttgtcgtt tatttaagtc catttgctta cacctcctct ttgttataaa
    34021 catacaggaa aaacctctca aatgacaata gtcacttgca gaggtttaaa aatcatttga
    34081 agaaagatct atattcaagg tttttaccat tagactggat aatttgttta taatattcaa
    34141 aactatcttt tttcaatctc ctaaagcttc cgctcccatc attttgtcta tccacataaa
    34201 taaagccata tcgcttcctc atttcccctg ttcctgccga tacgatatcg attggtcccc
    34261 accataagta accaatgatt tggcaaccat cttttattgc ctcagcaatt tcctgaagat
    34321 gtatttttag atactcacaa cgttttggat cgtggattcg attcaattca tctggtgctt
    34381 catctagtcc aatgccattt tcaactatga ataatggtaa ttcataacgg tctgtcaatt
    34441 cattcatgac ataacgaaac cctagaggat caattggcca actccaaggt tcgggagatt
    34501 tttccttcaa aaagggatta tcgaaaccat ttgctccgcc agtatctacg cgcatttctg
    34561 cttctttttt atagacagcg ctacgataat aactaaatgc aatataatct gccgtgttag
    34621 cttgcaagca ctgttcgtct ccatctaaca ttactggtgc acagtcttct tccttccatg
    34681 tcctgtaaat ataagctgga taatgccctc ggcacatgac atcggagaaa aaccatgaat
    34741 tgcgtttaaa ttgcagtgaa ccaaatacat tctctggatc acagtcatat gcataagtcg
    34801 ctaatgcaga aaagctacac ataataccca tcttagcatt tggatcgatt ttctttaata
    34861 attgagtagc acgggcgttc gctagaaaca tatgatgaca ggcttgataa atcacttttt
    34921 ttgttagtgt agcaatgggt tcaagtttat ctctagcagg gaatgtaacc aatccagcag
    34981 ctgcataagg catacgaaaa gcattattga tctcattaaa ggtcatccaa tatttcactt
    35041 tcccttggta gcgttcaaag acggtctgcg cataaaattc aaaaaagtcg attaattttc
    35101 gattttgcca ccccccatac tctcctacca atgctaaggg ggtttcataa tgactaagag
    35161 taattactgg ctcgatatca tatcgattta atgtattgat tagtctgtca taaaatacga
    35221 gaccagcctc atttggcttc tgttcgtctc ctcttggaaa tattcgtgcc catgagatgc
    35281 ttgttcgaaa agcttttaaa cctaactcct tcagtaaacg aatatcctct tcaaatcgat
    35341 gataaaaatc aatcgcttca tgagaaagat aatggagtga ttcgtctaaa gcaatctccc
    35401 aaatcttttt ttcttcattc cactgaatac taggatgctg gttcatgatc ccgataagcg
    35461 tatctgtcac attaggtaat ttgccgtctt ccaaccatgc accttcacat tgattagcag
    35521 ctactgctcc gccccataaa aaattttctg gaaatatttc ctgatccata actatctctc
    35581 ctcgctatca acaatttctt acttgaagta tagctcgatt catcaaaaca agatattcta
    35641 actatttcgg cagaagagaa gaaaaaaatt gaatgctact cttcattttt aatatttata
    35701 tataacaaat acataagacg atccccgaaa gaaaacactc gttaaccatc taagcaaacg
    35761 atttatttta cttccaacac aaatgcttca taaggtcgta aatcaacttt gccgtctggt
    35821 aactgtttca gaggataatt tgacacaata acccttaaag cttcacgtgg aacagtgaac
    35881 gtctgctttt cactggacat attacaaata acgagccatt gctggtcttc atatatgcga
    35941 gaataggcaa agacatgctc aggcgtcttt tccactaatt catagtctcc ccagacgatg
    36001 atgtcatgtt ttctacgcaa atcgatcaat ttcttgtaca tataaaaaac agaatcttga
    36061 tctgctaacg cttcttctgc attgatttct ttataatttg gattgactcg aagccatgga
    36121 attccagtag taaacccagc atgttcacgg gcgttccatt gcataggcgt cctagcattg
    36181 tctcttcctt tcgcattgat tgattccaag atttcctctt ttgtaaatcc agaagaaatc
    36241 ctttcgttat acatattgat tgtctcaata tcttttgctt ccgcgatatc atcgattggg
    36301 caattcgtca tgcctaactc ttctccttga tagatataag gggtgccttt catcatatgg
    36361 agcaagatag caaacatctt ggcactttct tttcgataca catcgtcatt tccccaacga
    36421 gaaacaattc gcggaagatc gtgattattc caaaataaac tattccatcc ttcatcacct
    36481 aaagatgttt gccatttgga taatactttt ttcagttcag agatactcaa gggtttcaaa
    36541 tcccatttgt ctttgccttc ttgctgatct aatcctatgt gttcgaattg gaagaccatg
    36601 gataattcat ttcttttagg attggaataa agtttagcga tttcggtagt tgctccccat
    36661 gtttcgccaa cagtcatcag atttttgtca ccaaaagttg cttgattcat ttcctgaaga
    36721 tactcatgca gtttaggtcc attacttgtg atttctttat ccggttcttt gccaatcata
    36781 tcgatcacgt ccatacgaaa gcccccaata cctttatcga tccagaaatt catcatttga
    36841 taaatctctt ggcgaacctg tttatttttc caattcaaat caggttgacg cttgctatac
    36901 aagtgcagaa aatattgccc cgttttttca tccaactgcc aagcagaacc actgaatatt
    36961 gagcgcaatc tattaggctc ttttccattg accggatcac gccaaatata atagtcccga
    37021 taaggatttt ctttattttt acgggcctca atgaaccacg gatgttcatc agaagtatga
    37081 ttcaccacca gatccatgat gatacggata tttcttttct ttgcttcttc aatcagttct
    37141 tccatttctt ccatcgtacc gaattctgcc atgattgctt gataattact gatatcatac
    37201 ccattgtcat cattcggaga ctcgtatact ggagaaagcc aaattgcatc aatacctaaa
    37261 tacgccagat aatccaacct ggaaataatc ccccgcaaat ctccaatacc atcttgattt
    37321 gaatcttgaa aacttcttgg atagatttga taaacaactg aatggtgcca ccatttttgt
    37381 tccatgattt tcctcctata aatggatgtt tctatttttt gcttaatcat ttattttagt
    37441 ctctctcact aaaaattgta acgctttcaa atagctattg caccttgtta tcggtaacag
    37501 aataaaacaa aaaaacagct gaggaaattc cctcaactgt tttttgaaag gattgattta
    37561 tgcagggaag aatgctgatt tcacaacttc tttataaacg gtgttcactc agctgtccct
    37621 tggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttcgtttt tttctctaat
    37681 actcaggcca gaacactgat ttcacaacct ctttataaac ggtgttcact cagctgtccc
    37741 tcggtattag ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa
    37801 tactcaggcc agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa
    37861 tcccacaaat acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc
    37921 tgctaacatg gtaattggat agaagataaa acctaatgca atcccatctg aaatactata
    37981 agtcaaaggc atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca
    38041 ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt
    38101 tacttgtgaa gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat
    38161 ccctgtagta atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc
    38221 tacaaatgca ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc
    38281 agaagctaaa gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt
    38341 tgctaatcca actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac
    38401 tacgacccac atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt
    38461 tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac
    38521 cagacctaaa atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac
    38581 aagcaagata gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag
    38641 tgatcctaat cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc
    38701 aaggaaagcg ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat
    38761 cgcatcaatg atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc
    38821 aacgaatacg ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata
    38881 agcaaaaaaa gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac
    38941 ccccattaaa acacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc
    39001 atccattcca gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat
    39061 aaatgttgta aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc
    39121 gaagtaggaa agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata
    39181 aagtccgtta aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa
    39241 aacataagta aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa
    39301 tccctttatc tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt
    39361 taataaagtt tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc
    39421 gtttctgttc ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc
    39481 caaaacctgt atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat
    39541 tctttgctcg aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc
    39601 ttcccattag atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct
    39661 ataacaaaat aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct
    39721 gtgatttctc ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat
    39781 actaaagaag gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg
    39841 aatacgccta attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc
    39901 aattcgattg tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc
    39961 tctacgtcat tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt
    40021 ctatttgatt ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac
    40081 cttgtgtttg atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca
    40141 caaacttttt ttacaaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt
    40201 ttataattct gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa
    40261 tgtttgatcg attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc
    40321 agcattgatg gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcattgttct
    40381 ttgggcttca cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat
    40441 ttcttccgga agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc
    40501 ttgggctttt cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg
    40561 tacgccaaaa gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa
    40621 ttcccgttct tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt
    40681 agtcttgaat gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt
    40741 ttcttctgta aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt
    40801 ttcttcactt tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt
    40861 tgcttcagga aatacttccc agaaattggt atccactatt acttttttca tctgttcaca
    40921 cctcttgatt agaataattg cttgatgaat agatagtctg tttggggatc atctcccata
    40981 taaaaaacat gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca
    41041 tcgttgtgtt cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca
    41101 atagctgtat ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag
    41161 attcgctcga tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt
    41221 agtttcaggt aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta
    41281 tcttttagct cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa
    41341 tgctccggtg aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt
    41401 agtaagtcta catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc
    41461 tttcaataga tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg
    41521 catactttat ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat
    41581 gcgactgcat tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt
    41641 ttagtcggat agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc
    41701 ccattggatt ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt
    41761 tctgctagtt tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac
    41821 tgtcctcttg ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg
    41881 gcaatcattc cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt
    41941 aagactagtt tacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc
    42001 ttttttaata actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat
    42061 agatctggtt tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata
    42121 gctttggcaa ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa
    42181 acttggcctc ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct
    42241 gtggaatatc tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg
    42301 ttaatcctgt ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt
    42361 cagcaaatcc accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct
    42421 tgccatcttt tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa
    42481 tcgttgtgag cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca
    42541 atgaaatcgt ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata
    42601 gaccagcttg agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct
    42661 tcccttcttt tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta
    42721 cggcaaataa atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca
    42781 aaagacattc gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg
    42841 taataagccc caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa
    42901 ttgaggcagg acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct
    42961 cggtcacaag tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg
    43021 tgactcggag ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt
    43081 tttcgatatt aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc
    43141 caatagtgga gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc
    43201 taactggaat ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat
    43261 atgattggtc cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat
    43321 atagctcaaa tgacgatctg attcacgatc acgtttgtac cgacgttgac ataacacttc
    43381 aaagtctgct gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg
    43441 gagtcgtgct ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc
    43501 ttccatatac aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc
    43561 cgcccgttct tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc
    43621 gagcgtgatg gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt
    43681 tcctgtacca ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta
    43741 agttaattgg actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta
    43801 ctgcttcatt tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa
    43861 aggtttcttt agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc
    43921 ggatcccgtt attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga
    43981 taactagggg gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg
    44041 tttttgattt gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg
    44101 tagcttctac aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga
    44161 catggattcc ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt
    44221 gcgcatgggc atattctact actgcttttg tcttcaggat attttctttg aaaggcagat
    44281 gggacccatc atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa
    44341 agtctcgggc gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg
    44401 tcattacatt gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa
    44461 taatgatcgg cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt
    44521 tatgcgtatt aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt
    44581 tcaatgttgt atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt
    44641 acttgttttg caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga
    44701 tcatcaccag cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta
    44761 tacccatctc cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga
    44821 tcgttcacat acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta
    44881 tgcttatctt gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt
    44941 tcggtcgctt ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt
    45001 tcttttttct taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa
    45061 actgggctta agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct
    45121 gtagcattga tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta
    45181 ataaagaaac catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg
    45241 ccaaatatcc ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc
    45301 gctaagccga acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg
    45361 aaacctaaaa tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg
    45421 atcgcatcag gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac
    45481 agtagacgta caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa
    45541 aaggcaaaca tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca
    45601 aataaaacag tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct
    45661 tgcagaaatt cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct
    45721 ttctcaccaa aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga
    45781 cctaaagcaa tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc
    45841 atagcaatgg cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc
    45901 agaccggcaa aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca
    45961 gttaaaaaaa tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg
    46021 gctaagaacg tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg
    46081 atagattcaa tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg
    46141 aaataagtta aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg
    46201 agtgtcttta acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc
    46261 aacatagcaa tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa
    46321 ttgataacgc ccatgttttt accctctttt ctttttgtat ttagataact cccttatctt
    46381 gcagaactgg ggtgattttt tcttcaattt cattttttga aacaatattt ttcaaataga
    46441 tcatcgccgt tttaggatca actgcgaact tttcgaactg cgaacgaaaa ttttctgctg
    46501 tgatgatcac atctggtttc atggaaactg cggaagaaat atcacaatgg tccagtttgg
    46561 cttctactcc taatttgttc aaaacatcct cagcagacat ctgtgctgca aaactacttc
    46621 ctaaacctgc accgcatacg aataaaatgt tcaatttgtt catctttgtt ctcccccgta
    46681 tcatttatta tttctcaact actgaaaaaa gcagttgttt gaaatcttcg atatttgtgc
    46741 attcaatcaa tcgatccaac ttctcttcgt tgttgatcaa ttcaattagt tctttcatga
    46801 tgttcagatg cgagtaggag tctactgctg ccaaacaaaa aataatcttt actggatcca
    46861 tttcaggatt accgaaatcg attggttgct caatcgtagc tacgctgact cctaatttat
    46921 tgaccccatc ttctggtcgg gcatgggcta gtgccatgtg tttgccaatc acgatatacg
    46981 gtccgtaatc attcactgca cgaatcatcg cttcgacata gcgctcttcg acaatgtttt
    47041 ctttaaccaa tggtctagca gctagttcaa tcgattcctc ccaagtagtg gctttttctt
    47101 tgatcatgat attgctgtcc gtcaaaatat cttttaacat aggctggatc tcccttttgt
    47161 tgatttctaa attgtttgtt tcgaataatt tttctaactt gccatagatg attcgatcta
    47221 ctcggccacc actttctttc atgacatcta ttatttgagt gaataactcc gtcgattcat
    47281 gactgttagg ctctaaccgc tgtagttctt catttctttt caaaaagtct tctataacca
    47341 agatattcgt ctcagttaaa atcggatcga caactaaaag cggcttattt cgataattca
    47401 attgaaaggt gctgaaaacc agattgacat ccaatttatc tactaattcg atttcttttg
    47461 aacttaaaac tgcagtaatc tcgatctgag gaaacttttc tttcagattc tctgctaaca
    47521 gatttccagt agccatccca tggttacaga caacgactga tttataaaca taattcaagt
    47581 ctcgcttgat tacactggcg atcgtcgaaa agtggatgac aagaaaagct atctcgtcct
    47641 ccagtatctt gccaccaatt acttcttcga tcgtaggaac aaaatgagtg actgcgcggt
    47701 agattgggaa ataatttgtt cggatgtttt ctttcaatgg attcatgact tggatatgat
    47761 tcttgattct tacaatcagt gcagccatgt gcttatagag attttcgaat aaagcttctt
    47821 cctttagatg gaaaggtacg tgtgtctcgt tctctacaaa ttggatcaat tggatcgtta
    47881 gtaattgagc ttgtacccac tcgattgaat tggagatatc tctagcattg aacgtctcaa
    47941 tagtgaaacg aagataattt ctttctactt gcgggacatt gtccaattga aattcctcga
    48001 tcatgtggtc aataaatgca gaaaagctac tgtcttttat ttctgtattt ttccaattaa
    48061 tggcagagat aaattcatgt ttcctgattc gctggatcca gatcaatgta aaaagcagga
    48121 tttgtttttt ataaatatca tcttcttgtt tcaacaactt attggaatag atctcctgta
    48181 tttttttgat ttccaccaaa ggaaaataat ggagaaagat tttttgagcc ggtgttaatt
    48241 gtgcatcttg attcataaag aagtcggtca ttcccagatt ctgattgata aaatcataga
    48301 tcatcgtacg gatagatcgt tctgaacctt tataaaccag tccttgcttt ccataactga
    48361 cgatctctat cccataactg accagtgttg ctcgtaaccg tctcatatct tcatctaatg
    48421 tacttttaga gatttgaaat tcttcttctt ttttgtttag atggatcggg attcggctgt
    48481 aagcaacggc taaaactaga tcaaaaatac gttcgcgccg attcaaaaag ccgtctttca
    48541 catcttttat ttcagtttct aatcgagctt gcttttcctt ttccagcttc aacaaaaaaa
    48601 ctttgttgcg aattgtacgg atctccggaa gctttttact gctcaaccag atattcgctt
    48661 caagaacttc ctgacggatc gtccgctgac tgacattgaa ctcacttgct agatgttttg
    48721 cttttactgg atattgggct gtcatgattc tgtgaagtat ttcaattgtt cttgttttca
    48781 tagaaatgat ctccttctga tttcaagtat atggttccgc ttacataata aaaagtagat
    48841 atttctgcca cttgaagtgg aaagaaagta agcaaagcaa acaaaaaata agccatatgc
    48901 tttttcatct cctcaacgaa aaagacatac gcctattatt acaaaaattt tgcccatcta
    48961 tacaaaaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca actaaactaa
    49021 aatagtgctt cctatttttc gtttggttgt tcttattatc gcagatgcaa gcttttaaac
    49081 accatttagt ggagaggttg tgacagagat gttcagcctt gagcgataag aagcgatttt
    49141 gaaaaatcgc ttcccatatt ttacaaaatc gtgccttatt gccgataggc tatctctggc
    49201 acactgttta ttcagaaaaa ggagctgcga caaggctttt gtcacaactc cgttcataaa
    49261 tatttcctgt caatccagca taatcggctg aataaaatcg gcttgccatc ttttttcttt
    49321 ctcagaaaaa tcaaaatacc atatctgaga aaaatactgt cgttgtgtct tacttcctga
    49381 gatcaatcgc ccatcttttc gatcgactga ataatcacag catctgaaat caattcggat
    49441 ggcaaaacta ttttcgtgga tctgtcgata atttcctact tcctgaatag ttattttttt
    49501 agtatgattt cttacaccgt ctgctttgtt tttttgcaga actttctgat gttcggcaaa
    49561 cagcttgtct gtataacagc ttctggcttt ttcaggagtt tcttcttccc aagcggattg
    49621 gatcttcatg aatatctttt ggatttccag aaactgtgct ttcttttctt tagccgttcc
    49681 tgacatgcgg gacaatatct ccgctttcag ccttttcttt tgtcttttat tcttgacgat
    49741 agaaaaagca gaaacccctc ctactgacaa aacagctaat gcaagaaatc ccgtttgctc
    49801 ataactagaa ggacctgtac ctggaccata atacccataa gaatctctgc cgtaagaacg
    49861 actgccaaaa taactgcccc catatgaacg accaccaaat gaacctctgc ccgaacttcg
    49921 accgctagat gtacctccac catgaccacc cgcgacagcg aatccttggg tcggcagagc
    49981 aaacaaacat aggcccacta acaaaatgat tgctaaataa ttgatttttt tcattgtctt
    50041 cctcgttatc ttttattaca aaaaactatt tttatgatag ccttgctaaa aggaagagtc
    50101 aataaccaga tgcataaact gtttttttag caattttgtc ttatttagtc attgagtaac
    50161 atcggacaaa tgtatgtgtc ggcagtccat aattcctcta tcttcagcat gaacaagctt
    50221 gcagacaaga gatctgcgct tcctccagga gagatttggt tttcctgaca aaagtgcaac
    50281 agtgctgtaa atggacgatt tggtaaagca ctttgactag cagcatgagc aagtttttgc
    50341 atttccacta acgttttctg atcactgcga tgcaatacgc atgtgtcatc tagcgaactc
    50401 atcaaaacta acaacatatg catttgtttc tgtacatatg aatcattatc tagctcctta
    50461 gctgcttgga ttgcaagcct gatatgcgga taccctaata tcgcttcttc atatgcacca
    50521 tttacgccat atttttgctt caccctatga ccatgagtgg tttttttatg acgatatttt
    50581 gtatcaggaa aagcagcaag cttgctgaca tcatcaagta ttttcgataa agaaaaaggc
    50641 tgttccatac tgattttttg tgcaatgaca ctgatcaata gccccattgc ccaaatcgct
    50701 cctttatggg tattgatgcc atttgtagca atcagcattg ccttttccgc ttttcgccca
    50761 atctcagcaa ttctttctct taactcctga tcaatagcat gatgccatgc tatttgagcc
    50821 atttcttcaa aaaaaggagt tagcgtctta gcagacttaa gaaataaagc atagtccata
    50881 tcggaatgtg cacccgcatc cagtgcatcc actaatccag gttttggact taatgttact
    50941 tctgcgatca atgcttcctc taccaacgca gcgagttgct gagtcactgc tttttttcgt
    51001 tcgtcattta tcatgacact cactccttct tcctactatt acttgctgaa accttttttg
    51061 atagctaccc aaatgatatg aagtaaccag cttaaagcaa atgcacaact tgccgtcatt
    51121 agataagcta caagaatcat gactcctgaa ggtaactgca gccaactatc tttaaacaat
    51181 aataacagta cttggaaaac aaatacattg gctaaatacg tacgataagc ataaacagac
    51241 aaccatttga acaatggcaa ttttggcgaa tcgtttacaa tcattttgga agcgcctatg
    51301 aagacagcaa agatgatgac taaactgtat aaactttgga tcgtattcaa gtaaggtgca
    51361 ttcgcaaagc tcaagtctcc tggatgtgcg agcaatgaac ggacagaaaa gaacgcgata
    51421 actaaggcaa caggtaagaa aagataacgt actctgtcca aatattgata gactttttcg
    51481 tgataaacaa atgcggctac cccataaatc atgaaactag caacaaaccg atctaataag
    51541 taccagctgt catgataagg cccagtcaat acttgtgttt gatagaacag ataccatgcg
    51601 acatataaaa tcgttgaacc aatcaatact ggccatacat attttttatt agcaaatact
    51661 ttgtaaccga gccaaacaaa aaacggcatg aacaattgga tctgcagcat cataacggta
    51721 taccataaat gaggcgctcc atcacctgtg acatatttca gtaaaaagct tgcagtatcc
    51781 gtatacggag tttgttgttg gactgctgga aatagccaaa gataagccgt cgtccataga
    51841 agatacggaa cgactagttc aaaaaacttt tctttcaaaa attcccaata agacaaatgc
    51901 tgacttgttt ttaccatatt ataaacaatt gcaaaaataa acattggtgc tgtgtattta
    51961 acgatgacgt aaaaggtacc aagaaaagct gtatttgttg cgctgtggct gtttgccaaa
    52021 gcaaagctca atatcgtttg catcataaca gccgttacag ctgatacttt ctggaagtca
    52081 cttacgttga ttttgatttt tttgttttca aacatctctt tctctctctt tttctttatt
    52141 accaaagttc tttggttaat ttaggtcctt cgactgtttt caatagaatt tttttctctg
    52201 gctctctcaa atactctttt agtgaaaaac cgccttgccg agtcactact tgggtatcga
    52261 attgctcaaa aaaagcagga tgcgattgaa ttacctgaat tggcaattca atcggtgaat
    52321 ttgcgtacag cagcaaatca aaatcactgg tttcttttac tgccggaatc ccagtcgata
    52381 attcaaatcc taagctgccg ccgattcccc atttacatcc tgataatatg tttctagcag
    52441 catgaaattg ctgataaaca ggataatcca tgattttctc ttgtctatac gattcttgtt
    52501 cgatgatgtc ccacggatga atgacttgtt tgacggcatg ttttgagatt tctgttgacc
    52561 atctctgatt gcgggcaggt ccacgtaaac caatcggtag tatttctgcg tgatccgtcg
    52621 acgcacgacg aactgttccg aacatctttt cagttagaac gacccattca ggcaaggtga
    52681 acccttctat ttcatgttta tcaaaaagga caatatcatg tgcttgaatc attttttatg
    52741 catcccattc ttcgtccatt ttggcacgga ctttatttgt ttcgacacgt ccgaatgttt
    52801 tggctgtttc gtttgtgtat cgatttgtca gcatcgtgtc atttgtattg cggacatctt
    52861 gaatggcttc attgattgct tcgatgactt tgctgacatt ttgttctgaa gcagcttggt
    52921 cggtgatatc ttctaggaaa tgatagatcg ctcctagttt tgtataatta ttgatatcgt
    52981 aagcgatagc tggaactttt tcagcagctg cttcgatttc agcaaccgaa cgttttgtca
    53041 cacgtgcagc actttcttta gacatcgctt ggacggtaat ttctttattg tttaaagcaa
    53101 tcaagcggct tgattgtaat ccatgtgcta aaaatgcacc tgaaatagca tttcctgcaa
    53161 tcaatccaat aactggatgt ccagcttgac ggagttttgc ataagcagca gcactattag
    53221 ccaaagccac atggataccg atcaattctt cgttatagcc gtatgcttgg cttggtacat
    53281 cgatcacaac gatgattggc cgtttcactt ttttgtcttt atcttcttcg taaacgtgat
    53341 ccaaaatttg tcccatgcgg aaaccttcaa ctaaactaac ttcaccgttt cgtacacggt
    53401 acatcgggtt tttaggatca ggtacgattg atgtcacgat ggcttcttct atgaatttac
    53461 ttgatttacc ataatacaca gtactaatag aagaagttgg atttttcatg ccagttagtg
    53521 ccgcaaacca atcgtaacca acacttgctg cagctggttc atttccttct gctacttctg
    53581 gtaagtcaat tgattttgct tgatggttag caattgcttc attgtaatcg tcaatggcca
    53641 ttggttttga aagatctaaa gaatcaagta atgataaata aaatgctgag ctttcactgc
    53701 gatgcgtgtc ttttcgttcg tccattgctg cgatgacagc agctttgatt gcttctacac
    53761 tatcagtcac taattcatca atgatttttg attctacccg ttgacgagat cctaatgtat
    53821 tccaaatcaa gtctttgtca ctagaatcaa attctcgaac gcctgcttct tgttcaataa
    53881 cttctggtcc attcaaaccg actctcgctt tttttgttgc gattaaataa ctcattaatg
    53941 ctgaagtaat cgacataccg ccaaatgatc ctacacgtcc tggaatcaaa ccgatgactg
    54001 gtacatattg ttttagagca atgattacat ttccgatttc tgaaatagat aacagtccgt
    54061 agtttgcttc ttgcaaacga acaccacctg tatctagtac aatgactgga aagatctcat
    54121 ttcctttttt attttcttct aacacatgtt caagtgcagc aacgattttt gcaccggaaa
    54181 cttcaccgat accgccacct tggaattttc cttccatagc gattactact gcttgtttgc
    54241 cattgatcgt gccacggctg acaacgatcc cgtcatcact ttcagggacg attccttgtg
    54301 ctggtaaact tggcgcgatg atttgatcga atggatcaat caattcacgt ccattgtcaa
    54361 gtagagcgtt cactcgttct ctggcgttta attctacaaa actatttttc atctgaaagt
    54421 tcctccattg cttgcgtcaa tcgtaggtac acaactcctg gtgtagcacc gaagtcattg
    54481 atcacgatat ctgctgaaat tggataacga tcaaaaaatc tcgttaatac attgttccat
    54541 acttcgccga agccatcgct tcctgtacaa atttttacag tagattctgt tttttctgtt
    54601 ggaaacatca agatttccaa atctcctgaa ccgaccactc ctacatggat tttcttattg
    54661 atcggctgtt tcgttggata tttataagct aattgttcca tggatctctc ccctttttgt
    54721 aaaacagagc atacacgttc tgcttattcg tctttcttct tccatctggg taaatggcga
    54781 caaaaggttt ttctcgccaa ttttatgacc aagttctgaa tttcattggt ggttcataca
    54841 ggccatctga ccattcgatc aaatcatcga tggtttgtgc agctaataat gaacgtttcg
    54901 cttcactacg tttgatgcct agatcttctg gtaatttcac gatgccggct gcacgcaagc
    54961 tatctagtga ttctttcgtg ctagtcattc caagaggtgt aacaccgcca attgctgcaa
    55021 ttgctgcttg tcgttcttct tttgtttttg ctttgtacaa ataagcaatt ccttcttctg
    55081 tcacgatatg tgtagtatct tcgctataaa tcatcactgg tgcattggct aagcctgctt
    55141 gtttttgaac agctagtgca tctaattctt cgacaaagac tggtttttta tttgaaccat
    55201 atgtttcaac catttgaaca actaattttt ggcctttccc taatggatca tcattgtcgc
    55261 gaagtgattg ccatgctggt gtagaatgac gacgaccgcc aggattatgt cccatgttag
    55321 gcgcaccgcc aaaaccagat aaacgtcctt ttgttacggt agaagagttt ccttcgtaat
    55381 ctaattgcaa ggttgaaccg ataaacatat cgatggcata ttgtcctgca gcttgtgata
    55441 atgttcggtt actgcgcatc gtgccgtctt taccgacaaa aaagacatct gggcgagcag
    55501 caatatattt ttccatacca acttcgccac caaaactatg gatgctttca acccaacctg
    55561 attcgatggc tggaatcatc gttggatgtg ggttcaatac ccagttcttc gcgattttcc
    55621 cttttaaccc tagtgattca ccataagttg gcagtaacaa ttcgatagct gctgtattga
    55681 atccaatacc gtgattcaaa gattgtacgc cgtgttctgc gtagattcct ttgattgcca
    55741 tcattgccat tagaatttgc aattctgtga tgttttgagg atctcgtgtg aataaagctt
    55801 ctaattgata aggtttatct gctttcacga caacgtctac ccagtcacca gggatatcta
    55861 cacgtggtaa agaatcgaca acttcgttga cttgaacgat cacgatacca tctttgaaag
    55921 ctgcagcttc gatgattgtt ggtgtttctt ccgtattgaa acctgtgtac aagttgccgt
    55981 ctttatctgc tttatctgca gctactaaaa caacatttgg aatcaaatcg atgaataagc
    56041 gtccataaag ttccaaataa gtatggatat cacctaattt cattgtgccg tcttcaatca
    56101 tttgggcgac acgcaagctt tgtgcaccag cataagagaa atcgatcttg cttgcgattc
    56161 ctttttcaaa aatattcaaa tgttctggtc gtgagatact agacatgatc aaatgcagat
    56221 cattcacttt ttctgcatct actttttcaa gactttctga aagaaaactt gcttgttttt
    56281 gattgtcgcc ttctaataca actttgtcgc cgggagcaat cagtttttcc attacttcta
    56341 cgattttttc agtttctaca tacttgccat tgagcaagtt gctgattttt tccaatcgct
    56401 gcgctttttt ttcgcgttta gaagcccagt tgcggtttgg tttttccata attagtcatc
    56461 tccatttttg atatagaaac gtggcgtcat caataccatg ttggtctaga ttgatcgtac
    56521 ggatcccttt atctacacca aattttgtat gaatcagctt tgtcaaagta ttgccggggg
    56581 gaaattcaat cgtcagatcc attcctaatt cttttgccaa gtccatcatt tggttccact
    56641 gcacagggaa agctaggttt tccaacaaat ctttacgaat ctcttctgcg tcctgagtgg
    56701 ctcttccatc cgtatttttt atatacaaac atctagcatc agatagatgg acagactgca
    56761 tatacggctt gaatttcttg accgtatcat tcatcaagag acagtgagat ggcacaggga
    56821 ctttcagcag cttcgctgtt tgcgcttggt gccgtttggc ttctgccaac gtaacggata
    56881 aaccttcgat actcccagat aacgtatgtt gcatcggaca attttcattt gacagataaa
    56941 ctggatggtc aggatcaaat gtttttgcta caatttcctc agcttcgctt ctcgtcacac
    57001 ctactactac gcccattgca taaccgcttg gataagcttc tttcattaag actgcgcggt
    57061 gatagactag ttccgctgca ttctcaaatc tcaaagatcg acaagcaaca gctgcacaaa
    57121 aagcaccaat cgaatgtcct gctgcaatat caggctgaat accgtttttt tccatttcat
    57181 ctaaataaaa tactgctttt gtcagtaatg ctagctggat aaaaaccgta tcttgatagt
    57241 tttcttcttt atcaactaac cgataccccg tcacattttc taaacgttcc atgtacattt
    57301 cagggatatc cgctaacatc cctaacttct gactgccttg tccaggaaat aagaaacctg
    57361 tactcatgag cctcgcctcc tcagcactta ctttttccac tgctatctta gtcaatttct
    57421 gctataataa aaaatgccaa aaaaagaaaa aaccataaca aaaaggtatg gatgggatgg
    57481 ataattatga aattgcttca tctgcagtat tttaaaaaag tattcgaaac aaaaaatgtt
    57541 acccaagcag ccaaagaatt atttatttct caacctgcct tgagccgtgc gatcaaacat
    57601 ttggaaaacg aattaggtgt ccctttgttt taccatagcg gcagaaacat tgagacgacc
    57661 gtttatgcag aagaatttta tccatatgca gtcaagacat tagacacatt agaagaagga
    57721 atcaaattat tgtcttcggt caatgagaag atcgttactt ccgtcatatt gtatttagag
    57781 gtagcctctg tttctattcc gaacttggtc cgaattttta tggaaaagca tcctgatatc
    57841 caattaacga tcatgcaaca tcatttacca gatgatatta ccgatcctgt actgtacatc
    57901 acttcggaga tgaagcctgg catgaccaat attcctatca tgaaagaacc tgtttatgtc
    57961 gccattccta agaaacatcc tcttgctaaa aaagaacatc tgcaattaga agatatccaa
    58021 cgcaccccca tcttgatgct gtcaaagagc aacgccttcc gtcatacact ggatgcagca
    58081 cttgaagcga aaagtatcga attgacgatc agttcgacga cagatgatcc tgcgacatta
    58141 cgttctattg tcaagcaagg attagctgtt agctttttcc caaaagtttc ttggtcttac
    58201 gataaaaatg atcctttcgt cttgcgggaa atcaaagatt tgcccttgac acgaacaatt
    58261 tatttatctt ctagcttcac agaagatcat cctttgatca aaatgattgc aaaaacgatg
    58321 cgggaatttt atttaggaaa ataaaaaaaa aagagctggg acaaaattct tgtcgcagct
    58381 cttttttcga acaaacggtg ttccagaagt aatttcttag aaaataagcc gaaatttccc
    58441 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagcta aacatttctg
    58501 tcacaacctc ttctctattg tcctagacat tcattgcgtt gatttaaaac ttccatcaat
    58561 caaatctgct agttcatcta gcttttctat tttacgtaga tcaatagaga tccaatgttc
    58621 tttattcatg tgataggctg gaaaataccc ctcttttcct cgcaaaattc cgccaagttc
    58681 aggatcgacc ttcaaatcaa gaatattgat gatcctatgg tcacttgcat catcatagat
    58741 tttttcttct ggaacagaca tcaggacccc aaaccatttt ttgctttttt gatgtctgaa
    58801 tacgcaataa tcaggaaatt ttttaaaaat atattctgga gacaaattgt atgtttcatt
    58861 gatgtgatcg attagttctt ttttcgttag catctgcttc catctctttc ttattgattc
    58921 gctttattca aagataccac gtaagaatag aaaaatgaat acaagtagtt gctaatcaaa
    58981 tacttcgata tttttctttt ttatcaactc ttaaatgttc aatccgcctg tgttggtctt
    59041 gctaccatct actattaaaa tgtttattat tgtttgccca cctatttttt gattttagtg
    59101 gtatcatttt tcaagttcat gatgaaattc ttatcatcag taaatgctcc agaaaaataa
    59161 acaatgattg aaaaatcagt tgaaacttga ccaccgttta ttttctccgt attcttagaa
    59221 tagaagggag tctgattgat gtattcacta atgaaaaaaa tcattaccga aaaagatatt
    59281 cgacgtcatg tttctttagt agaacaattg ctgaatcata caaagataac cgtaaatgaa
    59341 ttagctgaaa tcatagggac aaccgaacga acgatttttt ctgaccttca atctattcgc
    59401 tctcagctgc ccgaaggctg ggacatcatc agcgatcaag caggaattag tttgcaaaac
    59461 cagcaaaatt tattaacaaa tgacctttgg gagatttttt tcaaacaatc agtcagtgtc
    59521 gaactgctga aaaatctgct ttttacaaaa aaagttgctg tccctgattt tctagccgac
    59581 catgggcttt cttatgggac attgaaaaga catgtgacta aaatcaatca gcgtttagca
    59641 agttacgact tgcagatcga tttgacgaaa tatacagctt gtattcttgg aaaagagcgt
    59701 gcgattcgta ctttttatca tcgattgctg attcctttca ctcataacaa ctattttttc
    59761 gaggattatt cgatccatga atcccattat tttcaatttt taagaaacct cagtcaaact
    59821 gaactagcgg ttgaaacaga agagattttc ggaacatgtt ggttctttat caatacgatc
    59881 cgtataaaag cgaattgccg attagattct tctatccata ccaacagcac actctcctcc
    59941 ctgtatgact ctgcattgaa aaaattgtat ttaacggaag gtatttatct taaagacact
    60001 gagctttctt ttgcttcttt ctgctttctg gaaagttgga attacaacaa taactatgga
    60061 caagaaaccg ctcgctgcct gcatcattct ccatttttag aggtcctcga aacgtttgta
    60121 gaggaactag cttcagagct gtctcttgat caactaaaaa aaacatctct gactgataac
    60181 ttggctctcc tcatattaaa atatcatgaa tcgccgatat tgattgaagc attggaccgt
    60241 caataccatc gttttttaga aacatatgat cagcaatatg cacacttgta tcaacagaaa
    60301 gagaatttac tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa ttactttctc
    60361 caactcttgt ctctactgat ccaacaaacg attttttcta tcaaaccgaa tttgttcaat
    60421 gtctatttta ttttccaagg agaaccttca tggaaggcgt tcttgcagca ggaattaaaa
    60481 gattacttag gaaaacgtgt ctgcttctta cctatcgaac taaccgatct ttccactatt
    60541 tcttttgaaa aaacagatat cttgatttcc aatttccctc tagattctct agatatccct
    60601 attgtataca tttcttctat tccaacgaaa aatgagctca atcgtttgac tgaactcact
    60661 ttcaaaactt ttttatagta acggctagcg actgtattct ctagctgttt tttctttttt
    60721 gcgaaacagt ttgttcatga tccctttttc tatttctaca actaagaata gaagaatcac
    60781 atttgctcca atcaataact gttgtgtcat cgttaaactt actgtaccga tcagctgctg
    60841 gccgactggt agaaaaataa ccatcccttg caataaagca aggataccta atgaaagaaa
    60901 aagcgctttg ttttgcaaaa gaccttggtt caatgacggc ttggacaact ctctgcaatt
    60961 gatcatgtaa acagcttgag ctaagacgat attttgcaga agaatggttt gctgtaagct
    61021 ttgcccttca aactgcatag ccaataaata ggcaggaacc atgatcagta gggagacata
    61081 gatgatccga aacaaatgat aaccagatag gattccttgc tttggatctc ttggtgggcg
    61141 cttcatgatt tctggatctg gcgactcgaa ccctagttca taagataaag tgattgtcgt
    61201 taccatattg acccataaaa tctgtactgg ggacaaaggt agcgggcgat tcagcataag
    61261 tgcccaaatc aagatcaatc cttgagctaa tgcagttggc aagaagaaat tgatggtttt
    61321 ttgcaaatta tccaaaatcc gccggccctc ttttacggct tttgcaatcg tatggaaatt
    61381 atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat
    61441 agcaattcct acatcggatc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat
    61501 tccgacgatt tctccatttt tctgcaaagc tttaacaatt cgcagtttat gttctggtgt
    61561 cgttcgagca aagacatcga ctattggcac ttgctgaatc aactcttcat cagacataag
    61621 atccaaatcg atcccttcta aaactttggc agtatgtttt aagccaattt gttccgcaat
    61681 cgcttttgct gtgtctttgt gatcccctgt aatcattttc actgagatcc ctgcttgttg
    61741 gcattccttt acagctttga ctgcactttc ttttggcgga tcgatcaatc cggcgatccc
    61801 tgcaaaagtt aattgggtta aagtctcatg cgtgagttcc tgtttcgaat ctactttttt
    61861 ataagcaaat cctaaaacac gctgaccttt tctcgcaaat tctgcagctg tctgactcca
    61921 tgcttcttgt tctgcagaag aaagagttga tttagcaaat aatacttctg gtgcaccttt
    61981 gacgtaaatc accgctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga
    62041 actgaacgga atctttgctt ctattttttt caaaggtaga ttggctgttt ctgtgtgtcg
    62101 aatcagcgca acttctgtcg gattgccact taaattttct gttttctgtt cttcttttgt
    62161 ttttaattcc tgacagtttt tcataatgga caacacgcag gcttcttctt ttgcagctac
    62221 atccacaact gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac agatcactgt
    62281 catagaacct aatgtttcta ccgaaggaag tcctttgatg atcgcctttt cttttgccat
    62341 ttcatgtact ccaacggata gaatcatggt caaaacagct ggcaatcctt tgggtaccat
    62401 tgacaccacc aaagcaatcg tagccgaaag aaggatatgc caatccatac catatcgaaa
    62461 tgtcgtaaaa ataactaaga ataggctcaa acaaagaatg ccttgaaaaa tttgtttgtt
    62521 taatcggttg atttttttaa tcagaggcgt cgtttgttgt tcgaccgatt gcaatgcttg
    62581 attgatttta ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc
    62641 agattgtaca agcgttcctg aaaaagccat atttttctgg tcgccgatcg attcttcttc
    62701 tgtcaaaacg gcatgacttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc
    62761 cactaataaa tcatgtgctt cgattaatcg gatatctgct ggaacgacat ctccggcacg
    62821 taagacaatg acatctcctt ttaccagttc tttcgcaggg attttttctt gcatcccgtt
    62881 tgatagtact atcgcttctt gtcccatcag ctgcttcaaa ccattcaatg attcttccgc
    62941 ttttctttct tgccagtaac taacgagtcc attgacgatc actacgaata aaataatact
    63001 tccttcaata tattcactag aagcaaattt caaaaaagag gcaatgatca acacgatcat
    63061 cagcagatca gtaaaatgct ttgctatttt tttccatcgt tttgtctgct gccttgcttt
    63121 tatttcatta tatccttgat cagtcaggcg tttctgtcgt tcttcttgcg acaagccttc
    63181 tgttgatgtt tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct
    63241 atcccctcct gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg
    63301 attatttcag gggggtctga aataattcgt tttgcaaaat gtatctgggc ctaactgata
    63361 tttctattgc ttattctttt atatacaaaa tttaagaata caaaccagat aaccaataaa
    63421 aaaggaataa agcgtgtcgt gctttcgaat aacaagctga aagcaattga agtaaaaaac
    63481 accaacaaag caagatcttt atatctttgt gtagtgttcc gtttcttttc tttaataaat
    63541 tgaacatgtg cacacaagat cgaagaccac acaaatagaa aacaactcgt tgtcatgcct
    63601 gccaaaagct gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca aagaccggca
    63661 aaaaataaca ttcctgtaat taaaataccg tgtaaaggta cgcctttctt agaaagcgca
    63721 caaaaccaat ggggcaaact tttttctttt gctgtttgat ataaaatcct gctagtactg
    63781 tagactgcac tatttgctga ggacaaagaa gcaaccaaaa tcattagatt gacaattgct
    63841 gctccgcctt ggcaatggat cacttgcagc atagtcacta aaggactttc ttttaaatct
    63901 aaacaatgcc atggaacaac aaataaaatc gacaaaatcg ttcctatata gaaaaagaga
    63961 atacgccaag gcagacagtt gattgctttt ttcaaattct tttgcggatc acttgtctcg
    64021 ccaacggtta agccgatcat ttcaattcca gcaaaactga ataaaaccat aggaaatgag
    64081 actaccattc cagcaaagcc aaacggtagt aattcatcga tctgtccaaa attttctttg
    64141 attccgatag actgggtatg aaaatctgag acactcaatc ctatacccaa taagataaaa
    64201 aggaaaatcc ccaagatctt gatgattgag aaaaaagatt ccaattcacc aaaccacttg
    64261 actgcaacaa ggttgactgc cattaagatc attagtgcgc tacctccgct tatcaaagct
    64321 ggtacatgta caaaccagaa atggatatac aatcctattg ctgttaattc cgcaagtcca
    64381 gctccaagcc agcaaagcca ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa
    64441 taatagtcaa cgaaatcact gatagagtta aatgtactat ttgctaaaaa taaatcaccc
    64501 atggcccgca tcaaaccata aatagccaaa ccgcatatca aataaacgag aataacagct
    64561 ggccctgctg atgcaattgc ctttgctgaa cctaaaaata atcctgttcc aattgcgcca
    64621 cctaatgcga ttaactggat atttcgatta gttagagtcc gtttcattgc cggtttttcc
    64681 atcaagttca cctttttctt tttcaagcag catggcgact tcctgttccc aagaaggttt
    64741 ctttattttt tcaatacaga gtggtactac aaacaaaatg acactgatgg ctgtgactgc
    64801 aattagattg atccatgttc cagctttcat cgtcagtccg acagaaaatc cgattgccgc
    64861 aaataaaaca ataacgacag tccaaactcc aagattgctc tttttctctt tttttccaat
    64921 tctaaatggt cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat
    64981 gatgacatag acgatacagt agataacagt agttgaattt acgagcatca aaaatgcttg
    65041 attgacgcca ggaatcagca aataaaaaag tgcaaagatt gagataatga cagactgagt
    65101 caaaataacg gatttggata cgtcaaattg attcgtcttc caaaaattga attttggtgg
    65161 atacgcacct tttctagcac tttctgtcac tgtctttgct ggtcctgagg cccaagcaga
    65221 taattggact gctacaccaa tgaagacaag taaactaaac agattcacaa taagatgtgg
    65281 caaaccaaga atttggcaat aaatcgaaat agattgcgcg atattattca attccatttg
    65341 tccttttggt acgacatttg ctacaactaa agcattcaaa atattgacga aaaatataaa
    65401 aattaacgcg gcaaatactc cgcgaatata cgttttaact ggattttcca atcgagtgat
    65461 atacacagag gacatttcga taccagtaaa aataaacatg atcggcgcaa aataaacaaa
    65521 tgaggatgca gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc
    65581 ggttggtacg atcccaactt tgatccacgc agcaaaccct aataagagca tcatcgtcaa
    65641 aggcacgtac aatcctaacc aaacaccgac ttttccgccg atttttgcca tatcaaattt
    65701 caaattcaaa aaagtgatga tccaataaac gactaaaatg acaactaatg taaatttgct
    65761 gttcaaacca agcggcacat tatcgataat attaccaaac aacggagcga gtgctgaagc
    65821 gaccatcacc atcccaggaa acatttggac ccataacagc catgagacaa cgaaccccca
    65881 tttttttcct agagcattcg atacccaaag ttctggacca cctttttggg gaaacattcc
    65941 agcaaattca ccagagatca atgagattgg aaaagcgtac aataatactg caaccagcat
    66001 gtaaaaaatc atcgtccagc tcgttgctgc tacatctgga atatttcgta cacttgctac
    66061 aagggcgcag gtcattccga taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc
    66121 tttcatcttt attccttctt tcaatagcga catatgtttt tgttttcttt tatttttgtt
    66181 caagaacaga aatgacaagg tttcatcgat tcagtacaaa acccagtatc accgaaacat
    66241 ccattgaacg atcttagtga actgggtttg atacatgaaa cgaacgatcc aacttataga
    66301 acgttttata aaaaagaata tcttggcgag gaatatatga aaaacagggg gaaatacaat
    66361 tgttttgatg aagcaaataa ttgtctgttc tccatatgtc tcttgttatt ctttcacaca
    66421 aaaatcagtt ctattggatg atttcgttta gtttcttttc cattgctttg gcgatttttt
    66481 ttgtataaaa attgaaaatt cgatcatcat tcaaataagg agtcatgata gccgcacgta
    66541 gcagagtgat tttctgttct tttttccatt cttctttcgt tagacccatt ctttcaacaa
    66601 aacgaatcgg agaatcaccg taatcttctt gtgtaaatgt agtatgagaa gtaataaacc
    66661 gttcaccata aacatcgcca tccatataag aagaccgatc gaacattttt tcatttaatt
    66721 cattgatagc gttcaaatct gtacagcctt gttctttgaa tacccaattg accatattaa
    66781 aatcaggatg attcaatgga tagacttcga ttgtttttcc tttaacagta aaagtcagat
    66841 gatcgagaaa ttctctgaat ctttgagctg cctcgattga agccccaatc aattgaccat
    66901 atccggtcac attcaatggc aatacacgat gtgctgtcca gactgcggcg gcggttgctc
    66961 cagcttttga tccttccaaa atataagctc ctaacatatc tggagccttt actgattttt
    67021 caaacacata tggcgcgaaa taagaaatga tatttctcat atttttatgt ttgattacga
    67081 ttcctccagc agcataaggg acatagccca tcttatgagg atctatcgtg acagaatccg
    67141 cttctgaaat agctctgaac ccttcatata cctctttgtc aatcgttacg cagtggtgga
    67201 aaacatgatg ttcttcaaag aattcagcta aggaagcata cggcacaaat tctcctgctt
    67261 cgtttagaaa cagtgaacgc gcatagccgc catatgcggc atcaacatgt aaatagaaat
    67321 agatcccttc atcttttaac ctttctctta attggacgat tttatctaca ctgtctactt
    67381 gaccttcttc tgtcgttcct acgacagcaa ccacacctag aattgggatt ttttgtccag
    67441 ctaattcacg aattgttttt tccaaagcat taatatccat tcgataatcc tcttgaactg
    67501 ggatagcaac catctgatct aagccgactc cgcaaatatc taacgccttc atccaagaat
    67561 agtgttttgt ttgagggacc agccatttcc ctaatctttg gatatttttt ccgctacggg
    67621 aagaagctgc tttgacctca tccatttctt cgtccgtaaa gctttcggtc atttccaaaa
    67681 tttcttcaac agataaattc aaaagttccc actcagacat ttttttcact ttttcaggca
    67741 gcacttcctt gacagctaaa ggaatcgatt tgatgcaacg ggcgtaccat aatccttcaa
    67801 gattagcaat agaaccatct gctgcaatgt gtccccatcc gtctgtcata ttgaataatg
    67861 aagcaaaatc ctgtcctact tctgcttcca tttgcgaagt tgccatagaa gattccaatg
    67921 caacattatt tgggttccac agcatcgcat agttgtaagc taataaagca ggcatcaatg
    67981 tttcagcatt catttgtccc cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc
    68041 gtaatcgctg actgacttca tccaagacct ctatcatatg gtcgcgggtg gctagataat
    68101 ctggagtgag cttatctcct tcgctaattg caggcatatc tgaaggaata taattttctc
    68161 tccatccaag atgttcatct accatcttgt tcaacaacat tttataaact ggtccatttt
    68221 cagctttgtc gcctataaag acggccttga tatccatatc tttcattttt cttccctcca
    68281 tattttgttg gtcaatgtct tgcagataca tgtgtttatc tgtaacacct cttcactata
    68341 gcgccaaaaa tgagaaattt ttttttataa catcaaatcc acgtagattt ttacaaacaa
    68401 aaataaataa agtcggattt ttctgataat tcaacgcaaa aaagcgaaca atttttcaat
    68461 aaatcagtct tctaaaatag ataacctaaa aaaagcgaac aaacaaa
//
LOCUS       DAHHPA010000003        61016 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.003, whole genome shotgun sequence.
ACCESSION   DAHHPA010000003 DAHHPA010000000
VERSION     DAHHPA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.003"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(13..594)
                     /locus_tag="NBH55_000150"
     CDS             complement(13..594)
                     /locus_tag="NBH55_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1508168.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(727..1410)
                     /gene="rpiA"
                     /locus_tag="NBH55_000151"
     CDS             complement(727..1410)
                     /gene="rpiA"
                     /locus_tag="NBH55_000151"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCD1508169.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1729..2142
                     /gene="rpsL"
                     /locus_tag="NBH55_000152"
     CDS             1729..2142
                     /gene="rpsL"
                     /locus_tag="NBH55_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCD1508170.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2226..2696
                     /gene="rpsG"
                     /locus_tag="NBH55_000153"
     CDS             2226..2696
                     /gene="rpsG"
                     /locus_tag="NBH55_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCD1508171.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2790..4877
                     /gene="fusA"
                     /locus_tag="NBH55_000154"
     CDS             2790..4877
                     /gene="fusA"
                     /locus_tag="NBH55_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCD1508172.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5049..6236
                     /gene="tuf"
                     /locus_tag="NBH55_000155"
     CDS             5049..6236
                     /gene="tuf"
                     /locus_tag="NBH55_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD1508173.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            6643..6951
                     /gene="rpsJ"
                     /locus_tag="NBH55_000156"
     CDS             6643..6951
                     /gene="rpsJ"
                     /locus_tag="NBH55_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCD1508174.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            6982..7611
                     /gene="rplC"
                     /locus_tag="NBH55_000157"
     CDS             6982..7611
                     /gene="rplC"
                     /locus_tag="NBH55_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCD1508175.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            7639..8262
                     /gene="rplD"
                     /locus_tag="NBH55_000158"
     CDS             7639..8262
                     /gene="rplD"
                     /locus_tag="NBH55_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCD1508176.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            8262..8552
                     /gene="rplW"
                     /locus_tag="NBH55_000159"
     CDS             8262..8552
                     /gene="rplW"
                     /locus_tag="NBH55_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCD1508177.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            8591..9424
                     /gene="rplB"
                     /locus_tag="NBH55_000160"
     CDS             8591..9424
                     /gene="rplB"
                     /locus_tag="NBH55_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCD1508178.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            9467..9745
                     /gene="rpsS"
                     /locus_tag="NBH55_000161"
     CDS             9467..9745
                     /gene="rpsS"
                     /locus_tag="NBH55_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCD1508179.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            9768..10115
                     /gene="rplV"
                     /locus_tag="NBH55_000162"
     CDS             9768..10115
                     /gene="rplV"
                     /locus_tag="NBH55_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCD1508180.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10129..10785
                     /gene="rpsC"
                     /locus_tag="NBH55_000163"
     CDS             10129..10785
                     /gene="rpsC"
                     /locus_tag="NBH55_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCD1508181.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            10788..11222
                     /gene="rplP"
                     /locus_tag="NBH55_000164"
     CDS             10788..11222
                     /gene="rplP"
                     /locus_tag="NBH55_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCD1508182.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            11212..11400
                     /gene="rpmC"
                     /locus_tag="NBH55_000165"
     CDS             11212..11400
                     /gene="rpmC"
                     /locus_tag="NBH55_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCD1508183.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            11425..11691
                     /gene="rpsQ"
                     /locus_tag="NBH55_000166"
     CDS             11425..11691
                     /gene="rpsQ"
                     /locus_tag="NBH55_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCD1508184.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            11749..12117
                     /gene="rplN"
                     /locus_tag="NBH55_000167"
     CDS             11749..12117
                     /gene="rplN"
                     /locus_tag="NBH55_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCD1508185.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            12154..12462
                     /gene="rplX"
                     /locus_tag="NBH55_000168"
     CDS             12154..12462
                     /gene="rplX"
                     /locus_tag="NBH55_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCD1508186.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            12490..13029
                     /gene="rplE"
                     /locus_tag="NBH55_000169"
     CDS             12490..13029
                     /gene="rplE"
                     /locus_tag="NBH55_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCD1508187.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13048..13233
                     /locus_tag="NBH55_000170"
     CDS             13048..13233
                     /locus_tag="NBH55_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCD1508188.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            13269..13667
                     /gene="rpsH"
                     /locus_tag="NBH55_000171"
     CDS             13269..13667
                     /gene="rpsH"
                     /locus_tag="NBH55_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCD1508189.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            13699..14235
                     /gene="rplF"
                     /locus_tag="NBH55_000172"
     CDS             13699..14235
                     /gene="rplF"
                     /locus_tag="NBH55_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCD1508190.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            14396..14752
                     /gene="rplR"
                     /locus_tag="NBH55_000173"
     CDS             14396..14752
                     /gene="rplR"
                     /locus_tag="NBH55_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCD1508191.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            14773..15273
                     /gene="rpsE"
                     /locus_tag="NBH55_000174"
     CDS             14773..15273
                     /gene="rpsE"
                     /locus_tag="NBH55_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCD1508192.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            15288..15467
                     /gene="rpmD"
                     /locus_tag="NBH55_000175"
     CDS             15288..15467
                     /gene="rpmD"
                     /locus_tag="NBH55_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCD1508193.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            15511..15951
                     /gene="rplO"
                     /locus_tag="NBH55_000176"
     CDS             15511..15951
                     /gene="rplO"
                     /locus_tag="NBH55_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCD1508194.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            15951..17246
                     /gene="secY"
                     /locus_tag="NBH55_000177"
     CDS             15951..17246
                     /gene="secY"
                     /locus_tag="NBH55_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCD1508195.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            17308..17955
                     /locus_tag="NBH55_000178"
     CDS             17308..17955
                     /locus_tag="NBH55_000178"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCD1508196.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            18146..18364
                     /gene="infA"
                     /locus_tag="NBH55_000179"
     CDS             18146..18364
                     /gene="infA"
                     /locus_tag="NBH55_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCD1508197.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            18397..18513
                     /gene="rpmJ"
                     /locus_tag="NBH55_000180"
     CDS             18397..18513
                     /gene="rpmJ"
                     /locus_tag="NBH55_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCD1508198.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            18531..18896
                     /gene="rpsM"
                     /locus_tag="NBH55_000181"
     CDS             18531..18896
                     /gene="rpsM"
                     /locus_tag="NBH55_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCD1508199.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            18924..19313
                     /gene="rpsK"
                     /locus_tag="NBH55_000182"
     CDS             18924..19313
                     /gene="rpsK"
                     /locus_tag="NBH55_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCD1508200.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            19393..20331
                     /locus_tag="NBH55_000183"
     CDS             19393..20331
                     /locus_tag="NBH55_000183"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCD1508201.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            20359..20739
                     /gene="rplQ"
                     /locus_tag="NBH55_000184"
     CDS             20359..20739
                     /gene="rplQ"
                     /locus_tag="NBH55_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCD1508202.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21011..21850
                     /locus_tag="NBH55_000185"
     CDS             21011..21850
                     /locus_tag="NBH55_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCD1508203.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            21826..22695
                     /locus_tag="NBH55_000186"
     CDS             21826..22695
                     /locus_tag="NBH55_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCD1508204.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            22685..23482
                     /locus_tag="NBH55_000187"
     CDS             22685..23482
                     /locus_tag="NBH55_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD1508205.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            23685..24431
                     /gene="truA"
                     /locus_tag="NBH55_000188"
     CDS             23685..24431
                     /gene="truA"
                     /locus_tag="NBH55_000188"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCD1508206.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(24886..27555)
                     /locus_tag="NBH55_000189"
     CDS             complement(24886..27555)
                     /locus_tag="NBH55_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCD1508207.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(27793..27981)
                     /locus_tag="NBH55_000190"
     CDS             complement(27793..27981)
                     /locus_tag="NBH55_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCD1508208.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            28251..28613
                     /locus_tag="NBH55_000191"
     CDS             28251..28613
                     /locus_tag="NBH55_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1508209.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            28665..30347
                     /locus_tag="NBH55_000192"
     CDS             28665..30347
                     /locus_tag="NBH55_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCD1508210.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            30464..32500
                     /gene="recG"
                     /locus_tag="NBH55_000193"
     CDS             30464..32500
                     /gene="recG"
                     /locus_tag="NBH55_000193"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCD1508211.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            32549..33550
                     /gene="plsX"
                     /locus_tag="NBH55_000194"
     CDS             32549..33550
                     /gene="plsX"
                     /locus_tag="NBH55_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCD1508212.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            33672..33914
                     /gene="acpP"
                     /locus_tag="NBH55_000195"
     CDS             33672..33914
                     /gene="acpP"
                     /locus_tag="NBH55_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCD1508213.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34182..35186
                     /locus_tag="NBH55_000196"
     CDS             34182..35186
                     /locus_tag="NBH55_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1508214.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35187..36131
                     /locus_tag="NBH55_000197"
     CDS             35187..36131
                     /locus_tag="NBH55_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1508215.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36131..37093
                     /locus_tag="NBH55_000198"
     CDS             36131..37093
                     /locus_tag="NBH55_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508216.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37120..38034
                     /locus_tag="NBH55_000199"
     CDS             37120..38034
                     /locus_tag="NBH55_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508217.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38060..39841
                     /locus_tag="NBH55_000200"
     CDS             38060..39841
                     /locus_tag="NBH55_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1508218.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40189..40875
                     /locus_tag="NBH55_000201"
     CDS             40189..40875
                     /locus_tag="NBH55_000201"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCD1508219.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            40895..44476
                     /gene="smc"
                     /locus_tag="NBH55_000202"
     CDS             40895..44476
                     /gene="smc"
                     /locus_tag="NBH55_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCD1508220.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            44485..45294
                     /locus_tag="NBH55_000203"
     CDS             44485..45294
                     /locus_tag="NBH55_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1508221.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            45305..46303
                     /gene="ftsY"
                     /locus_tag="NBH55_000204"
     CDS             45305..46303
                     /gene="ftsY"
                     /locus_tag="NBH55_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCD1508222.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            46490..48757
                     /gene="gshAB"
                     /locus_tag="NBH55_000205"
     CDS             46490..48757
                     /gene="gshAB"
                     /locus_tag="NBH55_000205"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCD1508223.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(48846..49298)
                     /locus_tag="NBH55_000206"
     CDS             complement(48846..49298)
                     /locus_tag="NBH55_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCD1508224.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            49448..50398
                     /locus_tag="NBH55_000207"
     CDS             49448..50398
                     /locus_tag="NBH55_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD1508225.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            50391..51350
                     /locus_tag="NBH55_000208"
     CDS             50391..51350
                     /locus_tag="NBH55_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD1508226.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            51347..52102
                     /locus_tag="NBH55_000209"
     CDS             51347..52102
                     /locus_tag="NBH55_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1508227.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52125..53078
                     /locus_tag="NBH55_000210"
     CDS             52125..53078
                     /locus_tag="NBH55_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1508228.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(53722..54018)
                     /locus_tag="NBH55_000211"
     CDS             complement(53722..54018)
                     /locus_tag="NBH55_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCD1508229.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            54373..54729
                     /locus_tag="NBH55_000212"
     CDS             54373..54729
                     /locus_tag="NBH55_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCD1508230.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            54739..55638
                     /locus_tag="NBH55_000213"
     CDS             54739..55638
                     /locus_tag="NBH55_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCD1508231.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            56155..56886
                     /locus_tag="NBH55_000214"
     CDS             56155..56886
                     /locus_tag="NBH55_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCD1508232.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            57031..59058
                     /locus_tag="NBH55_000215"
     CDS             57031..59058
                     /locus_tag="NBH55_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCD1508233.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            59166..60263
                     /locus_tag="NBH55_000216"
     CDS             59166..60263
                     /locus_tag="NBH55_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCD1508234.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            60570..>61016
                     /locus_tag="NBH55_000217"
     CDS             60570..>61016
                     /locus_tag="NBH55_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508235.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYVSVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        20198 a        10916 c        13036 g        16866 t
ORIGIN      
        1 cgtttttagg tattagatgc ataattgctc gatttctttt tcaggtaaat tgatatattt
       61 ataacatgtt ccaactgaaa caccgcattt tgaagaaata aattggatcg tttctttttt
      121 ttcataatag agacgtcgga tttttttgat cgtttttgca tcgatttttg gtcttccgcc
      181 gatttttcct tttttacgag cttcatctaa tccaactaat gtacgttctt tgatcaatgc
      241 gcactccatg tttgctaggt tttccattaa ttggaaatag atttttccca tttcgcgcgt
      301 atcgattcct tcatcaagac taatcaagtg cagcccgcga tctttgaata attgcgtgag
      361 ttctgttaat tgccgcgtag atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc
      421 gccttgttgc atgtcagcga tcactgactc caaatgaaca gcagtagaat cattttgttc
      481 cacttcatat gtctcatgga aaatttcttg acaaccaaag tcagataatt gatcaacctg
      541 agttgttaaa tcaacgtcat ttatggttgt gcgtgcataa ccaataattt tcatggtgat
      601 tcctttctag cagtgatttt ctaactggat ataacagtaa aaatgtagca taaagtacgt
      661 caaaaagata gttttttcgc aaattttccg ctgtcttttt ttattggatg atcaagcagt
      721 ttttctttat ttacgacgga ttgcgtcaag aatctcaacg cctttttctg agccgacaac
      781 tactttagaa accatgttta ggaaaagccc atgttctact accccaacaa gttcatctaa
      841 ataggtagct aagctttctg gctgttcgat gacttctaga tgcagatcaa tgatgtaatg
      901 accaccatct gtggttaaga cttctccgtt ttcgtctgta cgcaagacgg gttggaatct
      961 tttttcttca aaaatatgaa gtagctgttg ggagccataa gggatcactt caactgggag
     1021 tgggaacttc ccaagtttat cgactaattt agaagaatca acgatccaaa tcgtttcttt
     1081 tgagtacgta gcgactattt tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg
     1141 aaaatcagca ctgatttcat cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc
     1201 aatactcttt aacggtattc ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt
     1261 aactcctgtg atacgtaaac cttcttcacg catacggcga ccaatctctt ctaccatata
     1321 ataggcggtt gaaccggtcc cgagacctac gatcatcccg tcctttacga atttcgcggc
     1381 ttcaatgcca gctaattgtt ttaaattcat ctttgcgtcc accttttttt agtttgagca
     1441 tcgtgctttc ctcactattt tagcgtgatt tacggtaaaa agataccatt cttttttatt
     1501 ccaataaagc tttttcaaaa aaaattgcat ctcttgttga catgtttttt cattcgtgat
     1561 attctaacaa aggtgcttta tctacaggtt tctgtgaaga aacgtgctga ctgtttatca
     1621 aagcatattt gataaggatg cagacattgc atttattttt tatcacaaaa aaagaaccac
     1681 ctggatgtgt ggaactagac gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt
     1741 aaccaattag tacgtaaacc tcgtaaatca aaggtggaaa aatctaattc acctgctttg
     1801 aacaaaggat ataacagctt taagaaaact caaactaacg taaactcacc tcaaaaacgt
     1861 ggggtagcaa ctcgtgtggg tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa
     1921 tatgcacgtg ttcgtttgtc aaacttaatt gaagttactg cttatatccc agggattggt
     1981 cataacttac aagaacacag tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca
     2041 ggggtacgtt accatatcgt tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa
     2101 caaagccgtt ctaaatacgg aactaaaaaa cctaaagctt aattttaatt attaaacaaa
     2161 tgaaacttgc ttctactata tagaagtaat tactatgaac aatatttcgg aaggaggagt
     2221 tacggatgcc acgtaaaggt cctgttgcaa aacgtgatgt tttaccagat cctatttata
     2281 actcaaaatt agtaactcgc ttgatcaacc gtgtaatggt tgacggaaaa cgcgggattg
     2341 cagctaacat tatctataat gcctttgact tgatcaaaga atctacaggt aacgatccat
     2401 tagaagtgtt tgaacaagca atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc
     2461 gtgttggggg ttcaaactac caagtaccag ttgaagttcg ccctgaacgc cgcgttactc
     2521 taggtcttcg ttgggtagta aactacgctc gcttgcgcgg tgaacacaca atggaagaac
     2581 gcttagcaaa agaaatcatg gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg
     2641 aagacactca taaaatggct gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt
     2701 ctctctgttt ggtcggcttt tataagtcga cctcagatag tgtccttatt tttagcaact
     2761 agatagtaaa gagaggagaa acaaggagaa tgaccagaga attttcttta caaaacactc
     2821 gtaatatcgg tatcatggcc cacatcgatg ctggtaaaac gactgctact gaacgtatct
     2881 tgtactatac tggtaaaatc cataaaattg gtgaaactca tgaaggagct tcacaaatgg
     2941 actggatgga acaagaacaa gaacgtggta ttacgatcac ttctgctgct actacagcac
     3001 aatggaaggg tcaccgaatc aacatcatcg acacaccagg acacgtagac ttcacagttg
     3061 aagttgaacg ttcattgcgt gtacttgatg gtgctgtaac tgttcttgat gcacaatcag
     3121 gtgttgaacc tcaaactgaa acagtttggc gtcaagcaac aacttatggt gtacctcgta
     3181 tcgtatttgc aaacaaaatg gacaagatcg gtgctgactt cttatattca gtatcaactt
     3241 tacatgaccg tttgcaagca aacgctcacc caatccaatt accaattggt gcggaagacg
     3301 acttcacagg aattatcgac cttgtaacaa tgaaggctga aatgtataca aacgatcttg
     3361 gaacagagat cgaagaaaca gaaattcctg aagaataccg cgaattagct gaagaatggc
     3421 gcgaaaaatt ggttgaagct gtagctgaaa ctgatgaaga acttactttg aaatacttag
     3481 aaggtgaaga aatcactgaa gctgagttaa aagaaggtat ccgccgcgca acagttaacg
     3541 tagaattcta cccagtacta tgtggttcag catttaagaa caaaggggtt caattactat
     3601 tggacgctgt tcttgattac ttgccatcac cgttagatat cccagctatc aaagggattg
     3661 accctaaaac tgatgaagaa gtagaacgtc ctgcagacga cagcgctcct ttctcagctt
     3721 tagcatttaa agttatgact gacccattcg taggtcgttt aactttcttc cgtgtgtact
     3781 ctggtgtttt acaatcaggt tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg
     3841 ttggtcgtat cctacagatg catgccaact ctcgttcaga aatctctgaa gtttacgctg
     3901 gagatatcgc tgctgctgtt ggtttgaaag acacaactac tggggacaca ctatgtgatg
     3961 aaaagagtct agttattctt gaatccatgg aattcccaga accagttatc caagtcgcta
     4021 ttgaacctaa atcaaaagct gaccaagata aaatgggtgt ggctttacaa aaactatctg
     4081 aagaagatcc tactttccgt gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg
     4141 gtatgggtga acttcactta gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg
     4201 aagctaacgt aggtgcgcct caagtatctt accgtgaaac tttccgtgct ggcactcaag
     4261 ccgaaggtaa gtttgtacgt cagtctggtg gtaaaggtca atacggtcac gtatggattg
     4321 aatttacacc aaatgaagaa ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg
     4381 ttgttcctcg tgaatacatt ccagctgttg aaactggttt gaaagatgct atggaaaatg
     4441 gtgtcttagc tggctatcca ttagtagata tcaaagctaa actttacgat ggttcatacc
     4501 atgatgtcga ttcaaatgaa actgccttcc gtgttgctgc ttctatggca ttacgtgcag
     4561 cagctaagaa agctaatcca gtaatccttg aaccaatcat ggctgtagaa gttgttatcc
     4621 ctgaagatta tttaggtgat gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag
     4681 gtatggaagc tcgcgctggc ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa
     4741 tgtttggtta tgctacaact cttcgttctg ctactcaagg tcgtggtaca tttacaatga
     4801 catttgacca ctacgaagat gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg
     4861 gcggaaaagc tgaataattt cttgaaaccg gatagtcctg caaagagatt atccaaagag
     4921 actatatttt taatatagtt tcgtgtaaaa taattatcga tgatatgggc atgtggtgta
     4981 ccttgtgtac atcatatacc tatcagacaa ataaaaaata tattgtattt ttaggaggac
     5041 attttaaaat ggcaaaagaa aaatttgacc gttctaaacc acacgttaac attggtacta
     5101 tcggacacgt tgaccatggt aaaactacat taacagctgc aatcacaact gtactatcta
     5161 agaaaaacgg cggccaagct atggcttacg atcaaatcga tggtgctcca gaagaacgcg
     5221 aacgcggaat cacaatctca acagctcacg ttgagtatga aacagataca cgccactacg
     5281 ctcacgttga ctgcccagga cacgcggatt acgttaaaaa catgatcact ggtgctgctc
     5341 aaatggacgg agctatcttg gtagtttctg ctgctgacgg cccaatgcct caaactcgtg
     5401 aacacatcct attgtctcgt caagttggtg ttccttacat cgttgtattc ttgaacaaag
     5461 tagacatggt tgatgacgaa gaattactag aattagttga aatggaagtt cgtgacctat
     5521 taacagaata cgaattccct ggtgacgatg ttcctgtagt tgctggatca gctttgaaag
     5581 ctctagaagg cgacgcttca tacgaagaaa aaattcttga attaatggct gcagttgacg
     5641 aatacatccc aactccagaa cgtgacaacg acaaaccatt catgatgcca gttgaagacg
     5701 tgttctcaat tactggacgt ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc
     5761 gcgttggtga cgaagttgaa gttgttggta ttgctgaaga aacttcaaaa acaacagtta
     5821 ctggtgttga aatgttccgt aaattgttag actacgctga agctggagac aacattggtg
     5881 ctttactacg tggtgttgca cgtgaagaca tccaacgtgg acaagtttta gctaaaccag
     5941 gtacaatcac acctcataca aaattctctg cagaagtata cgtgttgaca aaagaagaag
     6001 gtggacgtca tactccattc ttcactaact accgtccaca attctacttc cgtacaactg
     6061 acgtaacagg tgttgttgaa ttaccagaag gaactgaaat ggttatgcct ggcgacaacg
     6121 taacaatgga agttgaatta atccacccaa tcgctatcga aaacggtact aaattctcaa
     6181 tccgtgaagg cggacgtaca gttggtgccg gtgtcgttac agaaatcaaa gcttaattca
     6241 cttaagtttt aaagtaaagc aacatttatt cggacgtaat cgctcggaac gaaggtctta
     6301 ggatctttgt tccgagcttt tttatttatt gcaggatcag gaaaaaagac aggagtatta
     6361 taaatagaag aaacacgaaa taaaagagtc agtattttct aaatgaaact ttatgaaaac
     6421 aaaatgtttt ttacaagaaa aagccttgct ttgtgagttt atatatatta taatgacaaa
     6481 ggtgcgttta ctttagtgaa ttgcacagac gtaatcagaa tgatttcgtc ggctaagaaa
     6541 cgagaggttg cgacacgccc ggacgctttg ccacgaacga gcgtgacgga aattttcgtg
     6601 gagctatgtc tacttttcaa aataggcgaa ggagggaaca agatggcaaa acaaaaaatt
     6661 cgtatccgtt taaaagcgta tgaacaccgt attttagatc aatcagcgga taaaatcgtg
     6721 gaaactgcaa aaagaactgg agctagcgtg tctggtccaa ttccattacc aactgaacgc
     6781 agtctttaca caatcatccg tgcgactcat aaatacaaag attcacgcga acaattcgaa
     6841 atgcgtacac acaaacgtct tatcgacatt gtgaacccaa caccaaaaac tgttgatgct
     6901 ttgatgaagc tagacttacc atctggtgta aacatcgaaa tcaaattata aaactaaaac
     6961 aaaaataatg gaggtgtact catgaccaaa ggaatcttag ggaaaaaagt gggaatgaca
     7021 caaatcttca ctgaatctgg tgaattaatt ccagtaactg ttgtagaagc tactccaaac
     7081 gtagtactac aagtgaaaac aatggaaacg gatggctacg aagctattca agttggttac
     7141 caagatatgc gtgaagtttt atcaaacaaa cctgcgaaag gtcatgttgc aaaagcaaac
     7201 acggctccta agcgcttcat tcgtgaattc aaaaatgttg agctaggaga atatgaagta
     7261 ggaaaagaaa ttacagtaga tgttttccaa gcaggagaca ttattgatgt aacaggtact
     7321 acgaaaggta aaggattcca aggggttatc aaacgccacg gacaaagccg cggaccaatg
     7381 gctcacggtt ctcgttacca tcgtcgtcct gggtcaatgg gtccagttgc acctaaccgt
     7441 gtatttaaaa acaaacgtct tgctggccgt atgggtggca accgcgtaac tatccaaaac
     7501 ttggaagttg tacgtgtcga tgcagaaaga aatgttatct tgatcaaagg taatgttcct
     7561 ggagctaaaa aatctttaat tactatcaaa tcagctgtga aagctaaata attgtaggaa
     7621 gaaaggagga actaaggaat gccgaatgta gcattattca aacaagatgg aagccaaaac
     7681 ggtgaaatca cacttaacga agaaatcttc ggaattgaac ctaatgaaag tgttgtttac
     7741 gatgcaatcg taatgcaacg tgcgtcatta agacaaggaa cacacgcggt taaaaaccgt
     7801 agcgctgttc gcggcggcgg acgtaaacca tggcgtcaaa aaggaacagg tcgtgcacgt
     7861 caaggatcta tccgctcacc acaatggcgt ggaggtggcg tagtctttgg accaacacca
     7921 cgttcttaca gctacaaact tcctaaaaaa gttcgtcgtt tagcaatgaa atctgtattg
     7981 tcagaaaaag ttgctgaaaa caacttggta gcaatcgaag gcttgaactt cgacgcacca
     8041 aaaacaaaag aattcaaaca agttcttgct aacttatcta tcgactctaa agtattggtt
     8101 gttctagaaa aaggtaacga ctttgctgct ttatcagcac gtaacttacc aaacgtttct
     8161 gtagtaactt ctgataatgt gagtgtgcta gacgttgtat caaatacaaa agtgttagca
     8221 acacaaactg ctcttactca aattgaggag gtgcttgcat aatgaactta ctagacgtaa
     8281 tcaaacgccc agtgatcact gaaaaatcaa tgcttgccat ggatgacaag aaatacactt
     8341 ttgaagtgga cactcgcgca aacaaaactt tagtaaaaca agcggtagaa gctgcttttg
     8401 acgttaaagt caaaaacgtg aacatcgtga acgtacgtgc gaaattcaaa cgtatgggca
     8461 aatatgcagg atatacaaaa aaacgtcgca aagcgatcgt tacattgact gaagattcaa
     8521 aagaaatcca attattcgaa gctgctgaat aagcactaat agataactca ttaaattagg
     8581 agggaaaaac gtggcgatta aaaagtacaa acctacctca aatggtcgtc gtaacatgac
     8641 aggttctgat tttgctgaaa tcacaacatc aacacctgaa aaatcattgt tacaaccatt
     8701 gaaaaaccat gctggacgta acaacaacgg ccgcatcact gtacgtcatc aaggtggcgg
     8761 acacaaacgc caatatcgcg tgatcgactt caaacgtaac aaagataacg ttgttgcaac
     8821 tgttcaaacg atcgaatatg atccaaaccg ttcagctaac atcgcgttag tacactatga
     8881 agatggagtg aaagcttaca tcttagcacc taaaggacta gaagtgggta tgaaacttgt
     8941 ttcaggtcca gaagcagata tcaaagtcgg aaatgctctt ccattagaaa acattccagt
     9001 tggtactgtt atccacaaca ttgaaatgaa acctggtaaa ggtggacaat tgatccgttc
     9061 tgctggtaca agtgctcaag tacttggtaa agaaggcaaa tacgtattga tccgcttgaa
     9121 ctctggtgaa gttcgtatga tcttagctgc atgccgtgct acaatcggtt ctgtaggtaa
     9181 cgaacaacac gaattaatca acatcggtaa agctggacgc tctcgttgga tgcgtaaacg
     9241 cccaactgta cgtggtagcg taatgaaccc taatgatcac ccacacggtg gtggtgaagg
     9301 taaagctcct atcggacgta aagcaccagt ttctccttgg ggtcaaccag ctcttggtta
     9361 caaaactcgt aacaaaaaag ctaaatcaga caaacttatt gttcgtcgtc gcaaaaataa
     9421 ataattactt tgaccatgtg tcgcacattt tgagaggagg ttcaccatgg gtcgtagttt
     9481 aaagaaagga ccttttgtcg atgagcattt aatgaaaaaa gttgaagctc aagcaggggt
     9541 tgaaaagaaa aaagttatca aaacttggtc acgccgttca acagttttcc caagttttgt
     9601 tggatatacc atcgcagttt atgatggacg taaacatgta ccagtttaca tccaagaaga
     9661 catggtagga cacaaattag gtgaatttgc acctacaaga acttatcgtg gacatgctgc
     9721 tgacgataag aaaacgaaac gctaatttga ggggaggata aatccaaatg gcagaacaaa
     9781 tcacatcagc taaagcaact gcaaaaacag ttcgcacttc acctcgaaaa gcccgtttag
     9841 taattgacct tatcagaggt aaaagtgtcg cagatgcaat ttcaatcttg aaattcacac
     9901 caaacaaatc tgctggaatc attgaaaaag tattgatgtc agcagttgcg aatgcagaaa
     9961 ataactttga cttagatgtt gaaaacttgg tagtaagcga agcttttgtt aacgaaggac
    10021 ctacaatgaa acgtttccgt cctcgtgcaa aaggatcagc ttctccaatc aacaaacgta
    10081 caagtcatat cacggtagtc gtatcagaaa aataaggagg gataatcagt gggtcaaaaa
    10141 gtacatccaa ttggaatgcg tgtaggcatc atccgcgact gggatgctaa atggtatgct
    10201 gaaaaagagt atgctgagtt cttacacgaa gatttaagaa tccgtaaatt tatttcaact
    10261 aaacttgctg atgctgctgt atcaacagtt gaaatcgaac gcgcaacagg tcgcgtaaac
    10321 atttcaattc acacagctaa accaggtatg gttattggta aaggcggttc tgaagtcgaa
    10381 agcctaagaa aagaattaaa caaattaact ggtaaaagaa tccatatcaa tatcgtggaa
    10441 atcaaaaaac cagatctaga tgcaaaatta gtaggtgaag gaatcgcacg tcaattagaa
    10501 aaccgtgttg ctttccgtcg cgctcaaaaa caagccatcc aacgctcaat gcgtgctggc
    10561 gctcaaggga tcaaaactca agtatctggt cgtctaaacg gagcagatat cgctcgttca
    10621 gaaggatact cagaaggaac tgttcctctt catactttac gtgcggacat tgattacgca
    10681 tgggaagaag cagatacaac atacggaaaa ctaggagtta aagtgtggat ctatcgtgga
    10741 gaaattcttc caactaaaaa aaacactgag aaaggaggga aataatcatg ttagtaccta
    10801 aacgtgtaaa acaccgtcgt gaattccgcg gaaaaatgcg cggggaagct aaaggcggaa
    10861 aagaagtagc attcggtgaa tacggtttgc aagctgttga ttcacattgg atcacaaacc
    10921 gccaaatcga agctgctcgt atcgcaatga ctcgttacat gaaacgtggt gggaaagtat
    10981 ggattaaaat tttccctcac aaatcttata ctgccaaagc aattggggta cgtatgggtt
    11041 ctggtaaagg ggcacctgaa ggatgggttg caccagtaaa acgtggtaaa atcatgtttg
    11101 aaatcgcagg cgttcctgaa gaagtagctc gtgaagcgtt acgtctagct tctcacaaat
    11161 taccaatgaa aactaagatc gtaaaacgtg aggaaatggg tggtgaatcg aatgaaggtt
    11221 aaagaaatca gagaattaac cactgccgaa atgcttgatc aagaaaaaca attaaaagaa
    11281 gaattgttta atcttagatt ccaattagca acaggtcaat tagaaaacac tgcacgtatt
    11341 aaagaagtac gtaaatcgat tgcacgcatc aaaactgtgt tgcgtgaaca agctaagtaa
    11401 ttatggaagg aggccattaa gcgtatgact gaaggaagaa atcaacgcaa agtttatcaa
    11461 ggtcgcgtgg tttcagacaa aatggataaa acaattaccg ttgctgtcga aacaaagaaa
    11521 aaccacccta tctatggcaa acgcatgaac tattctaaga aatacaaagt gcatgatgaa
    11581 aacaacacag caaaagttgg cgacatcgtg agaattatgg aaactcgtcc attatcagct
    11641 acaaaacgtt tccgtttgtt ggaagtagtc gaagaagcag ttattatcta ataacacgag
    11701 attttcctat atagattgaa tgcaaaatct gaaaggagga tacacaacgt gatccaagca
    11761 gaaagtcgtt taaaagtcgc tgataactca ggcgcacgtg aaattttgac gatcaaagtc
    11821 cttggtggat ctggtcgtaa aactgctaac atcggtgatg tgatcgttgc tacggttaaa
    11881 caagcaacgc caggtggggt tgtcaaaaaa ggtgacgtcg ttaaagccgt tatcgttcga
    11941 acaaaatcag gagctcgtcg tacagacggt tcatatatta aatttgatga aaatgctgca
    12001 gtgattattc gtgatgataa aagccctcgt ggaacacgta tctttgggcc agttgcacgt
    12061 gaactacgtg aaaacaactt catgaagatc gtttcactag caccagaagt tttataatct
    12121 tgagacactt atcaaaggag gtgcgaaaca gttatgtttg ttaaaaaagg cgataaagtt
    12181 aaagttatca ctggaaaaga caaaaacaaa gaaggcgttg tactagcagc gttcccgaaa
    12241 aaagataaag ttattgtcga aggtgtaaat gtcgttaaaa aacaccaaaa accttctcaa
    12301 gctgctcctc aaggtggaat tgttgagatg gaagcgccaa ttcatgtttc taacgtgatg
    12361 gtggttgact ctactggtgt agctggccga gttggctaca aagaagtaga cggcaaaaaa
    12421 gtccgtgttt ctaaaaaaac cggtgaagtt ttagataaat aaaagtttaa ggaaggaggg
    12481 tcttactgaa tgaaccgcct aaaagaaaaa tatcttaaag aagtaactcc atcattgatg
    12541 gaaaaattta actatagctc tgttatgcaa acacctaaag ttgaaaaaat cgtcatcaac
    12601 atgggtgttg gtgatgcggt atcaaacgct aaaaacttag ataaagcagt tgaagaatta
    12661 actttgatca ctggtcaaaa accaatgatt acaaaagcta aaaaatcaat cgctggattc
    12721 cgtttacgtg aaggaatgcc aattggtgca aaagttacat tgcgcggaga aagaatgtac
    12781 gaattcttag acaaattggt ttctgtttca ctaccacgtg tacgtgactt ccatggtgta
    12841 agtaaaaaag cctttgacgg tcgtggaaac tatactttag gtattaaaga acaattgatc
    12901 ttcccagaag tagattatga tttagtagat aaagtacgtg gtatggacat cgttattgta
    12961 acaacagcaa actctgatga agaatctcgt gagttgttga cacaattagg catgccattc
    13021 caaaaataat taaaggaggc gaactacgtg gctaaaaaat caatgattgc taaaaacaaa
    13081 cgtcctgcaa aattttcaac acaagcttat actcgttgcg aacgttgcgg acgtccacat
    13141 tcagtttatc gtaagttcca tctttgccgt atttgcttcc gcgaacttgc ctataaaggt
    13201 caaattcccg gcgtgaagaa agctagctgg taaaaagtaa attcgcataa gaaggaggta
    13261 aaagatcaat ggtcatgaca gatccaattg cagattttct aactcgtatc cgtaacgcaa
    13321 acatggttaa acatgagtct ttagaagtac ctgcatcaaa aattaaacgt gacatcgctg
    13381 aaatcttgaa acgtgaaggt ttcgttcgtg atgttgaata catcgaagat gacaaacaag
    13441 gcgtgatccg tgttttccta aaatttggta aaaacggcga acgtgttatc acaaacttaa
    13501 aacgtatctc taaaccaggt ttgcgtgctt atgtcaaagc tgacgaagta ccaaaagtat
    13561 tgaacggttt aggtatcgca atcatttcga cttctgaagg tgttgtcact gataaagaag
    13621 ctcgtgaaaa aaatatcggc ggcgaagtta tcgcttacgt atggtaatca agtagaatct
    13681 acaaggaggt gtctttaagt gagccgtatc ggaaataaag tcgttgttat ccctgaagga
    13741 gtaacagtta ctcaagacgg aaacaacgtt acagttaaag gaccaaaagg tgaattgact
    13801 cgtacttttt cagcagatat caaaatgaat atcgaaggaa acgaagtaac tttcactcgt
    13861 ccaaacgaca gtaaagaaat gaaaacaatt catggaacaa ctcgtgcgaa cttcaacaac
    13921 atggttgtag gtgtcagcga aggtttccaa aaagcgttag aactaatcgg ggttgggtac
    13981 cgtgcacaat tacaaggatc aaaacttgta ttgaacgttg gttactcaca tccagtagaa
    14041 atcacaccac cagctggagt aactgttgaa gtaccagcta acacacaagt aattgttaaa
    14101 ggtgcgaaca aagaagaagt tggcgaatta gctgcaaaca ttcgtggaac tcgtcctcca
    14161 gaaccttata aaggtaaagg tattcgttat gttggcgaat atgtacgccg taaagaaggt
    14221 aaaactggta aataatcgtt tagaaaaatt gcatagaagc cagcagcagg aaaaaggcat
    14281 ttggctgaag tttaccttca aaaaaataca tttttacttg tgccgcagct tctatttttc
    14341 gaaacgtatt tactgcttga aatagtagta caataaaaat aaagaggtga caattgtgat
    14401 tacaaaacca gataagaaca aaacacgtca aaagagacat cgtcgtgtac gtaacaatat
    14461 ctctggtact gctgagcgcc cacgcttgaa cgtttttcgt tctaacaaaa acatctacgc
    14521 tcaaattatt gatgacgtag cgggtgtaac gctagcaagt gcctctacct tggataaaga
    14581 aatttcaggt ggaacaaaaa cagaagcagc acaagctgtc ggtaaactag ttgctgaacg
    14641 tgcagctgaa aaaggcatta aagtagtagt ctttgaccgt ggtggatacc tttaccatgg
    14701 ccgtgtgcaa gctttagctg aagctgctcg cgaaaatgga ctagaatttt agaagaagga
    14761 ggaataccat tcatggttta tatcgatcca aaacatttgg aattagaaga ccgcgttgtt
    14821 gcaattaacc gtgtaacaaa agttgttaaa ggtggacgtc gtctacgttt tgctgcttta
    14881 gttgttgtcg gtgacaaaaa cggacacgta ggatttggta caggtaaagc tcaagaagtt
    14941 cctgaagcaa tccgtaaagc aattgaagat gcgaagaaaa acttagttga agtacctatg
    15001 gttggttcaa caatcccaca cgaagtaatc ggtgctttta gtggtggtag aatcctaatg
    15061 aaacctgctg tagaaggttc tggggttgct gctggtggac cagttcgtgc cgtattagaa
    15121 ttagctgggg tagcagatat cacttctaaa tctttaggct caaatacacc aatcaacgtt
    15181 gttcgcgcaa cagttgaagg tttgaaacaa ttgaaacgtg cagaagaagt tgcagaactt
    15241 cgcggcaaat cagttgaaga attaatcgga taaggaggac aacaataatg gctgaattaa
    15301 aagttacttt aaaacgcagt attatcggac gtcctcaaaa ccaacgcgat acagttaaag
    15361 cgttaggtct tacaaagatt aacagttcag tggttaagcc ttctaatgaa gcaattaaag
    15421 gcatgatcaa tactgtttct cacctagtgg acgttgaaga aatttaattt agaaaaatca
    15481 taactagaac attaaggagg tgccaaacct atgaaacttc atgaattaaa acctgctgaa
    15541 ggttcacgcc aagtacgtaa ccgtgttggt cgtggtactt catctggtaa cggaaaaact
    15601 gccggacgcg gacaaaaagg acaaaaagct cgttcaggcg gcggtgtgcg tttaggattt
    15661 gaagggggac aaacaccatt gttccgtcgt ttaccaaaac gaggattcac aaacgtgaac
    15721 cgtaaagact atgcagtcat taatcttgat gtattgaacc gctttgaaga cggagctgaa
    15781 gtaactcctg ttgctttagt tgaagctgga atcgtgaaaa acgaaaaagc tggaatcaaa
    15841 gtattagcta atggggaact aaacaagaaa ttaactgtga aagcagcaaa attctctaaa
    15901 gcagctcaag aagcaattga agcggctggt ggatcaatcg aggtgatcta atgtttagac
    15961 tattaaaaga tgcttttaaa gtaaaagaca ttagatcgaa aatcctattt actgtatttg
    16021 tgttgtttgt atttcgccta ggtgcacata ttactgtacc aggcgtaaat gctgcttcat
    16081 tgcaaaacct ttcttctttg ccatttttca acatgttgaa cttggtaagt ggtagtgcga
    16141 tgcaaaactt ttccattttc tcaatggggg tttcaccata cattactgct tctatcgtca
    16201 ttcagcttct tcaaatggac atcgtgcctc gatttgttga atggtcgaaa caaggggaag
    16261 ttggtcgtaa aaaactgaat caagcgactc gatatctgac attagtattg gcatttatcc
    16321 aatctgtcgg tatcactgct ggattcaatc aatacgctca attaggtttc gtgaataatc
    16381 ctaacgtagc aacttacgta acgatcggtt tgattctaac agccggtacg atgtttgtta
    16441 cttggctagg agaacagatc acagaaaaag gtatcggaaa tggcgtatca atgatcattt
    16501 ttgcaggtat catttctaga ttgcctgaaa gtatcagaga aatttatgaa gattacttta
    16561 taaatattga tccttcagac atatggaaat ctgctatttt tgtggccatt ttactagtag
    16621 cggtcttgtt gatcgtgaca ttcgtaacat ttttccaaca agctgaacgc aagatcccga
    16681 tccaatatac gaaacgcgta gctggcgcgc ctacaagcag ctatctgccg ttgaaagtaa
    16741 atgcagctgg agttattcca gttatctttg ctagttcgtt tattgctacg ccaaatgctg
    16801 ttttacaagc attgagcggt agattcggtt ctgaaaactg gtacaaagtg atgatggaga
    16861 tctttaacta taatacagca cctggtgcta cgatttatac gattctgatc gttgccttca
    16921 ctttcttcta cgcctttgtt caagtaaacc ctgagaaatt agcggaaaac ttgcaaaagc
    16981 aaggaagtta tataccaagc gtacgccctg gtaaggggac tgaagagtat gtctcaagat
    17041 tactgatgcg tttaagtaca gttggtgcag tcttccttgg tttagtggca ttgcttccaa
    17101 tcattgctca aatggtttgg gatcttccac aatcaatcgg attaggcggt acaagcttac
    17161 tgatcgttat cggtgtagct ttagaaactg cgaaacagtt agaaggctta atgctgaaac
    17221 gtaaatatgt tggattcatt gagtaagaaa gcgtgttgag gtttcccctc aacacacgtt
    17281 ctcacacatt tattaggagg gaaaacaatg aacctcattt taatggggct gccaggcgca
    17341 ggtaaaggaa cacaagctga aaaaatcatt gatgcttatg gtattccgca catctcaaca
    17401 ggagatatgt tccgcgcagc gatgcaaaac gaaacagctc ttggcctgga agcaaagtct
    17461 tatatggata aaggtgcatt agttcctgat gaagtaacaa atggaatcgt gaaagagcga
    17521 ctagctgaac cagatacaga gaaaggcttc ttattagatg gctttccacg tacgctagat
    17581 caagcaaaag ctctggacgc aatgctaaaa gatttgaata aaaaaattga tgctgtcatc
    17641 gatatccatg ttggtgaaga aatcttggtc gaacgtctag ccggccgatt tatctgcagc
    17701 aattgtggag caacttacca taaaattttc aaccctacaa aagttgaaga cacatgtgat
    17761 cgttgcggtg ggcatgaatt ctatcaaaga gaagatgata aacctgagac ggttaaaaat
    17821 cgtctggcta tcaacatcaa aaatagtgaa ccaatcttgg cttattataa agaacaaggt
    17881 ttgctaaaca ctattgatgg tgctcgtgaa atcgatgctg tatttgcaga tgtacaaaaa
    17941 atcattgaaa aatagcacga ttgcccgtag ccgacttgca ttatgcagtt actcttgtca
    18001 agttcaataa attatgatag aatatgtcag tccgacttct agagaatcct ctagatgatc
    18061 cattttgaga attttgaggt gggaactgca gtttccgcca ttttatcgtg tgaaaatgcg
    18121 aaacaagtac aaggaggtac tgtgcgtggc aaaagaagat atgattgaag tcgaaggtac
    18181 agtcgtcgaa actttgccga atgcaatgtt taaagttgaa ctagaaaacg gacaccaagt
    18241 tcttgctact gtttcaggta aaattcgtat gcattacatc cgtatcttac ctggtgacaa
    18301 agtaacggtt gaattgtctc catacgatct taaccgtggc cgtattactt atcgctttaa
    18361 ataattgtac tccgtaaaac tacaggaggt attatcatga aagtaagacc atcagtaaaa
    18421 ccaatgtgcg aacattgtaa agtaattcgt cgtaaaggac gcgttatggt gatttgccca
    18481 gcaaatccaa aacataaaca acgtcaagga taattggagg tgctataaat atggctcgta
    18541 ttgcaggagt agatattcct cgtgataaac gtgtagtagt ttcacttact tatatctatg
    18601 gtattggtaa cactactgct aaaaagatct tagctgatgc tggtgtatct gaagatgttc
    18661 gcgttcgtga attaacgaat gaacaaacag atgctatccg tgcagaagta gataaattaa
    18721 aagttgaagg ggatcttcgt cgtgaagtga acttgaacat caaacgtttg atggaaatcg
    18781 gttcttaccg tggaatccgt catcgccgtg gattgcctgt tcgtggacaa aacacgaaaa
    18841 acaatgcacg tactcgtaaa ggcccagctc gcgctatcgc tggcaagaaa aaataattct
    18901 taagtgaagg aggttaaaac ttcatggcag caaaaaaagt gaatcgtaaa cgccgtgtga
    18961 agaagaatat agaagcaggt attgcacata tccattctac attcaataac actatcatca
    19021 tgatcactga tactcacgga aatgcattag cgtggtcatc agctggttca ttaggtttca
    19081 aaggaagcaa aaaatcaact ccttttgccg ctcaaatggc agcagaaact gctacaaaag
    19141 cagcaatgga acacggatta aagactgttg aagtaacagt taaaggacct ggttctggac
    19201 gtgaagcagc aattcgttca ttacaagcta caggtttaga agtgactgca attcgtgacg
    19261 tgactccagt tcctcataat ggtgcccgcc ctccaaaacg ccgtcgtgtt taatgagtgc
    19321 cgctcatttt gagttttatc agtaacgtca ttgaacaaga cacttttcgt tttgaaaggg
    19381 gtaaagataa gaatgattga gttcgaaaaa ccaagaatcg caaaaattga tgaagaaaaa
    19441 gattatggca agttcatcgt tgaacctctt gaaagagggt atgggactac tttagggaat
    19501 tccctacgtc gtattttatt atcttctttg ccaggtgctg ccatcactaa tattcaaatc
    19561 gatggtgtac tgcatgaatt ctctaccatc aaaggtgtta gagaagacgt cactcaaatc
    19621 atcttgaaca ttaaaggctt agcgctgaaa atgtatggcc aagaagaaaa aacccttgaa
    19681 atcgatatta ccggacctgc tacagtaact gctggcgata ttatcgttga tagtgatgta
    19741 gaaattctta acaaagatat gtacatctgt agtgtctctg aaggagctac gttccatgct
    19801 cgcttaacag tgaaacctgg acgtggttat gttcaagcag atgaaaataa aaaagaagat
    19861 atgccaattg gcgttcttcc agtcgattca atctacacac ctgttcgtcg tgtgaactat
    19921 caagttgaga acacacgtgt tggacaccgt gaagattttg ataaattgac gatggagatt
    19981 tggacagatg gttcaattga accgttggaa gcaatgagtt tgtctgcgaa aatcatgact
    20041 gaacatttgg atatttttgt gaacttaact gatgaagcaa aaaatgctga aatcatggtc
    20101 gaaaaagaag aaacacaaaa agaaaaaatg ttggaaatga cgatcgaaga gttagacttg
    20161 tctgttcgct cttacaattg tttgaaacgt gcaggaatca atactgtaca agaacttaca
    20221 aataaatctg agcctgaaat gatcaaagtc cgtaacttag gacgtaaatc attagaagaa
    20281 gttaaagcaa aattgcacga cttaggttta ggtttacgta aagaagatta aaacctttta
    20341 cgaaggaggg aaaccaacgt gagttatcgt aaactaggac gcacatctag ccaacgtaaa
    20401 gcgatgttgc gtgatttaac aactgattta ttaatcaacg aacgtattgt gactactgaa
    20461 gctcgtgcga aagaagttcg ctcaactgca gaaaaaatga tcactttagg taaacgcgga
    20521 gatttgcatg cacgtcgtca agctgcggct ttcgtacgta atgaagttgc cagcgtgcgt
    20581 gaagaaaatg aggacatcgt cattgaatca gctttacaaa aactgttcaa cgatattgca
    20641 cctcgttacg cagaacgtca aggcggatac acacgcattt tgaagacaga accaagacgc
    20701 ggtgatggtg caccaatggt tgtcttagaa cttgtctaaa atcacactaa taacatcact
    20761 ttatagatga ttcttttaga gtgttatgat gttggaagat atttttccga gtctagctca
    20821 gcgctgctcc tcaagcgaat atcgcttggg gagtaagcac tcatcataaa gtgttttttt
    20881 ataccttcaa ttacctgtca tgacagatat gtttgaaggc ataaaaaaac attttaagcg
    20941 agaaataatt gaaaattttg tctttttttg atagtgttag taggaacttt aggatgaaga
    21001 aggagtaatc atggagccaa ttattgaatt agaaaagata aattataaat accagccaga
    21061 tgatcttcgc cccgcattga aagatgtctc ctttacaatt gataaaggtg agtggatcgc
    21121 tattattgga cacaatggtt caggaaaatc gacacttgcc aaaacgatca atggattgct
    21181 tctgccagaa tcagggatcg tgaaagtggg aaatcaaata ttggatgaag aaaatatttg
    21241 gacgatccga cagatggttg gaatggtttt tcaaaatcca gacaatcagt ttgttggttc
    21301 gacagtagaa gatgacgttg cttttggtct tgaaaatcaa gggattccca gagaagaaat
    21361 gctagtgcgt gtgaaagatg cacttgagaa agttagaatg gctgagtttg cttcacgcga
    21421 gcctgctcgt ttatcaggag ggcaaaaaca acgtgttgcc attgccggcg tggttgcctt
    21481 acgacctgat atcattattt tagatgaagc aaccagtatg ctagatccag aaggtcgtga
    21541 agaagtgatc tctacgatca agaagatcaa agaagaaagt caattgaccg ttatatctat
    21601 cactcatgat attgatgaag cagctaatgc caatcggata ctagtgatga gacaaggcga
    21661 acttgtccgt gaaggtacac caaaagaaat tttttctgct ggtcctgaac tgatcgactt
    21721 aggtctagat ctaccatttc ctgaaaaact gaagagcgca ctgaaagaac gtggggttga
    21781 tgtgcccagt gaatatatga cagaagaaag gatggtggat tggttatgga catccgtttt
    21841 gaacaagtag acttcactta tcagccgaat acaccatttg aacaaagagc cttatttgac
    21901 atcaatatga cgatcaaaga aaacagttat actgcattag tagggcatac cggaagcggg
    21961 aaatcaacct tacttcagca tttgaatgcg cttgttaagc caacaagcgg tacagtccat
    22021 attggagaac gagatattca gccggatacg gataataaga atttaaagcc catccgaaaa
    22081 aaagttggca tcgtctttca gtttccagaa gcacagctat tcgaagaaac ggtagcaaaa
    22141 gacattgctt tcggtcctaa aaactttggt gtcagcgaag aagaagcact agtcctagca
    22201 aaagaaacat tagaacaagt tgggctggat gaaagctatt tggaacgttc gccatttgaa
    22261 ctttcaggag ggcaaatgcg tagagtagca atcgctggtg tgcttgccat gaggccggaa
    22321 gtacttgtat tggatgaacc aacggcagga cttgatcccc agggcagaaa agaaatgatg
    22381 gagatgtttt ggcgtcttca taaggaacag cagatcacta ttgtattagt tacgcattta
    22441 atggatgatg tagccaattt tgcagattat gtctatgtgt tggaaaaggg acgaatcgtt
    22501 aatagcgggg agccgcaaga ggtctttcaa aacattgaat ggctaaaaga aaaacaacta
    22561 ggcgtaccta ctgccaccga gtttgcagaa gaattaatgg ctaaagggat gaactttgct
    22621 accttgcctt tgacagctga agaattagca gacgctatcg tccattatgc tggaggaaat
    22681 gcccatgatg aataaattga tatttggtcg ttatatgcct ggagattcct tgatccatcg
    22741 attggatccc cgagctaaat tattagccag tttttatttt atcggaatca tttttttagc
    22801 aaataattgg gaaacgtacc ttttgttggc atttttcaca ttactttctg tcttcttgtc
    22861 aaaagtagat ttgaaatttt ttatccgagg cattcgccca ttgatctggc taatcctctt
    22921 cactgtagct ttgcaggtct tgtttacaag tggcggagaa acttattgga gttggggtgt
    22981 tttccatatc actgaatttg gtgtgatgaa cggcctcttt attttctgtc gattcgtgtt
    23041 gatcattttt atgtcgactc tactcacgct tactacaccg cctttggaat tatcggatgc
    23101 gattgagtac ttgcttcgtc cattgaaagc cgtacggttc ccagtccacg aaatttcatt
    23161 gatgctgtcg attgctcttc gttttgtgcc tacattgatg gacgaaacag aaaagatcat
    23221 gaacgctcag cgcgctcgcg gtgttgattt tggtgaagga aacctagttc agaaaatgaa
    23281 agcagtcatt cctttgctga ttcctttatt tgtcagcagc ttcaaccgtg cggaagatct
    23341 tgcgactgct atggaagcaa gaggctacaa aggtggggaa ggaagaacga aatatcgtgt
    23401 attgcattgg cataatagag acacgatggt tatcattgct tttgtattat taactgtgct
    23461 actgatcttt ttaagaggat aaacgttcag tgggccgcta tctattcttt tcttgctgat
    23521 ctgtttgcaa gaaaggatgc aagcagtgta tgacaagaaa tattagggtc tgtggcagac
    23581 taaggtcaca ggcctctttc ttttatctga aaagaaagaa cgaaaaaatt ttatagaaaa
    23641 atcattattc cagataacct tttaggaaca aaggagagaa aaacatggta cgttacaaag
    23701 caatcattgc gtacgacgga acaaatttta atggattcca aaaacagcca aatgggcgta
    23761 ctgttcaaga agaagtagaa aaaacattac aaaaaatggc aaatggaaaa gaaatcacgg
    23821 tctttggttc gggaaggaca gatgccggag tacatgcgat cgggcaagta atccattttg
    23881 attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc cagtcaccag
    23941 aggatattgc tgtaagacaa gtagaaattg tttcagaaga cttccacgca agatatttgg
    24001 tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa accacgcagt ccttttcgac
    24061 gccattatgc aagttatttt ccttacccac ttgatttgga aaaaattcag cgggcgcttc
    24121 ctgatttatt agggactcac gatttcacct ctttttgtgc atcaggaagc tcgatagagg
    24181 ataaaatacg tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa gacgaattgt
    24241 tgtttacatt taggggaaat ggcttcttgt ataagatgat acggatctta gtaggaacgc
    24301 ttttgaaaat tggcaatggc cgattgcctg aagatagtat cccagatatt attgcgaaaa
    24361 aagatcgtaa tgcagcagga cctacagcac accctgaagg actttatttg tatgaagtga
    24421 aatatgaata agaggttatg atagaagtgg tcagcttcaa gaaataagaa ggtatttacg
    24481 aaaattactt ctcaaaattt tgcaaaattt cggcttattt tcgaagaagc tacttctgga
    24541 acaccgttta aggaagaggt cgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
    24601 cgggaaaata gctcctcata ttttgccgtt tgttgagcta ataccgaagg tcagctgatt
    24661 gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca caaagagcaa
    24721 tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga ggcgttgata
    24781 agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg aacgttgttt
    24841 tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt ttctacgtct
    24901 tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc cataaaaggg
    24961 gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata ggacaaaaag
    25021 gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat gtttacctgc
    25081 tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg gtttcccatc
    25141 aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg aatcttcagc
    25201 ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac ctttttcttt
    25261 gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt taggctccat
    25321 gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg tctcttgagg
    25381 ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc gcgatgtacc
    25441 attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg ggaacaaact
    25501 taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt ttggctggtt
    25561 atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg tattcaactg
    25621 attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag acaatgtatt
    25681 gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat cattaacgga
    25741 caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg tatagtcgcc
    25801 gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt tagaaatgtt
    25861 atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac ggatattcaa
    25921 atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat tacgatttct
    25981 tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt ttgcagatac
    26041 tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt catttacaag
    26101 agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat tccaatcatc
    26161 aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca tgatcaataa
    26221 taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc ccacagcaaa
    26281 agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca cataggtgta
    26341 gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga ccaaaatgat
    26401 tcctaacaac agcccacgat cgtcttgttc tatcttttcc catccttttc ctgcaagcag
    26461 gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg gtgcatgcat
    26521 accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta tggcgaaaag
    26581 actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat aaaacagaca
    26641 gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt ttgtcgtgtc
    26701 ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt ctgttttcaa
    26761 cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg ctggcaatac
    26821 aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa caatcgtttt
    26881 tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag aaaatacgcc
    26941 aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga taaataaaag
    27001 tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg gaagataaaa
    27061 aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga tcaatgcgta
    27121 actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg caagaaacca
    27181 aaaagccaat ccggcacagc cgaccttaag caaagtcaga acatacagtg catctggcat
    27241 catctggttt ggaaaaaaga acaccagtgg cgtgaagatc ccgcctagat agtaagaaat
    27301 taaagataaa taattcaatc ctagagaagc attccatgta taaaaaatac tttgctttcc
    27361 aagaagaaca tttcggaagc tggcatgaaa attagaaaac tgtgaaaagg catcacttgc
    27421 caaaatactt ctgctgcttc caggatagat tccgatactt agataaacaa gtgctaaaat
    27481 caaaaacgga atcaggaaac tagctgctgc gtagcgtccg ttttctctaa agaatgactt
    27541 taaatttttt ttcatattta ttttccacca aatttctatg aagttgtatt cagtttatca
    27601 tatccttgtt tttctctgct taaaaagagt ctagttttca caaaaaaata aaatttagaa
    27661 taaataactt cataatttgt tcgtataaca aaaataaacg caaaaaaagg gtaggaatca
    27721 aaagtcccac ccttaaaaat ttttattaaa taaaaaaccg ttcactatct gaacggtcca
    27781 agtatcattc aattaaacgc gttcaacttt tcctgatttc aaagcacgag ttgacaccca
    27841 aactttttta ggttttccgt cgaccataac gcgaactttt tgtaagttag gtttaacagt
    27901 acgttttgtt gagttcatag cgtgtgaacg gttgtttcca ctttttgtct tgcgacctgt
    27961 aaagtaacaa acttttgcca tggtaatttc ctcctttgcc ttgatttgga gcaatagaat
    28021 aattcagctc ataccagact aacatatcat atagaatggg aatttgcaag tacattttca
    28081 tagatacaat tgacaattga gaagaataat tgtaaattaa acaaggttat acctgaaatt
    28141 ttgcaaggtt tttgagtact ttgggtaact gccttttatt tcaagttgcg ctatgataaa
    28201 atatgataga agaaaaatgg gagtttttta cccgttaagg aggctcattc atggctgtaa
    28261 aaattcaaac gtcagctgga accattgaaa ttaccaatga tgtaattgca acagttgttg
    28321 gtggcgccgc gacagatgtg ttcggtgttg taggaatggc cagcaaaaat caaattaaag
    28381 ataatattac tgagattttg cgtaaagaaa attactcaag aggagtagtt gtccgccaag
    28441 aagataatgg tattgctgta gatgtttata cgattatcag ctatggtacg aaaatctctg
    28501 aagtttcccg caacgtccaa gaaaaagtga aatacaatct agaggcatta ttaggcgtaa
    28561 ctgccaattc agttaatgtc tttgtacaag gtgttcgtgt actgccagac taggcaggag
    28621 actggctggt aactcagcag tcgatctgaa ggaggattat ctaggtgagt gtaacagaaa
    28681 taagtgcaag ccagttccag gaaatggtcc aggcaggtgc gaatcgtctg caagtaaatg
    28741 cagaatacgt caattcatta aatgtattcc ctgtgccaga tggcgatacg ggaacaaata
    28801 tgaatctatc tatgactagt ggagctaagg cagtagtaga ttcaacctca gaaaaagtag
    28861 gagaattagc agcaatgctt tcaaaaggat tgctgatggg tgcgcgcgga aattccggcg
    28921 ttattttgtc tcaattgttt cgtggatttt ctaagcaaat tccagatgtt gtctcattaa
    28981 atgcttctga tcttgctgca gccttcactc atggagttga aactgcgtat aaagcagtaa
    29041 tgaaaccagt agaaggaacg attttgacag tagctagaga agcagcgaaa gctggagaaa
    29101 agaaagcaaa aagtactgat gatgtcgttg aagtaatgac agctgttgta aaaggagcaa
    29161 agcgtgcttt agctaaaact ccagacttgc ttccagtcct aaaagaagtc ggtgtcgtag
    29221 acagtggagg gcaaggactg ctgtttgttt atgagggctt tttaagtgca ttgaatggag
    29281 aattccaagc ggatgaaaca tatgaacctt ctccagcaga aatggatgat atggtcaatg
    29341 cagaacatca tcgcagtgtc caaggtcaaa tggcgacaga agatatccat tttggttact
    29401 gtacagaaat catggtaaaa atcggagaag gacctacagt cgatagtaca ttcgattacg
    29461 aagaattccg taactattta gatggtatag gagattcttt acttgtggtc aatgatgacg
    29521 agatcatcaa agtccacgtg catacagaaa acccaggaga agtcttgaat tatggacaaa
    29581 aattcggttc attagtcaaa atcaaagtcg acaacatgcg cttgcagcat gagacgattt
    29641 tggaacacga tgaagaagtg aacgagtttg cgaaaaaaga aataccacgt atcccgtatg
    29701 gtatcgtagc gatcgctgct ggtaaaggcg tccaagagct gtttgaaagt ttaggcgcta
    29761 attatgttat cagtggtgga caaacaatga atccaagtac ggaagacatt gttaaagcga
    29821 ttcaagaagt aaacgcagat aaagtgattg tcttgccaaa caataagaac atcttcatgg
    29881 cagctgatca agcagcagaa gtagcagata tcccagtagc agttgttcct tcaagaacga
    29941 tttcccaagg attgacagct atgctttcat tcaatgatca agcttcttta gaagaaaaca
    30001 aagaaacgat gacagaaatg ttgtctagcg ttgtgagcgg tcaagtcact cacgcgatca
    30061 gagatacctc aattgacggt gtagagatca aagaaggaga ctttttgggc atgatcgatg
    30121 gaaaaatcct gatctctaac ccagatatca cagcaacagc gttaagtaca ctagaacaaa
    30181 tgatcaatga ggacacagag atcgtaacga tcttggcagg tgatgaagga acagttgaac
    30241 aagcaaatga atttgctgag aaactaacag aaaaacatcc tgatttggaa atcgagatcc
    30301 accaaggaga tcagcctgtc tatccttatc tattttctgc tgaataattt ctgtgtcaga
    30361 atatctttct aacacagtat tcattcataa aaggccttcg gttagtgatc aggtatgatc
    30421 gctgaccaag tccttttttt gttaaaggag ggaggaaaaa tttatgcgtt ctttagatga
    30481 tccaatcact atgctggcag gagtcggaga aaaaagagca gctagtcttg ccagcttggg
    30541 catccaaacg attgaagact tactgactta ttatcctttt cgctacgaag atattcagga
    30601 aagaaatcta aacgagatcc aggatcaaga aaaggttact ttgaaaggaa tcgtagtctc
    30661 gcctcctgtg atgaatcgat ttggttataa aaaaagccga ttgcaatttc gtatgatgca
    30721 agaacatgat gtgttcaacg tttctttttt taatcaacct tatctaaaag aaaaagtcat
    30781 tttatcggag gaaatcgccg tatacggtaa atgggatgca aagcgtaaag ccttgaacgg
    30841 gatgaagatt ttaggttctc aaacaatgga tgacttttcg ccgatatatc acgtcaacaa
    30901 atcaattcgt caaacgacat tagttgatct aatcaaaaaa gggtttcaag aatacggcac
    30961 attgattgaa gaaaatctgc caaacaacct tgtggagaaa tatcgactgc ttgaccgagc
    31021 ttctgctgta agaagtatgc attttccaaa agaccacgaa gaaaaccatc aagccaaacg
    31081 acgtgtcgtt tttgaagagt tctttttatt tcaaatgcgt cttcaaggat tgaaaaaagc
    31141 agaaaaagca gaaacgaatg gtttggagat cctttatgat gtccaaaaat tgaaacaatt
    31201 tactcaaaag ctaccttttg aactgacaaa tgcacaaaaa cgtgtaacga atgaaatctg
    31261 tcgggatctt cgcagtccgc agcatatgca gcgacttttg cagggagatg taggtagcgg
    31321 aaaaacagtg gttgccgcga ttgcgttata cgctaccgtt acagcaggtt tccaaggtgc
    31381 gttgatggtg cctacagaaa tcttggcaca acaacatatg gaaagcttgc aacagctgtt
    31441 tgatcccaat gaagtaacca tcgcattgat tacggggtca acgaaagcaa aagaacgacg
    31501 tgagctcctt gaaaaactag agcaagggga aattaacgtc gtgatcggca cacatgcatt
    31561 gatccaagat ggagtcgaat ttcagaattt aggtctagtt attacggatg agcagcaccg
    31621 ttttggcgtg aaccagcgga aagtacttcg tgaaaaaggc tggcgaccag atgtcttatt
    31681 tatgacagcg actccgattc ctagaacatt agctattaca gcatatggtg agatggatgt
    31741 atctatcatt gatgaacttc cagctggacg tatcccgatc gaaacacgct gggtaagaac
    31801 acctcaattg gacagtgtac tggattggac gaaaaaagaa ctggcaagag gacatcaaat
    31861 gtatgtcatc tgtccgctga ttgaagaatc agaagcatta gatgtaaaaa atgcagtgga
    31921 aatctatgaa aaacttcgtg aactatttgc accaaaatat gaagttggct tgttacatgg
    31981 taaaatgaag aatcaggaaa aagaagtaat catggaagcc ttcaaagaca accagatgca
    32041 gatattagtt tcgactactg taattgaagt aggggtaaac gtgccgaacg cgaccgtcat
    32101 gctgatcatg gatgcggatc gatttggatt ggcacagctg caccaacttc gtggacgtgt
    32161 tggacgcgga agtgatgctt cctactgtat cttagttgct aatcctaaaa atgaactagg
    32221 tgtggaacgg atgaaaatca tgacagagac caccaatggt tttgtactaa gcgaaaaaga
    32281 tctggaatta cgtggtcctg gtgaagtatt tggcttccgt caatctggtc tgcctcaatt
    32341 tgcgattgct gacttggtga cggatggaaa tgttttggag gtagccagag aagaggctgc
    32401 agccctttgg caaataaagg attggcagat gcttcctgag tatcagcctt tagcgcaaag
    32461 cctccaaata gaaaatcagg ataatcgtta tttcgattag cagactgtgc tatactttat
    32521 actgatgacg gaaaaaggag tggaaacaat gaaaattgcc gtagatgcaa tgggcggaga
    32581 tcacgcacct caagcaattg tagaaggcgt ggctttagcg cagcaagatt ttccagaagt
    32641 agaattctta ctttacggaa aagaagcaga aatcaagaaa tacttaacaa atgaaaaaaa
    32701 catcacgatt atccatacag acgaaaaaat caatagtgat gatgagccag taaaagcaat
    32761 tcgacgaaaa aagactgcat caatggtcct tgctgcacaa gcagtgaaaa acggtgaagc
    32821 agatgctgta ttttcagcag ggaatacagg cgcgctacta gcagcagggt tgttcatcgt
    32881 aggacgtatc aaaaatatcg aacgccctgg cttgatgtct accttgccag tagtagggaa
    32941 agaaggtgcc ggtttcgata tgctggatct tggcgctaac gcagaaaaca aaccagaaca
    33001 tttgttgcaa tacggtattt taggttcttt ttatgcacgt aaagtccgag gtatcggacg
    33061 tcctcgtgtc gcactcttaa acaacggaac agaagcaaca aaaggtagtg aagtgacgaa
    33121 aaaagcctat gaactccttc agaatgaaac aagcttgaat tttattggaa acgtagaagc
    33181 tcgtgagcta ctcaatggtg tggctgatgt agtggtcaca gatggattta caggcaacgc
    33241 tgttttgaaa tctattgaag gaacagcaat gaatatgatg aatctattaa aatctgctat
    33301 tctaaatgaa gggatcaaag gaaaaatggg cgctatgctt ttaaaagacg gcttacgttc
    33361 tttgaaagca gaaatggatt attccaaaca tggcggtgcc gttttgtttg gcttgaaagc
    33421 accagtcatt aaaactcatg gagcaacagg ccccgatgct gttcgctata cgatccgcca
    33481 aatccacaca atgcttgaga cagatgtagt tggacaatta gtcgaacaat ttgaaaaaag
    33541 cgaagaatag tatttggata tctgaagcga tccgttccat tgaaaaaaag gtggacattc
    33601 ctgaaaaatg ccagtaaaat tagccatggt gtaggtaatt gacaagtgta tgattcttgg
    33661 aggtgaaaaa gttgactcgc gaagaagtat ttaacaaagt agcgaaaatt atttcaaatc
    33721 attttgagat agataccgat aaagtgacgg atgaattgaa catcaaagat gatttaaaag
    33781 ctgattcaat tagtattatg gaattcgttt tagagcttga agatgaattc ggtacggaaa
    33841 tttcagatga agacgctgaa caaattgaaa cagtcggtgg cgcagtagat tatatcagca
    33901 gccatcttaa ataatttcaa ataaaagcaa agaaggataa aaagctgcat gcagcttttt
    33961 atccttcttt ttttaatctt tttgacacgt aaaagtcatt tttctgataa ttctggcgat
    34021 aaacagaaat tatgttgcaa agtaaagcgg ttttctatat aattattaat aattcattac
    34081 acaaagataa gaataacctt tgttataaaa cgatttattc tgtgtaatgt ccttttttat
    34141 taagaaaaac ttccatttag gaagcagaga ggggtattcg attgtccgat aaccaattat
    34201 tagaagttca gaacctacat acaggattcc gactcaaaga tgactattac gatgcagtag
    34261 atgacgtttc atttacttta gacaaaaacg agattttagc aatcgttggg gaatctgggt
    34321 gcgggaaaag taccttggct actacgatta tgggattact tgatcctaat aacacaaaaa
    34381 ttacaggtga gattttatat aacgatctga atttaatcga cctgaacgaa acgctctata
    34441 ataaaatccg cggaaatgat atcggaatga ttttccaaga tccgttgtct gcattaaatc
    34501 ctctcatgag aatcgaagac cagatcaaag aaggactttc ctatcacaca aagatgaatg
    34561 cagaacagcg tcaagctcgt gcactagaat tgctagaaca agtagggatt cctaatcctg
    34621 cgagagttgg acgccaatat ccccatgaac tatcaggcgg tatgagacaa cgtgtcatca
    34681 tcgcaatcgc aatcgcctgc aaacctccaa tcgtcattgc agatgaaccg acgacagccc
    34741 tagatgtaac catccaagca cagattcttg atttactgaa agaccttcag gaagaaacaa
    34801 aaacagggat catcttgatc actcatgact taggagtcgt agcagaaatg gctgatcgag
    34861 tggcagtgat gtatggcgga cagttcgttg aagtagcagg tgtaaaagaa ctgtttgatc
    34921 atcctaagca tccttataca cgctctttac tgaactctat accacaagaa ggaaatcatg
    34981 atgcagaact ccatgtgatc gaaggtgtcg ttccttctct gaaaaacatg cctcgtaaag
    35041 gttgtcggtt tgcaccacgt attccatgga taccagcaag cgatcatgaa gaagagccta
    35101 tccttcatga agtcgcaccg aaccacttgg tacgttgctc ctgctataag catttccact
    35161 ttagagacga aaaaggagag gtgtagatga cagaattaat cacaatcaaa gacctgaaag
    35221 ttcattaccc tattcgcagt ggattcttca atcgagtaac ggatcatgta tatgctgtag
    35281 atggtgtgga ttttattatc gaaaaaggaa agacatatgg tttagtggga gagtctggct
    35341 ctggaaaatc tactacggga aaagccgttg tgggattaga aaaggtaaca gccggacaaa
    35401 tcatgtatga aggaaaagat gtaacgaagc gaagcaaccg caaaaaaatg ggttataaca
    35461 aggatgtgca gatgatcttc caagattcaa tgtccagttt gaatcctaaa aagcgagtgt
    35521 tggatatcat tgctgaaccc atccgaaatt ttgaacggtt aagcgatcaa gaagaaaaga
    35581 aaaaggtaaa aggactgcta gatatcgttg ggatgccgga agatgcgcta tataaatatc
    35641 ctcatgaatt ttcaggcgga caacgtcaac gtctaggagt agcccgtgct gttgctacca
    35701 atccaaaact gatcgtagcc gatgaacctg tatctgctct ggatctatct gttcaagcgc
    35761 aagtattgaa tttcatgaaa cgaatccagc aagaatacgg cctcagctat ttattcattt
    35821 cacatgattt aggtgtagta aaacacatgt gcgacaatat cgcaattatg tacaaaggtc
    35881 gatttgtaga aatcggtaca agagaagaca tttataatga cccgcgacat atctatacga
    35941 aacgtctgct ttcagctatt ccgcaaatcg atgtagaaaa tagggaaagc cacaaagaaa
    36001 atcgacgaag agtagaacaa gagtatatcc aaaaccaaaa agaatactat gatgcgactg
    36061 gtcgagtcta cgatttgcgt acgatcacaa ctacacacaa agtggcgttg aaagatggag
    36121 gtgcaagcta atgtggaaaa cgattctgcg acgtgtttta ttgatgatcc cgcaagttat
    36181 tattttaagt gttctgatct tcttattagc ccaagcgatg ccgggggatc catttaccgg
    36241 tttgatcaat ccaaatcagg atccggcagt gatcgaacag atgcgacaag cagctggctt
    36301 gaatgaccca tggtatgtcc agtacttccg ttggatcgga aatgctttgc aaggcgattt
    36361 tggtcaaagt ttcttataca aacgctctgt tgctgacttg atcggcgaac ggatcatcaa
    36421 cactttatat ctatcgatct tgacagttgt ccttacttat ttgattgctg tgcctctggg
    36481 gatgcttgct ggccggtatc aaaattcaat cttggataag attgtcgtaa tctacaactt
    36541 ctttagtttt gctgttcctt tatttatctt cggcttgatc atgctatttg tttttggtta
    36601 tcgtttaggc tggttcccaa caagtggatc acaaacacta ggtttgcaag gtggatttat
    36661 cactcaatgg atagaccgtt tgaaatatat tctgttacca tcaattaccc aagcattttt
    36721 ggcgactgcc gtcacaatcc aatatctgcg aagtgaagta atcgattcga aatcgctcga
    36781 ttttgtcaga acagcccgtt ccaaaggggt accgacgaat aaagtattca gtcgacatat
    36841 tttccgaaat gcagcgttgc cgatggcttc tcaaatgggg tatgaaatca cttcgttgat
    36901 tgctggttct gtagttatcg aaaagatttt tggttatccg ggagtcggaa aactatttat
    36961 tgattccatc ggccaacggg actataccgt gatcacatcc ttagtattga ttttaggaat
    37021 cgcgacgcta gtggggacat tactttcaga tatcatcatg agtatcgttg acccgcgtat
    37081 tcggattcaa taaaaccaat cagaaaggag aatggatcga tgagtgaaaa aaacaataca
    37141 gcagttcaag aaagtattcc tcctatgggg atacgaatga ttgctcgaga atttaaaaaa
    37201 gataaaattg ctattttttc attaggtttg ctagtggttt tattattagt gatctttatc
    37261 ggtgcgatgc tgaccgatca agacaaagtg atgtatgtta gtatcttcga caaatacgct
    37321 gcaccaggaa cagtttcgac acaaggacaa aagttcattc tgggagcaga tgaaggcgga
    37381 cgtgatgtat taggacaatt gatcataggt gctagaaact ctgttacgat cggatttgcg
    37441 atcacgatcc ttacttcgat catcggggtt ggattaggga tcatctctgg ctactacgga
    37501 gggatcattg acaacatctt gatgcggatc gttgatttta tcatgattct accgatcatg
    37561 ctgatcatta tcgtcttcgt atcgattatt tccagataca atgtctggtc ctttatattc
    37621 atcatgagtg cattttattg ggtagctaaa gcccgattat tccgaagcaa gacattatct
    37681 gaagcaagaa gagattatgt cagtgcctca aaaacaatgg gaaccaatga ttttaaaatc
    37741 atgttccgcg aaatcatgcc gaatctaagt tcattaatca ttactaattt gacgatgaac
    37801 tttgcggcaa acatcggtat cgagacaact ttgactttct taggtttcgg cttgccggca
    37861 aatgtaccta gtttaggtac attgatcggt tatgcaagta acggagaagt cttagtgaat
    37921 cggcaatgga tctggttacc cgcatcaatc cttattttag ttttgatgtt gagtatcaat
    37981 tatgttggac aagcattcaa gcgttctgca gatgcgcgcc aacggttagg ctagtataga
    38041 agcgaagggg gaaataggaa tgaagaaaac gatttttgga tttgcagcgc tgatgtctgc
    38101 tgtcgttctt gcagcatgcg ggggcggagg tacagatacc tctggaaaca actctggagg
    38161 caacggcaat aacaaagagg aattaacttt tccaattgca acaaaaaata atgatgatgc
    38221 aatcgaagac ggcgaattag aagctgcggt cgcaacggac agtcagttta aaggattgtt
    38281 ccagtgggag ttttaccaag attcatatga tgcagctttt atggctcctt ctcatgaacc
    38341 gctatttgca aacgatgacg atttcagaat cacagacgac ggagctgcaa cgcttgatct
    38401 agatgaagat acgaaaaaag cgacaatcaa agtaaaagac aacgtcaaat ggtcagatgg
    38461 tgaagatgta acggcggacg atgtgatttt cccttacgaa atcattggga ataaagatta
    38521 cactggtatc cgttatgacg acactttccg taacatcgta ggtatggaag aatatcatga
    38581 tggaagtgcc gacacaatct ccggaatcaa aaaaatagac gacaaaacag ttgaaatcga
    38641 atataaagaa gtaaacccaa gcatgcttca atcgggtggt ggtatctggt catatgcagc
    38701 accaaaacat acgttagaag gtgtagcaat caaagatatg gaatcaagcg atcaagtacg
    38761 taaaaatcca gtgacttttg gcccttatta catgagtaat atcgttgcag gagaatcagt
    38821 agaatacttg ccaaacgagc actactggaa tggtacgcct aaattgaaaa agatcacgat
    38881 gaaatctgta ccgatggcaa gtgcaactga agcattgaac tctaaattat atgatatgat
    38941 tttccaaatg ccaacagata cttacgatac ctataaagaa attgctggct atacaaactt
    39001 aggccgtgaa caaacttctt atacttatct aggattcaag ttaggtaaat ggaatgcaga
    39061 tacaaagagt gtggaataca atccagattc aaaaatggct gataaatcat tacgtcaagc
    39121 aatgggctat gcattagata ataatgcagt aggtgaacgt ttctatgcag gtttgagaag
    39181 caatgcgact tcattgattc ctccagtatt ccagtcatac catgatgatg agatgaaagg
    39241 attcacacaa gatctagaca aagcgaaaaa actattagat gatgctggct acaaagatgt
    39301 ggatgacgat ggtttaagag aagacaaaga tggaaataaa ctaacaatca actttgcttc
    39361 gatgtccggt ggggaaacag cgcaaccatt ggcagattac tatatccaac aatggaaaga
    39421 aattggtttg gatgtccaat acacaacagg tcgtctgatc gaattcaatt ctttctatga
    39481 taaattagaa aacgatgatc ctgaaattga tgtttaccaa gctgcttgga atacaggaac
    39541 tgatccaaat ccaagtggac tatggggacc aaatgctgcg ttcaactaca ctcgttatga
    39601 atcagaagaa aatacaaaat tgatcaacga tatcagttca agcaaagcat ttgatgaaga
    39661 gtaccaaaaa gaagcatatc aaaaatggca acaatatgca tttgaagaag catttgcgat
    39721 tccaacgttg ttccgtaatg aagtattgcc agtcaacgac cgtgtcaaag actgggattg
    39781 gtcttatgat gtaccaaacc catgggcaaa tgtttctgtt acagcagatt cacgttcata
    39841 atcactaaaa atgaccgaaa aagggcgatg tcctttttcg gttttttgat atttttctgt
    39901 ttatttctct aatattcagg tcaaggcgct gatttcatga cttctccaat aaacggtgtt
    39961 caaagtccag tttctcggta ttaagtcaaa tttatgcaaa aatatggaaa gccattttca
    40021 caaaattttt tcttcatact cgaaactaaa cgaatttttc acaacttctc gtcaatcact
    40081 aggattatta aagctttact taccttttga ccgaaccatt tcaaatttga caaaaaacca
    40141 gtataataag gaagtatcga aaaaatatcc ataaaaggaa tgtggaaaat ggacaaccag
    40201 ctgacaaaag aactaaaaga aaactataac atcgtttttc acgatctaaa tttgcttgaa
    40261 caagctttta cccattcatc ctatgtgaat gagcatcgca atctgcaatt atcggataat
    40321 gagcgacttg aatttttagg agatgccgtt ttagaactga tggtttctca gtatttattc
    40381 cgtttatatc ctcatttgcc agaaggaaaa ttgacaaaaa cacgagctgc gatcgttcgc
    40441 gaagacagct tgtcgaaatt tgccaaagaa tgtcactttg atcaatatgt tttgttgggc
    40501 aagggagaag agaattcagg aggacgtacg cgaccagcat tgctttgcga cctattcgaa
    40561 gcttttttag gcgcgttata tcttgaccag ggattcgaca cgacccatgc attcatcgaa
    40621 aaagtgattt ttcctaaagt caaagcaggt gctttttcac atgagatgga tcataaaaca
    40681 aaactacaag aagtcttgca aaaatcaggt gatgtatcaa tcgaataccg attgatcaat
    40741 gaagaaggtc cagctcatga acgagtattt tggatcgaag tttatgtaga tgatcaattg
    40801 attggcactg gacaagggaa atcgaaaaaa ttagcagaac aagctgcggc ggaaaatgca
    40861 ttggcagcac tgtaaaagta agtgagggaa agctttgtat ttgaaacgaa ttgaaatagc
    40921 cggttttaaa tcatttgcag acaagacagt cattgatttt gaaaatagcg tcacagcagt
    40981 ggttggacca aatggcagcg gaaaaagcaa tattacagag gctatccgct gggtattagg
    41041 agaacaatct gctaaaagtc tgcgaggcgg aaaaatgccg gatatcatct ttgccggttc
    41101 cgatacccgt aagcagctga atattgcaga agtcacagtg attttagaca atagtgatca
    41161 ttatctgcca ttggagtaca atgagatcag tgtaacccgc cgttatcgac ggactgggga
    41221 aagcgagttt ttcatcaata aacaaagctg cagattaaaa gatatccaag aattgttctt
    41281 agattctgga ttaggaaaag aatcgttctc aatcatttct caaggaaaag tagaagcgat
    41341 tttcagtagt aaaccggaag accgtcgtgg gatctttgaa gaagcagctg gtgtcttgaa
    41401 atacaagcag cggaaaaaga aagcagaaca gaagcttttt gaaacagaag acaacctcag
    41461 ccgcgtccaa gacatcatcc acgaattgga agaacagtta actcctttgg ccgcccagag
    41521 tgaggcagca aaggagtttt tacggctaaa agaaacactg actcaaaccg atgtctcttt
    41581 gatggttgct gaaatcaaga cagcaaaaaa agattgggac aacaaacaag cacagcttgc
    41641 gaaattcaat ctagaattag gcaaactatc tgagtcgatc caagaacaag aatccatttt
    41701 ggcgaagcag cgtaaagaaa atgcgcaagc cgatcgtctg atcgaaaaaa atcagcaagt
    41761 attattagat ctttcggaaa aactgaagca gacagaagga caaaaagatg ttttgcagga
    41821 gcggacaaag catacacaaa aaagctcgca agaatatcaa acttcattag cagaagcaca
    41881 aaaaaaggtt aaacattttg aaaagcttca agaaagcttg atgaaagcag cagcagaaaa
    41941 agaaacagag atccaaaaag ctgaagcaaa tctcataaaa acacagcaag aattagaaaa
    42001 ataccaaaaa tcaacgaaag aattattggc agaattaaga gatcaatatg ttgatttgat
    42061 gcaagaacaa gcagctgtag gaaacgaact gaagtatcta gaacgtcaat atatacaaga
    42121 aacagcaaaa agcaagcaga cgttagccaa gcaatcggaa gtagaagcat cagtagaccg
    42181 attgatctta caaaaagaag aattgacaca aaagcaggcg cagctaaaat catctttaac
    42241 tgaaacaaaa gagaagttag aaatgatcca acaaaacggg aaaaagttcc aagaagcatt
    42301 agcaaaagaa cagcctaaaa tgtatcaatt aatgaatcaa gtccagcagc tacgtgctcg
    42361 taagaaaagc cttcaagaaa tccaagaaaa ctattttggt ttttaccaag gggttcgatt
    42421 ggtcttgcag cataaacagc agctttcggg tattgtcgga gcagtggctg aactgatcga
    42481 tgttcctgca gatttcacgt tagctatcga agcagcttta ggtggggcag cccagcatgt
    42541 catcgttgaa aatgaaaaag atgctcggca agccatcacg tatttgaaac agcaaagagg
    42601 aggaagagca acattccttc cactgacgac aatcaaacca agacaattac cggctcatat
    42661 attgacacaa gcagcagctg tagaagggtt tatagggatt gccagtgaac aagtcagtta
    42721 tcccgatcaa atccaaactg tggttcaaaa cctgctagga acgatccttt tagccaaaga
    42781 cttaactagt gccaatgcaa tcgcgcaaac gatccgctat caatatcgag tggtttcctt
    42841 agaaggtgat gtgatgaatg ccggaggatc catgacaggt ggagcgaata aacgaggaaa
    42901 ccaaggcagt ttgtttgtac aaaatcaaga attgaaacag ttgactagtg aatttgaaga
    42961 agcagataaa caactccaag cacaagaaaa aaaagttcag gagctgcaac aagaaacagc
    43021 tcgtttagcc gaagaacaag aagttttgcg tacacgaggc gaacaattac gttttgaaga
    43081 acaagaagcg acaaatcagc tacaaaacat cataaatgaa cttgaacgtt ttgaaaaaga
    43141 aaagcaaatc tcaacttttg agacacgtga actgcagcag tttatcgaag attacgaaaa
    43201 gcaaacgaac gaattgaaag acaaacaaac agatcttgaa tcgcaacgac aaaaaatcga
    43261 cgaagaaatc aaatcgttaa gtcaagaaag cgaccagatg gaagccagaa gagcacaagt
    43321 acaaagtcaa aaagcacaag aacaagcaga tcttgctgtc ttgaaagagc agtttaatca
    43381 tctgcagatc cagctccgcg gtgcccgagt ccaaaaagca gaggcaaccg aacgtcaaga
    43441 agcgatagaa aagcagttgg cgacattgac agcagacttt tctgatcatg aagtcacaga
    43501 agaaagtttg gaaaaacaaa ttaacgaatt atctgctcag cgagaaacat taaaagccga
    43561 gttggcaaaa gccaaagagc agcgagatag aaggcaaaag gagatcgata agcttgaagc
    43621 agtcttggca gagcgtaatc gtgagcaaaa agcacgactg tcggaacaat ccaaattaga
    43681 agtccaaaaa gaccgagctg aaatgatttt agataatcat ttgacctatc tgcagtcaga
    43741 atatcagctg acatttgaga aagcttccca agactatcaa gaaacaaccg atatcgaaga
    43801 cagtagaaca aaggtttcat ccttaaaaga acagattgaa aaactgggac cagtaaactt
    43861 aaattcaatc gaacaatacg aacaagtcag tgaaagacat acattcttag caacacagcg
    43921 agatgatctg ttagcagcga aaaaccaatt gtttgaaacg atggatgaga tggatgatga
    43981 agtgcggaca agattcaaag aagtatttga agctatccgt caagaattca aaattgtctt
    44041 tccaaatatg tttggcggcg gtaaagctga attagtactg acggaaccaa ctgatttgct
    44101 gaaaacaggt attgagattg aagtacaacc tcctggcaaa aaactgcaaa gtctaagttt
    44161 gctttctgga ggagaacgag cacttacagc catcgcgttg ctcttttcca ttatccgtgt
    44221 ctgtcctgtt ccgttttgta tcttagatga ggtagaagca gcgttagatg aagccaacgt
    44281 gaaacgtttt ggtcgttact tgagcgattt ccaagatgac acacaattta tcgtagtcac
    44341 acaccgcaaa ggaacgatgg tcgcagcaga tgtactgtac ggagtgacga tgcaggaatc
    44401 cggtgtatca aaaatcgttt ctgtacggat ggaagatatc aatgaagagg cgaaaatcaa
    44461 cgaaacgaaa gtgtaggaac gaaaatgatc aaattagtag caatcgattt ggacggtaca
    44521 ttattaaatg aacaaaagct gatttcagat gagaataaac aagcattagc acaagcaaaa
    44581 cagcaagggg tcaagatcgt tctatgtaca ggacgcccct tagctgcgat ggcgcattat
    44641 ctgcaagaat taggactggt agatgaaggt gactacagca tcactttcaa cggcggtttg
    44701 gtacaaaaaa atgatactgg tgaaatcatg gaaaagaaag tgatggaagt atcagatatc
    44761 caacgcttat acaaactggc tcagcaattg gatctgccac tagatgtttt gtctgacaat
    44821 gtggttttac agctgcctag tgccccaagg aagcaatcct tgtacaatgt gttgaataag
    44881 ctgctgcaat tccaaccagc ggatataagc gatattacag atgaatttgt gcttaataag
    44941 gcagtaatcg cttatgacca agaagaatta gatcctaaaa tcaaagaaat accagcagaa
    45001 ttccatgaaa tgtatgagat catcaaaaca agaagtatgc ttttggaatt tatgccaaaa
    45061 ggtgttacaa aagcttatgg tatttcttta ttggcaaaag atcttggttt gaaacaagaa
    45121 gaaatcatgg cgatcggtga tgaggaaaat gatttaccga tgatccaata tgctggacta
    45181 ggcgtagcga tggagaatgc tgtgcctttc gtcaaagaag cagcagattt cgtgacagcc
    45241 agcaatgaag aaaatggcgt agccaaagtg atccaaaaat ttgtattaga gtagggggag
    45301 tcaaatggga ttttttgata aaatcaagcg tgcttttact ggcgaagaga aaacagaaga
    45361 agttgaagta caggagaagt acgacaaggg tttagaaaag acgcgtaaaa catttggtga
    45421 acggatgaat gagttgtttg ccaattttcg aacagtcgat gaagactttt ttgaagaatt
    45481 agaagaaaca cttatcggtg cagatgtagg ttttgaaaca gcgatacgaa tcaccgaagc
    45541 attacgtcaa gaagtgaaac tcagaaatgc gaaaaaatcg gcagaagtcc aaaatgcgat
    45601 catcgagaaa cttgtggatc tctatgaagc ggaaggaatc aatgaagtaa atgaactaaa
    45661 tatccaagaa gacggactaa cagttatttt gtttgtcggt gttaatggtg tagggaaaac
    45721 aacaagtatt ggtaaacttg cccatcagtt caaaaacgaa ggtaaaaaag tattgatggc
    45781 agcagcagat accttccgcg ctggagcaat cgaccagtta gttgtctggg gcgaacgcgc
    45841 tggtgttgaa gttgttcgtg gcaatgcagg aggcgatcca gcagctgtcg tattcgatgc
    45901 tttggaaaga gcaaagacag agcaggcaga tgttttacta gttgatactg caggccgact
    45961 gcaaaataaa gtgaacttga tgaacgaatt agaaaaaata aaacgagtaa tccagcggga
    46021 attaccagaa gcaccccaag aaacacttct tgtattagat gcaaccactg gtcaaaacgc
    46081 gatggttcaa gcaaaacaat tcaaagaaac aacagatgtg accggtcttg ttttgacaaa
    46141 attagatggt acagcaaagg gcggtatcgt acttgctatc cgtaatgaat tgcatttacc
    46201 agtgaaattg gtcggtcttg gtgaaggaat cgatgacttg gagccgtttg atccaaatga
    46261 ttttgtcgtt ggattgttca aaggattact aaaagaagaa taacccatgt ctaaaaagca
    46321 ggagctgtga caaaagttgc agctccttgt ttgaatgatt gtttttaaca tagctgtgtt
    46381 tttgatgcct tcatttttat acttatttgt aagcggttct caaacaattt cgttcttttt
    46441 cttagacttt ttgattataa ttagattcat atgaattgga ggtcacttaa tgatgaattt
    46501 taagcaatta ttattgcatg tcaatgcgcg cccttttatc gaccaagctc gatttggtat
    46561 agaacgggaa ggacagcggg ttgatcttgc aggaaatcta gcaaaaaccg atcatccagc
    46621 aatctttggc gatcgatcct atcaccccta tatccaaaca gattttagcg aaacacaaac
    46681 agagatgatc acccctgtta ccgattctat tcccgaatta tttcagtatc tcgctgctgt
    46741 ttatgatgtg actgctcgtt ctataccgaa agaagagatg atctggccat taagtatgcc
    46801 acctgcctta ccggaaaaag acgaagagat cattattgca aaattaaaaa atttcgaaga
    46861 tgtcttgtat cgacgttatt tagcaaaaga atacgggaag cgaaaacaga tggtaagcgg
    46921 tattcatttc aattttgaat ttggcgatga gttgctaaga acattgttca gccatcagga
    46981 agaatttcaa gatttttccg aattcaaaac agaactttat ttgaaaacag cgagaaactt
    47041 tatgcgctat cgctggatga tcacttattt attcggtgct tcaccaatga gtgagaagaa
    47101 ctacttttta gatgaatcac atccgcaaga acctgttcgc agtattcgca acagtgcgct
    47161 gggctatacg aatcatccaa atgtgaaagt ttcctatgct tctatgaaac agtatttagc
    47221 agatatcgag cgaatgatcg aagaagggaa actttcggag gagaaagaat tttatacacc
    47281 gcttcgtttc cgaggcggaa agaaagtcgc agatttagca acaacaggtg ttcgttatat
    47341 tgaattgcgg aatatcgatt tgaatcctta tgcaagattg ggtatcaatc cagagcaagt
    47401 tcggttctta caattattcc tgatgtacat gttatggaca gaagaaaaag aagactgtga
    47461 ccaatgggta gcagaaggta cgactcgaaa taacaaagta gcactagaac aaccatcaga
    47521 tcaaacagaa tttcatcaag aaggtagaga aattcttgaa ggaatgaagc aaatgctggt
    47581 tgaattggat tggttggatt ctctttattt agtagaggaa gcattgactc aaatggatca
    47641 tcctgaacaa acgttagcag cgaaactcta tcaagaagca cagctatcga gtcagcaaga
    47701 agttgctgtg gcattgggac atcaatatta taaagaaagc catgaacgtc cctatcagtt
    47761 ggccggtttt cgcgaaatgg aactttctac tcagatattt atgttcgatg ccatccaaaa
    47821 aggtgtccaa gtcaaagtat tagatgagtc ggatcaattt ttgcgtctgc aattccaaga
    47881 ccatgtagaa tatgtgaaaa atgcaaacat gacaagtaag gacagctata tcgtcccact
    47941 tatcatggaa aacaaaacag taacgaaaaa agtcttaaaa gaagctgggt tccgggtacc
    48001 aggaggcgca gaattttcat ccatggaaga agcggtaaaa gcttatccga gatttgccga
    48061 gcaggcattt gtgatcaaac ctaaatcaac caattacggg ttaggtatca cgattttcaa
    48121 ggaaggagct agtctggaag attatcaagc aggtttagcc attgcttttc gtgaagacag
    48181 ttcggtttta gtagaagaat ttatgccggg aacggaatat cgtttctttg tgatcgacgg
    48241 agaagtccaa gccatcatgt tgcgagtccc tgctaatgtt attggtgaca gtatccgaac
    48301 agtgaaagaa cttgttgaag aaaaaaatag tgaccctttg cgtggaacga atcatcgtgc
    48361 acctttagaa ttgatccaat taggtgaatt agaacaattg atgttgaaag aacaagggtt
    48421 gacgatcgaa tcagttcccc aagccaatca aattgtttat ctaagggaga actcaaatat
    48481 cagtacaggt ggtgattcga tcgatatgac tgatgaattt tctgaagctt acaaaaaaat
    48541 cgccgtttct gcggtagaag cactaggagc taagattagt ggtatcgatt tgattatacc
    48601 agataaagaa atcgatccta caactgataa aaaagcatat gggatcatcg aagcaaattt
    48661 caatccagcc atgcatatgc atgtttatcc ttttgcagga aaaggcagaa gattgacgat
    48721 gaatgtattg aaattgttat atcctgaagt attttaaaag aaaaagcttt tgacagattg
    48781 tcctatttga tgtcaatctg atcaaaagct ttttttgcat gtaaaaattt atgtgttttt
    48841 tcgttttaaa ataaacgatc ccagaagctt tttttctttt ttggtttttc tttttcagct
    48901 ggcgaagagt tttcttctgg gtattttttt tcgatatcaa tcacaggttc atttactgct
    48961 tcttttgctg ttaagagcaa tccgaattcc tcaggatttg cagtgacgta atcgttgact
    49021 acagtgaaac ggctattgtt tttcgttgct agctggatat attggttttg gacagaaagg
    49081 ggtagagccc cattcaagag tatggaagca tctggatgtt ttttgagttc ttttaaaaag
    49141 ttttctttgt tttttggttg gatcatttca gcaacagtca tactaagata gcagcgttcc
    49201 ctgaatgtcc ctaaatattt atgctgttct tcagggttca ctaaaggtgt accataaagt
    49261 cccttatcca aatggctttt tacatcatct tgtgccataa aaaataccct ccttcttctc
    49321 tttgtctttc ttacgattat agcatattcc caataatacc taaaacatat gaaaaagcgg
    49381 ttgcttttgt tcaacgattc acttaaaata agaatgaaaa tcattctcaa ttagaaaggt
    49441 ggattggatg cgctacggac gactagtagc attattagtt attttaagta gtatttcatt
    49501 gttcatcgga gtatcgaatg tacaattgtc agaactgctc cagttagatg ggcaaagctt
    49561 acaaatcttg ctagtgactc gtttgccacg aacgatttgt ttgatccttg tgggagcgac
    49621 cagtagtatt tgtgggttga tcatgcaaca tttgacccaa aataaatttg tttcaccaac
    49681 gactgcgggc acgatggata gtgcgcgttt aggtatttta gtggctatga ttttcctacc
    49741 tggtgcatcc ttgattgtcc gatccttgac agcattttgc tttgcttttg caggaacatt
    49801 gcttttttta tcccttacgc gattgttcct ccagaaaaat caattgatgc ttccgttgat
    49861 tggagtcatg ttagggaata tcattggttc agttgctact ttttttgctt atcaattcca
    49921 gttggtacaa aatatgactt catggcttca agggaatttt gcgactgtga tacgaggaaa
    49981 ttatgagtta ttgtatgctg ttgttccatt gttgctcttg gtttggctat ttgcttaccg
    50041 atttacgatc attggtctgg gcgaatcgtt tgcagtaaat ctgggaatag attaccagaa
    50101 gatgcagttt ttaggcattg gcttagtatc attggctagt gcagtcatgt tgatcatggt
    50161 aggaagcatt ccttttatcg gagtgatcat tccgaatctt gtctctagaa tatatggaga
    50221 tcaagtgcac aaaacaattg gcgtaactgc tttagcaggt agtttgttct tagtattttg
    50281 cgatcttgtc gctcgcgtgt tgatttttcc ttatgaaata ccagtcagtg tgatcgttgg
    50341 aatcatcggt ggggctatct tcctttatct tctgataagg ggacgacgat atgcttaaaa
    50401 aaaagccatc tatttttatt ggtatgcttg ttcttgtttt atttgttaca ggaatgtact
    50461 tgacgatcca tacatatgga aactggcatt ttgcatgtgc attgaggagt aagaaaattt
    50521 ttgcatttat ccttgtctcg ctagctgtca gcagtgccac aatcagcttt cagacaatta
    50581 cgcataacca gtttttgact ccttcagtat tgggacttga gtcgttatat gtccttgtcc
    50641 aaacggtcct tttcttcttt atcggaggag tgacgatgct tcagcagaca ggtacagttt
    50701 ttttcttatt gaacgttggg ctgatggtcg ggctgagtct attgttattt ttctttcttt
    50761 tgaaaaaaat gagagcgaat ctttttttat tactgatgtc agggatgatt ttaggtacgc
    50821 tttttggtag tgtcagtacc tttttacaag tcatcatgga tccgaatgaa tatgatttgc
    50881 ttcaaggcag attatttgca agctttggca atatccagag tcagtatctt ttgcctgctt
    50941 ctttgatcat tatcggctgc tggtacctgt tatttcgata tcgacatgtg ctagacgttt
    51001 tccatctcgg aaatgaacaa gcagtaaacc tcgggataga cacagatcgt tttgaatttt
    51061 tgattttatg ttgtgtcagt cttttgacag ggacatcaac ggctcttgtg ggtccagtta
    51121 cttttttagg gtttattgtc gcaaatatca gttatcgtgt cttttctact tatcgtcatg
    51181 gtcagctatt tatcggaagc tttctggtag ctatgctgtt tctgacagga ggacagctcg
    51241 cagtagagca gattttcggc tggaatacaa cagtgagtgt cgtcgttgaa tttgtcggag
    51301 gaatttattt tgtcacaaaa ttgattcgcg aaaggaagag tatcctatga ttgatttaca
    51361 aaaggtcaat aaactgtacc atggaaaatc agtggttaaa gatgtacagt taacgattcc
    51421 tgaaaagaag ttgactgctt ttatcggtcc aaatggtgct ggaaagtcaa cggtgttgtc
    51481 gatgatcagt cgtttgatac caaaagatac aggtgaaatc tatttagacc ataacgaagt
    51541 aaaggcttgg aaatccaatg aattagccaa aaaattatcg atcctacgac aatcgaatga
    51601 aataaatgtc caaatcaccg tgaaagaatt agtagaattt ggacgatttc cttatacgaa
    51661 aggaaaattg acgacaaaag acgaggaaat cgttcaggaa tcactggaac accttgggtt
    51721 agaagaaata gcggatcaat cgattgacac tctttcaggc ggtcaactcc aacgtgcgta
    51781 tattgcaatg gtgctagcgc aagacacgga ttatatttta ctggatgaac ctttgaacaa
    51841 tctagatatg aattttgcgg ttcaaatcat gcagatttta aaaaatttag tacaagaaat
    51901 cgggaaaaca attgttattg ttcttcatga tatcaacttt gctgcaagtt atgcagacga
    51961 aattgttgca atgaaagatg gacagttatt tacgcaagga acgacccaag aaatgataaa
    52021 aaaagatgta ttggatgaat tatacgaaat ggacatacgg atttgtgaga ttgaaggccg
    52081 cagattctgt ctttatttct aaaactaaca ttaggagaga agagatgaaa aaattagtag
    52141 taggtatctt gattctagca tcatttggtt tagcagcatg tggaaatagc aacaacacca
    52201 gtcaggcaga cacaaaaaaa agcagcacac aaactgaaac tacattgacg ataacagata
    52261 gtaatggaga ccaaatcgag gtgccaaata atccagaaaa agtcgtcgta ttcgacaatg
    52321 gttctttaga tacgatggat gctcttggtg ttggagataa ggtagttgga gcagctacaa
    52381 gcagccttcc tgagtatctt tcatcctata aaaaagtaga atcagcaggt ggaataaaag
    52441 aaccagactt agaaaaaatt aatcagcttc agccagatct aatcatcatt tctggccgac
    52501 aaagagattt tcaaaaagat ctaagtcaaa ttgctccgac cattttcctt tctttagatg
    52561 cgaaaaatcc atgggaatct ttccagcaaa atgtcactgc tttaggggag atctttggta
    52621 agcaagaaga agctaaaact caattagagg aattatctag tgcaatcgat cagacgaaaa
    52681 agaaagcaga agctactgac aaaaaagcac tagtcacact tgtgaatgaa gggcaattat
    52741 cagcctacgg atctggttct agattcggtt tcatccatga tttatttggc tttgaacaag
    52801 cagatgatca aatcgaagca tccactcacg gtcaaagtgt ttcttatgaa tatgtgctgg
    52861 aaaaaaatcc ggatatctta tttgtagtag acagaacaaa agctattggt ggagacgaca
    52921 gcaaagatga tatttctgcc aatgaactgg tagcacaaac gaatgctggt aaaaatcagc
    52981 agatcatctc ccttgaacca gatgtctggt atttaagcgg gggcggttta gaatcaatga
    53041 aactgatgat cgaagatgtg aatcaagcat ttaaatagca taaataacag aggattaaac
    53101 tctcatacta tgtaacaata atgtttctta cattcttaaa tagcaagagc ctatcctcaa
    53161 agaagttatc aaaaaccatc cgtaaaatcg tattgatgat tttacggatg gtttttattt
    53221 atttgagaaa tcttttttca acgtgaattc attatctaca tcatacgggt tgattctaga
    53281 atggggagtg gcaaaaaata gtgtgatcca ttttttcagc atcaagatcg cctcctttga
    53341 tttgaatcaa aggtacaaag cgaaacttag ataaaactta aatttagata tttttatttt
    53401 gtaccgatct cttctttaaa cgatgttcca tctgctgctt ctcggcaata agtccactag
    53461 actagaaaat atgagaagct attttctgtc cagcgttctt attactcaaa acaaatcgca
    53521 gttgtcacaa tctcttctaa acgatgttcc atctgctgct tctcggcaat aagcccacta
    53581 gactagaaaa tatgagaagc tattttctgt ccagcgttct tattactcaa agcaaatcgc
    53641 agttgtcaca atctcttcta aacaatgttc aatcagctga ccttcggtat tagctcaaag
    53701 cactgatttc acaacctcta tttaggcatg tgtctcagca agcggttgat agttgatgat
    53761 ctgcattttt cgataaaaat cggcgggaag cttactccac cgttttagta attgatagaa
    53821 ataactctct tctgctttga ttttatactc gcttaatatc aatccaaaac tattcgtttt
    53881 ttttgaagct aattttttat aaatatccaa tgctttggtt ttatcttcat tgacaaagag
    53941 tgtttttact tcaatataag aaccctcgat attttgtgtt tcttcgataa aatagatgtt
    54001 ttttatatat tccttcatag tgattcctcc aaagaagtct tttatttgat aagtttactc
    54061 taccgaagag ttgttaataa tccgtgaaca aaaagtaatt ctttttccaa tagttgttaa
    54121 aggtttttta ttttttatag tattttttgt tatttaagaa aggaataagg ttaaacaaat
    54181 aacaggttgc tttttgggtc tttttccgtt caagaaactg aacgaagaaa agggaaagtt
    54241 gtctcttttg tgataagaga aatcataata aacgaatgac ttactttcca ataaattgag
    54301 aacagtcaga accagacaaa ttagttgcat ctgatgaata aagtgattat tctatgacat
    54361 gaggtgatac agatgaaatt agaagtgaca aaagaagcac aagaagtatt gaagaataaa
    54421 ttagaagcag acgatcaact tttattagat atcgaaaacg gtgacggtcc atttgcagaa
    54481 agccaagtca gttgccaatt ggatacggca tttcgattga tcattgtcaa aaaagataca
    54541 caaacagatc taagtcttta ttctgtagtg gctgatacac cagttggacc aatcaaaatc
    54601 aaagacagtg caatacttta catggatgat ccatccacac ttgctttaga accaacttat
    54661 aatagtttac aattaaaagg accaagcggc ttgagaaaag gcaacttgca agttgttcga
    54721 aaagactaga gggataaaat gggaaaattg gcgatcatca gcgacctaca tgtggacatc
    54781 aataaattcg gcgaatcaga gctgatgcag ctttggcaag ttttgcaaaa gcacaagatc
    54841 actcggcttc atctagccgg agatacggct aataaagtag accgttgcct agctgttgtc
    54901 gaatttttcg tcaataaagg attatctacg acatttcatt tcggcaatca cgagttggca
    54961 gatgtgccca atgaacagat gatggaccat tttccagacc cacatttttt aaatgaacgg
    55021 tatattgctt taaatgagca gaccgttctt ttagggatga atggctggta cgactatcag
    55081 ttttccgaat tgaaagatta cgaggcaatc gtacgaatga agcgtttgta ttggtatgat
    55141 cggttcatcc agcgtgggaa aacagatctc gaggtcaatg cagaaatgct tgcagctgca
    55201 aaaaatttgc tagaccagct ggaagcaaaa aaacttgagg tgattttatc cactcatttc
    55261 gttcctaaac gggcatttat catgtatcaa tctgctcctt atgagcgttg gaacaaatta
    55321 aatgcatttt taggatcaga atcattcgga gaattacttg accgttattc aaatatcaaa
    55381 caagtagttt ttggccatac gcatcgtcgc tttgaagata aatccatcgg tcacacgatt
    55441 tacagctgca gaccttttgg ttattattat gaatggcagc tgacccgaga ttttgtcttg
    55501 aaagaacagt tgctagacag ctatgatcct atgaaattac gtgtcctctt gcgtgcccat
    55561 cacccagctt ttcaggccta tcaaacacaa catttgcaag aggaatttga acaagcgatg
    55621 acaatcgttc cgtattgaga cagcattagt aatgtgtaaa agaaaatacc ttgtaaaaca
    55681 taaggataag taagagcagt agatccattt atcgtggata tgctgctttt tttgcggaaa
    55741 ataatcagcg aatgtttatt tgatcggatg atttgtaact cgcagagtac tagatgcaag
    55801 cgtgtattct ttttttgaac gataatcaca aaaaaaaata taatatacat tttttattta
    55861 attataatta atattgattg aagtctaggc agtcaagtca cattttgata gataggaggg
    55921 ggacttacga attattttaa aggctatgac agctgtattt ctagattgtg tttgcatcaa
    55981 gttgcaataa aaactagaaa tcaaatcggg ctgataatca tttataacta atcgatacat
    56041 aggaccaaat ataggtctat gttcttttat ttcataagta tcgtaaaaca gaattttgct
    56101 atggattaat ccaatcgact gatatgttta gtaaataaag aaagaggaat gtcaatgatg
    56161 aaaaaagaaa tcgtattaac agcagcaatg atgttattca gcatggtagc aagtacgact
    56221 tttgtttctg caactgaagt atacccaaag gaatataaca cagaaggaac gattacattt
    56281 gaagcaggag atgagggtgt aactcctcct gttgatccag agaatcctga tcccaataaa
    56341 cctgtcgatc catcagatcc tccaagccct ggaacaggag gggcattatc gattgattat
    56401 ggatcaaaat tcaaatttgg gactcaaaaa atttctactg cggataaaac gtattatgca
    56461 gctgctgatg tgatgaacga tgggtctaga aaaccgacat atgtccaagt gacagatagg
    56521 agaagtacct tatctgggtg gaaattaagt gtctctcaac cagaacagtt taaaactgcc
    56581 agtggggatg aactagttgg agcacagctg aaatttacga aagggcaggc tgtttctttg
    56641 gttgatccaa catatacgcc gcaaacggtg aattctgaat taacgttgac accgggagga
    56701 aacaatacgt tagcaattaa tgctaaatca ggaacaggtg tggggacttg ggtctatcga
    56761 tttggtgcaa atgaaaatga aaatcaagat gcggtacagc tttcggtacc cggtaaatct
    56821 gtcaagttag cgcaacaata ttccactaaa ttagtttgga cattagaaga tacacctaac
    56881 aactagttta ctgaaaagaa ctgttaaaaa taaatacgta gtcatattgt cttgcgaggt
    56941 aatcgagcaa aaattgtcaa aggctaggct aaatttgttt ggatacgaat agttcatcta
    57001 ggaatttaac gatcaggagg aaaagatata ttgaaaaaga ggaggatcgt gctcatttta
    57061 agcagtctct tagtgactaa tatctttccg atcatagggt actctttgga attgaatcag
    57121 ttggagcatg gaaatagaag agacgaagaa agcaaactca cttcgacatt cgacagttta
    57181 atagaatcca accagacaca agaaagcagt gaggatttaa gtatttcgag tagtggagag
    57241 accactgaca acgtagaagc tgagggttcg gacaaacaag gactagaaga tgcaccaatt
    57301 tctgcggttg agctgcctga cgatcccaat attatttcta tcgataaaat ttttcaaaca
    57361 cctatcggta ctagcacgtc catattggaa aatgggaagt tactgcagtt gaacccagca
    57421 gaaaggtctc aaagaggagc tatttggtca aataagaaga ttagtttact ctcggatttt
    57481 acatttaaat cttatttata cttaggaaat caacgagata gagcgggaga tggcatgacc
    57541 tttacattga ctggcgatcc tagaatggct agtaatccca gtcaagttat tggatcgcca
    57601 ggtatgggaa ttggctcata ttctactcgt tctggccaac cttacattgc gaatgcgctc
    57661 tcgattgagt ttgatacgta taaaaatagt ggttcttctg atcgaatgga tcgagagata
    57721 actgctgata atagagggca tggacatgtc gcttttgtca cgcctaaacc aaataataat
    57781 aattattctg gagagcatac tggcgtgacg gtagctccta catttctttc taatggcaca
    57841 tggcgcttgt taataattaa atgggacgcg gagcaaagaa gtcttactta tgatttggaa
    57901 ggtgtcggtc gctcttctcg gattatacct gatctcatta ggcagtttgg ttctacagaa
    57961 gtaaattggg ggtttacttc ttcaacaggt actttttttc aagaaaatgc tgtcgctgtc
    58021 acgcgacttc catcgaatgt cacatcaaca gctgatgtaa aagtcaacga tagtgattat
    58081 ggggccatcg ctgaagctgg aggaaacgat aagataacta tcagaaatac attgaaggtc
    58141 gagggggatt ttcttgaata taagaaaatg cagactagta tcgacctccc acaagaatta
    58201 actattcccg agcatatctt gatcaatgga aatcaaattg atgccaaaga tatcttgata
    58261 accggaaatc gagtcacgct aaatctgaca gattatttta ttgctggaga tacgttaata
    58321 atcgaattgg agacaacttt gcaggaagta attcctgaaa aagtactcac tatgcatttt
    58381 gaataccttg aaaacgggaa tttgattgag caatcgaatg aaataaacct aaagatcgca
    58441 aagctgaagg agatagatat ccatgtttat tataaagata aagacagtca gattgatatt
    58501 gctgaaatga aaacaatcac tgggaaaata ggagagaagt ataaagaaac tcctattgat
    58561 attgaagggt atgtttttga ctcggattca gcgaatacag aaggcatctt ctccgaaaat
    58621 acagaggata tctattttta ttacagaaaa ggcaaacttt atctctcaac tgctcctcaa
    58681 tatgtcaatt ttggaaaaca taaaatatcc agtagaccat taagtgaatt cggacagttt
    58741 tcaggtgggt tgaaaattat agatgaacgg gcttttggaa gttggaaatt acaattgaaa
    58801 caagagaaag ttcttacaaa tggagatttt gagcttccag aagctctttc ctttgttacc
    58861 tctgaaaata caacggttat agggacatcc gctgttacga ttttcgaaag tgatcaaaag
    58921 ggtgaaagcg atttgtcctc tcttttagat tctacagctc atcgtggaat cagaataaat
    58981 attcctgtag aaaaccaaag aatcggaacc tttgagggga aactatcttg gatacttgca
    59041 gatgtcccag gaaattaaca ggaagagaag aatataacaa aattattgag tttcaagaag
    59101 ggcacaggta ccataggctc ttgtgtccca cagagacaag attttgttat agtcttctct
    59161 tacatatgat taaaggagcg attttcttga gaccaaaaca acaattattt ataacactcc
    59221 tagttatttt aggggcactt tttaacaata tatcagtgtc agctagtgca tttaattttt
    59281 cagtgacgcc gataacttca gagaatcaaa tcgacaagag aaaaacttac ttcgatcttc
    59341 aacttgatcc ggatcaggaa gtagaagtaa aagcggaatt gagaaatgat acagaaaaag
    59401 aagtgaaaat cgatatttct gtgaatagcg caaccaccaa ttcaaatgtg atggttgaat
    59461 acggaaaaaa cgagattgaa aaagacatga gcctgatctt tgatctggta gattatgtga
    59521 gctatccaca gatagtgacg ctaaaaccta aaagtgtcca aaccgttgct tttcatgtca
    59581 ggatgccgaa tgagcgattt gatggtgtat tggctggtgg gatcacattc caagaaatcc
    59641 agacggaaaa ggaccaaaca gaaactaagg atcaaagctt atcgattgag aatgaataca
    59701 cctatattat tgctttgttg atgcaacaga cactgaacga agttgctccg aaccttactc
    59761 ttcatgaagt taaaccagat caaatcaatg caagaaatgt catcttagca aacgttcaaa
    59821 atgatcaaaa aacctatatc aatcaagtag ttattgaaac aaaaattaca aaaaaaggac
    59881 attctgaagt tttatatcaa gaggaaaaag aaggattgca gattgcgccg aatacaaatt
    59941 tttcttttcc aactgcttta aatggtcaac cgctaacccc aggtgaatat caccttacaa
    60001 tgactgtttt aggaaatgaa aatgaaaatg gaaaattttc gagaaaaaaa ggaaatacta
    60061 cgatcaacta taccaatcaa tgggtatttg aaaaagattt tacaatagac ggaaaagtag
    60121 ccaaggaatt gaacacgacg gatgttacga ttaaaaaaga caataattgg atttatatct
    60181 tagtagcttc acttttctta ttgtttattt tattatgcat acttttgttt gttttgagaa
    60241 aaaaagaaga gaaagaaaag taaggttaag ataacaaata gtaaattttt attattgtag
    60301 gatggttggg ctctttttat aacaatataa aatataccat tttattaata aaaatgcata
    60361 ctagtaaatg atataattct aataacaggt ttgatcgcga gaaaggccaa ataaaacgct
    60421 ctccaaaatg ttaaaaaaaa gacaggacga tgtatattat acaaacaaga atcgctgaca
    60481 acagtgactc ggatccagga aatcagtttt atccccagct agttggtgaa tgcatggaaa
    60541 gtaatatacg tagaaagagc tgatgactaa tgaattatga gtttttttta agcaaagaag
    60601 atcacagaag ttattcatta cttaaatatt tagaagcaag ctcaaattta tcagagtcaa
    60661 tatcgaatgt tcaagaagag ttgtcgctat cgacatttct cttgaaaaaa acgatcgata
    60721 agctgaaaca tgatttacaa aagttcgatc tagagaaaaa tttgcgagta accgtcagtg
    60781 aattggatat atgtctggaa attaatggaa aatgttctag caaagctctg ttatctcgtt
    60841 atataacgga ttctttatct atgaaaatgg tattggcttt ctttcaggaa aaatatgtct
    60901 cagtagaaaa ttttgctaaa cagcatcacg tgagttattc tgtggcttat aaagtattac
    60961 aaagactaaa aagaaatcta aaaaaatatc aaattttttt tgaaaagaga aagttg
//
LOCUS       DAHHPA010000004        60802 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.004, whole genome shotgun sequence.
ACCESSION   DAHHPA010000004 DAHHPA010000000
VERSION     DAHHPA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.004"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            157..1089
                     /locus_tag="NBH55_000218"
     CDS             157..1089
                     /locus_tag="NBH55_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508236.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1310..3073
                     /locus_tag="NBH55_000219"
     CDS             1310..3073
                     /locus_tag="NBH55_000219"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCD1508237.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3157..3615
                     /locus_tag="NBH55_000220"
     CDS             3157..3615
                     /locus_tag="NBH55_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCD1508238.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3724..4407
                     /locus_tag="NBH55_000221"
     CDS             3724..4407
                     /locus_tag="NBH55_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCD1508239.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4554..5168
                     /locus_tag="NBH55_000222"
     CDS             4554..5168
                     /locus_tag="NBH55_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCD1508240.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5184..5390
                     /locus_tag="NBH55_000223"
     CDS             5184..5390
                     /locus_tag="NBH55_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508241.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            5931..7061
                     /locus_tag="NBH55_000224"
     CDS             5931..7061
                     /locus_tag="NBH55_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCD1508242.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            7153..7908
                     /locus_tag="NBH55_000225"
     CDS             7153..7908
                     /locus_tag="NBH55_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD1508243.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            7966..10005
                     /locus_tag="NBH55_000226"
     CDS             7966..10005
                     /locus_tag="NBH55_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCD1508244.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLLYLNQNYQ"
     gene            complement(10114..11265)
                     /locus_tag="NBH55_000227"
     CDS             complement(10114..11265)
                     /locus_tag="NBH55_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCD1508245.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(11419..11949)
                     /locus_tag="NBH55_000228"
     CDS             complement(11419..11949)
                     /locus_tag="NBH55_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCD1508246.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(12048..12398)
                     /locus_tag="NBH55_000229"
     CDS             complement(12048..12398)
                     /locus_tag="NBH55_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCD1508247.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(12555..14084)
                     /gene="ahpF"
                     /locus_tag="NBH55_000230"
     CDS             complement(12555..14084)
                     /gene="ahpF"
                     /locus_tag="NBH55_000230"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCD1508248.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSIRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(14094..14657)
                     /gene="ahpC"
                     /locus_tag="NBH55_000231"
     CDS             complement(14094..14657)
                     /gene="ahpC"
                     /locus_tag="NBH55_000231"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCD1508249.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(14801..15463)
                     /locus_tag="NBH55_000232"
     CDS             complement(14801..15463)
                     /locus_tag="NBH55_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1508250.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            15647..17293
                     /locus_tag="NBH55_000233"
     CDS             15647..17293
                     /locus_tag="NBH55_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCD1508251.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            17284..18366
                     /gene="uxuA"
                     /locus_tag="NBH55_000234"
     CDS             17284..18366
                     /gene="uxuA"
                     /locus_tag="NBH55_000234"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCD1508252.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(18474..20027)
                     /locus_tag="NBH55_000235"
                     /pseudo
     CDS             complement(18474..20027)
                     /locus_tag="NBH55_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            20175..21316
                     /locus_tag="NBH55_000236"
                     /pseudo
     CDS             20175..21316
                     /locus_tag="NBH55_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(21442..22521)
                     /locus_tag="NBH55_000237"
     CDS             complement(21442..22521)
                     /locus_tag="NBH55_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1508253.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(22624..23064)
                     /locus_tag="NBH55_000238"
     CDS             complement(22624..23064)
                     /locus_tag="NBH55_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1508254.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(23121..23531)
                     /locus_tag="NBH55_000239"
     CDS             complement(23121..23531)
                     /locus_tag="NBH55_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCD1508255.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(23711..24145)
                     /locus_tag="NBH55_000240"
     CDS             complement(23711..24145)
                     /locus_tag="NBH55_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1508256.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(24245..25093)
                     /locus_tag="NBH55_000241"
     CDS             complement(24245..25093)
                     /locus_tag="NBH55_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD1508257.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            25482..27248
                     /locus_tag="NBH55_000242"
     CDS             25482..27248
                     /locus_tag="NBH55_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCD1508258.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            27350..28609
                     /locus_tag="NBH55_000243"
     CDS             27350..28609
                     /locus_tag="NBH55_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD1508259.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            28756..30057
                     /locus_tag="NBH55_000244"
     CDS             28756..30057
                     /locus_tag="NBH55_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1508260.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            30060..30911
                     /locus_tag="NBH55_000245"
     CDS             30060..30911
                     /locus_tag="NBH55_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1508261.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            30924..31739
                     /locus_tag="NBH55_000246"
     CDS             30924..31739
                     /locus_tag="NBH55_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCD1508262.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            31890..33056
                     /locus_tag="NBH55_000247"
     CDS             31890..33056
                     /locus_tag="NBH55_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1508263.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            33286..33933
                     /locus_tag="NBH55_000248"
     CDS             33286..33933
                     /locus_tag="NBH55_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD1508264.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            34017..34961
                     /locus_tag="NBH55_000249"
     CDS             34017..34961
                     /locus_tag="NBH55_000249"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCD1508265.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            35096..36547
                     /locus_tag="NBH55_000250"
     CDS             35096..36547
                     /locus_tag="NBH55_000250"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCD1508266.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            36568..37404
                     /locus_tag="NBH55_000251"
     CDS             36568..37404
                     /locus_tag="NBH55_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCD1508267.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            37404..38063
                     /locus_tag="NBH55_000252"
     CDS             37404..38063
                     /locus_tag="NBH55_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD1508268.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            38189..38878
                     /locus_tag="NBH55_000253"
     CDS             38189..38878
                     /locus_tag="NBH55_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508269.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            38884..39840
                     /locus_tag="NBH55_000254"
     CDS             38884..39840
                     /locus_tag="NBH55_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCD1508270.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            39884..40396
                     /locus_tag="NBH55_000255"
     CDS             39884..40396
                     /locus_tag="NBH55_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1508271.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(40652..41200)
                     /locus_tag="NBH55_000256"
     CDS             complement(40652..41200)
                     /locus_tag="NBH55_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1508272.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(41289..42470)
                     /locus_tag="NBH55_000257"
     CDS             complement(41289..42470)
                     /locus_tag="NBH55_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD1508273.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(42706..44214)
                     /locus_tag="NBH55_000258"
     CDS             complement(42706..44214)
                     /locus_tag="NBH55_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCD1508274.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            44373..44564
                     /locus_tag="NBH55_000259"
     CDS             44373..44564
                     /locus_tag="NBH55_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508275.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            44767..45684
                     /locus_tag="NBH55_000260"
     CDS             44767..45684
                     /locus_tag="NBH55_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCD1508276.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            45677..46750
                     /locus_tag="NBH55_000261"
     CDS             45677..46750
                     /locus_tag="NBH55_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508277.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            46898..47416
                     /locus_tag="NBH55_000262"
     CDS             46898..47416
                     /locus_tag="NBH55_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508278.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            47397..47789
                     /locus_tag="NBH55_000263"
     CDS             47397..47789
                     /locus_tag="NBH55_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCD1508279.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            47865..48800
                     /locus_tag="NBH55_000264"
     CDS             47865..48800
                     /locus_tag="NBH55_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCD1508280.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            48933..49712
                     /locus_tag="NBH55_000265"
     CDS             48933..49712
                     /locus_tag="NBH55_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCD1508281.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            49705..50022
                     /locus_tag="NBH55_000266"
     CDS             49705..50022
                     /locus_tag="NBH55_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCD1508282.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            50266..51876
                     /locus_tag="NBH55_000267"
     CDS             50266..51876
                     /locus_tag="NBH55_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD1508283.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            52033..52881
                     /locus_tag="NBH55_000268"
     CDS             52033..52881
                     /locus_tag="NBH55_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCD1508284.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            53112..53591
                     /locus_tag="NBH55_000269"
     CDS             53112..53591
                     /locus_tag="NBH55_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508285.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            53624..55852
                     /locus_tag="NBH55_000270"
     CDS             53624..55852
                     /locus_tag="NBH55_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1508286.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            55981..57624
                     /locus_tag="NBH55_000271"
     CDS             55981..57624
                     /locus_tag="NBH55_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCD1508287.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(57697..58242)
                     /locus_tag="NBH55_000272"
     CDS             complement(57697..58242)
                     /locus_tag="NBH55_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCD1508288.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(58265..58750)
                     /locus_tag="NBH55_000273"
     CDS             complement(58265..58750)
                     /locus_tag="NBH55_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1508289.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(58740..59552)
                     /locus_tag="NBH55_000274"
     CDS             complement(58740..59552)
                     /locus_tag="NBH55_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCD1508290.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(59691..>60802)
                     /locus_tag="NBH55_000275"
     CDS             complement(59691..>60802)
                     /locus_tag="NBH55_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCD1508291.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        20289 a        10881 c        11826 g        17806 t
ORIGIN      
        1 gtattggatt tttttaaaga aaatgattat ttgaaaaata aagaaatcat tatcccagaa
       61 tttgactcgc ggaacatctt gctagcttta gtacgttatg tcaattggga aggctatgaa
      121 aataaaaatc cgtatgtaga agaagcttgt aacgagttga ttgattatgt agagactcat
      181 ttttttaaaa gaagatattc ggaagatgaa aagtccttta tcatacgaga aatagagatc
      241 gcaatcggaa gaaaggcatt tccccttgga ctgaaagaag aagataagaa aagcgcagaa
      301 acgaagccgt tatttcaatt agtctgtcaa ggattggaag agcaaaaagg aattctagac
      361 ttacaagaag aagatgtcta ttatattttt tctttgttca acagtagaaa ttataccaat
      421 gagaacatgg agctgttaag aaaagatttc gaagttgttt ataaaaattt tgttctcaat
      481 gacctatcac tgaatgaatt gattttacag attattagta tcgctgaaat cgacgatgca
      541 gaaaatttta ttttcaagca atccttttta caatttgtcc gtacgttgtg ggcagatgga
      601 caagtatttt taccggaaag gatctatctt ctatcagaaa aagaacaact tctgtacgga
      661 aaaattgtag aaatattgga agagtggaaa aagaaaaatc aaatccaact aagatggaat
      721 gaaaatttca ttcgaaaatt cgtcaaaagt ctttctttga tcaacagtga acaagcgggg
      781 agacaggaaa tagaaatttt tgttgtttca gaaagttcag tcaaacaatt tttttatcga
      841 acccaatttc atttgtatgc ggaagaaggc atcgctgaaa taaatcctat tttatttcgt
      901 tcattagaag aattgcctga tgaatgtcta tgtgcgaaaa aaagagtggt tttgtgcgat
      961 gctgctagct atcaaaatgg attagaaact gaaaagacaa agatctatcc aatctcatta
     1021 aaaggtgcaa acatccattt gtatcatatt gctcaggaac ttcacctatt aaaaaaacat
     1081 atttcttgat gagaccttaa ttaggagacc ttctacaaag ctttttaatt gtctgtagaa
     1141 ggtttttatt tgagtcctga ttcgtgcgaa gatctactta tataacaagc tttctctgaa
     1201 atttttttaa aatgctattg acttagagtc aactctaagt agtagaatag ataaagtgta
     1261 acaaatctat tttcattaaa agaagatagt acggaggaga gaatgtatta tgagtacagt
     1321 attacatgta ttggatgcaa tcaatagccc cgcagatgtc aaaaaattat ctgttgagga
     1381 aatgaaacag ttggcagaag agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg
     1441 tgggcacgtt ggtccgaatc taggtgtagt agaattaacg attgcgtttc attatgtatt
     1501 tgattcacca aaggataaaa tcgtttggga tgtttcccat cagtcttatg gtcataagct
     1561 tttaactggt cgaaaaaatg gttttgaaaa aggacattac cgtgaagtga gcggatattc
     1621 caaccaacat gaaagtaaac atgactattt tacagtgggg cacacatcta catctatcag
     1681 cttagcaaca ggtttagcta aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc
     1741 atttatcggt gacggctccc taagtggtgg cttagctttt gaaggattga acaatggtgg
     1801 tgttttacaa tccaatctaa tcgtattagt aaacgataat gagatgtcta ttgatgagaa
     1861 tcatggcagt ttatataaaa acttagctga gcttcgtcaa accaacggta aaagcagcaa
     1921 taacctattt aaagcaatgg gatttgatta tcgttacgtg gataatggaa acgatctgga
     1981 aacggtgatc cgtttatttg aagaagtaaa agatattgat catccgattg tacttcacgt
     2041 ccatacggaa aaaggtcgcg gttatcagcc ggcaatcgat aataaaatga aatatcattg
     2101 gcatgtacca tttgatctgt ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt
     2161 taatatgatc atcatggatt atttggataa gaaaatgcaa acaagtacag ctcctattgc
     2221 cgcagtcaat gcagcgatac caggcgcctt tggactgaaa acattccaag aaaaacatcc
     2281 agatcattat tttgacgtgg ggatcgctga acagcactct atcactttcg cttctgcttt
     2341 ggcttctcag ggagtacgtc cagtcgtctt ccaatcaagt actttcttgc aacgggcata
     2401 tgatcaatta tcacatgatt tagcgatcaa cgagaatcca gtgattattt tagtcggtgg
     2461 cggaacgatc tcagctagtg accgaacaca ccaaggaaca tttgacattc cttacttatc
     2521 aagtatcccg aacatcaaat atcttgctcc agcaacaaaa gaggatatga tctctatgct
     2581 agattgggcg ttcgaacaaa cagcgcagcc tgtagcaatc cgaattccta cacatggtgt
     2641 agaacatggt acagctatcg caaatgatta tagtattcca gcctatcaaa cagtaaataa
     2701 tggaggaaaa gtagcaattt tagctttagg cggttttctt gaattaggaa aagaaacagt
     2761 aaaagctttt agaaaaaaag gaatcgaagc aacgttgatc aatccaggaa cgatcacaga
     2821 aaatgacgta gaaacattag ataaattaaa agaaacacac gaattagtca tcacattaga
     2881 agatggtagt ttgtcaggag gatttggcga aaatatcagt cgttattatg gtgattcttc
     2941 aatgaacgta ttaaattttg gtgcagagaa agaattcacg gatagcgttc ctgtagggca
     3001 gctttatgaa cgttatcatt taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt
     3061 gaatattcaa taattcatta tgctcaccat ccagataatt gtttatacta gtatctggat
     3121 ggttttttca ttataaaaag caaaggggtg gatgttatgt atcagcaagt agaagaatat
     3181 gtcaaaaagt tccaagaaaa atatcctagt tatcaatcag aaaaattagg tttcttttca
     3241 tttggggata cagaaaaaat ggcagacgag ttgagcgaac tagttgtaaa aaagataaag
     3301 actggtacat caagtggtta cgatctgtat aaacaagaga atgagccttt accaaaagtt
     3361 ggtcaattag acgtggtcct gaatggaaaa aatgaaccag tttgtatcat caaaaatata
     3421 aatgtgacca tcttgccttt tagtgaagtc acgaaagagc atgcttggaa agaaggagaa
     3481 ggggatcgga cattagctta ttggcggaaa gtccatgctg agttttttaa acatgcttat
     3541 aaagaagcgt tgaacataga atttacagaa gagaagctgg tcgtatacga agaatttgaa
     3601 gtaattttct tttagtaaga aaggtctgct gaaagaaata gctcattatt gtctcctgaa
     3661 tagaaaaggg ttacattttt atatgaagct gtatttttat ataacattac tggaggagat
     3721 attgtggata cactggaaca aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa
     3781 aacgagccgc tacacagttc ttttattatg gggagaatgg ctttggctgc aaaagaaggc
     3841 ggagcagttg gtattcgagc aaatacgaca gaagatatca atgaaatcaa gcgaatcgtt
     3901 gatttaccgg taatagggat tatcaaaaga gattacgatg ggtcagaagt gtttattact
     3961 gcgacagaaa aagaagtgga agaacttttg gagacgcaag cagaaatcat tgcactggat
     4021 gcaacttctc gtaaacgtcc gacagaaaaa acaactgctg aacttgtcca aatgattcac
     4081 gaacacggtc ggttagctat ggcagatatt tcgacttttg aggaagcgct tcaagcgcaa
     4141 gaagatggtt ttgatctttt gtctacgact ttagcaggct atacagaata ttcacgaaag
     4201 acgcagacac ctgattttga tttattgaat caaatcatag aacaagtcga tatcccagta
     4261 attatggaag gacatactga ctctcctgaa caagtagaga aagcatatga attaggtgct
     4321 ttttcagtcg tagttggatc tattatcacc cgtccgcaat tgatcactaa aagatatgtc
     4381 gaagccgcaa gtaaagcaaa taactaaaaa acacgcacgg cacagattct tgacaaaaaa
     4441 gaaaaattga tagatagttt aaagcagatt ctaagaagat gaaaaaagca ttacttttca
     4501 ttttgtcttc aagatctgct ttttaacgaa ttttatagaa agaaggcgaa accatgtttg
     4561 aacggagtcc ttcagaattg acagaacgtg aaaattacaa gttgctgatt ggttcagtca
     4621 tccctagacc agtagcagta gtaacgacta aatcagcaaa agatatagtg aatatcgcac
     4681 ccttcagtta cttcaatatc gtcagttcga atcgacccat cttatccttg gcgatccaga
     4741 gaaaacaaga tgcagtaaaa gacacagcaa agaatattct tgagacaaga gaagctgtta
     4801 tccatatcct tgatgaggac aacgtagaag aagtaaataa aacagcagcc aatctgccta
     4861 atgaggaaag cgaactgtac ctgactgact tgactttaac tgattcagta atcatttcag
     4921 tactagcatt gacagaagca aaagttcgtt ttgaaacatc cttatatgaa catgtcgaga
     4981 tacggaataa aaataaagta acagctgatt tatttttgct agaagtacag aaatatcatt
     5041 taaatgaaac agtctatgat gataagacag gaaggatcga tccaagaaaa ttgaatgctg
     5101 taagccgttt ggcaggaaat gattatgcag caataggaga aattttcacg attgcacgtc
     5161 ccgaataaaa aggagcacta gttatgttaa aaataagcta taaaccatcg acagattcaa
     5221 aagaaatgaa aaaagaatat gaaacagtga atgattttct gcaagggcag tacttagaag
     5281 ttccgccatt gcaagaccat tttgtcgtta ctactgttac tttagatggt aaggaaatag
     5341 aaatgccaga tcaaacgatt agcggattgt ttaattactt taacaaataa atatttattc
     5401 cgaaaagtga aaaaacatat aaaaaaacac ataaaactaa aaaattatca aagaatcctg
     5461 gtgcaactga aagaatttgc aacgggattc tttcaactta tttgttattt ttttctaaaa
     5521 aatattaaag attgttaaat ttcactattt catttcaaat tccattgcct aatcattagg
     5581 aaaagtctat catgaactcg taaatagaat atagtaataa ccttgttagg tgaggctcct
     5641 atacaaacat aggctgttgc ccagaaacgt cgaaagtcgc caatgggtaa aacagaaact
     5701 gttggattaa ggctttttcc aatagctaag aatgttttct ttacgttgta tagtgctaaa
     5761 actcggacga tgagatgatg ctaggtcaga attcttgttc gaaaaggttt ctgacctttt
     5821 ctttatattt ttgtgactga cttatttgat gagacaaact cgctcctcac ctgacaagtg
     5881 gattgctgtg tgaggaggag atgcggtgaa aaaagatcga ttggtggatt ttgggtcctt
     5941 atttgacttt gtccatgatc ggtcttttag aagtatacag tgccagttct tatcgtttat
     6001 tgcaagccga tgaaaataca aaaagtctat tattgcgtca actaattttc atatttttga
     6061 gttggagcgt gatcttctta gctcgttcag tcaaactaca ctatttactt caccctaaaa
     6121 tagcaggata cggtttagcc ttatcgattt tctttttagt attagtaaga atagggatat
     6181 tcggtgtcac tgtcaacggc gcacaacgtt ggatctctct gtttggcatt caattccagc
     6241 cttctgaact ggcaaatctt tttttgattt tttatttaag ctggtttttt cgtgacggaa
     6301 atagtagccc aaaagatcta aaaaaaccat tcctgattac agtaggtata acttttctga
     6361 ttttatttca gccaaagatt gctggagcat tgatgatcct ttcgattgcg tgggtcatat
     6421 tttgggcagc ggcggttcca tttaaaaaag ggatctatct aatcgttact ttttctgcat
     6481 tgctgattgg agcagcaggc ggggtattat atttaggaaa taaaggttgg cttccacaga
     6541 tgtttaatca tgcttatgaa aggatcgcga ctttaagaga ttcttttatc gatagtcatg
     6601 gtgcgggcta tcaaatgacc cattcgtttt atgcactata taatggtggc atatttgggc
     6661 ggggattagg aaatagtatc acaaaaaaag ggtatcttcc agagacagaa acagatttta
     6721 ttttttcgat tattacagaa gagcttgggc taatcggtgc attgtgtgtg cttttcttat
     6781 tgttctcatt atgcatgaga atcttttgtt taagcagtcg ctgtaaaaat cagcaagctg
     6841 gattgtttct tttaggattc ggaactttgc tattcgttca gacgataatg aacgtgggaa
     6901 gtatcgcggg gctgatgcca atgactggtg tgccattgcc atttgttagc tacggaggaa
     6961 caagttatct gatcctttct ttaggaatcg gaatcacgtt gaatatttct tcaaagatac
     7021 aggccgaaga gttggcgctc tatcgacctg aaaaacaata aagagaaact gagaaactct
     7081 cagtttgaat tatcataaag tacaatcttt tatttttcaa gcgagaggtt cttttagaaa
     7141 gaggaattcg caatgaaagt atctcaaaaa atcatattat ctattttgtt actgcttaca
     7201 ttagtagttg gctatttttc gattgagttt atccatggat tttcgtctgc aaaacagagt
     7261 tcaactgtga aaaaggtaga gccgaaaagt atccctacca cactaaatgt ggctttgatt
     7321 ggttcagatg ctcgttcgaa agacgaaaac ggtcgctcgg attcacttct ggttgcacaa
     7381 tacaaccaga aaacacaaca agcaaaactg atctctatca tgagagattc atatgtcgat
     7441 attccaggtt acggaatgaa taagataaat gcagcttact cttatggggg agttgattta
     7501 ttaaatcaaa cattaaagga aaatttcaaa tttgaagctc cctattatgc aagtatcaca
     7561 tttcaagatt ttatcgattg cgttaatgaa ctgtttcctg gtggagtcaa gatcgatgca
     7621 gaaaaatctc tagatttaga tggcgtatat ataaacaaag gagagcaagt gatggatggt
     7681 aatactttac tccagtatgc tcgatttcgg gaagacaaag aaggagactt cgggaggatc
     7741 agaaggcagc agcaagtgat caaagctatc tctcagcagc tgaaagacgt aacttcaata
     7801 ttcaagctac ccaaagcggt tgggaaatta cttggtagta tccaaacgaa ccttccagaa
     7861 agtgtgcttc tggattgtgg tatggatttc ctcaaagaca tatgttagaa taaacaggta
     7921 taaatagtga aataaaggaa tgacaagcaa gagaaggagg aaaaaatgaa aagaagtgac
     7981 aagcacggca aaaatcgaac aggcgcttat attgccggcg cagtgatctt aatagcagct
     8041 gcgggtggcg gttattttta ctaccagcac tatcaagaaa cccaagcagt agaagctgga
     8101 gaaaagacag ttgagcaatt tgtccaagct ttgaacaagg gagattataa caaagctgca
     8161 gaaatgacat caaaaaaggc ggcaaataaa agtgcattat ctgaaaaaga gatcttagat
     8221 aaataccaga atatatacgg tgctgctgat gtcaaaggac ttcagatatc gaatctaaaa
     8281 gtagataaaa aagacgattc tacttacagt ttttcatata aagcaaagat gaatacctca
     8341 ttaggtgaat tgaaagatct ttcttataaa ggaacattag acagaaatga tggtcaaacc
     8401 acgatcaact ggcaacccaa cttggttttt ccagaaatgg aaggaaatga caaagtaagc
     8461 ctgaccacac aagaagcagc aagagggaat attatagatc gaaatggtga accattagca
     8521 acaacaggca aactaaaaca attaggagtc gttccaagta aacttggaga tggaggcgaa
     8581 aaaacagcca atatcaaagc gattgcttcc tccttcgact taacagaaga tgctatcaat
     8641 caggcgattt cacaaagctg ggtacaaccc gattactttg tcccattgaa aatcattgat
     8701 ggagcaacgc cagaacttcc agctggagct accattcaag aagtagacgg cagatattat
     8761 cctttgggtg aagcagctgc tcagctgatt ggttacgtgg gagatatcac agcagaagat
     8821 attgataaga atccagaatt aagcagtaat ggaaaaatcg gtcgctcagg tttggagatg
     8881 gcttttgata aggatcttcg tgggacaaca ggtgggaaat taagcattac agatgcagac
     8941 ggtgtagaga aaaaagttct gatcgaacat gaagttcaaa atggcaaaga tatcaaattg
     9001 acaatcgatg cgaaggcaca aaaaacagct tttgacagtc taggaggaaa agctggatca
     9061 actgttgcga caacgccaaa aaccggtgat cttcttgcgc ttgctagctc tccaagctat
     9121 gatccaaaca aaatgacaaa cgggatctca caagaagatt acaaagctta tgaagaaaat
     9181 cctgaacatc cattcatcag ccgatttgcg acaggttatg ctcctggatc tacgtttaaa
     9241 atgattacag cagcaatcgg tctcgacaac ggcactatcg atccaaatga agtgttgacg
     9301 atcaacgggc ttaaatggca aaaagacagt tcttggggat cttatcaagc aacgcgtgtc
     9361 agtagtgatg tatcacaagt agacttaaaa actgctttga tctattccga taatatatat
     9421 gcggcacaag aaacgttgaa aatgggtgag aaaaaatttc gtacaggctt agataaattc
     9481 atttttggtg aagaccttga tttgccaatt agtatgaatc cagcacaaat ttctaatgaa
     9541 gatagcttta actcagatat cttgctagct gatactggat atggacaggg cgaacttcta
     9601 attactccta tccagcaagc agcaatgtat tctgttttta ccaacaatgg cacacttgtc
     9661 tatcctaaat tgattgcaga taaagagaca aaagataaga agaatgtcat cagcgaaaca
     9721 gcagtacaaa cgatcgtgcc agatctgaga gaagttgtgc aagatgtaaa tggtacagca
     9781 cattctcttt ctgctttagg gattccgttg gcagcgaaaa ctggtacagc ggaaatcaaa
     9841 gaaaaacagg atgtaaaggg gcaagagaac agtttcttgt ttgctttcaa tcttgataac
     9901 caaggttata tgatggtcag catgttggaa aataaagaag atgatgattc agcaaccaaa
     9961 cgagcatcgg aactattgct atacctcaac caaaattatc aataaaaaat tgctttttaa
    10021 gtacagtaaa aggactgtgg cgttatcgtc acagtccttt tttcaaataa acggttttca
    10081 cgatgtctct ttcacaactt ctttttgatt tactcacact tcataaactt tttttgtaaa
    10141 atatttgaga acaagtggag aaataagtgt acttaataaa atcacgataa ctaaaggtga
    10201 atagtactgt ggttcgatca attgactttg ctgtccaatt tgtaaaatga tcaaagccat
    10261 ttctccacgc gaaatcatac cagcaccgac catcaatgaa ctatgttgag aaaaaccggc
    10321 aatctgagca ccaaaataac ctccaagcaa tttggtcaaa atcgctacaa gcgtcaatat
    10381 agaaataaag agtagttgtt cactaaagtc agaaaaatca acttctaatc ctacactgac
    10441 aaaaaatact ggtataaata ctgcatatcc taaagcctct acattgtggt atacttcttc
    10501 tttaaccttt gtctgactga cagctatccc agcaaaaaaa gcaccaatga cagagctaag
    10561 cccaacaaga tccgctacgt aagacattcc taaacaaata actagcgata cgatgataat
    10621 agaagaattt gcgtacactt tttctgcaaa tgaaagtagg aaaggcgcaa tccatttgac
    10681 taataagaaa ataaaaacaa aataaattag ctgttcaatc aaaacaagcg gtaaagaagc
    10741 attgctagaa gcttcccccg ttagaaaaga taggctgaaa cttagaacta aaacgactaa
    10801 aatgtcatct accacagaag cacccaaaat tgttgatcct tcttttgtat tcactacgtt
    10861 tagttctttt aatacttcta ctgaaatact gacagaagtt gccgcaagaa taatcccaaa
    10921 gaaaatcgct tcatttcccg aaacttggaa tgctttgcct gtcaaccagc caaaaacaac
    10981 cgggaagatg atcccaagca aagcaacgaa cataccaggt cggaaatatt ttttcaaaag
    11041 agataaatca ctttccagac cagctaaaaa cattaataag atcacaccaa tctccgaaaa
    11101 gtcatggact agaatatcag gtttgaccca attcaatcca gcttggccta gcaaaacacc
    11161 aacaagtaat tgtccaatca ctgctgggat acctaatctc cgcgagatat gtgagccaat
    11221 cgttgttgaa atcaatatca ggcataaaat accaataaat tccattctca tcattccttt
    11281 gtataaaata tagcccgaac ttagcataac atacattatt gaaaaaatag gaaaatatac
    11341 tcacaaaaaa accaacaatc caaaagattt ttttggattg ttggttttta tacggtctat
    11401 tttaataact aaaaatgatt aacgtatagt catttttctt attctgatga aattgataat
    11461 ctcctggatg cccatgaaaa tcaaaataaa gccgaatatt tggaatagaa gcatcaaact
    11521 tttgaatgga ttgaacaata agacgaatcc gccaatcaac aacagaatac tactataaac
    11581 actccaactt ttagcttttg tattcatgct gaagaacaac tggaacaagc tgttgatgat
    11641 aacagctaat ccaagaagaa tcggcaggat agaaacgatt gcaggtgcaa aagcatagac
    11701 gatcagagct aagatcagga aaaaaagacc actgaacaga ccgatactcc aagaacctgt
    11761 tttcctttta aaacggtaag cctcaaaaag attcaacaac ccaagcaaaa caagataggc
    11821 ggtgatcaga taaccgataa aatgaaaaac agcagaaggg tttagcacaa tcgcaatccc
    11881 agaaataata taaacagcag ctcttaataa ggcgtatttt tgaatatttt gtttaatcgt
    11941 ctcaaacata taagaaaacc tcctttgact tattttaacg tgaactagtc attttaccaa
    12001 attaaatgtt acaaaatgag cgaaggagaa gaatatctct ggaaaaatca acttctgagg
    12061 atcttttcca tttttttgcc ttttgctagc tcatcgatca ttttatctaa ataacgaatt
    12121 tctttcatca agttatcctc gatgtcttct actcgtacac cacaaacaac acctgtaatc
    12181 aaggaacgtg aaggattggg gacaggggct tccgcaaaaa attgttcgaa agtcacttgt
    12241 ttttgtagtt gatattctag ttctgtttgg gaataacccg tcagccaaca aatgatttca
    12301 tctacttcct cttttatacg atcttttctc tccgcttttg ctacatacat aggataaacg
    12361 cttgcaaaac ttgtcgtaaa aatacgattt tgcgccataa tcagtcacct ttcttttttt
    12421 caaattattt actacttata gtatataaga aaaagagaag aaaaagcgat tattcaaaaa
    12481 aacaaaaagg attgtgacaa aattcttgtc accatccttt tttccgctct ctgacttttt
    12541 acaatcttca tagctcaagt gcgcatcaga taatcgaatg ctcctaaagc tgcagtagct
    12601 cctgaaccca ttgaaacaat gatttgtttg tatggactat ctgtacaatc accagctgcg
    12661 aaaacgccgg ggatattcgt tgcgccattt ttctcggtca ctatttcatt tcttggattc
    12721 atttcgatcg ttccatctag ccaatccgta ttagggagta agccgactag aatgaatact
    12781 cctgaaacag ctagtgtatg catctccatc gtacggcgat cgatatagga gagagattct
    12841 acctgattcg taccgctgat ttctttcgtc gctgcatgtt tgataacagt gacattcgca
    12901 agagagacta gtttatcctg taagatttga tctgctttta attctggtaa gaattctaga
    12961 acatatacat gttttgctaa cccagccaaa tcgattgctg cttcaacacc agaattacca
    13021 ccaccgataa cagcaatctc ttttccttta aataaagggc cgtcacagtg tgggcaataa
    13081 gcaattcctt tatttttaaa ctctttttcg cctggtacat tgatagaacg ccagtgcgca
    13141 cctgtcgcga tgatggcggt ttttgcttta agtgatatac cgttagctaa gccaatatta
    13201 ataaaatgtt gctctttatt taaggaaacg gcctgttgat tcatcataat gtcgataggg
    13261 taactacgca catgactttc tacttgctgc atcaattttg gaccttctgt gtaagaggta
    13321 ccaatcatat tttcaatccc tagtgtttca acgacttgtc cgccgaaaga atcagcgaca
    13381 agtcctgtac ggattccttt acgtgctgca tagatagctg cactactagc tgctggtcca
    13441 ccgccaatca ctaatacatc ataaggttct ttttcaaaca actcttcttc aatcaaaggg
    13501 ccagtgattt tttcaacaat ttgttcgatc gtcatgcgtc cgctactaaa ttcttctcca
    13561 tttaaaaaca cagttggcac tgccatgatt tttttatcat cgatttcttt ttggaacatc
    13621 ccaccttcaa tcatcgtatg agtaatattt ggatttaata cagctaatat attcaatgcc
    13681 tgtacaacgt cggggcaatt atgacaagtc aagctaacat aactgtcaaa atgcaatgtt
    13741 tgtgttattt ttttgatttt aagactcaaa gattcactga ttttcggtgc ccgtccacta
    13801 atttgtaaca tagccagtaa aaaagaggaa aattcatgac cgagggggat accagcaaaa
    13861 acgattccac tttctttgcc aactggattg atagaaaaac taggtgtgcg tatcgaatct
    13921 cttttttccc aagagatttt aggagacatt tctgctattt ccttcgtaaa ttcagctagt
    13981 ttttgactat tttcactatt atccaaacta acagaaaaaa caataggaga ttcaatcagt
    14041 gctaaatatt gttcgagatt ttgtttagtt tctttgtcta acatatccac cacctaaatc
    14101 tttccaacaa gatcaaaact tggtttcaat gtttcgccac tttctttcca ttttgccgga
    14161 caaacttcac ctgggtgggt acgtacatat tgagcagcgc gaatcttatc aatcagtgca
    14221 ctagcgtcac gaccgattcc atctgcattg atttccatag cttgaacgat tccgtctgga
    14281 tcaataataa atgttcctcg ctgagctaat ccttgctctt catccaaaac atcaaaaata
    14341 cgagaaatct gatgagacgg atcaccgatc atcacgtatt cgattttacc gatagcatct
    14401 gatgtatcat gccatgcttt gtgagtaaag tgggtatcgg tagagcaaga aaagacttca
    14461 acacctaaag atttcaatgt actgtattgt tcttgtaagt cttctaattc agtaggacaa
    14521 acaaaagtaa aatcagctgg atagaagcaa agaatactcc agttccccat aagatctttt
    14581 tcactgactt ctaaaaattc tccttgatga taagcatgtg cattaaattc ttcgattttt
    14641 ttcccaatca atgacatagt aaaacctcca ataataatta tttatttata attattgtaa
    14701 tttagttgga gttgcaaagt caatggatat tttgttattt aataaagatt actcaaaaac
    14761 gaaggttaat tataaaaata gaagaggtcg caagaaaatg ctactgctgg aaatactcgg
    14821 gataacgttg agtaacagtt tcctgttctt tcagcatcat gtgtaagtgc aatcccgttt
    14881 gaaatcgagc ttcttctggt tttttatcaa ctaaagcctt gagtaaatca taatgttgtt
    14941 ccatgatcgt ttcccatgga agcccctgaa tggtcactcg taaccagcgg aaacgttcta
    15001 aatgggtatt atggtcatct aaccagttcc aaacttcttt tcttcctgca atttcgaaac
    15061 aagcttcatg aaataaatta tctgcacgga aaaaagcgcg ttcgtcattt attttgactg
    15121 ctttttcttg ttcttctaaa atcgtttcta gcactattcg tgtttgtgtg tccaatttgg
    15181 aacaacattc aatcatgatt tgctcttcca actgttctcg gacaaagcga gcattttctg
    15241 cagaaacaag attaattttg gaaacatacg taccactttg aggtatcgta tacacgagtt
    15301 cttgctgacg tagttgaatc aatgcttcgc gaatgggagt ccggccgata gcaagatgtt
    15361 cctccagtgc cttttcagat attttccttc caggttctaa gtcagcatag ataatttgtt
    15421 tacgaatcgc ttgataagct tgtgattgca agctgctttt catctttatt cctccaaaat
    15481 catttctatg aacgtagtat aacagaagag gtctgaaaaa caatctggta tgtcacatta
    15541 actagtaaat ttgtataaat agtaaaattt tggttgacat agcgattttt attgcataaa
    15601 attcacttga atacaaaaac taatatgtta gtttaggagg ggaaaaatga ttgatttaaa
    15661 caactggagg aactttatcc ctgaattatc tcaaaaagaa gtagaaatac ctcattttga
    15721 ccgtgcatca ttaagagaaa atggacaaaa aaatcctgtc tggatccatt tcggtggcgg
    15781 aaatctttat cgaggatttc atgcagaaat cgctcaacgt ttagcggatc taggcgaatt
    15841 gtccactggt gtcattgttt gtgaaacatt cgatgaagaa ataattgaaa gggcttacca
    15901 agattttgat gatttgattc ttgaagtggt catgccaagc agtggagagt tcgtcaaacg
    15961 tatattagct gcgacagctg acagtctgta ttgtcatcca gattgttctg aacactttga
    16021 taagatgatt cgacactttg aaaatcctag tttacaatta gttacttttg ctattactga
    16081 aaaaggttat cgattgaaag atacaaataa taaatatata ggaccgattg cagtcgatat
    16141 aaaaaatggt ccaaagaagc catcacatac aatgagtatt cttgctgtgc tactttataa
    16201 acgtttttta tctggagctt ggccgattgc acttgtctct acagataatt tttcacaaaa
    16261 cggtagtcaa ttacgtactt ctttgctaac gatcgcaaaa gaatggcaaa aggaaggtta
    16321 tgtacaaaat acatttatcc aatacttatc gaatgaagaa attgttgcgt ttccatggac
    16381 gatgattgat cgtatcaccc cgaatccttc cttgtcagtt agcaaaatgt tggcaaaaga
    16441 gggcttttca aatatgtcga ttttccaaac cgcaaaagga aataatattg ctccatttgt
    16501 caatacagag gacatccatt atttaatcat ggaagatcat tttcccaatg gacgtccgaa
    16561 attcgaaaaa gctggggtat tcttgactga tcgtgcaacg gtagataaag cagatacgat
    16621 gaaggtgacg acttgcctta atcctttgca tacgtcttta gctattatgg gttgtttact
    16681 ggggtatagg agcatagctg aggagatgaa agatccagat ttatcgctgc tagtttctga
    16741 aatcgggtac aatgaaggcc tcccagttgt ggagtctcca ggtatcattg atccaaagat
    16801 ctttctggaa gaagtaatta ctgtgcgctt ttctaatcca aatattccta atatgcctca
    16861 gcgaatcgcc acggatacct ctcaaaaggt ggcgattcgt tatggtgaga cgattaaaaa
    16921 atatatggaa atagaagtta aagacccgca gacacttgta ttcataccat tgactctcgc
    16981 tgcttggctt cgatacctta ttggcattga tgatcaagga aaaccattca tcccgagtcc
    17041 agacccacta ttgacagacc ttcagcataa gctttccgat attcaactgg gtacgcaaga
    17101 tacatcacat attcatgaga aaatggctcc tattttgtca aataaagaaa tttttggtgt
    17161 agatctttac caagcagagc ttggcagaaa aatagaagaa atgttcatca agatggtagc
    17221 aggaccaaat gccgtacgaa aaacattaca ggattactta caagaacatg gaggaaattt
    17281 atcatggaaa tgacttttag atggtacgga tcacaggaag aaaagattac cttagatgaa
    17341 atcagacaga ttcctgcggt caaagggatt gtaggcacat tgatggatat accagtagga
    17401 gaggtatggc ctagggagag gatcaaagaa ctaaaagaag aaattgagtc tcgtgggtta
    17461 acactaaaag taattgaaag cgtcaatgtg catgatgcca ttaaaattgg attaccttcc
    17521 cgtgagcagt atatcgaaaa ctataagcag acaatccgaa acttggcaga atatggaata
    17581 gaagtcatct gctataattt tatgcctgta tttgactggg tgaaatctga ccttgactac
    17641 cgcttagaag acggctcatc cacgttagct tttatatctg atgatattcc agatgatcca
    17701 agagaaatcg tcgcgcgcat cgaacattcc agcaatgaat ttggattgcc gggatgggaa
    17761 cctgagcgct tagctcctat caaatccttg cttgaagcct atgcatcagt agacgaagaa
    17821 aaacttcgag aaaattttgc ctattttctc caatcaatca tcccaacatg tgaagaggta
    17881 ggggtcaaaa tggctgtaca tccagatgat ccgccatact cgatttttgg attagctcga
    17941 atcgtaaaga atcgagaaga tttagattgg ttgtgtcgca tagtagacag cccttataac
    18001 ggaatcactc tttgtacagg gagtattgca gaagatccag aaaatgatgt atatgggatt
    18061 ttagcagaat tttgccaaag agatcgaatc ccatttgcac atgttcgaaa tattaaattt
    18121 ttagctaatc gagatttcta tgaggcacca cataaatccg aatacggttc attagacatg
    18181 taccgcatac taaaaacaat gtatgacaac ggatttaacg gatacatccg tcctgatcat
    18241 ggaagaatga tctggaatga gacaggaaga ccaggatacg gcttatatga tcgagcatta
    18301 ggtgctgttt atctaaatgg attatgggaa gcaatcgaaa aaggaaacaa aagtaaagat
    18361 aattagtgaa aaaaactgta attttattat gtaaataaga aggatgttca aattcttgat
    18421 acataaatcc ggaggttgtt aataagcatt cggatttatg tatcttattt ggtctaggaa
    18481 gtaacttgat tttctttttt cgattgatcc atgagtgctt cttcttttaa ttcaaagata
    18541 tattctgaga tcctcataat cacttttttc cagtcttctg gtgtgatgat tccttttaaa
    18601 atatagattt ttttgatatg agaaggcaat tgtttcaaag ggcagtcgct gataacgatg
    18661 ttgcagttct ccataaaatc attttgtatt ttgatcgatt catttgaaaa aatttgttct
    18721 atcttaacga tcagatcttt actgatataa aaatcacgcg tatagttcac accaatcaca
    18781 attcgcggct gttttttgta gcgatcatac aaatgtacta ggtcttcaac catccattgc
    18841 acattttcct tttctaaaat aactggtaac aagtgttgat tttgttctct gaaatccata
    18901 taaaattgtt caatggcggt ttggacttct ttcaatacgc tatgttcttg ataaagcatg
    18961 aaagtaggaa ggggcgtatt tggttgattg aagttaagaa atccattata aagtttattt
    19021 cgtgcgaatg ccaatgagta aagaatcttt tctttttcag gtataaacaa atcaaataca
    19081 gtacaaaggg actccaccat ttctcttgaa taaagaacat gaggaacgtt tgcttttttt
    19141 aaaagtttgt attgctccaa gcttgtttct tccgattcga ttttcgtgat agaaagtcga
    19201 gctaaaatat tcactaagat gcgttgttcg cgacaaattg gcagattagg tcgcaaaata
    19261 tctacagaga gatcaataaa aaagtcaata tattcatact ctgtgaattc tttttgtacg
    19321 tctttcacag aagttgcagg aaaattctct ttacatattt ttaatagaaa atataattta
    19381 tctatcatac cgctatttag tcggtcaagt acttcttttt taaaaaaagt attcatcata
    19441 aaagccacta cttgcggatc ttcatcataa aattctgtag cagtacttga aaagatgttc
    19501 caataaatat ctaatgctgc atattgaatt tgtacctctt ttccagaaaa agactttctg
    19561 ccattattag aaaaggagat agacaaaccg tactatgcta aaaatgcatt aatctttttc
    19621 attttcgtat acaaataaga ggcactatag tttgtttgct gactgaaact gataatcgaa
    19681 tttgtctgac agctgaacaa agaagtcaag atctgataac tgactgattc gttgcagtat
    19741 cttccaaata gacgataata gcagtaagcc ggatcaattc cttcaaaatt caactttaaa
    19801 aaaggttttt gcttatcaat atgcagttct gttagattga gacgctcaaa gtcttgttct
    19861 aattctttga tgtatagtaa aaaatttgat tcagacatat ggaaatgatc tgctaaatat
    19921 tgtgtagtaa taaagaaacg atgttcagat aggaaaagaa aaatatctaa ttttgtattg
    19981 ctttttttct tcaagataaa cgttttaatc gcttcttgag tttgcatgaa agaggctcct
    20041 ttttacttaa attgttaaaa cttgaaaata ttactgtttt tttcataaat ttagacaaaa
    20101 aaacagtaaa catataggta gaatctctac tatcttattt cttgcataca aaaaaatcaa
    20161 ggagtgagag agttatgaga tattcaacca tcttaatatc aacatttgca gccttaagct
    20221 tatcgcttac tataaatcag acagtccaca cagaggagac agaaacagcc aaatggacag
    20281 aaaccaagtg ggccaacgtc aatcgaatta cctttcagga tgaattttca tccttcaatt
    20341 caggagtcgc aaaattaaga aaaggacaaa cagcgaccat caaagcaaaa attgactatt
    20401 cgggagactt agcacctata ctacaatcag ctgttacatt tgagaatgtt gatcccactt
    20461 tgtcgattgg ctatgacgag aacactttga ctatccaaca caagcaagcg atatttacct
    20521 tcaccgttac attaaatcag gacttgttaa aaccagcgct ttttactgtc aaggtatcag
    20581 atggactacc gaacgatcat catttaacgc cgtatagtca gcggcagaca attgagcaag
    20641 attcagccat cgactcagga ggagatcttg ttgaagaacc aacagataaa ccagaaaatg
    20701 aaaataagcc agaagtaccg cctacagaaa atccagacgg agaacagaaa ccggaaatag
    20761 aacctggaga agaacccgat acagaaactc aaccagagcc agacaataaa tcaaaacctg
    20821 aaataacccc tggagaaaaa ccagatgtaa ctccagaacc agatacggat tctggaaacc
    20881 aaacagtacc ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag
    20941 aggtagatcc agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc
    21001 aaggaatacc agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta
    21061 aaaaatcaaa tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa
    21121 tatttgatag cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgctg
    21181 tcctgaaacg aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact
    21241 gtggagcgga atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca
    21301 aaacagatct ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa
    21361 tatccagaaa atgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact
    21421 ttatctttct tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat
    21481 ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc
    21541 ccaaacgcca aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag
    21601 ttctgttttg ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat
    21661 caaaatcgta atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa
    21721 agatagagga atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat
    21781 catgatcgct aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag
    21841 caacgtattt aaaagagcga caacaaattg agcttctaat accaaaccaa atgtattgac
    21901 aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa
    21961 caatcttgag aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa
    22021 tgacatagca aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga
    22081 agcgccattt tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg
    22141 taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa ttaataacgg actggatcat
    22201 gtcaaatgtt tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta
    22261 aacgatcagt gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg
    22321 aatgatttga acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt
    22381 aatcatactt ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg
    22441 tattttttca ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca
    22501 aaaaataaac acgttattca ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa
    22561 aaaccgctaa atgacgttat cttatgagtt ggttttcctc ataaaatcgt tcatctagca
    22621 gtttcagccc tttttctggt cttgatgaaa attatctagc atttttcggg tcagatgttg
    22681 cagtgtatcc aattcttcct gttcaagtgg aagataagac ttgatctttt gtggaatacc
    22741 ttctgcttgt ttttgcaacg cagcaccttg agcagtcagc gtaacattta cgattctttc
    22801 atcacccgtt tcacgctgac gattaatata atttttttct gctaactttt ttagtaaggg
    22861 agtcagcgta ccagaatcca gacacaaatc atttcctagc tctttcactg aagtcttttt
    22921 cttttcccat aaaactaaca tcacaagata ctgggtatag gtaagattca gcggcttgag
    22981 gaggggagtg tagtgtctaa tgatttcttt agaggcggca tataaaggaa aacatacttg
    23041 attaactaaa tatagatctt ccatttttcc acctccgctg ttttactata gtgtatcata
    23101 gcggatttac acaaaaaagt ctattcacta actgctttca gagtaacttc tatatttcca
    23161 gctgtagctt ttgaatacgg acacacttta tgcgctgcct ctaataattc ttgcgtttct
    23221 tccagagaaa gaccttcgat agatccttca atctctacac ctaagacaaa gctcgtttca
    23281 ctttctgaat aaagggaaac ttgtgcactg acagtagaag ccccagagat atttttttgc
    23341 tgtttgatca attctaatgc actgttaaaa catgcactga aaccagctgc aaacaattgt
    23401 tcagggttgg ttgcattttc gacgcgttta ccaggagctg caatattcaa ttcgaatgta
    23461 cgatcgggat tatgcacttc tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac
    23521 atttttttca taaaattacc acctttaatt atattttata caattgaatt ttacacaata
    23581 taattgtgga aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac
    23641 agacgcgctt tctcgaatag caaacatata caaaaaatat cagagacaaa aaataatctc
    23701 atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa attacttcac
    23761 gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca
    23821 caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt
    23881 tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt
    23941 cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtac
    24001 catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt
    24061 gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa
    24121 aatcgatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact
    24181 agattctttt attataccag ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac
    24241 caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga
    24301 caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat
    24361 gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct
    24421 ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat
    24481 aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg atataagtca
    24541 tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa
    24601 taaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca
    24661 agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt
    24721 ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca
    24781 accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaccaagc
    24841 ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca
    24901 taacgatatt caacactaaa ataactatag ctcggtcaaa gcctaaagca gcttccagta
    24961 atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga
    25021 tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc
    25081 tcgtttcttt catttattca cttccttaat tcattaataa tactcttatc ttattataac
    25141 ttacttttca ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta
    25201 tgtttcattc ttctagaata tacttctacg ccaaaaactg ttaaaaaact tttactttag
    25261 aaaattggag aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag
    25321 actaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg
    25381 gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac
    25441 ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt
    25501 atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc
    25561 ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca
    25621 taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg
    25681 ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc
    25741 atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc
    25801 gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg
    25861 atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa
    25921 gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag
    25981 atccagaccg agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt
    26041 acttgacgga ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca
    26101 atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac
    26161 ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg
    26221 tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa
    26281 aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga
    26341 ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca
    26401 tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt
    26461 attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc
    26521 atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc
    26581 ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga
    26641 tgaattatgc atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt
    26701 cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag
    26761 gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta
    26821 acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta
    26881 tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta
    26941 tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt
    27001 ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa
    27061 atgatcgtga agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt
    27121 actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc
    27181 aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca
    27241 tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc
    27301 attataataa gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag
    27361 aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc
    27421 ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt
    27481 ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga
    27541 aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc
    27601 gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg
    27661 tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga
    27721 tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat
    27781 cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa
    27841 agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa
    27901 tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta
    27961 cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc
    28021 aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg
    28081 aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac
    28141 tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta
    28201 tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg
    28261 aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga
    28321 ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc
    28381 aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat
    28441 tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac
    28501 aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc
    28561 agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata
    28621 accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat
    28681 ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt
    28741 agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg
    28801 ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc
    28861 gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca
    28921 ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg
    28981 attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat
    29041 cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg
    29101 atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc
    29161 gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca
    29221 tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct
    29281 aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt
    29341 tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc
    29401 acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc
    29461 ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac
    29521 gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta
    29581 gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta
    29641 gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg
    29701 atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt
    29761 tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat
    29821 acggggaatt tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc
    29881 gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa acttacgact
    29941 ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt
    30001 atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa
    30061 tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact
    30121 tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg
    30181 ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag
    30241 gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga
    30301 tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt
    30361 atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga
    30421 tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc
    30481 tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt
    30541 ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg
    30601 atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc
    30661 tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt
    30721 tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg
    30781 atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga
    30841 tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa
    30901 caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac
    30961 gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga
    31021 tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga
    31081 tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa
    31141 cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga
    31201 tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc
    31261 atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg
    31321 gtatacgccg ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc
    31381 ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca
    31441 ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat
    31501 tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat
    31561 tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag
    31621 ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg
    31681 tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac
    31741 aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg
    31801 aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta
    31861 atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat
    31921 tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat
    31981 acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga
    32041 aagagattac aagtaacgca tctttggcac taggagaagc ctatatggat catcgaatcg
    32101 agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt
    32161 tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa
    32221 acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag
    32281 atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag
    32341 cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt
    32401 tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg
    32461 tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa
    32521 aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag
    32581 gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc
    32641 ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc
    32701 atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc
    32761 ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc
    32821 aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa
    32881 agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa
    32941 tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga
    33001 ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga
    33061 ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag
    33121 tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat
    33181 tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt
    33241 atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt
    33301 atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa
    33361 ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc
    33421 tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact
    33481 cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca
    33541 gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata
    33601 gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg
    33661 gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa
    33721 gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac
    33781 aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg tactacttat
    33841 tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca
    33901 ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat
    33961 ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga
    34021 gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg
    34081 tccactgcat gactacccag gaattagatg aactggctca ttcagaagca ggagcattta
    34141 tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg
    34201 ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag
    34261 agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca
    34321 ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt
    34381 ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag
    34441 cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc
    34501 cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag atggctgata
    34561 tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt
    34621 atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg
    34681 gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga
    34741 aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac
    34801 atctgctatg tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg
    34861 aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt
    34921 caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg
    34981 attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact
    35041 cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa
    35101 tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt
    35161 tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct
    35221 tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt
    35281 ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac
    35341 ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca
    35401 agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa
    35461 aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac
    35521 accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc
    35581 tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg
    35641 gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg
    35701 agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg
    35761 gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca
    35821 tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat
    35881 tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca
    35941 tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca
    36001 gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc
    36061 tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct
    36121 ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga
    36181 aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa
    36241 aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg
    36301 ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac
    36361 accttataca cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt
    36421 gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt
    36481 gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg
    36541 gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact
    36601 agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc
    36661 tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc
    36721 gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag
    36781 gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc
    36841 acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa
    36901 agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta
    36961 cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct
    37021 ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca
    37081 tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt
    37141 attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta
    37201 aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga
    37261 aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg
    37321 acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg
    37381 atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga
    37441 cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt
    37501 ttcttttagg aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc
    37561 ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc
    37621 tgctgtttct gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca
    37681 tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag
    37741 gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat
    37801 tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag
    37861 ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc
    37921 aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg
    37981 cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac
    38041 gttctatgca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc
    38101 tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa
    38161 atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc
    38221 ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc
    38281 tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga
    38341 atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc
    38401 atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac
    38461 tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat
    38521 gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc
    38581 ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa
    38641 gcaaatggag cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag
    38701 acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga
    38761 aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta
    38821 tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa
    38881 aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca
    38941 ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc
    39001 gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatcgaat cgctgtgatc
    39061 caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc
    39121 aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta
    39181 ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag
    39241 gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt
    39301 cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat
    39361 gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac
    39421 gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa
    39481 gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata
    39541 cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt
    39601 cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt
    39661 acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga
    39721 gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat
    39781 ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga
    39841 ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac
    39901 aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc
    39961 ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa
    40021 cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc
    40081 tgcattagtc tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc
    40141 aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa
    40201 actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga
    40261 tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg
    40321 attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca
    40381 aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta acactactga
    40441 aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa
    40501 gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat
    40561 agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct
    40621 cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat
    40681 ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca
    40741 aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag
    40801 atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga
    40861 taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa
    40921 cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt
    40981 tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg
    41041 tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat
    41101 tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc
    41161 cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga
    41221 ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt
    41281 tttgtatatt ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct
    41341 gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc
    41401 ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca
    41461 gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata
    41521 gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct
    41581 ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg
    41641 aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa
    41701 ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc
    41761 aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct
    41821 tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca
    41881 agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata
    41941 gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt
    42001 aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca
    42061 ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc
    42121 ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca
    42181 attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa
    42241 acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca
    42301 gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa
    42361 acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa
    42421 gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc
    42481 ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa
    42541 attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca
    42601 aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg
    42661 attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag
    42721 attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg
    42781 ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag
    42841 tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa
    42901 gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg
    42961 tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg
    43021 ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt
    43081 aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact
    43141 ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg
    43201 acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa
    43261 tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc
    43321 ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt
    43381 aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg
    43441 aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa
    43501 ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag caatgttcag
    43561 cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc
    43621 atgtcctttt tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc
    43681 tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc
    43741 aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg
    43801 tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg
    43861 tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc
    43921 gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg
    43981 ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag
    44041 caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc
    44101 aactagcttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat
    44161 atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt
    44221 ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg
    44281 aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt
    44341 ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg
    44401 catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg
    44461 aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc
    44521 atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt
    44581 catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta
    44641 ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt
    44701 tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca
    44761 agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg
    44821 aatgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca
    44881 agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa
    44941 gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga
    45001 agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt
    45061 ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac
    45121 aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa
    45181 atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg
    45241 gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt
    45301 gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat
    45361 aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag
    45421 caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc
    45481 cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc
    45541 gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa
    45601 gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat
    45661 gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca
    45721 aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga
    45781 tgacacagaa gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac
    45841 tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg
    45901 gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga
    45961 agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt
    46021 attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac
    46081 aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga
    46141 acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt
    46201 gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca
    46261 agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga
    46321 tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca
    46381 gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt
    46441 taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga
    46501 atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc
    46561 ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc
    46621 tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta
    46681 cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag
    46741 aaagtcctga ttggataagt tggattacca gatgtcattc agataattct gttccaaagg
    46801 acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg
    46861 tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca
    46921 gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa
    46981 tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc
    47041 gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg
    47101 ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt
    47161 gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat
    47221 ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg
    47281 gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt
    47341 ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga
    47401 aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca
    47461 gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag
    47521 caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg ttcttcaaaa
    47581 aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct
    47641 tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta
    47701 ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat
    47761 cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat
    47821 tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa
    47881 aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca
    47941 aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta
    48001 tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac
    48061 agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg
    48121 caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga
    48181 cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt
    48241 tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg
    48301 aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt
    48361 ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga
    48421 cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc
    48481 tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg
    48541 tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc
    48601 tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag
    48661 atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata
    48721 tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc
    48781 caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat
    48841 aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct
    48901 gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc
    48961 ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt
    49021 gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc
    49081 tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat
    49141 ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa
    49201 gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac
    49261 tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata
    49321 aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga
    49381 tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga
    49441 cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag
    49501 gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat
    49561 tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa
    49621 aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact
    49681 tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt
    49741 ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa
    49801 gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta
    49861 gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata
    49921 gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt
    49981 ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa
    50041 aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt
    50101 caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca
    50161 agctcttttt tattttagga attagagagg gatttgtcct tctatgattt tttgctataa
    50221 tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg
    50281 atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc
    50341 tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca
    50401 ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt
    50461 ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt
    50521 cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca
    50581 ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat
    50641 gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca
    50701 atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc
    50761 gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact
    50821 gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta
    50881 gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat
    50941 tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt
    51001 cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac
    51061 caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg
    51121 gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc
    51181 atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat
    51241 cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt
    51301 ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat
    51361 gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta
    51421 ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta
    51481 actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc
    51541 ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat
    51601 cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta
    51661 aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg
    51721 aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga
    51781 agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg
    51841 aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc
    51901 acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt
    51961 ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag
    52021 gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt
    52081 atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt
    52141 tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa
    52201 gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg
    52261 aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt
    52321 gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac
    52381 tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat
    52441 gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga taatacctac
    52501 gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt
    52561 gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa
    52621 attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt
    52681 attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat
    52741 aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa
    52801 atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc
    52861 attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tattccaaca
    52921 tcatcttgga ctttctacat gttactaatg agcgtgtaaa agaacgagat ggtgttttta
    52981 tttctatcaa tgaagctctt tttctttgca atcctcactt caatcctcta taatgtcgca
    53041 tatagtttag taaatgagta aactactaat ttacttattt actaaaaata aaaggatgag
    53101 gagttaaaat aatggaaata ccaacaaatt taaaacataa accagtaatc gttgtagaag
    53161 actatgataa agttgatgga agaaatgcat taaatacgga tgcaaaaggg ctttcactgg
    53221 gccttgctca gtggaatgat cgtggaaaag tcgatatctc aggcaaggta tggcgacata
    53281 ctggagaaaa atggtcacga cagtcggaag aattaccaat cacacgtatt ttagatttag
    53341 cgatcttagc tgcacaagga agtttatact tccaagatgc ttatcgttat gaaaaatttt
    53401 atgatccaga aaatccagtc gtagacatca ttggattaca agggaaccga atgacggtag
    53461 aagtagatac caaaaatcca aagatcgatg aagacatcat tttgtattac gatacactac
    53521 aaaaagacgg agaactgatt ggggaacgat tccgtatttt aaaacgtctt ttagatgagc
    53581 taggatatta atagacagag atgatcaaaa ggggaagaga agcatggaga atttctcact
    53641 aacagaaaac cgaacctttt cacggctaga aaagcaaatg atctggaaga aaccgaaaag
    53701 ccatcggaca agtgcagaag aatggcggat cgcctccgaa atcaaaggga attggcaaga
    53761 tcctgaaatg aaaatcagta acgtactttt ggaaggagat gccgggtcgg ggaagacaca
    53821 attagccaaa gcactatctg ctgatctgca acttccctat acaaaagtga cctgttttgc
    53881 agatatggac aaatcagatg ttttcggagc cttattacct gtagtagatt ctaataagga
    53941 acaagaccaa gaattactag aagctattta ccaaacagat acacttgaag ctgtactgac
    54001 actgatccag aaccattacg gcattgatcc tttttctgct aaagaaaaat tagcagagct
    54061 agttcaaagg atcgaagctg attatccaga tgcaatccat tacagctatt acccttctga
    54121 aatcgttcga gcgattgaaa aaggatattt attagagatt caagagccga ctgtcatccg
    54181 agatgcctct gttcttgtgg cacttaactc agcgctcgaa ccaaatggga tgttgaattt
    54241 accgacagga atcattcgtc gccatccaga ttgcgtcatt gtgatcacta caaatcgaaa
    54301 ttaccaagga aatcgcccat taaatgaatc tttaagagat cggatgcaac atgcggaaaa
    54361 aatggatctt cctgccttag aagtcatggc agaacgtgcg atggcgaaaa ctatgatcca
    54421 aaaacaagaa cttctcttaa agatggcaga gattatccgt ttgttggatg aaactgcaaa
    54481 agctaacgcc atcaaaggtg tagcaggaat gcgttcttat ttttattggg caaataccta
    54541 taagcaagga caagatttac tccagtcgat ttatccaaaa gttttgtata aattatccac
    54601 tgattcagat gaattggcaa ttcttgaaca agctttgtct gacagtggat tgctactgga
    54661 acttcaggac atattgagaa aagaaaaatg gggtcatcct caatctgagc atacaaaagg
    54721 aagaaccatt agcgccgaag aagcaaatga acgaaacatc actgaaaatg ctgaagaaat
    54781 tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg gagaagagaa
    54841 acaagaagaa acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag aacgcagtag
    54901 cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga gatgattctc aagaaatgtc
    54961 tgcttctgac atggaagcac atgataagga aatcaagaag caactgaata aagaagcgcg
    55021 acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc gaccagcttt
    55081 ccaaggaaaa acgccggaag cacgtgagct acacatgcaa gtaatgccaa tagtagaatc
    55141 acttagccgg caaattttag agttactgga taatgaacaa acagagacgt accaaaaagg
    55201 aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca tatggtgatt tgagaaattt
    55261 tgacaaaaag aacccgccac atgagcaacc ttctttagcg gtagctgtac ggatcgatga
    55321 atctgggtca atgatacggg atgatcggat cgaagcggca aaaaaagcag cgattgccat
    55381 tgccgaattt tctaagaaag tcgatatccc attgttgatt tacggagata cagcagaccg
    55441 ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa tttgaagacg gatttcaatg
    55501 gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg gtgctgcact
    55561 tcgtttagca gcagaaaaat taaacagaca atccgcaaca accaaactgt tgcttaatat
    55621 tagtgacggt cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa aagaagatat
    55681 ccagtctgtt ttgaaagaat atgaacgtca aggaatccta tttgtctccg cagctattgg
    55741 ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt cgttttgtag atattactga
    55801 cttgaatgaa tttcccaagc aaatgattca actgattgcc cgctatctat gataaaataa
    55861 agctcaacga acgcacttag cagattcgtt gagcttttcg tttctagtta ccggtaacaa
    55921 ggatacttct aaaatagaat cggttataat caagagggac cgttaaagga gtgaaacgat
    55981 atgatacaag aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta tcctgctagt
    56041 ttcaaagata gcaatggcga tggtcaagga gacatcaatg ggatccgtga aaaacttaat
    56101 tatttgaaag atcttggtat cggctttatc tggatcaatc cgatctatca atctccattt
    56161 gtcgataatg gttatgacgt aagtgactat caagatatag aagagagatt cggaacgatg
    56221 gaagattttg atttattgct aaaagaagca catgaattag ggatcaaagt gattatggat
    56281 cttgtcatca accatagttc ggatcagcat caatggtttg aagaatcgaa aaaatctaaa
    56341 gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg tagatggcaa agaaccgaat
    56401 aattggacat cgattttcgg cggttctgct tgggagtatt ctcatgaaac tggacagtat
    56461 tatctgcatg tttttgcaaa agaacaacca gacttaaatt gggaaagcga gaaattgaaa
    56521 gaagaactat tcaatatgat ccgatggtgg ttggataaag ggatagatgg atttcgttta
    56581 gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga gaaccctttc
    56641 tcaccctttc aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa acatgttttt
    56701 gaagagtacg atatcatgac tgtcggagaa gcaagtggtg tcactgcaga agaaggacca
    56761 caatgggtag ggaaagacgg ttatttcgat atgatattcg aatttgatca tatccatatc
    56821 tggcagcaag aaaaagaagg gcaattagat gtccttaaat tgaaacttgc attaagcgct
    56881 tggcaaactt cattggatgg tatcggatgg aacgctttat atatggaaaa ccatgatgtt
    56941 cctcgtgccg tttcagtgtt tggagatact cgtcccgatt tttgggctat gtctgcaaaa
    57001 gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat ttatttatca aggacaggaa
    57061 atcggaatga cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt agattcgaaa
    57121 agactttata aaaggctgct tgcggaaggt aagacaagag aagaagcatt ggatatcatt
    57181 cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat ggacttcgga acaatatgct
    57241 ggtttttcta cacatgaacc ttggctgatc actaatccaa ataccaaaac gatcaatgta
    57301 gaacaacaag aatatgaacc agagtcggtc ctgcaattct ataagaacat gatccgcatt
    57361 agacaaacca ataaaggtct gatatacggt tcttataaag aatatcttca tgaacatcca
    57421 caactttatg tctatgaaag atatttagaa gatgaacaat atcttattat ggtaaactta
    57481 acagaatcac ttgctgacta tgaacttcca aaagaagcag atcagtcctg gacattactc
    57541 ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca tactagcacc ttatgaagct
    57601 cggttatata aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt ggactttgcg
    57661 aggaagtttt tcagattgct ctgtcaatta ctcatttcaa taattcgctc taggaccacc
    57721 gatatattta ggacgagagc gaagggctgt cacgtgagct tgtccaaggg ttttcaaacg
    57781 aggtctgaca ggtacttgga catgtttgat atgcataccg attgatgtgt caccgatatc
    57841 gataccggct tgtccaacta cgtgttccac ttcgacagga tcattgaact ggtcaaaagc
    57901 agcaacagaa caagcacctc cagcatgtag tgcaggaaca acagagacaa tttcgaaatt
    57961 ttttgcttct gccactgttc gttctactac taatgctcgg ttcaaatgtt cacaaccttg
    58021 gacagctagc gcaatttctt taggatccaa caactctttt aacgtacgga tgatccattg
    58081 accaacttcc gcactggaat tttttccaat atgtcctcca acgacttcgc ttgtgctgca
    58141 tcctaaaaca aaaagatcac cttttttcaa gtgtgcctca gccaatatgt cattaactat
    58201 ttcagtcaat tggtctttta attctttttc tgacaacttc attttctaaa accttctttt
    58261 tcaattaata ttgcttccgt gcttgaatac gttgaagact tgcagttata ggaaaggcaa
    58321 gtcccattcc aatcagattt tggaagatgt ttcctggtat agaagccact ccagccggcc
    58381 aatcatataa aatccaaccg gcgataccat agccgatgat cataactaag cttcctaata
    58441 aaagccctaa tccccacata cgagtagaag atttgctgaa agaaccagca acatatcctt
    58501 gtaacccgtg gatcactaat gaaaaaactg cccactgcgg atatcctgaa atcaaatcga
    58561 tcaatagacc actggaagca ccaacaacta atccacccgt tggaccataa agaaaagcag
    58621 cggtatagat cccggcctcg cataatgtca caaatccatt cgtagccggt acagggatca
    58681 aaattgttaa tgaaagggca actgtcaacg caatcagcat agcatagatc gttaattgct
    58741 tagttttcat ttctcattcc tcctttttga ataccgccag accagacact tccggtatga
    58801 tcgttcatca gcacgccatt agaaatacaa tgggcaacaa attttttact ttctgctact
    58861 gctttggtaa aatctgcccc taacacaaga ttcgctgtta ttgcagaaga aaaggcacaa
    58921 ccggccccgt ttactgttgt agtgtcgatt tttttatgag aaaatacctc ataatgatca
    58981 attgaataga gaagatccac agcttcatct ccagccatcc ttgtgccgcc tttgatcaca
    59041 atgttttttg ttcctgaagc agtgagttct ttagcgagat ggatcatttc tgttaacgaa
    59101 gagggaagag cttgaccaga aagtaaagca gcttctgcca aattaggcgt aatcatcgta
    59161 acaagtggaa aaagttcttc gatgagctgc gaaatataca attgattgat cacagcttca
    59221 gtttctttga atgccaaaac agggtctaaa atgattggga tattttgttt tgtttttatg
    59281 aagtctcgaa caaccatgat tgcttcactt gaattcaata acccaatttt gataccatct
    59341 aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa agatatagat
    59401 tccagcataa atcgctcatt aatatctagt gcccctatag aagtcaaggc gcttagtccg
    59461 aatagaccaa aattctcaaa agtttttaaa tctgtttgga ttcctccacc agcaaatgga
    59521 tcagaaccgc caattgtcaa gattcttttc ataacatttt cctccatgaa taagagtata
    59581 acgaaatttt caaaaaagaa aaacagccaa ttggcatatt tttatctcat ccaatttaaa
    59641 aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt ccttaaatag ttaggacttt
    59701 tgtgatttta attctttctc gatttgaagc cgttgcactt tcattgtagc tgttcgcggg
    59761 attgcatcaa aatcacggat gatagggacg ttcaagcgag gaagttcatg gacttgctcc
    59821 caccatcgat tccaatccat ttctttatca ggagctaaag caataatcgg ttgtggagaa
    59881 tgatttttat cccttacaat cactacttct gccaaaaaat ctaacgaatc taataaaaag
    59941 tcttcaatag ctaatgtact attgatcgtt tcgatcaaat ccacttgccg atctttcaaa
    60001 aataggtggc catgcttatc cataaaaccg taatctccgc tatcccacca atcgccataa
    60061 acattttcct ggaaccgtgc atcttccttg taataagtca aagcccgtcc tttagacagt
    60121 aattgtatat gaccatctgt gttttcaggt aggacattcc caactgaatc tgtaatccga
    60181 gcgcttgtca tgtcttccag tccaacgccc atatctcttg catcacttgt tttcaaactc
    60241 tctagtgtat gagccttaag aatcattggt ccgcattcac tttggccata tacttgaaga
    60301 aataccgcat cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg gttgtttata
    60361 gcatcaaaag tggagtgata gtatcggata cccgaaaaag cttctggatg ctccttagca
    60421 gtaaaacgcc actgaacaaa gttattcgga tgggtctcta gtgcaattgg ccggtgagct
    60481 tccaacatat gaacaacttt tgaactttgt gcgtttgcta aaggcatcat aggaaatccc
    60541 atggacatca aagaagagat accaatattg aatctggaat gcactggaga gatgtgaaaa
    60601 gcgattaatt tcttttccga aatttttgtg aaaactgttt tttgccattt cgtccgccat
    60661 cccatagaat gagcagaatg acaaataagt ttagggatac cagttgtacc agaagtatgg
    60721 gtcatataag aaatttcatt tttgtctaaa aatgattgag aaacagacaa agcaggttgt
    60781 tccattaaat aaagaatagg aa
//
LOCUS       DAHHPA010000005        59739 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.005, whole genome shotgun sequence.
ACCESSION   DAHHPA010000005 DAHHPA010000000
VERSION     DAHHPA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.005"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="NBH55_000276"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="NBH55_000276"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCD1508292.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="NBH55_000277"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="NBH55_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCD1508293.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="NBH55_000278"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="NBH55_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCD1508294.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="NBH55_000279"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="NBH55_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCD1508295.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="NBH55_000280"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="NBH55_000280"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCD1508296.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="NBH55_000281"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="NBH55_000281"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCD1508297.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="NBH55_000282"
     CDS             complement(8054..8524)
                     /locus_tag="NBH55_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCD1508298.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="NBH55_000283"
     CDS             complement(8570..8995)
                     /locus_tag="NBH55_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCD1508299.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="NBH55_000284"
     CDS             complement(9062..9331)
                     /locus_tag="NBH55_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCD1508300.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="NBH55_000285"
     CDS             complement(9342..10937)
                     /locus_tag="NBH55_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD1508301.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="NBH55_000286"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="NBH55_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCD1508302.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="NBH55_000287"
     CDS             complement(14490..15050)
                     /locus_tag="NBH55_000287"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCD1508303.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="NBH55_000288"
     CDS             15403..16377
                     /locus_tag="NBH55_000288"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD1508304.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="NBH55_000289"
     CDS             16547..17947
                     /locus_tag="NBH55_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCD1508305.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVNLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="NBH55_000290"
     CDS             complement(18025..18747)
                     /locus_tag="NBH55_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1508306.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="NBH55_000291"
     CDS             complement(18826..19332)
                     /locus_tag="NBH55_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCD1508307.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="NBH55_000292"
     CDS             19455..21254
                     /locus_tag="NBH55_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCD1508308.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="NBH55_000293"
     CDS             complement(21322..22896)
                     /locus_tag="NBH55_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCD1508309.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="NBH55_000294"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="NBH55_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCD1508310.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="NBH55_000295"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="NBH55_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCD1508311.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="NBH55_000296"
     CDS             complement(24673..25311)
                     /locus_tag="NBH55_000296"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCD1508312.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="NBH55_000297"
     CDS             complement(25312..25962)
                     /locus_tag="NBH55_000297"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCD1508313.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="NBH55_000298"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="NBH55_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCD1508314.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="NBH55_000299"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="NBH55_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCD1508315.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="NBH55_000300"
     CDS             complement(29105..29845)
                     /locus_tag="NBH55_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCD1508316.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="NBH55_000301"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="NBH55_000301"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCD1508317.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRKGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="NBH55_000302"
     CDS             complement(31216..32154)
                     /locus_tag="NBH55_000302"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCD1508318.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="NBH55_000303"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="NBH55_000303"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD1508319.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="NBH55_000304"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="NBH55_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCD1508320.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="NBH55_000305"
     CDS             complement(35314..35619)
                     /locus_tag="NBH55_000305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCD1508321.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="NBH55_000306"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="NBH55_000306"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCD1508322.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36725..37009)
                     /locus_tag="NBH55_000307"
     CDS             complement(36725..37009)
                     /locus_tag="NBH55_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508323.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37027..37917)
                     /locus_tag="NBH55_000308"
     CDS             complement(37027..37917)
                     /locus_tag="NBH55_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCD1508324.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37937..38155)
                     /locus_tag="NBH55_000309"
     CDS             complement(37937..38155)
                     /locus_tag="NBH55_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCD1508325.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38125..39825)
                     /locus_tag="NBH55_000310"
     CDS             complement(38125..39825)
                     /locus_tag="NBH55_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCD1508326.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40092..40865
                     /locus_tag="NBH55_000311"
     CDS             40092..40865
                     /locus_tag="NBH55_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508327.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40946..41515)
                     /gene="thiT"
                     /locus_tag="NBH55_000312"
     CDS             complement(40946..41515)
                     /gene="thiT"
                     /locus_tag="NBH55_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCD1508328.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41581..41683)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41689..42660)
                     /locus_tag="NBH55_000313"
     CDS             complement(41689..42660)
                     /locus_tag="NBH55_000313"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCD1508329.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42754..44127)
                     /gene="glmU"
                     /locus_tag="NBH55_000314"
     CDS             complement(42754..44127)
                     /gene="glmU"
                     /locus_tag="NBH55_000314"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCD1508330.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44630..45448)
                     /gene="purR"
                     /locus_tag="NBH55_000315"
     CDS             complement(44630..45448)
                     /gene="purR"
                     /locus_tag="NBH55_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCD1508331.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45615..46439)
                     /locus_tag="NBH55_000316"
     CDS             complement(45615..46439)
                     /locus_tag="NBH55_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD1508332.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46412..47092)
                     /locus_tag="NBH55_000317"
     CDS             complement(46412..47092)
                     /locus_tag="NBH55_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD1508333.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47115..48053)
                     /locus_tag="NBH55_000318"
     CDS             complement(47115..48053)
                     /locus_tag="NBH55_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCD1508334.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48298..49161)
                     /locus_tag="NBH55_000319"
     CDS             complement(48298..49161)
                     /locus_tag="NBH55_000319"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCD1508335.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49303..51876)
                     /gene="mprF"
                     /locus_tag="NBH55_000320"
     CDS             complement(49303..51876)
                     /gene="mprF"
                     /locus_tag="NBH55_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCD1508336.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52017..52823
                     /gene="proC"
                     /locus_tag="NBH55_000321"
     CDS             52017..52823
                     /gene="proC"
                     /locus_tag="NBH55_000321"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCD1508337.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52902..54458)
                     /gene="rny"
                     /locus_tag="NBH55_000322"
     CDS             complement(52902..54458)
                     /gene="rny"
                     /locus_tag="NBH55_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCD1508338.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54869..55918)
                     /gene="recA"
                     /locus_tag="NBH55_000323"
     CDS             complement(54869..55918)
                     /gene="recA"
                     /locus_tag="NBH55_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCD1508339.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56027..57268)
                     /locus_tag="NBH55_000324"
     CDS             complement(56027..57268)
                     /locus_tag="NBH55_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCD1508340.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57508..58086)
                     /gene="pgsA"
                     /locus_tag="NBH55_000325"
     CDS             complement(57508..58086)
                     /gene="pgsA"
                     /locus_tag="NBH55_000325"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCD1508341.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58295..59218)
                     /locus_tag="NBH55_000326"
     CDS             complement(58295..59218)
                     /locus_tag="NBH55_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508342.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT        16482 a        13030 c        10714 g        19513 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaata aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc aatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc acccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatc ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggtaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cctagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc ctttacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcatcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg ctttttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ccaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgtttttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgacatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac cgagaaaatg gtttttgaac atcacttatt
    36601 caaaataaaa aaagattgtg acacgaattt tgtcacaacc tttaaatgta ttcctagagc
    36661 aaagaagcta agcgaagaaa tccaattctg tttatttatt gtatttcttt gttcagcatc
    36721 cttgctaaaa gcccatattg gacaaagtat tcactacacc catgatctca tcttttcgag
    36781 aacgtgcttt ttctgtcgga tcttctacct cttctgtgtc aattcgaaca ataaatttcc
    36841 gattcatcgt cacgtaagca ccgtcgttga tcaaaataaa cagttcgttc tcattatagt
    36901 ctgcacaggc atctttccat acgtcaaagc cgtttttttc agaagggata tcccgtttaa
    36961 ccacagtttt tccacttaat tcgtggaacg ttacgtcata atatttcatt atctctacct
    37021 ctttcgtcat tcaatatttt gtacttgttc tctgattttt tctaagatcg tcttagactg
    37081 gacgacttga tttttaattt cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg
    37141 attcatttct tggatcagga aatctaattc ccgacctaca ggacattgtg cttcgataag
    37201 ttcatctagt ttttgtacat ggatcgccag acgatccagt tcctcgtgaa tatctccgcg
    37261 ttccaacaag atggctagct ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa
    37321 ttcctccagc tttttttgat accttatttg aaattccttt tcatagattt ctacaaaact
    37381 atcgagtttt tttagtactt ctttgaattc ttcagtattc tttttcaaca ctaaagctaa
    37441 agcttgacct tctttttctc gactcgcctc aatagaatca agtgcgcttt tcaatgtatc
    37501 tagagctaac tgtgccaatt cttccatgct ctcttccggt ttttcttcta ccgttacgaa
    37561 gttttcgtcc agcacgaagc gttccgccat ccgactaatt gacacttgat tttgactacc
    37621 gtatcgttgt tgaattccgt ctgtcaattg agtgataagt gaatcaatca attcccagtt
    37681 gatgacgatc tgtttttcgt ttttgccaat ataagctaga ttcacaaata cttcgattcg
    37741 accgcggctt aactgctgtt taataagctg acgaaaatcg ttttcaacaa agttcaggat
    37801 tttaggactt cgtatttgaa catctagaaa acgctggttg acacttttta tttcaatatc
    37861 taactgatac gcttttgttt cgcggatggc tttgccaaaa ccagtcatac ttttcattcg
    37921 acagggtcct tcctcattac atattttcta ttgctttttg cagttgttca aactcttcgt
    37981 tcgtcaatgt caggcctttc cccatttttt catggtctgg cgcccaatct cgaagatcga
    38041 attttggcgg gcgaccattc cagctgacta ggttcagttc tttacgccac cccttgttgt
    38101 tttctgacaa tatagcgatt tcttctacga tttcatatga aaattcttgt gccatggttg
    38161 ttcctccttg agataatata aatataggat cagtaatgtg tcaactggtt gcttaagcaa
    38221 ttcatgctgc aaacgatcct tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt
    38281 cgtgcgctca ttttgtattt tttgctggta ttttcgatac cttcgccatt cttcagtact
    38341 taattgacca atttgtgcca ataaatcatc cggcatactt tttatatctt gttggcagat
    38401 catttgaatc atcatgtcta cttgatagat ttcattgggc agtaaaaaat gtttttttgc
    38461 taaaagttct agcatcgcca atccgcatgt ctctggtacg accctgttct tatataaatt
    38521 acgcagtttt tgccaaaatt ttatgctttt gaaaaaagat aattctaact gaatttcttt
    38581 tgttaagtaa gacaaaaatc ctcgccagta tgttcttgca ggtttaacag aatgaaatgt
    38641 ataccagttc ccatagtaat agcctaatcg ttttttcatt tccagatagc ctaattgtaa
    38701 atttaattgg ttcctttggc tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg
    38761 acatttccaa tggataaaaa tgtctggcat acgaattttt ttcgctgggc ctggcccttg
    38821 cacatctacg acaaatgcct ctgttgctcc tttgttaatc gcaatatcaa taggtatttt
    38881 gttgcgatac ccaccgtctg catatttatt ttttccgatc tttcgataag ccatagcggg
    38941 ataaaatgaa gcagaggcga gtatccaatc tggtatttct tctgtttttg tctgttggat
    39001 atcagtcact acttccgcta gttctggcag tttagtagac accgtatata aacgtatttt
    39061 gggattatgt ttgagtaagg acaaatccaa attgtctttg accatctttt ccaatggagc
    39121 agaagaaacc ccgccttcaa ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa
    39181 gcgttcacga aggtcttcaa ctgcagccat ttctggtaac gcaagcactt gtcttgtcga
    39241 cagctttttc cataaattca cagctttttt catatctcct tgtaaaatca atgcgccgtt
    39301 caacgcacct accgaggtac ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc
    39361 ctgccacaca ccaatctgat aagcaccgtg cgctcctcct ccacctaaaa caagtgctgt
    39421 atgatagacc agctttttat ttaattgtgt ggttccttga tagccgtttt tatgaaaaat
    39481 acgcaaccat tcttctgata gaggagattg aaataaaaaa caacagtttt ctttaataaa
    39541 aaaacgagcg caagcttcca tataccgaaa aaattttttc cattcaaaag tttcaccttc
    39601 aatcaacaga ttattaaaaa taacatctcc tctactttta gcaatagtaa aatagacggt
    39661 atattttttc gatactgctt taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc
    39721 tctactttct tgatgacttt gaaaaaatgg aaggggttgt tttgcttgct ggataggcga
    39781 atacagcgac attttctgtg aaaagtaaac tttttttatc tccatcgcgc tctccttttt
    39841 cttcaagtat accagtacgt cataaaaagg tcatgagtct cgtattcgac tttgtagatt
    39901 ccataagaaa acaattcata aaacctcctt taaataaaaa aatacaaaaa tcacaaaaat
    39961 taattgaaaa tgagagtgtt ttcaatgaaa acgtgatttt gtaatggaat tttaaaataa
    40021 aagaaacaag cttttttaat gcaaatgttg taaaataaaa gagattaaga ggaaataaga
    40081 ggaggaacat gatgactgag tcagtttatg tacatattga taccacaagt aatgctgttt
    40141 taacaaaagg gctgactgct actgactttg caaacagtat cgtgcatttt cctcaaaact
    40201 tgctcttgct tgatccatct gcttctgctg gagaatatga atcccataca gggctgaaag
    40261 taatccgtgg tacagaaaat attcagcgtt tcttttcctc ttctcgtaac aggcgtggat
    40321 tcgatgaatt gaagtggatc gactttactg atttaacgat gctgaaagaa ttgacacctt
    40381 tagaaatttc agaattatta tattttggtc atatgaaaac acatcttcat tcgccgtttt
    40441 tttataaact acagaataat tttgtatact tcgatcttaa tgatcagctg aatcggattt
    40501 attatcgcta tttagatgaa ttttatagga tttttgctag taaattaagt tcgatcttgt
    40561 cagataagat caatgtaaaa aaatcttttt tcaaaaaaga aacacctgtt gaaaaaatca
    40621 gtgtggaatt attgaaagaa atgcgtaatt tgatgcagga agggatcatt ttttcttttg
    40681 atcaagttgg tttgatcgat ggtaaatatc gtatcccaat ctatgtgatt gaagattata
    40741 tttggaaatt aaaaggcacc cggtatcgag aagaggaaat catcgctact ttaatctact
    40801 ctgtcaccga tcgacactgg caattggtag aagatgccga tctttcttat ctgccattga
    40861 agtagatcta tttcttatca aaaaataaaa aggaaacaag aaactaggac atgattctgt
    40921 ctcaagtttc ttgcttcttt tttatttaca gctcctgtcg tttatctggc acaaaaatcg
    40981 aaggagtcgt ttgttggatc cctaataaaa cgatgatcgt tacaatagct gtactcaacc
    41041 cgctggcacc attcattacg aaagaaaaga gccaaggatt catcccccat agtgcatacg
    41101 ctccccagaa aaccactcct gcaataaaat gccaaaaata tctggaaagt gcgccaatca
    41161 atgcaccaaa cacgatgctt tttctaattt gtttcgtatt atcgtctaca tagccttttt
    41221 ggacatttga agccatgagc cctgctgcgc ctgcaaaagt ataggcaatc gggtactcaa
    41281 tcaacacttg aacgacactt aaataataaa cttgtcccaa cggaaaatgt aggatgcccc
    41341 aaatgaaacc tgccagcatc cctgcctttg tccctcttct taacgcaaaa agagtcaatg
    41401 gaatctgccc taatgaaata gaaaaactgc tgccgatctg gatcggaatc aacgacaaca
    41461 ccatcgctaa cgctgcaaca atcgttcctt ctacccaaac tctatttttt cccataaaaa
    41521 aacttcctcc tttgaataaa aaagtcacaa aggaggaagc agctttcgaa aatagtcaaa
    41581 cagctgttta ttcacaattc ctacgctcgc ataaccgata caggtacgaa gggtttagaa
    41641 atagattcaa tctcagccca attggactcc cctttgtgag tttatttatt atttattttt
    41701 tgtttcaaac aatggactaa ctggtttgtt ttcatggata cgtttgattg catctcccat
    41761 tagtccgcca acacttactt catcgatttt gtcgatttta cgatcttctg gaagatagat
    41821 cgagtctgta acaactagac gctcgatagc tgaatcttct attctttgta aagcagggcc
    41881 ggataatact ggatgtgtac atgaagcata cacactagtt gcaccagctt ctttcaaagc
    41941 attggcagcc aatgtgatcg tacctgctgt atcgatcata tcatcgatca gtacacaagt
    42001 tttgcctttg acttgtccaa tgatattcat tacttctgca acatttgctt ttggacggcg
    42061 tttgtctatg atcgcaatcg gagatttcaa aaattccgct aatttacgtg cccgtgtgac
    42121 accaccatgg tctggtgaaa caacgacaac atcatcgcct ttgattcctt tttctaagaa
    42181 ataattggca ataagtggcg cacccatcaa atgatcgact ggaatatcaa agaaaccttg
    42241 gatttggact gcatgaaggt ccaaagtcag cattcttgtt gcaccagctt tttgaatcat
    42301 gtttgctact aatttcgctg tgattggttc ccgtgcacgt gctttacggt cttgacgcgc
    42361 atagccataa taaggcatga ctacgttgat tgtttttgca cttgctcgtt tcagtgcgtc
    42421 aatcatgatc agcaactcca tcaagttatc attgactggg ctgctcgttg attgaataac
    42481 atagacatgc gatccacgaa tactttcttc aatgtttact tgaatttctc catcactaaa
    42541 ttgagtaaca gatgattttc ctaactcgac gccgactgct gctgcaattt tctctgctaa
    42601 tgggcgattg gaatttaacg caaaaatttt taatcttggg tcaaagtaat gttttgacat
    42661 ggggacctcc actttctttt tcacaactct agaattctac ctaataaata gtagtcatat
    42721 tttttaaata aatcaagtga ttttgcaaaa gtcttagttt agataaggga gtttttttgc
    42781 atatccttct ttattgactt gtcttgctct cgcaattgcc agcgcatatt ctggaatatt
    42841 gtctgtaatt gttgacccgg cagcaatcga actgttttta gctacctcga caggtccgat
    42901 gatattcgtg ctagaaccaa taaagctgtg gtcgccgaca gtcgtatgat gtttgttctt
    42961 tccatcatag ttgacgaaga caacaccaca tccgacattg atttcttctc ccagcgtagc
    43021 atcaccaaca taagtcaagt gaccgacttt agttcctttg ccgatttgag catttttcac
    43081 ttccacaaag tttccgatat ggacgttttc gccaatttca gcttttggtc gtaaatgtgc
    43141 ataaggtccc acatctgcat gtttcttgac atggctgctt tcaatgactg agttttcaat
    43201 gacgacgtga tcctcaatcc tactgtctac gatttttgaa tgtgacccga tgacacaatc
    43261 agaaccaatg actgtatttc cttggatttg tacacctgct tcgatcaatg tgtctggacc
    43321 gatttctacg cctgcatcga tgtatgtcgt tgctgaatcg acaaagctta cgccattgac
    43381 catatgcatc tggttaattc gctttctcat gatctcattt gctttagcta acgcgatccg
    43441 atcattgact cccattgatt cttcaaaatc atccgtttga taagcagcaa ctgtatggcc
    43501 ttcttctttt aagatttcga tgatatctgt tagatagtat tcaccttgag cattgtttgt
    43561 tccaactttg cttaacgcat cgaacaaagc ttcgttgtca aaacagtaag ttcctgtatt
    43621 gatttcttgt acaagagctt cttctggtgt tgcatctttt tgttccacga ttttttctac
    43681 gatcccaata tggtcacgga tgattcgtcc atatcctgtt gggttttctg cttgtgccgt
    43741 caagatcgtt gcacttgcat tttttccttg gtgatattca aaaagattgt tcagcgtttc
    43801 agtagtcaat aatggtgtat cgccactgat aactaacgtt gttccttttt ttccttccaa
    43861 gaaagaagca gcttgcaaaa cagcatgtcc tgttcccaat tgttctgctt gtaacgcata
    43921 tttactgcgt tctcctagtt gtgctttaac ttgttctgct ccgtgtccaa cgatcgtaat
    43981 cacttcatct ggtttcgttt cactgacccg attaatgata tgttctacca taggtttacc
    44041 agaaacggga tgtaaaactt tatataattt tgacttcatg cgggtacctt tccctgccgc
    44101 caaaataatt gcataacgtg cgtccaactg cgtcactcct aaatatagtt tgctgatttc
    44161 agtttatccc agtataccca ttttttcaac aaaacaagtg attttctttt atcccagcgt
    44221 tttctggata aaaaaataga tagaccataa cgggcctatc tatttggtaa ccggtgctct
    44281 cctgccaact cctcggtcac aagtcacact actcataaaa catgaaaagc tgttttttga
    44341 aatcgtttct tgtgacttag agttaaacgg taggcttaca acctcttcat taaacgtttt
    44401 tcaatcagct gaccttcggt attagctcaa taaacggcaa aatatgagga gcaatttttc
    44461 cgtttattga gctaatactc aggacaggac gctgatttca caacctctag ataaacggtg
    44521 ttcaaaagcc agtttctcgg tattaagcca aatttatgaa aaaatgtgga aagccatttt
    44581 cacaaaattt ttcttactac tcgaaactaa gcggcttttt cacaacctct tattctgaaa
    44641 aataatttcc tggcacaacc gtaatatttt tcgtttgagt atctacatct ttgacataaa
    44701 gcagtgacgt ataatcatcg atggcgcgat gaccgttgaa ttttgattcg gcaaatacag
    44761 tgatgccaac aagttctgat tcgaattctt caatcaagct cttcattccg ttgactgttc
    44821 cgccgccttt catgaaatca tccactacaa ggacacgtga accacgtttc aaacttctct
    44881 tagacagttc catcttttca attctttcag aagatccaga cacatagttg acacttactg
    44941 ttgatccttc tgttattttt gagtcccggc gtacaataac gaatggcgca ttcagataat
    45001 aggacacagc ttgggcaata ggtacgcctt ttgtggctac agtcattaca gcatcgattt
    45061 tttcaccaag gtatttagaa gcaatgattc ttccaacttg acgcagcagt tcaggttctc
    45121 ctaacaaatc agagagataa acatatcctc ctggaagcag acggtcttgt tctgatagac
    45181 gttctgctag agattcaata tattttttcg cctcttcata tgaaatttca gggatgaaca
    45241 atacaccacc tgcagcaccc gggattgttt ccaaggttcc ataccctcgt tctttaaatg
    45301 ttttcttgat gattcccaaa tcttcactga tagaagattt agcagattca taacgttcgg
    45361 caaagcttgt taaggaaatc aattcatgcg ggtgattcaa taaatactgt gtcatgtcaa
    45421 ctagacgttc actgcgacgt actttcacct attccaactc cttttaagaa attttatcgt
    45481 tttacattat ataagttttt cttcaaaaat tcgagcattt tatgaaaaaa taagaaaata
    45541 gttcggtatt tattaaaata aaaagaagcc tataaaaaat ttttcgtttt tataaggctt
    45601 cttttcttta taatttagtt attttttctt ctgaaggcaa ccaatattcg tttgatgata
    45661 tttacaatca ggaacaaagc aataaagact aatgtgattg ttgctcctgg aggtgtatct
    45721 agataaaacg agctagttag tcctgacagc atccctagaa atcctacaca aacactgata
    45781 gcaatcacga cattgaagct tttgcctaat cgcataccga ttgctgcggg aagaaccatg
    45841 attgcggata tcaaaagtgc accggcaatc gggatcatca cagcaatagc cacaccagta
    45901 atcacattga acatcatcga catcaaatga acgggtagcc catccacgtg ggcggtatct
    45961 tcatcaaaag tcagaacata catcggccgt ttgaataaaa agaacaatat accgatggca
    46021 acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac
    46081 agataggatt ggatacttgt tgctgaattc cctccgctca aattcatcaa tactaacgct
    46141 agcgccaaac caccagccat taaaatggca atggagattt ctgaataggt tcggtagatc
    46201 gttcgcagat actccaatat cacagctgct aatacaacga ctagcatggt cgtaagtgta
    46261 ggattaaggt tcaaaaagaa tcctaacgct acccctgcta aagagacatg agaaagcgta
    46321 tcagccatca gcgattgtct tctgatcact aaaaacacac ctagcatcgg ggcgattccg
    46381 gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt catatgaaag caacgccatt
    46441 gagaatcctc ctttctcacc agtcggatct gtctgtccgc atactgtttg atatcttcat
    46501 gatcatgggt gatcattaga actgatttcc catggacatg cgcactatgt cggagaagac
    46561 gataaaattc atttctggat tcttcatcca tacctgttgt tggttcatca agaacaaata
    46621 agtcagggtc agtagcaaaa atacgcgcta aactgattcg ctgcttttgt ccgccagaca
    46681 attcacctat tcgccgattt cgcatatccc acatccctac agcttctaaa gcacgctgta
    46741 catgttgatg atctttttca gagagacgct tgaaccagcg gcctcttgga tatcttcctg
    46801 aacggactaa ttcgatcacc gtactaggaa aacctgcatt gaaagatgca acttgttgag
    46861 gaatataacc gatacttaat ttttttcctt cttgattttt ctcagcaatc tttatcattc
    46921 cttttgaagg tttcagtaat cctaacgatg ctttcaccaa ggttgacttg gctgctccat
    46981 tttctccagt taaaatgaca aattcacccg catctacatg ataagagaca tcttctagta
    47041 caggttcatc atcatagtaa aaggtcaaat cagatatttc gatataacgc accttacaac
    47101 ctccattcta aaacttaatt gatactttct tttaaagctt tcagattttt ttccatgaca
    47161 gaaatataat tttcgcctgc tttgatttgt tcattagtca agctttctaa aggattcaac
    47221 acttctagct tcactcctgt ttcattcgac aatgtctcag ctaccttcga agaggcattt
    47281 tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa attcttttag ctcagctaag
    47341 cgacttggtg tgggttcctg atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg
    47401 tattgattcg ctaaataagc aaaagcagca tgctgagtga caaatgtgcg gtttgttgca
    47461 tcttttagct catcggagta tttttgatcc agtgcttcaa gctttttgat ataagcatcg
    47521 gcattcttcg taaaagtttc ctttttatct ggatattttt cgcctaattc ttcagcaatc
    47581 gttctgactt cttttatagc taataccgga tcgagccaaa catgtggatc gtattcatgt
    47641 gaatgaccat gcccttcttc tgattcttca tgttcgtctg cttcttgcga ttctaaacgg
    47701 tcgattcctt tagctgcttc gatcatcaag gtctgtttgc tatccactga atcctttaac
    47761 gaatccacaa aaaattccat gtctgaacta ttatacacga aaacatctgc atcggaaatt
    47821 tctgctcttt cttttgcact tggttcgaaa tcatgcggct ccgttccagc agggatcaaa
    47881 agtttcacgt ttccttcatc ccctacgatc tcttttgtaa aatcatacat cggataaaaa
    47941 gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt tccacatgct
    48001 gcaaacagta aagcacctat cattaccgta gctgtcaaaa gatatttttt catttatatg
    48061 ctcctttcaa tgaacaaatc ggaattttac gatttattaa cgtgaactaa tgtatcaaaa
    48121 taaaaaaaca cagtcaatag aaatccatca atttctgatt taagcatgta tattcttgat
    48181 taaatcgtaa aatggatgag ttgctgctca tatctagcca tttcactaca ataaaaaact
    48241 gaagaatacc attttaggat tcctcagttt ttcatcacct attttacttc ttatatttta
    48301 ttgcagggtt cgtactaaat aaacttcctc acaaaaacct tttaacccat tatatactct
    48361 ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa
    48421 tgcagcatct gctccgtact tgatcatacg ctctttgatt tgatggatga ctggatgccg
    48481 ctttcccgtt atatcttcta aactgtttcc catttttttg atcatctgct gataatcttg
    48541 ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt tggatggctt gcgtatctac
    48601 ttctctaaac actgtacgtg tagagacact cattcttggt ttgaccaaca caacccagca
    48661 ctgtggcata gaaggtaata cttggatttc ttcccctttg ccaccgacaa atgccgtact
    48721 tccgtagaca cagtaaggaa catctgtacc gacttccaaa ccgatttctg ccagttcttc
    48781 taacgaacag cccagattcc ataattggtt gatccctctt agagcagctg cacagtctgt
    48841 acttcctccg cctaatccag ctgctactgg aatattcttc ttaacaaaga tcttgacacc
    48901 ggttttgata ttgtatttat tttttagcaa taaagcagct tgataaacat tgttccgttc
    48961 atctaccggt aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg ttatttcttc
    49021 aaaaaataga tgatctgcca agtctacgct cgacatcacc atttccaact catgataccc
    49081 atcttgccgt ctatataata catccagtcc taaatttatc ttggctggcg ctttttcaat
    49141 caattccatc ctctcaccca ttcgaaaagt ctaagtcgca agtattctat catagggaaa
    49201 aataaaagta tagcttatgt ctgcctagtg gttgattttg tcatcgtttc aacagctttc
    49261 acagaatttt ctcttcataa tccaaacaag ttctagtgat tcttataatt ctgtgagcac
    49321 ttcttctgcc aaactcggaa catttccttt ttcttttcct atcagactga gtagctgcat
    49381 ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa taacgtcctt cccaatgatc
    49441 gacatatttt aatttctttt cataagcaga ctggaaggaa tagaaaaaat taccataatt
    49501 atatacgata tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc caacattagc
    49561 taatggagct aagcctagat tggcttccga gtagcccatt ttctcgtata caataaacag
    49621 attggctaac agatagtcag ctaattcctc agattcttta ggtaagaccc gcaacaaatc
    49681 atatcccgcc tgcttcttcg tcatcggctg agcggtgatg aagccaacga ctttttgatc
    49741 tttacggaca ataccgacac cgctagataa tacatatgat ttatcaaatc gtcccacaga
    49801 gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa acttcattta cagcttgatg
    49861 taattcttcc ggcagttgat cgtaccaagt aaaatggtat ccttctttca tcaaacgttg
    49921 atattcaagt tgctgactca gccgagacaa gtcttctgtt gctagatcaa caatccctgc
    49981 ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt agctttctga
    50041 tatcttataa aacgcaagct gatagcctaa acaatctgct tgtttcataa actgattcgt
    50101 tgctgcccgc atcattttag gattgccaac tggatctcct aaaacaaagc atttgtttcc
    50161 tttgatctga taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat aaaaataact
    50221 gtaacctgac aagcgaagct gatggcttgt agctgtgcct ccgtatgttt ccaataaacg
    50281 gtcaaaacgg tctttttcgt actcactgcc aagcttcgta gtatcttcag ctaaatattg
    50341 ataaagcgcc actaaagcta gcaatgagat gcccaaacca ataagccctg agaaccacac
    50401 atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc
    50461 gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa
    50521 gattatagct ccccaagaat aaacgaattt tgttcgataa aattcttttc tggataacca
    50581 cacagctaac agaatcaaga catataccaa tatcaactgc caagataacg tacgtgtgat
    50641 tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc agaaatgcct ttttgacttt
    50701 tactgaaagt gctctagcaa ggccgagtaa taaaaagcca accaatagat tcactgtctg
    50761 atctaaaaaa ttgaatgaga acggcaataa tacttgaaat agccgactga ttgcagacag
    50821 attagtgatt gttgatagaa gtaccatcat gatccccgca aaatagacag cacttacgag
    50881 cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat
    50941 tttgactcct gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta gaacataata
    51001 aaacaaccga taatataata gccaaactac tgctacatct tgccgaacag acaattgact
    51061 cattcctaag atcatcaaaa cgtcaaaagt ccccattcca cctgggacca tcgtcagcat
    51121 tcctatcaat gtagcaatca tgaacatagg atagatcgaa agcatcgaaa tatctattct
    51181 catcaacttg ccgatgatca aaaataccgc aagggctccc gtccattgtc caaaggatgc
    51241 cagtagcaga ccaagcaagc cttttggata aaactcgaag aacaatgtct ttctctttag
    51301 ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc agatccaata
    51361 gcttcgaaaa atactttctg actgaataaa aaagacatca ataaatgtaa gaaacgacca
    51421 tacagacaaa ccagtcagca taaatagtgc gacttttgat acagtggcta aaaccatttt
    51481 tctatttgtc tgctttccat aaaaactagc ccgcagactt gctcctacca caccaccaaa
    51541 accggcgaga ttgttgatcg tgtttgttac ccacgcagca agaagaaact cttttctttc
    51601 catttttggt tttccttgtt tttctaagac tctgatcgtc acccaatcat acaacaacat
    51661 cggagtgaca cccagcagtc cagccagaat catgagcagg atcgtcatgc ggctttgttg
    51721 tcccatcctt tgccaaatat cttgccaact catgccatta gcaatatttg cgacttgatt
    51781 tgccacaaag actaacacgg agccgaaaaa aatcagtttt aaaatcagac tatgttcctt
    51841 tatccattgc agcatcaagt atcgtttatt tttcacattt tcccattcct aacttgtata
    51901 cttttgtgtc ataccccaat gttatcttat ttattttaat ttttccactt tagacctgtt
    51961 ctttgcttgc gatgatagaa aaagaaagat atcctaatgc taaaggagag gataacatga
    52021 aagtaggatt tatcggcaca gggaatatgg cacaagctat tattgatggt ttattaaaaa
    52081 aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa aatgcacaaa
    52141 aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat
    52201 cagatatcgt tcttttagcc gtgaaaccaa aaatcattga aaaagtattg gaagaactaa
    52261 gagaatatag ccagaaagtc ttattcattt ctattgcttc aggaaaatca ttaatagatt
    52321 tagaaacagc gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg
    52381 tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga agctgccacg gaggaacagc
    52441 ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt ctatccttta gcagaatcag
    52501 atttcagtac attcattggc cttgctggca gctcacccgc attcgtttac atgctgatcg
    52561 atgcgatggg acggactggt gtactgaatg gtttgtccaa acagacagcc actgagattg
    52621 ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc aagtgataaa aatccatggg
    52681 aacttgtaga ccaagtttct tctcctggcg gtacaactgt tgcaggtgta gttgccttag
    52741 aagaagcagg attcatccca gcaatcatca aaggaatcaa tacgacgatc gagaaagatc
    52801 ttgagatgtt gaataaaaaa taatgatagc aatgtttttt cgtgccttac aaaaaaagaa
    52861 gagctgtggc ttttgccgcg gctcttcttt tgtatttttt attatttagc ataatcagtt
    52921 gcacgagttt cccgaataac gattactttg atgtgtccag gataatccag ttcttcttcg
    52981 attcgtttac gaatatcacg tactaaacga acagcgtcta aatccgagat ttcttctggc
    53041 ttgaccatta cccgaacttc gcgtccagct tgaactgcgt aacttgattc tacaccggcg
    53101 aagctgttcg aaatattttc cagacttctt aaacgacgga tgtagttttc taatgattca
    53161 cttcgcgcac caggtcttgc tgctgagagc gcatctgctg cagctacaag gacagagata
    53221 acagaagtag cttccacatc tccatgatga gaagcgatcg cgttgattac tactgggttt
    53281 tctttgtact tggcagctaa ttccgcaccg atctcaacgt gagatccttc aatctcatgg
    53341 tcaagtgctt tcccaatatc atggagcaac ccggcacgtt ttgctaattg gatatcttcg
    53401 ccaagctctg ctgctaagat accagctaat tttgcaacct cgaccgaatg cttcaagacg
    53461 ttttgtccat aactagttct gaatcgcaga cgtccgatga tcttgattag atctggatga
    53521 agtgtatgag caccgacttc aaacgctgcc tcttccccgt attcgcgaat acgttcatcc
    53581 atttctttac gagatttctc gaccatttct tcgatacgcg ctggatggat tcgtccatct
    53641 tgtatcagtt tttccaaggt catccgtgcg atttctcttc ggattggatc gaatccggaa
    53701 agaacaactg cttccggtgt atcatcgatg attaaatcga taccagtcag cgtctcaagc
    53761 gttcgaatat tacgaccttc tcgtccaatg atacgacctt tcatttcatc attcggaagg
    53821 gtaacgactg aaacagtcgt ttctgaaact tgatctgctg cgcagcgttg aatcgctaat
    53881 gataataagt ttttcgcttt gcgatctgct tcttctttgg cacgctgttc agattctttt
    53941 accattagag ttaactcatg attcagctct tcttcagtcg atttcatgat gacatctcgt
    54001 gcgtcctctt ttgataaacc agcaattttt tctagttctt tttgttgatc ttcaatcatt
    54061 ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt ttgaactaag tgcttcttct
    54121 ttactttcaa gtgagttctc gcgtttttct aacgagtcat ctttacgatt cagcgtttgt
    54181 tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta acttactttc tctcagctca
    54241 ctttcaactt cagaacggta tttctgattt tcttccttag cttctaacaa tgcttctttt
    54301 tttagagttt ctgcttcttt ttttgcacta tcgatgattc cagcagctga tgattgtgca
    54361 ccagctattt ctttctcgtg acgtgacttt gcgaccataa accctaaacc aagaccgaca
    54421 attaaaccga tgatagcgag gagaatgttg aataccataa aatccacctc catactatct
    54481 ttttcatttt tatcatctat acagcttcta tgataaatta ttctaaactc gtcaatatgc
    54541 atcttcgcat atatgtgtac aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt
    54601 agtgtcaact ttaaataaac aaagaaacgg ctttctactt attcattaaa cgatggtttt
    54661 taaaataaat gacactagtt tcatataggt aaacctttaa attgagaaac ttttctatta
    54721 aattattctt tgtagatgaa cagatatttt tattttatgt acccactcgt ataattaaat
    54781 aactcacgat ttctttattc aataaaaaaa cggcaggtca aggagagtac ctactcttca
    54841 gaccagccgt ttttgtttat cataattttt attcttctac taaaggcaat tcttcttgtc
    54901 ctttttcatc ttctgggaca gcgacttctt caccaatacc ataagctgct ctaactagag
    54961 cagaaacttc tgccatcatt tcaggatgat tagccatata gattttagcg ttttcccgtc
    55021 cttgaccaat ccgatcttct ttataagaat accaagcacc gcttttatca acaatgtctt
    55081 tttctactgc catgtctaga agttctcctt cttgtgagat ccctaaacca tacatgacat
    55141 ctacttcagc gattttgaaa ggaggtgcta ctttgttttt tacgactttg atctttgtac
    55201 ggttaccaac aatatctgta ccttgtttca attgttctgc acgacgtact tccaaacgga
    55261 tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat ttctggattc ccaaacatca
    55321 cgcctacttt ttcacggatt tggttgatga aaatagcgat tgtttttgtt ttgttgatcg
    55381 aaccagagag tttacgcaat gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt
    55441 cacccatttc gccgtcgatt tctgctcgtg gaactaaagc agcaactgaa tcgactacta
    55501 cgatatctac agccccactt gatactaaag catcagcgat ctctagacct tgttctcctg
    55561 tgtcaggctg tgaaagaagt agttcatcga tattcacacc taatttttgt gcatattgcg
    55621 gatctaacgc atgctcagca tcaatgaaag cggccgttcc gccatttttt tgtacttctg
    55681 caatagcgtg tagtgcaact gttgttttac ctgaactttc aggtccatat acttcgatga
    55741 tacgtccacg ggggtaaccg ccgacaccta aagcgacatc taacgcaaga gagccacttg
    55801 gaattgtaga gatttgttgg tcgatttttt cgcccaattt cataatagag cctttaccat
    55861 agctcttttc aatttttttc agtgcagcat ctaaggctgc tttacgatca tctgccaata
    55921 cgataatcct ccttatattt acaaatcaga gcaacatttc attatcctta tcatatcttt
    55981 tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg catttatcat tttcttaaaa
    56041 gctgttttct caaaagatca aatccttgca taaccgcatt atatctgata gcagcacgat
    56101 ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt agatgaaata gcgatccata
    56161 ctgttcctgc agattgacct tctaacgtgt ccggtccagc cactccggtg aaagctacac
    56221 tgtaatctgt tcttgctatc tcacgtgctt gaatggccat ctgctccaca cattctttgc
    56281 tgactgttcc atattctgct aatagctccg gatcaatctc cagaaaacca gcttttgttt
    56341 ggagcgagta ggtaacgaat ccgcctggaa atacttcaga tacaccagca atgtcgccta
    56401 atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt
    56461 ctttcagtag ttccactacg acttttgcta gcgaattatc atcgccgtat ccataaaaat
    56521 attcccctac tcgttcttgg attttttctt ctaatgctaa aagagcttga ttccctgcct
    56581 gaacatcatt tgttttcact gtcagtcgta atgtcacttc atttggtttg gcatatggtg
    56641 cgatcgttgg attgatctgt gtctcaatca gatcttttag ctcagtgacc agtcgcgatt
    56701 caccaatccc ataaaagcgc aaaactttag aaattagttt ttcttcagaa ggaaaacgtt
    56761 cctctagtaa aggacgtact tgttccacaa acattggttt caactcatta ggcggaccgg
    56821 gaagaaggat atacgcatgt gtatctgttt gataaaaagt ccctaaagca agccctgtac
    56881 gatttggtag aggaaccccg ccttcgataa tctgggactg ttgcagattg tttttggtca
    56941 tttttcgatg ggttgtttca aaatatgcca ataatttttt gtatccttcc gtattttgta
    57001 tgagagactt accgaggtga gccgcagttg cttcttttgt caggtcatct tctgtcggtc
    57061 ctaatccgcc acataagagt atgagttcac ttcttgtttc agctagagca atcaactctt
    57121 ctaaacgctg ctgattatct cctacaactg tttgataata aacttcgata cctagatcag
    57181 ctagctgttc tgacaagaaa gtggcattcg tattcacgac ttgtcctagt aaaagttctg
    57241 ttccgacagc tattatttct gctttcatta cttcccgctc ccttatatag tatacgctgt
    57301 atttttatat ttttttattt aaaaagaggg caaggagctg tgacaaaaat cttgtcacag
    57361 ctccttgttc caaataaaca gtgttctcct gccaaaacct cggtcataag tcacaatata
    57421 cataaaacat gaaaagctgt tttttgaaat tatcccttgt gactcagagt tagacggcag
    57481 gctcacaacc tctccactaa acgatactta cattgatcct ttgaacacac cgctgttttt
    57541 gatgaagtaa tccacacctg aatagatagt aaagatcaga caagcataaa gcatgatttg
    57601 atctactgga ataccgatca ggctgaatgg gatattattg ataaacagca agataatagc
    57661 aaccatttgt gtagccgtct tgacttttcc cggccaagct gcagccatga cttctccatg
    57721 ttcgacaagt agtaaacgca agcctgtgac ggctaattca cgacagacaa tgatcgctac
    57781 tacccaacca ggggcctttc cttggtcaac caacatgata aaagcagtca taactagcat
    57841 tttatctgcc aacgggtccg caaacttgcc aaaattagtg acaaggcctc gcgcacgggc
    57901 aatttttcca tctaaccagt ctgtgatact agctaaagca aaaataactg cacctactag
    57961 ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt ccccaatcta aaggtaaacc
    58021 tacaaccaaa atgaaaatcg ggatcataca aattctaagt acagttaatt tatttggtaa
    58081 attcacacta tcgactcctt tcttccctat tctacgtgac tatcagcgaa gtttccaccg
    58141 atattattta cttttccaaa tgaatttatt caaaggagta tttttattga aattgatctt
    58201 tttgaattaa cgaagaagga ccgcgacaca gatttgtcac gatccttctt ttttatacct
    58261 acaagtaaac agatgcgtaa actaatttat acgatcattc tgcgtattga agagtaaggt
    58321 tcacattttt tggactctga ttgttttccc ctggattaac tggtacggat tctccattcg
    58381 ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg
    58441 gtaacacggt actctgtgtt tcatttgctg ccagtgtccc ttgataaaca taggcattgt
    58501 ctactagcac acctaaccat acacgattga cagcagtaaa ttcaagggtt acaggcttgg
    58561 tagcgtctgt gatatttact gcgatggcac tgccagaatc ttctcctgta gctattgcca
    58621 ttttcttttc ttcacttgat tttgttgttg attctactgt agaagactct ttggtctgcg
    58681 tgctttgctc tgaggaagag gactcctcga ctgatccttc tactttaagc gaagaagctg
    58741 tgtctccgat tattggatca gcttgacgat cttgccaagt catatagccc acgacaacga
    58801 caatcgccaa agcaaccaat cctaataaaa tgacaggaag acttgtccaa aagcgacttg
    58861 gatgcgattc ttcctgatgc attgcttttc tagaagtact tactgtttca ggctgcggac
    58921 gttcagggac cgatcctgtc aatttgcttt tcccatcaaa aacatcgact aaatggtctc
    58981 catcttctcc tacagcttca gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag
    59041 gcaattggtc aaaatcatca ttgtcgattg cttccaaata acgtttttga atttttgtaa
    59101 tctgctgtaa ttcatctaaa gatatttttt tattcaggcg tgcttgccgt aatttttctc
    59161 cgatggtttc aaaagccact cgttcatctt ctccagtctg tttgattctc cgaagcatct
    59221 gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca aaagtcatgt cacagctcct
    59281 tttttccgaa taaacattgt tctcctgcca actcctcggt cacaagtcac aatatccaat
    59341 aaatataaga agctgttttt tgaacttgtt ccttgtgact cggagttaaa cggcaggctc
    59401 acgacctctt tcttaaacgg tgttcaatca gctgttcctc ggtattagct caaaaaccgg
    59461 caaaatataa ggagatattt ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt
    59521 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agtccaacca
    59581 agttaaaaac attaagaagc tgtttttact tggctgttct tattacttga tgcagggcag
    59641 ctttttcaca acctcttggt acacggtgtt ccagaagtag cttcttcggc aataagccga
    59701 aatatcacaa aaatttgaga agcaattttc gtaaattcc
//
LOCUS       DAHHPA010000006        56278 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.006, whole genome shotgun sequence.
ACCESSION   DAHHPA010000006 DAHHPA010000000
VERSION     DAHHPA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.006"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(41..1609)
                     /locus_tag="NBH55_000327"
     CDS             complement(41..1609)
                     /locus_tag="NBH55_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCD1508343.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1613..2023)
                     /locus_tag="NBH55_000328"
     CDS             complement(1613..2023)
                     /locus_tag="NBH55_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD1508344.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2020..2943)
                     /locus_tag="NBH55_000329"
     CDS             complement(2020..2943)
                     /locus_tag="NBH55_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCD1508345.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2940..3818)
                     /locus_tag="NBH55_000330"
     CDS             complement(2940..3818)
                     /locus_tag="NBH55_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCD1508346.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3805..5142)
                     /locus_tag="NBH55_000331"
     CDS             complement(3805..5142)
                     /locus_tag="NBH55_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCD1508347.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5145..6248)
                     /locus_tag="NBH55_000332"
     CDS             complement(5145..6248)
                     /locus_tag="NBH55_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCD1508348.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6322..7830)
                     /locus_tag="NBH55_000333"
     CDS             complement(6322..7830)
                     /locus_tag="NBH55_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD1508349.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7796..8488)
                     /locus_tag="NBH55_000334"
     CDS             complement(7796..8488)
                     /locus_tag="NBH55_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCD1508350.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8510..9484)
                     /locus_tag="NBH55_000335"
     CDS             complement(8510..9484)
                     /locus_tag="NBH55_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1508351.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9784..10656
                     /locus_tag="NBH55_000336"
     CDS             9784..10656
                     /locus_tag="NBH55_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD1508352.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11114..13312)
                     /locus_tag="NBH55_000337"
     CDS             complement(11114..13312)
                     /locus_tag="NBH55_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508353.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13544..14536
                     /locus_tag="NBH55_000338"
     CDS             13544..14536
                     /locus_tag="NBH55_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1508354.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14596..16017
                     /locus_tag="NBH55_000339"
     CDS             14596..16017
                     /locus_tag="NBH55_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCD1508355.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16010..17509
                     /locus_tag="NBH55_000340"
     CDS             16010..17509
                     /locus_tag="NBH55_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCD1508356.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKYPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17548..18942
                     /gene="uxaC"
                     /locus_tag="NBH55_000341"
     CDS             17548..18942
                     /gene="uxaC"
                     /locus_tag="NBH55_000341"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCD1508357.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19101..19784
                     /locus_tag="NBH55_000342"
     CDS             19101..19784
                     /locus_tag="NBH55_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCD1508358.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19786..20622
                     /locus_tag="NBH55_000343"
     CDS             19786..20622
                     /locus_tag="NBH55_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD1508359.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20633..22387
                     /locus_tag="NBH55_000344"
     CDS             20633..22387
                     /locus_tag="NBH55_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1508360.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22663..23004)
                     /locus_tag="NBH55_000345"
     CDS             complement(22663..23004)
                     /locus_tag="NBH55_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCD1508361.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23072..25243)
                     /locus_tag="NBH55_000346"
     CDS             complement(23072..25243)
                     /locus_tag="NBH55_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCD1508362.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25412..26311
                     /locus_tag="NBH55_000347"
     CDS             25412..26311
                     /locus_tag="NBH55_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1508363.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(26382..27239)
                     /locus_tag="NBH55_000348"
     CDS             complement(26382..27239)
                     /locus_tag="NBH55_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD1508364.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27224..29926)
                     /locus_tag="NBH55_000349"
     CDS             complement(27224..29926)
                     /locus_tag="NBH55_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCD1508365.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29939..31228)
                     /locus_tag="NBH55_000350"
     CDS             complement(29939..31228)
                     /locus_tag="NBH55_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCD1508366.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31378..32424
                     /locus_tag="NBH55_000351"
     CDS             31378..32424
                     /locus_tag="NBH55_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1508367.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32426..34579
                     /locus_tag="NBH55_000352"
     CDS             32426..34579
                     /locus_tag="NBH55_000352"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCD1508368.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(35052..36503)
                     /locus_tag="NBH55_000353"
     CDS             complement(35052..36503)
                     /locus_tag="NBH55_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1508369.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36500..38224)
                     /locus_tag="NBH55_000354"
     CDS             complement(36500..38224)
                     /locus_tag="NBH55_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1508370.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38217..38831)
                     /locus_tag="NBH55_000355"
     CDS             complement(38217..38831)
                     /locus_tag="NBH55_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCD1508371.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(39176..40633)
                     /locus_tag="NBH55_000356"
     CDS             complement(39176..40633)
                     /locus_tag="NBH55_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1508372.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(40674..41594)
                     /locus_tag="NBH55_000357"
     CDS             complement(40674..41594)
                     /locus_tag="NBH55_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD1508373.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(41606..42553)
                     /locus_tag="NBH55_000358"
     CDS             complement(41606..42553)
                     /locus_tag="NBH55_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1508374.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(43032..43751)
                     /locus_tag="NBH55_000359"
     CDS             complement(43032..43751)
                     /locus_tag="NBH55_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD1508375.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(43800..45446)
                     /locus_tag="NBH55_000360"
     CDS             complement(43800..45446)
                     /locus_tag="NBH55_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD1508376.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            45625..46215
                     /locus_tag="NBH55_000361"
     CDS             45625..46215
                     /locus_tag="NBH55_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD1508377.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            46307..47812
                     /locus_tag="NBH55_000362"
     CDS             46307..47812
                     /locus_tag="NBH55_000362"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCD1508378.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(47896..49248)
                     /gene="celB"
                     /locus_tag="NBH55_000363"
     CDS             complement(47896..49248)
                     /gene="celB"
                     /locus_tag="NBH55_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCD1508379.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(49248..49766)
                     /locus_tag="NBH55_000364"
     CDS             complement(49248..49766)
                     /locus_tag="NBH55_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508380.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(49782..50108)
                     /locus_tag="NBH55_000365"
     CDS             complement(49782..50108)
                     /locus_tag="NBH55_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCD1508381.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(50121..52100)
                     /locus_tag="NBH55_000366"
     CDS             complement(50121..52100)
                     /locus_tag="NBH55_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1508382.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(52121..52435)
                     /locus_tag="NBH55_000367"
     CDS             complement(52121..52435)
                     /locus_tag="NBH55_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCD1508383.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            52603..52896
                     /locus_tag="NBH55_000368"
     CDS             52603..52896
                     /locus_tag="NBH55_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCD1508384.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(52974..54086)
                     /locus_tag="NBH55_000369"
     CDS             complement(52974..54086)
                     /locus_tag="NBH55_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD1508385.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            54473..54652
                     /locus_tag="NBH55_000370"
     CDS             54473..54652
                     /locus_tag="NBH55_000370"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508386.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            54846..55016
                     /locus_tag="NBH55_000371"
     CDS             54846..55016
                     /locus_tag="NBH55_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1508387.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(55440..>56278)
                     /locus_tag="NBH55_000372"
     CDS             complement(55440..>56278)
                     /locus_tag="NBH55_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1508388.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT        16771 a        11511 c         9918 g        18078 t
ORIGIN      
        1 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
       61 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
      121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
      181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
      241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
      301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
      361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
      421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
      481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
      541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
      601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
      661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
      721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
      781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
      841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
      901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
      961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     1021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     1081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     1141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     1201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     1261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     1321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     1381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     1441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     1501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     1561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     1621 cttttctcat tcttactcga tataaactga atgccccagc tgcgaaataa aaaaatgcaa
     1681 aaggaaacgc aataaactga gtgccaatca acaatatgaa tcctgttata aaatacaaac
     1741 cagtggcagt tgttaagtat tttcctttag cagtcaaaaa caatgctact gctaaaaaag
     1801 caagtgaaat aaacaacaca acggcaaaca gaccagtcat gtcctgtaat aatccaaatg
     1861 tattttctaa agacaaagtt gaatgatcta cagcaagcga ctgataactt tttttaaact
     1921 ctgacgcaga catattactt aaagtaactg ctaacccgcc aaagaaaatc aaagatagac
     1981 tgccaccgac cattcctagc aatagttccc attttctttt catttctgaa ccccctgctt
     2041 ttctaaatgt atccattcag gtcttttgat cgatgtttca taattgttat tacgttcaga
     2101 gaatttgaca gtctgttcat ttttcttgtc atcccaatta tcccaaccag ttgggtgaac
     2161 atgcccacct atcttacacg cttcaaataa gacatttgca tacggacgcc acggtcttcc
     2221 taaatagacg tttctagtat caggttctgc agtcaaggta cagctttcaa aacgataccc
     2281 tgttttttga tttttcggcg tagatgctgc tgtgatgtat ccaggatttt tactggctaa
     2341 gctatggata tgacactgat gaaaaacggc atctgcccca ccaaatatga aatccaccgt
     2401 tccttcaatc cagcaatatt cataggtctg acgacaatac tcaggattgg cagaagccgg
     2461 tgtacaaaaa ggagtaccat ctttttgcaa atcaggcaac ggacccgtgc ataaggtatc
     2521 ctgataacca gatagtcgac agttttttaa agatacgcga tctcctgaat tgaataaagc
     2581 aacagcctgt cctacctttt ctcctggacc tgctgtattt tcgatgtgaa gattttctaa
     2641 tacgacattc gatccttcta agaaaaaagt agcacttcgg aaagtcccta actggctacc
     2701 gtcaagatac tcctgttttg caaaacgatt accggagata actactgttc ctgctcccac
     2761 aagtttaaaa ttggataatc tgctttctat atattcatca tatcttcctt cttttactat
     2821 caagaccttt ggatggctat ccggcaggcc tgccaaataa tcgattcctt cttggatagt
     2881 ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt atcagtttgt ctttcatcct
     2941 cataaatgct cccttatgaa ttgtttccaa atatttctca tttcttgtgt ttccatatga
     3001 ccgcctgtta aagaaaaaga agaaaagttt tctggactac ccattgattg atactttttg
     3061 tccagccctt ttctcagctt cagtacgcct tcgtccaagc acatcgtatc gttttttcca
     3121 actaagctaa gacgagggcg cggagcgatt ttttcttgga ttgctaacgt tgtataagct
     3181 gtcaatagat ttggcacata gtaataaaac ccatgatgat ctaggcggcg atgtagcaaa
     3241 agtatttcga tgtccacctg tgccgctaga tcaatacaaa ctttgacacg agaatccaat
     3301 gctgtcaccc accagcttaa taagcctccc atagaaagac caatcgttgc gagccgttga
     3361 gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc tgatcacatc ataaatacgc
     3421 attccccata acgtttttcc tgttaataaa aattgtttga acaactcgct ttcagaaata
     3481 cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa cagcatatcc cattcctgtc
     3541 agtacttctg caaaagatgg agaagctaag taaggagcac catggatcaa ttcacttttc
     3601 ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga ccggcggaaa cggtccttct
     3661 ttttctggat aagcatagag ggctggtact gtttccaatt catttaagta caattgatag
     3721 tattcaagca taaaaccgtt ttgtttttct cggtataatc gttttccact aggggtatgt
     3781 ctgctagatg gagcacctaa aaaatcattc aactgcataa gcaaagtttt tacgaacaac
     3841 cagttcatcc atattttctg attgatttga ttggaatttc tcaatactga tttctttagt
     3901 attttctaat agaaatgctg gtccctcgtg attttcaatc gacatctggt taaaggaaac
     3961 cttacccgca aagccaatat aaaatccttg tctgctcatt tcctctattc ctgtcatcat
     4021 agcaggaaca ccgggaactg catcttgggc catcgagaca tcgatattgt caaatgtgat
     4081 ttcttgtatg aattgttccg ctaatccata aataaaacct gcagaagcat ggacattcct
     4141 tgccgtgata ttcgaaaaat ggatccttct gaatgctggt gtccgttcat ctatcggata
     4201 agcctttttt tcccatacat acggttcttt ccctcgcgga ccacagaaat aataaagatt
     4261 cagtataaat ggacacatca cattatccat gactatattg gatactcgaa tatcttcaac
     4321 gatcccgcct cgacctcgtc tagatttcaa tcgtattcct cgatcagttt cttggaagat
     4381 acaatttgaa atggtaatat tacgaatact accactcatt tcacttccta atacgacacc
     4441 gccatgtcca tggaccatcg tgcaatttgt gatcgtgata ttttcgcaag cgatcctttc
     4501 gtacgtatct tcagtccctg ccttgattgc aatacagtca tctcccacgt caatatggca
     4561 attactaata cgcacatttt tgcatgattc tggatcaatt ccgtctgtat ttggtgaatc
     4621 cgctgggttc aagatcgtca agttatcaaa cgttgcatta ctgcaaagaa tcgggttgat
     4681 cgtccaactt ggtgactgga tcaatttaat atctttcaca gtaattcgat gacagttatg
     4741 gaaactcatc aatttaggac gaggataagc gagattatct ggttcatttc taaaagtatg
     4801 ccaccatttt tttccatttc catctaatgt accgaaacca gtgacagaga tgttctctac
     4861 attttgagca tagatacagg aagcataaac ttttcgatgg actccttccc aacgtgaatg
     4921 aacaacagga taatcttttg gatcgtcaga gaatttcaga accgctcctg cgcttaaatg
     4981 aagttcgaca ttgcttttta aaaataaagc tcctgttaga aattcacctg ctggaacaac
     5041 tacggtcccg cctccgtcac ttgctgcagc atcaatggct tgttgtattg cttcagtatt
     5101 caattcatca attgatgcgc caaatgtcaa aatatcatac atatttataa tgcctccact
     5161 tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat cattacaaat aattggttca
     5221 ctgatgtagt aagcatatga accatctcgc tgatctgctc cgcctaaccc tgctacttga
     5281 cagtttttat tcaagttcag ccagccttct tttgtttcca gaacaaattc atcaagtaat
     5341 ccttgatgtg atttcttcac ttgtggaagc cattcttctt tgttcagcag tcccaaacgt
     5401 attcctttag ccattgcgta aacaaacata ctactgccgg aagcttccag ataatttcct
     5461 ttccgttttg tttctgttgt tacttgatac catgtcccgc tgttttcttc ttgatatgga
     5521 agtaatgcag ccagtgtctt ttctagaatt tctttcagca aagaacaatc tgtttcttca
     5581 tgaagtaatt cataggtatc tgctgcagcc atcaaatacc agccaatcga tcggctccaa
     5641 aaatgatggg atagacctgt ttttggatca gcccatggtt ggaccttctt ttcatcccat
     5701 gcatgaatca gcaaatttgt ttttggatcg aatagatgtt tataactgat ttcaaactgt
     5761 ctcaaaacat cttggtaatc tttttcttca ctaaaattct ggataaattc agcataaaaa
     5821 ggagaaccca tatataagcc atccaaccaa atttgataag gataaatttc tttatgccaa
     5881 aaagctcctt cggatgtgcg tggatggttt gccaactgct gggaaaggag ttctgctgct
     5941 tttttatatt tttcttcgcc tgtttctttg tacaataaaa agaaaagctt tcctgtattt
     6001 acatggtcga tattgtattc ttcaacgctg tagccacgga tcgttccatc ttcttgcacg
     6061 aaatagtcga tattgtcttt tatgaaatca aaataaattt tttcttgggt tcgttcccaa
     6121 agtaattgga accctttaag tacgacgcca tagtcataag accattttcc tttataccac
     6181 ttttcttcat ataagcgagg tgttctttcc atgatacttt ttgctaattt ctttgaccag
     6241 ttttccatta tttgctctcc tttaaaaatt aaaataggat ttgggacaaa taccaagaag
     6301 tatttgtccc aatcgtctat attactgtgc cgctgctttg tatgcttcct cgtactcttg
     6361 tgtgattcga tcgcctcctt gtgctttcca gtcttctaca gcttttttga atccatcttc
     6421 gtcaagctgt cccatgatgt attgcaccgt agcatcaatg acgatttttt caatttctga
     6481 tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa ggatccatta cagcaaattc
     6541 ttcattttct ttgatcaatt gattagagaa ttctttttca gggtctgtat ctttgaaaga
     6601 gaacgtcact tcactaggac gagagcttga taacggttgc acctcttgtt gccaaagatc
     6661 catatcgata aattcgatag aaccatcatc attcaattca taatgcgttc cttcgatacc
     6721 gccagtcatc aatttataca catcttcttc taaaagatca tcaacaaatt gaagtaattt
     6781 cttcaattgt gcttcgtctt ttacttgaga tttgggaaat gctagcaagc cgccaatacc
     6841 atttccttct gaccaaacat gataaccgcc gccttcacca tctgagatct tgtttacagg
     6901 cactaattcc atatcatctt gaataccttg acccatcgtc ttcagattct tgatatccac
     6961 cattccagtg tagattccag ttcttccttg tgcaaattgt tcttgctgat cagtcttttg
     7021 ggtaactgca aagtcttgtg ccaaatagcc attctcaaat agatctcttg aaaaattcaa
     7081 agctttcata tactcttctg tatcaaattc tggagtgaat ttgccattat cgtctacagc
     7141 ccagccattt ggtgctccaa aataagaagt gaaaagttta aagctggtat aacgaatatc
     7201 actaggcgca cgatcgccaa aaccaactgt gtcgtccaca ccatttccat caggatcgtt
     7261 ttctgtaaat tgtttagcta cttcataaag ctcatccatg gtagttggaa catctagtcc
     7321 tagattatcc aaccagtctt ttcgaatgac taagccagca cgagcgtagt ctttttgtat
     7381 cggtacgccg tacaaggcac cgtcgatact cgcagcttcg attctatctt cagaaatttg
     7441 acttaaattt ttatagtctt ttaaatattc tgatacatcc cagaatacgc ctgattttaa
     7501 cgcattgcgg acagaagagt ttttcaacat cgcaatttga agtgtaacga tatctcctag
     7561 ttcatcagaa gccaatgcag ttgttaatct ttcttctttt gaagcatcag gaatccagtt
     7621 gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc acaatcgtgt ctgtaggcgg
     7681 tgaagcatga tgcaaaatat ttaaccaagt gattttagct tttccatctt ctgtctctga
     7741 agaactgtta cttgaactgc tgccgcatgc ggcaagtgct agaattcctg caaaactaag
     7801 acctaatttt acgagcgttg cttttttcat aaattttctc cttcactgtt ttaataatat
     7861 ttttgttttc ttctgttttt tctgtatatg agttcttcca ttccaaatgg caaaatatag
     7921 aatttgctgt ccacgtgact gcggaaaaga agagactgcc accgataaag gttaaaacac
     7981 ctggcattgt ttggatgatc atcagataaa tccctaccat acttaatatc aacactaatg
     8041 agtaatgaag atgtgagaat ccgaataaaa atgccatttt cattttgaaa aaatatcctt
     8101 tccaatcata atgagccatg attgggaaaa tatagagtgc tgagagcaca tatatgaacg
     8161 cagcaatata caaagcgatc ctaagatacc aattgtgtat gaaagaaaga tccacaagta
     8221 aaacaatccc gatggcgcca tatatccagc tcatcagcaa ggtttcacga tagttttctt
     8281 taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc ttttgagcgc agccattttc
     8341 taatcactgc aaccatcgca tacgatgcag gaccgaaagt aaaaactccc aaacctgcga
     8401 tgactaaacc gccccacaga aaattcaaat aaaccaactg taaaatacga tttagccttc
     8461 cgtttatttt atctaatgac tgcagcatat ctttccctct ctttctatct tagtaaacgc
     8521 cgtcttctcc tagctttttg gcaatcttat tagctaaaat taccataaac aatcctacga
     8581 cccctttaaa caatcccatt gctgtactga aacttaactg tccattcttc aatccagctg
     8641 tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt caatgaattc aataacaagt
     8701 acatatgctc gaaccctaag tctaatgtct gaccgatttt tagaatcagc aaggtaatga
     8761 ttactggacg aattgccggc aatgtaacat gccatgtttt gcgtaagcgt cctgctccat
     8821 ccatttctgc agcttcatac aactgtgtat cgactgcagt tatcgctgaa aggtagatga
     8881 tcgtagacca gcccaactct ttccagatca cttgtccgat atatactgtg cgtgtccatt
     8941 caggggatgt tagaaaactg attttttcaa atcccattgt tgcaaggaat tcattcaata
     9001 cccctccgtc aacattcaaa aatacataag taatcgaaac aatgattacc caagacatga
     9061 agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa tcgtttgttc gttacttcat
     9121 ttaatagtaa agacaaaatg attggcatcg gaaaaaacac gaagatattt aatccgaaca
     9181 agatcaacgt atttcttaat aatgtaaaga atgtcggttc tgtaaacaaa cgaatgaaat
     9241 gtttgaaacc gacccaagga cttcccatta ttcctaaaaa tggttgataa tcttgaaaag
     9301 caataacgag tccccccata ggaagatacc taaaaatcaa gaaataaagg actcccggca
     9361 gaatcatcac atataaaaat ttattcgttt tgattcctgc aattattttt tttcgtcttc
     9421 tgagcttttc ttcttttttc gatagcgtca gttgaaatgg ttcttgtttc tttacacttt
     9481 ccataagccc ctccttagtt ttgtgaatgt ttgctgctta tgacttcatg atagataact
     9541 atctgaatta cgtatataat catttttacc gtaagtaaaa acgcttacat agcaatgatt
     9601 tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa taaactacgc atagcaaacc
     9661 ttctattttt ttctatctga cacttagcat aggaaatgat tatatacgta atctttcacc
     9721 gatgataaat tgaacatata gaaacagaaa catttaattt attttgagga ggagcctatc
     9781 tatatgaaaa agaaagtctc tataggagaa agaagtttta cacttttcaa caatacgttt
     9841 ctaattttgc tagctttgat ctgtatcgtt ccttttttaa atatcatcgc tacatctttt
     9901 gcttctacac aagaagtcgt cgcgaaaaag tttatccttt ttcctactac cttttcttta
     9961 gacgcgtatc gctacattct ttctacgccg accattttta gagcacttgc tgtttcgatt
    10021 ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa cttcactgat ggcctacggt
    10081 ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact ttcttgttgt attttctatg
    10141 ttgttcagcg gggggatgat tccaacgttc ttagttgttc gttctctagg gctggtcaat
    10201 tcttactggg ccatgatcct gcctgtggct gttaatgcaa tgaatatgat cattatgaga
    10261 aactttttcc aagccttgcc ggatagtttg gaagaatcag caaaaatgga tggttgcaca
    10321 gattttggtg tatttttcaa aattatgctg ccattagcgt taccgtctat tgctactatc
    10381 tcgctttttt atgctgttac ttactggaac acttatatga ctgctatttt gtatatcaac
    10441 gattcatcta aatggccgat acagattctt ttacgccaaa tcgtcattgt atcaagtggg
    10501 atgcaagcag aaagtagtgc cgttgatgtg attccaccag cacaaacagt taaaatggct
    10561 gttatcgtta ttgcaacagt gcctatgctg attgtttatc cttttgttca gaaatatttt
    10621 gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat gtactgaacg caaaatggta
    10681 gttgtgtcag aagtggtcag cttcaagaaa taagaaggca tttacgaaaa ttgcttctta
    10741 aatttttgtg aaatttcggc ttatttttcg aagaatctac tcctggaaca ccgtgtatca
    10801 gaggttgtga aagagacgtt cagccttgag cgatgataaa aactctcaca aacattgcga
    10861 cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa tagcttccca tatttcacaa
    10921 aattatgcct tattgtcgat agcctgtctc ttgaacgcca tttattcgta aaaaagaact
    10981 gtgacaagac ttttgtcaca gttctttttg cgtgcttagc cttgaaattt aactatccat
    11041 aaatacaaat actagggtta ttctaacttc ttttattgct tgaatcagtc cacaagaaaa
    11101 acagcctctt gattcacaaa tatttttttc gatattctcc cggcgtaatc gattccttct
    11161 ttttaaagaa acgaataaaa ttttgcggat ttcgatattg caatctttct gcaatctctt
    11221 ttacggagag gttagtctct ttcaaccatt tttttgctac ctctaaacga tgattttgaa
    11281 caaaatcacc aaatttttct cctgtttcct ttttaaatac attacttaaa tagttagggt
    11341 tataatgcaa cctatcagcg atcacatcta aagacagatc acgatcatat tctgtgtaga
    11401 cgatatgcat aatattctcc gacaaatttt taaattcatg atcagtgatt gcttgagagc
    11461 attctgtgat tggcagaact aattgttcta aaacaagttt ttctagttct ttgggatgat
    11521 aattgttcag tgcttttaca taaatttgtt tcatgttatc aaagtttttt gaatctactc
    11581 ccaataactg tcctaattga acgagttcat tgatcaagcg gaccgaaact acttcccgac
    11641 tcaaaggatt tttattttgt gcaaacaatg catcaatcaa ttcatgtact aaattcttca
    11701 cttcctgatt tccagaacga atggcttcaa acaatcgatt ttgttcttct atcggatatc
    11761 taataagtgt tttttcatga gcagcaggaa cgatttcctg ataaaaaata attgaacttg
    11821 gaccagtatt tacacgataa tacagggctt cttttgcccg atcaaccgat tgcttgcttt
    11881 cattcagcgt ttcgaaccgg ttactgatgc ctatactgat agttaaattc aagtattctt
    11941 ttacacgttt ttgaatgagt cggcaatact ccatgacttt tttgcttgct tctgcatcat
    12001 ctttatccat tcgataaata gtggcttgca tttcttcatt caaaacgatc ggcagcattc
    12061 gattttcctt tggcacaatt tccgtgatca tgttgttgat cgctaaaagt agcaaatcac
    12121 gctccccacc gtgactatct tctaacagat cgatttgaac caaagctgta taatagcagt
    12181 cattttgaga actataacca aattgctgca accgctgatc tagttctcgc ctttctactc
    12241 gatttctaaa taaactaagt acaaatagcg tttccagctg tggcttttgg gtaactaagt
    12301 gtgcacttaa cgcttctttt tctcctatga tccgactgac tgattgagcg accaaatcta
    12361 gttctttccc actgaatcct tttgatttaa gattcagacg ttcctgtatt tgagaaatcg
    12421 gacgggcaaa tctgtccgaa aataagtagg aaagaagacc aatgagtcct accagtgtta
    12481 aagacactgc aactagcccg attctcagat tgcggatcat tgcgccaatt gcactagagt
    12541 caacttctaa tctgtaaatc caattattat aatctgattt cagtactata tacgttttat
    12601 ctttttctct tatcactttc agctgattgt aatctagatc cacgtccgtt aatacagttg
    12661 gttgctcttt attttttact gtctgagtag taaaaaggtt gccaacttcg ttttcaatag
    12721 aaagtatgtc ttctccactt tcaccaacaa cttgttctat tgtatgtttc tctatcgtag
    12781 caattcccaa tgcatatttt tctttttcaa acattggcaa agaaatcacc atttcgattc
    12841 ctttgttttt cttttgccaa aataaattat ttttgttatt tatgtacttc tgttgatact
    12901 tttcaatttc ctcttcggtc agttgactca acgatccatt gattactccc catttaccag
    12961 ccaaactgac taaatcataa ttattatcat ccataacaat cgtttctata aaatttagtt
    13021 ctttcctgat ttcagaataa gtggagtaat ctgtatatgt cagcggatgc ttgaatatgt
    13081 ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata ttcaatcgtt ctaattttcc
    13141 attcaacgtt agttttaact tgcctgagca aattcttttt gtctgttaaa taactcgatt
    13201 cgatcgattc ataggtattc tggtaaatga ataaactaaa tcctatgaca agtacggctc
    13261 ctaataaaaa catcggtaaa aatattttat aaaatactgt acttttcttc attttcttcc
    13321 tctcctcctt ttaaaataaa aggctttcat ttttctgtaa aattgatact catctaatta
    13381 aatgagcatt ccccgctttc aagaatagca taattaaaaa taaaaatcca agtaattttt
    13441 aacgtgtgca atcttttcat atcaaagatc tattgactta tcgtagttat cgtaatatga
    13501 tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa cgatcaaaga
    13561 tgtcgctaaa gcagctggcg tttctcatac gacagtttct cgtgctttga ataacagcag
    13621 tttaataaaa aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa tgaattattc
    13681 gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg gactattttt
    13741 ctcaagtttg cataaaggaa cttcatcaaa tttcctagca gattccattg caggcatcca
    13801 ttcagtatta gacacaaatt acagtttatc tgttgaaagt atcgatgttg ttgatttaac
    13861 agaaatcaac ctgcaacgct acgatggact gatcgttatg agtcaaagtg atgatgatca
    13921 gcctttcatc gactatctca aagaaaaaga atttcctttc gttgttgtca atcgtcacat
    13981 caatgatcca gcggttatca acgtcactgc cgatgatgcg gccggtgtta tgcaggcagt
    14041 agattacggt attggatgcg gacatacgaa gatcgcttat attggcggaa agaaaaattt
    14101 tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag cgagcatccc
    14161 tgtcaatcca gcctattttt taacaggaga ttacagctta gaaagcggcc tccaaaacat
    14221 ggaataccta ttatcacttt ctgacctgcc tactctggtt ttttgcggca acgacgacat
    14281 agcgattggt gcattgagag ctgtacattt agctggcctc cgtgttcccc agaacatttc
    14341 tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc ttaccaccgt
    14401 acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag atttgatcga
    14461 aggcaggacg atcgaacaat ctaaaattga gctctccacc ttgttgatcc ttcgtgagtc
    14521 gatcaattac ttgtaaacta caagtaattt tatataaaaa tgttaacgtg tgcataacac
    14581 agaaaggaag tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa cgaagcacct
    14641 gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg gatttattga ttggcagatt
    14701 cagcagctga ataaccaaca attgtttaaa ggtaatgttg caatcattca acctttagaa
    14761 caaggtcttg gaaatatgat gaaccagcaa gataatttat atacggtcat tttacaagga
    14821 ctcagcaatg gcgaaatcgt tgatacttcc gagattatca ccgtagtaga acaaatgatc
    14881 aatccttatg atgaatggga tgcttatcta tctctagcag aaaatgacga cttggcctat
    14941 atcgtctcca atacaacaga ggcaggaatc caattcgtgg agactgataa tttaacagat
    15001 actcccccac agagcctccc gggcaaactg acagcctttt tataccgacg tttcaaatta
    15061 aacaaagctg gctttaccat cattccttgc gaactgattg acagaaacgg cgaacgtcta
    15121 aaagaaatca ttttacgcta cgcagaaaaa tgggatttag aacctgcttt tgttgagtgg
    15181 ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc aggttatcct
    15241 cgcgaagatg caggtgtttt caatcaacgg cataactatg aagatcaatt gatggtcaaa
    15301 gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag aattggagaa tcaactacca
    15361 ttcaaacagg ctggcttgaa tgtcatcatc acagatgata tgactcctta tcgtgaacgt
    15421 aaggtgcatt tgcttaatgg accgcataca gcgatggtcc ctcttgcttt gttggcggaa
    15481 ttggaaactg tagaagaagt aatgaaagat cctttattct ccgcatatgt tgatcagtta
    15541 ttcaatctag aattgattcc tatgctttct ttacctaaag atgaattagc catttatgct
    15601 gaccaaatca aagaacgttt cttgaatcct tttgcccatc acaaattaga agcgatttca
    15661 ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa gtatatcgaa
    15721 gaacaaaatc aggtaccacc gttaatcact gtttcactag ctgcgctact tcttatgtat
    15781 cgaggcgatc aagtaaagcc acatgatgat gaaaagacga taagcgaatt tactgatgct
    15841 tggtctgata atggaacagc tattcctcga ttattgcaaa acgctgcgct ttggggagaa
    15901 gatttgtctc aaataccgaa tgtgacagat acagttcaag aaatagctgc aaagatcgat
    15961 caaaaaggtg tccggtcagt attacaagaa atgaaaggaa cgatggtcca tgcttgaaac
    16021 cctgcaacca acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat taacccctat
    16081 tccaaaacaa acacaactga cgattgatca gcaaacgatc accactcttg aagatatccc
    16141 gcaaggtcat aagattgcat tgggtgattt gaaagctgga gacaacgtga tcaaatatgg
    16201 ctatccaatc ggtcatgtga cgacagacgt aactgccggt caatggctgc acactcataa
    16261 tgtaaaaacc aatctttctg gtgaattgga ttatcgatat gaaaaagatg tccatccaag
    16321 tcattacctt tttgagaacc gaacatttca aggttacttg cgaaaaaatg gaaaagtagg
    16381 gattcgtaat gatttgtttt tagtacctac cgttgggtgt gtgaatggca ttgctgaact
    16441 gatcgtcaaa cagttcaagg aaaaacatcc agatctggga cagtttgacc atattacgat
    16501 tttaaagtat ccttatggct gttcgcagct tggaaaagat catcaaaata ctcgagaaat
    16561 tttagcggac gcagtaaatc atcccaatgc cggaggagtt ttagtatttg gattaggttg
    16621 tgagaacaat acagttcctg aattcaaaaa aattttaggt gaatatgatg aagaacgagt
    16681 gaaatttcta gtggctcaag atgtttacga tgagatcgaa caaggtgtgg ctctattaga
    16741 agagctttta acagcggcgg aaaatgacca gcgcatatca gtcccgctct ctaagttgaa
    16801 tgtcggtttg aaatgcggcg gttctgatgg tctatcggga ataactgcta atcctttact
    16861 tggtgcattt tctgactact tgattgctca aggaggcagc acgatcttaa cagaagtgcc
    16921 agaaatgttt ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag aagtttttga
    16981 ctcaatcgtt gacttgatca atgacttcaa acagtatttc ttgtcttacg gggaaccagt
    17041 ttacgaaaac ccatctcctg gtaataaagc aggtgggatc actacattag aagataagtc
    17101 tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac aatatggcga
    17161 aaaaatcaga aaaccagggc ttagtttatt gcaagctccc gggaatgatc ttgtagctgc
    17221 ctctgctctt gcttcatccg attgccagtt ggtcttgttt accacaggaa gagggactcc
    17281 gtttggctca tatgtcccaa cggtcaaggt atctacgaat acaactcttt ttgaacgcaa
    17341 aggtcattgg atggatttta atgcaggcgt cttattagaa caaccaatgg aagtaatctt
    17401 agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga aaaatgaaca
    17461 aaatgacgtt cgagaaattg ccatttttaa aaacggagta acactttgat tttcataaaa
    17521 cagtataaaa agaaagaagg aagatttatg tttttatctg atgatttttt attaaaagac
    17581 gattgggcga aaaagcttta ccattcctac gcaaaaaaca tgcccattat tgattatcac
    17641 tgtcacttag atccaaaaga aatttacgaa aacaacaatt tcagcaatct gacagaagct
    17701 tggttaggag gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt gcctgaagaa
    17761 aaaatcacag gaaatgcttc tgattttgaa aaatttttag cttggtgtca aaccgtacca
    17821 aaaattatcg gaaatccgct ttacagttgg tcacatcttg aattgaagcg tttcttcggt
    17881 attgacttgt tgataaatga agaaaatgca gagaaaattt ggacacaagc aaatgaaatt
    17941 ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt agaagtgatt
    18001 tgtacaacag atgatcccgc agacgacttg cactatcatc agttgcttgc tcatgaagaa
    18061 ccagatttga aggttctacc ttcattccgt ccagataaag cactcaatat tgaaaaagaa
    18121 ggatttaatg catgggtcac actactggaa gaagcagctg accaaacgat caccacctat
    18181 caagaactga tagaagtctt agggcagcgg atagaatact tccatcaaca tggatgtcgt
    18241 gtttccgacc acgctttaga catcttacgc tataaagaag cggatgaaac agtattagaa
    18301 gagatttttc aaaaagcacg agctaacaaa ctattatctt cagatgaaat cgatgcttat
    18361 cgtacagaaa cactgataca tttgacccac ttctatcatt ctcataattg gacgatgcag
    18421 ctgcacatcc atgcttatcg taactgtaat acacaaatgt tggaaaaatt agggcccgat
    18481 accggctatg atggaatgaa tgatctttct ttgactattc ctctgcaaaa attattgaac
    18541 cgtgcagaag aaacggatca gttgccaaag acgatccttt attcattgaa tccgaatgac
    18601 tatcctgcga ttttagcttt gatgggctgt ttccaaaaag aacagaatgg aaaactgcag
    18661 ttaggttctg gatggtggta caacgatact cgagctggta tgcgcgatca attgacacaa
    18721 tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc tgacagattc aagaagtttc
    18781 ctgtcctaca ctagacatga atatttccgt cgtgttcttt gtgaattttt aggcgaaatg
    18841 gtcatgcgag gagaagcacc tgaagatgaa caacttttag gaagtctagt tcaagcgatt
    18901 agttatacaa atgctaaaaa ttactttggc ttttttgcct aattgattaa tagtaacagt
    18961 aacgaatcaa aaacaggata gtgaaataac ggaatccgtt tatccacgtt ttagagaaga
    19021 agacggacca aatttgctcc gtcttcttct cttgctgttc tgcttcattg cctataaacc
    19081 tttatgaaag aagagataac atgcctacta tccatattgc cggtgattct actgcggcag
    19141 aaaaagatga aaacaagcgt cccgagacag gttggggaga aaaaatctct gcttattttt
    19201 cacccgagat ccagatcaat aaccaagcaa agaatggacg cagcaccaaa tcatttatcc
    19261 aggaaggaag gttagcccgt ctagcttcac agtttcagcc gaatgatttc ttatttgtac
    19321 aatttggaca taacgaccag aaaatccaag aagaaagagg tacgcttccc ttcggtagct
    19381 atttaaaaaa tctgcagata ttttttgaat gcgcaaaaaa agcgaacgtc caaatggtct
    19441 tactgacatc tgtgacgcga cgagattact tagaaaacgg aacattgaat ccagatatac
    19501 ttggagacta tccaaaagcg atgcgtgcat ttgctgagaa acaccatatt cctcttttag
    19561 atgtgttctc aagatcacaa gaattgttcc agaccttctc aaaagaagag acaaaaaaat
    19621 tctacctgca tcttatacct gatacttgta aaaattaccc agaaggacta aaagacaata
    19681 cccactttag tccagaaggc gctgataaag ttgcccgaat aattgttgaa ttgataaaag
    19741 aatcccgtct gcctcttgct aattatttac aaaaaggagt ttgagatgtt tccattaaat
    19801 ttaggcatcc gtgcccacga tttagatgtt cgaaatcgtg aagaaatcgt tcaaaaagta
    19861 tcacagcttc acttgtccca tatccaattt gcaccacaaa aagcatttcc tgaacaactt
    19921 aaattaactg acatgacact tgggagtgct gcctattttg gtgaatactt ccaaaaaaac
    19981 aatatcgaac tttccatttt aggatgctat atcaacctat ccagcaaaaa tcaagaagtt
    20041 cgtgcacaag cattggcaac tttcaaacag caaattgctc tgtgctctgc ctatcaagcc
    20101 aagatgattg ctacggaaac cggtagtgtc tctgtcggtt acacaaaaga aaattttact
    20161 gaagaagctt atcagatcgc cagaaattct atcattgaat tagtcgctca tgcagaaaat
    20221 tttggcgtaa ctgttgctgt tgaagcaggg atcaatcatc caatctataa ctatcagtta
    20281 gctaaacgtt taatcgatga agtagcttca ccaaatttaa aaatcatttt agattgtgcg
    20341 aatttgatgc ataaagaaaa tcatgctgat caggaaaaag tgatccgagg tgccttggac
    20401 aatttacatg gttatattac agcgcttcat ctcaaagact atatcatgga aaatggaaag
    20461 atccgcatgg tacctgttgg caagggctgt ttagatttcc gcccggtact gcattatatc
    20521 aaatatgagc gtcctctaat gtacgccact cttgaagcta ctcctgagat ttatgttcct
    20581 gaagcaatag aacacttgca gtttatttac caagattgtt aaggagaaga ttatggagga
    20641 acgaatacag aaaagttatc aaaaatatca tgcaaatgct tttcagacat tgctgcatct
    20701 ttatcaggga aattattggc gttttgccat gtcaggactc ttttttatta tcaaacatct
    20761 tccatcttgg ttaatgccga tcgctattgc caatgtaatc aatgcagctt catcgaaaga
    20821 ttcttctcag ttgcaggcca tttatttcaa cgcttttttg atggcttgtc tgattggtca
    20881 aaatatattg acgaattatt ttcatgtaaa ataccatagc acttcgatcc gtcaagtaga
    20941 agctggacta agagcgaccc ttatcaagaa aatccaagaa ctatctattc cttatcaaaa
    21001 agaattgcaa tctggacgaa tccagtcaaa gatcatccgt gatgtagaag ctatccaaac
    21061 gttgtcctct caagtatttg tcagcggatt aaatatcgct gtcaatttat tcgtagcttt
    21121 aggtatcacg ctttataaaa gtcctgttgt tttcatgttt ttcttattga ctgttcctct
    21181 cgccgcattc gttactctct ttttccgtaa aaggatcaga cagtccaact cagacttcag
    21241 gcaagaaatg gaagagactt ctgcaaaagt gatcgaagtg atcgaattag caccgattgc
    21301 tcgagctcat gggttagaaa aacacgaaac aactcgctta aataagcatt ttactcaaat
    21361 ctatgaaaaa ggattccact tagatatcct gcaatctgtt ttcggatcag tcagctgggc
    21421 tagttttcag atatttcagc tgatttgttt gattttcact gggatattag ctttacaagg
    21481 gcgcatccaa cccggcgatg tcgtgatgta tcagacgtat ttcacgacga tcgtcaattc
    21541 cgtttctgga atgatcacgc tgattcctac gatttcaaaa ggcatggaat ctatcacttc
    21601 tattggcgaa ctgttgattg ctggcgatgt agaaacttat cacggtaaga aaaaaatcca
    21661 acatttggat ggaaattttc gttttgagga tgtttctttt ctttatccgg actcactcaa
    21721 accgattatc gatcattttt ctctagaggt gaaagctgga gaaacaattg cttttgttgg
    21781 gccatctggc tctggaaaat ctacactcct taacttattg attggattta tccagccgac
    21841 ttcaggaaaa atttttctag atgatcagcc tttggatgaa ctggatatgc gaagtgtgcg
    21901 aaattattta gccgttgttc ctcaaaccac tttgttgttc tccgcttcga tcaaagagaa
    21961 catcacttat ggcttgaaaa acgtatcaaa agaacgttta gatgaagtca tcgaagctgc
    22021 gcaactttct tcactgattg accagcttcc tgatggtctg gataccttag taggcgaaca
    22081 tggcaacaag ctgtctggtg gccaaaagca acgtatctcc attgcacgag cgttgatacg
    22141 tcagccaaaa atcattttgc tagatgaagc aacctcagct ttagataacc agtcagagaa
    22201 aaaaatccag caagccctga attacctgac agaaacaccc actactttta ttgtggctca
    22261 tcggctttca acgatcaaag aggcagataa aatcgttgtg atcgatgaag gaaaaatcgt
    22321 tgaaataggt acttacgaaa aactgttagc tcaaaaaggc tatttctacc gcttgaaaaa
    22381 tgaatgacaa aaaataggag ctatgacaag acttttgtcg tagctcctat ttctgaataa
    22441 acggtgttct cctgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc
    22501 tgttttttga aattgttcct tgtaactcag agttaaacgg taggctcacg acctcttggt
    22561 aaatggtgtt caagagacag cctatcggca ataagaagca attttgtcat cgctcaaggc
    22621 cgaacgtctc tttcacaccc tctgttgttt cataaaatag ttttattcgc tgtaaaggtc
    22681 gcgaactggt gtcatgatga tacggttcaa gtcattgatg attgtactga agccttgctc
    22741 tttttgcatc aagtcattga ttacttcttc tgattggatt ttcacagcca tttcttgtgc
    22801 tttcattgca tcttcgtcgc tgaactcttg tccactcatt tgtttttctt gtaattcgat
    22861 ttgcatcgct tggaagtctt tgaacagttt gtgtgcttct tcgtttgctt gtactttctc
    22921 gtacgcttct ttcaacgctt tgaattctgg taattcacgg atctcacgtt cgatttgatt
    22981 cgcgctatcg tagatgtttg acatgttatg ccctcttttc ttttagtagt tactctctat
    23041 tgtaacatgg ggttattcgt tttcaaaggg gctactctcc ttgatatttc cgccagaggt
    23101 ctttagccgc ttcaccaatt ccctgaacgc cttctttcac catatcgccg aagtttttca
    23161 gcccttcttt tgcttgttca ccatattgat tcaagttatc ttgccaattt ccagactctc
    23221 cattagaatt tcctgtgttt ccaacttgat ctgcagctac cacttgtcct cctgtggcat
    23281 aggcatctgc tacactaaat cctgtttttg aagtatacgg aaggatgcct tctgccgctg
    23341 atttgaagac tttcccgaca acatttccac ttgtcccttc caaatagtgc agcttgctgg
    23401 attcttcgta acccatccat gtcgaaatca ctacgtctgg cgtgtaaccg atcatccatt
    23461 ggtcatttgc ttttgtggca tcgaaattag tttctgtggt tcctgttttc cctgctatcg
    23521 tataacctgc aggattcgca gcagccgctg ttccatttga aaatgtacca agaagcatgc
    23581 tggtcatttc atcggctgtt tcctttgata tgacctgttt tggttttttg tttgtgttat
    23641 cgacgatcac cgctccagtc gaatcaacga ttttcgtgat aagatgcggc gtatacatct
    23701 gaccatcatt ggcaaatgca ctgtaagcac ccgccatgat caatggagat actccttttt
    23761 ccagtccacc aagggctaag ccataatact tatcagattt tgctaaaggc aggccgaatt
    23821 cttctgcttt atcatatcct ttttgtaaac cgatctcatg caaaagccat acagtaggca
    23881 aattcaaact ttgtgcaaca gcttgataca tcggtacttc tccttggtat gtcccgtcaa
    23941 aattatgcgc atcgtaataa ctttgcggtt cgtctttcaa gatactggaa ggcttgtatc
    24001 cagcttccag tgctggtgta tatacgctta atggtttgat cgttgatcct ggtgaacgac
    24061 gcatctgtgt agcaaagctg aatcctctaa agacatgatc tcctcttcgt cctactagtg
    24121 cttgtacacc acctgtattc ggatctaaag ctactgaagc agattggacc atggcaccat
    24181 ctgaagcatt tgctgggaat aatgcactgt tttgataagt agcttctaat tgcttttgat
    24241 aatcctgatc taaagctgta tagattttat agcctttatt catgacatct tcttcatcaa
    24301 gaccatactc gttaactgct tcatcgatca ctgcatcaaa ataataagga taacgataac
    24361 cagaatctga attatcgtat gtgtcattaa gatacgcgct gatatctgtc tggctttctg
    24421 cttgttcttg ttcttttgtg atttttccgt tttcttccat tacacttagt actgtgtttt
    24481 tccgatcatt cgcatttttt gggtgatcga tcggattata aataccagga cctttcagca
    24541 ttcctgccaa agtagcagcc tcagacagcg taacattcgt tgcatctact ccaaaatact
    24601 ttctggaagc gtcttggaca ccccataccc cgttgccaaa ataggcattg ttaagataca
    24661 tggtcaagat ttcttctttg ctgtacttct tttcgatctc gattgccaaa aataattcct
    24721 tcgcttttcg atcaaacgtt tggtctaatg tcagatacgc atttttggca agctgctgag
    24781 tgatcgtact tcctccgccc cctgatgttc cgccagacaa gaccattcgg actgctgcac
    24841 gggcaatccc tttgatatca aacccgtgat gctcgtaaaa attcctatct tctgtcgaga
    24901 ttactgcatc ttggatatag ggagaaatcg cgtttaattc tacatatgtt cctttttggg
    24961 cgtacagtgt gccagcttct tcgtttttct tatcataaat gactgtcgat tggcttaatc
    25021 ctgattttaa cgtttcgaca tttgcttgtt ttgctaaaat aaagaggtag atacttgtca
    25081 ctaagacaca gatcaaacca atcagcaaaa tcaatttgtt cacatgatat tttttccaga
    25141 tgcgctttcg ccatcgatgg aattgtccga gatatggtct gatccaacgc caaaaactga
    25201 tcaaagcagc ttttatcttt tctaaaatgc tttgaaaatc catttgcttc ttctcctgtc
    25261 tatttgattc aatcgtctta ttttaccata gtgaaagagt gttttcttcc tgcttaagtt
    25321 gtagttcaat atgttttttt acgttagtgc aagtagagta acataagaat gaacagaaaa
    25381 atgtaagttt tttcttaaat atgttttaaa aatggtacaa ttagtctgcg attgcactga
    25441 aaggagtaca cttgtgaaaa tcacgatcac tttacctatg aaccatcctg aaacaacaat
    25501 tcgcgacttg cttgaaaagg aatggttagt tcctagaaag gttcgacact ttttgcgtat
    25561 acgtaaaaac gtttggatca atcaacaacc tgctttgttc catcatgaag taaagggcaa
    25621 tgaccagatc acattgttat tcgaagaaac agattataat tatcaagaag ttcaattagg
    25681 agatgcgaag cacatccgtg ttttatatga agatgagcat ttgattatag tggataagcc
    25741 tgcaggaatg aagactcatc caaatgaacc gacagaaaac agtaccttac tcaatcattt
    25801 agctgcttat ctcgacacta aacagcaacg accttacgtt gtccaccgct tggacaaaga
    25861 aacaagcggt gccgttttat ttgcaaaaaa tccatttgtc ttgccgatac ttggaagaat
    25921 gttggaacaa aaaatgatct atcggagata tcaagccatc gtatggggag aaattgccaa
    25981 agatatgacc atcaccaata aaattggacg agatcgccat gatcggagaa aaagggtcgt
    26041 agatgataaa aatggtcaga gcgccttgac acatgtcact gtcaacaaag tactttctga
    26101 acaaacacaa gtctattgtg ttttggaaac cggacgtaca caccagatca gagttcattt
    26161 agctcataag ggacatccga tcgttggaga ccctctctac caaaatagaa cagctgaacg
    26221 actgatgctc catgcattag aactgcacat gatccatcca tttactcaag aaaaaatagt
    26281 cggtaaagct ttacctggat tatgggaata gaaataaaaa agaacagagt cttatcagca
    26341 gataatgata agactctgtt ctttttgtgt aatttgtctg cttacttttg atgttcgtgt
    26401 tctgctaaca gcaaactacc gataatccca ctttcattcg gcagtcccca acgaacgata
    26461 tattcactta cttgaggtgt ttccatataa ccacccatca attcaacaaa ttgctgacgg
    26521 attttttgaa ggagatgatc ctgattcatt accccaccgc ctaaaataat cttttcaggt
    26581 gctaacgtca acgtatagtt gatcagtgct tgagcaatat aataagcctg aatagcccaa
    26641 acaggatgat cttgcggcag atcttgccct ttgatccctg tgcgtgcctc aatagacggt
    26701 cctgctgcta atccttctaa acaatcttta tgataaggac aagtaccttc ataatggtca
    26761 tctggatggc gtttcaccca aatatgtccc atttcaggat gtgcgatccc tgaaaaaatt
    26821 tcttcattga tcacgacacc tgcaccgatt cctgttccca cggtaaggta aatacaacta
    26881 ttttttcctt gtgctgctcc ttgcttcaat tccccatatg cagcagcatt gacatccgtt
    26941 gtccaagcag caggaatatt gtagcgttcc ttgattgtgc caagaaaatc gaattgtgcc
    27001 cacccttttt tgggagttgc caaaacgtat ccgtaattat cttttgtttt gtcgatacca
    27061 atcgggccga atgagccaat cccgattccc ttcaattcat aacgatcaaa aaattcaaat
    27121 acctgatcca atgtttcttc tggtgttgtg gtagggatac ttaatttttc taaaatttct
    27181 ccaccatctg aaaccgcaca cacaaatttt gttccgcctg cttctattcc gccatacatt
    27241 cgttttcctc ctcgaatccg attgtccgaa tctcataagg ttggattatt gggacaaccg
    27301 cttgcttcgt tggttcttca agcagattca atatgacagt ttttccattg tctttagtta
    27361 tttccaattt ttctaaatga ctagacatat tgaaacctct tactactact ttatcactaa
    27421 acttgctgcg tttcaaagct gtaattgcaa acgtttcact ttttatcgtt aaatattgtt
    27481 gttttgaaat caatgttcct tcatgatgtt tgatttgttg agtggaaaaa ggaatctgtg
    27541 ctgcgtatgc atgcaaataa gtagagaatt tttcttctgg tccatgtagt tcaatgctgt
    27601 aattgaaccg atgctcacct agacactgtg cttctggtgt tggaaaatat ccccaatcgc
    27661 ctagttctcc cacactgcga agtagcgtca ctgcgatttg tccgtcttct gttacttcat
    27721 attcattcaa accaaagttt ccgactgtta caccgtactc ttcatcatga atattgacaa
    27781 atgcttgctg atgttgcgga ttcgtggggt tttcccaact tttagacaca tggtttgggc
    27841 gcttcaccac ttcaaaaata ctatctgctt cgtgatgttc cacatgtaac tttgtaggaa
    27901 acaagacacg taagcgatga tctttcatct gattgtcgag agtcgtttca aagtcgattt
    27961 ttttactatc ttttctgatt gtcatgattg tttttatttg aaggattcgt ttttctttgg
    28021 atcgttctgc ttttcgataa cgaaattcca caaccatctg ctgttctttt tctaaacgct
    28081 cgtctgcaga aacagggatt tccatttcct gtagaatact tatttttgct atttccggtg
    28141 tatctactaa tacttttact tcagagtttt ctttgttact ggataggatt ggctgatcac
    28201 agaaaggttg tttaaagata tactcatttc ctatatcgcc gatatcttca aaaatatgca
    28261 gattattcaa ctttcgtttg tttttgcgat cttccattgt aatggtacca tttttttcaa
    28321 tcgtcaaatg tagagggccg ttttcgatga ttcgtccact ctgatgaatc attgtttcct
    28381 tttcttctgc tttcgcttcc ccttctacaa gcgcgaatgt ttcccaagaa aatgctggca
    28441 tgttttctaa tggtaaagtc actttgatca ttcttgccat atatggcaca cggaaacggt
    28501 ctttcggcaa atcataacca aattggacag tttcttcaga taaaatagct ggtatagtgg
    28561 ccccactttt tgtttctaca tgatacttac gtttaggcaa attttctaac tcttgatata
    28621 gttgttcagg tattccttca gcgaatcttt ttctttctaa ttcgatggtt atttctgctt
    28681 caccagtttt atccattcct gcagtattta caatgacaaa tggaaagctg tcttttggga
    28741 atccggtcgt atcgattgct gcggtcagct gttctaagga atcttgtgca agatactttc
    28801 caacttcatc tgctttttca aaacgaggta tcatttcccg atggacagaa tcgactgaac
    28861 aaccgcaaat actgtcgtga ggatgatttt gcattaatgt cttccaagca taatctaatt
    28921 gatcatgggg atagtttcca ctaacttcat aagccatcgt cgcaagtggt tctgtaatat
    28981 tttctagctg tcgctggacc ttggtattcc attgttttaa gtaaacccga gcagatgctg
    29041 tattggctaa agtataccag ccatccgttt cttgacttgt caattctcct tctactgagc
    29101 ctaagtcttc tggcacatct ttttgaactg cttccagata gtcagtaaaa ttagaatgaa
    29161 taaactcgta atctggaaac agttcatttg cgagatggat tgcttttgat atatcttttt
    29221 gtaccggttg atgatcaaca ccattcatca tcaataagtg gttcgtagag gcatattgtt
    29281 ctgcatccgc taatttttgc ttccaaaagg caagagctgc ttctttttct actggaatct
    29341 cattcccgtt gctataccaa ttggcaaata gcagaccaaa gattgctgtt tgatcaggtc
    29401 ctttccacca catctctgag tattgggaag aatagttttc tgcttccaaa acttgattat
    29461 caaagccaat tggttttaca ccacggccaa acgctgctgc tgaaatacca gcttgtttca
    29521 ttaattgagg cgtctgtccc atatttccaa atgtatcggg aaaatagcct aacatgacag
    29581 gggttcccca tttacggctt tcttccatgc caatcagcat atttctcacg ttcgattccg
    29641 agctgataag aaaatcatct tgcaagatat aaaatggtcc aattctcaat tttccttcat
    29701 tgattgcttg ttggattgcc tgtctttttt ctggccttac ttgaagatag tcatctaaaa
    29761 taatcgtttg cccatccaaa tgaaaactgt tgaaggacgg atcaacttca aatagctcta
    29821 gtagatcgtc tatcaattca atcagtcgca tgtgatgctg ttcgtaagcc atgtaccatt
    29881 cacgatccca gtggctatga gaaatgatat atactttctt tttcatttac gatacctcct
    29941 acttttctac tctaatatca aagtaatcca tgactaattc gcaaaacatc atatttgccc
    30001 aagaaaacca ttctctcgta taattttcag gattgttcac atcgaatcct tcgtgcataa
    30061 ggtgtgttcc tgcgtcagtt gctacaagta aattcaatat ccgctctttt tcagatttgt
    30121 ctgacgttgt cattccttcc atagctaatg caatcggcca aacgtatttt tctggcgtat
    30181 gggaggatcc aattcctttt gcgtactttc cttcatagaa atatggattt tctttactca
    30241 acaatgttct tcttgtttgc agataatctt cctcttgttc agaacaatat cccaagtaag
    30301 gtgcggcgat cagattgggc acattactgt catccatgac tgtggcgttt cccattccat
    30361 ctacttcata tgcataaatc gtttgccctt tttgattaac cgttcgtccg tgattctgaa
    30421 tcccctcttg gatctctttc ttcaatttct gtgtttgctg tacaatttct ttatcatcga
    30481 gtatcgtttg aaaaatttct tccacataac ctaacacgac caccgcaaac atattggatg
    30541 gtaccagata tccataacgg caagtatcat cgctaggacg gaaaccagac catgtcattc
    30601 ctgtaaaagc tgtttttgca cctcgcccgt cattgatcag cgtatcttct tttcttgtcg
    30661 tgtctcgtac aaatgtataa ggagagtgtt catgatcttg ctctgttttg aacacgtcca
    30721 agattttttt gataccttca ataaaagatt gattgaattg atcagttttt cctgtatttt
    30781 tgtaaagaag ataggccaac tgtattgggt agcataaaga atcaatttca tattttcttt
    30841 cccagatcca atcgttcatt tttgtatgat ctgtttggtg gccagcaccg ttagcttctt
    30901 cgttgaaggc attcgcatat ggatcgatat tgatataaaa aaattgcttt ttgactaacc
    30961 cgctgatcat gtcagctaat tcctcatctt cttttgcaat cactaaataa ggacgaactt
    31021 gtgcagtaga gtctcttaac cacatcgctg gaatgtctcc cgtcaataaa aaggtggttc
    31081 cgtcattttg ctttttgact gttgtcaaca gagtattggc aaatgctgct gtaaaattct
    31141 ttgcccagtc cgcatggata tccccacatt tttgtgtgat atcttccata aatttctgca
    31201 ccgattttgg tatatcttta taagccacta ataaaacctt ctttctattt gatatatctt
    31261 taaagattat acttcataca gccaattatg tctacgcttt caaaaaatat ctttttattt
    31321 actttttcat ccattatggt attatttgaa tatgatatat cattaagagg tgtttatatg
    31381 aatcaaccat tgtaccagac aatatttaat gatttgcaaa cggctatttt aactggtgaa
    31441 ttacctgtag acagccaact accaactgaa aaagaacttt cacgtaccta taaagtaagc
    31501 agaatcactt ccaaacgtgc attgactgaa ttagagcaat taggacttat ttatcgcgtt
    31561 cgcgggaaag gcagtttcgt taaatccgct tctccggaac agattcttgc ttctcctaca
    31621 aaaacacgac ggattctgtt tcttcttcca tatgtcgctg atttgtctgt tggtgatttt
    31681 agcaagggat tgaaccctgt gatgcaagat aaacagattg atgtcatgat gacgacgctt
    31741 gactttctac aaaataaaac agcaaaagag ctaatcaatg aatttgacgg attgatttat
    31801 tatgctcttg atacagaaca acatcttgac ttactttttg agctttcatt gaaagaattc
    31861 cctgtcgtca tattagataa aaaaatctat gagttacctt ttcctaccgt tctttccgat
    31921 aatttccaag gcggtgcgct tgctgcctct tatttgatcg aacagggaca ttcaaaaatc
    31981 gcttatttgt ttggtacgca gactcatcca caatccgttc gacagcgtta ccttggttac
    32041 cttgaagcat tggacaaagc tggtttgact ttccacactt cactggatga caaaagcgcg
    32101 acattagatg aattgctttc ttatatcact aagtatgaag taactgcttt tgtttgtgaa
    32161 aacgatgttg tcgctatcca gaccatgaat ctcttgagac agcatggtta tcatatccct
    32221 gaagattttt caattatcgg atttgatgat atccaagcag cagctcttgt tgacccgcca
    32281 ctgacaacca tcgcacaaga ttttgaacag ttaggtaagc ttgcgggaga tgctttggtc
    32341 acttggttag agacaaaaga tcgaccatcc gatagtaaat ttccagtttc attcatcaaa
    32401 agacaatcaa caaaggagaa ataaaatgaa tatcggtcaa atagatacta gacatgcaac
    32461 agcgaatcag ccaaatttct ctaatgggaa ctgccagcct tatactggcg ttccttttgg
    32521 gatgaattat tttgcaccgc aaactacgga tcaaaacggc agctggtggt ttcatcctga
    32581 tgaccgcgtt tttcaaggct accgtcttac ccatcaacca agtccttgga tgggtgattt
    32641 cagtcatatg ctgtttacgc cgatcaatgg aaaactacag gagaacaccc ttttccatgc
    32701 tcaaagttct taccgcccag aagaaagtat attttgcccg actcatctgt cgatctgtca
    32761 attacgttat ggcatctgtt ctacattgat acctagcatg tatggcggaa tcttatctat
    32821 tgattacagc aaaaatgaca gcggtttact tttatccttt ccgggacgcc atcagctctt
    32881 tgttatagat ccttacacag tggaaggaaa aatcacacat tttgctggtt cagaagatcc
    32941 tgatttttct ttttatttta tattgagatt tgaatatcct ttaacaattg atgagttagc
    33001 tgtttccaac aaaaatagcg actcgatccc gttgtatttt gaacagacaa aaaaacaaac
    33061 tatccgattt gggacatctt ttatcagctc ggaacaagca gcttataacc tggaactaga
    33121 acaaaaccaa agcaaagaga cttaccttgc tcacagtcaa gaacaatggc agtcctattt
    33181 tgatcgtata cagctcacac accatgatca acaacaattg cggacgtttt atcataattt
    33241 atatcgggtg tttttatttc cacaaacatt ttatgaaatt gatcctagtg ggcaaaaaat
    33301 ccactatgat acgactagtc gatctataaa acctggtgtt ctctatacaa ataatgggtt
    33361 ttgggatacg tataaaacgg tttatcctct ttattctttg attgctgtag aaaaatacga
    33421 agaaatgctg gaaggttttt tgaacagcta tagagaaagt ggctatttgc caaaatggct
    33481 gtctcctgat gaacgtggac ttatgcctgg gacattgatc gatgcggtca ttgcagatgc
    33541 tgctgtaaaa gatatccgac gagacttgat gcctgaattt ctaacggcaa tggaaaaagg
    33601 tgctacggtt caaagcaaac atccaaatta tggacgccaa ggaacagaag attacttgac
    33661 atatggctat gttcctaatc actaccacga atcagtcaat cacacattag actatgctta
    33721 tagcgatttt tgtattagcc aagttgccaa ggtactagaa gaagaggaaa tgacgaagaa
    33781 atatcagcag caatcattga actatcaaaa tatctttgac tatgagtcag gatttatgcg
    33841 ttcaaaagat aaagatggca agttcagaga accattttca gctaatcgtt ggggacaaga
    33901 ttatgcagaa ggaagtgctt ggcaaagcag ctttgctgtt tatcacgatt tttcaggact
    33961 gatcggaaaa tatggtggca gtgagagata taaagaaaaa ctcattgagc tgtgcaatca
    34021 gcccccaacg tttgacccag gaggatatgg ttttgagatc cacgaaatga gtgagatggc
    34081 cgcgatagaa tttggacaat tagctatttc aaaccagcca agttttcact atccttactt
    34141 gttcagttat ataggacaac ctgaaatggc acagccactg cttaaacagt tgatgactca
    34201 gaccttcgac gattctccaa tgggttaccc tggagatgag gacaatggaa gtatggccgc
    34261 ttggtatatc ttcaatagtc tgggatttta tcctgtcacg cctggaaccg gtgaatatgt
    34321 tatcggtatg ccattaattg acaaggctgt tttgcttttg tcaaatggaa aggaattgac
    34381 gattgaagca acgcctaatc ggccgcagca acaatttatc cataaaatca aacggatgga
    34441 caaagaatat acgaaattat attttaccca tcaagatttg ctgaaaggcg gcacgatctc
    34501 ttaccaatta gggatcatcc ctcaccctaa gaaatatcaa gcaaaagatt tgcctttttc
    34561 cttatcaaac tataattaac aagaggttgt gaaaaagctg tcctgcatca agtaataaga
    34621 acagccaagc aaaaatagat cctcatattt ttcgtttggt tggtcttatt accgcagatg
    34681 caagcttttg aacaccgttt attgagaggt tgtgaaatca gcgttctgac ctgagtatta
    34741 gagagacaaa tggcaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg
    34801 tcagctgatt gaacaccgtt tagtggagag gtcgtgaaaa agctgtttag tttcgagtat
    34861 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga
    34921 gaaactggct tttgaactcc gtttatcgag aggtcatgaa aaagctgcct tgcctcaagt
    34981 aataagaaca gcccaaaatt agatttctag acaatttttt ataagttatt tcattacatt
    35041 ggctttttct tttatgattc tccactgttt tctcggtact ctttagggga tctgccgacg
    35101 attttcttga ataatttaga gaaatacgtg gtgttgttgt agccaatctc acttgctatc
    35161 tcattgatgt tttgattact gtgcaaaagc agttgtttcg ctttttccat acgtaaatgg
    35221 ttcaaataag ctgaaaaact ttttttcgtt tcttttttga acagctgtcc tagatacatg
    35281 acatttaaat gcaggttttc tgacacttct ttgagtgtaa gtggttcttg ataatgctgg
    35341 tgcaggatta cgattacttt ttccacattg tcactgtact ttttctgtct ctgatgcttt
    35401 ttgatgaatg acagaagtag ctgctgaagt tcttgcaccg tttttgcctg atggattttt
    35461 tcgattcctt gtaaatattc ctcatcttcc agatcaatca attcccgttg aatctccata
    35521 aacagtaaaa aactggaata tcgtatatct tccggcatca tggctgctaa ctgaaactgt
    35581 tcaaaaaaat cagtgatcat tttttgagcg acttccaatt gtttattttg cagcacgcgt
    35641 ctgaaacttg aaaaatcgat ggtttgatct tttgtttctg cttgtactgc gtaaaaaaca
    35701 tgctcgtctt tatctccata aaattgatgc aactgtagat tgtctttcgc ttgttggaat
    35761 gtttcaggga ttttgtccaa agaatctgtc tcttccccga tactgatcag ccattcctga
    35821 tcaggaaagt tttcttgaac aaaacaacag aagccatcaa cttcatctgc ctccaacaac
    35881 aacgtgatca atacacggtc tccgtaattt cgttgatagt aaaagatttg ttgccgttct
    35941 tgcaaccatc tattgataag agtatcatga gttcttgcca gttgaatcaa tactatccta
    36001 tgctgcttct cacttatttg tcctattatt tcttcttcac ttatttcatc caattcttca
    36061 ttcaaccatt gactgaataa cagttcttga tagatttctt gttgattttt ttgttcattt
    36121 tgctgatcta accgtgtttt gattttcttg aggctttcaa ccaattcgaa cttattcacg
    36181 ggtttcatca aataattcac ggcgcctaat tgcatacctc ctttcaaata atcgaattct
    36241 tggtatcctg ataaaatcat aaattcaaaa ttatgacgtt ctttttgagc cgcttcaata
    36301 aattccagtc catttatttc tggcattgtc acatccgtta ctactaaatc aatctgtcgc
    36361 tgttcaagta cagctagtcc ttctttggca ttctctgctg ttgctactat ttgaaagcct
    36421 aaactttgcc aatcaattat cttttttaac ccattcaaaa tcatgtattc atcatctacc
    36481 aataacactt gatacacgct catccctcct tgaatgtaat agaaacggtc agtccacctg
    36541 tctcattatt ctcaagcgac atataataac tgctgccgaa gctggcacgt aaacgttcat
    36601 tcacgttttg cagaccgata gaaccgtgaa gttccacttt aggatgagac agtttttctt
    36661 caatctgttt tagccgtttt tcagtcatcc cttttccatt atctttgatg atgatcctta
    36721 cccgtttgcc ttcctgcaag actttgacac ttagcgcatt atcgaatcga gtaaaatcaa
    36781 taccgtgttt aaaataattt tcaactaatg gctggatcac aaatttaggt acttcgatct
    36841 tttccaaatc aggatcgatc ataaaatgat aggctactcg attaggataa cgcatttgat
    36901 acaaatagac atatttttcg caaaagtcca gttcttcttt taaagtgatc gttttctctt
    36961 gattcgtatt gtttcgaagc aaagcggaaa acgcatacac aacatctgct aattcttcac
    37021 ttccttcgct gattgcatac attcggatat attctaatgt attatataaa aagtgtggat
    37081 tgatctgtgc ttgaagcgcc cgcatatgtg cgtcttgctg tttgatttct aatttgtaga
    37141 tgtcttctac aaactgattg atgctatcca acatttcgtt gataccgatt gaaagttcct
    37201 tcagttcata ttccgtgttt gttatgtcga tccgcgtctc taaattccct gctgctgttt
    37261 gatccatggc ctgcatgacg atagatactt gctgtgaata tcgtttgaat gttttataaa
    37321 gaaagataag cagtatattg tctaaaataa tgataccaat gatcagcggg gcaagatttt
    37381 ttcccgtttc gatttttaca ctttttttat ctagtacagc taatatacga taatcatcag
    37441 acagttcttg gtgttgaaca aaattgtttt tttccagatc gtcaatcggc aattgtgaat
    37501 ttttcttcag actctctgtg atgatcattt cttgtctttc aaacgtttct ttagctattt
    37561 tattatcatg gaatgtgtat agtcgatttg tcgttccaga aatcatgtat aaagagagtc
    37621 cattgaatga cgttagattt tctaaaatca tatctagttc tttcttttga aaaccaataa
    37681 aaatacttcc tgactcagct cctccttgtg tgataggcaa cttgatataa aaggcgtcta
    37741 gtaattttgg tgtccccttt tttatttccc cacctttgtt ttctctagaa gattcataat
    37801 actccggtag ttgattcaac acgatatata tcgcacttag ttcttcataa tttgcgtaga
    37861 gatctttgat ttgatttggg aaagaaacta attcatcggt actgttttgc tgttcgtata
    37921 catacatcag atactgatct atgggttttg ttaagtaggt cgtcacattt tcgatctttt
    37981 cggaattccc tacaagctgg tttttgatga tcctactcaa ggttgtttta tcattcagca
    38041 ttctgcttgt gctttgaacg gcatcacttg tcatgttttc tgcctgtttt tgattttgtt
    38101 cccaagtatg gatactaaaa ataacggtag cagtcatcgt tacaccgatc atgataaccg
    38161 cgtatttccg caacagacga tttagtaagg ttccttttga aaatagaatc cgaaacttat
    38221 tcataacttt ccaactgttt atctaattct tcacgccaat gtgttgtcat cgtaccattc
    38281 caaaccgtca aaagaccaat gacaccaaac aggatcaatc ctttgaattg ccaagtcacg
    38341 cctaaaatta taccgctacc aataattatt tttagaaaag acgaaaagct ggaaaaaacc
    38401 gaaataaaac ttagctttag cagattagga acagagatcg tatattcact atctaaaatc
    38461 atactgtatt gataagtagc aaataggcag agcaaggcaa aaagtaaaat aaaatcaata
    38521 gctaaaaaaa tgagcccttt gatttgaaca gataaatata aattgtaact caataaagca
    38581 gtaccagata aaaataaact aaatagcaga ttgcctctta aaaaatttct tttatatata
    38641 ttccagcctc tttttattgt gatttcttta tactcgaatc cgtacaaata aaatagttca
    38701 ttgactgttt tccatgccgc acctaatcca aagatgatcc cgccggcaaa tgtatatatc
    38761 cagaaataca aggttagttt tataatgacc cagatccgga taaataactc ttccaaaacc
    38821 ttccctaaca tatcttctcc tccaaacatt aagtttatac tttctcatta tacaaaaaaa
    38881 ggttgggcaa tcagcacctt gacctgatta ttagagaaac aaacggaaaa atagttcctc
    38941 atattttgtc gttttgtgag cgaataccga gggttagctg attgaacacc gtttaccaag
    39001 agtttgtgaa atagccgctt agtttcgagt agtaagaaaa attttgtgaa tatggctttc
    39061 cacattttta catatattta gcttaatacc gagaaactgg cttttgaaca ccgtttattc
    39121 ggaacaagag gttgtgacac gtcttttgtc acaacccctt tgtctttatt cattattatt
    39181 cttgtcggaa ctcatcatat tgtttttgca tttctttcaa taccttatca tagccagcct
    39241 tgtccaaagc gtctttcaat ttcggtaatg tttcatctgg atcaacagtc cctgtattca
    39301 atccatctaa atattgattc atgacattag aaatattact taattctgtt ttgacactgt
    39361 cagtgttgaa gctgaatcct aaaatcggtg aagtttctgc atcagcaatc gattgatcac
    39421 gtttggcaat catgtcatcc gtaatagact cttgtgtata tagaatcttg ttgtttcctg
    39481 tattccaagc agacatatgt gtatttggct gataaccatc tagcaatttg attttatcat
    39541 ctgaaccatc gactttttcc catgcttctc cctcgatccc ccaaacaagc ccattcaata
    39601 attctggatc actgttaagc agccctagaa gttctactga tttttcttta ttttttgaag
    39661 tatttgatac gacaaaattt gccatttgtg cttgagctgt agattttagt ggaacagtaa
    39721 ttgcttttga gacaagttct tgttttgctg catttgtcaa aattgtatcg ccataatcat
    39781 acggtccttg tgtttctcca cgcattagcc aagtatttcc ttctagtgga taatccttat
    39841 tgctcgttgc agcgtcactt ggaatgagtc cttgtttgta ccagctatgc attgtacgta
    39901 acagtccgat atacgtttca ttatcgtatt ggttgatgat tttcgaatca tctccatcca
    39961 gatcaatagc aaatggtaac gtattcccta aaggataatc aaagttccct tggactttga
    40021 agccttgtcc aatggcaaaa gctgccgtgt ttggttcttt ttcatggaat tccttcaaga
    40081 ctttttctgc atcagcgtaa gattggattc catcaattga aagatcgtat ttatctagta
    40141 aagctttgtt aaaagtaagt acttgttgag caaagacatt tccattgact gggaaagcat
    40201 ataattttcc attgaccaga tttcctttaa tatatgcttc atctaaatca ttataagctt
    40261 tttcagcgta cttaggcgct aattcagtca aatcagcaaa ggctcctttt tgggcattgg
    40321 aaacatagtt gttcgcaaag gcgatatcat aattttcccc ggaagaaatg atgacgttca
    40381 ttttcttttc gtaatcgccc cagccaatgt aattgatatt gacttttaca ccgatctctt
    40441 tttcgattcg tttgtttgct acttccatca attgatcgaa attatctggt ttatctccga
    40501 tttgatacat ctgcaaagtg tccgaattgc tagcttctgc ctggtcttta gaaccagaac
    40561 ctccgcatgc tgctaatgtt cccaacaaca acgctgaaac acctgtaaag gctagtttct
    40621 tccataactt catttttctt cctcctaatt ttatgattta tatttttgtt tctttattct
    40681 tttactccgc cgattgttag acctttgaca aaatgcttct ggaaaaatgg atagctgatc
    40741 gcaataggca acgtcgagat gactacgatc gccattctgg cagattctcc tggaactgag
    40801 gctaatccgc ctgtcaattg gcttcctgct cctgaatttt gtgtcagata ttgaatatta
    40861 ctttggattt tcatcaaaag atactgtagt ggaactagct tatcatcttg gatgtatagt
    40921 aaagcattga accagtcatt ccagtaaccc aatgcggcaa ataaactgat tgtcgcaata
    40981 cctggaacag ctaacggtaa aacgatctga acaaatattc gcagttcgct agctccatcg
    41041 atccgtgccg attcaataat cgaatcagag accgaacgtt taaagaatgt gcgcatcaca
    41101 ataatattga agggactgac agccattggc aagatcaatg cccaaatcgt atcttttaac
    41161 tgaagaagat ttgtcataac aagataattg gccaccatcc ctggtgagaa caacatcgta
    41221 atcaaacaaa aaacggtaaa aaaacgacgg aatggaaagt ttgaccgtga aatcgcataa
    41281 gcatataaag aagtagccgt gctattgatc agtgtaccta aaacagtaac aagtacagtg
    41341 acgaaaagcg cttggaaaat tttatcctgc atctggtcga acaagtacgt ataaccaaat
    41401 atactgaact tttctggcca aaaactgtac ccgtgagtcg acaatatgga ctcatcggta
    41461 aaagagatgg cgataacaaa tataaatggc aaaatacagg aaatagcaaa gagtgcaatc
    41521 attatattag agaaaaaatt agccgtctta ttgaaagagc ggacttggac tctctccact
    41581 ttcttttttt tcattttttt cctccttaga ataatgcaga atcgctatca aaacgtcggg
    41641 cgatcgtatt tgtgatcatc aacaagacaa agccgacaac tgattggtaa agtcctgcag
    41701 cagccgtcat accaaaatct cctgtggatg tcaggccatt gtagatatac gtatccaaaa
    41761 cttgagtcac atcatataat gcaccggaat tcctaggaat gttataaaac aggccaaaat
    41821 cagctcggaa aatgtttcct acggctaaaa tcgttaaaat cgtcattagt ggaataagtt
    41881 gtggtatcgt cacatgtttg atctgctgcc atttgctagc tccatcaacc atagcagctt
    41941 cataataagt cggatcgatt cccataacag atgcaaagta aataatgctg ttataaccaa
    42001 tccctttcca tgtaccaatg aacaccaaga taaacggcca atatttcggt tcattatacc
    42061 aattgacagc ttctcctcca tgtccagtga tccactgatt gaatattcct ttgtcaggac
    42121 ttaaaaaagc gtaaacaaag taactgataa tgacccaaga tagaaaataa ggtagtaagg
    42181 acatcgtttg ataaacttta acgagtcgtt tatttctcaa ctcgctcata atgattgcaa
    42241 atacgactga gatcagcaga ttcaaaagga taaatgctac attatataaa attgtatttt
    42301 tagtgatgag aaatgcttga tccgaagaaa ataaaaattt gaaattttcc caacccaccc
    42361 aaggactttc cctcaagcta gccaagaacc catctgctga aatatgaaaa tttttgaatg
    42421 ctacgacatt cgccattaca ggtatgtaaa agaaaaaaat catccaaagc atacccggta
    42481 cagccatcag gacaagtgct ttataacgcc atagattcat aaaaaaatga ttgaacccct
    42541 ttcgtttttt catctaaaaa cccctttcga ttcattcgca acgtaattat agcgctttca
    42601 ataatgagaa taaataaaca aagtataggg aaaatgaaac aaagtatagg agaggttgtg
    42661 aaatcagcgt tttggcctga gtattagaga aaaaaacggg aaaatagttc ctcatatttt
    42721 gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttatc agaggttgtg
    42781 aattcagcgt tttggcctga gtattaggga gatcgtgtcc ttactatcta actctgagtc
    42841 acaaggaaca atggggactg taacaaaagt cttgtcacag tcccttattc cgaacaaacg
    42901 gtgttccaga agtagcttct acggaaataa gccgaaattt cacaaaaaag ccatccgaaa
    42961 gtcagacgga caagtctgct tttcagatgg cgctacaact actctgctta ttcttttagc
    43021 tatccattca attatttttc tctcacttgt tctagctctt ctttggtcat gggtcgatat
    43081 tgccctttat tcaaatcttt aggcagccaa aattttccca tacggattct ttttaaatat
    43141 aagacttctt ttcctactgc tttgaccatt cgcttaactt ggtgaaactt tccttcatgc
    43201 aaaatcagac ggataattga agtttctgtc tcttgatcta cttgatcaat ttggagtttt
    43261 gccggcaatg tctgctcatc gccatcaata atcagtcctt ctgcaaataa acggatatct
    43321 tcttctgtca ttataccgct aacctcagct agatattctt tctccacatg ttttttaggt
    43381 gatagtaact gatgggcaaa caccccgtca ttcgttaaaa tcaagaggcc ctctgtgtct
    43441 ttatctaatc ggccgactgg aaaaagatct tcccgataat cctggtcttc caataaatcg
    43501 attacggttt gatcatgatt atcctgagta gcagaaatga ctcctgcagg tttatgcaaa
    43561 atgtagtaaa aatctttttg atacgtaatg atttctccta aataagtaac cgcatcttcg
    43621 aattcattta cttgcgcttt atcgcttttc accacttgtc cattgacttc gatcgttcct
    43681 tttttgatta atcctttgac ttctttgcgg ctgccaagtc ccatatctgc taaaaactta
    43741 tctaaacgca tgcgtttccc ccatttcaaa agagcatcaa tctagctctt ctgattcact
    43801 tattttattt ttaatttatg tcgtaaacgc gctattcctg gtcctagtag tctttctgcc
    43861 aagcggattt tcaaagctaa ataaccatac actaaagccc ctatacctgc tactacaaga
    43921 atcaacaata gagactggaa tttgctatct tcgtttaaaa aagcaccaaa aatcatcttt
    43981 gtgatccacg aagccaaaag cataatcaag gtaagaataa agatcagcaa tgtcctgcgc
    44041 cagacaaaac ttggtttaaa gcgggctact tcatggattc tctttaagat caaggtacaa
    44101 gacacagcaa atccgatcat tgtcgccaat aaagggccgt atacttcaaa taaacgaatg
    44161 gctgggtatt gaagaaggag tttcactaga aacccaataa ccaaataatt aattgccgcc
    44221 ttgttttcaa acattccttg cagcatgttg gaaactagca tatatagtcc taggaagagt
    44281 cctacaaaac ttgcttggat cagtacatta cttcctagag aatccggtgt ataaaataat
    44341 gtatttagcg gataggcaag taacataaca ccaaatgtcg ccggaaacat aaaaaatgaa
    44401 aacaactgta aatttccgct tgtcaatttt gccagaccta ctcgatcttt caacgtaaca
    44461 gcttctgtaa tcaacggtaa cccagtagac gcgatcgatg ttgccaatgc aataactacc
    44521 atcgtcaact tatctggatt ggcactaaaa atcgaaaaaa gatccagtaa ctgtgcattg
    44581 gaatactcgg tggatgcaga catgatcttc ataaaagtga actgatcgac taatttgaag
    44641 atcgttaccc ctgaacccac aataataaac ggaatcgctt ctttaacggt atctaacaac
    44701 aattccttcg ttgcaatatt tattttgttt tcgctgtagt gaataaacgc cgatgtatac
    44761 gcttgatgtt tcttcaaaaa atacaaaagg acaagaatac ttgctagcat accgataaat
    44821 gccgcgaaag ttgattgcgt gaccgccgtc acatagtcac catccatgac tttcatgatg
    44881 atgaatgtag ccaatagcat ataaaaaata cgtgccacct gttctacaat ctgcgacagc
    44941 gcataaggca tcatttcttg attgccttgc aaataccctc gtatcacact catacaagga
    45001 aaaaccaata cagcgatact caaagaacgc atcgttggaa tcagttcttc tccgccacca
    45061 gaagcatgag ctagccaagg agaggcgatg tacatgaaca aagcaaacaa caggcctaac
    45121 ccgcccatca tctgtaatgc acggataaat aatcgtcgtg aagtgccata ttcattcaat
    45181 gaattatagc gtgcagtttg ttttgcaatc gcagcaggta ttcctgctgt agagatcagt
    45241 agaaacaatg catatatatt gtatcccata ttgaataatc cattggcagc cttagcattt
    45301 tcgcccatcc aagcatacca gggaatgata tagacagcgc ctagcaatct tgaaatgata
    45361 ttactggcgg tcatccaagc agagcctcgt gccatttttt cttgcggtgt taattgatct
    45421 aatgctggtt ttctttgatt atccatcggt cagattccaa ctttctacaa aacattacta
    45481 tcattttaat tggtaaaaca gaaactggca atcgatttta tataactttt ataagaacaa
    45541 ttgttttttt gaattcggtt tcgttttttt ggtaaagtaa agaaatatac agccttttat
    45601 ggctaacatt tataggagat atcaatgaca caagaaataa aaaaactacc cgttatattt
    45661 ttaggtcttt tctttatcac agttggattg aattggttct tgctccctca cgatatagct
    45721 tccgccggag tgggcgccat cggacattta gttgaaacgg ccttttctat ccaacgctca
    45781 gttactgttt ggagcatcaa tcttatgatg ctgcttctag ccgctgtcct attaggaaaa
    45841 tcagtatttt ttaagactgt agcgggaagt ctgctttttc ctgttgtttt agagattgtt
    45901 ccaaaagtgg aaatgatctc ctcacatttc ttttcactga tttcaggaag tcttttgttc
    45961 agtcttgggg tttacacatt gtacacagtc ggggcctcaa atggcggtat aacgataccg
    46021 ccgattattt tccacaaatt ttttcggctt ccaatgccgc aaggactttt acttaccaat
    46081 agtcttatcg tctttttgaa ttattatgtt tttggattgt tggagacact attcgttctc
    46141 ctatctatta cagtcagttc attgtttatg aaaatactca tccgcttgtc ccctgtgtca
    46201 agaaaatttg aataaagctg gattttgtca tagcggaaac aaacgatata ttaaaaagag
    46261 tgtgctataa taaaccaaga aattgaataa agcgagggat caatcaatga gcttctcttt
    46321 agaagacatc cgtcatttac tattaaaaga aaatctatta aaagaatttg tttcaacaag
    46381 aggctggcat cttgatgtac cagaaaaagc tgaatttctt cagttaagtt atgattcaag
    46441 aaaagctgat gcttctactt tgtttttctg taaaggaatg aatttcaaag aagaatattt
    46501 ggattcagca atcgaacagg gtattcaata ttatgtatca gaacaaccat acgaaaattc
    46561 agcagtagga atcatcgtaa cggatatccg taaagcaatg gcgcttcttg ctatggcttt
    46621 ttacgactat ccgcaaaata aattgaaagt gatcggattc actggaacaa aaggtaaaac
    46681 aactgctgcg tactttacaa aagcaatctt ggatcacaca acaaataaaa aaacagcatt
    46741 gctttctact atgaatacaa cgttagatgg caaaacatat ttcaaatccc atttgacaac
    46801 gcctgagtca ttagatttat atcgtatgat gtcagaagca gttgaaaatg gtatgactca
    46861 tctagtgatg gaagtttctt cacaagctta caaaacacaa cgcgtatacg gcttgacttt
    46921 agatgtcgga atcttcttga atatctcgcc tgaccatatc agtccgatcg aacatccgac
    46981 atttgatgac tatttctatt gcaagcgaca attgatcttg aattcaaaaa cagttgtttt
    47041 aaatcatgaa agtgactatt ttgatttact tagggaaaca gccgatctat tccagattcc
    47101 aacgatcact tatggtcgtt cggaagaaag caattatcaa gtgatccgtt cagataaagg
    47161 tactcacggt tttactttat cttctcatga agatcgctta gctatctgcg atactgctta
    47221 tgatattttg ctggctggag gattcaacca agaaaatgca gccagtgcga tcattgccgc
    47281 agcactaaca ggtgcctcca gtcaagatgc acaagacgga ttaaaagaag ctcgtgtacc
    47341 aggccgaatg gatcaattga tccaatccaa tggagcgcat gtctatgtgg actatgcaca
    47401 taattatcta agcttgaaaa cattgctgaa atttgccaaa aatgaacatc cagatggccg
    47461 tgtaattgtt gtattaggca gcccaggaaa caaagcaatc tcccgtcgac atgattttgg
    47521 aaaagtgcta tcagaaacag ctgacatcgc ctttttgacg gcagatgacc cagcatttga
    47581 agatccgaaa aaaatcgcgg aagaaatcaa tgaagcaatt actaatccag atttgatcgt
    47641 tcattatgaa atggatcgtc ctgaagcgat tcgacgcgct ttagcagaaa gcgctccgca
    47701 agattcagtc gttattgcgg gaaaaggcgt ggatccctat caaaaaatca atggtgtaga
    47761 tgaaccttat gaaggggatt atgcaatcgt gaagcggttg attgaagaat aatggtattt
    47821 atatactagt ggtttcccat ttctaagaaa aaaagggact gtgatttttt gtcacagttc
    47881 cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct tttctctttc
    47941 ttcatttaat aatttattat catagaattt gataaatggg aaccagataa tgaatgcgat
    48001 gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac caacaaatgc
    48061 tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga cgatttccag
    48121 ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa acggaaccgc
    48181 taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga tattgaataa
    48241 tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg cgaggaatgc
    48301 aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat aagaattgtt
    48361 gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat tagctaatac
    48421 gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga tccacaatgc
    48481 atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact gcgtaacaaa
    48541 cccaaatggg atagcaatca ttttgaagat atccgttcct agtgcaacta ataatccatt
    48601 gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga aagaacgaga
    48661 aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga cacacataca
    48721 atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg aagttcctaa
    48781 acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct cattgataga
    48841 agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca taaagaatgc
    48901 aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt catctcgttc
    48961 aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa tccccatcgt
    49021 cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat taaaaaatcc
    49081 ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg agcctacaat
    49141 cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt taagttgcgc
    49201 aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta gttattctcc
    49261 tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg gtcttggttg
    49321 agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc gcttacaaca
    49381 agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata caagaatgta
    49441 aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg gaacaaaaaa
    49501 taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac ttgttctcct
    49561 actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa acttttagct
    49621 gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt caggtaacgt
    49681 acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt ttctaattcg
    49741 attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt tttttgatac
    49801 aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag atgatcttgt
    49861 gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc tttcagaagt
    49921 tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa cttctcttct
    49981 acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag tgtccgtgca
    50041 tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact gtttattttt
    50101 tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct gaaattctca
    50161 aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa cctaacgatt
    50221 gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac taaaaaaaca
    50281 aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc aacggctacc
    50341 tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc actgaatatg
    50401 atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat atatccttct
    50461 tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt tttcggttcg
    50521 tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg ctttttttgc
    50581 tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga ttctccaact
    50641 acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg taaaactgga
    50701 attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat tccttttaaa
    50761 gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt tttaccgaat
    50821 tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc acagataatc
    50881 aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag atgcaaagcc
    50941 aaataggccc attcatcttc attgatggta tagtttttca aagccggcat gttgctgaaa
    51001 tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc agtcaatggg
    51061 ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg atcgagtagt
    51121 ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga caatcttttc
    51181 agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt gtgattcgac
    51241 ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga tacacctgcg
    51301 attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg cttagaaact
    51361 tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt gatccccaaa
    51421 gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt agcttctctt
    51481 gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa aaaacggctt
    51541 tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt atccatgata
    51601 aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg cttagaaacg
    51661 atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt tgtcaaactg
    51721 gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc atcttccaaa
    51781 agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc ttgcggttct
    51841 ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt cagtctttgt
    51901 aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc gtttttttct
    51961 actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa taacaattca
    52021 gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata tctcaacagc
    52081 aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc tcttcgatca
    52141 cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa gggatactaa
    52201 caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac atcgtcgttt
    52261 gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg ccttctgtag
    52321 ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc gttgtctttg
    52381 aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt ttcataaatc
    52441 aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct attcaaaata
    52501 tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata atttctggca
    52561 ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat cacagtgaat
    52621 attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat ggtcgaaaga
    52681 aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta ttactttcca
    52741 ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga agcaatcgaa
    52801 aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa gtataaatta
    52861 aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag atacacatgg aatgatagca
    52921 atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct catctaatga
    52981 ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc aatatacatg
    53041 attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc tcctggaacc
    53101 ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt tttatatcta
    53161 taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt aaaaggaata
    53221 aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat tcggtctact
    53281 gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga agaaaatgca
    53341 aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat caaaagacta
    53401 atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc tttcaaaaaa
    53461 atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta aaggagaact
    53521 gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa attcttgttc
    53581 aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta caaagaagca
    53641 ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa aataaaatga
    53701 atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac taatagtgag
    53761 aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa gtcaacaaaa
    53821 cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt cgattcaaat
    53881 aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa tgatcgtaaa
    53941 aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg tattttttgt
    54001 tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctgttg tcgtcctttc
    54061 tttttacttt tattttttac agtcatctat gacaggttgt aaatactatg gaacatcata
    54121 tggcgttgtc attttcttgt caactatttt tcctattcat gtttttataa aaaaacatat
    54181 cgttttcttg tatgattaaa tcaccacaaa ataaacaact gagagaacga tatgctttat
    54241 gaatggttct atcaaaaaaa ttctgagatc aatagacaaa aatttgatga ttacagaggt
    54301 ctcttacgaa accttacaga agaaaaaaac attgatcgtt gctgcagttc tcttccctgc
    54361 tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat ggaaatagga aattaatggt
    54421 ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa taatataatc atatgccaat
    54481 ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc attggaacgc
    54541 tcaaagttat tttatccgtc caaggcactc gatttcaaat catgtcagac ataaaatcac
    54601 ttctttactg actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt aggtatcttt
    54661 gacaaccgtt attcgcacat tgaaagagtt gagaagctat ttaccaaagt cttccaaaag
    54721 aattctgtct tgagtgttga gggttgtgaa tttcgttctc acgtttccgt tgaggataaa
    54781 ctgagtttta tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat ttacctacac
    54841 aaaaaattcc tcgattaaca aactatttcc ttagctgcac taatcctaaa gaaatagaat
    54901 tcttagttac agacatgaac gccacctact ttcagctcac caaacatgtt ctaccaaatg
    54961 cgaaattggt gattgatcga ttacttggtt tttccacgcc tttcaaggag gcgtaacctt
    55021 tattcacgag ttggttgaag ctttccgaga caaaagaccc gacttgtttt tctccttatt
    55081 gacaaagagc cattaaaact agagaaaaaa ttacaactga tcgctgatta tttgtagaaa
    55141 gaaaatcaaa gtattaccca ataaacttga gtcattaggc aaaaaacact gcgaaactca
    55201 agaatgattt tcgcagtgtt tttgtatatt ttttattaac tagtattact acaaaggttt
    55261 atttttcact tctcaacgca gcacaaaatt gtctttactt atttactggt tgggttcaac
    55321 cttctctact ccgatactaa tatttgtttg tatacccctt ctaaaatcgg tagtttccat
    55381 tcaattgatg ggtactagtt gattttaaaa actatgctta ttcaattttt tattttagct
    55441 catatatttc gtttctttct aaattttata ctgatgaaaa caagtagcac acccaatatt
    55501 gacaatataa actggctaat gatggtttct ccagtttttg gaagatgctt atttccactg
    55561 cttgttggat tgtttcctct tatatctgag ctatcagcaa cttttctatt ctcttttgtt
    55621 aaaaagatcg ccacttcttg ttctttttcg atcttgaaag ttactggtgt cgcatccaat
    55681 tcataccccg ttggcgcttg agtttctacc agctgatatg ctcctggtgc taagccatct
    55741 atcgccagtt ttccatctgc tcctgtggtt agacctgact gcagaacggt tccattggca
    55801 tcttgtaact caaaaacagc tccttgcagg acttctccgc tttggtcatc gatctttgtc
    55861 aacacgacac cgcctggtgt aagtcgattt tctttcgtca attctaccac cgctgtctga
    55921 cttctttcga tctcaaactc gattggtgtc gcatccaatt cataccccgt tggcgcttgg
    55981 gtttctacca gctgatacac tcctggtgct aagccgtcaa tcgccagttt tccatctgct
    56041 cctgtggtta gacctgactg cagaacggtt ccattggcat cttgtaactc aaaaacagct
    56101 ccttgcagga cttccccgct ttggtcatcg atctttgtca acacgacacc gcctggtgta
    56161 agtcggtttg ctttcgtcaa ttctaccacc gctgtctgac ttctttcgat ctcaaactcg
    56221 atcggtgtcg catctaattc ataccccgtt ggcgcttgag tttctaccag ctgatatg
//
LOCUS       DAHHPA010000007        51641 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.007, whole genome shotgun sequence.
ACCESSION   DAHHPA010000007 DAHHPA010000000
VERSION     DAHHPA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.007"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            628..1161
                     /locus_tag="NBH55_000373"
     CDS             628..1161
                     /locus_tag="NBH55_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCD1508389.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1651..2175)
                     /locus_tag="NBH55_000374"
     CDS             complement(1651..2175)
                     /locus_tag="NBH55_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1508390.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(2168..2641)
                     /gene="tsaE"
                     /locus_tag="NBH55_000375"
     CDS             complement(2168..2641)
                     /gene="tsaE"
                     /locus_tag="NBH55_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCD1508391.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2764..3744)
                     /gene="pta"
                     /locus_tag="NBH55_000376"
     CDS             complement(2764..3744)
                     /gene="pta"
                     /locus_tag="NBH55_000376"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCD1508392.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3897..4577)
                     /locus_tag="NBH55_000377"
     CDS             complement(3897..4577)
                     /locus_tag="NBH55_000377"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCD1508393.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4703..5572
                     /locus_tag="NBH55_000378"
     CDS             4703..5572
                     /locus_tag="NBH55_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1508394.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5598..6113
                     /locus_tag="NBH55_000379"
     CDS             5598..6113
                     /locus_tag="NBH55_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508395.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(6160..6753)
                     /locus_tag="NBH55_000380"
     CDS             complement(6160..6753)
                     /locus_tag="NBH55_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1508396.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6778..6957)
                     /locus_tag="NBH55_000381"
     CDS             complement(6778..6957)
                     /locus_tag="NBH55_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508397.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(7034..7543)
                     /locus_tag="NBH55_000382"
     CDS             complement(7034..7543)
                     /locus_tag="NBH55_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508398.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7639..8019)
                     /locus_tag="NBH55_000383"
     CDS             complement(7639..8019)
                     /locus_tag="NBH55_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1508399.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8349..9098
                     /locus_tag="NBH55_000384"
     CDS             8349..9098
                     /locus_tag="NBH55_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCD1508400.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9282..10559)
                     /locus_tag="NBH55_000385"
     CDS             complement(9282..10559)
                     /locus_tag="NBH55_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508401.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10638..11282)
                     /gene="pcp"
                     /locus_tag="NBH55_000386"
     CDS             complement(10638..11282)
                     /gene="pcp"
                     /locus_tag="NBH55_000386"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCD1508402.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11481..12425)
                     /locus_tag="NBH55_000387"
     CDS             complement(11481..12425)
                     /locus_tag="NBH55_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCD1508403.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12427..13113)
                     /locus_tag="NBH55_000388"
     CDS             complement(12427..13113)
                     /locus_tag="NBH55_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCD1508404.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13587..13907
                     /locus_tag="NBH55_000389"
     CDS             13587..13907
                     /locus_tag="NBH55_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCD1508405.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14061..14966
                     /locus_tag="NBH55_000390"
     CDS             14061..14966
                     /locus_tag="NBH55_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1508406.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14963..15736
                     /locus_tag="NBH55_000391"
     CDS             14963..15736
                     /locus_tag="NBH55_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCD1508407.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15917..17341)
                     /locus_tag="NBH55_000392"
     CDS             complement(15917..17341)
                     /locus_tag="NBH55_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1508408.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17552..17998
                     /gene="nrdI"
                     /locus_tag="NBH55_000393"
     CDS             17552..17998
                     /gene="nrdI"
                     /locus_tag="NBH55_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCD1508409.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(18063..20045)
                     /locus_tag="NBH55_000394"
     CDS             complement(18063..20045)
                     /locus_tag="NBH55_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1508410.1"
                     /translation="MAKNELTLEELAKHIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(20059..20955)
                     /gene="murQ"
                     /locus_tag="NBH55_000395"
     CDS             complement(20059..20955)
                     /gene="murQ"
                     /locus_tag="NBH55_000395"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCD1508411.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(20958..22019)
                     /locus_tag="NBH55_000396"
     CDS             complement(20958..22019)
                     /locus_tag="NBH55_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCD1508412.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(22230..22796)
                     /locus_tag="NBH55_000397"
     CDS             complement(22230..22796)
                     /locus_tag="NBH55_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD1508413.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(22824..24326)
                     /gene="eat(A)"
                     /locus_tag="NBH55_000398"
     CDS             complement(22824..24326)
                     /gene="eat(A)"
                     /locus_tag="NBH55_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCD1508414.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(24584..25705)
                     /locus_tag="NBH55_000399"
     CDS             complement(24584..25705)
                     /locus_tag="NBH55_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD1508415.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            25831..26772
                     /locus_tag="NBH55_000400"
     CDS             25831..26772
                     /locus_tag="NBH55_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCD1508416.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(26922..27818)
                     /locus_tag="NBH55_000401"
     CDS             complement(26922..27818)
                     /locus_tag="NBH55_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCD1508417.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            28087..29427
                     /locus_tag="NBH55_000402"
     CDS             28087..29427
                     /locus_tag="NBH55_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1508418.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            29645..30088
                     /locus_tag="NBH55_000403"
     CDS             29645..30088
                     /locus_tag="NBH55_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCD1508419.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(30592..32010)
                     /gene="nhaC"
                     /locus_tag="NBH55_000404"
     CDS             complement(30592..32010)
                     /gene="nhaC"
                     /locus_tag="NBH55_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCD1508420.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(32413..33636)
                     /locus_tag="NBH55_000405"
     CDS             complement(32413..33636)
                     /locus_tag="NBH55_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCD1508421.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(33667..34506)
                     /locus_tag="NBH55_000406"
     CDS             complement(33667..34506)
                     /locus_tag="NBH55_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1508422.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(34523..35203)
                     /locus_tag="NBH55_000407"
     CDS             complement(34523..35203)
                     /locus_tag="NBH55_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508423.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(35205..36278)
                     /locus_tag="NBH55_000408"
     CDS             complement(35205..36278)
                     /locus_tag="NBH55_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1508424.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(36334..36572)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(36615..36848)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(36925..37596)
                     /locus_tag="NBH55_000409"
     CDS             complement(36925..37596)
                     /locus_tag="NBH55_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCD1508425.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(37683..40361)
                     /gene="mgtA"
                     /locus_tag="NBH55_000410"
     CDS             complement(37683..40361)
                     /gene="mgtA"
                     /locus_tag="NBH55_000410"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCD1508426.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            41020..41457
                     /gene="ndk"
                     /locus_tag="NBH55_000411"
     CDS             41020..41457
                     /gene="ndk"
                     /locus_tag="NBH55_000411"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCD1508427.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(41479..42345)
                     /locus_tag="NBH55_000412"
     CDS             complement(41479..42345)
                     /locus_tag="NBH55_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1508428.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(42505..44598)
                     /locus_tag="NBH55_000413"
     CDS             complement(42505..44598)
                     /locus_tag="NBH55_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCD1508429.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(45347..45970)
                     /locus_tag="NBH55_000414"
     CDS             complement(45347..45970)
                     /locus_tag="NBH55_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508430.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(46129..47526)
                     /locus_tag="NBH55_000415"
     CDS             complement(46129..47526)
                     /locus_tag="NBH55_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD1508431.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(47558..48529)
                     /locus_tag="NBH55_000416"
     CDS             complement(47558..48529)
                     /locus_tag="NBH55_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1508432.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            48903..49328
                     /locus_tag="NBH55_000417"
     CDS             48903..49328
                     /locus_tag="NBH55_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508433.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(49816..50517)
                     /locus_tag="NBH55_000418"
     CDS             complement(49816..50517)
                     /locus_tag="NBH55_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD1508434.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(50533..50973)
                     /locus_tag="NBH55_000419"
     CDS             complement(50533..50973)
                     /locus_tag="NBH55_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCD1508435.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(50973..51548)
                     /locus_tag="NBH55_000420"
     CDS             complement(50973..51548)
                     /locus_tag="NBH55_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCD1508436.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        14931 a        10764 c         9034 g        16912 t
ORIGIN      
        1 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
       61 aatagttcct catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
      121 accgtttatt aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac
      181 aaaaatagtt cctcatattt ttcgtttggt tggacttatt accgcagatg caagcttttg
      241 aacaccgttt attgagaagt tgtgaaatca gcgtcctgac ctgagtatta gagaaacaaa
      301 tggcaaaata gttcctcata ttttgctatt ttttgagcta ataccgaggg tcagctgatt
      361 gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt
      421 ttttgaaaat ggttcctcac aactttgcat aaatttgact taataccgag aagtcatgat
      481 ccccatctaa ctccaaatca caaggaatga gttcaaaaac tagcttttct caacaaatat
      541 tacataaaaa attctatgtt ttttgttaaa tttatttgct tttttcaaaa gtatgttaca
      601 gtaatttccc aaggaggttt tacagaaatg aattttgttg ccatggattt tgaaactgcc
      661 aatcatcagc cttatagtgc ttgttctctt gcgctagtca tggtaaaaaa tagccagatc
      721 gttgatgaat tctatacatt gatacagcct gagactcctt ttttctggcg aaatgtccaa
      781 attcacggaa tccatcaaga agacgtacgc aatgcgccga aattcccaga agtctggcaa
      841 actatccaac catattttca aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat
      901 acaaaggtct tagcgggatg cttagattat tatcatcttg aacagcctaa ttatctttct
      961 ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac
     1021 actgtgtgcg aaaatttgaa catccaatta gatcatcacc atgatgcatt ggaagatagt
     1081 cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa
     1141 aaacttgtga ctataaagta aaagaatact tatgaaacac acaaagaggt cgtgagcctg
     1201 ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata gcttctcatg tttattaaat
     1261 attgtgactg gtgaccgagg agctggcagg agaacaccgt ttattggaga ggttgtgaaa
     1321 aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttca
     1381 tttggttgga cttattaccg cagatgcaag cttttggaca ccgtttacca agaggttgtg
     1441 aaatcagcgt tttgacctga gtattagaga aacaaacggc aaaatagttc ctcatatttt
     1501 gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttatt caaaaaaaat
     1561 ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt tttctgcctt ctcaaaagga
     1621 tgacagtttc tcactctttt cctatttctt ctattcaatc aaataactca ttttatatac
     1681 atctaaaaat tcacctagat ctgttcttgc ccctctaggc atgaccgctt cgatttggaa
     1741 gcccattttt cggtaaaggt gtacggctct ttcattacga acttgaacgt caagctccaa
     1801 tcgaaaaagt acacccattt ccttcgccca actgatgatt tcttctagca taagagtgcc
     1861 tagtcccatt ccccaatatt cctgcagaat actgataccg acttctccca cgtgagacaa
     1921 acgaaattga ctgtctgcct tgacagatgc tgttccaata atcgtccctt catagattgc
     1981 aagcagcaat aaattattat tgctttcgta tatataatcc aactcttctt ctaaggtttc
     2041 tggtggtaac aggagttctg cttcatccaa aactaaaaat tctgtctcct tattaacttg
     2101 tcccatcatc aaaagaatgt cttttgcatc tcctggcact gcttcccgca agatgatccc
     2161 aacttcttca ctcatgattt tcagcccatt tttctagaat tcgctgtttg aaggtttcac
     2221 tttgctcccc gtatgagatt agtttcacat accgcttttc taaatctgta tcactttttt
     2281 ctaaaatcag ttccaaataa tcctccggca gtgcttcttc taatagattt ccccattcaa
     2341 caacggaaag cccatcacct tcaaaatatt catctaaccc taaatctgct ccgctagctg
     2401 ctacccgata aatatccata tgatacaacg gaatccgacc ttgactatat tctctaataa
     2461 tcgtgtatgt cggacttttg atcatttgcg ttatccctaa tccttgggca attcctttcg
     2521 tcaacgttgt tttacccgca cctagatcac cagtcaatat cagattgtca cctggcattg
     2581 cggaatgacc aattgcctct gcgaattgtt ctgtcattgt taatcctgat agttcgatca
     2641 tttttcatct tctcccgctc ttctttcttt ttgccagtat atcgcgtaat gaagtttttt
     2701 taaagaaaaa atgaactagt caagaacagc agctgctgtt tttggctagt tcgcgtgtat
     2761 ttgttaatca atcaatgttt gtgctgctgt gataatcgat aatttatata catcttcttc
     2821 atttgctcca cgagacaaat cagaaaccgg tttgttcaag ccttgtagaa ttggtccgat
     2881 tgcttcaaag ttaccgaaac gctgagcaat tttatagccg atatttcctg actgcaattc
     2941 tggaaataca aagactgttg cttgaccagc tacttctgaa tctggcgctt tcagttgtgc
     3001 aactgaagga acataagaag catcaaattg caattcgccg tcgatcatgt attgtggcgc
     3061 tagtttttta gcgattttag ttgcttcaac tactttgtct acttctggcg atttcgctga
     3121 gccttttgtc gagaagctca tcattgctac ttttggttcg atatcgaaca attctgctgt
     3181 ttttgcagaa tctacggcga tttcagccaa ttcttgcgca gttggattca cgttgatcgc
     3241 acaatccgaa aagacatatt tttcttgatc tcttccacgt accatgatca tcgctccgct
     3301 tgtccgactg atgccaggct ttgttttgat gatctgaagt gctgggcgaa cagtgtcgcc
     3361 agtcgaatgg atcgctccag agaccattcc gtctgcaatc cccatatacg taagcatcgt
     3421 accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt tctttggatg cctttccatt
     3481 tctacgttca acaaaagtat ccaccatttc atcccactta tcgtaattat cagggctgat
     3541 gatcgtaaag ctagatgttt tgattcctct ggcatgcgct gcatttctta tttcttcttc
     3601 gttccctact aaaatgggtt ccacaagttc ttctgctttc aaccgggcag ctgcgcctaa
     3661 aatacgagca tcagtagctt ccgggaaaac gattttgatc ccacggcgaa caattttaaa
     3721 tcttaaacta tcaaacaatt ccacaagatg acctccagac gtttttatat gtattatcat
     3781 acactattcc agaaaaaaag aacagtttat tttatctgta tcagtcaaaa atgctggaaa
     3841 aattaagttt ttcacataca aagcgaactg cactataaca ggaaagcgct gttttattac
     3901 actgtttcag gcagttgcca atcaatcggt tcttctccta acgcaaggag cgcatcattc
     3961 gttttcgaga agggtttgga accaaaaaag ccacgacttg cagataacgg actgggatgt
     4021 ggagatgtta tgatgatatg gcgactctta tcgatcattt tgattttttc ttgtgccggt
     4081 tttccccaca agataaacac gattggtttt tcccgttcat ttaatttttc aatgatcgtg
     4141 tctgttaatc tttcccaacc tttcccgcga tgagaatacg cttgcccttc ccgaacggta
     4201 agaacggtgt tgagtaaaag gactccctgt ttagcccatg acactagatt tccatgttga
     4261 acaggtgcga ttccaagatc atcataaagc tctttataga tatttcttaa cgatggcggg
     4321 atctttacgc caggttgcac agaaaaagat aagccatgag cttgattttc tccatgataa
     4381 ggatcttgtc ctaaaatcac cactttaact tcttcataag gagtcaattc caatgcttca
     4441 tagatatgat acatatctgg atggatcttt tgggacgcat attctttttt taaaaattct
     4501 cttagatgca gataataatc ttttgaaaat tctgaggaca aaacttcttg ccaactgtta
     4561 tgaatgattt ctttcaaaaa ctccacctcc taataaaaag aataactcac attttcttca
     4621 atcacaaggg acaatcttca ccctcagcac ttgattaata tggtaaacta ggaacaagaa
     4681 gcagaaagga acgataatca ttatgatcaa attaattgca tcagatatgg acggcacatt
     4741 attagatgcc catatgagtg tttctcctga aaatgccgag gcgatacgtt ttgcaaatga
     4801 tgccggtgtt gagttcatgg tcgctactgg tcgaaactat caagaagctc gcgcagcttt
     4861 agatgaggta ggaatcgatt gtgcgatgat cactttgaac ggggcacagg tatttgataa
     4921 agaaggaaat tcattgttta ccgtgtcgat tccaaataat caagcaatct ctgttttaga
     4981 tattctagat gctaacggta tttactatga agtagcaacc aacgatggtc tttattctga
     5041 aaatcaagcg aaacgaattg aaagttttgc ttccatggtg gctacccatt tacctcacct
     5101 tacgtataaa atggcgattg ccatggcctc agctaagctg gagttattgc acatcacata
     5161 cgtggacagt attcgtgcta ttttggaaga aaagaatctg gaagttttaa aaattatttg
     5221 ttttcataca gaagggccag ctgtattagg tccagttgga aagcagattt ctcttttagg
     5281 cgatctagcc gttacttcct ctggtcaaaa caatatcgaa gtgaaccata aaaacgctca
     5341 aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg atttctttag aagaagtcat
     5401 gacgattggc gataacttca atgatgtcag catgctgcaa gtagcaggag tcagtttcgc
     5461 aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa tatacaacgg acacaaacct
     5521 tgaatctggc gtaggaaaag ccatattgcg cgcgattgca gaagacttgt agttttatct
     5581 agaaaagagg gatatagatg tcagaatttt tcattcagga aaagcagctt ggacgtgcaa
     5641 ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt tttgatggtt ggtcgctggg
     5701 gaacaaaggg cgatgcattg tctctttatg ccatgaatgg cgatctcgtt gctcacatca
     5761 agcagataag cctgacattc ggtacgcgtt ttgagctgta taaagagttc gaaaaggtgg
     5821 gaaccatgca gaaaatattc aattggcccg gtgactttta ttatattcgt agacttcact
     5881 ggacagtcca aggtgatatc tataatcatc aatatcaaat ccatcatttc aatcagcaaa
     5941 tcatgaaaat ggataaagca acactttttt caggagatta ctatgtattg gatgtctcaa
     6001 atgatgaaga tgctccagtt tgtatttgta ttgctgcggt tttagattac tggctctacc
     6061 gtaaagacaa aagtcgcgat aagcctgttt cttaccgact taacttaagt taaaaagatg
     6121 tacctgaaat gcggaaatac gcatttcagg tacatctttt tagatattct tttgcatatg
     6181 atcataaaga tgaccactga tcatccgctg accgacaact ttgaagtcat ggcgttcgta
     6241 taatttttta gcctttggat ttttttcatc cacacttaga ccgatgacgg agcgattagc
     6301 tttcttcgcc aatttaggta atgcttctaa taatcgcgaa ccgatgcctt gtcctctaaa
     6361 atcttctgat acactgatag agtccaaata ccattcgttg ggaaacgttt ctttatcaat
     6421 gaacaaacgt gtagacgcat ctagctgata tttctctaag ataggtacca gtggctgatc
     6481 gatgattgat tcttcttcat ctgtataacc gtaagcaact cctgcaactt taccgtctat
     6541 ttcttctacg atacctcgtg aataactgta acgataattt ggatcagtga ttgcttcttc
     6601 taaaatctct aaagtctttt gttcccctgt ctgcttcaaa aatggcagtt ccatatcttt
     6661 tagaatcact aagatcaatg gggcgatttc tggagcatct tcttttctcg caaaacgaat
     6721 catagtttcc tctccttttc tccattctaa catgcagaaa aagaaatcgc aaaatcgtta
     6781 ttttaaaaaa tcttcaatct tttcagtaat gaaataacca tgagtaacga ttcttgccgc
     6841 ttcagttcgg caataagggc agacgactgg catttcttta tcattatgtt cgactacatt
     6901 attgacttct tcgtatgcaa atttttttcc gcaattaggg catttcattt tttccatgtt
     6961 atctcaactt cctttcatct ttactctctt agcttactct tatattgtaa aaaagagaag
     7021 caatatcctt cttctatgca gatatccata cctgacggtc ctgttctcca tctattaatc
     7081 ggactttata ataaggacgt tcatctttga ctagaagtgt ccactctttc acttttgctt
     7141 ttttcccgta gtagctgact gcttccttca tcgctgcttg cggttgtttg aactcttccg
     7201 ttttttctat atcaaaaaga tgttctgcgg ccttttccgt gtcatttcgt acggttgttt
     7261 tcccagtgga tggattgact gctatgactt gtccttcaca aaaaaactgg tattcatatg
     7321 ttgcattcgt aggattctcc aaatcttttt caactgcaaa atgaatagca gatatgttat
     7381 gttcttttgt ttcgtttttt acgattgctg ctgctttttt caatgagatc cgatattcct
     7441 ggtcactaat atcatccatg tgaaaagcgg cattgagcag tcccgtcata ccggtaatcc
     7501 ctattacgat gagggcagca acaccaaaca tcattttttt cattcttcat tctcctgttt
     7561 ctttttttta aagaatatca agaaatgaac gaatgaaaaa tcgaatgaaa cgaaaaaaaa
     7621 gtgacaattt tgttatcttt attcgtgcag ttctaatggc aatccgtctg gatcaaagaa
     7681 aaacgtcatt ttttttcctg taaaggtatc ttctctaatc ggttctgatt cgatccctaa
     7741 ggactgcaat tctgcaattg tttcttcaat atcgtctact ttgaaagcaa gatgacgcaa
     7801 gccacaagct tctggatagt ttggtcgttg tggcgcatta gcaattccaa aaatctccaa
     7861 ttcagcactt cctagtttca gatctaattt atagtcatct cgttctttgc ggtaattttc
     7921 tctgattact tcaaatccta atttattaac ataaaaatca cgtgatttct gataatctga
     7981 aacaatgatg gctatatgat ggatcgttgt aaggttcata tgagcactcc tattcttcgt
     8041 ttaagctcgg atctatatta atctccagtt ccattgtaaa tgtgtcagat tgaaaaaaat
     8101 agctgttttt cctttttata agaaaaaagg caaaaaaaac agaaagtagg tcctccaaga
     8161 cactcttcct gttcccaata taataaatga aagtaagaaa ttatattttc aagaaaacaa
     8221 acagaaaatt atcataacac aaattattta taattgaaag actagttttt tttcaagaaa
     8281 agcatacaat agattgtgtt agtaaataca gaacacacta catttagtgt cttaaggagt
     8341 cctcatacat gaagtcatat tttcagtttt tagctcatca gctaaaaggc tggccgcaac
     8401 aaaattatta tcttttcttt ttcagtcttg gctgccaggt catgactctt gtcaatagtc
     8461 ccatcacttg gctgtccgtt attacattta tcggtaccac gcttggtgta ttgtgtattt
     8521 tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg tattttgtct gcgatttgtt
     8581 ttattatcgt tgggttttca gcaaagaact atttaagtat cggtgaacag attgcttata
     8641 tgatcacatt ggatatcccc gtattgttaa gtaaagaatg gaatcataat atggcttcaa
     8701 aaatccgaaa attcactgga aaaagctggt ttatcgcaat catcagtact ttccttgtct
     8761 atcttgtttc aggattcctg attggtcgtt atacagatga tcctcggccg tgggtagatg
     8821 ccattgcttt ttctattagc ttgactgctg gtatcatcag tttcttgcgt tacaacaacc
     8881 aatattactg gtggttagct tcagggcttg ctcaaatggt cctttggtat atttcattta
     8941 cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc tgtttacttg ctgaatgatg
     9001 tccttgcatt taccgtttca ccttggtata accaaaaaga acgtgaacgt atgcaaaaaa
     9061 tggaagaaga atactacaac gcacaacaac acgcataaaa aaactgagcg tgggacaaaa
     9121 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaatc agaagcaact
     9181 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct
     9241 tatttcttgg agttaaacgc ttctgtccca gcctctattt tttatagtaa ttccttcaga
     9301 tactttcttg cctcatcatt gaaacgcagt gattcttcta acttctgata atattccaac
     9361 gcttgtttct gagcttcagt aaaacgataa tgttttcctt tccatttgtc gattgtctct
     9421 tgcacatttc cagattggat atctttcact actagttgcc atttttcttt tgagactgta
     9481 cggtctttct tatcagcaga ttgtatagct tctcgttctt tacgcaatac ttttaaaaaa
     9541 ccatttctga ctatccgagg aatgctgtcg aagaaccctt ctgtaaaacc atagcgacaa
     9601 tattgcttcc aaataaatag atagattttt ggttggatga atcgttggtt tttcgcaaaa
     9661 tattccatct gagcagtgac ttctggtgtt cgttgacgtt ttgtctttct caacacggaa
     9721 ataaatgttt caaaataagc attaggatgt actggcactt cttcatatac gatggattgc
     9781 caatctttac gtaaaaatgc gcgatagttg atcaagaatt cttctacatc ctttactaat
     9841 tcttctgcaa agtcgtcaaa tagttctttt tttatctgtc gcttttttga ctgcccattg
     9901 atgtgttcac ttattttctt gatttcattt tccaatgctt gataatccgc tatgtaaatc
     9961 ggcatttcta cttggcgaaa ttctgcgagc cgctgagcta attttatatc cggtaaatat
    10021 ggctgtttca tccgacgatt ttggtacaag acaaaacagt atttttgcca tagtccttca
    10081 ggaaaatcga tattcttctg cttaagccat aataattcgt ctaaggccag atcaactttt
    10141 gtcattccct gatggatcga agctgccact gtcggagaga catgcaggtg atcggcaaag
    10201 tggttgatgc ctagttttag gatctttccg gtttttggcg atttaacgat gtattgcact
    10261 tttaattctc taccacactc acaatacaat ttttgctggc ttcgttttgt tcgaaaaaac
    10321 ggattgatct tgaagtcaga aaacacccaa tctgttgcag ctaaataatg ttcttccata
    10381 aaacgagagc tgattagata tcggcggtgc ttatcgacta gttcccgctg ttttttcgtt
    10441 aattttgcat agatctccca acgttgactg cttgtgtatt gtttagaaat tttgatggga
    10501 tagcttttgc tgatttcttt tggaaaccat tcttcaaaga cggattgcca ttcttccacg
    10561 gctgtttctt tcctttctaa ttacaaaaag ctggctacta acataacgtt agcaaccagc
    10621 cagtgtttct tgctaaatta gtgagttgtt ccggcatttc cggaaatatc ttctttattt
    10681 tctactgctg cttcaactgc ataagtcaaa gaagaagcaa tgtcttctaa agacatagaa
    10741 ggcgtaccca ctggacgatc gatcacttga tcgggtgaga atggcacgtg gatgaaccca
    10801 cctcgaattg ccgggaattg tgtttcgatc aaatgaagca aacgatacat gatatcattg
    10861 cagacaaatg ttcctgctgt ataagagatg tacgctggta gaccatgatc ttgtacattt
    10921 tttgtcattg ctttgattgg caatgaggta aagtaagcgg ctggaccgtt ttcttctaaa
    10981 gccgtatcga atggctgatt tccttcatta tcagggattc ttgcttctgc tagattgatt
    11041 gcgacacgtt cgaacgagac tgctgaccgc ccacctgctt gtccgacaca gatcacgaca
    11101 tctggttggt gctgttggat ggcttctttg actacttgac tgcttttttc gaatacggtc
    11161 ggtacttcca ctttgatgat ttcagcaccg ctgatttcat ctggcatttt ttttactgct
    11221 tcataggctg gattcacttt gtccccacca aatggatcaa aacctgttac tagtactttc
    11281 atctgttgtt cctccgtccg attgatttaa catatggtta tcttttatca taccattttc
    11341 cttgaacagc gtaaataaag gaaacaatag tttaaataac ataacagatc aaacttaata
    11401 tattcttata aaaaaatagt gttccactac aatgttagaa gaggttgcga caattattct
    11461 gtcacaaccc ctctcttcta ttatttaaat agaatcatat aagcaatttg gaaaactagc
    11521 ataaataacg caataaacaa ctggtttttg atcactccgt atttgtcttt catctccagc
    11581 atcgctacag gcacgatatt gaagttagca gccatcggtg tcagcagggt cccacaaaaa
    11641 ccgcaagtca atgcaaccat tcctattaac gctggattcg ctccatgact aaatacgaat
    11701 ggtgcaccga tcccaaccgt catcacggtg atggccgcaa atgcattccc catgattacc
    11761 gtaaatatca ccatgccaag agcgtaaatg atgatgccga tgttcacatt tccttctgga
    11821 acgatcgtac ctactgcatt ggaaatcact gtcccaactc cagcgcttgt aaataccgca
    11881 cctagagaag ctaaaagcat tggcaacatg cttaacggcc ctactgtgcc cagcatatct
    11941 gctgcatcat ctaaaaatgt acttggtcga ttatctcgag aaaagaacat caagattagc
    12001 atggctgcaa aaacaccgac gcccacgccg actaaagcac ctaaattggt aaatagagca
    12061 aacaagatcg caaagacacc gatactcaaa gctggaataa aaattttcat tcctagtttg
    12121 tctgacattt tttccatata ttcttttgat ggaagccggc tttctccttt gctgactttc
    12181 ttagcaattg ctggtatggt catcgcaaag atcaagccac cgttcacgat tgccggaagc
    12241 catcttcctc ctgcaataac tacccctaag accgtccaga aaaaggctga cccataacga
    12301 tgaggattgg ttttatctgt taaatttctt actccagtgt aaatcaatac gagtcccatc
    12361 acgatataaa ggatctccag taatttatcg cctaataaaa tatcaggatt tgtaaaaaag
    12421 tccatcttat tgcccctttc ctggataata tttcttcgct aatttctttt ctttaagata
    12481 gaagtagaca cttgccacaa ctaaagccgt gatagccact ggaacttcca ctaaagcaag
    12541 ctgtcccaat gttacgtcat atcctagatc tttcagtgtt gattggacta gcaacgcccc
    12601 tgctcctccg atgaatagta cttggccaaa gaaccagcag acattttcca tagctgaagc
    12661 catacctttt agttcttctt catgcttagg atggacttct aacccttttg attcgatggt
    12721 ccccaatgcc atcggtaaaa tgattggacg aacaaatcca gcaactccac caaaactcac
    12781 gttaaaagcc gcaaagatca ttcgaaagat cccataaata tctacaatca taccagcaga
    12841 tactttcttg aatcgcttga tcaaggaagc cgctgattct ttcagaccat ttctttcaag
    12901 cgttcctgtt gccaacataa tgatgataaa aatcgccatc ccgcgattgt ttacaaaact
    12961 tgtacctaaa agttctaaca ttccttgaat gccaagaccg ccagtcaaag ctgtaacaac
    13021 taaagcaacc attacgatca atatcgagtc taatttaaat gcaaacccga gaataacaat
    13081 caagacgcct aaaaggctaa gataattttc catatatttt cctccataaa atgacaatat
    13141 ttttttaatt ataaagcaaa acacataaaa aatatagttg ttttctcacg aaatagacac
    13201 tatttttaaa tatttttcgt tattttttta tcacaaaaat tatcttactt attgtaaggt
    13261 aaagcttttt ttaatttatt gcccgataat atatagaaat aaggtctagt tactgttttt
    13321 tttaaatgat taataaataa caacttcttt ctatgaaatt gcaaagcatt catcttcttt
    13381 tccgctaacg atatatccgt cagtgcttgg tacactttac taaaaagtat gagattgccg
    13441 atatctggat aggtgtgcta tactacagaa aaagatatac aatctgccac cgggtaggat
    13501 aaagaggatg atcattccat taggtctttg aagggatgat tttcctcttt ttgtgtattt
    13561 ttttatagtt aaggaggaat gtaagtatgg catggatgga attaatcata gcaggaattt
    13621 tggaagtatt ctggtcaacg atgatgaaat ggagtgatgg tttttctaaa atcaattatt
    13681 ctttttacac agtgattggc atgattgcta gtttttactt tttgtcaaag gcaatcaaaa
    13741 gtttgccgat gagtctctct tatcctatct ggacagggat tggcgcagtt ggttcggtca
    13801 taattggtgt ggtcttgttt catgataagc tgaatctttc cacttggttc tttgtgggga
    13861 tgctactcat cagtatcatt ggcatcaaga ttactagcgg ccactagaca cttggaataa
    13921 gtactgatca gaaaaaataa gcctttttat ttaatattca atttttttat gaaaaaataa
    13981 gaaataagtc caatgaccta gcgacttctc cactcatcag gtgtatgctt gtactatcta
    14041 tagaaaagga gtgaatattt ttgaaaagtc tttcggtcca agaattatcc aagaactttg
    14101 gtcacaaaca agccctcagc gatgtcagtt ttagcttatc ccctggcaaa atcgttggat
    14161 tagtaggtcc taatggcgct ggaaaaacaa cgatcatgaa agctatttta ggactgatcc
    14221 attactcaga tggaacaatc tgtattggtg aaaatgagat ttctccctct agtcataaag
    14281 ggttggaaca agtaggagca ttgatcgaat acccgggtat ctatccattc ttaacaggct
    14341 atgatcatct gaagttattc tctgaaacca acgatgtatc tgctatcgac actatcgtga
    14401 atcagctcaa gatggaaaag tacattcaca aaaaagcgaa atcctactcg ttgggaatga
    14461 aacaaaaatt gggaattgct cttgccttac tgaaccatcc gtctatcgtt attttagatg
    14521 agccgatgaa cggtttggat ccacaagcaa caagagatgt gcgaaaaatc attaccgact
    14581 tggctactca gggcacctct ttcttgatct ccagtcactt actcagcgag cttgaaaaaa
    14641 tggtcgatga gctgatcctg atcaatcaag gaaagatcat caaacaatgt acaatggctg
    14701 aattgaacca aacagcgcaa gatttcatcg ttatctctac aacaaatgat ctagctgctc
    14761 gaacgatctt gacagaagct ggatatactt tggaagatcc tgataaaatc aagcttttaa
    14821 aagaaaacga agaattatta ggaaccgtga tccagctatt gtctgcacaa aatatccatg
    14881 tgacagacgt ccagcatctg acgaatgatt tggaatcttc tctattggaa attctggatt
    14941 cagtaaaggg ggaagaagaa aaatgacatt gcttgtaaaa caagaattat ttaaattgat
    15001 caagaaaaag tcaactgctg ttttatctgt tctattagtt gttttgctca taggaacagc
    15061 acttctagcc aaaaaataca cgacgatcat tgatccagtt gaaatgactg ctcagctttt
    15121 tagtgccaca tcttggattg tctttatcat gattgcagct gcaagcacga tcatttcgat
    15181 ggaagcacaa tatggtacat tgaaaaatct cctttatcga aagtattctc gaggagaaat
    15241 attagtaagc aaatggatca cattagttat ttattctgtt tatttatacc ttttagctat
    15301 tattgtcact gtgttgatga aactgattct tttcccttcg atttccttca cggagaaggt
    15361 ttctactggt caaacactga tccaaagctt gttcacttat actttgggta gttatatcgg
    15421 tttatggctg attttaagtt tagtgttgat gattgcttgc tttatcaata gttccggcgc
    15481 atccatctca gcaggaatcg tcttttattt tgcttcgtca atcatatctg gtatccttat
    15541 tgcactcatc caaaaatggg agtggatcaa atggaatccg atcagtatgt tgaatctgca
    15601 aaaccaaatt ggaaacgaag agatcatgaa gcaattgact catctttcta ctaatcaaat
    15661 gctattcggt aatttagctt acatcgtttt gttcttagca ttaggatatc tcgtatttaa
    15721 gcgaaaaaat gtatgatgac tcatcaaaaa agaggctgtg aaagagacgt tcagccttat
    15781 tgccgattgg ctgtctctta aacaccgttt attctgaaaa aggagctgtg acaacatttg
    15841 tgtcacagct cctttcctag tatgtttttg aataaacgac aggctcacaa cctctcttta
    15901 tacttctttt atacttctat acgtgaagca tcccagtggc aaataaaacc attgcaatca
    15961 ctccgaccat cgtcacacct ataagtaaaa tcttatctaa gtggatatgt tgtacaggct
    16021 cgttttgctc cttacgtgct ttgaggtaaa aataaatgcc tggcaagaaa agaacaagaa
    16081 caagcattac ttcattaaag gctacgacta gcatagccca tacttgaaag gcacttgcta
    16141 aaagccctac taaaaattgg acccattctt tccgctgata agaaagtttc atttggtaca
    16201 atcctaagaa aagccacgtg aagaaaatag ctgctgtacc aagtgtgtaa gcaaagttat
    16261 aagcactgtc agtgaataat aatgaaagta aaaagacatt cgtacataac gtcgtcaata
    16321 cgatagcata agttggtgcc ccatttttgt tgagttttcc ccatgcttta ggcaaaagtc
    16381 cgtcttcagc catgtttccc attgtttcaa tcggtaagat cgtccatgag agccaaacac
    16441 caactaaaga aatgatcaaa ccgccgttga tcacgactgc tccccattta ccaaccatca
    16501 tttcaaacac atagcccata gcaggctgcg ccttgatcgt ttgcagctga ttcatcgtca
    16561 ttgtcccata tggaagcatg gaaaccaaga cgtaaataat aatcaagccg aatatcccta
    16621 gaatcgttgc tttcccaaca tctgattgtt tttttgctcg tgaacctacg acagaggctc
    16681 cttcaatacc aacaaatacc caaagcatca ccatgatgca actcaatact tgactggaaa
    16741 acgggatcgg ctgtccattc gcattcaata taatgtcgct agaaaagtta tcaatgaatt
    16801 gttggatcga aaacgtgctg atagctacta caataaataa aaataatggc actaatttac
    16861 agaatgtgac gatagcattg acgaaactag cattttcgac tccccgattc actagccatg
    16921 catatcccca taaaaagact gtactcatca cgatcgatgc cagattttgc ccaccttcaa
    16981 atactgggaa gaaatatcct aatgcgctca tcaacaatgt tgcaaaagct acgttgccta
    17041 accagcatgt gagccagtaa ccccaaccgc tgataaaacc agcgaaacgg ccaaaacctt
    17101 cttctgcgta actaaagatc ccgcctttta aatctggacg ttttttattg atattattga
    17161 tcgacaagga gagcatcaac attgctgttc ctacgagtac ccaactgaaa atagctgggc
    17221 cgccagccga tccggagatg tccgtcataa taccaaatac gccacctcca atcgctgaac
    17281 caattactaa ggcagcaagt ggaattaaac caattccttt tttctgttgc tctgtatcca
    17341 tgtaagcatc ctccaaaata aaaccgcttt tatcaaaaag tataacaaac cttttttatt
    17401 tttacttaat taataaaaac tttaatagaa atataattct accggctttt ttctcattat
    17461 acaagcaaag cgcctagtcc ctttccaaat tacaccacca aaaaacttaa aaaagatgct
    17521 atactaaaag gaaagaattg gaggatgatc catgaacatt ttatatatat cgatttcagg
    17581 aaatacgcgt gcctttgcca aacatctagc agaatatgct gaaaaaatgc atgcagaaga
    17641 tcctgtcaat cctgaagtta ctttaaaaga aatccatgaa aatagtgatt ttacgaaaga
    17701 aacagaacca ttttttactt tcgttcctac ttatttagac ggcggaaatg gtttagataa
    17761 cggtgataca gaaatcttga ccgaaacgat gcgtgaatat ctggaacatg aagacaatca
    17821 taaactttgt cttggtgtag tcggtagcgg aaataaaaac ttcaataatc agtattgtct
    17881 aacagccaaa caatatgcac aaacatttgg ctttcctttc ttggcagatt atgaattacg
    17941 gggaacacca tctgatgtga cacgaattta tcagatttta gcagaaaaca ataaataata
    18001 aaaaagaaga agctgaaaaa tagatttcag tttcttcttt cgtttctatt gtttctatca
    18061 tattaagctt tgacatccat cacaggtgct tttgcttttg ctttacctgt tttttccaag
    18121 acaaagtttt ttacacgatc catattggtt accaagacga tcatggatgt ttctttgcct
    18181 gctgctttga tcgcttctaa gtcagcttct gctacaagcg tatcaggtgt cacttgctgc
    18241 ccttctgtta ctttcacatc aaatggcgta ccgcctaagt caactgtgtt gattcccata
    18301 tgaagcaaga tttctaaacc atttggcgtt gtgatcccaa tagcgtgttt cgttgggaag
    18361 acagagctga ctaccccagt aactggagaa tagatatgtc cgttttctgg ttctacaaag
    18421 tagccttcac ccatcatctt ttgtgcaaaa actggatcac ttgctttatc taaagaaaca
    18481 actgttccgt cagcaactga tgagaggata gtcacacttt cagctgaagt ttgaggtact
    18541 actttttcag gcacttcatt tactgtggct gtttcagtcg ctaattcttc tttttctaaa
    18601 cggtctttag gaataccgaa gaagaatgta gcaacgaagc caccagcata agctgccaat
    18661 aatccaagga tgtagcctaa ccatttaccg tcagagatca atgggatcaa agcggctcct
    18721 gaaggtccaa tagcgatagc cccgacattt ccgatcattc cgatcactgc cccgccgata
    18781 cctccaccga tacaagcagt aataaatggg cgtcctagag gtaatgttac tccgtaaatc
    18841 aatggttcgc cgattcctag gatcccgact ggtaaagctc ctttgatcat ttctgttaat
    18901 tctttatctt ttttgcagcg aatccataat gccaatgctg cacccacttg tcctgcacca
    18961 gccatcgcaa gaattggaag caatagcgta cgaccagttt catcaatcat ttgcacatgg
    19021 attggagtca aaatttgatg cagtccgaac ataaccatcg gcaagaataa tgtgcctaga
    19081 acgaagccgg caaatgctcc accaacgcca agtacccagt tgatcccacc gaccatgcca
    19141 tcagaaatga aacctgctaa aggcatgatg aagaaaattt caatcagacc gatggcaatc
    19201 aatgaaagag ttggtgtaac gatgatatct accgcatcag gtactacttt atgcagtttt
    19261 ttctcaacga ttgacaatag ccaaacagcg aagatgacac ctaggatccc accttgtcca
    19321 gctgcaagag cttcaccagt aaataagttt ttcagtggtg cttccggatt catccctgtc
    19381 agcatcacaa cggccccgat aactccacca agagttggtg ttgcaccaaa aacttgtgca
    19441 gcattgatac ctgtatagat aactaggtag ctaaacatcc cgtttttcaa gatgttcatc
    19501 acatcgatat attgttgcca tgttgctgca tcaagtgttc cggctgtgac taagtttgct
    19561 aaaactgcgg caatccctgc cacgatccca gtaccgacaa atgcaggaat cattggcaca
    19621 aagatattag aaatgtcttt taataccgat ttgattttag atggtttttg tttcgatttt
    19681 tgtgccgctt tcatttcttg tgcccgttcg tttacacggt cttttccaga tgctgatgaa
    19741 tttgctgtag ctgtacttac agaagaaagt ttttctccta atttcacgcc tgccaaatca
    19801 accatttgct gtgctacttt gttcactttc ccaggtccga tgatgacttg aagctgttca
    19861 tcttctacta caccaagcac tcctggaatc gcttttaatt cagagacatt tacaagatca
    19921 tcattgatga tcttcatacg cacacgagtc atgcagtgga caatgctttc tacattgccc
    19981 atgccgcctg cccctttata aatgtgtttt gccagttctt ccaaagtcag ttcgtttttt
    20041 gccatggtct tttccccctc ataaggtccc ttttacaaat ccgtttgctt cagctaattt
    20101 tgcttgagct tcctctttat cactatttgt caaaatcatg acgatagcca gcttaacatt
    20161 ttgatctgct tcttcaaagt acttttcagc tgtttcgtaa tcagcttctg tcgcttgcat
    20221 gatgattcgt tttgagcgtt cgattagttt ttcatttgtt ggtttgacat cgaccatcaa
    20281 gttattgaat actttaccta taccaatcat actgatagta gaaatcatat tcaagataag
    20341 tttttgtgct gttcctgact tcaatcgagt agagcctgtc aaaaattctg gacctgcatc
    20401 gacttcgatt gggaaatccg cgtgtttact gatttcggct tgtttattgc aagaaatcgt
    20461 tcctgtgaca gcaccgatcg attttgcgta atctaatcca ccgatcacat atggtgtacg
    20521 accgctagct gcgataccaa taactatatc attttttgtc aaattcagct tgattagatc
    20581 ttcttgccct agctcataag aatcttcggc cccttcgaca gctacagtca ttgcttcttc
    20641 tccaccagca attagaccta tgaccatttc cggttctaca ccgaatgtcg gtacgcattc
    20701 agcagcatct aacacaccta gtcttccact tgttccggct cccatataaa tcagacgtcc
    20761 acctttttta aaggactcaa tcgtgttagc aatcactggt tcgatacttg tcagttcttt
    20821 ttcaaccgca gcggccactt tctgatcttc tttgttcatt agttgaacgg cttgagaaat
    20881 actcatctca tctaatccaa aagtttcttg gtttctgcgt tccgttgtta ggttctctaa
    20941 attgattttg ctcattttta tcttccttct tttcttataa attgatagtt cataccagct
    21001 ttgatttggt tgatcaatgg tagctcttct ttgataactt tagctactac attcactttt
    21061 tcatccgctg gcaaatcata tttggttaat tggatctcac ccatatagcg caaatacttt
    21121 tcgttatcta aagtaataga ccctttcata cgcgtagctg tatttcttgc tgggataggc
    21181 ggaatctctc gaaaacgtgc atctacgctt cgtatgacat ctcttgcatc gtcttgccga
    21241 ttcgtatgct tccctaatat atattcgtaa aattcttcat ctacaggttc cgtatggagc
    21301 aagactgtct gttctttttg gaaagaagca aattgctcct gaacgttttc tgacagacct
    21361 gcatctccga tgtaaacaat atccgtgtta cttgctaata gatctagagc gcctgcaaga
    21421 ggatggatcc ctcgatgttc ttccaaagtt ggcaatcctt gatatagtgg gcctctcaac
    21481 tctgtatctc ctggtacaaa accttggaca aggagacctt gaattttgag ccattgattt
    21541 tttttctgat accattcttt gtccagccct gtttcgggac gtggataata attatgccaa
    21601 gcttcaatct gtgagaaatc agcttctgct tctttcaatt catcgatatc tttaggcgtg
    21661 atcgtgctag cattcaaact gattttcaat tgatgtgacc agtcggcaat ctgctgattg
    21721 gaaatatgat aatccattct taaacctgtt acaccaattg ctttcaaagg cgttagatct
    21781 tctaatgaaa aacccgctcg ttccaatgcc tctcctgaaa tatcgaccat caattctagt
    21841 ttattctttt tcgcttcgct tcctagttcg atcaatcgct tttgatatgc cgaaacatca
    21901 tcttctggta tatgcatgga agtgaaaata ccgacaaagc cattggatgc catttggcgg
    21961 atatactgtt tcttctcttc tgacaaagaa tcgttcataa atattgaaaa gccaaacata
    22021 ttatccctct ttttctgtat ccgtcttcat ttctatgact agagtatata tctaaagaaa
    22081 caaaatttca atacttttat taaaataatt aaatttcatt tctaaaggtt gagtttatat
    22141 tatcaaaaga attgtcataa cctaaaaaag gcactgctaa gcaaaagcag tgccttgaaa
    22201 tattttattt aaataacaca aaaacataat tactcataag aaaccaattc tgtattatct
    22261 accatgaatg taaagaaccc ctcattaggc atcggatgaa aataagcatt gatcactctt
    22321 atcaaggcat tatggcaaac taacagatag acattttctt cgtcttcgat gcattctttc
    22381 aataaaggaa cgatccgtgc atacacatct aagactgact ccccatttgg gaatcgtaca
    22441 gcgaacgcta aacgtgcttt ttgaaagttc tcatctttgc ttggcagtcc atcataatcc
    22501 ccaaaatcca tttctaccaa tcgttcatca acggtcagcg gcaagctgag acgatcagca
    22561 actgcttgtg ctgtatctct tgctcgttga agaggggaat ggatgatttt cgtgattgga
    22621 acaggcaaat ctactacctt ttctgctagt ttctgtgcct gcatctgtcc tttttcagtc
    22681 agcggcacat ctgcccgtcc gcaaactaat ccttgtgcgt tccaagttgt ttctccgtgt
    22741 ctcgttacat aaagcatgtg attctctcct tcgttggcaa agttcccctt aaccatacct
    22801 tgttgttctt gagattacaa gggttagatt ctctcaagag aaataatctc agtagatact
    22861 ttatccagga aggtttgatc atgttccact agtaacatgg caggtttcac gttcaagatc
    22921 agttgttcta attgttcttg attgaagaca tccaaataat tcaatggttc atcccatgta
    22981 tatagttcag ctggctgtga taaagattta gccaattcca cttttttccg ttggcccata
    23041 ctcatctgct cgatcttgtt atgaaaaaca tctctttcca tcccaagctt tcggaggttg
    23101 ttcaaaaatg cttggtagtc tacttggttt ttctctgcaa attccgccaa cgttcctcga
    23161 ttgtcttcat aattttggct tgcataacta atgctcagat gttttggctg gtatttttct
    23221 cctataacct tgccgttgaa tgcccccaga agataatgga taatggatga ctttcctgca
    23281 ccgttaggac ctgaaatcgc cacccgctga tgaggctcga ttgtaaaatg aattggctcg
    23341 aataacagat tttcataccc taattgaaga tcttctacgc ttaaaagtcg cttatggtga
    23401 gacgcttggc tattcatcgt caaagaatcg atatattcga tatcttttaa tagtttttct
    23461 ttctcgctga tctgggtctc catccgattc acgattgctt tggatcgttt catcgtccgt
    23521 gcagcatcag cacccactgc tcctttattc actcgtctag attcagtatc gatgaatccg
    23581 acttgtttct ttgttttatc tccttctcgg gaacgagacc attcggcttt ttcagctgct
    23641 gttttcttta gcctactgac ttcttttttc aatttttcat tttgagccat ttcaaattca
    23701 tcacgaagtt ttttctgttc ttcatagata gagaaattcc cttgataaag ttccagttga
    23761 cttttttcga ttgctaaaac atggtccact acttcatcga taaatccccg gtcatggctg
    23821 accacgatga agccttgttt tttctttttc aaataagccg ctacttgttt tctaccagag
    23881 atatccaaat gattcgttgg ttcatcgatt aacgggaaat gagtgtcatc cacaaataaa
    23941 agtgccagta ggacttttgt cttctctccc cccgatagtg tgctgaattc tctccataag
    24001 atttcaggat ctgtttgcat caattggagc tctctttcga tttcccatat ctcaaaatcc
    24061 gtaatatcat ttaacacgta ataggttaaa cgttctttat cttttgtctg ttgcgggaaa
    24121 taggcaaatt cttgctgatg gattacttgc ccttgataag gtagtttgtt ttgtagaata
    24181 ttcagtaaag ttgtctttcc tcgaccgttt cgtccgataa gtcctagttt ccattgtgtg
    24241 tcaaaattta gatttgcttg ttcaaataat aatgtgcctt ggctgtcgta gccgaatgtc
    24301 agatttttta tttcgatttt agacattttc catcacctcg taaaaaaaga ccagtcctat
    24361 tttcagaact ggctaaagag gatgcaaggg ttggatacac acaaaaagac ccatacatcg
    24421 aaaaaaccaa gccaatcccg aaaatctgca cactgcttcc ttgtctccac aaggtacagt
    24481 ctgaatgcag ctttcatcaa tcgacttaat ttttcaatgc acggatccct cactttgtcc
    24541 tatatttgaa attacgatag cataaagaaa atgattttgt caactacttc gggaggatgg
    24601 tgaaaaatct gctaagtgtc tgtcctaaga tttttgtttt ttctaatgtt tgcctgtgtt
    24661 ccattgcttc ttctaaacct attggaccca aatgaatact taagacaatt gggagaaaat
    24721 cagaaagtcc ttctagctct tggtctctac ttccgcaaat agcgattatt ggttttcgat
    24781 atttttttgc aagcactgct acaccgtatg gaacttttcc gtggatcgtt tgctgatcga
    24841 tccgcccttc tcctgtaatg atgacatctg cttgttcgat ctttttttcc aatccagtga
    24901 tttggctgac taaatcgaaa ccagaaacca tcttcgctcc caacaaaaaa agggctccac
    24961 caataccgcc tgctgctcca cttcctggaa tatcatctaa atccacctcc cagaaatctt
    25021 ttatttgctg agcaatcttt cctgcttgtt tatctaagaa agctagcgtc gtatctattc
    25081 ctcctttttg tcttccaaat tgccaagcag ctccttgttg acctgtatat ggattggtaa
    25141 catctgcagc gataatcaat tttactttag aaacaagaac gtttcttcga tcaaaatgaa
    25201 gttttttagt agataataaa ggatttcctt ctgtgtaccc ttctaaagaa gcacccaagg
    25261 attggagtaa tcctagacca ccatcgctac acccgcttcc tcctaaagtg acaataattt
    25321 gttctacttc ttgaagcagt gcatctttaa taagttctcc taaggcataa ctggaagcaa
    25381 gtcggatagt ttgatccgtt ggtttatcaa gtagatctaa ccccaatgct tgtgcagatt
    25441 cgatgactgc ggtgtttttt ccttgccaag ttgtcaataa atagctgatt tttcgctgac
    25501 ggaataagag atccatccca tcaatggtac gccatgttcc cctgagcgcg taatagattg
    25561 cttctgcagt attttctccg ccatctgcaa tcggcacatt gatttttctt cctttacgat
    25621 cttcccagcc atttagaact gcctgattta gttcaaatga agaagcactt cctttaaatg
    25681 aatcaattgc cgccaaaata tccatctgta tccctcactt tcttcttaag taagacactt
    25741 tttttcattt ttttcaaaca aaacaatgct attttcttcc atttttctat atactaaaag
    25801 ggaactcatt gaataaagga gagaaaaaac atgaaaatcg caatagctgg ggccggagca
    25861 atgggcagcc gaatcggatt gatgctacat caaagcggaa atgaggtcct cttgatcgat
    25921 cgttggcctg ctcacattga agccattcgt acaaatgggt tgattgctga ctttaacggt
    25981 aaagaagtgg tcgcaaaact gccaatctat tcaccagaag aaatcatcga aagcaacgaa
    26041 cacgtggact tgatcgtagc attaacaaaa gccaatcaat tagatgatat gttttgttcg
    26101 atccaatcca ttattactga caatacttat gttttgtgtt tactaaatgg attaggtcat
    26161 gaagatgtac tggaaaaata tgtgccaaag aaaaatatct tatttgggat cacgatgtgg
    26221 acagctggtc tagctggtcc cggaaaagtc acattgttag gtgatggaga aatcgaacta
    26281 gagaacttgg aaccagaagg cgaagcattt acaaaaaaag ttgtagaagt ctttcaagaa
    26341 gcaaatttaa atccgatcta tagccataac gttcgttatt ccatttggcg aaaagcttgt
    26401 gtaaatggaa cattgaatgg tttatgcacg attttggatt gtaatattgc tgaactaggc
    26461 gcgcaaaaag cggcagagag catggttcga acaatcgtat ctgaatttgc ttctatcgca
    26521 gcaaaagaag gaatcatact agatcaagaa gaagtgtacc aacatattgc ttctacttat
    26581 gatcctgaca acatcgggtt gcattaccca tctatgtacc aagacctgat caaaaaccat
    26641 cgtttgactg aaatcgacta catcaatggt gccatttggc gaaaaggaca aaaatatgat
    26701 attgctacac catattgtgc ctttttgacc caattagtcc atgcaaaaga aggaatcctt
    26761 ggcgcagaat aaaaggctct ctgatgagaa tcagctgaat cagcagctgg ttctcttttt
    26821 ttaagcccaa taaaagatca aaaagaaaac acaactctca tgatgccata agaattgtgc
    26881 ttgtttgttt cagtacataa tctcttcaaa atgtaggttt tttatttgtt ttcaacagag
    26941 gagaggtcat attcgtattg ctcataaata gtggaatcgt tagtgataaa tcgttttgcc
    27001 caattttctg catcataaaa ttccaatagc tctgatagga ttggcttaaa tggtgtatgc
    27061 agaaattccg gtaattcatc taaatcacgt tctcgaagca aaaaaatatt ttcaggacga
    27121 taaaaatgat aatgagcaat cgcttgatta tccgtcacta gtttcttttg aaaatacatg
    27181 gattcaaatg tccgcaaagt cactccttgc tgaccctccc gcaacagttc cagaatcccg
    27241 cgcgattccc ctgttttttt caggtactct tcataagtaa gatatccagg ttgttcattc
    27301 cctcgatgtg gaagaatgaa atagttggtc gtaacatctc tttttataaa ttcctgctta
    27361 tagctttctg ctcgttcttt tctcccatta ttcgttgcac caaaaaatag atccgtcttt
    27421 ggttcgctag aagaaaaatt catatgtttc gaataaaatg aactattatg tttcagccca
    27481 agttcttttg cttccaaaaa atcaaaatga taaatctcat cgattgccgg ttcatcttgt
    27541 gcctgcttca gcatcgcctc gtgttctgct ttgaagtgat tccaaaaaaa taacacaatt
    27601 tttgcattgg taaatgggcg cacgcgtcga gccatctcca tatcaaatcc ataatctaaa
    27661 aaaacgatca gctgataatt tgccaactgt tgtctagctg atttaatgaa ttccgactgt
    27721 agtaaatctt ttccggacca aacatctaaa gagcggatgg aaggcaaaaa ataaagtgat
    27781 ttattttttg caactagcag tacgtcattt ttgttcatct tttccgcctc actttctctg
    27841 tcactttttc ctatgatagc ctgtttaaat agtaaaaagc cagtaaaacg tttcctatat
    27901 ttgtatgaaa atttaacatt tgtatgaaaa tatgatgaaa taaatcattc ttctattaaa
    27961 attcgcctaa aagttttctg aatagttgta attgtctgaa aatttgttat cattagtcat
    28021 tgttaaatat tgctatttat tcttaatata aattttgatt gtgaatttaa aagaaagagg
    28081 tcatttatgg aagataaaaa attgaaaaaa caacttagct ccagacatat caccatgtta
    28141 gctttaggtg gagcaatcgg tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc
    28201 gccggccctt ctgtattgat tgcttttttg attggtgggc tgattttata tatcgtgatg
    28261 aaaggtctgg gaaaaatcgt tttaagcggt ggcgacacgc atcacggatt atctggtttg
    28321 atacgccctt atctaggtgc tcattctgct gattttactg attgggtcta ctggtctatg
    28381 tggattatca atatcatcgc tgaagctgtt gcagctgctt cctttttgca gttgtggttc
    28441 ccgcacattc cagcctgggt atttgttttt atgttggctg ttttaactac gatcatcaat
    28501 ctttattcgg tacggttatt cgcagaaaca gaatattggc tggcttttgc taaaatcagt
    28561 gtcattatct tactgatcat ttttgctact tatcttgtcg gtcagcaaat gttgggcaca
    28621 ggcgttttcc ctacccttca gcaattgact gaccatggtg ggtttactcc tcatggtatg
    28681 aagggaatca tcagttcttt gcttgtagtt atctattctt acggcggttc tgaactgatt
    28741 gctatcacgg taagcgaagc agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt
    28801 gtcatgggaa gaatcatttc cttttacatc attcctttgt ttttgctttt gattcttttt
    28861 ccttggaata ctttagcagg cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg
    28921 aatatcccat ttgcagcgga tatcgtcaat tttgtgattg tccttgcttt gttctcatct
    28981 atcaattcgg gtgtgtatgc atcttcaaga attttgttct tccgtttgaa ggatcgaaaa
    29041 ggttcctcta gaaaactagc tgttttaaac aagcatcaag taccgcaacg tgctgtttta
    29101 ttttgtacca gtgttcttta tcttggtgta gctctttctt attttgtcgg tgacaaatta
    29161 tttggctatc tggcaggttc cctttcgtat acagtattat tgatctggat cattatcagt
    29221 gcggcatcct ttgtactggc actaagaaaa ggaacattat gggataaagg aatcagcttt
    29281 tttgcattag ctgtcttagg attgattttc cttggtatcc tgttcacgaa ctccattggg
    29341 gtaactgtat tgactggcct gctttacctg ttcatttatt tcagttatca aaaaaagaat
    29401 gatgcgttcg tattgacgaa tgaatgaaat cgatagaaaa ttttatttta tttatgtgga
    29461 atagatggag ctattgaaga aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc
    29521 gaataaacag tctcatgacc tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa
    29581 ttgattgttc tccatgctca tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt
    29641 ttaaatgaaa aagtattgga agtttttgtt gatgatcggt gtttcaacgg tagttatgtt
    29701 tggtatgatg tacttgaatg tctatgcgct cgatcatatc ttcttcagtc gtacacgagt
    29761 attcatggcg ttgatgatgg gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa
    29821 aatgtatgaa aacaaaaaga taaatgttat gatcttagcc gctagtcttc ttgtttttgc
    29881 cggttccttg tttatggtac gaagccaaac tactgtcaac gacacagctt ggatgaaggc
    29941 catgattcct catcattcta tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc
    30001 tgaagtaaaa aaattagcgg aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat
    30061 gaaaaaaatg atcaaagaaa tggaatgacc attatccgtc caaataaaaa aagaggatgt
    30121 tccagaagtt atcagcttca agaaataaga agaagctact tctggaacgc cgtgaataag
    30181 gggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagcttct
    30241 cctatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
    30301 aagaggttgt aaaatcagcg ttctgacctg agtattagag agacaaatgg aaaaatagtt
    30361 cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa caccgtttat
    30421 cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct
    30481 ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    30541 ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc
    30601 taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca
    30661 acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg
    30721 caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca
    30781 aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg
    30841 atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag
    30901 gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa
    30961 atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa
    31021 ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat
    31081 actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac
    31141 aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa
    31201 aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt
    31261 ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt
    31321 tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg
    31381 tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat
    31441 atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc
    31501 ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag
    31561 cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat
    31621 aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg
    31681 gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc
    31741 tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat
    31801 tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat
    31861 cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt
    31921 taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc
    31981 taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat
    32041 gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt
    32101 ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat
    32161 taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta
    32221 attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta
    32281 accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca
    32341 atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa
    32401 gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg
    32461 ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc
    32521 gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt
    32581 gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt
    32641 tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt
    32701 ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa
    32761 agaaggagta acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt
    32821 gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa
    32881 tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt
    32941 ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa
    33001 gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt
    33061 ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt
    33121 cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc
    33181 gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc
    33241 ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt
    33301 cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc
    33361 aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga
    33421 aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct
    33481 ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc
    33541 aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag
    33601 atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca
    33661 tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt
    33721 ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt
    33781 attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc
    33841 cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc
    33901 gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa
    33961 ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc
    34021 ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc
    34081 atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt
    34141 caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc
    34201 agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc
    34261 aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc
    34321 caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc
    34381 tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg
    34441 gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt
    34501 tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa
    34561 attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt
    34621 ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc
    34681 cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac
    34741 caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg
    34801 cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag
    34861 caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg
    34921 ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg
    34981 aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc
    35041 cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga
    35101 atgctgtcca aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa
    35161 tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg
    35221 atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat
    35281 ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc
    35341 aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt
    35401 acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa
    35461 cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc
    35521 gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct
    35581 tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata
    35641 accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt
    35701 gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc
    35761 ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct
    35821 cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct
    35881 ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc
    35941 gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt
    36001 agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga
    36061 gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc
    36121 aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa
    36181 acatcgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt
    36241 tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa
    36301 aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc
    36361 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca
    36421 agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc
    36481 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
    36541 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac
    36601 ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct
    36661 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt
    36721 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
    36781 gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac
    36841 gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg
    36901 aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac
    36961 agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata
    37021 atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc
    37081 tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg
    37141 ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa
    37201 tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt
    37261 cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat
    37321 ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc
    37381 ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat
    37441 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg
    37501 gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac
    37561 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
    37621 aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc
    37681 gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc
    37741 agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag
    37801 cctaattcag caccaaacct tgtaaaagga aggacagaac cgacaataat tcctaatgta
    37861 gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc
    37921 agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat
    37981 aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca
    38041 gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt
    38101 ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc
    38161 aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga
    38221 ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc
    38281 agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata
    38341 ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct
    38401 aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg
    38461 atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta
    38521 tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg
    38581 aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc
    38641 tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact
    38701 aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac
    38761 gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg
    38821 tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca
    38881 ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt
    38941 cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc
    39001 atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca
    39061 acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc
    39121 ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc
    39181 agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt
    39241 ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta
    39301 tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact
    39361 gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga
    39421 agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa
    39481 tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg
    39541 atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca
    39601 gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct
    39661 tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg
    39721 ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc
    39781 aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc
    39841 atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct
    39901 gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca
    39961 gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata
    40021 gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc
    40081 gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct
    40141 ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa
    40201 ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt
    40261 tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc
    40321 tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg
    40381 tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat
    40441 aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct
    40501 catctccttt actaagtaat tttaattcat gacttccaat catttttcga atagttattt
    40561 ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa
    40621 cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat
    40681 ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct
    40741 tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg
    40801 ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg
    40861 ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt
    40921 ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc
    40981 ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt
    41041 atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca
    41101 aagggactgg ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa
    41161 cattatcagc acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc
    41221 ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg
    41281 gttggagcaa caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg
    41341 ccggggactg aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc
    41401 gcccgctttt tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac
    41461 aaattctaaa ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa
    41521 ggacctccgt tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc
    41581 tttcgtcttt tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa
    41641 tgtttcatag atcactgtac ttctttcttt atgtgctaag ctccgtttca tatcttcatt
    41701 cacactacgt gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa
    41761 aaaagtaaag agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt
    41821 gattttcaaa taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag
    41881 aatagatagt tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg
    41941 gcattcataa cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg
    42001 aagattcaaa accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct
    42061 gatctcttta taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt
    42121 ctttaacttt attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat
    42181 gttcattcgc tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt
    42241 ctcaatcccg actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa
    42301 cttcttgttg actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt
    42361 taatttaaat ttttccacat tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa
    42421 aaataaaaaa atgttttttt cataaaaaat accaaccttc aagcattaga atgttgtcta
    42481 actactgaag gttggtttga ttgtttattg attatttttt tgctgttcaa cgtctgttct
    42541 tgttactgga tataagttgg ttgttgtttt attaccattt tttgaaaaga cacttgtcga
    42601 atgttcgcct ttttgctttt cgattgcttc attacgttcc gcttcatttt tatagtcata
    42661 ttttttcgga tcaatcgtct caaatccgtt tggcacataa aaccgcaaga gattttcctg
    42721 attcagctta tctgaaagac ttaacgcagt cttgactttt tcctgatctt tggagatttc
    42781 ctcatcgatt cctgctgctt tcgtatcgat gatttctcct gtctgattat tgtaagcttt
    42841 tcctcctagg acggtgtatt tgggtgtcac aaagttccca tttcgaaaag caactacttg
    42901 atcatgctga gaagataata agtctgtacc aaaatgaaga taatctttat catcgattcc
    42961 caatagatgt agtaatgtcg gcaaaacatc gatttctcca ccgtattcat gattgactgt
    43021 ccctttagta taaccaggca tatggatcat caatggcact cgttgcatct gtgcgttgtc
    43081 aaattcatcc catgtatctg gatctttacc aagagcgctt gctaaatctt tattatctgt
    43141 attcgagatc ccaaaatgat ccccgtaaat cacaaagatt gaattttggt aaataccaga
    43201 ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc agataatgtg ccgttcgaac
    43261 ataattatct actgtgctat tgccagtatt caaagatgga aagtcgaaat tcttgtcatc
    43321 cgtataataa ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg gctgttgtag
    43381 atgctcaaga tattgggcag attctctgaa taaatcttta tctaatatcc cataacctaa
    43441 cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa ttgtcataac ctaagttttt
    43501 atatacatga tctctattcc aaaaactagc tacattccca tggaaaacag cgcttgtata
    43561 gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag acattgtctg acccaagttc
    43621 tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc
    43681 acttgtcttt ccctgtccta cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt
    43741 ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg tctaccttca tattaatcaa
    43801 aaactgctgg aaactttcta aatggagaac gatcacattt ttccctttag cgatcccaaa
    43861 tgtttcggga tttggctctg cataatgttt ctttgtataa tctaaaactt catcaatccc
    43921 attactgctt gcatgcgcgc gaacactgct agtcattcct gttttgattc cgtcataaat
    43981 agtatacgca tcgattccta gatatttgac aatatatgaa cggtcaaatg ttcgctgcag
    44041 caattgtgga cggttggctt cgctcagcgc tagatttcct gaaaaaataa gccctgaaag
    44101 acaggtaaca gcgatagcca tcggtttgcc gacagggttg cttttgatag gtattttctt
    44161 gaccttcaac caaaccagta gcccaatgaa cacagcgata tccagccagt agatgacatc
    44221 atggggcttc atcaaagaaa aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc
    44281 taatacagat ttgactgtaa tgaaatctgt gaattcccga taaaaaataa tattcaagta
    44341 caacaaaagc gtatttaaaa tatcaatgat caaaagaata gctaacgctg gctttggctt
    44401 tttaatatac aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt tgatccacaa
    44461 gataaagtac tggatcgtac cttccacacc taatgaaaaa tctgcatagt aggcaatgat
    44521 cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt gctaaacgat tttggcacaa
    44581 ttttttaagt tgtgtcacgt atatcacttc tctctattca cattttttct tattttgctc
    44641 ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag atttttttat tctttctaaa
    44701 gattattccg tccacttttc acagcataaa accgctgcga tgtaagcgat taaactaatg
    44761 aagagataaa agtcttgtgg tgaccgcttc actccacggt attcaaaagc cagtttctcg
    44821 gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt atttcttgat
    44881 gcaggacagc tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc
    44941 acaagtcaca atatttataa aacatgagaa gctgtttttt gaaattgttc cttgtggctc
    45001 agagttaaac ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccctcgg
    45061 tattagatta tgtcaggacg ctaattttac aacctcttgg taaacggtgt tcaaaagctt
    45121 gcatctgcgg taataagccc aaccaaacga aaaatataag gagctatttt ttgaaattgc
    45181 tccttgtggc tcagagttaa acggcaggct caagacctct tgatacacgg tgttcaatca
    45241 gctgaccttc ggtattagct caaaaaacgg caaaatatga ggaactattt tgccgtttgt
    45301 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tgtttttcat tgtccatagg
    45361 attgatattg tccttgtgat gactcattcg taataattga ggcttgattt tcttcacttc
    45421 ccgttatcgt ttgtccactg ccagaatctg aaggacttgt tcctgtcgac tcgttttttt
    45481 gagaagagac agttgttttc ttgacttgtt catttgtctt tttccattcc gctaaacgat
    45541 cggcgaccac ttttttgtct actttttcag aataagcggt gatcactttt ccatttttca
    45601 aacgggctac tttgtcgcca tcatgagtca aaagcaaaac ggtcaaatgc ttgtctttaa
    45661 attgatattg tctgagagaa atcgcactaa gtacgtcatc gatattaata aattgatttt
    45721 cttcttttcc ttttggcaaa taaatgtcca attcgccttt ttcgtaattg gcagttattt
    45781 tatactccgc ccaccaataa gttgagaatt cttttttgaa attatctact gattctttgc
    45841 ttgctacgat tttttctgtt tctttctgag caagatctgc tgtcattttt cctgaaaatg
    45901 ctgcggataa aataagcagt aagaaaaaac cgaataacca aaagagaaga aacggtcttt
    45961 ttttcctcat gctttcctcc actcctattt aattttgact attctattat atacaaaaaa
    46021 tgaaatagaa agacaaaaaa gttgaaaaag atcgtaaata attgaacttc tttttcacaa
    46081 aatggtaaga atggagaatt tcacgcattt gctctcaact attctttctt aagcctcaaa
    46141 tccgttttta tctgatacgt ctttgaacca atgacccgat tttttgattg ttctttttcc
    46201 atcttgtgct aagtctactg aaataaagcc atatcgattc ttatatgcat tggtccacga
    46261 ccagttatcc atacatgtcc acatatgata gcctaaacaa ttggcacctt cttgaatggc
    46321 ttgatggaca tatttcagat gagattggac aaattcgatc cgataatcat cgtggatcat
    46381 gccatcttct ttgataaatc gtgcttcgtt ttctacaccc ataccgtttt cagaaataaa
    46441 gcagtcgata tttccataat gatctcgtac attgatcaaa atatcataga ttccttgttc
    46501 atagatctcc catccgcgat aaggattcat tttacgacca ggcatttcat aattgtcgaa
    46561 ataatcctca ggcattggac cgtttttagt atcgattggt gtttcttttg cttttacccg
    46621 gcgtggctgg taataattga tacctaaaag atctactgta ttttctttga taaccgtcaa
    46681 atcctctgca tggatcacag gcatgagatc aagttctttt actatctcaa tcaattcact
    46741 tggaaactcg ccttttacag ctggatcaag gaatgaacga ttgaagaacg catctgctat
    46801 ttgcgctgct ttaacatctt cagggtttga ttcgtctctt ggatagcttg gtgtcagatt
    46861 caagatgata ccaataacac caccttgctc catttcatgg taagatttga tggctaacgc
    46921 tgaagccaac gcttcatgat agccaacttg gatcgctttt ttcatatctg actcttctgg
    46981 ataatgccat cgatataaat aacctgcttc gactggaacg attggttcat tatgagtaaa
    47041 ccattttttt actttcgaac caaacaattc aaaacaactt ttggcatatt tttcataggc
    47101 ttcaaccgtt tctcggttca accatccgcc tttttcttgc agatacatcg gcatatcgaa
    47161 atgatacaga ttcacaaagg gttcgatacc gttttcaatc aactcatcaa aataacgatg
    47221 atagaaacga acggcttctt cgttgattgt tccagattca tcaggtatca aacggctcca
    47281 ttggatagat gttcgaaatg aattatgccc agtctgtttc atcaactgta catcctcttg
    47341 atacttcgta taaacttgcg aggttttttc aggtcctact tgatcaaaga acttttgcgg
    47401 ttcttttttg taccaataat caaaaatgtt ctctcctttt ccatcgcctg aaatccttcc
    47461 ttctgtttgt gggccacttg ctgcagaacc ccaccaaaaa tttgctggaa attgatattt
    47521 tttcatcaaa gcacctcttt ttctttttta gttcgtttta gttgattcca tgtatgatat
    47581 tttccatcac aatcactttt tcattttctt cctcttcttt tttgattcgt tttaacagct
    47641 gttctgctgc tcgttccccg attttatctg ttggtggcgt gatcgtgatt ttcggaagga
    47701 tactcagatc gtttttatct aaataatcat agtgactgaa cgcaatatcc ttcccgattc
    47761 tgacaccttc ttgattgaaa cattcgatgg caccaatcga catattgtaa ttgctgacaa
    47821 aaatagcagt tgcccctttt tctaataatg cttttgcccc gtaatagccg ctttttctag
    47881 aatagtctcc tgtaaagacc tctaattgga tcgatggatc attttggacc gcttctttgg
    47941 ctcctgctaa acgttctctt gctacgtagt ctgtttgagt ggcagcaata aatccgattt
    48001 ttttatgtcc ttgttcacca tgatgagcga tcacttttgc tacactttct cgatcattca
    48061 ctaatattgt atcaatacct gctggtcctt ctggacaatt caatgcgata actggaatac
    48121 tcatttttgt gagcatttca aatccagacc actcttcttc ggataagaag acaatcagcc
    48181 catcgatcac atgcttcatc aggtattcaa ttttttgttc aattttttct ggctgatcgg
    48241 caaacccgct taataataag ctatacccat tttcttcagc tactctttcg atccctgaga
    48301 caacttccga cccaaaacgg ctggaaatcc cgtttatcaa aagtccaata gagaagcttc
    48361 gattattctt caacccttta gcaatgatgt tttctttata gtccaacgct gaaatcgctt
    48421 ctgctatttt tttcatgttc ttttccttca gttgctgacc attcaagtaa cgggaaactg
    48481 ttcccactga aacaccagcc atcttagcca catcacggat cgatgccata cattctcact
    48541 cctgccagtt acatttgatt tctctttctt gttgttcgat tatgatgacc gcattctttg
    48601 catagtgttg cttgtcatca atatccatat aactaaagtc ttcagtagag caacttcctc
    48661 tcactattaa ttatacttaa acaagtattt gaaccggttc atttttaata tagcattttt
    48721 ttaaaagatt gcaacacttt tttaaaacgc tttctgaaag tggcggattt tttgcctttt
    48781 taaaatttta ttctaaaatt tactctattt gtgggtgttt tgtgcgtttt tctcttccat
    48841 ccgtttgaag ttttacattc caatggtata atttctttat cctatcaaat gggggaagta
    48901 ttttgaagaa attaatcgta ctatttattt tgtctgcggg acttttgaca ttgactggct
    48961 gtgatttgtt tggcggctct gaccaatcct cggacgaaac aaccgattct tctgtcttaa
    49021 aagcggaaaa agcagatacc accaacagtg tatatacgct aaaagtacgt aaagatatgg
    49081 atttatccgc gggacagttg acatttacag ataaaaaaat ggaatggaca cgcacgtatc
    49141 agacacaagg aactgattct tctgaatcat ctgaagaaaa aacagtgctg acagatatca
    49201 agatcactac caaagatgac gaatatatta tttcaggaaa agaaaataaa aaagaaacaa
    49261 cagtcatatt tacaaaaatc ggtaattacc gtttgaagga tgaggatggc aacatctaca
    49321 gtctatagtg ccgtctgttg gatcttcttt tgtttctccc cttcttttct gctatagtaa
    49381 agaagcaaga tgattgtaaa ggagacgaaa tggatgatcg atagtcctga ttattgtaca
    49441 tgtaataaca acaaaaatat ccatacagaa tttaatgacc aaggaagctt gagcatatgc
    49501 gacaactgtc acaaactagt caaagattca tttaaaacat ttgaatctta tcctgttcaa
    49561 aattatgtaa aaagtagaag aagataaaca aatccaagca aaactcacta tggctgattt
    49621 ttatgaactt caaactcagc ttcttccttt gatcaagctt catcagtaaa acaaacagtg
    49681 gatcatttca gtttacaaat caaagagtaa agatttattt atttcaaata tttgaatata
    49741 aaataaccaa acgatagaaa ggcttattct acatcacgta gtaatttttc tttcccgtcg
    49801 tttggttttt tatttttagc ctaattcttg accttttgca tgctggttat ccgtaaaata
    49861 ttttccagca ggctttcctt ccaattcagc taatttttct tggatctgac ggatcacctt
    49921 ttttgcatct tctggatttt ctacaaaatc gaatttatcc ccatccactt gtattttagg
    49981 acatacatca aaatcttcat accattgatt atagcgggtg ttcaacgttt cataataggt
    50041 atataacgtc tcatcgcttt ctagctgttc atagtcacgc ccacgttttt tgatacgttc
    50101 tagcatcgtg tccaaagaaa ctttgatatg gaccatcaaa tcaggccgtt tttttggagc
    50161 aacatcatct aactcgttta gcatcgtatc taataagcta tcgtactgct gtacttcgat
    50221 atccgttacc cgacctaaat cagcgtttag atggaacaac aggctatctt catagatgga
    50281 tcggtctagc acattgtcat cgttcactaa agcatttttc attgctaaaa atcgcttgtt
    50341 caaaaagaag atttgcaata aaaaagcgta ctgctcagga tttgaataaa agagcggcaa
    50401 tacttcgtta tcttcgacat tttcataaaa tggtttagaa tcaatctctt ttgcaagcat
    50461 atccgtcaaa ctgctcttac ctgcaccaat cgttcctgaa atcaaaatca ctgacataca
    50521 acccttcctc ttctataagt actcgcctac gtaagtttca acaggcaatg tttcaaaatc
    50581 atattcacgt acagcttggt ctgattttaa atacgtatgg agactttgtg taatcatcaa
    50641 gttcgtaggt accgtctctt cttgtacaat gatcatcggt tttttcaaca tagttgccac
    50701 acctaattcg taagccgttc ctggatcgat tgtttgatgc tcaaagtcaa ggatagcaac
    50761 aacaaattct gcatttgtca gttcttccat atctttttcg tatacttcct gcgcccatcc
    50821 agcgctgaat agttcataat tgctttcttg gtgatgacgt ggagagtaga aacttgtgac
    50881 tgttttgttt tcctccaacg ctttttcgat acgacttact cgatcgattt gctcctctga
    50941 aaaaaatggt cctgctaaat atatttgact cattattttg cctccttcaa taataactga
    51001 tttttaatag atactgctcg taattcattg accagttcca atggattgtt ggaaaagatt
    51061 acttgatcca accatttttt atccataaaa tcctcttttg caaaagtttc taataattgt
    51121 tgcaagccgc tgtaataacc gccaatattc aatattccga ttggcttgtc gggcgtcaga
    51181 ccgattgcta tccaagaaag aagttgtgag aattcctcca atgtacctag acctccaggg
    51241 aaaacgagga cagcatctcc cttttcaaat aataatacct ttctttcatc gatcgtttct
    51301 gtaggaataa atgtagtaac atcttttcca ggcaattcag tttcaaaaag cccattcgga
    51361 tagatacctg tgacttttcc gttattttcc agtactgttt tactaatcac tcccataatc
    51421 ccggagtatg aaccaccgta aaccagttcg atttcttctt gtgccatgta ttttcctaac
    51481 gtttgcgcaa taaatttgta actttcttta ttaccaaaac gggaaccgca aaaaacagta
    51541 agtttcataa aaaaacactc cctaactcaa aagtctatct tcattctaca cgtgtttttt
    51601 taaaataaaa gactagattt tgtagttttt tagtggtata t
//
LOCUS       DAHHPA010000008        49821 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.008, whole genome shotgun sequence.
ACCESSION   DAHHPA010000008 DAHHPA010000000
VERSION     DAHHPA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.008"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(980..1153)
                     /locus_tag="NBH55_000421"
     CDS             complement(980..1153)
                     /locus_tag="NBH55_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508437.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHLFIFAYILAMIA
                     FLSGGWLIIYLLS"
     gene            complement(1156..1554)
                     /locus_tag="NBH55_000422"
     CDS             complement(1156..1554)
                     /locus_tag="NBH55_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508438.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKELYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1770..2825
                     /locus_tag="NBH55_000423"
     CDS             1770..2825
                     /locus_tag="NBH55_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HCD1508439.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVIEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2845..3198
                     /locus_tag="NBH55_000424"
     CDS             2845..3198
                     /locus_tag="NBH55_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508440.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3380..3676)
                     /locus_tag="NBH55_000425"
     CDS             complement(3380..3676)
                     /locus_tag="NBH55_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508441.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3680..4498)
                     /locus_tag="NBH55_000426"
     CDS             complement(3680..4498)
                     /locus_tag="NBH55_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1508442.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4724..4945
                     /locus_tag="NBH55_000427"
     CDS             4724..4945
                     /locus_tag="NBH55_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCD1508443.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4947..5213
                     /locus_tag="NBH55_000428"
     CDS             4947..5213
                     /locus_tag="NBH55_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCD1508444.1"
                     /translation="MSYQVRYEREAQKTLKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5380..5865
                     /locus_tag="NBH55_000429"
     CDS             5380..5865
                     /locus_tag="NBH55_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508445.1"
                     /translation="MGWYGAYSSANEVIEEKKGYAKNGLEIVDTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKRQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            6356..6640
                     /locus_tag="NBH55_000430"
     CDS             6356..6640
                     /locus_tag="NBH55_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508446.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6647..6892
                     /locus_tag="NBH55_000431"
     CDS             6647..6892
                     /locus_tag="NBH55_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508447.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6914..7168
                     /locus_tag="NBH55_000432"
     CDS             6914..7168
                     /locus_tag="NBH55_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508448.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALAYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7250..7435
                     /locus_tag="NBH55_000433"
     CDS             7250..7435
                     /locus_tag="NBH55_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCD1508449.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7502..7897
                     /locus_tag="NBH55_000434"
     CDS             7502..7897
                     /locus_tag="NBH55_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCD1508450.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7887..8264
                     /locus_tag="NBH55_000435"
     CDS             7887..8264
                     /locus_tag="NBH55_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508451.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8286..8660
                     /locus_tag="NBH55_000436"
     CDS             8286..8660
                     /locus_tag="NBH55_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508452.1"
                     /translation="MKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFYKSDCYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8966..9364)
                     /locus_tag="NBH55_000437"
     CDS             complement(8966..9364)
                     /locus_tag="NBH55_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCD1508453.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9560..10321)
                     /locus_tag="NBH55_000438"
     CDS             complement(9560..10321)
                     /locus_tag="NBH55_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCD1508454.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPNKLKKT
                     GEIVLPVKAIIKYMDVDQNLETELKNIVNTLQRQAVYEISNLDKEYKTYATVSAIDQI
                     HWDLEEQIITFNFSQEVIPLILSIKKKYPYFVFQDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKN"
     gene            complement(10357..11580)
                     /locus_tag="NBH55_000439"
     CDS             complement(10357..11580)
                     /locus_tag="NBH55_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCD1508455.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKMFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTIKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIAELRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TDIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKFMSRLQNKVYPLYDRLSKDRGFESVVSHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            12544..13347
                     /locus_tag="NBH55_000440"
     CDS             12544..13347
                     /locus_tag="NBH55_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCD1508456.1"
                     /translation="MSQEVTENVIDNDVDLVSDQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNVFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQQETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFESFDYEKIYAKKGRKVDRLRFTFSEPESNLPSISMHNWLEED"
     gene            13826..14269
                     /locus_tag="NBH55_000441"
     CDS             13826..14269
                     /locus_tag="NBH55_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508457.1"
                     /translation="MDAINWHEFVNYMEKLLDDLKSFSIADAVKHGLPVSTTMKMRGL
                     KVPDEIIKLRTKTIIETAKIVDSMNLDLKGKKTTYEKDFVRFLKNVIGKYLGKKSFTE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIVEYLENKEQKKGR"
     gene            14388..15446
                     /locus_tag="NBH55_000442"
     CDS             14388..15446
                     /locus_tag="NBH55_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HCD1508458.1"
                     /translation="MEKDVNSASTKDQYNIIGGLLGDKDKRTRHGLRIMLATFLGVFL
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLTFARSGAEITISDVWTDKTRDVTVVKLG
                     YSGNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDYTADDDTKVNVNESDSVTKALSQYSMNDVDNEGVISFGKKAKPVN
                     TPDNINFRINPYSDTTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKTINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15488..17437
                     /locus_tag="NBH55_000443"
     CDS             15488..17437
                     /locus_tag="NBH55_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCD1508459.1"
                     /translation="MENSTEFFKNIQTEFNHALTLMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAKRQRQEGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TIIPRLKEYERILKSEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPEASLK
                     LSKKIPDELLEEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDIAKLEEELKSIKGQPVAVYTVTMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRKLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVNDTVSLSNTILFATTNAGAE
                     MYDSMNRYSSLSRVANQENSNEYSTELKREYRKVALEIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAHNEILKSAELVRKHLNVDLRLPPQKKHFYGQEFYADDLSMHLV
                     FDRASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSKAGGFEGKGDT
                     DAEIIAKPSIAESKENKFVFSVGGV"
     gene            17440..21129
                     /locus_tag="NBH55_000444"
     CDS             17440..21129
                     /locus_tag="NBH55_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508460.1"
                     /translation="MKFKKSLLQNRKIKRWVVRGLVIVGALAVTLGAPFGMNDPTSDD
                     QGNKQETVANTNVAYAESKPAEKGVWTLYARILMGDKEVNDSATKTGQMNLLGQGGIS
                     ADFPYSDISKQGNAIAGGNAGTQLASTLATYNYYGYIETVSGNAIASQASSFIGGIGR
                     TLGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGKGGSDVSNPVAKALTSLL
                     NNLGLNNQLFKAIASLSMLFFTAILGYQGMKLLSKEGVNSKGLSPVLEKWIVRIFVLF
                     VFLPVAGSLLGVYINGIQSAKEDGKLPTNVVGDYIINTRLWASTQNLAPSSDTGGNVP
                     NGKPTSEIKFVDTDYEPSKKSEMIRMINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NVNTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGNAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTTNNFDATQPWGVANNATFSTQSVALMLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFVRVWEVVLTGSLAAGAGVMAISLGVVTTSLIGLQLPIFLITFVRAFGDTAV
                     GFIPNTLLGDGVANIVRSAMVLGVGYLLVFKKGEKVNNNLINMLIGLPLEVAFGFDER
                     AKLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGRGSVKDMVAGGLGAVGAQGIAD
                     RLNNMGDSNADAKTIADKVVPETPASVPFNGGGVSGTINNTSFKDLVRQDQLRNNSQQ
                     YSDHLGRMVDEKGNFVDENGEVLKDSKFGKDAKGNFIDDKGRLVDEDGNLIDTNGKPL
                     EDNEDGNIGMDSQGRLIDDKGRLVDDEGNLVDENGRKLLNGDFGQDVNGRLVDDKGRL
                     VDEDGNLVDEFGKKLGKHSNGYNVNGDTVDEDGNIIDSRDALVADRNKELEQQQHTPI
                     AVAIPDDESDEVILDQAHQGLREEAKDKVVSDQAQEELKNKSNGEDVSNQYHQTAGNV
                     PVEGQSNELHNNFDNVADRNDTVLKEKESLRQETDKDQSKLKKTTIPTPIGSTVPKVN
                     QANDSETNTGLGMAKDNQSIREPKKTTESSKSNKSKSTSNKTGKSKEPSKAKSAVEYL
                     SKTGKNVYTSADKALAKAPKGSLAGMTYEAKNEAIKLGKGTAKVAKVTAKGATKTGKA
                     VKKSTMSVKDYIAKYSEQKPTDKKS"
     gene            21541..21879
                     /locus_tag="NBH55_000445"
     CDS             21541..21879
                     /locus_tag="NBH55_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508461.1"
                     /translation="MFDSIKNLVSGMTPEMKTLVQAILYLMATFFALKPGFTAMKHFG
                     DAKWGEGLKYVGAVVAIFIIPVITTVMLLTMGTKTGNDLNQKANMISALIPVVGIYLV
                     TKFPKEEQLG"
     gene            21991..22227
                     /locus_tag="NBH55_000446"
     CDS             21991..22227
                     /locus_tag="NBH55_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508462.1"
                     /translation="MDREKMLVHANEVLTSGFKGTDLAKIMNMNVNQFYDYRNGNKKI
                     EKARLETLIKFEKAYLYVLDKQKRTIDRKKGVLQ"
     gene            22227..24392
                     /locus_tag="NBH55_000447"
     CDS             22227..24392
                     /locus_tag="NBH55_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508463.1"
                     /translation="MAKFKDITESINYVAETVPVVVLSLKPQLLEVYQKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYVVIEDVQKLSSEEFPDEEDAILDSEKYVVLLKSSLN
                     YVVDLPEKFKEKLLAGVRFMDSNGQLLVIPDDPNMFEREVEEATDFYSAVDQLEVATK
                     GLERIAETFNSSDAVEFEAVAEAETEAVPDEEEALFEDNDEATEAGNQEASIEETQTE
                     TYQQPKIDEKPESQSDKENYVYEAEKIVSESDVPKNLDADLLEKDFEQIIANKFQPVV
                     LDEVSDPVDLNEEMRSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLLLKGK
                     QMLADELLRIEEATSLSGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLEAELKKLEQRYDAEHLPEVKQQEDEFRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIEKSLTEVQEKTLEIVEKTKAENEAEFHDTKEKTTDVVVK
                     YFANKEEVEKLVAKDTNELESKLVAQQNIIEDYKNRVKEFKDLADGYKLNSQKLTEQS
                     EALREDLSRARNDLSQMAEKTKSLVVTTENQTESKRDFTSKKMATIIAVSAMACGTIL
                     GSSYFISHNHQEAQAQTETSISRVENSLGSNTASTSTTDNNYEATPTTEFKVGEDVVI
                     SIDGKTTKAEVLSIDGDKAVVKAPDGNQYKIQAHLTEKN"
     gene            24404..24634
                     /locus_tag="NBH55_000448"
     CDS             24404..24634
                     /locus_tag="NBH55_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1508464.1"
                     /translation="MREENIVYPVIENQANTKSSQFLQEVQVVYPVNELPQTNETVSK
                     SISLIGLLIVATALTVGVYRKLKVKGSISDGR"
     gene            24624..25490
                     /locus_tag="NBH55_000449"
     CDS             24624..25490
                     /locus_tag="NBH55_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508465.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVDWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLRFIFIPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNTARVTMGKKKPFRGTEILKVRQDGTIMYRDGSVALMFNLDGATSLTAFP
                     EEIIAQEGKSIQYQNARDRSLAEIKITSSQPQNTERQIRNMKVQKRNAKSNKAVFDIV
                     DQQERFVSEYIDGVKTTVIQYLILKSQSQMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSEIYALK"
     gene            25508..27466
                     /locus_tag="NBH55_000450"
     CDS             25508..27466
                     /locus_tag="NBH55_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508466.1"
                     /translation="MELMSRSKLLNFRKKKHKKEQNADSSKAVNNTYVSDLTSLVGYQ
                     FTPSYIRTGNRWGTIVKLVNTYGMNRNYEFGWMVKAIPEVKVEGVKAYLIEADKPVSQ
                     KDQQEIMHENVGGSTNSYENNLNDKNSDAEINKTVMRIIDLNTASLLDSTANKIIDWK
                     LEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSFGGDQRDLFDRLLSEPVGSVYEY
                     TSMSSDFAGNDHAIRKGLDDADGWAIGTMSTSYASGVAFMAMDRSFKGKVLVASHKDA
                     TIKGYREEISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGETFVAKPSLNSV
                     IEKINLAEEGLNSLELFGDRKDIGDLYRRNLDKLTQIFYLLADRELNPATRVLLKKEL
                     GNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKTGTEKEIDRII
                     VLKDTLRTALDSFSKMFNQRTTLKEIGKSDKLQFYYNLSDLRTEINVMEAQFLNTFDF
                     ITSQAKKGDIVMIHGFDRLSVETVGLIKKRLTMLEEDGVRLAFLFDTIGSGEDKDGIE
                     TADIFNTDGKIYQSFESDFNYTIIGKMTSEELMKYEKKVAPARKGEDDQKLSVDLRQY
                     LTSGRSNKFQIRRPVDLTNNFVIGEFII"
     gene            27605..27940
                     /locus_tag="NBH55_000451"
     CDS             27605..27940
                     /locus_tag="NBH55_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508467.1"
                     /translation="MKQYLNEWKVIEGSLVAQRIKGLPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDEKYFIIQEYEGQLTPFYTTWEMSEDGINNFPCESI
                     EESISQTEC"
     gene            complement(28466..30061)
                     /locus_tag="NBH55_000452"
     CDS             complement(28466..30061)
                     /locus_tag="NBH55_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508468.1"
                     /translation="MFILDDEINKVKNPRIVPLLEEVRSSFYQGNYRSAIAVNYSALV
                     LDLIDKLSDLANIYEDDTSKKILEEIAKLRKSDPNSPRWEMELLEKTYNSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGDTWELREITKETCQDVIRKSYEIVFLKNPILGKKV
                     TNEIIQYASRIHSMSVTPEEFHSALKNSYLKKLSEKAKENLCKTAFKFVFKLPYGNEE
                     VDRDRVSTFKLLDALIFDNKEFYLKILKKEISNYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIASHPELQRDFSEEIIQNLKISIKDFEPKQPITTHIEDYYRLRSLAVLGNT
                     KEELQEHLNRLLDPILQQNMRVSSLATSTLETIYNQYLYFGEKEMFLDFMIEHLTDSP
                     AFRYSDKDMEILPWIYDKLNQDQFNKLLYNLNRNNQFYYNGYFSSFISNLLDFYKQKF
                     HMDLRTHYNGLLYPNAVTMDQKFQLKDKELKKLYELAESKSDTYPEILNDLKLNLVNK
                     SEQYLGEKINSEEKLLDYIKQIK"
     gene            30182..30766
                     /locus_tag="NBH55_000453"
     CDS             30182..30766
                     /locus_tag="NBH55_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508469.1"
                     /translation="MGLKQSVYIWLYPDNPMSIMADIFVGSILLCLLYLIFFHNLIGY
                     MILDWKGMFDIERKPFNNKQIYTTFAGGDLEEKEVDVTHEIGLALSGIQDADVLGSMI
                     TDTGYNAYEVFYLMYFIWRDSQDIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     NTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            30777..31028
                     /locus_tag="NBH55_000454"
     CDS             30777..31028
                     /locus_tag="NBH55_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508470.1"
                     /translation="MKKIIDQPTNRLIVEGVIFVTMYFTVGNGISNTIIQKDPQYLLF
                     SLYAWIVQMLWVSLLMLLFYWIVRVILLVLNKAKKKKDR"
     gene            31035..31757
                     /locus_tag="NBH55_000455"
     CDS             31035..31757
                     /locus_tag="NBH55_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508471.1"
                     /translation="MKATKLKLVLFSTAVLLTLSACGTSKSADNTKDTKSSSTVVTES
                     SSAQTSTTMSSMTENNAEKFQKYKDVNQGKKPEEVYNEFISSVKNDESYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIQEGFSYAPTLMHDITIGKPAYEYNAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTLAVVTPKKIDGTLYWDLMGMTTNTSDEMIESTSNYYM
                     DLGAYKSYEQSEPVMDDQSTIK"
     gene            31887..32630
                     /locus_tag="NBH55_000456"
     CDS             31887..32630
                     /locus_tag="NBH55_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508472.1"
                     /translation="MKEISASEAHISQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQELVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            32636..33001
                     /locus_tag="NBH55_000457"
     CDS             32636..33001
                     /locus_tag="NBH55_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508473.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKQRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            33017..33547
                     /locus_tag="NBH55_000458"
     CDS             33017..33547
                     /locus_tag="NBH55_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508474.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            33709..34074
                     /locus_tag="NBH55_000459"
     CDS             33709..34074
                     /locus_tag="NBH55_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508475.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     RKLAQSYLEEKAGVIKDKE"
     gene            34091..34480
                     /locus_tag="NBH55_000460"
     CDS             34091..34480
                     /locus_tag="NBH55_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508476.1"
                     /translation="MNEETELFLKKVNFLVEKYFLTKHKIMYLIKGLIGGIILWILTF
                     FSLFGGFTNYKKLVDENVKQISASESLRHQAEDLVSNPLFIGGMVEVIIISFVIGYLG
                     MCIFQIIFDLIRGKVFQEFKSEKINLR"
     gene            34510..34734
                     /locus_tag="NBH55_000461"
     CDS             34510..34734
                     /locus_tag="NBH55_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508477.1"
                     /translation="MEQTNHITEETRKFICLESFYSEGRYCNKGETYTAYPIEGGFKL
                     VFENGDMNFTTELFECVLETWSDVLLEVTK"
     gene            34787..35068
                     /locus_tag="NBH55_000462"
     CDS             34787..35068
                     /locus_tag="NBH55_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508478.1"
                     /translation="MAYENLQLVVAGVSGDIYLAKAKDGIMDTNYRRVATNDVLVATT
                     EWFIRNNKKLIQFEADENGRHSLFYTNDQKKADRIIAILKENDDGGQRD"
     gene            35049..35291
                     /locus_tag="NBH55_000463"
     CDS             35049..35291
                     /locus_tag="NBH55_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508479.1"
                     /translation="MVDKEIKGEIRLFQRENDDWGTEISKNLAEDIELRLSFISMIIE
                     SFAFELGIPQEKLAEILIKASNNSPQHFHIDLGTVN"
     gene            35413..35661
                     /locus_tag="NBH55_000464"
     CDS             35413..35661
                     /locus_tag="NBH55_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508480.1"
                     /translation="MSENHLKRLSKGTASMFDANLLIFKSLLEFEKQRVEDIAKYGNY
                     ATVPSVYNDLMKWFKVQTGLEYSQQNLVVKIEEYENHG"
     gene            35688..35852
                     /locus_tag="NBH55_000465"
     CDS             35688..35852
                     /locus_tag="NBH55_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508481.1"
                     /translation="MLLESDDPIAELDQLAERAKELEMISGAYRIISEELGQAVVSQA
                     LENYLKKQVD"
     gene            35936..36148
                     /locus_tag="NBH55_000466"
     CDS             35936..36148
                     /locus_tag="NBH55_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508482.1"
                     /translation="MPVKTNTEWLDICDELFDTKDKKELAMRVAYAIVQKDKYFAVPS
                     TDYDIEVIVDPITGDKKLRVVIECNA"
     gene            36257..36433
                     /locus_tag="NBH55_000467"
     CDS             36257..36433
                     /locus_tag="NBH55_000467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508483.1"
                     /translation="MDIFEVKPDSGKFIVLQEKRVIAIERTEIEAYKKIHELAEQYIE
                     DLNNNFQEFLTQNE"
     gene            36544..36765
                     /locus_tag="NBH55_000468"
     CDS             36544..36765
                     /locus_tag="NBH55_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508484.1"
                     /translation="MDIYEEVICDVEGKEEFLPCIKELETTVAITKVANAVSNETNEL
                     VGIHEAGNLVFSCLNSNKSALLALDGDLF"
     gene            37137..39377
                     /locus_tag="NBH55_000469"
     CDS             37137..39377
                     /locus_tag="NBH55_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508485.1"
                     /translation="MSEFEFLDKSIGSAFLIDPDLFGAWAQSSGYNWAFSDDMVELLS
                     QTMNWEAYKIKQFLDVVAQPIYTSADDHWSGRELTTLKELAEYNKRYKIPFQVSYMSD
                     DFSVIYSIGKESRYLIPNRHLEIKMNSDYTDYTLSELKALRQSDSEEFSALKNEVSAD
                     NFRRSDIEELKKKQKQQLEKQKKLIEDIEYKRVEELAIQEAKIEKMRQELYKKQQSML
                     AEANKMRFELEKKVEDLNTKIFYLETEINAIEGYFGESTTFVQLRSGQKSLPEEPVVM
                     FQKVRYLDEELGRYFSMREVEGTADTMLTFEQALSSRDDLFDLFSPNKKSISMIRISK
                     SGYGYTNGQMESTNMLEKYKKYHGETLAILLRNGDNLWICWLDENKISFKDDLFYQNK
                     VTEQASETKNMVKEIDWTHTRNLEERLKDAKKQKKAVRGEFASRYILFSILQGVLDNG
                     TMMTLPEKVNVIQAINQKSPYFIASVADGWLEDTRLPSFSELIDKYSFYKETGEDRVL
                     EAEYELNPDKFENMRDYPHKQNDDIYVLYRLRGWSPTSANYTYYNSDNRSNSESGRDL
                     AYSIDLKDGIYQINLVKQGKFQKQMFSRTFVGVDSTTGENVYDYYYERETEYYIRGQK
                     AYNKSDYWGDKAKRLTANFRVYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVP
                     MLNSMSRFLKEREEKELEYIKEIMPDFEMHEWQIALSDWKLTNKVRRITPFQAKRFLK
                     AMKFVQ"
     gene            39515..40312
                     /locus_tag="NBH55_000470"
     CDS             39515..40312
                     /locus_tag="NBH55_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508486.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYGESQNKPRKKKQAYVLKMVNGE
                     ELIVLGYSKEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYNSNRFDRLEKFCRPYTVLKTKKFNKTKRVS
                     IKLPTGQISLGEKDYLIKVGKELIIKVPERLFKENFEIRETLVWKEE"
     gene            40352..40534
                     /locus_tag="NBH55_000471"
     CDS             40352..40534
                     /locus_tag="NBH55_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508487.1"
                     /translation="MAVNRKLQVLITDDFGGAEWFDVQSIDFEEEVVVIENVYGEEYS
                     YSLEGENVEDLRLIGE"
     gene            40578..40865
                     /locus_tag="NBH55_000472"
     CDS             40578..40865
                     /locus_tag="NBH55_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508488.1"
                     /translation="MINKKTGKRIFSSKDEVIRDLSENFEPIELFFHQLNLEIEKLLK
                     EPTEGRINKVLKLIACKDSHAYMLYPKWYADKNIAEINDYLASIKERNAIN"
     gene            40902..41189
                     /locus_tag="NBH55_000473"
     CDS             40902..41189
                     /locus_tag="NBH55_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508489.1"
                     /translation="MAEVKFGSKEWFEELNSMIQFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            41280..41519
                     /locus_tag="NBH55_000474"
     CDS             41280..41519
                     /locus_tag="NBH55_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508490.1"
                     /translation="MKELEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHFPKVRFYN"
     gene            41647..41976
                     /locus_tag="NBH55_000475"
     CDS             41647..41976
                     /locus_tag="NBH55_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508491.1"
                     /translation="MKLEGSLQNRMMEGRTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELIKTKNGWKVLKEDTYFYIGDRE
                     KYYDPSL"
     gene            42029..42877
                     /locus_tag="NBH55_000476"
     CDS             42029..42877
                     /locus_tag="NBH55_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508492.1"
                     /translation="MFSQEEIISKSQPDKNGTITMKKKIPCYRCSGSGQYKFMSMGKI
                     TYGTCFKCDGDGFLVVTEKSFTPEYRAKLNALNKKRADRKIRITREKESQKNFDRYGF
                     EKGYIYVVIEENSYAIKDELHHQGATYDPLLGWFFKEKQEQYKTQKVFVEEFSVINDE
                     GIIELKLEEYSERQQEILRAKRREEGEFIGTIAQRLELDLKLTNTVTIDTQWGNVYLH
                     LFEDQNHNSLIWKTNKFLYYDEWDVGEIRKVKATIKAHDYYRDTKQTWVKNVKIQKNE
                     SEGIKV"
     gene            42874..43191
                     /locus_tag="NBH55_000477"
     CDS             42874..43191
                     /locus_tag="NBH55_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508493.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            43194..43427
                     /locus_tag="NBH55_000478"
     CDS             43194..43427
                     /locus_tag="NBH55_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508494.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIFQRFRLPVSE
                     LKKMMRLSAYLLMFLNVVTLIFVVIGVTFNLME"
     gene            complement(43544..44023)
                     /locus_tag="NBH55_000479"
     CDS             complement(43544..44023)
                     /locus_tag="NBH55_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508495.1"
                     /translation="MTDKTSDLPKLAPKTSRVKRKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            44143..45480
                     /locus_tag="NBH55_000480"
     CDS             44143..45480
                     /locus_tag="NBH55_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1508496.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVSGDVAKNIDDIYKFMNGEYGLSGATIAGILGNWKQESGNDPRTVEG
                     YYGDGQREVEFIKQFTDGLMGIGYGQWTAGRHTALVNWAKKNFGGAWWTTEAQLDFMF
                     KGDGGFVAILKDYALNASDDVVANAVAFHKNWEISADSESAVVAVRGGNAKEIWEYMK
                     SKGMDGAKDVSKINKIAGGTSTGGGSNNSSSSASGTVEDACATTDDANSGSGSMGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            45503..46030
                     /locus_tag="NBH55_000481"
     CDS             45503..46030
                     /locus_tag="NBH55_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508497.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSDKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            46017..46580
                     /locus_tag="NBH55_000482"
     CDS             46017..46580
                     /locus_tag="NBH55_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508498.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLENLDSYVRAE"
     gene            47003..47740
                     /locus_tag="NBH55_000483"
     CDS             47003..47740
                     /locus_tag="NBH55_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508499.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(47757..48173)
                     /locus_tag="NBH55_000484"
     CDS             complement(47757..48173)
                     /locus_tag="NBH55_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508500.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            48565..49701
                     /locus_tag="NBH55_000485"
                     /pseudo
     CDS             48565..49701
                     /locus_tag="NBH55_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
BASE COUNT        18030 a         7240 c         9442 g        15109 t
ORIGIN      
        1 acttttgttt tgtgttaagt ttctttacat tcctacgccc tttgtgtgta aaatttcttt
       61 attcctccca cgctccgtgt tattttatat ttcctttttc atgttatttt tcgtcagaaa
      121 ccgatctagt ttttacctat tcccaaaatc catttattcg ttatttttta acctgattaa
      181 ggataattat ttgatgctta aattagctaa aaaggcgatt agttaccgat ataatcaagg
      241 tattaaagct gaattaggcg gaaaggcaaa atgttcgcaa aatcaaagta tcaattatat
      301 tgttttttta taaatttgta agtgaaaatc tgaaaaatcc tctttaaatt ggtataggca
      361 ttaaaattac atacaaccac tcttaaaagt atgcgctttc atcgttttat atgatattaa
      421 tatcaataaa taacgatata aaaaccattt atcataatgt tttttaagga tataaattga
      481 tctaatttaa aaaaataatt caaaatttaa aagttttaat attacaagta aaattatgtt
      541 atcgtaaaat ggtataaaat agcttaatat tctatttttg ttattttatt agcaggaaaa
      601 tctaaatatt tttacaagaa atcgtagaaa tatattggtt tcgccttcag attaatactg
      661 gatcatatta atctgaatac aatatatgaa ctaataaata gcttaacctc gctattttgt
      721 tttttctgat cattatatac acagcattaa caaatagaat tttaaaaata aaaaaagtaa
      781 gacaaaaaaa gtatcaacat aaacatatac atatacacgt ttatgttgat acacttaatt
      841 tgtcctttaa aaagcactga tttttaaatt gcgttatttt ataacccgaa tacagtctgc
      901 ttgttaggca ttgtccttaa attacatttt tcataggtta tttaatcatt taatctgttg
      961 attattcttg atctaaaact tagcttaaca aatatataat cagccaaccg cctgataaaa
     1021 atgcgatcat tgctaatata taagcaaaga taaaaagatg ttcgatcagc ttaaaccctg
     1081 atgcgattaa gcagaccaag taaaatagaa attctcttac tgataatggt aattttctaa
     1141 ctagcatact cataattaac accccaaagt aatattgtat tttgaacatt ttacatccag
     1201 tacagcatca atcaaccatt gccaatcaaa aatacaaggc ttcattgaag tatgtttttc
     1261 agatgaaaag atataatata attcctttgg aataatattt tcagttgttg ggatcaagct
     1321 aaatctaatt ccatctaact catagctacc agctttctct ccattaaaaa tttccttagc
     1381 actttcttct aaagaatcaa aatatttttc gacagcaacc tttgaagctt ttactattga
     1441 agcatttccc ttttcgataa aatctgcttt tgttttaccg acaataagta attcttgatc
     1501 agtaaaatct tctaaaataa tggtagtgta attcttattt cttctcaatc gcatcaggat
     1561 cactccttaa tataatttat gtttttatga tatcataata agataaattt gcaagtattt
     1621 gtttacacct ttttatttac actttattat aattaattat acttttatga ttgcattttt
     1681 ttcttataaa cagttgcaac taatagtaaa taggtgtaaa ctattttcgt accgaaatac
     1741 cataaacaca aacagaaaga ggtcatacca tgaaagaacc attattgttt tccgttgact
     1801 tagggaataa atcagtaaaa gaaatgagta cacacgtttc tccactctca cttccctctc
     1861 gtattttgaa caaaaatatc attaattcag gacgtttttc taatataggt tcctcattaa
     1921 aacgatcact aaacgtttct gaatatagcc tagtaaacac tactgaaagc tattattttg
     1981 ggaaggatat acacgagcta ggaaaagatg actacgtgat agaatcacta ggttttggga
     2041 ttagcagata caagcaggaa acctaccaaa atatcctatc tttctcttta gctaacctag
     2101 cacgtttaga agatcaatct gatgacgttt taaccgtcaa tctagtttta ggtctgcctt
     2161 catccgacta taccgaaaca atcgtcaaat acgtgatgca acaagccaca cgacaacatt
     2221 cagttgaagt tgacggtaag gcttataata taaacgtaga taccgtttgg gtactgccac
     2281 agccaatcgg aacgctatat aacctcctac ttgctgatga tggtagcgta caagatgaaa
     2341 ccttgatcag tcagaagatc ggttttgttg atattggtgg tggcacgaaa ctttttgatg
     2401 ttgtccaaaa ttttgaaatt gacgaaaaca atcgtgaaca aaaggatacc ggcattttta
     2461 ctttatataa tcgtatcgta aatatgcttg accttccgat cagtcctaat gctttccaac
     2521 ttgaaaagat tatccgagaa ggcaaagaaa aaggcgtata tctatttaaa ccaagcggaa
     2581 atgaaagttt tgatatttca gaagtaattg aaagagaaat aaaaacatac acaaatatgg
     2641 ttgttgcaga agttttagct acattcaaaa acttcacgac cttagataaa gttgtcttta
     2701 ctggtggtgg agcttctatt atcgacaaac aacttgtaaa agataaaatt ccgcatacct
     2761 tgtttgttga agacagtgaa ttttctaatg taaaaggctt ttacaagttt ggtttaactc
     2821 aataaaacag cgaaggagat caacatgaat aagggaaaat cagagcgtta tgtagttata
     2881 atcaatagag aaaaggttag cgagttacct ttagcagagt ttcttgaatc cgaatatgaa
     2941 acactaggcg ttaaatactc tcagacgatc aaaaaagctt tgaacctata tcggcgtgta
     3001 aagaacaatg aagttacaat tattgaagca ggcaacgata gaaacgatag caaagtagca
     3061 gaagtaaaga ataaaccaac tgatcataac cctattcaac aagagcatcc ttcaacggtt
     3121 acatccgatc attcagcaaa gaaagaaaag ccaatgctaa aagggttcaa tgcaccaagt
     3181 aacaaagact tactttaaaa tgaaaaaggc gaggttacaa tacctagcct ttttattatg
     3241 tacaaatata aacataaaca tatacgtata catgtatata aacatgtata cgtatatatc
     3301 cataaacacg tatatgaaat ttagttaagc tatacctaac cgcttattcc aataaaaaaa
     3361 gtgatcagct ttaaactgat cactcaccaa aaacttcatc atacaagtta tctatcattt
     3421 caccaagtaa agctgaatct gatcgaattc ctaacttttc gctgatttta gatagcttct
     3481 ttctttgcga tggttgaagc gtgaattgat aatttttctt cttttcagaa ctatcacttt
     3541 ttagtttagc taccaaagaa cttgttgttt ctttaggtgt ttgtttaggt tcttcaactg
     3601 cttcaaccac ttcaggaact tcttctactt cagcttcagc gaatttttta aatctatttt
     3661 ctttcacttc aatcactcct tattcttgtg ccgtatcaat tatcttgttg tatagcgtaa
     3721 acgtgctttc aaagaaatcc ttattcttac gatagatatt cttatcctta gcgaactcag
     3781 caatattctt accttctaag ctagttgcat taagcaattc cttctcttgt acatatccaa
     3841 gataattaga taacgttggt accaattcta ggaactcacg acttgaatta gtattatgtt
     3901 tgatcctgct accgatcgta gcgtatttag tttccatcag tgtttcacca gtcttaatat
     3961 caatcaactg ttttgctagt gcatcaatat ctttaccaag cttcagcaaa gcttcaaaac
     4021 catctttaga tggatcagaa atgcctacga caagatcaga tacagcccaa gcattttgag
     4081 ctagtagacc tgcatcatta tgagtatcaa tcagaattag atcatacttc tctttaagct
     4141 gttcatagtt aacaacgatc catctaaaca gtagggttct gttgttaggc atatcacgca
     4201 tttgcatctc aacttgtttt aaatcttcac ctgctactaa aatatccatg ttctcattta
     4261 tagctaaagg ttcagctgat ccatgaagat ataaatgatt gattgtattc tctcttttcg
     4321 ctttactgaa agcatcagga aagcgttttg ataggttttg cgacttatct tcatcaatat
     4381 acagcacctt catacctaaa ttttcgacag catatccacc tagattgtag attgttgttg
     4441 ttttacctgt tccacctttt tcaatgtttc cagtaatgat tgacaacttc ttttccatga
     4501 ttaaacctcc cttattaacc ttttgtaact ccattataca ctataaacac gtttatgtat
     4561 atgtttatat acataaacgt gtttatattt atatttgctt ttatattcta taacataaac
     4621 atatatatgt atatgtttat gtttatgtat atgttcatat atataactat tgcatctgtc
     4681 atacaatcgt agtacaatgt aattgaaaag gagttgataa actatgccag taataagtat
     4741 tcgtgtatct aaagaagaaa aagattttct tgaaaaagtt gctgaattca acggagataa
     4801 catttctgaa tttatgcgta gtaattccct gcaaacagca gaaacgttag tagactttgc
     4861 cacctacaaa gaactaatga aagagcatga ggaaaacgat caaagtattt cccacgaaga
     4921 tatgctaaaa gagttaggct tataagatga gttaccaagt aagatatgaa cgtgaagctc
     4981 aaaaaacgct aaaaaaaatg gataagtttc aggcaaaggt tattctaaac tggatcgaga
     5041 aaaatcttgt aggcactgat gatccaagaa agcatggtaa aggtctaaca gctaataaat
     5101 caggctactg gcgatatagg gtaggtgcat accgtataat agctgatatt agcgagcatg
     5161 aagtgacaat tcttatattg tctattggtc acagaagtga agtttataag taacagacaa
     5221 gctgataagg cttgtctgtt tttttgttaa cccaacaaaa tcactgttta cgatcccact
     5281 ttacctaaaa aagaacaata tcctcttgct attatacaca ccagtgtgta taatataagt
     5341 gtaaacaagc taattaactt tcaaaagtag cgatttttta tgggatggta tggtgcttat
     5401 tcatcagcaa atgaagtaat cgaagaaaag aaagggtacg ccaaaaatgg tttggaaatc
     5461 gtagatacca aagtaacgaa gacctatgga attatccttt tcaaagatgc agaaggaacc
     5521 tacgatattg acagcttcat ctttcgagat gaaatgtaca aaccaatcta cttcttaaat
     5581 gacagtcctg cttactttaa gaaaattcca aaaaaatggt tgaaattctt tgtcggtgat
     5641 caagaaaaaa tggttaagcg acaactagat agaattaaag aagaaagcaa taaaccaaaa
     5701 cttgatgact tattagttgc aggtaaaact tactcaatct gggatggatc gtatgtcgct
     5761 gtttatcagt ataaactcaa acgtagcttt atttttcgac tacaagatgg atcactcact
     5821 cggttcactc gactatcagc aggagatgtt aaggaagtag cctaaaactt aaatacataa
     5881 acataaacgt gtatacgttt atgtttatgt atatgtatat gaatatcaac gtattaggta
     5941 cgaacgcttt taaatgcttt tctgggcttt gattttaggg tagggctaaa acatcaccat
     6001 ctgatccaaa acagctcaga aacgcattta aacgtatctg ataccaatat tacagaagga
     6061 gtgtatcgta tgattaacct atttctttta gtgacagcta gtacattatt ctttactgga
     6121 atatcactaa taatttccta tttgatctat aagaaagccg aaacttacac aagcaaagca
     6181 aacggactag ccgtttcatc ctttttaact ttgctgactt tttttgttac tctatcgttt
     6241 tcttttgttt agcacagctg atccatcagc aaaaaacctc tctttacatt atacacattg
     6301 gtgtgtataa tgtaattata aacaagctaa ttaactttta ggaaggagtg acaatatgct
     6361 aacagctaca ttacagcaac ctactcacgt tgctattgca gaaagctata caattaatgc
     6421 aggggtcgtt aaacagctga tcaaggatcg agaattcaaa aaatttaaca gtcgtcaagc
     6481 gtataacaga cagttaaaga aagctattct aaagcacaaa aattccttca tcgggaaatt
     6541 ggaaagtgtg gatgttaaaa ctgtttcaac ggcattttca gttgttgaag tgactattga
     6601 cggtcgcttg atttctctat ttgcttttgg aattaattga tagggagtgt ggactttgga
     6661 aggtaagact tttgcagaaa tttggactga atttattgag gtaccaactc gcattgatcc
     6721 gataacttct gaaaaggtat tggatgcaga cttctacaat ctcaaagcag gtactagcct
     6781 tgaatgcgtt tggatgtatt tagaagagca tttcaaaggc gtaacgctcg gtcacgtctt
     6841 ataccacaat ggtgccgagt gggatgcaat tggaaatatc catttttctt aatacaagaa
     6901 aggagaacac aatatgaatt ataccgatga agaacaaatc actttggaat acgttttcac
     6961 aaatgaaaaa ggggaggaaa ttgaaaaagt cttgatcatt tcatcaaaag acttggagaa
     7021 cgctttggct tatgaaccat acgaatcaac cgtaactgtc aatgaattct tagataccta
     7081 tacttctaat gatacttggg aaattatgaa tatttgcaac gctaataaca ttcaatttgc
     7141 tgaaaaaacc aataaatcat tttattagtt ttgcaataaa acatcctctt tttctattga
     7201 ttccatacac aacaatgtgt ataatagatt ttgtaaaggg aggggagcta tgcctaagac
     7261 agcaagagaa attgtaaaaa ttctcaaatc taacggcttc aaaaagactg gacaaactgg
     7321 cagtcatgca aaattttata attccaagac taaaagaacg gtcgttgttc cgatccacaa
     7381 tggtgatgta ccactaggaa cagaagctaa tatctggaag caagcagggc tttaacagcc
     7441 ttgccttcct atttggtatt aggcatacta tcctctctta cactagaaaa ggagcgagga
     7501 aatgaagaca aaaaatattt tttatccggc aatcttcgag aaagaaggag aagcttataa
     7561 catcgttttc cctgacttac cggaaatatc tacctttgga aatagtcttg aagaagcata
     7621 tttaaacgct aaggatgctt taggattagc gctatatagt gtaccagatg cagaatttcc
     7681 taaaccatca gcagtggaag aattaacttt gaaagccaat caagtggtag taatcatcca
     7741 actaaacatt aaacttttcc gaagaagctt aaatacaaaa acaattagaa aaaatgttag
     7801 tgttccggaa tggttagttc ttttagggaa ggagaaaaac ataaacttct ctcaactact
     7861 tcaaaaagca ctagaagaag agttgcttga aaagtaaaaa ggagaccgaa cctatgaaag
     7921 ctacacactt aaactatttc tctcgaacgt taatcaataa tctattaaaa gatacaactg
     7981 gcattagcgt tagtaccaaa cactttgcca atgatctaat tatttttgag gataataact
     8041 tgcgaattga gatgtttatt gaacaggcaa cccctaactc aatacctgaa ttctgctata
     8101 cgcacactga aaaaatgaaa catgcaaaat cagttaaaga tgttgaagca ttaagtgaat
     8161 cactaacgaa cgctttaaac ctcgttaaga aggcaaaatt gttatttgaa tttcaaaccg
     8221 actaccacaa tgagttgctt gctcaatctg aaattttaga ttaattgaaa caggagaaga
     8281 gaaagatgaa acaaacacaa acagaaccca ttaacgatac gcttgctgat caattagcag
     8341 gatattataa attatttgag atgagtaaag cacagcgctt gatccactat ctttggaaat
     8401 acaaaatttt gtttcttttt tgggaagcaa cactaacatt ctcagttctt ttttataagt
     8461 cagattgtta taatcaacac ttagttttag ggagtttttt gcttatctac accattttga
     8521 tgcttaattt atatacctac atcacacttg aagaacactt agtaattagc gatgcaggga
     8581 tcgagagaaa catcaaaagg gtatctttca agatcacgtt aaacaagcct accgataaaa
     8641 agctctcaga agcgttctga gagcttttga cattttcttg tataatttat ccaaacacat
     8701 tacaaagcac ctaattcgag cttatatcaa gcaaatttgc tatttattcc tcaatacata
     8761 tctaaaaatt acttgatgga aaactagata catggtaagc atgaaaagaa aatcaatttg
     8821 agcagatgca gtataataca tagcaattta aaagctctca gaacgcttct gagagctttt
     8881 aatatattct ttgataattt gtctttctta aatccatacg tcctaaaaca agctgttttt
     8941 gcttaatatg atcatctgag agacatcaaa tcaacttttc tctcaacaaa tcattcaaag
     9001 ctttcgacag attaatattc tcttcagccg ctaaaacatt taaatagtgt tccacagtca
     9061 ctgattttcg atcgtaattt tgcttgtttc gagctttagc aactttcgta ttagctttta
     9121 cgacaaataa attaacattc tcagatcgct tgattcttgc atttaactct tgaacaggcg
     9181 taggcgatgg aatttctttt tcttgcaact ctaaaagcac caagtatcct tccaatactt
     9241 cttgcgccat ctcaaaagca tcttctaaag tttcaccatg cgtatataag tctggaaaat
     9301 ctgggaattt aactgcatat tcatttcctt tccattcgaa tactgcacaa aaaaagtatt
     9361 tcatcgcatt gcacctctct aattaaaaaa taatttttgc tttttagttt caaaaaaaga
     9421 atcgcctacc aaaaaaattg ataggcgatc acaagctaat taacctctaa aggagattaa
     9481 taccttgctc ccaaggcact acatatctta gcatacgtct gaagcatctt caaacgagta
     9541 agatatattt tgcattaacc taattttttt ctctataagg tttttcatca aactcactag
     9601 aaatattaaa ctggacagca aaattgtaat ttctgccttt tctaacttta gcaaaatcaa
     9661 tcttcagcat tgatttttca ttaatttcct caactgattc ccttaaaaca cgtctatcaa
     9721 aattcgatat tgttttgtac tcattttctg ttttcgtaat ctcacgaatt ttaactattt
     9781 caatcagggg attcttatat atttcagctt tctcaaaatc tcctttattt ttggcgataa
     9841 gatatttttt ataatacatc gccaaatatt tgtataacac aatgctatat tttttttgaa
     9901 agcttgctaa atcttgaaaa acaaaatatg gatatttctt tttgattgaa agaattaggg
     9961 gaattacttc ttgcgagaaa ttgaaagtta taatttgttc ttctaagtcc caatgaattt
    10021 gatcaatagc agatactgta gcataagtct tatattcttt atctaagttc gatatttcat
    10081 aaactgcttg tctctgaagg gtgttaacta tattttttag ttctgtttca agattttgat
    10141 caacatccat gtatttgatt attgctttaa caggtaaaac tatttcccct gttttcttta
    10201 atttgttagg agagatataa ctgaacagca attgataaag ttcatacgac tttaaatcca
    10261 ttttcatgtc aattgttgct aagaactcct cgttgtactt tgtcagttca tcttttttca
    10321 aaagcttttc cttccttcac gcaagttttt cagttgttaa tcataaaggt tcacataagg
    10381 taaataactc gtaattgatt tttcgagata tttaacagta ttttttattg tgtaatcctc
    10441 cttatgagta gcaacgtaag atagatgact gacaactgat tcaaatcctc tatctttgga
    10501 taaacgatca taaagcggat aaactttatt ctgaagccga ctcataaatt tcagatcgca
    10561 taagtcggca gaactgatca aaaagttttt gattagtaat ttagtgtatt tgctattcat
    10621 agctttcaag tacgattgat tcattttctc tgttttatct tctgcacctt ttaaataagc
    10681 agggtctctt tgttcttgct tataaggtac cggtactgac atggtacgtg taagcaagaa
    10741 ctttatatct gtataagtat gacccttctt gataagctca tagtcaattt taaaatcagt
    10801 gtatttattc agttgggcaa tcggttcttt aattacacgt ctttcaaagt ctcgaaaagc
    10861 tttatatttt ttctcaatac tgaatttctt tcgtaactct gcaattgaaa aaacggggtt
    10921 ttttaatcta tcaagtgatt ttgtactcca tgttaagtta tctctatttg cttcatacag
    10981 attaaaatct ttagtgaata attgaaaaaa actgatcgca tatttgcttg tcaagttaac
    11041 tacttgatca aatgtatatt gtgtaaaatg attttctaag ttcgttaaca aaggcataat
    11101 tgaactacta aatttaatag atactgcatc agaaaaatca ttgaattcag taatttctag
    11161 cggtgagatg actttgtatt cagtcttacc gttaggttta gttctttgaa ttttaatgac
    11221 agattgctga tgtaattttt caataacttg ttttaatctt gaatgttttg aagaagatga
    11281 actagaagac atcaacgcaa ataaagcctt tttagatact ttgatggtat tattaaaagg
    11341 cggattttta gggtcaagat gtcctatagc gtaaagataa attttttgtg gcatagcatc
    11401 catttcaaca atgctatcca caagttgatt cgataaagta acagttaaat caaacatttt
    11461 tagattgctt tcggtaggtt caaatttcgg gtttacattc ttttctgtta ttttcatacg
    11521 attgcctctt ctttctaagc ttggtacgag cttaaaaact tggaactggt acgattccat
    11581 tgaataagta tagacctgtt ttcgatacga tacaataaga aatgtatcgt aaatttataa
    11641 cataactcaa tctcagtaaa acgcatttta tttttattaa tcaaatttca ttgataagac
    11701 agcagaaatc gctattttag aacctttaca aaataaaaat ccttttcaaa aaaaatacgt
    11761 aattataaaa attcacaaaa ttaaaaaaca aatatttttt tagtttttag aaataaattc
    11821 acatactttg tctttttttg tatatattcg gaataccaca tcgctttttg tccacattca
    11881 catcgctttt tgtccatgtt cgcatcgctt tatgtccacc ctaaaatcta tttaagattg
    11941 atataaagcc tttttggaga ttgctaaagt tttaaagatt aaaatatata ttataaatat
    12001 aaataaaagg ggaagctgaa gcttcctcct tttccttttt tttaattatt caaaattaca
    12061 caagcaaaat aaactgctag atagtgttag ttttggtttt aaggttttta atttttgaga
    12121 ttttgaaata agagaggagg aatcctcctt tattaaatat ctgttaatat atctgttaaa
    12181 tatctgttaa tatatctgtt aggggggtct taaccccttg ggagagtaag tattgaaggg
    12241 gtatgaaggt aacacttttg gacagtaaag gtaacaaaag tggacagtaa gggtaacatt
    12301 tttggacagt aaaggtaaca aaagtggaca tttgaggtat caaatcagga taagacgtta
    12361 ccttcggttt tcttgatttg aaaatcgaaa aagtagaaaa ctagattagt aacaaatctt
    12421 tgttgactgt tacctttagt tgtgatttaa tttaggtaac aagcagaaaa aaagcgtttc
    12481 ctttaattta gtaccaaatt aacatgatta aagggatgca acaagaagat tggagagtaa
    12541 tcaatgtctc aagaagttac cgaaaacgtt attgataatg atgtagattt agtatcagat
    12601 caaacaaatg caattgtgcg ttataacaat ggtttgaaca caacagcttt gcgaggattt
    12661 aaacctgtcg aaatggatgt gttttgggct attttatcaa aaatgaaacg tcaaggaaca
    12721 gcagaagtaa cttttgattt caacgtcttt cgttatttgg tcaaatacga cagaagagaa
    12781 aaggacagtt tttattctgt gttaaaagct ttaagtgaca aactaggtac tttaacctac
    12841 cgctttgaag attcagatgt ctatgaacag ctgtggcttt ttcaaaaatt ccgaatagat
    12901 aaacagcaag aaacagttac aatcaaggca aatgaggagt ttgaatttat cttaaattca
    12961 attggcaaaa actttactcg ttttgaactt gataacttta caaaactaag aagtagctac
    13021 acaaaagagc tgtatcgtca tctcatgcaa tatagaaata aagagtggcg aaatggctat
    13081 tgggtagtca aagttgaaga ttttagaaga gcgttaagta ttccttcaag ttacagaatg
    13141 acaaatattg acaaaagaat attagagcag gcgaaagaag aatttttagc tcctgatgaa
    13201 aatggtatac aggtatttga atcttttgat tatgaaaaaa tctatgcgaa gaaaggaaga
    13261 aaagttgatc ggctcagatt cacttttagt gaaccagaat caaacttgcc atccatttca
    13321 atgcataatt ggttagaaga agactgaaaa gaagcaggtt gtaattttaa tacaacctgc
    13381 ttcttttgaa aaaagtatgg gtaacacttt tggacatgtc aataataaat atattttctt
    13441 tcgtcttttg cctaactaaa ggtaacactt ttggacagta tagttttgta aactaattag
    13501 atcgggttta atgagatata tcttagcatt tatgtaagaa tgaaggtaac attaatggac
    13561 atgtcaactg tttgaacaaa aaaatataga ctatttggat ctgatttttc atttttccaa
    13621 acgttaaaaa atagttagtt attttattaa tagatagtca tccttaatgt ttgaaatagc
    13681 agaaaataag atattgtttg ataaatgttg ttgttataag gaaaacttga atttgttaat
    13741 tgatttattt atgttattat gatactataa taacataaat gtgttacgcc cgaacctcat
    13801 tttacaaaaa tgaaggggtg agacaatgga tgctattaat tggcatgaat ttgttaacta
    13861 tatggagaaa ctactagatg atctgaaaag ttttagcatt gctgatgccg taaaacatgg
    13921 gttgccagtt agtacaacga tgaaaatgcg agggttgaag gttccggatg aaattatcaa
    13981 gctgaggact aaaacaatca ttgaaactgc aaaaattgtt gacagtatga accttgattt
    14041 gaaaggcaaa aaaacaactt atgagaaaga ctttgtacgt tttttaaaaa atgttatcgg
    14101 aaaatatcta gggaaaaagt cttttactga attgagagac gaagagattg taccaactcc
    14161 aatggttagc aaaattcgca aaaacaaagc cgagggaaat tactatcttg gcacaatagc
    14221 aaaaatcgtt gagtatttgg aaaataaaga gcaaaagaaa ggaagatagt tcgtgtgata
    14281 aatatgaata gggaagaatg taccgaaatg ataaaaaaac ccgaacctag aattaccgaa
    14341 aaactgactg attgcttgct cttttacgaa agggggcagg caaactaatg gaaaaagatg
    14401 tcaattcagc atccactaaa gatcaataca atatcattgg aggattactt ggtgataagg
    14461 ataagagaac acgtcatgga ttacgcatta tgcttgcgac atttctaggg gtgtttcttt
    14521 tattaacagg cttgggtagc tacatttatc aattaggaaa ctataattat tatcttgtca
    14581 acaatacaac accaatcggc tcatcattga cgtttgctcg atcaggagca gagatcacta
    14641 tctcagatgt ttggacagat aaaactagag atgtaacggt ggtcaaactt ggttactcag
    14701 gaaacgcaag aaaattgcta tctacacaag gtaaaaatta taacttgtat atggcaacaa
    14761 aagaaaaaga tcgtcctaag aagcttgaaa tgagttatgg gatgttaagt accgatggtg
    14821 atgctttctt gttcctaaaa ggtaaattag atgaaagagc ttatcaagtt tttattgcta
    14881 accaagtatc actaatttca ggtgattata cagctgatga tgataccaaa gtcaatgtga
    14941 atgaatcaga ttcagtcacc aaggcacttt ctcagtattc tatgaatgat gttgataatg
    15001 aaggagtaat ttcttttggg aaaaaagcaa aaccagttaa tactcctgat aatatcaatt
    15061 tcagaataaa tccatactca gatacaacta acgtatatca aggaagcttc cttaccaaag
    15121 atggcgatat tgattactca aaagttgttg ctgtgacaag taagaataat gttgtaaata
    15181 agctagaaga ccagttaaat gcttcaaata ctaacatcaa gaaaatcgaa gccacaaaag
    15241 ctgagtatct tgaacgttta caacaaaatc ctgacgataa agattcgcaa gaaagtattc
    15301 gtcaactaga cagtcgaatg gagaccgaaa ttaaaacaat caatgataca caaaaattga
    15361 ttgatcagta caaaaatgct gactttaaca aagattcttt tggagatatg aagacaacat
    15421 ttaagtacct aaccctgact gactagaaac aaattattct tattttagag gaagtgaaca
    15481 caaacagatg gaaaatagta ccgagttctt taaaaatatt caaaccgaat tcaatcatgc
    15541 tctcacacta atgaaaccac cgaaacataa gttagtgggg cgtgatgacg aactccatga
    15601 gttgtcactg atcttaaata gaccggaaac accacaagct atcttgttag gtgaagcagg
    15661 aacaggtaaa acagcactgg caagtgaatg ggctaaaaga cagcgacaag aggggaagaa
    15721 agtacaagta tataaattat caatcggaca attgcgagtt ggaggagatg acgtcctcca
    15781 aacacgtttg gaaactataa ttccaagatt gaaggaatac gaacgaattt taaaatccga
    15841 ggacaaagat aatgaatttg tcctctttat agatgaattt cataaagttg tcagttcatt
    15901 tgggagcggt aataaaattg ggggagattc gctgaaggaa cctttagcaa atgaatcact
    15961 tcagttgata accgctacaa ctagaaaaga atatgaaaac tatatcaaac gagatgatgc
    16021 tttaggtaga cgactacaac cgttagaact caacgaatta aaaagtgatg tcgttgtcga
    16081 tatactaaga caatggttga atacatcatc tgtcaaagat gatcctgaag cttccttgaa
    16141 attatccaaa aagattcctg atgaattgct tgaagaaatc gtacaagcaa acaagcttta
    16201 tcgtgaggat ttatccgaac cggctaaatc aattgccgta atggaagatt tgattgcgaa
    16261 ggatcgtgta gatggcaatg cgtataatcg agaaagcctg ttacaaatat ttggaaaata
    16321 taatattcaa ctagattctg attttgatat agcaaaattg gaagaggagc taaagtccat
    16381 taaagggcaa ccagtagcgg tatatacggt cacgatgttg tttaaacgaa tgaaacttgg
    16441 atttgatcgt tttaaagggc gaaagcaacc aaaagcaacg gctttgttta caggatcaac
    16501 aggggtcggt aaaactgcga tggctaaagc tgtagcaaga gggctatacg gtgatgaaaa
    16561 taaagtcctt ttagtaaata tgccagacta taaaactaaa gatgatgcta tgcggtttag
    16621 aaagcttatc ggaacacatg tccggcatga tccatcagca atcattgttt gtgatgaata
    16681 tgaaaaagca ggcgtagaaa cacttgatgc ctttttatct attctagatg aaggaagaat
    16741 cagttttact gaaagaggat gggatgatcg agaagttaat gataccgtct cactttcaaa
    16801 tacgatttta tttgcgacaa cgaacgcagg ggctgagatg tacgattcga tgaaccgata
    16861 cagctcacta tctcgtgttg ctaatcagga aaattcaaat gagtacagca ctgagcttaa
    16921 aagagagtat cgaaaggtag cattagagat taaacaagct ctaattggtg aaggtctaaa
    16981 acccgaagtc atcaaccgat ttggacggat tgttccattt tgggctttat cggaacaaac
    17041 tcttttggaa attgcacaca atgaaatatt gaaaagtgct gaattggtaa gaaaacactt
    17101 aaacgtagac ttacgccttc caccacagaa aaaacatttc tatggtcaag aattttatgc
    17161 agatgatctc tccatgcact tagtttttga tagagcttca caagaagatt ctaaatcagg
    17221 gggtgcaaga aatatcttgc atctggtcga atcagaattt gaagaagcaa ttactgaagc
    17281 aataattacc tatcctaacg ccaaaatatt tgacgttaaa ttgagcaaag caggagggtt
    17341 tgaagggaaa ggcgatacgg atgcagaaat tatcgctaaa ccttccatcg ctgaaagtaa
    17401 agaaaacaag tttgtgtttt cggttggagg ggtgtagcta tgaaatttaa aaagtcttta
    17461 cttcaaaatc gtaaaattaa gcgatgggtt gtaagaggtt tagttatcgt gggggcactt
    17521 gcagttactt taggcgctcc atttggaatg aatgatccaa cgtctgatga tcaaggaaat
    17581 aaacaagaaa cagttgcaaa tacgaatgtg gcttatgctg aaagtaaacc ggcagaaaaa
    17641 ggggtatgga cactatatgc tcgtatcctg atgggagata aagaggtcaa tgatagtgcc
    17701 actaaaactg gtcaaatgaa tttacttggt caaggaggta ttagtgctga ctttccttat
    17761 tctgatattt ccaaacaagg taatgcaatt gccggtggta acgcaggtac gcaattagca
    17821 tcaaccttag cgacttataa ttactatggg tatatcgaaa cagtttcagg aaatgcgatt
    17881 gcaagtcagg catcctcttt tattggtggt atcggtagaa cgcttggcag ttttatcgga
    17941 tcaattagct tgatactttc taatattgct gaatccatca atgaactcct tggaaaattt
    18001 ttcatgagta tgaatatatt ttcacttatg ggttggggta aaggtggatc ggatgtttct
    18061 aatccggtag ctaaggcgtt aactagctta cttaataatt tgggattaaa caatcaatta
    18121 tttaaagcga tagcaagctt atctatgctc ttttttacag caatactagg ctatcaagga
    18181 atgaaactgt tgtcgaaaga gggtgttaac tcaaagggat tatcaccagt acttgaaaaa
    18241 tggattgttc gtatttttgt attgtttgtt tttctaccag ttgcaggatc gttattggga
    18301 gtttatatca atggtattca gtcagcaaaa gaagatggaa aattaccaac aaatgttgtt
    18361 ggtgattaca tcattaatac aagactttgg gcttctactc aaaatcttgc tccatcatcc
    18421 gatactggtg ggaatgtgcc aaacggaaaa ccaacatctg aaattaaatt tgttgacact
    18481 gattatgaac catctaaaaa atcagaaatg atcagaatga tcaatactag cagttacgct
    18541 agacaaaata atgatctaga ttccaattca gtcggcatgg cattgttaaa agaatggtca
    18601 agtaatgata cgtttaatgt caatacttat gccggtgatg tgaatgtagc tgaaaatgat
    18661 gcttatatgg gaagagcgcc tgctgtttgg acaggctata atttagggga tggaaaagaa
    18721 ggaaatgcaa ttaagaaacc taccaattta aatggttata tttggtcagc taatcaaaac
    18781 gttacagacg acaatcgcaa gcctgatgga aaaggaacaa cgaataactt tgatgctact
    18841 caaccttggg gtgttgctaa caatgcaacg tttagcacac agtcggtagc gttgatgctc
    18901 caatcagcac tggatacatc aggggctaag ttttatgcgt ataatatcgc tccatcagga
    18961 attcaaggta gtgctaagaa tatgtcaaca gtaaagacag aatggcgtac agcttcttta
    19021 gttggtcatg gagctatggg taaattcggc tcatggttag cgatgaacgc tgaatcaatc
    19081 gtattagcag tgctttatct agcttgtgct tgggcgatgt ttaacgttaa tattgtaaaa
    19141 gccttcaaac gtgagtttgt gcgagtttgg gaagtagtct taacagggtc tcttgccgca
    19201 ggagcagggg ttatggcaat ctcattagga gtcgtaacca catctcttat cggattacaa
    19261 ttacctattt ttctgatcac ctttgttcgt gcatttggag atacggctgt aggattcatc
    19321 cctaacacat tacttggtga tggtgtagca aatattgtac gatccgctat ggtcttaggc
    19381 gtaggatacc ttctagtctt taagaaaggt gaaaaggtta ataataattt gattaatatg
    19441 cttattggat taccacttga agtagcgttt ggttttgacg aacgtgctaa gttattattt
    19501 ggcaatagaa atgatgctgt tcgagttgtg gatggcgttt ctaaagcaat caaaaaaagc
    19561 caaggggcta aaaatcctta ttatgatggt ggcagaggat cagtaaaaga tatggtcgca
    19621 ggaggacttg gagcagtcgg agcgcaagga attgctgatc gtttgaacaa tatgggagac
    19681 agcaatgctg atgctaagac aatcgctgat aaagttgttc ctgaaacgcc tgcttcagta
    19741 ccatttaatg gtggcggagt tagtggaaca atcaataata cctctttcaa agatttagtt
    19801 cgtcaagatc aattacgtaa taactcgcaa caatatagcg atcatctagg acggatggtt
    19861 gatgaaaaag gaaactttgt cgatgaaaat ggtgaagtat taaaagacag taaatttggt
    19921 aaagatgcca aaggaaactt tattgatgac aaagggcgtt tggtcgatga agatggaaat
    19981 ttgatagata caaacggtaa accattggaa gacaatgaag atggaaacat cggtatggat
    20041 agccaaggac gtttaattga tgacaaaggg cgcttggtcg atgacgaagg aaaccttgtt
    20101 gatgaaaacg gacgtaaatt acttaacggt gattttgggc aggatgtcaa tggtcgtcta
    20161 gttgatgata aaggacgatt agttgatgaa gacggaaatc tggtcgatga gtttggtaag
    20221 aaacttggaa aacacagtaa cggttataac gtaaatggcg atacagtcga tgaagatgga
    20281 aacatcattg attcaagaga tgctcttgta gcagatcgaa ataaagaact cgaacaacaa
    20341 cagcatacac caattgcggt tgcaatccct gatgatgaaa gcgatgaagt tattttagat
    20401 caagcacatc aagggttaag agaagaagca aaggataaag ttgtttcaga tcaagcacaa
    20461 gaagagctaa aaaataaatc gaatggtgaa gatgtttcaa accaatatca tcaaacggct
    20521 ggaaatgtac ctgttgaagg tcagtctaat gagcttcaca acaactttga taatgttgct
    20581 gatcggaatg atacagtctt aaaagaaaaa gaatcattaa gacaagaaac agataaagac
    20641 caatcaaaat tgaagaaaac aactattcct actcctattg gttcaacagt tccgaaggtc
    20701 aatcaagcta acgattctga aacaaacact gggttaggaa tggcgaaaga caatcaatcc
    20761 attagagaac ctaaaaagac gactgaaagt agcaagtcga acaagtctaa atctacttca
    20821 aataagacag ggaaatctaa ggaaccatct aaagctaaat cagcagtaga atatctatct
    20881 aaaactggta agaacgtgta tacttcagca gataaagctt tagcaaaagc gccaaaagga
    20941 agcttagcag gaatgaccta tgaagctaag aacgaagcta tcaaattagg taaaggaact
    21001 gctaaagttg ccaaagtaac tgctaaagga gcaactaaaa ctggtaaagc agtgaagaaa
    21061 tctacgatgt cggtgaaaga ttatattgca aaatatagcg aacaaaaacc aaccgataaa
    21121 aagtcttaag aagaataaaa agagggagca ttaatatgct ccttcttttt tgttcttctc
    21181 ttcaagggga atgtcaagaa aaacttaatt gtcttcaaaa atttcttttt tatttggtag
    21241 taaatctatg aaatatttgc ttttttagtt atcggagaat aagagggctt atgctagtta
    21301 gttaaaatca ggtaactagt cattaaatgc tgataaatca gcaattttta cgaaaaaaaa
    21361 aagaaaaaaa tttgagggaa aatacctttc aatctacaaa aatttatgtt attatgataa
    21421 cataaagatg taaatatcga agcatctttt ctgtattccg atcattgaca agtgaacaga
    21481 gaaaagtttg cgatgatata attatcttgt gaaaaataat cgaaaggaag gatttataat
    21541 atgtttgatt ctattaagaa tttggtttcc ggaatgacgc cagaaatgaa aacgttggtt
    21601 caagctattc tatatttaat ggctacattt tttgcattga aaccaggatt cactgctatg
    21661 aaacatttcg gtgatgcaaa atggggagaa ggcttaaaat atgttggagc tgttgttgct
    21721 atttttatta tccctgtaat tacaactgtt atgttgttaa cgatgggtac taaaacaggt
    21781 aatgacttaa accaaaaagc taatatgatc tcagctctta ttcctgtggt tggtatttat
    21841 ctagttacaa agtttccaaa agaagaacaa ttaggttaaa aggtaaattg agcaaatttg
    21901 gatgatatgc cgatttgttc aatttacttt ttgctaaaaa actacactcg aagcgtagtt
    21961 tttgaagtga gggaagacaa tagatgtaag atggacagag aaaagatgtt ggttcacgca
    22021 aacgaagtat tgacgagcgg ttttaaaggt accgatttgg cgaagataat gaatatgaac
    22081 gtaaaccagt tctatgatta tcgaaacggc aataaaaaga ttgaaaaagc cagacttgaa
    22141 acgctcatca aatttgaaaa agcgtatcta tacgtgttag acaaacagaa aagaactatt
    22201 gaccgaaaaa aaggagtttt acaataatgg caaaattcaa agatattacc gaatctatta
    22261 attatgtagc agaaacagtt cccgtagttg tactctcttt gaagccacaa ttactcgaag
    22321 tgtaccaaaa agaagaaaaa gaagcgaagg caaaaaaagc taaagcaaag gtatggaaag
    22381 aattcgatgc gttgctgacc aaagatttac caaagaagaa ttacgtggtg attgaggacg
    22441 tacaaaagct cagcagtgag gaatttccgg atgaagaaga tgcgattcta gattccgaaa
    22501 agtacgttgt tttattaaaa tcaagtctga attatgttgt tgatttaccg gaaaaattca
    22561 aagaaaagct attagctggt gtccgcttta tggatagtaa tggtcaatta ttagttattc
    22621 ctgatgatcc taatatgttt gaaagagaag ttgaagaagc tacagacttc tacagtgcag
    22681 tagatcaatt agaggtagct actaaaggct tagaaagaat tgctgagacc ttcaactctt
    22741 ctgatgcggt tgaatttgaa gctgtagcag aagccgaaac agaagccgta ccagatgaag
    22801 aagaagctct ttttgaggat aatgacgaag caactgaagc aggaaaccaa gaagcaagca
    22861 tagaagaaac acaaacagaa acctaccaac aaccaaaaat tgatgaaaaa cccgaatccc
    22921 agagtgataa agaaaattat gtttatgaag ctgaaaaaat cgtatctgaa agtgatgtac
    22981 cgaaaaattt agatgctgat ttgttagaaa aagattttga acagatcata gccaataaat
    23041 ttcaacctgt agttttagat gaggttagtg atcctgttga tttgaacgaa gagatgcgat
    23101 ccattgcaga gttaaaacca ttgctgtttt tagctaaaga gagcatgaac gctcgtatct
    23161 ctattgctaa taataaaatc aacagtgaat taaataaaac gaaagagcaa ttgctactca
    23221 aaggaaagca aatgttagcg gatgagcttt tgcggataga ggaagcaaca tcactaagtg
    23281 gtcaaaatca gtttacaaat acgctatcgg tcgctaaaga aagctacgaa gcagaagcat
    23341 ctaaaattga tgatttagtg atggaaaaat ccagtatcat tcgtaaagat tatgatatgg
    23401 cgaaacaggc ttatttggaa gcagaactaa agaaactaga gcaacggtac gatgctgaac
    23461 atctgccaga agttaaacaa caagaagatg aatttagaaa agcaactgaa gaaagtcttg
    23521 atcaacaata ccaagaagca attgaatcta ttaaaaacga tgctttgaaa caaaatcaag
    23581 aagcaaaaga agttgtggtg gaacggatca tcaattcatt gaaaccggaa attgaaaaat
    23641 ctttaacaga agtgcaagaa aaaacgttag agattgtcga aaaaacaaag gcagaaaatg
    23701 aagctgagtt ccacgataca aaagagaaaa caacagatgt ggttgtcaaa tattttgcga
    23761 acaaagaaga agttgaaaag ctagtagcta aagatacgaa tgagttagaa tccaaacttg
    23821 ttgcacagca aaacattatt gaagattata aaaatcgagt aaaagagttc aaggatttag
    23881 cagatggcta taaattaaat agccaaaaat tgactgaaca aagtgaagct ttacgagaag
    23941 atttatctcg tgcaagaaac gacctatcac aaatggcgga aaaaacaaag tcactagtag
    24001 ttactacaga aaatcaaact gagtctaaac gtgattttac aagcaaaaag atggcgacta
    24061 tcatcgctgt atcagcaatg gcttgtggaa cgattttggg ctcatcctac ttcattagtc
    24121 ataatcacca agaagcccaa gctcaaacag aaacctcaat ttcaagagtg gaaaattcac
    24181 taggatcaaa tacagcttct acttcaacaa cagacaacaa ctatgaagca acaccaacga
    24241 cagaatttaa agtcggagaa gatgttgtca tcagtattga tgggaaaact actaaggctg
    24301 aagtactttc cattgacggt gataaggcag ttgtgaaagc tccagatggg aatcaataca
    24361 aaatacaagc tcacttaact gaaaagaatt aaggagggct aaaatgcgag aagaaaatat
    24421 tgtttatcca gtaattgaaa atcaagcaaa tacaaaatct tctcaatttc ttcaagaagt
    24481 gcaagtcgtt tatccggtta atgaactacc acaaacgaat gaaacagtga gcaagtcaat
    24541 atcattaatt ggcttgctta ttgttgcaac agcgctaact gttggtgttt atcgtaagct
    24601 gaaagtgaaa ggaagtattt ctgatggcag ataactcaaa ctatggattt aaacataatt
    24661 tgcatcaaaa accttctgaa acaatagtca gagttgattg gcttgaaaaa catgtcaaca
    24721 gcacctattc tgttacagca aaaagactaa ttgtttcagc tattgttgtt gcagttgttg
    24781 ttcttggttc aatcgtttat ttggtttccg atacgattaa attgagattt atttttattc
    24841 caacgctatt gatgataatt ggttttctta tactgattag tcagtatatg tttattgatc
    24901 catctagcgg atcactgatc tcaggctcta ttagactgtt taaatcagta gttaacacag
    24961 caagagtaac gatggggaaa aagaaaccat ttcgaggaac ggagattttg aaagttagac
    25021 aagatggaac gattatgtat cgagatggta gtgtagcact aatgtttaat ttggatggtg
    25081 ctacctcgtt aaccgccttc ccagaagaaa tcatagcgca agaaggcaaa tcaattcaat
    25141 accaaaatgc aagggatcgt tccttggctg aaatcaaaat tacttcatca caacctcaga
    25201 atacggaacg tcaaatacgg aacatgaaag tacaaaaaag aaatgcaaag agcaacaaag
    25261 ctgtgtttga catagtagat caacaagaga ggtttgtatc tgaatatatt gatggggtca
    25321 aaactacggt gatccaatat cttattttga agtcacaatc tcagatggta ttggatgaag
    25381 gcgtagaacg tttagaaaat tacacgagta gagggcttta ctatagcgtt actcagttgt
    25441 cagctaaaca aaccaaagaa atattatcag aaatttatgc actgaaataa tagaggtgag
    25501 aaatttaatg gaactaatga gtagatcgaa gctacttaat ttcagaaaaa agaaacacaa
    25561 aaaagagcaa aatgctgata gttctaaagc ggtaaataat acttatgtca gtgatttaac
    25621 atcattggtt ggttatcaat ttacaccatc ttacattagg acaggtaatc ggtggggaac
    25681 gattgttaaa ttagtgaata cgtatggaat gaacagaaat tatgaatttg gatggatggt
    25741 taaagctatt cctgaagtaa aggttgaagg agtaaaagct tacttaatcg aagcggataa
    25801 gccagtttcg caaaaagatc agcaagaaat catgcacgaa aatgtcggtg gatcaacgaa
    25861 cagttatgaa aataacttga acgataaaaa ttcagatgca gaaatcaata aaactgttat
    25921 gcgaatcatt gatttgaata cagcatctct gcttgacagt acggcaaaca aaattattga
    25981 ttggaagtta gaaatcctct tagtatccga ttctcctaac aagatcagcg aacagttgat
    26041 caaactgcaa cgcctgtatg atgaaaaaat gtatggatta aaacttatgt cttttggtgg
    26101 cgatcaacgt gatctgtttg ataggctttt aagtgagcca gtcggtagtg tttacgaata
    26161 cacatctatg agttctgatt ttgcaggaaa tgatcatgct attcgtaaag gcttagatga
    26221 tgcagatgga tgggcgatcg gtacgatgtc tacaagctac gcttcaggtg tagcgtttat
    26281 ggcaatggat cggtctttta agggtaaagt cttagtagca tcccacaaag atgcaacaat
    26341 caaaggctat agggaagaaa tctcagcatc atcactttgg gggcaattga ttgctaataa
    26401 cgcaatggca aatcataaac ggacgtttca catcgtatta aataaatttg actactttgg
    26461 tgatccgaca ggggaaacat ttgttgctaa gccatcttta aatagtgtga tagagaagat
    26521 taatctagct gaagaaggac ttaattcttt agagcttttt ggtgatcgaa aggatattgg
    26581 agatttatat agacgtaatc ttgataaact aactcagatt ttctatctgt tagcggacag
    26641 agaattaaat cctgcaacaa gagtgttgct gaagaaagag ttggggaact tttatattag
    26701 tcagaagctt tgggatatta actatgaaga gcggtcgaaa ctgttagtaa acagcagtga
    26761 tacaatgccg gttcttggag attttatcag ctatttaaca aatgctgaga caaaagcgaa
    26821 aaaaaccggt acagaaaaag aaattgatcg gattattgta ttaaaagata cattgcgaac
    26881 agcattggat agcttctcta agatgttcaa tcagagaact acattgaaag aaattggcaa
    26941 gagcgacaaa ttgcagtttt actacaattt gagcgattta agaacagaaa tcaatgtcat
    27001 ggaagcccaa tttttaaata cctttgactt catcacgtca caagctaaaa aaggcgatat
    27061 cgttatgatc catgggtttg atagattatc agttgaaacc gttggattaa ttaaaaagcg
    27121 cctaacaatg cttgaagaag atggagttcg gcttgcgttt ctctttgata ctatcggatc
    27181 aggagaagat aaagacggta tcgaaacagc tgatattttc aatacagatg gaaaaattta
    27241 tcaaagtttt gaaagtgatt ttaactacac tatcatcggg aaaatgacca gtgaagaatt
    27301 gatgaagtat gaaaagaagg ttgctcctgc aagaaaagga gaagatgatc aaaaactatc
    27361 agtggatttg agacagtatt tgacatcagg tcgaagcaat aaatttcaaa tcagaagacc
    27421 agtcgatttg acgaacaatt ttgttattgg agagtttatt atttaagttc tatccttatg
    27481 gtatcggaaa attgtgtcta gaaggaagaa atatccgtca aaacaaacca tacttttttt
    27541 ggaaaagcgg agttttagaa caatttccta ggagaaatag ataaaacaaa aggggcgaat
    27601 ggttatgaaa caatatttaa atgaatggaa agtgatagag gggtcattag tagcacaaag
    27661 aataaaggga ttacctgatt gcttagagaa ggatcatttg tttcaaatac gagaaatgtt
    27721 aaaaaaagaa cagtttgatc cagaccaatt tttagtagtc gagtatccaa ctattggggt
    27781 gtactgttgt aaccatatca atgatgaaaa atattttatt attcaggaat atgaaggaca
    27841 attaactcct ttctatacta catgggaaat gagtgaggat gggatcaata attttccatg
    27901 tgaaagcatt gaagagtcaa tatctcaaac cgaatgctaa ttgattataa ctataagaaa
    27961 ggtagtgagt taatggatac agctatattg tatattaaca taattccgtc tatatccttt
    28021 atcattactc ttacaatttt catacgtaga aaaaaatttt ttacagagaa gaagcttgta
    28081 tgctttttga tgaaaagtgg agtagtattt cttttttctt ggttattgct tattttatca
    28141 acttcaagtt ttttaattat attatttgcg actgtgggtc ttatagactt tggtgtaata
    28201 tcaggagcag taatgtggtt ttggttaact agaaaaccta aaaaaaggaa caagaaaaat
    28261 ggtttggaaa ataaacgatt atttagatta atgacaaatc attaaagcta taggcatatt
    28321 aagagataat atttaccaat ttcctgattg ttttgaaaac gagcatctgt atcaaatcaa
    28381 aaacatgatg aattgatttt gttatattga aatagatatt aagaaaaagc attccttgtt
    28441 aaaacacaag ggatgctttt tgtcttcact taatctgttt aatataatct aacaactttt
    28501 cttcggaatt aattttttca cctaggtatt gttcagactt attaacaaga ttcaatttaa
    28561 gatcatttaa tatttctgga tacgtatcac ttttcgattc agctaattca tacagttttt
    28621 ttagttcctt atcttttaac tgaaacttct gatccatagt gacagcattt ggatatagaa
    28681 gtccattata atgtgtgcgt aaatccatgt ggaatttttg tttataaaag tctagcagat
    28741 tagaaataaa agaagaaaaa taaccattat agtaaaactg attatttcta tttaagttat
    28801 acaacaattt attaaattga tcctgattta atttgtcgta aatccatggc aatatttcca
    28861 tgtccttatc tgagtatcta aatgcaggag aatcggtcag atgctcaatc ataaaatcta
    28921 agaacatctc tttttcacca aaatatagat attgattata tatcgtttct aaggtagaag
    28981 ttgctagaga agagactctc atgttttgct gaagtattgg atcaagtagt ctatttaaat
    29041 gttcttgtaa ttcttccttg gtgtttccta gaactgcaag tgaacgtaga cggtagtaat
    29101 cttctatatg tgttgttatt ggctgttttg gttcaaaatc ctttattgat atttttaagt
    29161 tttgaattat ttcttctgaa aaatcacgct gtaattctgg atgactagct ataaaggtaa
    29221 gcaaaaagaa gccttttgta taagtaatct gttgattttc tccaagtgat ttattaattt
    29281 cctctctcga ttcagctgtg aacctgtagt ttgagatttc tttttttaga atttttaaat
    29341 aaaattcttt gttatcaaaa atcagggcat ctagtagttt gaaagtactt actctatctc
    29401 tatctacttc ttcattccca taaggtaatt taaaaacaaa cttaaaggct gttttacaaa
    29461 ggttttcttt ggctttctca cttagttttt tcagatagct attttttagt gcagagtgga
    29521 attcttcagg tgtaacagac atagaatgta ttcgacttgc atattgtatg atttcgttcg
    29581 ttactttttt tcctaatatt ggatttttta gaaatacaat ttcataggat ttccgtatca
    29641 catcctgaca agtttccttt gttatttctc ttaattccca agtgtcacca ttgtaagtga
    29701 ctactggatg agctgaataa tttctttgat ccttaatata ttttaaatcc tcatattcgt
    29761 agctatcaat tagctcagtc gaattataag ttttttctag tagctccatt tcccatcttg
    29821 gggagtttgg atcacttttt cttaatttag ctatttcttc taaaattttc ttagaagtat
    29881 catcctcgta tatatttgct aaatcagata atttatcaat taagtctaaa acaagcgcac
    29941 tgtaattgac agctatcgct gaacgataat ttccctgata aaaactactt ctgacttctt
    30001 ctaataaagg aacaattcta ggatttttaa ctttgtttat ttcatcatct aatataaaca
    30061 tataactctc cttttttgtt gatatgacaa gtttaccaca aaaaataaat taaaaaacga
    30121 gacatattta tgttattatg gtatcataat aacataaata tatggaatga gatgatgcaa
    30181 aatgggcttg aaacaatcag tttacatctg gttatatcct gataatccca tgtcaataat
    30241 ggctgatatt tttgtaggta gcattttatt gtgtttactg tatctaattt tcttccacaa
    30301 tttgatcggg tacatgatcc ttgattggaa aggaatgttc gatatagaaa gaaaaccatt
    30361 taataataag caaatttata ccacctttgc aggtggagat ttggaagaaa aagaagttga
    30421 tgtgacacat gaaattggat tagctttatc aggaattcaa gatgctgatg tattaggatc
    30481 aatgataacg gataccggat ataacgccta tgaagtattt tatctcatgt attttatatg
    30541 gcgagatagt caagatatta tcaacggtcg tgctgaaaaa acaatcattt caaatactga
    30601 aatttctttc tgtatgggtg aaggggaaat gattgtatat aaagatggat cagcaaacat
    30661 taataccgcc tttggtgata ttgaaactag tattaaagag cttagggagt atgtacagaa
    30721 tccctacaat tttcaatatt tagcaggaag aataaattta gtttaggagt aagaatatga
    30781 aaaaaattat tgatcagcca acaaatcgct taatagtcga aggcgttatt ttcgtaacaa
    30841 tgtatttcac tgtgggaaat ggtatttcaa atacaatcat tcaaaaagac ccacaatact
    30901 tgttgtttag tctttatgct tggattgtgc agatgctttg ggtgtcttta ttaatgctgt
    30961 tgttttattg gatagtacgg gttattcttt tagtgttaaa caaagcaaaa aagaaaaagg
    31021 ataggtgagt aaaaatgaaa gcaacaaaat taaaacttgt actattttca acagcagtat
    31081 tactaacttt atcagcatgt ggtacatcaa agagcgctga taataccaaa gacacaaaat
    31141 ctagcagtac agtggtgact gaaagcagtt cagcgcagac atctacaact atgtcttcaa
    31201 tgactgagaa caatgctgaa aaatttcaaa agtataagga cgtaaatcaa ggtaaaaagc
    31261 cggaagaagt ttataacgag tttatctctt cagtaaaaaa tgatgaaagt tatgggaatg
    31321 atccattaca aaataaaaat ctgactattg ttctttacaa acctgattgt cctgattgcc
    31381 aaaaagcaga atccaaaatt atgcaagaaa tacaagaagg atttagttat gctccaacat
    31441 taatgcacga tattaccatc ggcaaacctg cttacgaata taatgcagtt gcggtagata
    31501 taacagatgg cgtacctgaa tgggtgcaaa atatccctgg tctggctgat gagaacaacc
    31561 aatatcacac gccaacatta gcagtagtaa caccaaagaa aattgatgga acactatact
    31621 gggatttaat gggaatgaca acaaatacat ccgatgaaat gattgaaagc accagtaact
    31681 actatatgga tttgggagcc tataaatcat acgaacagtc tgaacctgtt atggatgatc
    31741 aatctactat taagtagttt ttagggcgta agccctattt ttttaaaatt atgcgataac
    31801 aaattaatga tttctgaaac aaacgttctt actcaactac tcgaaacaca caagctaatt
    31861 aactagatta taaaggagaa tgcgtaatga aagaaatcag tgcaagtgaa gcccatattt
    31921 ctcaaaagaa tcaggttggt atatataagg tgcttcatgg tgaagggatt tataaaatac
    31981 agttacttac tggttacaat aacggtgcaa atgggaagtg gttaaaggca acaagtattc
    32041 gtgaacttag aagtgaagta gaaaaaatta ggcttggtct cacaagtgaa gaaatagcta
    32101 aactgaaggg aaataattct tttgtaattg aaaaagaaaa ggaaaaacat ctactttatc
    32161 gtgagctgaa aattgaagaa tactatgaaa ttgatttacc tgaaaaatta agaattattg
    32221 atcaacgttc agaagaaaaa tggaaaactg aaatatatcc aagcttaaat gactacaaga
    32281 catcaggaaa gcccatgtat aactacacag gctttttaac ctacacacaa tacaaatatg
    32341 cctttcgcaa attaaaaggc attaaacaag ctgaaattgc cctgtatgag atgaaaaaag
    32401 tggatagatt gaaattgatt caagagttag tgcaaaatac atggaatcaa gcgattgtcg
    32461 agctagaaaa agaaaatggg aaaattttaa ctaataaata tctgatcgta aatgaaattg
    32521 aagaattaat tggtttattt tcatgtgaaa aaggtgaagt cttcttgaag tcatcaccaa
    32581 cctattcaat tttggacagt ccgtatcagt ttatacaggc aaggagatga gttagatgtc
    32641 tagcttgaaa gatgactata tccaagcaaa acaagagtat gaaaaaatac agatgttgaa
    32701 atctgagaaa gtcgaattat ttttaacaca aaaattagca gaggatcata ttgctaactt
    32761 agatgctaaa actttagagg aatatgatcg gcagtttaat cagatacatc acaatatcat
    32821 tttgcttgtt gagcaggcaa agcaaaggtt tgaaaaagcg gaatatgatc tattagatca
    32881 aggaattgta tgcttggata agggattgcg aagatattat ggtgcagagt taaaaaagca
    32941 aaatgtagat agaaatgcac taaaacatga aatcttagaa ttgataatgc aacgaatttg
    33001 aagggataga aagcaaatgt cacaaaacat tagtgctgta aaacaattaa tagaagaaga
    33061 tttagagacc acagttcaga gattagcgga acgaatccat tttgcaaatt atcaaaccat
    33121 ctctaactgg tcaagcagga acagaaatcc ggaagaaatt ccaatgaaat ttttttatcg
    33181 aatcggaaaa gaatatggtg ttgatccgat aaaaattttc gagaagacca tgatttatta
    33241 ctatcatgga acacctaacg aagtgttaga acatcttttg gaagaggcaa aagaaaaatc
    33301 atcaatatta gagctatcgc aaaatgacaa agttattcaa gtgaaatgcg ttgatccaac
    33361 aggaaagatt attgtcggtt ttgatattga aagaaaatcc aaaatagatt ttattactca
    33421 attaaaaaag ataaataatt tagatattct aacgcttaga ccagatcatg aacagttgtt
    33481 tatgagtttt aaaaaacaat tgaaagaaat acaaaaaaga tataacaagg tgtttcctaa
    33541 tgcgtgaaaa gctcaaagaa atcagagttt tttggataat taacaaagaa tttatcatct
    33601 tatatactct tggatttctc agcatgatct tgttttggtg gttatattta aaaaagtaat
    33661 tggagagggt caagaggatt tttatcaaga taaggatgtg gaaactgcat gtggaataat
    33721 agattaaaaa caggattgtt attaatagtt atttcctgcg ctatgatgat tggtatgagg
    33781 atacaaaggg aacaatccta ttttgaagtt tctgctaaca acgtaataga aaaatgctat
    33841 tacggtcaac attattggag tgaggaagtc agagaaaata tagatcgtga atatgttcaa
    33901 agaattgttt gggatgcata ttctatcaaa gattatccta aaagtttaac gagtagatta
    33961 ttttacagcg aaaaagataa tcaaaaactc tcagatttga tgatgaaaaa agtaaggaaa
    34021 cttgcacaaa gctatttgga agaaaaagca ggagtaataa aagataagga ataaatggag
    34081 aaatttgcta ttgaatgaag aaacggaatt atttttaaaa aaagtgaact ttcttgtaga
    34141 aaaatatttt ttaacaaagc acaaaattat gtatctaata aaaggattga tcggtggaat
    34201 tattttatgg atacttactt tcttttcttt atttggtgga tttactaatt ataaaaagtt
    34261 agtagacgaa aatgtgaagc aaattagtgc atctgaatca ttaagacatc aagctgaaga
    34321 tttagtaagt aatccattat ttataggagg tatggtagaa gtgataataa ttagcttcgt
    34381 tattggttat ttaggtatgt gtatcttcca gataatcttt gatctaataa gaggaaaagt
    34441 ctttcaagaa tttaaatctg aaaaaataaa tttacgttag agataatagg ctatgagagg
    34501 agaagaatag tggaacaaac taatcatatt actgaagaaa ctaggaaatt catttgttta
    34561 gaatcgtttt attcggaagg taggtattgt aacaaaggtg aaacttatac ggcatatcct
    34621 attgaagggg gatttaaatt agtttttgaa aacggagaca tgaattttac cactgagttg
    34681 tttgagtgtg ttcttgaaac gtggtcagat gtgttgcttg aagtcactaa ataatcaatg
    34741 ttatctagta gaattaagca actaaaaaag aaagaaggaa gaaaatatgg cgtatgaaaa
    34801 tttacaatta gtagtagcag gagttagtgg cgatatatat cttgcaaaag caaaagatgg
    34861 aattatggac acgaactata gaagagttgc tacaaatgac gttttagtag caacgacaga
    34921 atggttcatt agaaataaca aaaagttaat ccaatttgaa gcagatgaaa atggtagaca
    34981 tagcttgttt tatacaaacg atcaaaaaaa agctgatcgg atcatagcga ttctaaagga
    35041 gaatgatgat ggtggacaaa gagattaagg gcgaaataag attatttcaa agagaaaatg
    35101 acgattgggg tacggaaata tcaaagaatc ttgctgaaga tatagagtta cgattgagct
    35161 ttataagtat gataattgaa agttttgctt ttgagcttgg tataccgcaa gaaaagttag
    35221 cagaaatact gattaaagca agtaacaata gtcctcagca ctttcacatt gatctaggta
    35281 cagtaaatta gtctttaatc attaatatta atttttttga taagctgatc tgattaatat
    35341 ttcttaatgt aatcattcca atattgatct tcagccgtta acggaatata tagaggaaag
    35401 cggtgaaatg aaatgtctga aaatcatttg aaacgattat cgaaaggaac ggcaagtatg
    35461 tttgatgcca atctactaat ctttaaaagc ctgctagaat ttgaaaaaca acgtgtagaa
    35521 gatattgcta agtatggtaa ctatgctact gttccgagtg tttacaatga tttaatgaaa
    35581 tggttcaagg tacaaacagg gttggaatat agccaacaga atttagtggt aaaaatagag
    35641 gagtatgaaa atcatggcta aaagtttatc acaagaatta agagaatttg cttttagaga
    35701 gtgatgatcc tatagctgaa ctagatcaat tagcggaaag agcaaaagaa ttagaaatga
    35761 tttctggtgc gtatagaatt atttctgaag aattaggtca agcggttgtg tcacaagcac
    35821 ttgaaaatta tttgaaaaaa caagttgatt agtgacagaa ataacggaat aggatataaa
    35881 tgatggattc aagatgatat tagttaataa aaaatagtat ggagggcaac taattatgcc
    35941 agtaaaaaca aacactgaat ggttagatat atgtgatgaa ctatttgata ctaaagacaa
    36001 gaaagaactg gcgatgcgtg tagcatatgc tatagttcaa aaagataaat attttgctgt
    36061 tccatctaca gactatgaca ttgaagtgat agttgaccct attactggag ataagaaatt
    36121 acgagtagtt attgagtgta acgcctaatg tattctttag aacaatagga ggaatcttat
    36181 ttcttaaaag tgcaggaatc atatagaaga gtggttgcta accgtcttaa gtatattaga
    36241 aagagatgca aatttaatgg atatttttga agttaaacct gattcaggaa aatttattgt
    36301 tttgcaggaa aaaagagtga ttgcaattga aagaacagaa atagaagcat ataaaaagat
    36361 acatgaacta gcagaacaat atattgaaga tttgaacaat aattttcaag aatttctaac
    36421 gcaaaatgaa taggcaggta tagacagaga taacaaacta ggaaaggaag accataaatg
    36481 aaaatttttg aaattgatga cccgtatttt gcacttatag tagctgaaaa cgaagaaaaa
    36541 tgtatggata tatatgaaga agtaatttgt gatgtagaag gcaaagaaga atttttacct
    36601 tgtataaaag aacttgaaac gacagttgcg attacaaagg ttgcaaatgc agtgagtaat
    36661 gaaacaaacg agctagtagg aattcatgaa gctggtaatc ttgtgtttag ttgtcttaat
    36721 agcaataaat ctgcattatt ggctcttgat ggggatttat tttaatccaa caaccaccaa
    36781 aatcacctaa aatggtcgat ttgaccagtt tggataaata attgtgtaga tcaattttgt
    36841 aaatgaagat gttagataaa ctagacatca atctgtatag ctaaagaaaa gagaacggta
    36901 aattatatcc gctctctttt ctttagctat ttatagaata taccttctct aaatccttcc
    36961 atcaatgtga gcttgcttaa ttcatttgaa attaaaaaag ttattgctat tatacacatc
    37021 gttgtgtata atataaagtg taaatataat tgttcctttg agctattcat tagagttgaa
    37081 gcatagaata gtttcataga atccatagta aaaaatttga gagtaaagga gaagttatga
    37141 gcgaatttga atttttagat aaaagtattg gttctgcttt tttgattgat cctgatctgt
    37201 ttggagcatg ggctcaatca tcaggttata attgggcttt tagtgatgat atggtcgagc
    37261 ttttaagtca aacgatgaat tgggaagctt ataagatcaa gcaattttta gatgttgttg
    37321 cacagccgat ttatacatct gctgatgatc attggtcagg tagagagttg acaacactca
    37381 aagaactagc ggagtacaat aaaagataca aaattccgtt tcaagtttct tacatgtcag
    37441 atgatttttc cgttatttac tcaataggta aagaaagcag atatttaatc cctaataggc
    37501 acttagagat aaagatgaat agcgattata ccgactatac gctaagtgag ttaaaagctc
    37561 ttagacagag cgacagtgag gaatttagcg cactcaaaaa tgaagtgagt gcagataatt
    37621 ttcgcagatc agatattgaa gaactgaaaa aaaagcaaaa acagcaacta gaaaagcaaa
    37681 aaaaattaat tgaagacatt gaatataaac gagtagaaga gcttgcaata caagaagcca
    37741 aaattgaaaa aatgcgacaa gaactctata aaaaacagca gtcaatgtta gcagaagcaa
    37801 ataaaatgcg gtttgaatta gagaaaaaag tagaagattt gaatactaaa attttttatt
    37861 tggaaactga gattaatgca atcgaaggct attttggtga gagtacaact tttgttcagt
    37921 tgcgttcagg tcaaaagagc ttgccggaag aaccagtggt gatgtttcaa aaagtacgat
    37981 atctagatga agaattagga agatatttca gtatgcgaga agttgaaggt acagctgata
    38041 ccatgctgac ttttgagcag gcgctatcct caagagatga tttgtttgat ttattctcac
    38101 cgaacaaaaa gtcaatctca atgatacgta tttccaaatc aggatatggc tacactaacg
    38161 gtcaaatgga aagtacaaac atgctagaaa agtataaaaa atatcatgga gaaactttag
    38221 caattttact gagaaacggt gataacttat ggatttgttg gttggatgaa aataagatca
    38281 gtttcaaaga tgatttgttt taccaaaaca aagtaacaga gcaggcatct gaaaccaaaa
    38341 atatggtaaa ggagatagat tggacacata ctcgtaattt agaagagcga ttgaaagatg
    38401 ctaaaaagca gaagaaggct gttagaggag agtttgctag tcgttatatt cttttttcaa
    38461 ttttgcaagg tgttctagat aatggaacga tgatgacctt accagaaaaa gtaaatgtga
    38521 tccaagcaat taatcagaaa agcccatatt ttattgcaag cgtagcggat ggatggttag
    38581 aagacacacg cttgccgtca tttagcgagt tgattgataa atacagtttc tataaagaaa
    38641 caggtgaaga cagagtatta gaagcggaat atgagttgaa tcccgataaa tttgaaaata
    38701 tgagagatta tccacacaaa caaaatgatg acatttatgt gttataccgg ttacgtgggt
    38761 ggtcgcctac atcagcaaat tacacttatt acaacagtga taaccgttca aatagtgaga
    38821 gtggacgtga cttagcatat tcgattgact tgaaagacgg catttatcag atcaatttag
    38881 taaaacaggg aaaattccaa aaacaaatgt tttcccgaac gtttgttgga gttgattcta
    38941 ctacaggcga aaatgtctat gattactatt atgaaagaga aacagagtac tatattcgag
    39001 gacaaaaagc ctacaacaaa agtgactatt ggggtgacaa ggcgaagcga ttaactgcaa
    39061 attttagagt gtatgaatcc gaatttataa acttaacctt tcttaatagt gtttttattg
    39121 attatgtcat tactacgaaa cgtattggaa gttattcaag tacctacgca tccttagttc
    39181 caatgttaaa ctcgatgagc cgtttcttaa aagaaagaga agagaaagag ctagagtata
    39241 tcaaagaaat tatgccagat tttgaaatgc acgaatggca aattgcttta tcagattgga
    39301 aattgacgaa taaggttcgt agaataacac cttttcaagc caagcgcttt ttgaaagcta
    39361 tgaaatttgt tcaatagaaa aatccaccag acaagctgat ggagactaac ccaattacta
    39421 acagttgtaa ttggtgttag cttataaatt aattatggtg tttagtcaat cccaaattgt
    39481 gaaacggcac caataattgg aaaggaaaaa tgaaatgtct gaagatttac gagagctgaa
    39541 gttacgaaag aagcagtatg accagtattt ttccggtttg ttgaataaac atattgaaga
    39601 aggacttgat catgattttg gaaaagatga atattgtttg tattgcgagc gttacaatga
    39661 aaaaatgtct gaatatctac agctgaagaa agagatacag aaattagaga aaaaagtaca
    39721 tgcttggaag tataaagatt ccatgtccgg ttatggtgaa tctcaaaata agccaagaaa
    39781 aaagaagcaa gcgtatgttc taaagatggt aaatggggaa gagttaattg tattagggta
    39841 cagtaaagaa gaggttcgag aactcgttga aacacctgtc aggaaaaagg tagaaacaat
    39901 aaatgagata gacgacagcc aatacaatag catctatatt gaaacaagtc atggtggata
    39961 taagaaatta gcaactttcc ttagaagttc taaagttggg ataatttcta aaaaagacat
    40021 aagtgctgtt accgatagtt tttgtacttt atctgttcct aaagcatatt ttcaatacaa
    40081 cagtaataga tttgatcgct tggagaaatt ctgtagacca tatactgtat tgaaaactaa
    40141 aaaattcaat aaaactaaaa gagtttcaat caagttaccg actggtcaaa tctcgctagg
    40201 cgaaaaagat tatctgataa aagtaggaaa agagttaata atcaaagtgc cggaacgctt
    40261 atttaaagaa aattttgaga tacgtgaaac cttggtatgg aaagaagaat aatagtttgg
    40321 aagacatagc agggtataag gaggaattga aatggcagta aatagaaaat tgcaggtatt
    40381 gattacagac gactttggcg gagcagagtg gtttgatgtg cagtctatag attttgaaga
    40441 agaagtcgta gttatagaaa atgtatacgg cgaagaatac tcatattcgc ttgaaggaga
    40501 gaatgttgag gatttacggt tgattggtga gtagaaaata gcagaaatat caaaatggag
    40561 atgaaatcag cgcgtatatg attaacaaga aaacaggaaa acgtattttc agcagtaaag
    40621 acgaggtaat tcgggatttg agtgaaaact ttgaaccaat tgaattattt ttccatcagt
    40681 taaatctaga aattgaaaaa ttactaaagg aacctacaga aggacgaatt aataaagtat
    40741 tgaaattaat agcatgtaaa gatagtcatg cttacatgct ttatccaaaa tggtatgcag
    40801 acaaaaatat agctgaaata aacgattatc tagctagtat aaaggaacgc aatgcaatta
    40861 attaacaata gacgaaaaat gaaattaaag gagataggaa aatggcagaa gttaaatttg
    40921 gatcaaaaga atggtttgaa gagttaaact caatgattca gtttccagaa aagttcactt
    40981 tttactacaa caaaagagac aatatcattc aagtagcttt acttgaaact aagggaagat
    41041 tagaaaatac tcgatggagt atgataaaac catcagcact cagatttcat cctaaagact
    41101 tgacagcggt tgtagcggta aaagaggttc aagactcaca agaatactat gaagtagtaa
    41161 aaaatctgaa ggatatctgg tgtaaataat tctaaattga ccagttattt ggtcaaaaat
    41221 agtatgtgat tatttcatcc aatatggcat cataattggt atgaaagggg ataaattatt
    41281 tgaaagaact agaaggtatt aagaaaagaa atcaggaaag attggaactg ttaaatgaaa
    41341 atagaaatag gatgtttagt tactatgaat ccatgaggtg tttaaataaa tctagaagtg
    41401 aaattataga atcatcaaag tataaacaat tgatggaaga gagagtttct ctcattcaag
    41461 aaatagaaac agagtctatt tttccagatc attttcctaa agtccgattt tataattaaa
    41521 cattaacaaa tgaaacgatg ctaaataggc atcgtttttt atttatttgt taaatttatt
    41581 tatttacaaa atgtgtatca gtgtgtataa tatatttgta gataagttaa gaaaggatga
    41641 tctgaaatga aattagaagg tagcttacaa aacagaatga tggaaggcag aacaacaaat
    41701 aaggagattg tagttggtat gggagcaacc gagcttctat ataccgacag aaatccttat
    41761 acagtgattg aagtaaaaag caaaaacaga attttggttc aagcggatgg ggcaatcaat
    41821 aaagctactt ttccagatca agagtgggaa tacagcagaa atcctgaagg tcaaatattg
    41881 gaattaatta agacaaaaaa tggatggaaa gtgcttaaag aagatactta tttttatata
    41941 ggtgatagag aaaaatacta cgatcctagt ttataaacaa attgaagatg agcgaggttt
    42001 tatcctcgct cagaaaggag aatgaaggat gttcagtcaa gaagaaatca tcagcaaatc
    42061 gcaaccggat aaaaatggaa ctataaccat gaagaaaaaa attccttgct atcgttgtag
    42121 cggatcaggt cagtacaaat ttatgtctat ggggaaaatt acctatggca catgctttaa
    42181 gtgcgatggt gacggatttc ttgttgtaac tgaaaaatca tttacgccag aatatagagc
    42241 taagctaaat gctttaaata aaaaaagggc tgatcgaaaa ataaggatca caagagagaa
    42301 agaatctcag aaaaattttg atcgttatgg ttttgaaaag ggttatattt acgtggttat
    42361 agaggaaaat agctatgcga tcaaagatga actgcatcat caaggagcta cttatgatcc
    42421 tttattaggt tggtttttca aagaaaaaca agaacaatac aaaactcaaa aagtttttgt
    42481 agaagaattt tccgtaatca atgatgaagg aattattgaa ttgaagctag aagaatacag
    42541 cgaaagacaa caggagattt taagggcgaa gcgtagagaa gagggtgagt ttattggtac
    42601 aatcgctcaa cgattggaat tggatttgaa gcttaccaat accgtcacca tagatacaca
    42661 atggggcaat gtttatctgc atttatttga agatcaaaac cataattcat tgatttggaa
    42721 gacaaataaa tttctttatt atgatgaatg ggatgttgga gaaataagga aagttaaagc
    42781 gacaataaaa gcgcatgatt actacagaga taccaagcag acttgggtta aaaatgttaa
    42841 aattcaaaaa aatgagtcag aagggataaa agtatgaaaa gagaggaata caaacaaaga
    42901 ttaaacgaac tgcttgaaga ggacgaaaca ttaactcatg gttcgcctga tgaaattcta
    42961 tacatgattg ataatatggt gatctttggt ggatatgaac ttggtaatag gtcagttgat
    43021 cataatattt tagaatttga tgatgtatca tgggaagaaa ttcttgattg gggaattttg
    43081 gcggtacctg aaactaaaac ctatattagt gacactatgg ttcctttttt tgaggaattg
    43141 gattataaaa gacttcccaa aaatgaaaat catatactgg gaggaaacta acaatgttaa
    43201 gtgacttatt tattttactg atctatctag gtgtaagtga aattattgcg tattttttgt
    43261 ttgtcattgc taattatggt atttccagtg caatctttca aaggtttaga ttaccagtaa
    43321 gtgaactaaa aaaaatgatg agattatcag catatctttt aatgtttttg aatgtggtca
    43381 cgttaatctt tgtagtaatc ggtgtgactt tcaatttaat ggaatagagg gaaatctata
    43441 atggaaaaaa gagaaaagaa tgaacatatt tttttgaatc aatattgttg atggtttttg
    43501 cgatcagcgc ttaccagaaa tctaaaaaaa gagtaatgat tgtttaatca tcactctttt
    43561 tttgtaggga aattgaaata tgtggagctt gtattggttt agttaggatt gcttggctat
    43621 aaggagctag ttctttatca aaaccatcaa agtaggtttt cagtagcgat ttaactgatc
    43681 gctcatcttg tttgatttgg ataatttcag catccaaatc ggttgtgaga tcaggagctt
    43741 ttgatgcgat tacctgattt tttttatctt ccaactctct taggctgttt aacaagttgt
    43801 aaaaagaatc attttgattg ttcattccaa cgtgagttgc aattttaaca gaagtttcgt
    43861 ccgtattgtc atcaaaagaa ctcattggaa tatcaagtag taacgaggat gtttctttta
    43921 ctgacttttt ctttacagca ttttttgttt ttccatcttt ttttctcttt actcgagatg
    43981 tttttggagc tagtttaggt aaatcacttg ttttatcagt catttattag cttttccttt
    44041 ctattaattt atgttattat attatcataa taacataaaa aagtacactt tttgggggcg
    44101 tagttattga aaaaatctaa gagaaagatt gccacgttgc ttatgttaat ggtgcaattt
    44161 acagggattg caatgagtgt aatcacaata aatccgcaag tgatacatgc tgatgggata
    44221 gctacttatg atgcacaatt taactgtact actgaaggta gtgggtcagg aacagatagc
    44281 gatggaaacg atactggaag tggcgctgta tcaggtgatg tagcaaaaaa tattgatgat
    44341 atttacaaat ttatgaatgg ggaatatggg ctatcaggtg ctactatcgc aggtattcta
    44401 ggtaactgga aacaagaatc aggtaatgat cctcgaacgg tagaaggtta ttacggtgat
    44461 ggacagagag aagttgaatt tatcaaacaa ttcactgatg gtctgatggg tattggctat
    44521 ggtcaatgga cagcaggtcg ccatactgca ttagtaaact gggcgaagaa aaactttggt
    44581 ggcgcttggt ggacgacaga agcgcaattg gattttatgt tcaaaggtga tggtggattt
    44641 gttgctatat taaaagatta tgctttaaac gcttctgatg atgttgtggc aaatgccgta
    44701 gcgtttcata agaattggga aatttcagca gattccgaat cagctgtagt ggctgtacgt
    44761 ggtggaaatg caaaagaaat ttgggaatac atgaaaagca aaggcatgga cggagctaaa
    44821 gatgtaagca aaatcaataa aattgccgga ggaacaagta ccggtggtgg atcaaataat
    44881 tcatcaagct cagcatcagg aacagtagaa gatgcctgtg caactacaga tgatgctaat
    44941 tctggttctg gatcaatggg taaagaagga aaaattaacg gtaaaaaagg tcaagtaata
    45001 gcgaatatga cttatgaaga agctttgaaa aaatatggga aagcgatcac gctaccaaaa
    45061 ttcaaagaac cagattggtc aacttcacct tttaacggaa tatatcaagg tcagtgtaca
    45121 gagttgacgt gggcttatat gagccaatta tacgatggga atcaaccgac tactggaaat
    45181 ggtggctacg tatgggaaag ctacaaatcg gcaggagcga aaataacgaa caaaccaatc
    45241 gtaggttatg ggtttagtgc ttcagatgga tggttatggg ctgttggcga tgcagggcat
    45301 actggtgtag ttatagcggt gtttgaagat gggtcatttc tatgtgctaa ctttaatgtt
    45361 ccaccaaccc aagctccaac aaggggagtt tcggtaactt tgattgatgg agtggacgga
    45421 tcagataatg tgcatttctt tagcggtgta ggaaaagcta aatttgaaat cgaaaaataa
    45481 attggaatga aggacgatat agatgaatag taaagtgaaa gatacaatta tcatttcttt
    45541 gctttctggt atgttattag cactgcttgt tggaatccta ttcttcaata agcagaatca
    45601 ggacatagaa gctgaaaaac acagtgtgca tattgaagat aagctgacat tctttaaaga
    45661 ggttaaacca aatgaggtgg atggattacg gaaagattta gaaaatgata ttcaagaaat
    45721 gttgaatgga tcagttcaaa ataagtttgc agatcaatca ggtgaagaat ttttgagaaa
    45781 actatttatt gtcacaggta aaccaccaat tactgataag agtagtgata aagaaaaaat
    45841 cgagtactat aagcctttta cttttaagct aacaaacttt ggaattaaga catctagtag
    45901 tgggttgtat gaagcattga ccgattttga agttcaatac aatggaaaaa aagtgtatga
    45961 taccacatta accatcacac taaataatga taagaaagtg actgggggtg ctttttatgc
    46021 aaaatcatga aatcactact gctaaaaagg cgattggaat actttccata attgtgatcc
    46081 tttgttgtgt ggtactattt caaaagaaaa atgcttatga taataatgtc aaagaattgg
    46141 aagaccttaa aagtaaacaa atcgaagtag tcaagaataa ccagaaagtg aaagctatgc
    46201 aggaagccca atacaaagaa ttggggaatg ctgaaatcaa ggaacaaagt aggaaattct
    46261 ttaatttatt ttacaactgg aattcatggg aaaaatatac gaagaatatg aaggaaatac
    46321 aaaaagaatt tcctcagata gtaaagtcta gtcctgttga tatttctggc acgatggtag
    46381 gcactggtaa tagtccaata tctagctata ctgctgatta ctatacaaca aatcaaaaag
    46441 gtaaaatggt tgaattgatt acacagacaa gaaatactcc aacaacagca acaacaacaa
    46501 tttggcgtgg agtaacaagc ataggagaaa atcaattgtt ttacttggag aatttagata
    46561 gctatgtaag agcggagtaa aataaaaaaa gagctaacgg taccaacgtt agctctttgt
    46621 gctgacgatg ataacaaaac ctaccgaaaa aggattgttt catcacaaac acaaacagaa
    46681 aagaattgat aaaaaacccg aacctcagaa tttttattaa tcaattcatt tagattataa
    46741 taaattgagc tgaacattac aatacgtata tgttgtaaaa cgtaaaaaaa gtcaaaagta
    46801 aatactttct taaaaagtca aataaaacta ttaaagacaa tatatatggt ttatttgaca
    46861 taagaaaatg gtctagatga taattgtaat atagaattgt aggcttaaat aaagcataaa
    46921 atacctttta tgataaggta tagctcttaa ttttaagcag tttttagata taagagtgaa
    46981 agtaaagtgg gggagaataa taatggcaga tcgtaaatta gaaaagttat tagaagaaac
    47041 gtggaatcca aaagaattta gtgaattttt tatggagaat tttgaaactg acttagcagt
    47101 gattgttaaa gatgctctta gagagcaagg gtatcctgaa actgcaaact atataaacat
    47161 taattttact ttgtatactg aaaataaagg aacttgggat ttttgggcta ctttagcaaa
    47221 taaggagtta agtgataaat cagacacagg aattcgtaac ttttttgaat caaacagaga
    47281 tgattatatg tatgctaacc atcaagataa attgaacttt agagtagagt ttgatgaaac
    47341 acctgaagaa tttatagaaa gacaaccgcc taaagaaaat gtagcaaagg tattggaaga
    47401 tcgttggaat tcagatgaaa tagtttcaac aattagcgaa ctaggcggac aatatgaacc
    47461 gctagtagaa gcggttagag aagagctaag actgaataag tttcctgatg ttcaaaatat
    47521 tgatgtttct caaattgaaa taaacgtcaa gatcacgaat aaacttgatt atggtagttg
    47581 ggcagatata gcattagaaa aatacatcta ctcaacgtta aaagagttta tcgaaaatag
    47641 aatggatatc atgtatctac agcatccaca gtatttaaac tttggtgttg aaatagctac
    47701 acctcttgaa gaatggaaaa tggagcaagg gcttgactag cctttgctcc atttttttat
    47761 tttacaaatg aatacagttc gtcgttttta ccaagaatga ttgtttgccc aactttgaga
    47821 ttgtcaggtg atccttcagc ggtttgtaag gagaacgttc cgttgaactt atcggatacc
    47881 gttataggta gctttaattc tgatccttcc tcatctttga gaattgctgt ttttcgttca
    47941 tcaaaataaa cagtctttat taaataattt ttcgtagtga tctcagcaga tttgtctttt
    48001 tttgtagtag taacagtttc tttagaatta gaggtatttt ctacgactat tccatctttg
    48061 gcatttgagc ttgtgtttat ttgattgaat attttcagcg acaagatacc gctaagaata
    48121 gtcaatatag aaacaagtgt aattaatact gataatttcc tttttttgtt cattttttct
    48181 taacttcctt tttgttttga ttagttagat attccttatc taacaagcct ttttaggcta
    48241 ttaaatgagg ataaacccca aaaaaatatt gcaattatga cagtgcaatg gtactattat
    48301 tatacaataa attttgcgaa aggggtaggt ctaaatcgca aacaaaaaga aattgaacct
    48361 agacaaatac aacttgtaca ccgcaaggga agcaagtttg attttgggta aaggtagaaa
    48421 ttatgttaca aacataatca actattatcc aaatcgtttg cctaaaggtt cgttcaagaa
    48481 agtgggtgac aaaacctata ttataacgga agaaggaata gaagcattgc gatcacaaac
    48541 agaaaataaa aacaaaaaga aggaatgaat atgaagaaaa attcgcttgg tatggtttta
    48601 tttgcatcag ctgtacttgc ggggttatca gtaccagtaa cctcacatgc tgatgaatgg
    48661 actgcaaaca caccggatca aatccaaatt acaacaggac aatcaagtta tgcgttcaaa
    48721 aaaggagata ctatttggtc aactgtagag aagcttaaaa gcttaggtta caagactact
    48781 gttgctgagt ttgccgaatt gaacaatgtt gatttggcaa aaggagaaca acggactatc
    48841 ccaattggac gaactttcaa catcagtaaa gatacattaa cagttaagga agcagatggc
    48901 gaagtagtta gtcaaactgt tcaaaaagat aaacctgatc ctgttgaaaa tacggagcaa
    48961 gagaacaaaa aatccgagac tgcaaaagaa tcggaaccaa cagcaaacac ttctgataag
    49021 acagaaacaa gtgcttcaag tgttgatgga gcaagttcat ctaatacaaa taattctaca
    49081 agtgagaaag aagcatcaca gggaacaagt gaaagtagca ctacggaaac tgatactgga
    49141 acatcaacaa ataactcaca atcaacatca aatgatgcaa atgaatcaac cagtacatct
    49201 tctagtgatc aatcacaaac aggttcgagt gatgaaactg acagttctac ttcaccaagt
    49261 aaacccgaac aaccaactaa tgttgtgcta ggcgttccat ttattagtca aggtaacaca
    49321 atgctttgcg aaggaacatc tttgctagaa gcgttacatt ataaaggtgt tgcaacaaat
    49381 caagatttaa tgacttttgt taaatcaatg ccactatctc ctgataataa tccaaataat
    49441 ggtttttctg gtgaatggag acacaatgtg gatggaactt atcaaggtat gaatccggct
    49501 cctgtagttt catggggtgt tgctaatggc ggaaatgttg tagatatttc tggttcatcg
    49561 gtacaaggat taaaagatga gattgtaaat ggaaatcctg tagtagcgtg ggtaacttat
    49621 gcctttgaac ctgctcaaca taaatcaatg ttttggggcg atgctatttg gaatcgacat
    49681 gtagtagccg ttgatggatt taacagaatt aggcagaagt cgcccatcct ctataggtgg
    49741 gtgatgaatg ccgttcggta tgagggttac cgttggtaac tcgaagaccg acaactaagg
    49801 cgtattgcct ttttattttg t
//
LOCUS       DAHHPA010000009        45347 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.009, whole genome shotgun sequence.
ACCESSION   DAHHPA010000009 DAHHPA010000000
VERSION     DAHHPA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.009"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(141..1628)
                     /locus_tag="NBH55_000486"
     CDS             complement(141..1628)
                     /locus_tag="NBH55_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508501.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
     gene            complement(1951..2430)
                     /gene="rlmH"
                     /locus_tag="NBH55_000487"
     CDS             complement(1951..2430)
                     /gene="rlmH"
                     /locus_tag="NBH55_000487"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCD1508502.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2858..4147
                     /locus_tag="NBH55_000488"
     CDS             2858..4147
                     /locus_tag="NBH55_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCD1508503.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4186..4791)
                     /locus_tag="NBH55_000489"
     CDS             complement(4186..4791)
                     /locus_tag="NBH55_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCD1508504.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4996..5460)
                     /locus_tag="NBH55_000490"
     CDS             complement(4996..5460)
                     /locus_tag="NBH55_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD1508505.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5482..5802)
                     /locus_tag="NBH55_000491"
     CDS             complement(5482..5802)
                     /locus_tag="NBH55_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1508506.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5946..7025)
                     /gene="pepA"
                     /locus_tag="NBH55_000492"
     CDS             complement(5946..7025)
                     /gene="pepA"
                     /locus_tag="NBH55_000492"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCD1508507.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7225..7575
                     /locus_tag="NBH55_000493"
     CDS             7225..7575
                     /locus_tag="NBH55_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD1508508.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7798..8745
                     /locus_tag="NBH55_000494"
     CDS             7798..8745
                     /locus_tag="NBH55_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1508509.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8802..10220)
                     /gene="pepV"
                     /locus_tag="NBH55_000495"
     CDS             complement(8802..10220)
                     /gene="pepV"
                     /locus_tag="NBH55_000495"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCD1508510.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10369..11214
                     /locus_tag="NBH55_000496"
     CDS             10369..11214
                     /locus_tag="NBH55_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCD1508511.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11218..11658
                     /locus_tag="NBH55_000497"
     CDS             11218..11658
                     /locus_tag="NBH55_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD1508512.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11708..11902
                     /locus_tag="NBH55_000498"
     CDS             11708..11902
                     /locus_tag="NBH55_000498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508513.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12038..12724
                     /locus_tag="NBH55_000499"
     CDS             12038..12724
                     /locus_tag="NBH55_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1508514.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13132..13821)
                     /locus_tag="NBH55_000500"
     CDS             complement(13132..13821)
                     /locus_tag="NBH55_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508515.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13796..15925)
                     /locus_tag="NBH55_000501"
     CDS             complement(13796..15925)
                     /locus_tag="NBH55_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCD1508516.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15973..17310)
                     /locus_tag="NBH55_000502"
     CDS             complement(15973..17310)
                     /locus_tag="NBH55_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCD1508517.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17562..17921)
                     /gene="rplT"
                     /locus_tag="NBH55_000503"
     CDS             complement(17562..17921)
                     /gene="rplT"
                     /locus_tag="NBH55_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCD1508518.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17974..18174)
                     /gene="rpmI"
                     /locus_tag="NBH55_000504"
     CDS             complement(17974..18174)
                     /gene="rpmI"
                     /locus_tag="NBH55_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCD1508519.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18246..18770)
                     /gene="infC"
                     /locus_tag="NBH55_000505"
     CDS             complement(18246..18770)
                     /gene="infC"
                     /locus_tag="NBH55_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCD1508520.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18814..18939)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19090..19722
                     /locus_tag="NBH55_000506"
     CDS             19090..19722
                     /locus_tag="NBH55_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCD1508521.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19724..20629)
                     /locus_tag="NBH55_000507"
     CDS             complement(19724..20629)
                     /locus_tag="NBH55_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCD1508522.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20714..21373)
                     /locus_tag="NBH55_000508"
     CDS             complement(20714..21373)
                     /locus_tag="NBH55_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD1508523.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21689..22633
                     /gene="mvk"
                     /locus_tag="NBH55_000509"
     CDS             21689..22633
                     /gene="mvk"
                     /locus_tag="NBH55_000509"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCD1508524.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22626..23603
                     /gene="mvaD"
                     /locus_tag="NBH55_000510"
     CDS             22626..23603
                     /gene="mvaD"
                     /locus_tag="NBH55_000510"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCD1508525.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23616..24701
                     /locus_tag="NBH55_000511"
     CDS             23616..24701
                     /locus_tag="NBH55_000511"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCD1508526.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24698..25762
                     /locus_tag="NBH55_000512"
     CDS             24698..25762
                     /locus_tag="NBH55_000512"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCD1508527.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26011..26814)
                     /locus_tag="NBH55_000513"
     CDS             complement(26011..26814)
                     /locus_tag="NBH55_000513"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCD1508528.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26834..27676)
                     /gene="kduI"
                     /locus_tag="NBH55_000514"
     CDS             complement(26834..27676)
                     /gene="kduI"
                     /locus_tag="NBH55_000514"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCD1508529.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27692..28699)
                     /locus_tag="NBH55_000515"
     CDS             complement(27692..28699)
                     /locus_tag="NBH55_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCD1508530.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28706..29350)
                     /locus_tag="NBH55_000516"
     CDS             complement(28706..29350)
                     /locus_tag="NBH55_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCD1508531.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29450..30208)
                     /locus_tag="NBH55_000517"
     CDS             complement(29450..30208)
                     /locus_tag="NBH55_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCD1508532.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30335..31486)
                     /gene="fucO"
                     /locus_tag="NBH55_000518"
     CDS             complement(30335..31486)
                     /gene="fucO"
                     /locus_tag="NBH55_000518"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCD1508533.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31568..31879)
                     /gene="rhaM"
                     /locus_tag="NBH55_000519"
     CDS             complement(31568..31879)
                     /gene="rhaM"
                     /locus_tag="NBH55_000519"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCD1508534.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31881..32726)
                     /gene="rhaD"
                     /locus_tag="NBH55_000520"
     CDS             complement(31881..32726)
                     /gene="rhaD"
                     /locus_tag="NBH55_000520"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCD1508535.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32740..33996)
                     /gene="rhaA"
                     /locus_tag="NBH55_000521"
     CDS             complement(32740..33996)
                     /gene="rhaA"
                     /locus_tag="NBH55_000521"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCD1508536.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33993..35453)
                     /gene="rhaB"
                     /locus_tag="NBH55_000522"
     CDS             complement(33993..35453)
                     /gene="rhaB"
                     /locus_tag="NBH55_000522"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCD1508537.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35606..36475
                     /locus_tag="NBH55_000523"
     CDS             35606..36475
                     /locus_tag="NBH55_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCD1508538.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36601..37500)
                     /locus_tag="NBH55_000524"
     CDS             complement(36601..37500)
                     /locus_tag="NBH55_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508539.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37665..38489)
                     /locus_tag="NBH55_000525"
     CDS             complement(37665..38489)
                     /locus_tag="NBH55_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD1508540.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38500..39795)
                     /locus_tag="NBH55_000526"
     CDS             complement(38500..39795)
                     /locus_tag="NBH55_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCD1508541.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39811..40290)
                     /locus_tag="NBH55_000527"
     CDS             complement(39811..40290)
                     /locus_tag="NBH55_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCD1508542.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40296..41324)
                     /locus_tag="NBH55_000528"
     CDS             complement(40296..41324)
                     /locus_tag="NBH55_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCD1508543.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41341..42447)
                     /locus_tag="NBH55_000529"
     CDS             complement(41341..42447)
                     /locus_tag="NBH55_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCD1508544.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42676..45273)
                     /gene="adhE"
                     /locus_tag="NBH55_000530"
                     /gene_synonym="adhC"
     CDS             complement(42676..45273)
                     /gene="adhE"
                     /locus_tag="NBH55_000530"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCD1508545.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        13119 a         9660 c         8238 g        14330 t
ORIGIN      
        1 ttatttttat atgttgtaac ttcctgataa gaaaactaat taaaagaaag aggtaactac
       61 ataaactaat taaaaaagct aacttcatag ttggaggata aatggaagaa acttcaatga
      121 ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc tcttttttgt
      181 ccatttactt ttgcgactgt tccccaataa tcaaatacag gaagcgttat tttttgtcct
      241 ttagattcat gtactgtata gactattttc gaatccatca cttgataagg catagtatgc
      301 cattccttgc caattcttac ttcatgattc tgcaaatgtt ctttgtattg taatgcttcc
      361 gtgggtgcat agtcataaag tcctccggga aggagtgtcg tgtaacgaat aagattttct
      421 tgattcatta ccaagtgatc tctctgagtg acaactttat gttcatagtt tgcttttgtt
      481 gcagcagata cagccaacag taataaaaca attgccgtta tattaactac ttttctttgc
      541 ttagctgatg tgctcttcat gaaaacgaac gataaaatca acgagccaaa cagtacttga
      601 aagcctaaga ttcgccaagg aaattgaatc atttgaatgg gtgtattttg taataaagac
      661 catggaaaaa atttggtgga taagagccaa gagactaccg cactaaggta aatcactttt
      721 tctttgcgat taaatccttt aaaaaagaag agtggacaga tcaaagaaat aataccaatg
      781 attcctaccg taaaggttgt caggtcatta ttcatcgcac caatcaaact atccgagaca
      841 tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat taatcgattg aaccatgatt
      901 tgtcggaaca tcggatacca aaagtatgct gttaaaagaa tcgtcattcc tactgccttt
      961 gacaactcca caatcaaccg gatgctttct ttttttcttg tttcacttac tacaaagccg
     1021 cctaagacta gtaagccaac ggctattaca ctcataaaag tggaaagaac atgtgtataa
     1081 atgagtaagc tcatactgac tgctaatgga attgcacatg gctttttctc ataaatcagt
     1141 gcgtaagcat aatacataac tatcggtata accgtaagtg caatcgcttc agcaatagca
     1201 gaacgatgat aaatatcaat cgtacggtac ccataaaatg tatataaaca gctaaatata
     1261 aaagccgcat cgattctttt taaaaatttt agtccataat gaaacatcag catgatagta
     1321 caaacattca atagccaaac atataagata taactaacga ttaaattatt acttatccaa
     1381 taaaaaatga ctgccggaaa aaaggtcaag tagggataaa aataattcac tccgctccca
     1441 taatgattaa acgtcgtaaa attgattgga ccagaaaaaa ttgaagataa ccctttgact
     1501 ctagcaaaat ggaaggcaat atcccccgat gtagaaatag tatgaacatt tctaaaataa
     1561 ttgattgaat aaagtccagc aataattgct aggagaatgg acaatattat ttttttcttt
     1621 acttgcatta cttaattata tactcctttc atggattgct ttatctagta taacttaaat
     1681 tttaaataaa tcgttattta tttttactct cacttaaaaa agcactaaaa ttataagaga
     1741 gggaggtaat ggtctgaaca ccaagaattg ttccaacatc gaattgctta actaatgagt
     1801 tcaaagctta attatgtggc tcttatattt accgagcgtg gattgcgatc tctttagcga
     1861 aacaaacatc tttcataaaa aaaacagcca tatccgcttt cttggatatg actatttact
     1921 actactttac tatctttcat tcttcttgtc tcacttatga taaggcgccc ccgcattgat
     1981 ccggaaggca cggtagattt gctcgacaag gatcagacgc atcaattgat gtggatacgt
     2041 catttttcca aaagagatct gagcattgct tcttttcatg acggcttcgc tgagtcctaa
     2101 agagccgccg ataacaaaaa caaggtggct ttttccttgg atacctaatt ggtcgatttg
     2161 tttagcaaaa gattcacttg taggattttt cccttcaatt gctaaggcat agacgtattc
     2221 ttgttctttg attttcgcta agatccgttc cccttctttt tcttttactt gtctcatttg
     2281 cgcttcgctt aaattctccg gggctttttc gtcaggaact tcgatcaatt cgatttttgc
     2341 ataggcattc agccgtttga catactcatt gataccttgg atcaggtatt tttcttttaa
     2401 ttttcctact gagataattt tgatattcat gttttctctc ctttggtctt catgctcact
     2461 ttgtttatta taatcaattt tttcgtctat atgtttattt tggctatttt tgttaaaaag
     2521 atttccccaa cagttatgca caacatccac aggtttatcc acagaaaaca gtgttatttt
     2581 accttatttc atcacattca ttaggcaaac atacgtttta taagaattta tccactttat
     2641 acacaggctg tggatatgtt ttatccctta tttttccaat cctatccgcg tagttttaca
     2701 cagtatctgt ggaaaaagaa aatcaaatat tttcatctga aaacaatgat ttttcctaaa
     2761 gtttttcata aaaagttcac agtctcaaag tagactaaag tcaacaaata agactatact
     2821 aacagtagat aaatgaatgt aattggagga ggaacacatg gatagaaaaa atgtgacacc
     2881 aaagatgaaa aaaagtaaaa ataatagcct ctggcgtaag ttaggtctgg ggcttgttgg
     2941 agggatcgtt ggaggactac tgacagctgg cattttttat gcagcgatgg gcactggcaa
     3001 caatagttct actgctacaa gcggcaatca gaattctgcc ggtgaaacag ttgtggaaaa
     3061 tgtcaaagtc aatgtagatt ctgatatcac caaagcggta gataaagttc aagacgctgt
     3121 cgtttctgtt atcaacttac aaagtcaaaa ccaatcaagc ggttttggcg gactattcgg
     3181 gcagcaagaa gaaagtagca gctcagatga tagtaatttg gaagcttata gtgaaggaag
     3241 cggcgtgatc tacaaaaaag acgggaatac tgcttatgta gtcacgaata atcacgtagt
     3301 agatggccag caaggtcttg aagtcttgat gaaagacggt acgaaggtaa aagctgaatt
     3361 agttggaaca gatgcttatt cagatttagc ggtgttgaag atcaattcag acaaagtcga
     3421 aacagttgcc tcttttggtg actcaagtgc tctgaaggtt ggtgagcctg cgattgcgat
     3481 tggttcccca ctaggatctg aatatgcaaa ttctgtcact tcaggaatca tttcttcctt
     3541 gaaccgacaa gtgacaagta cgaatgaatc gaacgaaaca gtcaatatca atgcgatcca
     3601 aacagatgca gcaatcaacc ctggaaattc tggtggtcct ttagttaata tcgaaggaca
     3661 agttatcggt atcaactcaa gtaagatcgc aagtacttct gaatctactt ctaatgtaag
     3721 cgtagaagga atgggatttg ctattccaag taacgatgta gtgaatatca tcaatcaact
     3781 ggaaaaagac ggaaaagtga cacgtccagc tctaggtatc acaatggtcg acttatctgc
     3841 cgtttctact cagcaacaag aacaaatctt gaagattcct tcatctgtaa ctaacggtgt
     3901 catcgtgaca aatgtccaaa cagctacacc ggctgaaaaa gctggattga aacaatacga
     3961 cgtcattact aagatcgatg acaaagaagt ttcttctggt gtagaacttc aatctgcttt
     4021 gtataagaaa aaagtgggag attctgtcaa aatcacttac tatgaaggct cgaaagaaaa
     4081 aacaactact gttgaattga cgatcgatca atcggcatta aaacaaaatc aaaatagtga
     4141 aaattaataa aaaagaggtt gtgacaaaga cttgtcacaa cctctttatt ttggaaatgc
     4201 ttctccgact acggcatcct caggcaatac taagattcct ttttttgctg gagcatttgg
     4261 caaatgcaat tcacgagcgg aacagatcat tccatagcta tctacccctc gcaaagttcc
     4321 tggccagatc atcattccat ctggcatcat agctcctggt tttgcaacga caactttttg
     4381 tccagcttcg atattgggtg caccgcaaac gatttgcaat actgtaccat tgtctacttc
     4441 tgtctcagta atcgataaat ggtcagaatc aggatgtggg acgcatgttt ttacgtaccc
     4501 gacaacgatc ttcggttcat gatcagcttc aagctgttct tcgaatcctg cttctttgat
     4561 caatcgattc aatttcgcta attcctcatc tgttaagctt acttggccgt tcccttcgat
     4621 tgttagatga tcggagatat ggaaaaagtt ccaagcaaca acttgacctt gttcatcttt
     4681 gatccgagca acattccatt tgcgttcaac agtgttttct gttcctttat cgtcggctat
     4741 aataaccatc aaggtgtctc cgacgtgtgc tttgttataa gcaaaaatca tgttctttcc
     4801 tccattatcc tttgctgaga tttatcttaa caaaaaattg cattcactgc atgaaatttt
     4861 tttcatgctt tatttccgaa gaagcctctt ctggaacacc atttattcgt aacaagggac
     4921 tgtgacaaga cttttgtcac agtcccttta ctgttttagt taattgatta aaaaggtcgt
     4981 acaagttcca cttgcttaat tacttggctg ttcttcttcg tcagaaacga tcaagacatc
     5041 acaaggtgaa tgacgtatca cgtagctggc tacacttcct gtcataaagc gttcgactgc
     5101 atttagacct gactgcccaa ccatgattag atcgatctca tatttttttg gcaatttgac
     5161 tgccatggat tcacgaacag aaccatatgc aataattcct tctatatttg tgtaatcaac
     5221 agatttccca tattctttac attcttcgat catttccttg gctgtttcag tttgctgatc
     5281 gactagattt tgatcaatag gtgcatagcc cataaagtta taaaattgct gatcgattac
     5341 gtttgctaca tatactttcc cattattacg gcgagccact tctaccgctt ttttgaaagc
     5401 agcaagtgct tgttcactac cgtcgatacc tactaaaata ttcttatatt cttgattcat
     5461 cttgacccct cctaacagat gtcagccttt tgttacactt tctaggaacg attcgatttc
     5521 ctgttttgtt ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac
     5581 gacaaaactt gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg
     5641 gtctacttca ataaattgcc aatcagaaaa gtcattttca atttcaggca tttgcggttt
     5701 gataaaacgg cagtctccgc accagccggc agtaaagaaa aatacactct ttccagtttc
     5761 tacgtagcct gctaattctt ctaaactatt tgggataatc attctttgtc ctctactttc
     5821 tgtttatgta tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc
     5881 tctttttata aaaaaaatct ggcaaaaagc aatcttttgc cagattttat agtttcacta
     5941 tcgcattatt ttccgtatac gatcgtattt actgttgatt tatccaaacc tttcagtgtt
     6001 tgaatcaaca tttctcgtgc agcttcaaaa tcgcgaatac tgaacatcgt ttgatgcgta
     6061 tggatataac gtcccactac gccaatcact gtacttggga ttccttcgtt ttgtgtatga
     6121 gcagcacctg catctgtacc gccttttgaa acaaagtatt gataagggat attgtttgtt
     6181 tcagcgatat ccagcaagta ctcgcgcaaa cgaggaagca tgatcaaacc tggatcttgg
     6241 atacgcatca atgtaccttc tcctaaatgt ccgaatgtgc catttttcgt cactgtatca
     6301 tctgctgcag agcagtctac tgcaaagaac agatctggct tgaatttatt gacagaagct
     6361 ttagaaccac gcaagcctac ttcttcctgg acattcgcgc ctgcaatcaa tgtatgacct
     6421 aatgtttcat tttgcaacgc ttctaaagct tctaatacca ttgtacagcc atagcggttg
     6481 tcccatgatt tactgataat attctttccg ttggctgttt tgatcgtttc agcgtaagga
     6541 acgattgtgt ctcctggaag gacaccgtaa ctcattgcct cttctcttga ttcaaaccca
     6601 gcatcgaaca aaatatcggt tacttctact gatttttgtc cgcttgttcc tcgcaataaa
     6661 tgtgggggaa cggaagaaga aatacatgga taattcttac cagtagaggt tttaagtgta
     6721 aaacgctgag cagaaacaac gtaaggattc cagccgccta gaggtacgac tttaaaaagc
     6781 ccgttttctt ggatttgtgt cagcatgaat ccgacttcat ccatatgtgc tgcgaccatt
     6841 acacggggtg catccgcttc ttcatgatgg cgcagaccaa aaatgccgcc caaaccatct
     6901 aactgtaact catctaccag cggttccata tgactttcca tataagcgcg aacatcttgt
     6961 tcaaaaccgc ttgttccttg taattctgtt aactctttga ttcgttgaaa tgttttttct
     7021 tccatatgct attcgtctaa atttcagact aaactcctct cagacttata ttgatctgcg
     7081 gataaaatat gattttttct gcagaccgat cgtatttatt atagcttatt ttttctggta
     7141 gcggttattt atttcaaaga aaatatgaga gtgtggtaaa ataagcacaa ggagtaacaa
     7201 gtgagaagaa ggaggtatgt ccacatgtat gaagaaagcg aactaagcta ttttaaaggc
     7261 gggttagcag ttggaatcgg tctaggatta ttgagcgggg tagcttcaac gatttggtat
     7321 catcgcaaaa aagtattgtc agcagaccaa gtacttgaaa atatcaagtc atcttttcta
     7381 aaagaaggtg caatcgaagg ttcttggatc tcttttgaaa aggaacctat taggaaattc
     7441 gccgttcggt cgagtgctta tcggggaggt ctttctcgaa tagaagatgg cgaaactgtt
     7501 tattatgaat ttttagcaga tgctcataca ggaaccgtat tagaaatttc aagaaaaaaa
     7561 ggtgcaacag attaatacgt caaaaaagtg agagaacgaa tacgcaccca ctattcaaaa
     7621 gagaggtatc atgtccttct tttgtagtgg gtggtttttt agatagagct ttccaaacat
     7681 atattccagt attttctttt taaataaatg atatttagcg ggaaaaacta gcatttcctt
     7741 tgattatgag gtatttttaa tttagctttt gttttctata gttaaggaga gtgaaaaatg
     7801 gaacaacaag aaaaaattgc tgttctgatt ccttgttaca atgaggaagc aacaatttca
     7861 acagtcattg cagactttaa acgagaatta ccagaagcag atatttatgt ctatgataat
     7921 aattcgaccg atcgaacata cgaattggcc gttcaaggtg gagcaatcgt aaaaaaagaa
     7981 cctcgtcaag gaaaaggaaa tgtgatccgc caaatgttct ttgatatcga tgccgactat
     8041 tatttaatgg tagatggcga tgatacttat cctgcagaag ctgtacatgg tttgctcgag
     8101 aaattacgtt ctggtgaagc agatatggtc attggtgatc gcctatcaaa tggaacctac
     8161 tttgatgaga ataagagacc tttccacgat tttggaaata accttgttag aaatacaatt
     8221 actagaatgt ataaaacaaa aattcttgat gtcatgaccg gctatcgagg attcaatcgt
     8281 atctttgtca aaagttttcc tattatgagt tctggttttc aaatcgaaac ggaattgacg
     8341 atccatgctt tagataaaaa attcaagttc gttgaaatcc caatcgatta tcgtgatcgt
     8401 cctgaaggca gcgaatcaaa attgaataca ttctctgacg gattcaaagt gatcatgatg
     8461 attattaaaa tgtttaaaga ttataagcct ttgcttttct ttgggatttg gaccatcttc
     8521 ttctttttat ttggattggt cacggggatt cctgtgattc gcgaatatat gctgacaagc
     8581 tttattacac gcatcccttc tgcgatttta agtactggat tgatgacttt agcacttctt
     8641 tcattagtaa cagggttgat tttggataca gtcgtcacga acgctaagaa agaatatgaa
     8701 ttgaatctat atcatgttta tcatgaatac cacagtagaa agtaaaaaaa ggatcaagac
     8761 tgcaaaaata atttgcaatt cttgatcctc tttttaatta attatttcgc caaacgatag
     8821 atggcatctg catagattgc tgttgctctg aacaagtcat caagactcat aaattcgttt
     8881 gcttgatgca ttgtatcagt atatcctggg aacatcgctc cataagcaac gccacgtttc
     8941 aacaaacgtc cgtaagttcc gccaccgatg atttgttcgt agcctttttc tccagtgtgg
     9001 tcttcataga cttgcaataa tgtagccacc aatggatcat ccattggtac gtaatgtggt
     9061 tccattacgc gagcacctcg agtaactgtt gcgccttctt gccccactgt ttgttcgatg
     9121 tcactttgca atccgtctac tgttacacct tttggaaaac ggaaattcat attgatgaag
     9181 ttcgcttcct ctgtaccatt ttcttcaaaa gcaaatagtc cagcattcat tgtcaatttg
     9241 cccatttttt catcttcata agcgactcct agtttttctc cataaaaatc ttcatggaca
     9301 taagcagctg ctacatggat aaattgttta gcagatccta ggaattcata gttatctaag
     9361 aacaatgcca agaaagaacc tgcattgata ccagattgtg ggcttgcacc atgtgcgcct
     9421 ttaccaacta attcgatttt tacatacgta ttgtccgctt caatcgttcc gcttactgga
     9481 tttgcttcga tgaattggta aaatgcttct tccatcgcga tagctgcatc tgcacttggt
     9541 acttgaagtg ctgcagttgc tgttcctggc accatatttt cgcgaagtcc ggaaacaaaa
     9601 gatttcaaga cataatctcc tgcattgtcg cctttgaagc gtaaagctaa agaaacattt
     9661 cctttttcac cattgatgat tgggaattca gcatctggtg agaaaccaaa atctggttct
     9721 tcttcatgtt cgaaataata agccatatcg ccccagccgc tttcttcatc gctgccgact
     9781 acgaagcgaa ctttttttga tagtttcaag tcaagttctt taatgatctt catcgcatag
     9841 taagctgcca tacttggacc tttgtcatcg cttgatccgc gtgcaaagat ctttccatcc
     9901 ttgattactg gttcataagg atctgtgtcc cagccatctc ctgcaggaac gacatccatg
     9961 tgtccaaata tccctagcgt ttcgtctcct tcgccaagat caatatgccc tgcatagtta
    10021 tcgacatttt ttactttaaa tccatcacgt tcgccataag ctaacatatg tttcaatgcg
    10081 tcgcgaggtc caggtccaaa tggtgcatct ggtgttactt ttgagtcatc gcgctcgctg
    10141 ttgacacgta aaagattttt caagtcttca agcaagtctt ctttacgtgc ttctacttct
    10201 ttttgccaat caattgtcat aaagttaatc ctccacttat ttaagtcttc gtgttcatga
    10261 taccacaaat tacagaaaaa aatagttcaa aaaatctgac aattcctttt cttatctgtt
    10321 tttctctaaa aaaaatatac gctataatga gtaaaaggag gtttttagat gctgataaac
    10381 gaacaactag tgaaaagagt catcacacct cgtccaaaaa tttcacataa aggaacattt
    10441 ggacgtatcg ttttgattgg cggcaatgaa caatatggcg gagcaatcat tatgagtgct
    10501 ttagctgccg tacacagcgg agcgggtctc acgactgtag taactgatga aaaaaatcgg
    10561 agtgctcttc acagtcatct gccagaagca atgattgtag tttggacgga tactgaccag
    10621 tttctttccg taataaagca agcagatgta ctcttgattg gtcccggatt gggtacttct
    10681 tctaagagtt tggaacgttt caaacaagta ctagaaaacc aatcaaaaga acaatggctg
    10741 gtgattgatg gctctgcttt aagtttactt gctgctaatc atcttttgct accctatccc
    10801 gaacaaactg tacttacacc tcatcaaatg gagtggcaac ggttgagttc tctaccgata
    10861 caagaacaac gggataaaga aaacaaaaag aagcaacagg aaatcggtgc tgtgctcgtt
    10921 ttgaaaagtc atgagacaaa aatttatggt caccagcact tctacaccaa tcctttaggt
    10981 tctgctgcta tggcaacggg cggcatgggt gacactttag ctgggatgat cacaggattt
    11041 ttagcccagt ttcctcaaaa tgaccaaacg attgcagcag ctatttatct tcatagtttc
    11101 attggagacc agctggcaaa agaacaatat attgttttgc cgacacaaat cagcgaagca
    11161 cttccgaaat ggatgaatta tttttgtaaa aaatcaatgg aaaacgaggc atgagtcatg
    11221 gagaaattta caggttatgt gcgagaacct ttttattatg aaacagatca aatggggatt
    11281 attcatcaca gcaattatat ccgctggttt gaagaggctc gggtagcctt attggtacat
    11341 ctagggtttc cctataaaaa aatcgaagaa tctgggatca tcatccccgt tctggaagta
    11401 aattgcagct ataaagaaat gatccattac ggagaaaaag tgagcatcca tccaactatc
    11461 caaaagtata cggggacacg gttggatttt agctacgaaa ttgtgggaac tgaagacaaa
    11521 aaattaaaaa ctactggcag tagtaaacac tgttttttac tttctgattc gcaacgatta
    11581 gtgaagctga gcaaagtcaa tccggagtta gatcagttat tccaagcgta cgcttctttg
    11641 gatgatgtaa attcttaata aagaccgtct aagcattttt cttgtattgt gtcgagtagt
    11701 tcgtataatg aaaagaaaaa agcaggtgat ccccatgtat gtcgttaaag taatgcacgg
    11761 ttacatcgat aaaactggct gtcgcactcg ggaaaaaaac ttagacaatc tgctcatttt
    11821 caaagacaaa aaagaatccg aagcttttgc taaaagaatc ggcggtcgtg tcaaaccgat
    11881 ccaagaggta cgaccagatt gattcaatat ttttcttttc gaggctctaa taggcatgcc
    11941 tatcagggct ttttttgcag actgtgtttt tatgcccaca cgatttgtaa gcgatttatg
    12001 ttataataga ttatatctgc atagaaagga aggttaaatg gttatcaata atttgcaaca
    12061 gcaagcttat gaatctatac gtaaacaaat catttattcc gagttagcac ctggaagtaa
    12121 aatctccgat aaagtgttag aagaacaact agctatcagg cgtacaccga ttcgagaagc
    12181 actgatccaa ctgcgcaatc aagagcttgt aaaaaccatt ccacagtccg gcacttatgt
    12241 ttcaaaaatc gatatccgat ctgcttcatt agcccgttat acgcgggaaa agttggaaaa
    12301 accgatcctg caagaatgca gtgcgaaaat gaccaagcaa gatcaagcta aactagaaaa
    12361 aatactagaa caaaccgatc aggcagtaat cgctcaaaat aaaaaagctt tttttgattt
    12421 ggatactgat tttcaccgaa cttgttatga aattgctgga aaacgagaaa tttgggattg
    12481 gctagaaagt tatagtacgc acttgaaccg tttcagatgg ttacgtttga cgatttcaga
    12541 attagactgg ggtcgagtat tagatgaaca tcagacaatg cttcaatcaa tgattgatca
    12601 taactttgat gaagtcggat ttctttgcac gatgcattta catatgatta tcgaggagca
    12661 agaatacgtg attcataatt accccgatta ttttgaagat attcaggaca gacccatcaa
    12721 ataagaggtt ttggaatcag cgttttgacc tgagtattag agaaagaaac ggcaaaatag
    12781 ttcctcatat tttgccgttt tttttgagct aataccgagg gtcagctgat tgcacaccgt
    12841 ttattgagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa
    12901 tagcttctta tgtttttaac ttggctggac ttattaccgc agatgcaagc ttttgaacac
    12961 cgttttccaa gaggtcgtga gcctgccgtt catctccgag tcacaaggaa caatttcaaa
    13021 aaacagcttc tcatgtttgt tgaatatttt gacttgtgac cgaggagttg acaggagaac
    13081 accgtttatt cggaaaaaaa ggactgtgac acgacttttg tcacagtctc tttatttgta
    13141 atctaaatca tactccgtac atcttttttc tatttttttt atttcttcta agatcccttg
    13201 gctacgaatt tcattatatg gccaaagtaa gactgcctct aatacataag gcgaaaaatc
    13261 gctttgattc atctcttttg aaaaactcat cgctgctatc tcgctcaatg cacgaatgtt
    13321 tgattgatgt gtataaaaag gcaaccgcca cagcacaatt ttttcgcttt gcgtataaat
    13381 tcccttaaca gaagcttcga tgtggtgaaa tagttcatgt ccaataatca aagcttgcgc
    13441 tgtttcttta gagatcgaag ctagtcctga acattttaat ttttctaacg gctccgtcat
    13501 caatcgaatc ccttcatctt ctgcataagt tgcaaaagtg attcgttcgt ttatttgctc
    13561 tgattcacag gaaacgattg gaacagccat ttgttcacaa atttctccta catcggcaaa
    13621 agggtacttt tttttgatgc tacatgccgt ttcgatacca caatcgattg attgttgtat
    13681 aagaattttc tgcttggaca aaggaatctt tttccaaaat aaatctcgcc gaaactggta
    13741 gaatccccaa atttcttcgg ggatctgttg taattcgttt agtaactctt ctaacttatg
    13801 tttgtttgat atttccggca acgacgatca cttcttcctc cggttccata gtagatactt
    13861 ccagatccat caaaagctgt agttgttcca gatccgtcgc tgtaaaatca gagactcttg
    13921 ctcctaagca gagataaaat tctggtcggg caatcaattc agtttgataa actgccatat
    13981 cttcccaaag ttgttcgaca gctgtcatat aattggctgc agttgttttc atacatgaag
    14041 catgtccacg ttggaccttg ataaatcctt tttgatcgat gatccgaaga cttccagcat
    14101 tttttgaacg attctgttcg ccgcatacat aaaagaaagg tgtattttcc aataagcaaa
    14161 cttcattttt atttaatcgc atatcctctg cagcaagctc tagtgcttct tctgttgtga
    14221 tctccttggt tagatcggtt gctttcactt ccgtagaacc agtagcgata gccgttactt
    14281 ttgaagtctg tggatcgatt tctacgtgga cttcgatcga ttctggtgta gcgcctgatt
    14341 caatcgcttt attcatcgct tcatttttca acgatcgtat atcttcttta cttggcgaag
    14401 ggatgatacg ttctacaaca tctcggacca ttgcaagtgc tacaccaata gaagaaatca
    14461 cttcggcgtt ttcaggaatc gaatatttca cacccatttt attactaaag taagtaatca
    14521 atgaggcagc gccgccccct acacctacaa gagaaatctg atctttctcc aagtgatact
    14581 tgtctgccaa ttctaaaatc accggttcaa tcttagcata ggatttttcc atgatctgtt
    14641 ctgcgatgtc ttcaaccgtc gtatgacaat aatcagctaa tgcttggatg gcttttcgtg
    14701 cagatgggac atttccataa gaaaaatgtt cttcttgcac taatccaagg acattggctg
    14761 cacatgtatt tgtaatagtc acttcttctc cgtcttccat cactaccttg acgtaatcag
    14821 cagggtctcc ttctttagga gagaaaaatt caaccttagg acctttgatt ttttctgtct
    14881 ccgtaaatac tgcataatct agaccggcaa tatgtgcaga acgtggaccg acatcgatga
    14941 ttcctgactg attagcccgt accatcgaac cgcctgccac acctagaacc cgaacatcta
    15001 atgaagagat atatgttggg tggccaccta caattgaata atcgattgct ggacgtccat
    15061 ttttaatgac cccgatattg gttgttgttc cgcctacttc aaaataaaca ccattagacg
    15121 ctctcaagta catcaaagag cccatgactg atgctgctgg accggaaagc atggttaaaa
    15181 caggtcgttt tttcatttca ttgatttcca ttactccgcc gtctccgcgc ataatcatca
    15241 aagaaacatt gacccctgct tctcgtacgg aatcttctgt agaggttgcc gtatctagca
    15301 tttttggtaa aatagatgca ttgattgctg ctgtccgagt tcttcttgtc aatccatata
    15361 attttgtgat gtcagaagcc atcgttgtag ggatcgattt taccgatgct gcttcataaa
    15421 caactcgttc tggttcccca ttatctactc caaatgccat agaagaaacc aagacctccg
    15481 ctctttctct ttctaaagaa gaaatcgttt ctgaaacacg gtctacattt aagtgtttga
    15541 caggtaagaa tgcattgaca atctctattt ttttcttatt ccctaaatcg atatcgttca
    15601 atctagtttg tcgttttgct aaaaaacctt ctaatccacc ttttgccatc ccgattacgc
    15661 cgacttttgc tacgtctcct tcaatcagtg cattagtcgc ttgtgtggta gaatgtgcaa
    15721 caaacacaac atcttctgga ctgatattat tttccagcaa acaattttga aaagactgaa
    15781 cgacacctgc ggctactcca cggacatcat cgtgggttgt tttcactgaa gattttccga
    15841 tgatttcgtg cgtcgcatta tcgattgcga ctgctttcgt atgtgtaccg ccaacatcaa
    15901 ttcccattcg cacaaaacgt ttcattttct tttcctccat tcatctaatc gtttttatcg
    15961 acgttttgtt tactaggcgt tcccgtacaa taagaacgtg atcaggcata agatcgctcc
    16021 gatgatccaa ccagttggga tggatttctt catatattct cgagaagtta cttttgtata
    16081 acctaatccc caagcaaccc aagattgtgt aatatctaaa tgctgaggag caatcgttgt
    16141 gattgcaaat aacgggaaca aaaaggctgg cgacatcgtt ggattaacag ctaatacgac
    16201 agctaaaata gctgagcctg aaccgactag attcattgga ccgcggaaaa atcctagtgg
    16261 tgtcaatata gcaaatacga ttgccaagac taatggtgat tgcggtatcc aatctccaaa
    16321 aatagctgaa aaataaggag aagcatacgc agctgaatta ttgaacatcg ctaaggtcat
    16381 caagaaacca accattggcg caacatctac tgcgccatct gtaaataatt tcgcaaactg
    16441 acgacagatt tctttataac ctccgcgcaa ttttccagta aacagcaatg cccagatacc
    16501 tgctagaata aaacctaaga tgattggaag atctaaaaga acgaccccta aaactggtaa
    16561 taatacggct aaccatgaga tcattggtgc gtcgccacct tcagctggta cgtttgctgc
    16621 cattgcatag cgtttctttt tgttcattga tatattagcg accgttagta ccacaactaa
    16681 gcttacaatc atcccaatca taccaatttg gaaatagtca ttataagtat agctttcagc
    16741 cgctggattg aaaccagcat aaatcgtttg atattgtttg aaattgacga tattagcaaa
    16801 gattccagcc ataatcgagc ccataaatga gaacagtgca accggtactg aaatacctaa
    16861 tgacatcaag attggcaaca caataactgc aatagaaatc actggtccga ttcctgtcat
    16921 cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc ttggcttgtc
    16981 ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat ctactagaac
    17041 acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt actcagctgg
    17101 ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca tcgccgggtt
    17161 tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca tgatggcaaa
    17221 gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga atgagataac
    17281 taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt gtttttctga
    17341 taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg ctttcattaa
    17401 agggtgacca taaatgttca gttttcaatt aaaaaacctt aattatgcta ttttagactt
    17461 atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga ggaaagaaca
    17521 taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc caaagcttct
    17581 tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa gtcagctaac
    17641 attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt tgagtagctc
    17701 aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg gaagtcgcgt
    17761 ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg ttcttttgct
    17821 gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag cactctttta
    17881 cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc ctcctaataa
    17941 aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta gttgttggcg
    18001 aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac ggcgttgttt
    18061 ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca atccgccttt
    18121 accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg gcatgactat
    18181 atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc agttagtcat
    18241 tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg tccgtccatt
    18301 ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg attcaagact
    18361 ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga agctttcact
    18421 ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa gtcattcaag
    18481 tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt tttacgcgct
    18541 tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat tcgcgcaact
    18601 ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc tgcaatattc
    18661 aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat caatcgtaac
    18721 tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat tcacctccaa
    18781 atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg agttctctta
    18841 cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc cagtgacatt
    18901 atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca tagtatagca
    18961 aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct gaaaatgatt
    19021 agattagcag actttctagc agtgattccc gcttctttgc tatactaaag aaaaacgagg
    19081 tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct tacaaaggga
    19141 gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg gggtgaccaa
    19201 agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa acttacgatg
    19261 caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa atgtgatttt
    19321 tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca atgtaaatta
    19381 acaggtacga attttgccga aagttatctt cgagactgta ctttcactga ttgtattgct
    19441 tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca ttgtcaatta
    19501 gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga caatcagttg
    19561 accggttcga actggtttcg aacttctctg aagggattag actttacgac taatttcttc
    19621 acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca agagcaagca
    19681 cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga atccgccgcc
    19741 acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg attccccatc
    19801 cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct gtcacaatag
    19861 ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca ttcaagttgt
    19921 tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa gctaagttcg
    19981 tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct ttttcctttt
    20041 ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact acattttgtt
    20101 ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc agcagattct
    20161 tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta ttcacttcct
    20221 cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat aaactgtata
    20281 cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg tactcggtat
    20341 tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata ggactagttt
    20401 cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt aacgcttcat
    20461 caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa gtcagcatct
    20521 cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg ttttgctgta
    20581 cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata ctctatcctc
    20641 ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta ccctttattt
    20701 gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat ttttcttcga
    20761 tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag cggtctccat
    20821 cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa cgtctgttca
    20881 attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac gaacgaccac
    20941 gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact aataaatccg
    21001 gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc agttcatcat
    21061 acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg aaaagcagtg
    21121 aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct tgttttatgc
    21181 ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt tcagggtttg
    21241 cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc tgactatcaa
    21301 agtgttcagc catcatttct gttaaacttg attttccggc accaattgta cctgctaaaa
    21361 cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt aactccaacc
    21421 tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta gtcaaagaag
    21481 aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac gtattcttag
    21541 ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag acccttagtg
    21601 gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt tgcttaaaaa
    21661 tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag aatcaagcgg
    21721 aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga ttgcctttcc
    21781 tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg acgatcaact
    21841 ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa aaaatatcaa
    21901 aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc aactgaccat
    21961 tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg ccacagcagt
    22021 cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac tattagaaaa
    22081 tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg cagccgctac
    22141 tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact tttctttgaa
    22201 catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa gagaagccgt
    22261 caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa tgatccaaaa
    22321 attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac cagaaacgtt
    22381 ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca gcaatgattt
    22441 tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca aattaactgg
    22501 cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag cccaagaaat
    22561 cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat taggagtaca
    22621 tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc aaatattggg
    22681 gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta acattggatg
    22741 cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat gaattttatc
    22801 tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt cttgaccttt
    22861 ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat ttcgttccta
    22921 ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc gcttgcaata
    22981 ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga cgcgggtctg
    23041 gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc catgacgact
    23101 taagttctta cgctaagcca gtcccttccg attctttcga agacgattta gcaatggttt
    23161 tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg cgtcggacag
    23221 tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat ctatatcaat
    23281 tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg gaaagaaacg
    23341 gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac tggtctcctg
    23401 acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt ccatgctact
    23461 ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat ttatctgagg
    23521 tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca catgctggtc
    23581 caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct gcaccaggca
    23641 aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca gttatcgctg
    23701 cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga agtatccaat
    23761 ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa ttagttttag
    23821 atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact gaaaagtatg
    23881 cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt gagttagaca
    23941 gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt gcaacggtca
    24001 aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc aagattgcag
    24061 cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct gctagctgtt
    24121 atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa caagaaactc
    24181 agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt gagccattga
    24241 ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc tctacttctg
    24301 atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat cagcttttct
    24361 taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa aataatgtaa
    24421 cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta tctgcaatca
    24481 ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct gaacagtatg
    24541 aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta attgttgacc
    24601 agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc actccactgc
    24661 cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag acgaacatgt
    24721 ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg tacggatcat
    24781 ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac aggcagttgg
    24841 gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg aaaaaacaaa
    24901 aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga ttgccactgg
    24961 ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga ttatgcgaca
    25021 agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg tcgaaagagc
    25081 caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga atgcacctca
    25141 agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac ttattcaaga
    25201 aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg gcatgacaag
    25261 agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca gcggtcgaag
    25321 cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac tgagttattt
    25381 agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag cagacacttc
    25441 tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta aagccctttg
    25501 tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga tgaatcacgg
    25561 cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc tgctttatac
    25621 tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct tttccggacc
    25681 agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa gacgcacaga
    25741 aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtacaaacg
    25801 gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
    25861 ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc ctcttttttc
    25921 gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg atttcacaac
    25981 ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat aggcaagtat
    26041 gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag aagccaagaa
    26101 gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg tacgtccaac
    26161 gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta atggagcagt
    26221 ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt attgaccgta
    26281 ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag cataagcact
    26341 gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt taccgccgcc
    26401 tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat ttaaatccac
    26461 gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc gtttgatgat
    26521 ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct gttgaaccat
    26581 cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat cgatgcctgc
    26641 ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat taaatacgat
    26701 ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat agacagcacc
    26761 ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt ccatcatttt
    26821 ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct gtataggtga
    26881 tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca cctgaatgga
    26941 tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt tttgtttcat
    27001 ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca aagtagacat
    27061 aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg cttcctggtt
    27121 ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga tggatgtatt
    27181 ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt tttacttggt
    27241 cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta atatagaatt
    27301 tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct tttccgatat
    27361 agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg aatcctggtc
    27421 caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca cctagttctt
    27481 tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag atcattcgat
    27541 cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct actaaaaatt
    27601 cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt gtataacgtg
    27661 tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca gagccgcatg
    27721 ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg atcgtatgct
    27781 tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata ttggataaaa
    27841 tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc acgattgggg
    27901 tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc cccttcaatt
    27961 cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga atattcggga
    28021 acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct tctggcgctt
    28081 tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata tctactaaag
    28141 gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt ccacgatagt
    28201 tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc tgtttcgtca
    28261 ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat atatctattc
    28321 cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa cttgatccgg
    28381 cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata taataagtac
    28441 ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat tgcaagtgct
    28501 ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc gcttcatgtc
    28561 cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg gcagcgaatc
    28621 gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg atttctccga
    28681 gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt gctgcatact
    28741 gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct aaaaggttcc
    28801 cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg tccagactca
    28861 caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca gaaatgatac
    28921 ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt ttcagcgctg
    28981 ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac agattgcaca
    29041 tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca gctaagatag
    29101 ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt tctggttggt
    29161 ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact gtaaaagtca
    29221 actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa gcttcttctt
    29281 tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat aaaatatcaa
    29341 tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa cttggaaatc
    29401 tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat tatttttgaa
    29461 ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt tcttttgtct
    29521 catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt tgttcgtttt
    29581 tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg tcaaatgcga
    29641 tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg gtgatcgtat
    29701 attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc tcttcactga
    29761 attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg attccgattt
    29821 ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac aagatctcat
    29881 tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc tgttctaaaa
    29941 acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg tagcgaataa
    30001 gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg atcatcaaca
    30061 aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt gcgatcgtct
    30121 gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct ttgatcaata
    30181 cagttccata aggcttttta ttttccatat aatattctcc tatttcttta taagaaaaat
    30241 tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc atttctcttt
    30301 acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga tataaagcaa
    30361 tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct cgcaaagcat
    30421 cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg gtttctggga
    30481 ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt acatcttcta
    30541 aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg tacttttctc
    30601 cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac gctacgccat
    30661 gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt cctaaaccga
    30721 catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct cgcccatttt
    30781 gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct tttaaatgca
    30841 gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca tgcgtcatag
    30901 catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata tcgctgtcga
    30961 tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg tgatttactt
    31021 catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc gttggtatag
    31081 ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta atgacgtctg
    31141 aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt ggcgaacctc
    31201 caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact ccatctttta
    31261 cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg attgctgctt
    31321 tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct ttgtcagtaa
    31381 tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac tcaatcgctc
    31441 cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc tattcctcct
    31501 aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt tgcccagttg
    31561 cttgttgcta cagttcaaag actggcatca attcccaact gactggagag gcatctggat
    31621 ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat gtatttgcca
    31681 ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa cttgtactcg
    31741 gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta cgcatttctg
    31801 gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa actttcatgc
    31861 aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc caaataacct
    31921 gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc cgtgattgtt
    31981 tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt ttctgctgtc
    32041 tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc atgttgtggc
    32101 cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat ctcatttgtt
    32161 cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa gcattccgta
    32221 cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt aaaactcatc
    32281 gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc tacttctaaa
    32341 cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca ttcgttttcc
    32401 agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat tccaagattc
    32461 tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac aataaaatag
    32521 catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac tttttcagga
    32581 tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc gccatttctt
    32641 tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt cacataaggc
    32701 gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta attccaacac
    32761 gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa cttgattttt
    32821 gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt ccgtataagc
    32881 cattcttgaa gtaaagtcaa aagccagctc catttcttcc aatcgctcaa ctggttccag
    32941 catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag cagcgatacg
    33001 gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca attcgttacg
    33061 gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat ggtctgaatc
    33121 ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag ctaatgaaga
    33181 taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac aaacaagttt
    33241 gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat aagcttcagc
    33301 gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat ctaactcttc
    33361 tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac gatcaatcgg
    33421 attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc cagtttcttt
    33481 cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt attcacgaac
    33541 ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg aaaagaaagt
    33601 cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt tttcaaaatg
    33661 tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga tagcatgaag
    33721 gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt gtctaagctc
    33781 ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac cggtcaattc
    33841 gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca tagaaatttt
    33901 gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg catatttttc
    33961 ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt gattttattc
    34021 acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc acatggaaac
    34081 gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt actgagcatt
    34141 tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac aatgattccg
    34201 gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc tacgatgtgc
    34261 agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg tgcgtaacaa
    34321 agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg gactggctgc
    34381 tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt caaaaatcga
    34441 gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag ctctgccaat
    34501 tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa ccacatcccc
    34561 atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt ataattttca
    34621 gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag cgaccatgtc
    34681 ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac agctgacgcc
    34741 gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt tgaaaattgt
    34801 tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa taattccttc
    34861 gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc attcagcagc
    34921 tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac ataccctaaa
    34981 taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc ttcagcaaac
    35041 aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata caacttatct
    35101 agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc ccgataagca
    35161 atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac tgcccatgta
    35221 tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa tccaatcaaa
    35281 atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt ccgaaagccg
    35341 tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct cgtacttttc
    35401 attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt catttctcca
    35461 cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat ttattgacaa
    35521 tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata taattgcatt
    35581 atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc actcatggtc
    35641 ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt tattttttta
    35701 tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac gagcacattt
    35761 accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat ataatgataa
    35821 tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat atcagcgtta
    35881 tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg gtcagaacgt
    35941 ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt caacaggaga
    36001 tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc aacaaggcac
    36061 gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga atgaactgat
    36121 tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg tttacaaact
    36181 gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat tagaacaaca
    36241 attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg gttctccacc
    36301 aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc ggtatacaga
    36361 atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt actttagccg
    36421 cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca gctgattcat
    36481 ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa taaaataacc
    36541 gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta gtgatcattc
    36601 tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat cacgtttcaa
    36661 aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat gcggataaga
    36721 agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca acaagtgctt
    36781 ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag tgtacttttt
    36841 aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt cctctaaaga
    36901 aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac gctcattagg
    36961 aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat tgagaaaaat
    37021 caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt ttttatcagg
    37081 aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt gcaatacacg
    37141 actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg gcgtggaacg
    37201 actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat ctgaagattg
    37261 gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg ctttgatcgt
    37321 aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat ggatctcata
    37381 gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga catgatagat
    37441 ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct tttggagcat
    37501 gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt ttttgtaatc
    37561 aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc ttctagcccc
    37621 gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc aagaagactt
    37681 cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag gtacggacga
    37741 ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc caaatttacc
    37801 ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca tatcttttca
    37861 ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc actacttgtg
    37921 atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc ttggatcagc
    37981 tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat ccccaccatc
    38041 atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc ttcagcaaat
    38101 gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca tccagtttcc
    38161 gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc gaactttgtc
    38221 agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca acttaaaatt
    38281 cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat cccagaattg
    38341 atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag ctgcagtgta
    38401 tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac ttcggaaaga
    38461 tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca tattgggtag
    38521 ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga taaccccata
    38581 aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac ttgcaccgac
    38641 atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca tgattgaaaa
    38701 caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa atattggtgt
    38761 aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata acacaagatt
    38821 gactaataac agaataataa atttattatc cgttacgccc ataatcaacg aactaattgc
    38881 ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag ccaataagaa
    38941 catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat cttttagttt
    39001 taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta tcgctgaagc
    39061 ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa tcaatagaag
    39121 gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt tttcgacaac
    39181 agggtattta ttctttttag caacgatgaa tgctactagc atgattccta gcgcccacaa
    39241 agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac cagcaacaag
    39301 tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag ctgacgcgat
    39361 attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag ggatcaacac
    39421 tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa ataaagcatt
    39481 ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca acatcgcaaa
    39541 atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga gaacgaaaaa
    39601 cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag ctgaactaaa
    39661 agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga atgcaatact
    39721 tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata acactaatcc
    39781 ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg ctggcttgtt
    39841 gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc ccgatacagg
    39901 tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct gccccattgt
    39961 caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata ccagaaataa
    40021 actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt cacttaaaac
    40081 cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca tggcaaacca
    40141 gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta aaaccgttct
    40201 ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag ctcccaggat
    40261 ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc ttggatcttt
    40321 tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg ggcaactgcc
    40381 tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc ttgtgctaaa
    40441 gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt tgattctttc
    40501 gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat ggcatcattc
    40561 atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt agccacacca
    40621 ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat caaattggat
    40681 tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc taaaagctct
    40741 accattttga tagcggtttc gctttgcatc acacggatct tctttccctt caaatcttcc
    40801 ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc ataataggtc
    40861 aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat ttcttcatta
    40921 tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga aaaaatcgaa
    40981 taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc tactgctcct
    41041 gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg gaaatactgg
    41101 atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt cattgcatca
    41161 attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc atttacatct
    41221 gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc tagcagacct
    41281 aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt ttcctttctt
    41341 ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact gtccataagg
    41401 cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc ctgtcccgat
    41461 tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt ggatagcacg
    41521 ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt tcaaaatacc
    41581 gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat ccaacaacgt
    41641 atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg cttcaagtgt
    41701 ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt caatgatctc
    41761 tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg catagttatg
    41821 atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca tcaagtattt
    41881 gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat tcaacagttt
    41941 cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca actgattctt
    42001 attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag gcatgtcatt
    42061 catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt tcgtgtcttc
    42121 atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg gcggagcgcc
    42181 ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag gattttttgt
    42241 catctggtaa tttttataaa taccatatag accgacacct tgtggccaat tccacactgt
    42301 ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac cattatcgtc
    42361 tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta attctttagg
    42421 tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc tttcatttta
    42481 tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa caaaaactgt
    42541 ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg aaattaaaaa
    42601 aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt atcttttttc
    42661 tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca attcagagat
    42721 caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt cagccatacg
    42781 gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac tcatgttgat
    42841 gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta attcctctgt
    42901 tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat ctgcacggaa
    42961 atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat agcggataat
    43021 atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc cgccgatttt
    43081 atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac cagccattgt
    43141 agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg atttttctaa
    43201 atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat ctgaagccat
    43261 gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat ctgctgtgac
    43321 agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg gcgtcaatgc
    43381 ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg caaatggcgt
    43441 tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt tttctggttt
    43501 gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac caaagaaaga
    43561 tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg cagaaccgcc
    43621 tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc ctgcgtatac
    43681 ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca catcattttt
    43741 acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca taccagggtc
    43801 acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata gagagttctt
    43861 ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc gtttcgctac
    43921 agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt tcttaccata
    43981 agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac cgatgccgcc
    44041 ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac cgaatttgac
    44101 atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc caagattcaa
    44161 cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca tggcaagaac
    44221 tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca attcagcaat
    44281 cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt ccatcgcaga
    44341 atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg catcttctaa
    44401 tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt ctgcttttac
    44461 tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa tcattccatt
    44521 atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg ctgttttttc
    44581 aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg tagagtaagc
    44641 agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg gatggttcat
    44701 tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat tttcaggagc
    44761 tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag atttttgggc
    44821 acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca ttgatttgaa
    44881 aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac cgactggttc
    44941 agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg ttttatcatg
    45001 tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt tatcttcgta
    45061 aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt gatccaaagc
    45121 tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc tttccatttc
    45181 tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta cgtcaattgt
    45241 cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg gtcatggtta
    45301 tttgttaaat aattcacatg tatatcatac aatatttatt tttctat
//
LOCUS       DAHHPA010000010        43453 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.010, whole genome shotgun sequence.
ACCESSION   DAHHPA010000010 DAHHPA010000000
VERSION     DAHHPA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.010"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            401..1424
                     /locus_tag="NBH55_000531"
                     /pseudo
     CDS             401..1424
                     /locus_tag="NBH55_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1767..2023
                     /locus_tag="NBH55_000532"
                     /pseudo
     CDS             1767..2023
                     /locus_tag="NBH55_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2410..2637
                     /locus_tag="NBH55_000533"
     CDS             2410..2637
                     /locus_tag="NBH55_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCD1508546.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2653..3819
                     /locus_tag="NBH55_000534"
     CDS             2653..3819
                     /locus_tag="NBH55_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCD1508547.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            4054..5097
                     /gene="hrcA"
                     /locus_tag="NBH55_000535"
     CDS             4054..5097
                     /gene="hrcA"
                     /locus_tag="NBH55_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCD1508548.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            5191..5754
                     /gene="grpE"
                     /locus_tag="NBH55_000536"
     CDS             5191..5754
                     /gene="grpE"
                     /locus_tag="NBH55_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCD1508549.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5805..7634
                     /gene="dnaK"
                     /locus_tag="NBH55_000537"
     CDS             5805..7634
                     /gene="dnaK"
                     /locus_tag="NBH55_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCD1508550.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7785..8951
                     /gene="dnaJ"
                     /locus_tag="NBH55_000538"
     CDS             7785..8951
                     /gene="dnaJ"
                     /locus_tag="NBH55_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCD1508551.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(9319..10056)
                     /locus_tag="NBH55_000539"
     CDS             complement(9319..10056)
                     /locus_tag="NBH55_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD1508552.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(10058..11560)
                     /locus_tag="NBH55_000540"
     CDS             complement(10058..11560)
                     /locus_tag="NBH55_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCD1508553.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11819..13813
                     /gene="uvrB"
                     /locus_tag="NBH55_000541"
     CDS             11819..13813
                     /gene="uvrB"
                     /locus_tag="NBH55_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCD1508554.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13823..16639
                     /gene="uvrA"
                     /locus_tag="NBH55_000542"
     CDS             13823..16639
                     /gene="uvrA"
                     /locus_tag="NBH55_000542"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCD1508555.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16902..18167
                     /locus_tag="NBH55_000543"
     CDS             16902..18167
                     /locus_tag="NBH55_000543"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCD1508556.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            18171..18902
                     /locus_tag="NBH55_000544"
     CDS             18171..18902
                     /locus_tag="NBH55_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCD1508557.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            19037..19921
                     /gene="rapZ"
                     /locus_tag="NBH55_000545"
     CDS             19037..19921
                     /gene="rapZ"
                     /locus_tag="NBH55_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCD1508558.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19918..20916
                     /locus_tag="NBH55_000546"
     CDS             19918..20916
                     /locus_tag="NBH55_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCD1508559.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20950..21885
                     /gene="whiA"
                     /locus_tag="NBH55_000547"
     CDS             20950..21885
                     /gene="whiA"
                     /locus_tag="NBH55_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCD1508560.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            22453..23109
                     /locus_tag="NBH55_000548"
     CDS             22453..23109
                     /locus_tag="NBH55_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508561.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            23288..23853
                     /locus_tag="NBH55_000549"
                     /pseudo
     CDS             23288..23853
                     /locus_tag="NBH55_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24552..25073)
                     /locus_tag="NBH55_000550"
     CDS             complement(24552..25073)
                     /locus_tag="NBH55_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCD1508562.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            25271..28951
                     /gene="nifJ"
                     /locus_tag="NBH55_000551"
     CDS             25271..28951
                     /gene="nifJ"
                     /locus_tag="NBH55_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCD1508563.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            29140..30027
                     /locus_tag="NBH55_000552"
     CDS             29140..30027
                     /locus_tag="NBH55_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCD1508564.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            30024..31193
                     /locus_tag="NBH55_000553"
     CDS             30024..31193
                     /locus_tag="NBH55_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508565.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            31217..32572
                     /locus_tag="NBH55_000554"
     CDS             31217..32572
                     /locus_tag="NBH55_000554"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCD1508566.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(32644..34449)
                     /gene="glmS"
                     /locus_tag="NBH55_000555"
     CDS             complement(32644..34449)
                     /gene="glmS"
                     /locus_tag="NBH55_000555"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCD1508567.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            34859..35149
                     /locus_tag="NBH55_000556"
     CDS             34859..35149
                     /locus_tag="NBH55_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508568.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            35162..36187
                     /gene="sppA"
                     /locus_tag="NBH55_000557"
     CDS             35162..36187
                     /gene="sppA"
                     /locus_tag="NBH55_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCD1508569.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            36190..36849
                     /locus_tag="NBH55_000558"
     CDS             36190..36849
                     /locus_tag="NBH55_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCD1508570.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            37006..37938
                     /locus_tag="NBH55_000559"
     CDS             37006..37938
                     /locus_tag="NBH55_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508571.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            38092..39381
                     /locus_tag="NBH55_000560"
     CDS             38092..39381
                     /locus_tag="NBH55_000560"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCD1508572.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            39628..40878
                     /gene="clpX"
                     /locus_tag="NBH55_000561"
     CDS             39628..40878
                     /gene="clpX"
                     /locus_tag="NBH55_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCD1508573.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            40961..41548
                     /locus_tag="NBH55_000562"
     CDS             40961..41548
                     /locus_tag="NBH55_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCD1508574.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(41622..41777)
                     /locus_tag="NBH55_000563"
     CDS             complement(41622..41777)
                     /locus_tag="NBH55_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508575.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            41913..43241
                     /locus_tag="NBH55_000564"
     CDS             41913..43241
                     /locus_tag="NBH55_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508576.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            43252..>43453
                     /locus_tag="NBH55_000565"
     CDS             43252..>43453
                     /locus_tag="NBH55_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508577.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENK"
BASE COUNT        14405 a         7404 c         9358 g        12286 t
ORIGIN      
        1 aaaacacagc taaaaattag ttgtgttttt tattaggttt acttttctgg tataaaagga
       61 actagctact ttttcaaaat ttctaaatca ttcaatatgt agcgcttact aaattattcg
      121 ccataaaatt ttataattgg atcactcatt gttttaccta agtcatattt aagtaataac
      181 cggtgttttt acataagaag acttcgggta ttttttagtt tgattagctc tgatatacct
      241 ataaagaata tgctaaggta agaatagtaa aaattcacat agatgaggta taataatgat
      301 atttttatat attacagata acttgacaca aaatgagttt ttttcgattt ttcaagttat
      361 ctaatgcaat tatattgata tcattaggag ctgcattgtt atgaatccaa cattgattac
      421 taagaaagaa ttactaaaaa aattagatat ttctactggt gtattagcta atttaattag
      481 aaatggcatg ccaaaagagg gagaaatgtt taatcttgat aagattatta catggcgaga
      541 aaattggtct aaaaacatat taggtgagtt agaagttggt agagtatata ctaacaaaga
      601 aatttcagag aagtttaaat gttccaaaca gggtggaatg agaagatctc atcaaactaa
      661 tactcttgta ctgtttagtg atcaaacagg aagtaatgtt tataaagata agtggttaaa
      721 tggtatttta caatataccg gtatggggct aaaaggggat caagtattgg ataagaatca
      781 aaataaagtt ttagccaatt caaaaagtaa ttttgtaaag attcatttat ttgaaacatt
      841 taaaccaaaa gaacacacat atctgggaga agtatattta gcaggacaaa tatatactgt
      901 taatgaaaaa gatagttcgg gcaacagcag aaaagtatat aaatttccat tagctttaat
      961 aaatcaagag caattgattg aagataaaga tatataacca ggaagaaaat caaactagac
     1021 atattcgtaa tttatctaat gctaaattag aggaagaagc aagaaaagtt agtaattata
     1081 ataaaaaaat ggctgcaaaa tatgttaacg aagaggagtt ttgcttctca aaaacaaaaa
     1141 tatataaacg taatcccttc gtatctgaat atgttaaacg acttgcaaaa ggtatttgtc
     1201 aactttgcca agaaaaaggg ccatttataa aagacggtgt accttattta cattgtcatc
     1261 atatagaata tttgtcgcaa ggtggaaaag atgtgattga aaactgtatc gcgttatgtc
     1321 ccaattgtca tgctagaatt catgaacttg agttacctga cgataaagaa aaattgctga
     1381 agatcgttga aaaaagagaa aatagctttt ctaaaatacc gtaatttatt tttataggac
     1441 aaataagcta atgtgagcta tgccttctat ataagactaa gttctattat aagtaacgaa
     1501 gtaattctcg ttccttcctg tagtaatatt ttttaaataa cctataggct tcttcaaaag
     1561 tacatcctcc agtaaattct aagtactgga ggattctttt tctagtgttg ctttactcat
     1621 gaaaaagtag ttggctactt catcaaaatt tctgaacttt tttaatgtat aataaagagc
     1681 tcgatcattt actacaagga tgctagctcc tacattttca cagtttttta gcaagtaaat
     1741 ttttctaaaa tcacatcagg aaatgcatga gattctttaa gagaggcaat tatagggcta
     1801 tgattacggt tcgtgtaagt gacgccaaaa aagaatggct ttcttatatg gctgaatttt
     1861 atgggattac tttatctgaa cttctaaaaa actattcgat ggaacaacta gaagatgaat
     1921 acaatcgtca aacggctgaa attgcccata aacattggat aagaagtggg aaaacaacag
     1981 tattcatgga agaatcctta atgaatttgg tgggctggaa tgacctgccg tttaaaattc
     2041 acgcccgaaa ttcagaagca gctctgattg gcaaatacgt gtattggtag agatagaaga
     2101 taatcaattc gttgttatcg ctattcaagt agggcaccga agaaatgtct atgattaaga
     2161 gactgtttat ttttacaaaa aaataataat tggaatctag ttaaaactct tgaagtgaga
     2221 gatttgacta gattttttta tgaataaaat aaggacaaaa ttgtttagga agaatcatga
     2281 gtgtaaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
     2341 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
     2401 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
     2461 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
     2521 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
     2581 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
     2641 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
     2701 catatcccat tttgcgagca tatctgctat tactgtgact ttaataaagt ttttttagaa
     2761 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgagatt aatgctggaa
     2821 agtcatccta cagcgaattt ggaaacactt tatgtaggag gcggaacacc aacttcttta
     2881 tctgctaaac aacttgatcg actattatca ggtgctagag agatcctgcc ttttcaaaat
     2941 ggtaatgagt ttactgtaga agcgaatcct ggtgatttga cccgtgaaaa acttcaagtg
     3001 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga caatcgctta
     3061 ttgaaaaaaa ttggacgtaa acatactgca gaagatgtgt atcaaacaat gcaattttta
     3121 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
     3181 ctagaaggat atcgggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
     3241 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
     3301 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
     3361 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
     3421 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
     3481 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
     3541 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
     3601 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
     3661 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
     3721 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacttttttg
     3781 gggaacaatg tgtttgagcg ctttttattt gaaaaatagt ttgaagaata tttgtttttt
     3841 atcgaaactt gaaaagaaaa agtttagaaa gtttgacaat gaggtattga agtattgata
     3901 taactggatt tacatcaatt atttagaatt agcactcttg tgtttagagt gctaaaaaat
     3961 ggagtctttc tcttgacagg agaagggctt cttgctatat ttataagtga attagcactt
     4021 agaatatatg agtgctaatg agaggtgagg aagatgttaa caaagcgaca aaacgacatt
     4081 ttacgtctga tcatccaaca ctatacgagt agtggcgtgc ccgttggttc caaaacatta
     4141 atgtctgagg gagtcaaggc cagctcagca acgattcgca atgatatgaa agcattagaa
     4201 gatgaaggat tgctgttgaa gactcactct tcttctgggc ggattccatc tgctttaggc
     4261 tatcgttatt acgtggatca tttgttaaaa cctgctcgcg tagcagaaga tgatttgctg
     4321 ctgattcgtc agtctctagg cagagaattt catgaaatca atgagattat taagcaatct
     4381 gctgagatat tatctgagct gacaagctat acgacttttt cattgggacc tgaaataaaa
     4441 gaccgccgac tgactggctt tcgtatcgta cccttgaata atcgacagat tattgctatc
     4501 gtcgtaacag atatggggaa tgtagaaagc caagtgttca ctcttcccga atatatggga
     4561 agtcaggaac tagaaaaaat ggtacagatt gtaaacgacc gtctagtcgg agatcctctg
     4621 atgacggtgt accataaact tcgtacggag attccgatga ttcttcataa atatttccag
     4681 acgacagaag ggatgtctca gttatttgac accgttcttg gtcatgtttt tgaagaaaag
     4741 gtttatgtca gtggacaaat gaatctcttg gattatgagc cccgtcaaga tcttgatcag
     4801 ttcaaatcga tgttttcttt tatgaaagac tcagacggac ttacacaaat gatcactccg
     4861 atggatagca atattcatat caagattggt gctgaattag gcaatgattt attgcagaat
     4921 atgagtatga ttcaagcaag ttacgagata gatggtcatg gaagaggtac aatagccttg
     4981 ttagggccta ctagtatgcc ttattctcgg atgttcggtc tagtcgatgt ttatcgaaga
     5041 gaactagcag ctaaacttgc tgattactac cgctcgcttg acttgtcaaa ctcgtaatgt
     5101 gaataacgaa gctatttgca gctgatcgtt ctctctttct tagtaagagc aatcaaacag
     5161 ctgactgaat aatgaaagga agactgtatc gtgaaagaaa atcaagaaga actagataaa
     5221 gaaatgacag atgctcagcc agaaccggaa atcgatgtcg aagctgcaga agattcgggt
     5281 atttcagaag ctgaagcgga agaattcgaa acagcgaaat taaaagctga gttagaagaa
     5341 atggaagata aatacttgcg ggctcgtgct gaaatcgcaa atatggcaaa tcgaggaaaa
     5401 aatgagcgtg agcaattaca aaaataccgt tcacaagatt tagccaaaaa attacttcca
     5461 tcaattgaca acctggaacg tgcattagcc acagaagtgt cagatgatca aggggcaggt
     5521 ttgaaaaaag gcgtggaaat ggtcttggaa agtttgcgta acgcactgga agaagaagga
     5581 attgaaaaaa tccctgctaa aggagaagcc tttgatccta atcttcatca agcagtacaa
     5641 actgttccag cgactgaaga tacaccagcc gatacgattg tagaagtact acaagaaggc
     5701 tacaaattac atgatcgcgt gttaagacca actatggtca ttgttgcaca ataattgtgc
     5761 ggatataaaa cttacacttt caattaaaga aggagacgtt aattatgagt aaaattatcg
     5821 gtattgactt aggtacgact aactctgcag tagcagtatt ggaaggaaac gaagcaaaaa
     5881 tcatcgctaa cccagaagga aaccgtacaa caccttctgt tgtatcattc aaaaatggag
     5941 aaatccaagt aggggaagtt gcaaaacgtc aagctgtgac aaacccaaat acgatttcat
     6001 caatcaaacg ccatatcggt gaagcaggtt acaaagtaga tgtagaaggt aaatcatata
     6061 caccacaaga aatctcagca atgatccttc aatatatcaa aggattcgca gaagactatt
     6121 taggtgaaaa agtcgaaaaa gcagttatca ctgtgccagc ttactttaat gatgcacaac
     6181 gtcaagcaac aaaagatgct ggtaagatcg ctggtctaga agtagaacgt atcgtcaatg
     6241 aaccaactgc agctgctctt gcctatggtt tagataaaac agatcgcgac gaaaaaatct
     6301 tagtattcga ccttggtggc ggtacttttg acgtatctat ccttgaacta ggagacggtg
     6361 tctttgatgt attgtcaaca gctggcgaca accatctagg tggggacgac tttgataaca
     6421 aaatcatcga ctacatggta gcagaattca aaaaagaaaa cagcattgac ttgtctcaag
     6481 acaaaatggc tttacaacgc ttgaaagacg ctgctgaaaa agcgaaaaaa gatttatctg
     6541 gcgtatcaag cacacaaatc agcttgccat ttatcacagc aggagaatct ggaccattgc
     6601 atttggaaat gacattgaca cgtgcaaaat ttgatgaatt aacagctgac ttagtagaac
     6661 gtacaaaagt accagtacgt caagcgttaa aagatgcagg tctgtcacaa tcagaaatcg
     6721 acgaagttat tttagtcggt ggatctactc gtatccctgc agttgtagaa gcagtacgta
     6781 aagaaacagg aaaagaacca aacaaatcag ttaaccctga tgaagtagtt gctatgggtg
     6841 ctgctatcca aggtggcgtg atcactggtg atgtgaaaga tgttgtttta cttgacgtta
     6901 caccattgtc attaggtatc gaaacaatgg gtggagtgtt cactaaattg attgaccgca
     6961 atacaacgat tccaacaagt aaatcacaag tattctcaac tgcggcagat aatcaaccag
     7021 cagtagacat ccatgtatta caaggtgaac gcccaatggc tgctgacaac aaaactctag
     7081 gaagattcca attgacagat attccagcag ctcctcgtgg tattccacaa atcgaagtaa
     7141 cattcgatat tgataaaaac gggattgtaa atgtatctgc gaaagatcta ggtacacaaa
     7201 aagaacaaaa aatcacgatc aaatcttctt caggtctaac agacgaagaa atcgaacgta
     7261 tggtgaaaga tgcagaagca aacgctgaag cagataaagc tcgtaaagag gaagttgacc
     7321 tacgtaatga tgtagacgca ttgctattct ctgtggacaa aacattgaaa gaattagaag
     7381 gcaaagtgga cgaagaagaa gtgaaaaaag cagaaacagc tcgtgacgaa ttaaaagccg
     7441 ctgttgaagc aaacaacatc gaggaaatga aaactaaacg cgatgcattg aacgaaatcg
     7501 ttcaaaactt gactgttaaa ctatacgaac aagctgcaca acaacaagct caagaaaatc
     7561 cagaagcagc tcaaggcgga gcagatgatg tcgttgatgc tgattttgaa gaagtcgatg
     7621 gcgacgataa ataatcagtt cactgaattt cgagagaagg ccaaagttgc tactttggct
     7681 ttttcttttg aaaaatagtt tgaagtatcg gtttttgttt caaacaaaat atgatatgat
     7741 aacaagcgac gtgaaaatga tgagaaccaa taggaggaaa gcctatggca acaaaacgtg
     7801 attattatga agtcttaggg ctctcaaaag gagcatcaga agatgaaata aagaaagctt
     7861 atcgtaagct ttcgaaaaaa taccatcctg acatcaataa agaacctgat gcggaagaaa
     7921 aattcaaaga agtatcagaa gcatatgaga ttttaagtga cccgcaaaaa cgcgctgcat
     7981 acgaccaata cgggcatgca ggcacagatc caaattacgg tgctggtgga gctggtggcg
     8041 gatttggtgg attcggcggc ttctctggtg gcggcttcgg tggttttgaa gatatcttcg
     8101 actctttctt tggaggaggc ggtcggtcag tcgatccaaa tgcgcctcgt caaggtgctg
     8161 atttgcaata tacggtcgac ttgtcttttg aagaagcgat ttttggtgta gaaaaagaaa
     8221 tcaagtataa tcgggaagaa atctgtcata cttgtggtgg aaacggtgca aaaccaggca
     8281 cacaaccaac tacttgtcat aaatgtcatg gtgcaggtac gatcaatgta gaacgccaaa
     8341 cgccacttgg acgagtgatg agccgtcaaa cttgtgatgt ttgtcatggc acagggaaag
     8401 aaatcaaaga accttgtcca acctgtcatg gctcaggaca tgagaagaaa gcacattcag
     8461 ttaaagtgaa tgtaccagct ggtgtagaag aaggacaaca aatgcgttta gctggtcaag
     8521 gagaagctgg agaaaacggc ggaccatttg gtgatttgta tgtggttttt cgtgtagaag
     8581 aaagtgatat ctttgatcgt gaaggttctg aaatttacta tgaattgcct ttaagttttg
     8641 tacaagcagc tttgggggat gaagtccaag taccaactgt ccatggagat gtgaaattga
     8701 agatccctgc tggaacacag acaggtacga acttccgttt acgtggaaaa ggcgctccga
     8761 agttacgtgg tggtgcaaca ggcgaccaac atgtcaaagt aaaactgatc acaccgaaaa
     8821 atctaaatga acaacaaaga gaagcattac gtgcattcgc tgaagctggc ggcatcaaag
     8881 ttgaagaaca gcaagaagac ggtttcttcg ataagtttaa agatgctttt ggcgggaaaa
     8941 agcggaagta gtttttctag taaaaatata aagtagactt gcccaaattt tctatttcta
     9001 gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatacg ttcgcctggt
     9061 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata agataatcat
     9121 catagtttct gaaggtggta tcgactatgt acttgatatc gtcaatgaac cgatggtact
     9181 ttcttatgcg atggaacagg tagagcgatt gcttgtttgt gcaggagaac aagcgatgcg
     9241 gctggtacag ttaagtgaaa aaacaagaaa ctaaaaatgc ccggtcgtca tgaagcgacc
     9301 gggcattttt ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc
     9361 attttgcgga ttttcaaaga tttgttgagg cgttccgtct tctaaaaagt ttccgtcgtc
     9421 aatgaacatg acacgattgg ccacttcttt agcgaagccc atttcgtgcg taacgataac
     9481 catcgtcata ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc
     9541 ggggtcgagt gcagatgtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc
     9601 acgagcaata gccacacgct gcttttgtcc accggaaagc atttcaggat acatatcttt
     9661 tttatcttct agaccaactg tctgcagcaa tttgattgct tttttctctg cttcttctct
     9721 agattctttc ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg
     9781 gaataggtta aagtgctgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt
     9841 gcttttatcc atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa
     9901 acgattcaag caacgcaaaa atgtactttt tcctgaacct gaagggccaa tgatacagac
     9961 gacatctcct tcgttgatgg acacattgat gtcgtttaat actgtgttat cgccgtattt
    10021 tttgactaaa tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa
    10081 cttttgctaa ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt
    10141 aaacatacgt actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga
    10201 ctacaccgat tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga
    10261 atgatgggat catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat
    10321 aagaaagacc taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc
    10381 ctcgaacaat ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa
    10441 ggaagtcagg aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa
    10501 gaaccatcat tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag
    10561 cgcgtattgg tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa
    10621 aggagatcaa agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt
    10681 tttggataag tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg
    10741 aaccatccgc tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt
    10801 tcaatccttc attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg
    10861 catattttcc gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg
    10921 cataaccgat taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt
    10981 cgtacaattg atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt
    11041 cgcttaaaaa ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat
    11101 cttcataaga cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa
    11161 agtaagaatc agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa
    11221 tcattccgtc tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt
    11281 ccattcggat gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc
    11341 cttcgtactc gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa
    11401 ttacgtatac gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg
    11461 ccgttgtagt gttgtcagct tgtgcaacat gtccaaacgc aagtgtcaca agtcctgtca
    11521 ttaagaaaac aaatgagtga aataaatgct ttcttttcat aatcaacctc ctaaatttcc
    11581 aatattttat tattataggc gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa
    11641 tgaaaaaaat gcgagtttct aacattttat gttataaaac gtaacatgca taataagata
    11701 aaaaagtggc gaaagtatgt tcggtgtggt ataatttttt agtaaggaat gcctttgctt
    11761 tccttttctt tttattggtc gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat
    11821 gattgagaga gatacctccc gacactttga gcttgtctcg aaataccaac cggctggtga
    11881 tcagcctgaa gcgattaacc aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat
    11941 cttattaggt gcgactggaa cagggaaaac atatacgatt tccaatctta ttgagaaagt
    12001 gaataaacca acactaatta ttgcccataa caaaacattg gctggtcagc tgtacgggga
    12061 attcaaggaa tttttcccta acaatgctgt tgaatatttt gtttcttatt atgattatta
    12121 tcagcctgaa gcttatgtgc cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa
    12181 tgatgagatc gacaaattac gacattcggc tactagttca ttattagaaa gaaatgatgt
    12241 gatcgtcatt gcatccgttt catgtatttt tggattaggt tcgccttttg agtaccaaaa
    12301 acaagtagtt tcgattcgac agggagcaga actagatcgc aatcagttga ttcgtgactt
    12361 agtcagtatc caattcgaaa gaaatgatat agattttcag cgcggtcgtt ttcgagtgcg
    12421 cggtgatgtc gtggagattt ttccggcttc aagagatgaa cgtgccttac gtgtggagtt
    12481 ttttggagat gaggtcgaac gtatccgaga agtcaatgca ctgacaggtg aagttttagg
    12541 agaaacggaa cacgtggcaa tcttcccggc gactcacttt gtgacgaatg atgaacatat
    12601 ggagcatgcc gtagcgaata tcaaagcgga attagaacag cgtttaactg tcttgcgaaa
    12661 tgaaaacaaa cttctggaag ctcaacgttt agaacagcga accaattacg atattgaaat
    12721 gatgttagaa atgggctaca cgtcgggaat tgaaaattat tctcggcata tggacggacg
    12781 taaagaaggt gaaccgcctt atacgctatt ggatttcttt cctgaagatt ttttgatcgt
    12841 ggcggacgaa tcgcacgtaa ccatgcctca agtcagagga atgtataatg gtgaccgagc
    12901 gagaaaacag atgctagtag attacggttt ccgtttgcca tctgctttag acaatcggcc
    12961 acttcggtta gaagagtttg aaaaacatgt caatcagatc atttatgtct ctgcgacgcc
    13021 aggaccatat gaacatgaac aaacagatac cgttatccaa caaatcatcc gtccgacagg
    13081 gctgcttgac ccagtgatcg aagttcgtcc aatcatggga caaatcgatg atctagtagg
    13141 cgaaataaat gaacgagtcg aaaaagacca gcgggtgttt gtaacgacat tgacgaaaaa
    13201 aatggcagaa gacctcacag attatttcaa agaattaggg atcaaagtca aatatttgca
    13261 tagtgatatc aaaacgctgg aacgaacaga aatcattcga gatcttcgat taggtgaatt
    13321 tgatgtgtta gttggaatca atttgcttcg tgaaggatta gacgtacctg aagtttccct
    13381 tgttgctatc ttggatgcgg ataaagaagg ctttttacgt agtgaacgtt ccttagtcca
    13441 aacgattgga cgagctgctc ggaatgaaga aggtaaagta atcatgtacg cagacaaagt
    13501 aacagattct atgcgtctag cgatggatga aacatcaaga cgccgtacga ttcagcagaa
    13561 gtacaatgaa gaacatggga tcgttccaaa aacgatcatt aaagaaatcc gagatttgat
    13621 ttctattacg aaagaatcgg aagatgatac aaaagaagcg gtacaagtct cgtatgaaga
    13681 aatgacgaaa gaagaaaaag atacgttatt gatgaaatta gaaaaagaaa tgaaaaatgc
    13741 ggcgaaagca ttggattttg aaacagccgc taatgtgcga gatatgattt tagaattaaa
    13801 agcttcaaag taggaggaca acatggctaa cgataaaatt acgattcacg gtgcacgtgc
    13861 ccataacttg aaaaatatag atgtaacgat tccacgagat aaattcgtgg tcgttacagg
    13921 tttgtccggt tctggaaaaa gttccctagc atttgatacg ctgtacgctg aagggcagcg
    13981 gagatacgta gagagccttt ctgcttatgc tcgtcagttt ttaggacaaa tggataagcc
    14041 ggacgtagat agcatcgacg gattgagtcc ggcgatttct attgatcaga aaacaacaag
    14101 taaaaatcct cggtcgacag taggtactgt gacggaaatc aatgactatt tacgcttgtt
    14161 gtttgcacgt gtaggacatc cgatttgtcc caacgatcat atcgagatta ccagtcaatc
    14221 agtagatcaa atggcagatc aagtaatggc tttgcctgag cgcacaaaga tccaagtgtt
    14281 ggcaccgatc gtggtaaaga aaaaaggaca acataaaaaa atattcgaac gaatccaaaa
    14341 agagggatac gtccgcgttc gagtcgatgg tgagatctat gatttaagtg aagtgcctga
    14401 gctggagaaa aacaaaaaac atgatatcgc gatcgtcatc gaccggatca ttgtgaaaga
    14461 agggattcgt tctcgtctat ttgattcttt agaggcggct ctgcgattgg cagacggcta
    14521 tgctctggtt gacgtgatcg gtgaagaaga aatgttgttt agcgaacact atgcctgtcc
    14581 ttattgcgga tttactgttg gcgaactaga accaagactg ttctcattca atgcgccttt
    14641 tggagcttgt ccagactgtg atggtttagg tgtcaaatta gaagtcgatc aagatttagt
    14701 gattccagat agaacgaaga cacttagtca aggtgctatc gttccatgga atccaatcag
    14761 ctctcagtat tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta tcgatttaga
    14821 tacaccattc gaagaattac cggaagagca tcaggatatc atcttgaatg ggacagatga
    14881 tcttttccat ttccattatg aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt
    14941 cgaaggggta ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg
    15001 tgagcaaatg cgtctttata tgacagaatt aacttgtcag acatgtaaag gttatcgatt
    15061 gaatccccaa gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt
    15121 gtctattcaa aaagcagtag atttcttctc aaacttaacg ttatctgaac aagagaaagt
    15181 aattgccaaa ccgattttaa aagaagtaaa tgacagactt tcattcttgg aaaatgtggg
    15241 actagactat ctaactttga gtcgcgcttc cggaacatta tctgggggag aagcgcagcg
    15301 aatcagattg gctacacaaa ttggatcgaa tctttctgga gttttgtata ttttagatga
    15361 accatcgatc ggattgcatc aacgcgacaa cgatcgactg attggctcat taaagaaaat
    15421 gcgtgatctt ggcaacaccc tcgttgtagt cgaacatgat gaagatacga tgtgggcagc
    15481 agattatctg attgacgtcg gacctggggc cggagaacaa ggcggacaaa tcgtggctgc
    15541 aggcactcct gaagaagtag ctaatgatga aaattctttg acagggaaat atctttcagg
    15601 gaaaaaatca atccctgtac caaaagaacg ccgtaaaggt aatgggaaag caatcaagat
    15661 cactggtgct tcagaaaaca atctgaaaaa tattagtgtt gaatttccat taggagaatt
    15721 tgtagcagtc acaggtgtgt ctggttctgg aaagagtaca ttggtcaata gtatcttaaa
    15781 gaaatcgtta gcgcaaaaat taaataagaa ttctgctaag ccaggtaaat tcaagacaat
    15841 ttccggctac gaaagtatcg aaaagatcat cgatatcgat caaagcccaa tcggccggac
    15901 gccgagaagt aatccagcga cttatacaag tgtatttgat gatatccgtg ggttatttgc
    15961 tcaaacgaac gaggcaaaaa tgcggggtta taagaaaggg cgttttagtt tcaacgtaaa
    16021 aggcggtcgt tgtgaagctt gtcgcgggga tggaattatt aagatcgaaa tgcacttttt
    16081 gcctgatgtc tatgttcctt gtgaagtatg tcatggcaaa cgatataact ctgaaacatt
    16141 agaagtgcat tacaaaggaa aaagcattgc tgatattttg gaaatgacag tagaagatgc
    16201 tgtagaattc ttcaagcaca ttccaaagat tcatcgcaaa ctgcaaacga ttgttgatgt
    16261 tggcttaggt tatgtgacga tggggcaacc agcaacgaca ttgtccggtg gtgaggcaca
    16321 acggatgaaa cttgccagtg aattgcacaa aatctctaat ggaaagaatt tctatatact
    16381 agatgaacca acgacaggac ttcatagcga tgacatcgcc cgcttgttgc atgtattaca
    16441 aagattagta gatgctggta acacagtttt agtgattgaa cacaatctag atgtaatcaa
    16501 aacagcagat tatatcattg atttaggacc agaaggtgga gaaggtggag gaacgatcct
    16561 tgcgactgga acaccagaag aaatcattaa cgtaaaagaa agttatacag gtcactattt
    16621 gaaaaaaata atggtataaa gaggatagat agctgagata gttaaaaaaa cataaaaaaa
    16681 tgaatttttg cttgttttat taatatcagt gttctagaat cattacatgg tagttctttc
    16741 tacactgatt ggtatagaca aaaacttcct ataagttcaa tccgttcaaa aaagaattga
    16801 gcaatcggag gagtttcctt tgattgcttt tttctttatg gtatgatatt ccttgtgtag
    16861 ttggaaaaga aaaacaagag attatgagta agtgagagat aatgatgaac agtattgaaa
    16921 atgaagaatt tatccctata ttattaggaa gcgacatgaa cgtttatggt atggcacgtt
    16981 cttttaatga agcatacggc aaaatctgcc aagcatatgc ttcggatcag ttagccccta
    17041 cccgctacag taaaattgtc aatgtagaag ttattccagg atttgataaa gaccctgtgt
    17101 ttatcgagac gatgcttcgt ttggcaaaag aaagatattc tgataagagt aaaaaatatc
    17161 ttttgattgc ttgtggtgat ggatatgcag aattgatctc ccaacataaa caagagctgt
    17221 cagaatattt tatctgtcca tatattgatt attctttatt tgaaagactg atcaataaag
    17281 taagctttta tgaagtttgt gaagaatatg atcttccgta tcctaaaaca ttgatcgtca
    17341 gagaagaaat gttagtaaat ggacatttgg aacaggaatt gccttttgaa ttcccagttg
    17401 ctttgaaacc tgcaaacagt gtggagtatc tttctgtcca atttgaagga agaaaaaaag
    17461 cttttatttt agagactaga gaagagttcg atttaatttt aggccggata tacgaagccg
    17521 gttataaaag tgaaatgatt gtacaagatt ttatcccggg tgatgatagt aatatgcgtg
    17581 tgctaaatgc gtatgtagat gaagatcatc aagttcggat gatgtgtctt ggtcatccac
    17641 tgttagaaga tcctacacca gcatcgattg gtaattacgt tgtgatcatg ccagattata
    17701 acgaaaaaat ctatcaaacc attaaggcat ttttggaaaa aatcgaatat acaggctttg
    17761 ccaatttcga tatgaagtat gatcctagag atggagaata taaattattt gaaatcaatt
    17821 tgagacaagg tcgtagcagt ttctttgtga cattgaacgg cttgaatctg gctcgtttcg
    17881 tcacagaaga tcgagtgttt aacaaaccct ttgttgaaac gacctatgga acgaatcaat
    17941 cagataaagc aagattatgg atgggtgtgc ccaaaaaaat ctttttagaa tatgctcggg
    18001 aaaatgaaga caaaaaacta gctgagcaaa tgatcaaaga aaatcgttac ggtactactg
    18061 tgttttatga aaaagaccgg tcaatcaagc gctggttgtt gatgaaatat atgttccata
    18121 actatattcc aagattcaaa aagtattttc atgtgaaaga aggctagttc atggagaatt
    18181 ttttcagtat tttaggcgga atgggcacga tggcgacaga aagttttgtt cgcttgatca
    18241 accaccgaac gaaagctaca aaagatcaag aatatttgaa ttatgtctta ttcaatcatg
    18301 caacagttcc tgatcgtaca gcttatattt tagatcgatc agaagaaaat ccaatgccgt
    18361 ttctgctgga tgatattgag aaacaaaatt tattgcagcc gaacttcatt gtgttgactt
    18421 gtaacaccgc acactacttt tttgaagaat tacaagcggc aacggatatt cctatccttc
    18481 atatgccaag agaagcagca aatgaacttg tccgtcagca tacgacaggg agagtagcga
    18541 ttctaggaac agaaggaagt atgaaagctg gaatctatga acgcgaagtc aaaaatcttg
    18601 gattcgaaac agtgattcct gatactgttc ttcaagaaaa aatcaattat ctgatctatc
    18661 atgagatcaa ggaatcggat tatttgaatc aagagctata ttacgaaatc ttagaagaag
    18721 cggtagagcg cctaaactgt gaaaaagtta tcttaggttg cacagaattg tcattgatgc
    18781 atgaatttgc tgaagataac cattatccag tgatcgatgc ccaatctatt ttagcagatc
    18841 ggacgattga acgagcgcta gctgaaagaa gcaaggcatt ggatacagca tcggaaaagt
    18901 aaaaaaattg gatttggggc aaatatccta aaagatattt ggtccaaatc tttttgtaaa
    18961 agaaaaagaa aactggtaat gatacgcccg gaatgatata attttactat gcacaatgtg
    19021 agaggagttc gtaattatga cagataattt gcaattagtc atcataacag gtatgagcgg
    19081 tgcagggaaa acagtagcta tccaaagttt tgaagatatg ggctattttt gtatcgataa
    19141 tatgccgcct cgattgattc caaaattctg ggaattaatc aaagaatcag gcaaagtcac
    19201 aaaaatggct ttaggagtgg atttacgttc tcgttccttc tttgaagaaa tccaaaacat
    19261 gctgatcgaa atcgaaaata cgaacttcat cgacacttct atcttatttt tagatgcttc
    19321 agacgaagaa ctagtggctc gctacaaaga aacaagacga gcacatccaa tggcgatgga
    19381 cggtttgatc actgaaggga tacgtaaaga acgggcgata ctagacgact tgaaagccca
    19441 agcttccgtg atcattgata cgacgacatt gacaccacga caattacgtg aaaaaatcaa
    19501 tgagtcattc aaatcaaagg gagaccaagg atttcggatc gaatttgtat catttggttt
    19561 taaatacggg ttgccgatcg attcggatat tgtgatggat gtgcgttttt tgcccaaccc
    19621 tcattatatc ccggaactaa gaccattgac cggaaaagat cagccagtct atgattatgt
    19681 gatgtccttt gatgaaacgc aatcattcta ccataaattt ttggatcttc tggaaactgt
    19741 attgcctgga tacattaaag aaggaaaaag cagcttgaca gttgcaatcg gctgtacagg
    19801 cggtcaacac cgttctgtgg cattgactga acgtttggca aatgctttag ggaaaaacta
    19861 taaagtcaat gtgacacatc gggacaagga taaaaggaaa gagacggtga accggtcgtg
    19921 aaaatgaaaa cctaccggat aagaaaacca aagatcgtag tggtcggcgg tggcactggt
    19981 ctgccagtta tcctgaaaag tttgcgaaat caaggagcag atatcacagc agtagtgact
    20041 gttgcagatg atgggggaag cagcggtgca atcagagaat cgatcgctat ggctcctcct
    20101 ggtgacttga gaaatgtctt agtggcgtta tcggatatgc cacaattcta tgaagatatt
    20161 ttccaatacc gtttcaaaaa ggaagatcag tttttagcca atcatacgat cggtaatctg
    20221 atcattgccg cagtttccga gatgcgggga agcacttatg aggcgattca attattagca
    20281 aaaatgatgc atgtagatgg acatgtttat ccttcttcag aaactccttt gaccttgcat
    20341 gctgtcttta aagacggaac gactgcagta ggagaatcta aaatcgcgat cgaccgtaaa
    20401 accattgacc gtgtgtatgt ctgcaacaca aatgataaaa gtgaagccaa agcggctcga
    20461 aaagtcgttt cagcaattat ggacgccgac atggtcgttt tgggacccgg aagtttattc
    20521 accagtatcc ttccaaattt agtgatacca gaaatcggcg aagcaatgct caagacaact
    20581 gctgaaaccg tctatatctg taatatcatg acccaaaaag gggaaacgga acatttcaca
    20641 gatgccgatc atatacgtgt tttacacaag catttgaatc agccttttat cgatacagta
    20701 ttggtcaata cggaaaaagt gcctgaagat tatatggacc cagagatcta tgatgagtat
    20761 ctagtacaag tcgcacacga cttcaacgga ttgagaaatg aaggatgtcg agtgatttcc
    20821 accgactttt tggaattgaa agatggtggt gtcttccacg atggagacaa agtagtagaa
    20881 gaattgttcc gtttagtttt cggttcaaaa tattaagcaa tttattgttg gagaaaggag
    20941 gagaatagga tgtcatttgc tgcagatgtg aagaaagaac taactggttt ggaagttcat
    21001 cgagaacatg cgaaagcgga attagctgct ttgctgcgaa tgaacggctc tttaggtatt
    21061 gtgaaccagc agtttatttt gaacgtccaa acagaaaatg ctgcaattgc cagacgtatc
    21121 tattctcttt tgaaagatca ttatcaggta cgaagtgagc tactcgttcg taaaaaaatg
    21181 aaattaaaaa agaacaatgt ctatatcgtc cggttgaagc aaggcacaaa agatgtgttg
    21241 atggatcttg atatcatgga tggtatgatg ttcaatggac acgtttcgga cgagattatg
    21301 ggaaattcgc aaaaaatgcg ttcttactta cgtggtgctt ttatggcctc aggttcagtc
    21361 aataatccgg aaacgagccg ttatcatttg gaaatatttt ctatttatga acagcataac
    21421 caagacattt gcgatatgtt aaattattac ggtctacatg ctcgaacgct tgaacggcgc
    21481 aatggctata tttcttattt aaaaggagca gaaaaaattg ctgatttctt aacattgatt
    21541 ggtgcaacca actcaatgct ccgatttgaa gatgttcgaa tcgtacggga tatgagaaat
    21601 tcggtcaatc gtctagtcaa ttgtgagaca gcaaatctga acaagacgat tgatgcggct
    21661 tctaaacaaa tcgaaaacat cgaattgatc gaagcaaagg tcggtctaca tgcccttcct
    21721 gaaaaacttc aggaaatcgc agaacttcgg cttcaacatc cagaggtcag cttgaaagaa
    21781 ttaggggaaa tgattccctc aggtgcgata tcaaaatcgg gaattaatca ccgaattcgc
    21841 aaaataaatg aatttgcgga aaaactgcgt gaagaagcag tttaatccaa agaaaaaact
    21901 gatacatgaa gcaggagatg cgaatgtatc agtttttttt aaaagttata cttgcttttc
    21961 ttccgtcaaa ctataattcc atctttactg gagtaacata atttggaaag gcttctattg
    22021 tttttaatac aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttgtac
    22081 ataaattttt gcttaatata gaagtcctga tatagataag atcaagtatt tatctataac
    22141 tggcctgatt aattaaaaaa ctggaaggaa ctgctcgatc atccttgaga actgctcttc
    22201 ttacttctgg tcaagattct cgtggttggt ggataaggat ggttgataag ttcattggat
    22261 tagctagtcg aaaacaacca ggtgtaatta atgtagaagt tttaacaagg ggatggagtg
    22321 atttattcag tgataatcat aagttcataa atagtaattg agatgtatgg ttggaatacg
    22381 ttatacctga acttgaacta ccaagatttg gtttagaatt tgagaatcga tgtttataaa
    22441 gggagggcaa aaatgcgttt ttttacaact tttactatca ttatgattgc agtacttttt
    22501 atttttctag atatagcaaa gcgaaataca gcatttttat tatatcgggt tttacttcga
    22561 gcaggtctca taacatttat atcgatcgtt ggattttttc tattcacagt tattgtattt
    22621 atttggagaa ctccagcacc accattgcca gagattacat atggagaatt tccttttaga
    22681 cttgagtatg aattgaatga agaattacat gtgatagaag acacgttaat agttgaattt
    22741 gatggatttg gaatgaacga aggcataggg agatatcgta gatggacaag tcgtttagcc
    22801 agcggagaag atttagtgtt gctgttagaa gttagtgata ataagcaaat attttatttt
    22861 ccaggacctg ctaattatta catgggagat aggttgaatg gttataatca tacttttcct
    22921 agtgcttctt tcattgaaag ggaaagaggc attactagaa gggatatttt acatgacaaa
    22981 gaattattag aacaatttgg tccgcttgat caaaatacaa taaatgaaga agaattgtta
    23041 aatcaatata atataaggct agtgaattgg gaaatatctg aaccgattgt taacaatttt
    23101 ggtgattaga aaacaattac gataaataga aacaagccaa atcatcagca agtaaaagct
    23161 gatgatttgg cctttcttat acgagtagta ggaattcata gcgaatatta ttacgatcag
    23221 tagatatttt gatattaaag gagtattacg aactagtatg tgagaatgta gaggtgattt
    23281 gcgaataatg agaaatttat caaatagaaa caaaatatta atcattattg ttgttatagc
    23341 tgtgtttcat ctgggaacga atgcggtttt aagtagaata atattgggtc ctaagccacc
    23401 aagacctgcg attacccgag gggagtttga ttttagatta gaatatgaag tagatgggga
    23461 aaggattgtg atagaagata caattgttgc attatttgat ggattttctg cagatgcagg
    23521 tagcatggca tggtatcgca cgtggcgttt gcatttggcg agtgacaaga gggcacaaca
    23581 attattgtta catgagttag atgatggtcg aaaaatatat tatcattttg gaggagctgg
    23641 attttatatg gggaagttag tagcagtgta actgtaacaa ggaaagattt gccaggtctt
    23701 ggcattcgaa actccatatt ttatatcgag cgtgggagag atacatctat tgggaacagg
    23761 aaagggttaa ctcttgaaga gttatataat gactttggaa tccgtcttgt tagttgggaa
    23821 cacaatccac caatagaaaa tcgctttgaa taatcgaaat ctattaatcc atttcatcag
    23881 ccagtgaatg ttgtaatgga acaatttggt ttagtgttca aaaccattga aatactaggc
    23941 tgtgatcagc aaaaatgtat ctgaataaaa aagagagcaa ctattaaatg agaggagtta
    24001 ggagttgtta gctttatacg aatcaaagtg gcaaatacag atgcgtatgc atttgtgttt
    24061 gccatcttgc gtttggtaga cgagataggt ataagctaaa gaaacaactt gtttaaaata
    24121 aacgacctag ctcaacaaac gaccatctaa tcaactaaca gcatacataa aagcaactat
    24181 ttttgaagta gaattgaaat cgggataact taaaaagtga ttgggatact agtgtgaatc
    24241 accgtacata aatattactc gttaagggaa tttgagaaat tttgtatgaa aatttaagaa
    24301 ttagttagta tgcgcaagtc aaaaatatga ataaattatt tggtggagat aatttgctca
    24361 gcttgtcata agaaatgaga taatctggca gatgcaagct tttggacacc atttattcgg
    24421 aataaggagg ctgggacaga agcgtttaac tccaagaaat aagaaggagt tgcttctgct
    24481 tccgccgttt ataggtgttt agagcgtgag acaaaagtgt tctttacttt tgtcctacgc
    24541 tccttatttt tttattttgt ttggatcaat tcttttaatt cctctattga aatagattca
    24601 tcaaagattt ctttacccaa gatgacagtc ggaacaaatc gaatatgtgc ttgctcagct
    24661 tcgttgatga cagcttggct cagctgttcg tctttttgtt ctgtcagctt tagtttatct
    24721 actgcaaaat ccgctacttc ttggagagaa agatgtttcc actcgtcttg agtatcgaat
    24781 atttgtttga tctctttgat cgctttttga ccatcgctga ttgtgagata ccggtgcatc
    24841 acgtttcctc gctgcaagct ttctttttct ttatcgaaca atttgatcac tcgctgcaat
    24901 tgcccgcttt ggactgcttc ttctaatagc tcataagatt cttcaaacca ttgtcggcaa
    24961 tacggacaag ataggttgat gaattcgacc atttttttag gagcattcga tgaaccataa
    25021 aggattcctt tttgtgtatt tgttttcgtt gcatcaatca ctgatatatc catgttaaaa
    25081 cacctctttc gcttatagtt tatacatgtc tgacaaaaaa aggcaacgat ttagactgtt
    25141 tgtctaaaaa ttagatgacc aattcaaata gacatatgtg aaatattttt ataaataaaa
    25201 gaaaaaatgg aatgttttac gttataatgg aacagataat aagagagaat atgaacaagg
    25261 atgatgaatc atgcgaaaaa tgaaaacaat ggatggcaat acggcagccg catatatttc
    25321 ttatgcattt accgagattg ctgcaatata tccaataaca cccagttcga ccatggctga
    25381 attagtagat caatggtcag cagcaggaaa agaaaatgta ttcggtcaac cagtaaaagt
    25441 agtagaaatg caatccgaag caggagcagc tggtgtggtc catggatcat tgaaaactgg
    25501 tgcactgaca actacatata ccgcttctca agggctgctt ttgatgatac cgaatatgta
    25561 caagattgct ggggaactac tgccaagtgt tttccatgtt gctagtcggg cagtgacgac
    25621 aaatgcgttg aatattttcg gagatcacgg agacgtgatg gctgcaagac aaacaggatt
    25681 tgctatgtta gctgaaagca gtgtacaaga agtgatggat ttagcacctg ttgcccatct
    25741 tgcttctatt gaaggagaag tacctttttt gaatttcttt gatggattcc gtacgagtca
    25801 cgagattcaa aaaatcgaag ttttggaata tgaagaatta gctcctctga tcaatcagga
    25861 gaaattagct tcatttcgta aacgagggat gaatcccaat catccgactg ttagtggtac
    25921 gaaccagaat ccagacatcc attttcaaca acgggaaacg atcaacccct attatgataa
    25981 attacctgaa atcgttcaaa aatatatgaa agagatcaac cgattacgag gaacttctta
    26041 tgacttggtg acttattacg gagcagacga tgcggaagaa gtcattgttt ctatgggatc
    26101 agttgcacag acgattgaac aaacgattga ttacttgaat gcaaacggac ggaaaacagg
    26161 ttttttgaat atccatcttt atcgtccttt tccagtggaa acattcgtgc aaaagttgcc
    26221 tcatacagta aaaacaattg ctgttcttga tcgaagtaaa gaaccagggg caggtggaga
    26281 acctctctta ttagacgttc aaagtgcgtt atatgattct gaacttcgcc cggctgttat
    26341 tggtggtcgc tacggattgg ggtctaaaga tgtgactccc gaccaaatct cagcagtttt
    26401 tgatgaattg aaaaaagacc caagcatacg caaaaaacgg tttaccatag ggatcgtgga
    26461 tgatgtgact tatcaatctt tggaaccaaa ggaaagtctg gatttgacag aaccgcaaac
    26521 attccaggcc aagttttggg gatttggatc tgacggaaca gttggagcaa ataaatcagc
    26581 tatcaagatt attggagatc acaccgataa atatgcgcaa ggctattttt attatgattc
    26641 gaaaaaatct ggcggtctga ctgtctctca tcttcgtttt ggagatacgc cgatacgttc
    26701 agcgtatctt gtcgagcatg cggaccttgt tgcctgtcac acaccagctt acctgcatag
    26761 ctatgacttg gtcaaaggat tgaagccagg tggcattttt ttattgaata ctctttggag
    26821 cgacgaacaa ttagaaacgc atctgccctt aaaattaaaa cgttacttgg ctgaaaataa
    26881 cattagattc tatacgatca atgccatgag attggcccaa gaagtcggtt tgggacgccg
    26941 gatcaatacg gcaatggaaa cggctttttt taaattagct gacatcattc cttttgatga
    27001 ggtgcttccg ctgctaaaag aagaagccct gaaaagctat ggacataaat caatggctat
    27061 cgtagaaaaa aacatacaag caatcgatcg gacgaccgaa ctgcttcatc aagtcgcgat
    27121 cccagaccat tggagaacgt tggagatgcc tgaaaaaatc aataaaagga acactggtta
    27181 tatacaagaa atacttgaac cagtgaatgc acaggaagga aatcaactct ctgttggtac
    27241 acttgtagca aatggaatga caactggcga aatgccttta ggaacggctt ctatggaaaa
    27301 gcggggcgtc gctttagaag tacctgagtg gattagtgat cgctgtacga tgtgtaatga
    27361 atgtgccttt gtttgtcctc atgctgccat ccgtccattt ttagcagatg aagaggaaat
    27421 ggaagaagcg ccagaaggat ttatcgtgag agaaatgcga ggcgcagacg gcgtaaaata
    27481 ccgtatccaa gtttcagtgg aagactgtac aggttgtggt ctgtgtgtag aggcttgtcc
    27541 cgcaaaagaa aaagctttag tgatgaagcc ttatgaagaa cagaaagaac aagctgtgaa
    27601 ttgggctttt gcaatgacat taagacaaaa agcaaatcca gtcaaaggaa agaatactgt
    27661 cttgtctaca caattcaatc aaccgcttct tgaattctcg ggcgcttgct caggctgtgg
    27721 cgaaacacct tatgtaaaat tattgacaca aatgttcggt gatcgaatga tgatcgcgaa
    27781 tgcgacaggc tgttcttcta tttggggcgg tgcttctcct gcttctcctt atacgaccaa
    27841 tgaatgtggt caagggcctg catggtcaaa ttctttattg gaagataatg ctgaatttgg
    27901 ttatggtatg cttctagcgg cacagactcg tcgcgaggct ttggcaagtt caatcaaaga
    27961 agtattgcca aaagtatcag aagaactacg agaattgatg gaagactggt tggtacacat
    28021 gtttgagagc gaaggaacgc agcaaagagc agcaaaactg caagctgcga tcgaaagcga
    28081 acgatcaatt gtccctgaac tggaggatat atacaagaga aaggatctat tcgtaaaaaa
    28141 cagccaatgg atgattggtg gagacggatg ggcttacgac attggttttg gcgggatcga
    28201 tcatgtactt gccagcggag cagacgtgaa tattcttgtt ttggataacg aagtctattc
    28261 caatactggc ggtcaaacct caaaagcaac accagcttcg gcaatcgcta aattttcggc
    28321 tagtggaaag tatgcttcaa agaaagatct aggaatgatg gcgatgacat atggaaatgt
    28381 ttatgtggcg caaattgctt caggtgccaa tcaaatgcag acgataaagg cttttgaaga
    28441 agcagaaaag tatcctggac cttctttaat cattgcctat acgccttgta tcacacatgg
    28501 tttgcttggt ggaatgaaag aatcgcttaa agaagcaaaa gaagctgttt cttctggcta
    28561 ttggtcatta tatcgatata accctgcaat ggtcgaaaaa aacaagaacc caatgacttt
    28621 ggactacaaa aaacctgatt tctctgctat gcctgatttc atgaggaaac aagttcgctt
    28681 ttcctcgcta gagaacgcaa atccaaatct tgcaaaagct ttatttgaaa agactatcca
    28741 agacgctaaa acacgttttt acaactatgc tgcactgacg ggagaattag aaaaaatcaa
    28801 gaaacgactt gaaccagagg aatctatcga tgtacccgtt aagactagaa aagaaaaaac
    28861 gacggacctt gaagcagaag aaagaagagc tgccagagca gaacgacgta aaaaaatgga
    28921 acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat aattctctga
    28981 agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt tcctgaaaat
    29041 tatgcgtttt tctcaagaaa aatacgagca gatcagcctc taaaattaaa acatatgata
    29101 tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca aatcggggaa
    29161 ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc tctgaatttt
    29221 ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact gatccaactg
    29281 ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat tgttatccga
    29341 atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca agtcattacg
    29401 tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg actggaacat
    29461 cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga tgagaagatg
    29521 atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg tgcgctaatt
    29581 actatcgaac ggcatactgg attagatgag tatattgaga caggaattgc attagatgca
    29641 gatatcactg gagagctgct gatcaatatc tttattccta atacaccgct gcatgacggt
    29701 gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc tctatcagaa
    29761 agtatgttga ttccaaaaga attcgggact cgacaccggg cagctgtcgg tataagcgaa
    29821 gtcagcgacg cgattaccat cgttgtctct gaagaaactg gggatgtcag cattacatta
    29881 gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatttt gcgtagagaa
    29941 ctgattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt cctagacggt
    30001 atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc attctttaca
    30061 gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc cagtccgttc
    30121 caaatacgct ttctggaaat gaagcgtata atcaaacagt tactggggtg ccaatcaaga
    30181 tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg actgtcaaac
    30241 tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca cggatgttcc
    30301 gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaggttccg ctaaaaattc
    30361 aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca gtaacggttg
    30421 agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg ttcacaccat
    30481 ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc actacaggtg
    30541 acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg atggtcacag
    30601 atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg gaagtattaa
    30661 gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag ccaagcaagt
    30721 cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa agctatgatt
    30781 tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt gcatcgatcg
    30841 gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca actcgtaaaa
    30901 ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt gtggagatta
    30961 cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg acacaagaaa
    31021 atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca tcgacaacaa
    31081 ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca gaagaaacat
    31141 cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat taagttaaag
    31201 gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg gtgaagccaa
    31261 caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt atgtcctaag
    31321 ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata caagaatctc
    31381 aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta tcgaagtgtt
    31441 ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc aaaaagcaag
    31501 cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa tcaaattttt
    31561 cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag cattattaga
    31621 tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg tagaagaatt
    31681 tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg gtgacctttc
    31741 cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg ttaatcgttt
    31801 gtttgccgat cttgaaacag atttttatac catgggcaca aatccagacg ggttaaatat
    31861 caatgatggc gtaggctcta cccatccaga acgtttagct gagatggtcg ttgaaaaagg
    31921 agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg tggatgaact
    31981 tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact tggcagaaaa
    32041 aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag gattccacaa
    32101 agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc gttacgttgt
    32161 agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc acatgatttt
    32221 cttagatttt aacacaactg gagatgggat gctttcagga attcagttac tgaatatcat
    32281 gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct atccgcaaaa
    32341 attagtgaat atccgagtaa gtgataaaaa cggtgcgatg gatgttccgg caatcaaaaa
    32401 agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag ttcgtccttc
    32461 cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga aagttaatta
    32521 ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt aagagatatt
    32581 ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt ccttgttctt
    32641 ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt cgaatccccg
    32701 taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta gagtagttag
    32761 ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc cctctgagac
    32821 aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc gagtatgcgc
    32881 accagtcact tcttcactga tgattccaat cactggtgtt ccttcttcga tcaaagcaat
    32941 cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac tgatttcttt
    33001 caatttcaaa gcagcttcaa gagaaacgtt gtagtcagcg ccacgtccaa tgtaaaaggc
    33061 atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt tttgggcgat
    33121 gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga atacaagaga
    33181 agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta cagctatttg
    33241 tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca atagcagtgt
    33301 aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa gtgaagggta
    33361 gccgagacgg ttgattttta ccagaacttg gcggctatct gctgtttcgc cactttgact
    33421 caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa attcactggc
    33481 taaatgaacc tcaacaggga tctgtgtcaa tttttccaga gtctgtttac ctgctaaccc
    33541 cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag acaatgtatc
    33601 gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt gagcgatacg
    33661 acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat aggtaccttt
    33721 ctcgatatct gaagaatcta cttgtgcttc gaatgaagaa cgttctacga tgtcacctgc
    33781 aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat cttcaatctc
    33841 aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca cattgaatcc
    33901 atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa catcaggtgc
    33961 tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag cttttaaaaa
    34021 cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga taatctcagt
    34081 atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt cttcaaagtt
    34141 ttcgattacg ccattgtgaa cgagcacgaa gcgtccactt tgggaagtat gtggatgggc
    34201 gttttcttca gaaggttgcc cgtgagttgc ccagcgtgta tgaccaatac ctatatttcc
    34261 tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg ctttgaccag
    34321 ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac ggtattctaa
    34381 tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca ttccaacaat
    34441 tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct tccagctgca
    34501 ggaaaatcct tttgttttat ctttgcagat attttttgtg gattttctgt agagtgcagt
    34561 ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg tttacacaga
    34621 cctggcgcta tcctaaaagg tataactatc ctcctctttt gttttttatc tggaaacatt
    34681 taaaatcata accttttgcg aatgttcttg tcaacaaaaa aagaataaaa aataaattgg
    34741 tatatatcaa atgctttgtc aaatgctctt tttatgtcaa aaaataatgg gtttttatct
    34801 atcatctttt tcaggagttc ggtacaatag aaagtaatcg gatagaggag gagtaaaaat
    34861 ggattcaaaa attgagatca tgacactggg aatgttaaaa aaacaattgt ccaaattcga
    34921 agcatcagct ggtgtatcag atgatacgaa aatattttta gatactggct gggatagcat
    34981 ccaagaaatc gcacctgatg ccttggaagt tgctgaagct cgtgagttta cggtagaaga
    35041 tgaattgaca aaagagtcgt tttccgggta tgcgcgggaa gaaaaagcag agcggttcga
    35101 cacttctgag cagtcagaaa ctgtgatcgt cataaaaaac ttgtattaga aaggagaaag
    35161 aatgaataag agacgttggg cagcagttct gattgctgca ggattatttg tggtttcgtt
    35221 agtttcagct agtttgacta agcctcagga aaacgatcaa attgagggat tgaacagttg
    35281 gttatatggg gatgaagaac tagcttcaaa tgtgttggaa gagggagaca gtacgcaaaa
    35341 aatcgtaaaa ttatcagtag atggaacgat tgctgcggga gggtcttcag gtttatttgc
    35401 ttctgaaggt tacaatcata aaaattttat ggaacaactg aaggcgattg aagaagatcc
    35461 aacagtcaaa ggtatctttt tagaagtaaa ttctcctggt ggcggtgtct acgaaagtgc
    35521 ggaaatcgct aagaaattgg atacgattcg taaagaacat gatatcccaa tgtatgtcag
    35581 catgaaaaac atggcagcta gcggtggcta ctatatttct gctcaagcag ataaaatctt
    35641 tgcaacagaa gaaacagtga ccggctcaat tggtgtgatc atgtcaggat tgaattattc
    35701 aggacttcta gaaaaactgg gtgtggaaga tacaacagtt aaaagcggag cgttgaaaga
    35761 tatgggttca ggaacgcgac cagagacaaa agaagaagaa gcggtgcttc aagcatatat
    35821 cgacagcgca tatagtcgtt ttgtagcaat cgtcagcgag ggacggaata aatctgaaga
    35881 agaagtcaaa aaaattgctg atggaaggat ttatgatggt gtccaagcaa aagaagtagg
    35941 tttagtcgat gaattgggat ttcctgaaga tgctttaaaa gctatgcgaa aggaacaaca
    36001 gcttgaagat gcggaactag tagaatattc ttccacgcca tctgggtttg ctagtacatg
    36061 gttaggaagt aaactagcag aattccaggg attgaagcct tctgagacta gtcagatctt
    36121 atccgttctt gagaatctag ggacagcgga atcaccaaaa gcaatgtatt attacggagg
    36181 tgagtaagca tgtcagagct ttcaaatgaa aataaggaac aagaaaaaga taagcaacag
    36241 cagcctgctg taggacctga ctccaacttt tcccaaaagg tactgagctc ttttgaaaga
    36301 gagcctttga cgcccgaaga aatcaaaaag aaacaacgta aatggaaaaa gtatacggac
    36361 acaagaaatg aaccgccaaa agcaaatgat tttcctaagt atttctatgc aggttttttt
    36421 atccgaatgt ttgctttctt ggtcgatctt ttgtgtatcg gagctattac gcgtttaaca
    36481 cttggtattg ccaaaaatct aggttggctt aatttttcag attcttatct gagtatctac
    36541 ggattcacag ccttacttat ttatctagcg tattttattt tattgacgaa attcaatcat
    36601 ggtcaaacga ttgggaaaat gatctttggt attcgagtca tcagctttga tgaagaagaa
    36661 cttagctggt caaccgtttt agttagagaa ggtgcttgcc ggttcattct aaaatttcct
    36721 ttgctgatgt tagggtatct tccagcaatc tttaatcaga aaaagcaaca tctaggcgat
    36781 tttttcacta atacaagtgt tgttacgatc aatctgatca aagcattcaa ccaggaagtc
    36841 aacagctgat gagcaataaa aagcagtaac agttgaaagc accttctaaa agtcagatct
    36901 aaaatctagc ttttagaggg tgcttcagtt atttttaatt gtcatgcttt ttttgttttg
    36961 ctactattga acatgagctg aacatgagca agaggaggac actgcgtgaa gccgattgat
    37021 tttccagaaa aatatgagaa tttaatgcga gtagcacaac aagcactagc aaaccagcaa
    37081 tatcagcagg caaaagaatt atttcaacga gcttacgagt tgaaagaaag ctttgaggct
    37141 aatagcctgc ttgttttttg tctttacgag ttggacgaga aaaaagaagc attgaagcaa
    37201 gcactcttgc atgaaaaaca gtatcttcaa aatgaggagt ttgctgaatt ttattttgac
    37261 ttgttaatca gtacacaaga ttttttatat gctagaaaat tgatagcaag tacagatttt
    37321 tatgaagctt tcgagcagcg ggtcattgaa aaaatccaat ttgcagaaga attatccgga
    37381 caaatggaga ggcagaagat caaggcactc cacaataaat cggaggaact tccttcgttg
    37441 gaacctgcac gtcagcttgc gtctattgat gagatcgaac agcttcctta tcatgagttt
    37501 atccaaacag cgagtaaact tattgtatcg ccagaggtcc atattttagc aagggcaaaa
    37561 ttactggaga cacttaggca attgaacgaa tgccatcctg ttttctattt gacgattgaa
    37621 gaaaagttag tcaaagtcat accaaaagat ctgccaaagc cgcagcagca ggcaagttac
    37681 cagcaattat gtgtattttc agatcgttac ggtaatgaag atgcgctgct tagttccgtg
    37741 ttaaatgaag aattcacgtt gcaatctgca attgtttatc ctgtctatga tacatacatc
    37801 gaagacccga agcgctggtt tcaactgact gtagaggcgt gtacaggaaa aacgatgtat
    37861 gaaggtacag aagatgaaaa acaagatttt ttaaaaaaaa gagaaaaaat tttgcagcaa
    37921 atgatcttct tccattagat tttatcagtg aaaaagataa aaatgttatg aaaaacaagg
    37981 attctatcca ttcaatatgt ttacaatttg aaagggttag tgtactattt aaaggtatgc
    38041 atgaaacgtt tttgatgtat aaaatgaatt tctaaactcg gagggaatta aatgactgca
    38101 acatttgaaa aaaaaggcac gaatgatggt gttttaaaat ttacgattgc gcaagacgaa
    38161 atccaaaaag gattaacaac tgcgtttaat aaagtgaaaa aaagcttgaa cgtacctgga
    38221 ttccgtaaag ggaaagtttc tcgtcaagta ttcaaccgta tgtacggtga agaagcttta
    38281 tatgaagatg cattgaatgc tgtattacca gaagcttatg aagctgctgt caaagaagct
    38341 ggcatcgatc ctgtttctca accaaaaatc gatgttgaaa gcatggaaaa aggtcaagac
    38401 tgggtaatca ctgctgaagt aacagtgaaa ccagaagtaa aattaggaca atataaagat
    38461 ctagttgttg aaaaacaaga ccgtgaagta acagatgctg acgtggatgc acgtatcaaa
    38521 cgtgaacaag aagcacaagc tgaattagtt atcaaagaag atgctgcagc tgaaaatggc
    38581 gatacagtag taatcgactt cgaaggcttt gtagatggcg aagcgtttga tggcggaaaa
    38641 ggcgaaaatt actcacttga attaggttca ggctcattca ttccaggttt tgaagatcaa
    38701 ttagttggtc ataaatcagg tgaaagtcta gatgtaaatg taactttccc agaagattat
    38761 caagcagaag atcttgctgg aaaagaagca gtcttcaaag tgacaatcca tgaagtaaaa
    38821 gcaaaagaag tgcctgaatt agatgacgaa ttcgcaaaag atgttgatga cgaagtatca
    38881 acattagacg aattgaaaga aaaatatcgc aaagaattag tagaaacaaa agaaaaagca
    38941 gcggacgatg caaaagatga agcagcgatc cgtatggctg ttgaaaacgc agaaatcgtt
    39001 gaattgccac acgtgatggt tcatgatgaa gtacatcgtt caatggacga attcttgaac
    39061 aacatgcaac gtcaaggtat ctctcctgaa atgtactatc aattaactgg ttctacagaa
    39121 gaagacttgc ataaacaatt cgaaggtgaa gcagaaatgc gcacaagaac aaatcttgtg
    39181 atcgaagcaa ttgcagctgc tgaaaatctt caagcaacag aagaagaaat caatgcagaa
    39241 atccaagaat tatcagaaac atacaacatg ccaatcgaac aaatcaaacg cgtgttgaca
    39301 gaagatatgc tggaacatga tgtgacaatg aaaaaagcaa tcgaattgat cacaggtaca
    39361 gcgacagaag cgaaagaata gaaatcaagt aataaatcat ttctgaagaa gctacttcta
    39421 gaaaattgtt tattcgaaat aagggactgt gacacgattt tgtcacagtt ccttattttt
    39481 tgttaagagt aaaaaatgca aaggtatagg tcagttgatc ctttgttaaa cactttttct
    39541 ttttactaaa ctatgttatg atagtaaaag actgtaaagt ctttgatagt ttcttgcata
    39601 ctttttgcaa gttaaggggt gaagaccatg tatgacaatc caagtagcaa tgagaccgta
    39661 cgctgttctt tttgcgggaa aacccaagaa gaagtgaaaa agatcgtagc tggtccaggg
    39721 gtctacatct gtaatgaatg tatcgaccta tgtaaagaaa tcattgatga agaattttat
    39781 gaagaagctg tacgcgaatt cactgaagtc cctaaaccac tagaaattct tgaagtattg
    39841 aataactacg tgattggaca agatcgcgca aaacgctcgt tagctgttgc cgtatacaat
    39901 cactataaac gtgtcaatca gcaagcacaa gaaaacacag atgatgttga actgcaaaaa
    39961 agcaatatct gcttgatcgg acctacaggg tctggtaaaa catttttagc acaaacatta
    40021 gcaaaaacat tgaatgtacc ttttgcaatc gcagatgcga caagtttgac agaagctggt
    40081 tatgtggggg aagatgtaga aaacattttg cttaaattgc ttcaatctgc tgattacaat
    40141 gtggaacgtg cagaaaaagg gatcatctat attgatgaga tcgataaaat tgcgcgtaaa
    40201 agcgaaaatg tatctattac tcgtgacgtt tccggcgaag gtgtccaaca agccttgctg
    40261 aaaattctag aaggaactgt tgcaagcgtg ccacctcagg gaggacgtaa acatccacat
    40321 caagaattta ttcagattga tacgacgaac atattgttca tcgtaggtgg tgcatttgat
    40381 ggcatcgaaa cgattgtgaa gaatcgtatg ggcgaaaaga cgattggatt tggtacacaa
    40441 aaccaaaaac tttctgaaga cgaaagtgtc atgcagcata tcattcctga agatttacta
    40501 aaattcggat tgatccctga gtttatcgga cgattacctg ttatggcagc tttagagaaa
    40561 ttgacaacag atgatctggt acggatcttg actgagccga aaaatgcgct agtcaaacaa
    40621 tatcaaaaac ttctttctct agataatacc gaacttgaat tcgaacctga agcgttgaga
    40681 gccattgcga aaaaagcaat cgaacgcaat accggtgctc gaggacttcg ttccatcatt
    40741 gaagaaatca tgatggatgt gatgtttgat atcccttcaa atgaaaacat cgaaaaagtg
    40801 attatcacaa aagaagcagc tgaattgtct ggtgagccaa cagtaatcta taaagattca
    40861 gagaaaaaag cgggttgatg aaaagaggct gccgtaaaag tcattttggc ttttgcgaca
    40921 gccgcttgct ttattcatca ccttgttaag gagagagacc atgaaagtcc atcaagctga
    40981 aattatcata agtgcagtgg caccaaaaca atatccagat acacaattgc cagaaatagc
    41041 tctggcagga cgatcaaatg tagggaaatc ttcttttatc aacacattga ttgatcgaaa
    41101 gaatttagcc cgtacttccg gtaagccagg aaagacccag acattgaatt tttatttgat
    41161 cgagaacgca ttacattttg tcgatgttcc gggatatggg tatgcaaaag tatccaaaac
    41221 tgagcgagca aaatggggaa agatgatcga gacatattta acgactagag accagctacg
    41281 tgcggtcgtt tctttagtag atttgcgtca taagccttct caagaagata tccagatgta
    41341 tcaatttttg aagtattatg atatccctgt aattgttgtt gccactaagg cagataagat
    41401 ccctcgtggt aaatggaaca agcatgagag cgaaatcaaa aaagcgttgg attttgatcg
    41461 caatgacacg tttattctct tttcttccgt tacaaaacag ggaaaagaag aagcgtgggc
    41521 agcaatcgaa caagcaattg aatcataaaa agaataaaaa agacttacca gtcaaactct
    41581 ttcataaaag tttggctggt aagtcttttt ttgagcttaa cttacttttg tttcttgtct
    41641 tttttctttt cattttgtgg ctgtggtttt agaaatcgat cgatctcttc ttttggtgca
    41701 actgtttttt ccggatcgac agcaaatgat tcgaaaagtt tatctaaatt atctgtacgt
    41761 tcaaatttca accccattgt aaacacctct ttgtttcttt ttacttagtc tatcatgtaa
    41821 tcgtccaaat tggtttctaa gacataaagt tttagttttt ctttatcgtt ggttataatt
    41881 aaattataaa aagattttaa agaggtgaga aaatgattgg cgaagataaa ttattgatga
    41941 ctatccagct cacagactta aatgaagaag aaaaaaaaca ggaaataagc gattgggcta
    42001 ttttgacacg tctgttgatc cagccaacat ttatccgctc tttcacacaa caagcggatc
    42061 cgtatgatct tcgaaagctt caagaaaaaa taatgctagc atcggtaaga gacgaaagtt
    42121 ggttagttgt aggaaatgac gagaacgaat gtagttttcg cttggaagat aatcaattat
    42181 tgataaaaaa tgtattgtct atctctgttt tcaaagaaaa gcaatttttg attcgagact
    42241 tcattcagaa aaaaatgatt gctcatgggg tgttcgccta tatgcgagct tatagcgagt
    42301 ttatctatca caatacaaaa cagatctctc aacggctatt gtttgagaaa aaggatgaaa
    42361 tcgaacattt acctaaaatg aaacaacaaa acggggaagt agtagtcgat tgcaaccaat
    42421 ttccaggata cgatcttttt taccaaggtt tatgtttcac ttcgtgttgg gaaatgtatt
    42481 atagtcgcta ttatcatcaa atcatcccta aaccgatttt tctcgacatc caacaagtag
    42541 aaaaagtgaa ggaattagaa aacgaagtca tatgtatcca gctttatcga gaccccttta
    42601 actggcaaaa gccaaacaat caaatgtttc aatcctattt tagggaccag cttgggtttg
    42661 atcatcttgc ttgggacaat ggtgtcggtt tattgaaacc gccattcgtt gaatacattt
    42721 acactgacca tacgatccaa tctgtccagt atcagaatgc acaaatgcaa cctgttccta
    42781 aaaagaatgc ttcatttttt gtaacaaaaa gttatgatat tcaacaagga gattataaag
    42841 aaaggcgtgt tcgcggaaca ctaaatgctc aggcgtattt tccttgggtt gacgagaatc
    42901 gctcgcgcat gatgtgctat aaagttatcg atccgactgt tgctttggat aatggaatcg
    42961 aagcttattg ttattatatt agggaatatc tagaagtaga ggtaacagat gaaaaatatc
    43021 aagagtatct tttatcctta agaatctatg ttccttcaga atccctgtca gaattaccgt
    43081 taaaggaaat caaacataga ctctctgatg ttaccttcaa acgaataaag aaaaagcggc
    43141 gaagtatcca attcgatgtc aagaaaggtg agcagcatct gcgtgtgcaa tttttagatt
    43201 accgagaatt agaacaattg attactttac aaaaaatatg aggtgaagaa aatggataaa
    43261 caactctatt atcgaataat ggttccggca tcattattat ccgtctgtct cttttttgtt
    43321 cctaattttc ctattcgatc agtcatcggt tttttggtca tgtttggcgg gatgggactt
    43381 tattattatc ttcttcataa ggaaagaaaa cctgcttctg tttatatgaa aaaaacaaga
    43441 agcgaaaata aaa
//
LOCUS       DAHHPA010000011        41389 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.011, whole genome shotgun sequence.
ACCESSION   DAHHPA010000011 DAHHPA010000000
VERSION     DAHHPA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.011"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            214..498
                     /locus_tag="NBH55_000566"
     CDS             214..498
                     /locus_tag="NBH55_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCD1508578.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="NBH55_000567"
     CDS             complement(779..1198)
                     /locus_tag="NBH55_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCD1508579.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="NBH55_000568"
     CDS             complement(1200..1784)
                     /locus_tag="NBH55_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCD1508580.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="NBH55_000569"
     CDS             complement(1831..3306)
                     /locus_tag="NBH55_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCD1508581.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="NBH55_000570"
     CDS             3487..3798
                     /locus_tag="NBH55_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508582.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="NBH55_000571"
     CDS             complement(3913..6249)
                     /locus_tag="NBH55_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1508583.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="NBH55_000572"
     CDS             6391..7059
                     /locus_tag="NBH55_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1508584.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="NBH55_000573"
     CDS             7062..8105
                     /locus_tag="NBH55_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD1508585.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="NBH55_000574"
     CDS             complement(8342..9223)
                     /locus_tag="NBH55_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1508586.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="NBH55_000575"
     CDS             9382..10110
                     /locus_tag="NBH55_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCD1508587.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="NBH55_000576"
     CDS             10266..10583
                     /locus_tag="NBH55_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCD1508588.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="NBH55_000577"
     CDS             10576..11184
                     /locus_tag="NBH55_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCD1508589.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="NBH55_000578"
     CDS             11239..12030
                     /locus_tag="NBH55_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCD1508590.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="NBH55_000579"
     CDS             12168..12656
                     /locus_tag="NBH55_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCD1508591.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="NBH55_000580"
     CDS             12707..13579
                     /locus_tag="NBH55_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCD1508592.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="NBH55_000581"
     CDS             13755..14117
                     /locus_tag="NBH55_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD1508593.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="NBH55_000582"
     CDS             14126..14878
                     /locus_tag="NBH55_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1508594.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="NBH55_000583"
     CDS             14892..16694
                     /locus_tag="NBH55_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508595.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="NBH55_000584"
     CDS             16971..18011
                     /locus_tag="NBH55_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCD1508596.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="NBH55_000585"
     CDS             complement(18063..20207)
                     /locus_tag="NBH55_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCD1508597.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="NBH55_000586"
     CDS             complement(20564..21937)
                     /locus_tag="NBH55_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1508598.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="NBH55_000587"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="NBH55_000587"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCD1508599.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="NBH55_000588"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="NBH55_000588"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCD1508600.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="NBH55_000589"
     CDS             complement(23385..24398)
                     /locus_tag="NBH55_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCD1508601.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="NBH55_000590"
     CDS             complement(24479..25078)
                     /locus_tag="NBH55_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1508602.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="NBH55_000591"
     CDS             25264..25965
                     /locus_tag="NBH55_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1508603.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="NBH55_000592"
     CDS             25975..28686
                     /locus_tag="NBH55_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508604.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="NBH55_000593"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="NBH55_000593"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCD1508605.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="NBH55_000594"
     CDS             complement(30825..32006)
                     /locus_tag="NBH55_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1508606.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="NBH55_000595"
     CDS             32258..33781
                     /locus_tag="NBH55_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCD1508607.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="NBH55_000596"
     CDS             complement(33850..34578)
                     /locus_tag="NBH55_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1508608.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34591..35871)
                     /locus_tag="NBH55_000597"
     CDS             complement(34591..35871)
                     /locus_tag="NBH55_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCD1508609.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36012..36614)
                     /locus_tag="NBH55_000598"
     CDS             complement(36012..36614)
                     /locus_tag="NBH55_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCD1508610.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36859..37065
                     /locus_tag="NBH55_000599"
     CDS             36859..37065
                     /locus_tag="NBH55_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508611.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37377..38096
                     /locus_tag="NBH55_000600"
     CDS             37377..38096
                     /locus_tag="NBH55_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD1508612.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38177..38860)
                     /locus_tag="NBH55_000601"
     CDS             complement(38177..38860)
                     /locus_tag="NBH55_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCD1508613.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39078..41264
                     /locus_tag="NBH55_000602"
     CDS             39078..41264
                     /locus_tag="NBH55_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCD1508614.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13426 a         7338 c         8305 g        12320 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga
    34621 aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc
    34681 atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt
    34741 ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac
    34801 ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc
    34861 ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt
    34921 atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat
    34981 catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact
    35041 tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata
    35101 agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt
    35161 ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata
    35221 atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct tattagagat
    35281 tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt
    35341 attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata
    35401 gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa
    35461 ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa
    35521 gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa
    35581 aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag
    35641 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    35701 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    35761 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    35821 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    35881 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    35941 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    36001 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    36061 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    36121 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    36181 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    36241 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    36301 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    36361 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    36421 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    36481 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    36541 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    36601 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    36661 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    36721 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    36781 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    36841 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    36901 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    36961 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    37021 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    37081 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    37141 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    37201 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    37261 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    37321 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    37381 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    37441 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    37501 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    37561 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    37621 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    37681 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    37741 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    37801 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    37861 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    37921 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    37981 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    38041 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    38101 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    38161 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    38221 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    38281 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    38341 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    38401 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    38461 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    38521 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    38581 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    38641 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    38701 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    38761 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    38821 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    38881 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    38941 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    39001 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    39061 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    39121 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    39181 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    39241 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    39301 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    39361 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    39421 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    39481 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    39541 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    39601 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    39661 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    39721 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    39781 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    39841 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    39901 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    39961 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    40021 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    40081 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    40141 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    40201 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    40261 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    40321 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    40381 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    40441 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    40501 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    40561 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    40621 aatgcgatca atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt
    40681 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    40741 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    40801 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    40861 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    40921 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    40981 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    41041 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    41101 aaccagtgac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    41161 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    41221 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg
    41281 acagaagtgg tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt
    41341 tgtgaaattt cagcttattt tcgaagaagc tatttctgga acacagttt
//
LOCUS       DAHHPA010000012        40064 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.013, whole genome shotgun sequence.
ACCESSION   DAHHPA010000012 DAHHPA010000000
VERSION     DAHHPA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.013"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            252..743
                     /gene="nrdR"
                     /locus_tag="NBH55_000603"
     CDS             252..743
                     /gene="nrdR"
                     /locus_tag="NBH55_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCD1508615.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            766..2139
                     /locus_tag="NBH55_000604"
     CDS             766..2139
                     /locus_tag="NBH55_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCD1508616.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            2139..3065
                     /gene="dnaI"
                     /locus_tag="NBH55_000605"
     CDS             2139..3065
                     /gene="dnaI"
                     /locus_tag="NBH55_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCD1508617.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            3194..3634
                     /locus_tag="NBH55_000606"
     CDS             3194..3634
                     /locus_tag="NBH55_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCD1508618.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3778..4479
                     /locus_tag="NBH55_000607"
     CDS             3778..4479
                     /locus_tag="NBH55_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCD1508619.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            4476..5597
                     /locus_tag="NBH55_000608"
     CDS             4476..5597
                     /locus_tag="NBH55_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCD1508620.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            5612..6844
                     /gene="pepT"
                     /locus_tag="NBH55_000609"
     CDS             5612..6844
                     /gene="pepT"
                     /locus_tag="NBH55_000609"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCD1508621.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            6976..7884
                     /locus_tag="NBH55_000610"
     CDS             6976..7884
                     /locus_tag="NBH55_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCD1508622.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7919..8191
                     /locus_tag="NBH55_000611"
     CDS             7919..8191
                     /locus_tag="NBH55_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCD1508623.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            8202..9248
                     /locus_tag="NBH55_000612"
     CDS             8202..9248
                     /locus_tag="NBH55_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCD1508624.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            9499..12108
                     /gene="clpB"
                     /locus_tag="NBH55_000613"
     CDS             9499..12108
                     /gene="clpB"
                     /locus_tag="NBH55_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCD1508625.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(12259..12945)
                     /locus_tag="NBH55_000614"
     CDS             complement(12259..12945)
                     /locus_tag="NBH55_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508626.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(12923..13417)
                     /locus_tag="NBH55_000615"
     CDS             complement(12923..13417)
                     /locus_tag="NBH55_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCD1508627.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(13437..14657)
                     /locus_tag="NBH55_000616"
     CDS             complement(13437..14657)
                     /locus_tag="NBH55_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1508628.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            14804..16639
                     /gene="lepA"
                     /locus_tag="NBH55_000617"
     CDS             14804..16639
                     /gene="lepA"
                     /locus_tag="NBH55_000617"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCD1508629.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            16835..17746
                     /locus_tag="NBH55_000618"
     CDS             16835..17746
                     /locus_tag="NBH55_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508630.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGQLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            18745..18996
                     /locus_tag="NBH55_000619"
     CDS             18745..18996
                     /locus_tag="NBH55_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508631.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            19079..19351
                     /locus_tag="NBH55_000620"
     CDS             19079..19351
                     /locus_tag="NBH55_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508632.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            19441..19668
                     /locus_tag="NBH55_000621"
                     /pseudo
     CDS             19441..19668
                     /locus_tag="NBH55_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19826..22126
                     /locus_tag="NBH55_000622"
     CDS             19826..22126
                     /locus_tag="NBH55_000622"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HCD1508633.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTATQPTVEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPVT
                     SQPVEETASITTPASETSTPEGEKAAEATPSVKPESSEKQAEEKQRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIEDQKKKEQDIPKTGTPTLLVTVP
                     TNRSVRVRRATNTAVLGGRKIDWGENIGVTSSPNNPNFTLNYMVRFEINGQVAFCIEP
                     GVSAAKGQVYTATALENYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLLNNFDVRATFHNQTHTIKIGETLKVKSGNDFLQRV
                     KGIQKPKGINVSIHGNEAWITVTKEAPEKAKIHFNQIEVLGTPLAYRRPGAQTIGILN
                     PFEPGDSVVNLTILKQGNIRVFKEDAETSVKAQGASSLNNAVYGLYQADGKKIKEVTL
                     KNVNGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLIDSMNPDNKPKLAGIEISLTSKTTGKVVKTVVTDKDGYATFGKNAV
                     VFDTYILSETKGKEGYKLFEPFEVTISENGQTFHYALEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTFTYGKDRYRLEE
                     IKAPVNYVLNQNHWYSRLPMIQNQSK"
     gene            22084..24108
                     /locus_tag="NBH55_000623"
     CDS             22084..24108
                     /locus_tag="NBH55_000623"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HCD1508634.1"
                     /translation="MVFSITNDTEPIKVIHFANRQARKNVKLFKYEEWNKKKTPLAGV
                     TFTLYNQAGKALGEYITDQNGEILVKGLVANNYYFIENTPLESFQPNTDKQAFTITFK
                     QNAPDDGETLLVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGK
                     EYTISGVLMNKATNTPILQDGKEIRKTLTFIADKVNGTKDLEFVVNASVLKGQTIVVF
                     EKLFRDGIEVAAHANINDENQSVRVNNPDVHTTAVFEDGLTVADPTGLFTLNDMVKIK
                     EIIPGKAYLVKGILMDKATGEALLVHGQPVESAGGFIAKAENETVIVPFTFDLTGLNG
                     REIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKVVNPLMKITI
                     TDAVKYHDLIVGKTYTVNGILMDKSTEKPVLVDDKEITSTLTFVAERIDGTVNLDFVL
                     DARALRGKEVVVFEDLFRDGVLLASHADITDKDQTIKIVNPTITTKATNKNGGKEILA
                     LPNQTVLEWVKVDGLAIGQLYKLLVQGYLTPDGTPFEGTHAEKVFTADKEMMEFLFEF
                     LVDGRNLAGKGLSFVEWLQAKTEDDKEAFEEVVKHNVDLTNKDQTVQFVEAPKPPQPV
                     KPEQPVKAATVVPMLPKTGEQTSSILMVLGLVVLVIVLGITLYKVRKAKD"
     gene            24207..24572
                     /locus_tag="NBH55_000624"
     CDS             24207..24572
                     /locus_tag="NBH55_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508635.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIEEVL"
     gene            24574..25026
                     /locus_tag="NBH55_000625"
     CDS             24574..25026
                     /locus_tag="NBH55_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508636.1"
                     /translation="MGLKFPNNYDITEALDETCVGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNLKQSQEKGLDQKQPSVDSKEKEKK"
     gene            25095..25472
                     /locus_tag="NBH55_000626"
                     /pseudo
     CDS             25095..25472
                     /locus_tag="NBH55_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25641..26951
                     /locus_tag="NBH55_000627"
     CDS             25641..26951
                     /locus_tag="NBH55_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCD1508637.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMRWSRLKEGYFASVYWRQLLA
                     RMLIDNRFIYTKKQVGEKQMKEKMRLPKVYFYQKKQELVISFPLDGGRFHERFLKMGN
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     LLISGAIGGGKTYLLYSLIKACLDVGTVDICDGKAADLAALGDVDIFKGHVFYKTNEE
                     MIACLRNALKEMNQRYAYMKTMKEPKYEPGKNYAYYGISPHFIFFDEWAAFYGSLDME
                     TRNKVDKLIQPLVLKGRQAGMYVILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            26974..27285
                     /locus_tag="NBH55_000628"
     CDS             26974..27285
                     /locus_tag="NBH55_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508638.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVGLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            27645..28901
                     /locus_tag="NBH55_000629"
     CDS             27645..28901
                     /locus_tag="NBH55_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCD1508639.1"
                     /translation="MTPIELRNYRKKLSAIHASGGGKKLLSQKRFAQLLGCSVDYIKK
                     MEQGKRKIPEDFEQRIKERFRLYVESGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDERWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQSYLEWMGTSGGKIA"
     gene            28898..29443
                     /locus_tag="NBH55_000630"
     CDS             28898..29443
                     /locus_tag="NBH55_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508640.1"
                     /translation="MNSYMTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLESIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLATVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            29444..29944
                     /locus_tag="NBH55_000631"
     CDS             29444..29944
                     /locus_tag="NBH55_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508641.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKRELEGQFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            30024..30581
                     /locus_tag="NBH55_000632"
     CDS             30024..30581
                     /locus_tag="NBH55_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508642.1"
                     /translation="MSLEFLGEPIEKEEFVLHYMDLFIQLIKKLCAIDEFYPREQDYL
                     AAERIKISLLWEKFTDENNRPPDYRYLSDCLTNNVLSKEYLFIKREKNQMSVEHFCDC
                     YIALLRSQQLLTVERYGAEFGYVMQAERHRATRCYEKEDQVIDGYEELCIRNNRYLQQ
                     RLYEQLMMDFSNLYRGYECSMIQER"
     gene            30601..30946
                     /locus_tag="NBH55_000633"
                     /pseudo
     CDS             30601..30946
                     /locus_tag="NBH55_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30965..31879
                     /locus_tag="NBH55_000634"
     CDS             30965..31879
                     /locus_tag="NBH55_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1508643.1"
                     /translation="MIKTTLAVVTTVGITLFSFQLGTLLVNADETVETPKSEIVDTLP
                     GEELPDVETELSGGASETPEQSQPEQPEKELIESEPEFPESTLPTDPIEEEGVHGSTT
                     TDSSTVTTPEQSGTTSSSEKTTDSSSTEQPTMSSTSEESKEEPSQPAEKASEKSEAIP
                     KENKPVVPAKEVTVSVTPSGEITTNTSQGTSVPIVTSNVEELTHIPTPTTPLKVESGQ
                     TIVGVKDGIPLVQDSEGNLVKDLSIPVKKLPSGNIEVKTADGKTKVLPKTGEEIHVVL
                     SVLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            31958..32950
                     /locus_tag="NBH55_000635"
     CDS             31958..32950
                     /locus_tag="NBH55_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1508644.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKGVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKIDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTLVITKKTNQYYVEKLTHNWLDTKGEERK"
     gene            32947..33165
                     /locus_tag="NBH55_000636"
     CDS             32947..33165
                     /locus_tag="NBH55_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508645.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNNPTRVFDAFGKIIEKLFMN"
     gene            33178..33588
                     /locus_tag="NBH55_000637"
     CDS             33178..33588
                     /locus_tag="NBH55_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508646.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVAI
                     FEYLFLRSFISWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYVV
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            33641..36169
                     /locus_tag="NBH55_000638"
     CDS             33641..36169
                     /locus_tag="NBH55_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1508647.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVLNLIWLDVAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGTSYLALLPIGETPENMRSCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATIIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSFVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVEYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILTHLRLEVNEANLSEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            36185..38395
                     /locus_tag="NBH55_000639"
     CDS             36185..38395
                     /locus_tag="NBH55_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508648.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYLLLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVINPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQSSKQQGNNQRKPLLNKGIAFVGQSIPLPAGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPKSSVATLSGNRSVQQLGQRSSESKPSISAP
                     KSLQPAFSSLDKSTDQSKKIEKDPKTLPILDAKLGTDFTLPKGRTVQKPLSKKG"
     gene            38403..39494
                     /locus_tag="NBH55_000640"
     CDS             38403..39494
                     /locus_tag="NBH55_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCD1508649.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEDGGEATGLAVAFNGE
                     YTEGLPIFSEIRGRGSITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDTFAIKHWNSLPTKK
                     SSLVAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YDWTAYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKMK"
     gene            39515..40057
                     /locus_tag="NBH55_000641"
     CDS             39515..40057
                     /locus_tag="NBH55_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508650.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
BASE COUNT        13571 a         6456 c         8592 g        11445 t
ORIGIN      
        1 tttttgtgct agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga
       61 tcagtattgg aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga
      121 aaaaattgtt cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg
      181 tgttataata caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga
      241 ggtgagacaa aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc
      301 gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc
      361 gattcactac atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg
      421 aacgcgaaga gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa
      481 gacctgtagc tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca
      541 gtctcggaga aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag
      601 ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata
      661 tgtctgtttt cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag
      721 agaataataa agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg
      781 aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac
      841 agcaagaacg ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc
      901 tatttcaccc tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat
      961 gcagatctgc ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag
     1021 ctagaaggaa tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt
     1081 tttatttatg agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg
     1141 gcttttcttc ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt
     1201 gaacctaaaa ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt
     1261 tatcgcttca atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa
     1321 tcttttgaag aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag
     1381 tttttgaatg attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa
     1441 caagtcgaca tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc
     1501 agtcaatctt atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt
     1561 ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac
     1621 agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa
     1681 gcacagagca ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat
     1741 gtttctaaac aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt
     1801 agcgtgatca atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat
     1861 gccagttatg taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa
     1921 gaagcgatcc agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag
     1981 caaacaaatt atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc
     2041 aaccagccaa aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt
     2101 tttaaagaat acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat
     2161 tgaagaggat catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag
     2221 tcttaaatga tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag
     2281 cggatattga aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc
     2341 aaaaaaacga tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc
     2401 attatgtgga tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag
     2461 aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg
     2521 aaaactttga acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag
     2581 agaaatatac tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg
     2641 gtattggaaa aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca
     2701 ccacgacgct agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag
     2761 atcaagtggc agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca
     2821 ttggtgcaga tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat
     2881 atcggatgca ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg
     2941 aacagcattt gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa
     3001 tggaacgcat acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg
     3061 gttgaacagt tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat
     3121 cattatatgg acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa
     3181 cggaggattt tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct
     3241 tttagcaggc tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg
     3301 tcaaacagta aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat
     3361 tgaaagtatt actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt
     3421 catggccaaa gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt
     3481 aaatgctact gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt
     3541 ccaatttaca ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg
     3601 agattcgatt attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga
     3661 aaatgttgtt ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat
     3721 tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg
     3781 aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct
     3841 cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat attaagagga
     3901 aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag
     3961 caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat
     4021 gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg
     4081 atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg
     4141 atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat
     4201 aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc
     4261 gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg
     4321 cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa
     4381 gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag
     4441 caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca
     4501 tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac
     4561 tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc
     4621 cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc
     4681 caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag
     4741 atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg
     4801 atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa
     4861 agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga
     4921 aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa
     4981 gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg
     5041 ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa
     5101 ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt
     5161 atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat
     5221 tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag
     5281 atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt
     5341 gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata
     5401 tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa
     5461 ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat
     5521 cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc
     5581 catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt
     5641 acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca
     5701 gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga
     5761 tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca
     5821 tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta atgcagtagg
     5881 agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga
     5941 agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac
     6001 attgatcaca acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga
     6061 aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa
     6121 agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca
     6181 attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga
     6241 aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac
     6301 ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc
     6361 agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt
     6421 ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa
     6481 attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga
     6541 tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga
     6601 aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc
     6661 agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc
     6721 gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt
     6781 acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag
     6841 ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct
     6901 gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta
     6961 aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat
     7021 gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta
     7081 tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca
     7141 gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc
     7201 gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact
     7261 gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg
     7321 atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc
     7381 tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa
     7441 cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa
     7501 aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg
     7561 acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc agcgacccta
     7621 gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt
     7681 tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca
     7741 gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac
     7801 acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca
     7861 ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat
     7921 ggatgtattg ttacacaatc ttttgtggtt ttaccttttc attgtgaatt tttacttatt
     7981 ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa
     8041 taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt
     8101 tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt
     8161 tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga
     8221 aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt
     8281 taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt
     8341 tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag
     8401 aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct
     8461 atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt
     8521 tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga
     8581 ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc
     8641 ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga
     8701 tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg
     8761 tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca
     8821 atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc
     8881 tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt
     8941 tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct
     9001 ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg
     9061 tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac
     9121 tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg
     9181 aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc
     9241 aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat
     9301 aagataattt tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt
     9361 taagatttat ataagtcgag aaaaaaagtc aaaaaaccat gtgtatattt tgaccaaaat
     9421 tgaccaaagc gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa
     9481 ttaggagggt atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt
     9541 gctgaagctc aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta
     9601 tggaaaatct ttttacaacc taatcatttt ggtcgaaatt tctatacgga tgccggattg
     9661 gatgtcgaat cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct
     9721 ggtggaaacg tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa
     9781 gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta
     9841 gcgttgatga aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct
     9901 gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa
     9961 aaccaagaag aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc
    10021 aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt
    10081 attctttcac gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa
    10141 acggcaatcg ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta
    10201 aaagataaaa cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt
    10261 ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt
    10321 atcctcttgt tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt
    10381 atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt
    10441 gccacaactt tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc
    10501 tttcaaaagg tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga
    10561 ttaaaagaac gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca
    10621 gcagctactt tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat
    10681 ttgatcgatg aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta
    10741 gatcaagtta ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa
    10801 tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt
    10861 gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt
    10921 tcagcaaaac gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac
    10981 tatgatttag aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa
    11041 ttgaaggaat tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt
    11101 accgaaaatg agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt
    11161 gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt
    11221 ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg
    11281 caagacccaa atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa
    11341 accgaattag cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg
    11401 atcgatatga gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca
    11461 ggctatgtag gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat
    11521 acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta
    11581 caagtcctag atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat
    11641 acggttctga tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct
    11701 gatggaacga ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc
    11761 aaaccagaat tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac
    11821 aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa
    11881 gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa
    11941 ccagcatatg gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta
    12001 gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt
    12061 gatggacaac ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag
    12121 actataaaag aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa
    12181 cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt
    12241 ctgtcacaac ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta
    12301 acgctcatct tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt
    12361 atttgtcagc aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc
    12421 atcagaagca gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca
    12481 gaactcccaa aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca
    12541 aggactggtt tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag
    12601 agctgtcagg accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact
    12661 gatcaaatgc atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa
    12721 aacgattgct gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat
    12781 caatgcaatc cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt
    12841 ttgtgtgata aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc
    12901 tatttgcacc tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta
    12961 cttctgaaag caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta
    13021 ttgtatataa gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt
    13081 ttttcgggtg ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc
    13141 ctttcgcata agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta
    13201 gtaaagcaat cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc
    13261 gactgtctgc ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg
    13321 aaagctcaga agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac
    13381 ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa
    13441 aaataatcgc taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt
    13501 tcttcatctg tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc
    13561 atcaagcaag tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag
    13621 acacggactt gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg
    13681 acaggatctt caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga
    13741 aggaattctt ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc
    13801 tgactatctt caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag
    13861 tagatcatgg aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca
    13921 agatagaaca tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct
    13981 cttggttcat ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa
    14041 cgccaatact cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca
    14101 aatttgtcat aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca
    14161 cttactggga tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac
    14221 atgatttccc atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc
    14281 cattgcttat tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca
    14341 tggttagcat attctggata agttccaact ccagtcacac cacccatttt ataacgtgca
    14401 ccgtgaatat tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg
    14461 aaccatccga acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt
    14521 ttattctcaa acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc
    14581 aattcattga attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta
    14641 aaatcttttt gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat
    14701 tctacaaaaa aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt
    14761 tggtataatg gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat
    14821 gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa
    14881 atctacattg gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca
    14941 agatcagctg ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa
    15001 tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga
    15061 caccccaggg cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg
    15121 tgccgtttta gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta
    15181 tttagcgtta gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc
    15241 agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga
    15301 agctgtatta gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt
    15361 ggaatatgtt cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga
    15421 ctctatttat gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt
    15481 caaacccgga gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt
    15541 cggggtcttc tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta
    15601 tatcacagca agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc
    15661 agacaatcct gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg
    15721 tggtctttat ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact
    15781 gcaattgaat gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg
    15841 gttccgctgt ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg
    15901 ggaattcaat ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac
    15961 ggatggtacg actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca
    16021 ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc
    16081 agtaatggaa ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga
    16141 ttaccgagta aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga
    16201 caaattgaaa tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca
    16261 aaaaagcaag ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag
    16321 ctttatcgtt caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa
    16381 aaaactaatc ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat
    16441 cgttgcccgt tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg
    16501 agatgtttct cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa
    16561 acaaatcggc tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggatga
    16621 gcaagatagt gtgaaataac ttataatcaa tcgaaaaacc tattaaatca acctttttga
    16681 gtagatgcaa cactgaggat tatctgtata aaagatttta aactgtcatt tggttatcac
    16741 ggcgatacta aaattaacaa aattggaata tttttcttga agatttttag ctgtcagtat
    16801 atgatgaaag catctcatag gaggtgcttt tttcatggtt gataattcaa gtggaagggt
    16861 tcttaaatct ctaagaaaag agaaaaaact atctcaaaaa aagttagctg atttagcagg
    16921 tatttcacaa agtactttag ttaaatatga aaaagggtca agaaaaatcc ctaaagacgt
    16981 agataatact ctaagcaaga ttctgaatat agaaactcta ataaaagatg aggaagataa
    17041 gattgagatt ctcatcggtc aactaattgc atacagagat atgaataagt tacttaacaa
    17101 agagttagct gataatatag gaattagtga agtattactc agttatgtgt taaatagaaa
    17161 gagaaatccg tcaaaagaaa tgcaaaaaaa aatcgatatt tttctattaa gtaatgaaaa
    17221 agaaattttg aaggagatta atcgtgatag tgagatattt agtttatcaa aagacgataa
    17281 aattgttatg ggaaaaagaa tacgagaagt ccgaaagaac cgggaagaga cgcttgaaaa
    17341 atttggtaaa aactttacca tctatacagg caaaaatgtt ataagtagat gggaaaaagg
    17401 tattaatatt ccagatattg aaaaactaat gaatattgcc tatcttggta aggtcactgt
    17461 tccttacctt atgtacggag aagattataa gaatatactt ccaaaagatg aaagggttag
    17521 cgattttaaa aagataaatt cttttagtat gggattaagg atgaggaaaa ttaggaaaga
    17581 ttattatcta gaaagggagg agtttggaaa gcttttttct ccttcaattt caaaatggtc
    17641 catagatagg tatgagaatg gtagagatat acccaatact aatcgaatta tccaatatgc
    17701 ttatatagga aatctttctt tagaattttt gatttacggg atataaaaat agtttacgtc
    17761 gatacttatt aagttatcga cgttttttta ttatagaaaa aatttataaa aggaggtttc
    17821 ttgatatgat aagaaacaaa aagtgttggt tgaaagttgc tttactggca gctttttttc
    17881 taagtaactt tttgacaagc tcaatggcaa cacaagcgga agaagtaata caagaaataa
    17941 ctgttatgaa taacagctag ataaaatggg ctaggtatgt tatccaacaa tttagtaatc
    18001 actagaagcc tagcgaaagt tcgcttgata agttatagat gagtattgat ttgtagtgtt
    18061 tccaaatcaa aagggtacag agccacatga aatatgttgt cctctgcagc gtcaggatgg
    18121 gaatccatac catggagatt cggagatctg tgagggaata cttacgtggt taatatgtcg
    18181 gttgtgttgg acgtagtaat ggtcatagtg ggtagattga ctgaattctt atatgagaaa
    18241 atgaaaccaa caagaagata gggttattta tacagaattg gggatctttt ctggaaaagt
    18301 aatcggacaa tgcgagattc gtacggtctt tcttaaattt ctcttaaaaa aaacggcatc
    18361 gaatgggttg ggtgattgat aagcaatgaa tcatagactg atatagaata aaaaatgatt
    18421 ctatttatct ttttacatct atcgtttttt caagtaaccg caaattgtat cagcattcaa
    18481 caatttttgt atacaagttc tagtaaaagg atctgaagaa aaagttggtt gttgtgaaga
    18541 taaaaaaaga atcaggtttt actgagtttt tcttcaaaat ttgtgttatt aggttaggac
    18601 aaagataaga agaagtatga agatttaaaa aaggaatgag gaaaagatag taatttccga
    18661 ttattggagt ttttttattt ataaagaaga agcgaataag ttgtgattcc tagatttcgc
    18721 gcgtgattaa ttaatgagca aaaaatgcgg aaagttttag tgtttaatac acgaggacaa
    18781 agtttcttag gtattgaagg cacacgtgta acggttgatg ggtggtgtga ttgtttcgag
    18841 gttggaaaac atgttgaact tatggcattt actgagctaa aagataagaa caacgtggaa
    18901 tttttcgaga gtgatattgg ttgggacgat catgaagaag tttatggaaa atttattttg
    18961 cggaagggaa attcttatac gaatggggaa gcataagtag cgatttgtta gagatcattg
    19021 ataggattga aattgtcggg aatatttatg agaatctaga gttatttgag gtgatagaat
    19081 gagtgatggt tttgaacaag tatcaataat taaaaatcaa gttcgggaga ttcttagaaa
    19141 aaaaggctat ttggtagata gttattttga aggagattac gaaacatggg taggtgtcta
    19201 tgctcgtcca gagaataaac ctacctattt agatcctacg acgtcagaag atgcctattt
    19261 acaaaatagg tatcgagtag atggttttaa gcaagacttt gctgagtggt ttgaatggga
    19321 aattgaaaac ggagaagtaa aagaagaata agcgtacttg ttgtatcaaa ataagccagt
    19381 agaacaacca aaccaatagc tagtaatgac aataatactg aggaatcgga tcattatggt
    19441 gtgattgata tttctcttcc agaagatctt aagccagatc ctgcaacaat tggcaacagt
    19501 ggcatgattt ttgattcgtt agatgaagct atgtcatatg gttggaaaga aattaaggat
    19561 gaaggtagta attggtatgg catgagattt ggtgatggtg aagttgccaa taaagctggt
    19621 attccaattg ggaaatggac agttgatttc cacgaagcag gttctttttt gttctgtaat
    19681 tatagtggga gggtaaaatc attgatgaaa tgtgttcaga taagagcgcc agataaaaat
    19741 agaagagaca gttcacgtcg ttatcacttt tggtaacggc gttttgtttt attatataaa
    19801 attctcagga aaaggaggaa ttcttgtgta tgcaaaaacg aaaatgaatt ggttgaaagc
    19861 actcgcattg gtggcctttt ctcaattctg tttcttggtg ggaactgctc aagcacatgc
    19921 agaagaaacg acggctacac aacctacggt ggaagccatt gcccaagtcg cttctgaagt
    19981 gacagaagtc caaccagcag aagccggggt cccagctggt gaagccactg ttgaacaggc
    20041 ccctgtgaca gaagcgccaa cggtcaattc caaaacgact ctcgatcaga cgattgggga
    20101 aacacaacac ccagggaata gtcctgttac ttcacaacca gttgaagaaa ctgcctcaat
    20161 tacaacccct gcttcagaaa cgtccactcc agaaggagaa aaagctgccg aagcaacgcc
    20221 ttctgtgaag ccagagtctt ctgagaaaca agcggaagaa aagcaacgaa tagaagaaac
    20281 agtggaacaa ccagcggcta ctgctgacaa agaaggaaaa aatgaggaaa acgttgaaaa
    20341 aacaatctct tcaaccgcag ctagtcaaaa tcaaccaacg aaaccaaaca aagaatctac
    20401 ggctagtatt gaagatcaaa agaaaaaaga gcaggacatt ccaaaaacag gtacacctac
    20461 actgttagta acagtaccaa ccaatcgttc ggttcgtgtt cgccgtgcaa ccaatacagc
    20521 tgttttaggt ggtcggaaaa ttgattgggg cgaaaatatt ggtgttactt ctagtccgaa
    20581 caatccaaac ttcactttaa attatatggt tcgttttgag attaatggac aagtggcgtt
    20641 ttgtattgag cctggtgtct cagccgcaaa aggtcaagtt tatacagcta ctgctttgga
    20701 aaactatttg aaaaattcaa atgtacggaa aaaaacaagc ttgatctctt atttaggata
    20761 tgtcacaaat aaagacaaat ccgatgaaca atacattgcg gcacaattta tgatttggga
    20821 agctttgggc acaaaaatcc caatcaaaaa tatccgtgtt gattatgatc gtcgaaaagc
    20881 agaaattcaa cgtctgctta ataattttga tgtaagagcg acgtttcata atcaaacaca
    20941 tacgataaaa attggcgaaa cgttaaaagt aaaaagtgga aatgactttt tacaacgtgt
    21001 aaaaggcatt caaaagccaa aaggtatcaa tgtatctatt catgggaatg aagcatggat
    21061 cacagtcaca aaagaagcac cagaaaaggc aaaaattcat ttcaatcaga ttgaagtatt
    21121 gggtacgcca ttagcgtatc gtcgtccagg tgcccaaaca attggtatat tgaatccttt
    21181 tgaacctggt gactctgttg tcaatttgac tattttgaaa caaggaaaca ttcgtgtctt
    21241 taaagaagat gcagaaacta gtgtgaaagc acaaggtgcc tcttctttga acaacgccgt
    21301 ttatggtttg tatcaagccg atgggaagaa aatcaaagaa gttactttga agaacgtcaa
    21361 tgggaaagtt caagcagaaa ttaaagacct tgatcctggc aaatatgttt taaaagaaat
    21421 caaagcgcca aaaggttatg tgctttctga tgaagcaatt gaaattgttg ttgaacctgg
    21481 aaaaactgtc aatgcgaccg caaaagacca agtgattaag ggcaccattg atttagtgaa
    21541 agttgctaat aaagacttaa tcgattcaat gaatccagat aataaaccaa aattagctgg
    21601 tattgaaatc tctttgacgt caaaaacaac tggaaaagtg gtaaaaacag ttgtgacaga
    21661 taaagatggc tatgctactt ttggtaaaaa tgccgttgtt tttgatacgt atattctctc
    21721 tgaaaccaaa ggaaaagaag ggtataaact atttgaacca tttgaagtca caatttctga
    21781 aaatgggcaa actttccatt atgctttaga agataaagta atcgaacaac gtcttaaagt
    21841 ggtgaaagtg gatcaagaaa caggaaaagt gattccactt gccaatacag aattcaagat
    21901 ttttgatacc ttagccaatc gttatgtcac aatggagatt ccaaatgata cagaaacaac
    21961 ggatatcttt aaaaccaatg aaaaaggctt ttttctaact aacggtacat ttacttatgg
    22021 aaaagatcgc tatcgtttgg aagaaattaa agcgcccgta aattacgttt tgaatcaaaa
    22081 ccattggtat tctcgattac caatgataca gaaccaatca aagtgatcca ttttgcgaac
    22141 cgtcaagcac ggaaaaacgt gaaattgttc aagtatgaag aatggaacaa gaagaaaaca
    22201 ccgcttgctg gtgtcacctt tactctgtat aaccaagcag ggaaagctct tggtgaatat
    22261 atcaccgatc aaaatggtga gattttagtg aaaggtctag ttgccaataa ctattacttt
    22321 atcgaaaata caccattaga aagtttccaa ccaaatacag acaaacaagc attcactatt
    22381 acattcaaac aaaatgcgcc agatgatggc gaaacgttat tggtttctgt acgtaactac
    22441 ttgattccgc caaaactaga aacacaagcg tcaaataaag ccgatggtga aaaagtggtt
    22501 gatccattag aaacgatcgt cattaaagac agtgtttctt ataccaacct gtttgtcgga
    22561 aaagaataca ccatttcagg agtattgatg aataaggcaa cgaatacacc gatcttacaa
    22621 gatggtaaag aaatcagaaa aactttgacc tttattgcag acaaagtcaa tggcacaaaa
    22681 gaccttgaat ttgtggtcaa tgccagcgtc ttaaaagggc aaacgatcgt tgtctttgaa
    22741 aaacttttcc gtgatggcat tgaagttgcg gcgcatgcga atatcaatga tgaaaatcaa
    22801 agtgttcgag taaacaatcc agatgttcat acaaccgctg tatttgaaga tggattgacg
    22861 gtcgcagacc caacggggct gtttacctta aatgatatgg ttaagatcaa agaaatcatt
    22921 ccaggaaaag cttacttggt caaagggatc ttaatggata aagcaactgg tgaagcctta
    22981 cttgttcatg gtcaaccagt agaaagtgct ggtggcttta ttgccaaagc agaaaatgaa
    23041 acggtgatcg taccatttac ctttgatttg acaggtttaa atggtcgtga aatcgtcgta
    23101 tttgaaagtc tttttagaaa tgatgaagaa ctggcaaacc acaaagacat caacgacaaa
    23161 gggcaaacgg tacgagtaac gaacccaagt gtacgtacaa cggctacgca taaaccaact
    23221 ggcttgaaag tggttaatcc attaatgaaa atcaccatta cagacgctgt gaaataccac
    23281 gacttaatcg ttgggaaaac atatacagta aacggtattc taatggacaa atcaacggaa
    23341 aaaccagtgt tggtagacga caaagaaatt actagtacgt taacgtttgt ggcagaacgt
    23401 atcgacggta cagtcaatct agactttgtg ttggacgcac gtgcgttacg aggaaaagaa
    23461 gtcgtggtct ttgaagatct cttcagagac ggtgtattat tagcttctca tgcggatatt
    23521 accgacaaag atcaaaccat caaaatcgtc aatccaacca ttacaaccaa agctaccaat
    23581 aaaaatggtg gaaaagagat cttggcttta ccaaatcaaa ccgtgttgga atgggttaaa
    23641 gtagacggtc ttgcgattgg tcagttgtac aaactgcttg ttcaaggcta cttaacacca
    23701 gacggcacgc catttgaagg tacacatgca gaaaaagtct ttacggctga taaagaaatg
    23761 atggaattct tatttgaatt cttagtggac ggtcgcaacc ttgcaggaaa aggcttaagt
    23821 tttgtggaat ggcttcaagc caaaacagaa gatgacaaag aagcgtttga ggaagtcgta
    23881 aaacataacg tggatttaac aaacaaagat caaacggttc aatttgtgga ggcaccaaaa
    23941 ccaccgcaac cagtgaaacc agaacaaccg gttaaagcag ctactgttgt accaatgttg
    24001 ccaaaaacag gcgaacaaac aagtagtata cttatggttc ttggattagt agtattagtc
    24061 attgttttag gtataacact ttataaagta cgtaaagcca aagattaggc atgaggaaca
    24121 agcggttagg atttttaacc gcttgtttga ttgtaatgta gaaatattta gaatttattt
    24181 tggtttttaa gaggaggaaa aactttatgg cagaaaaagc attgcgtatg aagaaagagt
    24241 ttttaggtca agtattttta ttgggttcac gggaacctaa tccaccaaag gaaattaatg
    24301 gtgtgtctgg ccgatatgtt caattacaag cagaatgttc gcgagaagaa cagcctgctt
    24361 tttttgttta tctcacagct gaagaggata aaagattacg attaccagaa aaagaggtgc
    24421 gtatttcatt tgaagaagct tattttgttt ttcgtaaaga acgagtgaat agtcgtgaaa
    24481 caaatgatat gggacaaaca gagcgtcgaa caactggcta tcaagatgtt ggtgtctttt
    24541 taactggcgt aaaaattgag gaggttttgt aagatgggat taaaatttcc aaataattat
    24601 gatattacag aagcattaga tgaaacttgt gtaggagccg tacgctttgg cgcattagaa
    24661 gagtctgatc gtgtatttga taatgaaaca ggcgattata cgggagaaat tcgatcacag
    24721 cgagcacaat tagtttttgg aaatccagat gaaccaaaga ttgcacgacg agttaaattt
    24781 ccagcagatg tagatttgac tggttttaaa cgacgtgatc ctgtgaaatt aattgatcca
    24841 gaatatcatg tggtatatat tccagcacaa gatggcaatc gtgaatattc agatttagtg
    24901 atttatgcga aaggattaga gaaagtagaa gctccaacaa ataatcctaa tctgaaacaa
    24961 tcacaagaaa aaggtttaga tcaaaaacaa ccaagtgttg attctaagga aaaagagaaa
    25021 aaataaggat tttttgaaag agtaggaaga agtcttccta ttctttttat ctaaggtaga
    25081 aggaagggga gattttgaat tcatataaaa ccaatcaaac acttcgagag attgtggctt
    25141 gttgtcaaaa tacccaacaa ttgattgagt attttggaga caatccgacg atttttgcac
    25201 aaacaaatgc atatatttgt gcggttattg ggcagtgtat gtttttacgt gatcttgttc
    25261 gtttttcatt accatatcag tggaaacaaa atcatcagga agaggtttgg attcgtgtag
    25321 tacgaatatg taatatgtgt gcacacccat aaaaaaatcg attagatcgt tttcaattat
    25381 ggttttttat taaatgtgat attccagtat tagttgcttt ttgtcgaaag tttttagcac
    25441 aggagaagta taaacagaaa ggaggaatgt gacaattgga acctcatttt tttaagtatc
    25501 gtggtcggcg aattcgttat ttcaatcgtt atttgtggtt ctgttattat ggtttctttt
    25561 tactcccatt tttgattctg ttaggttggt ttagttggaa agtattttat ccgcgtttaa
    25621 gtacgtattc gaattggcaa atgtatctta ttcctggaat aagtattgtg ttgtatttat
    25681 tgctggttag tggattaatt ttaggtttga tgcgttggtc gcgattaaaa gaaggatatt
    25741 ttgcctctgt ttattggcgg caattacttg ctcgaatgtt aattgataat cggtttattt
    25801 atacaaagaa gcaagttggt gagaaacaaa tgaaggaaaa aatgagatta ccaaaagttt
    25861 atttttatca aaagaaacaa gaattagtga tttcttttcc tttagatggt gggcgtttcc
    25921 acgaacgatt tttaaaaatg ggtaatttgt tatctgaagt atttttgcgt gatttaattc
    25981 gtgaagaaag agaaaaagcc cgaatcaatt atgtatttct ttccgattct ggtcggattc
    26041 ctattgatca atgtatagca gaaaatggac gtattcattt aatgaaaacc ttggattggg
    26101 tatatgatca atcgccaaat ctgttaattt ctggtgcgat tggtggcgga aaaacctatt
    26161 tattgtattc attgatcaaa gcttgtttag atgttgggac agtagatatt tgcgatggaa
    26221 aagcggcaga tttagcggct ttaggtgacg ttgatatatt caaaggacat gtgttttaca
    26281 aaacgaatga agaaatgatt gcttgcttac gaaatgcgtt aaaagaaatg aatcaacgat
    26341 atgcctatat gaaaacgatg aaagaaccta agtatgaacc agggaaaaat tatgcttatt
    26401 atgggatatc gccgcatttt atcttttttg atgaatgggc agctttttat ggttcgttgg
    26461 atatggaaac aagaaataaa gtcgataaat tgattcagcc attagtatta aaaggtcgtc
    26521 aagcaggcat gtacgttatt ttagcgatgc aacgaccaga tgcggaatat tttccaagtg
    26581 gtgttcgaga taatttaacg ttccgtattt ctgttggtcg attatctccg attggctatt
    26641 taatgacttt tggtgaagat tttaaaaata agccatttta caacaaagat caacctggtc
    26701 gtgggtatgc ttttccagga tttgatgtac ctgtagagtt ctttgcgcca tttgtttaca
    26761 aagactatca ctttatcaaa gaatttgaga aatttactga aatgattgtc tatgatttta
    26821 cagtaaaaga ggatagtcaa gatcaaacga tgggagatga taatcagttg ttacaagaac
    26881 aactagagcg ccaaatgtat ttagcaaaag atccaaaagg aacaagtttt tctgatgcgc
    26941 atattttgta atcaactaag gaggtgaagt taaatggcag agagttgtta ttatcgagta
    27001 gctacagcaa tacgaatgat taatccttct ctttcttcca gaacgtttta tgattggttg
    27061 aatcgtattg aacaagtaac tgattaccgc tttttgcgca aagaacgtgt ttttacagga
    27121 aaagttatta atcaagtatt gctaacgaaa aaagatatag aacgattgac tcgattgtat
    27181 cattatcgag taggtttaga agaggattta actttgtcaa tttatcgcgt tttttctcct
    27241 gaaaaatata gtgaaataac aaaattagat catttaattt tgtagttatt atctatcggg
    27301 agggctctag acgtagtctg gtagccgcac tctgcctggc ggtgcgcatt aaggctgcga
    27361 agcatgggcc tataatgcgc actcgctagc agcgtgtgca gctcacaaag aggtcgtgcg
    27421 cttgcgcacg gcctctttgt gtaaagcccc ccgatatata atatgggggg cagaaataac
    27481 ataacacaaa taaaaaagta tatttaaagc agttatatca aggctttagc taaagttaga
    27541 agaagtaccc ctttttagaa aaatcagggg gtaccaccta tgataccggc atttctagca
    27601 gattgaaaaa atcgaaattt gttattttat aaaggaggta gaatatgacg ccaattgaat
    27661 tgcgaaatta tcgaaaaaaa ttgagtgcta ttcacgcttc aggtggaggg aagaagttac
    27721 tctctcaaaa acgttttgca cagttattgg gatgctcagt agattatata aagaagatgg
    27781 aacagggaaa aaggaaaatc ccagaggatt ttgaacaacg aatcaaggaa cgctttcgat
    27841 tgtatgtgga aagcggaata gatcttcgag caatgattga ttatttgcgt ctttcctttt
    27901 ttgggacaga ctacaaaaca gtgattcaac gtgtattacg aatggatctg acagaatttg
    27961 aaacacgtcc aacgggcttg tatctttttg actgtctttc tgttcgtggg aatatctggg
    28021 tgttctggca ttcaaaacga accgtaacag aaaatgtact tgtacaattt tcaggacaag
    28081 gttgccggga atatgaggaa gtcatgcgtg aacaacaaac agattggcga acatggctat
    28141 attctatttg gaaaaatcaa ggaaagttgg aagctggtta tacacgtgtg caatgtagtc
    28201 gattcgatat tgcgttagat gagctttggg gattggaaac ggaagaacat ttcgatctct
    28261 ttgaattact agaaaaacaa aaagcgggtc tattagaaat ggattttaag agattccaga
    28321 atattggcgg ttgtttttta gatgatggct attttcgaag tgaaggatta tccttgtatt
    28381 ttggatcgcg aaagagtcca ttgtttttca atttctatga aaaacgtttt gaaattgcga
    28441 accgagaaaa aatttcagaa ttagaagcat taacgaaata tgggatttat aatcgatatg
    28501 aattacggct agccaatgag aaagcaacac aagcagtaga agcatttatt ttaggtgatt
    28561 caccgaaacg tttaggagag attggcgttg gtttaattaa tgcaaagctt attgtttggg
    28621 atcgactgga aagtgatgca cggattgttg atgaacgatg ggcaaaattg tttcgttcga
    28681 tggaagaatt ggattttaca gtaaagccag aaaaattctc attagatcgt tcagaacgct
    28741 ggtttttgaa acaagtagct ccgacattaa aattgttaca agaagcagat cgtattttag
    28801 gaagagactt tgtacaagaa ggcattgata atgctgaact aaatgacgag caacaaagct
    28861 atctagaatg gatgggaacg tcaggaggga aaattgcatg aactcatata tgacaacaga
    28921 atggttgcgc ctgttggatt taaaagctgc gatcgaagca ttaaatgaaa agatggttga
    28981 tttgtcttat tttcgttttc gagttcctta tattgaacaa gcggtaaaag ctggacgtta
    29041 tcaggaaaaa gagaactggc aagaaattgc acgcttatta gaagttcgta agggatacga
    29101 acaagaactc gaagagttgg aatttagccg aaggaaaggt aggctagaat ccattcgctt
    29161 ttatcgattt tcattgccaa tacctgcgat tttagcggtt aaaaaaggct gcgataaaat
    29221 gaagatatac gaaaactgtg tggcagcact tagcagcgaa aaacctctta tggaagaaat
    29281 ttcattggcg actgtcactt gggaaatgag tcgccagcca acagaagaac agacatatct
    29341 ttctttagaa gagattgaga tagaattaga ggaaattggt cgctatgcca cttgttctac
    29401 ctattgtgga tcggtaatta gtattgctgg tgtgattgtc taaatgaaaa aaattatgat
    29461 ttatgttggt gcgtattggt cacgtgatcc aactgtatta gaaaatccag aggcagtaca
    29521 ctattgtttg cgccaattat tctatctgta taaagaacga ttagaatcgt tgattcggca
    29581 gttgccatat actgacagac gtttagatga actgcttcta aggtatccag caatgtataa
    29641 acgacgaaaa aatagattgt taccagaaga gtatcccatt gaaaaacgtg aacttgaagg
    29701 gcaatttgtg gcttatttct atgatgatgt tcgtatgcgc ttagtagaac aacgaatgga
    29761 aatagaaaat gatcgctact attttatcaa ttattgcaag aagcgtaagt atcaagtaac
    29821 tgatgatttt tatgattgta ttttacaaga tggggaagtg attctatcca aaattgctcc
    29881 aagttttcgt gaacttgtgc cgattgattt tttgaaaaaa tgtcatttgc ggattcttcc
    29941 gtaaaaaaga gctataaaag gaaagaaaag tagaagccaa atcggtttct gcttttttat
    30001 tttttaagga agcagaagat ggtatgtcac tggaattttt aggagagcca attgaaaagg
    30061 aagagttcgt tttgcattat atggatttgt ttattcagtt aataaaaaag ctttgtgcga
    30121 ttgatgaatt ttatccgcgc gaacaagact atctagccgc tgaacgaata aaaatcagtt
    30181 tattatggga aaaattcaca gatgaaaata accgaccgcc agattatcgc tatttatcag
    30241 attgtttaac gaataatgtt ctttccaaag agtatctatt tatcaaacgt gagaaaaatc
    30301 aaatgtcggt tgaacatttt tgtgattgtt atattgcatt attgcgtagt caacagttat
    30361 taacggtaga acgatatggt gcagagtttg gttatgtcat gcaagctgag cgtcatagag
    30421 ctacaagatg ctatgaaaaa gaagatcaag tgattgatgg ctatgaagaa ttatgtattc
    30481 gaaacaatcg ctatttgcaa caacgtttat atgaacaatt gatgatggat ttttcaaatc
    30541 tctatagagg gtatgaatgt tcgatgattc aagaaaggta gaagaaaagg gggaattttt
    30601 atgtggcgat tattattttt ggtggtatgc ggtatggcat tgttctatgt gtatcgtgtc
    30661 tatctctatc cacgaatgga acgatggaca agttggtata gagatccttt aaaacaacga
    30721 aaaggattga agcggtatcg ctatgaatta cttgttgaat tttcagatgg gcgacaagca
    30781 cagtattatt ttttttgcaa actatccaaa atacaacacc agtgaactat tgattcaatt
    30841 attcaaaaat aatccatatg ttgggttaga agaaaacgga cgagagttct tttatcgttc
    30901 agataatatt agtgcggcgt ctatgaacaa aacacgaatt gcataaaaaa ttggaggaat
    30961 aatcatgata aaaacaacat tagcagtcgt aacaacagta gggattacac tattttcgtt
    31021 tcagttagga acgttattgg taaatgcaga tgaaactgta gaaacgccta agtcagaaat
    31081 tgtagacaca ttgccagggg aagagttacc agatgttgag acagaactaa gtggaggagc
    31141 aagtgaaact ccagaacaat ctcaaccaga acaacctgaa aaagaactaa tagagtcgga
    31201 accagagttt cctgagtcaa ctttaccgac tgatccaatc gaagaagaag gtgtgcatgg
    31261 ctcaacaaca acggattcaa gtacagtcac aactccagaa caatcaggaa caactagttc
    31321 ttctgaaaag actaccgatt cgtcaagtac agaacagcca acgatgtcgt ctaccagtga
    31381 agaatcaaaa gaagaaccta gtcaacctgc tgaaaaggca agcgaaaaat cagaagcaat
    31441 tccaaaagaa aataaacctg ttgttccagc aaaagaagtc acagtatcgg taaccccaag
    31501 cggtgaaatt acgacgaata cttctcaagg aaccagtgta ccgattgtca ctagtaatgt
    31561 agaggaattg acccatattc caacaccaac caccccatta aaagtagaaa gtggtcaaac
    31621 aattgttggt gtaaaagatg gaattccatt agtccaagat agtgaaggga atttggtaaa
    31681 ggatttgtcg attcctgtaa agaaacttcc aagtggcaat attgaagtca aaacagcaga
    31741 tggaaaaaca aaagtcttac caaagactgg tgaagaaata catgttgtac tttctgttct
    31801 aggaggagtg ttgacaagta ttgctggatt tgttggatat aaaagaaaga gaaaaggaat
    31861 agaaaatgag ggtaattgaa tggactattt ttcagaaaaa caagcttatc gttgtcaatc
    31921 gtacgtacta gtaaaaacat actagaagga gtagcttatg aaaaaacgac aactacggcg
    31981 gaaagaaaaa gtttatacag agaaagtacc gaagcaaaag aaaatccatt caaaaaaagc
    32041 agtgaaatgg atttggtgtt tggttttatt tctttgtttt tcagggggct tggcattttt
    32101 tcaatccatg aaaacgaagg ttcaagtgtc gtttttgaaa gatgaaatcc aacaattaca
    32161 aaagaaagaa gtacaaagtc ggtcagaatt aacgtacaca cctgaaattg aatcctttat
    32221 gaatcgtttt gttgctttgt atatgaatac attcgataat catgaacaag aacaacaacg
    32281 tcaagaaaca ttaaaaaaag agttttatgc gcaaaacttt attcaagaac caggaagtgt
    32341 ttcaggtgtt cgaaaattaa ctgctgcttc gtttattgga ttaaaagaaa ttgaaaccat
    32401 tccaacagct agttataaag taacttatga tattcgctcg atgaatccag agagtaagaa
    32461 agaaacaagt gtgacaatca cacaagtact caatcttccc ttttctattt caaacaaggg
    32521 ctgtcgtatt attgcttatc cttattttac ggcactacca aagaatacag aaaaacaacg
    32581 aatgattact tatgatgaca cattatacga aacggttgat gcgtcggaag cgaaaggtgt
    32641 tgaaggattt gtggaagatt tcttagataa gtatgcgaaa aacaatgcaa aagatatgtc
    32701 atacatgatg aaagaacctg aaggactcaa tcagacagct aaaattgatc aagtagagat
    32761 gaaagtattg cgttataaag aaacgaaaaa accttttatt gtaaaagcaa cggtcgtctt
    32821 tcgagatggt gatacgtcac ttttacaaaa agaacaaatg acgcttgtta ttacgaaaaa
    32881 aacaaatcag tattatgttg aaaagctaac acataattgg ttggatacga aaggagaaga
    32941 acggaaatga cactgaaggg aattgtagat tttatattaa cgcaaggtgg ctacttttta
    33001 ataatggtgg ttgtttatct ggggttacga aattttatga aatcaaaagt aggaaatgca
    33061 ttaatatcta ttgcagcagg tgcagcagtc tatttctttt taaataatcc aacacgtgta
    33121 tttgatgcgt ttggtaaaat cattgagaaa ttatttatga attaggagaa tagaaacatg
    33181 gattgggaac cttatgatta ttcaattgtt tatgcggaac cttatcgcgt acaacagatt
    33241 acaagcaatt ggcgtcttcc atttccagtt gcattagtca atgcagcaac ctttttgttg
    33301 gtcgcaatct ttgaatattt atttttacgt tcgtttatta gttgggttat ttcttttttt
    33361 ccaccagcag gagttgtttt ttatataggg ctaccctatt tatgtgtgtt agcactggca
    33421 aaactaaaac ctgatggaaa aaaagtgcat ttttatttat gggattggtt ttgttatgta
    33481 gtgcgtatga tgaatggtga ttgtgtgtat tataactggg acaaggtatc aaacaatgca
    33541 ttggaagaat tactttttgt gaataagtat gtttatttag aggattagaa aaagggacaa
    33601 tcaactggtt gttccttttt ttattgggag gtgaaactta ttggtgcttg taaaagaacc
    33661 agtaaaagca tttaagaaaa attgtttgtt aaatggcgag gattgtatat gggcctacta
    33721 tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa aagacacagg aaataaaaga
    33781 aatgatggct cgtttactgt tgcttttagc agattatgaa gatgtggagt tggtgatgta
    33841 tccacgaaca atgaatttac gaacaaagtt tgacacctta ggtaagggat tttcacctga
    33901 tagtcaagcc attggtgcgt attatgcaaa agaaacagtg acattgttag aacgagaagg
    33961 gcaagtctat cgtccttcat ttattttagg aatcaaagtc aaagaaaaag caacggtaga
    34021 gagctttcat gatgtattaa atctaatctg gttagatgta gcaggaagtt tagcacgctt
    34081 atttggatta gaagtagatg atttggaaac tttgttttct gaaagtgatc cttatgtaaa
    34141 agaagtgaca caattacttt atagcttttc tattcgtccc ttagtaaaaa aagagctgtt
    34201 gtatctcaat cgtctgcaat atattcgagg aatggctcat aaagtagaag aacaaaataa
    34261 ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt ataccgaagg aacataaagg
    34321 ccttcttcaa ttagaagatg aattggggac tagttattta gcgttgttac ctattggaga
    34381 aacacctgaa aatatgagaa gttgtgatct atttcaattt gcacaggatc tttctttccc
    34441 agcagaatta cgaatgaaaa ttcactatcc acgcttgaaa ggacagggag gtattggttt
    34501 aaaactgaat tggctatcgg aacggttcaa aacagaacat aaggaattgg ttcaaaatga
    34561 tgaagaacct agtgaacgct tattaactgt tcggggatta gtaaaacatt taaagcaacg
    34621 agtagataat gggcaacctg tcgtcgattg gatcgcatgt tttattgtat acggtcaaac
    34681 ggttgaagaa gttcgtcaaa atagtgcaac gattattcaa cagcttcata cgattatcca
    34741 tatccaccgt gcaaaaaatg atcaacgaga attgttctat aaatgtttat taggacaacc
    34801 attgggacgg cagttacatt ggaagcaccg aacaactgta gaagcagttg gtgaatggtt
    34861 gttttcagca gtatcagagt taggtatgga atctggctgg tatattggtc gtcaagatac
    34921 attaggggac ggtggttcta gaggagagaa aacaccgtta gaagaattga tctacgcgtc
    34981 aaatcgcttt gtctttttaa atcttttagc cattgcggaa ggaatcaaag gtgcgttgtt
    35041 tgatgcgcct catgtagcag taactggaaa aactggtaaa gggaaaacat ttttagttgg
    35101 cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt ttatttgtag atcccaaagg
    35161 agaaaagcgc aaatggttca cgaaactcat tcaagacccg tattatcaga aacattaccc
    35221 tttgtttgtg gaacatttgc aaaaaatgag ttttgtcacg ttagatgcta caaatccccg
    35281 taattatggc gtattagatc caattgtttg tttatcccga gtagatgcga aacaagtagc
    35341 ccaggatatg attgatgagt taagtccgtt aggtaccaat caccttttaa aaggggcggt
    35401 attaaagggg atccaagaag ttttagttca aaaagaagcc gggcaacaag ttggcttgat
    35461 gcatgtggtc gagtatttag aacaaatgga acaaaaagag gttcgagaat atggtgagtt
    35521 tttacgcttg acggtagaag attcgatttt acgtttagga ttttcttatg gagaaaaccc
    35581 aggattagac tttaatgcca aaacaacgat tttagagatc caagatttga aattaccaaa
    35641 tgacaatgtc cgaccagaac tatatacaga tgcggatcga aagtctttgt gtttgatgat
    35701 tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat agtagtaaga aaacagcgat
    35761 ttattttaca gaagcatggg tatttaataa ttcgaacgca ggacgtagta ttattgcagc
    35821 aatggcgcgt gtggggcgtt cacagatgaa tcaattagtg ttagatacac aatttattgg
    35881 cgacttaggt tcagataaag aaaaagggaa ttttggtgtt gtcttcgctt ttgatgaaga
    35941 cagtgagcga gaaaagattt tgactcattt acgtttggaa gtaaatgagg cgaacttatc
    36001 tgaaatgagc catatgatta aaggacagtg ttggatgtta gatccctatg gacgtgtagg
    36061 aaaactcaat gtacacagtt tgtttgaaga aatgaccgct gcattacaaa caacggaaaa
    36121 aacaagtcac tctcaagtag aagaacagga tacgttaatc atgcaataga aatgaggtga
    36181 aacaatggct tatataaagc ataaggaaaa gaaatggtgc gttttgtttt ttttaacgat
    36241 tggattcgga ctgctttttg ttggtggatt ggtgtatgcg gcagaaaagc aggtggatca
    36301 atcgatctat gagattggga aatttcatat tgataccttt tcttcttatt tatctgatag
    36361 tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc acagattttc taaaaatgat
    36421 gacgaatatg ttctttagtt taagtaaatt gatttggcaa ggccttgatt ttgtcattga
    36481 gaagctctat gaaggagctg caatgaacga gttaattcac cagtttttca ctttttcgga
    36541 tactatttat tcaaaaatat acgatcctat tggtgcgggg attgtcgtct tgtatgtgtt
    36601 atatctattt actcttcgta tatttagagg gaaacattac gcaaaaatgg tgtttattcg
    36661 atttctcttc gttgtcattt tttcaatggt ttggtttggc tatggtagta acgtacctag
    36721 taaaggagaa gcttttacca aagggttaaa tgatttgtcc gtggaggtag aaggctttat
    36781 tttttctgct acaaatagtc tagatgaatt ggcgacaaaa gatgtcaatg gtgttttagt
    36841 gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa ctttattata aagcggctgt
    36901 tgttgatccg tatctattgt taaattatgg gacaacaaat ttagaagaat tgtatcaagc
    36961 agagattgaa ccacaagaat ttctagccaa ggaagcgtca agtgaagaat tatctgatat
    37021 tgagacaaaa gtgaccactg ctgcaggaga agatagaggg aaaaagaagg cttatcgtgt
    37081 ctatattcaa ccaactaaag ctgtttataa agcagtcgtt ggttctatgg tgccattgct
    37141 taatctaagt attggattac cacttgtagt gattggcatg attcgtttta tctttcaaat
    37201 gttggtgttg tgtttattac ttgtgattcc tttttcatta ctcgcttctt ttgttccagt
    37261 atttgattat atgctcatga ataccttaaa gtcctttttg ggcttgatgt ttcaaaaatc
    37321 tttttacagt gtgattattt tggtggcatt tttgatctat aacatagtag acgatttaat
    37381 tccaatgaat actgtttttg gttttttagg aaatatgttt gtcaaagcac tcctatcaat
    37441 tttactcatt gtaaaacgga aagatttatt aaagaaacta gggttaggag ctgcagatca
    37501 aactataaac aacacgaaaa acatagcaag tcattctttg tctcaaatga aacaactgcc
    37561 tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa ggtgcaggat tagcagggaa
    37621 tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata catggatttc aaaagtttca
    37681 acaaaaacgt acaccacaag aatcggtaaa agggaggcaa gtgattaatc cctttgaact
    37741 aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt catgttcgtt atcatggggt
    37801 agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg aattctggtc tttcaaatgg
    37861 tttgattcca aattcgtcca tgaattcagt aagaccaaaa caacaggtac aatcgttggc
    37921 acaatcgagt aagcaacaag ggaataatca aagaaaacca ttgttaaata aaggtatcgc
    37981 ttttgttggt caatcgattc cgttgcctgc tggaaaagta cccgtgccac aattaacagc
    38041 tgctggttct ctttcccaac agattcgcca agtgaaaaat caggcgaaaa atgggagtta
    38101 tccagcaatg cagcagttat tgaatcaata ctctgttcct aagtcgtcgg tagccactct
    38161 atcaggaaat cgttctgtgc aacaactggg acaacgttcg tctgaaagta aaccgtcgat
    38221 ttccgcacca aaatcgttac aacctgcatt ttcttcttta gataagtcaa cagatcaatc
    38281 gaaaaaaata gaaaaggatc ctaaaacact gccgattttg gatgcaaaac taggaaccga
    38341 ttttacttta ccaaaaggac gaacagtcca aaaaccattg tcgaagaaag gatgaggaag
    38401 aaatgaagaa agggtgtcta ggttgtcttc ttttctttat tcttcccttt tttctattgc
    38461 cattcttttt ttcaggagaa ctttccgagg atggaggaga ggctacaggt ctagctgtcg
    38521 cttttaatgg ggaatatacc gaaggattgc cgattttttc agagattaga ggacgtggtt
    38581 cgatcacaga tgaacatgcg caagcagcag ttggtgcggc ggtaaagtat cagttgttgc
    38641 cgagtgtgat tctttctcaa ttgggttatg aatcggctta tggacagtca caatctggaa
    38701 gaaacgatac gaactttttt gggattacct ggtttccagg ctgtccctat ccacaaggtt
    38761 ctgcacgggg gattggcggt tctgaaggcg gcttctatat gaagtttccg aatctaaaag
    38821 cttgttatga ctattacggc tttatggtgg cgagtcaaag caactttaat caatgtgtgg
    38881 gcaataaaga tcctgggagt tgtttattaa ttctaggtcg tggaggctat gcggcggcag
    38941 gtattaccga aggcagtccg tattaccaag gagcgatgag tatcataaag tcttataact
    39001 taacagaata tgatactttt gcgatcaaac attggaatag cctaccaaca aaaaaatcgt
    39061 cacttgttgc agggaaaagt aaaggtaatg cgaaagtttt agatcggatc attggacgat
    39121 cggtaaataa tggagagtgt tatggtggca cggcgtggta tgtatcccaa ctaggtggcc
    39181 cacagttaat gggttctgga cacatgtttg cgatgttaat aggggaagat tatgattgga
    39241 cagcctatgg ttggcaagtg atcatgaacc caaaaccaag tgatttacaa gcaggagatg
    39301 tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat ctatggtcac accggaatta
    39361 taacagatgt ttcatatggc ggacaacatt tctctagtgt ggaacagaat gccgagcaag
    39421 gacggattat ggctcggtat caacgcacgt acaatcaaac aaaaattcgt agtctggttc
    39481 gtaagatgaa ataagaaagg cagtgactag aaacgtgaaa aaagggataa ttttagtatt
    39541 tagttggtta tgtttgatag gtcttactgg ttgtcattgg ttttcttctg aaaaacaaaa
    39601 aacacaattg agaacgacag aagaaacaat cgaagcagta aaagaaacaa gtggaactct
    39661 tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa gacgagacag cgcaacaaat
    39721 gcaaatagat gaaatgacaa aaaatttctt aaaagatttt gctagaaaat ggagtaattt
    39781 cacagatgtt tataaacgca atcaagctgt gagggattat atgacagatc gttgtattaa
    39841 agacaatagt attgatgtag acccgcatgt acaaatgaaa acaactggga aactttacca
    39901 ggtgacacaa gatatagaga atccggatca atatttgtta tttggagaag aaacaatgaa
    39961 taaggttact caatttgtat tacttcaatt gaaagtagat caagaagctc aaaagattga
    40021 tgcgatcaag atttattatg tacggagtgc gtattaaaat aaaa
//
LOCUS       DAHHPA010000013        39098 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.014, whole genome shotgun sequence.
ACCESSION   DAHHPA010000013 DAHHPA010000000
VERSION     DAHHPA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.014"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(181..2562)
                     /locus_tag="NBH55_000642"
     CDS             complement(181..2562)
                     /locus_tag="NBH55_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCD1508651.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2552..3199)
                     /gene="recU"
                     /locus_tag="NBH55_000643"
     CDS             complement(2552..3199)
                     /gene="recU"
                     /locus_tag="NBH55_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCD1508652.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3263..3802
                     /locus_tag="NBH55_000644"
     CDS             3263..3802
                     /locus_tag="NBH55_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCD1508653.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3892..4311
                     /gene="gpsB"
                     /locus_tag="NBH55_000645"
     CDS             3892..4311
                     /gene="gpsB"
                     /locus_tag="NBH55_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCD1508654.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4333..4700
                     /gene="rnpB"
                     /locus_tag="NBH55_000646"
     ncRNA           4333..4700
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="NBH55_000646"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4909..6075
                     /locus_tag="NBH55_000647"
     CDS             4909..6075
                     /locus_tag="NBH55_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCD1508655.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6121..7617
                     /locus_tag="NBH55_000648"
     CDS             6121..7617
                     /locus_tag="NBH55_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCD1508656.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7698..8381
                     /locus_tag="NBH55_000649"
     CDS             7698..8381
                     /locus_tag="NBH55_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1508657.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9026..10216
                     /locus_tag="NBH55_000650"
     CDS             9026..10216
                     /locus_tag="NBH55_000650"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCD1508658.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10318..11271
                     /locus_tag="NBH55_000651"
     CDS             10318..11271
                     /locus_tag="NBH55_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCD1508659.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11340..12689
                     /locus_tag="NBH55_000652"
     CDS             11340..12689
                     /locus_tag="NBH55_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1508660.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12872..13195)
                     /locus_tag="NBH55_000653"
     CDS             complement(12872..13195)
                     /locus_tag="NBH55_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508661.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13324..14487
                     /locus_tag="NBH55_000654"
     CDS             13324..14487
                     /locus_tag="NBH55_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCD1508662.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14557..15906
                     /locus_tag="NBH55_000655"
     CDS             14557..15906
                     /locus_tag="NBH55_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1508663.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15951..16382)
                     /locus_tag="NBH55_000656"
     CDS             complement(15951..16382)
                     /locus_tag="NBH55_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1508664.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16770..19412
                     /gene="alaS"
                     /locus_tag="NBH55_000657"
     CDS             16770..19412
                     /gene="alaS"
                     /locus_tag="NBH55_000657"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCD1508665.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19612..20208
                     /locus_tag="NBH55_000658"
     CDS             19612..20208
                     /locus_tag="NBH55_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1508666.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20330..21478
                     /locus_tag="NBH55_000659"
     CDS             20330..21478
                     /locus_tag="NBH55_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD1508667.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21516..21929)
                     /locus_tag="NBH55_000660"
     CDS             complement(21516..21929)
                     /locus_tag="NBH55_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCD1508668.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22008..22439
                     /locus_tag="NBH55_000661"
     CDS             22008..22439
                     /locus_tag="NBH55_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCD1508669.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22538..22771)
                     /locus_tag="NBH55_000662"
     CDS             complement(22538..22771)
                     /locus_tag="NBH55_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCD1508670.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22954..24624
                     /locus_tag="NBH55_000663"
     CDS             22954..24624
                     /locus_tag="NBH55_000663"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCD1508671.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24771..25157
                     /locus_tag="NBH55_000664"
     CDS             24771..25157
                     /locus_tag="NBH55_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508672.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25144..25842
                     /locus_tag="NBH55_000665"
     CDS             25144..25842
                     /locus_tag="NBH55_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD1508673.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25847..26311
                     /locus_tag="NBH55_000666"
     CDS             25847..26311
                     /locus_tag="NBH55_000666"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCD1508674.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26308..27219
                     /locus_tag="NBH55_000667"
     CDS             26308..27219
                     /locus_tag="NBH55_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1508675.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27454..27993
                     /gene="pyrR"
                     /locus_tag="NBH55_000668"
     CDS             27454..27993
                     /gene="pyrR"
                     /locus_tag="NBH55_000668"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCD1508676.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28007..29290
                     /locus_tag="NBH55_000669"
     CDS             28007..29290
                     /locus_tag="NBH55_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCD1508677.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29303..30229
                     /locus_tag="NBH55_000670"
     CDS             29303..30229
                     /locus_tag="NBH55_000670"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCD1508678.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30248..31528
                     /locus_tag="NBH55_000671"
     CDS             30248..31528
                     /locus_tag="NBH55_000671"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCD1508679.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31533..32609
                     /gene="carA"
                     /locus_tag="NBH55_000672"
     CDS             31533..32609
                     /gene="carA"
                     /locus_tag="NBH55_000672"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCD1508680.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32614..35799
                     /gene="carB"
                     /locus_tag="NBH55_000673"
     CDS             32614..35799
                     /gene="carB"
                     /locus_tag="NBH55_000673"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCD1508681.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35853..36629
                     /locus_tag="NBH55_000674"
     CDS             35853..36629
                     /locus_tag="NBH55_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCD1508682.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36626..37558
                     /locus_tag="NBH55_000675"
     CDS             36626..37558
                     /locus_tag="NBH55_000675"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCD1508683.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37555..38271
                     /gene="pyrF"
                     /locus_tag="NBH55_000676"
     CDS             37555..38271
                     /gene="pyrF"
                     /locus_tag="NBH55_000676"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCD1508684.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38278..38907
                     /locus_tag="NBH55_000677"
     CDS             38278..38907
                     /locus_tag="NBH55_000677"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCD1508685.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38895..>39098
                     /locus_tag="NBH55_000678"
     CDS             38895..>39098
                     /locus_tag="NBH55_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508686.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRII"
BASE COUNT        12586 a         6916 c         8519 g        11077 t
ORIGIN      
        1 taatgataaa agaaaaatgg attttctaat ttttaagcta aatgttagat cacacatgta
       61 tatgctggat agtcttataa aaaatcatgt aatacagctt ggtacatgat ttttatttgt
      121 agctgttttt tacatagaca caaaaaagaa accacgcttg tagacgtggt ttctttttta
      181 ctatctgttt ccagaagctg aggtgctgct ggatggtgta tggtcatttg cagcatccgc
      241 tccgctagtt gctggctcac tggaagaagc aggaggagca gatgaagacg gtgtcgagct
      301 ttcttcggat gaagaagaag gaaccgttgt tgaagacgct ggctcgcttg tttctgctgt
      361 cgtgctttct tttgaggact cagcagttga ttcgctttga ctagtagagc tagagctttg
      421 agtcgttgtc tctgttgttg agcttccagg agtctggaca tagctggaag atggaatagt
      481 tgttgatggt gtaattgcat tcgatctagc tgtatattga tctttgtagt acagttcacc
      541 gcccacacga attaatccgc ttggcatctc ccagtctgta tttgtcacgt tagctgaaac
      601 atactgcata agctcgcgat aaacatcaga ggctacgtgt gacgattctg aagtgacagg
      661 tgtcattttt ttattgtaac ctgtccacac ggaaatcgaa taatttggcg tatagcccgc
      721 aaataggata tctggataca ccccagaaga aataccaagt tttgcatact cgtcatctgt
      781 gtaattggat gttccggtct taccggcttg atataaccca gcgatttgcg cattcgtacc
      841 agttccttca gtgatcgtat cttttaaaat gtctgtgatc atataagctg tttctggaga
      901 catggctgtt tttccatctg gctcataagt ttcttctgtt ccgtcttgga agacgatttt
      961 attaacatat tgaggtttgt aataagtccc tccattggcg aaagccgcat aggctgctgc
     1021 catttttaag gaagaagctc cgtattttgt cccatcttgt tcttccgtat tactagagat
     1081 cgcattcgat tgatggatcg tactgtattc gatcccaaga ttttttaaga aagaagccac
     1141 tttatctgaa ccgacttcat tgaataattt gactgctgga acattacggg ataagtaaag
     1201 tgcttggcgt aaagtaatgg tgcccatata ttgattatcc cagtttccta caggagtcga
     1261 agtcccttca tagttatacg gtgcgtcagt aatagttttt ccagtagaat atttcaaata
     1321 ttcaaaagct ggtccgtaat ctgtgacagg tttcatcgta gaaccaaaat cacgggaagt
     1381 attcactgct aagttgttgc ctaaagttac gtcttctgcg atgtgacgtc cgccaatctg
     1441 ggcttttact ttcccggtat tggtgtcgat aagggtggag gcaacttgca tctcgtcgtc
     1501 tgggtaagag acatactgat ccgtgttgac gatatcatat agtttctttt gggcgtccag
     1561 atccaaattc gtataaatct ctaaaccgtc tgtgtagaca ttcttatctg ttttttcttg
     1621 tacttcattg atgacttctt tgacataatt atcgacgatc ttagtattgt catcggattg
     1681 agttagttct tgcagaccat ccgttaccgg tacattaaca gcttgatcat attctgtttg
     1741 agagattttt tcgttttgaa gcatcgtata caaaacagta tctcgtcttt tcttcgcctg
     1801 atctggataa acataaggat cataggcgga aggggcttga ggcatcccag caagcaatgc
     1861 tgtttgcggt aaagaaagtt ctgataattt cttaccaaag tacatctcag aagctgtctc
     1921 catcccgtac aagccgtttg acatatacac tttatttacg tagtaggtca agatctcttg
     1981 tttcgatttt ttttgttcca atcttacagc catccacgct tcttgggcct tgcgtttcag
     2041 agtctgatcc tcggcactgg tggagaagaa ggaaagtttg atcaattgct gtgtcaaagt
     2101 acttccgcct tgcagtccgc cagaggtaaa gttagagagg gcagaaccga tgatccgaat
     2161 agggtcaacc ccgatgtgtt tataaaaacg gcgatcttct accgacacga tcgcatcttc
     2221 taatgttttc ggtaattcat tcgcagaaat tttttcacgc ttttctgcgc ctaaatcttc
     2281 gaatagttcg ccatcttgtg tatatagttt ggaagatacg gtagcgtcta attttttatc
     2341 cgtcaattcg ggcgcatctt ttgcgtaata ccagaaaagt cctacacctg ctaaaaagag
     2401 gatacagaaa aatgagagta ttcctaaaag gatcttgata aataatcctt ttttatgtgt
     2461 tccagaggac tttccagaac ctttgccaga attcttcttc acactctttt taggagtagg
     2521 ctgtttctgt cgtctagagc ttctagtttg ttcatttgcc attcttgctt tctccttcaa
     2581 tagataatgt tgataaatag aagtcaaccg cgtccaaata aggaatccgc ggagaaatac
     2641 cagttttgat ctctattcct agttcttcga tcaaagataa aggtagtgat ttgcgtcctt
     2701 tttccttttg ggcgtcccag taagcaatca aatgaacact attcaaataa aaacagcgct
     2761 tcaaagacga aaaccataac aagacaaagc agaccccatt ttgttcgagg catgctttca
     2821 tatgttcgat ttgatgggca tggaagtttt taaagggaaa agatgttttg ttcttggttt
     2881 cttttgcttc aaagtccaag taaaagcctc gatagacgcc attatagtct gtagttgagg
     2941 cttgggtaaa gtaggcttcc ttgatcacag cagcacttct cttaggatag tctactttca
     3001 cgatttggat aggggtaggc tttttatgga taaccgccaa cccgcgggaa agataatatt
     3061 tattactttc attgattgct tcttcaaagc gcatcccccg attgccgaat tcagtgatct
     3121 gatttctttt ttcttgaatt ttttcgacgg gagaggcatt cttataataa ggttggccat
     3181 ttggatagcg caataccatc tttaatcaca ctcctaggcc acattatagc aaaaaagcgg
     3241 atagaaagaa aaggaatctg agatggataa gtcaaaagta atctatgtgt ctgggtatcg
     3301 aagtttcgaa ctcggtgttt ttaaagaaaa tgatcctaaa atccaagttg ttaaaaatgt
     3361 tttaaaaaaa gaactccatc agttagcaga agaagggctg gaatgggtac ttgtcagtgg
     3421 gaatttgggc gtcgaactat gggctgtgca aatagtagcg gaattgaaag aagaatatcc
     3481 ggaactatct cttggtattt tgtatccttt ttctgatttt ggtaataact ggaatgaagg
     3541 gaaccaagaa aaaaaacaat tagcagaaca attagcagat tatgtcgaag ctgttagcca
     3601 tcaaccctac caatcaccta gtcagctaaa gaatcacact cgttttatac ttgagcatac
     3661 agtgggctgt ctgctgatct atgatgaaga attccctgga aaaacgaagt atttttttga
     3721 agatgcacag aattaccaag cagatcatcc ctacgaaatt cggctgatca gtatggacga
     3781 tcttcaaaat tttagtgaat aatcgatatt gtgtttgatt tatcaacttt tttcctatac
     3841 aatgaaaaag gtagaaatgt tattacgtaa atgaaaacta gaggtgtaaa tatggcgaat
     3901 ttggtttata gtcctaaaga cattttacaa caagaattca aaacaaaaat gcgcggctat
     3961 gatcccgttg aagttgatga attcttagac aatatcatca aagattatga aacttacagc
     4021 aaagaactcc ttgctttaca agaagaaaac gatcgattga gtgcaaaagt agctcaatta
     4081 tctaaaactc aaggagcagc tcaaacacgc gtgcaacaaa cagaggcacc aaagagcgca
     4141 gcagtgacca attttgatat cttaaaacgc ttgtcaaatc ttgaacgtga agtatttggt
     4201 aaaaagcttg atcaacaagc aagtgcagtc aaaccagcac aacctaatcc aaataactac
     4261 acaaatgcag acacaagctt agatgataat gaaaaaacac gtcaatttta atttttgatt
     4321 aatgaatttg cggttttcgg gtaatcgcgg tctgacttga tcaggctgag gaaagtccat
     4381 gctcgcacag gctgtgatgc ctgtagtgtt cgtgcttagc gaaaccataa gctaaggtac
     4441 tctttggagt aacggcagga aaacaagcta aggcttcggc tatgcttaag tatccttgaa
     4501 agtgccacag tgacgaagcg gctggggaaa cccatccggt ggaacgcggt aaacccctcg
     4561 agcgagcaac ccaaacaatg gtaggggcgc tttttgtctg gaagaagaac aggacaaaaa
     4621 ggcagtataa ctgcagatag atggttaccg ctttgttttt tctccctgga aaagcaaagt
     4681 acagaacatg gcttatagaa aaccgcaaac atcagggaat taggtcgtga caaaagtaat
     4741 gcgttataag aaacaagaaa aacatttgaa agctattttt caatacttca gatgaatcgt
     4801 tcttattaca aaacaaactt ttgtatcacc ggttattcgg aatttgagta aaagtgtggg
     4861 gtaggactga tgtcccacct ccttttttta taaataagga gattgtgtat ggaaaaaaac
     4921 aggaagttca acctcgtcgc aactgcggca agcgggctag aagcactggt tggtaaagaa
     4981 ttaagagatc ttggaatcga atgccaagtt gagaacggac gtgcacgttt tgaaggaaca
     5041 atggaaacga tcgctacagc taatttatgg ttacgtacag cagatcgagt aaaaatcgtg
     5101 gttggggaat tcgatgcgta tacgtttgat gaattgtttg aaaacgtaaa agcattgcct
     5161 tgggaagatt ttcttccact agatgcagct tttcctgtag ctggaaaatc gatcaagtcg
     5221 aaactatata gcacacctga ttgccaagcg atcacaaaaa aagcaatcgt tgaacgtctc
     5281 cgtacctatt atcaccgtcc agcttctgta ccactgacgg aaactggcgc acattttcag
     5341 ttggaagtag ctttgctgaa agaccatgtg atggtaactc tggatacgac aggaccaagt
     5401 ttattcaaac gtggttatcg cttagaaaaa ggtggagcac cattgaaaga aaacatggca
     5461 gcagcactta ttatgttgac aaattggcga aaagaccgtc cattttatga tcctgtatgt
     5521 ggttcaggta cgctttgtat tgaagcagcc ttaataggcc ataatatcgc accaggattc
     5581 aatcgtgagt ttgcttgcga atcttgggat tggttcagtc aagaaatcat ggaaaatgtt
     5641 cgatcagcag ccgaagaaaa agcagattat gatattgaat tagatattac tggttcagat
     5701 atcaatggac ggatgattga aattgccaaa gccaatgcag aagaaatcgg attaggctct
     5761 tcgattacct ttaagcaaca agcagtcaaa gacttcaaaa cggataaaga atacggggtc
     5821 atcgtggcta atccgcctta cggagaacgt ctaggagaag aagaaactgt tcggaaatta
     5881 tataaagaaa tgggcgatgt attccgtcca ttaaaaacat ggagtaaata tatcttgact
     5941 agcgacttag cgtttgagga attttacgga caaaaagcaa ccaaaaaaag aaaattgtac
     6001 aatggtgcat tgcgtacgga tctattccag tattggggaa ctcgtccgcc tagaaaaaaa
     6061 gagaaagacg aataatctta ctaattgatt cattcaattg gatgtttgga ggaataggga
     6121 atgaaagaaa aagagttttt acaagaagta aaagaaatca atcttctaca acaagcttta
     6181 ggaatcctcg attgggatac acaaacaggt atgcctgaaa aagctagtgc ataccgaagt
     6241 gaagtagaca gctatcttta cagcatttat ttttcaaaaa aaatcggtcc aaaaatcaaa
     6301 gaagccattc gctatttttc tgaacatccg gaagaattgt cggaagttgg aactgccgtt
     6361 tttgccaaag taaaagaaga gtatgatttg gaacatcgcg taccggaaga tcttatgaga
     6421 aaatatacag ctgccgtttc ttcagcacac ggacaatggc aaaaagctag agaaacacaa
     6481 caattttctg attttcaaca cgcattgaat caaaatattg aattaacaaa acagttgata
     6541 ccttactgga gaaaagatga aaagacgaat tatgatgttt tgctgaatca atacgaaccc
     6601 gggatgactg tcgaaatcct tgataaggta tttgatcagc ttaaagaagg aattctttcg
     6661 atccgccgta cgctccaaga aaaagggact gaacctgaaa cagacttttt aaatcggaga
     6721 atgacgaaaa aacaacagaa gagatttgta gtgaaagtaa tcgaacaact tggttatgat
     6781 tttagtcgtg gtcgtttaga tgacactgtc cacccattta tgactggaat taatccgaat
     6841 gatgcacgaa tcacgacacg atggaacgaa acagatttca aaatggctgt ttttggagtg
     6901 atccacgagg ctggtcatgg tatgtatgaa caagacatcg atgataaata tgcgtataca
     6961 ccggtttatg aaggtacatc aatgggtatc catgaatcac aatcgttgtt taacgagatc
     7021 attattggaa gtagtcgcag cttctggaaa aagcaatatc ctttctttca agaatgtgca
     7081 gaaggaacat tcgatgatat tccttttgac aaattttatc gttcattgaa ccatacaaaa
     7141 tcaagtttgg ttcgtatcga ggcagacagt ttgacttacc ctctccatat catcattcgt
     7201 tatgaaatcg aaaaaatgat tttcaatgaa ggggtagatg ttgctgaact acctgaaata
     7261 tggaatcaaa aatatgaaga gtatctggga atacgaccaa caaatgatgt ggaaggcatc
     7321 ctacaagacg ttcattggtc aggtgcaagc tttggttatt ttccctctta tgcattaggg
     7381 tacatgtatg cggcacaatt acttcattct ttgcagcaag aaatagatgt ggaaaatgtt
     7441 ctagcgtcag acgattattc tccaatcaaa aactggatgt cagagcatat ccatcaatat
     7501 ggtgcatcta gaaaaccaaa tcaattaatc atggatgcaa cacatgaacc gcttaatccg
     7561 caatacctga tcgactatat gaaagacctg tattttaatg tatatcaggt aaaataaaat
     7621 acaatttaca aagatagctg aaattcatct aatctataga tgttttcagc tatttactta
     7681 agaaagaagt ggaacgaatg gataaattac aagtgatcgc tgattatagt atggaccaat
     7741 tagcgcatga tcaaacagga catgggagcg atcataccaa aagagttgtc aagttagctg
     7801 aaaaaatttt ggagacggaa ccgcaggcag accgctttgt gacgcttgca gcagcttacc
     7861 tacatgatac gattgatgat aaagtcgtta aaaatgagaa cgaggcaaag caacagcttc
     7921 gtgtattcct tcgctctttg ccaatagtag aggaacagat ctcgatgatt ttttccatta
     7981 tcgaaaatat gtccttttct aaaaatctct ctgaagcggc cgaactatct ctggaaggta
     8041 agatcgttca agacgctgat cgtatcgaag cattaggagc aatcggcatt ttgcgtactg
     8101 cttatttcgg cggtggacat gggcacccga tttttgatcc tgaactgtat ccgcaaacat
     8161 tcaaggacaa agaaaattac cgaaaaggca cgacagtgat caatcatttt tatgaaaaac
     8221 tgttcttgct gtctgataaa atgaatactg attacggata tgaagaagcc aaacgaagag
     8281 aaaccttcat gcgtgaattc ttagaagaat tttttctgga atggtctatt gacgatgaaa
     8341 attttactcc agacaattgg atcaaaaata acaaaaaatg atggaggttg tgacagaagt
     8401 ggtcagctcc aagatatttc ggatcatttt ccgtaaaagt tacttctgga acactgttta
     8461 ccaagaggtc gtgagcctgc cgttcagctc cgagtcacaa ggaacaattt caaaaaatag
     8521 cttctcgtgt ttgatgaata ttgtaacttg tgattcggaa ttggcaggag aacaccgttt
     8581 attcggaaga ataggactgt gacaagactt ttgtcacagt ccttttttcg aattcaacaa
     8641 gtaaaacttt taagaaatat tcagagagta aaattaaaaa ttgctgataa acgaagatgg
     8701 aaattttatg tatttttatt ggtctatgca ataaataaat taaatattca gaatacaatt
     8761 gattttaacg aaagggcttc tatcaatggt tgtgttgtat ttatctgccc aggtagagta
     8821 aaatagatca ttatctaaaa ggtaattgtt catactatat aagtcatttg aaagtaaaaa
     8881 gatactgtaa ataaaaaatg tggttaaata attctcataa tcagtgtaag cgttatcaaa
     8941 aatatagcat acttgctttg tagatgaatg acacgatata tgtttggtat gttctgcaag
     9001 acagatgaat atggaggtgg agacaatgtc ttccaaagaa ctaacaatgt atttagagaa
     9061 agaattggat caactgaaag aaaaggggtt atacaatacg atcgacatac tagaaagtga
     9121 aaatggtgct tgtatcatcg tagacggaaa aaagatgatc aatctagcat ccaacaatta
     9181 tcttggattt gccaatcgcg aagaattgaa aaaagcatgt attgaagcaa ccgaaactta
     9241 tggtgtagga gcgggagcag tccgaacgat caatggttcg ctgaaaatcc atcagcaatt
     9301 agaagaaaaa atcgcagaat tcaaagggac agaagcagca atcgcctttc aatctggatt
     9361 caactgcaat atgggcgcta tctctgcagt aatgacaaaa gaagatgcta tcttatcgga
     9421 tgaattaaac catgcatcta ttattgacgg ttgccgctta tccggagcaa aaatcatccg
     9481 aattaaacat caagatatga aagaccttga gaaaaaagca aaggaagcga tagaaagtaa
     9541 gaaatataaa aaaatcatgt atatcacaga tggtgttttc tccatggatg gagacattgc
     9601 acgattgccg gaaattattc cgattgttga aaaatatgga ctgatcacct acgtcgatga
     9661 tgcgcacggc tcaggcgtaa ctggaaaagg tgcaggaacc gtcaagcatt ttggattaag
     9721 cgacaaaatc gatatgcaaa tgggcacgtt atcgaaagct ataggtgttg ttgggggtta
     9781 tgtagctgga tcgaaaacac taatcgactg gttgaaagct agaagtcggc cattcctatt
     9841 ttctacttcg ctaactccag gtgcagctgc agctgcattg gcttcgatta ctttgatgca
     9901 agaacatccg gaacttgtcg aaaaagtttg ggaaaatgct aattatttta aagaagaatt
     9961 gaaaaaagta ggctataaca tcggtatgtc tgaaacacct attactccag tcattttggg
    10021 agacgaaaaa gtgactcaga ctttctctaa gaagctgatc gaacatggga tttatgctaa
    10081 accaatcgtt tatccaactg tgcctcttgg gacaggcagg atcagaaata tgccgactgc
    10141 ggaacatacg agggaaatgc ttgatgaagc agtagcagtt tatcaaaaaa taggaaaaga
    10201 aatggaaatt atttaactaa atttagtttt ttcgtctatt taaaattaga gaacatgttc
    10261 acgcatatat tgttaatcat ctgaagatag tagtaaaaaa gatcagggag gatcatcatg
    10321 aaaaaagtat tggtgacagg atgtttagga caaattggtt cagagttgat cacacggcta
    10381 cgtcaagaac taggcagtga aaatgtcatt gcgacagata tacgcatgcc tgaagtcatt
    10441 gagaaaaacg atatctttga gatcctagat gtaatggatt acgaaaaaat gcgcagcata
    10501 gcagaacaat atcaagtgga cacattgatt catttggctg ctcttttgtc agctgttgca
    10561 gaagcaaaac cacaattagc ctggaatttg aacgtgactg ggctggtcaa tgcattggaa
    10621 gtcgctagag aattccagct gaaatttttt acgccaagtt caatcggcgc gtttggtcca
    10681 aatactccaa aagatcatac cccacaagat actcttcagc gtcctacaac catgtatggg
    10741 gtaacgaagg tagctggaga actactatgt gattattatt atgtgaaata tggcgtagat
    10801 actcgtggtg tccgtttccc tggattgatt tcttatgcaa ccttgccagg aggcggtacg
    10861 acagactacg cagtcgatat ttattattca gcaatcaaag aaggaaagta cactagtttc
    10921 attgatcaag gtacatcaat ggatatgatg tatatgccag atgcgatcga tgccattata
    10981 aaactcatga atgcagatcc tgatcgattg gttcatcgaa atgccttcaa tatatcggcg
    11041 atgtcttttg acccggaaga gatcaaagca tctattcaaa aaatcatacc agattttgaa
    11101 atggattatg atgtggatcc tgtacgtcag tcaatagcag aatcttggcc aaattcacta
    11161 gatgtttctt gtgcgcaaga ggaatggggc ttttcgccga aatacgactt agatgtgatg
    11221 acaaaggata tgttggaaaa actgaaagaa aaattggtag agacggtata aaacaagaac
    11281 cacatccaaa aagtttttta tacttttggg attggctatg gtatgaaagg ggacaaggga
    11341 tggaagaata tcaagccacg ccagtaaaag aagtaaaaat agaaaatgaa ttaaaacgta
    11401 cgatgggatt ttttactgct ttatcgactg ttatgggcac agtgatcggt gcaggggtat
    11461 tttttaaagc cgcaagcgta gcagaagtta caggatccgc cagtttgcat atgttttctt
    11521 ggtttctcgg tggtatgatt tctgtctgtg caggactaac aggagctgaa ctggcagctg
    11581 ctattccaga aacaggaggg atgattaaat atatcgaacg gatttacgga aatacagcag
    11641 cttttttatt gggctgggcg caagtcgtga tttattttcc agcaaatgta gcagccttgt
    11701 cgatcatttt cggtacacag ttcgtcaatc tatttggtct ttcccagtca atgattgttc
    11761 ccgtagctgt tacagctgct gtatcgatcc tgttgatcaa ttttttaggt tctaaagctg
    11821 gaggggcttt tcagtcgatt acgcttgtct gcaagcttat tcctttgttt gtgatcgtca
    11881 ttttcggttt gttccgacaa gaaggagtag acttccaact gtttccgatc caagcaggag
    11941 aaaatctttc atttttctca gccttgggag cgggcttgct agcaacgatg ttcgcatacg
    12001 acggttggat acacgtagga aatatttcgg gagaattgaa aaagccggcg aaagatttgc
    12061 caaaagcaat ttctttagga atcatcggaa tcatgatcgt ttatttatta gtaaatgcgg
    12121 tgttcttaag aaccgcatct atcgacggag tcgcaggaaa cagcaatgcg gcaagtgacg
    12181 ttgcaaaaat gatttttggc ggcttcggtg gaagactggt cacagttggt atcttgattt
    12241 ctgtttatgg aaccatcaat ggctataccc ttactggaat gcgtctccct tatgttatgg
    12301 caaaagaaaa caacctgcct tttagcaagc tctttgctaa acttcatgat aaaactaaag
    12361 ttcctgtagc cgcaggtatc ttagagctag tcatcgctat tggcatgatg atgatcggtg
    12421 gttttgatac actaacggat atgctgatct ttgtcatttg gattttttat acgatggtct
    12481 ttgttggtgt cattcttctt cgtaaaaaag aacctgattt gtttagacca tacaaagttc
    12541 caatgtatcc gtttattcca ctagtagcaa tcattggagg gacttttatc gtatcaagca
    12601 cgcttatcac tcagaccttt ttagcttcga tggggatagt attgacactt gctggtatcc
    12661 cgatttatct ctatttaaaa cgtagatagc taaaacattc gaattttatt agaactgcac
    12721 ttctctgcca aacaaaatat tttatttaaa tgaggagtac gtggaagaaa tgtcaaaagt
    12781 actcgttaat tttgatcacg taaatgtcgt tttgtctacg atgaaatagc aaagtaaaag
    12841 acagaaatcg ggctcaaccg atttctgtct tttactttga tttgcgtaac tgaactattc
    12901 ggcgtacgcc gcgataaata tttaataaaa ccaagcaacc agaaatgaaa atgggaagcc
    12961 aaatcagata gacagggtag ccaagcactt tagacaaagc atgtggagtc attttatcca
    13021 tttcaagaag tgcttgctgc caagtctgaa gattgaagac tagcatgaaa cccgaaataa
    13081 tcaggagaat aaatcctaaa taatgaattg tagttctttt tcttacataa gcgatcgtag
    13141 cataaaggaa cagtaaaaaa ataggtacgc caaaaacaaa gtacatattc agcatctcga
    13201 cacccccttt gtgtttatct tactttactt tgaaagaaat gagaaataat agcctcgtta
    13261 cttgcttgaa acagcaaagg actgctaaga taagagagat acaaagaatc aaggaggagt
    13321 catatggcaa gttatatcga accgattcgt tttgcgctga ttgtttttcc atttttagcc
    13381 ttagcgatct catctgtttt ttttatttat gaatatcgaa agtatggaac atttctagta
    13441 acaagggcag ttattttgta ttctttcgtt ttttatttat tgtgtgcgta ttttttggtg
    13501 atcttaccac ttccggcaaa ggagacggtt gcccaaatga cagggccaaa gatggagctg
    13561 catttatttg cttcatggca acatttcaga gacaacacgg tattggtctt gtctgaacct
    13621 agtacctacc tgccggcaat gaagcagagt gtatttttgg aaccagcttt taatgtttta
    13681 ttactcatac catttggcat ttatttaaga tattatttcc ggctttcttt tatgaaaacg
    13741 atgattgcta gtttctgcct ttctctattt tttgaattga cgcagctaag tggactttat
    13801 tttatctacc caagaccata ccgcttattt gatgtgaatg atttagtgac aaatacattg
    13861 ggtggaatca ttggttttgt cgttacgccg ttatttacat ttatgcttcc tacaagaaga
    13921 cgaatggatg aactttctta tgaaaaagga aagactgtct cattgactcg acgcttggtc
    13981 gcttatttga tcgactggac atttttatct cttattaatt tggtagcggc gattttcttt
    14041 aaaattgcgt tcgattcagg ggcagttact tctcatatct ggtggctttt attagaagta
    14101 ggggtatact ttatgattgt accgtttata accaatggat ggacacttgg caaaaaaatt
    14161 gtgcgtatcc gtttggtgga agaagaccgc gaacgaattt ctttaaaagc attgatgatc
    14221 cgctacgggt atctgtactt tcttttttat ggtttaagct ggttggggca gttgcttcag
    14281 caaatcatga attcgacaac gaatacggtg ttgcagatga gtgcagtatt catttctctc
    14341 ggtgtcttgt tactacaagg tatttttatt ttaaatatct tgctatcatt tttgagaaaa
    14401 aaacggattc tgttttatga aaaagctagt cacacatata ctgttagcac gattggccaa
    14461 acaaaggatg atgattctcg aaattagctg agcaaaaacg ctttgtctgt gttataatgc
    14521 caaagtcgtg ataatttaaa gagaagaggt gcacgaatga ataattttga tatattccgc
    14581 ttcaaaccgt ttatcaatga agcgttaacc gcaaagggct tcaaacagcc cacagaggtc
    14641 caggaacgtt tgattccttt aattgcaaaa ggaaaaaata tcattgggca atctcaaact
    14701 ggtacaggaa aaacacatac gtttttgttg cctttgtttg aaaaaatcaa tgcagaaaaa
    14761 gaggaagtac aagttgttat tacagctcca agtagggaac tagctgcaca gatctatgaa
    14821 gcagctacac aaatcgctaa attttctaca ccggagatcc gtgtctcgaa ttttgtcggc
    14881 ggtacagaca aacagcgtca attaaatcgt ctgcatcacc aacagccaca ggttgtagta
    14941 ggcacgcctg gtagaatatt agacatgatg aatgaacaag cgctgaagat tcacaccgca
    15001 tttgcatttg ttgtcgatga agcggatatg acattggata tgggtttttt aaatgaagta
    15061 gaccaaattg caagtcgatt accagaaaag ctgcagtttt tagttttttc tgccactatt
    15121 ccagaaaaac ttcgtccatt cttacgaaaa tacatggaaa atccaatcat tgaagaaatc
    15181 aaaccaaaag cagttatttc tgaagacatt gaaaattggc tgatttcaac aaaaggtaaa
    15241 gatgagaatc aaatgattta tcaactgctg acaattggtc atccttattt agcaatcgta
    15301 tttgccaata caaaacagca tgtggatgaa attgccgatt acttgaaaaa ccaaggcttg
    15361 aaagtagcaa agattcatgg agacatcacc ccaagagaga gaaaacgagt gatgcgacaa
    15421 gtacagaatc tggagtatca atatgttgta gcgactgatt tagctgctag agggatcgat
    15481 atcgaaggtg tctctcatgt tattaatgca gaaattccag aagatctaga cttctttatc
    15541 catcgtgtcg gacgtactgg acgtaatggc ttaaaaggaa cggccattac gttatattcg
    15601 ccaaatgatg agcaagccat tgatgcgata gaaaaattgg gcgtctcatt tgaaccaaaa
    15661 gaaatcaaaa acggggaaat cgtcaaaaca tacgatcgta atagacgtgc caaacgagaa
    15721 aaatcaaaag atgtattaga tcccacgctg attggtcttg tgaagaagaa aaagaaaaag
    15781 atcaagcctg gttataagaa aaaaatcgat tgggcaattg cagaaaaaaa taaaaaagaa
    15841 cgcaaaatcg aacgcagaca gcaaagccgt actgctcgta aaaataagaa aaattcacat
    15901 aaataaaaaa caagaggttg tgacaaaagg cgtgtcacaa tctcttgttt ttaagctaga
    15961 ctttttttca ttccgataca aagtacttca tcttcaataa aaggttctgt gcaagtatga
    16021 tacccttgtt tttcataaaa atctacagcc gtcatttcag ctactacata actagtctga
    16081 caaccttctg aaaccgcccg tttttcagca gcttcaagca actgaccgcc taaccctttt
    16141 cctcggtatt cttttgctac gcaaaatcgt tccggcttga agcaagtagg tgttttcttt
    16201 tcataacgca aagtagctac tggtcttttt tgatcaagaa atagataata ggtttgttgc
    16261 gggatatctt ttccgtcgaa ttctagttct gcaggaatcc cttgttctaa tacaaaaact
    16321 tgatatctca aaaagcagct agctgcgcgt atccattcat ccgctccaac tatttttttc
    16381 atcatattcc tccttgtcat gaatagcaga gatgtattga ttcctttatt cttatcgtac
    16441 caaaaatcgt ggagtatgac caaaaggaaa tgaaaataaa aattgagata ttgacagtga
    16501 tcaatagaat gcgtatacta attaagtacg aaaaggatat gatttgtttt ttaagactca
    16561 cagaaaattc tttcttggct gaaaaaagaa tagtcactaa tcaaattgct ccctttttct
    16621 attttggtag aaataccgac gcagctcagc gttatcgagt ttaagaggta atgattacac
    16681 atcattgcaa tcaaggtggt accgcgagtt ttcgtccttg gctgtgatgg tgtttttttg
    16741 tatttaattt atagaaagca ggtaatgaca tgaaacaatt aagtagtgca caagttcgac
    16801 aaatgtttct tgatttcttc caatctaaag gacatacgat tgaacctagt gcgtctttga
    16861 tccccgtgaa cgatccaacc ttattgtgga tcaactctgg tgttgcaaca ttgaaaaaat
    16921 attttgatgg ttcagttgtg ccagaaaatc caagaatcac aaatgcccaa aaatctattc
    16981 gaacaaatga tatcgaaaat gtcggtaaaa cagcacggca ccatacgatg tttgagatgc
    17041 tgggtaattt ttcgattggt gactatttca aaaatgaagc catccactgg gcatgggaat
    17101 ttttgactga tccaaaatgg atggcattcg atccagaaaa actttatgta acagtttatc
    17161 caaaagatac agaagcaaaa cgcatctggc atgaagaagt tggcctgcca ctagatcaca
    17221 ttattgatgt agaagacaac ttctgggata tcggtgcagg cccatgtggt ccggatacgg
    17281 aaattttcta tgaccgtgga gaagcttata acgatttagc agaagacgat cctgaaaact
    17341 atccaggagg agaaaatgaa cgttatttag aaatctggaa cttagtattt tcagaattca
    17401 accataagcc tgataacaca tttgaaccac ttcctcataa aaacattgat acggggatgg
    17461 gattggaacg catggtctca atcgttcaag atgcgtcaac gaactttgag acagacttgt
    17521 ttatgcctat tatccacgcc gtagaaaagc taagcaaaca agtaaaatat ggtgaaaacc
    17581 cagtaacaga tatttcttac aaagtaatcg cagatcatat tcgcgccctt tcatttgcta
    17641 ttggcgacgg tgcattgcca tcgaatgaag ggagagggta tgtcttacgc cgtttgcttc
    17701 gtcgtgctgt catgcatggg aaaaaattag gtattgaaca agcattctta tatcgtttag
    17761 tccctgttgt tggggaaatc atggtaagtt attatcctga agtacttcaa caaaaagaat
    17821 ttattgaaaa agttatccgt acagaagaag aacgtttcaa tgaaacaatc aatgaaggtt
    17881 tggaaatctt aaatcaagtg atccaagaag taaaaaacga aaacgggacg actctagcag
    17941 gaaaagacat cttcaaacta tacgacacat atggcttccc ggtagaatta acggaagagg
    18001 tggcagaaga tgaaggactg aaagttgatc atgctggttt tgaaaaagaa atggatgcac
    18061 aacgcgaacg tgctcgctct gcccgcagta ctgaaaaatc aatgggggta caatctgctt
    18121 tattgacaga tatcaaagta gaaagtaaat tcattggata cacagaacaa gaatcagata
    18181 gtcaattatt ggtcatcgtt aaaggtgagt ctcttttgaa tgaagtcgca gaaggtgaag
    18241 ctcaaatgat ctttgatcaa acacctttct atgcagaaat gggtgggcaa gtagcggatc
    18301 aaggatggat catgaatgaa gtaggcgaaa tcgtagcagt tgttacagat gttcaaaaag
    18361 cgcctaatgg tcaatttatg cacaccgtag aagtaaaagc accaatgagt gaaggacaaa
    18421 cctatcatct attgatcgat caatcgatgc gcaatcggat catcaaaaac catacagcaa
    18481 cccatctact gcacaaggca ttgaaagaag tacttggcga gcatgcaaat caagctggtt
    18541 ctttagtcgc tcctggacat ttacgattcg actttaccca ctttggtcaa atcacttcag
    18601 atgaattagc tgaaatggaa agaatcgtca acgaaaaaat ctgggaagct atcccagttg
    18661 aaacaattga aacagatatc gatacagcca aaaacatggg tgcaatggct ttgtttggtg
    18721 aaaaatacgg acatgacgtt cgagtagtaa atattggcgg ctggtcaatc gaactgtgcg
    18781 gaggcacaca tgtagcaaat acagaaaaca tcggaatctt caaaatcgtt tctgaatcag
    18841 gaattggtgc aggcgtgcga cgaatcgagg ctgtaacaag taaagaagcg tacgaactac
    18901 ttgaagaaga agaaaaacaa ctaaaacaag tagcaacaat tgttaaatca cctcaattga
    18961 aagaagttgt ttcaaaagcg gaacaattgc aacagcaagt aagagagcta caaaaggaaa
    19021 atgaacaatt agctagcaag ttagcgaatc aacaagctgg tgatgtattc aaagatccag
    19081 aaacgatcaa tggaaaaaca atcattgcag cacaagtaaa agtgaaagat atgaatcagc
    19141 tgcgtcaatt agctgaccaa tggaaacaaa aaaatatttc agatattctt gttttagcaa
    19201 acgaacaaga aggaaaagtc agtctgctag cggcaatgac gaaagaatca aatgaagctg
    19261 gactaaaagc tggcgacttg attaaagcaa tcgcaccgaa agtaggcggt ggcggtggtg
    19321 gacgtccaga catggcacaa gctggcggga aaaatcctgc tggtattcca gatgcactta
    19381 aagctgcgaa agaatgggtt gcgcaacaat agggcgttta cgtttgacag aaaaacaaaa
    19441 gttcaaacat tgaacgaaac atgattatgg gggcgaagac aaacaagtct tgccctcata
    19501 cttttttcaa ttggatggcg catctaatca aatggtcgga ttcggatggt ctttagttga
    19561 cgaaagtgac agaaattttt acgattagta taaataaaaa ggggtgaaaa aatgaatagt
    19621 agaaataaga cctttcgact agtcttacgt gcgatcttgt tagcaatcat catcgtacaa
    19681 gcgatggttc cttggctggg gtttattcca ttaggattca ttagtttaac aattatccat
    19741 atcacagtaa ttattgctgc agtagtatta ggcccaaaag atggcatggt gattggcttg
    19801 ttttggggaa tcgctacaat tgttcgtgcc tatgccatgc caacaacgcc atttgataca
    19861 ttagtgttca cgaatccgat tatctcggta gttcccagag tacttgttgg cttagtagca
    19921 ggcttggttt tccactggat ttatcaaaaa aataaatcaa taacgatcag ctcagccttt
    19981 gcaggggtac tgggttcatt ggtcaataca gtcttagtgt taggattcat gggattattt
    20041 tatacaggtg caacggcaga tgcctatggt gtagactctg ctcttttatt taaaacctta
    20101 gcaggggttg cagcaatcaa tgggatacca gaagcaatag gtgcgggaat cataacacca
    20161 ttactagcaa aagcgttatt tgcagcaaca ccgttgaaac cagaataatg accatctttt
    20221 aatagtaata ggacgcacga tagatagaaa aaggaagaga ggagaggaaa acaatcgtcc
    20281 tctccatctt tttacctgag caacaagcaa actagaaggg gtaaaaaact tggctaagaa
    20341 acaagaagaa aaaaagaaac aaataccgca acagcggaca aaaaaaggag tactagcggg
    20401 tatcttagga ttggttacga atctgatctt gtttgtctct aagctaatga tcgggctttt
    20461 ttcaggaagt gtctccatta tggctgatgc agtgaacagt ctatcagata ctgcttcatc
    20521 catattgact ttgatcggat ttaaaattgc tgcaaagcca gcagatcagg aacatcctta
    20581 tggacacgaa cgcttcgaat atatcagtgg gttatttgtt tcgatcatca ttacttatgt
    20641 aggatttcaa tttttagagt cttctgctga aaaaatcatc catccagaaa atgtctcttt
    20701 aacacccatc gtcttttttg tcctaatcat ttccatcttt ttgaaatttg cacaaggcaa
    20761 aatgtatcag gttattgcgc aatccattga ttctgaaaca ttgagagcta cagcaacaga
    20821 tagttataat gatgtcttta ctacactggc tgtattgctt tcagcagcaa tcgaatgggt
    20881 aactggatgg cgtattgatg gatatatagg ttttgcttta gcattatata tcctttatag
    20941 tggattgacg atgcttcgaa attttgtata cgaactatta ggaagtcgcc caacgccagc
    21001 agagattgaa gaaatggaag agcatttaaa tgcttgcgat gatatacttg gctatcatga
    21061 tttattggtg cataactatg gacccaataa acgctttgca tcagtccaca tcgaagtaga
    21121 tgaatcaatg aatttgaatg tggctcataa gattattgat ctgatagaaa aagactttaa
    21181 aaagtctttg gatgtggaac ttgtctgtca cttggatcca atgcctgttg ataacgaaaa
    21241 atactataat atcttgcggc agttaaagca aattcttctt gaaacaggta cagggcttaa
    21301 tattcatgat tttagagtaa ttcatcaagg aaaaacgctg caatttgatg tagtggtccc
    21361 tgagaaattc cagtttgttg atacagaact agaaagaatc ttacgtagtc aaatagaaga
    21421 aaaaatcgga gattaccaat tggaaattac gtttgatcac aattatcttt tatattaaat
    21481 gagaaactat aaaaagactc cttaaagttt ttaatttaaa gagtcttttt ttcttttaat
    21541 tgtttatgga gagcctgacg tgcaagatta tctgctcctt tatttttgtt ttcgggcaac
    21601 cattgcagga tcaataaaga aaattcggat agaagttctt ggattctttg aaggtaggga
    21661 aggaagagct cgttcttcgt tgcgtttttg tcgaccgtag aaactagtgt tttgctgtct
    21721 gaaaaacaga aaatcgtttc attctgtaag tcgtttttct ttagaaattc taaagttttt
    21781 aaaaagactt cgaactctgc ttgatggttt gtccctatag aaagcgggat agatagctgt
    21841 tcttgtttac cttgatagac aatcaagatt ccacctccac taggaccagg gttgcctttt
    21901 gtagaggcat caatgtatac ttttaacata aaaagagatt ctcctttttg ttcaaactaa
    21961 tgttatggta agattatatc attactgaat cggtgagagg ttagagaatg aaaaaaaata
    22021 ttcgatggca gcctgaactg gcacatacga tcatctattg gtcattgacc ttgatcattc
    22081 tgttttttag tctgattctt tctttagaaa acacaggtcc ttattggaaa agcaattttg
    22141 ttttaggtat ttttcttgtt tttctatttc taggtttgcg gcgttctttt attttaaaat
    22201 caaactctgt gattatacgt tatgctgctt tttggaaaga ccgagaaata tctgtcggac
    22261 agattcgatc tttgacgcca actaatactg gggtgcaaat gatgttgcaa gatgagaaag
    22321 ccccatctgt tttcatgatg cgaaaaaaag atcaagtagt cctttttgat cattttgaaa
    22381 aaaacggacc caaatcagaa tcaattcctt atccttatac gaaaaaaaga aaaagctaat
    22441 tttaaaaatc agaacctaaa aaaacgaatt tttacaggaa aagaaaagag tgagacgtac
    22501 ggttcatccc gtttagcgtc tcactctatt tgtttatcta ttcatgcata ctagtatcgg
    22561 tagtctgaac gttgcctttc ttgacatggg cagcttgtgt tcctcgattc ccttcgatga
    22621 tctcaaattc gaccacttga ttttcttcca aagttttgaa tccctcttct tcaatcgcag
    22681 taaaatgtac aaaaatttct tctgtgtcat cataactgat aaagccgtat ccttttttgt
    22741 tgtcaaacca ttttacaatt cctgtggtca aagcactcac gacctttcta aagtctgatc
    22801 atagccttca ttataaagag tatttataag tacgtcaaat aaattaaaaa cctgtaattt
    22861 tatgtttttt ttctgaataa aaaaaggtat aattggcata tcttaacaaa gaagatattt
    22921 tgttgaataa agttcgtgaa aaggggtaat caaatgaaga atgatctgca aatttcacaa
    22981 gaaacagagc tagaaccgat tgttgaagtg gctagtaaat tagatcttaa ggaagacgat
    23041 ttggaactgt atgggaaata taaggcgaaa atcaattttt ctggaatcaa tcgatcaaag
    23101 gataacgctg aggggcattt gatcttagtt acttccatca atccaacacc agctggcgaa
    23161 ggaaagtcaa cgatcactgt agggttagga gacgcgctca ataaaatcaa taaaaaatct
    23221 gttattgcat tgcgagaacc ctcacttggt cctgtgatgg gaatcaaagg tggtgctgca
    23281 ggcggaggat atgcacaggt attgcctatg gaagatatca acttgcattt tactggtgac
    23341 atgcatgcta tcacaacagc taataatgca ttatctgctc ttttagataa tcatattcat
    23401 caaggaaatg aattgaacat cgatgcccgc cgagtgattt ggaaacgggt agtcgatctc
    23461 aatgatcgtg aactgcgtaa agtaattgtt ggcttgggtg ggccaatcca aggtgttcca
    23521 agagaagatg ggtttgatat cacagttgcc agcgaaatca tggctatctt gtgtcttgcg
    23581 gcagaccttg aagatttaaa ggctcgtctt gcaaggatcg ttgtcggcta tacatttgac
    23641 cgtcagcctg tgactgctgg agacttgaaa gcagaaggcg ctctggcatt actgctgaaa
    23701 gatgcaatca agccgaatct tgtacaaaca atttacggaa caccagcttt tgtccacggg
    23761 ggaccgtttg ccaatatcgc tcatggctgc aatagtattt tagctaccaa gacagcattg
    23821 agattagcag attacgttgt cacagaggca ggatttggtg cagatcttgg cggagagaaa
    23881 tttttagata tcaaagtacc taacttgaaa aaagcgccgg atgctgtggt gattgtcgcc
    23941 actgtccgtg cattgaaaat gcatggcgga atgaaaaaag atgaacttaa aaatgagaat
    24001 cttgatgctt tgaaagtagg ttttgcaaat ttaaagcgcc atatacgtaa tatggaacaa
    24061 tatcagctac ctgtgatcgt cgcaatcaat gaattcgtaa cagatacaga caatgaactc
    24121 acactgttgg agcacttgtg tgaaaatcag gggattcttg ccaagagagc gagtgtatgg
    24181 gcagatggag cagaaggcgg cgttgattta gcaaaagctg tagtacgatt gattgataga
    24241 aaagaagcta attacaagcc attataccgc ctagaagaaa cgatacaaga aaagacagaa
    24301 acaatcgtca agaaaattta cggaggaaat ggtgttgttt tcagcaaaaa agcgcaaaaa
    24361 caaatagaag agtttacgaa gaatggatgg gacaaactgc cgatttgtat ggcaaaaaca
    24421 cagtattcat tttctgatga tccttcactg ctaggtgcac cggaagattt caccatcacg
    24481 atccgcgaaa tcatcccaaa attaggggca ggatttttag tagccttgac tggagatgtt
    24541 atgacgatgc ctggtttgcc taaaaaacct gctgcattga atatggacgt gacaaatgac
    24601 ggagaagtgt tgggattgtt ttaagcttga ttataatcta ccatcgaagg agctgtattt
    24661 tgactacagc ttcttttttt agacgatagg gaattaattt tgtttacact aggatcattc
    24721 tttttcttta ttgtattttg agctatacta aagcataata ggagggtatg atgaaaacga
    24781 catttataag aaaaaccggt ttttacggta tgggtagctc gattcagcta agaatcaaca
    24841 atgaaaaacg cagtgttctc agtcatgaac aaaagataac gcttgatctg gaagtgccct
    24901 ttacgatgca ggtcaccttt tattggctaa aaagccccgt ttatacagtg aaagaagcaa
    24961 agcctgctta tgtgatcaca atgaattact tgattcttca aatctatccg tttttatttc
    25021 tagcaactgg gttgggggcc gttttcctac aaaatttatt tttcagtttt tttgcagtta
    25081 ttgtgttgat tgcgttcttt ttctatataa aaaaccgggc atatttgatc aaggaggaaa
    25141 attatggcaa actctgatct ttttttaatc agtttcaatc gaatcgaaaa atggatgaga
    25201 gacgagatgg gaaatgcacg aaatatggga tttaccgaac ttgtcagacg cctagctcaa
    25261 agaaaacaat taatgatcag aaaatacgaa gatgatcttt tacagcttgc tcagttaagg
    25321 aatgcgatcg tccatgatcg gatcgcagtc gattttgtca ttgcagagcc aaatgattgg
    25381 acagtcaagc gaatccagct gatcgaacaa gaactgattc agcctgaaac cgtgctgcct
    25441 cgatttgcaa aacgtgtaac aggttttgaa caagatcttc cgttacttga actgttaaag
    25501 atagtagcag aaaaaaggta ttcacaattt ccgttataca ataaaggaaa atttgtagca
    25561 ttgattactt tacgtaatat cgggttttgg ctagcaaaag aaagccaaaa aggtccggtt
    25621 gatttaacaa ataaacgagc gttggatttg atcatccaaa acggaaagta cacaaattat
    25681 cattttgttc cagctaaaac acatatttac gaggttgaag cgatgtttcg tgagcaaggg
    25741 actttggaag ccgtacttat cactaaagat gggaatcccg atggtaatct gctcgggatc
    25801 gtacgacctc gtgatattta taaacaaata gaaaaggatt gattttttgt taattattta
    25861 ttggatattc agcgctttgc tagtaggagt ggatcagttg gtcaaatggt ggatcactga
    25921 taatctagcg cttggagaga cgaaaaatct tataccaggt attttgtcac tgaatcatat
    25981 tcgaaacact ggagccgcat ggagtatgct agaagggaag atgtggttct ttactatagt
    26041 gacactcatt gcagtcgttg tgattctcac attgatgatt aaaaatcgtg aaaaaggtaa
    26101 tcgctggttc atgattggat tgagtttgat tctagctggt gcgattggca attttatcga
    26161 ccgtattcgt cttggctatg tagtagatat gttccagaca gattttatga gctttccgat
    26221 ttttaacgta gcagatgtga cacttgtatg tggagtgatt tgtatgttaa tttatattat
    26281 tttagatgaa aaggatcaaa gaaaaaaatg acagaactta aaacgacgat tcaccatgaa
    26341 tctggtagga tcgataaagt attgactaat ttattcaccg attacagtcg ttcgcaaatt
    26401 cagctgtggc tgaaaaatgg agcggtttct gtcaatggac agccagtcaa agcaaattac
    26461 aaagtaaaac aaggagacga gattactgtc ttgatccccg aaccagaaac attagatgtg
    26521 gtcccagaaa atatcccgtt gacgatcgtt tacgaagatg aagatgtagc ggtgataaac
    26581 aaacctcaag gaatggtcgt tcatccttca gcaggtcata cgcatggcac aatggtcaat
    26641 gcgctgatgt accatatcaa ggatctttct acgatcaacg gcgttattcg tccaggtatt
    26701 gttcaccgaa tcgacaaaga tacttccgga ctgctaatgg ttgccaaaaa tgaccaggca
    26761 cacgaatcat tagctcagca attaaaagat aagacctctt tgcgcaaata tgttgcttta
    26821 gtccatggag agattcctca cgaaaaagga accatcaatg cgccaatcgg ccgttcgaag
    26881 gaaaaccgga aaatgcaggc gataagagaa gatgggaagt cggctgtcac acattttact
    26941 gtattggaac gtttcgaagg atttactctg gttgaattaa aactagaaac cggaagaaca
    27001 caccagatcc gtgtgcatat gaagtatatc ggttatccgt tagctggaga tccagtatac
    27061 ggaccgaaga aaactttgaa aggcaatggt cagtttctcc atgcgcaaat gttaggtttt
    27121 acgcacccga ctacaaacga aaagatgatc tttgaagcac ctcttccaga agtttttgaa
    27181 aaaactttag aaaaattaag aaaaaacatt gcattttaat ttagataacg gtatagtaat
    27241 taacaacgaa agcataaaac ttaatagaat tcctttaagt ttagtcccgt gaggctaaga
    27301 agggtgggag caaggatacg tatggtgcgc cataagtaga ttgctcattg acatagtata
    27361 gctatcagtg tatcagtata cgctcagtct aaacattcct cctgtcctct tagggcagga
    27421 gtttttttat gcaaaaaagt agggggaatg aaaatgcaag caaaagaagt agtagatcag
    27481 gtaaccatga agcgagcatt gactcgaatt acttatgaga ttatcgagag aaaccatagt
    27541 atccaagata tcgtattggt aggtatcaaa acacgaggaa tctacattgc atcaagaatt
    27601 gccgaacgcc tgaagcagct agaagatatc gatattcctg taggagaact ggatatcact
    27661 ttataccgag atgacaaaaa agaaaatcca gaagaaccgg aacttcattc ttctgacatc
    27721 ccagtttcct tagaaggaaa agaagttatt ttgattgatg atgttttgta tacaggacga
    27781 acgattcgag cagcaatgga cgcagtaatg gattttggca gaccaagaaa aatttcatta
    27841 gcagtcctgg tcgatcgtgg gcatcgagaa ttgccaatcc gtgcagatta tgttgggaag
    27901 aacattccaa catctcgggc ggaagagatt ttagtagaga tgcaagagct tgatggacaa
    27961 gatcgcatca tgattttgaa agaggaagat tagaggagag agaaacatgt cggagaaaaa
    28021 aggatttcat aatgatgacg tggtgttaga tatccacgat cgcccaaccc caggacattg
    28081 ggtaggatta agtttacaac acttgttcac gatgttcgga gcaaccgtgt tagtccccat
    28141 cttagtaggt atcgatccag gtattgcgct agtcagttca ggactaggta cattggtgta
    28201 cttatttact actaaaggaa agatacctgc gtacttagga agcagcttcg ctttcattgc
    28261 agcgatgcag cttttgatga aaacagatgg ttatccagcg attgcacaag gagcagtcac
    28321 aactggatta gtctatttga ttgttgcgct gatcatcaaa aagatcggtt cagcatggct
    28381 ggataaaatc ttgcctccaa tcgtcgtcgg tccagtcatc atggttatcg ggttgggact
    28441 tgcggcaaat gcagcaacaa atgctatgta taatcagggc acgtatgatt tcaagtatat
    28501 tgctgtagcg ctgatcacat taggattaac aattttcttc aacatgtggc taagaggttt
    28561 tctcggattg attccgattt tactaggtat tattagcgga tacatcgtag ccttgctgtt
    28621 tggaatcgtg gatactaagc caatcgtaga cgctgcctgg tttgctgttc ctaactttga
    28681 gattcccttt gtacaatatg aaccaaagct atatcttgga gcaatcacaa caatggcacc
    28741 catcgctttt gtgacgatga cagagcatat cggccatctt atggtattga ataaactaac
    28801 taaacgtaat ttctttgaag aaccgggtct tcataagacg ttgacaggtg atggattagc
    28861 acaaatcgtg gcagcctttg tcggaggtcc tcctgtaaca agttacggtg aaaacattgg
    28921 tgttctggct atcacacgtg tccatagcgt gtttgtgatc ggtggagctg cagtatttgc
    28981 agtcatttta ggattcgtag gaaaattaag cgctttgatt ttaagtatcc ctggaccagt
    29041 catctcagga atcagtttta tcttatttgg agtaattgcc gcaagtggaa tgaagatttt
    29101 aatcgagaac aaaatcgatt ttgataaaaa gaaaaatctg ttgattgctt cagttatctt
    29161 agtagtagga attggcggac tcgtctttga agtaggcaca tttacattgt cggcaatggc
    29221 attagcaact gtacttggca tcattttaaa tcttatctta ccagaaactt cacgaagcga
    29281 agaaaactaa ggaggaaaca ttatgatcat caaatcagaa cgtatcagct taaagcattt
    29341 attgaccgtt gaggcactta ctgatcaaga agttatgggc ttgatccgca gagggcagga
    29401 attcaagcaa ggtgctaatt ggactccgca aaagcgccag tactttgcta caaacctctt
    29461 tttcgaaaat agtactcgca cacataaaag cttcgatgta gcggagaaaa aactagggct
    29521 agaagtgatt gaatttgaag caagcacaag ctctgtccaa aaaggggaaa cactatacga
    29581 tacagtactg acgatgtcag cattaggcgt agacgtagcg gttatccgtc atggtgatga
    29641 gaactattat gacgaactga tacaaagtaa aacaatccag tgttcgatta tcaatggagg
    29701 agacggaagc ggacagcatc caacccaatg cttgcttgat ttgatgacca tttatgaaga
    29761 atttggtcat tttgaaggtc tgaaagttgc catcgtaggt gatatcactc actctcgcgt
    29821 agctaaatcc aatatgcaga tgttaaaacg tctaggcgca caagtattct tctcaggtcc
    29881 tagagaatgg tacgacgatg aattcgaagt gtacggacat tatatgccat tggatgaact
    29941 actagatcaa gttgatgtca tgatgatgct tcgggtgcaa cacgaacgcc atgatggaaa
    30001 agaaagtttc tcaaaagaag gctaccatca ggaacatggg ttaacagttg agcgtgcgaa
    30061 gaagttgcaa aaacatgcaa tcattatgca cccagcacca gttaaccgtg atgtagaatt
    30121 agctgattca ttagtggaag gattacagtc gcggatcgtt cagcaaatga gcaatggagt
    30181 attcgtcaga atggcaatct tagaagcagt gctaaatgga aaagcgtaat tataggagga
    30241 tgaaaaaatg aaaacactca ttaaaaacgg tcagatcaat acacgaaaaa atatgacgac
    30301 accagcagaa atttggatag aagatgggcg aatcaaagca atcggtacag gattctcaga
    30361 agccgaattt gatgaagtgt ttgatgcaaa gggacaattg atcacacctg gtttagtgga
    30421 cgtccatgtt catttgagag agccaggatt tacatacaag gagacgatcg aagctgggac
    30481 tcgctcagct gctcgtggcg gtttcactac tgtatgcgcg atgccgaatt taaatcctgt
    30541 acctgatacg gcagaaaagc tgagacaagt atatgacatc attagaaaag atgcagttgt
    30601 gaaggtattg cagtatgcgc caatcacaga aaatctgcga agtgagaaat tagttgatca
    30661 agaagcactg attgaagaag gtgcttttgc tttcacaaat gacggtgtag gtgtccaaac
    30721 agctgggacg atgtatctgg ctatgaaaga agctgcaaaa aacaataaag ccctagtagc
    30781 ccatacagaa gatgaatctt tattatttgg cggcgttatg cacgctggca agaaggcaga
    30841 agaacttggt cttcccggga tcttaagcgt aaccgagtct tctcaaattg ctagagatct
    30901 gttgctagct gaagcaactg gtgttcatta ccatgtttgc catgtttcta ctaaagaaag
    30961 tgtccgtgta atcagagacg caaaaaaagc aggaatccac gtaacagcag aagtttcacc
    31021 ccatcatctt attttgatcg acgaagatat tcccgaagat ttcggctttt ggaaaatgaa
    31081 cccgccgcta agaggacggg aggacagaga agctttgatc gaaggcttgc ttgacggtac
    31141 gatcgattgc atcgctacag accatgcccc ccatggatta gaagaaaaaa gccaaagttt
    31201 catgaaatca ccatttggta tcgtaggaag cgaaacagcg tttcaactga tctatactca
    31261 ttttgtagaa actggtcgat tcacgttgga acaagtgatc aattggttgg cagttaaacc
    31321 ggctgagatt tttggtttga atgcaggaac gctaacggtt ggtgcccccg ctgatgtagc
    31381 agtttttgat attacacaaa cttgcacgat cgataaagaa gactttttat caaaagggga
    31441 gaatacacct tttatcggct ggaaagtaaa aggcgaaaca caaatgactt ttgtcaacgg
    31501 aaaacttgtg tggcagaagg gagaataaaa gtatggaacg tttgcttata ttagaagatg
    31561 gaacagtttt cgaaggaaag gcttttggtg cagatgctag tgtcttcgga gaaatcgttt
    31621 ttacaacaag tatgactggt tatcaagaaa ctatcactga tcaaagtttt aacggtcaaa
    31681 tcattacatt tacctatcct atggtgggta attatggggt aaatagagat gattatgaat
    31741 cgatttctcc aacatgcaaa ggagtagtag taaaagaaca tgcacgtgta gcttcaaact
    31801 ggcgaaacca aatgacgcta gattcatttt tgaaacaaaa aggaattcct ggaatatcag
    31861 gtatcgatac gcgcgccctt actcgcaaaa tccgtgaagt cggaacgatg aaagctagta
    31921 tagttgatgc tggagattcc ttcgaacatg catttgatca gctgaaagca accgttctag
    31981 caaccaatca agtgcagcaa gtctcaacta acaaacctta tccaagtcca ggaacaggtc
    32041 ggaaagtcat tgtcattgac ttcggcttga aacatagtat tttacgagaa ttgagtaaaa
    32101 gaaactgtaa cctcacagta cttccctata acacagatgc cgagacgatt ttagcgctat
    32161 caccagacgg agtcatgctg acgaatggac ctggtgatcc aaaagatgtg ccagaagcaa
    32221 tcgaaatgat caaaaaaatc caaggaaaag taccgatttt tggtatttgt ctcggtcacc
    32281 agttatttgc tttagccaac ggtgcagata cctataaaat gaaattcgga catcgaggat
    32341 taaatcaccc ggtacgagaa gtagcgaccg gaagaattga ttttacttct caaaatcatg
    32401 gctatgcagt cgatgaagcc acgattgatc cagaaaaact catcgtaact catgtagaag
    32461 taaatgacgg aacagtagaa ggagtcaaac acagagatta tcccgctttc agtgtgcagt
    32521 ttcatccaga tgctgcccct ggtccgcatg atgcactaca tttatttgat gaatttatgg
    32581 aaatgatgga cgcaggaaag gagcagtaaa cagatgccga aacgtacaga tatcaagaaa
    32641 attatggtga ttggatctgg tccgatcatc attggtcaag ccgcagaatt tgactacgca
    32701 ggaacacaag cctgccttgc cttaaaagaa gaaggatatg aagtggtttt agtaaattct
    32761 aatcctgcga cgatcatgac ggataaagaa gtggcagatc aagtatatat cgaaccgatc
    32821 acacttgaat ttgtatcaag gattcttcga aaagagcgtc cagatgctct ccttccaact
    32881 ttaggcggac agacaggcct caatatggca atggagctgg cagcatcagg tattttagaa
    32941 gaattgaaca tagaacttct aggaacaaag ctttctgcga tcgatcaagc agaagatcgt
    33001 gatctattca aacagttgat gaaagaattg aatcagccaa tccccgagtc tgaaatcgtc
    33061 aatacagtag aagaagctgt agattttgca aataaaatcg gctatcctgt catcgttcgt
    33121 ccagccttta ctttaggcgg aactggtgga ggtatgtgtg aaaatgaaga agaattacgt
    33181 atcgtatcag aaaatggatt aagtctttcc cccgctaccc aatgtttgat tgaacgaagt
    33241 attgcaggtt ttaaagaaat tgaatatgaa gtgatgagag attcggctga taatgcaatc
    33301 gttgtctgca atatggaaaa ttttgacccg gtgggtatcc atacaggtga ttctatcgta
    33361 tttgccccta gtcaaacatt gtctgaccat gaatatcaaa tgcttcgtga tgcttctctt
    33421 tcgattattc gagcacttaa gatcgaggga ggctgtaatg tccaactcgc tcttgatcca
    33481 catagtttta attattatgt gattgaagtc aatccgcgtg tttctcgttc atctgcgtta
    33541 gcaagtaagg cgactggcta tccgatcgct aagctagctg ctaaaatagc gcttggatta
    33601 acattagacg aaatgaaaaa tcctgttaca ggaacgactt ttgcagaatt tgaacctgca
    33661 ttagattatg ttgtagctaa aattcctcgc tggccgtttg ataagtttga aaacggagaa
    33721 cggcttttag gcacgcagat gaaagcaact ggtgaagtga tggctatcgg tcggaatatc
    33781 gaagagtctt tattgaaagc tgtgcgttct ttagaaatcg gtacacacca tatcgaactt
    33841 cctgagttgg cagacatcac ggacgatgac ctaatccaaa aagtggttcg agcacaagat
    33901 gatcgcctgt tttatgtagc tgaagctatt agaagagggt acaccataga agaattagcg
    33961 gatttgacaa aaatcgattt attcttttta gataaactgt tgcatatcgt agaaatcgaa
    34021 cagcagttga aacagcagcc gttggatgac aagatattga aaacagcgaa acaaaacggt
    34081 tttacagaca aaaaagtggc ggagctgtgg gaaataaacg aagaagccgt tcgccagatg
    34141 agaaaagaaa atgatttgat tcctgtatat aagatggttg acacttgtgc agcggaattc
    34201 gaatcaaaaa caccttattt ttatagttcc tatgaatatg aaaatgaaag ttcacgatca
    34261 gaaaagccct caattctagt actgggatct ggtcctatcc gtatcggaca aggggttgaa
    34321 tttgactatg cgactgtgca ttctgtcaaa gcaatccagc aggcaggata cgaagcaatc
    34381 atcatgaaca gcaatcctga aacagtttcg actgactttt ctatttcgga taaattatat
    34441 tttgagccat tgaccttcga agatgtgatg aatgtgatcg agctagaaca gccgatagga
    34501 gtaatcgttc aatttggtgg tcagacagca atcaacttag ccgaaccttt gactgctgct
    34561 ggtgtaaaaa tcttagggac aacgatcgaa gacttggatc gtgcggaaaa tcgtgattta
    34621 ttcgagcaag cgctgaaaga gttggatatt cctcaacctt taggggatac tgctacaagt
    34681 aaagaagaag ccgtggttat cgcttcgaga attggttatc cagttcttgt tcgtccaagc
    34741 tatgtattag gcggacgtgc gatggaaatc gttgaaaacc agcgagattt agaagattac
    34801 atggaacatg ctgtgaaagc atctccagaa catccagtac tagtggaccg ttacttgctt
    34861 ggtggtgaat gcgaagtgga tgcgatttgt gacggtgaaa cagtactcat cccagggatc
    34921 atggagcata ttgagcgtgc tggtgtccat tcaggggatt caatggctgt gtatccacct
    34981 caaacattat ctgaatcgat caagaaaacg atcgaagatt acacgatccg cttagcatta
    35041 ggtttgaatt gtgtcgggat gatgaatatc cagtttgtca tccatgaaaa tgaggtttat
    35101 gtgatcgaag tcaatcctcg agcaagtcgg actgttccat tcttaagtaa ggtgacagga
    35161 attccaatgg cacaaattgc gacgaaagct attttgggag agaaactaaa gacacttggt
    35221 tatgaaaacg gactttatcc agaaacaaaa gaagttcatg ttaaagcacc tgtcttttct
    35281 tttacgaaat tgcagaaagt tgatacttac ttagggcctg aaatgaaatc gactggagaa
    35341 gtaatgggat ctgacagaca attagacaaa gctttgtaca aggcattcga agcttctgga
    35401 ttacacttgc ctgaatatgg agccgtatta tttacgattg cggacgaaac aaaagaagaa
    35461 gcactagaat tagctaaacg ttttacagaa attggctata gtctgatagg aacgaaacat
    35521 acagctgctt atttcgaaaa aaatggtctg gtcgttgaaa cagtagcgaa aatttctgag
    35581 gaagcagcag aaaaaaatgt agtcgactta ataagagatg gtaaaacaca agtggttgtc
    35641 aatacgattg ataaagatcg gggaaatgct tctaaagacg gtttcatcat ccgaagagaa
    35701 gcagttgaac atggtattcc attgtttact tctttagaca cagcagatgc gatcgttcga
    35761 gtgatggaat cccgtgcttt ttcaactaac cctatttaac ggcgagcaca tgttaagaca
    35821 aaaagacgaa aaaccgttgc aggaggggac agatgaaaca agaaatgatg gaaatcgttc
    35881 agcaaaaaca gctggcacca aagatttttg aaatgacatt gaaaggtgat ctagtaaaag
    35941 aaatgcagca accaggacag ttcttgcata ttctcgtgcc gcgtgcagac ttgctattac
    36001 gccgaccgat tagcttgaat catatcgacc acgaaacaaa tacttgccgt atcatttatc
    36061 gggtggaagg tgaggggact agagtcttct ctactctttc tgccggagac catttagatg
    36121 tcatgggtcc tttaggcaat ggttttgatt tgtccaatct tcatacagga gacgaagtat
    36181 ttataatcgg tggaggaatc ggtgtccctc ctctatatga gttatcaaaa cagttagtcg
    36241 aaaaaggaat taagccaacg cattttttag gttttgccac acatgaagtc gtttattatg
    36301 aaaaagaatt taatcgtcta ggagaaacaa gaatcgctac tgacgatgga acatacggta
    36361 tgcacggaaa tgtcggtaat ttattgttag gaacgaagac ggaacctaca gctgtttttg
    36421 catgtggttc aaacgggtta ttgaagacag tggaacagct gttttcttcg catcaaaatg
    36481 tccagttatc attagaatcc cgcatggcat gtgggatagg ggcttgctat gcttgcgttt
    36541 gtcatacgcc ggaagatgaa acaggaacaa aaagtgtgaa agtttgtgac gaaggaccga
    36601 tttttaaaat aggagaggta attatatgaa tgagaatcct ttagcggtca aacttcctgg
    36661 cctagaatta aaaaatccta tcatgccggc aagcggctgt ttcggttttg gcaaagaata
    36721 cggaaaatac tatgatttga atcagcttgg atcgatcatg gtcaaggcaa cgactccaaa
    36781 tgcccgcttt ggcaatccca cgccaagagt ggcggaaaca ccatctggca tgttgaatgc
    36841 aattggtttg caaaatcctg gtctagacgt tgttatgcat aaacttcttc cagaattaga
    36901 ggagtacaat gagttaccca tcatcgcaaa tgttgctggt gcttgtgaag aagactatgt
    36961 agaagtttgt agcaaaattg gcgaagcacc aaatgtgaaa gcaatcgagc tgaacatttc
    37021 ttgcccaaat gtcaaacatg gcggaattgc ttttggaaca gattctgatg ttgcttttca
    37081 attaacacaa gcagtaaaga aagtttcttc tgttcctgtc tatgtcaaat tgtccccaaa
    37141 tgtaacagac atcgttccaa tcgctcaggc aatcgaagct ggaggggcag atgggtttac
    37201 gatgatcaat actcttcttg gtatgcggat tgaccttaag acaagaaaac cgattttagc
    37261 caatcaaaca ggtggactct ccggaccagc aatcaagcct gtcgctattc gtttgattca
    37321 ccaagtagca agtatatcca gccttccaat cattgggatg ggcggcgtgc agacagtgga
    37381 tgatgttctt gagatgttta tggctggagc aagtgctgta gctataggga ccgcgaattt
    37441 caccgatccg tatatctgtc ctaaactaat taaagagctc cctgtgcgaa tgagcgaact
    37501 aggcatcgaa tcgctggaaa gattaatcaa agaagtgaga gaggaacgtg aacgatgaat
    37561 caactagacc aacgaccaat tattgcatta gatttttcaa ctaggcaaga ggtagaagac
    37621 tttcttcgat ttttcccgaa agaagaaaaa ttgtttgtga aaatcggaat ggaattgttc
    37681 taccaagaag gacctgaaat cgtacgttat ctaaaagagg ctggtcataa tgtattttta
    37741 gatctgaaac ttcacgatat tccgaatacg gtcgaaaaag caatgcgggg attggcaaag
    37801 cttggtgttg atctcacctg tgttcatgcc gctggcggta ttcgtatgat ggaggcggca
    37861 atgcgaggat tagaagaggg gactccagaa ggtggaaaac gtccgctact tctagctatc
    37921 actcagctta cttctacaag tgaagaagaa atgcatgccg atcaactaat cgaagtttct
    37981 ttagaaaaaa gtgtgatcca ttatgctagc tgtgccaaaa aagcaggatt agatggtgtg
    38041 gtttcttctg cttgggaagt agaagcgatc aaagaaatgg caggagacga atttgtttgc
    38101 ttaactccgg gtataagacc agaaggaaca gtcgcaggag atcagacaag agtagtcaca
    38161 ccttctcagg caaaaaagat cggctcaact tttatcgtta taggtcgacc aatcactcag
    38221 tcaactaatc cttataaagc atatcaaacg attcaaacag aatggagcta acctaaaatg
    38281 aatgttgaac aaagtatcgc aaaagactta ttagaaattg aagcagtatt tttaaatcca
    38341 agtaatcctt ttacatgggc aagtggaatc aaaagcccaa tttactgtga taaccgaatc
    38401 acgatgagct atccaaaagt ccgaaaagag atcgcaaaag gattagcaag caaaatcaaa
    38461 gaagcttacc ctgaagtaca agtgattgct ggaactgcaa cagcaggaat ccctcatgcc
    38521 gcatgggtag cggaaatact ggatttgcca atggtctata ttcgaagtaa ggcgaaagat
    38581 catgggaaag gtaaccaaat cgaaggacgt atatttgaag gtcagaagat ggttgtgatc
    38641 gaggatttga tttctacagg cggaagtgtc ttggaagcag ctgaagcagc aaaaagagaa
    38701 ggtgcggata tccttggtgt tgcggctatc tttacgtatg agttgccaaa aggaaaagca
    38761 aatttcgaaa aagccgaaat ccctcttatg acgttgacca attattctgt attgattgaa
    38821 gcagctttag aagatcgtta tatcgatgag caagaattaa ctctgctaaa agaatggaaa
    38881 aaagatcctg aaaattggca gatttaagcg cgcttgatga agatagagaa atccgatttg
    38941 tgtctccaga atcatcaatt ggaatagcaa aaatccaaaa actgattgcg agaagcatct
    39001 tttcatcagg tatgaaaaga aacttctatc gtgatcagtt tttgattttt ttaaaattca
    39061 tttttctaag actggttagc agaaagaaga gaataatc
//
LOCUS       DAHHPA010000014        39024 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.015, whole genome shotgun sequence.
ACCESSION   DAHHPA010000014 DAHHPA010000000
VERSION     DAHHPA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.015"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(366..1073)
                     /locus_tag="NBH55_000679"
     CDS             complement(366..1073)
                     /locus_tag="NBH55_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508687.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1138..1974)
                     /locus_tag="NBH55_000680"
     CDS             complement(1138..1974)
                     /locus_tag="NBH55_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD1508688.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1992..3401)
                     /locus_tag="NBH55_000681"
     CDS             complement(1992..3401)
                     /locus_tag="NBH55_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD1508689.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3415..5238)
                     /locus_tag="NBH55_000682"
     CDS             complement(3415..5238)
                     /locus_tag="NBH55_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1508690.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6081..6407)
                     /locus_tag="NBH55_000683"
     CDS             complement(6081..6407)
                     /locus_tag="NBH55_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508691.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6453..6815)
                     /locus_tag="NBH55_000684"
     CDS             complement(6453..6815)
                     /locus_tag="NBH55_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCD1508692.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6852..7067)
                     /locus_tag="NBH55_000685"
     CDS             complement(6852..7067)
                     /locus_tag="NBH55_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1508693.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7194..7529
                     /locus_tag="NBH55_000686"
     CDS             7194..7529
                     /locus_tag="NBH55_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1508694.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7605..9602)
                     /locus_tag="NBH55_000687"
     CDS             complement(7605..9602)
                     /locus_tag="NBH55_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508695.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9700..9975)
                     /locus_tag="NBH55_000688"
     CDS             complement(9700..9975)
                     /locus_tag="NBH55_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508696.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10207..10941
                     /locus_tag="NBH55_000689"
     CDS             10207..10941
                     /locus_tag="NBH55_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1508697.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10980..12122
                     /locus_tag="NBH55_000690"
     CDS             10980..12122
                     /locus_tag="NBH55_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1508698.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12217..13608)
                     /gene="sufB"
                     /locus_tag="NBH55_000691"
     CDS             complement(12217..13608)
                     /gene="sufB"
                     /locus_tag="NBH55_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCD1508699.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13613..14083)
                     /locus_tag="NBH55_000692"
     CDS             complement(13613..14083)
                     /locus_tag="NBH55_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCD1508700.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14070..15305)
                     /locus_tag="NBH55_000693"
     CDS             complement(14070..15305)
                     /locus_tag="NBH55_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1508701.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15302..16588)
                     /gene="sufD"
                     /locus_tag="NBH55_000694"
     CDS             complement(15302..16588)
                     /gene="sufD"
                     /locus_tag="NBH55_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCD1508702.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16601..17374)
                     /gene="sufC"
                     /locus_tag="NBH55_000695"
     CDS             complement(16601..17374)
                     /gene="sufC"
                     /locus_tag="NBH55_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCD1508703.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17571..18419)
                     /locus_tag="NBH55_000696"
     CDS             complement(17571..18419)
                     /locus_tag="NBH55_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1508704.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18443..19129)
                     /locus_tag="NBH55_000697"
     CDS             complement(18443..19129)
                     /locus_tag="NBH55_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508705.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19122..20156)
                     /locus_tag="NBH55_000698"
     CDS             complement(19122..20156)
                     /locus_tag="NBH55_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1508706.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20219..20475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20551..20889)
                     /locus_tag="NBH55_000699"
     CDS             complement(20551..20889)
                     /locus_tag="NBH55_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCD1508707.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20891..21247)
                     /locus_tag="NBH55_000700"
     CDS             complement(20891..21247)
                     /locus_tag="NBH55_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCD1508708.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21253..22440)
                     /locus_tag="NBH55_000701"
     CDS             complement(21253..22440)
                     /locus_tag="NBH55_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCD1508709.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22631..23476)
                     /locus_tag="NBH55_000702"
     CDS             complement(22631..23476)
                     /locus_tag="NBH55_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1508710.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23710..24675
                     /locus_tag="NBH55_000703"
     CDS             23710..24675
                     /locus_tag="NBH55_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCD1508711.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25052..26095
                     /locus_tag="NBH55_000704"
     CDS             25052..26095
                     /locus_tag="NBH55_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCD1508712.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26157..26621)
                     /locus_tag="NBH55_000705"
     CDS             complement(26157..26621)
                     /locus_tag="NBH55_000705"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCD1508713.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26867..27814
                     /locus_tag="NBH55_000706"
     CDS             26867..27814
                     /locus_tag="NBH55_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1508714.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28025..30097)
                     /locus_tag="NBH55_000707"
                     /pseudo
     CDS             complement(28025..30097)
                     /locus_tag="NBH55_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            complement(30030..30407)
                     /locus_tag="NBH55_000708"
     CDS             complement(30030..30407)
                     /locus_tag="NBH55_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCD1508715.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30536..31114)
                     /locus_tag="NBH55_000709"
     CDS             complement(30536..31114)
                     /locus_tag="NBH55_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1508716.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31246..32538
                     /locus_tag="NBH55_000710"
     CDS             31246..32538
                     /locus_tag="NBH55_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCD1508717.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32598..33716)
                     /locus_tag="NBH55_000711"
     CDS             complement(32598..33716)
                     /locus_tag="NBH55_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCD1508718.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33730..35151)
                     /gene="arcD"
                     /locus_tag="NBH55_000712"
     CDS             complement(33730..35151)
                     /gene="arcD"
                     /locus_tag="NBH55_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCD1508719.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35397..36518)
                     /gene="mnmA"
                     /locus_tag="NBH55_000713"
     CDS             complement(35397..36518)
                     /gene="mnmA"
                     /locus_tag="NBH55_000713"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCD1508720.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            36922..37521
                     /locus_tag="NBH55_000714"
     CDS             36922..37521
                     /locus_tag="NBH55_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCD1508721.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37659..38945)
                     /gene="efmA"
                     /locus_tag="NBH55_000715"
     CDS             complement(37659..38945)
                     /gene="efmA"
                     /locus_tag="NBH55_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCD1508722.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
BASE COUNT        11252 a         8213 c         6828 g        12731 t
ORIGIN      
        1 acaattacat atgatttttt cataaaaaaa cagtactgtc tagtagtata aaatatacaa
       61 atttcttaaa aattttttca ctaaaatcac tacctgaaac agtctaaaac acttatatat
      121 caacgtttaa tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc
      181 aaatccgaaa aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg
      241 gtcatataac aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata
      301 acttttttaa atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa
      361 tctttttacc cattttcaag taaccgaagt ggaatgtctt taccaacttg ctctgtcact
      421 gcattactga tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa
      481 atctctgtat cagtatttat cgtgattcct ttatcttcat ttacaagctt ccaagtaggt
      541 ggtatagctg tgtatgtttc gatgaatgaa tcaattcttt catctatcgt cttcccagta
      601 tctacttctg tctcattact cttagatgaa tcgattcttt catccatcgt ctgctccgta
      661 actgcttctg tctcattatt ctcaccggaa actctcttca cacccaaact tatatattgt
      721 ccatctttag tgtgcatagt taaaattccc acgcttttac taataaattc actataacca
      781 gttctttcaa tagagacttc aatgtatttc acactatcat actcaaaaga aaaatagaca
      841 tgtaaaagca ttttgttgtt tacatctaca taataatcct ttgactctac ttgagacctt
      901 cctatacgga atacagcttt actgtctgat atctctatac cagcatcctt attattaatc
      961 tgccaatctc caagtagctc ttctggcaag ctagtagttt cttgtgaaca ctttaccact
     1021 tcactggtag aggtcgaaac attcacctcc gcactgttag aagaattttc caaggaatca
     1081 ggagccttag cacaaccaac aagtctttat atattgtgca gccgatattt tcttcattca
     1141 gtacaccctt gaaatatgca gtgttagata aagcagttcg tcctgatttg ctgaggcacc
     1201 catctcttct tgaacgtact ccttgatttt caaggccgcc cgatactctt tcggaaaacg
     1261 ctgcttcact gaagccagca aaaaatcatc cttctccggc aactcttcct tcttaatcat
     1321 tcgggcaata aaaaactgta aatgcgtcac aaatcttaag taaagcatcg aatctttatc
     1381 aacctttttt tcgggaaaca gatatcctag catagccaag atatccttta gtgctcttac
     1441 cgagagcatg gtttcttcca tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc
     1501 aatgtttcca gcctcctctt ctggcaactg aacattcatt tttttgttga tcaattggat
     1561 agcatagcag ccgatctcaa attcttttgg gtaatacttt tgaatttcaa tcagcaggcg
     1621 attttgaaaa acaatgcctt tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa
     1681 ggtaaaaaat agacttttat tcagcttaaa gtcaaacttg gcgttggcat aatcgataat
     1741 tcgcttgacg caaataatta gatcatccgg agcagcctca aacagtttaa tgtaatcctt
     1801 ttttagagca tcagaaagtg aatctaattt aaatactttt tggattttgt cagccggaac
     1861 caaatcgcct ttctttagtt gaaatccaat ccctttcccc atcaaaatta cttctttgcc
     1921 ttgcttatct aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc tcattagatt
     1981 caccgttctc tctaagctaa gccatttctt gcgattactt tttgatacca aaagaagctt
     2041 tcttttttta ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac atatataaaa
     2101 ccataacgct tattgaagcc ttgatgggta ctaacaacat caatcgctga ccaaggtaaa
     2161 taaccaaaaa catctacacc atctgttaaa gctgcttgaa tagccagaat atgtttggct
     2221 agataatcaa ttcgatcctg gtcattaatt cgtctatctt cctcaactat gtcgggtgcc
     2281 ccatagccat tttcagtaat taaaataggt aaatcatagc gatcgtacaa tttcctcaat
     2341 gttaatcgta gtccagtagg gtcaactacc cagccatatg gcgttttatc tacataagga
     2401 ttttcagctg cccggtagac accttcttct cccaacatga tctgttgatc ccccgctcta
     2461 gcggaaacat ctgatccgtc atttttgctg gcagcgatgg tggcagttga ataataatta
     2521 atcgcaataa aatcgggttt accggaggat aaaattgcct gatcctctgc ctcaatttta
     2581 ggatataatc cacgatcatt caaatagttt tcaaacaaat aattgtattt tccccgaact
     2641 gctacatcta agaaagacca gcctctcaat gtttcccaat tatgggctgc aattgcatct
     2701 tctggactgg aggtagcttg atacatagcc gttaaattga ttgctggacc gatttttcct
     2761 ttaagattca actgatgata caagtgaatt actctagctt gagctaataa aacatggtga
     2821 ttaatttgaa agagttcttt ttttgaaggc agctctccac ttggtggcaa accaatcgct
     2881 ccaggatgaa ggatcatcgt gttttgctca ttaattgtca gccaatagtt caccttgtca
     2941 ccatactcag taagcaatac ctgtacatag tccacaaaat aatcgatagt tgctcgattt
     3001 aaccaaccgc cctgctcctc tagaaaataa ggtaaatcga aatggtacat ggtgacaatc
     3061 ggttcgattc cctgattcag gcattcatca attacttgat gataaaaaga aattcccttt
     3121 tgattggttt tattattttc aatatccata attcgcgtcc aagcgatgga aaagcgaaaa
     3181 gcttttaagc ctaattcttt aaacagctgt atatcttctt tatagtgatg gtaaaaatcc
     3241 gaggcaactt caaaattagc taccccttct gtatgctgat acttgtcaat aatagacggt
     3301 tgtttcccat cctcctgata ggcaccctct gcctggtagg cactaatgga ggcaccccat
     3361 aaaaacttct ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct ctccttaact
     3421 aatttgcatc aatggttcgt gttcctttaa gcttccgctt gcttcactaa ttcctgcgta
     3481 attttcactg ttggtcacta ccacaatcac tgtatcatcg tagccagcgt ttctaatggc
     3541 agcgaaatcg gcgttgatta ataattgtcc tgctgacacc tgatctcctg cttttacaaa
     3601 tacttcaaag ggttcccctt ttaaattcac ggtgtcgatc cccacatgga tcaataattc
     3661 aataccttct ttagaacgta gaccaatcgc atgtttagat tctaaaatag taatgacttc
     3721 tccctcaaca ggtgaataaa tgctgttttc ttctggatga atggctacgc cctttcccaa
     3781 tagtccctta gaaaagacag catcgttcac ttcagaaata ggacacacgg ttccttttac
     3841 cggattttca acaatgactg cttcggctat tgccgtttgc tctgattcat cagaagtatc
     3901 gtcaaatcca attagataag caatgataaa ggttgcaaca aaggcgatta cacacgtaat
     3961 aattgcgtga acaatattca ttggattttc tccaataaag gctggtaagg ctgctaagcc
     4021 aggggaaaca aaggcatatc tgactaaacc gctcaagcca gcatacaaac cagcaattgc
     4081 accaccaatc atggaagcaa ctaatggttt tttcagcttc aaggttactc catagagtga
     4141 aggctcagta attcccatta aagcagtgat cccagtggac acagctagct gcttcaactc
     4201 tttgttttta gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa tattagaagc
     4261 cagcattcct ggcccattga tcatttcaga acccgtactg ctcaattgca tagtagcgat
     4321 tggcgtcatt gcccaagctg tacccgtaac caccaaaaat ggctgcaaac cacccatcaa
     4381 aaatggaatt aaccaagctg ctttgccgtt aatccattca gcccccattg ctataagatc
     4441 gttcagcaac gttccaaacg gtccaatgac aaccaacgct aaaggcccca taattaaaaa
     4501 taccagcatc ggcttagtaa agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg
     4561 ctctacatag gacatgatcc aaacagtaaa gataatcggt aaaactgatc ccgcataatc
     4621 tgcagctaat accggaattc ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga
     4681 aatatttgga tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa
     4741 ctttatggct gccccatagg ctaatagtac tggcataaaa taaaaggctg catcagcaat
     4801 agtattcaaa atgatgtaag tttgactact gtctgatagt acaccagtca aagtcaagac
     4861 ggccaaaatg gcttttacca tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt
     4921 ggtagaaatg acactaatca atcttgaaat aatactttct tgattgtctg aagaggggcc
     4981 ctttttaatt gaagttccca cttccttttg aatttccttg aaggctgttt ggacttcatt
     5041 gccaatcacc acttggtatt gtccaccgct atccaccaca ctaattacac caagtgtttt
     5101 ttctaaggct ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca gtctggtggc
     5161 acaatgggtt aagctgacca cattttcttt tcccccaacc tgttgcaata catttcttcc
     5221 tacttctctg taatccatta ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc
     5281 acgccccaat caacattgaa gtgttagttt gtcttggtct tgcctgcata atcagtaaca
     5341 tgccaataac gttattttgc tgtaatccaa taatagcaca ttatgatagc gcaatcaaca
     5401 gggaatttaa ttttttatct tttcgtttga ccgtataggg taatataaca gttagatcac
     5461 atcaactagg gtatatctgc gtatacttac tgctaaaata agtatatcta atattaacta
     5521 taccagtaaa accattccta cataaccagc gggaaattcc caaaatcgtg aaagcatcaa
     5581 aaatgcatat aacggatcac acaacttcaa caattctgct cttgggaaaa attgatgaat
     5641 ttggtgagta aaggatggaa taaacagcat tccaacaata taggcaacaa tagtgatact
     5701 tgtttttttg gtaaaaatca gtaagcaata gccaatttca atcattccga gatataaagg
     5761 tatcaataga atgatttggg ctagtattct taaaaattaa ctagcaaatg tttaaactga
     5821 tttatttcta atctacatgt agtaagtatt tcaattccac caattatcaa taaaaatacc
     5881 aaacatatcc ataataattt tttctttttc atttctttta tattgttata acagtgaata
     5941 acataaaaat tcccgacaat cattgtaata tttttttgtc acctgtatta gtatttaatt
     6001 tataggaaac tataaaaact ccctagtgaa tttacacctt tgggagttcc tgtttggacg
     6061 gtatgcataa tacatttaaa tcaatttaac attaacacct cagcatatgg tatctccaca
     6121 tatatacggt ctttctttgg atagtactca atatttatgt tgatagcact tccattgtct
     6181 aatatagctt tagcccggta tgtgttatct ccattttcat gagtaatcgt cacatcatca
     6241 catgatcgat ttaagttgta ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac
     6301 ttctctaatc cagatgtacc tgtcattttt gaaaaaataa tacctataat agccaagaca
     6361 acaataccta taatgacaaa cttcttgttc atatttactt tattcaactt tttatctcct
     6421 atctccaaaa taatttgttc ttatccctct tatcagtaca ttgacatccc taaaagtaca
     6481 ccaaagattg ctccagctac ccctgctatc atcaaaatcg tacccatttt ctcatctttt
     6541 tctcgatata agtagcccat aataacagca acagcaccaa atacaacagg aataaatgct
     6601 aacgatacta ccatagaaat ccaaccaata atcaaataca gcttccctgt ctcttgtcca
     6661 tgtacgattt gttccttcat ttcattaatt gtctgctcag tacttttctt tactgtgtct
     6721 gttccggttg tctcatctgt ttcttgtagt gctccacaaa actggcaata cttactttca
     6781 tctgttattt ctttgccaca ttttctacaa tacatgtctt ctcctcctaa aatcattcaa
     6841 ttgcttatta gttaactgtt tactttatta aatacatcag aaaacagctc attcattggt
     6901 acacttaata tctcagaaag catcttcatt tgatagggtt taggaatcgt tctccctgtt
     6961 tgccacgaac ttacacattg ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa
     7021 agtccttttt cttttctgta tcggctgaga ttacttactt ttttcatgat tctcccctcc
     7081 tgttttatta tatacaacaa tatgatgtat ttcaactcat aaaatataaa aaacttcaaa
     7141 taccattaca aatacaacaa cttgttgtac tatactgaga tgggggtgta ttaatgaaac
     7201 agaaacaata tgcaaaggtc attagagaaa agcgaaaagc tctcgggtgg acacaaaaag
     7261 aattagctga aaaaatcttc tctacacaac aggcagtagc aagatgggaa aattctgtaa
     7321 cagagcctaa cctcgatagc ttaacagctc tttctagagc cctaggaacc cctgtaagcc
     7381 attttctaga caatgttgtc gtagattatg aggaagagtt tttggctctc tatcgttctc
     7441 ttagcactga agatgcagtt cgaacaattg actatatgaa gctattaaaa aggcaagaaa
     7501 atgaacgcaa tcagctattg aaagattgaa aaataaataa cccaccgaac tttaacggtg
     7561 ggtgtattga gatgaaataa gattaagagc tatggataac ctgtctagtt taatacaatt
     7621 gggtcttttc tcttccttac tctccgtcca tcatctcttg ttacacttgt aaattgatac
     7681 agaggaacta tcccagctcc tgataatatc aattctttta ttgtttcatc tatccctgcc
     7741 cctttcgcta aatatattaa aaaactacta cttgctttca cattgtatag tttcttaata
     7801 tccttagaag ctcgttcaag agtgaatcct tgtttaacaa agtagcctct gttttttatt
     7861 aaagcatttt tcacgctgtt atccccaaca ttacgtaggc gttcacaaac agtgttaata
     7921 agttctttct ctgtcattag atatgtccct tgccacgata agccctgtaa aacttcatgt
     7981 gcttcatcta aaatcaattg tttttccttg ctgagttctg atcgggaatt ttgaggaaat
     8041 actcttttag cctctttttc gttaaaacat atcttaaccc attcataaga aaaatattga
     8101 tatgtaaaat gaattttttc cagaagtgga aggatatctt cattcaattt ttgaatcaca
     8161 gaagttgcat taaaaacgtc taatcgatag acattgcttc tcttatgatt aaagtttcct
     8221 aacgctttct cttcctttaa cctatttagg tattcgagca tcttcaaagg aacatcttca
     8281 tcatctaatt tttttatcaa tcctataaaa gtcataagtg atagtaactt attacattta
     8341 tctatgtcat aagttttacc atagtaagaa ctcatttttt tagctaattg ttcccccgac
     8401 aaaatacaag tcacactttc cttactatca tcaaaaaaat taacatcaga aagcaagaca
     8461 tcatagacac gtcgcatttc ctctagataa ttccttattg tcctgaaaag tttgtctttc
     8521 attgattcaa gagtgtcaaa aagtactttt gattcctctt tcaattttcg aaaatgtgta
     8581 ggatacaagt ctaggttctc taatagccaa ttaaatgttt ctacccgact gtctttcttt
     8641 aaaattagca tcacaactcc aataaaattt tcactgatat cacaattact ggaatgacaa
     8701 taaaaaatgg aatgcccagt tttactggta aaaatactag cagagggatt atgatcgttg
     8761 tgaaacagac atcttaagtt tttacttgac ttaagtaatt tattcatatc aatggttaac
     8821 aatctttcat aaatctcatt aaaagacatg tcggaggaat caaacaggca gtctttaaac
     8881 cactgtttca tttcttcttt gtcgtcattt tttatcaact taacaaaatc ggtttcattg
     8941 actagttcat actctctccc tgaatcactt tgacaatttt tagaacatgt catggtaaaa
     9001 tcaatcggaa gctcagtaat atctaacatg ttgtcttcat taattattat cactgcatct
     9061 gacttatatc ctccaaaaaa cattctattc gaagcattta cattgtcatc acacccgggg
     9121 atgttctttt tcaagtattc gtaaacagca accattttac catagtcagt aattgcttct
     9181 tttaatagaa agacaatctt gtatttttct agattttcag agtaccgaat acttttttgt
     9241 atcgcaaacg cagaattatg aactaaattg atagataaaa ccttttctat gcttaagtat
     9301 ctactatcat ctttactcaa atctactacc ttccccttct catctttaac agtgttatcg
     9361 acatccagaa taattactct ggtttccaca aaactctcct tgtcattgcc tttattcttt
     9421 gtcttgcttg gaataaatga ttgtgatgaa tttagtatct ctacaaactc atctcttgta
     9481 acttctctat agttatctaa acacctatct cttacatcag ttttttcttt ttttgtaact
     9541 ggcttctcta ataatttttc ttgatctaca taaattggaa tattaatcat tgatgtttcc
     9601 atttgtgttt cctccttata aaattctgtt aaagactaaa acatacagat aataaaaaca
     9661 aactctttat tactatatgt tttagtctta tttgtttact caacctttat accgttccat
     9721 gaaactatca atatcagatt ttttaaatct aatgaccgta tcaattttta taacaggtaa
     9781 cccgtattga gaaacatatt ttctaatcgt tcctactgat acacctagat attcacttgc
     9841 ctgctgatag tccatatact ctagagtgac tccagaaata atgtcttcta gcataggctt
     9901 tagcactgta attatctctt tcacttgttg ttcaacttgt atagaaacaa tgctatcaat
     9961 gatttgtaga gacatatttc cccctccttt cgtgattgct tgaacaaatt ttctaagaat
    10021 tatcttcgta ttttttgctc aaaataaaag tattttgtta ttaaccgatt aatcaaacaa
    10081 aaaatgatta ccagttcatc gacatacaca taatacctcg ataaaaaagg ggtgtcaaca
    10141 gctttgtaaa atttatttac aacacccctt ttttgaggta tactaatttt aggaggtgtg
    10201 gtcaatatga taaaacgtcc accaataaat tatttagaac gaaaaaagat actcggaaca
    10261 aaaataaaag ctataagaaa gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt
    10321 aacaacggtc aattaattga caaaaaaact atctacgaat gggagaaggg aacctattta
    10381 ccaataccag agcgattatc ccgaattgct gatttgggaa atatgagtat agaggagtta
    10441 gtttgtggca atgtagaaga atatattttg ggaattattc tttatagaga tagcattgta
    10501 ctagatggta taacatttcc agataaaaac ctgttccagc atcttcgtca acagttccca
    10561 ccagtacact ctaatttaga tacatggctg gatagatatt ccaaattaga accagagatg
    10621 caagaattta ttgctaacaa gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc
    10681 aatatcttaa aaatagaaga attattcatc aatgctattg tagaagaatt tgacaataac
    10741 attcttttcc tcactagcag tatagaagaa ctactagaaa gaatggtaga tgaatggcta
    10801 cctattcaat tgaaagacat gtcttatccc gaagaagctg tgagagaaat tactgataat
    10861 ataaacaagc tagaacaaac gatttcatca attgggaaaa aatacactaa gaaaaaaatg
    10921 aagggaggtg ataccatcta gtggtaagta ctccttgtaa actaagagga gatgatttaa
    10981 tggcaaaaat atcaaagtac aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac
    11041 acattgccac cgatccacga actggtgaaa aaataaatac tacgagaaga ggttttagta
    11101 gcaaacaaga agctcaaaaa gattatgacg agtacagaca tcaaatatta tacggtgtta
    11161 aaaagaaagc acctgatatg acatttgaag acctgtataa tgaatggata actcatcagc
    11221 gtaccagtgt taaagcttca acaatcgcaa tctcagtaag atacgcaaat aatcaaatac
    11281 ttccagcatt tggtaagctt aaactatcga atatctctgt cccatattgt caaaaagtgg
    11341 tagatgaatg gcacagtaaa tatgaaagtt acgattatat gagaaaacag actgcacaaa
    11401 ttttacgcta cggggttgca atgcaatata ttgacaataa tcctatggaa aaaacacttc
    11461 ttccacgaaa aaaagagtat gagaaaaatc gtaaatttta ctcaaaagag gagcttaata
    11521 atttactgga tgcttttaaa gattttggta acatgaaaca atacgccttc ttcagattat
    11581 tggcatacac aggtatgaga aagagtgaag tactcgcttt acagtggaaa gacatagaca
    11641 cgtttaataa agaactacat gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta
    11701 taattcaatc tccgaaaaca agagcttcta gacgagttat ctctttagat actgagactc
    11761 tttctatcct caacaactgg aaattacagc aaaaagaaga gtatttaaaa cttggataca
    11821 atactagctc taaagagcaa catgttttta caacagttaa aaacactcta tacattccaa
    11881 acactgttaa tgattggtta agatatattt tgaaaaaata taacttgcct cgtattacac
    11941 ctcatgggtt tagacataca catgctagtt tattattaga agcaggagaa tctgtcaagg
    12001 tagttcaaca acgattaggg catgaaaata gtaaagtgac attagacatc tatgcacata
    12061 ttacaaataa tgcacctaag aaaacaggtc aagactttgc tgatatgatg gctcatcagt
    12121 aaaagagctg gtcaaaatct ggtcatttct agtatacagg caaaacaaaa aagctagaac
    12181 ccttatatat caagggttct agctcctata acaggtttac ccaacactgc cttccatctc
    12241 ataactgatc aatcgattga gttcgactgc atattccatt ggaagttctt tggtgaatgg
    12301 ttcaacaaag cccatgacga tcatttcagt tgcttcagat tcggtcaggc ctcggctcat
    12361 cagatagtag agttgttctt ctgagatttt tgagactttg gcttcgtgtt ctaaagagac
    12421 ttggctgttg tggatctcat tgaatgggat cgtatcggat ttcgaccatt cgtccatgat
    12481 gatcgtgtca cattcgatat gagagatcga acccgcactg tttttggcaa aggttacttg
    12541 tcctcgatag tttacttctc cgccgtcttt tgaaattgat ttagagacga tagagctaga
    12601 tgtatttggt gcattgtgga tcatttttgc acccgtatct tggatttggt ttgctcctgc
    12661 aaaggcgatg gagagcatgg tgcctcgtgc accttttcca tctaagtaaa cacttgggta
    12721 tttcatcgtt gtttttgcac ctaggtttcc gtcgatccat tcaacggttg cgccttcgta
    12781 tgcttttgct cgtttggtta ctaagttgta cacattgtct gaccagtttt ggatcgttgt
    12841 gtaacggcaa tacgcatctt ttcttgtaaa gatttctacg atggcggcat gcaggctgtt
    12901 acttgaataa gtaggtgccg tacaaccttc gacatagtgg atgcttgctc cttcgtcaac
    12961 aatgatcagc gtacgttcga attgccccgt gttttccgca ttgatccgga agtacgtttg
    13021 taatggaaca tcgactttga cgccttttgg cacgtaaata aaggtcccac ctgaccatac
    13081 ggctgaattc agcgctgcta atttgttgtc ggttggcggt acaagttttg aaaagtactc
    13141 tttgaataat tcagggtatt ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa
    13201 cttttcgaat tcatctttca tattatggta cacgacttct gattcatatt gtgcggaagc
    13261 accggctaga taagctcgtt cagcttctgg tataccaatc ttttcaaatg tatctttgat
    13321 tttttctggt acgtcttccc agtcacgagc gggtttgtca cttggttttt ggtaatattt
    13381 gatggcatcg aaatcgatgt ctgaaaggtc ggggccccat gtttgcattg gcattttgtt
    13441 aaatgtttct agagatttca gacggaattc aagcatccat tctggttcgc ctttgattct
    13501 tgacatctca cggatcactt cttctgtcaa gccttctcct gtactgaaaa ccggttcgac
    13561 atcatcgtga aacccgaatt tatattcttc taattctggt acgcccatgt tctcactcct
    13621 cttccttgtg gtaatgatgg aaagctgcgg taccatcgtt ttccattgct tttcctagtg
    13681 ctttccaggc aagtgtcgca catttgatcc gagcgggaaa tttagctaca ccaccaagca
    13741 tagcagcatc gcctaattgg tcgggatctt tgacttcttt tccttggaca agctgtagaa
    13801 agtctacagt taaatgttct gcttcttcta ttgattttcc gatgacggcg tctgtcatca
    13861 tcgaggcact tgccgtactg atggagcagc cactgccgtc aaaggcgata tttttgatga
    13921 ttccgtcttt tacatcgact tgcaattcga tcacatctcc acatgtgggg ttgttcaatt
    13981 cgatcttgct tgtcgagtca gtcaagcttc cacgatgatg cggatgggaa gaatgatcca
    14041 agatcacttg acggtataaa ttgtctaatt tagatagtcc catgttggaa aaactccttt
    14101 gttgccatga ttgcttcaac taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag
    14161 ctcgcgcgag cagtcgctga tacttcaagg tacttcaata gcggttgcgc acaatgatga
    14221 cctgctctga cggctacccc ttccatgtct aaagctgtcg cgacatcatg gggatgtaac
    14281 ccatccaaat tgaatgcaac gactcctgtc cgaaaattcg ggtcttgtgg accataaaca
    14341 gtcaatccat caattgcttg taattttggc aacacataag cggctagttc ttcttcatat
    14401 cgatggatat tgtccatacc gagtttttgc aaataatcga ttgcatggcc taatgcgata
    14461 gcgccggcga tatttggtgt ccctgcttcg aatttccaag gtagttcttt ccatgtactg
    14521 tcgtataatt cgacaaaatc gatcatttcg ccaccaaatt caacaggttc catttcttct
    14581 aaccattttc gtttaccata taaaacacca atacctgttg gtccgcacat tttgtgtccg
    14641 ctgaaagcaa agaaatcaca gtctagtgct tggacatcaa tggccatgtg cggggcagat
    14701 tgagcaccat ctactacgat gaccccgccg ttttcatgga caagttcagc taattcttga
    14761 acaggattga agacacctaa gacattggat acatgagcaa tggagacgat tttggttttg
    14821 tccgtaatct ttttcgctgc atcctccata tccaggaagc cttcttctgt tagttcgata
    14881 tatttcaaga cagcacctgt tcgttttgcc aactgctgcc acgggataac attagaatga
    14941 tgttccatgt aagagagcac tatctcgtct cctgctttga tatagcggtc gccgaaggtt
    15001 ctggctaccc aattcaagct tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct
    15061 tttgcatgga tgaacgcccg tactttttct cttgcttgtt cataatcatg ggtcgcgcgt
    15121 tctgctaacg tatggacacc gcgatgaaca ttggcgttat cgtgttcgta atagtacgct
    15181 aatgcctgga gtacttgttt tggcttttgg gtcgtcgctg cattatccag ataaactaaa
    15241 ggttcatcat tgacgtcttg aaataagata ggaaaatcat tgcgtagctt tttagcatcg
    15301 atcatccatt caacttccct tcgattactt cgactaattc tttccgaact tgttctacag
    15361 ggattgctgt taagacagaa ccgaggaatc ctcgaatcac taagcgctct gcttcttctt
    15421 tatgcaatcc tctactcatc agatagtaca tttcttctgg atcaactcgt cctacactgg
    15481 cagcatgtcc agcagttact tcgttttcat cgatcaaaag aataggattc gcatcaccac
    15541 gtgctttatc agaaagcatc aagacacgac tttcttgttg ggcatctgca ccttttgccc
    15601 ctttcaagat gtggccgata ccattaaagg tcaatgttcc tttttctcgg atcactccgt
    15661 gttgcaaaat atgcccgatc gtatgagggg ctttattggt tactcgtgta tcgattcctt
    15721 ggatttgtct gcccgagctg attgccacga ttttgacttc tgcgtgtgca ccttctcctg
    15781 ctaaatctga gtcaaagtca gcgatcacat gaccgtcatt catgacacca attgcccagt
    15841 cgacagaagc atctcgtaaa atgtgcccac ggcgattcat ataagaagta atattttctc
    15901 ctaattgatc tacggctgaa tatttgattt ttgaaccagc tttagcgatg acttccacga
    15961 tgatattacc tgatgatttt gctacttgtt ctttggttgt ctggaaacgt tccagataag
    16021 aaaattcact atgttcatct gccacgatca acacatgctt gaagaagtgc tcatcggatg
    16081 cgccatcttg aatgaataag gactcgagtg gttcatcgat cacgacattt ttaggcacat
    16141 acaggaaaat accgctgttc ataaaagcta catggagtgc agtcaattgg tcttcgttca
    16201 tgtttactgc ttttttcata taatattctt ggactagttc tggatattcg atcattgcag
    16261 tgaacaagtc ggtaaaaatg actccttttt ctgctaattc cacaggcagt tgttcaaaaa
    16321 tcgtccatga accttgttgg acaagcacag gattgtcttt cattgcatca aatgccggaa
    16381 tcgtgcctgt ttcactgatg gtttgtgttt ctttcacatc aaataacgac cagcgatgga
    16441 atttcacccg atcgatatgc ggcaatggca gttcatctgc tttcgctagt gcgtcttgac
    16501 ggaaggttct catccaatca ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa
    16561 gataatcgag ccagtttttt tctttcattc ttgctccccc ttacgcttct tcttctttat
    16621 agtcgattcc taattcttta ctgataccag cgtagccttc tgcttccaga cgtttagcta
    16681 ggtcagcatt ccctgtcatg actactcggc cttccatcat gatgtgaacg acatcaggtg
    16741 tgatatagtt caataatcgt tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc
    16801 gcatttcatt tactccttta gcaacgactt tcaaagcatc gatatccaga cctgaatcga
    16861 tttcgtctaa aattgcaaat gtaggttcta acatcaacaa ttgcaggatc tcattccgtt
    16921 tcttttctcc gccagaaaaa ccttcgttca gataacgttc agccatttct tctggcatat
    16981 ttagtaaagc cattttttca tctaactttt tcaaaaatgc cataacagaa attttgtcgt
    17041 tttcatcacg tttcgcattg atcgctgcac gcatgaattc cgcattcgtg atacctggta
    17101 tctcacttgg atattgcata gctaaaaata agccaagtcg cgcacgttca tctacttcca
    17161 agtctaaaac atttttcccg tcaaacaaaa tttcgccttc tgttacttca tagtttggat
    17221 tgcccatgat agcggcagat aaggtagatt ttcctgtgcc gttaggtccc atgatggcat
    17281 ggatttctcc ggttttcatt gttaggttta cgcctttaag gatttctttt tcctcgatcg
    17341 acacatgtag attttttatt tctaagacag acatgcatta tacccccaaa ttctctttcg
    17401 ttctcactca ttttagacta attgagaatg aagcgcaatg accttcctta ctaaaatcaa
    17461 atatctttta tttattatta aacaattttg atttctaaat tagaataggt ataaaaaaga
    17521 tgagaaataa gccattacag acttatttct catctttttt gttccatacc ttattttgca
    17581 tcagccttta ctggtttaac agagccgccc catttgtctg tgatccattc ttggacttct
    17641 ttgcggtgaa gcacttcaat cagttttttg attttgtcac tgtcttcatc acctttacga
    17701 gtcgcaataa tattcacgta aggagagttg tccttttcaa gcaatacggc atcttctaat
    17761 ggattcaacc ctgcgccgta agcgaagttt gcgttgattg caactaggtc accttcatca
    17821 ttttggtacg tagaagctaa aagtgatggg tcgatcgtat ggttgaactt caagttctta
    17881 gggttttctt caatatcatc gaatgttgct gtttccagat ctactccatc tttgactttg
    17941 actaagccag catcttgtag aattgtgatg atgcgtcccc agtctgattc agaatttgaa
    18001 gtaatgattg ttgcaccatc ttttaattcg gaaacatctt tgactttttt agagtacaag
    18061 cccattggtt cgatgtggac agcaccggca ttgacaaagt catatccttt ttctttgatt
    18121 tctttgttca aataaggaat atgctggaag taattggcat caatgtcgcc ttcttccaaa
    18181 gattggttcg gtaatacata gtcatcgaat ttgacgattt caagatcgat tccttcatct
    18241 ttcaataatg gtttcacatg ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact
    18301 ttcaatgtcg tgtcgtctgc tttatttgct gaatctttgc tgtcgctgtt gttaccgcat
    18361 gctgctaatc ctacagtcag caaaagcgct gctgcaaatc caaaaatttt ctttttcata
    18421 tcctacatcc ccctatatcc tattatcttt tgtctgtttt cttagttaag aaatcaccga
    18481 ttccttgaac gataaatacg attagtaaaa taattaaagt cgcaacgaat gtcactgtta
    18541 gattaccacg ctggtagcct tcctgccaag caagacctcc aagtccacct gcgccgatcg
    18601 cgccggccat cgcagtaaat ccgatcatcg agatcgttgt aactgttaat ccagaaatca
    18661 atgcgggaat actttcaggt atcaagacct tccagatgat ttcccagtaa cttgctccca
    18721 ttgcatctgc tgcttccagt acaccagggc tgacttcacg aaaagcaatc tctactagac
    18781 gggcataaaa tggtgctgcg gataaaacaa gagctggaat ggctgcttgc gcacctagca
    18841 tagtgcctac gattgcttta gtaaatggaa tgatcaaaac catcaggatc ataaatggcg
    18901 ttgaccggaa aatattactg atgatcgata cgattccata aatgattcga gcatacggtt
    18961 tgtttttgcg tccaatagag taaaggatca atcccaaaac taatccgatg atcgtcacta
    19021 agaccattga gattaatgtc atatatagtg tatcaaggat agcctgattc atcgtttccg
    19081 ggttgatttg tttaaaatct agatatgttt ctgaaaagct tttatccacg atggatcacc
    19141 tcaattccta ctccttgttc tttgatgaac acttctgctt gatctaattc tttggtttca
    19201 ccagtcatca agacagtcaa tgaaccaaag gaaccttctt tcacttgttt gatctttcca
    19261 tacacaacgt tgacatcgat gttatagcgt cgaatcgctt gggaaatgac tggctcgttg
    19321 gcatttcttt cgctaaaggt caaagttgcc aaacggccgc ttggattttc tttgatgagt
    19381 tcagctaata gttctgtcga atcagcttct ggttcgatgt cttgttgtac aaagcgtttg
    19441 gttacttctt ttttcggttg tctgaagaca gtcaatacgt ctccttcttc tacgactttc
    19501 cccaactcca tcacagcgac tttgttgcag attttgcgga tcacattcat ttcatgtgtg
    19561 atcaagacga tggtcaaatt caatttctta ttgatcgcta acaataagtc taatacttct
    19621 tctgttgttt gcgggtccaa tgcgctagta gcctcatcac aaagcaggat ctcaggatca
    19681 tttgccaatg ctcgtgcgat accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga
    19741 tatgcttgtc cgcgtccttc taacccgact aaacgcaaca gttcttctgc ccgttcttta
    19801 cgtttgctct ttggtacgcc agctaattcc agcggcagct ggatattttc aagtacagtt
    19861 cttgaccata acaggttgaa atgctggaag atcataccga ttttctttcg gaatgtacgt
    19921 aattctttgt tttttaattt tgtaatatct tgatcgttta cgatcacttc gccgctcgtg
    19981 ggaagctcaa gtccattcag gagacggact aatgtacttt ttcctgcgcc agaatagccg
    20041 acgattccat aaatatcccc tttttcaacg tttagtgaaa cgtcattgac ggcttggatg
    20101 tttccgtttt tcccattgaa tgtctttttg acattcgtta gttggattaa tgacatgcca
    20161 ttcacatctt tctcttatga tttttcaaac aaaaaagcct tcgccctctt gccttaagca
    20221 aaaggacgaa agcttgaact tccgtgttac caccttgatt taccgttact tcgcaataac
    20281 agcctcttcc agtacttgga cttagcttct ttccgtatac tgtcgcgctg taacgggcac
    20341 tcccgtagta agtttgctta cgctcgctta ctctgctcta agaccatctt ccatctcatt
    20401 ttcgttaccc attttcagca aacagggctc tcttcaaacg tccgtgacat gtactcttct
    20461 tttcaaagct ttctcgtttc atataggatt gcttatgatt atagcaactg gaagaatcag
    20521 tgtcaacttt tttcaagtga ttttctgatt ttataattcg tcaaattctt ttggatcaac
    20581 atctttgaaa cttaagatcc acgcattcat ttcatctgaa tcattcaaga tattgatatc
    20641 ttcaccgatt ttatcattaa cagaagaaac tgtacccgtt agcgggctgg caaattcatt
    20701 gaccgctttt tccgcttcaa cttcgatcag aggttcatct ttttttagcg attgtcctgc
    20761 ttttggcaga ttggcaaacg tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc
    20821 aacacagtat tcttttccat taaataagat ccaaaggttg tcctttttct tcaaatgctt
    20881 gcctgccatt ttatatcagt tctccttcat aaattttttc attgaatcca attgcaacaa
    20941 gctggtcctc tcgtaagatc agtggtcgct tgatcagcat accatctgtc gataagactt
    21001 ggctcgcttt tgaagccgta aactcgccta cttgatcttt cagtccaagt tcccgatatt
    21061 tcatcccgct tgtgttgaac aggcgacgga ttggtacttg actcgtttct agccaagtct
    21121 caatcgttga ggccttcggt acttctttga tcatatcaac ggtttgataa ctaacgccat
    21181 gctggtctaa ccacgctttg gcttttttgc atgttgaaca tttcgggtac cagtaaaagg
    21241 tgtacaattt tcttacctcc ttgaagatct tgcaggtgct tcattttggt atctcatcga
    21301 taatatcaac ccgataccga tcatgttccc taaaatcgag gatcctccct gactaatgaa
    21361 aggcaatggg atccctgtta aaggaagtaa tccgatattt gctccaatat tctcaaagac
    21421 atggaacagc agcatcatga tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt
    21481 atcgaaacac acccgaatca tacgatagat taaaataaaa tacaagaaaa tgacgaatgc
    21541 accaccgata aagccgaagt tttcaccgat aacggtaaaa atcatatcgg actcacgaac
    21601 aggcacatag atattgctta cgttgaatcc tttaccaaac aagccaccgg taccaattgc
    21661 caaaagtcct tgggccggct gataactcat tcctgacgta tcatggaaag ggtccagcca
    21721 tgaatcaata cgtgcaaact gataagattt gaatccaact ttataaagca agtctcttcc
    21781 gccttcagtc gtaaccaaga agatcgttcc tgctccaacg actactgcca agattactac
    21841 aggtgcgatg atgcgccaag agattccaga catcaggaaa atcccgccaa agatcgcaag
    21901 aaagacaagc atcgttccaa agtcatcttg ggccatgatc aatgctaaaa ccggtattgt
    21961 cacgatcaag agcttcccaa tcagccaaaa gtccgatttc atcgtccgtt cttttacttg
    22021 cgtattatgt ttagttacga tcagtgccat catcaagatg tacgcaatct tcatcaactc
    22081 tgctggctgg aaagtaaacg caccgaatcg aaaccagttt ttagaccctg ttgaagcagc
    22141 taaattctga tcatagaatt tgagcaagag tcccattacc actaacccca aaccataaag
    22201 gtaaggagtc agtttccaga tccatttgga attcaaatgc atgatgacaa cgattgctgc
    22261 cgctccaact aaataccaga caacttgcat ccctacgcct ttcaagactg ctggattgtt
    22321 cggatcatgg ctaagtgcta catataatga gagcagacca atcaaacata acagaaagac
    22381 tggtaaaatc acgccataat caatacgatt atcgttcgaa gcttttaatt ttttttccat
    22441 gtttcatccc ttttcttaaa aaccatactg ctgattatta tagcggtcca gttagataat
    22501 ggaaagtctt tccatagaca tttgggaaaa attaactttc cgtttgtgca gttgcttgag
    22561 caaatgcagt acaaaagcct actgcttatc tttagtgtat catagaactt tttttgatac
    22621 atgcaattat ctaccagctg agattctcct cgctgtgaga agtttctgtg ctttcattgt
    22681 atcgaaggat ctcttcggca atctctagca gctgatcaga aaccagattg gttcgttgga
    22741 tgaaataaaa atatcgatgg aaattcaacg gagtcattgg cacattttct ggaacagagc
    22801 gcttagacaa aatcgagcga ccgattcctt ccttcagcaa cgcaacaatt acatcgttac
    22861 ttttcacgat catcttctta tcttggatat tcatctcttc aaaataacgc tgcgtgtaat
    22921 gaaaaacacc agagtttgct tctcttacaa gccacaattt gctttttggg tccccagcga
    22981 tgactagctg gtcttccatc aaagagtatc tttgtacgcc ggaagtctct aatggctttt
    23041 cgataaaacc aaaatcggat tcatgtttcg ctacttgttc taatgattca tgagaattcg
    23101 tgatttcgat ctcaaaatgc actttaggaa accttttgat cagatgtttc atcaactgag
    23161 gcaaaagata gacagcaaag gtgtgggaag caacgatccg acatacttct tcttcatttt
    23221 gttcattgag caatttttgc tgagtttctt cccattcttc taacaaaacc agtgtccgtt
    23281 catataataa gtcggcttgc ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa
    23341 gatcgatatt caattcccgc tccaactgtt tgatttgatt cgagactgct ggttgagata
    23401 aaaacagtac ttctgctgct ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg
    23461 ttaataactt aaacatggca ccacttccat aactttttat gataattact ataaaaacta
    23521 tttgatttaa atatacaaaa aaccatctat aatagaaatg tacaacaaaa cagttgctga
    23581 tccaactagt aacttagatg aagacaatca gctgtctgct tgctcgtaat catacattcc
    23641 tattcatttt acaatgtatt cgttcatctt ggtactaaaa tctattaaat aaaaaggaga
    23701 atattatata tgaagaactt tttgaaaatt attccgatcc cgatctgcgg gttgatcctt
    23761 gggttagctt cccttggaaa tctgtttaaa gactatcacc atgttgcttt aggcaatcta
    23821 actggcggta tctctatgat tcttatgata ttcatttttg ttaaattgtt tatcttattc
    23881 gaacacacga aacaaacgtt gcatgatcca atcatcgcct ccgtttcacc aacttttacg
    23941 atgtcactca tggttatttg tacttatttt gtttcatatg aaagtattgc accaattgtc
    24001 aaattcattt ggctgtgcgc tgttatcttc caagtgatcc ttgtctttta tttcaattat
    24061 caccatgtta tcaaagctga tctttcaatt gaagcaattt atccaagctg gtttatcatt
    24121 tacgttggat ttggggtcat cactgtaact gccgggaact tcttcccact tattggaaaa
    24181 attttctttt ggatttcatt gatctgctat gcgttcttat tgccgatcat catcaaacgt
    24241 gtctttttcg taagaaatat ggctcatccg acacgtccgc tgattacgat cgttgcagca
    24301 cctggttctc tttgcctaac aggttatctt aaaaactttg ctgaacctaa tttttatctt
    24361 gtcgtagcat tgttcgcttt atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca
    24421 cgtttgttca agcttcgttt ttatccaagc tatgcagcct ttacattccc gcttgttatc
    24481 tctgcaacag ctatatttac tactgttcat tacttcaaca gtttaggtat ctatagtagc
    24541 gtattggatt tcttaaaagt tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt
    24601 cttgctcatt acgtacgttt cttaattaaa gaacatagca aacatggaaa atttgtttct
    24661 aaaaaaatcg cttaataatc gattcaatag gttgtgaaaa agctgttctg catcaagtaa
    24721 taagaacggc caaacaaaaa cagctgttca tgtttttgta ctggttgggc ttattaccgc
    24781 agatgcaagc ttttgaacac cgtttaccaa gaggtcgtaa aagaagtggt cagcttcaag
    24841 aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatttc ggcttatttc
    24901 cgtagaagct acttctggaa caccgtttat tcgaaacaag aggtcgtaca agacttttgt
    24961 cacgacctct tgttcttttg tcaaaatact ggtttagtgc tactatgttc gagtatgctt
    25021 ttagtattta ttgacgaaag gaaccgatca aatgaaatca ttgtcacatc caataaaatc
    25081 tgtaaaggat tcagcggtcc gcgaaatctt acccggttta ctgctttctt ttgccatcgc
    25141 tgccttcagc aggcttcttg ctatttatct gccacagtta ggtggagcga ctattgctat
    25201 tttattgggt attttattag gaaatacttt ttttaaacag cctgttctag caatcggcac
    25261 aaaatttgct gaaagcaaac tattagaatt ctctgttttt ttactaggtg caactgtcac
    25321 agctcaaacg attgctcaaa ttggtgtaaa aggaattgtt tttgtgttac tgcaaatgtc
    25381 agtcactatc atcggtgctt acttgattgg taaaaaactg caattcagcg atacttcttc
    25441 tttgatgatg gctggaggaa acgctgtttg tggttcttct gctattgctt caatcgcccc
    25501 ttccatccat gcgaaagagg aagaaaaagg acagatcatc acactagtca atctactggg
    25561 tactgtgatg atgttgactc tcccttttct tggattacag ttgtttggtg gaaatctcat
    25621 tgaaaaaagt gcacttttgg gtggtacact ccaatctgtt ggtcaagtag tcgctggggc
    25681 aagcttactt gacaattctg ttgtcgaatt cgccatgcta tttaagattc tacgtattat
    25741 gatgctcgtt gttgttgtct atctgtttga aaaattcatg cacaagaaac aaccagctct
    25801 gtttgaaacg ccaactgata cgcagaaaaa acgaaaatta ccgcttcctt ggtatgtatt
    25861 agggtttgtg atcttttgtt tggtcaacag cttgttttct ttacctgtag ttattgacca
    25921 atcggcacat tttatcagca gctggtttga gatcactgct ttagcggcaa ttggtctgcg
    25981 tttagacttt gcaaaattca tgaaagaagg tacacgcttc ttatcttatg gattagctgt
    26041 cgggcttctc caaacagtga tggctttact atttatttgg ctgatcaaag tttagaatga
    26101 acatgaaaaa acagttgtga ttctcgcatg gaacaatgtc cgaatcacaa ctgtttttaa
    26161 ttttgcagat atccttctaa caacaacaat tggcgtttga tttcaacccc atctattgcg
    26221 tttcccatat atcctgtcag ttttccattt tgcccgatca ctcgatgaca aggaataacg
    26281 attggcaatg gattggttcg atttgcctgc ccaatcgctc gcacagcttt cggactatca
    26341 acagcaacgg ctacttcttt atacgttcgg atttctccat aaggaatggt cgacaaagct
    26401 ttccagacat tttgctgaaa aggcgtacct ctctttatag ataaaggaac atcaaattct
    26461 tttctttttc ccgcaaaata ttcttcaaac tgttcttgtg tgcgcgttaa aattcgctga
    26521 ttcgctgatt cgctttctga aattggctga aaagatacag cagtcaatcc ggattcatct
    26581 gcatctaacc ataaaaagcc gataggtgtg tgtattttca ttttgtttgc tcctagtcaa
    26641 tagactggaa cttcctcctt tttctctatc caaaactact tgccaactaa taaaaattgt
    26701 tcttaaaagt ttgataaacc gaggaaacat ggtaaactaa atacgttagt ttacaaaaag
    26761 aatacctgtt cttttacgta aaatcaaatt ttaaaggtgt tatttaaggt ttattaaatt
    26821 ttgaatgtaa accatatata aacatacgat tgaaaggata gaaacgatga aagtatcaat
    26881 tgttgtccca tgttttaatg aagaaaaaac tgttcctctt ttttttgatg aggttgaaaa
    26941 attcaaaggt gcccatgagt tcgaatattt gtttattgac gacggatcaa aagacggtac
    27001 gttagcagca atcaagaagc tagcctcagc ttatccttat gtccgctttg tctcattttc
    27061 cagaaatttt gggaaagaag cggctctgta cgctggattg caagcttcta caggagattt
    27121 agtgaccgtg atggatgttg atctgcaaga tccgcccgaa ctgcttcctg agatgatcaa
    27181 ccggatcact acttctgata tcgattgtgt cggcacccgg cgttcgactc gtgaaggcga
    27241 gcctgcgata cgcagtttct ttgcgaaaaa attctaccag ttgatcaata aaatcagcga
    27301 tacagaaatg gtcgatggcg ctcgtgattt ccgcttgatg actcgtcaaa tggtggatgc
    27361 cgtcctagaa ttaacagaat acaaccgatt ttcaaaagga ttatttagct gggtcggatt
    27421 taaaacagaa tatatttctt atgaaaaccg cgaacgtgtg gctggcgaaa catcttggtc
    27481 cttctggaaa ttattcaact actcgattga tggtatcgtg aacttctcgg atgcaccgtt
    27541 aacgattgct tcatttatcg gcgctttgtc ttgtgtcggt tctggtattg caattttgtt
    27601 cattattttg cgggcattgt tgtttggtga tcctacatca ggatggcctt ccatggtctc
    27661 gatcttcctg ttcattggtg gaatccaatt gttatgtctt ggaatcatcg ggaaatatat
    27721 tgggaaaatc ttcttagaaa cgaaacagcg tcctgtttat atcataagag aaacagaaaa
    27781 agtgaagcca aatgaattga agcctcacca ataatatacg tcaaatgaag taaacatttt
    27841 tttaaatgaa aaaggactgt gacaaaagtc ttgtcacagt ccttttttcc gaataaatgg
    27901 tgttccagaa gtagcttctt cggaaataag ccgaaatttc acaaaaattt gagaagcaat
    27961 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tatcacaacc tctttcatat
    28021 cttattactt cttcattgct gcttttagtg ctgctgctaa tgggctttct tctggttctt
    28081 ctgcttgtgc atattttttc agcaagcgtc gttcttcatg tttggtcatt ttcttcttct
    28141 gctctttttt atcaagcaat ttttctgtcg ttccatcgta tttacaacgg aaataagcac
    28201 cgtttttacc ttcgatgatt tccattttcc ggtgacattg cgggcatcga tgattagaga
    28261 cttttggatc ttttcttctg cggtagttgc aatctgaatt ggtgcagaca tagattttgc
    28321 cgtcacgtgt gtttttctcg cgcagattgg ccccacattc tgggcatttc ttttgggtaa
    28381 tcgagaaatc ttgatattct tgtttgcttg atttgatctc attcaccaag cgttttgttt
    28441 ctttttcgat tccattcaag aaggttcctg cttgtttctt tcctgcagca atagcttcaa
    28501 ggtctttttc ccagctttct gtcaaatctg gtgttactaa agagggattt accaatacta
    28561 ataattgctt acctttagga gatacttgca atccttgagg ggtacgttcc atcagttcag
    28621 atttgattag tttctcgata atctctgcac gcgtagctgg ggttcccaga ctgtattttt
    28681 ccatttgacc aagcaagctt gcttctgaca atggtttagg cggtgcagat agttcttttt
    28741 tgatggcgaa atcgggattg attctcattc ctttttccca tttgactgtt tccgtctcag
    28801 tttcagtttc gcctttccat cctgcttgga cgacacgatt ttgccgaaaa atgaatcgtt
    28861 ctttaccaaa ttcaaccgta acttttgttt ggctgacttt atggggttga gcaaacagtc
    28921 ctaaaaaacg agagacaatc atttgataga tttttcgctc ttcattactt aatttttcat
    28981 aacgtgctct gttttcagtt ggcaaaagtg catggtgatc ggtcacttta ctgtcttgga
    29041 atacttttgt ctgttggacg atggctccgt ttttcaaata ctgcttgacc tcaggagcaa
    29101 agtcgctgac tgcttgcaat cgttctttca tcgtactttt catgtcattt gtcagatatt
    29161 tgctgtctgt acgcggataa gtaacgatct tatgggtctc atacaaactt tgtacaagac
    29221 ttaatgtttt ctttgcagaa tagccatagc gttgattggc ttctctttgg atctctgtta
    29281 aatcataagg caatggcgca gattccgttt ttaatttttc ttggacttct gtgacagttc
    29341 ctttgcttgt actgatcaat tgaacaagtt tttgtgcttc ttcttgtgtt tttaaagcaa
    29401 attgattttt ttgaagcatt ttggcttgtt cttttccagc ttgcaacgtg atcgtgaaat
    29461 acgtttgtgg acggaactgc tcgattttct tttcttggtc tctcactaat gctagtgtcg
    29521 gtgtctgcac acggcctgct gataaattat ccgcgtactt cacagttaag gctctggtca
    29581 cattcaatcc taccagccag tccgcttttg cccgggcaag agcagattca tacaatgcat
    29641 catattattt cgcaggtctt agttgcttga atccagtttt gattgcttta tctgtttgag
    29701 aagagatcca taaccgcttc actggtttat tgaaatgcac atactctaag atccagcgag
    29761 caacaagttc cccttctctt ccggcatctg tcgcaatgac tgcttctgag acatcttttc
    29821 gctgagccag ctgcttgatt gcttttaatt gatgtcctgt tttaggaagt ggtttgattc
    29881 ctagtttttt agggatcatt ggcaacgtgt ccatttgcca gctctgccat tccttattca
    29941 gatcttccgg cattttcaat cctaataaat gaccaagggc ccaagtaacg atcaccttgg
    30001 gtccttcata ataatttttg tgcttttgat tagcaccaag caccttgctt aggtctttcg
    30061 caacgcttgg tttttccgct agaattagtt gtttcataaa attcctttct gctttctact
    30121 gtgatgggct gatgattttt caacaacgca agtcccttgt attgctgtaa atcatcgtca
    30181 cactcttcac accagcgctc atctccgtca ttccaaaagg cgctgagaaa gttacttttt
    30241 gcattgatat attcataatt ttgatggatt tcgttccatt gtttttcgta cgcttgtcgt
    30301 gcttcttcga aagactcgaa tgtttcttct tcttcaatat cttcttccca gtcttcaaaa
    30361 aaccaccaag gctcattgtc cccgaacatg gtaattactt gatacatact cgtgctcctt
    30421 ctttcagctt ttctagactc atcttagtca cttttcaaga aagttgcaag aattttctct
    30481 tataaaaggg tcatttaatg aacatcttaa aattgttaca aatgaatctg attgtttaag
    30541 gcaaagtagc gataaagtta ttgatcatcc aaaaatagct gtcttctgga tcgattgttg
    30601 gttcttttgt aaagtatcct aaaaaacgta aggaaagata accgtggatc atactacgaa
    30661 gttccctgct tcgatgaact ttttcttctt tcgttaattg aaaagcctca aatatctgca
    30721 aaatgatctg attcgtttca tggattccat ctaataaaac agcttcattg gtatgcggta
    30781 cactgatcag taactcatac actgcttgat ttgaaaaagc aaaggatcga taagtttctg
    30841 catagatctg cagtgcttcc tgaccacttt ttcccacaag agcccgtcgc agctggtcat
    30901 taagttcctg cagaagtttc gctgttagcg ctgttttgac atcacgaata tttttgaaat
    30961 ggttatacag cgagggagcc ttgatatcca agcgtttggc taaatgctga aaggttattt
    31021 gctgaacatc catctcttct gctagttctc gaaagcagtc gatgattttc tcttgagaca
    31081 gatttctttt tttcatttcc atgtttcctc tcataaaata aatccttcac tctttcagtt
    31141 taccacgaaa aagaaagaga aaaaagagac tttcattgat aaactacata ttgtagttta
    31201 taataaagag ctgtagatga aaattttcag aaagaaggag tctaattggc tgcaagaata
    31261 aaggaaaaat tatttaggaa aagagaaact tgggaacaaa acttgtttgt cttatggttt
    31321 ggaacattta tggctggtat cggatttagt ttagtcatgc cttttatgtc actttacata
    31381 gatacgttag gtaattatag tacgtcacaa ttgaactttt ggagcggatt gactttttca
    31441 tctactttct tggtgactac gcttatctct ccttggtggg gaaaattagc cgaccaaaaa
    31501 ggaagaaaat tgatgctgtt gcgtgcttca ttaggaatgg caatcgtcat ttctttgatg
    31561 ggttgcgtga ctagtgtgta tcaattagtc gcattgcgtc tgcttcaagg gattttttct
    31621 ggttatatca gtaatgccac tgcccttgtc gcaactggga cgccaaaaga aaaaagtggt
    31681 caagttcttg gaaccttagc aactggttct gttacaggaa cattacttgg accgcttctt
    31741 ggcgggatct ctgcttcagc gtttggctat cgccctacct ttttcattac agggatcatc
    31801 ttatttttcg tcttcttgtt gagtctgttt ttcgtccatg aaaaatttgt cccagtgaaa
    31861 aaagaaaaca tggtttctgc caaacagatt ttcaaagact tgaaatatcc acacgtcgtc
    31921 atcggtatgt tcgtcacaac gatgatcatc caagcatcca acaattccat tagtccaatc
    31981 atcagtctgt acattcgcca attgctgcat ggacatggga atgttacgtt gatcagcgga
    32041 atcattgctt cgatacctgg gattgccact ttgatagccg ctccaagatt tggtcgctta
    32101 ggtgacaaga ttgggagtga aagaatactt gcgattggtt tgggttttgc gattctggtt
    32161 tatatcccaa tggcattcgt cacgaatgtt tggcaattag ctgccttacg ttttttgatt
    32221 ggtatctctg atgcttgtct gctccctgct gtccaagcat tgatcacaaa atattcgcct
    32281 catcaagctg ctggacggat cttcagttat aaccaatctt tccaagcaac tggtaacgta
    32341 gtagggccta tgatcggctc cagtgtttcc agtgcatttg gttatcgtgg cgtctttctt
    32401 tcgacttctc ttcttgtttt ggtcaacttc ttgcttgtac gacacaatac acaagagatc
    32461 catcagcaag aaactgcaga agaaaaagct tcaggagtct ccatagcttc agggcactct
    32521 ccttctcata gacaataagc gtaaatgaaa aacactgctt gaacagttgt cacagactgt
    32581 ccttgcagtg tttttgttta ggcctgtttt ttcaagaact cagataagag gaataatccc
    32641 ttttccaagg tttctttttt acaacagtaa cctagtcggg catgcttcgg taaatcaaag
    32701 gcacttcctg gtactaatag tactcctgtt tcttctaata aattgataca aaatgtctgg
    32761 tcatcttctt cgatggctaa tttaatgaaa gaagtagaga cgtaattggg agcgacaagt
    32821 tcaactttcg gttcattggc tacccattgt tttaaaatca caaggttctc tgtaatgatc
    32881 tttttgtttc gcgccaatat tttttcttta ttcttcaatg catgtacagc aagatcatct
    32941 gacaggactc cgccgcaaat catcgtgtag tctcgatact ttcgaaaaac ttctgccaac
    33001 tcaggtccag tagctgtcca gccaatacga atacctggaa tggagtaggt tttggatagg
    33061 gaattggttg ctataccttt ttcatagaca tctacgatcg acaagaattc acctttatcc
    33121 gttaaaggcg catatacttc atctatcaat acatatgcat ctactgtacg agcaatttct
    33181 gcgatttttt ctaaagtttt ccgatctagc aatgtgcctg taggattgtt tgcactattc
    33241 aaacagatga tttttgtttc aggtttgact aaggcttcta gctgttctaa atcaaactgc
    33301 caatcttctt cttccttcaa atgaacaaag tcaacagcgg cacctaaaga tttggggatg
    33361 tcatataatt gctgataagt aggaagcatc gaaacaacat ggtctcctgc ttccactaac
    33421 gcgtatagag ctaaaagatt agcaccagtc gctccattcg tttgtaagat gttttccgga
    33481 tcgacggttt gataaagaga agcgacttct tgtttgaaaa catctgatcc ttcgatccag
    33541 ccatagtcca atggttcgtt tttgcgtttt tcgaaaaatt tacttacgct agtcccatct
    33601 aagccaacga gttcgtctaa tgtcaatgct tcgatcgagc tttgtgcaat atcatatacc
    33661 gctttctttt cccatttatt tagccattct tctacaccga aatttgcgat tttcatgttt
    33721 ctttccccct tatatcttga tccagcctac agcaagcaac ccgattgaaa gcagtgcggc
    33781 cacacaaatc agcccaatca ttactttttc ttttcccgta atttgatgtt tggtttcacg
    33841 acaagcgatc cagtagaagc aaaaaccagg aagataggca atactacata aaaggacata
    33901 agaaaatcct gctaaaatga tgcccatcaa ttcaaaaact acagcaatcc caccgatcac
    33961 tacttgtttt ttgattcctt cttttaaaga aaactggact tgatagatcc cgacaagtag
    34021 ataacaaatc aaaattgccg ctgtacataa agaataggca aattgatagg cataatcagt
    34081 aactaagaaa gtcaataaga atagattagt caagattgca gtaacaacta acgaataagt
    34141 tggggcattt tttttattcg tttttcccca acctttcggc aacagaccgt ctcgagccat
    34201 caacatcgtt gtttctgctg ggagcatcgt ccaagacaac caagaaccaa tgatcgaaat
    34261 aatcaagccg atattgatca gcgctgctcc ccaatttccc acgattcctt ttaaaatatt
    34321 tgccatagca ggttgtgaaa tagttgctaa ctcttcttga gataagacgc cgtatggcaa
    34381 gatcgaagca aggatataga taaacaataa acaaagcaga ccaatgatcg atgcttgctg
    34441 ggcttctgaa cgtttcttcg cgcggttggc taaaacactt gcgccttcga tccctacaaa
    34501 gacccacatc ataaccatca aacaaccttt aatctgcaac cagatcgttc cttgctgccc
    34561 tgttccattc actagattat cagatacgtt accccaaaaa tcagcagtga atatattcac
    34621 tttaaatgcg ataaatacaa atattaaaaa taggaataat ggcactaatt tacagatcgt
    34681 cacgattgta ttgatgacgc tcgctccttc aactccattg tttactaata aagtcagacc
    34741 ccaaataaaa atacttgcaa ataaaataga aggaacattt tgtccacctt tgaatatcgg
    34801 gaaaaattct cccatggcgc tcatcagcat cgttgcaaaa gcaacatttc ccagccacgc
    34861 agacaaccag taaccccaac cactgatgaa gccacctaat ttaccaaatg aagcttccgc
    34921 gtaaccgaaa ataccaccat ctagatctgg acgcttttct cctaaatgat tcagcgataa
    34981 taccaaagct aaaataccaa tcccaacgat tatccatgaa aggatcgctg gccctggagc
    35041 tgcagaacta gccagatcac ttgtgatccc aaacacaccg cttccgattg acgaactgac
    35101 aaccagagca accagtcccc atttgctaat acccgtcgat ttcttttcca tttccatcac
    35161 cctcacttat aaaattgaac aaatcacttg caaaagattc ccttctattt tgcaactgct
    35221 cttgcttaac aaaaagcaac tttattcata ttattggata ttatgaactt tttacacaag
    35281 tatttcaagt ataaaatgaa taaaaattaa ttttattgca aaaaaacagc tatgctatcc
    35341 aaaagaaaag gtgtttcttt tgttagccat agctgttttg attcatttaa tatcgcttaa
    35401 acgtattgta aaacttttgt ttcttgatat gcgtggtcga tcaaaccacc acccagacat
    35461 tccattccat cataaaatac aactgcttgt ccaggagtga ttgcacgtac aggttcgtcg
    35521 aatacgactt ctgcacgatt atcatccaat aagcgaactg tcacaggaac gtcttgctga
    35581 cgataacgga atttcgctgt acacttgaat tcttttggca ttggttcatt cgtcgtaaag
    35641 tggatttcac ttgcatccaa gcttgtggca tacaatgcag gatgatggaa cccttgcccc
    35701 acatataatg tgtttgtcgc tagatctttt cctacaacaa accagggttc ttgcgaatct
    35761 cctccgccac cgatccctag tccttgacgt tgaccaatcg tataatacat caagccagca
    35821 tgttgtcctt tgacttcacc atcaagagtg accatatttc ctttttttgc tggcagatag
    35881 ttgctcaaaa attgtttgaa gttcttttca ccaataaagc agattcccgt tgaatctttt
    35941 ttcttagcag tcgcaaggcc tgcgcgttca gcaattgccc gtacttctga tttttccatg
    36001 ccgcctagag ggaacatcgt ttttgaaagc tgttcttgtg ataattggct aaggaagtaa
    36061 gtttgatctt tattattgtc tactccacga agcatgtgga ccacgccgtt ttcatcgcga
    36121 gtgacttgcg cataatgtcc agtagctaca taatcggcgc caagatccat tgcataatcc
    36181 aagaatgctt tgaatttgat ttctttgttg cacatcacgt ctgggttagg tgtacgtcct
    36241 gcgcggtatt cggctaggaa atattcaaat acacgatccc aatattcttt ttcaaaattc
    36301 acagagtaat aaggaatccc gatttgagca gctactttcg ctacatcctt ataatcttcc
    36361 gtggccgtgc atacaccgtt ttcgtccgtg tcatcccaat ttttcatgaa tattccgatc
    36421 acgtcgtatc cttgttcctt taacaaaagt gctgttacag atgagtcgac accgccgctc
    36481 atccctacga caacgcgtgt tttgctgttg tctgtcatga tatcaccatc atttcaatta
    36541 gattctgaaa catctacgag ttgaaggtcg caacttttca gtattttaca ttatacgtca
    36601 tatttctgaa aatttcatcc cttaattatc aagtcgtaaa aatactgttc tactggacct
    36661 attcagtaca caaacatgat agtcaaccaa aaaatcactc atcttttgag cattcgatat
    36721 aaaaaattgg catactcata aaaagggcca gcctttcggc aataaggaat aattttataa
    36781 attgtgaaaa actatttttc aaaattgctt cttatcgctt aaagctgcac gtctcattta
    36841 caaccttttc tttttagatg ttatgagttg ctcagataag tgattttgtt tactctaaga
    36901 aaaaagggag gcaaaagata catgaattac tttatcagtg atatgcactt tttccatgaa
    36961 cgcttattag gaaaaaacga ctttgctccg cggctctttg caaatgtgga tgaaatgaac
    37021 caacagttga tcactagctg gaatgcagta ataaaagaaa ccgaccatgt ttatcatcta
    37081 ggcgaccttg ctatgcatcc gcaatatgaa aaagcaaatg cagaaatttt ggaactggtc
    37141 actcaactgc aaggaacaat ccacttgatc aaagggaacc atgacagtcg cgcttttttc
    37201 cgttatttag aaaaaaatga tcctaggctt actgacggaa gtcccaaatt ttatttttat
    37261 gatgtaggtg ccatcgttaa attcgaccat caacaatatt acttgactca ttatccgatg
    37321 ttgctcgggc ctaacgacac tatccgcaat cttcatggac atatccatca ttacagcgta
    37381 cctatcgaga acgatgtcaa tgtaggcgta gacgcaccgg aaagagaact cttaaaggaa
    37441 aaactaccat ttggcactcc gttatctgag caacaaatca aagaaatcta tgaagcaaag
    37501 aggaagtacg ataggtcgtg aaaaaactgc cctatatcaa gtattaaaca aataaagata
    37561 attactcata tttttcattt gagtaggctt attgccacag gaacattagg atcagaaatg
    37621 gagttgtaac aagacttctg tcacaactcc attccatact attcgtcttt ttgctcatgg
    37681 tgttctttta ccagtttttt ctgtaattct ttatcataat ttcgggcagc tgacgttaaa
    37741 aataaaacga ttccgcataa taaaatcccg attccggaaa acaagaacat tttttctacc
    37801 cctaaacgat cagctaatgg tcctgcaaat aataatccaa ttggacctgc caggttcatc
    37861 aaagagttga agttccctaa gacacgacct aaaacattcg attcataact ctgctggatc
    37921 attgccatca gcagtgtatt gaaatatggc gttgcacaac ctgctcctgc attcaagata
    37981 agaaagtaca agaacccttg actatcgttt ggaaccaatc cgcttgcacc gatcgtcagg
    38041 cccataatga tgtaggctat aataataggt ttcattcggt ctttccatgt tcctataaaa
    38101 ctgatcagtg caccgcctaa gagcattccg actgcgtaga ctacttccac tgctcccgcc
    38161 tgtccaacgg aaccaccaaa gtaatctagc gtcatcaatg gatacagact gattgctggc
    38221 atgaacaaca gcatcacaaa tgcaccattg atcgttatgt accataaccc tttattatcc
    38281 atcaattgct gcagcccaaa ttttgcatct ttaaaaatag taagcggaac ttccaatatt
    38341 tcaggagaaa cttttgggat cttcacaaaa atcaataagc cgacaccaaa aactgccccc
    38401 aatacatcta ataaaataag gtaattgaca ggaacgactg aaaacaaagc cgcacctaga
    38461 gctggcgcaa tgatgaaatt cgctgattgg accattccta actgtccatt cgtccttgtt
    38521 atgtgagaag atggaacgat tgtcggcata atcgactgga tcgttggcat ttggaatgtt
    38581 tgtgcgaccg aacgcataaa caaagacaca aaaactagcc aaatcgggaa ggattctgaa
    38641 atcgttccga ctactgctaa aataagcgcg aaaatcgcta caataatatc cgtgacaatc
    38701 aaaagagctt ttttatccca gcgatccact agcggtccta caaatgggct tagtaaaacc
    38761 atgggaatca ttcctagtaa agtagcaaaa ctcaaaattg tcgccgaacc ggtttctctt
    38821 gtcaaatacc agatgatcgc atattggacg accatactag tgattcctga caaaaactgt
    38881 cccgataaaa agaacaaata atttctcttc caattcgtta aaacgaccga ctgttcattt
    38941 tccataaaca tccctctttt ctttttcatt ttgtatagcg tttgcacaac aatcattata
    39001 cgggattctt ttttcaaaaa atac
//
LOCUS       DAHHPA010000015        36860 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.016, whole genome shotgun sequence.
ACCESSION   DAHHPA010000015 DAHHPA010000000
VERSION     DAHHPA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.016"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            539..1774
                     /locus_tag="NBH55_000716"
                     /pseudo
     CDS             539..1774
                     /locus_tag="NBH55_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            1782..2669
                     /locus_tag="NBH55_000717"
     CDS             1782..2669
                     /locus_tag="NBH55_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1508723.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            2686..3522
                     /locus_tag="NBH55_000718"
     CDS             2686..3522
                     /locus_tag="NBH55_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD1508724.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            3525..4148
                     /locus_tag="NBH55_000719"
     CDS             3525..4148
                     /locus_tag="NBH55_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508725.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            4145..5437
                     /locus_tag="NBH55_000720"
     CDS             4145..5437
                     /locus_tag="NBH55_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1508726.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      5796..5893
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            6009..6590
                     /locus_tag="NBH55_000721"
     CDS             6009..6590
                     /locus_tag="NBH55_000721"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCD1508727.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            6593..7900
                     /locus_tag="NBH55_000722"
     CDS             6593..7900
                     /locus_tag="NBH55_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCD1508728.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            8193..8681
                     /gene="purE"
                     /locus_tag="NBH55_000723"
     CDS             8193..8681
                     /gene="purE"
                     /locus_tag="NBH55_000723"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCD1508729.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            8674..9798
                     /gene="purK"
                     /locus_tag="NBH55_000724"
     CDS             8674..9798
                     /gene="purK"
                     /locus_tag="NBH55_000724"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCD1508730.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            9811..11106
                     /gene="purB"
                     /locus_tag="NBH55_000725"
     CDS             9811..11106
                     /gene="purB"
                     /locus_tag="NBH55_000725"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCD1508731.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(11286..11663)
                     /locus_tag="NBH55_000726"
     CDS             complement(11286..11663)
                     /locus_tag="NBH55_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508732.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            11919..12647
                     /locus_tag="NBH55_000727"
     CDS             11919..12647
                     /locus_tag="NBH55_000727"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCD1508733.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            12647..12901
                     /gene="purS"
                     /locus_tag="NBH55_000728"
     CDS             12647..12901
                     /gene="purS"
                     /locus_tag="NBH55_000728"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCD1508734.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            12903..13574
                     /gene="purQ"
                     /locus_tag="NBH55_000729"
     CDS             12903..13574
                     /gene="purQ"
                     /locus_tag="NBH55_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCD1508735.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            13575..15797
                     /gene="purL"
                     /locus_tag="NBH55_000730"
     CDS             13575..15797
                     /gene="purL"
                     /locus_tag="NBH55_000730"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCD1508736.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            15782..17221
                     /locus_tag="NBH55_000731"
     CDS             15782..17221
                     /locus_tag="NBH55_000731"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCD1508737.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            17244..18296
                     /locus_tag="NBH55_000732"
     CDS             17244..18296
                     /locus_tag="NBH55_000732"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCD1508738.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            18293..18871
                     /gene="purN"
                     /locus_tag="NBH55_000733"
     CDS             18293..18871
                     /gene="purN"
                     /locus_tag="NBH55_000733"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCD1508739.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            18868..20403
                     /gene="purH"
                     /locus_tag="NBH55_000734"
     CDS             18868..20403
                     /gene="purH"
                     /locus_tag="NBH55_000734"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCD1508740.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            20419..21693
                     /gene="purD"
                     /locus_tag="NBH55_000735"
     CDS             20419..21693
                     /gene="purD"
                     /locus_tag="NBH55_000735"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCD1508741.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            21815..22729
                     /locus_tag="NBH55_000736"
     CDS             21815..22729
                     /locus_tag="NBH55_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1508742.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(22862..24427)
                     /locus_tag="NBH55_000737"
     CDS             complement(22862..24427)
                     /locus_tag="NBH55_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCD1508743.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(24420..25871)
                     /locus_tag="NBH55_000738"
     CDS             complement(24420..25871)
                     /locus_tag="NBH55_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1508744.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(25885..26901)
                     /locus_tag="NBH55_000739"
     CDS             complement(25885..26901)
                     /locus_tag="NBH55_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1508745.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            27121..28209
                     /locus_tag="NBH55_000740"
     CDS             27121..28209
                     /locus_tag="NBH55_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCD1508746.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            28263..29789
                     /locus_tag="NBH55_000741"
     CDS             28263..29789
                     /locus_tag="NBH55_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCD1508747.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            29791..30996
                     /locus_tag="NBH55_000742"
     CDS             29791..30996
                     /locus_tag="NBH55_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1508748.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            31253..32230
                     /gene="guaC"
                     /locus_tag="NBH55_000743"
     CDS             31253..32230
                     /gene="guaC"
                     /locus_tag="NBH55_000743"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCD1508749.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            32494..33918
                     /locus_tag="NBH55_000744"
     CDS             32494..33918
                     /locus_tag="NBH55_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCD1508750.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            34379..35488
                     /gene="pdhA"
                     /locus_tag="NBH55_000745"
     CDS             34379..35488
                     /gene="pdhA"
                     /locus_tag="NBH55_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCD1508751.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            35491..36468
                     /locus_tag="NBH55_000746"
     CDS             35491..36468
                     /locus_tag="NBH55_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCD1508752.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            36493..36741
                     /locus_tag="NBH55_000747"
                     /pseudo
     CDS             36493..36741
                     /locus_tag="NBH55_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
BASE COUNT        11739 a         6251 c         8287 g        10583 t
ORIGIN      
        1 ttgttattat tagcattcta acaaaaaaat gtcttgattt attgaagcgc tttctataga
       61 atgaaaaacg aataaaaact aaaaaaggca cccgacaatt tagtagcagc taaattgtcg
      121 gggcttgaaa agtcagcggg aactgaccaa tcaagttgcc atagtcaatg cttgtgcatc
      181 gacgtgtata ttgtacctaa tttcatacag aattactagt tatttttctg ttttttaggg
      241 aagagcgcac acgaagaagc atgcttattt agctatgtca aggatattgg gcagttgtct
      301 gatatccttt tttctttgcg taattatcct cccatcattg aagcaagaag tgtttttagc
      361 gatgaaagaa gaatcagcac tatctaattt ttcttgaaag tattgagttg ttcatcctag
      421 gcagaacgat aaggcaagtc ttggttttta ttccccttga aaaagatcgt ttaataaata
      481 tgttctgtat aaaaatacag tatattgaaa aataacaatg gaagtagagg tgcagagaat
      541 gaagaaaaga atgagaatca tcttagcttt aagtgttatg ttattcttct tgacggcttg
      601 tgggcaagaa gatgacaaaa aaatcacgct gaacatggct tggtggggtt cgcaagtccg
      661 acacgatgca acagtaaaag taatcgagct ttatgaagaa aaaaatcccc atgtgacgat
      721 cgattatgaa ttttatgatt atgaaggata ttacacaaaa ttaggtacac tagcagcatc
      781 agatgatatc tgggacattt tccaactgaa caatcaattt cctcaataca tcaatcagat
      841 tgaaccatta gatgaatatg tggagcaagg gataattgat atttctaatg cagatgaatc
      901 ctatttagca acgacgactt acgacgggca attagttgga ctgtctaacg gggtgaattc
      961 gtttgcgatt gcttacaata aaaatatatt tgaacaatta aatatccctg aaccagcttc
     1021 aaattggaca tgggatgaat ttgaacaaat cagctatcaa atcactgaag aattaggaat
     1081 ctatgctatt tcaagattcg aagattttat agctggctgt attattcaaa taccacaagt
     1141 tgagaaagga ttgaattttt ataatcggga agatctatct aacagcttgg gatttgaaaa
     1201 tccagactat ctaacagatt ttttccagct gcgtaaaaat ctggtagaca aaggggccca
     1261 tccagaacca ggtggtggag ctgcaagtac gacggatctg tcttttcagg cggttaggga
     1321 tagagaagcg gcaatggtgt ttctatcaag taaccagttg acagaaatat tgtcaggggc
     1381 tcctgaagat atggagattc agttgattaa tccacctcgc agaaaagcag atggagaatc
     1441 tggtatccct ttacgttctt cgcaaatgct gagtatggca aaaagctcag cccataaaga
     1501 agaagctgct aagtttattg atttctttca aaacagtgaa gaagccaatg aaattttaaa
     1561 aggagaacgt ggtgtaccaa tcatgtcgaa tattcgagaa cagctcgctc gtgcagatga
     1621 tgcaactttg caagcgacat ttgattattt agaaatgatt tccgatatgg acaacggcga
     1681 agtgaatgtc tttgatagtg ctttgaatcc ggaaattcaa gaccaataca atcttttagt
     1741 tgaaaaagtc atttatgaag aagtaacagc agaaggaagg catgaaaaaa gaaaaactaa
     1801 aacggttctt ggatcaagac aatgtggtcg gttacgtatt tgcttctcca ttcatcctag
     1861 gatttctatt gataacagtg ataccgatga tcttgtcact ttattattcg ttcaccgatt
     1921 atcgtttggg aaatccagtc tcatggatcg gcttggataa ttatataaag atgctgagtg
     1981 atgcccgttt tttgaactcc atccgagtca cttttctcta tgtatttgca tctgtacctg
     2041 tgaaactgat ctttggtcta ttgattgctt atcttttaac gaggaatatc aaaggaacga
     2101 ctttctttcg ttctttgtat tatgtgccta gtttgatcgg tggcagtatt tctatcgctc
     2161 tagtttggcg ggagttgttt tctaaaaacg ggttagtgaa tattttcctg gaacgattag
     2221 gcgcacctgc gctttcctgg tttagcgatc aaaaactggt catgatccct cttgttttga
     2281 tgtctgcttg gcaattcggt tcggcaatga tcatctttgc tgcaggtatc aaagagatcc
     2341 caggctcgta ttatgaagcg gcagagatcg atggagcgaa caaatggcag acgttttaca
     2401 agatcacgtt acctcttctt tcgccgatca ttttgtataa cttggttatg cagacaatcg
     2461 cggcgtttat gacttttacg caggcattta tcattaccca aggtggtccg aatgatgcca
     2521 cgaactttta tgctctttat gtgtataacc aagctttttc ttttaattct atggggtatg
     2581 ctagtgctat ttcgtggatc atgctgttga ttatgggagc tgtcaccttt ctgattttca
     2641 aatcatcaaa aaaatgggtc gttgtgtgaa tgagaggagg aagaaatgaa taatacagta
     2701 acgaaatggt ttcaatacgg ttttattttg ctattaggtc tagtcatgat ttatccggtc
     2761 ttatggatga tcgcaggttc ttttaaaacg gatcaagaga ttatgagtgg ttcgctgaat
     2821 ttgattccag agacttttca atgggaaaat tacgcaaaag gatgggcggg gtttgctgga
     2881 atttcatttt ttactttctt taggaattcg tttgtcatct ctgcagtttc gacgataggg
     2941 acagttctga gctcgacttg tattgcttac gcattatcga gaatcgattt caaaggaaaa
     3001 agattctggt tcgtcgtcat gttagtaacg atgatgattc ctgcacaagt gattttgatt
     3061 ccgcagttta ttatttacaa tcggctgaat ttggttggga cgtatgtgcc gctgatttta
     3121 ccacattttt ttggacaagc atttttcatt taccagatca tgcaattcat ggtcagcatc
     3181 cccaaagagt tagatgaatc agcgattatt gacggatgca gcaagtatag tgtctttacc
     3241 aaaattattt ttcctttgct gaaaccttcg atcatcacga cgatcatcat ccagttttat
     3301 tggaaatggg acgacttcat gggaccattg atttatttaa acaagccgag atcttacacg
     3361 gtatcgattg ccatcaaatt atttgcagac gctggatcta gtacgactta cgcgagtatg
     3421 tttgcgatgt ctacgttgtc gctgctaccg gtttttctga ttttcttatt cttcaataaa
     3481 tatcttgtcc aggggatcag tacaagcggt ctgaagggct gaatatgaaa aagccaatag
     3541 aagagatgtt tctttacaag ctcaactact atgtctgtcg gattttttta accagtctta
     3601 tcagtaatgc tcttttcgct ttagttctag gatgtttgtt cactttagga ttagtgctgt
     3661 ctgctcgtgt tgtctatctg atttattttt tgatgatgta tccttcttat tatctatctg
     3721 tccattactt ggcagaaaaa aaggtatgtt ggaggaaagg gaaaatcaaa cggatgtgtt
     3781 tcatcattac gttgtttcag ctgtttgtca ttatttttgc agttgattgg tatttattag
     3841 tgggaaagat tggagcttgg tatctgattc cttttctatt gtgtctgttg ctttttgtat
     3901 cccatagttt ttttagtacg ctaattgata tctgcaaaga agataaagca gatagtcttg
     3961 tttatttgat caaaaggaat atctggctgt tgtcaaaaaa cgtcaagggg tttctgtggt
     4021 atttatgcct tttgtatgga ctgttctggt gtatacagcc tgatttttta ctcactgcat
     4081 tactgtatcc agggctgcca tttgtcacag tattttgctt aaaagaaaag gagaatgact
     4141 ttaaatgaag aaaaaatatg tacaagtcgg acttggtggg agagcgcgtt ttttttatga
     4201 ggcaattgcc tctacgtatc atcaaacttg tgaactagtc ggtttttgtg atgtatctca
     4261 aacaaggatg gattatgcaa atcggatact tgtgaacaag tatggacaga aaaaagtaaa
     4321 gacatgtatg ccatcacaat ttgagcaact actggatgac gtacggccgg ctgaagtcat
     4381 tattacgtca gtagatcgaa cgcatcatga ttatatcatt cgtgcaatga aaaaaggata
     4441 tgatgttatt tgcgaaaaac ctctgactat cgatgaggaa aaagctcaag aaataataac
     4501 ggtccaaaaa cagacgggaa gaagcctgcg agtcactttt aattatcgtt atgcaccgta
     4561 tcacacgaag ataaaggaac tgatagaaag tgatgtgatt ggtgatgtgt tttctatcca
     4621 cttcgaatgg ctgttaaata cgcaacacgg ggcagattat ttcagaagat ggcatcgaaa
     4681 taagcacaat agcggcgggc ttcttgttca taaatctacc catcattttg atctagtgaa
     4741 tttttggttg aacagccgtc cccaaaaggt atttgcattc ggtggattaa acttttatgg
     4801 gatgcataat gcggagaaac gcggagtaga aatattttac agtcgatgct atggaagcga
     4861 actagctaaa gctgatccat ttgctataga cctgtcagcc aatgaggaat tgcgagaaat
     4921 gtatttagaa gcagaaaaag acagtggata tattcgtgac cgcagtgtat tttccgatga
     4981 tatctctatt gaagatacac taggcgtaac ggtcaaatat gaaactggtg cgattttgac
     5041 gtattcgcta aacgcgtata tgccgtggga aggattcaat gtttctttca atggcagcaa
     5101 aggacggatc gaaatgaaag tgatcgaaaa gtcttacatc aatgcaggtg gagataaaaa
     5161 ggatgaagga gcagccgttc ttcagtccat ccatgtctat ccgatgttcg gtgagccata
     5221 tgaggtaatc gtcgaacaag aagcgggcgg acatggcgga ggagatccgc gtttattaga
     5281 tgatctattt ggtgaagaaa aagaagaaga tccgtggaat cgggcagcaa ctcacgtaga
     5341 tggtattttg tcgattttga caggaatcgc agcaaatcat tctattgctt caggaaaagc
     5401 agtagtgatt gacgagcttg tcagcttaaa agaataagaa tagatttacg gaaattactt
     5461 cccgtagttt ataaaatctt gccttattgc cgataggctg tcttttgaac actatttatt
     5521 cggaaaaagg ggctatgaca tgctttttgt catgccttct ttttatgtgg gctgcttttt
     5581 tagataggag aaaataaaaa taactattta aacccgaaca aaaaggaata atgcattaaa
     5641 aatcattaga ttttacaaaa aaataagtaa accacgttgt cagtttcaga aagtagtgtt
     5701 agactgtttt caatatttga aacaacattt tttattcaaa tatggcaaac tgccttcacg
     5761 tgcaaaacag ttgcaagaaa atgaatttac actattatta gttttacata cctacttata
     5821 tatcgtcata atatggatga cagtttctag ccagtaccgt aaatgctgga ctataagtaa
     5881 aagattggct atttcaccct tcttttctta tgataaagtg gagggtgttt ttctttaaca
     5941 gaaacatagt aggtttttgc acaaaagcgg ttatttcggt attaataaat gataaaggag
     6001 caaataaagt gaaagaatta gttgaacgta ttcaaaaaga cggacgtata ttaggcgagg
     6061 gcgtattaaa agtcgacagc ttcgtcactc accaagtaga ccctgtattg atggaacaaa
     6121 tgggaaaacg gtttgcagaa gtatttaaag aacaaggaat cacgaaagtt gtgaccattg
     6181 aagcttctgg gatcgcacca gctttatttg ctgcaagaga attagatgtt ccaatgatct
     6241 tcgctagaaa agcaaaaagt ttgacgatgg atgaagaatt attgacagct tctgtttatt
     6301 cttttacgaa acaagtgaca agtacgattt ctatttctcg taaattttta tcttcagaag
     6361 acaaagtatt gattatcgac gatttcttgg caaacggaca agcggcaaaa ggattgatcg
     6421 aattatgcca gcaagctgga gcgcaagtag aaggaatcgg gatcgtcatc gaaaagtctt
     6481 tccaagatgg acgtcagtta cttgaagaaa tgggattgag agtcgtttct cttgcgcgta
     6541 ttgcttcctt gacgaatgga caagttgagt ttcttgagga ggacgcataa tcgtggaaaa
     6601 agaaacacaa aatggaaaag ctgcggtttt agggctgcag catctgctag cgatgtatgc
     6661 aggagcagta gcggtgcctc ttttgatcgg tacaggacta ggctttaatc aagaacagat
     6721 gacttatttg atttcaattg atatctttat gtgtggatta gcgacattat tgcaattgac
     6781 agttaaccga tttttcggaa ttggcttgcc tgtcgtttta ggatgtgcga tccaagcagt
     6841 agctccactg atcttgatcg gaaccaatga aggagtagga gctatctacg gatctattat
     6901 cgcttctggg atctttgttg tgttagtttc tggtgtcttt tctaaaatta aaaaactatt
     6961 ccctcctata gtaacaggaa cggttatcac cgttattggt ttgactttga ttcctgtagc
     7021 aatcgagaaa atgggtggag gtaatgcaac agcagctgat tttggtgaca aaacaaatct
     7081 gctacttgca tttgtgacga ttctcctaat tgtaggcgtg caaatgctgg caaaagggtt
     7141 cattcgttca atcgcggtat tgattggttt agtcggagga agcatcttag ctgctttcat
     7201 gggaatgatt gatctgagcg cgattggaga tgcgccgttg ttccatgttc cacaaccctt
     7261 ttattttggt aaaccaactt tcgacgtatg gtcgatttta ctaatgatca tcatttccat
     7321 ggtcagcatg gtagaatcaa caggtgtata ttttgcactt ggtgacatta ctgggaaaaa
     7381 tgttggagaa gaagaattga aaaagggata tcgtgcagaa ggacttgccg ttatcttagg
     7441 cggtattttc aatacattcc cttatactgg cttttctcaa aatgtcggat tggttcaact
     7501 ttcgggaatc aaaacaagaa aacccatcta tttctctgca ttcttcttga ttgttttagg
     7561 attattccct aaaatcggtg cgttagcaca aatcatccct gagccggtac ttggtggagg
     7621 aatgttagtc atgttcggga tggtggcagt ccaaggaatc cgtatgctgt tggaagttga
     7681 tttcagcaat gataaaaatc tattgattgt tgctgtttct gttggatttg ggctgggttt
     7741 caatgttacg ccgacattat tcagtcaaat gccgcaaaca ttgcaaatgt tcactggaaa
     7801 tggcatcgta atgagtagca ttacggctat catattgaat ttgatcttta atggattgaa
     7861 aaaagatgaa gaaatattgg aagaagcctt caagaagtaa tagagcaaaa gaaaggttgt
     7921 gaaatcagcg ttttgacctg attgttagag aaacaaacgg aaaaatagtt cctcgtattt
     7981 tgccgtttgt ttgagctaat accgagggtc agctgattga acaccgttta tccataaaaa
     8041 ggaatgtgac ctaacttttg tcacattcct ttttttcatc tttaataaac acgaacaata
     8101 aaatgttttt ctttgttaac attcgaaatt tctttgacat caaaaaaagt gattgttaaa
     8161 atatgaatgt agaaaaactg ggaggaaaaa caatgacagc aaaaatctct gtgattatgg
     8221 ggagtacttc agattgggaa acaatgaaag aagcatgtca aatattggat gaattcggtg
     8281 tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc agatttgatg tttgaatatg
     8341 cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc aggagcaggg ggagccgcgc
     8401 atctgccggg tatggtcgca tcaaaaacaa ctttgccggt tatcggtgta ccggtccaat
     8461 cacgaacatt aaatggtttg gattcgcttc tttcgattgt ccaaatgcct ggaggagtac
     8521 ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa tgctggacta cttgcgatac
     8581 agatgctttc catgtatgat ttagaattgg aggctaaatt ggctgaaaga cgttcaatgt
     8641 tggctaaaac tgttatagaa agtagtgatc aacttggtta aacctttgtt gcctggttca
     8701 acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga tggcgattag tgcaaaagaa
     8761 atgggcttta aagtaggtgt gctggatcct gtgagcgatt gtccagccgc acaagttgca
     8821 gattggcaca ttgaaagtgt ctatgatgac acctttgcgc tgaaagagct tgccagaaga
     8881 acagatgtca ttacctatga attcgaaaac gtcagtgtag aggcgttaaa tgcgatttta
     8941 cccatgtcat ttattcctca agggacagac ttgcttgcga ttacgcaaga ccgcctgtta
     9001 gaaaaatctt ttttggaaac gaataacatc gtgattgctc cttatgcaac gatcgtcagt
     9061 ccgacggata tccaagatgc gattgacggg atcggctatc cttgtgtttt gaaaaccact
     9121 cgaggcggct atgatggcaa ggggcagtat gttttgaaaa gtcgggcgga tctagctcct
     9181 gccatggacc ttttaagaga aggaacctgt gttttagaag catggatccc gtttgaaaaa
     9241 gagatttcga tcatggtggc aggaaatggt caaggagatt tcacgacttt tccggtcgtg
     9301 gaaaacatcc accacaacaa tattcttcat gaaacgattg ctccagccgc gattgatcag
     9361 gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg cagaagcagt gagcctttca
     9421 ggtgttttag gggtagaaat gtttttaaca aaaacaggcg ggctatatgt gaatgaatta
     9481 gctcctcgtc ctcacaattc tggacattat tctattgaag cttgttctat gagtcagttc
     9541 gatgcccata ttcgaggtat ttgtggttgg ccattaggag aaaaagtacg tttgataagc
     9601 aatgcagtaa tggtcaatgt tttgggagaa caactgctgg aaagctatcg cttgattgag
     9661 aataagccag actggcaatt ccatttctat ggaaaaaaac aagcaaaaaa aggtcgcaag
     9721 atgggacata tcactatctt gacagaagac atccagcaaa cgctggaaga aatcaaacaa
     9781 acgaatattt gggattagag gagaaaaaac atgttagaac gttatacacg ccctgaaatg
     9841 gggaatatct ggacggaaga gaacaaatac cgtgcgtggc tcgaagtcga gattttagca
     9901 gatgaagctt gggcagaact tggtgaaatc ccgcaagaag atgtgaaaaa aattcgtgag
     9961 catgcatcat ttgatatcga tcggatcttg gaaatcgaaa aattaacacg ccatgatgtc
    10021 gttgcgttta cacgagcggt ttctgaaaca ttgggagaag aaagaaaatg ggtccattat
    10081 ggtctaacaa gtacggatgt cgtagatact gcttatggct atctcatcaa gcaagcaaat
    10141 gatattttac gggaagactt gaaacgtttt acggaaatca ttggcgaaaa agcaaaagaa
    10201 cacaaatata cagtgatgat gggtcggaca cacggtgtcc atgctgaacc aactactttt
    10261 ggattgaaac ttgccacatg gtattcagaa atgaaacgga acatcgaacg atttgaacat
    10321 gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag tgggaacgtt cgcaaatatc
    10381 ccgccattta ttgaagagta tgtatgtaaa caattgggga tccgtccaca agaaatctct
    10441 actcaagttc tccctcggga tctacatgct gaatatttct cagcgatggc gcttattgct
    10501 acaagtattg aacgttttgc aacagagata cgcggattgc aaaaatccga aacacgtgaa
    10561 gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg ctatgccgca caaaaggaat
    10621 ccgatcggtt cagaaaatat ggctggtcta gccagagtgg tgcgcggaca catggtcact
    10681 gcctatgaaa atgtcacatt atggcatgaa agagatattt ctcattcttc agcagaacga
    10741 atcatcttgc ctgataccac gattttattg aattacatgt taaatcgttt tggcaatatc
    10801 gttaaaaatc taacggtctt tcctgaaaac atgaagcgga atatgtctgc aacatttggt
    10861 ttgatttata gccagcgcgt tatgctgaaa ttgatcgata agggaatgac aagagaagaa
    10921 gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg acaaccaagt gcagttccgc
    10981 ccgctgttag aagaagatcc aaccatcaca tcggtattat ctaaagaaga aatagacgat
    11041 gcctttgatt atcatcatca tttgaaaaac gttgacgtga tattcaaacg agtaggtttg
    11101 gaataaagaa gagtgagttg caaaattcaa ttaaaataga atagcaaaaa taaccaaaaa
    11161 agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct tatcaataat
    11221 aggaacctga ttttaaaagc ggctacctct ttaccgtgcc tctttacata caaaaaatag
    11281 aggaattaag cgtaaatggc ttggctatta taactaaaat gctggttatg gagatgttgc
    11341 agcaagtttt cgagaaaagc gtattccatc tcttgtccat tgaataagat ctggatacga
    11401 taatgatttt tgaaacgctt ctgttcatga atctgactga ttgaaagttc gcctttgtgt
    11461 tctgtctgat aaagaaaagt ccttttacgg cattcagcac agcgcttggt tgctcggcgg
    11521 atttttcgga atgtataaaa actagtatct ttattgcata aataaacgtc tttgatcgac
    11581 agcaaatcat tatatagaca ggaacgttct tcagctgtta attctgataa aaaaagctga
    11641 ccttgtttta ttgcatcttc catcggcaag tgcctcctcg aacccattat ccaaattttt
    11701 agttaattct aatataccat cattaagtat aaatgtaaac gcttgttgat ggtataagat
    11761 tttcgtttct gataaaacac gaacattaaa agaatctaat gccttaaatg tacatatttt
    11821 tgttgattta taaaaagatg attgctaaaa tttaaagtga aaaaacgaac aaaaaacgat
    11881 gtatttgctt ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa gaacttttgt
    11941 atgaaggaaa agccaaaaaa atatatctta cttctgatga gaacttgatt tggatagaat
    12001 atttagatca agcaactgcg ttgaatggcg cgaggaaaga ccagataagt ggaaaaggag
    12061 aattgaataa tcaaatcaca agtgcaattt tccgttatct gactcaaaga ggaataaaaa
    12121 atcattttat tgaacaattg tctaaaaccg aacaattagc aaaaaaagta caaattatcc
    12181 ctctggaagt ggttatccga aatattgcag caggaagttt ctcaaaacgt ctaggtgttc
    12241 ccgaaggtaa agaattcatc aaaccaatca ttgaattcta ctataaggat gaccagttgg
    12301 atgatccatt tatcaatgaa gagcatatct atttcttaca acttgcaaaa ccggaagaaa
    12361 tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa ttatttgcta
    12421 aggtcggttt ggttttagta gatttcaagc tagaatttgg ccggtcgaat ggacaaattt
    12481 tgcttgcgga tgaagtaaca ccggatactt gccgattatg ggatgaacgt acgaatgaac
    12541 atatggacaa agacgtttac cgcagagatc taggtgattt gatcccagtc tatcaagaag
    12601 tatataaccg tctatcggaa ctaactgaag aaaaggagcg agtatgatgt atttcgtaaa
    12661 agtatatgta acctataaag attcagtgtt agatcctcaa ggggaggcag taaaaaaagc
    12721 agtccatcgt atgggatatg aaacaatcga agatatccga ataggaaaat actttgagat
    12781 acaacttgct gatgcagaag aaccaatcga tacagtgatt gaaacgattt gtgacaagct
    12841 gcttgcaaat gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg aggaagtata
    12901 acatgaaatt tgcggtcatc gtttttccag gttcaaattg tgacgcagat atgttatggg
    12961 cagttcgcga gatcatgggc gctgatgccg aatttgtacg gcatgatgag tcctctttag
    13021 aaggatttga tggggtttta ctgccgggag gattctccta tggtgactat ttgagatgcg
    13081 gtgcgattgc tcggttttct acaatcatgg aagaagtgat tcgttttgct gaagaaggga
    13141 aaccagtatt cggtacatgc aatggattcc agatattgac agaagcagga cttttgccag
    13201 gtgtactgag acgaaatgag tctttgcatt ttatttgcaa aactgtacca ttgaaagtca
    13261 accccaagac ggcctttact tcagagtatg aagccgacga aataatctat cttccagttg
    13321 ctcatgggga aggaaattat ttctgtgacg aagaaacact tcaagaacta aaaaaaaata
    13381 atcagattgc ctttacttat aatgacaaca tcaacggcag cgtggaaaac attgcaggga
    13441 ttacgaacaa agcagggaat gtgttaggaa tgatgcctca tccagaacgt gcagtagaag
    13501 acttgctagg atcagaagat gggaaacggt tctttgcttc cattttaaaa aacttcggaa
    13561 aggtgaccgt atagatgaaa acattgaaag aaccgacagc tgaagagatc aagaaaaaaa
    13621 gaatctatgc cgaatgggga ttgactgatg aagaatatcg aatgatcgaa gaagatatct
    13681 taggaagaat gcctaattat acggaaactg gactgttttc tgttatgtgg agcgaacatt
    13741 gttcctataa aaattctaaa ccggttctac gcaaatttcc tactagtgga cctcatgtgt
    13801 tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg tgatggacta gcagttgtct
    13861 tcaaagcaga aagtcacaat catccttcag ccgtggagcc ttatgaagga gctgcgacag
    13921 gagttggcgg aatcatcaga gatattttca gcatgggggc tcgtccaatt gcgttgctag
    13981 attcattgag gttcggcgaa ttagataatc caagaacgaa atatttacta gaagaagtag
    14041 ttgcaggtat cggcggatac ggcaattgta tcggcatacc gactgtaggc ggcgaaattg
    14101 catttgatcc ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta ggtttgattg
    14161 aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg taattcgatc atgtatgtag
    14221 gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa gaatttaatc
    14281 aagaagaaga acagcaacgt tctgctgtac aagtaggcga tccttttatg gaaaagctgc
    14341 tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga ttctctaata ggtatacagg
    14401 atatgggagc agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa gctggctcag
    14461 gacttatatt gacattagat gaagtcccac aaagagaaac tgggatgaca ccttatgaaa
    14521 tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt taaatcagga gaggaagaaa
    14581 aaatccaaga actatttcag aagtatgatc tagatgcagt cactatcggt aaagtaacag
    14641 acgacgggca atatcggcta tatcatcaag gagaagaagt agcaaatctg ccagtcgatg
    14701 cattagctga agatgcacca gtttatcata aagaaatgaa agaaccagct cgaatcgtag
    14761 aatttcagca gttagctcct tatcagccag ttatcgaaga acctggacaa gtattgcttg
    14821 atctgctgca gcaaccgacg attgcttcaa aacgttcaat ctacgaaact tatgattcac
    14881 aagtacaaac caatacagtt gtccgtccag gaagcgatgc tgctgtgatg agggttagag
    14941 ggacgaataa agcgttagct atgaccacag actgcaacgc gcgctatctg tatttggatc
    15001 caaaaatcgg aggacaaata gcagtggcag aagctgctag aaatattatt gctagcggtg
    15061 gtaagccctt ggcgatcacc gactgcctga actatggttc tcctgataaa ccagaagttt
    15121 tttgggaatt atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg ttaggaactc
    15181 ctgttatttc aggaaatgtc tctttatata atgaaacgga tggtcaagca atctatccaa
    15241 caccaatgat tggtatggtc ggattgattg aggatcataa acatatcact actcaggaat
    15301 tcaaaaaatc aggggatcta atctatatat taggtaagac gtttgcagat tttgatggca
    15361 gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac tttgatctat
    15421 ccattgaaaa gagaaaccaa gagttagtat tgacagccat ccaaaacgga ttgattgaaa
    15481 gtgctcatga ctgttcagaa ggtggcttgg caattgcact ggcagaatca gctttcaaac
    15541 atcagctagg gatatctgta cagtttgaat tgtcctctgc tcaattattt gcagaaactc
    15601 aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa gaaatgatgg
    15661 gagatgctgc tgttttagca ggtaaggtga cggatgaagc gataatcgaa atttcagcaa
    15721 ctgacggtca gatcaaaata gaaactgcag tagcaagaaa gtgctgggag gatgcaatcg
    15781 tatgtcttat gaagtgaaga gtttaaatga agaatgcggg atcttcggtg tttggggtca
    15841 tccagacgcc gctagtgtgg catacttcgg gctccatagt cttcagcatc gaggacaaga
    15901 aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag gggcatcgag atttggggct
    15961 gctttctgag gtattcaaag atcaaagaaa attgcagcag ttaacaggag aagctgcaat
    16021 cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc agccttttct
    16081 attcaaattt catgatcagt cgatcggctt agcgcataat ggtaacttga tcaatgctcg
    16141 ttcccttaga aaagagttag aggaaaatgg ggcgatcttc cattctaact ccgatacgga
    16201 gatattgatg catttgatcc gacgaagtga agaaacagat tttttaaata aattaaaaag
    16261 tgctttgaat gaagtcaaag gtggctttgc ctatttatta atgacagaaa cggcaatgat
    16321 tgccgctttg gacccaaatg gttttcgtcc attagcaatc ggacaaatga aaaacggagc
    16381 atacgtgatt gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt ttataagaga
    16441 tgtccagcct ggtgagatag tgatcatcaa tgacgaagga atccagattg attgctttac
    16501 gaaggacacg cagctttcta tttgttcgat ggaatatatc tattttgcca gaccagattc
    16561 aaacatcgca gggatcaatg tgcatactgc acggaaaaat atgggacgga atttagcgaa
    16621 agaatcgcca gtaaaagcag atatggtcgt tggcgtacct aattcttctt tgtcagccgc
    16681 aagcggttat gcggaagcta gtggaatccc ttatgaaatc ggattagtaa aaaatcaata
    16741 cattgcccga acatttatcc agccaacgcc tgagctaaga gagcaaggtg ttcgaatgaa
    16801 attatctgct gtacgaggag tagtggaagg taaaaaggtg attatggtcg atgattcaat
    16861 tgttcggggt acgacaagca gacggatcgt ccatcttttg aaagaagcag gggcaaaaga
    16921 agtccatgtg aggattgctt ctcctccttt gaaatatcca tgtttttacg ggattgatat
    16981 tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt gatgaaatca aagaatgtat
    17041 cggtgcagat tctttaggtt ttttaagtga agagggtctg atcgaatcga tcggtctgga
    17101 aaaagatgct ccgtattcag gattgtgtat ggcttatttc aacggagatt atccgacgcc
    17161 gttatatgac tatgaagaaa aatatcagga atcactaaaa gaaaaagtat cgttctttta
    17221 aaagcagacc aagaagggag tatatgaatt caatggcaaa tgcatacgca aaagctggcg
    17281 tagatgtcga agctggatat gaagtagtag aacggatcaa gaagcatgtc aaaaaaacgg
    17341 aacgtttagg tatgatgggc gctataggag gattcggcgg ttgttttgat ttatcggtct
    17401 ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg cgtggggacg aaattagcag
    17461 tggcaatcga agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg atgtgtgtca
    17521 atgatatctt agcacaaggc gcagagccgt tatatttctt agactactta gcaacgggga
    17581 aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt agcagatgga tgtgttcaag
    17641 gaaatatggc gctgattggc ggagaaacag ctgaaatgcc tggtatgtac tcaggtaatg
    17701 attatgatct agccggattt gctgtaggga tagctgaaaa atctcaatta ttgacaggag
    17761 aaacgatcaa cccaggagat gtgttgcttg gaatagcatc tagcgggatt cattcaaatg
    17821 ggtactctct tgttcgcaaa attttctttg agcagcagca gttttcttta tcatcaaaac
    17881 tgccagaatt gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa atctatgttt
    17941 cttccttgct tccgctgatc aaagaaaaga aaataaaagg agctgcacat atcactggtg
    18001 gagggtttgt agaaaatatc ccgcgaatgt taccagaatc tatggctgca caaatcaaac
    18061 ttggctcatg gccgattctt ccaatattcg gctgtttaca aaaatatggt gaaattcctg
    18121 agatggagat gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca gtcgaagaat
    18181 cgaaagtaaa agaaatagta cacgaaattg agcaagctgg cgaaaaagtt tatgaaatcg
    18241 ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa cgatgagaat
    18301 cgctgttttt gcttctggaa acggaagcaa ctttcaagcg ctagccgatt atctatcgaa
    18361 aaaaggattg gaatcttcga ttgactggtt gttttgtgat cagcctgaag catatgtttt
    18421 gaaacgtgca actgcgttga gtgtaccggc agattgtttt tcacctaaag aatttgattc
    18481 aaaaaaagag tatgaagaag caattttgca taaattgaaa gaaaaaaaga tcgatttgat
    18541 cgtattagct ggatatatgc gaatcattgg accggtattg ctgaaaaatt atgataaaag
    18601 aatcatcaat atccatccgt ctcttttacc tgccttccct ggtttgcatg gcatacgtga
    18661 tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt cattatatcg accaaggtgt
    18721 agatactgga ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg acacgttcga
    18781 ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc tatccagaag tgattagtga
    18841 aattattgaa aatggaggct atctgaaatg acaagagcat tgatcagtgt atcagataaa
    18901 actgggattg tagcattcgc ccaaggtttg attgaacaag gagtagaaat catttcaaca
    18961 ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa cgctttcgat cgaagaagtg
    19021 acagggtttc ctgaaatgat ggatggccgt gtcaaaacac ttcatcctaa aatccatggt
    19081 ggtcttttag gaagacgcga tctccctagc catatggagg caatggaaac ggaaaatatc
    19141 caaccaatcg actttgtttg tgtcaattta tatcctttca aagaaacgat tatgaaacca
    19201 gatgtcgaaa tagcagaagc tattgaaaac attgatatcg gtggtcctag tatgctccgt
    19261 agtgcagcaa aaaaccatga atttgtcacc tctgttgttg atccatctga ctatgaagaa
    19321 gtactttcag aattacgtca gtatgggcat acttcacttt ctttcagaca aaaactagcg
    19381 gcaaaagtct ttcgtcatac tgcggcatat gatgcactga tcgctcaata cttaacggat
    19441 tgggtggaag aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa acaaacgctt
    19501 cgttacggtg aaaatagcca tcaagccgct gccttttatc agtccgctct tctggaaaaa
    19561 tattcgatcg cagcagcaaa acaactccat gggaaagaac tttcttataa taatatccga
    19621 gatgcagatg ctgcgctacg gatcatgaag gaatatgacg agccaacggt agtcgccttg
    19681 aaacacatga atccttgtgg tattgggaca gcatctacga ttttagaagc gtgggaagca
    19741 gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa tcgagaagta
    19801 gatctgccaa cagcagaaca gatgcataag ctgtttctag aaatcatcat tgccccttct
    19861 tatacacaag aagctttgga attactgcaa aaaaagaaaa acatccgatt actgacagta
    19921 gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt aggtggactt
    19981 cttgtacaag atcaagatct ggcaatagaa aatgaagaag agtggaaagt cgtgacaaaa
    20041 cgtaagccga ctccagcaga agcaaaagca ttagcgtttg cttggagagc tgtcaaacat
    20101 gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga cagtaggaat cggagccggg
    20161 cagatgaatc gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga gaaaatggaa
    20221 ggtgcagttt tagctagcga tgcttatttt ccaatggatg attcggttga atatgcagcc
    20281 aaacatggta taaaagcaat cattcagcca ggaggaagca tcaaagacca agattctatt
    20341 gatatggcga ataaatacga tctagcaatg gtctttactg atgttcggca ttttagacat
    20401 tagagaggag tcggataaat gaacattttg gtgatcggtg caggtggtcg tgaacatgca
    20461 ataagtaaaa aacttttagc cagtccgaag gtggatacgg tgtattgtgc gccaggaaat
    20521 ccggggatga caaaagatgg tatcaattgt gtcgctatca gtgagcgaga tcattcagcg
    20581 ttaatccatt ttgttaagaa agaggctatt gattggagta tcgttggtcc tgaagttcct
    20641 ttgttaaatg ggatcgtcga tgattttatg gcggaaggat tgaaaatctt cggtccgaca
    20701 aaagcagcag ctctgatcga aggttcgaaa agttttgcta aacaattaat ggatacttat
    20761 caaatcccga cagctaaatc cagaagtttt gaatgttacg aaaaagcagc agcgtatatc
    20821 aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg gaaaggtgta
    20881 gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt gaaccagcat
    20941 cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg agaagaattc
    21001 tcccttttgg cgtttgtccg taataccaag gtgtatccga tggtcatttc gcaagatcat
    21061 aagcgggcat ttgataacga taaagggcca aatacaggcg gaatgggtgc ttattcacca
    21121 gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt aaaaccagcg
    21181 gctttgggaa tgaaagaact aggacgctct ttcacgggtg ttttatatgc gggtttgatt
    21241 gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga tcctgaaacg
    21301 caagtcgttc tttctcggtt gaagagtgac tttgcgcaag taatcgatga tttattagag
    21361 aatcgtacag tagagttaaa gtggcagcaa gaaggataca atgtaggcgt tgtggttgca
    21421 gctgccggtt accccgaaga atatgagaca ggaatggttc taccagattt tctggaagaa
    21481 gagctttctg tttactatgc tggagtttcc tcaaataacg gcaaactagt tggagcagga
    21541 ggacgtcttt atttagtaga agcatacggt gagacgatca aagaagcaca gaaaaaaatt
    21601 tacaccgcgt taatggataa aacgacagat ggaacatttt atcgtacaga tattggagaa
    21661 aaagctgcca atagcgaact gatcgtgaag taaatcagct gcttccattt ataaaaaata
    21721 caacaaacat aatcggaagc gccgatttta gtgaagcctt ttcataaaga gtgtggtaca
    21781 cttttgttgt gtatataaat aaggaggagt tcagatgaaa aaaattcaat taggtacttc
    21841 tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg cggaagatcc
    21901 agtaaaagtg attgagacag cttatgaaaa cggcattgat ttttttgacc atgcagatat
    21961 ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa catcaatcaa
    22021 acgagaagat ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga tgtttgattt
    22081 ttctaaaaaa catatcattg aatctgtaga aaaaagtttg acccgtctga aaatggatta
    22141 tgtggatgct ttacttcttc atcgtccaga tacattggta gaaccagagg aagtagctga
    22201 agcgtttgat gaattggaac ggtcaggaaa agttcgttat tttggcgtca gcaatcaaaa
    22261 accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag ctaatcagct
    22321 gcaatttggc ttgaaacata ctggaatgat cgatcaaggg attcatgtca atatgacaga
    22381 tgaagcaagt atcgatcatg acggtagtgt acttgattat tcccgattgc atgatatgac
    22441 gatccaagca tggtcaccat accagtatgg attttttgaa ggtgtattta tcgggaacga
    22501 aaaattccct gaattaaacc aaacattaga agaactagca gctaaatatc aaacgacacc
    22561 aactggcttg gcttctgctt ggattttacg ccatccagcc aatatgcaag tgatcgccgg
    22621 tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta atatgtctcg
    22681 ggaagattgg tacgaattgt accgtgctgc tggaaacatt ttaccttaaa gattgtcata
    22741 agcaggtcaa aagggctata aattaaaaag acagaataac ttgaaagctg ttttcagctt
    22801 cgagatattc tgtctttttt tattgcaaat ggaaagagta tcattgtacg gatttcgttt
    22861 attagttatt tttgttttta aggaaaagct gtgtccgata ttcttttgga gaaatccctt
    22921 gttttttctt aaataaataa ctgaaatagt tagggtcatt ataaccaatt tcaaaagcaa
    22981 tatctgaaat acgatagtct gattgctgca ataatttttt tgctaattcg attctttgtt
    23041 ctgtcagata atcgatgaat gtttggttaa acgattgaga aaaaatcgta ctgaaatgag
    23101 agggacttaa tgcaacttgt tgagcgaccc aagttaagga catatctgga tcggtaaaat
    23161 tttctttgat aaaatccaat gcatggttca aaattggctg atatttgttc cttgaaggct
    23221 gtgagggcag agcagttaag aaagaaatga acatataaac tgtcgaacgg taaagcgagt
    23281 cgtcggcgct gattgctaag cgttgactgg tggatgtgca atgaaccatt tcttgcggga
    23341 ttttcttttc tttcaggcag acatgctgga gtagcttagt caagacaaat aaacgatata
    23401 atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac ttagatactt
    23461 cgtttttcga taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca gaatcaactg
    23521 gaagtttcgc ttcttgaatc aaatcctcat agctgataat ttgttcttta agggaactgg
    23581 cggtttcaag tatcatctgc gtgatctcaa aagatgcagg taattcgctg attctttctg
    23641 cgacaggacc aatggcgaca gaaaaattat ttttttgtcc cttttctaat tcatggacca
    23701 acgtttgggc tacctgataa ctggtctcga tcacattttc ttcaagagaa tcaactatta
    23761 aaaatttgat gaatcgagag gaaacagcag agaagatcac ttgttgattc tccctaaata
    23821 aaaacttcaa ataatcactg agatgagcgt aatcttcaaa gtctgtactg acttggtttg
    23881 ctgctaataa tacggtcatt tttttccctg ctaaagagcg attgaaagat tcagcaagtt
    23941 tcaatgtttc actgatcgta agtgagcctt tgaacaaacc gttcaagaaa tgattttttt
    24001 tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa tctaaagcag
    24061 ttactgcttt ttctattgct tgtttcaatt cgctactttt aatcggttta agcagataat
    24121 catctgcctg tacctggatt gctcctttca tataatcgaa atcgtcgaat ccactaataa
    24181 atatgagacg agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc aacccgtcca
    24241 tgaaaggcat ttggatatca gtcagaatga tgtcgggctg taagtcgata attgaagaca
    24301 gtgccatctc accatctcca gcttcgccga cataagtgat tggataataa gaaccaagga
    24361 cagttatttg ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg ataaaaactt
    24421 tatacatgtt tgtttcctcc aattgttttg ggagatagag ggatcatcag tgtgacagtt
    24481 gttccttctc ggtatttgct gtcaatcgtg ataggttttt gatcatttcc gtaataaaga
    24541 aggagacgct tgtttacgtt gtaaagtccg tagccgatac tgaaatctga atcgatccct
    24601 ttggctaact ctttacgtat ttctgccagt ttttctgcag ggatgccaat cccgttgtct
    24661 ataactttga attggatcat ctcatcaatt ttgcatgcgg taatacgaat aaatcctttg
    24721 tgacggacgt acttcgttcc atggtataca gcattttcaa tcaatggctg caagatcatt
    24781 tttaaaatag aaacaggaaa aagtgtttct ggtatatcga tcttatagtc aagcatatgg
    24841 ccgtatctta tttttaagat gactagataa tcattgatat ggctaatttc tttttcaaga
    24901 ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa agccggaaag tgcatgggtc
    24961 atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc atccaaactg
    25021 ttataaataa aatggggagt aatctgagct tgcaatacac gaacttcact ttgtgctaaa
    25081 tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc cattttattc
    25141 atgctagcgg taagtgtttg taattccagt gtttccggca gggttaaacg aaagccaagg
    25201 ttccctgaag aaagttgttc tgacatctcg atcagctgat tgatcggttc ttggaccttt
    25261 ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa tggtactaag aactccaagt
    25321 tccactattg ttaatagaat gagcgagcgg acaagtgtcg tatttttttt gcttgctaag
    25381 tcgatctcca cacgaacaaa atcttgtaag atatctgaca gcagttgagt gacagaatct
    25441 acctgattga tgatctcttc attggtatcc ataaaatctt ttgccaagat attattgatc
    25501 agctcgtttt gataatcttc cagtgtatcg atcgtacgta gcgatacatc taacgtacta
    25561 atttctttaa gtgtatttgt attatttttg atctctcgaa tatcattcct aagttcttct
    25621 aggatatttt cctcattgaa ctcatctacg gttacttggc cagaaatcaa gtcccacata
    25681 ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat ctgctttttg gatatttgcg
    25741 atcgtctgtt gataaatcaa aagctgttgc gaatataaca gtatgctcag tagcaaaggg
    25801 ataacagcaa atagaagcat ggctttgttt gcttgggcaa aaatctgttt tatgcttaaa
    25861 ttttttgtca tgatacttct ccagtcaata atttctggtt gggatgttag ctatattctg
    25921 ctgggaaaag acagtctcgg aaataaaaat atctttaggt aatgtttttc ctgtttgaat
    25981 atttccaaaa taacgagtaa cggtattggc tacataccag ccgacatcag gtttacactc
    26041 gactacacaa ttgacgattc ctgagcgtag cttctcgatc atatcttctt gtccatcaat
    26101 agttacaatg atcaaatctt ctccaggttt gattgatgtt tcttttatcg cagctaaagc
    26161 accgtgagtc atttcatcac tatggctgta taatacatcg atttcggata aatcatttgt
    26221 ttctaaatat cgtttcatct ggtcttttcc tttcatccga ataaaatcac cgacaattcg
    26281 gtgaacaata gtcatcggag gtttatggtc gatggttcca tcttttttta gggttggttt
    26341 acgattgatt gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg agttctctga
    26401 accagcaagt tctaggattc tgatttcctg tttctttgaa tttgtgaagt gattgctgac
    26461 aaataaaccc gcccgatttc cttctgcttt gaaactgggg ccaatgtggg tgacgaaaag
    26521 ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc ctgctcgctt
    26581 tgcttctttt aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa tgatcatatc
    26641 cactttatag gcaatgaacg tccgtatatc ttggatttgc cgttcctgat tcatgtagcc
    26701 atttcggtag agaacttggt aatcttcttt ttcaagcgca gtgataattg attcagtatg
    26761 ctttttccgc cagctactct ccgttcctga ttgcgaaaaa ccaacgacta gtgggtcagg
    26821 tgcttccttt gtattaggtg tacaccccat catttggtaa gcgattacaa caagggctaa
    26881 aaaaaataaa acacgtttca ctttaaatca ctccatctgg atttgttagt actatcttat
    26941 cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga aaaaaatcca agaaacttaa
    27001 aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa gtaaatccca taaaaaaatc
    27061 aatgttaacg ctttctttat tagctatact cggtgtatca attgaaatgg gggataaaaa
    27121 atgaaaaaca aatttagaaa gattgttggg actatcgcta ttttgaccgc aagcattcta
    27181 ttaagtgcgt gtggcaattc aggatctgca gatgatagta cggggtatgt aggaatcgct
    27241 atgccgacca aatcagcaga aagatggatc gctgatggga acaacatggt ctcggaatta
    27301 gaaaaactgg gttataaaac agatcttcaa tatggagaag ataaagtaga aaatcaagta
    27361 gcacaaatag aaaatatgat tacaaaaggg gtagatacac ttgtgattgc ttctatcgat
    27421 ggttcggctt taactgatgt gttagctaaa gcaaaagaag cagatatcaa ggtgatcgcc
    27481 tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt acgcgacttt tgataatttt
    27541 ggagtaggtg tatcgcaagc agcttatatt gaagagcact taggattgaa agaaggaaaa
    27601 ggaccattca cgattgaatt atttggtggc tcgcctgacg acaacaacgc actaatcaat
    27661 tacaacggag tgatgtctgt tttacaacca tatatggaca acggtcagtt ggtcgtgcca
    27721 tctggacaga cgagtttcag tcaaatcgct acgcttcgct gggatggttc tacggcgcaa
    27781 gcaaggatgg ataatctgtt aagcgcaaat tatactgacc aaacattgga tgctgttttg
    27841 tcgccatatg atccaataag tttaggaatc atttcgtcat taaaaggcgt ggggtatggg
    27901 agtgaaagta aacctttacc tgtgattact ggacaagatg cgacagttgc tggcgttaaa
    27961 tcgatcattg ccggggaaca aacacaaacg atctttaaag acacacgtat cttagcaaaa
    28021 aatacgattg aaatgatcaa agcaatcagt gatggagaag aggttccggt caatgatact
    28081 gaaacttatg ataatggggt gaaaactgtc cctacgtatt tagcaaatac agtatctgtc
    28141 gataaagata attatcaagc agagctgatc gacaccgatt attataaaga atccgatctg
    28201 aaaaattaag aggaaccaaa caaggaacta aagagatcta agtcagagag aggataagaa
    28261 ttatggcgga ttgtatttta aaaatggcag atatcaccaa aaaattttct ggaatccgag
    28321 cgttagattg cgtgaatctg atgattgaac gtggggaaat ccatgcattg tgcggagaaa
    28381 acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg cttgtatcca tatggctcct
    28441 atgacgggga aatcttttat gatggaaagc tgtgtcaatt taaagatttg aaagatagtg
    28501 aagaaaaagg catcgtcatc attcatcaag aattagcact aagtccttat ctttcaataa
    28561 aagagaatat ttttctcgga aatgaacaag cagaaagagg cgtgattgat tgggaactga
    28621 cagaaaagaa aacatatgaa ctgctgaata aagtcggttt gtctatcgac ccgaatcttc
    28681 tagtatccca gattggcgta gggcaacagc aactagttga aatagctaaa gcattatcaa
    28741 aatccgtccg tctgttgatt ttggatgagc ctaccgctgc attgaatgaa gaagaaagcg
    28801 cgaatttgct gagattgatc cgagagttga aagagcaagg tgtaacctcc atcattattt
    28861 cacataagct gaatgaaatc gtggatatcg cagatcgaat caccatttta cgggatggac
    28921 agacgattga gacactagaa aatgaaatga tcagcgaaga gcatattatt cgcgggatgg
    28981 tgggcagaga tttagttgat cgttatcctg aaagaaaacc ggatattgga gaagtttatt
    29041 ttaaagtaga agactggacg gtctatcacc ctcttgatca cgagcgaatc ataaacgacc
    29101 acatcaactt gcatttgaaa aaaggagaga ttgtaggaat agctggactg atgggagcgg
    29161 gcagaactga attcgctatg agtctatttg gacattctta tggaagcaag atatccggac
    29221 gtatattaaa ggaaggggca gagatatcag ttaagaccgt tccttcagcc attgcaaacg
    29281 gactcgctta tgtctcggag gaccgaaaat ctttaggact gaatctgctg atggatattc
    29341 gggaaaacgt gacgctgagc agtttggata aaataagtca aaaaggagta ttagataaag
    29401 aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat gcgtataaaa acaaactcca
    29461 tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca aaaagtcgtt ttatccaagt
    29521 ggctgatgac acaacccgat attctttttt tggatgagcc aacccgtggg atagatgttg
    29581 gggcaaaata tgagatctac acgattatcg aagaaatggc agcaaatggc aaagctgtct
    29641 gtcttatctc ttctgaactt cctgaaatat tagggatgtg tgatcgaatc tatacgatga
    29701 acgaaggacg tattacagga gaagtctcgc gagaagatgc agatcaggaa gtcttgatga
    29761 aattaatgac gaaagaagag gtgggttaag ttggaacagg caaaaaatct tacggataaa
    29821 aaagagaaaa aactaaattt gaaaagtaga gcagctgatc tttttggtcg ctacagcatg
    29881 gtgatcattt tagcgggact acttattgcg tttcaattga tgacggatgg tattttctgg
    29941 cgtccattga atataactaa ccttgttctg caaaatagcc atatattagt actagctgca
    30001 gggatgttat tagttgtgct cttaggtcat gtggatttgt cggtagggtc agtgatggcg
    30061 tttgttggtg caattgcggg catgatgatg gtcaatcgcc aaatgaaccc gtggatgaca
    30121 gttatcgcat gtcttgcagt aggcgggctg atcggcgcat ggcaaggttt ttgggtagct
    30181 tatgcaggga taccggcgtt tatcgtgact ttagcaggat tgctgatgtt tcgtggactg
    30241 acacaagttg ttctaggagg ccaatctcta gcaccttttc cagctgtttt ccaaaaaatg
    30301 tctacgggtt atttaccaga cttaagcgaa ggaagcagcc ttcacttctt gacattgatt
    30361 ttaggaatga tcatcacgat cgtgttagtt gttggtcaat ggcgaacaag aaatcgtcgg
    30421 aaaaatcatt tatttgaagt ggaatctcca acagctttct tggtaaaggc ggtatttaca
    30481 gcagtcgttg tactaggaat aacttatatc tttgcctctt atcaagggta tccgattatt
    30541 ttgatcattc ttggtttgat cgttgcagca tatgggtttt tgacaaataa aacagtagca
    30601 ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg ctgaactatc agggatcaaa
    30661 acaaagcgaa tcacgttctg ggtattcgtc aacatgggag tcatggcagc tttagctgga
    30721 ttagttttag ctgctcgatt gaatgcggca acacctcaag caggtacatc gatggaactg
    30781 gatgcaatgg cggcagtata ttttggtggt gcttcgactt ccggcggcat tggaacaatc
    30841 atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga acaatggcat gtcgatttta
    30901 ggcgtcgggg tagactggca gcaggcaatc aaaggattga tcttgctttt agccgttgtg
    30961 ctagacttgt ataataagaa aaaacgaaca acgtaggaga acgccggtta ttcagaaaaa
    31021 agactgtgac aagacatttg tcacagtctt tttttcgcta aaagcgaaca ttcattgttt
    31081 cgtttgtata gactaaaaat aatatatgtt tttttaccat tcgtataaat tctctttttg
    31141 ctcttgattt ttttgcttaa ttgagttaaa ttacataggg cgctaaaaaa agccttgatt
    31201 aaattaataa tgttcgtgtt ttgctggatg tgaacatcag gaggataatg taatgaaagt
    31261 atttgattac gaagatattc aattaattcc taataaatgt atcgtaaata gccgttctga
    31321 atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag tgcctgccaa
    31381 catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg gttatttcta
    31441 tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa tgaaaaagcg
    31501 tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct ttatagagaa
    31561 gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc atggacatgc
    31621 taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa cttttgtgat
    31681 tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg gcgcagatgc
    31741 aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga caggctttgg
    31801 gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc gcaagccgat
    31861 catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc gttttggtgc
    31921 gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg gcgaaacaaa
    31981 agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat tccaaaaagg
    32041 cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat ctttgaaaga
    32101 tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg gaggaagaga
    32161 tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt ttaatggaga
    32221 cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc tgcattaagt
    32281 aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg aattattatc
    32341 gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa gatttttgtc
    32401 acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc ggtaaaatca
    32461 aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga gaaaaaacaa
    32521 ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa cggaaggatc
    32581 aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg tgtgaatcgt
    32641 tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac gagcaaacgt
    32701 ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc aagaatcgat
    32761 tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca aaaaataaga
    32821 gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga gatgccagat
    32881 gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt agcggaagaa
    32941 aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg gtatttggaa
    33001 agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg cggtcagcaa
    33061 gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc tgtagagtca
    33121 ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt atttgggata
    33181 ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca taaaaataaa
    33241 ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt gatgagtcaa
    33301 aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat tattgaagac
    33361 gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat acgaacgatt
    33421 gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa aacaacgaaa
    33481 atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc gcaagaaatg
    33541 atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt taatgaagca
    33601 attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg actgataagc
    33661 gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg ttactatgcc
    33721 tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt agtagatatg
    33781 ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat cagaatcaat
    33841 tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa aattctagaa
    33901 aagtggttaa cagaataatt tcacaaagta aatagattaa agaatataac agcttggatt
    33961 taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga aactaagaaa
    34021 aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt gcaacaaaac
    34081 tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt agaaaataga
    34141 aaaaatgcag atgaaatata aaacgctttc cttattgtga ttttattcac taatgctgtt
    34201 aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta gcttgtattt
    34261 ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca gttttattca
    34321 ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg tgtgacaaat
    34381 ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca atgcagattt
    34441 cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt tagtacctga
    34501 tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc gtgtattaga
    34561 ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac caactgctgg
    34621 acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg ttcttcttcc
    34681 aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc aagctttttt
    34741 atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag ctctaccacc
    34801 acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag gtttgaagaa
    34861 acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat cacaaggtga
    34921 tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat tttatatcca
    34981 aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa aaacacttgc
    35041 acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg atccgcttgc
    35101 agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg gaccagtcct
    35161 aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg atccaacccg
    35221 ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga tccgcttccg
    35281 taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag tcatcgaagc
    35341 aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa aacaaaaggt
    35401 ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag aacaaattgc
    35461 aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat ccaagcaatc
    35521 acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt tggtgaagat
    35581 gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa gttcggagaa
    35641 gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag tttcggtctt
    35701 gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat tttcgaagca
    35761 atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg cactagaaat
    35821 ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga attgcattcg
    35881 gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat tccttccaac
    35941 ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc ggttgttttc
    36001 ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga agcttacgaa
    36061 gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat tatcacatac
    36121 ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga aaatatatct
    36181 gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat cattcaatcc
    36241 gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc gggcgttgcc
    36301 gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc cccaatcgga
    36361 cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt ctggttgcca
    36421 aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga aaagaaggga
    36481 cgaaacaaag tcatggctta tcaatttaaa ttgccggata tcggtgaagg aatcgcagaa
    36541 ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga tgatacatta
    36601 ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt cacctgttac aggaacggtt
    36661 aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg gggatgtatt agtagaaatc
    36721 gatgcaccag gacacgaaga caatgaaggg gacagtggtg tagccgcaga atcccaaact
    36781 ccagctaaac cggctgctga acccactgtt gatacagaat cagctggatc gtcaagcgaa
    36841 ggagtcttcc aatttaagtt
//
LOCUS       DAHHPA010000016        35774 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.017, whole genome shotgun sequence.
ACCESSION   DAHHPA010000016 DAHHPA010000000
VERSION     DAHHPA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.017"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            108..1178
                     /locus_tag="NBH55_000748"
     CDS             108..1178
                     /locus_tag="NBH55_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508753.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1193..1861
                     /locus_tag="NBH55_000749"
     CDS             1193..1861
                     /locus_tag="NBH55_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1508754.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1881..2459
                     /locus_tag="NBH55_000750"
     CDS             1881..2459
                     /locus_tag="NBH55_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1508755.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2860..3771
                     /locus_tag="NBH55_000751"
     CDS             2860..3771
                     /locus_tag="NBH55_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD1508756.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4149..5066)
                     /locus_tag="NBH55_000752"
     CDS             complement(4149..5066)
                     /locus_tag="NBH55_000752"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCD1508757.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5268..5705
                     /gene="zapA"
                     /locus_tag="NBH55_000753"
     CDS             5268..5705
                     /gene="zapA"
                     /locus_tag="NBH55_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCD1508758.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5763..6311
                     /locus_tag="NBH55_000754"
     CDS             5763..6311
                     /locus_tag="NBH55_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCD1508759.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6426..8786
                     /locus_tag="NBH55_000755"
     CDS             6426..8786
                     /locus_tag="NBH55_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCD1508760.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8873..9187
                     /gene="trxA"
                     /locus_tag="NBH55_000756"
     CDS             8873..9187
                     /gene="trxA"
                     /locus_tag="NBH55_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCD1508761.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9369..11147
                     /gene="uvrC"
                     /locus_tag="NBH55_000757"
     CDS             9369..11147
                     /gene="uvrC"
                     /locus_tag="NBH55_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCD1508762.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11292..12191
                     /locus_tag="NBH55_000758"
     CDS             11292..12191
                     /locus_tag="NBH55_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1508763.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12184..13446
                     /locus_tag="NBH55_000759"
     CDS             12184..13446
                     /locus_tag="NBH55_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508764.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13607..15505
                     /locus_tag="NBH55_000760"
     CDS             13607..15505
                     /locus_tag="NBH55_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCD1508765.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15835..16554)
                     /locus_tag="NBH55_000761"
     CDS             complement(15835..16554)
                     /locus_tag="NBH55_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCD1508766.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            16891..17223
                     /locus_tag="NBH55_000762"
     CDS             16891..17223
                     /locus_tag="NBH55_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508767.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17210..19213
                     /locus_tag="NBH55_000763"
     CDS             17210..19213
                     /locus_tag="NBH55_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCD1508768.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19232..19702
                     /locus_tag="NBH55_000764"
     CDS             19232..19702
                     /locus_tag="NBH55_000764"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCD1508769.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19723..20307
                     /locus_tag="NBH55_000765"
     CDS             19723..20307
                     /locus_tag="NBH55_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCD1508770.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20322..21308
                     /locus_tag="NBH55_000766"
     CDS             20322..21308
                     /locus_tag="NBH55_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCD1508771.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21298..21609
                     /locus_tag="NBH55_000767"
     CDS             21298..21609
                     /locus_tag="NBH55_000767"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCD1508772.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21631..23412
                     /locus_tag="NBH55_000768"
     CDS             21631..23412
                     /locus_tag="NBH55_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCD1508773.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23405..24781
                     /locus_tag="NBH55_000769"
     CDS             23405..24781
                     /locus_tag="NBH55_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCD1508774.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24788..25423
                     /locus_tag="NBH55_000770"
     CDS             24788..25423
                     /locus_tag="NBH55_000770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCD1508775.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25453..26808
                     /locus_tag="NBH55_000771"
     CDS             25453..26808
                     /locus_tag="NBH55_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCD1508776.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(27000..28394)
                     /locus_tag="NBH55_000772"
     CDS             complement(27000..28394)
                     /locus_tag="NBH55_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCD1508777.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRSNQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28486..29457
                     /locus_tag="NBH55_000773"
     CDS             28486..29457
                     /locus_tag="NBH55_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCD1508778.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29454..30236
                     /locus_tag="NBH55_000774"
     CDS             29454..30236
                     /locus_tag="NBH55_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCD1508779.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30405..30776)
                     /locus_tag="NBH55_000775"
     CDS             complement(30405..30776)
                     /locus_tag="NBH55_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1508780.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30910..31353
                     /locus_tag="NBH55_000776"
     CDS             30910..31353
                     /locus_tag="NBH55_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCD1508781.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31396..32205)
                     /gene="yidA"
                     /locus_tag="NBH55_000777"
     CDS             complement(31396..32205)
                     /gene="yidA"
                     /locus_tag="NBH55_000777"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCD1508782.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32224..33591)
                     /locus_tag="NBH55_000778"
     CDS             complement(32224..33591)
                     /locus_tag="NBH55_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1508783.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33575..34405)
                     /locus_tag="NBH55_000779"
     CDS             complement(33575..34405)
                     /locus_tag="NBH55_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCD1508784.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34568..35005
                     /locus_tag="NBH55_000780"
     CDS             34568..35005
                     /locus_tag="NBH55_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCD1508785.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            35081..35701
                     /locus_tag="NBH55_000781"
     CDS             35081..35701
                     /locus_tag="NBH55_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCD1508786.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        11685 a         6065 c         7689 g        10335 t
ORIGIN      
        1 ttcatataat gaaatagcgg aagaattttt tgagcataat attcgccaaa aatttatcaa
       61 gtgataaaat tgttgtataa aatgattatt tcaggaggag gagttaaatg tttttagcat
      121 taaatgaaat tatgcattca aagttacgtt atgcgttagt tgcaggggtg atgtttttga
      181 tcgcctacct ggtatttttt ttgactgggt tagcttatgg cttagctcaa gataatcgaa
      241 cagcagtcga caagtgggag gcagattcga ttgtgctttc gaaagatgca aattcaaatc
      301 tagggatgtc catgattact aagaagatag cagaagaagt cgaaggtggg aaagtggcgt
      361 atcttgccca aacacctgga gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca
      421 atgttagctt cttcggtatc gataaaaatc aattcatcat gcccaatctt gttgaaggaa
      481 aagcttttga taacgatgat gaggcagtag gcgatattag tttgaaagaa gaatatggtc
      541 tagccatagg agatacagtt aagttatctg gtagtgacaa gacgtttaaa ctaactggct
      601 ttacggatca tgccaaattc aatgtctcac cagttctcta tacaaccatc aatgcttacc
      661 aagaaatccg cttcgaaaaa gaagatacaa gtgaaaatgc acggattaac gcaatagttg
      721 tcagaggtaa aatcagtaat ttacctgaag atttagaaca gatcaaaatt tctaaattca
      781 tcaatgaatt acctggatat aatgcgcaag tattgacttt tggatttatg atcggatttt
      841 tgatcgttat tgcggctatt gtgatcggga ttttcattta tgtactgacg atgcaaaaga
      901 ttaatatctt tggtgtaatg aaagcacaag ggatcacagg aggatttatt gcgcgttctg
      961 tagtggcaca aacattcatt ttatcatttg ttggtattct tcttggtcta ttaggaacag
     1021 tgggaacatc ccttgttcta cctgatgcgg ttccattcca aagcaattgg ctgttttttg
     1081 gcgtcattag cttgttgatg ttagtagtag ctgtgttagg cgcactattt tctgttcgga
     1141 caatcgtaaa aatcgatccg ttgaaggcaa ttggataaga aggaggaaga acatgaaagc
     1201 aatagaattt aaagaagtag aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt
     1261 gaaagaaaca accatgagta tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg
     1321 ttcaggtaaa agtacttttt tgactttggc aggcggattg cagacgccta gcagtggctc
     1381 tgtatttatc aatgatcaag aattcagtaa tcaaaaagaa agcgaacgcg ttaaactacg
     1441 ttttaaagaa attggcttta ttttgcaagc ttccaatctg gttccttttc tgacagtaaa
     1501 aaagcagtta ttattagtag ataagattaa acatgaacat cgtcaaaaag aagcaaacga
     1561 attatttgag cagcttggcg tggataagtt gatcaacaag tatccagaag aactatctgg
     1621 tggagaacgg caaagggtcg caatcgctcg tgcattgtac aatgatccgt ccattattct
     1681 agcagatgaa ccaactgcta gtttggattc agaaaaggca cgagaagtag tcgatatctt
     1741 ggcaaaagaa tcaaaagaca aaaataaagc aacgatcatg gttactcacg atacacggct
     1801 gattgacaaa tgcgatagaa tatttatgat ggaagatggt gtattgaaag aaaaagaata
     1861 accaaaaagc aggtggaaaa atggtccatc gatacaacaa agatacacag caaaaaattt
     1921 tgaaagaagc gactgacttg tttatgtcca agggatatct cggcacttca acaagagaaa
     1981 tcgcacaaaa agttgggatc acccagccca atctctatca ttatttcggt gataaagaaa
     2041 aactatatac ggcagtttta gaaaaccact taaaagatgt tggaaaagct ttgagagaaa
     2101 tcgttcaaac aagcgaaacg ggatttcaag taaccttgac aaaaatggcg cagtttttga
     2161 ttgagacaca tctggtcgat ttgtttctga tgcttcacga tcttgaaagc aatctttcaa
     2221 aggaaacaag agatcatctt ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag
     2281 aaatcttttc agcaaaccaa tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa
     2341 ggcatttttt cttagtgtta gcaccttaca tcactcggtc gaaaagacct gtggaagaac
     2401 cgctaaatgt ggcgcaattg atcgatttat atttgaatgg tgtattagga gaagcgtaaa
     2461 gctgttatat agggagaaaa tagttaggtg aagaagcaag atatgacgaa gatcgttata
     2521 tcttgcttct ttatataggt agtttttcat aaatacaaat ttgttattct ctcatgatta
     2581 tcagggtata tttttattta ttatgtcggt gttccaggaa gtgttttaat ttaataacga
     2641 agaaattaat tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa
     2701 tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt
     2761 tattttctac ggttatatct tttacagccc cttttttgct tacttaagct gattttaagt
     2821 tttggccctt agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat aattggagct
     2881 tttgtatttg caatcattat cattattatc tgttttcaaa tagatgtttt tggtcaaaaa
     2941 aagatagttg gacaaacggt cagcagtacg aacagtacgg ttacaaagac aaaggaatca
     3001 tttgatgata cagaacaaga gacattaatc aaaatcggaa aaaatgaccg tacactatat
     3061 acagatgact cgctaactac cccacttgca actataaacg gcggagagct ggcggagttt
     3121 ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg atggctatac tggatattta
     3181 gccctcgaag atggaaccaa agtcacaaag aatatacaaa caaaaccgga agagctatct
     3241 gatgcagtga ttgtattaga tcctgggcat ggcggaaatg atactggggc attaagcaat
     3301 gatgaacaaa cagaagaaaa agatataaca ttaagtacag ccataaaagt aaaaaaggcg
     3361 ctagaggatg ctggagcaac ggtttatttg acccatacta cagatgaatt agtacagctt
     3421 ggagatatat gcgactacag tgaagaaaaa aaggcagatg tctttatcag tttacacgca
     3481 gactctacgg aatatgcgaa tgaagcaact ggaatcacga catattatta ctatggacaa
     3541 gaggaaacat tagcacagac aattgctgac tccttcactg atctgcctct ggattcaaga
     3601 ggaatctcca ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc tattttaatc
     3661 gagatgggtt atatgaataa tgatagtgat ttaaaggaat tagtaacaga cagttatcag
     3721 cagcagattg ctgcaagttt gacacaagca ttgaccactt attttcagta ataaataagc
     3781 ccagccaaac aaaaatagct cctcatattt ttcgtttggc cgggcttatt atcgcagatg
     3841 caagctttta aacaccgttt aaggaagagg tcgtgagcct gccgttcaac tctgagtcac
     3901 aaggagcaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact tgtgaccgag
     3961 gagttggcat gagaacaccg tttaagaaaa aggttgtgaa atcaacgttt tgttctgagt
     4021 attagagaaa caaacggaaa aatagttcct catattttgc cgtttgttga gctaataccg
     4081 aggatcagat gattgaacac cgtttattcg gaacaaggga ctgtgacatg acttttgtca
     4141 cagttccatt atttttttag tagcttcaaa gctttttctg tattggcaaa atgaagtttg
     4201 gcatattttt caagcatagg catgccgcct ttctccaata gttttgccgc tacttggtca
     4261 gatttcgttc cagctcctga cggaagggtg attccagctt cagctgatgc tttgtctaac
     4321 tcttccaaaa agctggcacg actgatgata gaagcagcag caacagctaa atgatattgt
     4381 tctcctttag taacgaagta cagtttttct tgtaattgat ttttttctaa acgcgcatat
     4441 tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc
     4501 tcgatttttt ttagcagcaa atggatcgct tgattgtgca aagcgacttt catccgaacg
     4561 gcattgtatt ttggttggat ctcattgtat ttttctggag ataagacaag cagtttatag
     4621 gggatggttt tttttaagat gtcagcaagc tggatgattt ggctatcttt taactctttt
     4681 gaatctctaa caccaagttt acgtaattct gcaagctgat cacgtttcac ataagcagca
     4741 catactgtta gcggaccgaa atagctgcca tttccgacct catcactacc taaaacggat
     4801 aattgactga tattttttgg caaagaagag gctgtttttg ctggcttggc ttgtttatcc
     4861 tgtgcctccc cccatttatc agcttctatt gctgcattcg taccttgaaa catcactttt
     4921 ccggatgtat aagcggtaat ggtcgtcccg tctttttttg cagcaaagct agtataagga
     4981 atctgtttat ttaacaaata agagcgatag tatgctttca tttgactaag ttggtcgttt
     5041 gttgctttga taatgacgtt gctcataaaa ttctcctcgt ttttatacat aaaactatac
     5101 cgcaactcac gacttttcgt tttctaagag attgcgagac atagcatttt tttaaagttt
     5161 atcatataat agttaagtat gtgaaaaata tttgtggaga acggtgaaag aaccaacttc
     5221 atggtacact aaataaaact ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa
     5281 aaacacgcta caaagcggtg attgccaacc aaacatatac tattatcgga cgggaaacca
     5341 agcatcatat ggatatcgta acgaagctga tcaatgaaca gttagcagaa ttgaaacaat
     5401 tatcaccaca aatggataat gaacaagcag caatcttgat ggctgtcaat gcgctttcgg
     5461 atcaattgaa gaaacaagaa agaatattgg agctggaaga agaaacagca gaattaaaga
     5521 aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga
     5581 acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg
     5641 tggaagcaca acagatttta aacgagaaac gaaaaggaca aattaaacaa aaatcttcta
     5701 attaagatcc ttttatcaaa ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta
     5761 gcatgctgag tttgctgatt ttatttttat tgctaattgc gtttttttct ggagcacgtc
     5821 gcgggtttgc tttgcaaatc atattcgcga ttggatatgt attgtcattt attgctgccc
     5881 agcattttta caaaccatta gcaagtcatt tggaattata cattccatac ccagctgtca
     5941 ccccaacatc aaagttagca ttttttgatc aggtatttgc ctttcaccta gatgaagctt
     6001 tttatgcagg aactgctttt ctgattattt tgctaattgg ctggctgctt acccgatttg
     6061 tgggggtttt tgtccatgga ttgacgtatg tgccgatctt gagacaggta gactggattg
     6121 caggaggcat cctgtcacta atcatggctt acgttactat atttttgatt ttgcagttac
     6181 ttgcctttgt tccattagat gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt
     6241 ttatcgttga gagaacgcca tttcttacca acaagatgta tgagctttgg gtaacgaata
     6301 ttttgaatta aggaaaatag tgctggaaaa tatttcgttt tttgtacaga gtagtttatt
     6361 taagaaggct gcgtagcgta tgtccggcac agctcttttt tcatagtgaa agataaggtg
     6421 ataaaatgaa taaccgaatc cttgaaacat tggaatttga aaaagtcaaa cagatggtca
     6481 gacaatttgt agtgactgca caaggaaaag aagaactggc agaattggta ccagtgagtg
     6541 agaaacaaac gatcacaaac tggcttcaag aaactgagga tggattgaaa gtgcaacgtc
     6601 tgcgaggcgg gatccctata cctaaaattg aaaatatccg tccccatatg aaacggattg
     6661 agatcggagc agacttgaat ggagtggagc ttgcacaagt ttccagagta ttgtctacga
     6721 caagcgaatt gaatcgtttc attgatgatc tttctgacag tgaaatcgaa tttgcacgtt
     6781 tgtatatgtg ggcagatcag ttagtcacga tccctgtttt gagccgccgt ttaaaggaag
     6841 caatcgatga agacggacgc gtgactgatg acgcttctcc tgagttaaag agcataagac
     6901 agaacattcg gcgaagtgaa caagctgttc gtgaacagtt agatgggatc gtccgtggga
     6961 aaaatgcaaa gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc
     7021 cagtcaaaca agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag
     7081 gtcagacttt atttattgaa ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat
     7141 accaaatcgc tgaacgaaat gagattcaac gtattttaag tgaactttct gctgaacttg
     7201 ttccccatcg tcaagaaatc atccacaata cttatgtgat tggtaaaatg gatctgatga
     7261 atgcaaaagc acgatttggt aaagaagtaa aagccatcgt gccgggaatc agtgaagaca
     7321 atcatgtcgt cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa gtagtttcta
     7381 atgacatcac aatcggaaaa gactatcaag ccatcgtcat tacaggacct aacacaggtg
     7441 ggaaaacgat cacgttgaag actttaggtc tgttacaact gatggggcaa gcaggattac
     7501 cgattcctgc aggtgaagaa agtcaaatcg gtatttttga ggaagtattt gcggatatag
     7561 gagacgaaca gtcgattgag caaagtctat cgaccttttc ttctcatatg acgaacactg
     7621 tagatatttt atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag
     7681 gaacagatcc gcaagaaggt gctgctctag caatcgcaat cttagatgat cttggtaaaa
     7741 agtctgctta tgtgatggca acaacgcatt atcctgaatt gaaagtttat ggatataatc
     7801 gggcaaatac gattaatgcc agcatggagt ttgatgtgga tacattaagt ccaacgtatc
     7861 gtttgctgat tggcgttcct ggacgaagca atgcgtttga aatctctagc cgattaggtc
     7921 ttgatacaga agttattgat gaagcgaagc agttgatgaa tgatgagagc caagatttaa
     7981 acgaaatgat cacagattta gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa
     8041 tgcgtcattt tgtttctgag gcacaagaac ttcacgatga tttgaaagaa gcttacagct
     8101 atttctttga agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca aatgaagtcg
     8161 tgtcagaggc ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc
     8221 agattgggca aggaaatgtg aaagagcatc agttgatcga tgcaaagacc caattagcaa
     8281 atctacatca agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa
     8341 aaacgttgaa accgggagac gaagtgttgg ttacgactta cggccaaaga gggacgcttt
     8401 tacgtaaaaa cgggaatcag tggcaagtag aaatcggtat attaaaaatg aatgtgtccg
     8461 aagacgagct gacgcctgtt gctcctcaaa aagagccgac acaacgagtt atccatgctg
     8521 tacgatctga atctagtagt cacgtgccga atcaattgga ccttcgagga aaacgatacg
     8581 aagaagcgct aagtgaggta gatcaatatt tggattctgc cattctagct ggttatcctc
     8641 aagtgactat tgtccacgga aaaggaacag gcgcacttcg caagggaatt actgattatc
     8701 ttaaaaacca tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg
     8761 gtgcgacaat tgttaaattt aagtaaagct tactaaaggt aagcagatgg ttcgctatcc
     8821 aagtatagaa atgctatact agtcatagat tttaaaaatg gaggtctttg atatgtcaca
     8881 agtaattaca gataagacat ttaatgaaga aacagataaa ggacttgtat tgattgattt
     8941 ttgggcaact tggtgtggtc catgtcgtat gcaagcaccg attttggatc agttggaaca
     9001 agaatatgat gaagaagaat tcagaattgc taaaatggat gtggatgaaa atccagaaac
     9061 tcctcaacaa ttcggtatca tgagcattcc aacattaatg ttgaaaaaag atggtcaagt
     9121 agtagaaaaa gctgttggtg ttcattcaaa agaacaacta cgtcaaatga tcgatcaata
     9181 tttataaaaa cttacagcaa aaataagctg agggtgggac tgaatccagt tccgtcctcg
     9241 gtttttttgc ggttgagtta aggttattca aattaatatt ttttgagtat aattacgttg
     9301 tcaggctgga ttgttttttt gacagacatg ctataataac tttaattgaa tatgaagggg
     9361 agtaccagat gaacgaacga atcaaaaaca aattagcttt acttcctgat cagcccggct
     9421 gttacttgat gaaagataaa aatggaacga tcatttatgt cggcaaagcc aaaatattaa
     9481 aaaatcgtgt ccgctcatat tttactggta gccataatac gaaaacggaa cggcttgtta
     9541 gtgaaatcga agactttgaa tacatcgtta cagaatcgaa tatcgaagca ttgctcttag
     9601 agatcaatct gataaagaaa aatgatccta aatacaacat tatgttaaag gatgataaaa
     9661 cctatccttt ccttaagata acgaatgaaa aatatccccg tttggtgatt actcgtaagg
     9721 tactaaaaga caaagcactg tattttggac catatcccga tgtcggtgcg gcgaatgaga
     9781 caaagaagat cctcgatcga ttatttcctc tgagaaaatg caaacctagt cagacgaagg
     9841 agccttgtct ttactatcat ttgggacaat gcctgtgtcc ttattatttc gacgtagatc
     9901 cgaaagtcta tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga ggacacgaaa
     9961 aaatcgagtc agagatccat cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa
    10021 aagctgcaga ataccgagac cagatccgtg ccattgagac aatcatgact cgccaaaaaa
    10081 tgacaaatac tgatttactg gatcgagatg tatttggtta tgcgattgat aaaggatgga
    10141 tgtgtgtcca agtgtttttc gtaagacaag gaaagctaat tgaacgagat gtttcaatgt
    10201 ttcctttcta taacgaagca gaagaagatt tcttgactta cctcggtcag ttttatcagg
    10261 aaaatgagca tttcattcct aaagaagtct tgattcctga tacgatcgac aagcccagtg
    10321 tagaagcttt gttagctacg aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag
    10381 ttgaattggc gagtaagaat gcctcagttg ctttgaatga acggtttgat ctgattgcca
    10441 gaaaacagga acggacgatc ggagcagttg aacgtttagg aaacgcgatg aatatcccga
    10501 ccccaatacg tatcgagtct tttgataact ctaatatcat ggggacgaat cctgtggctg
    10561 cgatggtcgt attcattgat ggaaaaccgg caagaaaaga atatcggaaa tataaaataa
    10621 aaacagtcaa cggaccggat gattatgcat caatgagaga agtcatctat cgcagatatt
    10681 cgcgagtgat tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat ggaggaaaag
    10741 gacaagtaga tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat attcctattg
    10801 ctggcatggt aaaaaacgat aaacataaaa caaatgagtt gctattcggt acagatttgg
    10861 ctgtagtacc gcttgagcga aattcaccag agtttttctt actccagcga atccaagatg
    10921 aagtccatcg attcgcaatc actttccatc ggcagctacg cagtaaaaac agttttgctt
    10981 cccgattaga tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg cttaaagagt
    11041 tcaaatcgtt gaaaaatatc acagcagcca cgacagaaga attacaagaa atcggactgc
    11101 cgaaaaatgt cgcacaaaac gtatttgata aattacatca ggaatgagga aagggcactt
    11161 tacaaagaaa gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt ttacgttttg
    11221 ttggtaaata ataaatcgct tacgtctggt gtctaaggat atatgtgatt tcttgaagaa
    11281 ggagtgaaga aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac tacacagctg
    11341 tcgatcatgt ctctttccaa atccccgatg gaaaaatcat ggggctaatt ggccaaaacg
    11401 gggctgggaa gacaacgact tttcgtttga tcttggattt tttgacacaa gaccaaggag
    11461 aagtgttatg gaatggccat catcttgggg agaaagatta tgatgtcatc ggttatcttc
    11521 cggaggaacg gggactttat ccgaaagtaa cgattcaaga ccaactgctt tattttgcag
    11581 aacttagagg gaaatcaaga aaagagattg aaccaaaaat cgacttttgg atggaaaaat
    11641 ttcaggtaaa agggaaaaag acggataaag taaaatcttt gtctaaagga aatcagcaaa
    11701 aagtccagct gatcgccacg ttaatccatg aacctaaatt gatcattttg gatgagccat
    11761 tcagcgggtt agatccggta aatgctgaat tgctaaaaga tggaatattg gaattgaaaa
    11821 aacatggttc atgtgtcatt ttttctagcc acaatatgga taatgtagaa aaaatctgcg
    11881 atcatttgat catgttgcga aacggaaaaa tggttttgaa tggaaaagta catgagattc
    11941 gtgaatcatt tggccgtacg aagttattct tggaatctgg tttaagccaa caggaagtag
    12001 aagaaatcga aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta gagattacct
    12061 tagaagatcc tgaagctggg aaagaaatct ttactcgtgc gactcagttc ggctacattc
    12121 ctatgtttaa ccagcaaccg ccaactttgg aagagatttt caaattgaaa gcaggtgaaa
    12181 tcgatgcgta agttttggat tattaccaaa gatgtctatt taaaaaatgt taagtcgatt
    12241 tctttcttga tcatgatatt agtccctttt atcatgctgg gtatcattta tgtagcagga
    12301 aactttgctc aacaaaatag tgatgtcaat aaaatcggcg tattggctga taatgagcaa
    12361 atcagtcagc aaatgagcca agtcaaaaca gatgattttg agttcaaagc agttgattct
    12421 gaagaaaaag cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt agtagtctca
    12481 actgagaatg ataagataaa tgggatgctt tatagcgaaa attcattagg acaaagtaca
    12541 caactactga tacaacaaca actgactggc ttgcaatcga tgcttcgagc gagtcaatta
    12601 ggcatttcac cagaagaagt agctagcttg agccaacaag caagtttgac tcgacaaaaa
    12661 gtcaattttg attcaaaagg aaaaatgaca gtcggagaag ataactcgac agtccaatac
    12721 gtcgtaagtt atgtagcaac gattgtcttg ttcattatta ttttgacgta tgcgcagatc
    12781 attgcacaag agatcgcttc tgaaaaaggc acgcggatca tggaagtgat cctgtccagt
    12841 acgactgcac aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt tgcattaacg
    12901 caaatggcat tatacggtat tatttttggt ataggataca gtcaattcaa agatatggaa
    12961 atggtcaaaa atatattaga cggtatctca ttggataact tgattggacc attcttgtgg
    13021 tattctcttc tattcatgat ctttggcatt ttgatttttt ctgttttagc agctttatgt
    13081 ggctctttgg tcaacaaagc agaagataca gcaaaagcaa ttttgccagt gacttatcta
    13141 tctctaggcg gatacatgct tggattgata ctaggagctt cagatccaaa caatatcgtt
    13201 attcggatca cttcgtatat tccattctta tcttcatata ttatgccgat tcgcttagca
    13261 aatgagacag tcggtatggg tggagcattg atttccttgt tggtacttgt agccgtaaca
    13321 ttcttattga tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt ttatagcgaa
    13381 ggcggattat ggagttcatt gaaacaatcg atttcaatta tgaggaatga acgaaaaaaa
    13441 gcataaaaca atcctaaaat acagaaggaa gctggacatc tattgttccc agcttctttt
    13501 tgtatagacc tattttattt attttactat attggatata tattcattga aaatagtgta
    13561 taacaacttt ataatattga gtataccaat ttttggaggc gttttaatgg accgttattt
    13621 agatttactt tcagaaaaat tcccctcaac ggaggatgtc atcacggaaa tcatcaatct
    13681 cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagtgata tacatggcga
    13741 atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa
    13801 agaccttttt tctgaacaaa tgacggaaac ggatatcagc caattcacta tttttacggc
    13861 ttatccagaa tatgcattaa cgactgattg gtacaaagaa caagataaat cgcagctgat
    13921 ccatcatttg atcgatcttt tacggtttac gacagtcaaa tatacccggt caaaagttcg
    13981 aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgaccg
    14041 tatcaatgga aaaaaagact atgtaaaaaa aatcattgaa caacttgttt tgatgaagga
    14101 agaagaaaaa tttttaacga aacttgcaca gacgatccaa aaatctgtca tcgatcattt
    14161 gcatatcgtt ggagatattt ttgaccgagg aactcaagca gccaaagtga tggatcgaat
    14221 catggatttt tcttctgtag atatccaatg gggaaatcat gatattttgt ggattggtgc
    14281 gtattgcggc tctgaggcat gtttactgac acttttacgg attgcagcac gctataatta
    14341 tttatttgaa ttagagaaag aatatgggtt gaatttacgt ccattgtttt cttttgctca
    14401 tcaaacttat cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc tatccgaaag
    14461 agagcaagaa gaattagaaa aaatccatca ggcactttct atccttcaat tcaaatcaga
    14521 acatcagtta ttggatcgtc gacctgaatt taaaatggac gatcgtaaat tgctggaaaa
    14581 aatcgactat gaagcatcaa cgattgattt agaaggtcag ctctatgagt tgaaaggaac
    14641 ttgttttcaa acgatccagc cagatgatcc aaaaaaattc ttggaagagg aacaaaaagt
    14701 gattgatagt ttgatgtatt cattccagca ctctttgaga atgcaaaaac atatgacgtt
    14761 tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtgctgt ttcatggctg
    14821 tatacctgta gacgagaagg gacagctctt agcatttgaa ttagaccaat cttatcgcgg
    14881 aaaagaactg ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag atctgactgc
    14941 aaaagaagat ttagcaaccg atttgatttg gtatgcgtgg agtgggccat tttcgccttt
    15001 attcggcaag agtaaaatgg cgacttttga acgttatttt ctcacagagc ctcatacgca
    15061 cgtcgagaaa agcaatcctt attatcactt gcgagatgaa gagtggttca gtgaaaaaat
    15121 tttagcagaa ttcgacacaa aagaagaagg gtcagcaatc attaatgggc acacgccagt
    15181 aaaagtaaaa aaaggggagt caccgatcaa agcaaatgga aaagtatttg tcattgacgg
    15241 aggtttatcc aaagcttatc aaaaaacgac aggaattgcc ggttattcac tattatgcaa
    15301 ttcttatggt ttccaaatcg tgacacatta tccgtttttg cctattgaac aacaatttgc
    15361 aaaaaaatcc gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac cacgcaaact
    15421 caatcgtgat acaacaaaag gtatggaact gcagcagcag attacccagc tcaaaaggct
    15481 gttggactat cgaaaggacg attgagaaga attccagtga acattgttcc atagactgga
    15541 gactgagaca aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt cttctgccaa
    15601 ctcctcggtc acaagtcaca ctattcaaca aacatgaaaa gctgtttttt gaaattgctc
    15661 cttgtgactc agagttgaac ggcaggctca cgacctcttg gtataaacgg tgttcaaaag
    15721 ccagtttctc ggtattaagc caaatttatg caaaaatgtg aaaagccatt ttcacaaaat
    15781 ttttcttaat actcgaaact aaacagcttt ttcacaaccc cctcagaaag tctattaaaa
    15841 ttcattcaca aagaaatcga ataagcgctg ttcagatgga tgtacacggt gggtaagttc
    15901 cggatgccat tgaacaccga ggattttatg catatcatct ttgctttgaa ccgcttcaac
    15961 gagtccatcg ttagaaaaag cgatggcttt taataaaggt gaaagatctt tgactgcctg
    16021 atgatggtaa gaattcacaa aatatggatc ggggagaagg ttgcttaaca aactgtctga
    16081 aacaatcttg acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt catgttttac
    16141 tgaccacttt ggatataatg aaagatcttg ataaagtgta ccttccaaaa cgacattcaa
    16201 tagttggagt ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg cttccaagat
    16261 cagtgcctgt tcaaattggt ctcgttgaat atttgtttcc gttagctttg gatgaggatc
    16321 ttccatgtaa agttgtggac tgacatcttg accacctgca agaataagtt tgtcgatttg
    16381 tccaatcagt tgaggagctg aatctggaga catgactggc agaataagag gcaatccttg
    16441 agcatctaaa actgcatcca cgaatccttg cggtgtatag ctcacttgat ttccttggaa
    16501 agtatctgtt gctcgaatca gttgatttcc ggcaatgccg atgattggtt tcatttgtat
    16561 tccccctaaa gtagacttat tgtaacaagg atttaaaaaa aattaaacta tgtaagactt
    16621 gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaaatt tcgtgtgaaa
    16681 ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa
    16741 aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt
    16801 gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa
    16861 gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa
    16921 atccgaaaag cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca gcagctgaag
    16981 atctatgcaa cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta tcgacaacaa
    17041 ctaaaacagt tggtggaaga aaaagaacaa tttgaaatac aagaattaga aaaagaaaaa
    17101 atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagatca atatcaaaag
    17161 catcagagtc aagcgatcgg tgcgatcatt gaaagggtga aagaaactta tggccgtcac
    17221 taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc
    17281 agtacaagga atccaaaacg ttgaaatccg ggatctgtat caaaatagaa caaacaacca
    17341 gtgggtggag aagtattttc cagataccac gattattgat aaagaagcaa aattaagcgc
    17401 tttaggtaat cggttgaaag acatccaaga agctatccaa ttcatacaac accacggcga
    17461 tagccgtcaa aagaaactcc atttgaaacg tcaagaactt tccttacaag atttggaagc
    17521 caattattcc gaagaaaatt tcataaataa attgaaagaa gtccttgatt taaaagaaaa
    17581 gtgggaacaa ctaactgaag aacgtgatcg tttagaagaa gaggaagaat ggcttcttca
    17641 ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat
    17701 ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactgagta agcttgatga
    17761 cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt
    17821 aaaaaaatca gttgctgtta tagaagaaat tgctgggaaa tacgggttca ataaagaaac
    17881 gtatccttat gaacaaaatc ctaaagaaaa actgcaagag accaagcagc aattatccat
    17941 tgttcacgag cagcaaaaga agataacgac agcgttaggt cagtgcagtg gctatatcca
    18001 agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgagaaa aaataaaaga
    18061 acggtttatc catgcgactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa
    18121 acagaatttg ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat acgtcagttt
    18181 cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc
    18241 gcttgtcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt
    18301 agatccaaca ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga
    18361 tgttggttat ggtttgctga tgctcattgg gacaatacta gctcaaaaac tacttgttct
    18421 accacgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac catcgatcat
    18481 ctggggattg atctacagtt catttttcgg acagtcatta ccaaaagaac tgtttggcat
    18541 ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc
    18601 cgtgattttc ggtctgatac agattttggt tggattattt gtggcagcaa aggaaaatat
    18661 caggagaaaa gattacttga gtgctgtcag tgatggattt gcctggcaag ggatcctaat
    18721 tgggatcgtt attgcgttgg ttggtgccat gttgataaaa aatacagcat ttgtttacct
    18781 tggcgctggg ctatcgatca ttgctgcctt atgtatcttg atcgttccga ttatccaaac
    18841 gccgtctaaa gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac tgaccggcta
    18901 tatcggtgat ttagtcagtt atacacgttt gatggcattg ggtatttcag gcggaagtat
    18961 tggagcggca ttcaatatgc tagttgcttt catgccgcca gctgctcgat tcagtgtagg
    19021 gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgc taagtgccta
    19081 tgtccatggc gcacgattgc agtatgtcga attttttggg aagttttata caggaggcgg
    19141 acgtgccttt gatccgctga aaacggcgga aaaatacgtc aacatcaatc acaagaaaaa
    19201 aaacaataaa taattggagg atttttatat aatgatggat tacttaatta gtcaaaatgg
    19261 tggaatggta tttgcggtat tagcgatggc aacagcgacg attttttcag ggatcggttc
    19321 agctaaaggt gttggaatga caggtgaggc agcagcagca ttgacaacaa gtcaacccga
    19381 aaaattcggg caggctctga ttctgcaatt gcttccaggc acgcaaggtt tgtatggatt
    19441 cgttattgcc tttttgattt ttatcaactt aggaaatgac atgtcagttg tacaaggact
    19501 gaactttcta ggtgcttctc tgccgattgc ttttacaggc ttgttttcag gtattgccca
    19561 aggaaaagtc gcagcggcag gtattcaaat tttagcgaaa aaacctgaac atgctacaaa
    19621 aggaatcatt tttgctgcga tggttgaaac atacgcgatt ttaggtttcg ttatttcctt
    19681 cctattagtg ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa
    19741 atcatctctc agatgaacga agcagcagag caggaaagag cggcgttaga acaagaagag
    19801 cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac agaacatcaa
    19861 aaacaaaaag aaaaacaaat cgaattactc gaaaaaaaat accgtcagct aaggaatcgc
    19921 cagcaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt aagacgattg
    19981 tttgcagatg cggtaactga aatggaaaat tgggatgaat ctgaacaaat ccagtttatc
    20041 aaaaatgcat tgtattcatt acctttgaca ggaaaagtag cattcattgc tggagaaaaa
    20101 tcagcagcct atctttctca gacgttgttg gatgaatgga acaatgaact tccctttatg
    20161 atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga
    20221 gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt
    20281 gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa
    20341 atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga
    20401 ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat
    20461 atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc
    20521 tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc
    20581 gttatacttt ccataatctg aaggttttga caaaagctga gatgacaaat caaaatctgg
    20641 accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata
    20701 cgcaagtttc aaatgaactt cctgaaagca ttatggacag tatacgagaa gtacgtgatt
    20761 attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga
    20821 cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg
    20881 cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat
    20941 atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga
    21001 actttgtaga taaagatatg gaagattaca cgaattttct attagagaca gattatgctg
    21061 attttctgcg ccctgtcatc cgtgagggaa atattgatct cgtaaagctt gaacagttga
    21121 aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc
    21181 cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta
    21241 tcggcaaaag aagccatttc gaatcagaaa caatcagaaa gcgggtgaga caagtctatg
    21301 acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc
    21361 tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca
    21421 aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg
    21481 atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga
    21541 tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat
    21601 atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc
    21661 ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg
    21721 ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa
    21781 gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct
    21841 ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca
    21901 ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc
    21961 gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg
    22021 agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg
    22081 gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat
    22141 gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa
    22201 tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc
    22261 actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta
    22321 cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat
    22381 gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta
    22441 aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta
    22501 ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga
    22561 atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc
    22621 acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt
    22681 gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga
    22741 agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta
    22801 tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa
    22861 gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg
    22921 atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa
    22981 gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg
    23041 aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta
    23101 gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat
    23161 acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa
    23221 gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta
    23281 tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta
    23341 gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg
    23401 agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga
    23461 aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat
    23521 tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg
    23581 aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact
    23641 aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa
    23701 cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt
    23761 agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa
    23821 tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga
    23881 attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt
    23941 agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg
    24001 tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat
    24061 cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa
    24121 gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga
    24181 gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac
    24241 gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac
    24301 tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac
    24361 tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca
    24421 gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg
    24481 aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg
    24541 aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa
    24601 gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac
    24661 gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag
    24721 aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg
    24781 agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa
    24841 acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat
    24901 gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc
    24961 tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat
    25021 cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat
    25081 catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc
    25141 tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac
    25201 taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc
    25261 gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca
    25321 attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt
    25381 aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa
    25441 aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag
    25501 cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca
    25561 ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct
    25621 gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt
    25681 caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt
    25741 ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa
    25801 gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg
    25861 gcctttgtga tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt
    25921 ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca
    25981 gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg
    26041 gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg
    26101 gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt
    26161 acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta
    26221 gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga
    26281 acggctggat attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg
    26341 atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact
    26401 acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa
    26461 gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt
    26521 acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa
    26581 acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt
    26641 acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg
    26701 tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga
    26761 acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg
    26821 acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat
    26881 actttaagtg caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt
    26941 agtgcctctt ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt
    27001 tataattcat atttttttat tttttctttt acgatactta gtccagaagc taaaatttca
    27061 aggttattag aggataggga aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag
    27121 cgtctggaat gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg
    27181 tcaatctcag gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct
    27241 actttgaaaa gagaatcaaa aagctgattg gatctttttg taagacttaa tttagtttct
    27301 aagatcctat ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca
    27361 gaagttttca catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga
    27421 actacaagat aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag
    27481 attgtttggt taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt
    27541 gttaaaaatg aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg
    27601 gctaattgct ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc
    27661 ataagaaaga caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc
    27721 attccttgct cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca
    27781 atgaaattag cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca
    27841 gaaagtgtga tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac
    27901 ggaatgactc cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct
    27961 ttgggatctt ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata
    28021 aacgggacaa cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata
    28081 tgttcggtta cttcattggt agagatggtg atggactcaa agacattggg agaaataaat
    28141 gtgccacttc caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg
    28201 ccaacggtag taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga
    28261 gtgtttttct gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga
    28321 taataaggac gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa
    28381 gaattaatag gcattttctc cactccttaa aattgtatac catacaattt taccattgat
    28441 tgtattgatt ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat
    28501 tttgaggaag tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt
    28561 ttttttgatt atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct
    28621 gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat
    28681 tttggcacaa aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat
    28741 ttaggagaga ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca
    28801 aatgcagtca cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca
    28861 aatccgcatg cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg
    28921 actgcgattt ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa
    28981 agacgaaata cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat
    29041 gcaatatcag tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt
    29101 aaagaggcat ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc
    29161 acatttactc ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat
    29221 gtagatgatt actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa
    29281 ggagatttag aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa
    29341 acattttata aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa
    29401 gatctttgtt gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact
    29461 tagattatta cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg
    29521 tttatgtgct agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca
    29581 atttatctga aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta
    29641 cgccagatcg agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat
    29701 ttaactatta caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat
    29761 ttgtgacaag aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag
    29821 tgccgatatt gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag
    29881 cacgtgattt atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt
    29941 ttacagctat caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc
    30001 aaaaagactt tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat
    30061 gtggatcagc acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat
    30121 tgtcagctta tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc
    30181 gtgtgataat aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc
    30241 tttcaaatga attaaaaata aattggtgga gagtggcaac cttatagcta caatggttgt
    30301 taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg
    30361 gtgtttgttt tttattcaat caagagtaaa atgccccata ctaactatcc tcggatgact
    30421 tcgattttat aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat
    30481 ccttttaagt cagtaatctc aagaccagca gctttatgtt gctcgtgtaa tttttctaga
    30541 tcatcagctg ctatagcgat atgaccatag ccgtctccta gatcatacgc accatgatca
    30601 tagttgtaag tcaactctaa ttcatagtca tccccaggaa gtgtcaaata aacaagtgta
    30661 aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc cttttgataa
    30721 aattcgattg atgcttcaag atctttcact cgaacacatg tgtgagccat tttcatagat
    30781 tcatctctcc ttttcctttt actcaatcat aacaaaaaga agtaaggaaa tagttgattt
    30841 gtgcttgttc atgagaaatt tctcatgtaa aatggataga aaataaaact gttaaggtgg
    30901 aaactattca tggataaacc aatttttggt gaaaaaaagc aatttgctga ttatcaaacc
    30961 cgttatgcag catatattgt catagaaaga gatcagcaga ttgcagtaat cgaggctcca
    31021 aatggtgctt tttttcttcc tggcggggaa attgaaggag aagagacgaa agaagaagcg
    31081 attgtccgtg agctgataga agaaatggga attgtggcag aaatttcttc ttatctggga
    31141 gaagctgatg agtattttta ctcgaattat cgtgcaactt attattataa tccaggatat
    31201 ttctttgttg ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac caataaaatt
    31261 tggtgggtga gccctcaaga agcgcttgtg aaattgaaac gtggtagcca ccgatgggca
    31321 gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc agctttctct
    31381 cagttttttt atttattaca gtacatattt tttgattgct tcagctacac cgtgctcgtc
    31441 gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga gaaacggcat ttcccattgc
    31501 tactcctaga ccagcatatt caatcatgga taagtcgttc tcgttatctc cgatagccat
    31561 gatttcatct tgatcaatac caaggtgctg tgcgagattc gcaactgccg cacctttact
    31621 tgcttcttta tttagtactt cgaagtaata gggcgcactt ttgaccgttg tgtatttatc
    31681 acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat
    31741 catcattttg acgatgttca tgtctggtgt catttcttca ggtgttcgat attttagcgg
    31801 catcttgact aaacttgctt cgtggatcgt ataagcgctg atatctcgat taggcgtgta
    31861 gatcgctttg tcatcgatcg aatggagatg actgccgaca cggcgtgcca ttacttcgat
    31921 ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg attttacctg atttcgtttg
    31981 ttgtacgagt gccccgttat aagtaataac gtaatcatta tcttgaaaaa ggttcaactc
    32041 gtttaactgt tcctggacac ctggtaaggg acggccagtg cataaaacga tcgaaacacc
    32101 ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt gttacttctc ttttctcatt
    32161 taataaagta ccatcgatgt cgatagcgac tagtttgata gacataattt cctccaatat
    32221 attttaattt atttcaatca atgcgccatt acggatatgg ctggcaaatt cttgataagt
    32281 ttcgtcaaat aaatcataat gatcacgcaa ggacggatca accatttctt ttggaaagaa
    32341 aaatcgctca tctccttgtt cttgtcccgc taatgcagca acaagctgac taacctgtga
    32401 taattctatc aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg
    32461 ttctgggcga taaaaatcat aaggtagatc gtagctagaa ttcacagcag tgtaataaac
    32521 aggattatat ccgacctttt ctactaacaa agtgagttct tggactaatt ttgaatcccg
    32581 gttgccagaa aaagtagcag atttcagtgg tttacgattc aaaaaacgtt tagctaaatc
    32641 atttaaaata ggatcagcag attcagacca ttgagtaaag taagtgccca agaccccatc
    32701 atcaagtttg aggtattctt ctaaagtaaa gcttccgtca agaaatggca gaagtaattg
    32761 agaatggagt tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag
    32821 atgctccaaa attacttcca taccacggga aacaggatgg aagtacactt gcacatacat
    32881 ttgatatcgg ctaacgatat aatcttccac tgcatgcatg ccactcatgg aaaaagcgat
    32941 cccgccttca taaggacgaa tcacacgtaa tattcgggta agatcaaatg ttccatattc
    33001 tgttccagtg aaataagcat cccgaagcaa atagtccatt cgatctgcat cgatctgact
    33061 agaaatcatt tggacaactt gtgggttggg gtaagttttt tggatcacac tagctacctt
    33121 ttcaggaaaa ccttcttcta ctcggttaag aatctgatac acttctgttt ctggagaagt
    33181 gatgatctct acagtgatgg cttcatgatt cgtatggaag atatgttcaa atgtatgaga
    33241 gtaaggacca tgaccgacat catgcaacag tgccgcacat aaagtcacca gccgttcctg
    33301 atcatcccag cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa agatatcgca
    33361 gattcgtctt gaaatctcat aaacgcctaa tgaatgagta aaacggctat gttctgctcc
    33421 gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt
    33481 cgaattgatc aaatctaaga ttacttgatg ctggacatgg atatagttat ggactggatc
    33541 acgaaaaact ttttccatag gcagcatttg atatttataa ggggttgtca taatgtagct
    33601 cctttatgtt attacgttgt tccattctct ggatttggtt gttccattgc tccagctgga
    33661 tttctttttt tagccagtcg gtagaatcct gtaaatcact tgcagaatac agcttttcaa
    33721 aagcttttgc aaatgcttct ttgacttctt ttacaaaaag gggttggtca agcagttctt
    33781 caagtgttgc cattgtagca ggatccactg caggatagcc atttgtacca aagtcttctt
    33841 ttaaagcaga ttgataaaat cgcttcatga cttgcccacg tttttcctga ttaccgttga
    33901 cacttaagta catcataaca gaaatcccat ttttgatccg acgctgagcg ataccggcaa
    33961 attttttgcc atggatgctt aaatcataag ttccaggaca ataagaatgg gtgatttcga
    34021 atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag
    34081 cgtcatctat ggttattttt tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa
    34141 ttccttcatc tgatacgatc cctaatccac ctgaatttct gatcactgga gcgtaacctg
    34201 catcttccag tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc ttcataccta
    34261 agatgactgt gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt tctttagaat
    34321 attccgttaa tatatctgtt aatgcaaaag gggaaaagtc gtttttttga taaatgtgct
    34381 gatctagtcg aatcgcaggg ttcattcaac cggctccttt gtataaattc atattttcta
    34441 caatattata ctctaaaatc aaagagttgt tttggaaaaa aggttttctg taaaaatgga
    34501 caagttggtt gacaaaacaa aagaaactag gaacaatgta gctatgagaa gtaaaggaga
    34561 atgaataatg gaattatcac aaggaacacc gatttcaatc aaattgcgta caaaagtaaa
    34621 acagcaaggg gaagtgcaag atttttattt tgatttgaaa gggcaaatgg tgacaattgg
    34681 cgatacgctg tacatacgtt ataaagagat tcaggaggag acgggcgatg agattcctgt
    34741 aacgatcaaa ttagttccag atggtcatgt acagctgatc cgtgcaggag aaatgcggat
    34801 gcgtttgaaa tttggctata aagaacggtt agaaacatcc tatcgaacac catacggtat
    34861 gatccagatt gctactttta cgaaagaact gcatgttagt ttgaaagacc gtcccacagc
    34921 agggaaggtt cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg gcgaatatta
    34981 tttgaccttg gattttacag cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt
    35041 ttgtatgata aggagtagac tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga
    35101 aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca cacgcgatct tagaacaacg
    35161 tggcgatgtc atggattttt ctgatttagc taaccagatc caaaactatc ttgggaaatc
    35221 agacagtgaa attcgtgatt cactcgcaca attttatact gacttgaata ttgacggcag
    35281 ctttatctca ttaggggata atcgttgggg cttgcgttca tggtatgcta tcgattcgat
    35341 cgatgaagaa gtaaaccatg gaatggacga agaggatgaa gatactccac gtcgccgtaa
    35401 acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt acaatgacga
    35461 tgacccagaa gatacagatc tgacagaaga agatgacgat gatctgtttg atgacgatga
    35521 tgatgaagat gaagaaatcg ctgcttataa ttctgacttg caagaaatcg gagcagatga
    35581 cccgacagat gatgaagaag acttgccagg aaatatcgaa gaagatttaa gtatcataga
    35641 cgaagacgat gatgatgaag attatgaaga ggaagaattt tctgactctg aaaaagaata
    35701 aacattattt tcttacgaga ggttgtgaca gagacgatca gcctatcaga ataaggaggt
    35761 gtgacacgat tttt
//
LOCUS       DAHHPA010000017        34776 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.018, whole genome shotgun sequence.
ACCESSION   DAHHPA010000017 DAHHPA010000000
VERSION     DAHHPA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.018"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            347..505
                     /locus_tag="NBH55_000782"
     CDS             347..505
                     /locus_tag="NBH55_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1508787.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            577..1803
                     /locus_tag="NBH55_000783"
     CDS             577..1803
                     /locus_tag="NBH55_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1508788.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(1891..2283)
                     /gene="rpsI"
                     /locus_tag="NBH55_000784"
     CDS             complement(1891..2283)
                     /gene="rpsI"
                     /locus_tag="NBH55_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCD1508789.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2299..2742)
                     /gene="rplM"
                     /locus_tag="NBH55_000785"
     CDS             complement(2299..2742)
                     /gene="rplM"
                     /locus_tag="NBH55_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCD1508790.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3261..3728)
                     /locus_tag="NBH55_000786"
     CDS             complement(3261..3728)
                     /locus_tag="NBH55_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCD1508791.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3848..5386)
                     /locus_tag="NBH55_000787"
     CDS             complement(3848..5386)
                     /locus_tag="NBH55_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCD1508792.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5466..6152
                     /locus_tag="NBH55_000788"
     CDS             5466..6152
                     /locus_tag="NBH55_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCD1508793.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6353..6880)
                     /gene="msrA"
                     /locus_tag="NBH55_000789"
     CDS             complement(6353..6880)
                     /gene="msrA"
                     /locus_tag="NBH55_000789"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1508794.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6964..7641)
                     /gene="pgmB"
                     /locus_tag="NBH55_000790"
     CDS             complement(6964..7641)
                     /gene="pgmB"
                     /locus_tag="NBH55_000790"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCD1508795.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7638..8768)
                     /locus_tag="NBH55_000791"
     CDS             complement(7638..8768)
                     /locus_tag="NBH55_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD1508796.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8768..10951)
                     /locus_tag="NBH55_000792"
     CDS             complement(8768..10951)
                     /locus_tag="NBH55_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCD1508797.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10948..11973)
                     /locus_tag="NBH55_000793"
     CDS             complement(10948..11973)
                     /locus_tag="NBH55_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1508798.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12018..12845)
                     /locus_tag="NBH55_000794"
     CDS             complement(12018..12845)
                     /locus_tag="NBH55_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD1508799.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12842..13909)
                     /locus_tag="NBH55_000795"
     CDS             complement(12842..13909)
                     /locus_tag="NBH55_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCD1508800.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13935..14771)
                     /locus_tag="NBH55_000796"
     CDS             complement(13935..14771)
                     /locus_tag="NBH55_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD1508801.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14768..15637)
                     /locus_tag="NBH55_000797"
     CDS             complement(14768..15637)
                     /locus_tag="NBH55_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1508802.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15707..16990)
                     /locus_tag="NBH55_000798"
     CDS             complement(15707..16990)
                     /locus_tag="NBH55_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCD1508803.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17004..18689)
                     /locus_tag="NBH55_000799"
     CDS             complement(17004..18689)
                     /locus_tag="NBH55_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCD1508804.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18964..19989
                     /locus_tag="NBH55_000800"
     CDS             18964..19989
                     /locus_tag="NBH55_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1508805.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20584..24258)
                     /gene="rpoC"
                     /locus_tag="NBH55_000801"
     CDS             complement(20584..24258)
                     /gene="rpoC"
                     /locus_tag="NBH55_000801"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCD1508806.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24316..27933)
                     /gene="rpoB"
                     /locus_tag="NBH55_000802"
     CDS             complement(24316..27933)
                     /gene="rpoB"
                     /locus_tag="NBH55_000802"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCD1508807.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28661..29629
                     /locus_tag="NBH55_000803"
     CDS             28661..29629
                     /locus_tag="NBH55_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1508808.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29647..30552
                     /locus_tag="NBH55_000804"
     CDS             29647..30552
                     /locus_tag="NBH55_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508809.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30549..31355
                     /locus_tag="NBH55_000805"
     CDS             30549..31355
                     /locus_tag="NBH55_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1508810.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31491..32480
                     /locus_tag="NBH55_000806"
     CDS             31491..32480
                     /locus_tag="NBH55_000806"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCD1508811.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32797..33321)
                     /locus_tag="NBH55_000807"
     CDS             complement(32797..33321)
                     /locus_tag="NBH55_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCD1508812.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33608..>34776
                     /gene="scm"
                     /locus_tag="NBH55_000808"
     CDS             33608..>34776
                     /gene="scm"
                     /locus_tag="NBH55_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCD1508813.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDST"
BASE COUNT         9960 a         7365 c         6503 g        10948 t
ORIGIN      
        1 aacttttttc gtagtgtgtc tttttataca tgtttatcgt tattaatagt gaataggaaa
       61 aaaactattt ttgatcattg acaattaaat acaatttttg aataacgatc cctgctattc
      121 gagctagaaa ctcaagtacg ccatgacctg attagcgaca aatacttaga gatttctttt
      181 tgttctggga aaacaaaatg ccatgacaat agcagcaaat aatgatactt ccgtaaggct
      241 cgccgtaaac tggggagagg tgaaattcct atgtgcttac ccaaagcaat tcaaaaattt
      301 cataactact attttaggag atgattttat agaatccaga gtctacctga ataaaaaaca
      361 aacctgtgca tttctaggaa tcgataataa tactctagat aagtggattc gtatatataa
      421 tttcccagta ataagagttg gtggtgtagt tcgctttgac gtagaagata ttaaagactt
      481 tatgaataca tttaaaataa cttgaaattt tcttctcgcc gcatatacta aaattagtga
      541 aatatactgc cttacttttt ttaaggagtg aggattttgc ctacaaagct ttcaaatggg
      601 aaatataaaa ctaatctgag atatccaaaa aggtttaaag aaataacagg aattgcatca
      661 gaaaagtatc aaaaaacatt tccaaatagg caattggcga ttaaagccga aaatgatatg
      721 aagaagaaaa tagaaaaagt tttgagagag gagaatgcaa actcattaga attaaaagga
      781 aaaataacgt ttaaaaaatt ttacgaatct aaatggcttc ctagatatga attaggtcaa
      841 acaattagat caaacaggcc tcctagtgat ataaccatta gtaatacaaa agatatattt
      901 cggttgcata ttttacctat gtttggtgaa tacgcaatga attatctaaa tatcaatact
      961 gagataatat cagatgaatt aaccaaaaaa tcgaaagaat atgcaaatat aaaaatcata
     1021 aaaggatatg ttcgttccat gttcgatatt gcagaaatac taaattatat cgaatttaat
     1081 cgaactacta aaattattca aagtatcact gcacctaaaa aaaatgcttt agaagaaaaa
     1141 agaatacaag aaggtaagca agcactatca tcaaaagaac ttactaattg gatagaagca
     1201 gtaaatgatg attttaataa tcatttactt acttttcatg attacacact ttttatgatt
     1261 accctgtatc ttggtgatag aaaaagtgag acttacgctt tgcaatggaa atatattgat
     1321 tttgaaaaac aaacggtccg tcttaaacat actttagata aatatcaaag aaaaaagttt
     1381 acaaaaggtc gaaaagatac cgtcattcaa gtaccagaag ttgtaatgac attgctaagt
     1441 gaatggaaat cggtacaagc tgatcaattg ttgaaattaa aaattaagca aacgttagat
     1501 cagtatctct ttacttatac taaaccatct ggagaagtaa attgtcctgt tcatgccgat
     1561 tacctaaact atcgaatcaa ttcaattaaa agaaggcatc ctgacttagt tcatttatct
     1621 ccacacaaac tccgtcatac ttatgcaact atagctagac aaggtggagc agatatgaat
     1681 caaatttcaa atgctttaac acactcagat atttctacaa caaagatata tgtgaacaca
     1741 ccagatatag ttgataaggc tgtttttgaa gcttttcaga gaggattgaa taagtgtgat
     1801 tgatatgtgt tcttaaatgt gttcttaaaa acaaaagaag cactttccaa ataattgaaa
     1861 agtgccagaa acgttgatat ataaaggata ttaacgtttt gagaattgtg aagctttacg
     1921 tgctttctta agacctggtt ttttacgttc gaccatacgt gcgtcacgag taagtaagcc
     1981 agcacgttta agagctaaac ggaagtcagg atcaacttct agcaatgcac gagcgatacc
     2041 atgacggata gctcctgatt gtccagcgta tcctccgccg tttacgttaa caaatacgtc
     2101 atatgcgcct tttgtttcag taacagcgaa aggttgattg attacttcac gcaagtcagc
     2161 atgtggaatg tattcttcaa catctttttt atttacagta attttgccag ttcctggtac
     2221 tagacgtacg cgagcaactg cgtttttacg acggcctgtg ccgatatatt gaacttgtgc
     2281 caatgaaatt ccctcctatt agattaagtt tgtgatgtct aaaacttctg gttgttgtgc
     2341 tgcatgtgga tgttctgctc caccgtatac gttcaatttt gtaaattgtt tgcggcctaa
     2401 agtgttttta ggaagcatac cttttacaga agtttcgatc aaacgacgag aatttttagc
     2461 acgcaattcg ccagcagtga ttgatttcaa tcctcctggg tagttgctgt gacggtaata
     2521 aattttgtca gtcgcttttt tacctgttaa tttcactttg tctgcattga tgacgatgac
     2581 gaaatcgcca gtatccaaat gaggtgtgaa agttggttta tttttaccac gtagtacaga
     2641 tgcaacaact gttgaaagac gtcccaatgg aacatcagtt gcgtctacta cataccattt
     2701 acgttctact tcgcctggtt tggccatata tgttgtacgc acgttatttt cctccaattt
     2761 aagttctgat gtttgacata cacttcgagt ttccggggct ctttgtgggg caaacaatac
     2821 catttaacat attacccgct aatctagtca atgtcaacgt ttttctgctt gattgaaaaa
     2881 tttatatgaa actttacttc ttttctttct tttatataaa agggtcgttt cctgtcaaaa
     2941 tgaaaaacaa aaagaggctg tgacaaaagt cttgtcacaa ccccttattt tgaataaacg
     3001 gtgttctcct gccaactcct cggtcacaag tcacaatatc cataaaacat aagaagctgt
     3061 tttttaaaat tgtttcttgt gactcggagc tgaacggcag gcttacaacc tcttggtaaa
     3121 cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta
     3181 ttttgacgtt tgtttctcta atactcaggt caaaacgctg atttcacaac ctctttttcg
     3241 gtcaagttca acaacagttt ttacgcgtcg atctctggcg ctttgcccaa atgagcttgg
     3301 atcatccaga tacgtttttc tgtggctgtt ttgaattcga taaagatatc ttgtgttgga
     3361 tcgtcgcctt cttctccaga aacttcgatt cctttttggt aaagatctgc tagataacga
     3421 tagcctgcaa caagtgtttc caaacgttct ggaatagtac gatcccagcg gccaatttca
     3481 tcagggattt ttgtgttatc tgcaaattca cgcaatgtag aataaggtga accgtctaat
     3541 gtgatcaagc gttcggatac ttcatctaac tgagcgttca aatcatccat gaactcatcc
     3601 atcattgggt gcaatgtaag gaaacctggt cctctcatgt accaatgtgt ttggtgaaca
     3661 acaacagaca tttggcttag atctgcaact aattgattta atacttcttt ggtttgttgg
     3721 aatttcatct ttcttgcctc ctaacatttt cttacactta aaataatagt agactttcaa
     3781 tgaaaagtac aacaaaatca tttacctgag aacgattttc agttcgcttt tttatccgta
     3841 aagccaatca tttttcagaa tcagctaatt cttctcgcag ttgagcaaat tgctgataag
     3901 tctgtgctaa atgcttttgt gtcgaaacaa ataatggata gattttctga tattttttat
     3961 gatcagtttc ttgtactcca acttgctctt tcaattctat caattcctgt gcttcactga
     4021 tactttggat ctttccgatg cttctccagc ctaaaatcac agctcctaat gcggatgctt
     4081 ctgtgacagt agggaaaaca agcgggtaac ccagtacatc agccatcatc tgacgaaaaa
     4141 tcggagattg agcaaacccg cctgtagcac ggatctctgt gagctgattt tcctggcaaa
     4201 ctccttttaa aatccgataa aggttgagac agatcccttc tagaatcgcc ttcatcattt
     4261 ctttttctgt atgttgccgc tgtaaaccga taaaactgcc aaacgcatct gcgtcccaaa
     4321 gcggtgctcg ctcaccaagt aaatagggat gaaaaatcag accattggca cccgctggca
     4381 caaaagcgat agccgaaagc agtgcatcat atggattttc tcctttttct ttagcagatt
     4441 gaacgacttc ctgtaaaaat gtatgccctg cccaatcaaa gatgccagca ccattgctag
     4501 tcgctcctcc cactacccaa tgtttctgat ccagaacata acaaaacgtc tccatttctg
     4561 gatggaactg aggctgagta gtaataaaac gaagtgctcc gctcgttcct acggtcaaag
     4621 cggcgatcgt atcaccaaaa gcacctaacc ctagattaga aagcggcccg tctgcccctc
     4681 ccaaaacaaa tgatatatca tttggcaaac ctagttctgc tgctgttttt gatggtagac
     4741 ctgttacttg ataggtggaa ggaaagactt gcggcaattg ctctattcga atgttcaagt
     4801 agtcaagcac ttcttctgcc cattggaatt catggatatc aaaatatcca gttcctgaag
     4861 ctgttccgta gtcgctgatc agttcgccaa acaatctata aaagatatag tctttgatac
     4921 cgacaaaata agcagttttt ttgaataatt caggttggct gttttgcagc cacttgattt
     4981 tgttcaatgg tgtcattgga tgaatcggcg tcccagtctg ccgataaaat tctttgccaa
     5041 gcacggtata tttcagtttt tctgcttcgt ccgctgctcg gttatctgcc caagtgatca
     5101 ttcgagataa cggtgttttc tcttcatcca acaatatcag gccatgcatc gcagaggaga
     5161 aggagacaaa ttgaatgatt gtgtctgaag gcaactgttt aattacttct tttataacgg
     5221 tgcagacacc ttgaaagatc aattcctgat cttgttctgc catcccttgt gtttcttgga
     5281 tcaaagggta gcgaaaataa gcggatgctg cttcttgggc ttcatctgtg aaagcaactg
     5341 ctttggtttg tgtggttccg atatctaatc cgattgaaac gttcattttt tcacctcaaa
     5401 ttttatgtat gtacaaaaat ttagtttaag cttgcgtatc ttggttttga aaagggggag
     5461 atggcgtgtt actttatttt atcattggtt gctgtatcgg atcattttta tgtttaaccg
     5521 cccagcggat tccgttaggt cactctatta tttatcctag atctcattgt gtcaaatgct
     5581 gtcgttcgct ttcttggtat gaattgattc cgattctctc gatcatcgtc caacgatttc
     5641 gctgccgcta ttgcaggtgt cggttgccct tctactattt gttggcagaa gccttatgtg
     5701 gtggtctttt tgcctggttc tttacttttt cttccgcaag aaatttttcg acgttcatat
     5761 ggatgctttc ggctttgcta ttttcgttga tggatttatt ttacttcatg gtccattctc
     5821 acacgctatt ctgttcttgg gcaattttat ggattttttg gttgcaggcc ggcgtcttcc
     5881 aatggcaaag tgtgctgtta acgtttatcg taggcggtgt gtgccttcgc tatggtcaat
     5941 cctatctcgg agctggcgat actctgttga tgctttcttg gagtggtggc ttgtctctag
     6001 aagagttgct tcagatcttg tttcttgcta gtctgtttgg aatatccgtt ttttctgttt
     6061 actttatctg tcataaaaaa aagttggaaa agctgccttt cattcctttt ttgagcagtg
     6121 cgttgctgat tgtccttcat cttaggtatt gaaaaaaggt tgtgacaaac gacttgtcac
     6181 aacctctgat atatagtgtt ctcttaccaa atccttgtga cttggagcta ggcgacaggt
     6241 tcacaacctc tccactaaat ggtgttcaat cagctgaccc tcagtattag ctcaaaatgc
     6301 tggttttaca acctctgata tacggtgttc cataagtagc tttttcggct tcttatttct
     6361 tgacgcccca atgtcgacga aggaaacctg cacgtccaga accttctcga taaagattgt
     6421 aatggagctt gtttttcttg taataatcct gatggtaatc ttctgcagga taaaattcgc
     6481 ttcttggttc gatttttgtc acgattggct tatcgaatcg cccgctgtct tgcaatgctt
     6541 gttttgaacg ctctgcaata tttcgttctt cttcatcatt atagtagatc actggacgat
     6601 aagaatcacc gcgatcagcg aactgtccta atgcgtcagt cggatcggtt tgctgccaat
     6661 agatttccac caactgatca taagaaataa cttctggatc atattcgatc tctactgctt
     6721 ctgtatgccc tgttgtgcct gttgttactt gttcataagt tggatttggt acatgcccgc
     6781 cagtataacc ggatgtcaca gaaatgatcc ctggctgctc ttcaaatggt ttgaccatac
     6841 accaaaagca tccgcctgcg aaaactgctt tttttgtcat ataaaatccc ccttttttct
     6901 catcatacct tgatttccaa aaggaggata acgttttgct tgtgacagaa aaaacggaaa
     6961 ctattatata cgtgatacgg cttctgataa atgccaaaac gaacgtactt gatcaaaaac
     7021 ggttaaatca ggaatcacat attcagcaga aggaagattt atgttttcac ctattcccaa
     7081 tacatgcatg ttaccagcca aagctgcttg acaaccagct tctgaatctt caatgaccaa
     7141 gcatgcattt ggcggtacat tcaattgctg tgctcctttg agaaaaactt ctggatcagg
     7201 ttttgctttt gatacttgcg tgccgtcaat aaggacatca aaataacttg tcaaattcgt
     7261 tttttctaaa atgaggcgtg catttttgct tgctgatccc aatccgatct tgatgtttgt
     7321 ttttttcaag tactcaagaa cagctattgc accaggcaaa acggatgttt catccatttt
     7381 ttgaatcgct tgaagatata gattgttttt ttgctcagca aaggctgatt tctctttttc
     7441 tgagaatttc tgttccattt ttccaaatga cagcaatcgc tctagtgact cggttctgga
     7501 tattcctttc agttgctcgt tttctttctc agtgaatgga atggacagct ggttagctaa
     7561 aaccagccac gcttcgtaat gataacgagc tgtgtctaca agaacgccat ctaaatcgaa
     7621 tagtgctgct ttgattttca tcgattacct ccttgaaaat gaacgacttg aagtaaagat
     7681 gcgatgtttt tagctgtgcg atgaagatta tcagtggctt gctgtaaagc ttcttgtaaa
     7741 ggcacagctg caggaaggat cggaaaaacg gccgaaatgc caacatctgc tgctttttcg
     7801 cttcctttac caacacttcc gcaaatagct atcacaggaa cgccctctgg tactcttcta
     7861 gcaatcccaa ctggcgcttt tccttttaga ctttgtccat ctagacgtcc ttcgcctacg
     7921 attacgaggt ctgcttgctg tacccgttta tcgaactcca gtaaatccaa tacgtagtcg
     7981 atgccagaga ctattttccc tgaagcaaat gctaacagtc ctgcacccat tccaccacca
     8041 gcactactgc ccgatgcctc gattacttga ggggcaaatc tcatataaaa atcatgcatg
     8101 gcttggtcag ccactgctaa ttcattttct ttcaagcctt tttgtggtcc gaaaacaaaa
     8161 gaagcgcctt cttttccgca taaaagatta gtaacatcag taatgacagt gattttggct
     8221 ttaatcagct caataggcac gttcgtttta tcaactgtag caattttacc tagattttct
     8281 cccagtgctt ctactttttg attatcttga tcgtaaaaag cataacctag accttctgcc
     8341 atgccgatgc cgccatcgtt ggtactactt ccgccaacgc cgatgatgat gtgtcgaaca
     8401 ccttgttgca acaattgtcg tatcagttct cctactcctc gcgtggataa cctaagtggc
     8461 tgttgttggt ttagtgggac atctttcaat ccgacgattt ctgccatttc aaaaatcgca
     8521 gtttgaccgt ctgaagcgta ctggatttct ttgtttttgc caaatgcatt agtaacgact
     8581 gctgtttgcc gcttcaacct taaactgtct gctaatgatg aaaccgttcc ttctcctcca
     8641 tcgcctatag gtagcagatc atattcagcc tgaacaaaaa cttcttgaaa acctgattga
     8701 atggcttgtg ctgcctctaa ggctgtcaga ctttctttga aagaatctgg cgcaatcaga
     8761 atacgcatta ttttcccctc cgttccaaga tcagttcttt ttccagctgg tattggtctc
     8821 cattgacaaa aataggtaat gtttctttcg tctctttttt tattactatt tgtttttttg
     8881 tcaaaacaaa tagcaacctg tgatttttcc aaataaaagg aaaagaaatt ttttcccatg
     8941 ctgaaggaag tcttggagaa atagaaagag ttccattcgt tactgtaata cctgaaaaac
     9001 caaatataac tgctagccat agtgctccta atgaagcagc atgaattccg tcatctgatg
     9061 aatgcggatc tggtcctaaa tcaatcaggc acgcttcttg gaataaatga tacgcccatt
     9121 ccatttcttg gatacggcaa gcctctatgg cgtgaatcgc tttactcaac gatgagtcgt
     9181 ggattgtttt gttttcatag taatgcaaat tcttagaaac gattggttga gaaaaatact
     9241 caggtaataa gtataacagc atgacaagat ctgcttgctt gctgacttgg agttcattta
     9301 cttcgctacg ggcataatca agtaaaatag actggctgcc aggacgttct ttgtaagtgg
     9361 tcaaatcaat aactggctta ctgagatacg tgtcatcttg cggaatcagc tgttctttgt
     9421 taggcactgg caaatataac ttatctaaaa aattttgaca ttctttcaaa aaaagcggat
     9481 cgccagcttg atatttaaca gctaaggcca cattataatg cgccagatag ttcgtatatg
     9541 cattattgtc gatatattct gtgtattcat ctggaccaat gactctgtgt atctctaaat
     9601 gatcatcgac ttttacagct ctgctcagcc aaaaagctgc tgtttcatga atgagcgttt
     9661 ttccctttgt ttctaaaaac gattgatcct ctgttgcttg gacatactct tgaacggcat
     9721 aagcaaggtc tgcactgata tgatgttctg cggcagctga agcaacttct tgtcttgttc
     9781 cggttttgat attcaatgca gcaaatttgg gtgtctcttc ctgaccagtg tgtgcgcttt
     9841 cccatgggaa aagtgcacct tgatagtgat aatctcttgc tttttgccgt gcgccttcta
     9901 atcgatcata acggtacagc agcaaattct ttgctacttc aggaaactga tataaaaaga
     9961 aaggcaggat aaaaatttcc gtatcccaga aaacatgccc tttataacct tcgccagtta
    10021 gtcccttcgc ccctacacta acacttggat cattgcgggg agtcataatc tgtaaatggt
    10081 aagcagcgaa gtccatcgct aactgatcga aagaagaatc gccttggatc gttatccgat
    10141 gctgatgcca aaaatctgtc caataagaca tcgactgctc taaaatttct gtataactaa
    10201 cgcttttctg ggcttgggca atacttttct taaacgggtc tgtttgctga agagaggtag
    10261 agagcacgat gatcttctcc agcgtaaagg acgtattttt tctgatcttt tctgaaaagg
    10321 aagcggtgat ccggcgattt tttgcttgga atacacctgc tttattgaat ttagctgcaa
    10381 tcccaatatc aattttactt tctgttgtct ggtaggatgc actgatcaaa tcgtcaccaa
    10441 atactcgcag ttctttttca accaactgct gtgtaccaaa attggtttgc tgtccatcga
    10501 tacccgtttt cacatctact tggcagtctt cggtcaacgg cttgatgacc actttcaggg
    10561 ctgcctgatg gagattattt tgatcagcaa cacgacaagt aatgacttcc actttttttc
    10621 cttgagcagt ttcaatgagc gcatagcgtt taagctctcc catttctaaa tctagtgccc
    10681 gctcgtatgc aagaactttc atcgtctcaa gagaaaaatg ttcaccgtcg atttttattt
    10741 cccatctgat gacatctggg agattcacta actcggctga ttctgctcct aaaggtcggt
    10801 tatatacacc agctgcaaac attcccctta cttgattccg atagtcttcc tcctggcttc
    10861 ctctgatacc aaaaaatcca tttccaatgg tcatctgact cgcatactta ttgatatact
    10921 ctaaatcaaa ctgtttttct tctactttca taaggtaata acttctcctg tttctgccga
    10981 acgatataaa ttttccacca gattctggat gacatatcct tgccagccat ctgcaatcat
    11041 ctgagtctgg tcaccgagcg cccgattcac aaaatgagcc atacttagtt catgtgggtc
    11101 attaactggc gtggtctttt gtaaggaaac cagctttcca tctacatctt cgaatagttc
    11161 gcctggatac agattagcac ctgcctgatc gccacaaaaa gccacattca gtttgtcttc
    11221 tctgatattc aaagcaaacg aagtattcag ttggatgatc ttgccgtttt ctaattccag
    11281 tgtgccaaaa attgcatctt ctactgtgaa tttgcttgga tcccatttcc cgaatttccc
    11341 tgaatttttt ttcgtaccaa ttttttggaa gctgtacgcc cgaacgcttt ttactttagg
    11401 aaatccagta atatacatcg ctgtatcaag catgtgaatg ccataatcga tcaaaggccc
    11461 gcctccttga agttctttgt cgataaacgt tccccagcct ggaacaccag accgacgaag
    11521 cgcattcgct tctacgtaat aaatatcccc gagaacatca agatgattct tgatcattgt
    11581 tgcttcgtca gaaaaacgat gctggaatcc ataagccaaa atctgcccgt ttttatcggc
    11641 tgtctctgcc atctttcttg cttgatcagc tgtcatagct ggtggtttct cgcacatcac
    11701 tgaagcatta gcttcgaggg cagccaatac atgttcgaag tgaaagcgat tgggtgtaca
    11761 gacactcacg atatcgggag aaatctcatt cagcatttgt gtaactgatg tatacgcatg
    11821 agaaaatcct gtacgtcggc aaaagtcttc tgctcgctgg ctgactggat caaccacagc
    11881 ggccaattct aagacatctg atcttttttg ataataagca gcatgtactt tttctgctac
    11941 ttgtccagcg cctatgattg ctactttctt catttatttc tccctccttt gcttatttac
    12001 ttattgttat tctttctcta ttctttctct aaatcattta aatgacgcgc tgtttccaga
    12061 cagtttttca agtattgcaa tgattcctga taaattttca cttcattttc tcctcgcaaa
    12121 cgacattcat ataccatata accttcatac tcattttgaa gtagcgtttg aaaatggcga
    12181 gtaaaatcaa ttgcgccaga tcctggttgg tagcgatgat tatctgctaa atggatatga
    12241 cccacatact ctttttgcgt agtcaatgct tttgaaatat catcttcttc aatattcata
    12301 tgataaaaat cagcgatgat ctgcacatgt ttcaattgat tagagtcaat gtaatgacgc
    12361 gcatccgcta acaaattgat catatggtct tggtatcggt tcaaaggctc caaataaata
    12421 accgtacctg tttcttcggc tactttatct aaataacgaa gtgattcgct gactgcttta
    12481 tagtctcctt ctttgcttcg gggtgaaacc atcggcggta atcgataagt gaacattccc
    12541 caagctgctg ggataacgat tcctttgcct ccaacctcgc ttagtgcacg caaaatctca
    12601 gcaatttgac gtacaccatt aagacgccgt tcttcgataa agtcaccgat ccacccatcg
    12661 tagccgttac aagctgtcac tactgggatc cctgtttctt tgattgcctg tttgactgtg
    12721 tcgatatttt ctattaacaa cttaccgtcg atttcgtatt cttcaaatcc acagtctttg
    12781 ataaatctaa atttttctaa aatgtctctt gggaaaaatg cttgatcttg cgtaccgaat
    12841 ttcattgttc tcctcctcgg acttgaacgc ccattttgat actttcttct gggtgctggt
    12901 ctacataacg catgtaagct tctgcgcttt ctgcaaatgg cacaactgga tcaatgattt
    12961 cttcacaatc caagtaaccg ttcatcaata attcccaaca tgtgtcctcg attcgtttac
    13021 ggttccatct tgggtaatct gggtttggtt cactagatgc tcgagcaaag acgatttttg
    13081 cattattgaa atgtgcttcg cgaccaagat taaacccagc tggaaaaggt ttagcaaagg
    13141 caacataaga aatgatcccg ccatagccga tccctcttaa agaagcttgt aatgcggcag
    13201 cactgccact cgtttcaata actgaatcta ctccacgttt atctgtcaat tcctttagac
    13261 gaaaaccgac atctgctttc ataggatcta gcgtgtaatc ggcaccgtat ttttctgcta
    13321 attctctacg ctgcgctatg ggatcgatcc caacgatggt agtagcacct gcttttttag
    13381 ctaattgaat cgcaatttgc ccgatagcgc ctaaaccgaa gattgctaca gcatcacctg
    13441 ctcgaacatg tgcttcccgg ataccaccta aagcgaattg agcaggatcg taacatactg
    13501 catttttcca ttgatccaac cgatcaagtt tacgtagttt ataattatca acaccgtttg
    13561 taatcagcgt ttcacgaatg ggtccataag tgcagactaa ctctcctttt tgatactctg
    13621 tcaccaaact gcccgtttct tcgatcgtac cgacaatcat atttcctaat tgaaattcgc
    13681 caaataccac accgcgttgg cttccttctt ctcttggcaa gaataggcgc cattcttctg
    13741 aaaattcttc atcgataaat ggacttaatc ctctaaagtc gacaatttct gttcctggtt
    13801 ttggagtagc gaaaattact cggatacgta cttcgttctt ttcgatctca cggtcttcgt
    13861 aatctttgaa tccggctatt cttggttcta atgcaactaa ttttttcatc ttttctttcc
    13921 tctttctttt ctttttatcc ttttacgccg ccttcgacta aatcaccttt aataaatcgt
    13981 tcagataacg catacataat gatgactggt aaagcggtca ccaatgaagc ggccatcatt
    14041 cgtccccaaa tgtagtctgg tgtactgaat aatgtgttca aaccgattgg taaagtaaat
    14101 ttttcggagg tcgacaggaa aatcgaagca aataaataat cattccacga aaccataaag
    14161 caataaacga agacagaaat gattccagaa atagccaatg gtataatgat ccgaataatg
    14221 atctggatcc ggctaagacc atcgatcatt gctgcttctt caatatcagt cggaatcgtg
    14281 tcaaaataac tttttagcat gaatatcgca gtaggaagtg tttgaacgac catcgtgata
    14341 atcagtgctg tattggtatc atacaaacct agaccggaaa tgattttaaa taatggaaca
    14401 acgatcaaaa taccggaaaa catataaacg gtgtaaaagc tagcattgat tttcgagcga
    14461 gctttgaagc gtagttttga caaagcgtat gccccgctga tcccaatcac tactgcagtt
    14521 acggaggcta tcaaggagat cactaaacta tttttgaaat acatgatgaa tggaaaaatc
    14581 tcagcattga aaatatcctg ataatgttca aaagtccatt ctttcggaaa aatagtttgt
    14641 gtcgttgcaa ttgcttcttg tgaatccttg aatgatgtca tcagcatgat caaaaatgga
    14701 aacaaattga cagctaatag caacaaaatc gtcgcgtaaa ataagatttt cctgctcttt
    14761 ttatttttca tttcaagtcc accttctttc gtgagagcaa gacgaccgct gcaataatga
    14821 tgaacaatag aatcgatata gctgctgctt ttccaagatc attgaacgca aaggctgtct
    14881 gatacaaata aacgcctaag atattgactt tattcgtcag tagatagaca tctgtaaaca
    14941 tatagaacat ccagatagat cgtaaaacaa tcacagttgc tagtactggt ttgattgccg
    15001 gcagtgtcac gatcttgaac ttctgccaag catttgcacc gtccatttca gctgcttcat
    15061 aaagcgtttt gtcgactgtt tgcaaaatag ccaaaaatga aataaacgca tacggatagt
    15121 atctccagat agcaaacaaa acgaccataa agaagctagc aactggctgg tcgaaccaca
    15181 gcggtgcttc gttgaaaagc tgcaacctat ctactaacag atcattgacg attccataac
    15241 tattattgaa catatatttc caagcaaaaa tcaaagaaat agatggcgta acataactta
    15301 ataaaatcag tgaacgcacc gttttcctaa atctgaactt gcggttaagc aaaatagcta
    15361 cggctaatcc taaagctgta ctgcctgcta caactaagaa tgtgtaagca actgttaaac
    15421 ctaaagaacg ataaaattca ctatcactta aaatttcaag ataattgctt aatccgataa
    15481 aaacggcgtc tgagttagga ttcagcggca tatcgaaaaa actgatctga atattggaaa
    15541 gcattggaat cagcactaaa acagctaata aaagcatgct tggtgctaaa agtcccatgg
    15601 ctaattttaa gtcagaatgt ttccttggac gattcattga aacctccccc tgataatctg
    15661 ccaatagcta tctacttttt tcgaacacca ttggcagaac ttcttgttat ttcgtatttt
    15721 tttgtgcgtc agctaaagct tgatcgacat cttcgtttcc gacagttact tggttcactg
    15781 cttttccgat cacacctgag cttgtgatat ctcccatttt cgtgaaattc ttttccccga
    15841 ctagtccaaa gacttgtact tcatcaaaag cactagcaat ctcagatggc agctcgccaa
    15901 aagacttgat gacttcattt tctttgtaac catccagttc gactacttgt ttattcactg
    15961 gttgagcacc accaggagac atcaagaccc acttttccat gtttttcggt tgagataaga
    16021 aagctgtgaa tgcttcagca gcctcttttt gtttctcgtc tagaccagaa gaaatcgtca
    16081 atccagatac tgtgccatag acagcttgat ctttttgtgt tgggatagca aaaccgatat
    16141 tttctgagtt ccccgcttca taaacagaag gcaaaatgta agttgaatag attgccattg
    16201 gtgctgttcc attcatgaaa gcatctttga tttctgtcac atcattagag cctggcatcg
    16261 tgtattggga taagttttga taaaacgaca atgcttctct catcttttcc gtatcgatcg
    16321 ttacctcgcc ttcatcatcc aaaacatttg ctccatttga caaagcaaat tgagagaacg
    16381 cttgctcact cattgttcct tcagcagtag gcatagcaat tccatattgt ttattctctt
    16441 tatctgtgaa atgttgagca atttttaaaa tatcatccca attttctggt tctgaaaaac
    16501 cagcttctga taatttttct ttgtcatacc aaataccttg aacccagccg ctaattggtg
    16561 cagcaatata gctttttcca tcttctgaac gaacaaggtt tttggctccg tcataataat
    16621 tatcttctcc agcttcctcg ataacgtttt ggactgccgt ctgatcaatc agttcatctt
    16681 tatccatcac tttagcaaaa tcctgactga cttccattac ttctggtaat ttaccggaac
    16741 gagctaacgt aacgattttt gtattgaacg ctgattcttc taccggcact tgcttgattt
    16801 tgatgtctgg attttctttt tcaaaatcag caaccagttc attgatcaca cttaatcgtt
    16861 cctgttcgac agaagagtgc atgaattgaa tcgttgtctg ttgtgattcg ttagagcttt
    16921 gactttcacc acaagcagta agaagaaatc cagtagctag cagacaggta gtaacaataa
    16981 tttttttcat ttttttccac ctcttatttt attattttca gccacatata ttcatacggc
    17041 tggaatatca actttcgttc ttgactgcaa tggataattt gtccagaaaa caggtctagg
    17101 taatctcctt ttggtaaaag ataacctgct gattgattgg aaaaattatg gataactaag
    17161 actttctctt ccttcgcttc gcgaataaaa ctgataaaag aatctgctga gtccagtaag
    17221 taaatcaaac tattcggatg gaaggctttt tctttttttc gaatagatag catctgggtc
    17281 aattcaccat atactgctgt tcttaatgga tctgtctgca actctttctc aatatctgct
    17341 aacaaaaatt tttgccgatt gatttttcga ttctctcctg ttttttctac gcctttgtaa
    17401 tcatttcgac ttcccagcac actttgaata taaattgccg gcacgcctgg taacgttacc
    17461 aaaaatgaat gagcagctaa aaagcgtttg cttctccatt ccgtcgtatc tgttggagaa
    17521 cttaacgcat ctagatacgt agcgtttatc tcatatggac tggtcgttcc atctgggttt
    17581 ttcttgtagt ttactcgggt accctctttc ttcagctgtt ccactaattc aaggatcgtt
    17641 ttttccggca gaatacctcg taccggattc aatccgatac cgtcatggga ggctaaaaaa
    17701 ttaaaaaaag ttgttttttt cgatgcgaaa tcgatggttt ttaaccattc tttcaactta
    17761 cttgtattac ccgaacgaat cgcttgaagg gtcaaaggtg gtagcgaaaa ctgatagacc
    17821 atctgtgctt cattctcgcc attcccaaaa taactgatat tatctttgtg tggcacattt
    17881 gtctcagtga tcaataccgt tcctggagct gcttcatcag cgattgcacg gaataattgg
    17941 atgattttat gcgtttctgg caggtgtata caagaagttc ctagttcttt ccataaaaat
    18001 cccacagcgt ccaagcgaat aaatgaagcg cctttttcta agtaaaacaa aagaatatcg
    18061 atcattttca ataaaacttc tggattttta aaattcaagt ctacttgatc ttcgctaaaa
    18121 gtcgtccata cgtgtttttc tttcccgttt ttgtcaatga acgttgtaag taacgggctg
    18181 gtccttggtc tagtcacgct gcttgtatct gtatccagtg gcaaatcaat aaaaaaatct
    18241 ttgtacgtat cattcaagtt taaatattcc tgaaaccaag tactttcggc agacatatga
    18301 ttgcagacaa agtcgaacat taaccgtgtt tcgcttctca tttcttctat atcttcccat
    18361 gtgcccaaac ggtcgttgat tgcctgataa tttacaacgg agaaaccatc atcagatgta
    18421 tatgggaaaa atggcaagaa atgtacaact ctaaatgtat ctgccaaata cttttgataa
    18481 aatttcttaa atgtacgcaa agttggttgc tgctcttcaa aaaattgatc cccataagta
    18541 atcaagaaaa tatcttcttc gtcccaatgc agtttgcctg cctggccttt cttctgctga
    18601 taaacactga ctttttgttc aatcatagag gcggttttct cagacaaatc tgagtcgcga
    18661 taaatcgctt gtaaaagttt tgtatacatc ggattcccct ttcttttttg ggagcgttcc
    18721 caacgatttg atcatattat ggaaaacgct tactgtcaat agcatttttt taaataattt
    18781 tgatatacac aaaaatacaa ataagaaagc ttatgtatca ataattaata gcttcttaaa
    18841 aaaatatggt ctctcatgat catgcaagct attcctaaat tttcaaaatc atagacaaaa
    18901 aagaatgtct attgtactat ggaattattc taaaacgctc ccaaaaagga ggtgcatata
    18961 aaattgaaac ctactattta tgacattgcg aaaaaagcta atgtctcaaa atcaactgtc
    19021 tccagagtat taaataacca gccaaatatc tctgataaag caaaagcaag tgtacttcaa
    19081 gcaatcaaag aattgaatta tgaaccgagc aaaattgcaa gaggactgtc aggaaatggt
    19141 tttgacgcga ttctagcatt atctcatcgt acttctcata cgacctatgg caatcctttt
    19201 ttttcagatg ttattcaatc catttctgcc gtttctgaac aaaataactt cgacttgatt
    19261 ctacaaacgg caaaaacacc agaagaagag ctagaaaaat gcgcgaaaaa gatccaagaa
    19321 aaaatgatcc gaggaattat tattttaagc tctacgatgg atgaaacact tctttttgaa
    19381 ttggatcaat acaatattcc tattgtggta atcggaaaag tcggaaacga ctacacgaac
    19441 atctactcag tggacacgga taatttccag gatagttacg atttggtgga aacactgatc
    19501 aagaatggac accgtcatat cggctgtctc catgctccga cagaagttta tgtatccaat
    19561 gatcgtctca atggttaccg tcaatgctta tttgatcacc acctagatat ccgtaatgaa
    19621 tggattatag acggcggctt tcgcttagaa gatagcatgg cagcaatgaa tgatttaatg
    19681 aagctacgag aaagaccaac tgcgattttt tctaccgatg cattaaaatc attgagtatt
    19741 tacaaagtgg cagaacaatc tggaaagcag atacctgatg atctttcgat cgcaggattc
    19801 agtgatccct tattgtcctc cctattttcg ccacagttga ctcggataga cattccgaca
    19861 aatcagttag gaatcaaagc cacagaactt ttattccaac taatccacca ccaataccct
    19921 gaagaaaaaa gaatcattct ttcaaccgag attactctca cagattcgat ccaacaacaa
    19981 gaaatgtaga acagaattat aaaagaacag aaacactgag gataagtcag ttactctgtt
    20041 cttttttcaa tcactcatcc ctcgttaccc cctcccggta ttttagcgaa tttttcttat
    20101 tttccatctg ttttagatag ctgtggataa ctataatgtt tgtgttaaac tattttttcc
    20161 acagggaaga aaaaactatt tttaaccacg ttgtgtataa gtttttatag ttcgtttttt
    20221 taaacacatt tacccttatt tcaatccatg ttatttgggg atacttatct ctaaaacata
    20281 aatagaataa cagacatgtc tactggtttt gttttattcg aggaattatt caataatagc
    20341 tagcaaaata ttaaaaataa ccgctctatc ttcccatcaa tttattttgc attcaaaaaa
    20401 tgatctatct ttacttttgt tgattgactt ctcttggaag atgatcgttc cgatacctta
    20461 attggatacg aaccgatgtt gttaagatcc tttcccactt ttcttctagt tagacaaatt
    20521 attttagaca aaccaaaaaa gaggttgtga caataatttg tcacaacctc ttttttaaaa
    20581 tatctatttt gtttgattgt tcattgcgtc ttctgcagcc atttgcgctt caatatctga
    20641 aatactgtag acattttcgc ttgctacacc gacttctttt ggttccatgt cacgataacg
    20701 ttgcatacca gtaccagctg ggatgatctt acctataata acattttctt tcaaaccaag
    20761 aagaggatct ttcttaccgc ggatcgcagc atccgtcaag acacgagttg tttcttggaa
    20821 tgatgcagca gataagaaac tatttgtttc taatgatgct tttgtgatac ctagtaagac
    20881 aggtcgagaa gttgcaggaa cgcctcctga gactagcgta ttgtagtttt gatctttaaa
    20941 gtctgcgatg tctaataatg tacctggtaa gatatctgta tcacctggat ccattacacg
    21001 aactttacgc agcatttgac gaaccattac ttcgatatgc ttgtcgccga tttctacccc
    21061 ttgcatacgg taaacacgtt gtacttcacg aagcaagtag ttttcaacag ataatacatc
    21121 gcgaacttgc aacaattgtt ttggatcgat tgatccttct gtcaatggtg caccacggtg
    21181 gatcatatcg ccttctgcta ctttcatacg tgctgtataa ggaacagtat acgtacgtgt
    21241 atctgttttt cctttgattg ttacttcttt ggtacgtgtt gcaggatctt ctgagatatc
    21301 gatgacatcc cctgttactt ctgtgatcac tgcttgccct ttcggattac gtgcttcaaa
    21361 gatttcttgg acacgaggaa gaccttgagt gatatcgtct ccggcaaccc cacctgtatg
    21421 gaatgtacgc atcgtcaact gagtaccagg ttcaccgatt gattgagcgg cgatcgtacc
    21481 aactgcttct ccgacttcaa cgtcagagcc agttgcaagg ttgcgtccgt aacaatgttt
    21541 gcagacacca tgttttgtat tacatgtgaa gacagaacgg attgtcactg tttcgacacc
    21601 tgcatcaacg atttgttttg cgatgtcttc ggaaatcaat tggtttcctg caatgatcaa
    21661 ttcatttgtt tcaggatgac gtacttcttt acgggtgtaa cgtcccacta aacgatcttc
    21721 taatgattcg atgatttcat ttccctcgcg gatggcttgg atttcaagac ctcggtcagt
    21781 accgcaatct tcttcacgga tgattacatc ttgggccacg tcaaccagac gacgagtcaa
    21841 gtaacctgaa tcggcagtct tcaaggctgt atcggtcatc cctttacgag ccccgtgagt
    21901 tgagataaac atttccaaga cggatagtcc ttcacggaag ttcgaaatga ttggcaattc
    21961 catgattcga ccattaggag cggccatcaa accacgcatt ccggcaagct gtgtaaagtt
    22021 ggaaatgtta ccacgagctc cagaatcaga catcatgaag attggatttt tcgcttccaa
    22081 accttccatc agtttttgtt ggatgctgtc ttttgcagca ttccaaacag caatgacacg
    22141 ttcataacgt tcgtcatctg tgatcagacc acgacggaat tgttttgtaa tagtttcaac
    22201 ttgtttatgg gcattttcaa tgatttcttg tttttcattc agtacactga tgtcagcgat
    22261 ccctactgtg ataccggcat gtgtcgaatg tttgtaccct aagtctttca tacggtcaag
    22321 catcttagaa gtttctgtta ctttgaatcg tttgaagact tctgcgatga tgtttcctaa
    22381 gtttttcttc ttgaacggta atactaattc ttgctctttg atatgtgcag gaatatcagt
    22441 accagcttca acaaagtatt tgtccggtgt ttggacggtc aagttgaagt ctgttggttc
    22501 attcagataa gggaattcag gaggcatgat ctcgttgaag atgatcttac caactgttgt
    22561 gatcatcgta cgttcttttt gccattcagt aaatggtttt tcacctaatg agttagggtt
    22621 cacaccgata cgtgaatgta aatgaacata tccgttgcgc catgcgataa cggcttcgtt
    22681 ttgatctctg aagaccattc cttcgccttc gcgtccttct tcttccattg tcaagtagta
    22741 gttacctaga accatatctt gggaaggagt aacaactggt ttaccatctt tagggttcaa
    22801 gatgttttga gcagctaaca tcaacatacg tgcttctgct tgtgcttctt cattcaatgg
    22861 aacgtggaca gccatttggt ctccatcgaa atcggcatta taagcttcac atacaagtgg
    22921 atgcaaacgg attgcgcgtc cttgtactaa aactggttcg aatgcttgga taccaagtcg
    22981 gtgaagcgta ggtgcccggt tcagcaatac tgggtgttcc ttgattacgt cttctaaaac
    23041 atcccatact tcgtcttctt gacgttcgat tttacgtttc gcatttttga tgtttgaagc
    23101 tagttcgcgt tgaacaagct cacgcatcac gaatggtttg aataattcaa tggccatttc
    23161 ttttggaagt ccacattggt acatcttcaa gaaaggtcca acaacgataa cggaacgacc
    23221 tgagtagtcg acacgtttac ctagcaagtt ttgacggaaa cgaccttgtt tccctttcaa
    23281 catatgagaa agagatttca atgggcggtt acctggtcca gtaactggac gtccgcgacg
    23341 accgttatcg atcaatgcat ctactgcttc ttgcagcata cgtttttcat tttgaacgat
    23401 gattccaggt gcattcaagt ctaacaaacg tttcaaacgg ttattacggt tgattacacg
    23461 acggtaaaga tcattcaagt cacttgttgc aaaacgtcca ccttctaatt gaaccattgg
    23521 gcgtaaatct ggtggaataa cagggataac gtccataacc atccagcttg gttgatttcc
    23581 tgaagcgcgg aatgcttcta aaatatccaa acggcggatt gcacgtgtac gtttttgtcc
    23641 ttgtgcagat ttcaattctt ctttcaattc ggctgcttct gcatctagat caacattatc
    23701 aagtaattgt ttaatcgctt ctgcacccat agcagcttgg aattcttgtc catattgttc
    23761 acgtttttcg cgatattcgc gttctgtcaa caattgtttt ttctctaaat tcgtatttcc
    23821 tggttcgatg actacatatg aagcaaaata aatgatttct tccaaagcgc gtgggctcat
    23881 gtctaataca agacccatac gtgaaggaat ccctttaaag taccagatat gtgaaacagg
    23941 agctgctaat tcaatatgtc ccatgcgttc acggcgaact ttcgaacgag taacttccac
    24001 tccgcatcga tcacaaacga ttcctttata acggatacgt ttgtattttc cgcaggcaca
    24061 ttcccagtct tttgtaggac caaaaatccg ctcacagaac aacccttcgc gttcaggttt
    24121 taaggtacga tagttaatcg tctcaggttt ttttacttca ccgtaagacc agcttctgat
    24181 tttttcggga gaagccaaac ctatttgcat actttcgaat ttatttacat cgatcaaaag
    24241 gggttccctc catttcattt aatggcaaac cgcaagacgg ctgatcttcg accgccttgc
    24301 agctcataga ccggattagt ctaaattatc ttctgcggtt tcaaagtttt ggatgacatc
    24361 gtctttttca tcttccactt gttcagcagc tttcttttct aattctttgg cagtttgctg
    24421 ttcggcaaat tttgtcaaag catcaacagt gatcaaatcg tcgtcttcat cgtccatgtc
    24481 gcgaagttcg atctctgttt cttcgatatc caagacacgc atgtccagac ctagtgattg
    24541 caattctttc accaatacgc ggaatgattc cggtacacct ggttttggaa ttggttcacc
    24601 cttgacgatt gcttcgtatg ttttcacacg tccgactacg tcatctgatt tgtatgtcaa
    24661 gatttcttgt aaagtataag ccgcaccgta agcttccagt gcccatactt ccatttcacc
    24721 aaaacgttgt ccaccaaatt gagctttacc tcccaatggt tgttgtgtaa ccaatgagta
    24781 aggtccgatt gaacgtgcat gcaatttgtc atcgaccatg tgagcaagtt tgatcatgta
    24841 catcacaccg acagacacac gtccatcaaa tggttcacct gtacgtccgt cgtacagaac
    24901 agttttcgca tcgctggcca taccggcttc acgaactgtt tcccatacgt cttcatcact
    24961 tgcaccatca aatactggcg ttgctacgtg gatacctaac tgacgtgcag ccatacccaa
    25021 gtgtaattcc aatacttgcc cgatgttcat acgtgaaggc acccctaaag gattcagcat
    25081 gatatcgatt ggtgttccat caggtaggaa tggcatatct tcttccggca taatacggga
    25141 tacaacccct ttatttccgt gacgaccggc cattttatcc ccttcatgga ttttacgttt
    25201 ttgaacgata taaacacgaa ctaacatgtt tacgcctggt gacaattcat ctccagcttc
    25261 acgagtaaag attttcacat cgtgaacgat accgccgccg ccatgtggta cacgtaatga
    25321 tgtatcgcgg acttcacgag ctttttcgcc aaagatcgca tgcaatagac gttcttctgc
    25381 agataattct gttacccctt ttggcgtgac tttccctaca agcaaatcgc catcttggac
    25441 ttctgcacca atacggatga tacccatttc gtctaaatct tttaatgcgt cttccccgac
    25501 attagggatt tcgcgtgtga tttcttcagg tcctaatttt gtatcgcggg cttctgattc
    25561 atattcttca atatggatag aagtgtatac gtcatctttt accaaacgac ggctcatgat
    25621 gatcgcatct tcatagttgt acccttccca agtcatgaaa ccaactaaaa cgttttggcc
    25681 tagagccatt tcgccttgtt ccatagatgg tccgtctgcc agagtatccc ctttttcgac
    25741 tttctcaccc aaatgaacga ttggacgttg gttgtagctt gttccggagt ttgaacgacg
    25801 gaattttgtt acatcgtatt tatctaatgc accgttgtcg cggcgtacgc ggatttcaga
    25861 agcatcaacg tattcaacga caccgtcatg tttacataac aaagcagctc ctgagtcatg
    25921 cgctgatttg tattccatac cagtaccaac ccatggtgct tgaggattga tcaacggcac
    25981 tgcttgacgc tgcatgttgg cacccatcaa ggcacggttg gagtcatcgt tttccaagaa
    26041 aggaatacat gctgtcgcaa cggcaactac ttgtttaggt gatacgtcca tgtagtctac
    26101 tttatcaata gatacttcta agttttcact ttgagcacga gccataacaa cgtcttgagc
    26161 aaatgttcca tcttcattca atgggctgtt tgcttgggct acgatgtaat ggtcttcgat
    26221 atcagctgtc aagtagtcga tttggtcagt aacacgacct gtttcacggt ctacacgacg
    26281 ataaggtgtt tcgataaatc cgaatttatt tactttcgca taactggaca aactattgat
    26341 caacccgatg tttgggcctt caggagtttc gatcggacac atacggccat agtgggagta
    26401 gtgaacgtca cgaacttcat aaccggcgcg gtcacgagtc aatccgccag gtcctaaggc
    26461 tgaaagacga cgtttgtgtg tcaattcgcc aagagggttt gtttgatcca tgaactgtga
    26521 caactgagaa gaaccaaaga attctttgat acttgccaca actggacgga tattgatcaa
    26581 ttgttgaggt gtcaatgttt cagtatcttg gatcgacatt ctttcacgaa ccacacgttc
    26641 catacgtgct agcccgatac ggaattggtt ttgtaaaagt tcaccgactg aacggatacg
    26701 acgatttcct aagtggtcga tatcatcgac attgccgata ccttccatca agttcaagaa
    26761 atagctcatt gaagcaatga tgtcggctgg acgaaccgtt tttacagctg cttctggata
    26821 gccgtttccg atcacgttta cttcacgttc agggtctctt ggtgaaaata ctttgatgac
    26881 ttggacagtc attggatctg tgaccacacc gtcttctgat ggatagtaag tcactgaatt
    26941 taggccatta tcaatgaaag gagctagtgt ttccatgact tggtgagtca gaactgttcc
    27001 tttttcaacg atgatctcgc ctgtttctgg atcaactaat gtttcagcta atgtcagatt
    27061 caatagacgt gttttcaaat ctaatttttt gtttactttg taacgaccaa cgtttgctaa
    27121 gtcgtaacgt tttggatcga agaaacgagc attcagcaag ttacgtgagc tgtctgctgt
    27181 ttttggttcg cctggacgca aacgttcgta aacgtctttc aaaccttctt ctgttcttga
    27241 gtcgcttgcg tttttatgca agtctttttc gactgtattg cgtaatgttt cgctgtcgcc
    27301 gaagatttca aagatcgtat catcggaacc aaatcctaac gcacgaacta atacggttaa
    27361 aggaatctta cgtgtacggt cgatacgtac ataagagatg tcttttgcat cagtttccat
    27421 ttctagccaa gctccacggt tagggataac agtagaaccg aatccttctt ttccgttttt
    27481 gtcgactttt ccatggaaat agacacctgg tgaacggact aactgagaaa cgattactcg
    27541 ctctgccccg ttgatgatga atgttccttg ttctgtcatt aatgggaaat caccgaagaa
    27601 tacttcttgt gctttgattt cgccagtctc gcggtttgtc aaacgcaatg ttacatgtaa
    27661 tggtgcagag tagtttgcat catgcgcacg agcttctgct actgtatact ttggttcttt
    27721 caattcataa tctacaaatt ccaaagatag attgccattg aagtcatcga ttggcaaaat
    27781 gtcttcaaac atttctctaa gtccctcatc taagaaccat tggtaagagt ctgtttgaat
    27841 ttcgatcaaa tttggtaatt ccaacacttc actgattctt gcgaaacttc tacgttcgcg
    27901 atgttttccg tattttacta cgtgtccagc caagctcttc acccctctaa aatgtcattc
    27961 aaaaagacta cccgatcaat tgttacaata atatttcaaa tattattttt aggtaacaac
    28021 agacattttt tgcggctttt aggcaaaaaa aaaccaaaaa tagaacattc tcttgaaacc
    28081 ttacttcgtg agcttctact tttgtttcct tatattaaag ccgaaacgga caatatttcg
    28141 tttctgcctt ttcttcatca gatagttttt gcaacttcat atactacact tattttctat
    28201 aaaagtcaac acttcctggc gtttcttctg ctttttcgca acattttctt aatgttttca
    28261 attttatgtt ttcaattatc tgacaattct taattgacag acctttttat ctcggttata
    28321 ctttagaaaa ttcattttgt ggaggaactt ctatgcgaac caacatcaac caaatcgcac
    28381 caacacaact gaataacttt tactttagct actttagata ggtttgtatt catcatgatg
    28441 gatacaaacg ttaaacgaca gtttgtatct atgatggcta aagaactttg tattgcagca
    28501 aaaagccacg tagacaaaat gctaagtggc tgagcagtga aaatacaaag taaaagtatc
    28561 aagcattcta ctttttcaat ttattttccg acttttcaat ccatttggac attttgtctg
    28621 aatggatttt ttgtttccca aaattaagga ggaaaaagaa atgaaaaaaa atataatcat
    28681 cggattaggc gctgccctgc tactagcttt aggaggttgc gcacaaaaag acaattccgc
    28741 agaggcatca gatggacaaa aaacaattgg cgtattgcaa cttgtacaac ataactcgtt
    28801 agatgctgcc tataaaggat tcaaagaagg attagcagaa gaagggtata aagaaggcga
    28861 taatctaaca atcgattatc aaaatgccca aaacaatcaa gacaacctga aaagtatgag
    28921 tgagaaactc gtcaaagaag atcctgattt gctacttgga atcgccactc cagcagcaca
    28981 atctttatta aatgaaacaa ccactatccc gatgactgtc acagcggtca cagacttgga
    29041 acaagcccat ttagtcacat ccaatgaaaa acctggcggc aatgtgactg gcacgacaga
    29101 tatggtgcca atcgaaaaac aaatcggctt actgttaaat atcgtgccag acgccaaaac
    29161 aatcggcatc atgtataaca acggtgaagc aaactctaag atccaagctg atttggcaga
    29221 aaaagaattg aaaaaagcgg gcgtcaaagt caaagtactg acagcaaatt ccacaaatga
    29281 tgtccaacaa gtgacgaaaa gtctagctaa agatgtagat ggaatctata ttcctactga
    29341 taacacgttc gcaagtgcgg caagtgttgt cggtgaagtc gcaaaagaaa caaaaacacc
    29401 tgtcgttgct ggcgcagttg atcaagtcaa agacggcggt ctagcaacag taggaatcga
    29461 ctacgaaagc ttgggtaaac aaacagggat catggcagca aaaatcttag acggcaagaa
    29521 tccaagtgaa cttcctgtcg aaacagcgaa tgatttggaa ctgtatgtca ataaagacat
    29581 ggccaaagca ttaggcatca atccagacag catcaaagaa gctgaataaa acacaggagg
    29641 attctcatga caaatttgct ctctcttttc ttatcatctg catcgcaagg ggtcttatgg
    29701 gccctacttg cgattggtgt atacgtcact ttccgtatct tggacattgc cgacttgact
    29761 gctgaaggaa gctttccttt aggtgcaggg atcgcggcag taagtattac aaatggctat
    29821 catccattga cagcttgtct tctaggcttt ttagggggtg cggcagcagg gcttgtttcc
    29881 ggactgcttc acacaaaatt gaaaattccc gcccttcttg cagggatcat caccatgact
    29941 ggtttatatt ctgtcacatc ccgtgtgatg ggtgcgccga acatctcttt attgggtcag
    30001 ccaacgatct ttcattgggt cgagtcattc ggcataacaa aagacaacgc tgtactgatc
    30061 gtcggtctgc tttttgcttt gatcgttgta tgtttacttg ttttgttctt acgtacagaa
    30121 acaggacttg ctattcgtgc aactggcgat aatctggcaa tgagcgaagc aaacgggatc
    30181 aacacagata atatgaagat catcggttat atgatttcta atggatgcat tgctctttct
    30241 ggctccttgc ttgcgcaaaa caacggcttt gctgatttaa attctggtat tggaacgatc
    30301 gtaattggac ttgcttctat tatcatcgca gaagttttgt tacgtaacca gccactattt
    30361 ctccgtctct tgactgtcgt tttaggtgcg gtcatttatc gttttatttt agcgttagtc
    30421 ttcgaactga atgttgaacc aagtgattcg aaacttgctt ctgctttcgt gctagtgatc
    30481 tgtctttctt tcccgcagat ccagcaaaaa ttccatttac ctcaaaaaaa gaaaggcggt
    30541 agtgtcaaat gaatgacgtc ttaacattgg aaaatctcca tcataccttt gaaaaaggaa
    30601 cgatcaacga aaatcatgtc ctgaaaggca ttaatctttc cttagatccc ggcgacttcg
    30661 tgacgatcat cggtggaaat ggggctggta aatctacttt gctgaacagc atcgccggta
    30721 cattttctgt cgatcagggt caaattttat tgaatggaaa aaatatcacg aaaaaatctg
    30781 ttgtcgaacg ttcgaaatcc atcagtcgcg tattccaaga tcctaaacta gggacagccg
    30841 ttcgtttaac ggtagaagaa aatcttgctc ttgctatgaa acgaggaaaa aaacgcggct
    30901 tcttccgtgg tgtcaaacca caagaccgaa catttttcaa agaccagctg gttcggctaa
    30961 atttggggtt ggaaaatcgt ttatcctcag aaatcggttt gctgtctgga ggacaacgtc
    31021 aagctattac gttattgatg gccacattac agcgtccaga actgatctta ttagatgaac
    31081 acactgcagc gcttgaccct gctacttcca aaacggtgat ggaattgaca gaaaagctga
    31141 tcactgagca gaatctcact gcttttatgg tcactcatga catggaagac gcgatccgct
    31201 atgggagtcg actgatcatg ctgcatcaag gaaggatcgt cgtagatatc cagcaagagg
    31261 aaaaacagca gctcacagtg ccccaattat tagcgttgtt caaacaaaac agcggcacgg
    31321 cattaaaaga cgatcaagta cttttatctt cataatcaga aagaggatgg gacaaaaaat
    31381 gaacgcccac cctcttcttt tttctctttc cttctttcgt ttttgcttta tgtatcaacc
    31441 gttgtttctt cgaaaactat tttgttctct ccttcttcct gatagaatgg atgggaaagg
    31501 agcttgatgc catgacaacg aaagacaagg tacttcaatt attgaaaaag acaccagatt
    31561 ttctttccgg agaaaagttg gcacagcaac tggaagtttc ccggacaagt atttggaaag
    31621 ccatcaaaga attggaaaag gcaggctatc gttttgagca cgggcctaca gggtatcgtt
    31681 acctgccctc tgacgtacta gatgcaaaag aaatccagca agagctacgg tcgtttattc
    31741 ctgatttgaa agtacaaacg atgttgactt ctgaatctac gatgaaagat gccaaactgg
    31801 cagcagctca aggagattct gtttcgaagt tatttgttgc tgatacacaa gaacaagctc
    31861 gtggacgttt tggacggcct tttttcgctg aacctggacg aggaatctac atgagtctct
    31921 tgctccatcc agacaaacgg tttgatgaat tgcctcaata tacgatcatc gcagccagtg
    31981 cctgtgtgaa agccattgaa tctgtaaccg gtcgttctgc ttcgatcaaa tgggtcaatg
    32041 atatctacat ggatggaaaa aaagtctgtg ggattttatc tgaagcagtc agtgatatgg
    32101 aaagcggcag gatcagtcat atcatcattg ggatcgggct taatttttct gttcctcaaa
    32161 atcattttcc agctgatctc caagaaaaag ccatctctgt ctttcccgac ggacaagcta
    32221 gtgtcacaag gaatcagcta atcagcaaaa tctggcaaga attgttcact tatctttctc
    32281 acccagaaaa agcgttggac ttctataaag aaaaatcatt cgtattggga aaaaccgtgt
    32341 cgttcaatca aaaaggaacg acttatgtcg gtcttgcaaa agaaatcggt gatcatggcg
    32401 aactgatcgt ccaaacaaat gaacagaaga tgagtttgtc ttctggcgaa atcagtctca
    32461 catctatccg tacgcaataa ttatcagatt tcacaaaagg acacctgatc tcatacagtg
    32521 tccttttttc tgaattctct tcaaaagact ctccttattc tatcagctga cagtttctct
    32581 atcctctaga aaaaggtatt ctaaaaatat ctataaagca tggtacactt tctttaaatg
    32641 aatcaatgca aaaaacaaga gagtcatcag tttacacgat caaaagagat agcaggccgc
    32701 ttcctttttc aatcttatag atagaccatg ggtaacgata aaaacaaaaa agaacttgcg
    32761 acttattttg ccgtaagttc ttttttagct agtgccttaa tctcttaatc ccaacaaacg
    32821 tctcgcatag atcacctgac cagagtgcat agcagcatca tcaataatag aaacgagacg
    32881 cattcctcgt gtgactggtg gtgtccagtt ctcgtcaatt acttcatcta gctcggaagg
    32941 ttccagcctt cttaagtaag aaatagcaaa atcagcagct cctcgcaagt atcctagcag
    33001 atcttctatt tgattagctt ggatctgctt tgcttgttcc agactatgct tccaatcttg
    33061 ttcgttatct gctatcgtca tcggaaaccg gctttcccag tcttgactat gccaaattgg
    33121 agaagttcct gctaaatcag caatctggaa atctaattct cgagcggtat gccaagccag
    33181 ccaagtcatt gatttgatct gcggggctct atcttccact ggaaaacgat tggcctcctc
    33241 aatagagacg ccttcaaaca aacgttcaaa ccgatcgtat gcccgatcca cactctcgca
    33301 tagcatatct aaataatcca tttattttcc tcctttttat ttatcctact tactttttcc
    33361 taattctagg atacctactt atatgttact acacaccctt attttttaca ctttaacagt
    33421 caccatcatc aatagtttgg agattgaaag aaaaagatag tgtcgaacta tcaattgaac
    33481 aagaagcaac aatatgaacg tttcattaat attctttaaa taaacaatga tttttcaaac
    33541 gaacagttta agactaggag aaaaagagta taataggaaa atgtttgaaa ggaaaggaag
    33601 gggaaaaatg aaaaaaaggg caagtttact agtcctagtt gctttgttgt tcaatatctt
    33661 ttcgtatggt gtggccgttt cagcggaaac atacattcct aaagaccctg ggaatattta
    33721 taatgatcta actggtaact atggcttgtt aggaattgct agtcaattcc acgtatttgc
    33781 taaaaataag acaactgtga atgcacatac agacggaaat atagcaacac atgaacttga
    33841 tgcaaaaaat aattttggta cagagcttca tgaaggttta ttgaaccaag aaataaatta
    33901 tatacaaaaa actgattctt tcgctaattc ttcaggcata cctacaggta gcacaagaat
    33961 gaataaattt gtcgttggca gtaacgtgga tgtagcactt caagatggtc atcctacagt
    34021 caatggatcc aggcttgatc acttaactcc agatgatttt tataaagatc gtgctgggca
    34081 tgaatatatt gatttcgagc aggaatttgc taaattgaat gtagcagcaa atgatcttgc
    34141 aacgatcact ccagctaaaa catatactgc tgcagatttt ccagacatga acaatcggac
    34201 aatcgattta actgatttat cagataccgg gtttttattg gtaaatatcg atgcagaagt
    34261 gttaacgatg aatacgccac ttcaaatcat taatccaaat gatcaagtag ttgtatttaa
    34321 tgtcatcaac agttcttctg cattgaacgt acagtcacca atcaaataca atgatcgttc
    34381 aaatcatgaa acagaagatt tttcggatgc gaatatctct tggaattttg gaaatgagat
    34441 gactgatttg acaatcagtg ctccttttca aggaacaatc cttgcgccta atgctactat
    34501 ccgcattact caaaaccaag acggaacgat tataggaaca aatgtgattt tagatgcagc
    34561 taccaatcgt tgggatccta acgaaatatt tatcagcgac aatggtacag atacgacaga
    34621 cacaagcgac agtacagata catctaccac aagtgatagc tcagattcat caacttcaag
    34681 cgacagtaca gatactacct ctaccacaag tgatagcacg gatacatcag cttcaagcga
    34741 cagtacagat actacctcta ccacaagtga tagcac
//
LOCUS       DAHHPA010000018        34687 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.019, whole genome shotgun sequence.
ACCESSION   DAHHPA010000018 DAHHPA010000000
VERSION     DAHHPA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.019"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            591..1280
                     /locus_tag="NBH55_000809"
     CDS             591..1280
                     /locus_tag="NBH55_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1508814.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="NBH55_000810"
     CDS             1267..2475
                     /locus_tag="NBH55_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD1508815.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="NBH55_000811"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="NBH55_000811"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCD1508816.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="NBH55_000812"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="NBH55_000812"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCD1508817.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="NBH55_000813"
     CDS             complement(6578..7273)
                     /locus_tag="NBH55_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCD1508818.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="NBH55_000814"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="NBH55_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCD1508819.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="NBH55_000815"
     CDS             complement(8472..8663)
                     /locus_tag="NBH55_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCD1508820.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="NBH55_000816"
     CDS             complement(8684..9574)
                     /locus_tag="NBH55_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCD1508821.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="NBH55_000817"
     CDS             complement(9561..10328)
                     /locus_tag="NBH55_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCD1508822.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="NBH55_000818"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="NBH55_000818"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCD1508823.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="NBH55_000819"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="NBH55_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCD1508824.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="NBH55_000820"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="NBH55_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCD1508825.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="NBH55_000821"
     CDS             14899..15282
                     /locus_tag="NBH55_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508826.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="NBH55_000822"
     CDS             complement(15450..16208)
                     /locus_tag="NBH55_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCD1508827.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="NBH55_000823"
     CDS             complement(16226..17044)
                     /locus_tag="NBH55_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCD1508828.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="NBH55_000824"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="NBH55_000824"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCD1508829.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="NBH55_000825"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="NBH55_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCD1508830.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="NBH55_000826"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="NBH55_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCD1508831.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="NBH55_000827"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="NBH55_000827"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCD1508832.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="NBH55_000828"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="NBH55_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCD1508833.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="NBH55_000829"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="NBH55_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCD1508834.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="NBH55_000830"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="NBH55_000830"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCD1508835.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="NBH55_000831"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="NBH55_000831"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCD1508836.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="NBH55_000832"
     CDS             27086..27385
                     /locus_tag="NBH55_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCD1508837.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="NBH55_000833"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="NBH55_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1508838.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="NBH55_000834"
     CDS             27973..28209
                     /locus_tag="NBH55_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCD1508839.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="NBH55_000835"
     CDS             28352..30325
                     /locus_tag="NBH55_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCD1508840.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="NBH55_000836"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="NBH55_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCD1508841.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="NBH55_000837"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="NBH55_000837"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCD1508842.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="NBH55_000838"
     CDS             33204..34496
                     /locus_tag="NBH55_000838"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCD1508843.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10569 a         6743 c         6858 g        10517 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaa
//
LOCUS       DAHHPA010000019        34665 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.020, whole genome shotgun sequence.
ACCESSION   DAHHPA010000019 DAHHPA010000000
VERSION     DAHHPA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.020"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(316..2208)
                     /locus_tag="NBH55_000839"
     CDS             complement(316..2208)
                     /locus_tag="NBH55_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1508844.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2205..3932)
                     /locus_tag="NBH55_000840"
     CDS             complement(2205..3932)
                     /locus_tag="NBH55_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1508845.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3948..4598)
                     /locus_tag="NBH55_000841"
     CDS             complement(3948..4598)
                     /locus_tag="NBH55_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCD1508846.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4802..5098
                     /locus_tag="NBH55_000842"
     CDS             4802..5098
                     /locus_tag="NBH55_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD1508847.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5290..5910
                     /locus_tag="NBH55_000843"
     CDS             5290..5910
                     /locus_tag="NBH55_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCD1508848.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6002..7168)
                     /locus_tag="NBH55_000844"
     CDS             complement(6002..7168)
                     /locus_tag="NBH55_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1508849.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7180..7533)
                     /locus_tag="NBH55_000845"
     CDS             complement(7180..7533)
                     /locus_tag="NBH55_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCD1508850.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7661..8683
                     /locus_tag="NBH55_000846"
     CDS             7661..8683
                     /locus_tag="NBH55_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCD1508851.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8702..8863
                     /locus_tag="NBH55_000847"
     CDS             8702..8863
                     /locus_tag="NBH55_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508852.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9122..9556)
                     /locus_tag="NBH55_000848"
     CDS             complement(9122..9556)
                     /locus_tag="NBH55_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508853.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9666..11525)
                     /locus_tag="NBH55_000849"
     CDS             complement(9666..11525)
                     /locus_tag="NBH55_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCD1508854.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDHSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11643..12803)
                     /locus_tag="NBH55_000850"
     CDS             complement(11643..12803)
                     /locus_tag="NBH55_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCD1508855.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13110..14534)
                     /gene="araA"
                     /locus_tag="NBH55_000851"
     CDS             complement(13110..14534)
                     /gene="araA"
                     /locus_tag="NBH55_000851"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCD1508856.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14550..15248)
                     /locus_tag="NBH55_000852"
     CDS             complement(14550..15248)
                     /locus_tag="NBH55_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCD1508857.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15205..16866)
                     /locus_tag="NBH55_000853"
     CDS             complement(15205..16866)
                     /locus_tag="NBH55_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCD1508858.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17084..18484
                     /locus_tag="NBH55_000854"
     CDS             17084..18484
                     /locus_tag="NBH55_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCD1508859.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18736..19815)
                     /locus_tag="NBH55_000855"
     CDS             complement(18736..19815)
                     /locus_tag="NBH55_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1508860.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19901..20860)
                     /locus_tag="NBH55_000856"
     CDS             complement(19901..20860)
                     /locus_tag="NBH55_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCD1508861.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20872..22383)
                     /locus_tag="NBH55_000857"
     CDS             complement(20872..22383)
                     /locus_tag="NBH55_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCD1508862.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22405..22968)
                     /locus_tag="NBH55_000858"
     CDS             complement(22405..22968)
                     /locus_tag="NBH55_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCD1508863.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22974..23837)
                     /locus_tag="NBH55_000859"
     CDS             complement(22974..23837)
                     /locus_tag="NBH55_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD1508864.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23851..24747)
                     /locus_tag="NBH55_000860"
     CDS             complement(23851..24747)
                     /locus_tag="NBH55_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1508865.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24922..26211)
                     /locus_tag="NBH55_000861"
     CDS             complement(24922..26211)
                     /locus_tag="NBH55_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1508866.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26183..27700)
                     /locus_tag="NBH55_000862"
     CDS             complement(26183..27700)
                     /locus_tag="NBH55_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCD1508867.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27898..28788
                     /locus_tag="NBH55_000863"
     CDS             27898..28788
                     /locus_tag="NBH55_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCD1508868.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28861..29685)
                     /gene="nadE"
                     /locus_tag="NBH55_000864"
     CDS             complement(28861..29685)
                     /gene="nadE"
                     /locus_tag="NBH55_000864"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCD1508869.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29697..31169)
                     /locus_tag="NBH55_000865"
     CDS             complement(29697..31169)
                     /locus_tag="NBH55_000865"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCD1508870.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31806..32168)
                     /locus_tag="NBH55_000866"
     CDS             complement(31806..32168)
                     /locus_tag="NBH55_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCD1508871.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32391..33143
                     /locus_tag="NBH55_000867"
     CDS             32391..33143
                     /locus_tag="NBH55_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1508872.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33283..34206)
                     /locus_tag="NBH55_000868"
     CDS             complement(33283..34206)
                     /locus_tag="NBH55_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1508873.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34221..34493)
                     /locus_tag="NBH55_000869"
     CDS             complement(34221..34493)
                     /locus_tag="NBH55_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508874.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10231 a         7214 c         5979 g        11241 t
ORIGIN      
        1 taccaagagg ttgtgagcct gccgtttaac tccgagtcac aaggagcaat ttcaaaaaac
       61 agcttctcag gtttgttgac tattgtgact tgtgaccgag gagttggcag gagaacaccg
      121 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagata caaaacggaa
      181 aaatagcttc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
      241 ccgtttattc ggaaaaaggg actgtgacac gacttttgtc acagccccta aaaaaaataa
      301 taaatttgta ctattctatg aagcttcttc ttcagaaaac tgactgttat aaagatctgc
      361 atagaatccg tctttttcca tcaatgtatc gtggtttcct gtttctacga tcgaaccatg
      421 attcatcaca ataatgttgt ctgcatcgcg tattgtagac aagcggtgag caacaacgaa
      481 acttgtccgg ttttccaata ggcggttcat cgctttttga atcagtattt ctgttcgtgt
      541 atctacacta gatgttgctt catcaaggat caggacatct ggatttgcta agaatgcccg
      601 cgcaattgtg attagctgac gctgcccttg cgaaatattg cttgcatcct cgtttaatac
      661 agtgttatat ccttctggca atttacgaac aaaatcgtct acgtgagcag ctttagcagc
      721 ctcaatgatt tgttcgtctg ttgcatcgtc gttcccatat ttgatattgt catagatact
      781 tcctgtaaac agccaagtat cttgcagtac cattgaaaac tgtgcgcgca attcatcacg
      841 tgaaagatca cgggtatcta caccatcgta acgaatacta cctccagaaa catcgtagaa
      901 ccgttcaaga aggttgatca gtgtcgtctt ccctgcacca gtcggaccta caatagcgac
      961 catctcacca ggtttgacat ccaagttaaa atcagtcatt agcaagttat ctggtgtgta
     1021 gccgaattga acattttcaa aacgaacttt gtatggtgta tctttttctt ctggaaggcc
     1081 agatttttca ttggacatct cttcttcatc caacacttca aatacccgtt cggctgaagc
     1141 aactgttgat tggatagtgt tcatcaaact tgcaagttga gtgattggtt gagagaactg
     1201 tgttgtatat tgcaagaacg cttgaacatc ccctaatgtc atgttcccat ttgcaacttt
     1261 tactccgcct agtacagcga cgaacacata gcctagattt ttaacgaaat tcatcaacgg
     1321 catgatgatt gccgaaataa attgagcttt ccaaccagcg gcatacagat tctgattttc
     1381 tttttcaaat acttcctggt ctttttcagt atgattgaat gttttaacaa tggtatgacc
     1441 accatatgtt tcttctactt ggttattcaa taaacctaaa cttttttgct gatctgcgaa
     1501 aaacacttgt gatttcggcg ctacgatcat cacgatgatc aagcttaaag ggatcatcaa
     1561 taacgcgact aacgtcaatt gccagctgat tgaaagcatc atcacgatga ccccaatcaa
     1621 tgtgactgca ctagttacaa attgagtcaa attttgctgc agcgtgcttg cgatgttatc
     1681 catatcattg attgctcgag acatgatatc cccatttgta tgtgtatcat aataggaaat
     1741 cggtacacga ttcattttgg cttccaaatc atgacgtaat tcataaactg ttcgttggga
     1801 aacacgtgtc ataataaatt gctgtaaaaa gttgaataca gcagaaatca aatacatcac
     1861 gattactgtc gcaatgatct gcgcgatttt atcaaagtct atcggaaatt ttgttacttg
     1921 ttggccttgt tgcatctgtt gagcgccagc cattacacct ttgaatattt ctgtggttgc
     1981 tttccccaag acctttggtg tttggatttg gaagatcacg gctgctattg ctagtacaaa
     2041 tacagcgatg atcgcaacca tacgtttaga catatagcca aacaatcggc gtaccgttcc
     2101 ccagaaattt ttaggctttt caccttttac accgagattt ctgccaggtc caccatgtcc
     2161 tggaccgcct gctggtccat gagagggacg tgtgtttgtt ttactcatgc taagtcctcc
     2221 tctcttagtt gtgaatgaac gatttcttga taagtttcgt ttgttgctaa tagttcttca
     2281 tgtgttcctt tgccaaccag ttttccttca tctagcacaa ggatcatatc tgcatccatt
     2341 actgtactta ctcgttgagc aactaaaacg gtgatacttt ctttcatatt cgttttcaat
     2401 gcttggcgaa gattggcatc cgttttgaaa tccaacgctg agaaggaatc atcaaacaca
     2461 tagatatctg ctgttttgac taatgcccgt gcaattgcca gacgctgtct ttgtccacca
     2521 gagaagtttc ctcctccttg ttccacatgg ctatctaagc catcttccat ctctgaaaca
     2581 aaatctttag cttgtgcgat ttctaatgct ttccaaatca tttcatctgt tgcatccggc
     2641 ttaccatact gcatattgtc tcggatcgtt ccagagaaca gaactgcttt ttgaggaaca
     2701 aagcccatta attctcgcaa attatattga gtcatgtcac taatgtccgt tccatttatt
     2761 tggatcgaac cagattcgat gtcatataat cgagggatca aattgactaa ggtcgtttta
     2821 cctgaaccag tcccccctat gattgcgacg atctcgcccg acttggcttg gaaatcaata
     2881 tcttccaatg caagtttttc tgctccatga tatcgataat tgacatgatt gaaggccaat
     2941 gttgcgtttt ttccagcaaa agaaactgtt ttaggatttt cagggtcatg gataccgatt
     3001 ttttcatcca acacttcatt aatacggtca gctgaagctt gtgcacgagg caccatgata
     3061 aaaatagcgg aaagcatcat aaagctgatc aagatctgca tcgcataagt cataaatgca
     3121 atcagattac cgacttccaa agtcatatct gctatgtagt gtccaccaaa ccaagtgatt
     3181 gctacgtttg taccgctcat gatcaatgtc atcattggca tcatcaatgc gacgatcgta
     3241 ttaactttga ttgccgtatt tgtataatct ttattcgcta aatcaaaacg attttcttca
     3301 aagcgtgtct tatcgaatgc tcgaatcact ctgacccctg tcaatccttc tcgaaatacc
     3361 aggtttaaac ggtcagtttt cttttgcata cttttgaata atggaacagc aaagtacatg
     3421 attccgccga tcaaaataat cattactggg atgacaacta agaagatttt tgtcaactga
     3481 tgatcttttt gataagcaag tatgctcgcg ccaatcaacg tgattggtgc attgatcatc
     3541 atacgtaaaa acatttgtgt aaccatttgg atttgagtca catcattggt cgttcttgtg
     3601 atcaaagagg ccgttccata tttatcaaac gcatcttttg tcagactctc tgatttttta
     3661 tagacatccg tacgcaattg cttacccagt ttctgtgatt cacgagtagc aaaaaatgta
     3721 tttgcgatag cagctaagat actgatcaaa gaaaagccga tcatcacaaa acctgtatgc
     3781 caaatataat cgacatctcc ttgcgctacc cctttatcaa taatattgga ggtaagtgtc
     3841 ggcaaatata aatctgccag tacttggatc accatgaaaa ctgcagctgc aattgctgaa
     3901 gtcaaggaga tccgttttac gatttttagc atctagttcc cttctttcta tttatatact
     3961 ccgtttttca gccaagaaag tttcaaattg aattcactga ttgcttgctc tactgagtaa
     4021 tcttcagatg ttgaaaataa gcgatacgaa tgcgcaattg ttgtaaatac agtattcatc
     4081 actaattgga tcagtaattc caattcatga tcatttttta ctttcagctt cgtccgatcg
     4141 atcaattcaa gcaactcttt tgcatcttgt ttgtgttctt tatgtggatc gtgaggcgtt
     4201 ttttggcgac tcgtatgagg agcgactttt cttgaggcac ggtaatccat gttcataaac
     4261 aagttacgat aaaaagaagc attctcgcct tccaaaatct cataaatcat tttagaaaaa
     4321 taggattcga atcctttgaa tagatcacca tcggattctt tcattagttg taacatatct
     4381 ttagagctgt cacgtctcat tgtctcaaag taataaaaat aaagatcctc tttatcttca
     4441 aaatactgat agaaactgcc tctagggata tccgctaatt tgacgatatt agcaatcgaa
     4501 gcttctttta agggtactct agaaaactcg attctcgcag cctcgattag tcttttttgt
     4561 ttttctttag gcaaatggaa aaatgtctgt gtcggcatat ggattctcct ttctttcaag
     4621 atgacacatt gtcatatgac gctatgtcat tataacacct cctacctgtt ttgcaagaca
     4681 tttgcatagt ttttttcatt atcatccgat acattaaaaa agtgataaga ggaatccttc
     4741 catttgaatt aatgagagtt gcccattaaa atagccacat actaaacaaa ggatgagata
     4801 aatggcccaa tcaattagtg tccaagactt caataaattg cctctcgatg ctgacagcat
     4861 cgttttagat acaagaaata ccttagatta cacaaaaaac cacttaagcc actcgctttc
     4921 tatccccgct gctatcttgc caagacaatt aaaaaatcta gatccaaaga aactttatta
     4981 tattctaagc tatacaggca ggcgatcaga agcattagcc gatcaactta ctgctaatgg
     5041 ttttcgtgct gtttatatca ttggtgggat ggaagctctt cagtcgagtg ttgcttgaat
     5101 attgcttgtt agattcaaaa gaggctgtga caaaaaatcg tgtcacagcc tcttttatac
     5161 cctgtctttt tttagttatt tattcttcaa ccttatcaat atgacttgat agccttttct
     5221 ttttcgagta aactgatttt gttagtcata gttataacaa tcatagcaat aaaaggagaa
     5281 ggtaagcata tggcatctgt tttagtagtc aaaggtcatc ccctaactgc agaagaatcg
     5341 cgcacagtta aggcattgac gtctttttta acaagttata aagaaaatca tccagatgat
     5401 gaagtgactg ttttggaact ttatcgcgat gatattccgg aaatcgatga agaattatta
     5461 tcaggttggg aagcattacg tgctggtgca gaattcactt ccttatcaga aagtcaacaa
     5521 caaaaaattg ctcgtttcaa tgaattgact gatcagttcc ttgctgctga taaaatcgtt
     5581 atcgcaaacg ccttatggaa tctaaatatc ccaacaaaat taaaagcttg gttcgatacc
     5641 gtcaatgtag ctggaaaaac gttccgctat acagaaaacg ggccagaagg attagtgaca
     5701 ggtaaaaaag cactgcacat ccaatctaat ggcggtgtat acaacggaca agactttgcc
     5761 tctcaatatg taaaaggtat tttaaacttt gtaggcatcg atcaagtaga tcagctattc
     5821 atcgaaggaa tcgattatga ccctgaccgt gcagatgagt tgatgcaaaa tgcattagat
     5881 aaagctgctg ctttaggtaa atcattttaa aaattgagat aaatgcatac aaaagagaca
     5941 gaggaaaaaa tcttcaaagc agcatttgtg ccttggatat tttcccatct gtctcttttt
     6001 gttattcttt ctgctttttc aacggatttt cttcataaag agaagagatt gttgtgtacc
     6061 attcaaaaac atgagtaatc actactttag ctgctgcata aacaggaatc cccaagatca
     6121 caccgatgat tccgaacaat ttcccagaag tcagcagaac taataaaatc gttactggat
     6181 ggattgctaa ctgattccct agtactaatg gagagatcaa gcgtccttct attgtctgct
     6241 ctactgcaaa aacgatcagt actttgatga tcatgaatgg acctgctaca atggctaaaa
     6301 agatagccgg aatcatcgct aaaaatgatc ctaagtaagg gatcaggttt aaaaaacccg
     6361 caataatccc caacgtgaca gcatactcta aaccaatgac cgcaaaacca atcataaaca
     6421 tgattgccac agcaaaagcc acagttagtt gtcctcttat ataagaagaa acttgactat
     6481 tcatttcact cagcacattc aacgtcggtt tttgcatgcg tgtcggtaaa aatttgacaa
     6541 aataaggtgc taattgttta ccgtctttca atagataaaa taagataaac ggcatcgtca
     6601 gcagagccac taaaatagtc gctaccgcgc cgacaaaact tccaagactc tgaactgtac
     6661 tcttagatat atcttggatc atatcgcctg ccgaattcat gaacttatca cctgctgttt
     6721 ccaactgttc gcgaaattgg ttgaacagcg ggtcccgcaa aatctcggtt aatttattat
     6781 caatcgtatc tacatattga gggaagttac tgatgaagct cactgtttgt tcctgaatct
     6841 tagggatgat cactacgctc ccccatgcga ttagtgcaac aaccaaaaca aacagcccaa
     6901 tgatactgta aagccttggt acttttcgct tttcaaagta atcaactacc ggattcatca
     6961 agtaatacaa gattcctgct aaaatgatag gcagacctac gattgctaaa aattgccaga
     7021 ttggtgcaaa caaatatgaa acttttgtaa ataaaaagac gatcagcaat attaacaata
     7081 caacaagtaa ggctgtgact acttgattat ttaaaaacca gcgccaaaac cacgataccg
     7141 gtctgttctt cttatttttt ccttccataa ttcctcctgt tagttataaa aaatttggct
     7201 tcccaaacca atagcaggca gtctatgcgg atgaagatag attccatttg caatttgtcc
     7261 ttcttctgga caagcatcaa aaatcaatga tacgtgagct actgtcttta aattcccatt
     7321 ggccatcgga ccgacataat cgatcgtata tacctgatca tcaaaagcaa ttgttccgcc
     7381 tgctttcaaa gaaaaaggtt cgtcttttga tacttcttga atcaccgaat attttcgtaa
     7441 ggcagctgtt gctgttttat cgaacaaaat cagcatgggt tcttccttgt ttattgcttc
     7501 tgtcccaatt tctgtaatct ttgcctgaat cattgcactc tctcctttaa ttgctttcaa
     7561 aaacagtata acacattctc attctttagc cgtgtggaat aattatgtga tagaataaaa
     7621 acgaaaacta tttcattaca attaggagga atcagctcat atgatcgaaa agataatttt
     7681 aggaacctat acgcgtcgcg aaagtaaagg aatctatacg atcgaccttg atacagaaaa
     7741 agaagaatta gccaaccttt cattagctgt tgaagaaaat agccctacct atgtagcaaa
     7801 aagtaaagct ggcaatcttt acacagttac tagtgtggat ggattaggtg gtgccggtgc
     7861 gtacgataag gattttcatt ttttgaatgc ggtaacagag tctggcgctc ctttgtgtta
     7921 tgtagcggtt gatgaagcac gtcaattact atacggggcg aactatcata aaggtgaagt
     7981 aaatgtctac catatattag gagatggcga actgaaagca gcagattcaa tattccatca
     8041 agaagcgaca ggtcctcata aaaaccaaga tcatgcgcat gtccactata cggatctgac
     8101 tcctgaccaa cgattagtcg tatgtgatct tggtacagat cgtgtgtata cttatgatgt
     8161 gtccgaaaac ggcaaattga atcttgtaac taccttcact gcagagccag gaacaggccc
     8221 tcgccatctt gtctttcatc caaatggaca attcgcttat ttatttggtg aattagacag
     8281 cacagtccgc gtgctttctt ataataaaga aacaggtgct tttgaagaat tacagaaaat
     8341 atcgaccttg ccagaagaat tcgacggtga aaatggaggg gcagctatcc gcatttcaaa
     8401 tgatggcaag ttcctttatg cttctaaccg aggacataac tcgattgctg tattcgcagt
     8461 ttttgaagat ggttcccaca ttcaaaacaa gcaaatcatc tcaacagaag gggattttcc
     8521 ccgcgatttc gcactagatc caagcaataa ttttgtcgtg tgtgccaatc agaacaccga
     8581 taatctgaca ttatatcgcc gtcatcctga aacaggatta ctggaattac tgaaaaaaga
     8641 tgtctatgta ccagaatgtg tctgtgttta ttttgatcgt tagtaagaaa aggagaatga
     8701 aatgaatcag caagacgtat tagacagact acgggaagaa ctaaatattc ctttctttga
     8761 tgggaaaatc gaagataaag agtatagcga agaggaatat cagaaactca aagccgattt
     8821 acagaactac tttgaacaat atgtacagaa tgtagaaaac taatcagctt tccatactat
     8881 ctgaaaagaa catcaggaca agacttttcc gtctatcctg atgttctttt ctacaaataa
     8941 aaaaccgccg agatggttag actcgacgga aaaggagtat ttactcgatt ttgagtaagt
     9001 acaaggttgt tgttggttat aggtatgtca ttatcataaa ggattttgat cactctatca
     9061 taaaggtaat attacattta tattagataa ctctagtttt tattgttatc aaagatttct
     9121 tttatttttt caacaacaaa ttagtcattt tttggatggc agctgtcaat tggtctgtcg
     9181 cttggtcttg ttttgcttca gatgtattga atagatcttc atctacccct gattcaagat
     9241 cgatactttg gatcatttct tcacagcctg ctacatatga aagataagct ttttcaaaat
     9301 ttttatgaat gcccattgct tttgctggtg gacgcaaagt actgactttc ataagcattg
     9361 cccgatattt ttttgttcct tcttcaaata acaggatgat ctcttcaaga gtcgatttgt
     9421 ccagttcact tgtttttcct tcatcaattg cgtttctgat cacttcatat ttcggatgca
     9481 ttccttctcc gattgcttct gtttccttga cgatttgatc gatggactgc acataatagc
     9541 caatattcgg taacatgttc gttttcctac tttctccatt gttgttatta tttattctac
     9601 ttgtttttat cctcaaatga caagtacttg aaaaaattta atggtttcta tacagctcgc
     9661 cattcttatt cgtgctcaaa gaaaccatct gctaaactat attgtttccc tgcgacaatg
     9721 tcatattgga tcaattgata ttcgtcgtaa atctcttgtt gcgagcgatt gaattgcgct
     9781 tctttatacc actgtccgtt ttgatagtac agttctcgtt caaacgccgg aagttcctgt
     9841 tctaatgata aaagcagctg ataaaagcca tttgttgtct gattcgtctg ttccattaga
     9901 ttcgctgcaa aataaaacgg actggtgatt gcatcgtgag tcgtttgctt ttctaattct
     9961 cctttactgt cgaacataag aaattgtgtt tcatgcaatg tttgtttgtc attttttgct
    10021 tggatcgtat ctgaatagat ccctggaaga tgatcccccc aaaaaacaac taaagtcctt
    10081 ctagaaagat tcttcaattc ttccgtgaac tgcttcagtg ccgtgctgct ataggaaata
    10141 tcttgcagat aattctctaa gtcaagtgtc ccacttccat ctgaagtttt tgcaggatag
    10201 tctagattat catactttcc atcgtagggc atatgtgtct gcatcgtgac taaatgaatg
    10261 aactgaggtg ttttttcttc cttgagaagt gtcattactt ctttgtaagc tgactcatca
    10321 gaaatatatg gattattctc tatcgtatcc gtataggtca tcgtacgctc gctgatgaac
    10381 tggtcaaatc ctaatgtctg ataaacatcc tctcttttat acatcgaagt attgtatgga
    10441 tggatcgctg tcgtctggta tgattgtgcg tttaacgctg aaacgataga aggaagctga
    10501 tccatttttg gtacaagcat cgtgtaaggg gtggtgagtt gcgcattaaa taacgccata
    10561 gaaaaaccag tcaatgcttc aaattcgata tttgctgttc caccgccata gttttgagaa
    10621 agcatattgc cactatatgt ttgatcagct acttcataat aatctgccaa tggctcacca
    10681 gatacttcta cgccgtttaa tcgactatga tctgagaaac tttcactcat cacgaacacg
    10741 atgttcggcg actcttcctc tacagtttta tttttttcat cggctagctt ctggtatttt
    10801 tcagtgattt ctttgatctt ttctttcgaa tatccttcag gttcatccat tggttctacc
    10861 ttcagattgt acaaaaaccc accgataaaa ccagtgttat agtagttcat tttctggctg
    10921 taaggtatcc atagtgccgt tttattatat gcttttcgca acaaattatc gggattgttg
    10981 aaattactga tataccctag cagtccaatt gccacaagca gtgtcgttaa tcggatcaat
    11041 tggaatcgcc ttcctttttt caggcttcga aacatgtacc agcaaaacaa tcctatcacg
    11101 gttcctgcgg cagctaagat cactgtaaac agcatagtgc cggtcatatc tttcagcaat
    11161 ccgatttcag tgatcatctt caaatcgtct ggataaatcg gttcttgccg ataactcatt
    11221 ttcatataat ttgcaaaacc taatacaccg atggctacga catacgtaag ggaacctaaa
    11281 gggacagatc ctgctagtgc aatcagaaaa ataagaataa tcagcaagac gagacaggct
    11341 aagaagaatt tttccgtatg ccaagaaaaa gcaaatttca acgcaaggtc tacggataga
    11401 tcattttgac accactgcaa aaataaatta ctaaaaacaa caagaaaaaa gatactgatg
    11461 attgctgctg ttccttttaa aatctttttc tgtttggtcg aaagtctgct tatatatctt
    11521 ttcaaatgct tgctccttca tttctatcaa taaaagaatg ttgcttctct tattttagtt
    11581 gtacgaaatt cgaaagtaaa gaacggaaag aagaattaat gaaatccaaa tattctcttt
    11641 ctttatggcc tttctggttt ctttaataag cggacctttc gtactccaga aattttttct
    11701 agctgttgtt tcacttgatt gagctgttca aaatcagtac tgtctatgtc aatcaatatg
    11761 tagacatgct cattcttatg gttacgcgat atatctgcga tcgctagctc gttctcattc
    11821 agcagagaaa atatctctgc taatatattg gttttctttt gataaaatat agtgaatcga
    11881 aacggcgctt ggaaaaacat tcgtgcggat ggataattga ccgcttctct aaccgtaccg
    11941 aacaaaagat aatcttttaa ggcttcaact gccaaacgtc cgctgtctgc caaagcttcc
    12001 tgagtcgatc cccctagatg aggcaacata agtatacggt cattttctaa gaactcttct
    12061 tctggaaaat cagtcaagta tttagccaga tgttgttttt ccaatgcttg cataacagca
    12121 tatttatcaa caatttcact tcttcctaca ttgataagta cagcatcttt tttcattttt
    12181 tctatattcg cctgatcgat caatcctttt gtatcttctg tcaacggcag catgactacg
    12241 atgtaatccg aggtagaaag aactgtttct aaatctgctt gccgaacata atccaactgg
    12301 gcatcgcgga tagaataacc caagacatcc atccctaagc tatagcaact tagcgctact
    12361 tttgtgccta tcgctccaag accaagaaga ccgatggttt tgccttgcaa ttctcgtccc
    12421 acataagcac ttcttttgtt ttctgcctgt tctaaaatat taggacctgt tagtgtttgt
    12481 accatacgac ttgcttcgat tatcggacgg gaagataaga gcaaacagca caaaacaagc
    12541 tcttttactg cattagcatt tacacctggt gtattcatga cgatcgtccc gttttcagaa
    12601 gctttttcaa cgttgatcgt gttcacccca acaccagctc ttgaaattgc tagcaaatct
    12661 ggtgtaagcc attcatcttt cactttcgta ctacgaataa ctattccttc ggctggcaaa
    12721 tcatctatta ttttgaaata ttccccttgt aacttttcta tctcttgttc tggaatagca
    12781 tcaatcaaat gaacgtgata cataaaaccc cctccttact aacaagtatc tcgaaatttc
    12841 tgcaattaga caactgttta ccatttgact tcctagaagc aaaaagagca gttagagcag
    12901 agtaggctga gtgataaaaa taaggctatg acaagttact gccataagcc ttattttcga
    12961 gagcaaaata ttgagggtga gatatcttga taaatggtgt tccgttagct gacattgata
    13021 ataagactaa atttcaaaaa agttgaaaag caaatttcgt caatctcttc ttatttctta
    13081 aaactaagtg gctttttcac aacctctttt tatctaataa ccactgtttc caactcaact
    13141 aattttgccc acgtttcaat ttgatctgtt gttagattta gcgagacaac ggtatgatga
    13201 ccgccacctg cctgaatcca tgaatggaca ccttcatgga aattcggttt agttttccat
    13261 aacacacgtg caacaggtaa attaggtgct tcagtcgttg gttcaaaggc ttctacttca
    13321 ttgatcagca gtttataatg cgtaccgaaa tctgccatcg atacgactac cccttctcct
    13381 gctttcccat caaatacaag acgcgccgga tcttctctat ctcccattga taaaggagaa
    13441 acaacgattc ttggtttcgt cgcagcaagc gtcggatcta cttccatcat atgggattcc
    13501 aagattgctt cttggccact agctagctcg taagtataat cttccatgaa tcctgtatct
    13561 ttccctcgag ccatgatttt cagcaaccga tcaatcgctg cagttttcca gtcgccttcc
    13621 ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag ctagtccagg tagttgtttc
    13681 attccataca ggtcttcaaa attagtcgta aaagcgttat agcctccttc ttctaagaaa
    13741 cggcgaatac cgatttcctg ctgagcttgt acttttacat gttcctccca tgtcttttct
    13801 tcataatcac catagtcgaa ggtatacaat tccttgtact cttcaaataa ttgatcgact
    13861 tcttcgtctg atacgcgatc gattacttgt acaagatctc cgataccaaa ataatcaaca
    13921 gtccaaccaa attgaatctg tgcttccact ttgtctcctt ctgtgacacc cacgtttctc
    13981 atgttgtcac caaaacgagc cacttttatt ccaaaacttt cattataggc tactgcaaca
    14041 tccatccaat cagcaatctc tttttggatc tcaggtcgtt tccagtagcc aacgacgatt
    14101 ttattttgtt tatttaatcg agcattgatg aacccatatt cgcggtcacc atgagcggat
    14161 tgatttaaat tcatgaaatc catatcgatc gttttccaag gaatgctttc gttatattgc
    14221 gtagctaaat gaagcaatgg tttttgtaga agctttgtcc cgcggatcca catttttgct
    14281 ggtgagaaag tatgcatcca agtgatcaca cctgctactt cttctcgata attgacctct
    14341 ttcatgattt ttgtgatcgt atcagcagta actgctaatt cttgaaggcg gattggataa
    14401 ggcaattggc cattttcatt cagttcatcg accatttctt gtgcatgttt ctttacttct
    14461 cgcagtgctt cttccccata taaatgctgt gatcctacaa caaaccaaaa ttctttttct
    14521 ccaatattca acataattag ccactccttt tatttgtttt gtccgtaata cgcatttttt
    14581 ccgtgttttc gtaaataatg tttatctaaa atccgttgag gcagctcttc tgaaaatgaa
    14641 ttgagctgtc tggtaaataa attcatcttg catacttcat ctagtacgac tgcgttcatt
    14701 accgcacttt gcgcatcttt cccccaagta aatggaccgt gtccgtgtaa aagtacccct
    14761 ggaattgcta agggatcaat tttgcgttct ttgaatgttt ctatgataac ttttcctgtt
    14821 tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa atcgagcaca aggtaccgat
    14881 ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa catccaatcc tgcttgtgcc
    14941 cagatcgtcg cccatgtcga gtgagtatgg acgattcctc cgatttgagg aaatgaacga
    15001 tataaaacag catgagtagg cgtatccgaa gagggattga gtttaccttc aatgatatga
    15061 ttgtccaagt ccacaacgac catgtcgtct gctgtcatct cttcataact gactccgcta
    15121 ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc tcacatttcc ccatgtatat
    15181 ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt atacttcttc tttcagttgt
    15241 tccagcattt tctatcactc ctttttcttg caattgatga atcgctgttt tttcaatgtc
    15301 gatcccctca cggtaacgtc tcataaatat ctgatagcct gctatttctt ctgctgaagg
    15361 tgtgatggtg attccttggt cttgtccaaa cacgtcttct gccaagaagt tttctaaaga
    15421 cttcccatgt cgatccatca tataggctgc taataacgcg attccccacg ctccaccttc
    15481 tccggctgtg tccatcactg taactggtgc ctccatagca gatgctagaa tccgctgtcc
    15541 aacttctggt gttttaaaaa tccccccatg ccccactaaa cgatcaattt ggacattctc
    15601 agatttcaat atatccattc cgattctcat tgcaccaaaa gcactggcta aatggatccg
    15661 catgaaattc gctaaatcaa aacggctacc tggtgtacgt acaaataatg gccttccttc
    15721 atttactcct gtaatgtttt caccggaaaa ataaccataa gaaagtaatc ccccgcaatc
    15781 cgtatcgcct tctagcgctt tgttgaataa taccgtgaat aatttatctc tgcttatctc
    15841 catacccaaa gaattggtaa attcttcaaa aatactgacc caagcattga tatccgaaga
    15901 gcagttattc gtatgaacca tgcctactag ttctcctgaa ggtgtagtga ccatgtcgat
    15961 ttccggatgg acattttcta gttctttttc taatacgatc atagcgaagg cggaagtacc
    16021 agccgaaatg ttccctgtcc ctttggctac actgttcgta gcgaccatac cggttcctgc
    16081 atccccttct ggcggacaaa ccgggattcc tggtctcaaa tttcccgtag ggtcgatcag
    16141 ataagcccct gattcgctta acttgccagc ttcttctcct gctactagaa ctttaggcaa
    16201 aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt aactgaccaa aaatattttt
    16261 catttcttga tggtaggttt tcgttgaagg atcgatagga aacatgcctg atgcatctcc
    16321 tacgcctagt actttctcac cagtaagctg ccaatggaca taacctgcaa gcgttgtcaa
    16381 aaaattaatt tctttcacat gttcttcttt attcaaaata gattgataca aatgagcgat
    16441 actccatctc tgcggaatat tgtattgaaa tttttccgtt aatattcgtt ctgcttcttc
    16501 agtgattgta tttcgccaag tacgaaatgg tactagtaac tgttccttgc tatcaaatgc
    16561 tagataaccg tgcatcattg cactgaaccc aatcgacccg attgtcgtaa gattctcgcc
    16621 atagatttcg aatacttctg ctgccatttt tcggtaactg acgcgcagtc ctttccaaat
    16681 atcttctaat gaataggtcc agataccatt ttctagtttg ttttcccact cataactacc
    16741 agcggcaata ggagtaaaag atgaatcaat caatacagct ttgatcctag ttgatccaaa
    16801 ctcaatccct aaagatgtac gaccatctat gacatctaac atactttgcg catttacttc
    16861 cttcataatg atttaactcc ctccttctcg tcaaacctga tcaaaccgtc tttttagtag
    16921 cgcttacaat gattattgta tacttttgta cgtacatgtc aagcagttat ttacaagaat
    16981 acaataaaaa acacttgttt tgtacgtaca taagttataa aatgaaattg cttacaaaat
    17041 ctgatcaacc gccttctcga aattctagaa ggagagaata cttatgaaaa aagaaaaaaa
    17101 agtttcctca tcgtttattt atttttttgg cgcatttggc ggcattctat ttggatatga
    17161 tattggtgtt atgaccggcg ccttaccttt tttacagcat gattggggtt tggcaggtaa
    17221 agcttctttg ataggatgga tcacgtcttc ggtcatgtta ggtgcgatcc ttggcggctc
    17281 tttatctggt cttctttctg ataagcttgg acgacgaaaa atgattcttc tttctgcact
    17341 tatttttatg gctggttctg tcctttctgc atctgcccct cacaatggtt cttattttct
    17401 gattgcagca aggattcttc taggtttagc tgttggcgct gcatctgccc tagttcctgc
    17461 ttatatgtct gaaatggctc ctgctagact aaggggaaga ttgtcaggga tcaatcaagt
    17521 gatgattgct tctggcatgc tcctttccta tgttgcagat tatttgctaa aagggttgcc
    17581 tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct gtccccgcgc tgattttatt
    17641 tttcggtgtt ttagctctac cagagtcacc gcgttttctt atgcaatctg gccgcttgga
    17701 agaggcaaaa agagtattga actatattcg gacaccaaaa gaagctgaac aggaattcga
    17761 acaaatccag ctgaatgtca aacaagaaaa gacaaccgga acttcttggc atactttgtt
    17821 tttagaaaaa tatcgttcct tagtcattgc cggtattggt gtagcagtat tccagcaatt
    17881 tcaaggagct aatgctattt tttactatat tccgctgatc gtagaaaaag cgactgggca
    17941 tgcagcgagc gatgcattga tgtggccgat catccaagga atcattttag ttgctggttc
    18001 tttgctcttt ttggttattg cagacaagtt caatcggcga actttattga aaataggcgg
    18061 tagcgtgatg ggactttctt tcatcctacc agctgtttta ggaactgtac tggatgctca
    18121 tacaaattcg ttgttgattc tcttgttttt atgtatttat gtcgcatttt attcttgcac
    18181 atgggctcca ctgacatggg taatcgtagg cgaaatattc ccactagctg tccgcgggcg
    18241 agcatctggc ctagcctctt cttttaattg gattggttct ttcctagttg gcttgctttt
    18301 tcctgtgatg actgcttcta tgtctcaaga aatcgttttc ggcatttttg ggatcatctg
    18361 ctttttaggg gttttattta ttcaagagat cgtaccagaa actcgtggga aatctttaga
    18421 ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa cgtatttcta tccatcattc
    18481 ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata agaaggaacc gcttagtttc
    18541 cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt tgatgaatat tgtgacttgt
    18601 gaccagttgg taggagaaca ccgtttagtg gagaggtggt gaaagaaacg ttcagccttg
    18661 agcgatgaca aaagctctca caaacattgc gacgagtaac cgtaggagca ataagaggcg
    18721 gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt tcccgttcaa ctagtacagg
    18781 ttcatagaga atatttggct gtgcctgtcc tttttcaatc gttgtgacga tccaccgtgc
    18841 ggcatcttgt cccaactttt cttttggatg cgtcaatgtt gtcaattttt tattccctaa
    18901 atggcttaaa gaagaatcgt ctgttcctac gatcgatagg tcatctggaa tatgatagcc
    18961 attttctgtc aacttatcta gcattaaact agctatttga tcgttatagc aaatgatgcc
    19021 atctaaagct tgttcttctt tcaaccgttg tactgttttt tcagcgattg tttctcgtgt
    19081 ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc acattatact gttcacacgc
    19141 gctgataaaa cctttcatcc tgtattttcc ttgaagatcg tcgatttttg tgatgaacaa
    19201 cagacgctga tgtccttttt ttatcaaata ttctgctgcc ataaaaccga ctttcacatc
    19261 atctaaacaa atataagagg catttaactc ttcataagca gcattgatca tcaccatcgg
    19321 aatgccttgt tctctcaaag caacataaag tgctaagttt gggttatact gattactttt
    19381 cgtaggctca acgatcaaac catctacccc ataagcaatc attttcttca aacactcttt
    19441 ctccaattcg tgatcgttat tcgtactggc taaaagcaat gaatacccag attctcgtaa
    19501 ttcattttcg attcctcgga taatcgacgg aaaaatataa tcagagagat atgtggtgat
    19561 gacaccgatc gtttttgttc tgttcaatgg tgttttttct ttcttatagc tgtcatctac
    19621 atatgtgccc gacccttttt ctttacgcag atagccctca tttaccaaca cagcgattgc
    19681 ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc agcttgaatt ctgagggaat
    19741 ggttgtattt acttcgtatt cctcacgaag gatcttttgt tttatctctt ctgcaatatc
    19801 ttgatatttt gccatttgcc ttgccccttt ttatttgtac gtacatatat cataacgagt
    19861 ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc tcataaggca tacggaacag
    19921 gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa gcattgcgcg ttcgcgtgcc
    19981 gattaggatt gtctaacgga tccccttttt ggttcttctc tggtcgagca tgatagatca
    20041 aaatatcaaa gcacccgtct tctgaaaccg taaacgagtt gtgaccagga ccaaattgct
    20101 tatttttttc agaagaaacg aatactggct ccgataattt atgccatgat tcttttaata
    20161 aaagattctt gtgctcatca gcccacagca ggcccatggc atagttttca tctgttgcac
    20221 taccagaata cgtaatgaat attttcccat tgcgagtaag gactgctgga ccttcattta
    20281 ctgaaaaacc ccgtttttcc cattcatact caggtataga aaggaggatc tgcttcccct
    20341 ttaacgtcca aggattatcc atttcggata gataaagatt agaattccca gtaatttcag
    20401 gatcttgttg ggcccacaca taatatagct ttccttgaga agagaaagtt gttccgtcta
    20461 aactgaaagt ttcccttttc gtttttattt gtcccttttc tatccaatct gtattcattg
    20521 gatctatatg ctgattttca agtacgaaca tgcggtgatg atgagattca tcacgtatag
    20581 ttttatgatt gcttgcagca aagtaaatat accattttcc attgatataa tggacttcag
    20641 gtgcccagat caattcactc atcggtcctt cttcatgagc ctgccataca atcaggcttg
    20701 cagcatgttc taattcattc agtgactttg cccttcttaa ttcaatcact tggtagcctg
    20761 gaaccgatcc tgtaaaataa taataaccat ctgtatgttt atatacccaa ggatctgccc
    20821 gttgccgaac gataggatta ttataaagtg tcttcatcat aacaaatttc ctcacatttt
    20881 ctttattcta attacattcc aagagtattt ttctatccct acatttaatt cgtcatgttt
    20941 ttctattgaa aaatagttta tttcaggttt tattttttct ggttcttcac gtgtattttt
    21001 gtccaacaga tcgctactgg ttaatgtgta gtgctctcct tgatctagca aattgaaatc
    21061 atgttcaatc gttatctttt gttgttcatt tgtgcgattg atcaagaaaa tagtcagctg
    21121 attgccgtta tctacgatca cactgtccac ataaggaaca ttttttatct tttcacaatc
    21181 ataaacaggg caaatgattt gttgtttcaa tacttgacct ttagcatatt tcgataagtg
    21241 ttgcatcaca taataagttg gattcctcca tgcttgtcct ttagcatctg tcagtattaa
    21301 aggtattgtg tttactaaca atgattgaca actaatcttt acgcgatctg cgtgtcgtaa
    21361 aatagccagc cctaatgaac caaataataa tgtatcttcc atagtaaagt aacaccaatc
    21421 gatgggacta ccgacttcaa aatccctatg tcttttttcc ggttgggcat gtacattcca
    21481 ttcatcaaac gctagataca ttgttttctc acttctcaaa tatcctttta cataatcaca
    21541 agtagcaacg atttcttcaa tttgacgatc gaatcgataa gcttttgcaa ggtatgtcgc
    21601 tgtatcatca cgctcatttt taggaggttt agtcaagtct tcttcatcat acttatcaat
    21661 atagttatgc aaagctaaat aatctacatt ctcataagct tcttctaatg cgatacgatc
    21721 ccattcaggg tacgatggta aacgtggcgt agagcttcca actaagattg tttcaattga
    21781 atcatccact aacttcatga cttttgaggc ttcatttgct aacctaccat attcataagc
    21841 actcttagcc cccacttgcc atggaccgtc tagctcattc cctaaacacc ataatttaac
    21901 gccaaaaggc tttttttgcc cattttttat tcttaaatca ctccagaagg ttccttttgg
    21961 aaaattacaa tattctacta aattttttgc ttcatcaatt ccttttgttc ctaaattcac
    22021 cgtgaatatc ggtttcgctt gtacatgact gatccattta aaaaattcgt ggagtccaaa
    22081 agaatttggt tctatacctc gccatgctaa atcaattttt tttggtcgtt gttctttcct
    22141 acctacagta tccacccaat cataaccaga tgtaaagttt cctccaggat agcgaatgac
    22201 acctaattct aattccttta cataagagag gacatcttgt ctaaacccgt caccatcagc
    22261 tgtttcatgg cccggttcat aaatccctgt atagacgaca cttcccatat gctcaacaaa
    22321 tgagccaaac agccgagaac tgatctctcc taccgtaaaa tcctctctga tttttacttt
    22381 cacttcttcg tccaacagct ttccctacct ttcatttcct atagtcaaga taatagatac
    22441 aagtttccat acgccaccag caaagataaa aataaaagga gggaaaagaa atatgagcag
    22501 tacaaatcct aataacaata cgcaacaaag aatatttctc aaaaaatcaa aaaatacttt
    22561 ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct ttgttttcag aaatcacata
    22621 tatccaggat aaaagaaaca tggaataaat aagtaaaact acatatccac taattgaaag
    22681 tacgattgtc tgttcttcta ataattgatt aacaagaaaa taaataatga tcgttaacag
    22741 actaaggaac aagttcttac caaaatgttg ctttattcct tgaaagaaaa aataaatgat
    22801 atttcctgat ccctcacctt tccattgcgc cattacataa aaaacagcac atgtagcagg
    22861 cacgatagta ataacaggca aacacacgat tacccagaga atacctaata agatgtaatc
    22921 agaaaccaca atcaactgtc cataaagttt agtctcaact attttcatct tcctcactct
    22981 ttcattccag acatagctgt tccttgaaca aaatatttct gtgcaaaaat ataaaccact
    23041 agtaatggca gcattgaaat aaccgttcca gccattacag gtccataaaa agaaatgttc
    23101 tgccctgtaa ataaagctaa ccctgctggt aaagttctca tttttgaact acttgtcaat
    23161 atcaatggat aaagcaagtc attccaacta ttcatcagcg tgaaaatccc taatgataaa
    23221 agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa tattaaactc acttaatcca
    23281 tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa taaaaaagga acgcatcatg
    23341 aagataccaa atgcagttgt catacgagga atgatcaaac ctgcataagt atcaagcatg
    23401 ccaagcaaat tgacttcaat aaatagagga atcatgaaga cttggaatgg gatcatcatt
    23461 gtcagcaaaa cgaaatagaa caaaactgat tttcctttga aacgcatacg agcaaatgca
    23521 taccccgcaa gagaatcaaa gaatacagaa gtgatcactg tccctccagc aaaaatcact
    23581 gtatttttga tgtaatctaa taaagggatc gttgtccaaa ccttaatata gttatctagg
    23641 gtatagctat ctgcaaatat tctaggcgga aatgagataa tgtctttttc gtgtttaaaa
    23701 gaagaaataa tcagccacaa aaaaggaaac gcaataacca acaatataac tagcatacta
    23761 atcgttcgaa tgattttttt accactaact gcttttcgac tattcacagt aactgtaatc
    23821 gagctttctg gtgccataca ggtttccctc ctatttcatt tgtttttctt tccgtgacat
    23881 gtacacatta gtggagatag ctatagttgc aacaatgata aacaaaaccg aagacagtgc
    23941 tgatgcatag cccaattcat aaggtgctgt aaatcctcga ctgtaaatat attgaacagc
    24001 tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca tatacttggt caaacacttg
    24061 catcgaacca atcagattcg ttaaaataca aaaacctaag gagggaacga tctgaggtag
    24121 cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc atttgagcag attcatacaa
    24181 agagggagag attccctgaa tagcagtcaa gagaatcgtc atagtaacac caaaattctt
    24241 ccaaacagtc atgatggcta ccgtcggcat agcaagggtt ggatcgcgaa aaaattgagg
    24301 gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc cctacattcg gatcaagcaa
    24361 catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata actggaatgt aaaatgtcga
    24421 acgacaaaaa cgattgaatc tggtattttt agataatgct actgctgcta ataaaccaac
    24481 gataatttgt gttggcgttt cgagtacggt gaaatataaa gaatgaatca acgaattaac
    24541 tgctcgttca tcactaaata tacgtgtgaa attttcaaaa cttacaaaat ttcttgatgt
    24601 aaagaaaata ttcaaatctg taaaactaat agcaaatgtt cctaaaagcg gtatgacagt
    24661 aaataataat aaaactaaaa aagatggagt taaaaacaaa tacgcagcac gatcaggacg
    24721 attccaatat ttggtgaatc cgttcatata gtccacctca tctttcattt tgataaaata
    24781 tacaatcttg gtttaaacga atctgaataa gatatccgaa caatcgctgt gatttttgca
    24841 ataacaccta tctgcaaata ctaggtcgat cattcagata tctgtatctt gccaacagtc
    24901 tatcctgtat aaagttctgt actatttttg cagtaattga ttgatggcat catctgcatc
    24961 gttcatgagt gtctcaggat cttctcctgc taataatttt tcaatcagtg gattgatgat
    25021 atcgttattg atcgcagaga tttgggtaaa acctggtaaa tatggctctg catagtccat
    25081 ttgtttcgat aattcagata caataggatc attttttact tcttcatctt ttgctactga
    25141 atccaagtag gcaggaaaac cattttcgat actccatttt ttaccaattt cagttgtatt
    25201 ccaatatttc aaaaactcat agacagcttc ttttttatta ttatctgtac ttgccggaat
    25261 ggcatatcct acgccatcta aaatagctgt ttttttgtct gaatcactcc cttgtggtaa
    25321 ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg ttatttaacc atggcccatt
    25381 gatttcaatg gctagttgac cagcattcat taaattgtcc atttcggcgc cagaaatcga
    25441 ttctggacca gtcttgtcta catggatcat tttttggatc gcttttaacg ttttaaggtt
    25501 ctcaggagac gcaaattcag acttagtata atcatcgttc actaatttac cgccatttgc
    25561 taacatccag ttatataacg gtgcagttcc atccttattg aagacaaaac ctgatacatt
    25621 tttactacta tcagttaatt ttggtgccat ttctgtcaat tcttcccaag tagtaggtgg
    25681 ttgttcagta tctaatccag cttgtttaaa caaatcatta ttccaataca tatacatgcc
    25741 ttgtacctgc atggggacaa aatactgttt accttctact tcacctaatt caatagccgt
    25801 ttctttaaaa tcagatttat ctactccatc atattcccag aaatcgtcta atggttgtac
    25861 cgctccgttt tctacatact gtgcaaaatc tggataattt aatgcaataa agtcaggtgc
    25921 ttttttagaa gaaatcgctg taggaagttt ttcattcaaa ttggcccaag tcatgatatc
    25981 catttcaatt ttgatgtttt tatcatttgt tttattaaat tcattaacga tttcttgcaa
    26041 tatttcacca tcagatgcag taaacccatt ccaaaaagtc aaagtcactt ctttatcaga
    26101 atcagattct gctgaacttc cgccaccgca agctcctaaa atacctgttg cagctaccaa
    26161 caaagagcca attaaccatt ttttaccttt cattttttgt tcctccttca tctgataaac
    26221 gacatctgac cacattccaa gatagcggtt tcaataaaca attgattttc ccatcttcag
    26281 cttttacatt tgttgaatga cttggtttta cttgttcact tttaggaaga ttagttgctt
    26341 ttgtatcaaa tccactcatt tcagaaaaat caatcactcc ctctaatgat aattcagaca
    26401 agtgaacagt tagattcatt tcttcttctc cacggttaac tgcaaatatg acaagttctt
    26461 tgtcatttat tacggctata ctttctaaat acggcacttc ttcaaaatct tccgtcgcat
    26521 agtttgctga acgaatttgt gggcttaaca cctctccttg tccatacaca gcagcttgca
    26581 taaatggata atagatcgtc tgtctccaag cttctccgtt tgtttctgtc ctgattggtg
    26641 caataacatt taccaattga gcaagacaag caatctttac tctatctgca tgtttcaata
    26701 aagtgattaa tagacagcct aatagtaatg catcttcaaa attatagtta tcttctagta
    26761 aaggaggagc aatctgccaa ggcgctgctt gctcatcttg ttccgctgaa tgataccaaa
    26821 tattccattc atcaaaggac aggttgatct gcttcttact atgtttcttc gccttgactg
    26881 tatcgcacat cgctgtcact tcagaaatga accgatccat gtctaaggaa cgtgcaagat
    26941 aatgagaaag atcatttttt tggttgccat aatattgatg cagagacaaa taatctattt
    27001 gatcgtacgc ttgatcaaga actgttaact cccaatcacc aaatgtaggc atttgccgat
    27061 tcgaacttcc gcataaaacc acctcaattg aatcatctac gagtttcatc gccttagccg
    27121 tttcctctgc taaccgtcca tattcttccg ctgttttatg tccaatctgc caaggaccgt
    27181 ccatttcatt ccctaaacac catgtcttaa ttccaaaagg gctctcttga ccattcgaac
    27241 gccttaaatc actccaataa gttcctttag gaaaattaca atattcgact aaattacgag
    27301 ctgcatctac tccacgagta cccagattga cagccatatt gatttcggcc cccacatgct
    27361 gacaccagtt ataaaattca tgaatcccaa cttcattcgt ttcaattgtc cgccaagcta
    27421 aatccaacct tctaggtcta tttgctatgg gcccaatact gtcttcccaa cgaaatcctg
    27481 acacaaaatt tccaccagga tatctaatca tgggaatgtt caattctttt accaagtgaa
    27541 tgacgtcttg gcgaaatcca cttttattag ctgatggatg atctggctga taaatccctt
    27601 catatactgc tcgtcccata tgttcaataa atgaactgta caaccgcgga tcaactttgc
    27661 ctacacgctt gacactacta atcaacattg tggcttccat aactgacctc ctttaattta
    27721 tacatgaata atacacctcg ttgaaagcgt tgtcaataat ataaatgtta aacttactta
    27781 ttttttatcg attttaatta accaaaatat taataatgat taaaacaaat aatttaatat
    27841 aacttttatt aacaaatatg ttaatatata agaaaaacct tttaagaggt gatatatatg
    27901 caactacaaa taaacgaaga atccttaccg gtatatgaag cattagctag taaaacaagg
    27961 atcaagatca tccagctact ttccaaaaaa aagatgaatg tcaaagattt ggcaaaagaa
    28021 cttggagtga gcagtgctat cacgacgatg cacgtgaaaa agctagagga agcaaacatc
    28081 atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc ttcgtgtcga taaaatcgat
    28141 atcagttttc cagaaaaaat attcaatgct tttgatacga aagaaacatc gatacctatt
    28201 ggtcactata caaattatgc aattgaacca acatgcggtt tagctacgat ccatgatttt
    28261 atcgggaaag tggatgaacc acgctatttt atggatcctc gaaggatgga tgcccgcatt
    28321 ctctggttta ccagcggatt tgtcgagtat caggcaccta attttttaaa tacagaggat
    28381 acattggaga tgttggaagt ttctgtggaa atttcttctg aatttccttt ttcaaatgac
    28441 aattggcctt cagatatcac tttcagccta aatggtgtgg agctaggaac atggacgagc
    28501 cctggagatt ttgctgatat ccgcggtaaa tatacgcctg attggtatcc agataatctt
    28561 aatcaatacg gcttgctgaa gacgatccga attaccaaac acttgacgaa tatgaatgga
    28621 gaacccttgt caaacatcac gatcaacgac atccctaaag agcaagacac ttggcatttg
    28681 cgaatcgaag tgaaagacga tgccaaacat gtggggggct gtactttatt tggtaaagga
    28741 ttcggtaatt acgatcagga tatcaaatta aaagtttact acagctgata tttttctttt
    28801 ctattttata aaacaaaaac agccaatccc tatgaaggga cggctgtttt tgctttattg
    28861 ttatttccag aaatcatcca aaacggaaat tggcaaatgt cgtttatgtt gtgtcttgtt
    28921 ccaccatgct tcgatttttt cttgtgcttg agcgggaact tcttttcctt ctagataatc
    28981 atcaatttca ttatatgtga cacctagtgc gacttcatct gcaatcattg gtttcccatc
    29041 ttcaagatct gcagtcggaa tttttgtgta taatttttcc ggtgcattca atgcttgaag
    29101 caattgtttc ccttgtcgtt tgtccaaacg gaataatggc aaaatgtcgg ctccaccatc
    29161 accaaatttc gtgaagaatc cagtaatgtt ttctgcagca tgatctgtcc ctaatacggc
    29221 tcctgctctt tctccagcta cggcatattg cgtaatcata cgttgacgtg ctttgatatt
    29281 tcctttgtta aaatcgctga ttcccacacc tgcttcgctt aatacttgta cttgtgcatc
    29341 aacagctggt ttgatattta ctcgcagact aacatcgggc tggataaagt caagagcagc
    29401 ttttgcatct tcttcatctg cttgttcacc ataaggaaga cgaacagcga taaattgata
    29461 gttttcatct tttgtttctt cacgcatttc ttccatcgtt agttgcgcca aacggcctgc
    29521 taaagtagaa tcttggccgc cgctgatacc tagtacaaaa gttttaagaa acggatattt
    29581 tttcaaatat gctttcataa agtcaatact tttacgtatt tcttcttttg gatcaattgt
    29641 tggtttgaca cctaattcat tgataatttc tgtctgcagg ctcatttatt ctcctcctat
    29701 gcttctgtat gaagttctgc tacgcttttt ttcattcgtt ccatgatagc cattttatgg
    29761 ttccagcatt cagtagacag atcgactggg tatttttgcg gattcaagtc tcgtttatac
    29821 tcttcccata gtgaatctag attttctttt gtatattctt tgatttcgtt tagtgcaggt
    29881 aattcgtaaa ctctttttcc ttcgataaag atatcttgta aaactgggcg agctgtaaag
    29941 tctcgaacag ttttattgat aaacgtatgc actggatgga acatgaagat agcttcttct
    30001 tctcgagggt cttcatccca taatgtcacg tagtctcctt cagatttccc atcaaaattt
    30061 cgagtgatgc gccacacttg ttttttccct ggagtagtta ctttttctgc attgcttgat
    30121 aacttgatcg tatccatcat ttttccttca tcatcttcga tcgatactag cttgaatact
    30181 gcaccaaggg ctggctgatc ataggctgtg atcaattttg tcccaacacc ccatacatca
    30241 attttagctt tttgcatctt taaattcaaa atcgttgctt catccaagtc attcgaagca
    30301 tagatttttg cttccgtaaa tccggctgca tctaattgtt cccgaacacg cttagatata
    30361 taggccatat ctccactgtc gatgcgtacg ccttggaagt tgattttgtc acctaattct
    30421 ttcgccacgc gaatcgcact tggcacacct gatttcaacg tatcataagt gtcgactaag
    30481 aacacgcagt ctttatgggt tttagcgtag gccataaatg cttcataatc atttccataa
    30541 gattggacta acgaatgagc atgggtcccg ctagctggta tcccaaatat cttcccagca
    30601 cgtacattgc ttgttgcatc tgctccgccg atatatgccg ctctagttcc ccagactgct
    30661 gcatccagtt cttgtgcccg tctagttcca aattccaata gtggatcttc tccgatgaca
    30721 gatttgatac gagctgcttt tgtcgcgatc aacgtttgga aattgaccat attcaacagt
    30781 gctgtttcta ctaattggca gtgcgctaaa ggcccttcca cttggattaa tggttcattt
    30841 gcaaaaacta gttctccttc acgagcagaa cgaactgttg ctttaaattc aaaattttgt
    30901 aaatactcca aaaaaccttc aggatagact tctaatccac gcaagtaagc aatatcggaa
    30961 tcattgaacg tcagattctc taaataattg actaaccgtt ccaatcctgc aaaaaccgcg
    31021 tatccgtggt taaaaggcat ttctcgaaag taacattcaa aaacagcatg taaatcagct
    31081 cgacctaatt cccagtatgt ctgcatcata ttgatttgat atagatccgt atgcagcgtc
    31141 aaactgtcgt ctgggtaagt attattcatc ttgtattact cctcttggca agttttcatt
    31201 tcctacccat tataacaaaa aaaattaata ggaaaagaaa tcttccattt tttctgaaaa
    31261 agcacatgtt ttacggatat ttcttgctaa aaatgaactc catctgctaa aactatcctt
    31321 tcttattggt atttaaccta aacgagaaag aatacttatt ttattcaatg tatccgagac
    31381 acgaagaaat tagcgttttc tatttttctt acttgataaa ataagtagat tttcctacta
    31441 caatcgtagc cgttaagata ccgatgattg aacaaatata accaaatgtc tctacaacat
    31501 aatcaatggc aaaagttttt gaagcaataa tacgtctttc cttttttgct tcttatcccc
    31561 aaagaaacgg atataactta ttatgtattg aatttttttg gaaaggcgaa ttatgacaag
    31621 atataccttg cgttgaaaaa actcgcggaa aaaatatcac acgtttttaa aaagatacaa
    31681 agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg cgagacatca atcagtctcg
    31741 ccttgttttt tattgtatac attagatatg gagcaggtta ccataaagat gattattagt
    31801 cgttattaaa tagaatcctt cgtatttttt tgttctatca ctacaatatc tttatttgga
    31861 cgtttgaatt gtattccggc acgttctagc tcctgtttca ctcctacatg gactttgaca
    31921 tcttctcgtg tcatttttaa aacgtgatga atcgcatagt cgatttcttg ccttgtgatt
    31981 tttttcttcg tattgatcat cacgacttgc tttttatcat atgtgtctac caatagcagt
    32041 tgtgttcgat ctaacgaaaa taatttgtaa taattaatag ccgtattccc aaaaaagaat
    32101 tccactactt taggataaaa tgtcttaata tttaaattac tattcacagg tgtttcgatt
    32161 agcttcatct cattcctctt ttctatatag tcgatttaga tccaatctta agttatgtta
    32221 ccatctttcc tagcattaag gaatcggtta caattgaacc tttaaagaca aaaggattta
    32281 ccttttatca cttaatagta taacataatt tcgtgagaaa aaaatacaat tttttcttga
    32341 ccatctcgtt acgtaaagga ttatgcttac atttgtcagg aggaaaaata atggagtaca
    32401 caataaagaa aatgtcagag atatcaggag tcagtccgag aaccttgcgc ttttatgatg
    32461 agattggact gctaaaacca gctaggatca attcttcagg ttatcggatt tatggcaaaa
    32521 aagaagtcga tcgtctgcaa cacatcttat tttatcgaac aatggcattc aagttggatg
    32581 atatccaaga ggtcttagac aatccatctt ttgaccacca gaaagcttta atcaagcatc
    32641 agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct cttgacaact gtccaacaga
    32701 cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa agaaaaattt gaagggttta
    32761 aacaacaaaa gttgaaagaa aatgaagaat cctatggaca agagattcga caaaaatacg
    32821 gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa tatggataaa gaaacgtatg
    32881 aagaaatgca aactgttgaa aagcagatgt tatttgatct cactctttac ttaaaagaac
    32941 cagaagatga aagcttagca gaccgtatct tccaagctca taagaagtgg ttctcttaca
    33001 gctggcctga atatcagcca aaagcgcata aaggactagg aatgatgtac gaagccgatg
    33061 aacggttcac tgcatattat gacgagcgaa gtggtcatgg tgcagcaaaa gctttaaatg
    33121 aaattatcca aaaaaaggcg taatctttta catgatccaa caaaaaagta cacgtaaaat
    33181 gacctaataa caaaacagca gacacccaag ttagattttt atgctaactt ttggtgtctg
    33241 ctccattctt tcacaagttt tttcatccta ctttaaatat attcatcgtt gaataaacgt
    33301 ataaggtatt ttcactttat cctttttttc tgtaagaaac aattgaaatg ccatttctcc
    33361 gcaagcttcc agatggtggt cgatggttgg gaaaccaaga agctcgctga tcagcaaatt
    33421 atcgcgtcca atcaccactg gtgtcttatc ttgcagatag ctttgcaaga tgcctgctgc
    33481 aaccatatcc gaatttgtaa agacaccatc aactccaagc tctttaaaaa agaagcctgc
    33541 ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca agtgattcat caaaagatgg
    33601 aaaaagttga tgacatagct gcaaagtgat ttttgagtta taacttagcc tgccacttcg
    33661 ccccaacgtg atgcccaatt gctttatacc ttgctgcttc aaataagtaa gaccttctgt
    33721 aattgattgt tctcgatcaa taaagacaca gcttgcctgt tttcccttga tttcttcaca
    33781 aaaaactaga ggtccatatt tttgatatgc caataaatca tctagtgtat tggcacgaga
    33841 cgtaatgatc aatccatcga aagctttagc tgcaaattct tcaagatacc ttcgctcttt
    33901 ttctttatga tagcccgtcg gtaaaagtgt cactttgtaa tcataatcaa acgctgcttc
    33961 aatgatccca ctgatcaact gatcaaaata cggatgattg gtataaggca gaatcactcc
    34021 aatgcttttc gtttttccat aacttaactg tctggcattt gcattaggga tataatcgac
    34081 ttctttcatg accgcaagta tttctttcct tttttcctca tctacatatg gatgtccatt
    34141 gattacacgt gaaatagtag aaactgaata acctgtgagc tttgcgatat ctcgaatgtt
    34201 tgccactgct gcttcgccac ctatacaaaa agattttctt gtaaatagtc taccacagct
    34261 ggaccgcatt caaaaaactg atacattttt ccttcatgcg aaaaactgat ccgctgcccg
    34321 ccccaatata tatcttttgt caaattttcg atttcattca ttggtatttc ttttactata
    34381 ttcctattca acccttgaaa actgtacaac gttaatacgg atcccttgaa ctcgacaaac
    34441 gcataaaaat aattgttcgt tgtgccatct gttatctcta cataagtatt cattgcaagc
    34501 cctccttttg atactttgag tatagtgtat gaaacgcgtc tcatagcaag gagtttgttt
    34561 tgccaaagaa aagttttatt cctctttcat tattaccaaa gtctcttatt ttaaattgaa
    34621 tacattataa tgatactgta aatatatctt tattattagt gaatg
//
LOCUS       DAHHPA010000020        33867 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.021, whole genome shotgun sequence.
ACCESSION   DAHHPA010000020 DAHHPA010000000
VERSION     DAHHPA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.021"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            128..1093
                     /locus_tag="NBH55_000870"
     CDS             128..1093
                     /locus_tag="NBH55_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCD1508875.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1150..1380)
                     /locus_tag="NBH55_000871"
     CDS             complement(1150..1380)
                     /locus_tag="NBH55_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCD1508876.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1466..2353)
                     /locus_tag="NBH55_000872"
     CDS             complement(1466..2353)
                     /locus_tag="NBH55_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1508877.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTNTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2442..3665)
                     /locus_tag="NBH55_000873"
     CDS             complement(2442..3665)
                     /locus_tag="NBH55_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCD1508878.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3682..4728)
                     /locus_tag="NBH55_000874"
     CDS             complement(3682..4728)
                     /locus_tag="NBH55_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCD1508879.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4892..6619)
                     /gene="ptsP"
                     /locus_tag="NBH55_000875"
     CDS             complement(4892..6619)
                     /gene="ptsP"
                     /locus_tag="NBH55_000875"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCD1508880.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6619..6885)
                     /locus_tag="NBH55_000876"
     CDS             complement(6619..6885)
                     /locus_tag="NBH55_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCD1508881.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7364..9598
                     /locus_tag="NBH55_000877"
     CDS             7364..9598
                     /locus_tag="NBH55_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1508882.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9746..10066
                     /locus_tag="NBH55_000878"
     CDS             9746..10066
                     /locus_tag="NBH55_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCD1508883.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLSV"
     gene            complement(10271..10494)
                     /locus_tag="NBH55_000879"
                     /pseudo
     CDS             complement(10271..10494)
                     /locus_tag="NBH55_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(10692..12272)
                     /locus_tag="NBH55_000880"
     CDS             complement(10692..12272)
                     /locus_tag="NBH55_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCD1508884.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12673..14049)
                     /locus_tag="NBH55_000881"
     CDS             complement(12673..14049)
                     /locus_tag="NBH55_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD1508885.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14177..15295)
                     /locus_tag="NBH55_000882"
     CDS             complement(14177..15295)
                     /locus_tag="NBH55_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1508886.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15437..15871
                     /locus_tag="NBH55_000883"
     CDS             15437..15871
                     /locus_tag="NBH55_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1508887.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15949..16482)
                     /locus_tag="NBH55_000884"
     CDS             complement(15949..16482)
                     /locus_tag="NBH55_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCD1508888.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16576..17754)
                     /gene="mutY"
                     /locus_tag="NBH55_000885"
     CDS             complement(16576..17754)
                     /gene="mutY"
                     /locus_tag="NBH55_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCD1508889.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17747..18544)
                     /gene="recX"
                     /locus_tag="NBH55_000886"
     CDS             complement(17747..18544)
                     /gene="recX"
                     /locus_tag="NBH55_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCD1508890.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18651..20033
                     /gene="rlmD"
                     /locus_tag="NBH55_000887"
     CDS             18651..20033
                     /gene="rlmD"
                     /locus_tag="NBH55_000887"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCD1508891.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20207..20785)
                     /locus_tag="NBH55_000888"
     CDS             complement(20207..20785)
                     /locus_tag="NBH55_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCD1508892.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21107..21577
                     /locus_tag="NBH55_000889"
     CDS             21107..21577
                     /locus_tag="NBH55_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCD1508893.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21685..22911)
                     /locus_tag="NBH55_000890"
     CDS             complement(21685..22911)
                     /locus_tag="NBH55_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD1508894.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23665..23865)
                     /locus_tag="NBH55_000891"
     CDS             complement(23665..23865)
                     /locus_tag="NBH55_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1508895.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23999..24607)
                     /locus_tag="NBH55_000892"
     CDS             complement(23999..24607)
                     /locus_tag="NBH55_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCD1508896.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24692..25162)
                     /locus_tag="NBH55_000893"
     CDS             complement(24692..25162)
                     /locus_tag="NBH55_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCD1508897.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25210..26124)
                     /locus_tag="NBH55_000894"
     CDS             complement(25210..26124)
                     /locus_tag="NBH55_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD1508898.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26270..27073
                     /locus_tag="NBH55_000895"
     CDS             26270..27073
                     /locus_tag="NBH55_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCD1508899.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27251..28198
                     /locus_tag="NBH55_000896"
     CDS             27251..28198
                     /locus_tag="NBH55_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD1508900.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28266..28559)
                     /locus_tag="NBH55_000897"
     CDS             complement(28266..28559)
                     /locus_tag="NBH55_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCD1508901.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(28587..29252)
                     /locus_tag="NBH55_000898"
     CDS             complement(28587..29252)
                     /locus_tag="NBH55_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCD1508902.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29392..30024)
                     /locus_tag="NBH55_000899"
     CDS             complement(29392..30024)
                     /locus_tag="NBH55_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1508903.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(30031..31098)
                     /locus_tag="NBH55_000900"
     CDS             complement(30031..31098)
                     /locus_tag="NBH55_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1508904.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31095..31826)
                     /locus_tag="NBH55_000901"
     CDS             complement(31095..31826)
                     /locus_tag="NBH55_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCD1508905.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(31995..32474)
                     /gene="greA"
                     /locus_tag="NBH55_000902"
     CDS             complement(31995..32474)
                     /gene="greA"
                     /locus_tag="NBH55_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCD1508906.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32610..33785)
                     /gene="mltG"
                     /locus_tag="NBH55_000903"
     CDS             complement(32610..33785)
                     /gene="mltG"
                     /locus_tag="NBH55_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCD1508907.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT        10105 a         6871 c         5608 g        11283 t
ORIGIN      
        1 tcatctatct cacatatttt gattatcaaa gtattttctg tgaacagaac tatagaattc
       61 cctaaaactt gtccattatt tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg
      121 agatcctatg ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg tctatcttat
      181 gaaagaccga gatggtaagg tcatctatgt tggtaaagca aaacggctca aaaatagagt
      241 aagcagctat tttcatcaga ataaacagca ttcaaaaaaa gtattacgta tgattcatca
      301 tataacagat tttgatttcg tcgtagtaga cacggaatta gatgctttat tgctcgagtg
      361 tcaattgatc caacattacc gtccttttta taaccgacaa atgaattatt tcagtaatta
      421 taattatgta catattacca ataagggatt tgtattaaca gatactccta cggctcgaac
      481 atacgggcct tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac
      541 ttatcaaatg ccttggttgt ctgaaattag tttgttagct ttgcgagtcc agcttcctga
      601 cctacaggaa atgacttttg aacagaaaaa aaaggaactc caagggctgt ttcaaggccg
      661 aaataaaaag ctactgactt acttgaaaaa aagacaacaa catttcatct atcagttgaa
      721 tttcgaaaaa gccggtatgc ttcaaaaaga tatcgaatta gttacttatt ttattagacg
      781 gatccaggaa caaaagcagt ttttaagaac accatcgctc accttcagca tgccattagc
      841 agcagatgag agtcagaaaa agcactacct cgtttgttat ggtcaacttg cagaaacaat
      901 gattacctct ggtgacgttt ctcctgactt ctattatgaa aagaaagaag ctcatttatc
      961 tttgaaacgt caattgtcaa aagaagaaat cgatcctgta caaatcttga tcagttaccg
     1021 caaaaaatta gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa
     1081 gcaactaaat tagttgcttc tcggcttctt ttaggcatct tcaacttcaa atgtcaatta
     1141 tcctaaaatt tattggatta agtcatagat ttccatcgct acgatatcga tattatcaaa
     1201 ttcgtattgc tgatgagtat ctgtttcagt taattcaaat gtatcggtcg ctttatcata
     1261 agttactgta cacttctcta cgccttcttt ttcaaaacgg cgaacttgca gttcattatc
     1321 tgctgactcg accatagcct ccagtcgttt tatgattgcg actaattgtg aatgtgtcat
     1381 cggaggtcct ccttttctta tttctgttat attgtatcaa aagcatagcc tacttgcatt
     1441 gattttctct aaaaaaactc gttttttatg attttttttc tggtattttt ccttcaggcc
     1501 accaaagttt gatcaaagct tgggttccgt cattctcaaa acctttatct gccaactctt
     1561 catatagttt caatgcttgg gtagttgctg gcaatacaag atccattttt tctgcttctt
     1621 ctaatgcaat ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt
     1681 cttttaaaat acgaggagaa taattgctta aagaccagtt agctgcactt ccacctgaaa
     1741 gcgtctcaat cactttcttt aaatcaagtc cgtttttttg tgcatagaca agcatttccg
     1801 tcattcctgt catcgtacct gcaatcatga tctgattagc catttttgta tgctgacctt
     1861 tcccagcaga accatgcaat gtaaatgtcg taccaaattc tttgaataaa ggcagtactt
     1921 gatcataaac ggcttcttct ccaccgacca tgatcgtcag tgtaccattc tttgctccta
     1981 agtcaccacc ggataccggt gcatctaacg catctgcgcc tttttctttt gctgtttttg
     2041 caattttttc tgccaggctt ggtgtatttg tagtcaaatc aactaaaata tgtcctgaga
     2101 tatctgcaga aaaaatcccc gactgtccat agtagatttc ttctacatct ttggggtaac
     2161 ctaccattgt aaagagaata ttactttgtt ctgctaccgc ttttggtgta tctgcccaga
     2221 tagctccttc tgttactaaa ggatcagctt ttgcttttgt ccgattatag acagctactt
     2281 cgtgtcctgc ttctagtaga tggcgtgcga cagcacttcc catgacgcct gttccgataa
     2341 aaccaagttt cattttttcg acctcctaaa aatcaaaaat ccttgcttct atgctagtat
     2401 acgctagaaa caaggatttt ttcataaaaa atgtttgaat tttatctttt aaatgaattt
     2461 aattttattt tgattcgttc aatcgagtct tcttcttctt ttcttcgcat ctcttcttca
     2521 tgataaacaa ccatgtcttg ataaaattcg atcatagctt cgccgaaatg tgtagatgaa
     2581 atatcataaa gtttagcttc taaaactttt ggatctttag gaacttcact ggaaatataa
     2641 tcaataagtg ctggtgcaaa gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga
     2701 tcgaacaatc ggttcaaata agcatttcct tccacgacac aaggtgttcc ggctgccatc
     2761 gcttctgtat aagtaagccc ttgtgtttct gaagtagaag cactgacaaa ataatctgcc
     2821 gctttataat aaagtgctac atcattgttg tccacttctc ctgtgaactg cacaaattct
     2881 tctaactgca gttctgagat caattcctct aactcttcta cataaggtcc ttttcctacg
     2941 ataactaatc tggcgttagg ataagcggag atgacctgag gaaacccatt aatcaaggct
     3001 tggatatttt tctcataaga aatcctgctt aaagaaagaa tcatcaaatg gttttcctga
     3061 agacctagtg actcccttaa tttgctgata tcctgctgag tgatatcaga acgtttgaat
     3121 ttttcaatgt cgataccagt aggaatcacg cgaagcggtg ctattacacc atagctgcgt
     3181 aatgtatcaa taacacgttc acttggacaa actacacctg tcgtatgatt tgcaaataat
     3241 ctagacaagt attttacatg ggattgcctg atcacttttc ctttaccgat ataatgaagg
     3301 tagtcttcat acatcgtatg gtaagtatgg atcagaggta ttttcaactt cttagctacc
     3361 atcttcccaa gtatccctgc tccgaattct gtatgggtat gaataagatc tagttctagt
     3421 tctttggcaa ccaaataagc ataccacatg cctctaacaa caactcttcg atccttgaaa
     3481 gaaataaatg gtacacttgg cattcgaatc acatcttttt ccaacttatc cgcattagga
     3541 tcggtcgttg taaaaatata gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt
     3601 ttgatcgatg tagcaactcc gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt
     3661 ttcatgtttt tcgcctccaa actagtcctt gacctgattt acttttttta atttgctaga
     3721 gactttttca ttcagtacac tttcgtatac atttttaagt tcttcaccga ttttattgat
     3781 acttcgctct tttgctgttt gataaccggc ttgtgtcgta ctcggtagtt cttgattggt
     3841 aatctgttgg atcagtcgga taaattcttc gtttgtcttc cccatatagc agttcttgct
     3901 atcttctaac cagccattat acactggaat atctcgaaca agtacgtttt gtttgcttgc
     3961 taaagcttct aaaacaacga tcccttctgt ctcttcataa gaagggaaaa agaatagatc
     4021 tgcacctgaa tacgcacctt ctatgatctc tccacgaatg taaccaggaa acaggacatt
     4081 ttcaggatga tcttctttta caattttccg gatgttccga ggaatcgagt acatgggaac
     4141 atgaccaaac caaataaatg tgtattcggg catcttctta gcaatttcta cgaaatcaat
     4201 gattccttta cgttcgaaaa acaaaccgac acaaatgatt actttttgat ccggagacag
     4261 tttgaaatat tctctgaact tttgttcttt cattggatca gggtgatatt tctctaagtc
     4321 gatcccattg gaaatagctt gaatcggcac acggatacca tagctttcca acaactgttt
     4381 tgaataaggt gttggcgtga tcagatagtc cgctttttgg tacaatccta ccagatattt
     4441 ttttacgata ggagaaagct ggttcgatcc gataaaggaa tttcggaaat cttcctctgt
     4501 agaatgcgca tgatagacaa ctttttttcc ttctcttctg gctttattga ccatattatg
     4561 actattgatt ccgtatgtat tgatgtgcag gatgtcataa tcttcttttt catctagtgt
     4621 ataagagata ccgacttccg tcagagcacg tttttgatga tcaagcgcac gcccgattcc
     4681 agattttgca agcatctttt cactttcaaa atataataat accttcacct tacgtccccc
     4741 ttcacttata tagtataaca tagcctacaa actgtcactc aaacttaagc cctatttttg
     4801 tctattctta ctgacaaaaa cgttttctta aaaaagaaca aaaaaactgg cgtagctctt
     4861 ttacaagcca caccagcttt tgatacgttg tttattttac atattcatga acaagttcaa
     4921 cgacttcttc catcatatcg cattctttca atgcacgatc agcaagttca gccattttcg
     4981 ctgtgtccaa gcgtttcatt aaactacgcg ttttaagaat agatgtcgca ctcattgaga
     5041 actcatctag acccatacct actagcaatg gaacagctgt ttgatctcca gccatttctc
     5101 cacacatacc tgcccatttt ccttcagcgt gtgctgcatc aattacattt ttgatcaaac
     5161 gtaaaattga tgggttatat ggttggtaca agtaagaaac gcgttcgttc atacggtctg
     5221 ctgccattgt gtattgaatc aaatcgttcg tacctacaga gaagaaatct acttcttttg
     5281 caaacttgtc tgctaagact gctgctgctg gaatctcgat catgatgccg acttggatcg
     5341 tatcagatac ttccttgcct tcacttatca atttttgttt ttcttcttca aagatcgctt
     5401 ttgctgcgcg gaattctttg agtgtagcta ccatagggaa catgatacgt aaattaccat
     5461 gaacagatgc acgaagcaat gcacgcattt gtgtacggaa catttcgtcg ccttgttcag
     5521 ataaactgat acgcaatgca cgatatccta agaatggatt catttcatga ggtaattgca
     5581 gatatggtag ttctttatct ccaccgatgt ccattgtacg aacaactaca ggttttcctt
     5641 ccataccttc taatacagct ttgtaagctt catattggtc atcttctgtt gggaagtctg
     5701 gagaatccat atacaggaat tcagtacggt acagaccaac tgcttctcca ccattattat
     5761 ggacaccaac tagatctttt ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt
     5821 cagctgtgac agtttctgca ttttttaatt tttcccattc tgctttttgt gctgcgtaat
     5881 ctgcacctgc tttttcaaat tcagcttttt gttcatctgt aggatcgatg attacatcgc
     5941 cttcgatccc gttgacagcc aaaatatcgc cttctttgac tttagcagta atttcttttg
     6001 tcccaacgat tgctggaatt tctaaagaac gtgccatgat agcagaatgt gaagtacgtc
     6061 caccaatgtc tgttacaaac gcttttacaa aattgcgatc taattgtgct gtatcacttg
     6121 gagtcaagtc atgtgcaaca acgaccactt cttcgttgat cattgatgga tttggtaaag
     6181 taacgcctaa taagtgcgct aaaatacgtt tagcaacgtc acgaatatct gcagcacgct
     6241 cttgcatata agcattatct tccattgctt cgaacatacc gatatacata tccgttactt
     6301 cttttagcgc tgattcagca tttacgctgt tgtctttaat attttgcttg atttgaccta
     6361 ccatttctgg atctgaaaga accatcaaat gggcatcaaa tacttgcgct tctgcttcac
     6421 ctaagctttg cgctgctttt tcacgaattt gctgcaattc ttcagtagat ttagctaatg
     6481 cagcatccaa acgagcttct tcagccgcag tatcttctac tgttgcctta ctgaatgtta
     6541 aatccggttg aactagcagg taagcttttg caacggctac accgtcacta gcggcaatcc
     6601 cttttagcat ttcaaccatt attcagataa gccttctttc ttcattgttt caacgattgc
     6661 tgccattgca tcagcttcgt cagcaccttc tgcagtaata gtaacatcag aaccttgacc
     6721 tacacctaaa gacataacgc ccatgattga tttcaagttt actgatttac ctttatactc
     6781 taagtttaca tcagagttaa atttgcttgc tgtttgtact aatagtgtag ctggacgagc
     6841 gtggatccca gtttctgcta ctacgtgaaa ttctttcttt tccatattaa tcggtctcct
     6901 ttaagtgagt gttgttttta tgtgagggtt ttctttttta gtaattcaga gaatcctaac
     6961 aaatgccacc ctttcatctg taaagattaa catttttttt cgttagatac aacagttttc
     7021 attcaatttc agataaaagt attcaaatta ttccggattc tttttcatcc ttttcttcac
     7081 attttataac aaacgtgttt atttcctttt tcttgtcgct ttctcttctc ctgacgttta
     7141 tcattctttc ctttgaccct ctttgttttc atatgaaacg aattacttgc ttcccttttt
     7201 atttttagta taataagagt caaaggtcag gaatggtcaa tagagatttc accttttcta
     7261 cctcataaat ctcttaaaaa actaggggtt attcttttac ctcatgaaaa acgagatgta
     7321 aaatatggat gtacccattt ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg
     7381 cggcaagaat gaggcaacga ttcacttgta tgccactgtc aatggtcagc ggacacaact
     7441 cgattactgt cagagctgtt atcaaaaaat caaaaatcaa caaaatggag gtctgactaa
     7501 tatggcccaa aatgatccat ttggatttgg cagtttggac gatcttttcc gttccatgtc
     7561 tcgccaaatg caacagcaag gaaattatga gcaaacacca ccaacacaat ttggcggaaa
     7621 caacaacttc aatggtggtc agccacctca aggcaataca gaaggtttat tgggagaata
     7681 cggaatcaat atcacagaac aagcacgaca aggtgatatc gatcctgtga tcggacgcga
     7741 tgacgaaatc aaacgagtca ttgaaatttt aaatcgccga accaagaaca atcctgtctt
     7801 gattggagaa ccaggtgttg gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt
     7861 agatggtgat gtaccgcaaa aattaatgga caaagaagtc attcgtttag atgtcgtatc
     7921 tttagtccaa ggtactggta tacgaggaca atttgaagaa cgtatgcaaa aattgattga
     7981 agagatccgt caagcagaaa atgtgatcct attcatagat gaagttcacg aaatcgtcgg
     8041 tgctggttct gctggtgatg gaaatatgga tgctggtaat attttaaaac cagctttagc
     8101 tcgaggagaa ttgcaaatgg ttggtgctac taccttaaat gagtaccgca tcatcgaaaa
     8161 agacgctgct ttagaacgtc gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga
     8221 aacgatcagt attttgaaag gtttgcaaaa acgttatgaa gactatcatc atgtaaaata
     8281 tactgatgag gccatcgaag cagccgcaac tttatccaat cgttacgtcc aagatcgctt
     8341 cttgccggat aaagccatcg atttgctaga tgaaacaggt tctaagaaaa acttgacgat
     8401 ccaaatcgtt gatcctaaaa caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt
     8461 tttagcttct cgagaagaag atttcgaaaa agcagcttat tatcgcgatc agatcaataa
     8521 acttcaaaaa atgaaagaac gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa
     8581 agatatggaa aaaatcgttg aacaacggac aggtattcca gtgggtgaat taaaagaaaa
     8641 agaacagaca caactgaaaa acttagcaga tgacttgaaa gctcatgtca tcggacaaga
     8701 taacgctgta gatcgagtag cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca
     8761 gaatcgtcca atcggctcct tcttatttgt tggaccaact ggtgtaggta aaacagagct
     8821 tgcgaaacaa cttgcctacg aactattcgg ttcacaagat tcaatgattc gtttcgatat
     8881 gtctgaatat atggaaaaac atagtgtctc taaattgatt ggttctcctc caggttatgt
     8941 cggatacgaa gaagctggtc aattaacaga aaaagtacgg agaaatcctt acagtcttgt
     9001 cttgttagat gaagtagaaa aagctcatcc agatgttttg catatgttct tgcaaatctt
     9061 agatgatggt cgattgacag atgcacaagg aagaacggtc agctttaaag atacgatcat
     9121 cattatgaca agtaatgccg gaactggtaa agtagaagca aacgttggtt ttggtgcggc
     9181 acgcgaaggt gttactcgtt ctgtattgaa ccaattaaac aactacttta caccagaatt
     9241 tctgaaccgt tttgacggga tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac
     9301 aatcgtcaca ctaatgctag acgatgtcaa ccaaatgctg gcagcacagc aacttcacat
     9361 cgaagttcct acgaatgtca aagaaaaatt agttgactta ggctatgatc catctatggg
     9421 tgctcgtcct cttcgacgca cgatccaaga acaaatcgaa gatggtatcg ctgaatttta
     9481 tcttgatcac ccttctatcc atgagttgaa agcaaaactg gataaagatg gaaaaatcat
     9541 cgttacttct aaaccagaac gtttggctaa agaatcagcc gaagaaacgg ctgaataaat
     9601 ccagttttat agcgtgagac ggtatttctt tgagaaatgc cgtctcattc ttttttcaga
     9661 aaagtcaaaa tactgttcat tttataaaaa agaaattata ataagagaaa caagcaatta
     9721 aaaatacata aaagaggtga gcgccatgaa attggtaaat gtaacaaaca gccattcccg
     9781 tcttgtatta aaccagcttg aaaacactga tgcccatcta gtcaaggtct acacagccgg
     9841 aaataccact attgtctaca ctgaagcccc tgaacataat gaaattctgc tgatcaatga
     9901 caaacgtaaa atccagccga aagaaatcga agatgctaaa aattatttct tagataaaat
     9961 caaagacgcc gtttaccacg aggatgaaat caaagtaatc gaattggcag gccttgttga
    10021 aatctcgatt ccaaaacatg aattaggcgc tacattatcc gtgtaaacaa attttgaaca
    10081 taataattaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
    10141 tataaacggc gaaagcagaa gcaactcctt cggaaataag ctgaaattca cgaaaattgc
    10201 ttttcaaatt tttgtgaatt ccagcttatt tcttggagtt aaacgcttct gtctcagcct
    10261 ctttttcttt ctagcttttt gatttttctc ttttcttttg tacttttctc ttttcatttt
    10321 cagttctacg cgtgtcttta tttttaactt tttcctcttt atggatgatt gatcgtactt
    10381 tttgtgcaat ttgctcgttt ttgtcccagt cttttacatc taataatcga cataatcgta
    10441 acatctcttc tttttctgtt tttttctttc acgtctttgc cattctgttt ccatgtgtca
    10501 cactctcctt tctttaatca tatgtacgca aaataatttt tatatttttt tgattagaca
    10561 tttctatgat tttaatatag taaaaagagc cgtcacggaa aatccgtcac ggctctaaac
    10621 aaaatatgag aggctgtttt tgtttggcta ttcttattac ttgatgcagg gcagcttgtg
    10681 tcaagacttc tttacatcaa actttttaat tttacattgg gatatttatc cgcaaaccag
    10741 cgttctgcaa attggttctc gaataagaac aacggttggt cgaaacggtc acgagccaag
    10801 atattgcgac ttgaactcat cttctcatcc aaatcttctg gatcgatcca gcgagcgatt
    10861 ttgtgtccca ttggagacat gattacttcg gcgttatact catttaacat acgatattgg
    10921 aatacttcga attgtagctg accaacagct ccaatgatat attcttccgt tagatatgtt
    10981 ttataaagct gaatcgctcc ttcttgaact aattgataga ttcctttgtg gaaggatttt
    11041 tgtttcatga cattttttgc agtcactttc ataaacagct ctggagtaaa tgaagggagt
    11101 tcttcataag caacttttag ttttccttcg aacaatgtat ccccaatctg ataattgcct
    11161 gtatcatata caccgatgat atcgcctgct actgcttttt cgacgttttc tctcgaatct
    11221 gccataaatt gtgtgacatt gcttaatttc agttttttat ttgtacgttc caaccagaca
    11281 tccattcctc gttcgaatgt tccagaacag attcggacaa aagcgattcg gtcacgatgg
    11341 gctggattca tatttgcttg gatcttgaat acgaaacctg aaaattcttc ctcataagga
    11401 cttaccatct gatcatcttg tgttttgtgt ccataaggag ctggtgccaa ttcaacgaaa
    11461 gtctctaaaa aagtctgtac accgaagtta gtcaatgcag aaccaaaaaa tactggtgtc
    11521 tgatcgccac gagcaatttt ttcttgatta aatgcatctc cagcctcttt gattagttct
    11581 acttcttcta agacttgttc atataaggaa ttttcagtta gaggatgatt ttcgggaagc
    11641 agtccatctt catccaattc tactaatcgt tcatcctgat aattttccgg acgataaaac
    11701 tctacacgat tatggtagat atcataaaga ccttctagcc ctttacccat accgattggc
    11761 caattcatcg gataagattc gatatctaat agctcttcta attcttctaa caattccaat
    11821 ggttcacgac catcacgatc tagtttgttg ataaaagtaa atatcgggat gccacgtttt
    11881 tttacaactt ggaataattt ctttgtctgt gcttcgatcc cttttgcact atcgattacc
    11941 atgaccgcac tatccacggc catcaaagta cgataagtat cttcagaaaa atcttcatgt
    12001 cctggtgtat ccaagatatt tactcgtttc ccttgataat caaactgcat aacagaacta
    12061 gtaacagaaa tcccacgttg tttctcgatt tccatccagt ctgatttggc aaaattacct
    12121 gtttttttgc cttttacggt cccggcttga cggatagccc caccaaacaa tagtaattgt
    12181 tctgtaatcg ttgttttccc agcatccgga tgggaaatga tcgcaaaagt tctgcgattg
    12241 tccacttgtt gctttaattt tggattattc ataacctaac tccatttctt ccattcaatt
    12301 atttatattt tctctaggca cagattgtcc gatctcttaa agaaagacaa tttcatatag
    12361 ggctgtgaca aaagccgtgt agcagtctct ttttccgagt aaacggcgtt ctagagacag
    12421 cctatcagca ataagcccac ccaagccaaa aatacgaaaa gctgtttttg cttggctgct
    12481 cttattactt aatgcaggat agctttttca caacctttgt caactactac aactgaatca
    12541 gtatagcatg gattcagcga atgaaaaagc caccaaacca taagagtcaa atattttttt
    12601 gtcagctgac taaaagaaaa gacgtgttgc ttgtttattc aagcaacacg tctttcataa
    12661 ccaagtttcc aattatcttc ttggctcgtc atcttcgaat tcttttctga agtatcttcg
    12721 ttcttcttca ggttccgctt ctgtttcttc cggatcatga aaaatgacac ggattttaag
    12781 gattcgagaa ccttccattt cttcagaaac cagcgtaata ttatcgacat caaacgataa
    12841 cttttcgcct tcatccggaa tcatgcctaa tgctgtaatc aaataaccag ccatcgtatc
    12901 tacatcactc atatgcagat cactgtcaaa agcttcattg aactcatcaa tcagcatacg
    12961 tccttggatg atatattcgt gttcatcgat tttttcataa agattctcaa cttcatctga
    13021 ttcatcatcg atttctccga cgatttcttc taaaaggtct tctagtgttg ctaacccaac
    13081 gacgccgcca tattcatcta ataaaatagc catctgattc tgcgtctttt tcatttcata
    13141 taacagatca tcaataaaga ccgtttcagg aacgaataaa ggctcctgca tgattttctt
    13201 gatatctaaa ttatcaaagc cgaatttatg ggcagctttt agtaaattct ttgtatgaag
    13261 gattccgact actttatctt tgtcttcgtt ataaacaggg attcttgaat agttttcgga
    13321 taaaacttca ttcacatttt cttccacagt gtcgttgata tcaatcataa atgcatccgt
    13381 tcgtgggacc atgacttctc gggcaacttt cgtatcaaga gaaaaaaccc cttggagcat
    13441 ttctaattct tcattattca atacgccttc gttttcaagc atataacgca tttcatctct
    13501 ggtcattttt gaatcttcat catcaaaagt catcggcgtg atacgcgcca ataaacttgt
    13561 ggaagcagaa agcagccaga cgaagggctt agctactaca ccaatcaagc gtatcagccc
    13621 tgaagtgaac tttgcgactt cttctgattt atttaaagca atacgtttag ggtacaattc
    13681 tccaaaaaca atcgatacat aggtcaaaat agccagcaca atgatatttg caatattttt
    13741 tgccgctgtg ccgccaccta ataccggagc taaccgttcc gataaagtat tagctagtga
    13801 agcaccagac aaaatgttga ccaaagtgat tcctacttgg attgtcgata ggaaattatt
    13861 tggattctgc aatattttta aaagcttttg agatttcaca tctccttcat cagctttttg
    13921 ctcgattcga ttcttattga tcgaaacaac agcaatttcg gaagctgcta aaaaagcatt
    13981 gatcaatgtc aaaacgatca ataataaaat ctgcgctaac agcgactgac tctcagggtc
    14041 agcattcata atcgcattct ctccttataa aattaatttt tagtttacga tagaaaaaaa
    14101 cgtggcacta tagccacgtt aaaatggtat cacaaagcaa ttttctttgc aataaaaaca
    14161 agtgtactaa aactacctac gaggtcgtag caattttctt cttgttttca ttttgcttgt
    14221 cttctttgac gattttgatt atataggaaa tgatcgtctt gcagattgcg taaaaaggaa
    14281 cacctaagaa gataccaagc aatccggcaa tatttccagc tactagaaga acgataatga
    14341 tcgttaatgg atggattttc agtgatttcc caattacatt aggataaatg atattcccat
    14401 ccaactgttg gacgattagc acaaccacac aacaaagtaa tgcttttacc ggatcattga
    14461 agactgttac tagaaatgca ggtgctagtc ctagataagg tcctaaatat ggaattaggt
    14521 ttgtcaatcc cgcaatgacc ccaaataaga aagcataatc cacaccaatt accatatatc
    14581 ctaaaaatgt gaaggtacca acgaataaac attcgattgc ttgtccgcta atgtaattag
    14641 ccaatgtttt attcagttgc cccaataact ccacaatttg gtttcttctt ttttctggga
    14701 aaaactgttg gatgtttggc actagttttt ctccgtcttt caacatatag aacaagatga
    14761 atggtgctgt aatgattact acggttgccg aagcgatcgt tccaacgatc gaacctaaac
    14821 tattggatac tccactaagg aattgctgaa tgattgtccc gtaagaaatg tctaattgct
    14881 caaaatattt attgatatct acttccttga acaaaggcaa ttcggctacc tcttttaacc
    14941 agctttctac ctgtttgatg aaatcaggca tattagatgc caatgaggaa atttgctgta
    15001 ctagacttgg gatgacacta agtaaaatcc aaataattgc tgcaactaaa aggagcagca
    15061 caattgcgac agcccatatt cttttgattt ttgctttcat caacagcttg acaataggat
    15121 tcaataaata gtaaagaaag cctgcaatca gtacaggtgc aaataacgtg gaaaaaaatg
    15181 tcccgatcgg ttcgaaaata aagtttattt ctgatgaaat catgatcaat gcagctatga
    15241 tcaataattc aagcgaccaa aacattagtt tagactgtct aagtttttca aacaatagat
    15301 ttcctccttt ctttcttact agtatatatt atattcgtta aaaattcttt tattcttttt
    15361 ttatttttga aaacttgcca tactttttat ttgttttatt ttccagtata ctatacgaaa
    15421 ggaaggtggc tagaccatga aagttgtaca aacaaaagat acaatgagca atatttactt
    15481 agatgctgta cggatcaggc atcaagtttt tgtagtcgaa cagggtgtgc ctttgtctcg
    15541 agaaatagac aaagatgaag cccattgtat ccacttcgtc ctgtactcag ataaaaaaga
    15601 accgcaagga actgtacgtc ttttgccttt agaaaacgga aaaatgaaat tacagcggat
    15661 ggcgattctt tctgaataca gacaccaagg gttaggaaaa atattgattg aagaagcaga
    15721 aaactttgct aagaatcaag gctataatac gattttatta ggggcacagt ccacagctga
    15781 aactttttac gaaaaactag gatatacagc ttacggcgat ccttttgaag atgctggcat
    15841 gcctcatatt tatatgaaaa agactttata aaaatttatt tcgaaaaaag caaaaaacag
    15901 caagcttttg agaaaaatct ctcactggct tactgttctt ttttgtgttt acttacgcga
    15961 caattgcttg taacggttat accaaatatc aatatagcct tccgagaacg ggccatctcc
    16021 attattgatc cagtcaacca gaatcttcac attttctttt aggatgaagt caatatcgtt
    16081 cgggtaattc atcattttgc tgtgcatctc atattcatcg acatccaata gtcgtttctc
    16141 gccgtcagga aataccttga tatccaagtc atagtcgata tatttcaagg cctcatcgtc
    16201 cagcaaaaaa ggtgaagcta gattacaata ataggaaacc cccttttctc tgatcattgc
    16261 tattacattg aaccagtatt ttttatgaaa ataaacaatc gctggttcgc gcgttaccca
    16321 tcggcgtcca tcagattcag tgactaatgt gtgatcattc acgccgatca tagagtgttc
    16381 gcttgttttt aataccatgg tatctcgcca cgttcgatgc aatttgccgt cgtgcttgta
    16441 actttggatc gtcacaaact ctccttcttt tggaactccc atagcaaccc ttccttaagc
    16501 agcattttgt gctttgtgtt tgggtgttca ttacaccgac atcataatta ttatatcaat
    16561 aaaaatgagc tttgcctaga aaggattcct attgtttcga tttttctcta actgatctac
    16621 cagtttcatt tggaccttag gaaaaacgac cgaatcaaat gcatcaggct tcaaccactt
    16681 cgaagttttg ttctcggtaa attcttgttc tgctccctcg gttgcccgtc cgtaaaaaag
    16741 gagaacatgc catttcagat gactaaaaat atgagtaact tctcctaaat gtcgtgtctg
    16801 ccaaacaaag agttcctgct tttcaaatgg gagattttgc tcttcttctg ctactaggtc
    16861 atcaaacaga tcgatttctg gctttgtttc ccattctttt ttcaatcgct catactcctc
    16921 ttgtgtcact tccatcattg gaaatgtcca catattcgcc agcaattttt ggctatctct
    16981 ttcttcaaaa taatacgctc cagaatgatt ttgaagagct gcactgatat aataaacgtc
    17041 ttttggcttt gctttttttg tttttacagg aaaactcgtt tggattcctc gtttgttagc
    17101 taaacaaaag gcttggatcg gacaagtctc acactttgga gaagttggtg tacagatagc
    17161 tgaacctaga tccatcattg cttggttgaa ttcacctgga tgcttttcat caatgatttt
    17221 ccgcatcgct tcatcaaaga ttttcctgct agaagctttt gcgatgtctg cttcaatgca
    17281 aaacaatctg ctgactactc gcatcacgtt gccgtcaact gcaggttctg gaagtccaaa
    17341 tgcgatactt gcaatcgcgc ctgtcgtata tggtcctatt cccttcaatg agctaatttc
    17401 ttcaggcgtt tgcggcattt ccccatcaaa ttctgacatg atctgtttag cagcagcttg
    17461 gatgttccgg gctcttgagt aataaccaag gccctcccat gcttttaaaa gcttttcttc
    17521 tggagcattt gctagctctt caattgtagg aaaccattcc atgaaacgat aaaaataatc
    17581 gatcactgtg tctactctgg tttgctgaag catgatctca gagatccaga tacgataagg
    17641 atcacgatta tagcgccaag gcaggtttct tttttcttgt tcataccatt gaataaactg
    17701 atcttgaaat tcttttgttt ctttatctgt ccatttttcc cattctttat tcactttcct
    17761 cttccttttc cgtaatgaat cgctggatca tatccaaatc aaatcctttt tgatataagc
    17821 tttgctttac ttttagattt ctttttttag gttcaaacct taaatttctt cgccatagtt
    17881 tatctccttg tttgactaaa taatcatatt ctatttcttg atctgcttct tgcggaagat
    17941 cagctatcgc ttgttggatc acttcttgag aaaagccttt cgtcatcaat gtttgttgga
    18001 tttttctcaa taattctttt tggctttttc cgtgattttt tccaaacgct ttttcggctg
    18061 tctgatgtgc attttgaact tggtcttcaa atgtatattg gctgagcgct tgttcaatga
    18121 tttcaggttt caaacctttt tgctgcatct gctgcgctaa tttctttggt cctttgtcac
    18181 ttaagcgcat attcgtgcgc acataacttt ccccatagac caaatcatcg acaaggtcca
    18241 attcttttaa gcgggctacg attttatttc gatcttcttg aaggatttcc ttttctttta
    18301 agtatgttcg gatttctttt tccgatcgca actgataact taagtaattc attgctaggt
    18361 gaaaaccgaa atcagctccg ctactcttct tgatctcttg gatagtttct tcgtctaatt
    18421 cccgtccttt taataatcga tagcgtacta atacatcttc agatacttgt aaagcttctc
    18481 cttcagataa atctacgcga tagaaagggc ctttgccttt actgattctc gtaacggtta
    18541 acataaacat cctcctcgaa catatattct ctatcatacc acagaaaatc cccgtaatca
    18601 ggtattatat gttagaataa accacagaaa aaagaaaaag ggagatactt atggcagaaa
    18661 caattgtcaa aactggacaa acggttcctt tgaaaatcaa gcgtttaggc ataaatggtg
    18721 aaggaatcgg ttatttcaaa cggttgatca tatttgttcc atatgcacta ccaaaagaag
    18781 aagtactagt gaaaatcacg aaagcaactc ctcgctatgc agaaggagaa ttaataaaag
    18841 tcaaaaaaac aagcaaagat cgcgtagtgg caccatgtcc tgtttactat gaatgcggtg
    18901 gctgtcaatt acaacactta gcttatcaag ctcaattgga ttttaaaaag gatctgcttt
    18961 tgcaagcatt ggaaaaattc aaaccagcag gctttcgatc ttatcaactt ttgccgacga
    19021 tcgggatgga tgagccttgg cattatagaa acaaagcaca atttcagtta cgaaaaaata
    19081 agcagactca aaaaattgaa gctggtttat acgaagcaaa ctctcatcag ttagtcccta
    19141 taacagactg cttggttcaa gaacctcgaa cacaaaaagt aatgaatact gttgttcaac
    19201 tattgaataa atttgatgca cccatctatg atgaacggac aaacagtgga atcttccgca
    19261 caatcatggt tagaatcggt gttaaaactg gggagttaca agttgttttt attactcgca
    19321 gtaaaaaatt cccgcaaaaa aacgcaatga tccgtgagat caatgaacga ctgcccgaag
    19381 ttgtttcaat catgcaaaat gtccaggcac aaaaaacgtc tctcgtgatg ggggatgaaa
    19441 cgatccactt atggggaaaa gaagccattg aagaacaaat caatgaagtg actttcgatc
    19501 tttctcctcg tgccttcttt caactcaatc ctcagcagac cgaattgctt tatggcgaag
    19561 gaatcaaagc attagatgtc cagcctaatg aaacgatcgt ggatgcttat tgcggagttg
    19621 ggacgatcgg cttaagtgta gcaaaacaag ccaaagaagt acggggaatg gataccgttc
    19681 ctcaagcaat cgctgatgca aaaaaaaatg cggaaagatt aggatatcac aacactcgat
    19741 atgaagtcgg gacagcagag caagttctgc ctaaatggtt gaatgaaggt tttcagcctg
    19801 atggtatcat cgtagatcca ccacgtacgg gtttggataa cgcattgatc caatcattat
    19861 tgaaataccc tccaaaaaaa cttgtttata tctcctgtaa tgtttctaca cttgccagag
    19921 atctggttaa tttaagtaaa aagttcaaga ttgaatattt acagtccgtc gacatgtttc
    19981 ctcagactgc acgttgtgaa gttgtcgtga agatgacgag actcgctgac taaatgaaag
    20041 aggttgtgaa aaagtctgcc ctgtatcaag taataagaac agccaaacaa aaatagctct
    20101 tcatgtttta tgaatactgt acttgtgacc gaagagttaa caggaggaca ctatttattt
    20161 aaaaaaaggg ttgtgacata gtttttgtca cagccctttt tgattattat tggatattca
    20221 cgtctatcac ttgataaaat gcatttgtcg tatcgctgat attccatact gctaaaatca
    20281 cattatatcc ttttcgatcc tgcggaagtg tgatcgtttg gttgactgtt tttggtggaa
    20341 cagttgcttt gtcgtctatt tttgtgatca attcaaaatc actaaatttc aaaggttgat
    20401 taggattcca agttggtttg gtaatgtagt aatcccaagt acttgtccga tgttgtgctg
    20461 ttaaattcca agtaatattt aatgttcctg gattaaccat gtttttatgc catctgttag
    20521 atgtctgttc atctaaaggc tcaaagccgc tgactcctgc actagcaatt tttccatcaa
    20581 tgaatgtatt tttggtagct tcgatacttt gcggttccca ttgtgctctt ccgacatttt
    20641 gatttaagtt tcctgctgct gatgttccta ggtatgcacg gcttcctgga gaagtaatat
    20701 aaccatgcgc aaaacctgga atgctaaaac cgaacaaccc taaactaact aacccaataa
    20761 acaataacgt actaatcttt ttcatgtaga aacctccttc atttgataac cacattgtaa
    20821 acgctttatt gtattaacac aaacattatg cttttataat tttatgttga agtaaaaaag
    20881 gtgaaaaaag taagatgcag attataacag aagtagacga gaaaagtaca aatagaaaac
    20941 atgagaatca aatcatatga tctttttcaa aaacatcatt catataaaca aatgttcaac
    21001 tttttaataa caacaattat caattttttt cttacttcac aaactaaata caggtcttgc
    21061 tgtgagcctt catggtaaac taatattata gaaaggagtt agtaaaatga agatgataaa
    21121 agatattcgt ttttgggttt gtgttatagg aattgtcata ttaggctttt tatcagggtt
    21181 gcttagcgga aatcccggaa aatattacta ttctttgcaa cttccaccat ttgcaccacc
    21241 tagctggata tttgggccga tgtggacctt actgtatata ttgatgggta tttctcttta
    21301 tttgttactt aatcacaaca acaaaaaaca gcgaaacaat cttgtcggtc tttttgtgat
    21361 tcagttcatt tttaatttta tatggtctgc tctatttttt aatttaagaa atattttcat
    21421 tgcagcaatc gatattacac ttttagttat tttcttgtca gtgcttatgt atcagttgtg
    21481 gctgcatcat cgcctgacaa tgtggctcat gattccttat tatttatggg tactattcgc
    21541 aactctattg aattattcaa tttatttttt aaactaagcg ctaaaaagag acgtcctgta
    21601 atttaacaca tagaaaaagt gccacctaag ttagattttt tgtctaattt aggtggcact
    21661 ctttattaac caaactattt ttttctagta cccacttgtt gttccatact cattattctg
    21721 gtcataacca taaccatttt gttcctgtcc agcgtaagga tcataacttt gagtatcttg
    21781 acttggtgta tagtactgac ttgtatcttc tgtttgctga taataggtat tcggatcttg
    21841 gtactgatca tatgtgtttt ctgaatagga agaataatcg gtgtaccctt cactattcga
    21901 tccatcaccg tttgtcgaat cgccatcttc cgaatcatca aagaacatca ttgaagcatt
    21961 ctcatttttc aaattaggta atgtatcgct tgtttttata tttaattgtt tcttcagttc
    22021 attttggatc gaaagaagat cattttttcc taaaatttga tagtagatat cattgatcat
    22081 ttgtccttcg ccttgcaact ggtcttgttt gattgtttca aacgccgggg tgtagttcgt
    22141 cgctacatca agcatgtcat cccaatctaa gtccgtcgtc acattgttct caactgcttt
    22201 taaaattttt ctataattgc ttacgccatc taaacttaaa actttattga cgattttcgt
    22261 cactacttcc cgctgacgac gttgtcgacc gatatcgcct tctggatctt cgtgtcgcat
    22321 tcgagaatag gctaaacctt tttctccatt caatgtttgt tcacctactg gtacatggac
    22381 accatccaaa gtaaagtcaa ttttattatt cactttgatg ccaccgaccg cattgatcaa
    22441 atcttccatg ccatccaaat tgatcgttac ataatgatca ataggaatat cgagcagttg
    22501 ctcaattgaa tccatggcca tttcgacacc accaaatgcg taggcatgat ttaatttatc
    22561 taccgttcca taacctacga tattcgtata aatatctcgg tccaaactga tgatcgttga
    22621 ttttttttgt ttcggattaa ctgttactac catcatcgta tcagaacgtc cttgttccgt
    22681 tcttcctaat ccaccggtat ctaaacctaa tagcaaaacc gaaaaaggtt ctttgtcatc
    22741 aatgcttact ctctcagaac gtttggagga tattcgatta gagtttttag aaattttgtt
    22801 tactgtttgg ctcgcttctc ccataaatct gattccgtat gctgataatc cggcaatcag
    22861 caaaacgaat acccctaaca aggttaatag aactttttgc catcgcttca taatacacct
    22921 ctgtttcaca ttttctaaaa tattgtctca tataactgtt caatcagcaa attcactttt
    22981 taatcataag aaaactttaa aaaacatttt tgtgtaacga ttattatttt ttaaaatttc
    23041 tcaaaattct taactatcat accataccgt gaaaaagatc gaaagaccga atacacgcag
    23101 ttcgcaatcg ttttatcttt ttttatgtat tttttccaga aaaagcgaac aacaaggtat
    23161 tttccaaata atcgtgcagt aaaatgatcg cacatacctt tcccatcttt ttaacagtct
    23221 ttgcctaccg ttcagattat cattcgattt cacgacaatt tcttcctatg actggtcctt
    23281 ttgttaaacc catcttctat tcgttttatt tccagctcac tgcatggctc ttaaaatgac
    23341 tcgaactagt gattgcatta ccatcatctt ttaatggata gatatgctcc tcttcaaagt
    23401 aaaacgattt tcttttaaag taatagaatt gacatggtaa tgagtatgta tactagtggt
    23461 ttcacaaatg gtcttttatt caacatccaa aaactgaaat ttctttaaaa tccattacta
    23521 acgaagaaaa tgttccttgc ttgataatta gttaggacat gtcaagtgaa caaatgcatt
    23581 tacttagcca aaaaatctaa aaacttcata aatagacaaa aagacacgac tctctaagaa
    23641 gtcgtgtctt tattcaacaa attattataa tttagtcact tcagcagctt gaggtccgcg
    23701 tgcaccttca acaatcgtga attctacttc ttgaccttct tctaggctct tgaagccgtc
    23761 tccttgaatt gctgagaaat gaacaaatac gtcatctccg ccgtctactg taataaaccc
    23821 aaatcctttt tcattgttga accatttaac cgttccgtgt tccataaatg tatatcctcc
    23881 aaaaaagttc ttgcaagtaa ccttgcaaga acattatacc caatttagaa aacgctagca
    23941 agctttcgct cttttatttt ccaaattatt tttaagactg atatgacgga ggaaatggtt
    24001 attttgctgc ttcgaaacgt ttgtttactt catcccaatt aataacattc cagaatgctg
    24061 caatataatc tggtcggaca tttttatatt tcaaataata agcatgttcc caaacgtcca
    24121 aacctagaat tggtgtcttt ccttccatca atggagaatc ttgatttgca gtagaggtaa
    24181 tagctaattt tccattttcc attacaagcc aagcccatcc agaaccaaat cgtccagcgg
    24241 ctgctttttt gaattcttct ttaaaagaag aaaaatcacc aaaagcttca ttaatcgctt
    24301 cttttatttc tcctgtaggt tcgccaccag catttggtgc catgatttcc cagaaaaatg
    24361 aatggttagc atgtccgcca ccattgttac gtacagctgt cttgatatct gttggaatag
    24421 cgtccatatc agataataat tcttctattg ttttttcgcc taattctggg tatttctcaa
    24481 ttgctgaatt taaattcgtc acataagtat tgtgatgctt atcatgatgc agatgcatcg
    24541 tttcttcgtc aatataaggt tccaatgcgt catatgcata aggtagatct ggtaatgtgt
    24601 aagtcataaa aaaattcctc ctcaatgttt tcttaataat gtctacatta caaagagtaa
    24661 caaagagaag gaaattcttc aattgttatg cctagtcgtc tttttcactt tgacctactt
    24721 cttcttcaaa aacctcagtt tctttaaaaa taaacaactt gctgaaaata tagttcgcaa
    24781 ggataacaat aatttgagtg atgattttcg ccacatactc attgctgttc ataacattga
    24841 tcaacaatac cattgccccc atgtccaatc cgtaagaaag gatacggtag aagaagaact
    24901 taccgcattc aataagtgca tgtgtaaaat tcggtgattt tgaatggaat acccaaagtt
    24961 tgttcgttac aaaagcaaac aatacagaac aaaaccacga aatagaattg gataaggcca
    25021 tacttaaatc aaaatattgg cgtgcgagag caaaactaac aaaatttaca atcgtagcca
    25081 aaccaccaaa aaagagatag actagtatct cccaatatcc ttttgcttct aaatatcctt
    25141 ttaactgtcg atactgcttc atgagccaac ctaccttatt aaaaattctt atttatcata
    25201 ctctatattt caataacttt catctttatt taaaaaaatt aatttttttt gatcctttgc
    25261 ctttgtctct gcttggatcg tcacatgtgt aataccatat tttaatttta ataaagaaat
    25321 tatacgagga tatatctttt caatctcgct tagtagctga tcttcaaaat caatatgtcc
    25381 gtcgaaaata attatctttt cgctatactg ccaaatatgg acatgatgaa catcctttac
    25441 cccatcgatt gcttcaattt cttgttccat tttttcatag tcgatagcag gtgcactttg
    25501 tatcaaaata gcggcagctt ttttgatcac cattatcgct tctatcaata tatatatcga
    25561 aatgatcagt gtaataactg gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc
    25621 tccgataatc acacttaccg aagacagtgt atcacttaac aaatgcaaat aactagattt
    25681 tatattcaaa ctttcatctg cttcttgtcg taataataat actgagaaca aattcgcgag
    25741 caatccaata actgctactg ttagcatcaa atgactattt atcttttctg gtgaagtgaa
    25801 ccgcttgaat gcttcaacgg ttaaaaacaa ggataatatg atcaatatga atgagttgat
    25861 gaaagcactc aatatttcta aacgctggta gccatacgta cgttttttat ttgcttcttt
    25921 tcgggccatt ttttgtgccc cgtaagaaaa aataattgcc aatgtatcac taaggttatg
    25981 gaaagcatct gaaacaagag acaaactccc tgacataatc cctccaataa attctgataa
    26041 agtgatcacc gcattcaaaa ccactactcc aaatagtttt tttgatgatc tctgtggctg
    26101 tgtaggcact attccttcct ccattttatt tatattggat agtctttaga caagaataac
    26161 atttttttga tatttcacca atcattcagc tgtttcctta aagcacttgt atttcacttg
    26221 atacttcgct agaattgcaa gtgatgacac tactagatga ggagtactta tgacaacaaa
    26281 acaattaatt atcagttcct ttatacgatt caaagaccta aaggaggcga gaaatgtccc
    26341 attttggaaa gccattgtct atcttttagt tttgagtata attatggctt tgccaatctc
    26401 ttttcaagtt tttcaagtct taaacaacat tcaattggat gggcaaaaga ttgctactcg
    26461 cattcctgat tttacaatcc aaaacgggca gcttcaaacc gaagaaaaag aaggattcat
    26521 ttaccaaaca aactccatta tcttcacatt tgatccagag ggaaaacgaa cagaacagga
    26581 catctctact gatttgatgg ggaattttgt tagtgtcggg atgttaaaaa acaaacttat
    26641 catagctctt cctaatacag gcacgacttc tgctttgttg aacaataacc aattagaact
    26701 tccctacacc aatgaatctt taaaaaactt aacaggtaag cagctccgtt cttttttaag
    26761 tgaagcatct ataccacttt ggataaaagt cctgacattt atattttcta tttatcctag
    26821 ttttctgaat ctgctgatca ctttgctatt taccaacatt gctgcttttc tctatgcacg
    26881 ctttcgacta acaaaagcta cttttcttga ttgtttaaaa acattgatct attcaatttc
    26941 attgcctact ataattgcaa caatcttgat ggtctttctt ccttcttttg acactagcgc
    27001 cttcattgct tttgcaggaa tctttatttt tgctcaagct gtcaaaggat gggcaaaaat
    27061 atcgatcaat taagaaataa agacaactcg ttatttctac ttcaatagac ccttttagag
    27121 gttgtgacat ttttttgtca cagccccttt tttatttgaa aaatatttct ttctcaaaaa
    27181 acaagcaagg cgcttcaaag ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg
    27241 gaaattaact atgtcagaaa gaatcattta tgcagatcgt ccatttcgtg atgaatttat
    27301 cgaagaaatc catactatag cgcctggtta tatctttaaa acagaattac aaccagagga
    27361 tctacaaaat gtagagatca gtttgggctg gaactcagac taccaagaaa aactattagc
    27421 ctcttctacc ttgaaatggg tccaatctat ctcagctggt gttgatcagc taccccttga
    27481 gactttttca aagaaacata ttttactttc aaatggtagt ggcatccaca gtgaatcgat
    27541 tacggagcat gtcttaggaa tcatccttgg gtattctcgt ggtctttttc aagcacaaaa
    27601 ggcacagagt gacatgaaat ggttaggttc atcggtccat taccaatcta tccaagggaa
    27661 aaaaatacta atcattggaa ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc
    27721 attagacatg aactgctatg gaatcaatac atccggtcat ccagcagaag gaatggaaaa
    27781 gactttttct ttaaatcaac taaaggaagt tcttcctgaa gtagatttcg tagtcaatat
    27841 cttacctcta acggaggaaa caactgggct ctttgatcag catatgtttg aacactttga
    27901 tccaaaatct gtattcatca atgttggccg tggtgcttct gttaagacac aagatctggt
    27961 gcaggcttta aataaccagc aactttcctt tgcagcatta gacgtttttg aggaagagcc
    28021 tcttccacaa gatcatcctc tttggaaaat ggacaatgta ctgattactt cccacattgc
    28081 aggtttgaca cctgattttc aaaagaaatt aatggctatt tttttaacca atctgaaaag
    28141 ctactttgcc acccatgagc ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata
    28201 gctgaaaaca atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc
    28261 tttttttatt gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca
    28321 agtaaatcat atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca
    28381 tccatttcca ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa
    28441 ccatcatgaa cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct
    28501 ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc
    28561 ccaacaacct actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca
    28621 tcgcctgtct ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca
    28681 ggagaagcga taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt
    28741 tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt
    28801 tctgctaaag aatatttatc tgacaactct aggtaatcta aaatattctt tgacactaaa
    28861 ttatgcgcaa tccttattcc catgtctcgc tcgggatgga cgacgcgatc agctccgatc
    28921 ttttcaagta ctctggcatg atactcattt tgggcttttg ctaaaatatt tggtacgccc
    28981 atttccttta ccatcagagt aactaagata ctggcttgaa tatcttctcc aatagcgaca
    29041 attacatgat cgaaatttct tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt
    29101 gccacaacag catgagtggc tatattcata tattcattga cccgatcttc attactatca
    29161 atagccagta cttcttgacc agcttcgatc agtgttcggc agatacttcc gccaaatcgt
    29221 ccaagaccta caattgcaaa attttgtttc accgttttcc tcctttagta tcttcttttc
    29281 tcagtataac aaagccttta caaaaacaaa agcaagcatc attattgtaa aaaagccaag
    29341 aaaaataata gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac
    29401 caaaccatgt ttgaatgcat agatagcggc ttgtgtacgg tcttccactt ctagcttagc
    29461 caatatattg gaaacatgtg tttttactgt tttcagtgta ataaacaatt cgtccgctat
    29521 ttcctgatta ctttttcctt cagaaatcag cattaaaatt tcattttccc gattcgttag
    29581 ttcttcatgt aatacaggct catttcgccg actcattttt tccatcattt ttgttgtcac
    29641 ttctggttct aatactcgct cgccacgttg cgttgccctt atagcatcag caatttcatg
    29701 tgcggtagag gtcttcaaca aataccctgc tgcacctgct tcaatggcag gataaacttt
    29761 ttcgtcatca ataaaactgg ttacgataat gattttagct tctggccaat cttttaagat
    29821 tgcttttgtt gattcgatac catccatttc ttccatgact aagtccatta aaatgacgtc
    29881 cggtcgaagt gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt ctccaattac
    29941 ttcaatgtct tcttgaatag ataaataaga agagacgcct aagcgcacca tttcatggtc
    30001 atctactaat aaaactttta tcatccttat ctatgcctcc ttcatcaaag ggaccttgat
    30061 ttctacactt gtaccttgac ctttaaaact aatgattttt actgttccac caataccagc
    30121 tactcgttct ttgatattgt tcaatccata acttcccgtt ttcgtttcat tcatattaaa
    30181 ccctgttccg tcgtcaataa tacgtaacaa aagattattg tctattttgt gcaaatatac
    30241 ctctaattca ttggctttag catgtcttaa tgtatttgat aacaattctt gaacgattcg
    30301 gaacaagtga tcctcaatgg aactagatag ttttacatct tctacatccc atttcagcga
    30361 aatctggatt ttattttgca attccttcaa taactgctcg ataccttgct ttaaactttt
    30421 ctcttctaaa ttgactggac gtagatgaag aagcagcgca cgcatctcag actgggaagc
    30481 gttgatgatc tctgctacca ttttcaactg cttgcgaaac atttctggtg tctcgctttt
    30541 ttctgcttgc tccgtcaatg ccgacatcat catcattgct gcaaacaatt gttgcgaaac
    30601 tgaatcatgc agctcacggg ctaatcgatg tctttctaat tctaaaattt cttctttggt
    30661 ttgtccatct acatattggg gacggctcgt tacaatttgt aattcactag atacttcaat
    30721 cattttttct tttatcttag tgatttcctt atcaatatct ttgatgtata aatcgtccga
    30781 tgcacttggg atcggaagaa gcaacaattt ggattcataa ttgccagaag aaagtgcacg
    30841 cagcttttct tcgattttcc cgtattttgt cttttgaata agtgataata atagaaagac
    30901 aattaatcct acacctaagg aaatggctaa caaatggaag ataagcggaa catataataa
    30961 acgtgcacgc agtaattctc cccaccaatg actttgattg ctggcataaa aataagtgaa
    31021 aagtgaaaat agaataataa ggaaagccgc aatccccgaa taaacagcta gcatcgctct
    31081 ggatattttt cccatcatat tcgtatcacc tcgacatctc caatcaaggt attagtaata
    31141 atcttcaatc tacgagggtt ttcatcataa tcactgctgt agattttgat ttgttcattt
    31201 ttcaaagaaa attgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaat
    31261 cggatggcta cgcccaaagg tactaagatc cgtgttctgc ctatcccttt tcttacaatc
    31321 acaatattat catcttttgg caaaagcgta ttgcccaaat ctatgatagt atcaccagaa
    31381 atgacggcga tgttgatatc gtcccattca tagacgtcgt ttccaatacg ttggttaccg
    31441 aataattgct gtcttttccg ctcattatta tgtgttttta attgctctgt ttggatcatc
    31501 atgatctgtt tctttcgcca aaaagcattt ttggttaaat ctatccctga aatctctaca
    31561 cctttcagac caataaataa aacggcaaat acagccatca tccataaagc aggactgttg
    31621 actaagctaa aaaaaatgac caaacttcct aatatcaatt gaaaattttg aaatttagta
    31681 cgaggatgtt tgcgcatagc tagatatata ttgaaaatac cgaatatcac taaaagcaaa
    31741 agtccagtat tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc
    31801 acaaaaaaac gccaagaact attcatcgct ctttctccct cctgacacat tctcatttca
    31861 tcatacctat ttttttaact gttggctata ggactttaga tgtaagatcg tttgttgaaa
    31921 tctaaaatac acaaagagaa aaagatctag agtgtatcat cagtaagatg aactctctag
    31981 atctttcagt cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct
    32041 ggagtagcaa tagttacttc atcattttta cgtttgccaa tcaatgcttg cgcaatagga
    32101 gaatcattgg aaattttgcc agaaaacgga tctgcttcag cgcttccgac aatcgtgtac
    32161 tcttcttctt ctccatctgg tagttcgatg aatgtaactg ttttaccaat tgatacctca
    32221 tctgcatcta caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt
    32281 gtgattcttc cttcaacaaa tgcttgttca tctttcgctg attcatattc agagttttcg
    32341 gaaagatctc cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc gcgtttgact
    32401 gtttttagtt cttctaattc tttttctaat ttttctttac cttctagagt cattggataa
    32461 actttctcta ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt
    32521 ttgaggaata tagtccaaaa aagattacca cgcttttctt caaatgtaaa tgtttttcta
    32581 aaaatgatca atgtctagag cctgtttttc taagaattat ttacatattt ttccactaat
    32641 tccatatgtt cttcataagt tttagcgaaa taaacatttc cagtgctcgt atctgctacg
    32701 aaataataat agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga
    32761 ttgtcaaaag gtccaggtcc atagccggta ttggtataca aattatatgg tgaatctaca
    32821 gctgtatctt catacgtcac taattctttg tgttcaccta atgcgtataa aacagagata
    32881 tctgactgaa gtggcatatt tgctttgatt cggttaaaga agacttgtgc aatgttttta
    32941 cgatcatttt cttttacgcc ttctttttca actagagaag ctagagttaa aacttcttgt
    33001 acagtcaaat ttttctgctt gatagctgaa taatagtttt gcatgaccgt attcgtcttg
    33061 ttcaccattt caatcactag atccttaagc gaagtactcg tgtaataatc atatgtggct
    33121 gggaataagt aaccttccaa acggtatttt gtgttctcag ctttagatgc actttccaac
    33181 agttctggga atttttggtg caactcgtta aagaaagtct catcattcac taaatctaaa
    33241 aattctttct tgtctttacc agttaccttc gctacccgtt cagcaatctg atcgatatcg
    33301 tacccttctg gaatggcaat tttcccatct gcaacttttg ttggctcaga agtaccgcct
    33361 tcttgcaact gtttgccaat ctcgtcaaga gtcatattag gcgctaactg ataatagcca
    33421 gcttgaaagc cagttaaatt tttaaatttt gtataataat tgaaaacaat cccgctttta
    33481 ataatattgt ccttctccaa aatctcacca atctgcttgt tagaagagcc actcgggatc
    33541 tcgacttgga ctaattggtc atctgattta tctaaaggct tcaacccgct atctacataa
    33601 cgatacacgg taaatccaag aaatccgcca ataatcaata atgtaagagc aatcactaaa
    33661 atgatcttcc tcacaatctt atcttctttt ttacgcagat ttctccgctt gtcgattata
    33721 tctgctgtag ctgccttact ggcttctttt ttatctttac ttgggtgttg tttgtttcct
    33781 gacaaaacaa ctcctcctta ctatcaagtt cgtaacttta ttcattatac atgatatttg
    33841 aattggcgtg aatgatttta tttaaat
//
LOCUS       DAHHPA010000021        32206 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.022, whole genome shotgun sequence.
ACCESSION   DAHHPA010000021 DAHHPA010000000
VERSION     DAHHPA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.022"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(143..523)
                     /gene="yajC"
                     /locus_tag="NBH55_000904"
     CDS             complement(143..523)
                     /gene="yajC"
                     /locus_tag="NBH55_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCD1508908.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(620..1765)
                     /gene="tgt"
                     /locus_tag="NBH55_000905"
     CDS             complement(620..1765)
                     /gene="tgt"
                     /locus_tag="NBH55_000905"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCD1508909.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1983..2681
                     /locus_tag="NBH55_000906"
     CDS             1983..2681
                     /locus_tag="NBH55_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCD1508910.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2849..5488)
                     /locus_tag="NBH55_000907"
     CDS             complement(2849..5488)
                     /locus_tag="NBH55_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCD1508911.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6107..7138)
                     /gene="queA"
                     /locus_tag="NBH55_000908"
     CDS             complement(6107..7138)
                     /gene="queA"
                     /locus_tag="NBH55_000908"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCD1508912.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7331..8506
                     /locus_tag="NBH55_000909"
     CDS             7331..8506
                     /locus_tag="NBH55_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCD1508913.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8510..9151
                     /locus_tag="NBH55_000910"
     CDS             8510..9151
                     /locus_tag="NBH55_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508914.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9148..10065
                     /locus_tag="NBH55_000911"
     CDS             9148..10065
                     /locus_tag="NBH55_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1508915.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10062..10733
                     /locus_tag="NBH55_000912"
     CDS             10062..10733
                     /locus_tag="NBH55_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508916.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11274..11762)
                     /locus_tag="NBH55_000913"
     CDS             complement(11274..11762)
                     /locus_tag="NBH55_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCD1508917.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11963..12229)
                     /locus_tag="NBH55_000914"
     CDS             complement(11963..12229)
                     /locus_tag="NBH55_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD1508918.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12229..12492)
                     /locus_tag="NBH55_000915"
     CDS             complement(12229..12492)
                     /locus_tag="NBH55_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD1508919.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12968..13153
                     /locus_tag="NBH55_000916"
     CDS             12968..13153
                     /locus_tag="NBH55_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1508920.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13295..15118)
                     /locus_tag="NBH55_000917"
     CDS             complement(13295..15118)
                     /locus_tag="NBH55_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD1508921.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15716..18421)
                     /locus_tag="NBH55_000918"
     CDS             complement(15716..18421)
                     /locus_tag="NBH55_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCD1508922.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18727..19674)
                     /locus_tag="NBH55_000919"
     CDS             complement(18727..19674)
                     /locus_tag="NBH55_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCD1508923.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19864..20133
                     /gene="rpsN"
                     /locus_tag="NBH55_000920"
     CDS             19864..20133
                     /gene="rpsN"
                     /locus_tag="NBH55_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCD1508924.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20309..20671)
                     /locus_tag="NBH55_000921"
     CDS             complement(20309..20671)
                     /locus_tag="NBH55_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD1508925.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20682..21803)
                     /locus_tag="NBH55_000922"
     CDS             complement(20682..21803)
                     /locus_tag="NBH55_000922"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCD1508926.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21819..22169)
                     /locus_tag="NBH55_000923"
     CDS             complement(21819..22169)
                     /locus_tag="NBH55_000923"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCD1508927.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22299..23810)
                     /locus_tag="NBH55_000924"
     CDS             complement(22299..23810)
                     /locus_tag="NBH55_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1508928.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24132..25484)
                     /locus_tag="NBH55_000925"
     CDS             complement(24132..25484)
                     /locus_tag="NBH55_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCD1508929.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25637..26713)
                     /locus_tag="NBH55_000926"
     CDS             complement(25637..26713)
                     /locus_tag="NBH55_000926"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCD1508930.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26909..28654
                     /locus_tag="NBH55_000927"
     CDS             26909..28654
                     /locus_tag="NBH55_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCD1508931.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28934..29371)
                     /locus_tag="NBH55_000928"
     CDS             complement(28934..29371)
                     /locus_tag="NBH55_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCD1508932.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29355..30728)
                     /locus_tag="NBH55_000929"
     CDS             complement(29355..30728)
                     /locus_tag="NBH55_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508933.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31050..31538)
                     /locus_tag="NBH55_000930"
     CDS             complement(31050..31538)
                     /locus_tag="NBH55_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1508934.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31560..32162)
                     /locus_tag="NBH55_000931"
     CDS             complement(31560..32162)
                     /locus_tag="NBH55_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD1508935.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9207 a         6884 c         5942 g        10173 t
ORIGIN      
        1 gttatttatt attatttata tgacacaccc gattattcaa tattgagcaa tatatcccat
       61 gctcttatct ctttcttttt tgaataaaaa atgaggtagg acatctttct gccctacctc
      121 gtctttaaat aagctttgat acttattttt cttccttctc ttctgtctct tccagcgttt
      181 ctgttgtttc agtttttact tcaactgtct cagctggttc ttctgttgtt tcaacagtag
      241 tcgttgcatc atttgttacg acagtccctg gtttgaccgt tttgattgct gcacgatcgt
      301 actcaaggaa gatcccttcg cagtctaaaa caactgtccg tttgtcattg tcgatttctg
      361 aaacaactcc atgtaatccg ccgatcgtta cgactgaatc acctggtttc atgctatcta
      421 acaagttttg acgttcttgc tgttgtttct tttgcgaacg tgacataaaa aaccacattg
      481 ctaatagcgc gataaacatc acaagcattg gtaatgttcc catttttttc acctcactaa
      541 ttctgtatct acaaaaaaca ctttagcaga aaaatgctga taacactagt tttttactta
      601 atttttaaaa gattttcctt tagaagcttt ttgcgttttc tttattgaac ccgtattctt
      661 caaaaaaggc ttctctaaat tcaagcaaat tatcatccat gattgcttga cgtacttgtt
      721 tcattagatt caacaagaag tataaattgt ggtaagaagt caaacgaatc ccaaatgttt
      781 catcacattt gatcaaatgg cggatatagg cacgtgtata gtttttacat gtataacaat
      841 cacatttctc atcaattgga cggaaatcat gggcatattt cgcatttttc actactagac
      901 ggccttgaga agtcatacat gtgccatttc gagcgattcg agtaggcaag acacagtcaa
      961 acatgtctac tccacggata acaccatcga tcaatgaatc agcagcaccg acacccatca
     1021 agtaacgtgg tttgttttcg gggatcaacg gtgtagtgaa ttctaataca cgattcattt
     1081 ctgatttagg ttccccgaca gataacccgc cgattgagta accagggaaa tccatcccga
     1141 ctagatcttt cgcgctttga cgacgaagat cttcaaaccc tgctccttgg atgattccaa
     1201 ataatccttg acggtctgga ttagcatgag ctttcaatcc tcgttctgcc catcgagacg
     1261 tacgttctac tgattttttc acgtagtcgt aactttcatc gaatggtgga cattcgtcaa
     1321 aactcatcat gatatcagag cccaatttat tttggatgtt gattgctttt tctggtgata
     1381 aaaacatacg cgaaccgttc aagtgatttt tgaaatgcac gccttcttct tcgatattcc
     1441 gcatatcact taatgaaaaa acttgaaagc cgcctgaatc tgttaaaatc ggttgatccc
     1501 agttcataaa tttatgcagt ccgccagctt cttctactaa gtcttcacca gggcgtagcc
     1561 ataaatgata tgtattactt aaaataacgc cagcacccat ttctttcaac tcttctgggg
     1621 acattgtttt tactgtagct aatgttccta ctggcatgaa cattggtgtg gggaaagttc
     1681 cgtgtggcgt gatcaattcc cctaaacgcg cgccagtatg tttttctttc ttgattaaac
     1741 gatagcgaat ggcaggttgc gtcattcatt tccctacttt ctatataaaa tatcttgaca
     1801 actcccacca tcataatcgt ttcaagtgct ttttgcaagt tccctcggct tccttctact
     1861 ttatttaata ttcttttttt tatccttttt ttatgaataa caaaaaaaag aaaaacatta
     1921 tgacagaaat attttttgat acatggtata ttggtgttat taaagaaaag gagggaaaaa
     1981 tcatgcaatc ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa ggacgttggg
     2041 gacaagctgt tttactaaat ctaatcccta cattgattgg tattgctact attcttctta
     2101 ttatgattcc tattatcttg tttgccacca ccatatatga tggatcagcg agcgtctcag
     2161 aagcgacaac ttcaggaggc ggtagttcag gaggcggtgg cggaattatt tccagcatca
     2221 ttagcgcttt gttcatgagc ggtatttctt ggacttatct tgatatttta cgtggacaaa
     2281 aagatcagat cgaaccattc aaagatgcat ttcgtggatt caaaggtttg tttatcggcg
     2341 gagtgatcag cttagctgtg ctgatctcga tctttacaac attatggggt ttgctattag
     2401 ttattcctgg aatcattaaa agctattctt attctcaaag ctattttatt tactacgata
     2461 tcgtccaaga aacaggacaa acacctaaag tactggatac gatcactgca agtcgaaaat
     2521 tgatggatgg ttataaagga caacttttct ggttagatct ttcatttatc ggctggcata
     2581 tcttagcatt agcaactgct ggtattggtt atctatggct cagcccatac atttctgcaa
     2641 caaaagcagc attttatgaa caattgccaa aagatattta aaagagagtg tgacttgtga
     2701 ccgagggggt ggcaggagaa caccgtttat cgagaggcag taaaagaaac attcagccca
     2761 ttagcaaaaa ggaggtgtga caacattcat gtcacacctc ctttttgtat agttccagta
     2821 aaaagaaaat caacttagaa acagttaatc attcctcttc ttctgcttcc ggtaatcgat
     2881 ggaacttgat cagttcattc accgcgattg gtacgataga taaaatccct accaagaacc
     2941 agtgattcaa actgatgtgt actaggccga atagttcttg cgtaaatggc agcaaagcaa
     3001 cagccgttgt aatagctaat gccagtaacg ccatttctac caaagcttta ttggtcgcta
     3061 aattgacttt gaagatcgat tggctgctgc gtacattgaa cacatgcaga atcgatgaat
     3121 aagctaagat caagaatgca acagtttgac cgacatgctg acttgcgtct acccatgggc
     3181 tgatagtatc gacataacta cctaaataat aaccaaacaa ggttacgatg gtgaagaccc
     3241 ctgcatttat ggcaattttt tgcaataaac ctcgagcaaa gatcccttca tttttcggaa
     3301 tgggtggctg atccatgatg ccgtgttccg ctggctctct tcctaaagca aatcctggaa
     3361 gtccatcagc aactacattg acaaacagta gttgcacagc tgtaaatggt gctccccatc
     3421 ctaacagcat cgcaatcaaa acaataaaga tctccgaaat attacagctt agcaaaaagt
     3481 tgatagcttt acgaatattc tgataaaccg aacgtccttg actgacagcg tctacgatcg
     3541 tcgcaaagtt gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca tctgttcctg
     3601 tgatccccat ggcacaccca acgttacttg ctttcaacgc tggtgcatca ttcacaccat
     3661 ccccagtcat cgctacgact gcacccgtac gttgccaaga tttgacgatc cggatcttgt
     3721 cttctggcgt tacccgagca tagacagaga gagctttgac acgcgcatct aattcttcgt
     3781 ctgataattg atgcagttct gatcctgaaa gtgcttctga cggatcattc aagataccta
     3841 attctttcgc tatggcactg gcagtcacca catgatctcc agtgatcatg actgttttga
     3901 tgccagcctt tttcgctcgc ttgattgctc ctttactttc tggacgtggt ggatcgatca
     3961 ttccgatcag accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa atatcttgcg
     4021 gttcttcgtc ataagtagca taaccaacag caatcactct cagtgcccgt ttaccaaagc
     4081 gatcattaac gatcgccgct tgttctacat cgcctgaact aaagcgcgga gcaagaacat
     4141 cgaatgctcc tttggtgatt gagatgtatt tttcgcccat ttggtgaact gttgtcatca
     4201 tttttcgttc agaatcaaaa ggaagctcgt ttactcgtgg atatttttct tctaattctg
     4261 cctttgtatg ataattctct tcaacagccc ttacgatggc tacttctgtt ggattacctt
     4321 tgataacaag gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt gcagccattt
     4381 tcaatatttc catcgcgctg ttcgtcattg aatcttcgat attcgttact tcatcttctt
     4441 tgctccatac ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt ttgtctgagc
     4501 agataacatt agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg atcgcatgtt
     4561 tcctcgccat cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca gtcaaggttt
     4621 ccggtacagc tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg agtggttctc
     4681 cttgcagttc accaatcaca aatactaaag ccgcagccgc taaagcgatc atactgattc
     4741 gtttgcctag ctgattcaat cggacctgta aaggtgtttt ttgcatagta ttttcgttca
     4801 aaagatccgc gatcttaccc atttctgtag ccatgccaat ggcagtgacg atcgctttcc
     4861 cacgacctgc agcaaccgta catcctttaa ataccatatt aagttggtca cctaatgact
     4921 catcttcttc agcaataaat gctgcatctt tttcaactgc ttcactttct ccagtcaacg
     4981 ccgattcttc tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca gaaatagcat
     5041 caccggactc taaaacaaga atatcaccca ctaccagttg ctctgactca acagtggtca
     5101 ctttgccgtc tcgtatgaca gttgccgttt gtttattcat atcttgcaag gattccagtg
     5161 ctttttctgc gtttccttct tggacaatcg ccaataccga gttgatcaca acgatagcga
     5221 tgatcagcag tccttcaaat aaatccccat gttctgtagc aaatgcggta taaaaggaaa
     5281 tcaccgctgc tacaagaagg atgatcgttg taaaatcgga caagcttcga aagaattttt
     5341 tgatcatcga ttctttcggc gcctcatcga atttattcaa tccctgcttt tttcgccgtt
     5401 catccacttc ttgtgaagac aacccagtat ctggatctgc ttctgttacc ttctttactt
     5461 cttctattgt ctgcttgtat tcttccatcc tattcctcct ttaaaatctt cgtacaaaaa
     5521 aaggtactta ttttactata acatcctttt tttatttact tcaaataaag ggcttatcga
     5581 ttatttgtta agaaagcgta aaatttaaag tgcttatatt tgcaaacgct accaaaggat
     5641 gctattatat agatgtaagg aaggaggata ccatcaaata gacagaagaa tttctcgata
     5701 gaacagtgta agcaaacaaa gttgttagaa gtacaggttt cacccagaca tgcgtgtaga
     5761 gtacaaacct ataaatagtg aatgcgtatg gatgtttgga tcacgaagta tccgaaacaa
     5821 gaagtaatgc gatacgtaac aattgaataa cgtaaacgtc tgaaaattga ataggaacaa
     5881 gagatgtttc aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac atgaagaaat
     5941 tcaacaccgt gctttgcatg tatgatggaa agttacaatc atttctgttt atttgaatcc
     6001 ttccgaggta caagaaagcc gagcttatcg agagctcggc tttttctttg tcataaaatc
     6061 tttgtaaagc ttttttgtta ttcaaaacta gcggcttttc ataaccttat ttcacgaaca
     6121 tcgcatctcc aaaactaaag aaacgatagc gctcttcaat cgcatgctgg taagcagata
     6181 aagtcaattc tcttcctgca aaggcactaa ctaacatgac taaagtcgat tttggcaaat
     6241 ggaaattagt tgagaaagct tctaccactt tgaattcata gccaggcgta atgaaaatat
     6301 cagtccatcc gctgtctgct ttgatttctc cattaaattt cgttccaatc gtttctaatg
     6361 ttcgaattga agttgttccg acagccacga ttttcccgcc atttttacgt acttggttca
     6421 atcgctcagc cgcttcttcc gtcaaacggt agaattcact atgcatatgg tgttcttcaa
     6481 ttttatctac gctgacaggt cggaatgtac ctaaccctac atgtagtgtc agatagacta
     6541 attcaacacc ctttgcttcg atctcatcaa gtaattcttt cgtaaaatgc aatccagctg
     6601 ttggcgctgc agcagaacca ttttctttcg catagacagt ttgatagcga tctgggtctt
     6661 ccagacgttc tttgatatat ggaggcaatg gcatttctcc caatgattca agaatctcta
     6721 gaaaaattcc ttcatactca aagtcaatga tccgtccgcc gtgttccaat tcttctttca
     6781 ctactgcttg caatctgcca tcaccaaaag agattcttgt cccaactttg gcacgtttcg
     6841 caggtttgat caatgtctcc cacgtatctc cttgtgtatt cgttagaaga aggacttcta
     6901 aatgcgcacc agtatctggt ttttctccat ataaacgagc aggcagaact cgtgtattat
     6961 tcataactag cgcatcacca ggctgtaact cttcaatgat ttcatggaaa tgtttatctt
     7021 ccatctctcc agttttccga tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg
     7081 gtgtttgtgc gattaattct tccggcaagt caaaatcaaa atcttctgtt gttaacatat
     7141 cgtcactcac tttctatcat gtgcttcatc attctatcat gtttttcgtt ctgttggcaa
     7201 aatcagcact ttttcacttc tattttttgt tttcctaaat aaaaaaacta ccctatttgt
     7261 attttttcat tgttttaggc atactagtaa tagatagttc ttcattgaaa atagaaaaag
     7321 aggtggcacc atgattgaat ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc
     7381 tgtagaggac gtcaatcttt catttgaaaa gggtgagttc atttgtttta tcggtacgag
     7441 cggaagcgga aaaacgacga cgatgcggat gatcaaccga atgactgatc caacacaagg
     7501 gaaaattttg attgatggaa aatccatcca agaaattgat ccagtggaat tgcgacgtca
     7561 gatcggctat gttatccaaa gtattggatt gatgcctcac atgacgatcc gtgaaaatat
     7621 cactcttgtc caaaaattgt tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa
     7681 aatgattgat cttgtggaat tgccccgtga gatgttagat cgctacccac atgaattatc
     7741 tggtggtcag cagcagcgta ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat
     7801 cttgatggac gagccattcg gtgcgcttga tccaatcact cgtgattcct tgcaagacct
     7861 tgtcaaagat ttacaagagc gtttgggaaa aacgatcgta tttgtaaccc atgacatgga
     7921 tgaagcgatt aaactagcaa gccgtatcgc catcatgagt gaaggccgtg tgatccaatt
     7981 tgatacacca gaaaatattt tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg
     8041 agaagatcgc ctactacaag ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa
     8101 tacagcaatc acgattacac cggaaaaatc attacaagaa gcaatcaaac tgatgaggga
     8161 aaaacgtgta gatacgttgt tagttaccga taattcgaat gtattaaaag gatttatcga
     8221 tgtagaaacg ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca ttttgaataa
     8281 acatgttttt tacgtaaaag aatccgccta tcttcgtgat accttgcaac gcattttaaa
     8341 acgcgggctg aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag gtatcctgac
     8401 tcgtgcatca ttagtggata tcgtctacga tgtgatttgg ggagaagatc ccgcaactac
     8461 tgatgttgca gagtcagcta ctcctgaaga atctaaggag gtctgaccta tgcagcagtt
     8521 ttttcatcag cacggttcag aaattctctc aaaaggtttt gaacatcttt atatttcagg
     8581 acttgctttg atcattggta tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt
     8641 tccaaaagct gccacgattg tgattggctt aaccagtgcc ttgcagacaa tcccttctct
     8701 tgctttattg gcattgatga tcccgctatt tggcgtaggt aaattacctg cgattatcgc
     8761 actctttatt tattcattat tgccgatttt acgtaatacg tacattggga tgaaaaatgt
     8821 cgattggaat tatcgggatg tggcaaaagg catggggatg actgagttcc aatccatttt
     8881 ttccgttgaa ttgcccattg ctatgccaac gatcatggct ggtattcgtt tagctgccgt
     8941 ttacgtgatt gcatgggcta cacttgcctc ttatatcggc gctggtggtt taggcgattt
     9001 gatttttagt ggactgaata attaccaacc agatctcatt ttggcaggta cgatccctgt
     9061 aacaatttta gcactattgg ctgattggct tctaggtctc ttggaacacc ggctgacacc
     9121 gatcgcactt agagaggaga atgaagaatg aaacgtatac taaaaatgac catggttctt
     9181 ttctctcttc ttatacttgc gagttgttcc ttccctggtc ttgccagcaa tacagatgaa
     9241 gacaccattt cgatcacagg tggaatcact tcagaagcac agatcttagc aagtctagtt
     9301 gccggaatgg ttgaacatta tacagataaa aataccgcaa tcatcaacaa tctggctact
     9361 acaactatca accatcaagc gatgatgaac ggagatgctt caatttcagc ggctcgctac
     9421 acaggaacag atttaacgac tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg
     9481 tttgctaccg tgaaagatga atttgagaaa agatacggtc aaacgtggtt cccgtcctat
     9541 agttttgaaa atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta
     9601 tctaaagtca gcgatctgaa aaatgtagcg gatgaattag tagctggtgt agatacttct
     9661 tggatcaatc gtaaaggaga cggttatgac ggcttccaag aaacttatgg cttctccttc
     9721 aattcgatat tacctatgca gattggactt gtttatgacg cagtcgaagc aggaaaaatg
     9781 gatattgttc ttggctactc tacggatgga cggatcgcca gttatgatct agtcatgttg
     9841 gaagatgatc gtcaattctt cccaccatat gatgctgctc cagttgtaga caacaagata
     9901 ttagctgata cgcctcattt aaaagaagcc ttaaataagt taggtcatac catcagtacg
     9961 gaaaaaatgc aggaattgaa ctacgaagca gataataatc tagtggaacc atcagtagta
    10021 gcacaacgtt tcttacaaga aaaccactat tttgaaggga agtgaagcaa ttggataaaa
    10081 tgaatgtctt tcaacaattt ttctattatt tccaggaaaa tggctcctat atctttgcac
    10141 aatttatccg tcacttcctg atttctatct atggtgtcct ctttgcagca gttgtcggga
    10201 ttccggttgg gatcatgatt tctaggagac gtaaattagc aaactgggtg attcgtttgg
    10261 cgaatatcat ccagacgatc ccttctctag ccatgatctc cattttaatc attggattag
    10321 gcctgggagt caatgtcgtg attgttactg tatttttgta ttccctctta ccgatcatca
    10381 agaatacgta taccgggatg atccaagtag acaaaaacat ccttgatgtc ggcaaaggca
    10441 tggggatgac tgctagacaa cgactattca tggtagagtt accactctct gtttctgtta
    10501 tcatggcagg cataagaaac gcgttagttg ttgccatcgg gataaccgcg atcggtgcat
    10561 tcgtcggtgc tggtggtctt ggagatatca ttatccgagg aaccaatgca acagagggca
    10621 catcgattat tttagcaggt gcattgccaa ctgcattaat ggcgatcatc acggactggc
    10681 ttttaggtat tttggaaaga cggctagatc cagccagccg tacatcacgc tgatgttatt
    10741 gtaaaaaaca aaagcttctg cgaccatttg ttggtcgcaa aggctttttt agttctgtca
    10801 tttttaaggc aataatccac tctgattctt ccctccttat gggcaaagag ttgcttttct
    10861 tagctattta tccattccgt atactccaac ttcatactgc caacgggttc atatctgcat
    10921 ccaccgacta agtttttttc ttatccgcta aggcattttg ccaatccatt ttcccatcac
    10981 tcgtcatgga attcatacca catgatctct aaatactcgt ctgcttgttc tttgaattgg
    11041 gcatcaattc ttacatcctt tggcgtggct ttcatttaag ccttttccat cagtcctcga
    11101 ttgttcgatg ccttcagctg catcatcaaa actggtgact gccgtatcgg taacttcgat
    11161 catcttttat acaatcggga acttgccatc ctcatccttg gctaaaaaca tatctagcca
    11221 ttccagcttc tcgccatggg aggtttgcgg ttcctaaaac cggtacacga ttcttatgga
    11281 ctgcatccgt cgcaactaga cttggcgata cgatgatcct tcaccagaag agcacccccg
    11341 taaaccaatt gatcgatata ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc
    11401 cctcgtgatg cattgccgtt gatactgcta ttcatggccg aaagcgccac gactttaact
    11461 tcttccgttt gagtgacatt catgttcggc aatgtcgctt tggatacccg ctctgctaaa
    11521 ggaacgacac tggtattgta tttcgtatct gtatcttttc caaaagacca tgccaacgaa
    11581 tcctctggga accaataaga cgactccggt tgattgtcca ttgctttttt caacatactt
    11641 ttggctgttt ctcgataaac gacatttcca attgtcttcg cctcactttg gcgataatcg
    11701 atggtcgttg ccactgccaa cactaacaac gatgctccaa ttttactgat tttccttgtc
    11761 acacttcttc ctccttgttt tacataaacc cgtcaacatt ccattcctaa actcaaacaa
    11821 tgacccttca aaggtggaga tataggaaca aattaaagaa atcatgcaat aaatccaaga
    11881 aggttggttt tagaacaaaa aaagagtctc ctttttgggg agactctttg ttaactatct
    11941 aaaaaggttg gctcttctca cttcactcat aatgtgtcca ggcagaataa atttctacaa
    12001 ctttattttc ctcatcaact ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt
    12061 atctgccttc atgtgtaggt ctcagctttt caaaagactg cgtgggcata tatggatctt
    12121 ccttcaatgt ctgaatcact tcttcaaatt gtttctttaa attcgtttgc ttgatttttc
    12181 ttaaatccac tttcgcagag tttttgattg cgactgtata gttactcatc aaagagcttc
    12241 ccaatcaata tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt
    12301 atctaaagta cctgtttgct caagaaaaag ggtttcttga atcgctctcc agtcttccag
    12361 cccaatcaat acagcattgt ttccactacg atcactaata atctcgattt cagtatgatt
    12421 ttcatttacc tctttcaata actgataaaa atttttacga gcagtgctag gattcatcgt
    12481 tgtattagcc attgtctctc ctcctttttc tgatataatt atagcgtact tgattaagta
    12541 cgtcaaaaaa atacaataaa gcacttaaat tcagcaaact tttcctcttt tttacataga
    12601 gaaaaagacc cctttttagg gaggatcttt gtccacgctc acttcttact cctttacgac
    12661 tagcggtagc caaacgtaaa gtaattggta acaacgtatc atctttagca aaggtcgaat
    12721 ccaaagcctg tgagtctatg caacaacacc aatacacgaa ggttcctcgt tgcagtgtga
    12781 cttgattgat atcttgaata acctgaggat acgcacgaaa gatttgtaac ggttattcaa
    12841 aactgatttt ggccgtcaag tcttctactg cctcaaaaat gattgcctct ttcgtctcct
    12901 gtcattcatg ttcactatcg atttgatact tgcttagacc gttgggtttg tgaatcacca
    12961 attaaaaatg gcactgagtt atggtaacaa aaaatggtca cttgacgagg ctgagattcc
    13021 tacttatgaa gacacatggc acttaagagt cgaggtaaag gaagaagctg aaaatatagg
    13081 aggctgtaca ttatttggca aaggattcgg aaattatgat caagacatca aaatcaaaat
    13141 gttttacagc taaataggaa gaataataac atttcatacg ctgttcccat ctttaatggc
    13201 ttatagaggg cacctttagc tgagaataag acaaaacaga ccctctagct tcaattaaga
    13261 gctagagggt ctgtttttga ttttaaaact tggtttattt cttcaaatgg tagctatagg
    13321 tgctgacaac gatattttca cgccaagaca atcgcaatct taaccgcaaa cttgtttggt
    13381 tatggagaat attctgccag cgtctgtttg gtacaaattg aggaatcaac acagtagttg
    13441 tatagttttg tttcgctgca tttttactta cgatatctac ataacggata attggatttt
    13501 caatcgagcg ataagaagag tggacgattg aaaaacggac atctgggaaa tgctttttaa
    13561 attctgcttc gatttctttt tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa
    13621 cgacataatc accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat
    13681 tcccgactaa tacaagaacg gtattcccgt cataatcatg tagttttgca tcattttcta
    13741 aacgaagctg ttctgctact tttttataat gatcatggat cttataaaaa atgaacataa
    13801 cgattggcat aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg
    13861 caatgattgc atatgagatg aacgcacccg taatattggc aatcgatttg cttaaccatc
    13921 tttttccttc ttttttccat ttgacgacca tccctgtttg agataaagca aatggaatga
    13981 acacgccgat agagtataac ggaatcaagc gttctgttga gccatggaaa ataaacagca
    14041 atacgatcga acctagcgct agtgtgataa tcccattgga atagcctagt cgatccccac
    14101 gatcttgata catgtgaggc atgaatttat cttttgccag attgtatgca agaactggaa
    14161 aagcagaaaa accggtgtta gcagcaactg ctaaaatcag cgctgtagca aattgaagga
    14221 cgtaatacaa aataccatgt ccaaagacag ctttaccaat ttgagacaaa acagtgactt
    14281 ctttttcagg aacgatccca taccaatagt tgataaatgt gatccccacg aagaagaaac
    14341 ctaagatcaa tgccatcatc gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa
    14401 agaatggtac agcattacta atcgcctcca ccccagttaa agaggaagaa ccagaagaaa
    14461 atgctcgtaa aatcaaagcg atagaaacac ccggcacagc cgctcctgga agtgcagtag
    14521 cttgcaatgg cacagcacct gtcacgatat tatataaacc aacaacgatc agtaacgaga
    14581 tcacaaggat aaatgtgtac accggaaata ataggaacga tgcagattcc ctcagcccac
    14641 gaagattcag catcatcagt agcaaaacga ttgtcacaga aatagcgact tgatgaccgt
    14701 agagtgctgg caccgctgat gtgatcgctt cagcacctgc tgagacagat actgctactg
    14761 tcaacatgta gtcgatcaat aaagagccac cggaaatcaa gccagcattc tttccaagat
    14821 tctcactgct gacgacatat gctcccccgc catgaggata agcatggatg atttgacgat
    14881 atgatagcgt taatgaaatc aataaaataa taacaaaagc ggcaatgggt agcgaatacc
    14941 agatggctgc agcagacaaa gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa
    15001 tagatgataa cgcatcagac gacaataatg ccaaagcagc aaatcgggtt aatttgtgct
    15061 catcattttc agcagatttt aaaggcttac ctactaacaa cctttttaag taatccaatt
    15121 tctaaactcc tttacacaca attttacttc aagttttata gtcgaaaatt tatagtaaac
    15181 aagatgttat agtacgctca ctgaccttaa aagtcaataa actttaagaa aataattgga
    15241 aaaaaacaga aaaatatcaa gcgctttttc aaacgcttta atacagcaaa aatctaacat
    15301 ttttttatgg gctttttctt tgaattctat aataaaagga tagaacttga cttagaaaat
    15361 caagttctat cctttttgat atcttaactt ttggaagaga gatgtaaggc tgctttctac
    15421 ccatcattct tactactcga agcatagcgc agttgtcaca acctcttgat aaacggtgtt
    15481 taaaagcttg catctgcggt aataagccca accaaattaa aaacataaga agctgttttt
    15541 atttggtcgt tcttattact tgatgcagag cagctttttt acaacctctt ggtacacggt
    15601 gttcaatcag ctgaccctcg gtattagctc aaaaaaacgg caaaatatga ggaactattt
    15661 ttccattttt ttctctaata ctcaggtcaa aacactgatt tcacaacctc tttttttaat
    15721 gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt cattttttct tcgatgaacg
    15781 gatagattgc cgttcctacg attccaaaga caacaaacaa accgatcatg atccagattg
    15841 ctttcgtagc atttggccaa taaattcctc ctgcagattc ccgtaacaag tttaccgcat
    15901 gagtaaatgg caagaacgga ttgatcattt ggaagaattt ccctgagact tggatcggat
    15961 agttcccgcc tccgccagag atcgacagta ctaagatgat aatagcgatc cctttaccga
    16021 cattatcgaa taatgcagcc aagacataga cgatcatcat gaacgctaag gcgatcaaga
    16081 cagcaaatga cacgctgtag accggatttt ggacatctac tcctagtaag aaaatattcc
    16141 ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa
    16201 cgaattgttc tcgtttcgta aaattgtttc ggtctttctt tcctaaatag tattctgtcg
    16261 tagcaacact tgaaagaaga acagcgccga cccacagaca tagtgcagta taaaacgggg
    16321 tgctagctga accgttattt gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt
    16381 gtgtaaagaa gtcactttct tttgttgcgt ctagtttcaa taatttgatg acttcgccta
    16441 gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc tttttggata ccagctttga
    16501 tatttggcca gtcattgttg atcaagtccg cagcagcgtt cacagctgtt tcgacttctg
    16561 gcattttttc atttactgtt gtcaatgtac cggtgatttc attttttagt gctggccaat
    16621 cattttggat aaaatcagcg gccaatccta atttttgctg aatgatcggt aattcattgt
    16681 tgtataggtc tgccccttca ttgatgccat tgacgatggt atccatattt ccattcagca
    16741 ttgtattggc atcatggatt tcttgtccaa ttgcaggcat ttctttttga tatttttcta
    16801 aaattgccac tgcattcgtt acagttgctt tcgttgagct gagaagcgaa tcaaagtcga
    16861 tctggtccgc tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg acttggtcta
    16921 gtagagaagt gacagtggaa ctgatttgac ctgtattgat accagcaatg atgccattga
    16981 tattattaac aacggcatca atttgatcta gcaattcatc aatagccttc gtgtcttcat
    17041 tttgaacagc tgtgttcaaa gcagataggc ggttttgcag attgcttagc aagggtttta
    17101 attgatttag ctgatcaata attggctgta atttcgtatt tccagcactt gtttgcaatt
    17161 tagtcaggaa ctcgaccagt ttatcgattg cattttgttg ttgagatatt ccgtcaataa
    17221 agccgttcaa tacattgctg atgtttttct tttcatcatc agtcagctta ccatcttcaa
    17281 tagctgttct tacagtatct attaacgaac ctgtcgttgt tgccacttgt ccaattgact
    17341 tcaatgtaac atctacactg tttgtgatgc ttggtatcgc atctttcagc tgagaggcac
    17401 ctgattttgt ttggtttgct agatcacttg cctgattttc taaatttttc acatctggca
    17461 agatatcttg tacttgttgg atgatcgtca agccttgttt tgcttcatcg attccttggt
    17521 tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat
    17581 tttggatctc tgggattttt tcttgcaaat caaggatgat ttttgcttga tctttcaact
    17641 caggcatttt gtcattcaat gcaacgactt tttcacccat ctcatctact ttagggatgt
    17701 aatccacgaa ttcatttgct ttattcagtt tttccttgat ttcaggtagt tgactttgta
    17761 attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa attttcatct gttgataaaa
    17821 tcatatcttt aactttattg atacttacta ggttcgtatc gatgtcgtaa cctatctcat
    17881 tgaatacttt caataaagtc gaactggctg ttttgataaa ttcatttgtg atttgcgatt
    17941 ggatcgaact tgctccttta tcggtgattt tcggtgcaat cgcattgatt ttttcattta
    18001 ccgtatattc gatttttggt ttcttgatat caccactagt aaaactcagc agatcttctg
    18061 agaaatcctt tggcaagtaa atacctgcgt aatactttcc ggaacggact ccgtcttcta
    18121 attgttcttt tgaatcaacg aattgccatc ctaattgttt atttttatga agcgattcga
    18181 tgacttgctc cccaatggcc acttctttac cttgaaactc tgctggcttg tctgcactgt
    18241 aaacagcaat tggcagctct ccagtatttc catatggatc ccataatgct ttgatgttaa
    18301 accacgcata tagcgaaggg atgatcatga tcgcgatgat cagaagtgtt gcaatcgggt
    18361 ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag cgtatttttg attgactgca
    18421 tagttctccc ccttcttctt ttttcagctg aattacttta ccatgtattt catacttttt
    18481 tcaatcggct tttttcaaaa aagtcgaatt tttggacata tcacggcaat tgtctcattg
    18541 taatctctta catattgaga aataattaag gattacagcc tttactttat catgaaataa
    18601 aagggaaaga taattttatg ccaaagtttt tatctattat acgtatacgc aaaaaagcta
    18661 aaacattttt tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt tttattcctg
    18721 tatttcttat ttttcgaatg acaggatgat tttcccagca ggaggatgtc cttcaactag
    18781 ctgatgtgct tggatgacat gcttaagtga agcagacaga ttcattgaaa taaaaacatg
    18841 aaaggctcct ttttggtagg ctttgattat gccagcaaag gcttctgcat ctagatattc
    18901 ctttcttgga acaaacgatt cataaaagcc ttcctttttt tgccgcaaat ctagatctgg
    18961 gaggtcattt aatgcaacat acctgccgcc tggtttcatg atctggatgc cggtttctcc
    19021 tttgatactt cctttcgtcg catcgattac aaggtctgcc tgatcagcaa attgcagacc
    19081 tggatttgtt ttatcataag cagcaaatgc tgaagctcct aattttttta ccttttcctc
    19141 atttttactg gatgcactgg ttaagattcg gattcccttt tcatggagaa gctggatcag
    19201 acttgacccg accgcacctg atgctcctag tatcattaca gtatctgtcg gctggatttc
    19261 cgtcaaatgg ttgattagat tataagccgt aattcctggc gtgaccattc cggctgcttc
    19321 ttcccaactc attttatctg gaatttttgc tacttttgct gatggcaaca cgacttcttc
    19381 tccgtaagtc ccgccgactg catggacagc tactcgatca cctacatgaa cagttgtgac
    19441 atctgaacca atctctgtca caataccagc gccatcatta cctggtacat aagggaattt
    19501 caatgttcgt atctctttca tttctcctaa acgcaaggca acatcataag gattcacact
    19561 gaatgctttt atttctacac gaacatgtcc agaaccgact tctctgggat gagcatcgat
    19621 ttcttcaaaa acatcttgtg cttctccgta accgttaatt gcaaatcttc gcatggtcac
    19681 catctccctt tttctttaag tttacgttta tgcctttatt ttgacaaaaa aaatgtcttt
    19741 tcgttttggc gcatgattca aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt
    19801 atttatctaa aagcgtaaag attacgattt acgctaataa aaaataattg gaggaatttg
    19861 ttgatggcta aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc
    19921 tatgctgctt tacgccagga attaaaagcg caaaaagatt acgaaggctt gcgtaaactt
    19981 cctttagatg ctcgtccaga acgattgaaa aatcgtgatt taatcgatgg acgccctcgc
    20041 ggatacatgc gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc acataaaggt
    20101 ttgattccag gagtaaaaaa agccagctgg taatccaagt ctggctgtaa tgtaaaggag
    20161 gagggcgcat ggaccactcc ttggatcatc aaaaaagata cgttcgcttc ttctagcaga
    20221 aatgctggta ctagaagcag acgtatcttt ttttacgcac agctggtcct tttttatatg
    20281 taaaggctgc atggttactt cgctatattt atttagtaac aggagtcagt gcttcgatac
    20341 ccacactaat tttcaatgct cggtcaactt ctgccataat gggtcgatcc aaatggcaga
    20401 ccttttcttt caatcttgat ttatcgatcg ttctgatctg ctccaacagg ataacggagt
    20461 ctctttctat tcctgttcca gtagctttga tcccaatgtg agtcggaagc ttaggtttag
    20521 ccatttttgc cgttatggcc gcaacaatga tcgtcgggct aaaatggtta cctagattat
    20581 tttgaataac caaaactggg cgtgttccgc cttgctccga tcccacgact ggagaaagat
    20641 cagcaaaata aatatctcct ctttttacca taggatcctc cttattcttg atattctctt
    20701 ggtatccgct gtcctaatcc gcaagccact tcatagtgga tcgtacctaa ttgatctgct
    20761 atatcctgca tagtgatttc tttgtctgca tttttgccga taagcgtcac ttttgttccg
    20821 acaggaaatt cttgaggcaa tcggatcatc agttgatcca tacatacacg cccaatcgtt
    20881 tcacagtagt tcccttctac caatagagaa aaaccctgca tctttcgtgg ccagccatct
    20941 gcataaccga tcggtatcgt tccgatccac tctgcttcag gagtgatata tgtttctcca
    21001 tagccgattc cttcacctgc tgggagtttt ttgacttgaa tcaattcaga aaccagctca
    21061 agtgctggct gcaatggata aacttccggt agtgcgtgcc cagagggatt aagtccgtac
    21121 attgctactc cataacgaat catattgccg attgtttcat catgccataa agccgttgca
    21181 ctattgctga catgtacata tcgaggaagt tcgggaagtt ttttcagcac ttctataaag
    21241 cgttcttttt gcagattcca atatgtatca tctgcctgat ctgctgtaga aaaatgggta
    21301 aataaacctt cccattctaa tacacgactt tctttgatga atgcagctgc ctcttttact
    21361 tcttcaggtg tacaaaatcc gatacgaccc atcccagtgt ctgcttttag atgaatcgaa
    21421 agaggcgtct ctagcttact tttctctaaa acagcagctg ctgcttgcaa ccattcccgt
    21481 gtacctgcag ttactgataa atcatgagca acagctacag tcagtgcgtc gtaaggaacc
    21541 atattcaaga taaggatggg ctgagtaaat ccagcttctc tcaactcgac tccttcatct
    21601 aaatttgaaa cacaaaagcc cgaagctcca cctgcaactg ctgcctcagc tgtttcaata
    21661 gctccatgac cgtatccgtt cgcttttact actgcaaaca attcttttcc ttgcggaagt
    21721 cttgcaactt catttgctac attttcagtt atagcttttt tatgtatcac tgctttcgtt
    21781 gggcggtgcc aagcgacaac catcgttttt ccttctttct agtcttccaa aataatttgt
    21841 gcaaaagctg tttcatttgt atgagtaata ctgacccata cattgccttc atgaggtgaa
    21901 cgcgtcacaa caggctgacc attttcattt tttaaaatct cgacatcttg aaaggtaact
    21961 tttccgatcc cagttcccca agctttagag aaagcctctt tgcaagcgta acgcccgcct
    22021 aaaaattcta cttggcgatg aaaaggcaag gactggaata gtttcatttc atcagaggtc
    22081 aagattcgtg caatgaattt tggtttttct tcgatcagcc gagtgatcct aggaagttcg
    22141 actgcatcga tcccaattcc ttttatcata tgcttttctc ttttcaataa atttagaaca
    22201 ctctcattct accaaattat aaccattttg ccacaaataa gtccacaaat aacaaaaatg
    22261 aaagtttctc ttaaaaaaga gaaactttca ttttatgttt aatcgttatt tgaacggatt
    22321 acaaatccgc gttttttatc attgtgacgt ttaccgccat ctttttggct gttattattt
    22381 ttgctataac cgccttttga tttgtttcca cgataattac cgccgtcacg acgattacgg
    22441 ttgtttccgc caccacgttt gccattttta ttgtatcctt ttttctgcat tggcaatggg
    22501 cgttctggtg tgatcttcac tggcactgca tcagctggat cttttgctgt cgtttttagc
    22561 aaaagtgcaa ctaaatcttg tgcagaatat tcttctaata atttatctgc tgtttgcagg
    22621 tatttatcta gaccattttc tgcaagtttt gtttcgattt gttcaactgc agcacctaat
    22681 tgacctttga atgcttcttt ttcggttggt ggacgtaaag tcgtcatccg ttttttcgtc
    22741 aagttttcaa tcacatgcaa gtagtccatt tcatttggtg taacgaaagt cacagacata
    22801 ccgccttttc cggcacggcc tgtacgtccg atacggtgaa cgtagctttc tggatcttga
    22861 gggatatcat agttgtacac atgagtgaca cctgaaatat ctaatccacg tgcagcaacg
    22921 tctgtcgcta caaggatatc taaatgaccg ctcttaaatg aacgcaatac gctcattcgt
    22981 ttttgttgag acaaatcgcc atggattcct tctgcacggt aaccgcgtgc ttctaatccg
    23041 cgtgccaatt cgtctacacg gcgttttgta cgtccgaaga cgattgttaa ttcaggtgtt
    23101 tgaacatcaa acaagcgagt catgatatcg aatttttcgt attcttttgc gcggacatag
    23161 tattgatcga tcaagtcagc tgtcatttct ttcgctttga tcttcacatg atgtggattt
    23221 ttcatgaatt taacgccaat attcttgata gctggcggca tcgttgctga gaaaagtaat
    23281 gtttgtcttt gatcaggaac ttgtgaaatg attttttcga tatcttccaa gaagcccata
    23341 ttcaacattt catctgcttc atccaaaact aatgtttgaa cggttccaag tttcaatgtg
    23401 tggcggttaa tgtggtcaag catacgtcct ggtgtaccca caacgatatg tggacgatct
    23461 ttcaagccac ggatctgacg gccaatatct gctccgccgt aaacggcttg tacacgtact
    23521 tttttgtcgc gccctaaacg atataattct tcttgtgttt ggatagctaa ttcacgtgtt
    23581 ggtgcgataa ctaaaccttg taattcgtga cgatctggat cgattttttc taacattggt
    23641 aatccaaatg cagctgtttt ccctgtacct gtctgtgctt gtccaataac gtcttttcca
    23701 gctaatgcta gtggaatcgt ttctgcttgg ataggcgttg cttcttcaaa accagcgcgt
    23761 tcgactgatt tcaacaattc tggtgataat tctagttctt taaatttcaa atgaatcctc
    23821 ctatatggtt gttaactgag gatgtagctt ttttgctacc ccgtcgattt gttcggtcaa
    23881 agtgtattgt ttctttttga cagtggccac gcacgttttt atccattctt agcccctgtg
    23941 gcaccaccga aaaatcactt tgcgaagttt aggcattaga aaagccaaac gggtcgcgtt
    24001 cagctttatc attattatga catctggctt caacaagttg tttctatggc aaaaccagtc
    24061 caatttctct tcatctttcg gcaataggtt attctaacat agtttttcgt tttctgcaag
    24121 tttacagatt tttatttcat ttgctgaagt tgttctacca cttcaaataa gcccatgctg
    24181 ttgctgcctt ttagcataat actatctgtt gcttcaagat tttgtttgat agcatcaatc
    24241 aatttattct tttctttttc aaaatagaac acaggtagtt gggggtattt ttggttcaat
    24301 tcttctttta gtgcatgcat ttgttcccca taaaggaaaa tcatggcgaa tttctcagga
    24361 tcgatatgtt cactcatttg cgcatgcata tttaaagact ctttccctaa ttccagcata
    24421 tctgctaaca cagcgattct gcgtcctttt gttggcagat ttttaaaagt atctaatacc
    24481 agtcccatgg ctgttggatt tgcattgtag acatcgctca aaatagctgc accatttcct
    24541 gccgttagcc attcggttct gttttgagtg atagaaacat gggcgagccc ttggcgtatc
    24601 tcaggtatgg acagtccaaa gaagcgcccg atcgtatacg caatcaaagc attcgttaca
    24661 ttataacttc caggcagcgg gatcgtaaat gtttctcctt ctactacaaa atcggtttgt
    24721 gcttttcctt ttgctgtcac ttccgcatga acatcgccat ttccaatacc gaatgttgtg
    24781 actgtttgta ctaatgttgc cgttaaaggt gtcagcaacg gttcatctgc gggcacaacg
    24841 agtaatccgt ctgatacaag tccgtcagtg atttccattt tggctttagc gattccttct
    24901 cgcgacccaa gattttctat gtgtgcttca ccgatcattg tgattgctgc agcttttggt
    24961 tgagccataa gtgaaagttc cgtgatttca ttggcgtgat ccatgcccat ttccaaaacg
    25021 atcttttctg tatcttgggg catgtgcaaa atcgtgtaag gaagaccaat attattgttg
    25081 taatttcctt gcgtcttata tgttttgtat ttttcagaca agacagacgc ggttagatct
    25141 ttcgtggtgg ttttaccatt gcttcctgta attgcaacga cttctgcgcc tactttttcc
    25201 aagtaatagg ttgccaattg ctgcatggct ttcaaaggat ctgctacagg aataactgga
    25261 aaattttttg gagcttgttc aacaggcaag ctccaaaatg aagcagatgc tccgtttgtg
    25321 atcgcttgct ggataaattc atgtccatct cgcgtaccag caagaggaac gaataaactt
    25381 ccttcggtta tcttccggct gtcaaattcg acattggtaa tctttgtcag tgcagagcat
    25441 tcgctgccca cggcttgtgc aatttcttgt attgttagat tcattgagat ctctcctaaa
    25501 aaaggttgtg aaaaaagcgt ttggcctaaa cggcgggttc gcatgactct cacttccaag
    25561 taaatttctc tattcgtatt tagaagctag aatgaacgcg gttacgcaat cactccagcc
    25621 tcttctttat ttctctttat tcattttttt caaaaaaaga ttgacgctga tggtatcgat
    25681 tcattcctaa ctggatcagt tcttcaatca aatcaccgta tttcaagccc atattttccc
    25741 ataagagtgg gtacatactg aactccgtaa atcctggcat agagtttaat tcattcagga
    25801 ataattcatt tttatttgtc aaaaagaaat cgcaccggct caatccgctt ccacctaaca
    25861 tcgtgtaagc taacttcgcg tactcttgcg ctttttgata aacttcttct ggcacttcgg
    25921 ctggaatctg catttcgatt ttattattga tatattttgc ttcataatca tagaatgcta
    25981 cgtcttttac gacttcacca ggcaaagtcg tccgaacgtc ttcatttcct aatacagcaa
    26041 cttcgatttc gcgcgcttca attccttgtt caacgattgc tcgagaatca tactgatagg
    26101 ctgttgctaa agcattttgc agctcttctc ggttttctgc ttttgtaatg ccgacactag
    26161 aacccatatt cgccggtttg acaaacatcg gataaagcaa agaaccttca cattgatcaa
    26221 atactttttt aggattttct ttccattgat tcttaagtac tggtacataa ggaacttgcg
    26281 gcacaccagc agcttgtaaa atatacttgg tcatgatttt atccatggca catgcactgg
    26341 tcaatacgcc tgcgccgaca taaggcatat tcaatgtctc taagaagcct tggatcgttc
    26401 catcttcccc gtttggccca tgtaaaactg gaaaaacgat ggctccttct tctttgattt
    26461 cgcccggatt gatcactttt cctgtaaagc cttcctctgt ctgtccactt gggtcccatg
    26521 aaagatgcaa gacatctttg ctggcaggtt tttctgttag taatggacct ttgacccatt
    26581 gtccttcttt tgtaataaat acgagttgaa cttggtaata attataataa atggcattta
    26641 aaactgaaaa tgcggaaaga atggacactt catgctctgc gctgcgtccg ccatatagta
    26701 aagtaatctt caaaagattg tcctcctgta ttctaaatcg ttcagtgatt tactctcata
    26761 ttttagcaca aaacaaaaat aaacgaataa cttatttata aaaaaacgag ccagcttcta
    26821 aaagaacgaa cgtcatttat tttttgtcag ctagattatt tgtcccgttt tttataaaaa
    26881 gttatgataa agaaaaggag ggatgatgat ggcgcaaaca aatccacgca gccgcttggc
    26941 agaaatcatt cgagtgttca tcaattacaa tgtagtgcca aactttgtac agcaaaaaaa
    27001 tcccgaacag gtaaaaaaag cctttgaaga actaggcccc accttcatca agataggaca
    27061 gatgctttct gttcgagaag atttgctttc ttctgccttc actcaaacat tcaaaacatt
    27121 acaagatagt gtacctagcg acacattttc aacggtcaaa aagacgatcg aaacagaact
    27181 ttctctttca ttatcagata tattcgatga tttttcaaaa tcgccttttg cctctgcttc
    27241 tatggggcag gctcatcgtg ccaagctgaa gaacggtgac gcggtagtcg ttaagatcca
    27301 acaccctaac atcgccgaag agatccgact tgatcttcaa ctatttgaac gagcgatccc
    27361 tttgatcaaa tacattcctg aaactagcgt agttgatcta aaaggggtac tccaagaagt
    27421 aaaacgttcc ttgatcaatg aaatggactt cttaaaagaa agtcaaaatg gcgaacaatt
    27481 ctatcaaaag aacaatggct ggaaagagat ccgcagcccc aagatttatg acgctttttg
    27541 ttcgaaaaaa gtgatcgtga tggaagaaat gtctggtaaa aacttgaatc atttaatgaa
    27601 cgccgaaaac aaaactgaaa cttttatcac tggaattcaa aacaagcagc tgaaacaaga
    27661 agttgcaaag cttttagttg agaactttat gaaacaggtg tttgatgatg gttttttcca
    27721 tgctgatcct catccaggga atctgctatt ccatgtacta acaaaagaag aacagacaca
    27781 agctagccga aaaactgaaa cagtccatga aaaagagttc ggttcctttg cttttcgagc
    27841 ctccacttct gcagaagacc ctgttgcgcc atataccatc aactacatcg attttggtat
    27901 gatggggcat ctttctgcag gacttaggca aaaactcaca caagccgttt tagcgctgta
    27961 tacgaaagat gcttatcgaa tcgaaaaagc ggtccttcga ctttgtcagc aagaaggctc
    28021 gtttgatgaa agtcggttcc accaagaact gacaagtttt cttgaacagt attatgattc
    28081 tcctattgat gaaatcaact tgcaagaagt attcacacat gtcgtgacga tttgtcatca
    28141 aaataatctg cagttcgatc gtgatatcac gcttttgctg aaagcatttg ggacattaga
    28201 aggggtgatc cgcgtcctcg atcctgaagt ttccctcatg gaagtcgctt cgccttttgc
    28261 tcaacattat tttctcactc atctggatgt agaagataca ttgaaacaaa gcggattaga
    28321 cttattggaa gggatgaagg ctgcgcctaa gatccctcaa cagctccacc atctattaga
    28381 aatgtggact tcgggacaag gaaaagtcaa tttggaattg aaaaagcaag acaaattatt
    28441 gagcaggatc gaaagtatga tcaatcggct tgttttcggt atgattttag ctgcattgat
    28501 cgtagggtcc tccctgcttg tccaagcagc acctgtagaa aacgcagagg tcgtttcctt
    28561 attagggata tttacgtatg ctattgccgc attcgtcatt atttttttgg caatcgatgc
    28621 attgatccaa atgtataaga agaggaaaaa gtagaaaaag gttgtgacag aaagaatcag
    28681 cttcaagaaa taagaagggg ttttacataa attgtttctc aaatttttgt gaaaattcgg
    28741 cttatttttc gaagaggcta cttctgaaac accgagaata aaaaatgtga aaaagctgtt
    28801 ctgcacccag taataagaac aatcaaaaag ggaacatgga acacatttat cctgtttccc
    28861 tgttcccttt ttgatgtatt cgagcataaa taaaatacct tcgttaccta ctatcttctg
    28921 tacttggtaa accttagtca attgcctttt ttaacgcttg acgaaaagac atatgatctt
    28981 tctccgtaaa gggttttgga ccctttgtcc ttatgccagc ttttcttaac tttccactat
    29041 gataacgttg ttctagaagc gtgccgatat tcgcttcgtc aaattctttt ccagagtgat
    29101 ggaacactcg cacacccttc ggcaatacaa gtgaagctaa accataatct tgcgtgatca
    29161 aaaagtcatt cttcttaacc ataccgacga ttttataatc cgcactatca gctccgcggt
    29221 ctacgtagac gatttggaca tgattagcgt attctttggt cgtataatgg tccacactgg
    29281 tcacgataac cacgtcaaga ccataggatt ctcctaattt tatgacatct tcttttactg
    29341 gagaaccatc tccatcaatg aatagcctca ttctttttcg ccttcttcca taaatgataa
    29401 acagttacta atccgctcag taaaagacca acattgatcc attttgccaa gataagcagt
    29461 tgtacacttt ctatcggcac tgattcatgg ttcagcgtga tcaataatag atggattggc
    29521 tctcccattt cttttgctaa aacaaacaaa agcgtcatca ataaaaactg gtggatcaca
    29581 ctccctttat tttgagtagt atacttgaca acgatccggc gatccattgg aatagctgta
    29641 tcagtttgat aatattcctt atttagccag ctgcctgtta ctacgtgcca gaaactaagc
    29701 agaaagacac ctatcgcaaa accatttgga atcaagagga ccaataaaga agaactcagt
    29761 ccaagcaatt gcatagccaa tggagaccat gttaaacgtt tcaaaatcca tggtccgact
    29821 atttttgctg caatcaagcc tgctgcataa ggcaaataca taaaaatcga aagtttttct
    29881 aatccgtaca gtatgccaac ataaaaactg ctaaataaaa aaaggaaatt ccctagcatt
    29941 ccatttagaa aagtaaacag atttagccag cttggcaact tccattcctt cttctggtgt
    30001 cctaaaacaa acacgaacaa gatgaacagc agcaagaaac cataaatagc aagatcaaaa
    30061 agcctttcat cgaataacaa tcgggcattt ctcaaaagaa acaataagcc aaagaatatc
    30121 aaaaataaaa caagctctcg cgtagctacc agatttcttt tgatgtcttt gaaatccagt
    30181 tcgtaacgcg gataatgact aaccgtatgg taggccatga cgtaaaaaag tgtataaata
    30241 gtaaagcttg ctgcaatctg agtcggtgca ggcaactcaa tcgcttgata caggagaaat
    30301 agccagatag ctgcaaataa gtactttttc ccatccatat tgataaaacc ttgcgtcttt
    30361 tcgtgaaaat tgaccgtggt attagcaggt acaagccatg ctgcactcag ccctagcaaa
    30421 acacttgaaa aataaaataa aggaaaataa aatacgccta atattccaag caatgaacca
    30481 gcaccaccaa tcagcagagc gatcatcagc aacgtgtaac tatctagacc aaatcgtaga
    30541 ctgttgacta aaaatagtcc agtcatccga aaggcataaa acagcgtaaa tggcaaaatc
    30601 gtcagccaat tatgcgagtc tgttagtccc aaaaataaaa gataaggaat aaaaataacc
    30661 gtgttactta agataaaggg cgttgtttgt gtaaaattac gcccaaatcg tttgagtttt
    30721 tcgaacatga caatggctcc ttaaacagtc ttataactaa tctgctcttc gattttagca
    30781 ttaaagggga aataaaccaa ttttacagaa tatattcttg taaaaaaaaa tacagaaaga
    30841 agcataagct tctttctgca tttttacggt acttgattac tcaagcagac aagcccttct
    30901 ccacaaaagt cttgcccgac acccctgcat ccggcaggtg ataaagatag aaatcaatta
    30961 tcctgtgcct ccaaaatcaa ttaggagctc tgtaaaccag agtccttctc gacccgtcgc
    31021 ataaatttac tttaacacag ttcctatgtt tatgcgagag cttttttcat tttataatcg
    31081 accatttgaa atccttgacc gatatctgaa ataagttctt ctgtaatctc aaatccttca
    31141 tgcttataaa cctcgattgc ttgatgattc ccctgattca ctcgcaaaaa gatctctctt
    31201 tcctttgttt gcgcagcaag ttcttcatac cagcgaaaaa ctgaagaagt caatccttga
    31261 ccacgtactt tatttagcaa atataattta cttaaataaa tttttccatt tatctcttca
    31321 taagcagtgt aaccgacagt cttttcttcg ctttttaata aaaagtagga cacaccttct
    31381 gtcagttctt tttgaatctg tttttttgac tgataagttt tcagcatgta atccacttgt
    31441 ttttgaccaa tgatcggcac aaaaacttcc ggccatactt cttgtatgac tgccatcagc
    31501 tcatccagtt cattttctgt tacttttatt ttttccatac acccacgtcc ttttttacat
    31561 tataaatgac cttgcgcttg atggaataaa aagagcatga tcatgctata cacacccacc
    31621 gaccacggtt tcaagaaaat cacaatctgc ataaattccc taaaagataa tttcgtcaaa
    31681 ccagctacta gacagatcaa gtcgtctggc gcaaagggaa gcagcaaggt aacaactagt
    31741 acttttttaa tatctttcgt ttttcttgtc agcaattcat caaatttctg atactttttt
    31801 ggcgataaaa taagctggac aaaggctacg ccatagtaac ggacaagcaa aaatccgatt
    31861 gcttccccaa tcactaggcc aatacaacta tataaaagcc caaatccatt gccaaacatc
    31921 agcatgcccg ctactgagga gatccctccc ggcaaaatag gaatgatcac ttggatcact
    31981 tgcaacagaa caaatatcat cactgcatac ttgccaaatt gctggataaa cgcttgcaaa
    32041 gacgtgctcg atcgaaaaat cccatgatga taagcataaa gaatcaacac aaagaaaaaa
    32101 aacagaccaa taatgggcat gatctgaaga catttttgta aaactgatcc ttttttagac
    32161 atctatattc cccacatctt ttttcattta ttaaaactgc ctcctt
//
LOCUS       DAHHPA010000022        31073 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.023, whole genome shotgun sequence.
ACCESSION   DAHHPA010000022 DAHHPA010000000
VERSION     DAHHPA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.023"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            81..950
                     /locus_tag="NBH55_000932"
     CDS             81..950
                     /locus_tag="NBH55_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCD1508936.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            970..1317
                     /locus_tag="NBH55_000933"
     CDS             970..1317
                     /locus_tag="NBH55_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508937.1"
                     /translation="MEIISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1330..2592
                     /locus_tag="NBH55_000934"
     CDS             1330..2592
                     /locus_tag="NBH55_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508938.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2672..4048
                     /locus_tag="NBH55_000935"
     CDS             2672..4048
                     /locus_tag="NBH55_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCD1508939.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            4045..4674
                     /locus_tag="NBH55_000936"
     CDS             4045..4674
                     /locus_tag="NBH55_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508940.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4693..5172
                     /locus_tag="NBH55_000937"
     CDS             4693..5172
                     /locus_tag="NBH55_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508941.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5674..5727
                     /locus_tag="NBH55_000938"
                     /pseudo
     CDS             5674..5727
                     /locus_tag="NBH55_000938"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5731..6330
                     /locus_tag="NBH55_000939"
     CDS             5731..6330
                     /locus_tag="NBH55_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCD1508942.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIHFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6346..6822
                     /locus_tag="NBH55_000940"
     CDS             6346..6822
                     /locus_tag="NBH55_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508943.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            6839..7741
                     /locus_tag="NBH55_000941"
     CDS             6839..7741
                     /locus_tag="NBH55_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508944.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7814..8008
                     /locus_tag="NBH55_000942"
     CDS             7814..8008
                     /locus_tag="NBH55_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508945.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            8113..8541
                     /locus_tag="NBH55_000943"
     CDS             8113..8541
                     /locus_tag="NBH55_000943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508946.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNKETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            8776..9363
                     /locus_tag="NBH55_000944"
     CDS             8776..9363
                     /locus_tag="NBH55_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508947.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            9363..10262
                     /locus_tag="NBH55_000945"
     CDS             9363..10262
                     /locus_tag="NBH55_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCD1508948.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            10262..11176
                     /locus_tag="NBH55_000946"
     CDS             10262..11176
                     /locus_tag="NBH55_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508949.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESGMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            11228..11443
                     /locus_tag="NBH55_000947"
     CDS             11228..11443
                     /locus_tag="NBH55_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508950.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANRLY"
     gene            11633..11887
                     /locus_tag="NBH55_000948"
     CDS             11633..11887
                     /locus_tag="NBH55_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508951.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            12066..13115
                     /locus_tag="NBH55_000949"
     CDS             12066..13115
                     /locus_tag="NBH55_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCD1508952.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            13294..13524
                     /locus_tag="NBH55_000950"
     CDS             13294..13524
                     /locus_tag="NBH55_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508953.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            13527..13784
                     /locus_tag="NBH55_000951"
     CDS             13527..13784
                     /locus_tag="NBH55_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508954.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            14001..14876
                     /locus_tag="NBH55_000952"
     CDS             14001..14876
                     /locus_tag="NBH55_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508955.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQENLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSEKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKEE
                     ITKMKPVIKILDNKE"
     gene            14886..15680
                     /locus_tag="NBH55_000953"
     CDS             14886..15680
                     /locus_tag="NBH55_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508956.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNNGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            15709..16827
                     /locus_tag="NBH55_000954"
     CDS             15709..16827
                     /locus_tag="NBH55_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508957.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKVKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTVNEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFAKSKDEVKGVVIDDDFEFDFD"
     gene            16860..17180
                     /locus_tag="NBH55_000955"
     CDS             16860..17180
                     /locus_tag="NBH55_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508958.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            17437..18126
                     /locus_tag="NBH55_000956"
                     /pseudo
     CDS             17437..18126
                     /locus_tag="NBH55_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
     gene            18163..18330
                     /locus_tag="NBH55_000957"
     CDS             18163..18330
                     /locus_tag="NBH55_000957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508959.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            18350..19978
                     /locus_tag="NBH55_000958"
     CDS             18350..19978
                     /locus_tag="NBH55_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508960.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            20029..20397
                     /locus_tag="NBH55_000959"
                     /pseudo
     CDS             20029..20397
                     /locus_tag="NBH55_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20497..20697
                     /locus_tag="NBH55_000960"
     CDS             20497..20697
                     /locus_tag="NBH55_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508961.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            20831..22057
                     /locus_tag="NBH55_000961"
     CDS             20831..22057
                     /locus_tag="NBH55_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508962.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            22907..24232
                     /locus_tag="NBH55_000962"
     CDS             22907..24232
                     /locus_tag="NBH55_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCD1508963.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            24225..24572
                     /locus_tag="NBH55_000963"
     CDS             24225..24572
                     /locus_tag="NBH55_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508964.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            24887..25744
                     /locus_tag="NBH55_000964"
     CDS             24887..25744
                     /locus_tag="NBH55_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCD1508965.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            25764..26324
                     /locus_tag="NBH55_000965"
     CDS             25764..26324
                     /locus_tag="NBH55_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HCD1508966.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            27290..28057
                     /locus_tag="NBH55_000966"
     CDS             27290..28057
                     /locus_tag="NBH55_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HCD1508967.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            28083..28628
                     /locus_tag="NBH55_000967"
     CDS             28083..28628
                     /locus_tag="NBH55_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508968.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFDGQQIGLKKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            28629..29195
                     /locus_tag="NBH55_000968"
     CDS             28629..29195
                     /locus_tag="NBH55_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508969.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            29210..29458
                     /locus_tag="NBH55_000969"
     CDS             29210..29458
                     /locus_tag="NBH55_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508970.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            29478..29717
                     /locus_tag="NBH55_000970"
     CDS             29478..29717
                     /locus_tag="NBH55_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508971.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEQLLRKAQQKGYPDKMLKQIGYYT
                     PEERIEKESCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            29734..30318
                     /locus_tag="NBH55_000971"
     CDS             29734..30318
                     /locus_tag="NBH55_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508972.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEQELILERQSILCSEQDSKRLYGLYDLQLNGLKAQYV
                     ADSFDDMVGQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSIVES"
     gene            30425..30706
                     /locus_tag="NBH55_000972"
     CDS             30425..30706
                     /locus_tag="NBH55_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCD1508973.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFNEA"
     gene            30696..31037
                     /locus_tag="NBH55_000973"
     CDS             30696..31037
                     /locus_tag="NBH55_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD1508974.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWVDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIERLQRKV"
BASE COUNT        11895 a         4018 c         6270 g         8890 t
ORIGIN      
        1 aaaatacatt tgacaaaatt tacaatatag tttattatat atttatctta ttatgataac
       61 acaataaaag gaatgatggt atgaaaaaga agaagtttag tcgatcgttg ttaggaattt
      121 tagttttagt tgcggttggt cttagtggca agggttactt tcagaataac agcactcagg
      181 aacatgattt tcagatcaaa agtagtgttc aggtaggtaa taaagcagac agtgaatcaa
      241 aagaattttt agggatacgt tatgatccaa acaaatatcc aaataatatc gtaaacatta
      301 atgatgggaa atatacactg acaaaagaag accatgaagc attatcagaa aaaggcagtc
      361 aaaccttttg ggtaaattat gcagatttag atcaacttgg aagagcagga gatgttgaag
      421 cgttgatcac ctatgatgct gtaaattctc actcatctaa aaatgtggaa cgtccatctt
      481 tcaaaagttc tgtccatatt gcaggagaat acaaggatgg agtatttgat caagagagag
      541 atacttgggt cggcaaagat tcaaacaatg aaatcactca gctagaagga tataaaggtt
      601 atctctataa caaaagtcat tcattagcat ggtcgctagg aggagatatg gaggtacaca
      661 atttaacact tggaacgaga gcgcaaaatg ttgggaaaaa tgatcagaat ggagggatgg
      721 cttactcaga aacgattgta cgtgatgcaa tctataaaaa taagaatgaa aaagtattgt
      781 ataaagttga tccaatttat aaagggaatg aattggttcc tcgtggttct catgtcgaag
      841 ctttctcagt taacgataat ggaaaaacgg taaaccttaa tgtttatgta tttaacaatc
      901 aaaaaggaat caacatcaac tatcaaaatg gtacttgggt aagagattag aaattgagaa
      961 ggtgaagaat tggaaataat aagcatcaat ggattagata aatcagttgc aaatcgaata
     1021 aaaaaaggaa atggctacta tcttctgaag aagggtaaat attcagttgc tagagagtta
     1081 gaaacaatga gtatcgtgta tgtagctaag aatcattttg acgtagaaca ggctattttt
     1141 gatccagatt ctgaaatagg gaaatgtgtt gaattatcag agaaaactgt tagtacaaaa
     1201 ggggcgatga tcattgagag agaaatgtta gacgaaatca gagtaatcat tcctaaagat
     1261 acgcagatgt gtgaggacag cataagtgac gatgaaccaa aatcagaaag aaaataagga
     1321 gatattcaga tgagcgaaaa atccattgaa ttaaaggaat ataagcgaag cgatggagaa
     1381 aaatattatc ctgtagccag tttttctaaa tcagtatatc tggttggacg tgcattggta
     1441 gttggtgcaa ttggcatata tattttgcat tgggtaacaa aggttgtaca actgcttgta
     1501 gataccttag taagtattct tgtctcgtta aatgatgtta acggaacaga tgatttagga
     1561 ttaaaagaaa tgtttaaaga gttactcaat gcgttttttc ccaattattt aagttattca
     1621 tataatgtac aaagcttttt tgcaaagcta cacttggtag acatctctat tttagtatta
     1681 gaagcaggaa cggtattctt tgttggatat ttggttactt tgatgttcag gcatcactat
     1741 ggagaacaaa gaccattttt tgatgatatg gaaagtagaa ggttgaaaag agaagctcta
     1801 gaagctatgg atgtgaagta tgatccaaac aagattatag gagatgaaac gaacaacaaa
     1861 aaaagaaaac gaaacaaaac gcaattagaa tccgatgtgc tatcaacgct taaaaatatc
     1921 aaaatttctg ttcatacaag acgtgatcta agtgtcaatg aagatattcg agaatatatc
     1981 gtaaaaatcc cgcaaccaaa agggaagaaa gttagagaaa cagttttacg acaaattcaa
     2041 gatttagggt cgatcctgac aagagcaaca agcggacgag ccaaatttgg tgaaaaagaa
     2101 gatgaagttt caggcgatta tttcattttc cgagcatcag taaatattga aagagaacgt
     2161 aaggaaagag taagtaccaa aaacaaagaa acggaaagag caacatcttc aatcgcagtt
     2221 tcaaattcac aattttcttt tccaatcaat ctctttgccg ataattccaa aaatatccaa
     2281 aaccaaaccg aaaaagctag aaaatatgcc gaaactcaaa agcaatcttt agaactttat
     2341 ctatcttctg ctgacgtacc tgcaagtcta acagatatgc acgttgggaa tacatctatt
     2401 gaatatttgt ttactttatc tcgtaccaga aaaattcaac taagctcaat gaaagaagaa
     2461 attgaaactt acttgcatat atcaggtgta atcgtgcgga tcaacgcagg gcgtattcaa
     2521 attactttac cattaggaga aaatgatcgt attccaattg atgtaccatc aatgatcaat
     2581 agcgtgtttt aaaagacatc acaaatagaa acagaaaata caaactacca gttcacaaac
     2641 agaaaagtac cgagaaaagt gaggaataga aatggctatg aaattattag agaaagtcaa
     2701 aaagagagcc gaaaaagctg aatctatttt gctagataaa gaacctaata taaagcttaa
     2761 tggaaaattc agtgtcaata tgttgaacac aaaggctaaa gacccgacag atgctatttt
     2821 tggaattaag cctgatggca caattgctca tttttgcatc agcgaaatgc cacacatgtt
     2881 ggttgcagga acgacaggat cagggaaatc agtattactg aatgaaattc ttgttacagc
     2941 aatgtgtcac tcaacgcctg atgaattaaa aataggaatt gtagacccaa aaagggttga
     3001 atttggaaga tacaaaaaat tgccgttcat gttggctgat ccaattacag atatggatga
     3061 agcttatgat ttttttgaat atcttgtgat cttgatgcat gaacgttaca aactgatgga
     3121 aaaagcaggg ttacaaaaca tcacgctata taacgaatat gccgaaaaaa atggattaga
     3181 tagatttccc tatgtgatct tattagttga tgaatattca cagttggttg gaacacataa
     3241 agaagtagaa ggtttgattg ttcaattagg acaaatggca agggcagcag gtattcatgt
     3301 tatcttagct acacaatctc ctcgatcaac agttgtcact ggtattatca aagccaattt
     3361 tccgtcacgt gtttgcctta tggtagcaag tgatttagaa agtaggatcg tattagatga
     3421 aggcggtgga gaaagcctaa gtccaaaggg agatatgatt attaagctcg ttaatggaag
     3481 tatggttaga gcgcagggag cttacatcag taacaaagaa attgaaacaa ttttcaatca
     3541 ccttagaaat actatgccag aaccagaata tgttgattac cgagcaatcg tagctgaatc
     3601 tcgtggtgag ggtggagaag acgaggattt tggtggaatt ggtaaagctc ctgtaagaaa
     3661 atctgctaaa agaaatttga acacaccttt agcaaattca tcatctaaag agccatcaat
     3721 tgaaaagaag agtacgtttg ctctaaaact tgaaaaaaac aaagaagaaa aagaaaaaca
     3781 aggagctaaa acactagaaa aacttcaagc cgaaaaatta gaaaaagttt taaaagcatt
     3841 agataaagct gattctgaac cgcaaacctc aaacaacacg aataatgaac agccgatttc
     3901 atctaaaact aaaccgaaaa ctactgatcc gaaagtttca gtaaacgtga ccatcacaac
     3961 cgataagtct gagaagaaac aagtaaacgg aacgacttca cctatcatgc aaaaaatgtt
     4021 gaaagccaaa aaacaggtgc ataaatgatc attgtcagtt cttttttgag gtggcttacg
     4081 ccatataaat atacagatga agtagttgtg aaacgaacga agaaaagaga taaaaaagcg
     4141 gtactaactc ctaaacagat gcttgatcat ggcattttag gggtgaaggt tttagaagtg
     4201 aataaaccgt ttgaatcaaa tagtgcaaga gaaaagttgc ttggagctga agccgatgtc
     4261 gcatattttt accatgacaa aagcaagcat caaatagcta ttaaattcaa tccaaaatcg
     4321 gcatggcggt atcaagcaag agatgacttg aaacgtagga aacttagacc aatgctctat
     4381 ccagaaggaa aagaagcatt tgatagaacc cacatcgttc ccattggttt tcatggtaca
     4441 gaatcagata accgcctatt agtaggttgg aacagcaatc aaaatagagt tcagatcaat
     4501 aaatttgaga agaaagtaag gaaattcaac gatcgaaaaa cgatcctatg gtttgcagat
     4561 atttgtaaac agccggatgg aacagcaaag tgggaagcgg tgatatttga cgaaaatggt
     4621 aaaaaagtaa tgtctgaaac atggcatgat aaagctaaat ttgtttggca gtagcagtag
     4681 aggtgaaaaa gattgctaga actttttgaa gcaaatagaa caggctttaa tcaaaaatta
     4741 gaagtgggag aaactataga atataaagat aaaaactata ttattatcgg aatctataac
     4801 acgagaatta acagaatagg tgatccacgt ataaccagtg acttggtttg tcaatctgtt
     4861 aactattcac ctgatctgac aagcaggtat aagaaatacg tgttattaga atatagacac
     4921 aagattactg atgaaattgg tgtaaacaac acacgaaaca tatttaagat cggaacagtg
     4981 attccttata gtaacaacga agaaaaaata ttttatcaga tttacggaat agaaaagttt
     5041 gaatatgaac atgtcgatct gctagtgacg taccaaatgc gtttaataga accttggtca
     5101 caggcagaaa ttgataaagc agtaaaattg aaccgattat caaaatttaa cgtattagga
     5161 aatgcctttt aaaaatcgca atacacaaac agaaaaatac caaaaaatta gcaatgaacg
     5221 taggtaagaa catgacaata aataataata accctaaaaa atataatgct cttgtccaat
     5281 gctatatcga aaacaaaatt acaatgcaag aagtcgctga tgaattaggc aaatcaaaga
     5341 gcctagtatc acacgatttt aagcacaatg tttttccgaa cccgattgag ctgaggaaaa
     5401 aatttcaaaa gagagtttat caagaaatag gtaagtggat tgatgaaggg aatgcaattg
     5461 accctttaat tgaaaaatat ccttttttaa aaagcaaaaa taaaattcaa aggttaattc
     5521 gtcaaggcga tattcaagct aaagaacctg ttttgacaac caatgatttg gctaaaataa
     5581 atcttcataa acaaattatt gaagactttg aaaaaaatct ttcaacaact gaaattttaa
     5641 aaaaatacaa tattccgaga ggaaagctaa catcgactct ttttagagaa tatggagaat
     5701 cggcagttac taaagcaaag gaagtagcga atgagagaaa aaaacaaaaa tagtagtcga
     5761 tacctagtag cgactgtagt ttttgtttgt gtattgattt tcctgtttga ttttttgcag
     5821 attggaaaaa gcataattgg aaatacattg agtgcaaagg aaatggttgc tttaggtggt
     5881 ttgtatgaag gacacttgtt cccgaatata ttcatttcta tcgtttctca tattaacttg
     5941 taccatttaa tttctaattt attgttctta gtagttcttg aagaagcact gaatgatcag
     6001 tatgagtggt taacattatt gatcgtttta atcatatcag gatttctagg aagcttagtt
     6061 ccttttctgc tacacagcaa catcgtaagt atggggctta gtggaggtat ttttggatgg
     6121 attgctttat ttgcgattgc atacatccat tttgatagca agaaacattt attactaagt
     6181 gtttttgtat tattgataat cggcatttta ctggtatcta cttttgaatc aggcgagaca
     6241 aacgtcttgt cacatgtaat cggatttgtc tgtgggatga tcttgggatt tattctaaag
     6301 aacgtttcat tgatcaaaca aaagaattaa aagggagtgc taaaaatgcc aaaaaaaaaa
     6361 gaaaagaaaa ttccagtgtt tttgtctgtc tcagaaatgt tatcacttca aatactgtta
     6421 agaaaaaaga ttgaaacaat agacgaagaa gttagaaata aaagggctat gtacgaacaa
     6481 cgtgaagcgc taagagaacg aatccactat tttgatgata tacacactaa aatctatgaa
     6541 gcacgtacaa atcacgaaga cgaaagcaaa ttaggattag ctcaacaacg aatatttgaa
     6601 tggctgaata cgaatactga gcaagaaccg ttaagagcaa ttagtaatct ttttgaattt
     6661 ggaacgaagc attggtttaa cgctgtagaa aaggatgctg acttacaaga agcgttcgat
     6721 acactaagcg aggatagcaa atccatgcga gacttattga tcacttatct aaatgagcat
     6781 gaatttgaac agacgaaagt taaaaagaaa aataaaaaat agaaagttgt gatgtatcat
     6841 gctgtttacc ttattaggtt atttatgttt agttcttgct ttttttggag caatagctat
     6901 atttgtcggt cagtttcaag caaataatcc gataatgcgt aacataggca ttggaattgt
     6961 tatcatatcc ttgattgtat ttggtgccac aaagattttt gggagtaatg atcagcagtt
     7021 tggtatttct ggagttgata atagttatgc tccagattca ttgggaagag tagtgataga
     7081 tggaacgttt gagaatgtaa cgagctacaa aaatgtttca ttgacagtga aagaagatga
     7141 caagaaagca tcagatgacg ttctcgctac gatcaagccg tctcaatcac agtttgaagt
     7201 gttttataca gtcaaagaga ataaccctgt acgcaagcta acattagttt ttaacgttga
     7261 cggagaagaa tactataaag atgtaaaaat caataaagca tcaacgaaaa agattgtcga
     7321 atcagcgaaa aaagaagtag ctacagaaga aagtacaact gaatcaagtt ctactgtcga
     7381 agttgaacta ccagataaaa ttgattttga tcctgaaaaa gtcaaatcgt ttgaatctgt
     7441 ttctggttct atggcagatc ggtttaacac tgaagttttc aaagctatca aaactgttaa
     7501 attggatact gaaaagcaaa ggattaatgt taccttattc aatgcagttg aatctatgga
     7561 tcaggataaa aaagttacgg ttgcaagaga actagggaaa agagtaattg ggttgtatcg
     7621 aggtacggca aaaccagatt cagacggttc agatttgcca gttcacgtag tttatgaaga
     7681 cggtaaagtt tttggagact ctacagcaaa tgaagcacta acgattaaat tagctaaata
     7741 gccggaaaat tataataaac aagtggtgtc acaccaccat cgcataggtg tgacaccttg
     7801 gagatgatta ctgatggtag taaataaaga aaaagtaaga aagtatgtta ctttaaaaat
     7861 agcttctgtt gaatatttag aaaagcgagc taaagaagaa gaaaaaacaa gaggtaagaa
     7921 ggtaacagtt tcagagttgt tagaagaatt aattgagagt gaccaaacaa taagacgtgc
     7981 atttaccaat aggtctgtta aaagttaggg tgatactatc tttaatttca accaaatttc
     8041 taatatatga tgaatagttt ccttgtacaa aatatctaat taagagataa aagttaggag
     8101 gattcattca aaatgattat ccaaaagttt gactggaaaa tgaaattagg aattagtctt
     8161 gtaatattgg gatttattat ttttttgggg ataaaagcac aaaaaaatca atctactttt
     8221 gaaactatgt cttctcaaat tattgaggaa tgttccaaaa tttattattg tgagaacaaa
     8281 actgaagaat ctaatggctt ttattatatc gatcaaccac atgatgttgt tataaaaatg
     8341 gagtcacatg aagtaaacga atggattcaa agaagtacta atgtatcaaa aaagttagaa
     8401 agatattctc aaacgttttt aagtaaagtc attttttcaa attgcaaaaa taaagaaacg
     8461 tctcaaagag tagctagttc tttagaaaat ttaaaatttt ctgtttcaaa aaaagagaaa
     8521 tttgtagatt tacgaaaata aactatcgaa agtagtatga ttatgatctt aaaatatcat
     8581 cttaagagaa gaattacgat tgtttatagg atttacaaaa tgaataagtt tgaagagtga
     8641 tttggtcgca gactagatca ctctttttgt ataaaaaaac tttacactta tttttgagtg
     8701 tgttaagatt ttttatatag agtgacggtt tcttgtcaga gtgaatgata accatcaata
     8761 ttggaggaat aataagtgga aaacaataat gatattagat attttagcat tggggaaaga
     8821 gttacaacag taagtgaatt atcaaaggat tatagagata aagaaggaac gattatttct
     8881 cttaaaaaga atcgaagagg tgtttggcaa tatgttgttg atttagggga tggcgttaca
     8941 gttgagtgta aagatagtca actgatcaga aatgatggca gtcattccat tgaaaaagca
     9001 attcttgtat tcccaaaagt atcaagcaag attatgaatg actggttgct tggaaaattt
     9061 ggtgagtaca ataaaaaaga aatcgcagaa gtgatcggca aatcttatcc aacagtatta
     9121 gggtatctaa aaaagataac gaacaaagaa gcgaaagttg aggatgtttt aacagtaaga
     9181 gaacttcagt cattaatcga atatcggaat aaacaaataa caaatcctga gtaccttaaa
     9241 aaagaagatg gtctattgaa acaattggaa caggtaaata aaatccggaa aatgcaaaag
     9301 gtagctcctt taagcctaga taactttaaa aaaatctatt tgaaagagat cgaaaaagac
     9361 taatgtggag tgtcacttta ccgtttcaac ctgtatctgc accaagacca agtgcaagaa
     9421 ggaatgttgc tgatgcaact aatgacgtag cagagaaaag gatttcaacc tattatgata
     9481 aaaagtatct cgattatttg aaaaaaataa aaagctttat cgaagatcaa ggtttgttag
     9541 atgatgaatt ttactcgatc gtaaatgatc cgatgggtgc aattatggaa gtggactttt
     9601 actatcagat accaaaaagc tataaaaaag tctataacat tatgaagacg acagcgccag
     9661 atttagataa tttagtaaaa agtgctatgg atggtatttt caacatctca gctatacgag
     9721 atagccatat cacagggtta attgctttta aatacaatgt ggcaaacgaa ggaaaaacag
     9781 ttgtgaggat caagcgatat tcagaatttg agtgggatac ttctgaagga ttgaacggag
     9841 atatttggga agccacattt gatttcaaac cagtagcaac gccaagacca aagtctaaat
     9901 ttagaggaaa tgggattatc acttactatc caaaagagta taacgactat ttggaaaata
     9961 tcaagaatac gcttaaagag aaagatttgg taaatgatca actgaaaaag gtaatgaatg
    10021 ctcctatggg ggttatagct cagatagatt atttttacca agtgagaaaa gataagaaaa
    10081 agcttgattc actgatgcgt acttcacagc cggatattga taacttagtc aaaggaacat
    10141 tggacggaat ttttaacaag gaaattggca tcaaagatag cagggtggta ggtttaatag
    10201 cttttaaata caatactttt gaaaagtcga aaacaaacgt gcgactacag gagatgggct
    10261 gatgaataat aacgaaaaaa ttcttcatgt acttgatagc tttgaaacaa ttcaagagga
    10321 actaaagaaa tatcgagatg ttttggaaca aagatatgat tttgtcaatc agcaaaagag
    10381 taatcacatg gatttcattc tcaatatgaa tgacttgaag aagaaattag ttgaacgaaa
    10441 agaacaagaa aagttgatca aagcttactt tgaactcggt gaaaaagaag ttaaaaaggc
    10501 aatggaactg aatgaagatc gcagggtttt agatcagctg ttagaacaat tattggtgat
    10561 gtttcaaaaa ggacgaatag atgaggattt aattgaagaa gggttgagaa agtatcctgc
    10621 aaactcaggt atcggaatag tgctgaaagc gattgatgaa gaagaaattg aagatttcat
    10681 accggcagag gattttgaat cgggaatgga atatataaaa tattacagcc aagggatcac
    10741 tgcttttaga gagtttgatc ctgaagatgt tattcatgac ttgaacaacc taaaagaatg
    10801 gtgtgaaggt tatgaagttg atgattccgg tttggactac ttgatcagta ttatggaaat
    10861 cgaagaagag atgccggata agcctgatcc aacagatatt ttagaattaa tccatgatgc
    10921 tagaaatcca attgcataca tttctagagg atatactgta ttggaatact ataagcctta
    10981 tatatcagcg atgaaccatt taagacgtgt attaagggaa aaacgtgaat accgctcagt
    11041 gttaaatgca tcgaatagat tagaaaaagc tgtttcagag ctagatgctt attatcgtga
    11101 acattacctg caagcaggtg gtatgccaag aaatacgaaa gcaaatatca gtaggtatat
    11161 aaaagaagct gagtaggcgg agttagccca taaaatggca aaaggtaacc tgaaaggggc
    11221 ggtttgaatg aaaagggatg tttttgaatt gtacgtatta gaacgtctag aagacggcat
    11281 gatggcacaa gagataattg atgaaattga tgatatgatc agcagatttg gtcaattgga
    11341 attgaatgat ggaaacaaaa attcggtagt gctaaaacaa ttttggttaa ataatttttc
    11401 gtataaggat tttatcaaag aaaaggcaaa tagattgtac tgactaaatt cacgaggatc
    11461 agtgatgaga gcttattgct aagagcagtt aggctctttt ttatttgtgt aaaattattt
    11521 tattttacaa tacacattta tacacaaaaa atgttgacgg atagtacaca aatgtggtag
    11581 gatatatttg taagcaagtt agattaacaa gttaaaggaa ggaagagcag ttatgaaaaa
    11641 agaagaacta atagatatgt ttcaaattgt tgaacgagca aataatatgg gaattatgtt
    11701 ttttgatcgt atttctttaa agatggattt aagcgttgcc caccaagaat ttaatctacg
    11761 attaaaagct ttattgattt ctgatgatgt taatttcgct catgatgttg taggtataca
    11821 gaaccatata gatagagaaa acaaacgtat gggtgatgga ttcttaccaa gatattcaag
    11881 tttatgattt ggcgaaagat tcaagagtat gtgtcattat tgataggtac tcttgaattg
    11941 gaaaggagga atgaatttaa gacaacaacg attatgatct taaaacaatc gtttatgtaa
    12001 ttggcaatct tacgaataat tttcatgcaa taatgacaat aaaaaatgaa aaggggtaaa
    12061 atcaaatgaa aaaattttta gtaggaattc tattcacaat ggggctagtg ctattatcac
    12121 cttcagtttc agcagaaagc tctatttcca tgtatcgtat gtataatccc aatagcggag
    12181 agcatctata cactagaagc gttggagaaa gagacaatct gaaacgttat ggatggcatt
    12241 acgaaggtgt cgcgtggcaa gctccaacaa gtggttcccc agtttatcgc ttgtacaaca
    12301 gatataacgg tgaacatttt tacaccgttg atgccggaga aagagataag atttcgcaca
    12361 caggatggac gtacgaaggt gttgcgttct attcctacaa cggagcaaat agagttcctg
    12421 tgacacgctt gtacaacaaa tacgtgaatt ggcaccatta cacgttagat caaaatgaaa
    12481 agaacgtaat tagtaaacaa ggttggaaga ttgaaggtac gggttggtat gcaatgccaa
    12541 acagcgcacc aaatcctaca cctgcacctg ctccaagtaa tgaaaaagtg ttaaatgttc
    12601 ctgtcatttg gcaaggtaat acgatgttgt gtgaaggtgc ttccctgtta gagggattgc
    12661 actacaaagg agtagcgaca aatcaaaact taaccagttt tgtaaactat atgccaagag
    12721 ctaatgacaa taatccatat catggctatt ctggcgaatg gcgctacaac gtaaatggta
    12781 cgtatcaagg catgatgcct gatccagtag ttaaatgggc aaaaaatgtt ggtggtaatg
    12841 ccgttaatat cactggttca ggaacgaatg ggatcaagaa tgaaattcgc aatggtaatc
    12901 cggttgtaac ttggattact tataactatg cttcacctca attcaaacaa atgccttggg
    12961 ggcgtgcagt ctggaatgga catgtggttc tcgtggatgg cttcaaaaat ggtgcatacc
    13021 atgttgttga ccctgtattt ggcgcaaaat ggatcaatgc aagcacgtat gagagagcat
    13081 ttaatgtgac tggaatggcg gttgctgtac gttaattgat agaattaata aataaatgaa
    13141 ataagagctt aattactgaa atggtaaatg ggctcttatt ttttagaaaa aaactgtttt
    13201 tttgtaatac gaatctataa caaaaaaacg ttgaatgtag tacacaaaag tggtatatta
    13261 aaaccgtaga gattatgcga agaggtgatt ttgatgaacc aaagaagaag aaaaaggcta
    13321 gaagaaataa gcgagcgact atccgagtta aaggatgagt tagaagtaat tcaagaagaa
    13381 gaggaagagt acaaagaaaa tatacctgaa aatctacagg gatcagaaag atatgaaaaa
    13441 gcagaagaat ccgttgatca acttgaagaa gctgttgatg gtttatccga catattagaa
    13501 ttgattgaag atatagctag ctagagatga ttaatgaaga tttcaggagt gagcaaaaaa
    13561 tgattgatat ttcactgata gagaagctac ttttttcaaa taagtacaca gccaaagagt
    13621 tggctacaag atggcaaata tccgaatcag ctattaaagc ctatcgtcaa aataagggaa
    13681 gcgtaggatg gcgtgattgg aaaggaatca gtattggaaa agcagaagac ataatgaaac
    13741 gtgaaggatt tcaagctgac aatgattcta aaaaagttga atagtttaat agtcgccaag
    13801 aaaagtacca ggacgagggg aaaggtagat tacaaaggaa aacactttta aataaacttc
    13861 gcaaaagcat caggttatga gtggctaggg ctatacgaat gtgatgaaaa caaagggctt
    13921 taatgtcaaa gaaaacgcaa taggggggga ataaaatttt taaaaaagat atttatgttt
    13981 ggtttaatgg aaaaaaatat atgactacta acaaaaggtg gtcagagcta gtacaaaata
    14041 cagttaaaaa agtcatgcca gatggatatc atttcaacta tatacaatgc tcacaaacaa
    14101 ctgaaagtat ttatttttca atattaaatg agaatagact ctattatttc agggtaagtt
    14161 cacaccatag gaaaaagaga gaagataatg ttacgacatt tttatcggga aaatacaatg
    14221 gcttcaaaga gttaagtgaa gcaattggtg agtatctaaa aagtaataga tttgtcagaa
    14281 tttctgacaa acatatattg atcttgttac tttatttgaa taatagcttc aaattatttt
    14341 ggagagacga cactttctac tatattgaga acggcatgga tatttattct ggtatcaacg
    14401 gcatattaaa acattatatt aaggcgttac ttcgatatgg ggttctttat caagaaaatt
    14461 taagtaacca aattaagatt agtaattctg gaaagtcatt actggcaaaa tataaaaaat
    14521 tgctttctcc tatttacaaa ggaatagata tggaagctgt aaattctgaa actttattta
    14581 atttagttgt tgatgaaaag tttgcgaatg aattttttaa aagtgaaaaa attgaaagac
    14641 taaataacct gtttcctgaa aatgcaataa aagcttatgt agataataat aaatcacgat
    14701 tatacaactg gtttattccc aaacggttgc gaggaaaaat caaaattggc gatcaagtga
    14761 aagtaattaa tctaaatcat actaagatcg ttaccgtaac ggaattgtat atagctgaaa
    14821 aagaagagat tacaaaaatg aaaccagtta ttaaaatact agataacaaa gagtgagtgg
    14881 aatatatggt agcaaaaaag aatactctaa aagaagcttc tgaaaaatat caagatatga
    14941 aaagacgagg attaatacca aaggacaaaa aaggtatatg gagagaattt gttaaggaag
    15001 tcagtagtga tcgaagtggc ggtgactttg ttggacgata tacagcaaaa ggttatagca
    15061 ggcgcttaag gatcactgtt gaatatggaa cgaatgaatc agctacagag ctgaagcaaa
    15121 ggtacttaga tacacgaaga gaagcttcaa aaaaatatga tgatttcaag agcagaggtc
    15181 taattcctaa gaagaaaaag gggatttggc gtgagttttt caaagaagaa gatagcaatc
    15241 ggaataacgg agacttcatt ggacgataca cagcaaaagg ctacactaag aatttaggga
    15301 tcaaggttga atacggaact ggtgaaagtt catgggaatt gagaaatcgc tatttaaata
    15361 cattgttaga gaaatataaa gcagaattat tggagttgtt cgatgttaat gattttatcg
    15421 agatggctga aaagtatggt catgaagaag gttttatgat caatgacatt atggttattg
    15481 ataattttac ggatttatta gcacttgcga aaaaagcaag ggatttaaga gaaacgcaga
    15541 tgtatctatt cagaaataaa aacggaagaa accggagaac tgaatcgagt gagattgaag
    15601 attactacga tgcctttaca cacgatctgg gagcctttgt agacgagata agtggtgtaa
    15661 tgaacaaata tagggtctag aataaaaaag ccggagatga gcagataaat gggaaacaat
    15721 agcatataca cacctgaaat gcagaataaa attaaaagac ttgtcggatt agggtggatg
    15781 acaagaggaa aattagattc ttttagtacg atcaaatatc ttttgatgga catttaccgt
    15841 cagaacaagc aaacaggaat acattttttg ggctatgata agccggaaga tgcacctttc
    15901 tttgttccaa gttcagccta tagagccagt acctccggta gcatgtataa cttggatttg
    15961 ttcgaggaag gagcaaagat gatcgaatat gatttcaacg aagcttattc gaggatcatg
    16021 agaaattatc ctttgccgtc aaacacttat ttgaaagtaa aatataatac agatcaagct
    16081 attttgagag cgataaatag accaaaagta agaccgtatg aaaatttaaa aacgtatatg
    16141 ttctttttgg ttaattttga aggaacagcc aaagaaggaa catatgctaa tccaaaatca
    16201 cagtcgatgt ttgcggatta tggcgaaaat cccaaaacag ttaatgagcc tatttggtta
    16261 tcggagattg agctaacact tatccttgat tattatgata ttaaagagat ggaagtccta
    16321 gatagccaca catttttgac gaaaaaagga ttactcaaag aatattttga aagaatcgag
    16381 ccattaaaaa ctgatcctga tacgttgaag ctttataaaa atcttagaac gaaagtattt
    16441 ggtgctatcg gacaaaaatc attaagagaa ggagaaacaa gcaccttcga taaatacagt
    16501 atgtataatc gagcgtattc ctccatggta gcaggcgtat ttagagatcg tatggttaga
    16561 tatgaacaga aatacgttca tagcgcttat gggctattaa aaattcgtac tgatggattg
    16621 tatttcagat ccgaagtacc agagtttgaa gagctgtata aacttggagt agttaagaaa
    16681 aaagttcata caatatccaa agaagaagtc gaaaaagctt tagaatttaa aaataaagga
    16741 tttgaaaata agggctcctt tgcgaaatct aaagatgaag ttaagggtgt tgtgatagac
    16801 gatgatttcg agtttgattt tgactaagta ctctttggaa aagatggtag gaggtaaata
    16861 tgaagaagaa aattgtggaa ggaaatgtag gtgttattat tctaaaagat tcaactgact
    16921 atgctaaatc aatggcacta aaagacgaaa aagttgcacg catggataaa gatttagttg
    16981 aatttctgat ggatgataca gaaagattaa aaggggatca gtttttgagt ttaatgaaag
    17041 acaaaggctt aaacacatat ggtttatttg attataagaa attagctata gagtgggtaa
    17101 aagaaggaga aatatttttt attgaagaat gggaaggtac tgagaactta gttcttcatc
    17161 agtatctttt gaaagcttaa tctaagtaaa acagttctga ctagttatgc ttgtcagaat
    17221 ttcaaaggat gttttccata accatcatta ttcaaaaata aaggatttga aaataagggt
    17281 tcctttggga aatctaaaga tgaagttaag agttttgtga tagacgatga tttcaagttt
    17341 gattttgact agatttcttt ttgcaacgtt taagagtaaa caaggtaaga attaagattg
    17401 tgaagattga gagtaaagaa aatagaggac gaatgtatga gaacacacta tatttattta
    17461 gaagataaaa ttgtagatga aattccggca aaaagtcgag aacaagctag aaaattattt
    17521 attgaaaaat acaaagagat caatggagat agtgaagttc cgaaagtggt cgtaacagtt
    17581 agaaaaaggc aaacaaaaaa gagaagtacg tttcatgtca gccctgatat atataatctt
    17641 atagagcgac atagaagtga agataatatt tctatctctc aatatattgc tacagttatt
    17701 gatgaattga tttcaggaaa gtatgactat gcttttgatt ttgaggatga aaaggattac
    17761 aaaagtgttg ctgtctttca atctgatttt gagaagctaa aaaactatgc tggcaggaga
    17821 aatgttaatt ttgcaagact actagaagag ttctactccg aagagttggg agtaggaaat
    17881 gaatacaaga gagaaactta tgcgaaattg tctcataagt tatcccaagc gcttaaagaa
    17941 gtgacagaag ttaaagcgga actagatgct aaacttgaag aagtgaaaaa atatcaaaca
    18001 gagaggaaga acaacatgaa gaatcaagtg gaatttgacg aatagaaaga atttgaaaaa
    18061 gcaagaaaac gctataacaa aattatgaat gaacttagct atgaacactt aacagttcat
    18121 gaataattga gtgagggtac agaaaaatat acgatcgcag atatggttaa tgaagtggaa
    18181 actcttttag cttctttctt tgaaaatggt cacgtaaacg atgaatggcg aaaagaagaa
    18241 cctaaaaggg ttaacgctta tattgctcga ttaaaaaggt ttattgataa atataaggag
    18301 aatagtgaca aattttctaa agataaataa ggagagagtg tgattacatt tgccagaaat
    18361 cgaaggaata ttttcgccaa aaataggtaa aaaacagtta gatttcagca gttttccgag
    18421 cgtaaataga gattataatc taggtgatga ggtacagata ctaaatattg atttcaaaga
    18481 actagacttc gctgttgctg tttccaacaa acccaaggca ttggaacgag tgaaaaaaat
    18541 ataccaagag aatgatcaaa atatcaaaaa tatgatcaaa aagtatagac gagataattc
    18601 aaaagttgaa gacgttttat tcaacggtac ggcaaaacat gatttgaatg tttatatggc
    18661 attaggagtg gctttgtact gtaattcaat taatcagaca gatattttgg ttaatatcgt
    18721 gaggaatagc tatcaaaaaa tattaaaaat aagtagcgaa acaaaccctt attatgcaat
    18781 gaaatatctt ggggataatt tattaaatga taaaaaattg atgcacaaca ctgaagagct
    18841 gttatctaat ttggctgcga ttaactttct attcaaaaca ggttctgaag attattttat
    18901 agataacgta ctaaaaggtg cttacggaac agcattttat ccgctttaca atatgaaaca
    18961 gcataaagcc gaacaaaagg ataacaaact tgaaattaaa aagatgaaag aactattagg
    19021 tataggattt gtaaaaccca attcaaaagt cactattgct tcgttagcaa ctactaacaa
    19081 tgatgggaaa atagtaaatg ggtttgacga ttcagatata gatatattgt caaaatcata
    19141 tttttctttg tatggagatt ttgcagattt gatgggtatt gatagttact tagtcaataa
    19201 ttttgagatc aagggtagcg agctgaatga tattgtgtat ttcgttttaa tctctctata
    19261 tgcaattgat cagaataccg agtaccgaca aattgactat ttgctagttt tttatagttt
    19321 gttatatcag ttcgttttaa ctcggttgtt taaaaatgct aaggaagaat tgatcctgaa
    19381 ttctgattat gaaatagcaa aacaaattgc aagtattaga aaagaaaaca aatctgaagt
    19441 aatcaaatta agaaaagaat taagtattgc gagtaaagaa aacggtcggt tagaatcttt
    19501 agtcaaatcg gcaaaaaaag aagaggaact tttgaagcaa gagattaaag aattaaaagg
    19561 aatcagaaaa gaacttgaac agctgaggtt tgaaaataaa gtattaaagc aattgcagga
    19621 gcaagaacat agacaatcat taaatggtaa aactgaagag ccttcactgt ctgaaatgat
    19681 agcttatcta aaacataaaa acattctttt agtgggagga catgtaaatt ttcaaaataa
    19741 attaactgaa ctattaccaa atattaaaat gtttgatgta agcgaaaata atggaaagat
    19801 acttaaaaat ttgaatacgt gtgatgcgat ttttctatat acagattact tgaatcatgg
    19861 tctatattat cgtgttatga gtgaaatttc taataaagat aagatatttt atttggataa
    19921 tcatgtgaat atgaacttaa caattaaacg tatttacgat cttttatcac ttatataaag
    19981 agagaagtga taaataggac acaaaatttg gaaagtaggt aagtcactat gagagaacta
    20041 actttatctt ctaaggaatt agaaaagaaa actgctttat taaacgaaat atttagtgct
    20101 gattgtataa tccaaagttt aaaagaaagt tcattttcaa aactaacaga tcaggaaaaa
    20161 atttcttata tgctaagttt agataatcta ggaactaaat atgatgaatc agcaagtagc
    20221 gataagcttt tgattgaatt agtatcaagt cttagtagtt ttggaaaaat gaaaagattt
    20281 aatgtgatat tagaaatttt tgcttggtta atcccttcat ttatttggtt tttttatatt
    20341 ttcatagttg aatctgagtt aatagccaag gtctctcttg gactgttaat tccgatatat
    20401 gcattttatt taataggttc aacaatttac tttaataact tgtatacacg tattcaaata
    20461 aaaaagcagt tattacatga aggattaagt ggtaatatgt taaaaagaat tgcatgggat
    20521 aaagataata aaacagatat tgagcttaaa gaatataaat acaattattt ctcgagatgt
    20581 atagatagaa agttactgtt taaaaaagaa ggattagaat ttagttttga tgaagatgaa
    20641 tttgaaaaat tagtgatgga ctggcgtaag aatttactgg atacggtaca attctaaaag
    20701 caattgaggt cgaaattccg atacttgaat catgatctat attatcgtgt tatgagtgaa
    20761 atttctaata aagataagat atttcatttg gacagtcatg tgaatatgag cttaacaatt
    20821 aaacgtattt atgatctttt atatgtttat aaacattaat gaggtgctaa aattgacaac
    20881 aataactatg acaaaaaaaa tagaagtata tgttgatgga tcacaagaag aaagggattc
    20941 cttttttgat tttttttgga aaaatagtag agaatatgta aaagcgttga atctggtata
    21001 taaacagcta atgtttgaag agttgataaa agatgcaatt aaagaaatgg atcaaggtta
    21061 tgctgagaga gaagaaagta ttcaaaatcg gattgaaaag ttagcatata ctgatccaat
    21121 agatcaaaaa aaacttgata aagcaaaagc tgaattatct aaattgagaa atgaaaaaaa
    21181 taaagaagcg agagaaatat taacgcaagc gatagggttg aagagtcaaa cgagaacacg
    21241 agatgtagca aaaaaattag actttgactt tggagatttt gttgattccg caaatatgaa
    21301 agctagtaaa gattttggca atgattttat cggaattgtg accggacaaa gaacgcctag
    21361 aatatataaa aatagaaaca aatatggatt attacctttt agaggtcgag atattaaaat
    21421 ccataaagat gataagtttt atatttattt acctaaaggc tttaggttta aagtgaattt
    21481 aggtagcaag ccaagaaaag caagagatgt tgcttcagtt ttacaacaca ttatggatgg
    21541 cgattacaaa ttaaatcaaa gtaatcttat ttttgatgga agaaaattat tttttaatct
    21601 aacaatttct tttgaaaaaa gctataatga aatggaattc aagaaaggta atgaattgat
    21661 cgtaaacttt gcagatttgt gtagcggtga atgtttaatt aatgataaat ctgtaatgtc
    21721 ttttggtgat agccaatata taagaaactt caaaagtaaa attttagcaa tgaaatcaaa
    21781 agaacaaagc cgaggagtct tttctaaggg cggtcatggt cgaaaaagaa aattagatca
    21841 agatcgatgg gaagcaataa agaaaagaga aagtaatttt gttaaaactt ataataatca
    21901 atgttctgca aaaatcgtta aaactgctat tcgcaataaa gctcaaatta ttcgtatcat
    21961 tgaaccagat aaggcttatg gcgaatgggc ttactatcaa ttgaaagaac aactagtaaa
    22021 caaaggaaaa agatatggga tagaagtcag aagttagtaa attaaacaat cgttgatttg
    22081 gcaacgaaat agtgagggaa aatccactca ctgaagggta aactccgtca agttgtgtga
    22141 ccagacggaa ggacgaatgt tgttgaggtt ttagaaccat ataaaacagc atgtttctca
    22201 aatttttagg ataagggaaa ttgctagcag tttgttttag aaccatataa aacagcatgt
    22261 ttctcaaatg agtactttaa attaattgat ggcgttcctg ctttagaacc atataaaaca
    22321 acatggattt aaaacaagaa gtaagtcagt tctcctttta ctttagtaag gaaaaataaa
    22381 gtattctcaa taaaacttaa aataagcaaa cctttgtttt aatagctaat cgaagtaaaa
    22441 cccaaagcca tatgaacccc tgaatcagcg aaagtttatg atgatttgga ttactaatag
    22501 tttaggaatt agatggtaaa tctaaattaa acaatcgttg atttggcaac gaaatagtga
    22561 gggaaaatcc actcactgaa gggtaaactc cgtcaagtta tgtgaccaga cggaaggacg
    22621 aatgttgttg aggttttaga accatataaa acagcatagt tctcaaattg tttaaaatga
    22681 ttgtcaagtt aaaaaaatct gggatcatat aaaatagtat agttctcaaa tatgattaga
    22741 aaccctatta aagagcaata ttaagccaac ttacttaaat cgtttaatta agaacaataa
    22801 tctcttttag tcagactttt agtctgactt ttttgtattt ccaaaattta ttctttacaa
    22861 aaacgaacat acgtttgtac aattacttgt gaatggggga ataaaaatgt ttgattacag
    22921 tagagaaccg gtgaatgact attttttaat cgacatgaag tctttctatg ctagcgtgga
    22981 atgtattgaa cgaaatttag acccaatgac tacaccgtta attgtcatga gtagatcgga
    23041 caacactggt tcaggtctga ctctagcttc ttcacctact gcaaagaaaa aatatcatat
    23101 tactaatgtt agtagagcta ctgatttacc ttatccatta ccaaaagaac ttcatattgt
    23161 tccaccacga atgaacttat atataaaaaa aaatatggaa gttaatcaga tttttagaaa
    23221 gtatgtcgca gatgaagatt tattgattta ttcgattgat gaatcaattt taaaagtgac
    23281 aaaatcctta aatttattta ctagttcagg aacaagaagc gagagaagaa gagaattagc
    23341 taaaataatc aaagatgaga ttcagaaaga aacaggtcta atagcaacag tcggaatagg
    23401 agataaccca ttattagcta aacttgcact ggacaatgaa gcgaagagta gtaaagaatt
    23461 tatagctgag tggacttatg agactgtacc tgaaaaaata tggaaaataa acgagatgac
    23521 tgacttttgg ggcattggat cacgcatgaa aaaaagattg aatcatatgg gaattatgag
    23581 tatagaagat ttagcaaatt ggaatcccta cacaattaaa agtcagttag gtgtaatcgg
    23641 actccagtta tatttccata ccaatggaat tgatcgtacg gatattgcta ttccaccaga
    23701 accaaccaaa gaaaagtctt atgggaattc acaagttcta ccaaaagatt atacgaggag
    23761 agaagaagta gaactagtag tcaaggagat ggcagagcaa gtagccattc gaattagaca
    23821 acacaattgc aaaactggtt gtgtacgctt agcaattggt acgtcaattt tggaagaaaa
    23881 atcaggtttc tcgcaccaga tgaaaatacc aattacagat aatacgaaag aattacaaaa
    23941 ttattgtctt tttttattca ataagtttta tgaggggcag gaagtacgac atataggagt
    24001 aacttatagc agattagtat acacagattc cctacaatta gatttattta gtgatccaag
    24061 aaaaaagatt gctgaagaaa atttggatag gattattgat aagattagac aaaaatatgg
    24121 atttacagct attatacatg cttcatctat gttagaaggt gcaagaagta ttacgagaag
    24181 cactttaata ggaggacacg caggaggatt aggaggtttg cataatgatt gaatataacg
    24241 atagaggacg tttaaaatgg tcgggatttt atttgtctga tcatactgaa agaatcaatg
    24301 cagacaatgt acaacgtgct aatcataatt tggcaaaaga acaaatgacg actgaagaga
    24361 ttgcgaaaat actgagaact gctgtcataa aaaatagacc cgtctgcatt caaaaagagg
    24421 aacgaaacgc tgaaggttac tatccagatg atgtagtagg aaaaattatt ggatatgacg
    24481 agcttggtgt atttgtggaa gaagaaaaag ttcactatga tgaaattagg aatgtttctt
    24541 tctcggaatt attaaaatgg agtcagatat gaacattatt aaaaaatacg aattcggtta
    24601 tattacgtat gaacagctaa tagaggaatt atggggatat ggtcaacaat taattaatga
    24661 agtggggatt gattgctttt gtttctatat tgaatccgga gcaggatacc ataagaacag
    24721 atactatata tttccatatc caccaaaata gtctcttaga tatttttaaa ttttctccaa
    24781 atactttcaa ttattgtgaa atatttccct ttaattaatc aaatttttta taaaagtaat
    24841 atataaaata aattgcttga agtattatat ttagatatca taatttatgg aagaaaaatt
    24901 aattaaaaag gatgagatta taatggggtt actggttccg agagctaata cttatactat
    24961 acctttaacg aaacgccaat taattgctaa aagatatcaa aggattacta gagccatcaa
    25021 tagagagttt tggaattctg aaagtgatac tgctcacagt ttatacgtag gttcttatgg
    25081 gagaggaacg gcaattagta ctagtgatat agatataata gttgaattac cgatggcaga
    25141 atttgacaga ttcaaaaatt atttatcaaa tggcccgtct aagttattac aagttataaa
    25201 aaacgcattt caggaaatac taccaaatag tgatattaga gctgatggac aagttgttaa
    25261 aataaatttc catgatggaa tcaaatttga aatagttcca gcttttaatg aaaaagatta
    25321 ttggggtgag tcaaaagggt ttatttatcc agattcaaac atgggaggga actggaaagc
    25381 tacaaatccc aaaaaagagc aagaagccat gaaattaaag aatacaaaaa gtaataattt
    25441 attatatgct acgtgtaaac attttcgaca tgtacgagat acagaattta cgagttatca
    25501 tctttcagga atagtaattg acagtttcgt atatgaggct atgggaaatt ggaaattcgt
    25561 tgaaaataac agtgggggtc aaaacattag ttctgtatct tatgagacag ctttattaga
    25621 atactataac tcacataaag ttatgggcgg tttaaattta tactctcctg gaagcaatca
    25681 atttgttaat tctgatagta gtatcatatg tttagaaaaa gtattaaaaa aaatagcttt
    25741 ataaagtttt atggaggaac aacatgggag acgaaaaaaa gcgcttggaa gttcaattac
    25801 gtgaagcata tggacgagta acgtatactt acaccactca tttaaagcaa gccgataata
    25861 ttatttctga caataaaatt ttaaggtgtc ttcaaattgg tttgtcagcc cttactactg
    25921 gaggggtact tagtaccatt attactgata atagtgagtt aacctttgtt gctggattat
    25981 gttcaacaat ttctcttgga ttaaacttat actttaagaa ttttgatctt gctgaagaag
    26041 ctaaaaatca tcaaattgtt gccgataaat tatggttgat cagagaagaa tacgtaagct
    26101 tactaacaga tttaaatcgt ttaagtgttg aagatattgt gaaaaaaaga gatcaattaa
    26161 tgaacgaaac tcatgaagca tataaaaatg cccctaaaac aaatagtaaa agctatgaat
    26221 cagctcagaa agcattaaaa tctgaagaag agcaattttt tacagaagag gaattggata
    26281 agatgttacc agagcatctt agaaaaaaga gttctaaata ttgaactctt tttttatgct
    26341 taaaattctc ttgtaatttt tctattcatc tatatttaag aagaaatatg atgatagaaa
    26401 catctcctaa agccaccgca agccgttcta agcatagctt agcccgacat tgctagatat
    26461 gctcatctca ccatgaacgt ttcctaaagc caccgcaagc cgttctaagc atagcttagc
    26521 ccgacattgc tagatatgct catctcacca tgaacgtttc ctaaagccac cgcaagccgt
    26581 cctagccatt gcctagtccg acatcgctag attagtgcat ctcgccataa aagttttcta
    26641 aagccaccgc aagccgttct aagcatagct tagcccgaca ttgctagata tgctcatctc
    26701 accatgaacg tttcctaaag ccaccgcaaa ccgtcctagc catcgcctag tccgattttg
    26761 cttgatcggt tccatttctg tttatttagt gggtcaacac cgtaatctta aatagccatc
    26821 gcctatttaa gattactagg tctgctttga aggtaaaaag tctttcattc ttcaagactt
    26881 ttaggctttc aagctacgcc tgaaagcatt gaaattcatc gctctttatt tggttttgcc
    26941 atttataatt ccttacgccc attatctttt tcgcctgtca taccttgtca gaagtgttta
    27001 gagcttatct catctttgga ttaatacaag tggcgtaaat ttttttctaa acgcaagcgt
    27061 tacgaaaata atttacttaa acacttgtat taatccggca gataagaacg cattctaatc
    27121 actcccgaca aggcaagaca ggcgaaaaag ataaataaat gaacgaaagg aagaaacata
    27181 aaatgcaaaa ccaaatagcg atgaagaaaa aaccacctcc ctctaaaaca atccaaacaa
    27241 aaaagaattt tgaaaaacca aaaatcaaaa acagaaagaa gcgttcaaca tgagagatag
    27301 aattttagaa gttaatcaag aaatggtaaa caagttaaca aatatcagtg ggaactatga
    27361 aaagtacgat ggtactttac ttgataacta tatttttcac gatacacaaa atatcagaat
    27421 tggcagaagt aaaccgagag cgttcattct catcaaagag aaatatttga atgaatggaa
    27481 tagcacctat gaaatggtta tgactgataa ctttgaaaag gtattggatt tcagaaatgc
    27541 tttgttgggt tctgatatgg gaaatagcag tcttgaatct attgaagttg aaaacaagac
    27601 cgagaatgaa ggtgaaggat tgattaagaa tgtcttgaaa atcgaacaaa aatatatcag
    27661 tgaagataga agtttttata gagtcacaag ctcggttggt ctagcaaact ttgctactaa
    27721 cttaattgga aacaatgcta gtgaatcttt gctagtgatg tgtctgaata caaagaatga
    27781 ggtcattgct tatagcgaag ttttcaaagg tggaattaat caatcagtag cgataccaag
    27841 agagattttt caactagctt tgctaaacaa ttcagcaaaa atcgcagtat cacataatca
    27901 tccgagcgga tatgaatttc caagtgaaaa tgacaaaagt tttactacga atttgaaaaa
    27961 cgcaggaaaa acaattggta ttgaattgtt agaccatatc atcgtaacaa gtaaaaacac
    28021 aagctattac tcatttcaag aaaatggttc tttataaaaa ttagagaaaa gaggaaaaca
    28081 agatgggtga aagaacacaa gcgttaatta cagtcaaata tggtaacagc aaaacgaaag
    28141 caagcgtgtc aattcattat cagtggggat atggtcgata catgttgatg gacgttttga
    28201 atcaaggaat atatttaatc aataaagcat atgacttacg agatgaatca agagaagaat
    28261 tagcaaaagg aatctttgat agaagtacag gagttatcaa ttttgtcacc tatgaatctg
    28321 gagtgagtgc ttatgatgaa acagggtttg gtgggcgcta tgaaccttat caagaaacga
    28381 ttgaagatgt gatgaagttt tcagataaca atgatggcta tgctcattta gagatggtgt
    28441 tcgatgaaaa tggatttagt ctagatgata aaaccagatt gcgattgttt gattttgatg
    28501 gtcaacagat aggactaaaa aagttttgtg aaagcggaaa agagtttgct acgaaagatt
    28561 ttattaaggg atacagatta ttgctagaaa attatggaat aaacctagaa gaagagagcg
    28621 tgaaataagt gtttgaaaaa agaaaagacc agttccctaa tttagaagcg aaaagtcaaa
    28681 aagtgattga tggtgaaatg attgaattat tccaaaaaag ttatggaacc atagaaacta
    28741 ccgcaagttg taaatactac tttcaaggaa agagaaacac gtttaaaggc tttgccgatt
    28801 ctgatgaaga attggcaaag aaaatcattg attggcttgt cagcagtgaa cacgtagcaa
    28861 gagaagatac cgttagaaca acgattaatg gggaagtcct aacaggtact agatgtgttt
    28921 gttgtaatca tgaatttgta gaagatgaag gttgctaccg aatcaatgaa tatggtgatt
    28981 ttatttgcca gagttgccac gagatagaat ttccaaccga aagaagttgg tttgaataca
    29041 tgctacaaga ttatgagatg gaaaaagaaa atggtgaagt agttttttgt accaaagagg
    29101 aattgagtaa gagaagcgat gatgaaattg aagattggtg tgtgtggtta gtaggggcag
    29161 atgattgcgt tgcatattac acaacagcag aataggagtg tgaatcagca tggaaggaat
    29221 ggattttcta gatcatgaag atttggttga ttttggttat acatggaaag gaatggtagg
    29281 tattagcaga agtcttgcta atgcctttta cgaaagaaat tatgcagtgt atgttcttta
    29341 tgatgacgat acagaaagcc ttgttgacga ggaatataaa ttggatttag agaatgtttt
    29401 atatggaata gaaaaggaag atttagcaaa atatattttt tcatggttgg gtcaataagt
    29461 gaaaggaaga tagaaacatg acaaaattta aaagtaaggg tatgcaatgg tgttcttatt
    29521 attatgttgg gaatgatgaa cagcttttga gaaaagccca acaaaaaggt tatccagata
    29581 agatgttaaa acagattggc tattacacgc cagaagagag gatagaaaaa gaatcttgtg
    29641 aggtactttt tgatagtgaa cagctattag ttttgttaac tcaaaataaa ttaatttgtc
    29701 aatttgagaa taattaatat ggggtgaata accatgaatg agaataagat ggtattaagt
    29761 gaatacgcaa gattgagaac cattaattgg ttgaatggta tgcaaagtag tggcgctttt
    29821 gatggatttg atgaagagaa tatggacttt gaaagtttat cgaatgagga tattagatat
    29881 tattttatcg aatggtattt tacagaacca gatgaagagg tggaagatga acaagaatta
    29941 attttagaac gtcaaagtat tttatgcagt gaacaagata gtaagaggtt gtatggttta
    30001 tatgacttgc aactgaatgg attaaaagcg cagtacgttg ctgatagttt cgatgatatg
    30061 gtaggacagg gggcagatag ggttgtcact tattcagaag atgatgaaga ggaatgggaa
    30121 aactattcag atgatgagat atgctttaat gatgggtata tgcctattga aatcaccgaa
    30181 gaagaatatg ataaagtgtc cgatagtgat gagattggta tgtgtacggt tgtaagtgga
    30241 ttggatatag atgtccaaag agtagaggtt gcataccaga agatgaaaga acgtataaaa
    30301 agtatagtag aatcttgatt taattggatt gtttgcaaag tatctaatta agagtataat
    30361 ataatcataa atgtagttaa tggatacaat aatgagtata ttatacttaa aagaggtgaa
    30421 cgaaatgaag gtaaaaaatc caactgaaaa aagcagaatc caagtcggaa tagacaagaa
    30481 tttaaaagag aacgcagaaa tgatattgga agagctaggt ttaaatccta caacagcaat
    30541 cacaatcctt tataagcaag tggtcgctag aggtgagttt ccagtagaga taaagctaag
    30601 tgaagaggaa aagcaaggaa tcagattgca acagctaact aaggatatgc cagttgatgt
    30661 tctagataca gatgaaaagt tagaagagtg gttcaatgaa gcctaatgag atagtaacgg
    30721 tctatgttgc atttacagat aaaaatggtg gtaaaagaag acctattttg gtggttagtg
    30781 ataaagaaga tagagtagaa ttttttggaa tcacaagtca atatgagaag aaatcagacc
    30841 aaataaagag agtgtatttt cctattgcag aatgggaaaa agcaggactt aaaaagcaat
    30901 catgggttga tgtagggtct ttaaaggcta ttcctaaaag ccgagagaac atatcattta
    30961 aaaaggttgg tatgctatca gctagtgata cgaaaaagct aaataggttt atcgagagat
    31021 tacaaagaaa agtctaacaa ggcttttctt tttttgatta tatgtatatt aag
//
LOCUS       DAHHPA010000023        30007 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.024, whole genome shotgun sequence.
ACCESSION   DAHHPA010000023 DAHHPA010000000
VERSION     DAHHPA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.024"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            318..1187
                     /locus_tag="NBH55_000974"
     CDS             318..1187
                     /locus_tag="NBH55_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCD1508975.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1396..2421
                     /locus_tag="NBH55_000975"
     CDS             1396..2421
                     /locus_tag="NBH55_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCD1508976.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2460..3410
                     /gene="manA"
                     /locus_tag="NBH55_000976"
     CDS             2460..3410
                     /gene="manA"
                     /locus_tag="NBH55_000976"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCD1508977.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3504..5207
                     /locus_tag="NBH55_000977"
     CDS             3504..5207
                     /locus_tag="NBH55_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1508978.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5209..7242
                     /locus_tag="NBH55_000978"
     CDS             5209..7242
                     /locus_tag="NBH55_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1508979.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7253..7693
                     /locus_tag="NBH55_000979"
     CDS             7253..7693
                     /locus_tag="NBH55_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1508980.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7893..9020)
                     /locus_tag="NBH55_000980"
     CDS             complement(7893..9020)
                     /locus_tag="NBH55_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1508981.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9023..9700)
                     /locus_tag="NBH55_000981"
     CDS             complement(9023..9700)
                     /locus_tag="NBH55_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1508982.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9964..11730
                     /locus_tag="NBH55_000982"
     CDS             9964..11730
                     /locus_tag="NBH55_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCD1508983.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11745..12182
                     /locus_tag="NBH55_000983"
     CDS             11745..12182
                     /locus_tag="NBH55_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1508984.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12195..13346
                     /locus_tag="NBH55_000984"
     CDS             12195..13346
                     /locus_tag="NBH55_000984"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD1508985.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13408..14106)
                     /locus_tag="NBH55_000985"
     CDS             complement(13408..14106)
                     /locus_tag="NBH55_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCD1508986.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14099..15451)
                     /locus_tag="NBH55_000986"
     CDS             complement(14099..15451)
                     /locus_tag="NBH55_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCD1508987.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15674..17074)
                     /locus_tag="NBH55_000987"
     CDS             complement(15674..17074)
                     /locus_tag="NBH55_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCD1508988.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17235..17972
                     /locus_tag="NBH55_000988"
     CDS             17235..17972
                     /locus_tag="NBH55_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCD1508989.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18097..18576
                     /locus_tag="NBH55_000989"
     CDS             18097..18576
                     /locus_tag="NBH55_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCD1508990.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18573..19340
                     /locus_tag="NBH55_000990"
     CDS             18573..19340
                     /locus_tag="NBH55_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508991.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19570..20139
                     /gene="lepB"
                     /locus_tag="NBH55_000991"
     CDS             19570..20139
                     /gene="lepB"
                     /locus_tag="NBH55_000991"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD1508992.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20243..20845)
                     /locus_tag="NBH55_000992"
     CDS             complement(20243..20845)
                     /locus_tag="NBH55_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCD1508993.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20951..21420
                     /locus_tag="NBH55_000993"
                     /pseudo
     CDS             20951..21420
                     /locus_tag="NBH55_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21526..22110
                     /locus_tag="NBH55_000994"
     CDS             21526..22110
                     /locus_tag="NBH55_000994"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCD1508994.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22113..23186
                     /gene="prfA"
                     /locus_tag="NBH55_000995"
     CDS             22113..23186
                     /gene="prfA"
                     /locus_tag="NBH55_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCD1508995.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23179..24018
                     /gene="prmC"
                     /locus_tag="NBH55_000996"
     CDS             23179..24018
                     /gene="prmC"
                     /locus_tag="NBH55_000996"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCD1508996.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            24011..24190
                     /locus_tag="NBH55_000997"
     CDS             24011..24190
                     /locus_tag="NBH55_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1508997.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24212..25237
                     /locus_tag="NBH55_000998"
     CDS             24212..25237
                     /locus_tag="NBH55_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCD1508998.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25412..26656
                     /locus_tag="NBH55_000999"
     CDS             25412..26656
                     /locus_tag="NBH55_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCD1508999.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26809..27756
                     /locus_tag="NBH55_001000"
     CDS             26809..27756
                     /locus_tag="NBH55_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1509000.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27890..28519
                     /gene="upp"
                     /locus_tag="NBH55_001001"
     CDS             27890..28519
                     /gene="upp"
                     /locus_tag="NBH55_001001"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCD1509001.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28658..28900)
                     /locus_tag="NBH55_001002"
     CDS             complement(28658..28900)
                     /locus_tag="NBH55_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509002.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29021..29093
                     /locus_tag="NBH55_001003"
     tRNA            29021..29093
                     /locus_tag="NBH55_001003"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29054..29056,aa:Thr,seq:cgt)
     gene            complement(29212..29850)
                     /locus_tag="NBH55_001004"
     CDS             complement(29212..29850)
                     /locus_tag="NBH55_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1509003.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10201 a         5292 c         6091 g         8423 t
ORIGIN      
        1 aaatttttct tactactcga aactaagcga ctttttcaca acctcttcct taaacggtgt
       61 tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaataggag gagctatttt
      121 tgtttggctg ttcttattac ttgatgcaga acagcttttt cacaacctct ttttataaga
      181 gtttttactc attaatttac aaaaaacaag caaaatgcga attttctcac atttttttta
      241 acaaaatcaa agttattgct tgaaagaaga agaatccatc gttaaaataa agcttgacgt
      301 agaggggagc aagaaatatg aaagattttc aattgggggc tgccacctat ttaaaaagtg
      361 tagccattcg ggtgaaagac cgagataaaa tgattgattt ttacaagaac gttgttggct
      421 ttgatttgaa aagagaagaa aacgaattag ccattttagg aacagaagaa gctggaagtg
      481 aaatgttgct gctggaagaa agtcctagag cgaatgatca catgggggtg atcaaaaagc
      541 tgaaccgttt ttcattgatt atccctagca cagaagaaat ggcagatatc ctttgtcgta
      601 tccgtaaaat gaattatcca atcaaagaag ctttagaaga tcatggacgt atggggattt
      661 tgctcgtaga cccagaagag aatgaattgg aaatttacca tgaagagaat aagaacgacc
      721 aaagagaaat tccagaaaca atggatcaag aagcattatt aagaaaatct gaagaaaaat
      781 tcagtaaact atcaacaggt tcttattttg aaaaaatcca tttaaatgtt actaacttaa
      841 caaaagaacg ccaatttctt aatcatgtac ttggtttgaa ggtacataat gaagaagagg
      901 gcatccatgt attgaatgaa ggagatttcc atgttggttt gacagaagct agcggaggcg
      961 caattgattt gcctacagat gaagtgattg gacttgattt tctgaaattc atgatcgata
     1021 ctgaaagcat ggaaggactg attgctcact tgcagcaact atcagttgat ttcttcttag
     1081 acaaaaagca cacagtactc acaatctatg atgctatagg aatcgaatgg tggttcgtca
     1141 gtgagccaaa agaaaagaat aaaactgaca ttcgatgcga cgcttaactt tttctttctt
     1201 aacgattgtc gcatgtctcg tcattttatg aacggtcata ctatcgctta ttctagagat
     1261 agaaagagaa aatagtatac cgggggaaac tgcgcggttt ttcgcgcagt ttttttgtgt
     1321 aaaaaaggtt gattcctcat agattaagct ttaagcttgg actgtaagaa tctgatagaa
     1381 aagcaggaga aaaagatgta tccacacacg aaacagctta ttttgaaaaa gatggaagaa
     1441 ggattttatc caggtgtagc agtcgccttc ataaaagaaa atcaagtaga aacatttaca
     1501 gccggctttg cccaagtcgt tcctgaaaag cagctgatga cagaagatct gttgtttgat
     1561 gcagcctcac tgacgaaagt cgttgcaacc acttcattag ttttacgtct attagaagat
     1621 ggaaagatag aactcgatca accgcttcat aaatatcttc ctgaatttga aaatcagaca
     1681 atcactttgc gtcatttact gacacatact tctgatatca aaacatggat accaaacaga
     1741 gataagctat ctcaggaaga attgatcgcc gcttatttga agctagaacc aggtgagcaa
     1801 ctaggaaaag aagtaaaata tacagatgcc ggtacgattc ttcttgggtt gatgctagag
     1861 gaaatttacc aagaaactgc agtggaaatc tttcaagaac aagtattgga accattagga
     1921 atgatgaaca gcttatttct tccttttcct tccaaaaaaa tcgttcccac agaacaactg
     1981 ccttcgggtg aggttttgcg tggacaaact catgatccga aagcaagagt tttggctgaa
     2041 catgcaggta acgcgggtct ctttattgat ttgaaagaca gcattacttt tgtccaaatg
     2101 tatttgaaca agggaaaaat ccgtaatggt cgttttttag aagaagagac gattgattct
     2161 ttactgcaag atcaaacacc gaaaaaaaat ggctatcgtt ctttaggatg ggacttaaaa
     2221 aaggatgtag aagacggtca tgcgattctt tttcatactg gttatacagg tacgtttttc
     2281 ttgattgata taaaaacaca agaagcgttg atatttttgt caaatcgcgt acatcctgtc
     2341 gatcatcgtg cggagtatct catatgtcga gatgaaattg ttgaaaactt tttgaaagag
     2401 aaagcggtat taccgaaata atgctataat agctatgaaa aatgagagga gtttgaatta
     2461 tgcaggaacc tatgttttta aaaccagttt ttcaagaaaa aatttgggga ggaagtcgtt
     2521 tacgttcagt ctttggtttt gatatcccta acgataaaat cggagaagat tgggcgatca
     2581 gtgcgcatcc tcatggtgtc agtgtagtag aaaatggcga attcaaaggg aaaagattag
     2641 atgaactatg gtcagaacac aaggaattgt ttggccatcc ttcagaacca gtgtttccat
     2701 tattgatcaa gatcttagac gcagaagatg atctatctgt acaagtacat ccagatgatg
     2761 cctatgggat gaaacatgaa ggtgaattag gaaaaactga atgctggtat atcatcgatg
     2821 ctgaacctgg tgcagaaatc atctatggtc atcatgcaaa gacaagagaa gaactagctg
     2881 agatgatcaa agatgggcgc tgggatgacc ttttgaagaa agtaccagtt aaaaaaggtg
     2941 atttctttta tgtaccaagc ggaacgatcc atgctattgg taaaggaatc atgattttag
     3001 aaacacagca aagttctgac acgacttacc gcgtgtatga ctacgacaga aaagatgcgc
     3061 aaggacaaac aagagagctg catatccaac aatcgatcga tgtaacaact gtccctgcga
     3121 aaacaccaga attacaaatc aaagaagtcc gaaaagggaa ttcatcgatc gtcacttatt
     3181 tagaaactga gtttttcaat gtatatgagt gggatattaa aggaatcaca tctttcaaaa
     3241 aacaagcccc ttatacattg atgacggtaa tcgaaggagc aggagacctt gtcgttgacg
     3301 gaaaaacata tccattagaa aaaggaacaa gctgtattat tccaaatggc gtttctgagt
     3361 gggcggtcca aggtgaacta gcaatcattg cgtctgaacc aggcaaatag ttcccagata
     3421 gctgaaagca gcagacggtt tttcgtttgc tgctttttct acataaaatc cgtccatcaa
     3481 taaatagaag tctggtgaaa aagatggaat acaaatggaa agcaaacaac agcagtcgcg
     3541 tgtctatgaa acaactgaga aaagctggac atcacttaaa tgaaatgatc atgccaaatc
     3601 taagtctgtt tatcgcttgg ggaatcttgt cattagcaac aaaaggtgtg tctggcgatt
     3661 tgcacaccac attagaagag gtatgccatt ggatgatcca gctgctttta cccatcttga
     3721 tcagttattc aggcgggaaa tcggtagggg gacaaaaagg ggcagtcgcc ggtgcaattg
     3781 cttctttagg gttgatcgct cgttccaatg ctccacaaat cgtaggagcg atgattatcg
     3841 ggccgcttgc cggcatcgta tatgaacaat ttgtttctag attccatgtg aaattcaagg
     3901 ctggatatga aatgcttgtc agcaacctac tagtaggact gactggtagc gttatttgta
     3961 taattggaat cgtttttatt gaaccagttt taaatagtgt tcacttgttg ctcgcctccg
     4021 ttgtgtcttg gctagtcaca atgaatctgt taccacttgt cagtctgatc ttagaacctc
     4081 taaaagtact atttttcaat aatgcaatca atcatggtgt attgacacct ttaggaatcg
     4141 aattctcaca aacagtcggg tactcttcct tatttttgat ggaggcaaat cctggacctg
     4201 gtttagggat cttactttct atcctttgtt ttgcagaaaa acaggaaaaa gtaaatgcga
     4261 gtggagcatt gatgatccat gcgattggcg ggattcacga aatctatttt ccatttgtct
     4321 tgctgcgacc gtatctattt ttagctgtaa tggctggtgg ggcaagcggc acgttgatat
     4381 ttcaatggtt agatacggga ttagctgcac ctgtatcgcc tgggtcacta ctcgtgattt
     4441 ttgccaatgc gccttatcca caactagctg gggtaagtat agggatagca atcagtacat
     4501 ttgtgacctt catttgtgca gcgatactat tgaaattacc aaataaagaa aaagagaaca
     4561 aaacggtcaa agaaagggga gaaatcttgg aaccatctat cattcattcc gtcatttttg
     4621 cttgcgactc aggtatggga tcaagtgcta tgggagccgc cctgctgaaa aagaaattag
     4681 cagcagaaaa aatcgatctt tctgtcggat acagctctat ctatgaattg aataaccaag
     4741 taaatcagct ggttatcgtg caacatgaac tagcgggtct tgcaagagaa aaagtacctc
     4801 aaagtcaaat ctatgtactg gatcactttt tggaaacaga tatcttgctg tcacgaataa
     4861 aagaagcgat ccaatttcca aagcgggaag aaaaaaaaga gatagcagat atcacagaac
     4921 aaaaaacgtc gcctgctaat attgtatttt tgtataaaaa caacagacgc ggctcacaaa
     4981 ctatgggcat gacgatttta agtcaattag caaaaaaagc gaaaaaacag tttgttatct
     5041 caaaagaatc aatagaaaaa atcaaaatcc aagaagaaac gatttatatc gcacaaagag
     5101 aactagctgt aactgaacag ctagaagaaa agatccctcg gctagttcta gttgaacatt
     5161 gggtagctac gaatgaatac gaaaaactcg tgaagggaga aatataagat gtatttatcg
     5221 cccagagaaa aacaattatt agcagaattt ctcagtagcc cgtcacctgt atctatccaa
     5281 aaaatgatga atttattgaa ggtcagtaaa cggactattt accgcgaatt agataatctt
     5341 gaagtctcat tgaaatcggt aaatgcttct ttgaaaaaag cagctagagg ctcatttgtc
     5401 attgaggcat cagaagaagt attagagcaa ttaaaagaaa atacagatgg gaaaagtaat
     5461 gacttatcca ctgctaaacg acaacacgcg attttagccg agttgttact gacgacagag
     5521 ccgttgagtc ttgctcattt catggaatca tatcgcatca gtaataccac tttttatgca
     5581 gatatcaaac agttggaaga aagtattcgc caactgcctt tggaaatcat tcggaaccag
     5641 ggatacgaga tcgaaggacc ggaaaaatac cgccggcttc tgacagcgaa tgtattagaa
     5701 ttagaaatca atgaatatga actttttcat agtatttctt tcgatgcatc actgaattat
     5761 ttttttcgat tcgttgatcc gcaacatcta tctcttgcac gcaaagtaat cggggaagaa
     5821 ttgatccaaa aaaagaccaa tctaagtgat agaaagcttg aacatttggt tttgatgctg
     5881 acattgacga tggagcgtgt gacgaaaaat catttactga caaatgagac atataatagt
     5941 ctggcaaaca aagaattact acagacagct aaacgattgt tttcacggat cgcttctgag
     6001 acaaaacaac tttatccagt aaatgagatc gtcttttttg caggattatt aaatgacttc
     6061 acgaactcat ttgatcagaa tttctttgaa gagacgttcg atacacagct tgcttattta
     6121 gtaaaacaac tgattcaaga agtaagtgaa gagacgaatg tccattttta tgaagatgaa
     6181 actttgtaca aaatgctgtt gactcattta tctggtgtgt tttcaagagc cgttttacaa
     6241 gaagagacac tgaccaatcc gattttagaa aaaatcatgg ttcaatacga ggaagttgcg
     6301 aatgcaatcc gtaaagcagt agagcggatt tttcaagaaa aaaaattatc cgaagaagag
     6361 atcgcttata tggtccttca ttttgcgaat tcattggaac gaagtccgaa agaaagcaat
     6421 atcagtattg caggattttc gccaagtgga ttagcttcta ctagcatgtt ggaactacga
     6481 ttgaaaaggt attttccctt catccaacaa attcatttct atcggattgc tgatttaaaa
     6541 aaaatcgatt tacaagaaga atatgatgtt gttatttcta catctatttt accaggatac
     6601 ggtggaaaat atattttagt ctccccgcta ctgctagaag atgaagtcaa gcagctaaaa
     6661 gaagcttttt cgcatattga gaagaaaaaa aggcaaaaaa ttacgtataa cagcaataac
     6721 gagtggatga tagaagatac ttatgaggaa acaatgcatt tcatggatca aatcaatcaa
     6781 ttactcggtc gtttttttat ccaagaactg gataatcctg agacgatttc cgaaacgatt
     6841 cgcagcttat ttccttattt tcctgaagga ctagtaacgg atgaagaggt ggttcatcgc
     6901 cggctgctta agcgatatga acaagcacct atcggtattc ctcatacaaa tatggggtta
     6961 ttccatacat ccagctcatt gatttctaaa ccgatctttt gtattttcaa tttgaaacat
     7021 ccgttgacca ttgaagggat ggatagacag ccagtcgatt tgtcccgtat gttagttatg
     7081 ctggcgcctt cgccaatcca agaaaaagac gggattcttt taggaaagat cagtggagca
     7141 atcatcatga atgacttgaa caccgagatc ttccgctcag gaaataaaga aatcgtttat
     7201 cagttgttag cgaaaatcct gattgaagaa ataaagaaat gaaggtgaaa agatgataca
     7261 gctaccagca gaaaaaatat tgctcgatca atcctatcaa aattggaaag aagcgttgac
     7321 cgagatcggc cagtggatga cccaacaagg gggaatcaaa ccggcatata tccaatcaat
     7381 gatcgaaaga caagaaaaag cgagtgtcta tatcgggaat ttcgtagcct tgccacatgg
     7441 gactgacaaa gatgaagaag tagtagaaga aggaatctat ttagtccaag tgcctgatgg
     7501 tgtggacttt ggaacaaaag aggaaccaaa aatcgcgaca ctcttatttg tagtggcttt
     7561 aaaaaaagaa catcagttag cggtgctcca agatttagcc tttttctgtt cagacatcga
     7621 ccaagtaatg gcgctcagtg atgcggaaac cattgaagaa gtccaaatga tttttgaaca
     7681 tggacatata taaccaatgt tcacagtaaa aaaagctgcc ttgcatcaag taataacttc
     7741 aaccaagata aaaataagag gagctatttt tgttgggagg ttcttattac tgcggatgca
     7801 agcttttgaa aactatttat tctagaaaac cgtttatttg aaatagggga cggtgacaag
     7861 gtttttgtca ccgcccctat tatttttgtg ttttaatagc tcttttttat atagatagtg
     7921 cccataccta tgaataataa aacgatactc catataccaa gtgtcaagat accattcatg
     7981 aaagtaacct gttctgaagc tagtggcgag aaaatatctc ctcctatgat cacattttga
     8041 aaatcaacat aagaagttgg caagtaatga ctgattgatt ctatccatcc aatcgaagaa
     8101 aacaagcgat actctgctaa gaaaagacaa agaagtgtac ctaaaagatt gactagagaa
     8161 tgttcaaata aagcagatag aagaaaactg attgtactta aaaagagcac ccacaacaac
     8221 aggaacagaa agctctggaa aagatactca tacatcggca ggattcgtat ctcctgacca
     8281 atgatcgttc ctacaggata acggaagtct cccaaaccat ttttgattat tactagtata
     8341 gagacgatca aaaatggcat cacgagactt agcatagctg caccataata aatggtaaaa
     8401 cggatgctat gtttctttac catactatcc ggtaatagat tgactagaga gattacctgc
     8461 tttctttttt cataagagat gatggaagaa agaaacaagg cggttataca taaaatcaaa
     8521 aaactagaaa tcattcctga aaaaatattt tcgtagtaat ttgctaaaga gagatgcgct
     8581 ggcaagtcaa aaatagaata tctttggatc tgatgttctt tcatataacg gagcaactcg
     8641 acacgttttt gttgttcaat gattggaatt ccttgtcttt gaccagaaat cagtcgatcc
     8701 agtatatctt tttcataatg ataagttgct tcgaccattt gctggacatt ccctgtgttt
     8761 tttgcatgta agattgcttc gatcagtttg ttgcttttct ctaaactttc cacaaattct
     8821 ttctcattct catcttcttt caacgtttca atcaaacgtt gcatctcttc atagttatga
     8881 ttcagttcat cgaattctgt ataagcaagt ttatgatcaa aaaaaagtgg gaatgcaaag
     8941 agcacgacca acaatagagg aaataaaaag tttactttgt tttttagtaa gagattgcgt
     9001 tcaaatgtaa gtaaagacat agttacacct catataattt acgataaata gctaaaatct
     9061 cttccatatt tgaggattct gtgacgattt ctccattttt tagaaaacat gctttagaac
     9121 aaatcgattg gatattcgcg atagcgtggg aagaaagaag aatcgtcttc ccctctttct
     9181 gccattgtct gatgaggcta ttcaagatat caatacttcc tgggtctaat ccgttcaacg
     9241 gttcatcgaa taacatgaca gatgaatcac tgatcaagta agcagcaagc aagacatgtt
     9301 gtttcattcc tagagacatt tttccaatcg gcaaagtaag gtactcagtc atcttcaatc
     9361 gttggaaaac agtttggcat tctactttgc tctgccaata gtatttgatc aaagtcaaat
     9421 aatcaacagc cgatagatag tcggataact gattcgttga ttctagaaaa aagaaagacc
     9481 ggcgataatc tgtataagca gtaggagaga tgttgtgaag ggacaattga tcataagttg
     9541 ttggcagcag ttgggcaatg ccattcagca aagttgtttt gccaaatccg ttcggggcaa
     9601 tcaaagcagc tacttctcct gattctacta aaagattcaa ttgtcgaatc acacgttttt
     9661 taccacgttt catgttcatg tttttgatca gtaatgtcat agataggcct ccaatataaa
     9721 atttaataac ataatagaat ggaaaacatc taaattaaat gctaacaaaa gttattgttt
     9781 gcatattata aaaaaattaa aataacagtt ttatttaaaa ttgaagtttt ctgataccga
     9841 aatcttttta ttttgctgac atactcaaac ctttggcaca aagtttcgtg ccaaagtacg
     9901 actatttctc tttttagtaa gcggatacaa ttagagtatc aaaaggagag agggagagac
     9961 aaaatggaac ggacgattcc caaaaacaac atttcgctaa aagcgagggt acaaaaatta
    10021 ggcagtactt tatcaagcat ggtcatgcca aatattggag cactgattgc ctggggtgta
    10081 ttaacggctc tatttatccc agatggttat ttaccaaatg aggctttcgc taccatggta
    10141 agcccaatgc taacttatct gattccttta ttgatcggtt atactggcgg aaaagtaatt
    10201 gctggtgacc gcggatcagt tgttggggca attgcaacga tgggtgtgat tgtcggaaca
    10261 gatattccaa tgatgctagg cgcgatgatc atgggacctc ttggtggata tgccatcaag
    10321 aaatttgatc agctattcca aaaacggatc aaatccggtt ttgaaatgct agtcaataat
    10381 ttttcagcag gtcttatcgg ttttggatta gcgttattag gtttttctgc gattggtcca
    10441 gttgtcgatg cattgactca agcaatggca aaaggtgtag agatcatttt atctgcccat
    10501 ttgattccat tgacaagtat ttttattgaa ccagcaaaaa tcttattttt gaataatgca
    10561 atcaatcatg ggattttgac accattggga actgaacaag tagcagaggc tggaaaatct
    10621 attctattct tgcttgaagc aaatcctggt ccaggacttg gcgtattact tgcttttact
    10681 ttgtttggca aaggagcagc aaaatcttca gcaccaggag ccatgatcat tcacttttta
    10741 ggcgggatcc atgaaatcta ttttccttac gttatgatga aaccgttatt attcttatca
    10801 gttataggcg gaggaatgtc aggaagtttc attttccaat tgatggatgc tggactaaga
    10861 gcgccagctt ctccagggtc gatcattgcg attctagcga tgacaccgtt caattcttac
    10921 cttcctgtta ttttgggcgt gattgttgcc gcagctgttt cgtttggtat ttctgcagta
    10981 attttgaaag cggatgcgaa agatacaaat gaagacttcg aaaaatctgt tcaagagaca
    11041 aaggcagcta agcaagcttc caaaggtgcc caaggtattc ctcaaggagc tacattctct
    11101 ggcgtacaaa aaatcatttt tgcttgtgat gcaggaatgg gatcaagtgc gatgggcgct
    11161 tcgatcttga gaaaaaaagt acaggaagca gacttgcctc aaacggtgac caatcaagca
    11221 atcagccaac tgacagatga ctcgaataca ttgatcgtta ctcaggtcga actacaagaa
    11281 agagcaaaac aaaaagcacc gagcgcgcgt tttgtctcag tagaaaactt tttgaattca
    11341 cctcgatatg atgaaatcat tcaagaatta agcagcaagc agaaaacatc agtttctgaa
    11401 attgagaatg agccagcaga agagtttctt tcagcaaaca tcaaacaaat caaagagatt
    11461 ctacttgtcc acgatgatcg ttccggatca gcaacaatgg ggatgaccgt gctcaaagat
    11521 atattagaca agaaccattt aactatcccg ttaagaaaag tcaatatcca agatttaaaa
    11581 gatgaacctg attcactgat cgtagccaaa gaaagtttag cggaaaaagt acagatggct
    11641 gcacctcaat ccattcattt atcagtcaac agcatggtca atccgcaaaa atacgaatcg
    11701 attgcacaat atttgaaaca gacagcttaa gggaggaaaa cagcatgcaa agtttgccat
    11761 tcgacatgat cgagctcaat cgatctttta aaaccaaaga agaagcgatc cgctattgcg
    11821 gtcagatgct tgtagaagca gggtgcgttg atgaggaata tatcgatgcc atggtagata
    11881 gagacaaaat gttatctgtt tatatgggga attttatcgc gattcctcat ggaacagatg
    11941 aagcaaaaaa atatgtgaaa aaatccggca tctgtgtcgt tcaagtacca gatggtgtag
    12001 attttggtac cgaagaagaa gaaaagattg cgactgtttt gttcggtatt gcaggagtcg
    12061 gagatgagca tttgcaactt gtacagcaaa tcgcacttta ttgcagtgat atggacaatg
    12121 ttgtccagct agcagacgcg ctcagcaaag aagaagtaac ggaaaatcta gcaattgctt
    12181 aaggagagag aaaaatgaga gcagtacatt ttggtgcagg aaatatcggt cgaggattta
    12241 ttggagaggt tttggcagct aatggatttg agattacttt cgtagatgtg aatcacacga
    12301 tcattgatgc tttaaatgaa agaaaagaat acgaaatcga attagcagat gaatcaaaag
    12361 aaaaaatcac tgtaaaaaat gtcaaaggaa tcaacaatca aacaaatcca gaaaaagtaa
    12421 tccaagaaat cgctgaagca gatttagtta ctaccgccat tggcccgaat atcttacctt
    12481 ttattgctga gttgattgca aaaggtatcc aagaaagaga aaaggaagga agcacaacat
    12541 ctttagatat cattgcctgt gaaaatatga ttggcggttc agcatttcta gaaaaagaag
    12601 tgtataaata tcttccggaa acatcattta ctgacaaata tatcggcttt ccaaatgcag
    12661 cagtcgatcg aatcgtccca ttacaacacc atgaagatcc tttattcgtc caagtggaac
    12721 ctttcaaaga atgggtcatt gacgatagcc aaagaaaaaa taaaaaaatc caattaaaag
    12781 gggttttata tgtagatgat ctagaaccat atatcgaacg caaactattt agtgtcaata
    12841 ctggtcatgc gactgtcgct tatacaggtg ccttgttagg ttaccagacc attgatgaag
    12901 caatgcaaga tgcgttagtt gtggcacaat taaaatcagt tttgcaggaa acaggaagtt
    12961 tgctgattgc caaatggggg tttgatgcag agcagcatca tgcgtatatt gaaaaaatca
    13021 ttcatcgctt ccaaaacaaa aatatctctg atgcgatcac acgtgtcgct cggacaccat
    13081 tacgtaagtt aggttatcaa gaaagattta cacgtccagt gagagaacta caagaacata
    13141 acttgacttg cccacatttg acggcaacaa tggggattat ttttaattac tatgatcctg
    13201 aagatgaaca aagccgtcaa ttgcatgaaa tgaagatcca tgaaaacttg gaacaattga
    13261 ttcaagaagt cacaggaatc aatgatccga aaacaatcgg gaatatcaag caaaacgtga
    13321 accgttatgc caagcaagtc gcttaaaata ggatacgaaa aaggggaatg tgacgaaatt
    13381 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
    13441 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
    13501 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
    13561 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
    13621 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
    13681 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
    13741 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
    13801 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
    13861 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
    13921 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
    13981 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
    14041 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
    14101 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
    14161 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
    14221 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
    14281 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
    14341 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
    14401 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
    14461 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
    14521 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
    14581 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
    14641 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
    14701 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
    14761 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
    14821 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
    14881 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
    14941 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
    15001 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
    15061 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
    15121 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
    15181 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
    15241 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
    15301 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
    15361 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
    15421 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
    15481 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
    15541 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
    15601 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
    15661 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
    15721 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
    15781 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
    15841 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
    15901 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
    15961 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
    16021 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
    16081 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
    16141 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
    16201 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
    16261 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
    16321 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
    16381 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
    16441 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
    16501 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
    16561 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
    16621 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
    16681 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
    16741 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
    16801 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
    16861 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
    16921 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
    16981 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
    17041 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
    17101 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
    17161 acagctttcg attaaaatag aaatggctta taataaggca aatcctagaa aaaagaagga
    17221 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
    17281 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
    17341 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
    17401 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
    17461 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
    17521 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
    17581 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
    17641 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
    17701 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
    17761 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
    17821 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
    17881 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
    17941 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
    18001 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
    18061 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
    18121 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
    18181 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
    18241 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
    18301 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
    18361 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
    18421 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
    18481 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
    18541 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
    18601 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
    18661 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
    18721 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
    18781 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
    18841 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
    18901 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
    18961 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
    19021 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
    19081 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
    19141 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
    19201 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
    19261 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
    19321 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
    19381 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
    19441 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
    19501 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
    19561 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
    19621 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
    19681 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
    19741 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
    19801 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
    19861 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
    19921 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
    19981 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
    20041 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
    20101 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
    20161 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
    20221 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
    20281 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
    20341 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
    20401 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
    20461 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
    20521 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
    20581 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
    20641 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
    20701 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
    20761 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
    20821 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
    20881 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
    20941 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
    21001 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
    21061 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
    21121 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
    21181 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
    21241 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
    21301 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
    21361 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
    21421 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
    21481 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
    21541 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
    21601 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
    21661 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
    21721 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
    21781 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
    21841 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
    21901 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
    21961 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
    22021 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
    22081 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
    22141 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
    22201 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
    22261 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
    22321 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
    22381 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
    22441 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
    22501 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
    22561 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
    22621 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
    22681 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
    22741 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
    22801 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
    22861 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
    22921 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
    22981 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
    23041 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
    23101 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
    23161 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
    23221 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
    23281 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
    23341 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    23401 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    23461 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    23521 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    23581 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    23641 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    23701 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    23761 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    23821 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    23881 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    23941 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    24001 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    24061 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    24121 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    24181 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    24241 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    24301 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    24361 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    24421 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    24481 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    24541 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    24601 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    24661 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    24721 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    24781 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    24841 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    24901 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    24961 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    25021 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    25081 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    25141 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    25201 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    25261 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    25321 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    25381 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    25441 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    25501 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    25561 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    25621 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    25681 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    25741 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    25801 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    25861 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    25921 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    25981 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    26041 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    26101 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    26161 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    26221 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    26281 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    26341 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    26401 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    26461 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    26521 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    26581 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    26641 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    26701 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    26761 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    26821 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    26881 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    26941 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    27001 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    27061 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    27121 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    27181 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    27241 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    27301 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    27361 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    27421 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    27481 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    27541 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    27601 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    27661 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    27721 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    27781 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    27841 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    27901 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    27961 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    28021 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    28081 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    28141 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    28201 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    28261 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    28321 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    28381 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    28441 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    28501 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    28561 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    28621 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    28681 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    28741 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    28801 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    28861 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    28921 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    28981 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    29041 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    29101 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    29161 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    29221 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    29281 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    29341 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    29401 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    29461 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    29521 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    29581 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    29641 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    29701 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    29761 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    29821 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    29881 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    29941 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    30001 ctagcaa
//
LOCUS       DAHHPA010000024        29670 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.025, whole genome shotgun sequence.
ACCESSION   DAHHPA010000024 DAHHPA010000000
VERSION     DAHHPA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.025"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..1571)
                     /locus_tag="NBH55_001005"
     CDS             complement(<1..1571)
                     /locus_tag="NBH55_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCD1509004.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDR"
     gene            complement(1804..2568)
                     /locus_tag="NBH55_001006"
     CDS             complement(1804..2568)
                     /locus_tag="NBH55_001006"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCD1509005.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(2569..3516)
                     /gene="prmA"
                     /locus_tag="NBH55_001007"
     CDS             complement(2569..3516)
                     /gene="prmA"
                     /locus_tag="NBH55_001007"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCD1509006.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(3529..4017)
                     /locus_tag="NBH55_001008"
     CDS             complement(3529..4017)
                     /locus_tag="NBH55_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCD1509007.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(3983..4654)
                     /locus_tag="NBH55_001009"
     CDS             complement(3983..4654)
                     /locus_tag="NBH55_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCD1509008.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            4792..6078
                     /locus_tag="NBH55_001010"
     CDS             4792..6078
                     /locus_tag="NBH55_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCD1509009.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            6150..6344
                     /gene="ssrS"
                     /locus_tag="NBH55_001011"
     ncRNA           6150..6344
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="NBH55_001011"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            6422..6706
                     /locus_tag="NBH55_001012"
     CDS             6422..6706
                     /locus_tag="NBH55_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509010.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            6779..7258
                     /locus_tag="NBH55_001013"
     CDS             6779..7258
                     /locus_tag="NBH55_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD1509011.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            7481..7624
                     /locus_tag="NBH55_001014"
     CDS             7481..7624
                     /locus_tag="NBH55_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCD1509012.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            8202..9785
                     /locus_tag="NBH55_001015"
     CDS             8202..9785
                     /locus_tag="NBH55_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD1509013.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(10222..11406)
                     /locus_tag="NBH55_001016"
     CDS             complement(10222..11406)
                     /locus_tag="NBH55_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCD1509014.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(11431..12438)
                     /locus_tag="NBH55_001017"
     CDS             complement(11431..12438)
                     /locus_tag="NBH55_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1509015.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(12610..12969)
                     /locus_tag="NBH55_001018"
     CDS             complement(12610..12969)
                     /locus_tag="NBH55_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509016.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(12941..13312)
                     /locus_tag="NBH55_001019"
     CDS             complement(12941..13312)
                     /locus_tag="NBH55_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCD1509017.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(13329..13631)
                     /locus_tag="NBH55_001020"
     CDS             complement(13329..13631)
                     /locus_tag="NBH55_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509018.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(13648..14118)
                     /locus_tag="NBH55_001021"
     CDS             complement(13648..14118)
                     /locus_tag="NBH55_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCD1509019.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(14093..14371)
                     /locus_tag="NBH55_001022"
     CDS             complement(14093..14371)
                     /locus_tag="NBH55_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCD1509020.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(14368..15417)
                     /locus_tag="NBH55_001023"
     CDS             complement(14368..15417)
                     /locus_tag="NBH55_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCD1509021.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(15374..16303)
                     /gene="tadA"
                     /locus_tag="NBH55_001024"
     CDS             complement(15374..16303)
                     /gene="tadA"
                     /locus_tag="NBH55_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCD1509022.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            16435..18189
                     /gene="ade"
                     /locus_tag="NBH55_001025"
     CDS             16435..18189
                     /gene="ade"
                     /locus_tag="NBH55_001025"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCD1509023.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(18287..18949)
                     /gene="trmB"
                     /locus_tag="NBH55_001026"
     CDS             complement(18287..18949)
                     /gene="trmB"
                     /locus_tag="NBH55_001026"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCD1509024.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(19019..19801)
                     /locus_tag="NBH55_001027"
     CDS             complement(19019..19801)
                     /locus_tag="NBH55_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCD1509025.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(20005..21216)
                     /locus_tag="NBH55_001028"
     CDS             complement(20005..21216)
                     /locus_tag="NBH55_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509026.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(21209..21946)
                     /locus_tag="NBH55_001029"
     CDS             complement(21209..21946)
                     /locus_tag="NBH55_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509027.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            22084..22518
                     /locus_tag="NBH55_001030"
     CDS             22084..22518
                     /locus_tag="NBH55_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCD1509028.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            22511..22855
                     /locus_tag="NBH55_001031"
     CDS             22511..22855
                     /locus_tag="NBH55_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1509029.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            23035..24045
                     /locus_tag="NBH55_001032"
     CDS             23035..24045
                     /locus_tag="NBH55_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD1509030.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(24114..25058)
                     /locus_tag="NBH55_001033"
     CDS             complement(24114..25058)
                     /locus_tag="NBH55_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1509031.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(25042..27762)
                     /locus_tag="NBH55_001034"
     CDS             complement(25042..27762)
                     /locus_tag="NBH55_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1509032.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(27759..28979)
                     /locus_tag="NBH55_001035"
     CDS             complement(27759..28979)
                     /locus_tag="NBH55_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCD1509033.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(29260..>29670)
                     /locus_tag="NBH55_001036"
     CDS             complement(29260..>29670)
                     /locus_tag="NBH55_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HCD1509034.1"
                     /translation="SIPYNNIEEIIEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNV
                     SVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFA
                     MGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         8861 a         6097 c         5196 g         9516 t
ORIGIN      
        1 cgatcgagtg cttcttgcag tttgttcttc gtcaaaatat ctttaggcgt aaatcctaga
       61 tctgtaatac atttgaccac ggattcgtgg cctttgatca cattttcttc tcgatcttgg
      121 gctttgaaga aacgtttgat cttatttctt gctttgctag tagcaactaa tttcaaccag
      181 tcgcgacttg ggccaaatga atttggagaa gtcatgatct caatgatatc tccgtttttc
      241 aatttgtaat caagctgcac cattttgcca tttacttttg caccagtggt tttgttaccg
      301 atatctgtat ggatactgta tgcaaagtcc aatggtccgg atccttttgg taactcagta
      361 acgtctcctt ttggtgtgaa gacgtagact ttatcactaa agatatctcc ttttacgcct
      421 tccataaatt cagaagcatc atagctttcg tcttgaagtt cgagtatctc atggaaccag
      481 cttaattgtt tcgtcatacc atctggttct actttttcat tttttccttc tttataggcc
      541 cagtgtgcag caaccccgaa ttcagcgatt tcatgcattt cctgtgttcg gatttgaatt
      601 tctaccggat ttcctgccgg accgatcacg gtagtatgca aagattggta catattagct
      661 tttggcatgg caatataatc cttaaatctg ccgggcattg gtttccattt tgtgtggatt
      721 gctcctaata cagcataaca atccttgatt gaatctacga tcactcgaat agctagcaag
      781 tcataaattt cgttgaactg ctttttctga tctttcattt tacgatagat tgaataaatg
      841 tgttttggcc gtccatagat ttctgcaaaa atccccagct cttctgtcgc tattcgaata
      901 tcttcaacag taccacttac gtatttttca cgttcttctc tcttcgtctg catcaagtgg
      961 acgatacggt aatactgttt tggatttaga taacgaagtg ctgtatcttc taattcccat
     1021 ttgatccggc tgatccctag acgatgtgca agaggcgcat agatttctaa agtttcctga
     1081 gcgatccttc tttgcttatc ttctcgcagg tgcttcaacg tacgcatgtt atgtagtcta
     1141 tcagctaatt tgaccatgat aactcgtaaa tcttgagcca ttgctagcag catctttcgg
     1201 tggttttctg ctagctgctc ttcgtgggat ttatatttta ttttccctaa tttggttacg
     1261 ccgtccacta acatagcaac gtcatcgcca aattcttctc tcagatcttc taaagtgatt
     1321 tctgtatctt caacaacatc atgtaagaag cctgtagcca ctgtatgggg atccattttt
     1381 aaatctgcta atatacctgc gacttggata ggatgaataa tgtagggttc gcctgatttt
     1441 ctaaactgtc cgtcatgtgc agcagtcgcg tattcgcatg ctttctcaac aaatgctaca
     1501 tgttcgggac ccatgtattg actaacgagc tttatgacac cgggtcccgt taaaatttct
     1561 tcttttgtca caggaatcga tccccctttc ttttttcggt aaaacgggaa tcgggacaaa
     1621 tatctgtttg ataattgtcc caaaatccgt gcagttatac gtttttttac ggaaatgttc
     1681 gctttttaaa agctttttca aacgattttt cgtatccatt gtataaataa ttgtttctat
     1741 tatacaaatt gttgactatt ttttcaattc tttctgtcga tactctttta ttaatgaaga
     1801 acgttagctg aaagaaggat tcattaattc ccattgatag ctagcagcac ttaataaata
     1861 aagaggtgct gtttctgtac gaaggattct tggtcctaat ccacaaagta tccctttgtg
     1921 ttctacaaaa taatcgactt catcgcggga taatccacct tctggtccaa agacagctaa
     1981 gattcgacta tctggttttg tttcactaag aattttggct gcatttgatt tttcgccttg
     2041 ctttgctgat tcctcataag caaccaagac cacgtcataa tcatctagcc gctcaaataa
     2101 atcttgtttc gtttccaata actctactga tggcacgatc tgccgatggg attgttccgc
     2161 tgcctcttga gcaatctttt caagccgttg ttgtcgtttc atacgttttt ttgcatccca
     2221 tttaacgaca gaggctgatg ccgggaaacc aagaaaagct tgcgctccta attcagtgga
     2281 tttctgaacg atccaatcca gcttttcccc tttaggatag ccactcgcta tggtaatcga
     2341 acttggcagt tccttttgat tttcttcttt tttaatttct ttcagataaa cagcctcttc
     2401 agtaatctcc gtgatttctg caacaatggc tatttgatca ttgaatgcca aaaaaacttc
     2461 atcctcgggt tccatcctca taacccgtac aatgtggtga tagttttccc cttctaaaac
     2521 aaaatagtct tttgattcat atgattcttt taaaaaatac cgctgcatct attccacctc
     2581 tggttttttc aaaataattg cataccagtc tttttgctgg aaaatttgat ccacgataaa
     2641 cccttgttct aacatggcat ccagaaccat ttgcttcttc tcttcaatga ttcctgaaac
     2701 gatcagcgtg cctgtttgct tcagtaaacg ccaagcatct gggatcatca ataagatgat
     2761 atctgctaaa atgttcgcaa cgattacatc actttcaatc tcgattcctg tcaaaagatc
     2821 gttagctgat acatggacat ctttggctat ttcattcata tccatatttt ctttcgccga
     2881 acgaacggct acttcgtcta aatcaaaggc atagacatct tttgcaccaa gatattttgc
     2941 tgcaatactt agcacgcccg acccagttcc cacatctagc aatgtttcac caccacgcaa
     3001 gactgtttct agtgctgtca atgtcaagga agtcgtggga tgagtgcctg tgccaaatgc
     3061 cataccagga tctagtgtga taattttttc tagaggatct tctgtctggt agttttccca
     3121 gcttggcgca accgtcagca ttcgcgtaat acgcaccgga tgatagtatt tcttccaggc
     3181 tgttgcccag ttgctttcct ctacttcact aactgttact ttattttttc caatagctag
     3241 accaaactca ggtaattgtg tgatacgatc tttgataaaa ggcaagattt ccggtaagaa
     3301 agtcgtttcc ggaaaataag ccatgactag tgcgccttct ttcagcgatg taaaatcatt
     3361 tttatctaaa atctcaccat attgatccga ctgaaaatgt tcaacatcta aagaatcttc
     3421 aatcgcgaca ccactcgccc ctgcctccat caaaatatta gcaactgctt cgactgcttc
     3481 actttcagtt tctactttta cttcattcca tttcatatct gtcactcctt aataacttgg
     3541 gtacggtaaa taagtatctg cttcattttg ctgttttact tctactaatt ctgctagttg
     3601 ctgattatcc cagtgaagct cgaatacagc ttcgtcatcg tcttcatcca aaaaatcaag
     3661 caaatcgctt tgcccttgag ctaatacttc atttagatat tccactaaag aatcagctac
     3721 tgattttgcc aacccttttt ttccttcata agggattact gctagataat tgcgttcatc
     3781 aaaaacagat ttctgaggat taaaaagaag tacgccgtct tcaaattcga taattggttc
     3841 ttgcgaaacg atcccttctg catcatcaat ctcaaattgt gctttatttt ctgcaaacaa
     3901 gcgaacaacg atctcgatcg tatgattttt tgtatcccaa tctaaagcta catcatagtc
     3961 tgtcacttgt ttttcaatta tttcatctaa atatgttaat agatccttct ttttcatttt
     4021 ctttcctcca accccaatct ttctcgattt ggttttttct ttgtaaagag atataaggat
     4081 ttcccgacac aacaaaacgc aacggctttt ctgtccatac acctttgttt gggataccga
     4141 ttctaggcaa agcattgatc ttttttggtg tctttttttt ctcaaaaact aagcgtaacg
     4201 aactatctgc tatagactga ccataaagtt cttgaggaat tgctaatgct tccactaatt
     4261 ttcccggacc gttgctgata tctggaccag tttttccgcc acgattttta ctcatttgat
     4321 caatcattgc ggcgggttcg attgcacgta tcattactcc ttgaggaatc ccttctggct
     4381 gagtaatgat gttcaaaatc cgatgagtat gcatcgtgta aaggtagatc gtacctggtt
     4441 tttcatacat tgcccgcaca cgcggtgttc ttcttaaacc atagctatgg gcggcctcgt
     4501 cttctggacc taggtatgct tccgcgtcta caatatatcc agctaatctt ccttctggtg
     4561 tgtcatgctc caaatacatc cccaataaat cccgtgccac ttctggcgtc gttttattcc
     4621 aaaaaaattc atctatcttt tgtttatccg tcatttttct cacctacttt atcatacaca
     4681 atattagttg gataagctat gaaaacaaaa caacagaagg ctgttctttc taggcatctt
     4741 gctaaatatg atataattaa gggacgtaaa aacaagaagg aggaaaatcc tatgcaacag
     4801 ccattggctt atcgcatgag acctcgaacg atcgatgaag tcgttggtca gcagcatcta
     4861 gtcggggaag gaaaaatcat acgccgcatg gtcgatgctc gcatgctctc ttccatgatt
     4921 ctatatggac ctcctggtac tggaaaaacg agtatcgcaa gtgcgatcgc cggctctact
     4981 aattatgcat ttcgcatgct aaacgccgct acagattcaa aaaaggatct acaagttgta
     5041 gcggaagaag caaaaatgag tggtacagtg atcctcttat tggatgaagt acaccgatta
     5101 gataaaacaa aacaggattt tctgcttcct catttagaaa gtggccgaat catcttgatc
     5161 ggagcgacta ctgagaatcc ttatattacc atcaatccag cgatccgcag ccgtacacag
     5221 atctttgaag tcaaaccatt aacagaacaa gacatccagt tggcagtcga acatgcttta
     5281 agggataaag aacgcggcct tggacaacaa gcaatccagt tagacgaaga tgctcttctt
     5341 cacctttcgc gtgcaacgaa cggcgatttg cggagtgcat tgaatgggct ggaactggct
     5401 acactatcga ctccttcaga taaagaagga aggattcatc ttacactgtc gatcattgaa
     5461 gaatgtgtcc aaagaaaagc tcttacccat gacaaaaatg gagatgctca ttatgacgtt
     5521 atttcggctt ttcaaaaatc gattcgtgga agtgatgtgg atgctgcact tcattatctc
     5581 gcccgtttag ttgaggcagg tgatctggca agtatttgcc ggcgtctgat ggttatcggt
     5641 tatgaagata taggtttagg taatcctgct gctgcggctc gtacagtgaa cgctgtatta
     5701 gctgctgaaa aattgggatt gcctgaggca cgtatcccac tagcagacgt cgtcgtcgat
     5761 ctttgcttgt cgccaaaatc caattctgcc tacatggcat tagatgcagc acttgctgat
     5821 attcgagagg gcaaagctgg agatgttcct gatcatttga gagatagcca ttacaaaggt
     5881 gcgaaatcac tcaaccgtgg cgtgggttat cagtatcctc atcattttga tcaggcttgg
     5941 gtcaatcagc aatatttacc agataaactg aagaatgctc agtattatca gccaaaagat
     6001 acagggaaat atgaacaggc tctgggtcaa cagtattacc gtataaaaga gtggaaaaag
     6061 catcctccaa aaaactagac aagaaaacgg ttctgtttct gcgagctctc tcattgctct
     6121 ttttaaattc atatgctact atagaggtgg gaaatctgtg atgttcgaat tgtcctcttt
     6181 gtgtgttgac cgaacatttt tattgatatc ttgggacccg tcttgtgtca ctgctggtaa
     6241 catgccttat catttggtag ttcgaagcta cgaccatggg acccacctgc ttgaattcgc
     6301 gggatcaata ctacaggaca aacaacggca ttaacggaga ttaccatctt ttaaagaata
     6361 aagcaatttg ctcttggcaa attgcttttt ctatttgttg cttattttgg aggatttgtt
     6421 catgattagg attttaatga ttatcgccac tttagtatta ttattcgtta gctattattt
     6481 atttaataaa caggatatct tttttgtatt gatcaacaaa aatgataaaa atcaaggatt
     6541 cctacaattt tacggagctg catatgctgt tttaggcgta atgggtattt taacagcttt
     6601 tttcaatcaa cgctttattg ccttggtctt tttactgatc gtaattattg tttctgccac
     6661 cttcagtatc cgatttgcaa aaaaaattgc tgaaccaaag cagtaaataa ttctcttttt
     6721 ccctaaagtg ttctacaata aaagagtaca atacgattga cttgaggagt gaatgcttat
     6781 gttacaacaa tacaaaaaaa tcatggttgc cgtagatggt tctgatgaag ctgaattagc
     6841 ttttaaaaag gcggtcaatg tagctatccg aaataacgga gaacttctat tagcacatgt
     6901 cattgacaca cgttctttcc aaaccgtttc atcctttgat ggaatgcttg cagaacaagc
     6961 tactgaaatg gcaaagcaaa cattggcaga ttatgaaagc aatgcgaaga aagctggctt
     7021 gaataatgta acctctgtcg tagaatatgg ctcaccaaaa caaattattg ccagagaaat
     7081 cccagaagat aatcaagtag atttgattat gttaggagcc acaggattga atgctgtaga
     7141 acgtttattt attggttctg tttctgaata tgttattaga aacgcagctt gcgatgtgct
     7201 tgttgtccga actgatttag aaaaccaatt gccagctaaa gaccacacca aagcttaaca
     7261 cgtagacgac ctgccaacta tcggcaggtt tttttgcaca aaaaaccgtg ataaaaacaa
     7321 aagacgcggc aagcactcat cggtctttat aatgtttaaa ataaagggaa aacagtcaat
     7381 gagttcaagt aaaataaaaa aaagtgaaca ccagggcgat tagtcgccct ggtgtggttt
     7441 aaaacgacct aagcatgcca aagcttattg gttcgtcaat gtggaagttt ccgatgaatc
     7501 tgatacgaaa aaatatgaag tatcgatggc ggataccgat tttatcgcca aactaaaatt
     7561 ttatttcggg ttcgggtacc gcgagaaggc aagtgctaca ttcggtaaat cattcaagaa
     7621 ttgatgaaag gctaccgctt gccattgcaa ccacaacgat actcctgaac gcgatgttgg
     7681 tgagatttcc aaagagaaga gctgtttaat gcaacggtat taagcaaatg gcaaaaggaa
     7741 gtcatgtaga caaagttttt tatcaaacac catatgattt cgcctgaacg ctggtttagc
     7801 tcagaataca atgatgtggt ccacgagaca ttaccctcag tcagataaaa aaagctctct
     7861 gacagaatta cacatcgata aatattaagt aaatgtcagc gctagttgaa ctttctgaca
     7921 ctttcaggta cactggaaaa gacaactaca cgaaggaggg aaaatactat gatgcgcatg
     7981 actaaaataa caagtaatga aaattaccaa cgataccata tcgcatttag ccatgcaatt
     8041 ggttttacag ttgctgttga cgggacaagt ttgtccaaaa cagttgaatt cacgtaaaat
     8101 caatggccat ctcaaggtat ttttctcatg ataatctgag tttaaataac acaggccatg
     8161 gataaccatg gcctttttga gtggtctcaa ggagagagat catgatcata caagcaattt
     8221 ctattaaaaa aacaatcggc gggaatccat tatttgagga tttatcctgt caaattgaag
     8281 ctggtgaaaa aattgcctta gtcggtatca acggaactgg caaatcaacc tttttacaaa
     8341 tcttgaccgg cagagaaggt gtcgacagcg gtgttatcag tcgcaaaaaa ggcttaaaga
     8401 tcgggacggt cgaacaagaa cttacagttt ccgaagcgac cgtcaaccac tacttgctcc
     8461 attctgccgc tgagatacag gacctcaaac agcaaatgag tcgctacgaa gcattgatga
     8521 cagaaccaga tacaaactta gagaagtgtc tggcccgtta cggtgagctg cagcatcgct
     8581 ttgaagaact aggcggttac gtattagaag accggattgc agcgatcttg caaggcttag
     8641 ggattccaca ccgtcaggaa gcacggttaa cagaacttag cggaggcgaa cgagttaaag
     8701 tcgctttagc caaaatttta gcagccgatg ccgatttact tttactcgat gaaccgacca
     8761 atcatttaga tcttaacagt attcgctggt tggaaaacta tttacagaat acaaaaaaat
     8821 cggttctggt cgtttctcat gatcggcaat ttctcgatca agtcaccaca aaaacatggg
     8881 aactggaaga aggtgcattg attgagtatc ccggaaacta cagccgtttt cgagtcttga
     8941 aagaagctcg cttggctgag ctgacaaaaa attacgaact gcagcaaaaa gaagtccagc
     9001 ggttgaaagt catgattcgt cgttttcgtc agtgggcaca tgaaggagac aatgagtcct
     9061 tcttcaaaaa agccaaagaa ctggaacgcc gcctggcgaa actgacactc gtcaaaccgc
     9121 cacctccccc gaaaaatcgg ctgcaaagct tgtctaatgg tggaaaatct ggcaaagaag
     9181 ttttcatcat tcaaaacttg caccagcagt acgctgatca agtactattc aaagacagca
     9241 gttttgcagt ctatcgtgga gatcatttag ccatcatcgg cgacaatggt gcaggaaaat
     9301 caaccttact caaattatta ctcggtgagg aaaagcctta cggtggcacc atcaaattag
     9361 gtagtagctt gcaaatcggt tatttgccgc agcagctgca gtttgccgat cccgatagcc
     9421 gtttgctggc ttacgccatc acattgactg gaaacgaaga agtcgctcga cggcaattag
     9481 ccaaaagtgg cttttatcaa agcgatgtgg gtaaacggat caaagattta tccggtggtg
     9541 aaaaaatccg tttggcgttg ctgaaactct ttttagaaaa aatcaacgtc ctgattttgg
     9601 atgaaccaac caatcacctc gacagctatg ctcgagaaga aattgaagaa atgctgcaag
     9661 actatcaagg aacgctccta gcagtcagcc atgaccgcta ctttttgcag caacacttcc
     9721 aagaagcact cgttattgat caggagcaga tcagccgcca gccgctcggc caactgattg
     9781 actaggacgt tttttcgcaa caaaaaactt gattttcata cctgtgcttt cacgattcag
     9841 gcaaagtaca ggtggaaaat caagttttta ttattcgatc aatgaatgtt tcggatcggt
     9901 tttctagcta aacataaagc gaagaataaa gtcgacagcc cacgaggctg atctaaaaat
     9961 gttttttctt tttacatatg taatgtccct tataaagatg aatgaaaata aaatattggg
    10021 attttcctga aaatcaataa ataaaatgcc agtcttttcc cttttgacca cttttccaag
    10081 caaaattccc taagaagatc ctgtaaaaaa gaagggaaat tgaagttaaa ttaccatttt
    10141 gcggtcttct ccgcaaaaaa aacacatgca agaattccct cttgcatgcg tttcatcttt
    10201 tatcctaacg aatgctttgg cttagccgcg cagacgctcc acatcacggg caatcattag
    10261 ttcctcatct gttgggatca ctaatactct tacttttgat tcttttgtag agatatctac
    10321 ttcttcacca cgcaattcat tcaacgctgg atcgatttca caaccaaacc aagacatacc
    10381 tttgatgatt tcactacgta catgtccgtc attctcgccg ataccagcag taaagacaat
    10441 tgcatcaaca ccattcatcg ttgtcacata accgccgatg tattttcgga tgcggtccac
    10501 gaaaatgtca taagctaaac gaatatgttc tttttcatag ttcttttcaa gatcacgcat
    10561 atcactagac aatcctgtta ggcctagcag accagatttc ttgttcaaaa cattgatcat
    10621 ttcatcaata tcaatctcta atttttccat taaatatggg agaacagctg gatcgatatc
    10681 acctgaacgt gtacccattg tcaccccagc taatggtgtg aatcccatag aagtatccat
    10741 ggatttacct ccatcgactg ctgtgatcga tacaccattt cctaagtggc aagtgatgat
    10801 ttttagttct tcgatcggtt tgttcaataa ttttgccgca cgttccgaca cataacgatg
    10861 acttgtgcca tggaagccat attttcttac tttgaaatct tcataataag acaccggtaa
    10921 gctatataaa taattttgct ttggcattgt tgagtggaaa gatgtatcga atacagccac
    10981 actcaaaatg tcaggtaaaa tctttttaaa cgctttgatc cccattaagt ttgcaggatt
    11041 atgcaaagga gcaaattctg ctaattcagc aatgcggtcc attactttct catcgatcac
    11101 tactgaatcc ccataagttt ctcctccgtg aacaacacgg tgccctactc cggtgatttc
    11161 atcataagag cttaaaattt gcagttcaat caactgatct aacagcattt ttactgctac
    11221 ttcgtggttt tcgatatcca tgatttgttc gaatttttga ttatcgccgt acttgatggt
    11281 aaaaatagaa tcttgtaaac cgatacgttc gacgatccct ttcgcaacta cggtttcttc
    11341 tggcatttga tataattgcc atttcaaact tgaactccca gcattgattg caattgtttt
    11401 agacatatga ctctccttct cgttgaatga ttacagagca gatgatttcc agtttttaaa
    11461 ctcgttgaaa aattccgtca ccttctctgg ttcttttaat gaagcaagtt tcacaagcaa
    11521 gacttctttt acttgttgtg cttgtggtcc ttttttctgt aagatcaaaa tacttttttg
    11581 cgattgttta ttacggaaca attcatctgg caattgaatc attccttgaa gataaccttc
    11641 ttctttaaac cattttttga tttcttcact ttgatccgtt tctaaaaatc cactcggcat
    11701 caaaaacaaa ccaaaaccat ccggcttcac atatttcatt gattgctcta gcaataaatg
    11761 atgtgcataa ctatggcctt cttcggtact tgttaaaaac tcttttgctt tttcgtcatt
    11821 cggataataa ccgattggca aatcgctgat tgcaaaatcg acaggatcta ttaataattc
    11881 ctgcaacccg tcttgatgaa agtattgaac atttgcttgt gtcaaatcac ttgtagaagc
    11941 tgcgacagcc aacaatgtat catcaatatc cacaccaatt ccttctgttt ggtaccccgc
    12001 attcgatagg tttaaaagaa ctgttaataa caagttgccc attcctgccg caatatccag
    12061 aatcttaacc ggtgcttttt tatttgtgta tagttgttca ataagaaaaa caaataaaaa
    12121 accgatacta tcaggagtga gttgatggtt cgcctgcaaa tgttctgtct ggctgccctt
    12181 caataaaagt aattgagaca gtctgcgcca ttcttctggc tcgaaagaaa gtttcttcaa
    12241 ctcttcataa agagctgtga tccgttgcgt tgtctctttt gtcggcactc catccaccac
    12301 acgtacttga taatcatcta ttaaattttc tgcattttcg acataggctt ctaaaaagga
    12361 agttcccaat gcattctgta aaagctgaat agcttccagg ttttgattaa acccttgttc
    12421 cattttttct ggtaacatgg tgcacctcat ttctagatat attataacaa ttttttcacc
    12481 tgtttaattc tatcgaacaa ttgacaaaat ttcaatcgtt ttcactgttt tttatttgtg
    12541 aaacggcaaa cattttttta ttaaaaacag tgttataaca gaaaagaggg cgttttcacg
    12601 aacattccat cattctgtac tagtatctgt atcatttttt acttcttctg tgaaccgaaa
    12661 gataaacggt gagacttcca cctctaagaa tagggactgt tgtacccact gatatgttac
    12721 agatccagtt gtgtagtaaa aaactccttc cgaactttcg gcaagcgtag actgttccga
    12781 taaagccatc tgcttcatta tttttgctat ataataattt ttgattcgta tattaagatc
    12841 cattgacaaa cggtagcttt ctaatgctgc catcaataaa aacgtacata aaagaaataa
    12901 aagcaagaca gaaaataaaa tagagccctt ataacatttt ctactgatcg ttttttgtcc
    12961 atatggcata tccatcttct ccattttcaa agtgtacata aaaagaaatt gtgtgttctt
    13021 tttccaataa ttgaagctca gtgatacttg ttaacatagg ttgatgtccg ccggaagttc
    13081 ttcttttacg tatcattgcc tgatatcttt cgatgatata ctgtttatcc tcgatcgtgt
    13141 agtgcagttg ctgatttttc acagagacat tgtgactgct cttgagttct tcttctaatt
    13201 gtgctaaaaa tattagccac tctttttgat ctttacgctg caaaaattca cttacttgga
    13261 tggtttgctg aatcaaaaga ttgaatgtga gcaatagact gctcaatatc atcagtccaa
    13321 ttagacattc aatcagcgta aatgctggaa ttacttgttt tttcaatact gatcgcttct
    13381 ttctccatat tgacaaacac tttttcgatt actccattat tatcgacaat ttggatatcg
    13441 tatgggatca tttcttccct tcgctctggt cttttgtgat acatccgctg cgtcttgata
    13501 tcttcataaa tcattcgaat aaatagcagt tcctccccac ttttcttttc tgcctgaagc
    13561 aaaaacagct ggccaaagct cactaagtag acacagacag aagcgatcat aagcgctgta
    13621 agggactcca acaaaacaaa tcctgtttta ttcaattctt tttgtatagc gaccactccc
    13681 tatctggaat tgatacgtaa tcgtccgctc tttttcttcc caataaaatt gataagcttt
    13741 tagtgaactt tcattcccgg ttctagcagc aaaagtaata gaagcatggc gtttcaaggt
    13801 aatctcatca ggaatatcta atacttccca atttatttta tctggattcg gaacatgaaa
    13861 aatgatttga ttctcttcac tattccaata aaagcctgtt tggatctgat tcacaatagc
    13921 gattttttgc gtagaataga ttcgtttttc aaattggccg aaaaattgat agactgccaa
    13981 ttcattcttc catgcagaaa atgcgagaac tggaaaagaa aggatcgttg taagtaataa
    14041 aatcagtaag agtgattcaa acaacgtata tcctgatgct aaaaccttac cttcaaggcc
    14101 gctgatagat ttctgcatgt tctcgtttga tatagccttc attcaacaat tcctccacag
    14161 taggaactct tccattattt tgaagcgcat agagctcttt ttgtgtgtct accaactgga
    14221 tgatcgcttc acgactttct tggtctacat gattcctata acgcgacaaa ttagggacga
    14281 acaacaacac taatacactg atgatcagta agacgattag catttcgatc aatgtaaaac
    14341 cgctataata agatattttt ctctttttca tggcaaaacc tcctctatgc ttccgtaaac
    14401 aggtaataaa acagcggcat aaactaataa aatcaaagcc gctacgccca aaaaaatgat
    14461 tggctgaata aaatgcatcc agtgatgaag acgtttgaaa agctgttttc ttgtccattc
    14521 gctgtaaaaa atcagttctt tacctaaact tccctttgcc tctccctgta aaacaatttt
    14581 actgaattct tctgatagaa aacgataatt ctggaactgc tcagccaatg acatacctgc
    14641 ctccaatcct tgaatcaact tagaggcaag atgctgaatc agactttctt gtttcgtttc
    14701 ctttaagcag actatgattt gccttaattc gaaaccttca tgaaaaagct ttccaatctc
    14761 tagtgacaaa taagccgatt gatacaagga atatatcggt ccaatcaata attgtctgct
    14821 tatccattca ctctgatcaa caggagacaa cttcatcaga cgatacctta ctagtaaagc
    14881 caatagagta accgctagga tgaatagtag aacataccaa tgaaagactt gtatcagctg
    14941 aataccccaa tgctcttgca tgaccattcc tgaaccaata agctgaggca aaacgaacaa
    15001 ccgcatacta aacaagatac ctagcaaaaa ggtgagcaat agcatcggat atgcaagcag
    15061 ctttttcaaa tcattgcgaa atccttcaac aagccgaaac tgtttggcaa tttccaacaa
    15121 agttgtcggc agattcccat gacgttcagc taactcgatc tgtaccagtt gctctgctcc
    15181 atatcctatt tgcgaaagta cctcactaaa cgcttgccct tcctttagct gatgttggat
    15241 atttgccaaa ataacaggtg gaaacgaacg gatcgtgagt aataaataaa gggattcttg
    15301 cagactaaaa ccagataaaa gcagttctcc catggtttgt gcaaactggt tttgctggct
    15361 tcttgttaat cctttagaat agttctgact gccaaatatt ttcctgagct tcttcataac
    15421 tttcctccca tgattttttt actgattctt cctccataaa ttgataactc cataaaacag
    15481 aaggatggtg attggcatct tccaaaagtt cttgataaat gacaccagct aaacattcag
    15541 atagctcttc ttttttcttt gtcagttcat ggattctgac caatgtacct tcaagattgc
    15601 gtgcatgtac ggttgcaaaa acccggtgcc ctgttagtgc tgctcttacc gcagcatgtg
    15661 cagttgattc atctcttatc tcgccaatga ttagcaagtc tggacgatgc cgtaatgcga
    15721 gtctcaaaag tgtatcataa gtctgtgcta ttttcggatt gatctgtagc tgcagaaaaa
    15781 acggttcttc tatctctaca ggatcttcga tagtaatcac ttgcaatttt tcttccaaag
    15841 ccaaccgata cattagagaa gtcttgcctg atccgactgg tccgctgaaa aggtataatc
    15901 cacgtctgac ggttttctgt aaaatagttg caaaatctcg agaactaaag caacgatggg
    15961 gtgtttcaaa agtaccgtat aatagccgaa tcactaaact ctctcgttga aggtaatctc
    16021 ctacagaaga caaccgcaga cgaacttcct gtttttctaa agaataagta aatgcaccta
    16081 actgagcttt tcttttttct ccgatatcca tctgtcccaa atacttaaat cttgagatca
    16141 gttgctgtcc atctgccgaa gagactttct tccagaatgt tcgttttttt ccatagcgaa
    16201 aatagatgta aaatacttct gaagctggca atatatatac gtcttgggca tgtttggcaa
    16261 aactttcact tatcatttga tccgataatc gttttatttc cattattctc ccctccacaa
    16321 aaaacatacg tctttttgat ctttttgttt caaataaaat gaatttacaa aaaaatattg
    16381 gcaaaaaccg aatttatgtt atacttataa aaatttatct tgggaggcac tccattggaa
    16441 acaacattaa ggaaatatat cgaagtagca agtaaaaaaa agcctgctga cctagtgatc
    16501 aagcatgcta agatcgtcaa cgtctttacg aaagaaatca tggaaaaaga tgtagcaatc
    16561 tgcgaaggaa tgatcgtagg aatcggtgaa tacgaaggaa aaagcgtaca taatgctaat
    16621 gggcaatatc tagtgccagg attcatcgat ggccatgtgc atatcgaaag ctcactttta
    16681 tcaccaagcg aattttcaaa agtatccttg cttcatggag ttactactgt agtgacagat
    16741 ccccatgaaa taggaaacat tgctgggagt acaggtcttg agtttatgat agaagatgca
    16801 agacaaacac tcatgaatat cttttttatg ctaccttctt gtgtacctgt aacccctttt
    16861 gagaccaatg gtgcaaattt aaatgcaaaa aaattagccc cttttttaca aaaacccgaa
    16921 gttttaggct tagctgaagt catgaactat caagcagtag caaataatga aacagacatc
    16981 ctagaaaaga tccaattgat gcaccagcat aagaaaaaaa ttgacgggca tgcagctggt
    17041 atcggcatgg aagagttaaa tgtttatcct gcagcaggta ttcggacgga tcatgaagct
    17101 acaactgcaa aagaagctaa agaacgtttg gatcttggta tgtatttaat ggttcgagaa
    17161 gggaccgttg caaaagatct ggcatcgcta ctaccggcga tcacacctga aaattcaaga
    17221 agatgcttct ttgtcaccga tgataaactg atcaatgatt tagtaaaaga aggatcaatc
    17281 gatcatatcg tccgtcaagc tattgcactt ggaatcgatc cattacaagc cattcagatg
    17341 gcaacattga atgcagcaga atgttttgat ttgaaaacac taggtgcgat tgcccctggg
    17401 tatcaagctg atttcttctt aacagacgat cttaccaagc tgccaattca tgaagttttc
    17461 tataagggaa cattagttgt tcaatctggg caactccaag accatttatt tgctgatcat
    17521 tcaaattcat atacgtgttg tctgccaaaa ctaaacgctc aaccattaaa tccacattcg
    17581 ttagagctgc ttctttcttc ttcacaagcc catgtgatcg aaattatccc gaatagtcta
    17641 gtgactaaag atttggtaga aaaagtggac cgacaaggcg aacgattcat tccttcaatc
    17701 gaaaaagacc aattaaagat tgcggtgatc gagcgtcacc atcaaacggg aaacattgga
    17761 ttaggcatag tgaaaggatt tcagctgaaa caaggagcga tagcgacaac gattgcccat
    17821 gactcgcata atctagttgt cgttgggaca aacgacgagg atatgctgta tgctgctaat
    17881 cttttgattc aaaaaggtgg cgggatgacc gttgtcaaca aacagcagga aatagcttgt
    17941 gtttctcttc ctataggcgg aatcatgaca caggaacctt tttcgatcgt ctatgaacag
    18001 ttgaatgaat taataactaa agcacatcaa ctaggagcag ctaaaacctt tgatcctttt
    18061 ttgacacttt ctttcttaac attatctgtc atacccgaat tgaagatcac cgacaaagga
    18121 ttattttcat tttctcgctt ccaattgatt ccaccttgtg gaaaaaccga tgaaataaat
    18181 acattttaaa tcaaataaat cccagaaaat acattacatc tgtaatgttc attttctggg
    18241 atttatttat ggatattaat gtttttatga aagcagaaag ttagtttcac agacttttct
    18301 ttttggtctt atcttgaaat tgagcttcta ctcgataaat acgttgacct tttgaagaaa
    18361 atttttcttc atactccgtc ataatattgc cttcaaattg actttgatgt aaatctaacc
    18421 atacctgttt gaggatcatg ccatattgag aaaaacttgc cagagaatac tcaaacaatc
    18481 cttgattatc tgttttgaaa tggatttctc catttggacg aagaatctgt tcatctacag
    18541 caagaaaagt cttatatgtc aatcgccgtt tttcatgacg tttttttggc catggatcgg
    18601 aaaaattcag atagatttga tctacttcgc tgtcagcgaa atactctgtc aacgctgatc
    18661 catccacatg tagaagtttc aggttaggaa gcggttgttc gatcaacttg tctagtgcaa
    18721 tcgaaacaac actgacctgc atttctactc caatataatt gatttcagga tgggcttttg
    18781 ccattccagt gatgaactgg cctttcccca taccaatctc aatgtggatt ggatgatcgt
    18841 tttcaaacaa ttcattccat tttcccttcc acaatgttgg atcactaatt acaaaattcg
    18901 ggtgagccgc taacatttcc gctgctcccg gacgatttcg gacacgcatt atttatctcc
    18961 attcctttcc tctaattaaa aaggtctggc caaatctccg cagagtttgg gccaaacctc
    19021 agccactgaa aattcgtttt aattgtaaaa tttcttcgtt catccgtcga tgatcttcac
    19081 gtttgcaata tctggtgatt tcttggagca aattcatggc cgcataccaa tgaaattttt
    19141 caagcatttc gtcagtcggg cgtatgccat aactgatcag ccactgattc cagctagaca
    19201 gtggcacata atttcctaaa atcgttgcaa tatccaccgc agggtctgca aacatgattg
    19261 aatcccaatc gactagatat aaataatttt tacatactaa ccaattacga tggtttacat
    19321 ctccgtgtac tactgataag ttttccttat ctattgacgg gatctgctca cgaagaaaac
    19381 gatggacaat ttgcagaaat tgatttttct ggagttcttc cggtaattct ttttcatatt
    19441 tttgcagcat cttctccggc gtcatgacac gaccaccgat acgggtcagc atggttttaa
    19501 gagaatttga atgatgtaaa tggtacaaaa catcaatcac gtcgtttctt tgaccaatct
    19561 cattaggagt cagcaagcga ccttccagcc actcttgtgc tgtcaaagta tcgccattcc
    19621 ccgttcgttt tgtccaaaca agttttggtg taattccttc ccttgaaaga gcagccaaca
    19681 tcggagtcgt gtttcgttta ataaagactt tgtcctcatc acgcatgcct atgtatgttt
    19741 ttcctgtgtc gcctttgatg ggacgtaacc gccactcttg gtctaactga aactccatca
    19801 tgcattctcc cgtcctctta ctctttgttt tgcagtactt attattctag tcgcctgaac
    19861 actacacgtc aagcacatat acgttctttt tcatttaatt gccattcaaa aaaccagctt
    19921 ttctcaaaaa aaatagaacg aacagcattc ttactgtcaa cgctctattt ttcattctca
    19981 tttttaccct acacagcttt gttttcagtc tgacatcttc ttcaaacgag ttggtacata
    20041 tagtttaaca aagagcatag taacaatcaa ataagccgct agcggaaccc atcgctgctg
    20101 cccatctaat acgactgcag caatcaaacc aaaaataacc gacgcgataa ttaataccca
    20161 tgtgagtaga tactgaatcg cctgcttctt ctgattctta tggattggat aaagctgaac
    20221 gagaaccata tagcggaact ggttcgctaa aggtaacagt tggaaaccga tcaaatataa
    20281 aaataagaaa ccgataatca gactaaaata aaagtcgact actccggcaa tcaataaccc
    20341 acctataatt actaatcgga aataaagacc actaaaatca gaacctcttg ccaatcttct
    20401 tgcaaacaag taaagataag tgttttttga ttctctttta atatgagtca atataggatc
    20461 caaatagcgt cttcttttga tctttgccgt aatttcagga acatctgtga ataaattgat
    20521 aaattgatag atctgatgca acctatgttc ttctacatgc accattttct cccaatccaa
    20581 tcctttttct tgttgcttcc acaaaaacca gtaatataga cttgcctgta ctaatgcacc
    20641 tgctattcct gcccatatta gccaataaaa tgatagaaac aaaaagatca gagaagaaaa
    20701 aaaccagcct aatttccaat aacgaataac ttgttgatct agtcgaaaca attctgctct
    20761 cttgatttgc agatgactga atttcaaact ccataatatg atcagataga cgaaaaacat
    20821 cgaaaaggat tgctccgtac taaccaccac taatggcatg gccaatccgc atgccaatat
    20881 caaaaagatc aacggaaaaa tgcaagaata gcgaaaagct cgttccaaat aggtttgcat
    20941 ttctttttct ttaggaagta aaaaaacaat atctgctgcc tctgcaaaag tagccatctt
    21001 acccacgaat aaacagagca tccaaaatac agagattatg agccctccaa ttgcaaacgg
    21061 tggctgcagg gttttcaacc aattagaata atataatccc acaccgccaa tcaagaaaag
    21121 acaaactaaa acaaaatgat cgttcaatac ataacgcata tatttcatca ttttcttttg
    21181 atgtcttgct aaacgttttt gaaaaaaact actcatgtgc ctgctccttt gttaaagcaa
    21241 tatagatttc gtccaaagaa gaatccggca gctgaaattc ttttctcaac atctcaagag
    21301 aaccgtctgc tcgtatctct ccgttatgta agaccacaaa acgatcacaa tatttttctg
    21361 cagtagctaa aatatgcgta gacatcaaaa taccggctcc ttcttttttc atttctacca
    21421 tcaattcaag caatgcattg atagctagtg gatccaagcc taaaaatggt tcgtcgatga
    21481 tatataaaga aggctcgatc aaaaaggcgc ataagatcat cactttctgt ttcattccct
    21541 ttgaaaaatt agctgggaac cagtcaagac gattagataa ccgaaaagtc tccaacaact
    21601 tttctgctct tttcattgct atatccatag gtatatcgta ggccatcgca gtgacttcga
    21661 tatgttctct caatgtcaat tcttcatata aagaaggagt ctctggaata tacccaattt
    21721 gtttgcgata ttcttccgta tttttcttta aggatagatg attgatttct atcgttcctt
    21781 ttgaagcttc cagcagaccg ataatgtgct tgatggtcgt acttttccct gctccgttca
    21841 gtccaatcaa ccctactatc tctccaggct gtacatcgaa actgatatct tttaacacag
    21901 ggagatgtcc gtacccccct gtgacatgat cgataattaa actcattctt tttcctccgc
    21961 catttatttt tatttattat accacgaaga aaatgacggt attttgtttt tttccttaca
    22021 atagctttaa cttatggtaa agttaagctg aagtatttat tctttataga aaggatgttt
    22081 tatatggaaa actgtatttt ttgcaaaatc atcaatcaag aaatcccaag ctataaaata
    22141 tatgaagacg acaaggtcta tgctttttta gacatctctc aagcaacgaa aggacacaca
    22201 ttagttgtgc ctaaacgtca tgtagcagat atttttgaat atgatcccga acttgcagga
    22261 gaggtttttt cgcgtgtgcc taagattgct caagcattgg aaaaagcttt tccagaaatg
    22321 aaaggtctta atatcttgaa taacaaccgt gaactagcct atcaatctgt ttttcattca
    22381 catatccatt tagtccctcg ctacagcaaa gaggatgatt tttctattca ctttgtcaat
    22441 catcaagata gttatggatc ggaagaacta aaagctattc aagaaacaat cgtaaagcag
    22501 gtgtcctgcg atgattaaaa actttttcaa aggactgtta tttggagctg ctgctggcac
    22561 tgtcggaggt ctgttattgg cgcccaggag cgggaatgaa acaagaaaga aactgatcga
    22621 agaattggaa gaagcaacaa ctctgacaaa tgatttaaat aacagcctga atcattttaa
    22681 aagcgcattg atcgaaacaa aagaaacagc tgaatctgtg attcctgctt tccaagaagc
    22741 aatcaaaaaa gatatcgaag attttaaatt ccaagccgaa ccacgtatca cacaaatcaa
    22801 tgaacaagta gaaaaactaa aagctgatct gccagagtat cctgaacaaa tataataatg
    22861 attattttct caacagattt aactatataa gtgagaaaag tttgcgtatt tcctaggaat
    22921 gtgatgaaat tttgaatttt gtttgaaact accacgacaa cgtttttaaa ggaaatggga
    22981 tatggtatag ttaatatata aaaataataa ctaattacag gagtgcaaat tttaatgaaa
    23041 aaaaaatcaa ttattttggc agctactagt gcgttggctg ttttaactct agcagcttgt
    23101 tcaggcgata ctaataaaga tatcgcaacg atgaaaggcg gaacgatcac tgtatctgat
    23161 ttctatgatg aagcaaaact tgaatcatcg aaccaatcct tagtacaacg gatgatcatt
    23221 tataaagtat tcaataacaa atatggcgat aaagtaaccg ataagcaagt ggatgcagaa
    23281 tacgacaaac aagcgaaatc attaggtgat acttttgaat cacaattaga agctgctgga
    23341 tatacgaaag atacttataa agaatatatt cgtaacaatt tagcttttga agctggcttg
    23401 aaagctcacg tagatattac agatgacgat ttgaaaactg cttggaaatc tttccatcca
    23461 gaagtagaag cacaaatcat caaattatca agtgaagatg aagcaaaaga tgtgaaaaaa
    23521 tcagcggatg atggagatga tttctctaaa ttagccaaag acaaatcaac tgatacagag
    23581 acaaaagaag atggcggtaa agttaaattc gactcaacga caacaactat tcctgcagaa
    23641 gtaaaagaag cagctttcaa attgaaagac ggtgagatct ccgatgttat tactacaaca
    23701 aatcctactt cttatgcgac agaatactac gttgtcaaaa tggtgaaaaa ccaaaataaa
    23761 ggcaatgaca tggataaata caaagatcaa ctaaaagata tcgctactga aactaaacta
    23821 agtgacaatg cattcacaac taaagtaatc ggtgaagaat taaaagatgc aaatgtaaaa
    23881 atcaaagatg atgcctttga aaatgtgttg agtgcattca caacaacttc atcaagtacg
    23941 aaagactcaa gtgaaactac agcttcaaca aagagttctg acacaaaatc aactgattca
    24001 acaaaagaat cttcaactga agaaacaact gattcatcaa aataaaagaa aacaaaaaaa
    24061 gcgaagactt atgtctcgct ttttttgttt tgaatgaaaa aaataaattt ttgctataaa
    24121 tcttttggta catagaaact tcggttgtcc ataccgaaaa tccgatcggt gtattttcct
    24181 ggttctgttt gacgaatcga agtcaacatc atctgcatcg aagcatcgat attgtcgatt
    24241 tgatgtaata tttctgcttc cattattctt ggacgtacag gtgagccata ttctaataag
    24301 ccatggtgtg ctaaaaccat atgacgtaat acgagaacat cttcgtcagc ttcgctgatt
    24361 ttcaacgcaa gacaggcttt agttatttct tcgtcaatca ataccagatg accgatcaaa
    24421 tttcctgcaa gcgtatattc tgtcgacata ggaccggaaa gttcaatcac ctttcctaaa
    24481 tcatgcaaaa tgataccagc atacaacaat gctcgattca gctcaggata ttcatcggcg
    24541 attgatttcc ctaagtgaag cattgtcaaa gtatgataag ctaatccact ggcaaatgca
    24601 tgatggtttc tttttgctgc cggaaactca aaaaattgtt tttgatattt attaagtaga
    24661 tagcgaacaa tacgattcca atgcgcattg gtgatctcaa acaacaattg attgatttct
    24721 tcttccattt cttctttctt caaaggggct ctttccatgt ataaactagg ctctcctggc
    24781 tcatctaccc ttgttaagcg catatgaaga atctttactt gagggtttcc ttgataaacc
    24841 tctcttttac cgtttagaaa aacgactgtg ccggctgtaa atttattgat ttcctcctcc
    24901 gaagcatccc agtatttacc atcgatagta cctgaagtat cttggaaagt aaaagcaata
    24961 aatttttttc cgtttttagc gattcttaca tctgcacttt tgatcaggac aaattgttca
    25021 aactgctcat cgattactaa atcacgaatt tttttcatta tttgctcctt ctaattgttt
    25081 tacgatttgg ttcatctcat ggtaatagct taccatttct ttatcagaag aaaaacaaat
    25141 tatttgatac ttttctccaa atttagctaa aagttcagct aattgatact ttcgctgatg
    25201 atcataatga agccagccat catcaatgat gatcgggcat atcgaacggt tagcttctaa
    25261 tgagaggtaa gcaaatctca tcgccataat cagctgatct ttcgtacctg tagataactc
    25321 atagatggga aattctgtat atccgtctgt taaagtcagt gtatcatttt tcagtaatat
    25381 cttctgataa tttccttttg ttaaaatacc taaataatca cctgctgctc ttaacaactg
    25441 gggtaattgt ctttcactca tctctgttga aagatcacct aagaatgtcc cggcgatttc
    25501 aagtgctccc cattcctcca gtaattcctg taattttgct ttttgccggc tagcttgttg
    25561 ataaagttcg tccaatgtgc cgtctctttg caactcttct acttgaagtt ggagtcgttg
    25621 ccgattttca cttgcttctt tttcttcttg ctggagttgg gattgctttt ctttcagttg
    25681 gcgcagtctt acagataatt tctcttttgt cagctcttca ggaaatagtg gagtaagcat
    25741 ttccgataat tcattgtatc ttttcatctg ctgttcggct tgtttggctt tttgtaaaaa
    25801 gacaggtact tccgaaggat aagataagcc tgcttgagtc aattgttcgc tgtattcttg
    25861 tatccatact ttttgtttct ttttcaattg attgatttct tgcttcaata aagtattttg
    25921 ctgatagctt ctggcaaatt tcatctgttc catctcttgt ataaaacggg aaagcaatac
    25981 cattttttct gaaattgtct tatcttgtat cggtaaccat tctttaacaa actcaaattt
    26041 ttggtcgatt tgtcgtagtt tttcttgtat ttcctgttct cttttatgtt tccgctcaaa
    26101 tacgccatat aatttctgat aatctatgac ttcctgtcca tattctttca tgagtttgag
    26161 cgtatccatc tttcctaagt gccgatgatc cgcaaaacga tatacttctt gctgaagaat
    26221 ctgtttttct tctccagttc tcttttgttt ttcttctgct tcagctaatt gagctaaggc
    26281 tagatcaagt tgtcctagtt tttcttgcca cattcctttt atctcagaga aatcattttt
    26341 agttttaatt tttggttgcc ttttgtagaa gaaatagcca atcatgcctg caccaattag
    26401 taaaaggatc actttcaagg gcattggtac aaatatacct gcaatgatta ttgctccgcc
    26461 agccatcagc atatacggtt tagatgaaga gactggtgct atctctgact gattttgtaa
    26521 taatgctgga taggtctctt ccaagtcatt cacttctgct tcaatcatct cttgctgttc
    26581 tttagctatt tggagacgca cagaaatctg gctttctaga tcagcaatcc ttctttcttc
    26641 agccagaaga tgcttccatt cttcttcata taaaaattct tccggtaacg gatttttctg
    26701 ccaatgccac ctttgttcca aaatcttcaa ttctgaattt gtttctctag tctcaatttg
    26761 taatctttgc cattcttcgt ttaattgttc cgctgtaaat tgaagttgct ggagatcttt
    26821 tagttcctgc tcctgctcta aataaaaata atatctggct gactgctgat ccatcccgct
    26881 atgcttttcc agtgttgctt ctaattcttc caaacgctca tttgtttgct gataccgttt
    26941 ggagaaagac tccagatcta aaagaagttg ctcatcagga agacctgtag tgctgttttt
    27001 tttgagagat tgccattctt catataaaga aaaattaagt tcttgctgtc tggctaatga
    27061 aaattgtttg ttcgcctctt ttaattgtgc caataactgc tgctgttgcg actcgtattc
    27121 ctgttcttgt tggatcaact tgccaaaata agcttcctgt tcttgttttt gatgaatttt
    27181 ttcttgcagc tctttccatt catttaattt ttgattgatt agtaattttt ggcctttttt
    27241 cttgaataat tgttgtgctt tctgataata ttcttgccgc ttttggaata cttgagaact
    27301 gcccgtgatg cctaaagaaa tcagcgcatc atgtagctct ttttctctca aatgatctag
    27361 ttccatcaac tgctcttgct ggaaagtaaa tacctgctgg aataactctt tcgtcaaagg
    27421 tgcgatcagt ttttctagta actcatcact tcccacttga tcccccagcc acaccttcga
    27481 ttttccgcga ttttgctgct tgtagcgctc gacagcaaat tcaccatata caggatgttt
    27541 aagccatatt ttgccgccat aggctgagcc gtcttttggt gtataatcct tttttttacg
    27601 gccttttgaa gggaaaccaa ataatattgc ttgaataaat tgataaatcg ttgattttcc
    27661 tgcttcgttt cccccataga tcagctgatt gcctgaataa aagtcaaaac ttttttgacg
    27721 ccattttcca aaagcagcaa tctcgatcct taaaatcttc attcctgatc ctcctcaaat
    27781 aaaaaatctt gattaagcag ctgcttggct ttttcaagtg tttcttcttg atattccggc
    27841 agttcgctca atattctagc cgcttcttga tgactatata cttcatttaa aatttgttgg
    27901 aaatcttgag gtgtctgata gtattgaaat aactgttcca tcaatttagc agaagcagca
    27961 agtggggtct tcgtataaaa agtattctct atgagagaaa tacgccaaat aaagaaatca
    28021 tttatcctat ctgagaattc ctctaacaaa tagtctaata gctctcctga ttgtatccgt
    28081 tccaaaacat ctgtattgag atggtcataa tcttttaatt ttatctcaat taaactaaat
    28141 ccatcctcta cttttgaaag ctgctgacga atgtgaacaa tgacctcttt tgtcgttctt
    28201 gctttcctca atgatatttc ctttgtcttc caatagacct ctgctacttt aatcggatgt
    28261 atttggcaac ttcctttact taattcaact agtagaatag atgtagaagc ttgttccttt
    28321 ttagtatgac cttgaggcgc acctggataa acaattgttt gcttttcatt taagaccgtt
    28381 ggcacatgga tatgccctaa agcccaataa tcgtatccct tttcttgcat ctttgagggt
    28441 tggaaaggag catagtttcc tttttgagag acgccaggtt ccccgtggta caacccaata
    28501 tggtaatctg tgagttctct agaaggaaat tccatcactt tgtctttttg aatccacggt
    28561 tgacgataac taaatccact gagagagaca gtttctccac tttttatcgt tatttttttt
    28621 gtctctaccg tttcggatgt aaataaatgg acatttttag gaaaggagaa ccaataccgc
    28681 tctttttgat agaaatcatg atttccgaat attataaata caggtatatg gttcttttct
    28741 aaccgtttca tctgttctga aaaatgtttc tgtattttta atgaaggacg attttgatga
    28801 aaattatctc ctgcaagtaa aacaaaatca acgttattta ttattgcctg ttcaatgata
    28861 tttgataaaa cggtcagatt cgtcttcaat agtttttcct gtacagtttt atcaagagtt
    28921 gtcagtcctt caaatgaccg atccatatgt aaatctgccg catggataaa tttcaccatt
    28981 aaaaatttcc tccttttttt cttttatcat accattttca atgttttttt tcatcaataa
    29041 ccgaaaattt gttcgtatta tgcaaagaaa ttacgataaa tggccgactt caaaaaataa
    29101 gtttattcga aaaaaagatt gtgacatgat ttttgtcaca atcctaaata tctatgttat
    29161 attcgcttcg tttgacactg aagaagtact atttgtactg gaatttatgc tccatttttc
    29221 cgtatttaga attctatgtt ctatactacc ttaattagtt tatatggtaa gaattctttc
    29281 aaatgcttga gatactccgt ctttatcatt tgaaacagtg acataatcag cttttttcat
    29341 gatttcttga tgactatttc ccattgcaaa actaatatct gcactttcaa acatagataa
    29401 gtcatttaaa ctatctccga tcgcgattct caaaggtttc tttaaattgt agattgattc
    29461 caaacgatca aacaccacac ctttagatac attttgaggt acgatttcaa tatttacagg
    29521 tgccgattca gtaacagaca catttagcat ctttttcaaa tggttaaata tattagcttt
    29581 caattgacga ttaggaataa aaataaaaaa tttgtaaatg ctgatagcat ccttttctat
    29641 aatttcctca atattattat aagggattga
//
LOCUS       DAHHPA010000025        29569 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.026, whole genome shotgun sequence.
ACCESSION   DAHHPA010000025 DAHHPA010000000
VERSION     DAHHPA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.026"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            116..985
                     /locus_tag="NBH55_001037"
     CDS             116..985
                     /locus_tag="NBH55_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCD1509035.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1150..2421
                     /locus_tag="NBH55_001038"
     CDS             1150..2421
                     /locus_tag="NBH55_001038"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD1509036.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2418..3713
                     /gene="rho"
                     /locus_tag="NBH55_001039"
     CDS             2418..3713
                     /gene="rho"
                     /locus_tag="NBH55_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCD1509037.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3812..4075
                     /locus_tag="NBH55_001040"
     CDS             3812..4075
                     /locus_tag="NBH55_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCD1509038.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4520..4969
                     /locus_tag="NBH55_001041"
     CDS             4520..4969
                     /locus_tag="NBH55_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1509039.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            5026..6444
                     /locus_tag="NBH55_001042"
     CDS             5026..6444
                     /locus_tag="NBH55_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1509040.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6444..7304
                     /locus_tag="NBH55_001043"
     CDS             6444..7304
                     /locus_tag="NBH55_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCD1509041.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7478..8251
                     /locus_tag="NBH55_001044"
     CDS             7478..8251
                     /locus_tag="NBH55_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1509042.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8398..8763
                     /locus_tag="NBH55_001045"
     CDS             8398..8763
                     /locus_tag="NBH55_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCD1509043.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8911..10119)
                     /locus_tag="NBH55_001046"
     CDS             complement(8911..10119)
                     /locus_tag="NBH55_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1509044.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10138..10416)
                     /locus_tag="NBH55_001047"
     CDS             complement(10138..10416)
                     /locus_tag="NBH55_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509045.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10570..11265
                     /locus_tag="NBH55_001048"
     CDS             10570..11265
                     /locus_tag="NBH55_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCD1509046.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(11707..12504)
                     /locus_tag="NBH55_001049"
     CDS             complement(11707..12504)
                     /locus_tag="NBH55_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCD1509047.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12501..13685)
                     /locus_tag="NBH55_001050"
     CDS             complement(12501..13685)
                     /locus_tag="NBH55_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCD1509048.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13710..14957)
                     /locus_tag="NBH55_001051"
     CDS             complement(13710..14957)
                     /locus_tag="NBH55_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCD1509049.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15172..16917
                     /gene="dnaX"
                     /locus_tag="NBH55_001052"
     CDS             15172..16917
                     /gene="dnaX"
                     /locus_tag="NBH55_001052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCD1509050.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16937..17251
                     /locus_tag="NBH55_001053"
     CDS             16937..17251
                     /locus_tag="NBH55_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCD1509051.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17276..17872
                     /gene="recR"
                     /locus_tag="NBH55_001054"
     CDS             17276..17872
                     /gene="recR"
                     /locus_tag="NBH55_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCD1509052.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17936..18580
                     /locus_tag="NBH55_001055"
     CDS             17936..18580
                     /locus_tag="NBH55_001055"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCD1509053.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18595..18924
                     /locus_tag="NBH55_001056"
     CDS             18595..18924
                     /locus_tag="NBH55_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCD1509054.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18938..19876
                     /gene="holB"
                     /locus_tag="NBH55_001057"
     CDS             18938..19876
                     /gene="holB"
                     /locus_tag="NBH55_001057"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCD1509055.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19912..20736
                     /locus_tag="NBH55_001058"
     CDS             19912..20736
                     /locus_tag="NBH55_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCD1509056.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20729..21076
                     /locus_tag="NBH55_001059"
     CDS             20729..21076
                     /locus_tag="NBH55_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCD1509057.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21145..22017
                     /gene="rsmI"
                     /locus_tag="NBH55_001060"
     CDS             21145..22017
                     /gene="rsmI"
                     /locus_tag="NBH55_001060"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCD1509058.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22126..23247
                     /gene="dinB"
                     /locus_tag="NBH55_001061"
     CDS             22126..23247
                     /gene="dinB"
                     /locus_tag="NBH55_001061"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCD1509059.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23301..23903)
                     /gene="nrdG"
                     /locus_tag="NBH55_001062"
     CDS             complement(23301..23903)
                     /gene="nrdG"
                     /locus_tag="NBH55_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCD1509060.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24218..26407)
                     /gene="nrdD"
                     /locus_tag="NBH55_001063"
     CDS             complement(24218..26407)
                     /gene="nrdD"
                     /locus_tag="NBH55_001063"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCD1509061.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26706..27500
                     /locus_tag="NBH55_001064"
     CDS             26706..27500
                     /locus_tag="NBH55_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509062.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27490..29532
                     /locus_tag="NBH55_001065"
     CDS             27490..29532
                     /locus_tag="NBH55_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509063.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         9479 a         5316 c         6189 g         8585 t
ORIGIN      
        1 ttttcatttt cattgatttt agtagaaaac taccgaaatg tttggtaaaa tagggtcgtt
       61 ggttaagaat tgtcttaaca aatttagttt tacaaaactt aggaggaatt ttattatgcc
      121 agtagtatca ggagctgaat ttttaaaagc tgctcgtaaa ggcggttacg ctgtcggcgg
      181 attcaacaca aacaacttgg aatggactca agcaatcctt gaagcggctg aagcaaaaca
      241 agcaccagta cttatccaaa catcaatggg tgcagctaaa tacatgggtg gttacaaaat
      301 ctgtaaagat ttaatcactg atttagttga agcaatgaac atcactgttc cagttgctat
      361 ccacttagac catggtgatt acgaagctgc tttggaatgt atcgaattag gctatacttc
      421 agtaatgttc gatggttcac atctaccatt tgaagaaaac ttgaaattag ctaaagacgt
      481 tgttgaaaaa gcacatgcaa aaggtatctc agttgaatgt gaagttggtt caatcggcgg
      541 agaagaagac ggaatcatcg gtaacggcga attagctgat attgaagaat gcaaacaaat
      601 ggttgctaca ggtatcgact acttagcttg cggtatcggt aacatccatg gtcaatatcc
      661 agaaaactgg aaaggattag cttttgacca cttacaagca attgctgatg ctgtaggtcc
      721 agacatgcct ttagtattac atggcggatc aggtattcct caagatcaaa tcgaaaaagc
      781 tatctcaatg ggtatctcaa aagtaaatgt taacactgaa ttccaattat catttgctgc
      841 agctactcgt aaatatatcg aagctggtaa agataaagaa ggtaaaggat tcgaccctcg
      901 taaattatta gctccaggta aagcagcaat cgttgaagat gctaaatctc atatcgactg
      961 gttcggttca gctaacaaag cgtaagcatc cacataacag atacgatata taggaatgga
     1021 gaacgacttt tgaagtcgct ctccattctt ttttacttaa aacggcagac catgctcgtt
     1081 tctttttttt atctatgata aaatacaagc atatccatga aaaaagatta ataaacaagg
     1141 tggaaacaga tgaaaaaaat cgtaatcaaa ggaaaccgac ctttagctgg tgaagtaacg
     1201 atcagcggtg caaaaaatag tgcagtcgca ttgattccag ctgcaatctt agctgattcc
     1261 ccagtgatct tggaaggtgt gccggacata caagatgttc attcattaat tgaaatttta
     1321 gagatcatgg gcgcaactgt ccatttttcc aacaacatac tagaaatcga tcctcgagaa
     1381 gtcgtatcga ttccgatgcc gcacggaaaa atcaatagtt tgcgtgcttc ttattatttt
     1441 atgggaacac tgcttggtaa atttggcgaa gcggtagtag gcctgccagg tggctgttat
     1501 ttaggcccta gaccgattga cctacacgtc aaaggatttg aagctctagg tgctgaagtg
     1561 acaaatgaac atggtgccat gtatttacgg acagaaaata aaggattgcg tggcaatcgg
     1621 attttcatgg acgtggtttc agtcggagca acgatcaatg tgatgttagc tgctgtaaaa
     1681 gcaaaaggac agacagtaat tgaaaacgct gcacgagaac cggaaatcat agatgtggca
     1741 actttactga ataatatggg ggcaaaagtt cgaggagcgg gtacagatgt gatccgtatc
     1801 gaaggagtcg aaacattaca tggctgtcgg catttcatga ttcctgaccg catcgaagca
     1861 ggaacctatc tagcattagc agctgcagta ggaaatggta tcaaagtgaa aaatgtgatc
     1921 tttgaacatt tggaaagctt tatagcaaaa ttgcaggaaa ttggtgtcca tatgaaaatc
     1981 agcgaagatg aaatcgaagt ttacccctcg aaagaattaa aacccgcaaa catcatgacc
     2041 tatccttatc caggatttgc cacagatctg cagcagccac ttactgcgtt actattgatg
     2101 actacaggca cttcagaaat catcgatacg atctatgcga aacgtgtgaa ccatgtgccg
     2161 gaactagcac gaatgggcgc tgatattacc gtggaaggaa atatgatcat cgtgaatggt
     2221 ccaaataaac ttcacggcac agaagtcgtg gcttctgatc ttcgggcagg tgcttgttta
     2281 gtgattgctg gactattggc agaagggaca acgacgattt acaatgtaga ttatatctta
     2341 cgcggttatg atcatattat cgagaaacta acagccttag gggcatcaat tgaaatgata
     2401 gaggaagtgg gagctgaatg agcgattatt tgacaatggc agaactagag aacaaaacgt
     2461 taaaagaagt ttatagttat gccaaagaat ttaaaattcc ttattacagt aagatgaata
     2521 aaaaggaatt atctctagct gtgattcgtg cacaagcaga gaaacaagga ttttattata
     2581 tggaaggaat attggatatc gttggtcaag atggttacgg ttttttacgg ccaatcaatt
     2641 atggaccaag tgcagaagat atctatattt ctgcctcaca aatcagacga ttcagtttga
     2701 gaaatgggga caaagtggca ggaaaagccc gtccacctaa agaatcggaa cgttattatg
     2761 gtttgatgca cgtggaaagc gttaatggaa aagatccgga agaagcaaaa gaacgtccgc
     2821 attttccagc cttaacacct ctttatccgc aaagacagct tcaactggaa acaacaccgc
     2881 aaagactatc tacccgaatg atcgatatct tttctccaat cggttttggt cagcgtggat
     2941 tgatcgtcgc accgccaaaa gcagggaaaa cttctgtatt aaaagaaatc gcaaatggta
     3001 tcaccgaaaa ctatccggaa gtagaattaa tcgttctact gattgatgaa cgaccggaag
     3061 aagtaacgga tctagaacga agcgtgaaag gcgacgtggt atcttctaca tttgatctgc
     3121 agccgcaaaa ccatactcga gtttctgaat tagtattgga acgcgcaatg cgtttagtcg
     3181 aagataaacg agatgtcgtt atcttgatgg atagtattac tagacttgct cgggcctata
     3241 acttagtcgt tccaccaagc ggacgtacat tgagtggcgg gattgaccca gccgcattgt
     3301 ataaacctaa gaaatttttc ggggcagcaa gaaacatcga agaaggtggc agtttgacga
     3361 ttttagcgac cgctttagtc gatacaggaa gccgtatgga tgacgtgatc tatgaagaat
     3421 tcaaaggaac tggaaatcta gaattacaac tttcacgtga attatctgaa cgtcggatct
     3481 tcccagccat cgatatcaaa aaatcaggta cccgaaaaga agaattattg atgccaagtg
     3541 aagaattaga agaaatttgg aaacttcgaa aaaatatgac tggagattca ttagaatata
     3601 cggaacaact gatccgcttt ttgcgtaaaa caaaaaacaa tcagcaattt ttcaaagaat
     3661 ttcaagatgt ctcctttggt aaaaaaaatc cgaccagaaa tctgaaaaga taattgcaac
     3721 cttgactgaa acatgttaag atgttaatgt tgtaaatcga caaatcaact ctggttcagc
     3781 aaatggagca gggcaaagga gcgaaaaaat catgaaagaa aacatccatc cagattacca
     3841 cccagtagta ttcatggatt caacaactgg tttcaaattc ttgtcaggtt caacaaaaac
     3901 ttcacaagaa acagttgaat gggaagacgg taatacatac ccagtcatcc gtgtcgaagt
     3961 aacttctgac tcacatccat tctacactgg acgtcaaaaa ttcacacaag cagacggacg
     4021 tgtggaccgt ttcaacaaaa aatacggtct caaagacgca aacgctgcgg aataataaaa
     4081 acgctgataa ctcaacgttt agcagactat ctgataggga tagtctgttt ttttatgttt
     4141 tttgccaatt taaaacttcg tttgatgctg aaaaagtcgt aggatcataa tgcacaaaac
     4201 aatgaaaaac tagtatatag ttttaattat ccacttgata aaaacacctt atattaacga
     4261 aatgaagagg ggttatttcg aagtaaaaaa ccatttacct aaaattagaa gctaagcttt
     4321 cctttcaagg aaggcttttt gttttgttat agacttgcga ttgtttgtga gctagatcgt
     4381 tcattttaat gacttataca cgcggatgat gggtcttttt cctctgttca ttcataatat
     4441 tgaacggtac agctagcgat gctatactga cctcagttcc aagaaaacgt taacattttt
     4501 tgtgggggag aggtttttta tgaggtttga tcaattgatc gcagaagaac tgattgtttt
     4561 cgatccatcg atagattcaa aaggatcatt atttgagcga ataggggatg tgttgaaaaa
     4621 aacaaataag gtaagaaatg ttcaaaaagt cgttaaggca ctattcaaac gagaggagga
     4681 gatctccaca ggaatagaag atggatttgg gattccccat gcaaaaagca aatatgtgac
     4741 aaaaccagca ctgtgttttt ttcatacagc caaattaacg gactatagcg ggatagatga
     4801 aatgccaatt gaatatattt ttgccatcgt agttcctgaa aaatcagcag atatgcatct
     4861 agagattctc agcacactct ccagacgatt gatgaatgca gattttagaa agggaataaa
     4921 agaagccact acgccagcag atatcatcag acagattagt caagaatgaa aatagtagag
     4981 tctttgtccg agtatcgata gtgaagaaaa aggaggaaaa taatcatgaa aatcgttggt
     5041 gtaactgctt gcccgactgg aattgctcat acttatatgt cggcagaaaa attagaagta
     5101 acagcaagaa atctaaacca tcaagcaaaa ttcgaaacac aaggtgtaaa aacagaaaat
     5161 cagttatctg aacaagaaat caaagaggca gatgcaatca ttcttgcagt agataaagaa
     5221 atcgaattgg accgatttgc cgggaaaaaa gtaaagcgtg tctctacaag ccgagcaatc
     5281 aaagaaccgc aagtagtaat agacgaagca ttgagagata ttggcgtatt cgttgtgtct
     5341 aatgaaaaag aaccagctgc aaatgagaaa aagccgacaa tctataatca tttcatgaat
     5401 ggtgtgaatt acatgcttcc atttgtgatc gcaggcggaa tcatcatcgc actgagtttt
     5461 gcctttggga ttacagcggc tgatcccaac tcagcagact ataatgtgtt agcagctgca
     5521 ttttcaagga tcggaggaga tacagcgttt gccatgatgg ttcctgcgtt aggcgcgggg
     5581 attgctacat cgattgctgg aaaagcaggt tttgccccag gaattgttgc cggattactg
     5641 gcctctacag gtggttcagg ttttcttggc ggaatgattg gtggcgtgct agcagggtat
     5701 atctgtgatt ttctggcaaa taaaattagc gtaaaaaaag aattttctgc tatttatcag
     5761 ctgattgtcg tccccttttt atcgatctta attattgggc ttttgatggt atttgtgatt
     5821 gaacaaccga tcacatgggt gttggatgct ttgacaaatt ggctgaatag cttgggaaat
     5881 acatccgggc tattgttcgg attacttatc gggatcatga tggcagctga tatgggtggg
     5941 ccgatcaata aatcgacttc gactttctcg atcggtttga tgtcagctgg agtaaatgca
     6001 ccgattgctg cttgtatggc tgcaggaatg acacctcccc taggtattgc tttagccact
     6061 ttattattca aacataaatt taccaaagaa gaacggactt caggtcaatc atgctggatt
     6121 ttaggcgctt cgtatattac agaaggtgct ataccgtttg cggtatctga cccgatccgc
     6181 gtgataccta gtttgatgac aggttcagca gtcgctgcag ggatttccat ggcagtagga
     6241 gttacttcaa tggcacctca tggagggatt tgggtgatgc taattccaaa tgttatgaac
     6301 aatttaccta tgtatgtctt ggcaattgca tcaggtacag tcactacagc ctttatgctt
     6361 ggactattga aaaaaacagt gaaagagaaa aaacaagaca agtggacgaa aaatgaaaat
     6421 ctaacaaatg aggtggaagc gtaatgttat atacaatgaa agagttattg aaagtggcta
     6481 aagagaatca atttgcagta ccagccttca atatttgcag ctatgatatg atgaaagcaa
     6541 tcatcgaaga agcagagaga ttagaagcac cagtaatctt agaaattcat cctgatgaaa
     6601 tcgcctattt aagtgatgag tttgttgcgg cagtaagagc ctatgcttat acaagtaaac
     6661 tgccgatcgt tatccacttg gatcatggcg gtacgataga agatgtctta cgggcgattc
     6721 gaaacggcta ttcttccgtg atgatcgatg cgtctttgaa atcattcgac gacaatgtac
     6781 agattacgaa aaaagtggtt gcgttagctc atgcggtaaa tgtctcagtg gaggctgagc
     6841 ttggaacgat tgggaataat ggttctgctg aaggaggtgc tgaaacaatc atttatacag
     6901 atcctgatca agcgaagatt tttgtgaaag aaacaggaat cgatacactt gctgtagcaa
     6961 ttggaacagc gcatggcttg tatccgaaaa acagaactcc aaaattaaat atcgacttac
     7021 tgaaagaatt gaaccaacaa atcgatcttc catttgtttt acatggcggt tcaggtaatc
     7081 cggacgcaga agtcagcgaa gcagtaaaat acggagttgc caaagtgaat ttgagctcag
     7141 atcttaaaag cgtctttttc aatgctttga gagaaatatt gagtgaaaat ccatcgatgt
     7201 acgagccgaa tgcaatttat ccagcagcta ataaaaaggt acaagaagtg gtcaaacata
     7261 aaatggaaat tttgaacaca gtaggcaagg ctagtttata ctaaaaataa aaatgtgtgg
     7321 tcaagagggt atgacaatag tcttgtcata ccctcttaac cacacacgat gttcagaagt
     7381 agttttttcg aaaaatttct tcttatttct cgaagccaat cacttctgtc acaacctctt
     7441 ttttcctgtt actatataaa gtaaatgggg tgattttatg ttgaatgaag tggagaatag
     7501 acagcaggag attgtgaatt atctaaaaaa caaccaattt gcaagagtca ccgatttgat
     7561 cgagttagta aattatagcg aagccacgat taaacgtgac cttgtttttc tggaaaaaag
     7621 tggattgatc cgtagagttc gcggtggcgc catgcttatt gacaatcgaa aaattgacgt
     7681 gccttacctg atgaagatca ccaaattaga agaagaaaca gaaaaacgta agattgcaga
     7741 tacagcttct tctttgattc gagatgatat ggtcttattc ttggattcca gtacaacctc
     7801 tttacactta gtcagaaacc tagcaagatt tgatggtttg cagatcatta cgaatggcat
     7861 cgtcacagct gctatgttga gtgaatatac aacggcaaaa gtatctgtat tgggaggggc
     7921 aattgttccg aaacgagcaa caatcaacgg ttcaaaagcc tataacgatg cattgtcata
     7981 ttatgcggat ttagcgataa tgagttgtcg cgggctagat ttcagccaag gagtaacaga
     8041 aactcatgaa ggagaggcaa tggtcaaaaa agcttttaga aaacaatcag accggcttgc
     8101 cgttttagtt acacagcaaa aaattgcaca taaattcatg catcaagcca ttgcttttca
     8161 tgaaatcgat tatctggtga caagtaaaga actgacaaaa gaacagtatg cgaaagtaaa
     8221 ggaacaccat atcgaatgtc tgtacggata aattattacg tgaatgttct aagtggcttc
     8281 ctcattttta gataggacct tatctttgct cgcttctctc agccgatggt ataataatga
     8341 aaaggttatc ttgttagata atcttacttt tttatccagg agaaaggagt gcagaaaatg
     8401 aaacatgtga aggcgttggt agtgaaggca atcatgatct gggcgattct gtgggtcgtg
     8461 ctgacaggtt tatacggtgt tagctttatg gattcaacga ttgttggggt aattattgta
     8521 gtcatgatct atgtgctagg ggatctgttg atcttacgta aagtagggaa catcgcagcg
     8581 acgatcgcag atgctggttc agctgcagtt atcttgtggc tctatctata ttttatgaat
     8641 gatacagtcg atatttggat gaaagtattg attcctgctc tattgatcgg tgtggcagaa
     8701 tggttcttcc acaagtggtt gctaagtcaa ggcatcgtac cagatgaacg gaaaatggaa
     8761 taaatcaaca gttaaactat tcttatcgat aaatgaaagc tttaaaaata aatagatgtt
     8821 taattaagaa taatcataaa ctatttttac tcaaaaaacc gctcagatta ttttcagatc
     8881 tgaacggttt tttttatcta ttcatcaaga tcaaatttca tttttcaatg attcaatcaa
     8941 ttcgccctct gcttcatgac tatgttttcg gccattaaca agatagagtg ctgtacatat
     9001 caatggaatg actccggcaa tcacatacaa tcctctaaat gaagtaagag attttagcat
     9061 ccccatcaaa taaggaccaa cgcctaagcc taggtctaaa ccaataaagt aggtagataa
     9121 agcaatactg attcgatgtt cgcggacgat ttttaaacaa acagcctgtc catttgacat
     9181 aaacgtacca taacctaggc cgactaatgc tccagagata agcaatgtca cactattcgt
     9241 cgttacactc agtaaaaata atcctgctgt taagaataaa tagctaggat acatcacgta
     9301 ttgttctcct ttgacatcga aaatccttcc agtcaatgga cgtgtaaatg tgatgaccaa
     9361 agcatatacg acgaagaaaa atgacccagc agcaactaag tggattactt ttgtataaga
     9421 agataaaaat gataaaacac ttgaataaga aagacccatc aaaaaggcaa ttcctgtaat
     9481 gaaaagtgct ttcttttcaa taaaactatt gatattccat gagcgaagag atattttttg
     9541 ttcttcagat aatggaatat tttttacagg aaagacaagg caggcaatcg taacaaagaa
     9601 gaccagtaca attgaaaacc agataatgaa acgaaaatca gtcatattca gcaaaatcat
     9661 tcctaaaaat ggcccaacag cggcagctaa actagtactt aatccataat aattgatgcc
     9721 ttcccctttt ttagatgcag ggatatatgc agtaacgata gcatttgtag ctgtagaaac
     9781 tgtaccgtag ccgaatccat tcaagaatcg aactaaatac aatgccccga tagttggcgt
     9841 ataaagatat gctaatgttg aaaggagata gaagatcgcg ccgcctctta aggtaaactt
     9901 ccgaccaaat agttctaact gtttccccat gaataagcgt gccagcaatg ttccgatgat
     9961 atagatccca gaagcaaaac ctgcttggct caagctggcg tgtaaggtgt cttgcgcaat
    10021 gactgccatg atgaccatca acagataata aatcaggtag acaataaaat tgatcaatgt
    10081 gattccaata aaatatttat taaataattt cttttccatg tatgttatac attcccctta
    10141 agctgaatat gcttttaatt tttgtatttg aacttctaca tcgtgataat tggatggata
    10201 gcgatcaaca agttggcgaa taaataaatc tcttgtagac ataaacagat ttaaaaattc
    10261 ttcgcattcg tttgttggat tctcatccac tgctttgatc aattcgatcg agttttgata
    10321 cgcatcattt aaaaagacta aagaagcttt catatcaaat cttgtttgat atgctgtttc
    10381 taatcgatta atgattgtgt tcatccagta attcatgttt tttccctcta ttcttaaaaa
    10441 attttacttc tgcagtataa cgaataaaaa aaaggaaaaa aatcgcataa gtgatggaaa
    10501 aaggtactta acaaatgtta atatcattag taaaatacct ttggacttta aaaagtttta
    10561 aagtactgta tgatcttttt agatgaaagt ttggatgggg gagcatcatt ggatagtaga
    10621 gtcttaaagg aatatttgga taaaaatgat tttccgatta tacgcaaagg atatcataaa
    10681 tatttgacat tcgaagggat ggaagattcc catacgtata tcttgaaaga gggaacggta
    10741 aaagcaagtg ttatttcaaa tgatgggaga gaatttaacc ttcgctatat caaagatctt
    10801 gaaatcgtct ctcttttaaa agacgaatat tcacaattca tcgattcgcc gtataatatt
    10861 cgagttgaat cagaatatgc agagttttat cgaatcaatc gtgtgcgctt ttggcaaaat
    10921 atcaaccaag acccaaagct acaacactat gtaaaagagt attaccgcta tcgtctgctt
    10981 tattcgatga aaaaaatgca gcaaatgcta ttaaacggaa agctgggagc tgtttgtacg
    11041 caactttacg aattatatga tttattcggt gtgcggacag aagaagggat gctgatcgat
    11101 ttttcgatca cgaatgaaga aatcgccaaa ttttgtggca tctcaactgc tagcagtgta
    11161 aacagaattt taaaacaact gaaaaaagat ggtattatca ccgttaaaga ccatcatatc
    11221 gttattttga atatggactt gttagaagac aatattatct tttgatgaac aaagaggttg
    11281 tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
    11341 ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta ccaagaggtt
    11401 gtgagcctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag cttttcatgt
    11461 ttgatgaata ttgtgacttg tgaccgagga gttggtagga gaacaccgtt taccaagagg
    11521 ttgtgaaaaa gctgttctgc atcaagtaat aagaagagcc aagtaaaaac agtctcttat
    11581 gtttttaact tggctggact tatcaccgta gatgtaagct tttgaacgcc gtttatttgc
    11641 aacaaaaggg gctgtgacac gatttttgtc acagcccttt ttttgcgaac attcatttag
    11701 caatttttag aaacttctgc cgccaccgcc aaaagtcccg ccaccgctag aatgggtagt
    11761 acttccgcca ccgccgcttc caggaggtgg gcttttgggg attcttcttg tcgtgacaaa
    11821 agaatttgtc agacgatcgg ttttttcagt taatttgata ctggattttt ctcggaatgg
    11881 atatttgtag atgcctgatt tcaattgata acgcgaaaca gtcacaaaat aaaagacaag
    11941 actaaggata agagccaaaa caacagcaat gatgatttct actgtcgtca aaaccttata
    12001 gcgtgtaatt ttgccagttt cttcgtctac acgataatgt cctcctggaa ctccgtcttc
    12061 cacatatgtt gctgatttag acaggtaagc ttttgcagca ttgtaatagg cagcatttgt
    12121 catctgatca taaacatcat ctaagatcga atcgattctg ctatcagtca aataatcgat
    12181 catattacca gaagtcgaaa tgtagatttc acgctgtccc atatctaata gcaatactga
    12241 gccattttga tcatttccga ctgcgtctct taagtaatta tcagcaaagt ctcttggttc
    12301 ttctgaatta gatgtagtcg ttacgatcaa gactcgtcct ttgatttttt cattcagctt
    12361 ttctgcctgt tgttccaaag attggatttg atctgttgtg aataacccag cctcatcatt
    12421 taccgtagct gtatctgcat gagtagccac cggccagcca acacttacta gcaaaagaaa
    12481 gaataaaaaa aattttttca tcatatcaag taacctccga tcagaaagag gatcgctagc
    12541 ccagcgaaaa tcccaagagc ggtcagtccc aattttttct ggctgatcgg caggacaccg
    12601 ctgacctttc ctgtttgtcc attcattgcg tagtagtaga cttttttact tgagtcattg
    12661 cttcgataag taacaagcca taccggcagt aatacgtaat tatttacttc gtttgtgatt
    12721 gaagcagaag ttcgcacacc ggtcaatgtt gtatatccat ttgcggtatc ttttaacagc
    12781 ttctctgaat agtcttttaa ttcagattga atctgtgatt tgattgcctc atattcgata
    12841 tctctttttt ctgcttggaa cccggctaaa tactggcttt taaaatcaat tgctttatct
    12901 aacgggaaag gttgcacgcc ttcgaccatc ttttgttgcg tattttttga taaggcattt
    12961 tttactagtt ctttaaaaga aagcttgcct gctcttgaaa cagcaaattg cttcgtttct
    13021 gtgtattcga tgtcacctat ccgccatatg cggataactg taccattcgc ctgcatggca
    13081 ccatctactt cagcatctgt agcccaataa gggaaataca cacctgtcaa cttatcgatt
    13141 tgatccttat tgaaaaaagc tttgggaaca aaccattttt tcttggtcca agcgagaaat
    13201 ctgttgattg cttcttcttt ctcgacagca aaaggaagta ctttatttgg caagaattct
    13261 ccgcttaaac gtcccgatag tacgacggga ttatggcagt agtaacaata agtcgcagca
    13321 gttgtcgcat cagtcacgat ctctgcacca cagttgggac aaagaaaaag atctatcgct
    13381 gcggtattat ctgcagattc gtccgctgta tctgaaaaac cgcctgcctc gtcaaccgct
    13441 ttgcgctcgg cttcattcat atcggcaagc tgttcttggg ctgtaaaagt aaaatcttcg
    13501 gtaggtgaag gttcttcttt gctttcttcg ccagccatac gcgcttcttt ttgcttttgc
    13561 tcatattggc tgacttcttc ttctgtatag atattcaggc agtattcgca atgaaatttt
    13621 tggtctttgg gatcaaaagt caacggaccg ccgcaattag gacatttatg tgtaagaaca
    13681 tccatcagct gcacaccttt ctaaataaat taattaacgg ggattccttt aaacggttta
    13741 ccacagtttg gacagaattt agggatgccg ttggaaagat ccacgatctc attgcattca
    13801 ctgcatcgca tttgtagctt aggctgaacg ttttctacag gtttggcagc tccgcagttg
    13861 gaacagaatt ttccggaatt ttctgtcccg caaactggac atgtccaagt atccgcattt
    13921 ccttgtgcag tttgttgatt ttgtttttct gcttgttgtt tgatttgttc ttggttattt
    13981 tgagctgctt gagaaagata agaaccattt gcattcattc ccatacctac acccataaag
    14041 ccggtcattg ctcctgcttc attttttccg gctgcttcca taccacgtgc aatggaacct
    14101 tgcacgtatc cttcacgtac gctaggatca cctagcatcg ccccttcgtt acgcatattg
    14161 atcagtttca cagaatcatc ggtgtaagaa atgctggcta cagccacaga aacaatctcc
    14221 atcccacgta attcacgcca agaatcatcc aatgcagtat ccatgtattt gcttaattcc
    14281 aaactcttag atggtacata agaaatccgt tgaccatctg cagacatctg attgattgct
    14341 gattgtagcg ctgttaggaa ttcagctaaa tactgctcat tgatatcatt gatttccact
    14401 tgtgttttgt ttttagggat tgcgtttgta taaaataaaa tcgggtcaac gacatgaata
    14461 gaataagtgc catgtgcccg taagaataat tctgcgttat agaaattgtc aaagtagttc
    14521 aacggcgcgc ttgtaccgaa tttgattccc tttatttctt ggagattgat ataaaaaact
    14581 tgttgtttct gcggtgtgac gccgccaaat ttgaatcggt caaaagtttc agcaattgct
    14641 tctttcagtg ttccattgaa catggaagga gctgcgtcat ttttaatggt gtaataacct
    14701 tcctcagcgg tataatcgat gatttttcca ccatcgacca aaagcatcat catgtttgga
    14761 tagacatgga cgactgttcc gtctgttaaa acatcttctg ttcctttacg attggagcca
    14821 cgtttgtcat ctttgcggac tttgacacct tttgtcatga ccgttgtatc actcatattg
    14881 tctggttcga tgacttctag ccattgatct gccagaccgc cgccaaccat acttgttgct
    14941 gctttgataa gacccataaa aaattcctcc tctgattcag ctagtgccaa acaattattg
    15001 tttatttatt ataccatagg aagatgtgaa acttctcgga ctagagacgg aattgaaatg
    15061 gaaaattgcg tattgtttaa atacctgaaa aagccgagaa ggatagtagt ttttttacct
    15121 aatatcacgt ataataagag atagtgaatt ttttaaaaaa gggggatcct tatggcatat
    15181 caagcacttt atcgagtctg gcgttcgcag cgtttcgaag acatcgttgg acaaaaagct
    15241 gtgacacaga cactaaaaaa tgctattgtt tcccacaaaa cctcacatgc ctacttgttt
    15301 acaggaccca gaggaaccgg aaagacgagt gcagcaaaga ttttcgcaaa agcaatcaac
    15361 tgtccgaaca gtaaagacgg tgagccatgc aacgaatgcg aaatgtgtcg atcaattact
    15421 gctggtacgc aagaagatgt gatcgaaatc gatgctgcca gcaataacgg agtcgaagaa
    15481 atccgattta tccgtgatcg ggcaaattat gcgcctacaa aggcagcgta caaagtgtac
    15541 attattgatg aggtacatat gctttcaaca ggagctttca atgccttgtt gaaaacattg
    15601 gaagagccaa aagaaagtgt catattcatc ttagcaacga ccgaacctca caaaatacca
    15661 gctacgatca tttcacggac tcagcgattt gattttaaac ggatcaatac ggcagatatt
    15721 gtcgaacatc ttgaatttat cttgaaagaa tcgactatca gctatgaacc agctgcattg
    15781 aatgtgattg cccgttctgc agaaggcggg atgcgggatg cattgagtat tcttgatcag
    15841 gcaatctcgt ttagtgaagg aacaattact ttagatgatg ccatgcaagt gacaggtagc
    15901 ttaactgacg aaatgatgga cagttacttg agtgcttgcg tgaaaaaaga agtaccagaa
    15961 gcattggaaa cattgaacca aattctcgca gccggcaaag aatcacgtcg ctttctagag
    16021 gatatgttac aatactgccg tgacttgctg atgtatcagc aagcacctaa cctagttgag
    16081 gaacaatcga gtacgatcag aagtacattt acagaattgg cacaaactgt ttcagcggat
    16141 cagctgtttt caatgataaa gattttgagc gatactcaaa atgaaattcg ttttaccaat
    16201 agtgccgcta tttatttgga agtcgctaca gtcaaacttg cacagctacc gtctgcttct
    16261 gttccttcag cagatgtagc atttgcagat gatcagatag taaaacagct acaacaagag
    16321 attaaggaac taagaaacga aatcaaggaa ctacgcagta atacaggaat tgagaataca
    16381 aaagtacagc cggtacagca aacaaatcat aaaaaacaga caagtaccta tcgtgtaccg
    16441 acggaacgag tatataaagt tctgcgggaa gcgacaagac agcatctgaa ccaagtaaaa
    16501 gaagtatggg aagacttgct gatgagtcta tctgtcactc agcgagcaat gcttaaagca
    16561 agcgaaccag ttgcagctgg acctacagga ctagtgattg cgtttgatta tgagattgtt
    16621 tgtcaaagag cagcaaatga cgaagaattg cagctttcgg tacacaacaa cttaagccga
    16681 atgatcaaag attatgcgcc tgatcctgtt tatattacaa gagaaagctg gccagaactt
    16741 cgaagaaatt atttgacgca ggcacaagaa gagggaacag aaaactcttt tgcttctgaa
    16801 gactcagaag gagaaatcga gttgcttcct caagaacaag aagtaccaga agaaacactt
    16861 gtagtaaaag cagaggaact gtttggctcc gaagctgtta cagtggtaga tgattaataa
    16921 aaaggagtag ataattatga tgcgtggaat gggcaatatg caaggcatga tgaaacaagt
    16981 acaaaaaatg caaaaagaaa tggcagaagc acaagagctg ctaaataaaa cagagtttac
    17041 aggtaaagcc accaatgaat tggtcaaagc ggtttttaca ggcgatcgtc gcatgaaaga
    17101 tatccaaatc aatgaagaag tcgttgatcc tgatgatatc gagatgcttc aggatcttgt
    17161 cgtaatggca gtcaatgatg cgatcgcaca aattgatgtg gaaactgaga aaaaattagg
    17221 gaaatacgca aaaggtttgc caggatttta gttttaaact gaaggagtaa tgaatatgca
    17281 atatccagaa ccaattgcga aactgatcga cagctacatg aaacttccag gaatcggtca
    17341 gaaaacagcg acaagactcg ccttttatac gatcgatatg aaagatgaag tagtaaatga
    17401 atttgctaaa tctttgttaa gtgtaaaacg cgatttgcat ttttgtagta tctgcggaaa
    17461 catcacagaa gaagatccct gcgaaatatg tagaaatcct tcacgtgatc gaagcgttat
    17521 cctagtggtc gaagagccga aagatgtaat ggcaatggaa aaaatgcgag aatatcacgg
    17581 actttatcat gttctccatg gtgtgttatc accgatggaa gggacaggtc cagaagacat
    17641 caatatttct tctctgttgc agcggctgca tgatgcagat gtaaaagaag tgattatcgc
    17701 aacaaatgca acaacagagg gagaagcaac agctatgtat ctctcacgtc tgatcaaacc
    17761 cgcaggtatc aaagtgactc gtctagcaca tggtctgtct gtaggctctg atatcgagta
    17821 tgcagatgaa gttactttat tgaaagctgt cgaaggaaga cgtgagttat aaatttctaa
    17881 ttaagtgttt tttcagatac aatataaaag cagcacaaga gatgggggca acaatgtgaa
    17941 cggactattt attacaatcg aggggcctga tggtgcagga aaaaccagca tcttgaatga
    18001 actttaccct cttttgaaag aagtagcaaa aacagaaatc attcaaacaa gagaaccagg
    18061 aggcattccg attgctgaag agatccgagc agtgatcctt gatccaaaaa atgatcggat
    18121 ggatgaacgt acggaagcac tgctttatgc agcagcaaga agacaacatc tagtcgaaaa
    18181 ggttctgcct gctctggcaa aagggaaaat cgttttatgc gatagatttg tcgacagttc
    18241 tcttgcttat caaggtgcag gccgacgcat cggcgttacc gagattgctc ggttgaatga
    18301 gtttgctaca gaaggtacta cgccggattt taccctttat cttgacgtag attcagacac
    18361 cggattaaga agaatcaaga aaaacagaca aaatcaaatc gaccgtttag attcggaagg
    18421 tttggaattc caccagcgtg tacgtcatgc ttacttaaag ctggcagaag agaatccgga
    18481 aagaatccac aaagttgatg caagaaaaag ctttgaagaa gtgctgcaaa caagttatca
    18541 tacaattatt gaacagtatc cgcaattttt tgaaaactag gaaggtgaac gtttatgaaa
    18601 attattttag cgattatcca agacaaagac agcaaccgtt tatcaaatga attgattgat
    18661 gcaaatatcc gtgccacaaa actatcatcg acaggcggct tccttaaggc tggaaacagt
    18721 acgtttatcg tgggtattga agatgaccgt gtagaagaag cattggaaat catcaaaaaa
    18781 acgtgtgaat caagaaagca atttgtatct acacctgtta cactggatat ttcgatggat
    18841 gggggcgtcc cttatccagt agaagtagaa gtaggcggtg ccacagtctt cgtcttacct
    18901 gttgaaggat tccatcaatt ttaggaggag ctgagctatg aatcaagaaa taaacttggc
    18961 tcaaatgcaa ccgatcgtgt atcagcagct tcaacggagt tttgagcatg ggcgtatcgc
    19021 tcatgcttat ctttttgaag gggagaaagg aacaggaaaa catgaagtag gtatatggct
    19081 tgcacagcac ctgttctgta cccaaatgaa agacaatctg ccttgtggaa aatgcaacaa
    19141 ttgtcagcgg atcgaaaaga aagaacatcc agatgttttg gttattgaac cggaaggaca
    19201 aaccatcaaa gtcgatcaga tcagacgttt gcagacggaa ttcagtcgca gcggttacga
    19261 atcaagaaaa aaagtttttt tgatcaaaga agctgaaaaa atgaatagca gtgcggcaaa
    19321 cagtttgttg aaattcttgg aagagccccc tggagatttt ttagcggtgt tagaaacaga
    19381 tgcagtagga agaattctcc ctactattca atctcgttgt caagttctgc attttcaaga
    19441 actttcgaaa gaagcactga tccatcgatt gcaagaagag cagattccat tggaaaaagc
    19501 aaaacttttg gctttcttaa caaacagttt aggaaaagca gttgaaatct ctcaagatga
    19561 atggtttaat gatgctaagg acttgattta tcaatggttc gtttacttgc aaaagcaaga
    19621 tactcaagcc tttatctatg tccaaaaaaa attggtaaaa tcatttaaag aaaaaacaca
    19681 acagtttaca ggattatctg tcttgatgtt ttattatcag gaagcacagc gtcaagcagt
    19741 agcaaacggc gaatatggca aagtgaaaca gataaaccag ataattgaac ggatattgat
    19801 cgcagagcag aaactacgca gcaatgtcag ttttcaggct gttgcggaac agttcgtttt
    19861 gcatacgatt ttttagcttg ccgcaaagga tgatgatcaa ggaggatgga aatggtagaa
    19921 gtagtaggtg tccgatatag agatgcaggt catatctatt attttgcacc aggagactct
    19981 gaatatgttt atagtgacaa ggtattagtc gaatctcagc aatccttaca tattgctact
    20041 gttgtccttc ctaaaaaaga gattgatccc aatgaccttc ctgaagctat ccgtccgatc
    20101 ttgcataaag cgtcacaagc tgaattagaa aaagacaaaa aaaacaaaaa agatgcttat
    20161 gatgcattga aaatcgcaaa agaaaaagtg cagttgcgtg agttggaaat gaaaatggtc
    20221 aaagccgaat acacatttga tcgaaataaa ttgatttttt ctttcacagc ggacgggcga
    20281 attgacttta gagaattggt aaaagatctg gccgctgtat ttcgtactcg tattgagctt
    20341 cgacaaattg gtgtccgcga tgaagcaaag ataataggcg gaattggtcc atgtgggcgt
    20401 ccattatgct gttcttcttt tttaggcgat ttcgtgcctg tatcaatcaa aatggcaaaa
    20461 gatcaaagtt tatctttgaa tccaacaaaa atttccggtt tatgcggacg cttgatgtgc
    20521 tgtctgcaat atgagagcga agaatacgag acagcgaaaa aagaactccc tgactttgga
    20581 aaagtaattg ctacacctga tggtaaaggg aaagtcgtag ggttgaatct gcttagccgt
    20641 atcgttagta ttcggctgac tggacgggaa aatcctgtcg aatatgaatg ggatgaattg
    20701 aaagaattta tccaagtagg tgagatgaat ggataagaga tcattatacg acggattgaa
    20761 tcaattagaa tcagagttga gaaatacatt aacagaactt gttgaaatga agcatgcttt
    20821 gcatgagatc gtagaaaaaa atacaacttt ggagatggaa aatcaacgtc tgcgggaaca
    20881 cctgcgggaa ttgaaccaag ttgcacaagc tccagctgaa aatgtgaagc aggaactttc
    20941 taagtctcgt atgaatcttg aaaaaattta tgaagaagga ttccatgtct gctacgattt
    21001 atatggatca agaagggaca atgacgagcc ttgtgcgttt tgtctagaag tgatctatcg
    21061 cgaaagcggt cgatgaccaa taacaagagg agactggagc ggagaaatac tgctccagtc
    21121 tttttattaa ggagattgtg acagatgtat aatcaaaaaa gtttcaaggg aaatccggta
    21181 tatggggatt tatatcttgt accgacgcca attggaaatt tagaagatat gagctacaga
    21241 agtgttcgta tgctgcaaga atccgatctt atcgcaagtg aggatacgcg taatacacaa
    21301 aagttattga atcattttga aatccataca ccccaaaaaa gtttacatga acataattat
    21361 aaagaacgtg tccctcaatt gattgctttg ttaaaagaag gaaaaacgat tgctcaagtc
    21421 agtgacgcag gaatgccttc catcagcgat cctggacatg agctagtttt agcctgcata
    21481 aaagaaggga tttctgtgat ctctatccca ggacctacag ctgggatgac ggctttgatt
    21541 gcttcagggc tagtgcctca accattttta ttttatggct tccttccacg taagaaaaaa
    21601 gaacagaaag aaacattgaa aaagctaaaa gaacagacag ttacgctgat tttttatgag
    21661 tcaccttatc ggatcactgc tacattgacg aatttcttag aaatttttgg aaatcgccaa
    21721 gtcgttttgt gtcgggaatt gacaaaggtc catgaagaat accttagagg aagtacggaa
    21781 gagctactcg cttatatcga agaacatcca gtaaagggtg aatgttgtct gcttgtagaa
    21841 gggaatttgt tcgaaatgcc agtgacggaa gttgatgaag agttgggaac gttaaaggaa
    21901 caagtagaag caaagatcgc ttcaggcgaa aaaccaaatg ctgctatcaa agcagtagcg
    21961 ctgaaaaatg ggctgaaaaa acaagaagtc taccgtcagt accatgaatt agactgagat
    22021 agtataagtt gtctataatg aaagactaat gaggaaatca gctactaact tttcaaatct
    22081 cttgaacgcg aacgaatgtt cttgtataat gaataagagg tgaggatgat ggagctgcaa
    22141 tttcctttac ataatgatac atcaagaaaa atcattcata ttgatatgga tgcttttttc
    22201 gcttcggttg aagaacgaga tcatcctgag tttcgcggac atccattagt aattgccaga
    22261 caccccagtg acactggtgg gcgtggagta gtgactacag caaattatga agcaagaaaa
    22321 ttcggaattc attcagcaat gagtgcacaa aaagcgtatg agctttgtcc gcaagccatc
    22381 tttaagcctg gcgaccatca taaatacagc gatatttcca aacaagtacg ggaagtattc
    22441 tatcgttata cagatattgt ggaaccagtt agtatcgatg aagcgtatct tgatgtgacc
    22501 gaaaataaaa tcaattgcaa gtcagccatc aaaatcgccc gaatgatcca agcagatatt
    22561 tgggagagtg tccagctaac atgttctgcg ggagtaagtt acaataagtt tttggccaaa
    22621 ttagcatcag actatcaaaa acctagagga atcacggtag ttgcccctca agacgctgtg
    22681 ccatttttaa aagctttgcc aattgaaaaa tttcatgggg tagggaagaa aactgtacct
    22741 agaatgcatg agctagggat ttatacagga aaagacctat atgaatgcac agaaatgatg
    22801 ttgattcgaa attttggaaa aatgggctat tctctttatc gtaaagtcag aggaatccat
    22861 gattctccag taaacgttac aagagagcga aaatctgtgg gaaaagagca tacttatggg
    22921 cagccgttgc aaacggaaga agcagtgctg actcagttgc gccaactagc tgaaaaagta
    22981 gaagaagcac tgagaagagt ccaaaaacat gggaaaacag tagttttgaa ggtcaggtac
    23041 acggactatt ctactgtgac caaaagagtg acattgccag aatatatcta taaaaaagaa
    23101 gcactctttt atcaagcaag ccttatatgg gaggaaatct tgggagtaga aaaaggaatc
    23161 cgattgttag ggattacttt gacgaatctg gacccgatga cttatgaaaa catcgtcctt
    23221 ccattatggg aaaatcaaga aatataaaaa cagctttgag agttgaaacc ggaaatccgt
    23281 tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc ataggtttct
    23341 gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga tttttggacg
    23401 tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg tttgcttaat
    23461 tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt atcttctgaa
    23521 tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt tgtcgtaccg
    23581 aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa aaaaggttcg
    23641 ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat gatttgttct
    23701 tctaattcag gtgtgaatgg tttgccatat cggaaatttt gtgcagcttt gttgaagcag
    23761 ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc ttcaccatcg
    23821 acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt tgctaaccat
    23881 tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca aagatatagc
    23941 tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta aatccattgt
    24001 tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc catcgaaaga
    24061 gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt tcaaaagctt
    24121 gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt tgtttagcta
    24181 ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt ttcacacgtg
    24241 aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt cctaaatagc
    24301 cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat tctggacatt
    24361 cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag caatgatcga
    24421 ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat acagcttcta
    24481 aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat ccacctgaac
    24541 aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg tttttacgca
    24601 catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg atgatgccga
    24661 atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga gttgaataga
    24721 cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt aattctttga
    24781 cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca ccgaagaaag
    24841 ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga cggtttttga
    24901 ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca tacatatata
    24961 aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag agaagcgcat
    25021 ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga tctccttttg
    25081 attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt cgtccgacat
    25141 ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag cccataggta
    25201 ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca ggatacattc
    25261 ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct tcagggttta
    25321 agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc cgcttttcac
    25381 tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt tcaaaccagt
    25441 tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa gtaaataagg
    25501 tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg gtttttgctt
    25561 ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct tttagatgtt
    25621 ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg gcagaacagc
    25681 caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct gtggcggtct
    25741 ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg ttcagcatgc
    25801 ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa tccaaatcat
    25861 ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac attttcagtc
    25921 cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa acattactgt
    25981 ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt tcaatcgaaa
    26041 agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa ttgatgtact
    26101 cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg ttttgaatct
    26161 catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc aagtctgcaa
    26221 tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc ttttctgctt
    26281 tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca tcgcgtttga
    26341 tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct tcataattta
    26401 ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca aaatcgggga
    26461 aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt ggatttctat
    26521 actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct ggtggatttg
    26581 ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt attcagtcca
    26641 agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta tgcaaataaa
    26701 tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa gtgaaacatc
    26761 taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat atctcattca
    26821 cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga aagacaacgc
    26881 tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg atccaaggaa
    26941 aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa aaactaggat
    27001 tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat atcctattgc
    27061 ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga catgtagctc
    27121 aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct gtcggacaga
    27181 gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt tttgcagatg
    27241 aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac ttgaagacca
    27301 tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat acagcaagct
    27361 actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt gtcagaagag
    27421 ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt ggcggaggag
    27481 gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc acgaggctat
    27541 cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt cagcgcaatg
    27601 acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga tggtggattg
    27661 agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag tgccaaccga
    27721 tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg tatgaataaa
    27781 ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt cgcctgtatt
    27841 tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt ggttcgaatg
    27901 atggatatgg atcttactag tccatttttt atctccattc catcagtagt agatacttta
    27961 tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa aatatggcgc
    28021 tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac aatgcgcttc
    28081 aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag cggatatttt
    28141 ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta tcttgataca
    28201 ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac ttatttgttt
    28261 ttctgttata catttcggtt atttttgatc atattgagta aatggaagat ttattactat
    28321 ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag ttggcgtaca
    28381 aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg cacggctagt
    28441 atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta tcagtttgat
    28501 gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa gataactaaa
    28561 gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct cgaagaacct
    28621 atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag aaaagttaat
    28681 ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct tgatcaaatg
    28741 caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc agataatcaa
    28801 gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat ggtatatcta
    28861 agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt ggattatcaa
    28921 gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg actgagcaat
    28981 gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt aaatggaagt
    29041 ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga agcacaattt
    29101 agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg tgcagttaat
    29161 cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt gattattgct
    29221 actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa agaaaattat
    29281 cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat cttcgagcaa
    29341 aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt atttgcaatc
    29401 aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct tgtgagtggc
    29461 ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata ttgtcggatt
    29521 atcgaggaat gatatacgga ataagacaag ataataaata gaactaaat
//
LOCUS       DAHHPA010000026        28843 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.027, whole genome shotgun sequence.
ACCESSION   DAHHPA010000026 DAHHPA010000000
VERSION     DAHHPA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.027"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..1030)
                     /locus_tag="NBH55_001066"
     CDS             complement(<1..1030)
                     /locus_tag="NBH55_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1509064.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="NBH55_001067"
     CDS             complement(1112..1801)
                     /locus_tag="NBH55_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD1509065.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="NBH55_001068"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="NBH55_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCD1509066.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="NBH55_001069"
     CDS             complement(2328..2831)
                     /locus_tag="NBH55_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCD1509067.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="NBH55_001070"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="NBH55_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCD1509068.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="NBH55_001071"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="NBH55_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCD1509069.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="NBH55_001072"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="NBH55_001072"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCD1509070.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="NBH55_001073"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="NBH55_001073"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCD1509071.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="NBH55_001074"
     CDS             complement(6443..7462)
                     /locus_tag="NBH55_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCD1509072.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="NBH55_001075"
     CDS             complement(7635..8540)
                     /locus_tag="NBH55_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCD1509073.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="NBH55_001076"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="NBH55_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCD1509074.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="NBH55_001077"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="NBH55_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCD1509075.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="NBH55_001078"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="NBH55_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1509076.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="NBH55_001079"
     CDS             9753..10397
                     /locus_tag="NBH55_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD1509077.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="NBH55_001080"
     CDS             complement(10453..10956)
                     /locus_tag="NBH55_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCD1509078.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="NBH55_001081"
     CDS             11553..12518
                     /locus_tag="NBH55_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCD1509079.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="NBH55_001082"
     CDS             complement(12617..13789)
                     /locus_tag="NBH55_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509080.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="NBH55_001083"
     CDS             complement(13776..14921)
                     /locus_tag="NBH55_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509081.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="NBH55_001084"
     CDS             complement(14935..15867)
                     /locus_tag="NBH55_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509082.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="NBH55_001085"
     CDS             15984..17063
                     /locus_tag="NBH55_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCD1509083.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="NBH55_001086"
     CDS             17060..17689
                     /locus_tag="NBH55_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1509084.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="NBH55_001087"
     CDS             complement(17799..18026)
                     /locus_tag="NBH55_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1509085.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="NBH55_001088"
     CDS             complement(18129..20717)
                     /locus_tag="NBH55_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCD1509086.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="NBH55_001089"
     CDS             complement(20737..21396)
                     /locus_tag="NBH55_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD1509087.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="NBH55_001090"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="NBH55_001090"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCD1509088.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="NBH55_001091"
     CDS             complement(22169..23035)
                     /locus_tag="NBH55_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCD1509089.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="NBH55_001092"
     CDS             23144..23785
                     /locus_tag="NBH55_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCD1509090.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="NBH55_001093"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="NBH55_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCD1509091.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="NBH55_001094"
     CDS             complement(25226..26125)
                     /locus_tag="NBH55_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1509092.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="NBH55_001095"
     CDS             complement(26127..26924)
                     /locus_tag="NBH55_001095"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCD1509093.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="NBH55_001096"
     CDS             complement(26902..27606)
                     /locus_tag="NBH55_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCD1509094.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="NBH55_001097"
     CDS             27737..28312
                     /locus_tag="NBH55_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCD1509095.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..>28843
                     /locus_tag="NBH55_001098"
     CDS             28430..>28843
                     /locus_tag="NBH55_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCD1509096.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDF"
BASE COUNT         8475 a         6122 c         5186 g         9060 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttc
//
LOCUS       DAHHPA010000027        27725 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.028, whole genome shotgun sequence.
ACCESSION   DAHHPA010000027 DAHHPA010000000
VERSION     DAHHPA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.028"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            160..1419
                     /gene="lysA"
                     /locus_tag="NBH55_001099"
     CDS             160..1419
                     /gene="lysA"
                     /locus_tag="NBH55_001099"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCD1509097.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1406..2206
                     /gene="dapF"
                     /locus_tag="NBH55_001100"
     CDS             1406..2206
                     /gene="dapF"
                     /locus_tag="NBH55_001100"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCD1509098.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2209..3090
                     /locus_tag="NBH55_001101"
     CDS             2209..3090
                     /locus_tag="NBH55_001101"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCD1509099.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3087..3803
                     /locus_tag="NBH55_001102"
     CDS             3087..3803
                     /locus_tag="NBH55_001102"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCD1509100.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3809..4504
                     /gene="dapD"
                     /locus_tag="NBH55_001103"
     CDS             3809..4504
                     /gene="dapD"
                     /locus_tag="NBH55_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCD1509101.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4520..5521
                     /locus_tag="NBH55_001104"
     CDS             4520..5521
                     /locus_tag="NBH55_001104"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCD1509102.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5518..6879
                     /locus_tag="NBH55_001105"
     CDS             5518..6879
                     /locus_tag="NBH55_001105"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCD1509103.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6839..7990
                     /locus_tag="NBH55_001106"
     CDS             6839..7990
                     /locus_tag="NBH55_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1509104.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7965..9134
                     /locus_tag="NBH55_001107"
     CDS             7965..9134
                     /locus_tag="NBH55_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCD1509105.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9309..10688
                     /locus_tag="NBH55_001108"
     CDS             9309..10688
                     /locus_tag="NBH55_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD1509106.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10969..11766
                     /locus_tag="NBH55_001109"
                     /pseudo
     CDS             10969..11766
                     /locus_tag="NBH55_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11955..12623
                     /locus_tag="NBH55_001110"
     CDS             11955..12623
                     /locus_tag="NBH55_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCD1509107.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12823..13209)
                     /locus_tag="NBH55_001111"
     CDS             complement(12823..13209)
                     /locus_tag="NBH55_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509108.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13181..14563)
                     /locus_tag="NBH55_001112"
     CDS             complement(13181..14563)
                     /locus_tag="NBH55_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCD1509109.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14719..15906)
                     /locus_tag="NBH55_001113"
     CDS             complement(14719..15906)
                     /locus_tag="NBH55_001113"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCD1509110.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15913..16944)
                     /locus_tag="NBH55_001114"
     CDS             complement(15913..16944)
                     /locus_tag="NBH55_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCD1509111.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(16960..18414)
                     /locus_tag="NBH55_001115"
     CDS             complement(16960..18414)
                     /locus_tag="NBH55_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1509112.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            18589..19494
                     /locus_tag="NBH55_001116"
     CDS             18589..19494
                     /locus_tag="NBH55_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1509113.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19585..20262
                     /locus_tag="NBH55_001117"
     CDS             19585..20262
                     /locus_tag="NBH55_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD1509114.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20259..20429
                     /locus_tag="NBH55_001118"
     CDS             20259..20429
                     /locus_tag="NBH55_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509115.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            20543..21439
                     /locus_tag="NBH55_001119"
     CDS             20543..21439
                     /locus_tag="NBH55_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1509116.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21494..22864)
                     /locus_tag="NBH55_001120"
     CDS             complement(21494..22864)
                     /locus_tag="NBH55_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1509117.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVIRAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23102..23848
                     /locus_tag="NBH55_001121"
     CDS             23102..23848
                     /locus_tag="NBH55_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD1509118.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23845..24684
                     /locus_tag="NBH55_001122"
     CDS             23845..24684
                     /locus_tag="NBH55_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD1509119.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24685..25635
                     /locus_tag="NBH55_001123"
     CDS             24685..25635
                     /locus_tag="NBH55_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCD1509120.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25776..26528
                     /locus_tag="NBH55_001124"
     CDS             25776..26528
                     /locus_tag="NBH55_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509121.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26547..27698
                     /gene="queG"
                     /locus_tag="NBH55_001125"
     CDS             26547..27698
                     /gene="queG"
                     /locus_tag="NBH55_001125"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCD1509122.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9105 a         5048 c         5823 g         7749 t
ORIGIN      
        1 tgtttttata accattttta ttcttatttt taaaaaaata tttcgaaata tactagacaa
       61 ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc
      121 caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc
      181 ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa
      241 gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat
      301 acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt
      361 gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct
      421 gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc
      481 tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta
      541 ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc
      601 ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc
      661 atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat
      721 ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg
      781 ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg
      841 aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa
      901 gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc
      961 attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta
     1021 tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg
     1081 atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca
     1141 aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc
     1201 gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt
     1261 tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca
     1321 gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct
     1381 gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga
     1441 ttttatctta ttagattacg cagaagacac agattatagt caattagccg cttctttatg
     1501 cacaaaagaa aaatatgata cagacggatt gatcgccgta aaaatcgatc cgttggaaat
     1561 gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt
     1621 tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt
     1681 agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat
     1741 gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa
     1801 ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat
     1861 tcataccgtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact
     1921 gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa
     1981 tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg
     2041 tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca
     2101 ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat
     2161 ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt
     2221 tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg
     2281 gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca
     2341 acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca actgacagta
     2401 aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa
     2461 acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc
     2521 ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa
     2581 gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca
     2641 atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca
     2701 ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac
     2761 agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt
     2821 gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga
     2881 agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc
     2941 agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga
     3001 cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt
     3061 ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg
     3121 gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg
     3181 cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt
     3241 ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag
     3301 tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac
     3361 atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa
     3421 tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata
     3481 gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag
     3541 tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg
     3601 gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta
     3661 ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg
     3721 cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt
     3781 tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca
     3841 aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt
     3901 taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac
     3961 gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc
     4021 tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc
     4081 aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc
     4141 gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt
     4201 aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg
     4261 tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc
     4321 gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc
     4381 agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt
     4441 aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt
     4501 atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt
     4561 agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa
     4621 attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac
     4681 tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg
     4741 tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga
     4801 taattcctca gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct
     4861 ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt
     4921 acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta
     4981 tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga
     5041 cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca
     5101 gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat
     5161 tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg
     5221 acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga
     5281 gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc
     5341 gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac
     5401 ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc
     5461 tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg
     5521 agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc
     5581 aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct
     5641 ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct
     5701 taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt
     5761 caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag
     5821 aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg
     5881 actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt
     5941 ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca
     6001 tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa
     6061 gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg
     6121 caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga
     6181 atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct
     6241 tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc
     6301 cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac
     6361 gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc
     6421 actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt
     6481 tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg
     6541 atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa
     6601 acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg
     6661 ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta
     6721 gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta
     6781 ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat
     6841 gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa
     6901 gacaaaaaaa tatttatatg aacaagtaaa aaacagcggg gccgttatcc atgaaatcgg
     6961 cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac
     7021 agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga
     7081 aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga
     7141 ctttataact aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc
     7201 agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga
     7261 agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc
     7321 tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc
     7381 tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt
     7441 gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt
     7501 tggccaaatc caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg
     7561 aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt
     7621 agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc
     7681 tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt
     7741 agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt
     7801 atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga
     7861 tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct
     7921 tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca
     7981 atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc
     8041 aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc
     8101 ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg
     8161 gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg
     8221 gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg
     8281 cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt
     8341 attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt
     8401 ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat
     8461 cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg
     8521 caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg
     8581 atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc
     8641 cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag
     8701 tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc
     8761 tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg
     8821 tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc
     8881 aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca
     8941 aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg
     9001 caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga
     9061 tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt
     9121 taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt
     9181 ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat
     9241 aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg
     9301 tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat
     9361 cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag
     9421 gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag
     9481 atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt
     9541 taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg
     9601 catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca
     9661 aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat
     9721 acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg
     9781 tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag
     9841 aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga
     9901 cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa
     9961 tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc
    10021 cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga
    10081 tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc
    10141 cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa
    10201 tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt
    10261 ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct
    10321 ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag
    10381 atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag
    10441 gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag
    10501 gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc
    10561 aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg
    10621 accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg
    10681 tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta
    10741 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg
    10801 cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt
    10861 accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt
    10921 taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga
    10981 ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag
    11041 cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc
    11101 aataaaaaga aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg
    11161 cgtaaatcac gtcgctacaa ttcttatcga ggaaaagttg gagcagtagc caaaaatcgg
    11221 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
    11281 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
    11341 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
    11401 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
    11461 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
    11521 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
    11581 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
    11641 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
    11701 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
    11761 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
    11821 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
    11881 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
    11941 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
    12001 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
    12061 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
    12121 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
    12181 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
    12241 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
    12301 tgcgagcaat cttaagacag caagaatgct tttctcttga aaatgccaaa catgttatcc
    12361 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
    12421 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
    12481 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
    12541 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
    12601 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
    12661 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
    12721 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
    12781 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
    12841 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
    12901 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
    12961 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
    13021 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
    13081 tgcttcttct tcattttttt tgataccgtt gactagttct tccatcgcaa aagcagtccc
    13141 ccattttaat ccaaatggcc gatctcgata ctcgttatag tcaatgaatt tttttgctgt
    13201 ccgtctcatc ttttccacct tctaatccac tcataccacc tgcatgcccg ccaacgagag
    13261 aactacgtgc gatggcgcgt cctccttgca agagactatg cgcatggatg attgattgaa
    13321 agccgtattt ttttctaatc gtatcgacaa caaaatcaac tttcgcttca tttatctgct
    13381 tttcaggatc ttcaaaaagg ttaagctgta aacctgtcgc gtatacgagg tcggaacagt
    13441 tcacgcctac acgccgaata tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag
    13501 catctgctag atctctgcta gcatttgtcg cttcgatttt catttgctgg tgaaacccgg
    13561 tcttgccatt tggatcaaga tagcctaaag agtatcctac ccataagctg actacttgtg
    13621 tttttgctgc agatcgtctc aagcgagtgc cgacttggtc ggccatttct ttgataacga
    13681 tctcgatttc ttctcgtctt gtataatctc tatctaggat ttgactattt ccgatacttt
    13741 ttgattttgg tgtatattct tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat
    13801 aaagctgagt accgatgacc cctagcttgc ttttcaagtg ataataatcc gcgtgagcta
    13861 aatcataaac agaatgtata cccatttgtt tcagacgttt tgctgttcgt ttgccgattc
    13921 cccaaaagtc agtgatatct gagattttcc ataacttttc tggtacatct tcataacgcc
    13981 attcagcaaa catttgcttt ccgtcttttg agccattgtc taaggctagt ttagcaagta
    14041 gcgggttatc gccaattcct atagtcgtgt agattccggt ctgctcaaac acatctttat
    14101 ggatcagttt agccatgtcg tacgcatttg ttttaccaaa gagtgccatg ctatttgtta
    14161 catcaaggaa gctttcatcg acggaataga catgatggtt cgcttcgtct gcatattttt
    14221 tatagataga attgatttct ctgttctttt ccatataata ggccatcctt ggggcaacaa
    14281 tatataaatc acttggatag gggaagggaa gatctctggc acggctgata ttggtgatgc
    14341 cgtaagcttt ttttgctgca ggactggaag ccaagattaa tccgctaccc cgtgtttctg
    14401 tcgaatcgga cggataggac atgactacta atttagttgt aaggggatca agatcctttt
    14461 caacgcactc tacggaggcg taaaaagatt tgcagtcaat acataaaata tctcttgaag
    14521 gttctttttc ataaacaaat accggatttt gattcgccca cataacaatc acctcttcaa
    14581 cacaattaga acacacgttc tctttccgtg tcaataaaaa acagcaattt tcaaatacga
    14641 gcaaaaggct cacatttgga aattgctgag aggttatttt tatctatttg tttttagctg
    14701 agatcgttga tttttttatc aaacatgaac agcttctgga tcgatctttg ttgtcccatt
    14761 tccaatgtgt gcaacataaa attccggagc atagccgaca actttcatgt attcatcata
    14821 gacattgttt ttgaaggcag gtatattttc ttctttgaca agagcaatcg cacagccacc
    14881 aaatccagct cctgtcattc gtgcacctaa gacaccttct tgtttttgag cagcatctac
    14941 tagtgtatct aattcgatcc cagttacttc ataatcatag cgaagagagt ggtgagaatc
    15001 atttaacagt tgaccaaatg cttctaagtt cccagcttct aattcagctt ttgccttcaa
    15061 agtccgctga ttttcagaaa cggcatggcg agctctgcgt atcaatgtat catcgccgat
    15121 ttggtcaata gttgattcaa attcttcttc atctaagtct cctaaagaag agataggcaa
    15181 agttttttgc aaacgtgcca aggcttcttc gcattcgcta cggcgttcat tgtattttga
    15241 atctgccaat tcccgccgct tattcgtatt catgatgacg attgcataac catctagttt
    15301 cactggaacc atttcgtatt tcagcgtatt tgtatctaat aagattgcat gatctagctc
    15361 accgaagccg atggcaaact gatccatgat acctgaattg acaccgatga attcattttc
    15421 tactttttta cctgtttgga ctagttccaa gcgatctgtt tttaaatcaa acagatcttc
    15481 taatacaaca cctactaata attctaatga agcgctactg gaaagtcctg caccattagg
    15541 gattgttcct tccactaaca actcaaaccc atgatcgatt gagtgtccag cttcttttaa
    15601 tttaaggacc attcctttta cgtaatttgc ccagtttgct tttttatcat attctagctc
    15661 atctaaagta aaactaatga caccgatatc gtcaaagttt gtggaataaa ctaatatttt
    15721 attatcgtct cgaggtgctg ctacaccttg cgttccataa gtgatggaag cggggaagac
    15781 atggcctcca ttgtaatctg tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta
    15841 ttgtgtagtt tttttaggtc cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat
    15901 ttccatgatg agttattcct ttcttgtttt gattttgaat tctgtcactg tttcaaatac
    15961 tgatccggct tttaaatgaa tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt
    16021 ttgagtctca aatgtgatgc caccgtgatg tgccagtttt cggttgcgca tttctggtgt
    16081 ttctgtgccg aaatttgcag tgaaaataac cacactagaa gcatctgtag caatacctac
    16141 ttggattctt ttatcggggc tagtcaagcg agcggcttct ttacccaagc cggtttcttt
    16201 taagaaaaat ggatgatcga ttccgtcaac aagatttttt tgatccaaat cactagcaaa
    16261 aacggacgct aattttttag gtatttgaaa atcgaaagca cttcctgcag cattttcttt
    16321 tacccctata ggaatactat ctgttcgcaa aggagcataa gcttcgctat tgagccaaag
    16381 ctcatgttga tcgattgact gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt
    16441 cggattgaat aaagtatctt ggtcactggt gccgcgagag gtcactcgcc agatattatc
    16501 ttttgtcaaa gtatagcgga tttccacatc cattgtacct ggaaaaccat gttctccgtc
    16561 gggacttgta gtagtaaaaa tgacagaagc ttcattttcg ccattcaaaa tgacataaga
    16621 ccattttttt aattcaaatc caggagcacc accatgcaga ttatgtccag tttttggatc
    16681 tgtggctaat tggtaagttt ttccattgag agaaaagcgg ccattttcga ttctccctgc
    16741 ggtgcgaccg atagaagcac cgatatacgg atctttttcg atatattctt cagcggtgtc
    16801 aaatcccaaa actaattctc gatcatttga ttttaaactg acgattctag cacctaaatc
    16861 agagatagaa agtgctagct tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc
    16921 aaaatctttt tctgtaatct tcattttcaa gatcctttct taatgggaag gaactactgc
    16981 atcgtgaagt tcatcttcca ttgtatcagc tggagccaat ttgttttcca attcctcaac
    17041 gatttgtgca tgttttttct ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa
    17101 aatcatcaaa gcagcaggag catagaatgc ataagctttg aaagtcgtta cgccatgagc
    17161 agtgatatca gcagcagtag cattaccagt catcccagaa gcgatagcga caaaccctac
    17221 gatcccgtta gacaaagcac cagcgatttt atctagtaaa gggcgaattg atagagtaac
    17281 agcttcgtta cggataccat ttttccattg accatattca acagagtctg tgatcgtcat
    17341 caaagcggat aagaaaatca attgttgagg gaagtagaac aatactagcc cgatgataac
    17401 gattggcaaa cttgaacctg cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat
    17461 ccctccaagg taaacgtagc gacgagagat gacgcgtgta agcattggga atagtggaac
    17521 agctactaaa ccaagtaagg cagcaactag accaacgatc caaaattcag ttggttggcc
    17581 aagtacatat ttgaagaagt agaataatac ggcagttgtt gctacattgg caatcgcata
    17641 agcgatataa gcaagggcaa gccacatcaa ctgatcattt tgagcaactg ctttgaacac
    17701 atctttgatc ccagcttttt cattttgctg acggatcgca gtatcgactt cttttgtgcc
    17761 caaggcagtg ataatcgcag taccgcctga aataacagca gcaatcacac cgaaccagaa
    17821 ccagcctgaa gcgccttcaa tatgtttatg agtaacaaga taggtaaaga aagtaacgat
    17881 agggacaacg actagagtag taccatttgc acctagagaa gagccgaaac gagcaaacgt
    17941 agcaaatttt tcacgttcct ttgtttccgt actcaaagct ggaatcattg accaaaaagc
    18001 gatatcctta aatgaataaa aactatccaa taagatgaaa acgattgcaa ataatatgat
    18061 aaataaagtc gtattatgag tagctaagcc aaagaaattg gtaaagattg ctaccaagag
    18121 tacggaacta acaacgccgc cgataactaa ccatggtttg aatttaccgt attttgtacg
    18181 agtattatcg atgatgccac caatcaaagg atcaaaaata atttcaacta agcggatacc
    18241 tacaactaag cttgtaactg tagcaatcat ttttgcattc gttgccttat ctgttcctgc
    18301 gaacatctgg cttgtcacga ataccataaa ataagtgctt aatgtaacat agaatgtgtc
    18361 gtgtccaaaa gcgcctaaag cgtaggacac acgttggatc attttgcttt tcataatctg
    18421 tattcctctc ctgttcaata tataatacta agtaaaccgg ttacaacgaa taacaaagat
    18481 cttttaatga cttaaactta acaagaaatc gcttcctttt taatatattt ctgtgacttt
    18541 tatttaactt tttgttgcat tatactaaag gagtaaggag ggatagacgt ggagcaatcg
    18601 atttttttaa aaaaggaaca tcaacaagta gaaaaagaat tgtattttga tttttgtggt
    18661 ttctctaaaa cgatgccgtt tcattctttt ggtcctgcta ttcgcaatga ttacatcctg
    18721 catgtcgtga tggaaggcaa aggatcttat catgtgaaaa atcaacaatt ccaattgaaa
    18781 aaaggtgatc tgttcttgat tcgtccagga gactcgacgt tttacttatc agatggagaa
    18841 gatccgtgga tgtattgctg gctttcattt ggtgggagtg ctgcagaaga aatcattcgg
    18901 cagtcgcttt tcagagaaga ccaatacacg atggtctctt ctgacataca aaaatatatc
    18961 gacattattt tagaatgcat gaaagatacg gaagaaacct tggaaaatga attacagtta
    19021 acagcattaa cctaccgttt tttatcagtc cttttaaaag atggcggaaa ggtcagcggt
    19081 ggtgtacaaa aggttcattc ctctttagct gttgaaacag tcaaatacat cgaggcacat
    19141 tatgctgaaa aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa tcggagccat
    19201 ttgtcgcgag tattcaaaaa tcatatgggt accagtatca aggaatattt gatcggggta
    19261 cggatcaatc gaggggcgtt tcttctttct ctgacggatg aatccgtgga aagtattgcc
    19321 tatcaagtag ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga
    19381 gaaacggcta cgaactatcg taaacgtatg aaaaacgaaa attttaagaa tattgctttg
    19441 gataccttga aaaaacagtt ggaaaaacaa accgcagttt cttggacaac ctgattagtc
    19501 tggttaaaat gacaagcatc ggcaaaagaa acttgtggcg gttatgctac actgtaaagc
    19561 gaagactaaa aaggaggaaa agggatgcgt acacttatca tcattagcca tccaacgatc
    19621 gaagaatctt taaatcagca atttttcaaa gaagcttctg ctggtttacc agctacttgg
    19681 catcatttgg aatcaagtta tccagacgga aaaatagata tagagactga actacagctc
    19741 ttggcccagc atgatcggat cattttccag tttccgtttt actggtatag ttctcctgct
    19801 catttaaaaa tttggcaaga taatgtcttg gaacgagcag cacatgtatt aaaagggaaa
    19861 gaactaggaa tcgtattgac aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa
    19921 gaagaatata cgatttccga atttttgagg ccgtatcagc gaatcgcaaa taaattccat
    19981 atgacctatc ttccgccatt cgttttagca caatttatgt atttatccca ggaaaaacgt
    20041 tgggagaagc taattgctta tcagcagtat ctctcattgg aaggcaaacc atcgctgact
    20101 cagcggattg actggtttat tcaaagagta caggaaaatc agaaaatgca ggaagaagaa
    20161 tctgaaaaac aagcatatat cgtagaagca ctgactgagg caaaagagca gatcgaagac
    20221 ttgaccttta cactgcaaga aatgaaagga acttcattat gacaaatgat tggctgatcc
    20281 aacaattaga tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa
    20341 taaagaaact gatggctgag atagacgaac gaacaatgca gctgcaagga gaaatcgacg
    20401 gacaatcttg gaatcatctc aactggtgaa attttcttat aaataacaaa gataaaaatg
    20461 tgcacagcga caattatttg ctacaataag aatatgaagc gtttgataac gctattatac
    20521 ataaaaattg gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa atgttcggat
    20581 gacccgaaga agacagcaaa aacaggcagc tcgtaagcgg atgatcatga ttggggcagc
    20641 agttgccgtg tttttgatca tagcaggagg agttgcgggt ctatattttc atggaaaaaa
    20701 ttcgtcagaa gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa cagagacgaa
    20761 acaaacaagt gagtcatcaa ctaaaacaaa agagacacaa acttctgaag aaaaaacagc
    20821 aaatggtttg ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga
    20881 aaagacgcct gcattgattg aagaatacca gacagaaatt cagggaaatc aagagggaat
    20941 agctggttta tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga
    21001 agggatcaga aaattacgtg cagcgtatca ggcagcagaa aataaagacg gtgtagactt
    21061 agatactttg atcaatcaat tatcggctaa ttatacgaat catgtcgcta agatttccga
    21121 tatttatttg cagactagcg cctctcttca agcagagtca acaagcacac aagacacaac
    21181 atcttctgat tctgagacag cagagtcggt agaagcttct gaaatgcaag ctcgaacaac
    21241 acaagacaca acagattcct ctgaaactaa taccaacagc gcgacttcac aggctgcgac
    21301 aaccgtagtt ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt
    21361 agaaacgatc ttgtcgctga atgggatgag catggagaat tatttcctga ctcccggtga
    21421 gacgctaaaa ttgaattgat aaaagcaata aaaaagaacg aagcttccac taaggtgaag
    21481 cttcgttctt tttttatttt ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt
    21541 aaagcaagaa aacgagtcca ataatcaatg agattctcgt ttcaggattg ataaacatga
    21601 aaatcaatac gatagctaaa aaagcaagtg tcacataatt cgtataagga gatagtggca
    21661 ttttaaaagg atgatcatgc attttatctg cgtgcttctt tttgaagcga atctcgctga
    21721 tcaaaatcac tacccaagga accataccag gcaaaatact tgagctgtat acgaaaacga
    21781 aaatattttc tgactggttg atgaacattg gtagaagata atttaagatc agaccaatta
    21841 agattccacc tgagattgct acgacaggat aaaaaggcgc accgtgacga gatagtttca
    21901 gaaatttttt agacatcttt ccatttatgg caagtgtata gaccatacga cttgcgctga
    21961 aaatccctga attgcaacct gataatgccg ctctaatcac gacaaaatta atcagtcctg
    22021 cagcaaatgt aatgccgact ttagaaaaag tttgtacaaa aggtgaacca atctcactca
    22081 attgattcca cggatagata ctcacaatga taaaaatagc accaatataa aaaatcaaga
    22141 ttcgaccgat agtggactgg acagcttcaa tgatcgtctt tttaggattc tctgcttcac
    22201 cagcagtcat tccgatcagt tcgattcctt gataggaagc aacaacgatc gataatgcaa
    22261 agaaaaatcc tttgacgcct ccagtgaaga atccaccatt tttccaaaga tttgaaatcc
    22321 ctactgcctg accatgatta ccaaatccaa ataagattac cccaagacct gcaatgatca
    22381 tcaaaatgat cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct
    22441 tgacagaaat aagatttgcc agcataagaa acaagacgac aatgattccg ggaataattg
    22501 ccggcaaatc aggccaccaa taattcatat aagatcctac agcaatcact tcacttattc
    22561 ctacgacaat atattgaaaa atattactcc aagcggtcaa gtagccagcc acaggatgga
    22621 tgtactcgct cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc
    22681 ctaaagcacg cataatcaaa tacaaaaaga atcctgcaat cgcataggct cccatcactg
    22741 agggccctgt ccaacgtatg gttgaggagg cacccataaa cagaccgaca ccgatcgttc
    22801 cacctagtgc gatcatttgg atatgacggg cactaagact tcgagttaac tttttttctt
    22861 ccatacaaaa actcctttcg ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca
    22921 catgtttttc attattaggt tagccttatt gaactcttga tgtgattcta cagaaaattt
    22981 tcataaaatg cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt
    23041 ccattttagt taaactatat ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga
    23101 tttggaacgt acggccattg taattcaaga cctttcagcc gcttatcaag ggaaaactgt
    23161 tttgaaaact atcaacttga cgataaatcc acaaaaaatt accggtatta tcggaccaaa
    23221 cggtgcagga aaatcaacat ttatgaaatc tttgctagag ttgataccag ctactactgg
    23281 taaagtcacc ttttctggac agccggtaaa ctcagtccgt aaaaaaatag cctacgtgga
    23341 acagcgaagt gaattggatc tttcctttcc aattgatgta ttaggcgttg tacttttagg
    23401 aacatatcca tctttacgaa ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag
    23461 acaagctttg aaaaaagtag ggatggaaga atatgccaaa agacagatca gcgaactatc
    23521 gggtggacag cttcagagag tttttattgc aagagctcta gcccaaggag cagaatggat
    23581 ctttttagat gaaccattcg tagggattga tgcgttaagt gaacgaaaga tctttgacat
    23641 cttgcaggaa ttgaagaatt caggaaaaac gattttgatc gtccatcatg atcttcataa
    23701 agtagaagaa tatttcgatg aggttattct tttaaataaa cagctgatcg cttccggtcc
    23761 agtacaagaa gtctttacat cgaagaacct tcaattggcc tatggtgaga tcatcagaca
    23821 tttagtaaaa ggaggagaga aaaaatgatc caatccttta ttgatggatt aatgaattat
    23881 caattcctgc aatacgcttt ggtcacttcc atgcttgtag gacttgcttc tggagtcatc
    23941 ggttctttta ttattttacg agggatgtct ttgatgggag acgctatctc tcacgcagtt
    24001 cttccaggtg tagccatttc ttatatgttt ggttttagtt acatctttgg agccactgct
    24061 tttgggatgc taacagcagc agtgattggt ttcgtgactc agcggagccg attgaaaaat
    24121 gatacagcga ttggaattgt ctttagctcc ttttttgcat tagggattat cttgatttca
    24181 tatgcccaga gtgcgacaga tttgtatcat attttatttg ggaatgtctt agctgttagg
    24241 gaaagcgatt tgttgctgac agcactcgtt agtggaatcg tcttgatatt tgtctttttc
    24301 ttttataaag aattgaagat cacatcattc gatccgacaa tggcaaaagc ttatgggctg
    24361 aacacatcgc tgatccatta tcttttgatg ttctttttga cattagtggc tgtagtcagt
    24421 ttacagacag taggaacaat cttggtgatt gccatgttga tcacaccagc cgccacggct
    24481 tacttgctaa cgaaccattt actgaaaatg atcattacag ctgcaggaat cggtatgcta
    24541 agtgcagttg tcggtgtgtt tttcagttat agttacaatt ggccatcagg agctacgatc
    24601 gtgttagcat gtaccgcatt ttttatcctt gcttttttat tttctccaac aaaaggaatt
    24661 ttatttcaga aaggaagcag atagatgaaa acaaaaaaat cactcttttt aatcttggca
    24721 gttagtttct tggtattagc cggttgcgga aagcaggctt ctgaccaagc ggatgaagga
    24781 agtaaagaaa agttgtcagt cgtggctacc aattcgatct tggcggacat ggcaaaagag
    24841 gtcggaacag atcaaataga tatccacagt atcgtaccag tcggaacaga tccgcatgaa
    24901 tatgaagtac taccagaaga catcaaaaag gcaagtgatg cagatgttat tttatacaac
    24961 ggtttgaatc ttgaaacagg taacagctgg ttcgataact tgatggaaac ggctaaaaaa
    25021 gaagaaggaa aagattattt tgcagttagc aaaaatgtag aacctcttta tttaactagc
    25081 ggtgaagaac atacaaaagc agatccccat gcttggctag acctatctaa cggaataaaa
    25141 tatgtggagg aaatcgcacg tatattctct gaaaaagatg cagaaaatgc gacactctat
    25201 aaaaaaaatg cagaagcata tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa
    25261 agttttgctt ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag
    25321 tatttttcac gagcatatga tttgccagca gcttatatat gggagatcaa tacagaaagt
    25381 caaggtacgc ctgatcaaat gaaagcgatc attgatcaga taagagcaaa agaagtaccg
    25441 gttttattcg tggaaaccag tgtagactca agaagcatgg aacgggtagc aaaagaaacg
    25501 ggtttgaaaa tctatgataa actgttcact gattccatag caaaagaagg agaacaagga
    25561 gattcttact atcagatgat gaaatggaat attgaaacga tccatgaagg tttgtcacaa
    25621 acaaaagaaa gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc attttttata
    25681 agagatatga aaaaaaatta tgctgttttt cgtcgtacag ataccttttt tgaagaaatt
    25741 ccgttatagt tagagtaagg aaggaaggtg tggctatgaa ttttacacgt aaagataaat
    25801 atacgattaa agaagaagtc tttcgattga tcagccagca ggaaaatacc gacaaggatc
    25861 aagaccttca atttcttgaa gcagaattag ctaaattatt catagcaaat caaaatgaga
    25921 caacagaacc agatgaagag ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt
    25981 tagatgaaaa taccggtatc ggttattttg tgttcctttc tccagtcggc ttcacacctg
    26041 gcgtattagt taaacaaaac gacgaaataa ccgtcaaagc atggcctgca ttattttcag
    26101 tcatcaaagc agaaggagta atcaaaaaga tccagtcggc ctattataaa agtcaattta
    26161 ttgccaacag caaattaatg aatatgtttt ataatcgtct agtagaaatt gctaggcctg
    26221 ggaaaatccc gcaaacattc acgtacacac agcaaaaaaa tctctatgaa gaagtattgt
    26281 attcccgcga tctgtctgag ttttcgccat gggaagaggt aaggctccat gaactgttaa
    26341 gtacaaaacc agtttcattg aattttttta atgcattaga taacaaaaga aaattaattt
    26401 caacaaatta ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg
    26461 ccgatcagca gatccagttt gtccagctga aaaaagaaaa actatattat gggttataca
    26521 gccgttaaca gcaagcgagg aagaccatga cattaaaaga agaaatcata caagaaagca
    26581 aacgattagg aattgacaaa atcgggttca ccacggcgga accttttgat tcattaaaag
    26641 aatcgttgga agaacaaaaa gcagctggcc atacttctgg ctttgaacat ccaaatatcg
    26701 atgagcgtct ttatccagaa atgacatttg atcagccgaa atcgatcatt tccattgcac
    26761 ttgcgtatcc tactaggatc catgaagaag tgcctaggga tgaaaaacgc gggcagtttg
    26821 ctcgtgcttc ttgggggatc gattaccatg atatactcag agaccgtttg aaccgattga
    26881 tcgaattcat ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc
    26941 cgcaagtaga tactggagaa ctgatcgatg tcgctgtagc acagcgagct ggtttaggat
    27001 ttattggacg aaacggattg ttgatcactg aagagttcgg ttcatttgtc tatcttggtg
    27061 agatcgttac taatattgta tttgagcctg ataaaccagg agaatttggt tgcggtgact
    27121 gtatgcgctg cgtgactgct tgtccgacaa aagcattgct tggtgacgga agaatgaatg
    27181 cccagcgatg tttgtcttat caaactcaga caaaagggat gatgccggaa gaatatcgta
    27241 aaaaaatgcg aaatgtcatt tatggatgtg atatctgcca gttagtctgc ccttacaatc
    27301 aaggaaaaga ttttcatttt catgaagaga tggaaccaaa agtagacgaa gtatatccaa
    27361 aattaaagcc aatgctttcg atgtctaata aagacttcaa acagcaattt gggcatttag
    27421 ctggttcatg gcgtgggaag aaacctttgc agagaaacgc attgattgct ttagctaatc
    27481 taggtgatcg aacggcattg cctgagattt cagaatgttt aacagatgtc cgccctgtca
    27541 tcagaggaac agcagcttgg gctattggac gtctaggaac aaaagaaccg gaaaaatggc
    27601 ttgatgtatt agatgaaata ttagctaaag aaacagaaga agtcgttata aacgaattgg
    27661 aacatgccca gaaattattg cggagaaagt taaaataaaa aaatagttaa cattattcaa
    27721 agttt
//
LOCUS       DAHHPA010000028        27518 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.029, whole genome shotgun sequence.
ACCESSION   DAHHPA010000028 DAHHPA010000000
VERSION     DAHHPA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.029"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            244..1722
                     /locus_tag="NBH55_001126"
     CDS             244..1722
                     /locus_tag="NBH55_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1509123.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            1999..4059
                     /locus_tag="NBH55_001127"
     CDS             1999..4059
                     /locus_tag="NBH55_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCD1509124.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4142..4711
                     /locus_tag="NBH55_001128"
     CDS             4142..4711
                     /locus_tag="NBH55_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1509125.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4886..5692)
                     /locus_tag="NBH55_001129"
     CDS             complement(4886..5692)
                     /locus_tag="NBH55_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509126.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFMFIKNFIPKKIKR"
     gene            5830..7344
                     /locus_tag="NBH55_001130"
     CDS             5830..7344
                     /locus_tag="NBH55_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCD1509127.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7747..8730)
                     /locus_tag="NBH55_001131"
     CDS             complement(7747..8730)
                     /locus_tag="NBH55_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCD1509128.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8735..9382)
                     /locus_tag="NBH55_001132"
     CDS             complement(8735..9382)
                     /locus_tag="NBH55_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1509129.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9467..10099)
                     /locus_tag="NBH55_001133"
     CDS             complement(9467..10099)
                     /locus_tag="NBH55_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1509130.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10115..10666)
                     /gene="coaC"
                     /locus_tag="NBH55_001134"
     CDS             complement(10115..10666)
                     /gene="coaC"
                     /locus_tag="NBH55_001134"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCD1509131.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10663..11400)
                     /locus_tag="NBH55_001135"
     CDS             complement(10663..11400)
                     /locus_tag="NBH55_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCD1509132.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11397..12386)
                     /locus_tag="NBH55_001136"
     CDS             complement(11397..12386)
                     /locus_tag="NBH55_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1509133.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12644..13186
                     /locus_tag="NBH55_001137"
     CDS             12644..13186
                     /locus_tag="NBH55_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1509134.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13203..14288
                     /locus_tag="NBH55_001138"
     CDS             13203..14288
                     /locus_tag="NBH55_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509135.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14300..15106
                     /locus_tag="NBH55_001139"
     CDS             14300..15106
                     /locus_tag="NBH55_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509136.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15107..15934
                     /locus_tag="NBH55_001140"
     CDS             15107..15934
                     /locus_tag="NBH55_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509137.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15931..17004
                     /locus_tag="NBH55_001141"
     CDS             15931..17004
                     /locus_tag="NBH55_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1509138.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17432..20128
                     /locus_tag="NBH55_001142"
     CDS             17432..20128
                     /locus_tag="NBH55_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCD1509139.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            20205..20693
                     /locus_tag="NBH55_001143"
     CDS             20205..20693
                     /locus_tag="NBH55_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1509140.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            20705..21199
                     /locus_tag="NBH55_001144"
     CDS             20705..21199
                     /locus_tag="NBH55_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1509141.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21220..22029
                     /locus_tag="NBH55_001145"
     CDS             21220..22029
                     /locus_tag="NBH55_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1509142.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            22026..22847
                     /locus_tag="NBH55_001146"
     CDS             22026..22847
                     /locus_tag="NBH55_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCD1509143.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22898..24997
                     /locus_tag="NBH55_001147"
     CDS             22898..24997
                     /locus_tag="NBH55_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCD1509144.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            24994..26112
                     /locus_tag="NBH55_001148"
     CDS             24994..26112
                     /locus_tag="NBH55_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCD1509145.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26151..27029
                     /locus_tag="NBH55_001149"
     CDS             26151..27029
                     /locus_tag="NBH55_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD1509146.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27220..27372
                     /locus_tag="NBH55_001150"
     CDS             27220..27372
                     /locus_tag="NBH55_001150"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCD1509147.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8809 a         4711 c         5742 g         8256 t
ORIGIN      
        1 tttttgtggt tgtttacgca aatggcgcaa atcttgtaca ttttaacaca ataaaacaaa
       61 aaatatagaa tatttcctca taaatttttc cattttttgc aatattctca aaaaaaagat
      121 aaaaaatgag aataactgat gaaaaaacaa gcataatcca tcataaaatc agtataggtg
      181 aaaagatgaa ctttcatctg catatcaagt aattggtatg aggcattttt tgaggtgtag
      241 gatatgaagt tggattacct tatagaagag gaagatagag aaaaactgct gctagctaga
      301 tatttagaat ttgagaaaaa gaattatgta gtgctgccta agttatatga aacattaggt
      361 ttgtctaaat ttaaaacaag aaattatatc atagaattaa ataaagaatt tactgaattc
      421 aaaacaaagc cagagatcca aatttcagaa gaagcagaga tccatgtcga acgtctgaat
      481 ttgacggata tcaaaaattt aagaagaatc tactttcaac gatcaaaaat tgctcgactg
      541 ttaacggaga tcgtggattc aaaagtaacg atcgaaaaat ttgcagagaa tcatttttta
      601 agcatatcaa gagcctatgt aaaaagaaga gaattagtct cttttcttga acagcatcaa
      661 ataaagttaa agaaaaacat attagttggg gaagaaataa acatacggaa cgtattattt
      721 ggggtatatt tcggggtctt taatggtttt tctatgccat ttagagaaag cgtcatgaat
      781 gaagcaaaga gtatcgtgca atattttgaa tatctgttca atttaaaact gtctgcaaca
      841 aaacgaatca aattcaattt gctaaccact attagtttgt tgagggccta tgggaatcaa
      901 ccaatcggaa aacgtttctt ttcagatgaa tactttaaga gctcagaagg aatgaagatc
      961 atcaaggatt tatcacatat ttcttttctt catatcccta aagagcagat cattgatgag
     1021 atttcttata tcatgttgtt tttgatcacg gaagaagaat ttgaattaga tggcgcattt
     1081 ggacttgcgg aagctcattt tcatgatatc cacatgatta gtcaaaaaat gtcagtggaa
     1141 ttgattgatc aattaccatt ttcgattgaa atctctgaag aagaaagact atatatacga
     1201 aaagaacttc gtatgggtat gatgcatgtc aatcgaaaaa attggatatt cgactttgag
     1261 atgactagct ttacgtctaa aaagcagcta cagtttttct tcgagacgta tccaatattc
     1321 agtacagcaa tccaaaaggt catacaagag aataaacgct atttcttggg aaaagatgaa
     1381 cgtttcatga ttcgcttatt ttatgattat ctctttttat tggtcggtat ttgtccggta
     1441 tcatattttg aagcaccgat tcatgtctgt attgattttt cgcaaggatc tgcttatacc
     1501 gattacatta tttctcaagt gaaaggattt aaaaattaca atattgtgat tgagagtcga
     1561 atcacttctc gtacgaatat ttatttatct gattgtttac ttgaaaaatt taataaagaa
     1621 cagataattt ggaaaaatcc accgactcca gatgactggg aatattttgg aaataccatc
     1681 atcagagaaa aaaatagaca tttaactgaa aaaatcagct agtatttatg gatggcagct
     1741 ttgtctcaca tatttattag ctgatttatg tttttttcaa agaaaaggta actagatata
     1801 actagatata actagatata ttacttaaca aaattaaaaa gtacataagt tattattccg
     1861 attttcttga aacaataaaa aatcaaaaaa aatactctcc taatagtcca tcttcttttg
     1921 attttattta tataatgaaa tcatcttatg aaaattacta cttcatgttt agacaagagt
     1981 agggaggact aagaataaat gtgttcaaaa agacgttcaa aacaactaaa taaacacgct
     2041 tgtcgtttag ataaggaacg gtcgtgttct gaaaaacact acgttaagtt tatgatgcgc
     2101 acgatgctga tcagtttatc tgtaggtgtc tatatgcatg tacccgttca ggcaacgaca
     2161 caaaccatca aggtcgtacg tacctctgtt tctgagacca ctgattcccc tatcactatt
     2221 actcatactt tcactgatgc aaatggaaaa cagctacttc ctgcattgac ttcctataat
     2281 aaagtcagtt atcctccaac gataaaccac tactggttaa ttcctaacaa aacattatgg
     2341 acatcggaaa ccggaagcag tgtgccgctt tctatctttt ccgatatgac tgatcctaca
     2401 aaaggtgatc agttaaaaca gatcactgat attttgaatc atgctttgac agatataaag
     2461 gacggtggaa gcgcacaagt ttcttatgta tatgaaaaag attattcaca gtttgaaaga
     2521 aagaaaatcg atgttctggc agggacaaaa gtttcgatat ctgatctggt agaaaggctg
     2581 aagaacgcag atggaacaca aggagatatg tccaaagtaa tttcaaacaa tgggaatatc
     2641 gtggatacga ctaatacagg aacatctact atttccttgt cctattttga tccaattgct
     2701 ttaaaaacaa tgactgcatc tgctgtgatc catgttcgtg ctgatcaaac agctatcaaa
     2761 ggaaaagatc ttcacttaag aacaggagag agttacgccc ctaaagatct tgttgaatat
     2821 gtaacaaaaa gcgatggaac ttcaggaaat aaagaagaaa tcaaaatgaa cgggacagta
     2881 gatactacaa ctccgggaga atatcccgtt gtattaactt atatagactc gaaaacatca
     2941 aatgtggcag aaacaacagc tgtagtttat gtagaagcat ctgctgcttc atcgactgtc
     3001 atcactcatt ttatagatag tcaaacaaat aaagaacttc aagcagaaac acatatgagt
     3061 agtgtaaatc gtattcctga tttgcgccta cctgctgaat tttctgatcg agtgccaaat
     3121 atagatttat cgacttatga acaaaatggg aaagtcactt ctatttctga ttggatgcaa
     3181 gcaaacggat tagacagaaa aagtcactgg tcggagatcc ttgaagtact tcactcagaa
     3241 ctgatgaata ataggaagca atcaggaggg gaaattacct ttacttatgt ttatatacca
     3301 gacaatcgtt cattagaagg tcatgatctt attgttcatc caaacgaaaa aataaacaaa
     3361 gaagagttga tccaatcagc aacaaattcc ttaggtaaac ccgtaccgtt aaaggatgta
     3421 tcggttacaa tcaatgaagt accattggag gatggttatt tgaccaaaca aacaggtgat
     3481 tttacgatta tgtatagtta tcatgacccg tattcactcc aagaactcca tgctcagtca
     3541 ctattacatg ttttaccatt aaacaaacag atagatcaaa aagaacaaaa aagacaagaa
     3601 gaaagcttgt cagaaaaaaa acaacaaatg gctcatgcag tttctccaac agggaaagta
     3661 aaacaaacaa gaagcctaga gttagcaaga atatctagaa aaatctcaga atctaataaa
     3721 acgataaggc aagaagctca tttggcccca caagtttctg gattggaaaa tgaaatttta
     3781 gagcctaaaa taaaacaaca atcagaaaaa gaatttgttg ctaaacagtc ggaagatcag
     3841 aaggaatcag atgaccagaa ggaaacgaag aaaaaacgct tgtcaactat tgattccacc
     3901 gatgagcatc tgcagccgca agtaccactt cctagtccgg gaacacccgc acctggtgga
     3961 ggtattcctt cttctgcttt aggagatttc atgcgaggta tggcaggaac atgggtatac
     4021 gctaatcgaa atgaatttga cgcagatgac tggttctgat ttatatctct atctgttata
     4081 gtcaaataag tagctgagtc atatgttatt tggcataatg gagacgagga gggatagaaa
     4141 aatgttagat aaaacgttac cttacgcaga aatctggatg acaagaccac ttcaacaaaa
     4201 tgttccaatc acgcctttag cggaaggttt tgaatttcga ttttatcaaa aagaagatga
     4261 atctgcttgg gcggagattg agacttccgt tggggaattt caaacgacag atgaagcact
     4321 tgagtatttt caacaaaact ttgctcctta tcctgaagaa cttgaaagaa ggatgttttt
     4381 tgtagagaca aaaaaaggag aaaaagtagc aacctgtacc gcttggcgaa aaaaatcaag
     4441 agagcaagtg tatccggttt ttcattggtt agcagtgaaa ccagattacc aaggaaaagg
     4501 gctcgcaaaa gcattgactg ccagagtctt gcaagaattt cctaccctcc atacggacgg
     4561 accaatctat cttcacacac agacatggag ccatcaggcg attgctcttt ataaaaaaat
     4621 gggttttact tttattgctg aaaatctaga cggcacacct aatccagatt atgaaaaagt
     4681 tatgcaggtt ttaagtaaat tagataaata acgaaatggt gaaataagtc ctcagtattg
     4741 ggataaaaaa cgaaatagtc attcctcata tcttactttt ttgagttaat actatcggat
     4801 aactaatggc atgccataca ttcaaaaaag aaggggcagt gacaaaagta ttgtcactgc
     4861 cccttctctt gttttttagc atgttttatc gtttgatttt tttaggaata aaattcttga
     4921 tgaacataaa tttttggtaa tcgtttcgac tatagaggta accaaataca ctgaaaacaa
     4981 ctgctccaat aaaattaaca aataaatcct tcatagtatc catgattccc acgtctaagt
     5041 aaccattttg gatgattgtt ttatctgttt taccatctgc attttgactt tcaatgattg
     5101 tccgagtgat atctggaaca tgatagacag tgtttccgtc tttacttaac tccacactat
     5161 tgatttgctg gatgatcgta tctttttgca tatcgaattg cagtaattta tctgctgaaa
     5221 attccacaaa ctcccacata acaccaactg tcatcgaaaa acagaaggaa acaactgcta
     5281 gtaataaagg agtcaagttc atggtttttg tatttttatt gagcaaataa acaagggaaa
     5341 agccgacacc cccagctaaa aatccgctga atgcatgtag aaaagtatcc cacattgaaa
     5401 aatagccata aaaatcactt aactctccta aaactgttga actaaaaata aaagataaca
     5461 aaatgatttc tagcatattt ggaatctgta tctttaatat cttttccaga aaataaggga
     5521 tttcaaataa aattaatgtc agtaataaaa gcattgtatt tccccagtgt tggttccaga
     5581 cctgatccac tagcataata acgacgatga tacgtaataa aatatagatt ccgtttgtga
     5641 ttctacctgc agtttttgta ctgttcgtaa aaaaggtttt caaatgattc ataaaataac
     5701 tccttcatgt atatccttaa ttatactaag aagcgtgtgt ttaccaaagc tatatgactt
     5761 aaagaaaaac agagttgata ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg
     5821 gggatacaaa tgaaaaaatt agttgctggt ttattaacag ccgttgcact tcttgctatg
     5881 agcgcatgcg gcgcagatca gaaaaataca gcgcagacaa gttctagtac gaaggaatct
     5941 gccgaagtat catcaggcgc ttctgaacaa gtatatacag atccaaaaga aatgaaagaa
     6001 tcttatgatg tcgtgattat tggagctggt ggtgcaggga tggcagcagc tatcgaagca
     6061 aaagatgctg ggttgaatcc agtaattttg gaaaagatgc ctgttgccgg aggaaacaca
     6121 tctaaatcat caagcggtat gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc
     6181 aaagactcga atgatgcctt ctatgaagaa acgctaaaag gtggtggagg aacgaatgat
     6241 aaagaaatgc ttcgatactt tgtcgatcac tctgctgatg cggtcgattg gctagatcaa
     6301 aacggtatca aactggacaa cttaacgatc actggaggga tgagtgaaaa gaggacacat
     6361 cgtccgtctg atggctcggc tattggtgga tacttagtgg aggggttatt gaataacgtc
     6421 catgagcggg aaatccctat ttttgtcaac gcagatgtga cagacattaa gaaaaatggg
     6481 gaaacggtca atgaagtgac tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga
     6541 aaagcaatcg tagttaccac aggcggtttt ggtgccagca aagaatttat tgaaaaatat
     6601 cgtcccgatt tagctgatta tattacgaca aatcaagaag gatcaactgg agatggtatc
     6661 accatgattg aaaaattagg tggacaaacc gttgatatgg ataaaataca aattcatcca
     6721 actgtgcagc aggatgaggg ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc
     6781 ttggtcgatc aagaggggaa aagatttgtc aatgaaatgg atacaaggga taaagtatct
     6841 gcagcaatta cagcattgcc tgaaaaatca gcttatttga tttttgacca aggcgtgcgt
     6901 gatcgtgcca cagctattga attttatgac caaaaaggat atgtcacaac tggaaagaca
     6961 atggaagaat tagcagaaaa aatcgattta ccaaaagaaa cactagcaaa aacagttgat
     7021 ggatggaata cggatgttgc ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg
     7081 gaacatgact tatcgtctcc gaattattat gcgatcaaaa tcgcaccagg aatccattac
     7141 acgatgggcg gtgtgaaaat caatacgaac actgaagtat tagataaaga aaaccagcca
     7201 attagggggt tatatgctgc tggtgaagtc acaggtggct tacatggaaa caaccggatc
     7261 ggtggaaatt cagttgcgga gatcattatt tttgggcgac aagcgggcat acaagcagct
     7321 cgatttgctt cggaaaatga atgaaataga acgtaaataa aaagaggttg tgaaatcagc
     7381 gttttgagct aataccgagg gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa
     7441 aagctgccct gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttaa
     7501 cttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttattc ggaaaaagag
     7561 cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa
     7621 gcagaagaaa ttccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg
     7681 tgaatttctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttgatcagc
     7741 atgattttat tcagttatct tcaaaatgat ttttccgatg tttttgtttg cttccatata
     7801 ttcatgcgct tgtttgactg cgctgaattg gaatgtacgg tcaatgattg gccgaatctt
     7861 gccattttct agaaaaggcc cagctttttg cataaaagtt tgtgcgagct ctgctttata
     7921 ctgatcactt ctaggtgtca ataatgtacc aagcagttgg acacgtttcc caatcaatac
     7981 accaaggtct atctcaggca cgatggtccc acctagcata ccaatcaaga cccaacgtcc
     8041 atcaacggct atagaagcta aattcttttc ccagtaagaa gcgccgatga aatccaaaat
     8101 aacatcgact ccccgacctt cagtgatttt tcgtacctct tcttgaaagt cttgtttttt
     8161 atagttgatc aattcatctg caccaagttc tttacaaaag gcgagttttt cttctgatcc
     8221 tgcagtaacg atgattttcg cgtgagaaag ctgtctagct aattggatcg ctgctgttcc
     8281 cacaccactt gctcctgcat ggatcaagac agtttcgcct tcttgtaatt ttccaagcca
     8341 gtaaagtgtt tgataagcag tcaaaaaaac ttctgagatt ccagcagcag aaacatagtc
     8401 tagcgtatca gggagaagga ttgcacgatc ggcaggcatc acagcatatt cggcataacc
     8461 acctagattg actaatccgt atacttttgt cccaggagta aaagtggaga tatcactttt
     8521 attctcaact acctctccag ctacttcgac tcccaaaata gggtagggag gtttcaaaga
     8581 gactgattct cttttcataa tatctgttcg attgacagct gctgtatgga ctttgatcag
     8641 taattcgcct tgttttggtt taggaacagg cacttcaatt tgttctaata catctacaga
     8701 accaggatgc ttgattgaaa tggcacgcat ggatctattc ctcctttgcc cattcttgga
     8761 atgctgtatt caactctttg ctaccttcag aaagtactag agtcgtacga gaaatatccg
     8821 tttcttccac tagagcggca agtacagcag caactgtaga gcgagtgatc gtttttgatg
     8881 ccttctcatt aggcttagtt aattgaatgg aagtaacttc ttcatcgtta gtcaatgtga
     8941 ctggttgaag aatgacaaaa tctaagttag ttcgtttcag ccattcatct gcataatgtt
     9001 tagtaatata ataatctgtc atggactcag gccattttgc tggttcatct gcataaacag
     9061 cactgaccat cagatagcga gagacatgcg cattttcagc tgcgatcact gttttgattg
     9121 cgccatccaa gtctacttgc agaagattct ttccttgaga gccggcagca aaaatcaata
     9181 catcgatgcc tttgaatgct tcggtcattt tttccacgga ccaagttaaa tcaaaagaga
     9241 catacgaaac attctctttt tttactaatg tttgggaagc aacatctctc acccctgcaa
     9301 aaatttcatg ggaacttgga gctaaatctt ttatcaggtg tcggccaatt tgtccgtttg
     9361 cacctacaac aaaaattttc atttggatca tcctttcgac gtcaaatctt tccattcaat
     9421 agtaacacca aaagagaaaa aaatcagaga tcggctacga tgactatcag ttagtaagag
     9481 aagggtgctt gaagtatctt aacaagacat tgcatactgc tgcagtcgca agacctgcga
     9541 cgattccttc aggtatacca cttgtaaaaa taaccgctag aatagctgaa taaacagcat
     9601 ttaaatttgt tccgatgact ttggcataat cttgctggaa taaaaagtag atcatattca
     9661 tcactaaaat cgtattggtc aatgaaccgg aaagtccagc taagaataaa gaaacggaat
     9721 ctgctttttg tttcattagt ttgcgcagac ctttataaac gaaataagga accaccccaa
     9781 tcaagatgcg aggaataaac gcgatcagca aggctttcca gcttccatgt tctgttccga
     9841 tgactgggat aaacggagaa aagacaaaag acaaaggtgt ttggataact gtgctacgga
     9901 tcatgctgat cagtccaaat gtaccgccaa gaaaagcacc tagtcttggt cctaaaacga
     9961 tcgaagcaat gattacagga atatgcatgg ttgttgcatt gattggaccg atagggataa
    10021 aacctaaagg cgtaacagct aataaaatca aaatagctaa aaacatcgca gttaatgtaa
    10081 agttttttgt atttttcata agtactcctt ttttctaagc atttctttca ttaagagtca
    10141 gcatgacctg ttcgatgatc gtctgaatat cagctaaggc accttttcca aaatctccac
    10201 aagctaaaag agattctctt ggttcaattt cctgatagcc gacagatctc aatgtttcca
    10261 gattacgctg agtgatcggg ttttgataca tattcgtgtt catggcaggg gcaatcaatt
    10321 ttggtgttcc cactggaagc gccattgcga ctgttgaaag aagatcgtct gcaatgccgt
    10381 ttgctagctt gccgataatg tttgctgtag caggcgcgat gagaaacaag tcagcttgtt
    10441 ttgctagttc gatatgattg attacctgcg gatttgattc ttgcatgacg tctgtatgga
    10501 ctgcgcgctt cgacagagat tgcaaggtta atggcgtgat aaaatgtgtg ctgcttttag
    10561 acataatcac atctacctgg gcacctaatt tgacgaattg acttgtaaga tcagcgcttt
    10621 tgtaagcaga aatacttcca gtaacgccta gtaagatctt tttcattttg attcctccag
    10681 tctaattttc tcagtaatca atgcagcaat ctcagattta ctttgagctt cttccactgt
    10741 tccatcttta gataagagat agccgtggtg tgtctcgcca tggatttccg tcaaatcatt
    10801 tgcgaaaata aaatcagttt tgttatttat cagactattt tttgcgactg ccagaagaga
    10861 ttcctttggt acatctacta atagtttgaa accaatgatt tttgcttttg gctgccattc
    10921 acgtagataa gaaatgattt ttggtgtttt cttcaaaacg agcacaagat gctcggtgtt
    10981 agaagagatc tttgaaacag tggtatctgt ctgttcattt tccgcaaacc aattgtccag
    11041 ttcttcttgc gttgaagaag tgggtaggtt ttttgccagc tgttcttctg aaaaactaaa
    11101 agcaggagta aaatcactga ctgccatact gtggataatc gcatcgtagt gttctttttt
    11161 cgttagttct tctaattgta aaaataagtc cagtgcagat tcgatcgtat aaatcgttat
    11221 attggagtgt cttttcggtt tcagtgcttg tttcgtagtc acgtaatcga ttgtggtatc
    11281 cggattggca gctaaataat cagctaatgc ttgccctaat cgtcctgtcg aatgatttgt
    11341 aatagcacgt acttgatcga ttttttcagt cgttcctcca gctgtgatta gaatcttcat
    11401 ttatggtcac ctacatttac tagtgttctt ccttggtgtg ttccagcaag tagtttagat
    11461 gctgtttcag gcaattgatc cagagtaatc tcttggacga ccagttcatc tgcaatctgc
    11521 aaatccgcca tctgttgcca taaagaaaga cgttgatcta ttggtacatt gacagaatca
    11581 attccaatca actggatgtt tcgcagaata aaaggcaaga cagtcgcgtt tagtttgatt
    11641 cccccagcat tgccacataa agctactgca ccgttataag agatcaatgg aagaagactg
    11701 cttagctgct cgccacctac tgtgtcgatg acatagtcga tttgctgttt tcctaaagct
    11761 tttgttgtct ctggcagaaa ttcatcaggt cgtacgactt gcaaagcacc tagacttttt
    11821 agccatgtga cagactcttc ttttctggaa aaggcaatga ttgactggaa tcctagttta
    11881 tgtaacaggg ctatcgctgt actgcctaca ccgccagaag caccagtaac gacaactttt
    11941 ttatcggagg tcataccttg acggattaaa gcgttcacac acaacatagc agtaaaccct
    12001 gctgtaccaa ggatcatcgc ttgacgcagc gtcatatttt ttggtagagg aactaaccag
    12061 tctcctggta ctttttgata ttggctataa ccgcctggat gggtgacgcc aagcccaaaa
    12121 ccagtagcaa gtacaagatc ttcttttgaa aaacgtggat tgttactttg gacgacagtt
    12181 ccggataaat caatccctgg agtcatcgga tatgttcgta tcacctgacc agactcgctc
    12241 attgccaatg catctttata attgacatca gaataggcaa tttttaccaa gacttcattt
    12301 tcagccaact cagtaaatgg cagatcactg attgctgtat ggaaaacagg ttcctttatt
    12361 acctgaaaag atcgaaaagc gttcattcgt gagacactcc ttttaaaatt ttcttttatc
    12421 ataccataaa gcctactctt ttattcttgt tcttccgtaa atttagacaa aaattaggtg
    12481 aaaaataaag aatcaaacaa ctagaattta ttccaaaaga taaagaaata ttcagcaaaa
    12541 aaaattttac aaagggttgc ctttttcaat aaatttcgta tactaatacg agtggtagta
    12601 aacaggaagt ttactattag aaaacttcga ttcggaggaa attatggaaa ttggtgagaa
    12661 gttaagaaat ttgcgcatcc aaaagaattt gacacaagaa gaactaggcg aacgcacaga
    12721 tttgtcaaaa gggtatattt cccagttgga acgagattta agttcaccat caatggaaac
    12781 attcttttct attcttgaag tcttaggtgt gactccagaa gaattttttc atcaagaagc
    12841 agcgaatctg caagtggttt attcaaaaga agatcatacg gtttatttcg atgaagaaaa
    12901 cgggtatgaa ttggaatggc tcgtggctga ttcgaatgaa aaagaaatgg agcctgtctt
    12961 gctgactttt gaaggtgcag gagaatataa gacgtttgaa ccttcattat ctgaaacgtt
    13021 tatttttgta ttagaagggg aattagagtt aagcttagga gagacggtct atgtggcaaa
    13081 agcaggacag tcgatctatt atcaagcgac aaaacaacat caattaagga atcatctcaa
    13141 aaaacgaaca aaagtattga ttgttgcaac gcaatcatat ttgtaatata gggggtaaaa
    13201 acgtggggaa accaatcatt tcatttgatc aagtagtgaa acgctatgat gatgaaacag
    13261 tattaaaaaa agtaagtttt gagatcgaac aaggaaaatt ttatacactg cttggtcctt
    13321 ccggctgtgg taaaacaacg atcctacgta ttatagctgg attcaccgaa gctacagaag
    13381 gggacatcta ttttgaaggg aaacggttga atgatttgcc agctaataag cgacaagtaa
    13441 atacagtctt ccaagattat gcactttttc ctcatatgaa tgtttttgat aacgttgctt
    13501 ttggattgaa gatcaagaaa atgtccaaat cagatatcga aaagaaggta aaagatgctt
    13561 tgcgtatggt ccagcttcct ggatacgaaa aacgggagat tagtgaaatg tctggtggtc
    13621 aaagacagcg tgtagcgatt gcacgagcaa ttgtaaatga accgaaagta ttgctattag
    13681 atgaaccgtt atcggcgctt gatttgaaac tacgtacaga tatgcagtat gaactgcggg
    13741 atctgcaaca acgattagga atcacgttta tttttgttac tcatgaccaa gaagaagcgt
    13801 tggcaatgag tgatgagatt tttgtcatga ataaagggaa aatcgtccaa agtggtacac
    13861 ccgtagatat ttatgatgag ccaatcaatc atttcgttgc tgattttgtt ggggagagca
    13921 atatcgtgga cggtgtaatg atcgaagata atctagttga gtttgttggt aagcaatttg
    13981 aatgtgtcga tggcgggatg cgtctaaatg aaccagttga agttgtttta cgcccagagg
    14041 atttgacgat cacgacacca gataaaggaa aactagtcgt aacggtcgat actcagctgt
    14101 ttcgtggtgt ccattatgaa atcatctgtt atgatgaaca gcaaaatgaa tggatggtcc
    14161 attcaacgaa aaaagctaaa gaaggaagca aagtaggatt ggcctttgag ccagaagata
    14221 tccatgtcat gcgtttcaac gagtctgaag aagaattcga tgcacgtctt gacagctacg
    14281 aagaataggg gggacaagaa tgaaaacaat gcgcaggatc tattccgtcc cttatatttt
    14341 gtggctcggt ttattcgtga tcgcgcctgt attaatgatc atttatcaat ctttttttga
    14401 tatgaatggg caattcacgc tgaataatta tctatcttat tttacgtcgg ggacatcgct
    14461 ttctatgacg atcaattctg tatggtatgc atttttgatc acgtttttca cgctgatcat
    14521 cagttacccg actgcttatt ttttaacaaa actgaagcac aagcaattat ggctgatgct
    14581 gattattttg cctacatggg tgaatctgct gcttaaagct tacgcattta ttgggatttt
    14641 cagtgtgcac ggcagcgtga atcatttctt tgattttatc gggatcgggc cgaaacagat
    14701 attattcaca gacttcagtt ttctgttcgt ggcaacgtat atcgagattc catttatgat
    14761 tatgccaatt ttcaatgcat tggaagagat gaatccttct ttgatcagtg ctagtagaga
    14821 tttaggcgcg aatagctttg agaccttccg acgagtgatt tttccgttat cattaaatgg
    14881 tgtgaaaagt ggtgtccaag cagtatttat tccttctctt tctttattca tgctgactcg
    14941 tctgattggc ggaaatcggg tgatcacttt aggaacggct atcgaacagc atttcttggt
    15001 tacacaaaac tgggggatgg gttctacaat cggtgtgatt ttaatcattg ctatgttcat
    15061 cgtgatgctc ctgacaggcg agaagaaaaa aggaggtcgc tcataaatga aaaaatttaa
    15121 atggtcgtat ctttatttag ggatcgtctt tttattctta tatgcaccga tcttctatct
    15181 gattttttat tccttcaatg aaggaggaac gatgaacagc tttacaggat ttacttggga
    15241 aaattatgaa gctgtttttg aagatacacg tttgatcatt atcgtattga atacgttcat
    15301 gctggctttc ttatctgcat tgatcgccac gatcatcggg acatttgggg ctatgggaat
    15361 ctattacacg aaaaaacgac gtgcacggac agcgttgttg agcatgaata acatcttatt
    15421 agtctctcct gatgttatta tcggtgctag tttcttgatt ttctttacac tactaggatt
    15481 cggattaggt tttcagagtg tattgctttc ccatattgct ttcagcattc caatcgtcgt
    15541 cttgatggtc ttgccaaaat tgcaggaaat gaacgattca atggtcgatg cggctagaga
    15601 ccttggtgct aataatttcc aagtagtcaa aaatatcatt ttgccttatc tttctccagg
    15661 aatcatagct ggatatttta tggcatttac ctattctctt gatgattttg cggttacatt
    15721 tttcgtcaca ggaaatggct ttacgacgct ctcagtagag atctattctc gtgcaagaca
    15781 aggaatcagt ttagaaatca atgcgctaag tgcgttagtt ttcttgttct ccatgatatt
    15841 ggtcattgga tactacttta ttagtaaaga aaaccgtcca aaacggctga agaaaaacag
    15901 aagagtacgc gagggggtgg cacggctgcg atgaaaaaat tacaatctct gtttttaggt
    15961 attctcatca ttattgcggt gctgctgctt ggtgttcgtc aacttgaaaa aacaagtggc
    16021 atgagtggag caaaggtact taccatctat aactggggag attatatcga tcctagtttg
    16081 atcacgaaat ttgaaaaaga atatggatac aaagtgaatt atgaaacatt tgattctaat
    16141 gaagcgatgt tcacaaaaat ccagcaaggt gggacaaact acgacatcgc tataccaagt
    16201 gaatatatga tccaaaaaat gatcaaagaa aaactagtac ttccattaga tcattcgaag
    16261 atcaaaggat taaataatat tgatccacga tttttagact tggattttga tccagataat
    16321 cagtattcga ttccatattt ttggggaact ttagggatcg tatataacga taaattcttt
    16381 gatgcaaatc aaattaagca ttgggatgat ttgtggaagc cggaattaaa agacagtttg
    16441 atgctgattg acggtgcacg agaagttatg gggctttctt tgaatagttt aggctattct
    16501 ttgaatagta agaatatggc acagctgaca gaagcagcaa ataaattgaa tcatttaaca
    16561 ccaaatgtca aagcaatcgt ggcagatgag atcaaaatgt atatgatcaa tgaagaagcc
    16621 tccgtggcag tgacattttc tggcgaagct gccgatatga tgtgggaaaa tgaacattta
    16681 cattatgtga tcccaccaga aggatccaat ctttggtttg ataatatcgt tatgccaaaa
    16741 accgtgaaaa ataaagaagg tgcctatgat ttcatcaact ttatgctaga accggaaaat
    16801 gcggcacaaa atgcagagta tattggttat tctacaccaa atgaagaagc aaaagcgtta
    16861 ttgccaaaag acatttcgtc ggacgaacaa ttctatccat ctgatgacac gatctcccat
    16921 cttgaagtct acaaagatct aggatcgaaa tacctaggca tctataatga cctattctta
    16981 gaattcaaga tgtataggag ataatggcaa aactcagtaa gaatggggac ttatcaacgt
    17041 tctaaagagc taaaaatcgt cctttttcta tctgagtttt ccaatcttct accaaaagcg
    17101 gatgaaacgc cgcttttttt gtgttatttt attttttcta ccacgttttt ctaccagaga
    17161 aaagtggaca aaaccattgt aaaatagggc ccgagacggg ctagtaaatc taagattgaa
    17221 aaagttacag aaggataaag taattggaaa ctatgcaaat atacttaacc cttctgtaat
    17281 ctattaataa aactaatata tgaatccagt tcaatatttg tagctatgaa aaaacataaa
    17341 aatcatatta gataaaaact aaattggtag ataaattatt ttgtcagata tctgaggcaa
    17401 atattcgact atttgtgttt caaagtaaaa attggaatac aaatatagaa tagacatatt
    17461 gcagccgata gtttattatg cttttagaca cattaaactg gaggaaaaaa tgaaaaagag
    17521 cgctaaagat cgagtttttg atgagctgat acgactaaca gggaatatgt cttctgtcac
    17581 tgccgagaaa atttcgaaga atctaggtat ctctcgtcag aatgccagcc attatttgac
    17641 gagattagtg gaagataaaa aggtcgagaa attaccggga aagcctgttt tgtggaagcc
    17701 tttagatgaa tatgcggttg tagataatac ggagcaaata aacgaagcct ttcattctgt
    17761 agttggtcat gacggaagtc tgagagaggt cattcaaaaa tgtatagccg ctgtcaaata
    17821 tccgcctaac ggactcagtg tattaataaa tggagccacg ggtgttggaa aaagtttttt
    17881 agccacaaaa atttttgaat acgctatcca tgagcaaatt attgaaaaag atgcaccatt
    17941 tgctatctta aactgtgccg attatgcgga caatcccgaa ctgctttcgg caacactgtt
    18001 tggatacaag aagggggcct ttacgggggc tgaaaaggat accgatggtt tattggcaac
    18061 ggccaacaac ggctatcttt ttctcgatga ggtccatcgc ctatcgaaag aaaatcaaga
    18121 aaagctcttt ctatttattg atactggaaa ctatcgtcca gtcggtgaaa atgtaaattg
    18181 gcattctgct aaggttcgtt ttattttcgc cacgacggaa aaaggtgaaa attatttatt
    18241 agacaccttt gataggcgta ttcaaattag tgttatgttg ccaacttttg aagatcgccc
    18301 cattcgagaa cgattagagc tgattcaatt attttttcaa aatgaagcgg atgttttaca
    18361 aaaagatatt attgtttcaa gagaagcctt gtctttaatg ttatctcatc cctttagtgg
    18421 gaatattggt aagctgaaga atatcattaa aatcagttgt gcggatgtgt atagtcgaac
    18481 acaagaagaa ccattaacga ttgggaaaaa tgagatcttg attcagttaa atatgctgga
    18541 atccgaagta gaaagcttgc cgattgctag tttattgata aagaaaaatc aaaagataaa
    18601 aacggatcga atcaaaccgt ttgattatga ttcgattttg gaaaaaatta ctcagctgat
    18661 aaatgaaggt aatttcaatg aaaatttttc ttcgataaag aatgaagtac agcatttggt
    18721 gcatcacatt gataaggatt cttttttatc aatgagtaga aaattgatga ttcagatgtt
    18781 tagaaaggtc tgggtatccg ttttaggaaa aacgtacgga ttgacgacga gtggacctgt
    18841 tgcagaaatt attagcagat cctatgtcat ttccccaagc tataagacag acttaaccgg
    18901 gataaatcgt acattagctg caaatctacc tcggacgact tacttatgta atcagttctt
    18961 ggagcattta ccaccgctct cagaaaagga cgagaacttt ctaagactgc ttattgtcgt
    19021 gactttatct gattatgtag atgaatccat cgagctgaag ggcctgttgc ttgctcacgg
    19081 agatagtacg gctagcagca ttcaagcagt agtgaatcaa ttatgcggca attatgtttt
    19141 tgaagcgttg gatatgccag tagagagtaa tgtgtcagag attgtcgaat cgacaaggaa
    19201 tttcgtgaaa cggcaagtga atacagatag tctagtgctg atcgtagata tgggctcttt
    19261 aaatcaaatt tacacacaga tcaagaacga tttaaaaggg gagttacttc ttctgaataa
    19321 tttgacgact tccattgcac ttgatattgg cttgaagatg acgatgaatg cgccatttaa
    19381 acagattgcg gaggatgcag cagacaagta ttcaatcaat gttcaatact ttgaaggctt
    19441 ttctcaaagt agcaacatta tcatttcctg tatgtcgggt ttggggattt cggacaaatt
    19501 gaaagatgtt tttcgtcatc atttatcgga tgaatccatc gaagttttca cgaaggacta
    19561 cagagaacta cgtgaattaa tcgaccaaaa tgatgaaact tattttgaca agacaaaatt
    19621 ggtcatcacg acttcggatc ttccacgttc cttttcaatt cctaatatta atgtttatga
    19681 catcttagac ggtgatggag cagccaattt ggagcatatt ttgagtcctg aaatgaagcc
    19741 ggcagaattt aaccgcttga ttcaggagct agtaaaattc ttttcgttgg aaggaattgc
    19801 ggaccgtttg aactttctga acccaacgat cgtgatgagt gaagtggaaa cagtcatttc
    19861 taaatatgaa aattactatc atttcacttt aaatggccgt gtaaaactaa atctctatat
    19921 gcatacggca ttgatgatgg aacgtttatt cttggctcgg acagaaaaat ctgaagaacc
    19981 gaattatgat ttgaaaccag atgaagatga attttattat gtttctagaa gtatttttca
    20041 atcaatggag atgaaatata attttaaagt caatgcttat gagctttcgc tgctctatga
    20101 attattagga ccgtatattc aaaaataaag tgtctaaaaa agtgtctaat ctgttttttt
    20161 taggttagac acttttttta atgtgaatta acgtgattaa accaatgttt ttaaaagttg
    20221 gcacaggaat tgcttatata aaagtgaaca gaaagaaagg aattaaccat atgaagaaaa
    20281 ttctaatagc gactcacggt cacttagctt caggctttct tagcagtatt caattactga
    20341 cggggaaggt tcaagagatt acggtgatta atgcttatgt ggatgagtgt gattttgaac
    20401 aggagttgaa tgtatttgta aaccaggtga acgagcagga tcagattttt gtttttaccg
    20461 atttatttgg cggaagtgtc aatcagaaga ttaccaaaac ctttttggag aaagagattg
    20521 ctgtaactat tttgtctggc tttaatctgc caattctgct cgaaatagta ttggcaacca
    20581 aggaattaac aaaggaggat attcaggaac tggtaaataa atgccaacag gaattaaaga
    20641 tgaccattct tgatgaggta catgtagtaa atgaagagga agattttttt taggaggaag
    20701 aaaaatgatt aaagcagtgc gaattgatga acgattaatt catggacaag tagcgatgac
    20761 ttggacaaag agtctaaatt taaccgggtt agtagtagca agcgatgagg cggctaccaa
    20821 cgatattcaa aagatgacat tgaagatggc tgcaccatcg aatgtcaaag tcattattaa
    20881 aacagttgat gacgcgattc atttgttaaa agatccgcgg gcgaaggaca tgcgcttgat
    20941 ggttttagtc cctaatgtca aagatgcggt aaaagttgcg aaagctttcc caacggaaat
    21001 cgaattaatg aacgtgggga atgctgggaa aatgacaccg ccatcggaag ataagaaaac
    21061 cttatcaaag gaagtgatgt taacgacgtc agaaattcaa gacttaaaag aattagttga
    21121 actttatcca gataccgttt ttcagcctac accggcgatg gaaaaacgtt cagctatctc
    21181 tattttgagt gatttttaga aaaaggaagg agaaaaatta tgtatgatgc atttgtagtc
    21241 gctttggccg tttttattgg agttgctgga catgaaatgt tcgggatgtc catgctgagt
    21301 cgtccgattg ttgttgctcc gttagcagga gcgttgatgg gggatttgca gacggggttg
    21361 attgtcgggg cgtcactaga agccatcttt atgggtgtcg tcaatatcgg aatctctagt
    21421 acggcggagc cttctttggc tgctgggctg gctacagcgt tcgcaatcaa aatgggagga
    21481 aacgtggatg cgattattcc cattgtcttt ccactagcag tattaggatt acagttaatg
    21541 aatatgattt tttcttttgt atgtggaccg atggctgcta aatttttcaa atttgcaaaa
    21601 tctggtgatc aaaaaggaat catcaatctg cacttcttta tgtggtttgt ccactatgcg
    21661 ctttatgctc ttattccatt ctttgccgtt ttatttggag cagatgttgt caaaagcgtc
    21721 ttggataata ttccagaggt catcatgaac ggattaacag tcgctggaaa tctgctgccg
    21781 gcagttggga tggcgatgct gttacggatg ctttggagta aaaatattgc tgtgtggttt
    21841 ttctttgggg ccgttgttat ggcgtatctc gagttaccat tgattgcaat tgccgcagtt
    21901 ggaacgatta ttgcaattgt tatagcccaa agagatctac aactaaaaaa tatatctgtt
    21961 catgcagttc aaagcgatgc tgctgtaaat gcaacgcttg accaagaaga ggaggatttc
    22021 tttcaatgaa attagctgat ggaatttcta aagatgagaa aaaattggtt cggaagatgt
    22081 tttggcgctc gatgactatg tatgcttcgg tgaatccggt gacgatgggc ggcggtggtt
    22141 tcgcctattc tatgatgccc ttcattaatc atttctacaa aagtgaagaa gatcgagctg
    22201 cggcgctgga acgtcataca cagtattttt cgacgaccat tccttgcgcg tcctttgtca
    22261 tggggattgc ggcatcgatg gagaaggaaa attctgaaaa agctggtttt gacgtggaaa
    22321 gtatcaacgc gatcaaaacg agtttgatgg gaccattggc tggaattggc gattcattgt
    22381 tctggggagt ttggcgtgtc gttaccgcgg gactagcgat caacttagcc aatgccggaa
    22441 atattttggc accaattatc tttttagtta tgttcaatat acctaattac ttgatgcgat
    22501 actatggcgg cgttttaggc tattcattag gctctaaata tattgaaaag ctgtatgcga
    22561 acgggttaat tgaaattctg acaaaagctg ctagtatctt gggtctgatc atggtaggtg
    22621 caatgacgat cagtatggtc aaattcaata ccgttttgag cttttcaatg aagggcgaag
    22681 aactaatgaa tgtccaagaa atcctcgata gtatttttaa aggaattgta ccgttattgg
    22741 taacattgag ctgtttcaaa ttactagata aaaaagtcgg cgtcatcaaa attatttttg
    22801 gaatggtcgt cttgggaatt ttattgtcat tacttggaat cgcatagtag gaagctgcag
    22861 ctactaggct gcagtttttt tagcaaggag ggaaaagatg actattaatg aagtaattta
    22921 ttcacccaaa cacccatttt ttaatgctga gttaaacgac ggaaatgtga ctacttcaaa
    22981 cgaatacagc tactatcgga atattcctga tgtaaaggga ttgaccttcg atgtaagtaa
    23041 tcaaaagaca ttagcaaacc ttgatgtttg cgggacgatt caaacgttga ccttttatcg
    23101 agaaaattta ctaactgagg aaaaaccagg tgtttgggtc aataaacagc tctctcaaac
    23161 caattcgtta tccttaaagg ttgaagtaaa cgggattgtc taccatttgt cagaatcgga
    23221 tcatcgtatt gaagtcgatg tgattcaaga ctgtttgcct agatatattc atcattattc
    23281 gacgtttaag gtcattgtag tatcctttgc tcccatactg gctaataagc gtttaagtat
    23341 gcttgttcaa caggtttaca ttgtgaatga aacacacgaa agccttcaag tttcagtgaa
    23401 agatgtgcct tcgtatcaag agaagtattc ggatcagcaa aatgtgttga tttctcaaaa
    23461 aggaaataag gacacgatcg cacaacagga agtggcaagc ttcgccattg cttttgtgga
    23521 cccaaatgct tttgaagaag taatgacttt tcaggaatct gatatcgaaa aatggctgaa
    23581 ggaaacattg aactattttg ctcagctatt tgggcagctg tcaatgccaa acgatagagt
    23641 tgtccatctc tatcgtcgtg cactttatca aagcttttct agtttcggta tgaatcgaca
    23701 gggacaggtc gtcggcagca attggggcag ctatcctgct actaatcgaa tttggaataa
    23761 ggatatgtat tacgcatcat tgccattcac catgtttgaa ccggagttat gtcaaaaaac
    23821 ggttctatgg tttgatcaat atggcgttaa gtttcctggt tcaaaattta caggaggtat
    23881 caatcattct ctgagtaatt cgttatccag tgcgttactg gctagtcttt attttgaaca
    23941 tactggggat acaaaattct ttgagcggtg tccgcaggtt ctactgaatg cttcaagaat
    24001 tattgaggac atattagctc aacgtcggtc aggggaacca atgctttttc catctgtttg
    24061 gttatcagac gcctttgcac ttggaaagta tcataccggc agcaatcttt gtctttggaa
    24121 ggcctgtatt ggacttgcag aaatttttga agccctagaa caactgagtc ttagcaagcg
    24181 atacaaaaac attgcctgta aactaaagga atcaatttgt aaacaaatga ctatcgatgg
    24241 tccttttggt gagcaatttt tggagggaat cggtgatgag gagaaaacaa cctattcagt
    24301 gaagcactat caaaaaccga ttcttgagca agggttaatc tttttaagtg atgtgattgt
    24361 ggatggaaaa attaatttat tgatgcatga tggagaagaa tcagacacta cgttgatgcc
    24421 tttttatcaa tttttagata ataaagatca tttatatcag aacaccatgg ccttttctgc
    24481 tagttctttc aatccaacct atagcgagga aatcaaaggc atcacttggg gattagaatc
    24541 cggcgccact tttcctggct ttatcactgt tttaatgagc gagctgcaca accctcaagc
    24601 tttcgtaacc cgattagaag aactaatttg tttggcagat ttagacggtt cgtggtggtg
    24661 gtggccgtat cgcttagagg ctcaacaagg agaagtcgtg cgagactttg gctgcggcaa
    24721 atgcggctgg gcatcgggtc tttttgtcag cctaatgatc agtcaatatt tcggattgaa
    24781 attcaaaaaa ggagtattgc aaattgatcc tgttgataat ttaacgtttt cttggaagaa
    24841 tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg caatccgagt tgagtatttc
    24901 aaatttgtca gaaaagccat tgaaaataag tgatacgaaa aaaattcttc acgtagaaaa
    24961 gggaaaaacg attcacattc aaaggagaaa aagatgagta tactaaaaaa tattacccaa
    25021 tttaaaaaaa tccagagtcg agcaatcact cccgaaaatc caacaggcga gaaaggtaaa
    25081 gctgcgatgt tgccaagcga gctaggacca agccgcaaag gtcgtggcgc aattccgctg
    25141 acttctggtg aagaaactgt gattgcggat attactggaa cgggaataat tcgtcatatg
    25201 tggatgacga ttcgtgatcg aacagaattt ggcagttttg ttttacgtga tttaatctta
    25261 agaatttatt gggatgggtc ggatagccct gctgttgaaa gtcctttagg ggatttcttt
    25321 tgtaatgggt ttggagagcg ttgcgaggtg aactcgttgc cgatagtggt taatcctaca
    25381 gggggcttta attcctattt cgaaatgcct ttccaaacat ccgctaaaat tacgatcgag
    25441 aatttacatc cgaaggatat gtcctcattt ttttatacga taaattacgc tttggtggac
    25501 gaattggaag aaaatagcct atattttcac gcaaattggc atcgggaacg cattaccaaa
    25561 ttacaggagg attataagtt aattgatcga ttaaagggtc aaggctatta cgtgggtact
    25621 taccttgcgc ttacctgcct agaacgttat tggtggggcg aaggcgagtt taaattctac
    25681 attgatgggg atagtgagta tccgaccgta acctcaacag gttcagaaga ttattttggc
    25741 ggggcgtggg cctttcatga gtacgatgag ttcggacgtc cacatgcgaa aaactattca
    25801 acagcctttt tagggtatcc ttaccaatca aatagagatg ccacaagaga tcgttttgaa
    25861 acgggaaaat tgaatgccgt tcatgccttt ggtgacgacg caatgccacg acatggtctt
    25921 tatcgttggc atcttcaaga ccccatctgt tttaccgaag atttatcggt cactttacaa
    25981 caaatcggca acgatgatgt tcgcttattt gagcggcaag acgatgttgc tagtgtcgct
    26041 tactggtatt caacagaagc ttcaggaaat actaaacgtc atttcactga tcgagaacgc
    26101 ttgccgcgtt aatgaagtgt ttttcacata ttcgaaggga ggaacctatg atgatatatg
    26161 gggcaattga agctggaggg actaaatttg tctgtgcagt cggtactgag gaattaacga
    26221 ttttggaaag agtcagtttt cccacaacaa ctccagttga aacaatgaag caggtcattg
    26281 atttttttaa accttttaag gaacaattgg caggcatcgg ggtcggctgc tttggtccaa
    26341 ttgatattca atgcaaatca acgagtttcg gtcatatcac atcaacacca aaacttgctt
    26401 ggcaaaattt caattttgta ggaacgctaa agcaatattt caatattccg attgaatgga
    26461 caacagatgt caatgcggct tgctatggtg aatatgttgc tggttctggc aaagggttag
    26521 caggtgttgc atacttcact gtgggaacag gtatcggagg tggtgccttg ttcaatggga
    26581 ctttcgtgga agggttcagt catccggaaa tggggcatat gttgatcaag cgacatccaa
    26641 aggacaattt ttcaggaaac tgtcccttcc accaagattg tttagagggg atggcagcag
    26701 gaccagcgat tgaaaaacgg ttgggtgtaa aaggccagaa tttattggct gacgattcgt
    26761 tttggcagat tgaagccttt taccttgccc aatgtgcata caatacaaca ctgatgtttt
    26821 cgccagatag aattattttt ggcggtggtg taatgaaaca agaacacatg aagaaaaaag
    26881 ttcaggataa atttgttgag cttataaatg gatatgttga aataccacca attgatagct
    26941 acattattac gcccgaatta ggtgataatg ccggaataat cggcggatta gcattggcca
    27001 gaaaagcggt aagaaataag caaccataat taatgaatgt ttcattgata ctcatgtcaa
    27061 aagtatttaa ccgagtaatc aaatgatcaa atgaagttgg ataaagcgaa aatgaattca
    27121 aataaaaagg ctctaagtca aatcgtgttg ttttcattaa atcactgaaa ggaaatgctt
    27181 ctgcgtttcc ttttttcttt ttgttgtgcg ctgtatacct tgaggatatt cactggtggc
    27241 gtacacttaa atagtaatat attgtgttac tttatgtctg tggtaatagt tatagttgct
    27301 taccaatgta tgttcatttt tcgaaatgtt gacttcataa tccttggctt aatgtttgaa
    27361 tttttttggt gatagatttt ttcagccgta taaaataaat cgtaattcta ttatagagag
    27421 ttgtgattat aaaaagatag caagaaaaaa aaagaaagtt tctatacatt ttaaatattt
    27481 tattcctatt tctattgcta aaaaagtaga aattctat
//
LOCUS       DAHHPA010000029        27486 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.030, whole genome shotgun sequence.
ACCESSION   DAHHPA010000029 DAHHPA010000000
VERSION     DAHHPA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.030"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(176..1462)
                     /locus_tag="NBH55_001151"
     CDS             complement(176..1462)
                     /locus_tag="NBH55_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD1509148.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="NBH55_001152"
     CDS             complement(1455..2723)
                     /locus_tag="NBH55_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD1509149.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="NBH55_001153"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="NBH55_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCD1509150.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="NBH55_001154"
     CDS             complement(3495..3941)
                     /locus_tag="NBH55_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1509151.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="NBH55_001155"
     CDS             complement(4034..4279)
                     /locus_tag="NBH55_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCD1509152.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="NBH55_001156"
     CDS             complement(4394..4972)
                     /locus_tag="NBH55_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD1509153.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="NBH55_001157"
     CDS             complement(5049..5579)
                     /locus_tag="NBH55_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCD1509154.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="NBH55_001158"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="NBH55_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCD1509155.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="NBH55_001159"
     CDS             complement(6436..6831)
                     /locus_tag="NBH55_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCD1509156.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /locus_tag="NBH55_001160"
     CDS             complement(6828..8240)
                     /locus_tag="NBH55_001160"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCD1509157.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="NBH55_001161"
     CDS             complement(8237..8773)
                     /gene="cysE"
                     /locus_tag="NBH55_001161"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCD1509158.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8859..9098)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9095..10555)
                     /locus_tag="NBH55_001162"
     CDS             complement(9095..10555)
                     /locus_tag="NBH55_001162"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCD1509159.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="NBH55_001163"
     CDS             complement(10725..11882)
                     /locus_tag="NBH55_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCD1509160.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="NBH55_001164"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="NBH55_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCD1509161.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="NBH55_001165"
     CDS             complement(13349..13876)
                     /locus_tag="NBH55_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCD1509162.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="NBH55_001166"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="NBH55_001166"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCD1509163.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="NBH55_001167"
     CDS             14909..16156
                     /locus_tag="NBH55_001167"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCD1509164.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="NBH55_001168"
     CDS             16232..16576
                     /locus_tag="NBH55_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509165.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="NBH55_001169"
     CDS             complement(16693..17019)
                     /locus_tag="NBH55_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509166.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17634..18836)
                     /locus_tag="NBH55_001170"
     CDS             complement(17634..18836)
                     /locus_tag="NBH55_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1509167.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            18953..19468
                     /locus_tag="NBH55_001171"
     CDS             18953..19468
                     /locus_tag="NBH55_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCD1509168.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19667..20113)
                     /locus_tag="NBH55_001172"
     CDS             complement(19667..20113)
                     /locus_tag="NBH55_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCD1509169.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="NBH55_001173"
     CDS             complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="NBH55_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCD1509170.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21992..22621)
                     /locus_tag="NBH55_001174"
     CDS             complement(21992..22621)
                     /locus_tag="NBH55_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCD1509171.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(22605..23069)
                     /locus_tag="NBH55_001175"
     CDS             complement(22605..23069)
                     /locus_tag="NBH55_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1509172.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23556..24824)
                     /locus_tag="NBH55_001176"
     CDS             complement(23556..24824)
                     /locus_tag="NBH55_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCD1509173.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(25055..25792)
                     /locus_tag="NBH55_001177"
     CDS             complement(25055..25792)
                     /locus_tag="NBH55_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1509174.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25859..26428)
                     /locus_tag="NBH55_001178"
     CDS             complement(25859..26428)
                     /locus_tag="NBH55_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCD1509175.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26556..27428
                     /locus_tag="NBH55_001179"
     CDS             26556..27428
                     /locus_tag="NBH55_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1509176.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         7743 a         5827 c         4955 g         8961 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataac gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat
    17281 cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt
    17341 tgtttctcta atacttagga taaaacgctg attttacaac ctctgttaaa cggtgttcaa
    17401 tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt
    17461 tgtttctcta atactcagga caaaacgctg attttacaac ctcctgataa acggtgttca
    17521 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt
    17581 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctgat ttcttattct
    17641 tcttcatttg tagcaaaata attacggtga taaatgatac aacatacact gataagaaca
    17701 atggcacctg caaaataaaa cgcatagctt tcaccaatat gttcaccaat cacacctgca
    17761 ataaatggtg ctaaagctcc accgaaaaag cggacaaaac tatagctgga cgaagcaacg
    17821 tttcgttgca gacctggtat ttccatagca atcgtcgtca acagtgtatt gataattcct
    17881 tggaaaaatc ctgctaacac aatgctgatc ggtacaagga ctggcataga agttccagct
    17941 cctaataaca gcaaacaaat aatgaataag acaaatgctg ctaaaattgt ccgataagtc
    18001 gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag caatagccag aagcacaccc
    18061 cagccaaaaa atacaaaacc gacttctaat tcagaatagc caatcaataa aaatggcgca
    18121 tacgcaagta aagtaaagaa tccgaaatta tataacaatg cgattagtcc aactgaacga
    18181 agtttatgat tgcttaacgc tttgacacct tcccaaaggc ctacttttct ctttggtttc
    18241 tcagtttcag gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa aaatgctacc
    18301 ccaaaaaatg gataacgcca tgaaccactg cccaagaccc caccgactag agggccaaca
    18361 gccattccgc agccaagtgc tgcttcatac atcatgattg ctttttctgt gttaccaact
    18421 aaaatactga cgatcgttgc taaagcagta gagacaaata aagcattccc gacaccccag
    18481 ccggcacgta acccaatcag cattccgata ttcccagata atccgccaag accagcgaat
    18541 acaacgatga taaacaatcc tgtcatcaat gtttttttca aaccgatccg actagataag
    18601 aaacctgtaa atagcatcac cacaccagtg actaacatgt aactagtaaa taacagggtc
    18661 gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg gtctactaga
    18721 cccactccca taaaagcgat catacaggta gctgctacaa ctagcgctga acttttcccc
    18781 tttgtttctt catttgtttg ttcttgtata tctatttgat tctccaatat ttccatccct
    18841 ttctctctat tagcatcata catgatttat tttacatgtc aatatgcaca tgtaaaacgt
    18901 ggaaacttta ctgtgatata ataaagaaaa taagacgaga ggtaggattt ttatgaatac
    18961 tttagcctat atattattat gcgtgctttt atccaaacca cgttctgggt atgaattgaa
    19021 gcagctgatc acaatttttt gggaagccca tcacagtcag atctatacaa cgcttgccaa
    19081 gttagaccag cagggttatg tgaccgtttt ggacaaagac gagcactctc aaaaaaagat
    19141 ctaccacttg actgatgaag gacgtgctct tgtagaggaa tggatcaaag aggaaactcc
    19201 cgctcctacg caaaaagatg aatttctagc aaaaatctat gctttttcga cgctcgataa
    19261 aacaacagct actggactgc tattcactag agaacaacag ctgaacaaaa tcatgcaaaa
    19321 aaatcaaaaa aaattgaatg acctctcgct agacaagaaa caagaatttg gacgttatgt
    19381 tgtgatccag cgcagaatta tgctttgtca gcaggaactc caatggtgtc agcaagtaag
    19441 agaacaaatg cagcagtttt ttgagtaagg gataaacaga acctttctag aaatatctat
    19501 tgaagagcaa attatcttca ataaaagagc aatacagttg gaaaagaaaa agcgatccga
    19561 acgacaggcc aaaacctatc gttcggatca ctatttttct agaactgcat tcaagagact
    19621 atgacaaaaa gcatgccaag gccttttttc aaataacagt gttccatcat tcattacttt
    19681 gaattgcttt tagcacactt cttgaaagcg ggccaacgat gatcaactgc aacggtaaag
    19741 cggcaatcac attcattttc caagcattca tataagcaga gaataattga tcaggtattc
    19801 cttgttccat gatcacaccg aataaagaca tacatgtcac cattcctaaa accatcaagc
    19861 atgaaatagt taagatcatt tggaccttgc tttctggatt tactggtagt ttaaaggcaa
    19921 tttttttcgc cagcacaccg atgatgaaga catccaaaat gaaagccacc acgaatccag
    19981 gcactagacc aatcaacaaa cttgataaat gaagatcttg atgcagccat aaattatacg
    20041 cgctcatccc caagaccatc aaaaaacaca tcattgttgt aaaaattatt ccttcttttt
    20101 tatttgtagg caatctgtac cactccttta aatttgtcac gaaaatcatc gtagcacatt
    20161 taaaaatttc tcgtaagtac tttttttatt ttctgccctt ttatttcata aacttcatca
    20221 gccgtacgct cgacaaagta agaatcatga gaagtaaaca aaatcgttcc cggatatatt
    20281 tgcattagct tctctaaagc ttcgatcgtt gccatatcaa taaaattagt cggttcatct
    20341 aacagcaaca cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct
    20401 cccccactta atgtacataa cggtttcgtc aaagtttcct gagcaaaccc taagtgattc
    20461 agcaaactac ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg
    20521 gattcttcag atgtcatttg ataacccttt tgctgatata cttgaaagct tactttcgga
    20581 gagagaagga ttccttctcc ttgtttgcgg atatgttcta ataaggttgt cttacctgaa
    20641 ccattttcac cgacaatcgc gattttttta cctaaagaaa actgaaaatc accatttacc
    20701 agtaatgttc tcccacttct ttccaattgg acattttgtc ccatgatggg atagcggctg
    20761 tggagttcaa gagattttgg tactggaaaa cggatttgtt tcatctgctg cggcttggtt
    20821 gtttctccta ttttttggag tcttctctct aatgttttcg cattcttttg gatggctttt
    20881 tgtacactgt ctttttgttt agaggaagac aaccgatcca gtctgatttg ttgctttttc
    20941 tttttactgg acactttttc caacttttcc gcttgttttt tcttttcttg gatcgatttt
    21001 tccaatcgtt ttttctcttt catgatcgac tctgcttctc gtagctgagt ctttttctcc
    21061 aattcctttt gagtgagata ggcactataa ttccccgaaa actctcggat ttctccgtct
    21121 tttacttccc agattttcga tgctagctca tctagaaaat agcgatcatg actaacaaaa
    21181 accagcgtac cataataata acgaagctct tcaatcagtc gatcaatact ttttctatct
    21241 agatgggttg ttggctcgtc tagaagcatc ccttgttcat aagaagaaag aagacgtatc
    21301 aactcgatct ttcgttcttc ccctccgctc catttccgct ctgaagtttc ttccagtctt
    21361 aactggaaat gactggctaa ttccaaattt aggtcctttg cttcagcgag ataggttaat
    21421 tgaggcaggt agttgaaggt gatctctgtt tggattttcc catgatcggg aaaaagttca
    21481 ccggctatta gtttgagcaa agtcgattta ccgacaccat tttctcctat gatccctata
    21541 cgatcttgct gatagacacg aagagaatca atagatagtt ctagctggtt gccaaagctg
    21601 acttgtagat ttgttatatt tactgctaaa ttttccatat gttttacact cctatttcat
    21661 gtaaaacaac aattatcgat actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc
    21721 atcacatctt ttcctactct gaacgaaata actatgacta aatcaccaaa tctgtcatag
    21781 ctttcaccta ttctactgtc gttcgaaaaa atggacatac tccatccatg aaattgttgt
    21841 ttatacatgt cttttgaatt tatttagttg ttattcaaaa gttcgaaacg taatttcatc
    21901 gatgcagtca tttttccgac ctcctttttt attaggttac cacacacgat tcaaaaaaag
    21961 caatagctac tgaatagtaa ctgaaaacct tttattcgaa catagtgatc ttttctggtg
    22021 ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc tattgataaa ataataactg
    22081 gaacataacg gtctttatcc aaaccaaatg cctctgccaa ctgatctgct tcgaatccac
    22141 caattggatt tgtttcatag ccatgatcac gcgcaactaa catgaattgc atagccgcta
    22201 agctagaatc gactttgact acatcgttca tttcttctct tgaaaaactc ttgtaataag
    22261 gaatgatcgc agctaattgt tgatctctga cttcttttgg catcttccct gattcgtaag
    22321 cttggttgta gatttcttcg ccatattcgt aacattgcat atcgccaaaa atcatgacca
    22381 ttgcagaaga agtatcattt tgtctcgtat tgaaacggat caaaggacgc aagatttctt
    22441 ttgcttcctc gctttcagcg accacaaagc gccatggttt catattgaca gaagaaggtg
    22501 cagtcgtcgc ttctttgatc atttccagca tttcttcgtg tgggattttg tatgtttcat
    22561 catatacgcg aacggatctt ctaccagtca taattgttga aaaatcattt ttttgttcca
    22621 ttattattaa cctctttcgt ttctgttgtt atattgcaaa gttgggtatt caatttatct
    22681 aaaagtgtta gaaattgttt catttctaca tcgctgaaag ccggggatag cacggaacct
    22741 gtatctttat ggtccatttc acaatgcttg attttcttct gggctttttc tgttagcgct
    22801 acatacactt cccggttgtt ctgaggattt ctctgacgcg aaacatagcc atttgcttcc
    22861 aataccttca gatgacgagt aactgctcct tgatcaatct gcaacttttc ttgcaaactg
    22921 ctttgtagca atggattttc ttctttcagt ctcatcatga tctcgtatct agtaagactt
    22981 ataccgatat tttcttcaaa gatctggacg attttttgat cggctaattt gatttgatat
    23041 aaaaggtgac taatctcttc taaagacata atttcctcct ttctaataat tgactcgtca
    23101 acgattgact tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa
    23161 taaaaaaaac gctctcaata gaaaagtata gagaaaagaa attttttgta gaatgaacgg
    23221 aaaaaatgat aaagaaagca caacgctaaa accaagatta ttgattctta tcaaactata
    23281 gaagatagct cttctgcagt agataatttt acaccgccaa aaatttccga aatcgccttt
    23341 tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg cttaaacagt aataaaaaag
    23401 ttatgacaag atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg
    23461 cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac
    23521 tacttgatgc agggcagctt tttcactacc tctttttaat aaccataaaa tcctattcct
    23581 tcatatctcc agcccatcgg tgttagccaa tctctttcct tagcactcaa ggtatagtga
    23641 tgggtaccag ttttttcatt tggattaaat agtcgataaa ttggcttgga accaccagaa
    23701 taaaaactta gaccttcata acgccaaccg atttttgtta aagagttgat ctcagattgt
    23761 gccattgtgt aatgatggtc tccactgtaa ggattgtata aacggtagac aggttgtccg
    23821 ctgtttggtg cttgccaagc aatcccttca tattcccagc ctacagttgg cagataattt
    23881 ttttctccgg cattcaatgt atataaatgt tctcctgtat gacggttata cagacgatac
    23941 atcggagaag ttgaagttgg tgctgtatca tactgtgtca gattataggt ttcgattaca
    24001 ttgttcaatg tagaaccata attaggcgca gtggcataac gacctgttag ccaagccgta
    24061 gcatctttgt aagagttcgt attgcttttc catgcaccgg aatagtagta aacgcctggc
    24121 tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca atgattcttt gtacgaagga
    24181 tattttctaa aagcggcatt caccgtaatc cattgcccat tcacatattc cgaagtaggc
    24241 atcgtaacag attggccatt atagcttcct ttaatcccaa atagattgta attaggggca
    24301 gaagccaaag tactgttccc ccagccactt tcaacgatgg cttgagcaat catgactgat
    24361 gcatataagt cattggcact tgccaaactt tgtgcagttg aggcaatact atcaatgaac
    24421 gcttgttgag aattagcaga tgagcgagca taaaaagcat agccagtata gttttcgaat
    24481 tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg
    24541 atttcttctg cattctctgg attgttctga gcttctgctg atgtactgtc ttcggtttgt
    24601 gtttgttctg ttgtttcagt tgttggttct tcggttgaaa ttgtattttc tgagacagta
    24661 gctgactgca aagtactttc tgtatcgttt tcaattcctg tttctgaaga actcgatgtc
    24721 tgagttgcga cagtttctgt cgattcttca gctagcactg ttgtgttggc aactgctaga
    24781 tttcctaaaa gaataacgct aaataataaa ttctttgctt tcatcttttt tctcctttga
    24841 attatgaaac ttttcctaaa taacagtgta actaaaaaag taaaagcaag tcaatgtcca
    24901 tatttaaata acatttgttt ctttttaata aatttgcttt ttctgcataa aaagacgtaa
    24961 atgaagaata ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat aactatttaa
    25021 caagtaattc tctctggata ttatccgtta tggattatac ttcctgtgtc gtcggacgga
    25081 taatcatgtt gctgacagac atccggtctg gcgtttctat cgcaaatgct acagcagaag
    25141 ctatatcttc agatgttaat ccccattctt tttgcgcttc atgaagttca agagcagcct
    25201 ttttgtcgct gatggtttga tataattcag tttgaacagc tcctggagag atgatcgtag
    25261 atttgatgtt attttgccgt tgctcttgac gtaatccttc catgattgca cggacagcaa
    25321 attttgttcc gcaataaacg gcggaatcag gataaacaac atgtcccgcc acagaatcag
    25381 ttgaaatgat atgcccagat ttttgttttt ccatgattgg aagtacggcg gaaattccat
    25441 ttaacacacc catgatgttg atatcaagca tattttgcca ttcatcacgg tgaccttcaa
    25501 ctaatggagc ggttggcatg attcctgcat tattgtacaa tacatcgatt cgaccgaact
    25561 tttccatcgc taaatcgata actgcttgga cttgtgcaaa atctcttaca tctgctgttt
    25621 gaatatatag ctcagcttct ggtaaagatt cttttattgc ttttaggcga tcttctcggc
    25681 gtgcagctat aactagttta gccccttttt ctgctaatag cttcgttgtt gcttcaccga
    25741 ttccgcttga agcgcccatg atgacaatca ctttttctga taaatcattc attctattct
    25801 cctcctgttt tgaaaaatct cttgcttata ctcactatct cttatttcat cttactgttc
    25861 aaataatccg ttcgacaggt ttgacgattt tggcagggat tcctgcaacg atggtattgt
    25921 ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta
    25981 cgcctggcag aatagttgct cctgctccga tccaagcatt tttcttgatg tggattggct
    26041 ttactgacaa gcctcttcgt tcttttggat cggttagatg gttcaccgtg atgaggttga
    26101 cacgaggacc aatcaatact gaatcttcta aacagatgcc acctaagtca acgaacatca
    26161 cttgacgatt gataaagaca tttttcccta tactaatatg tttgccgaaa tctgtataaa
    26221 aaggcagcag aacagtcacc gtatcatcta ctttgtcact aatgatattt tctagccgtt
    26281 tgcggatatc tgctggttca tggtacccag tattcagcgc tgctaaaggc tcttcgtttt
    26341 ccgcttggat ctgatggatc acttcaaaga gcggatccgt tactcgaatc tctttattga
    26401 cgatttgttt ttcgatgagg cttgtcattt tgctccctcc ttttatcctt actttataac
    26461 ggacggctta tgatgtctaa tacttataaa acatacttaa ctattccttt taactatact
    26521 tagtggtatg cttttttaaa aacaggaggg aaaaaatgga tattcgtata ctgacgtatt
    26581 ttttagcagt agctagagaa aaaaatatca gccgagcagc aaaatctctg catatcacgc
    26641 aacctactct ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg acacttttca
    26701 ctcgaggctc ccgagagata cagttgacgg attctggtcg atatctttat tcaaaaggaa
    26761 aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct
    26821 ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc
    26881 ttcattcgtt gatcaaggcg catcctgata tccagtttca tttatatagc gggaatgcag
    26941 atgatatcat agacaaactg gatagcggat tacttgattt tggactcgta atcgaaccga
    27001 caaacaaaca aaaataccag tcatttcgac tgccagccca agatcgttgg ggacttttga
    27061 tacatcggaa tcatccttta gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg
    27121 ttcctttgat catttcccgg caaacgtcag tggacagtca actagctgaa tggctaggtt
    27181 tcagcataga tcagttatcg atcgtcggaa catataactt attgtacaat gcttctttga
    27241 tggtcgataa cgggactttt ggtgcattag ctctagatgg gatcatcaat acttatggga
    27301 cggaaaaagt attcatccca ctcgatccgc cattacaagc ttcactgaat ctcatttgga
    27361 aaaaagacca gcctctttct cctgctgctc gtaaattttt agatgtccta acggagaaat
    27421 acagttaagt agagtttttt tccttcgctt tcagcttttt aacaacgaat aagttaagca
    27481 aaagat
//
LOCUS       DAHHPA010000030        26961 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.031, whole genome shotgun sequence.
ACCESSION   DAHHPA010000030 DAHHPA010000000
VERSION     DAHHPA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.031"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            4..861
                     /locus_tag="NBH55_001180"
     CDS             4..861
                     /locus_tag="NBH55_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCD1509177.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            976..1896
                     /gene="pstC"
                     /locus_tag="NBH55_001181"
     CDS             976..1896
                     /gene="pstC"
                     /locus_tag="NBH55_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCD1509178.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1896..2780
                     /gene="pstA"
                     /locus_tag="NBH55_001182"
     CDS             1896..2780
                     /gene="pstA"
                     /locus_tag="NBH55_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCD1509179.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2795..3601
                     /gene="pstB"
                     /locus_tag="NBH55_001183"
     CDS             2795..3601
                     /gene="pstB"
                     /locus_tag="NBH55_001183"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCD1509180.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3617..4375
                     /locus_tag="NBH55_001184"
     CDS             3617..4375
                     /locus_tag="NBH55_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCD1509181.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4388..5065
                     /gene="phoU"
                     /locus_tag="NBH55_001185"
     CDS             4388..5065
                     /gene="phoU"
                     /locus_tag="NBH55_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCD1509182.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5316..6334
                     /locus_tag="NBH55_001186"
                     /pseudo
     CDS             5316..6334
                     /locus_tag="NBH55_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
     gene            6331..7098
                     /locus_tag="NBH55_001187"
     CDS             6331..7098
                     /locus_tag="NBH55_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCD1509183.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7198..8766
                     /gene="liaX"
                     /locus_tag="NBH55_001188"
     CDS             7198..8766
                     /gene="liaX"
                     /locus_tag="NBH55_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCD1509184.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9063..9383
                     /locus_tag="NBH55_001189"
     CDS             9063..9383
                     /locus_tag="NBH55_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCD1509185.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9383..9733
                     /locus_tag="NBH55_001190"
     CDS             9383..9733
                     /locus_tag="NBH55_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCD1509186.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9900..10838
                     /locus_tag="NBH55_001191"
     CDS             9900..10838
                     /locus_tag="NBH55_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCD1509187.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10853..11683
                     /locus_tag="NBH55_001192"
     CDS             10853..11683
                     /locus_tag="NBH55_001192"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCD1509188.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11727..12752
                     /locus_tag="NBH55_001193"
     CDS             11727..12752
                     /locus_tag="NBH55_001193"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCD1509189.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12767..13705
                     /gene="galU"
                     /locus_tag="NBH55_001194"
     CDS             12767..13705
                     /gene="galU"
                     /locus_tag="NBH55_001194"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCD1509190.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13830..14270
                     /locus_tag="NBH55_001195"
     CDS             13830..14270
                     /locus_tag="NBH55_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCD1509191.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKATAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14273..14875
                     /locus_tag="NBH55_001196"
     CDS             14273..14875
                     /locus_tag="NBH55_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1509192.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14934..16040)
                     /locus_tag="NBH55_001197"
     CDS             complement(14934..16040)
                     /locus_tag="NBH55_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD1509193.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16250..17251
                     /gene="ccpA"
                     /locus_tag="NBH55_001198"
     CDS             16250..17251
                     /gene="ccpA"
                     /locus_tag="NBH55_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCD1509194.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17588..19933)
                     /locus_tag="NBH55_001199"
     CDS             complement(17588..19933)
                     /locus_tag="NBH55_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD1509195.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20212..21114
                     /locus_tag="NBH55_001200"
     CDS             20212..21114
                     /locus_tag="NBH55_001200"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCD1509196.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21327..23102
                     /locus_tag="NBH55_001201"
     CDS             21327..23102
                     /locus_tag="NBH55_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCD1509197.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23168..24754)
                     /locus_tag="NBH55_001202"
     CDS             complement(23168..24754)
                     /locus_tag="NBH55_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCD1509198.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            25005..25229
                     /locus_tag="NBH55_001203"
     CDS             25005..25229
                     /locus_tag="NBH55_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCD1509199.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25371..>26961
                     /locus_tag="NBH55_001204"
     CDS             25371..>26961
                     /locus_tag="NBH55_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1509200.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAH"
BASE COUNT         8617 a         4810 c         5651 g         7883 t
ORIGIN      
        1 ctaatgaaaa aacttaaatt attcttacca ataataggat tagccattct tttatcaggc
       61 tgttcaaagt ggattgatcg aggagaatcc attacggctg ttggctcttc tgctttacag
      121 cctttagtag aaacagttgc tgaaacttac cagacagaaa atcctggaaa attcatcaat
      181 gtacaagggg gcggaagcgg aactggtttg tctcaagtac agtcaggagc agtcgacatc
      241 ggaaactctg atttgtttgc ggaagaaaaa tcggggatcg atgcctcagc attagtcgat
      301 catcgagtag cagttgttgg gatcactcct attgtgaaca aggatattgg cgtaaaagac
      361 atcagtatgg aaaacctgaa aaaactattt acaggcgaga tcaaaaactg gagtgaagtc
      421 ggtggaaaag atcagccagt agttatattg aaccgcgctt caggaagcgg aacccgtagc
      481 acgtttgaaa aatgggtatt ggacggagaa acagctgttc aggcacaaga acaagattca
      541 agcggaatgg tgcgccagat cgttgccgac acacccggtg caatcagcta tgttgctttt
      601 tcttatgttt ccgatgatgt agatacgttg aatgttaacg gtgtagaacc gacagatgaa
      661 aatgtgacaa cgaacaaatg gacgatttgg gcatatgagc atatgtacac aagagatcat
      721 ccaaaaaaac tgacaaaaga atttttagac tatatcttat ctgatgaagt acaagataac
      781 atcgttggag aactaggtta tatccctgtt tctcatatga aagtcgaacg tgattggcaa
      841 ggaaatatcg ttaaagagta atctttacac aaaatttaca aacttagcta aacttttaca
      901 tgttattaaa accaaattga tgttcaattt gtacactgag tgagtatgtt aaacctgatt
      961 aagggggagt catctgtgga agatgtgaaa aaagtattat taacaaaatc aaaacgttca
     1021 aaaatggaac agcgtggaaa attcatcagt tttttatgta tcgcgctgat cgtgttggtt
     1081 gtattatcta ttttttattt tgtagctagt aaaggattgg ccaccttttt tactgataag
     1141 atcaatgtat ttgattttct gtttggtaca gattggaatc caagccaaat cggagcaaat
     1201 gggaaaccaa tggtaggggc attgcctatg atcacaggtt cgtttattgt gaccttttta
     1261 tctgctatcg ttgccacgcc atttgccatt ggtgcagcag tattcatgac ggagatctct
     1321 cctaaaaacg gaacgaagat cttacagccg gtcatcgagc tgcttgtagg tattccttct
     1381 gtcgtttatg gttttatcgg tttatcagtt atcgtgcctt ttgtcagaag tatttttggc
     1441 gggacagggt ttggtatctt agctggtaca tttgtgttgt tcgttatgat cttgccgact
     1501 gtcacaacga tgactgttga tgcattaaag gcagtgccac ggcactatcg ggaagcttca
     1561 ttagcgcttg gtgccacaag atggcaaaca atctacaaag tcgtgctacg agcagccgtg
     1621 cccgggattt taacagcagt cgtttttggt atggctcgtg cgtttggtga agccttagct
     1681 atccaaatgg tcatcggtaa tgcagcattg atgccaacag gacttactac tcctgcttct
     1741 acattgacgt ccatcttaac aatgggaatc ggaaatacaa tcatgggaac agttgaaaat
     1801 aatgtgttat ggtcattagc gctgatcttg ctattgatgt cattattctt taatatcgtg
     1861 atccgtatga ttgggaagaa aggagagctg aaataatgaa cgcaaaacgc tcagataaaa
     1921 ttgctacagg tgttttatat atagtttcgg ggatcatcgt attgatttta gcagctttac
     1981 tactttatat cttagttcgg ggaataccgc atatttcatg gtccttcttg accgaacctt
     2041 ccaaagccta ccaagaaggc ggtggaatcg ggatccagct atttaactcg atttatttgc
     2101 ttttgatcac gatgatcatc agttttccta tttctttagg tgcgggtatc tatctttcag
     2161 aatatgctgg aaaaaactgg ctaacagatg tgattcgcac gtcgattgaa atcttgagct
     2221 cgttgccttc tgtcgttgtc ggtttattcg gtttcttggt tttcgttatt caattccagt
     2281 atggtttctc gattatttct ggtgcattgg ctttgactgt attcaacttg cctcttttga
     2341 ctagaacagt tgaggagtct ttacaagcgg tccattacac acaacgagaa gcaggattgg
     2401 ccttaggttt gtcacgctgg gaaacagtga taaaagtggt ggttccagaa gcgacaccag
     2461 ggattttgac tggtgtgatc ctaagttctg gaagaatttt tggtgaagca gctgcattga
     2521 tctatacggc tggacaaagt gcacctgcgt tagattttac gaactggaat ccattaagtg
     2581 tttcaagccc gatcagcatt ttccgtcaag ctgaaacatt agctgtccat atttggaaga
     2641 tcaatacaga aggcacgatg cctgatggtg tttctgtttc ggctggcgct tctgccgtgt
     2701 tgatcattgc tgttttactg ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc
     2761 gaaaaatgac ttcagcctaa ggagaagaag caaaatgaaa aaatataact ggaatgaaac
     2821 aaatgtcatc accatgccaa aagagaaaga agtagcacta tatacggatg atctacatgt
     2881 ctggtatggc gacaatgaag caatcaaagg agtggatctg gaatttgaaa aaaataagat
     2941 cactgcactg attggtcctt ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg
     3001 aatgaatgat gggatcgcta atacgaaaat cactgggaaa atcatgtatc aaggcgtaga
     3061 tattaatgcg cctcaagtgg atgtttatga gatgcgcaaa cggattggca tggtttttca
     3121 acgtcctaat ccgttcagca agtcgattta tgacaatatt acatttgctt tgaaacagca
     3181 cggcgaaaaa aacaaaaaga aattagatga aatcgttgaa acaagcttga aacaagctgc
     3241 cttgtgggac caagtaaaag acagcttgga caaaagtgcc ttggctttat ctggcgggca
     3301 acagcaacgt ttatgtattg ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga
     3361 cgagccggca agtgctttgg accctatttc gacaggaacg gttgaagaaa cacttgtaaa
     3421 cttgaaagaa gactatacga tcatcatcgt gacgcataac atgcaacaag cagctcgtat
     3481 cagcgactat actgcctttt tctatctagg aaaagtaatg gaatatgaca aaactaacaa
     3541 gatatttacc agaccaaaaa ttcaggcaac cgaagattat gtgtccggtc attttggtta
     3601 agacggaggc ctcaacatga caaaagaaat tatttcatct aaagatcttc atctgtatta
     3661 tggaaagaaa gaagcactaa aagggatcaa tctaagtttc aatcaagggg aaatcactgc
     3721 gatgatcggt ccttctggtt gcgggaaatc cacttatctg cgttctttga accggatgaa
     3781 tgacctgatt ccgaatgtaa cgatcaccgg aagtgtttta tataaagaca aagacatcta
     3841 tggtccaaaa acagatgttg ttgaactaag aaaagaaatc ggcatggtat tccagcagcc
     3901 aaacccattt cctttttcta tctatgaaaa tgtcatctat ggattgaaat taaaagggga
     3961 aaaagataaa aaagtccttg atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg
     4021 ggatgatgtg aaagacaaat tgcataaaag tgctttatct ttatccggtg ggcagcagca
     4081 gcgtgtatgt atcgcacgtg ttttggcagt taatccggaa atcatcttat tggatgaacc
     4141 aacaagtgcg cttgatcctg tttctactgg gaaaatcgaa agtatgctgt tagaattaag
     4201 agagcggtat acgatgatca tcgtgaccca taatatgtct caagcttcac gtatttcaga
     4261 taagacggca ttctttttag atggtcactt gatcgaatac aacgatacga agaagatatt
     4321 catgaatcca gaaaaacaag agacagaaga ttatatttca ggtcgatttg gataaggagg
     4381 aaacggaatg ctacgtacac aatttgaaga agaattgctg aatctccata atcagttcta
     4441 cgaaatgggg atgatggtga gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca
     4501 cgataaaatc ttagcccaag aagttatcga aaacgatata aatatcaata acatggaaac
     4561 acgcttggag aagaaaagtt ttgaaatgat cgcattgcaa cagccagtta caactgattt
     4621 gcgtatgatc attacggtca tgaaagctag ctcggattta gaacgtatgg ccgatcatgc
     4681 ggtgtctgtt gctaaatcca cgattcgcgt aaaagggcag acgagaatac cggaaatcga
     4741 aaaagagatc tccgatatgt ccgattatgt gaaaaaaatg gtggacaacg tcctagtagc
     4801 ttatgtcaaa acagacgaaa aagacgcacg tacgatcgcc aatatggatc aaagagtcaa
     4861 tgactatttc aatcgaatct ataatgcaac aatcaaaaat atgcaggaaa atcctgagac
     4921 agtcatcagt ggaacagact acttgaatgt tgccagttat ctagaacgga tcggagacta
     4981 tgtgacaaac atctgtgaat ggatcgtcta cttggctaca gggaaaatca ctgagttaaa
     5041 tacgaaccac aatgagattt tctaaactga agctgtgatt atgccgtttg tttaggaaaa
     5101 gaggttgtga aatcagcggt tgtgaaaaag ctgttctgca tcaagtaata agaacagtca
     5161 aacaaaaaat agctcctcat atttttcgtt tggctggact tattaccgca gatgcaagct
     5221 tttgaacacc gtttattcgg gaaagggttt gtgacaaaac atgtcacaaa cccttttcat
     5281 catttcatag aaaggataaa gaatgcaaaa aaaaaattag catcatcact atatttttta
     5341 tttgtgtgct tacttcatgg gttattcagc atcaagctgc tttatatgaa gtacctgtca
     5401 tcgagatcga aaaagtacga ataaaagagg atcatcaatt gattacagga agactgatga
     5461 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
     5521 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
     5581 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
     5641 ccatgttgct tattggcgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
     5701 tcgttttact ttacttttgt tgctcatctt tactaaaaca gaagggattt ctctattaaa
     5761 tatatctatc ctgttcgtcg ttatagggat gattattagc atattggctt taaatggatg
     5821 gaatagaagc agtataatca aaatttcagc tgcagttacg agtgtttttt tagcattttt
     5881 tatttgtctt ttgacaatga atcattggaa agaccaagga ctgcgctatg aggaactgaa
     5941 ttatctgaca cgaccgtatc gttctgtttt tctttctagc gtactgattg gaacagctgg
     6001 aggaacactc gataccgtta tcacagtcat tgcgacatta gaagaattga ctaagcgaca
     6061 accagaaata acagcgaaac agttatggct ttcaggaagg caagtgggtc gagatgtgat
     6121 tggctcgatg accaatatcc ttttgttcgt ctatatcagt gggtcagttc catcgcttct
     6181 tttatattta ggaaatcaat ggtcgttcaa agaaacgata gaacagcatt tatcattgga
     6241 atttttacgc gtcattgctg gcagcttagg aatagtatta tcgatcccta tctcggtcct
     6301 cttttttctg actgtaagga ggctgaaacg atgaatgtac ttattatact tgttgggatt
     6361 ttagcaatca gcgtgctact tattggcggt acgcaaggta tgtatattct tttaggctta
     6421 ttcatcaatt tagggatctt tttcctgttg ttattcggtt atcatcagaa gtggccgata
     6481 ctggcattgt cggtcatagg atttttattg attgctgtgg ttatcttatt ttttatcaac
     6541 ggttataatc taaaaatgcg tgcagctttt gtgagtatac tgatcttttt attttgtttc
     6601 ctactattgc ttcctatcac tgatttttta gcgatccaag gttttacatc ggtagaacta
     6661 gaagaattat ctggcttaga taagacttta gcgattgatt ttagattact tacgagatcg
     6721 ttgcttctga ttagtcttag tggtgctgtt ttagatgcta gtgtagcaat ttctagtggg
     6781 acattcgaag tatatcaggc aaatccgcaa ttatcttttt accaattgcg ccattctagt
     6841 ttcgctattg ctaaaaaaat attggcgtca acagtaatga cacttctttt tgcattcatg
     6901 ggaagttctt tagcgttgat cctctggttt attgatttag acattccttt tcagcaagtg
     6961 atcaatgaaa aatcatttgt tctagaatat acgatggcta ttcttaccac attgtctgca
     7021 ctacttgttc ttcctttaac ctgcgtgact acagcatatc tctttacaaa aaagaaagaa
     7081 caactcaaga tggagtaaaa gttacaactt aagtcctatg acatttttac tgaatcagtg
     7141 cataatatgg atgtaaggaa aagcttgcaa acaattttgc ttaaggaggc aatttccatg
     7201 aaagaaagag aacgtatttt agatttagtg aaaaaaggaa tcctttcgac tgaagaaggc
     7261 ttagatttat tagaaagcat ggcaactgaa aaagacgaaa agcaaataaa aaaagaagca
     7321 gacaaggtaa ctgctagtca taaagaaaaa gatcaggcaa gtcaattgat cgataaactt
     7381 gaaaacggcg aagaagaaat ttctgagcca gttgatccaa aggaaagaga aagacaagat
     7441 caagaaaact tagaaaagat cttagatgaa ctagcaactg aagcgaataa aacttcggcg
     7501 cgtctagatg aagtaaatgc tcaattcgct gacaataagg cagcacgcaa tgagaaacag
     7561 gaagcattga tgcagctgaa tacaaaagaa gaattaggcg agttgacaga agaagaatta
     7621 gcacagcgtc agacgttaga agcagaaatc aaagaattag aagctagtgg cgatacactg
     7681 ttagaagaac aattgaaatt agaagcagaa ttaaaagata tccgtaagaa tcaatggtct
     7741 gagaaaaaag aaacgtttac tgataagttt gaattgccag atgattggaa agatcaagca
     7801 acagatacac tgaatcaagt aggcgaaaaa atgtcagaag ctggatcaca gttaggcaaa
     7861 ttcttgaaga aaacattcca aactgtttca gaaacagtga atgacaacat ggaatggaaa
     7921 gatgtcagct tacgtgtccc tgggattgcg acaacgaaat ttgagcatga attttactat
     7981 gaagctcctg cagcaagtat ccttgatatc aaggcagcaa acggaaacgt gacgttaaaa
     8041 acgtgggaca gcaacgatgt gaaagttgaa gctaagatca aactttacgg aaaaatgggt
     8101 gctgaaccat ttgaagcatt ctctgaacgg agccagattg aggttaatga agatcatatc
     8161 tccttccaaa ttccaaataa acgtgtgcgt gcagaccttg ttttctactt acctaaacga
     8221 gtatacgacc acgcagctat taaacttcta aatggcaaca tcatgattga aacattggaa
     8281 gctaaagata tttatacaaa atctacgaat ggtaacatca tcgtggacca attaactgca
     8341 acgatgctgg aagtagaagg tgtaaacggc aatatcgata ttcgaaacgg gaacatctta
     8401 gattctatta tcgaaacagt aaatggtaca gtgacatttg gtgcgacagc agaaaacctt
     8461 tctgtgtctt tggtaaatgg tgatgtacgt cttacgatta aagaagataa tttgaaaaaa
     8521 gtagaagcaa gttcagtgaa cggaaatgtc aaagtcgctt taccagatgg catcggtttg
     8581 gaaggacatg ctaaaacaag tcttggaagt atcaacagcc gtctgtccaa ttacgaagta
     8641 gttcgtgaga aaaaagaacg gacaaaccaa atgttgcaat tccgtcgtgt ttcagatggt
     8701 gaaatcacgc aagtgcaact ttcaacgaca acaggaagta tctatttaaa agatacagat
     8761 aaataaagag gttgtgaaag agatgttcag ccttgagcaa taagaagcga ttttgtaaaa
     8821 tagcttcccc tattttacaa aattgtgcct tattgccgat aggctatctc ttgaacaccg
     8881 tttaccaaga gattatgaac ctgccaatta tcgccgagtc acaaggaata atttcagaaa
     8941 aagctcgtca cgttttatga atatggtaat ttgtgaccga gaattttgca gaaagacacc
     9001 gattaagaag aaggttgtca caacgatttt cttcatttaa aatagtagaa cagaggtgag
     9061 acatgatgaa aaaaaagttg acaaaatcca ataaaaatgt tgttttgaca gggactttag
     9121 caggaatagg agaatacatc gggattgatc cgactgttat ccgtgtgctt tatgtattcc
     9181 tgagcttagt tgcttttgga agtccaatca ttttatacat tttattagct ttgattatcc
     9241 catctgcgcg ttctgcacga acaaactatg gacataacaa tgcatattat gaaaaaaaca
     9301 attatcgtga acaaactcgt gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg
     9361 atgacgaatg gagtgacttt taatgtcgta ctttcaacgt attttagtta atacgttaac
     9421 attcatatca ttggctgttc tttttcctgc gatggtccat gtgagcagta tttggacggc
     9481 cgttcttgcc agcttcgtat tgtctgtgtt gaatatgttt gtcaaaccga ttttgacgat
     9541 actgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta tcaatgcagc
     9601 gattcttaaa atgacctcct tttttgtggg agcagaacat tttagttttt cttcattcgg
     9661 tgcagcgttg ttagtagcta tcttgatgtc gcttgtcaat gtaattgtca gcgaacataa
     9721 cagtagaaaa taaaagaaag ctgtgccgaa ctatcggcgc agttttttta tttagaatca
     9781 ggaatgcgtt ttctattaca ttggtaatta aataaaaacg ttttttttaa ttggtagtac
     9841 ttttggctag acaatgataa aatgatagag aacactctaa tagaaatgag gtagcatgaa
     9901 tgtcagaagt tgtaaaagtt agccaattag tgaaaaaatt atctttggaa atcgtgacag
     9961 gcgacgagaa aagcttagac cgtgtcatcg taactgggga catctcacgc ccaggactag
    10021 agttgactgg ttattttaat tattattccc ataatcgttt acagctattt ggcagtaaag
    10081 aaatcagttt tgcggaacgg atgatcccag aagagcgttt gatcatcatg agaagattat
    10141 gtagtgaaga tatgcccgca tttattattt caagaggatt accggctcca gaagaaatga
    10201 tccaggcagc aaatgaaaat gggatagctg tactgcgttc tcccatctca acttctcgtt
    10261 tattaggaga attatccagt tatttagatg gtcgccttgc ccctcgaaca agtgtccacg
    10321 gtgttttagt ggatgtttac ggattaggtg tactgattca aggtgacagt gggataggaa
    10381 aaagtgaaac agcgttggag ttgatcaaac gcggtcatcg attgatcgca gacgatcgag
    10441 tagatgttta tcagcaagat gaattaacgg tgatcggaga accgcctaaa atcctggaac
    10501 atctaattga aatccgagga atcgggatca ttgatgtaat gaatcttttt ggtgcgagtg
    10561 cggtacgtgg ctctatgcaa gtccagttgg ctgtttatct ggaagcatgg gcaaaagata
    10621 agaaatacga ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa gtgggcattc
    10681 cacaaataaa aatccctgtc aaaacaggac gaaacgttgc aatcatcatt gaagtagccg
    10741 cgatgaattt ccgtgcacgt acaatgggct ttgacgcaac gaaaacattt gaagaaagat
    10801 taagtcgctt gattgaagaa aactcaggca acgactagca aggagtgaca atatgttagc
    10861 acaaatcaat cgagttgctt ttgagcttgt agggattcct atctactggt atgcattgat
    10921 catcgtatca ggtatcatta tcgccatgtg gcttagcagc agagaggctg ttcgggtagg
    10981 attaaaagca gatgatgtga cagattttat gttgtgggga ttgcctatct ccattattgg
    11041 cgctcgctta tattatgttt tatttgatct tccccagtat atagctgatc caatccaaat
    11101 attcaatatc cgttcaggag gcttagctat ttatggcgga ctgattgcag gcgcgatcac
    11161 gctgtatttt tttactaaat atcattttat ttctttgtgg acttttttag atatcgctgc
    11221 accaagtgta ttattagcac aggcaatcgg tcgttgggga aatttcatga accacgaagc
    11281 atatggacca gacacgacgc gaagcttctt agaaagtctg catttaccgc aattcatcat
    11341 tgataatatg tatatcgaag ggatctatcg tcaaccaaca tttctttatg aatcactttg
    11401 gagtttgatt ggatttattt tgcttttgtt tttacgaaag aaaccacacc ttttgaaaaa
    11461 aggcgaagtc cttttatgtt atctaatgtg gtatagtttc gggcgtttct ttattgaagg
    11521 attacgtaca gacagtcttt actttttagg tgtgatccgt atttctcaat tattatctgc
    11581 tgtgttattt gtcggtacat tgattctatt tatttggcgc agaaaaaaag gaaatttacc
    11641 ggattatgac cgctcattag gaaaaaatca acaaattatc tgatttttag agcgttaaaa
    11701 atgaaaaaaa tttcaggagt ggtaaaatga attatagagt agcggtttta ggaccaggct
    11761 cttggggtac tgctttggca caagtcctcg ccgaaaacgg acatgacgtt cggatttggg
    11821 gacatcgtgc ttcacaagta gaagaaatca acacccaaca cacgaatcaa cgttatttac
    11881 cagatcatta tctacccgat tccatccaag cattcaccaa tatggaagat gcagtaaaag
    11941 acgcagatgc ggtgctattc gtcattccaa cgaaagcaat acgttctgtt gcaaaagaac
    12001 tagtgcctaa attgaataca aagccagtga tcatccatgc aagtaaagga ttagaacagg
    12061 gaacacataa aaggatctct gacatcttgt tagaggaaat cccagcagaa aaaagaaaag
    12121 ctgttgttgt tctttctgga ccaagccatg cagaagaagt agcgaaacat gacatcacta
    12181 cgatcacagc tgcaagtaca gatgcaaaag cagcaaaata tgtacaagag ctgttcatga
    12241 ataattattt tcggatctat accaatcctg atgtgatcgg tgtagagatg ggggcagctt
    12301 tgaaaaatat tattgctatt ggtgctggcg ctattcacgg cttagggttt ggcgataatg
    12361 caaaggcagc aatcatgacg cgcggattag ctgagatcag ccgtttaggt gtagcaatgg
    12421 gtgccaaccc gctgaccttt atcggattaa gcggagtagg cgatctagtc gttacttgca
    12481 caagtgttca ttctaggaac tggcgcgcag gcaacttgtt aggacaaggg cagcccctag
    12541 aagaggtgct agccaatatg ggaatggtcg ttgaaggtgt gaatactacc caagcagcga
    12601 tggaactagc ccaaataatg gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc
    12661 tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat catgttacgt gatgggaaaa
    12721 aagaaaatga attctcatta acaatttttt gaaggagcga atgtatatga aagtcaaaaa
    12781 agcagttatt ccagcagcag gattaggaac aagattttta ccagcaacaa aagctatggc
    12841 aaaggaaatg ttgccaattg ttgataaacc aacaatccag ttcatcgttg aagaagcgtt
    12901 gaaatcagga atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga
    12961 tcactttgat tcaaatattg aattagaaac aaacctaagt gaaaaaggaa agaccgaatt
    13021 attgaaatta gtagaagaaa cgacagatgt caacttgcat ttcatccgtc aatctcatcc
    13081 aaagggttta ggacatgccg ttcttcaagc aagagccttt attggaaatg aaccgtttgt
    13141 cgttatgcta ggcgatgata tcatggaaga tgaagtacca ttgacacagc aattaatcga
    13201 tgattacgaa cgtactcatg cttctacgat cgccgttatg caagttcctc atgatgaaac
    13261 ttcaaaatac ggaattatca atccaggaga agtattggaa gatgggttat acaatgtcaa
    13321 aaactttgtt gaaaaaccag atccaagcaa agcaccaagt gatttagcta ttattggacg
    13381 ttacttattg actccagaaa tttttgatgt attgtccgag caacaaccag gagccggaaa
    13441 tgaaatccaa ctaactgacg caatcgatac attgaataaa acacaacgcg tatttgcccg
    13501 taaattcaca ggtaaacgtt acgatgtcgg tgacaaattt ggttttatga aaacaagtat
    13561 tgaatatggt ctaacacatc cagaagtagg tgcaccttta cgtgagtata tcctgtcttt
    13621 aggtaaaaca ttagaagcag aaggaagcaa gatcgatcat aatccaaaag gaataacaga
    13681 aatcaaagaa gaaaagaaaa aataaccctt ttttctaacg agcttcagct gctaattgaa
    13741 taaaaagtag ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc tatccttata
    13801 gaaaagagag acaagaaggg agtttgatca tgtcaggagg agaaattgca ggattgattg
    13861 cagcaatcgc ctttgcgata cttgtcgttt ttatcgttcg tgtgttgttg caagtttcta
    13921 aaactttaga aaaagtagat cagactgtag cagaagcaaa tacgacgatc gaaatcgtca
    13981 caaaagacgt agatcttttg tctagacaag tagaaggctt gctggtcaaa agtaatgaac
    14041 tgctcttgga tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg
    14101 atttaagtga aagtgtatca gaacttaatc attctagtaa aaacatcgcg acgaaagtcg
    14161 gcggaatcgg taaagcgaca gctaaagcga cggttgccgg aaaagtcggt gggacagcga
    14221 tgaaattttt taaaaacaaa aaacatactg aaacgacagg aggaaaataa aaatggctaa
    14281 aaaaggtgga tttttattag gtgcagtagt cggaggaaca gccgcagcaa tcgccgcgtt
    14341 attgttagca ccaaaatctg gtaaagaact tcgggatgaa ttagctgccc aagcggatga
    14401 tttgatggat atggcttcag attacacaga tatggcgaaa gaaaaatcga ctgagttaac
    14461 aggtattgca aaagaaaaag cgcaagatct ttctcaacaa gcaggtgatc tagcaggtag
    14521 tgtcaaacaa aaagtaagtg aatcagcaga tgaagcagga agtgaatctg acgatatctt
    14581 gttaaatctt aaagaacaat cagctgactt atctaatcgt ttcaaaaaaa cggcagaaga
    14641 tatgaaaaaa cctgcagatg aattaggaag tattgctaaa gatacttcag aagatatcat
    14701 gattgatgta aaagacgctg ctggtaaagc aggagatact gtcaatgaag gaatgtcaga
    14761 agcaaaacca gttgcagatg acacaaaaga aatgatgaaa atggcaaaag aagatgcgaa
    14821 agaagctaaa aaagaaataa aagatacaat gacagattca tctacaaatc actaaaatca
    14881 caaatttata gaataaaaag gtgggagcgg actagctctc acctttttta ctcttatttt
    14941 tctaaaacaa gtaattcttt tggtgtagtc gtgaatggca cacagccatc gcttgtcaca
    15001 tatacacagt cttcgatacg tacccctact ttttcaggaa tatagattcc tggttcaaga
    15061 gagaagcaca tcccttcttc gattactaag tcatttcctt ctacaaggga agggaattcg
    15121 tggacggttg ttccgatccc gtgacctaaa cggtgattga aatattcgcc gtagccatag
    15181 ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc tggacggacg
    15241 gcttcttgtg cggcaagctg tgcttcaaga gtgatgttgt atattttttc ttggaagtcg
    15301 gttggttttt gataagcaat tgtacgagta gcatcactgc aatagccatt ccaaacaacg
    15361 ccaagatcga ataacacaag ttcattcggt gaaatcttcg tgtttcctgg tgtaccatga
    15421 gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg tgaaacacct
    15481 tgttttttca attggtattc gatttctgca atgatcgctt gttcagtcac gccttctttc
    15541 actgctttaa agccaatctc aaatgcaaca tcggcccatt taccagcttc gatcagtttg
    15601 tctttttcct ctagagtttt gatcagttgt agacgttgga tgatggaagt tagatcagtg
    15661 taagaatccg ttcccgtcaa gatatcagag atagcatcga agcgctcgac agtcaaggcg
    15721 ttcttttcaa tccctacatt gccaggctga ccatagcgag cacgaagttc ttttccgatg
    15781 atctcccaag ggttttcgct gtctaaatag ccacggacat catacgccca actactgttt
    15841 tgagcatctt caacttccaa agcaggagtg aaaaggaaag gatcttgttc aagagggata
    15901 aaaagagcta gtacacgctc atgaggatca ctttcgtatc cggaaaagta agcgatgtgg
    15961 ccagggtcgc tgatataagc caattcgact tggtgttgtg ccatccattg acgtagttca
    16021 ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt attcaataat
    16081 tgtaccactg atttattaaa agaccaacgt tctaaaaact gaaaaaagta aaaattttca
    16141 tgaaaacaga gataaaacgg ttgcaattct tgagattatt tgatattcta ttttagaaat
    16201 gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa tggaaaagca
    16261 aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta ctgtttcacg
    16321 tgtcgtaaat ggcaatccaa acgtcaaacc agcaacaaga aaaaaagtct tggaagttat
    16381 cgatcgcttg gattatcgtc caaacgcagt tgcgcgtggc ttggcaagta agaaaacaac
    16441 aacagttggt gtaatcatcc ctgatgtcag caatatgttc tttgcttctt tagcacgtgg
    16501 tatcgatgat gtggcaacaa tgtacaaata caatattatc ttagcaaatt cagatggcaa
    16561 cgaccaaaaa gaagtcaatg tattgaataa cttactggct aagcaagtgg acggcgtgat
    16621 cttcatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt caaaaactcc
    16681 tgttgtatta gctggctcta ttgatccaga tgaacaagta ggtagcgtaa acattgatta
    16741 tgtatccgca acaaaagatg cagtaaacaa attggcaaaa agcggcaata aaaaaatcgc
    16801 ttttgtcagt ggtgcgttga tcgatcccat caatggacaa aatcgtctga aaggctataa
    16861 agaagcatta agcgaaaata acctaccata tagcgaagga ttgatctttg aagcacaata
    16921 caacttcaaa gatggtctat ctctagtcga tcggatccat aatagcggtg caacagctgt
    16981 ctatgtttca gatgatgaat tagccatcgg tattttggat ggtttattag accgtggcgt
    17041 gaaagtaccg gaagaattcg aaatcatcac aagtaacaat tcactactga cagaagtagc
    17101 tcgtcctcgt ttaacaagta tcacacaacc attatacgat ttaggggcgg tatctatgcg
    17161 cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta tcttgcctta
    17221 tggaatcgaa gaaaaaggat ctacaaaata ataaaaggac aaccattcag aggttgtgtg
    17281 cctgccgttt aactctgcgt tacaaggagc aatttcaaaa aacagcatct catgtttgat
    17341 gaatattgtg actcgtgatt cggagttggc aggagaacac cgtttatttg ggaaaagggg
    17401 ttatgacaaa attttgttat aatccttttt ttgtgtctta aaaaagagag aaagtggttg
    17461 aaattatatt tatcgtatat aaaacaatcc gtgtatagat gctgtatttt gttcaccatc
    17521 atcaaaaaaa taagggaatg tgacaatact attgtcacat tcccttatta ctgtgtattc
    17581 tattaaatca atcatctttc tcatctgatt ctttggttgc ttgattcgct cttgcataag
    17641 ctgaagcggt gttccagtaa tctgtatagt tttcatcggt accgccaacg ccaaacttaa
    17701 aggcgctttt cttcggtcca tttttcgccc atagcgattc aactgttttt cctggtgttt
    17761 ggatggattt tttattgaca gtaacttttc caggtgtttg accagtaaat ccagatactt
    17821 ttttcttcat cacatcagat gataattcga atttctcatc agtgccaaaa atgtcaggtt
    17881 ttgcttggta agcttgattg atcagattgg caagataatt agccgaacgt atccttgctt
    17941 tgctgtccat tccagtattg tcatcgtgcc ctgcccagct actgatggta atagacggag
    18001 tagagacaat tagccatgaa tctcggtatt cattggtaga accagttttt ccgacccaat
    18061 ctgcttttcc tagatttccg tttaagcttg agatggcatc tttgaatggt gtagtgattt
    18121 ttgcattgat cacactgcgc atcatgtcat tcatgatcga tgccgttgct tccgaataga
    18181 tccggacaga gttgctttcg tgtttataaa ttacatttcc tgcgttatct gtgatggaat
    18241 cgatgatata gccttcttca tagacgccgc cgtttgctaa agcctgaaat ccgttggtct
    18301 gttgaagcgt cgtgacatct actgtaccta aaggagcaga ttcaacgccc caattgtcat
    18361 ttgccgggta gttcatcttt gacaagtagt gctcgtaagc atattgtttc gatccaccgg
    18421 aattcaacac atcttgatat aagtgataag ccgggatatt gttggaccat tctagtgact
    18481 cacggacggt ttggaaggtg ttcgatcctt cgttagtagc attgacgatt ttctcgccag
    18541 cattttctcc ttcttgccaa gttgttgcat aatctgatac acgggattct gatccgataa
    18601 gccccatgtc gattgcaggt ccgtaaacaa gaactggttt gattgatgag ccagcttgac
    18661 gttctgtatc gaaggcatgg ttattttgat tctgactgta attccgtccg ccaacgaatc
    18721 cgtagatgcg gccagttctg ttatccatca atacatttcc ggtttctact tgactgccag
    18781 caccatcatc taacatgtat ccataatttt caacgccatt ttgcaatgca gcatagacat
    18841 ctttatcgat tgtcgaatgg attgtatatc ctttattttg gatcgtttgc tgtgcctttt
    18901 gataataagc gtcactgatc gatgaatcag acatatcagc gttgtctttt tcagccaatt
    18961 gttgtgcaat gattcttgtt gcttcattca tcacagtata atacaagaat tctctgtcag
    19021 tctgttctgc gatttgttgt ggtagaaagt cttttgtaag gtcataggct tttgcttctt
    19081 cgtattgttc tttcgtaatt tggccttcac gatacatact gaataaaaca aaatcttttc
    19141 tggcaaggcc ggcagaaaga tcttctttta gcgcacctgt gttcgtgtat ggactatacg
    19201 tgatcgggct ttggggtaat ccagcgatat aagcagcttg cggcagagta acatcttttg
    19261 catctacacc aaagattcct cttgcagctt cctggactcc agcaatgttt tgtcccttat
    19321 tattacgacc aaatggtgag acattcaaat acgtagcaat gatctcatct tttgaaaagt
    19381 atttttctac ttgtgcagaa agaatgatct cgtttgcttt acgtttgaat gttgtttcat
    19441 ctgtaaggat ctgctgtttg actaattgct gtgtcaaagt tgatccacca gaagagccaa
    19501 taccagtagc ttcagatact aatgctcgta aaacagcttt aggaacgtaa ccttggtgtt
    19561 tatcgaagta ttcatcctct gtgctgataa ttgcagtttt caataaaggt gaaattttat
    19621 cagatgaaat agtcgtgcgc atcagatctg tctggatttt tgatatctct gtattatctg
    19681 cataaacaat ctttgacgtt tctgtaatgt ttcctagttc tgtctgcagt gcttttttag
    19741 tcggcaattg tgtgtcttcg acgagatagg caaaatagcc agcgcctatt ccaataccta
    19801 gagctgcgaa catcaaaaga agaacagtaa taaaaacagt caatgattga aaaactctaa
    19861 aaataatctt aggaacaaac catttttctt tttttcttct attctgtttt tttcgagtat
    19921 ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt atagcaaata
    19981 atagaacaaa aaaacagaca aggctgtttt tggcaggaat ttctttttta aaggagttaa
    20041 aacgtgagaa aatgatcttt tttataaaag agttcttaag cttttcttta gagaaaattt
    20101 cagttactat ggagaacagg gcaaagaaaa atcaatcatt ttctttgtat atcatcttac
    20161 caatatatag gaattgttag taacaattgc ctttcaagga ggaatccatc catgttatta
    20221 ggctcacacg tcagcatgag cggaaagaaa atgctgttag gagcagcaga agaagcagcc
    20281 agttatggat cttcgacttt tatgatctat acaggcgccc ctcaaaacac tagaagaaaa
    20341 gccatcgaag agatgaatat tgatgccgga aaagcgtaca tggcagaaaa cggtctaaca
    20401 aatatcgtcg ttcatgcccc ttatatcatt aatttgggga atacgatcaa accagaaatg
    20461 tttccttttg ccgtccaatt tctgagagaa gagattgttc gtgcggaagc tttaggcgct
    20521 actcaaatta cacttcaccc aggtgctcat gtaggggcag gaacagcagc cggaattgct
    20581 agaattattg aaggcttgaa tgaagttttg acaaaagaac agacggcaca aatcgctttg
    20641 gagacgatgg caggtaaagg tacggaaatc ggtcgaacat ttgaggaatt agcgcaaatc
    20701 atcgatggag taaaattgaa tgaaaaatta tccgttactt ttgacacctg tcatacgaat
    20761 gatgccggtt ataatattaa agaagacttt tcaggtgtaa tggaagagtt tgataagatc
    20821 atcgggttag atcggcttaa agtactccat atcaatgatt ctaaaaatcc gcaaggttcg
    20881 cataaagacc ggcacgccaa tattggtttt ggaacaatcg gattcgacac gttgaataag
    20941 atcgtccatg atccaatgtt tgacaatatt tcgaaaatct tggaaactcc ctatgtcggc
    21001 aaagataaaa agaaagctta cgcaccatat ggaaaagaaa tcgccatgtt caaagcgcaa
    21061 acattcgatc cagaagtgtt tcctgagtta ttagcagaag aagtgatact ctaagatttt
    21121 ctatttgaca gcagcaaaaa aggaccaggc acattgcctt gtcctttttt gtttaagaaa
    21181 caaaggagta ctttttgcag tctttttcat catgcatctt ctcccccctc tttttaaatt
    21241 tagacaaaca gggtatactg gtagaaaagt aaaaaaacga aaagtttcac aagcaagtgg
    21301 caaaacgtga ttggaaggac gaaaagatgc taaacacttt aaaacccttg atcaaccaac
    21361 tgactaagaa acatacatac tttattcttg gcatagccct attatttaca attattcgtt
    21421 tgtatttata tagtaaagca atctatggaa cagacatgtc ggcaacctat gatgatcagt
    21481 tgctgattcg ctatgctcaa aatattttgg acggtaaatg gctaggggag tatacgacta
    21541 caacactttc caaaggaatc tcctacagct tatttttagt acttgcacat aaactgcatt
    21601 tttcttatgc agttttattc ggcatgttca gtatttttgc cagtatattg gttgcactcg
    21661 cgtttaaacc gatcgtgaag aacaagtatc ttcttttaag tatctatttt tattttttgt
    21721 tttctccgat tagttttaca catgaatatt ccactcgaac gtatcgtaat gccctcgtga
    21781 ttccggctgt tgagatcatt atcgcttgtt tattagctat ttattaccgc aaatacggtt
    21841 ctagcagaaa attgctgcca tggtttttag ggctttctgt cgcttttcct tttttttggt
    21901 tcattcgcga agattctatc tggcttcttc cttttactgt agtagctttg ggtatcagtt
    21961 tcgtccagat cttgctaggg caagactttt ctttcaaaga agtaaagaag ttaaaaaaac
    22021 gactctttct tctctcgaaa cgggaatggc tgaaactttc attgttttta cttcctatag
    22081 ttggcttttc catcgtctat cagtggatcg aatataaaaa tgagacaaca tacggtattg
    22141 caaccatcaa tgaccgttcg agtggagaat ttggacgttt gatgaaacaa ctgatccgaa
    22201 tagatgatga aacgaacttg aataaagaaa acagtgagat atgggtctca catgctgcac
    22261 taaataaagc attagccgca tctcctacat tccatactgt ttctacccgt attgacgaat
    22321 tatatacgac tcacgcgtgg actcaaggtg gaaaagtaaa agaattaccc ggagatatca
    22381 tcttttgggc attgcgagat gtagtaagcg aatctggtta ttaccaaaac aatgcaaaag
    22441 aaaccaatga tttttggaaa aaagtcaacc aagaattaga agaagcctat cacaagggaa
    22501 ccatcaaaaa gaaaaaagag ttctatctga tgggatcagg agatggaaag acgatgagtg
    22561 atctgccaat cgtattcgat ttccttcaat caggatacgc ttatgcatgg cgatataaag
    22621 atttccatca aggaggaaat tatagtttcg gaacgcaaga acaagtcaaa caggctcaag
    22681 aattattaaa ccttccgcta atggagaatt ggatcgatag taatgaaccg agaacaccta
    22741 acttccaatt aacaaaatca gcaaaagtcg cgaatgtcat cataaaaatt taccaagttc
    22801 tggctattcc cgtgctgatt gcttcaatag cagggatatt acttatgata ctaggaaccg
    22861 tatttgcaaa agctgatcgt gcctattata gttcctcttt gattgtggtc accggattaa
    22921 tactgacaca attggttttt cttttcggtg tctcatggtt ttgttcttat gcgccagaaa
    22981 aaaaggacta ttttttaagt gtctacactg gtgcaggcgt gccattaacg caattcatcg
    23041 tagtaacaag catgctagtt ttcagtaaat taaatctgaa aaaagcttta tcttctaaat
    23101 aaaaagaccc ttggtagcta gatttttaac ctaactatca agggtctttg tgtggaaaaa
    23161 tcagttttta ttcgatttct tctaccgttt cctgcaaaag ctgttcatat tttttgtttc
    23221 ctttatataa atcaccaatc gtccatgcca acaggagaag gacaagaaca atgattacat
    23281 aagcgatgtg atcagccaat gccagttgag cggaagtggc ttgctcgccg aagaaacttt
    23341 cgatctgtcc tggtaatcct cgtaagttca agtaagttag actaatcaca gagaaccagc
    23401 caagaagttt tacgatgaat gaatttttaa atcggctacc catctccgct tggctgttgg
    23461 tcatcatcag taaaggaatc atcgaaaatg gcaacgcaaa agccaagaac acctgggatt
    23521 cattcattaa gttattcaaa gcggtatgtt cttcgatcgt tccttttgta cttgtaaaca
    23581 aaacacaaat caatacagga atcacagaaa gcaatcgtgt gaccagtctt cttagccaaa
    23641 tcgggatacg catatggatg aatccttcca tgatgacttg accggtcaac gtaccagtaa
    23701 tagtagagtt ctggccagaa gcaagaagtg ctacagcaaa taaagtggac aagataccag
    23761 attttgctac agcaatcaaa ataccattgc tcatagctga tggatcagat aaagcttcat
    23821 acagaccaaa aaaggaagga tcttggacag aaccggtttt gaagacagcg acacccatga
    23881 tcaatagcag tgcattgact agaaaagcca ttgacaactg gatattagaa tcccaagtag
    23941 aaaaacgtac agcttgtgct acatcttctt cgttttggtg atcgatcttc cgagtttgtg
    24001 aaactgctga atgaagatat aagttatgcg gcatgactgt tgcaccaata atccctaaag
    24061 ctccagttag cggagtttcg ccaccaatga caggtgagtc tgagaatgta tggctgttag
    24121 ggataaagcc agcaaataaa ctgtgccaat ctgggttaga caagccaact tgatagacaa
    24181 aaacgaataa aatgacgaaa attaagcaga caaccagtgc ttcgatctta cgaaacccga
    24241 cttttgtcag tagtaagaga acgaaaacat ctaatacagt aataaagacg gcaatcacca
    24301 atggaatatc aaaaagtaag tagagggcga ttgctgcacc aattacttct gcaatatctg
    24361 ttgccataat ggctaattca gtcaagatcc ataaaatgac acctaatgtc ttacttgtgc
    24421 gtgctcggat tgcctgtgcc aaatccattt ggctcacgat tcctaatttt gctgccatat
    24481 actgtaaaag catagcaata agactagaca tcaaaataac tgacatcaat aaatattgga
    24541 agttttgtcc tccggtgatc gaagtagacc agttacccgg atccatatac ccaactgcta
    24601 ctaacgcacc aggtccagag taagcaagaa gtgttttaaa aaaacttttc ccctttggta
    24661 catcaactgt tccatttact tcagctaaag ataaaccatt tgtatgttca ataagtcgat
    24721 gcttatgaaa ttgtttattg gattgattat tcaagaaaaa accacctttc ctatttatct
    24781 acgattacat tctacacctc aaaaagaaaa aattaaagaa aaagattcgg tatgcctaat
    24841 ttattttcgt ggcgtgtctt gctagatgac gaaagaaatt ttgttctcct ctaaaaaaac
    24901 cgctattttt tgcatgaaac taaagaatac gcctttcttt tttcaataaa ctttagtaaa
    24961 ataagtcatt gagagcaatt cggaaataga aaggagagat ttatatgtta tcaacaggta
    25021 ttgtcgtact tattgctatt attgctttgc taatcggcgc agtaggtggc tttttcctag
    25081 cgcgtaaata tatgaaagac tatttagaaa aaaatcctcc cgttaatgag gacatgttgc
    25141 gatcaatgat gatgtcaatg gggcaaaaac catctgagaa gaaaattcgc cagatgatgc
    25201 agcaaatgaa gaaccaaggg aaaaaatagg ttctgaccca acgacttttt acgggctgat
    25261 tttttcagcc cgttttttat ttcgtaattt gttagactat tcagattatt cagcaaaaaa
    25321 gacaagcaca gacaatccta ggctcttgat aaagacaaag gaggagtaaa atgtcgattt
    25381 ttagaaaatt aggctggttt tttcgtcagg aaaagaaaca ttatttaatt ggagttttct
    25441 cgctattcgc agtggccttg gtacagttag ttcctccaaa agtgattggg atcatcattg
    25501 atgagattgc cgataaaaat ttatcaataa agatcatttt cgggtggatc ttcctattga
    25561 ttatcgctgc ttttttacaa tatctttttc gttatatctg gcggacgaat atttggggaa
    25621 gtgcagctcg gttggagaaa gaacttcggc agcagttatt caaacatttt acagaaatgg
    25681 acaatctctt ttaccaaaaa tatcggactg gggatttgat ggctcatgca acgaatgatt
    25741 tgaatgcgat ccagaatgta gcaggagctg gtatcttgac ctttgcagat tcagtgatta
    25801 cgggcggaac aacgatcatt gcgatgattt tgtttattga ttggcgctta acactgattg
    25861 cgatgattcc tctgcctttg ctggccatca cctcacgagt gctgggatcg aaactccatg
    25921 atgcttttcg tgattcgcaa gaagcttttt ctagtatcaa tgataagaca caagaaagta
    25981 tcactgggat caaagtcatt aaaacatttg gacaagaaaa agaagatcta gcggattttg
    26041 aagaaaagat tgaagatgca attattaaaa acaaacgagt caatttttta gactccttat
    26101 ttgatccatt cattaccttg attatcggtc tgtcttatgt tgcaacaatt attttaggtg
    26161 gaaagtatgt cttggaaggc acgatctcga tcgggcagat ggtctcattt attagttata
    26221 tcggtatgct tgtttggccg atgtttgcga ttggtcgatt gttcaacgta ttggaaagag
    26281 gaaatgcttc ttatgatcgc gtcaatgaac ttttgcatga aaaaacgcat atcgtggaac
    26341 aactgggtgc agtgcagacc ccggcaaaag gcgagctgaa tgtagctatt tcctctttta
    26401 attatctgaa agaagaatca ccagcattag aacaaatcaa gtttgcaatc caagaaggag
    26461 aaacattagg tattgttggg aaaactggtg cgggaaaaac gacgatcatc aaattactga
    26521 tgcgtgaata cgatgattat caagggaaaa tccaatttgg cggacgttcg atcaaagaat
    26581 attcattgaa tgctttatta ggatcaatcg gctatgtgcc tcaggatcac ttcttgtttt
    26641 cgatgaccat tcgagataat atccgttttt ctaatccaaa actttcagaa gaagaagtaa
    26701 agcatgcggc ttctttagca tttatcaaca atgaaatcaa ggaattacca aaaggttatg
    26761 ataccttagt tggagaacgt ggagtctctt tatctggcgg acagaaacaa aggatttcga
    26821 ttgctcgagc attgatcaaa aatcctgaat tattaatgtt agatgatgca ctttctgcag
    26881 tagatgccaa aacagaagaa gctatcttgc ataatttaaa agaaataaga aaagagaaaa
    26941 caactatcat tgcagctcac c
//
LOCUS       DAHHPA010000031        26815 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.032, whole genome shotgun sequence.
ACCESSION   DAHHPA010000031 DAHHPA010000000
VERSION     DAHHPA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.032"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(48..395)
                     /gene="rbfA"
                     /locus_tag="NBH55_001205"
     CDS             complement(48..395)
                     /gene="rbfA"
                     /locus_tag="NBH55_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCD1509201.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(422..2728)
                     /gene="infB"
                     /locus_tag="NBH55_001206"
     CDS             complement(422..2728)
                     /gene="infB"
                     /locus_tag="NBH55_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCD1509202.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2741..3052)
                     /locus_tag="NBH55_001207"
     CDS             complement(2741..3052)
                     /locus_tag="NBH55_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCD1509203.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3049..3342)
                     /locus_tag="NBH55_001208"
     CDS             complement(3049..3342)
                     /locus_tag="NBH55_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCD1509204.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3364..4539)
                     /gene="nusA"
                     /locus_tag="NBH55_001209"
     CDS             complement(3364..4539)
                     /gene="nusA"
                     /locus_tag="NBH55_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCD1509205.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4563..5036)
                     /gene="rimP"
                     /locus_tag="NBH55_001210"
     CDS             complement(4563..5036)
                     /gene="rimP"
                     /locus_tag="NBH55_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCD1509206.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5180..9532)
                     /locus_tag="NBH55_001211"
     CDS             complement(5180..9532)
                     /locus_tag="NBH55_001211"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCD1509207.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9744..11453)
                     /locus_tag="NBH55_001212"
     CDS             complement(9744..11453)
                     /locus_tag="NBH55_001212"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCD1509208.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11521..12789)
                     /gene="rseP"
                     /locus_tag="NBH55_001213"
     CDS             complement(11521..12789)
                     /gene="rseP"
                     /locus_tag="NBH55_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCD1509209.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12950..13750)
                     /locus_tag="NBH55_001214"
     CDS             complement(12950..13750)
                     /locus_tag="NBH55_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCD1509210.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13747..14559)
                     /locus_tag="NBH55_001215"
     CDS             complement(13747..14559)
                     /locus_tag="NBH55_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCD1509211.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14754..15311)
                     /gene="frr"
                     /locus_tag="NBH55_001216"
     CDS             complement(14754..15311)
                     /gene="frr"
                     /locus_tag="NBH55_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCD1509212.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15314..16036)
                     /locus_tag="NBH55_001217"
     CDS             complement(15314..16036)
                     /locus_tag="NBH55_001217"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCD1509213.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16172..17053)
                     /locus_tag="NBH55_001218"
     CDS             complement(16172..17053)
                     /locus_tag="NBH55_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCD1509214.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17152..17934)
                     /gene="rpsB"
                     /locus_tag="NBH55_001219"
     CDS             complement(17152..17934)
                     /gene="rpsB"
                     /locus_tag="NBH55_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCD1509215.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18293..18772
                     /locus_tag="NBH55_001220"
     CDS             18293..18772
                     /locus_tag="NBH55_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCD1509216.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18989..20080
                     /gene="phnW"
                     /locus_tag="NBH55_001221"
     CDS             18989..20080
                     /gene="phnW"
                     /locus_tag="NBH55_001221"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCD1509217.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20073..20858
                     /locus_tag="NBH55_001222"
     CDS             20073..20858
                     /locus_tag="NBH55_001222"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCD1509218.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(21204..22895)
                     /locus_tag="NBH55_001223"
     CDS             complement(21204..22895)
                     /locus_tag="NBH55_001223"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCD1509219.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23317..24264)
                     /gene="arcC"
                     /locus_tag="NBH55_001224"
     CDS             complement(23317..24264)
                     /gene="arcC"
                     /locus_tag="NBH55_001224"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD1509220.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24379..25398)
                     /gene="argF"
                     /locus_tag="NBH55_001225"
     CDS             complement(24379..25398)
                     /gene="argF"
                     /locus_tag="NBH55_001225"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD1509221.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25489..26718)
                     /gene="arcA"
                     /locus_tag="NBH55_001226"
     CDS             complement(25489..26718)
                     /gene="arcA"
                     /locus_tag="NBH55_001226"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCD1509222.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         7139 a         5795 c         4771 g         9110 t
ORIGIN      
        1 aaaaatcccg caacccctga tttcacagga tttgcgggat tttttgatta gtctttgttc
       61 aagtcgcgaa gcaattcatc gatatggttc ccatagacaa ctgattcatc aagttcaaag
      121 aacaattcag gtgtcttata aatacttaga ttgtgcccta actctcgacg aatcaatcct
      181 gatgctttat tcaatccatc ttgtgctttt ttcttatctg atgctaagtc agataaaatg
      241 ctgtaataga tcgttgcttg ctgtaagtcc cccgtaactt tcacatctgt gatcgttacg
      301 ttctccacac gagggtcacg gacctttttg cgcaaaatat cattgacttc ttttaagatt
      361 tcttgaccga ctcggcgatc acggtagtta gccataatga ttggcctcct tttatcagtt
      421 tttagtcatt tttgacttct tccatgataa atccttcaat tacgtcatct actttgatgt
      481 cattgaattt ttcaatcatc gcaccacatt cataacccat tttgacttct ttcacgtcat
      541 ctttgaatcg tttcagactt gcaagttggc cttcatagat tacgattccg tcacggatta
      601 cacggacacc gctatctcta cgaatatatc cttctgttac ataggcacct gcaatcgtac
      661 caactttaga aactttgaag gtttcacgaa cgatcatttg accggtgatt ttttcttcaa
      721 attcagggtc tagcatccct ttcatcgcag tttcgatttc ttcgatggct ttatagatga
      781 tacggtgaag acgaatatcg acttcttcag tatcagcttg cacttttgcc tgcggtgtag
      841 gacgaacgtt gaaaccgatg ataatagcgt tgctggctgc tgctaaggtt acatcacttt
      901 cattgattgc tccgacagct gaatggacga tttttacacg gacaccttcc acatcaatct
      961 tcttcaatga tgcagccagt gcttctgctg aaccttgtac atctgctttg atgattacgt
     1021 taacttcttt caattcgcct tctttcaggc tttcaaataa gttgtccaaa gtcacacggc
     1081 ttgtggcagc acggttttcc aataatgcac gttttgcgcg ttcttcaccc gctgcacgtg
     1141 ctgtcttttc gtcttcgaag actacgaaac ggtcacctgc ttgaggtaca tcattcaagc
     1201 cagtgatttc tactggcgtt gcaggtcctg cttctttatc gcgacggcca agatcgtttg
     1261 tcattacacg gacacgtccg taagtattac cgacaacgat tgggtcacca acgtgaagtg
     1321 ttccttcttg gacaagtaag gttgacacag gacctttacc tttatcaaga cgtgcttcga
     1381 tcactgttcc gattgcgcgt tgtgttggat ctgcttttaa atcttctact tctgcgacta
     1441 acaggatcat ttccagcaat tcttcgatgt tttggccgaa tttcgctgag atctcaacaa
     1501 aaatcgtttc tccaccccat gcttcaggaa ttaattcata ctcacttaat tcttgcataa
     1561 catgctgagg atttgcacct ggtttatcaa ttttatttac agcgacgatg attggaacgc
     1621 ctgcagcttt ggcatggttg attgcttcaa ctgtttgtgg catgacgcca tcatctgcag
     1681 caacaactaa gatcgtgata tctgtgatac ttgctccacg tgcacgcata cttgtaaagg
     1741 ccgcatgtcc tggtgtatcc aagaaagtga ttggtttacc atcaatatcg atctggtagg
     1801 caccgatatg ctgtgtgatt ccgccggctt cgccgcttgt tacacgtgaa tggcgcaatg
     1861 tatccaataa ggttgttttc ccgtggtcta cgtgtcccat aatcgttact actggcggac
     1921 gtgagactaa tttatctgga tcgacttctt ctgcctcgaa gaatttatcg atatcagcaa
     1981 tgtcaacttg gactttttct tgtggttcca tgccatagtc cgtcgctaac agttcaatcg
     2041 tatctttatc cagtgcttgg ttttgattga ccatgacacc aagcataaat aattttttaa
     2101 tgatttctgc tggctcacga tgaatttttt tcgcaatatc tgctacgttc ataccttcag
     2161 tatactctaa tacatctggc aattcacgga atttgcgtgg tggtacagct ggtttagttg
     2221 attgttgctg tttgcctttt ttaccttttt tattataacg attacggtta ttattattaa
     2281 atttgccgcg attgttattg tttggtttgt tgttattgtt agtacggttt tgattgccgc
     2341 cttggctcgt tccttgacga ttttggctgc tggtatttcc cgtattattt tggtttgttg
     2401 ttggtttttt tccttgattc acttgaccgc tcccttgacc gcgatcttga taactgcggt
     2461 tatttttatt tttttgttga ttgttcgttg tttctttttt acgttgatcg ttcggctgtt
     2521 gattggctgg ttttctgctt gcttgttgtg cagcttgttg tttcgtgctt gggtttttga
     2581 acgcttgttg caatttcttc tcatcactgc tagtaatcgc acccatatgg tttttcacat
     2641 cgatgccaag ttgttgtgct ttttccacga cgtctttact agattttttg agttcttttg
     2701 ctaattcata gattcttttg ttgcccatgc aatcaccttc ctaaccttca attagctcct
     2761 tggtcttctt cgcaaatccg gcatccatta ttccgaatac cttgcgaggt tttccgatca
     2821 tctgcgtgat ttctgcttca gaaaatagct caaagcaagg aacttcataa tatgaactct
     2881 tatcttttat cttttttctt gtattctcac ttgcatcggt tgccacaaac accaattttg
     2941 ctttttgttt gcggatttca gcaatggtca gttcttcacc cgtgattagt tttcccgcgc
     3001 gcatcgctag accgatgaga ttcatggctt tttgccgatt aactgtattc attttccaaa
     3061 taactcttta cgtgcttttt ggtgagtgac atagtcaaga agttcttgat aaaactcatc
     3121 tgttaatgtt gtttcgagta ctcgatcgag aattttttta tcccaagctt gttgtacttc
     3181 ttccggctca agtgagacat acgctcctcg tccaggcatt tttccagttg gatcgatcga
     3241 tacttcccct tcttttgaac gggtgattcg gaccatctct tttttaggtt tcatttcacc
     3301 tgaaaccacg gatttgcgta aaggaatttt cctttgtttc atctctttgc ctccttacta
     3361 aagttaagct tctggttttt cttcgaccgt ttcattgttg aatgcaattg tttcatattc
     3421 atcgtctgtt aaatctgact gaacgattgc ttcatcatgc agttcttctg tttctttttg
     3481 ttgttcttga ttttcataaa attccgtcat gtcagattct gatttgatat cgattttatg
     3541 gttagtcaat ttagcagcta aacgtgcatt ttgtccgcgt ttaccgattg ctagcgataa
     3601 ttggtagtct ggaacgacaa ctgtgcaggc ctttggattt gtctcatcga agatcacatc
     3661 catgacttgt gcaggattca atgcatttgc gatgaataca gctggatctt cattccattc
     3721 gacgatatcc atgttttcgc cttttaattc attgacgatt gcttgaactc gttgtccctt
     3781 tggcccgaca catgtaccga ctgcatcgat atttggatca tttgagcgaa ctgccacttt
     3841 tgaacgatcg ccagcttcac gggcgatgct gacaatttca acgattccat catagacttc
     3901 tggtacttct tgttcaaata agcgacgcaa taggtctgga tggctgcggc taacaaatac
     3961 ttgcggacct ttagacgtat tttctactcg ggaaacataa actttgattc gatcatgtgg
     4021 ttgataaaat tcatttggca tttgatcttg tttagaaaga actgcctcaa ttttaccaag
     4081 attgacataa atataacgtt tatcctggcg ttcgacgatt ccttgcatga tgtctttttc
     4141 ataagcactg aattcgttat aaataatcgt tctttctgct tcacgtacac gctgtaaaat
     4201 aacttgttta gccgtttgcg cagcgatccg tccaaaatct tttggtgtta cttcaaaacg
     4261 gatcgtatca ccgatctcat atgctggatt gattaaaagt gcatctttca atgagacttc
     4321 caactgtgaa tccatcactt cttccgttac ttcttttacc gcatagacat ggatctttcc
     4381 ttttttctga tcaaattcca cttcgacgtt ttgtgcttgg ccatagtgac gtttgtatgc
     4441 agaaaccaat gctgcttcta aagcatcgat cacgatttct tttgagatcc ctttttcagc
     4501 ttctaaagcg tctaacgcat ttaacatttc tttactcatt tttttctgat tcctctccgt
     4561 gattaaaatt ggatagctaa gcgagctttt gcaatatttt ttcggtcaaa agtcaattct
     4621 ttttctcgtg tttttatgcg tattttcaat gtaagctgtt cagcatcaaa tgattgaaga
     4681 aatccttcat attgtttttc gccatctact ggttggtata aggagacatg gacataatca
     4741 ccaagtgcct tttcatagtc gctttctttt ttcaatggcc gctctgctcc cggggaggat
     4801 acctccagga aataagcttg aggaatcgga tctggatctg ctgcatcaag tttttcactc
     4861 agtttctcac tgacaaaggc gcattcttcg atatcgatac cgccttcttt gtcaataaat
     4921 actcgtaaga accagctttt tccttctttt acaaattcga cttcgacaag ttcaaaattt
     4981 tgttcatcta aaatcggtgt caccatttct gtgactgttt caacgacgct actcaaaagt
     5041 ttcgcctcct taaaaattaa gtgcgtaatt ccattaaaaa gagtgagcga aaaatccgct
     5101 cactcacagt tagtatcatt acctataaga actataccat actctgctta aaattacaag
     5161 cagacaggaa aacagatgct tatagcatat cgaataacga taattgattt tcatctggta
     5221 gatctttcaa tacgccattg tcgttcatat attcgatcaa tgtttttgaa actttaccgc
     5281 gggtggcaag atcttctttg gataaaaatg gaccgtcttt tcttgcttca acgatttgtt
     5341 tcgccacgtt ggttcccaaa cttgggacag ctctaaatgg tgcgatcaac gtatcacctt
     5401 caataacaaa gttaacggca tccgatttat acaaatcaat cattccaaat ttgaatcctc
     5461 gttccagcat ttcattggct aactccagca ctgtcagttg attcttttct tttacggaag
     5521 catctaatcc cttgtccgtg atttctttca ttgcttgttt cacagcatct tttcctttgc
     5581 acatggagac tagatcaaag tcgtctgcac gtacagaaaa gtatgcacag taatacaaaa
     5641 tagggaaata aaccttgaaa taagcgacac gaagagccat caatacgtaa gctgcagcat
     5701 gggctttcgg aaacatatat ttgatttttg aacaagaatc gatataccag tcgggcacat
     5761 tattttcttt catcgccgat agataggttt ctcgcaattc atcagggatc ttgttccatt
     5821 gtcctttacg aacagtttcc atgattttaa atgccatccc gctatctaat ccagcatgga
     5881 tcaaataaac cataatatca tcacgacagc cgattacttc cgccagtgtc gcatctcctc
     5941 gtcgaatcaa ttcttccgca tttcctaacc atacgtctgt accatgagat agtccggaaa
     6001 tttgcagtag ttctgcaaaa gttgtaggat gcgtttcttc taacatccct cgaacaaaac
     6061 gagtgccgaa ttctggaata cctaatgtcc ctgttttgga ataaatttgt tcctgactta
     6121 ctccaagtac ttcaggaccg gcaaatatcc gcatcacttc cgggtcatcc gtagggatcg
     6181 tttgtgggtc gatccctgat aaatcttgaa gcatccgaat aacagttggg tcatcatgcc
     6241 ctagaatatc taatttcaat acattatcgt gaatcgagtg gaaatcaaaa tgagtcgttt
     6301 tccattctga attttgatcg tctgctggat attggatcgg cgtaaaatca tagacatcca
     6361 tataatcggg aataacgatg atcccccctg gatgctggcc ggttgtccgt ttgactccag
     6421 tagcgccttt tgctaatctg tcgacttctg cacttcgaaa ctgcagatta ttgtcccttt
     6481 cgtatccttt tacatagcca tatgctgttt tgtcggcgac tgtaccaatc gttccggcac
     6541 ggtaaacata gtcttcgcca aacaatactt tcgtatagtt atgggcttct gcttgataat
     6601 cacctgagaa gttcaaatca atatcgggca ctttgtctcc atggaatcct aaaaatgtct
     6661 caaatggaat atcatgaccg tctttgttta agcgtgcacc acattttgga cattgttttt
     6721 ctggcatgtc aaatccagaa ccatacgtac catcttcaaa aaactcggaa tattggcact
     6781 ctggacaata ataatgaggg gctaatggat taacttcagt gattcctgtc atcgtcgcaa
     6841 caaagctaga gccaacagag ccacgtgaac ctactagata gccatcttca ttacttttat
     6901 gcactagttt ttgggcgatc agataaataa ccgagaagcc attaccatta atagaattca
     6961 actctttttc taatcgtttt tgaatgatct caggtaacgg atctccgtac atttgttttg
     7021 cttttgtgta actcaattct gatatttcat cttctgaacc tggaatcttc ggtgtataaa
     7081 gttcatcttt gactggaatc acttcttcgc agatatctgc gattttattc gtattttcta
     7141 ctacgatctc tttcgctgtt tcttccccta aaaagtgaaa tgcagttagc atctcatcgg
     7201 ttgttcggaa atggacatcc ggcaaactat gacgattcaa cggattcgct ccgcccatag
     7261 aattgatcaa aatttttcgg taaatggcat cttcttcgtt cagataatgt acattacctg
     7321 ttgcaacaac gattttatcc aaactttttc cgatttctac caaattttgg ataatttctt
     7381 ccaaatcatg ttcatttttt accaattctt gctcgatcaa aggcgcatag accgcttttg
     7441 gcataacttc aatataatca tagaattttg ctcggtttct tgcctcttct accccttttt
     7501 gcatcatggc ttcgaatatt tctcccttat cacaagctga gccgatcaat agattttcac
     7561 gcatcttttt caattgggag cgtggtattc gtgggacgcg ttcgaagtat tcgacattcg
     7621 acattgaaat cagtttgaac aaatctttca gtcctgcctg attttttgct aaaatcgtta
     7681 catggaaagg tcgagccctt ttgtaagaat ctccttctcc gatatgctca tttagctgat
     7741 catgataaag catgttatga ttgtccattg cctctttcac aaagatccat gctaagtgac
     7801 cagttgcttc agcatcatag attgctcggt gatgctgttc caagctaaca ccaaactttt
     7861 tagataacac acctaatccg aaacgtttga attgaggata cagatatcta gctaattcca
     7921 acgtatcaat gactggattg gccgcttcag gaataccata ccttgcgtag ctagtattca
     7981 agaaccccat atcaaaagcc gcgttgtggg ctaccaaaat tgtatctttt gaaaattcaa
     8041 ggaacatccg caatacttct tcttccgact ttgaccctcg aaccatttca tccgtgatac
     8101 cagtcagatc taccgttgtt cgtgacaatg gatgtcctgg atcgataaat tcatcaaagc
     8161 tttcgatcac atttccttta tacattttga ctgcggctag ctcgatgatc gtgtcatata
     8221 ctgcagacaa tcctgtcgtt tccacgtcaa atacgacgta tgtggcttca ctcaatgctt
     8281 catgtgcatc attataagca attgggacac catcatctac gacattcgct tccacgccgt
     8341 ataagatttt cacacctgct ttttttccag cactatgtgc ttccggaaag gcttgcgcac
     8401 caccatggtc tgtaatggcg atagctcggt gtccccattt tcctgcttgt gccacaagat
     8461 cagagatgct gtttgttgca tccatcgtac tcatattgct atgaacatga agctctactc
     8521 gtttttcccc ttcaggagca taatctttgc gaggtgtatg tttgacttct atgatatctt
     8581 gcgcattcat gaccaaatca cggacaaacg tatcttcttg aatacttcct cgtaccttta
     8641 accagctacc gacactgatt gcatcaaata cttgttcatc tttttcgccg ttcgagaatt
     8701 ttttgacgat aaatgatgaa gtataatctg tgattttcaa agtcaagatc ttgcgttttg
     8761 aacgtagttc tctgacttct ttgtcgaata cgtacccctc aagtgtgact cttcgttctt
     8821 cttcaacgat actgatcatt ggtgtcgttg gttcatcggc tggaatattt cgtcctagct
     8881 ggatgggtcc ttcaagagct ggcgattgct cttttctttc ctttttcttt tgttcgtgga
     8941 caactagact ttcagctgct tgcttcataa aggcttctgc ctgttcttgt ttgcgttctt
     9001 caaaaagttt caatacacgt tccgcttgct gctcatccac ttctggttcg atacgaaatt
     9061 ttgggaatcc gtaactgacg aacaactctt ctactaatgg caagtattgt tgcttcaaat
     9121 aagaaatagc tcctgtactg tcgattggca agatgacttt acgatctttt atcacaggta
     9181 cctgcgtttt caatacttgc tgtgctagtg gtgtatcgca ttgctgattt tccaaagctt
     9241 gggcccagta atcttgcaag agctgttcat cgaatgtctg gtcatctgct aagatattca
     9301 gtttgacttg tgcaatatct ttaaaagcga tagtcagatg ctgagtcaat gaccgatata
     9361 gcataatagg caaaattttg gaaaaatgta gcgtaaattc ccataaacgc gatttacgat
     9421 ggaccactac actatccatt tcaccagcag taagcagtgg atgatttttt tctgcttcct
     9481 ctagttggat ttgttccaac agtttttcaa ataaatctcg tttttctgac aaagatatac
     9541 gccctttctg atgagaaaca atgcaattga tcgcatctta tcctcttctt tttcagtacc
     9601 ttttagtata ctcgtttttt ctgagtttta cattaaaaaa ttggaactgc gattcaaatc
     9661 gcagttccaa ttttaaaagc gaatcatcag attgtcaaaa acagctgatt cagctatttt
     9721 tattttctgt gttttaacgc ttattattcg ccttggttca agaggattgg taaagtattc
     9781 gccaattctt ctttacgtac ttcaaccatt tcgccagttt tcttgatttt cacttctacg
     9841 attccatcca ctgctttttt accgacagtg attctgattg gacagccaat caaatcagaa
     9901 tccgcgaatt tcactcctgc gcgttcattt ctgtcatcaa ccagtacttc atagccagct
     9961 tttgtcatcg cttcttctag ttcattcgtc aggcttgttt ggtattcgtc ttttagattc
    10021 atttggacga tatgcagatc aaatggcgca attccttttg gccaattgat tccttgttca
    10081 tctgaatgtt gttctacgat tgctgataat aaacgactta caccgatacc ataacagccc
    10141 ataatcacaa atttttctcg tccattttcg tctagtacag ttgctcccat agattcactg
    10201 taacgagtgc cgattttgaa tatatgtccg atttcgattc ctcgagtaaa ggcaagaaca
    10261 ccgttgttgt ctggtgaagg atctccttct tgtacaaaac gaagatcttc ataagaaagc
    10321 acagtgaaat cgcgttttgg attcacattt ttatagtgga atcctgtttc gttcgctcca
    10381 gtaatggcat tcgctaaatc ttggacataa cggtctgcat atacttttac gtcatctgaa
    10441 acaccgactg gtccgacaga accaaactca gcacctagat atttttgtgc ttcttcttct
    10501 gttgcttctt ctaaaaagtc agcagataaa aagtttttca gttttacgtc attgacttca
    10561 tggtctccac gtaccaaaac aaggacaggt tgttcatccg caatgaacaa tacagacttg
    10621 atgatttttt gaggttcaac agaaaagaaa tctgctactt cgtcaattga ttttacgtta
    10681 ggtgtagcta ctttctccaa ttcaagctgt gtttcatgcg atttttttga tacatagtag
    10741 cttgtagcca tctccaagtt tgcagcataa tcactttcag tcgaataaca gatcgtatcc
    10801 tccccgattt ctgagatcgc catgaattcc tttgagtctt tcccgcccat tgcgccgccg
    10861 tctccgatga tcgagcgaaa ttctaagcca caacgcttga atacttcggt ataggctttt
    10921 tcgtaatctt tatatgtctc gtccaaactt tcttccgttg catggaaaga atatgcatct
    10981 ttcatgataa actctcttcc gcgaagcaaa ccgaagcgag gtcgtttttc gtcacgatat
    11041 ttaggttgga tttgataaag atttaaaggt aatttcttat atgaattgat ttcattacgc
    11101 atcaaatcag taaaagtttc ttcgtgagtt ggtccaagga tcatttttcg atcattgcgg
    11161 tctttgaaac gatacaagtt tggtccatat gtttcgtaac gcccggattc ttcccaaagt
    11221 tctgctggca agatggcagg catcagcatt tctaccgctc cgattttttc aaattcttcc
    11281 cgcataatgc gtttcagttt ttccaataca cgatttgcta atggcagata tgaataaata
    11341 cccgctgata cttggcggat atatccagct cttagcaaga tttgatggct taatacttct
    11401 gcatcatttg gaacttcgcg taatgttgga atcaacattt ttgattgtct cattctatat
    11461 actcctttat ttcctcaaaa tagttatcgt ttgttattat accctatttg tcaacttttt
    11521 ctagaaaaag aatcgttgaa tatcgttcca tgtaactagc accatcaaca acattaaaaa
    11581 tccaaagcca gctaatgtca atattccttc tttttcttga cttaatggtt tcccgcgaat
    11641 cccttcaaaa atattcaaga ctaatttccc gccatctaag gcaggaatcg gaagcagatt
    11701 gacgattccg agattcattg aaagaagcgc catcaaacta ataacagttg ttacaccttg
    11761 atttgctgct tctgaagaca attggtacat catgacaggt ccgcctaatt tatctaaact
    11821 gaaacctgta aatagtgatc ctaaggcttt gaaaatttcc aatgaaccgc tgaaagcttg
    11881 acgcgtccct ccaatgatct tatccataaa acctgtattc attggggctt ttataccaag
    11941 ttggccaact ttttcgccat ttgactctac agatttcggt gtcacgtcga cagattgcac
    12001 ctgaccttct cgttcgatct taaagtctaa tgttttacct ggatttttcg taatgactgt
    12061 tgtcagatca ttccatgaat ggatttcttt tccgtccaca ctgactacct catcattttc
    12121 ttttaagcct gcttctgccg cagctccatt aggcatgatc tctcctacgc gattagtgtt
    12181 cgtcacttga acaccacctt gcataaatgc cagaatgatg aataacacga ttgccaaaat
    12241 aaagttattc atgggtccag caaagttggt cagcatacgc tgccacaatt tagccgattg
    12301 gaactgtacg tcttttggcg caattctgat ttctgttccg tcgttttcaa taatcgtcgc
    12361 atcgtgatca acagggtagg ttactacttc tgtttcgtct ccattcacat aaccagtgat
    12421 agtcagctca tcttctaaat cataacggct caattccatc gggatagcat ttgtcaattg
    12481 gattttctta cttaaattta tcttctctac gatgccgtca gaatttagta aaagagaaag
    12541 aggcattcct ggtgccattt ctgtttcatc atccccattg cctgccattc gaacataacc
    12601 gccaataggt aataggcgaa gcgtatatgt tgtcccgtct tttgcttgat gcccataaat
    12661 tttcggaccc ataccgatag caaattcgcg gacgaggatt cctgatcgtt tcgcaaagaa
    12721 gaaatgacca aactcatgaa caatcactaa tataccaaaa acgatgataa atgtcagaat
    12781 cgttttcata gcttttttct cctttacttg ttttttgtgt tttttataaa acaacgataa
    12841 aacactataa ttttaccata gtataccaaa gacaccaata gtatcaatat aatgaaaaat
    12901 aaaaacctgc gtcttttatg aaattcacaa agaaacgcag gtatacttac taaataatgc
    12961 caaataaatg catgatagga aatacaaaca gcaaactgtc gaaacggtct aagattccac
    13021 catggcctgg taagatattt cccgagtctt tcacatcata atgacgtttg attgcagatt
    13081 cgaccaaatc accaaattgt ccgactgctg aaaaaataat cgtcatcaga agcatgacaa
    13141 acataccatg attgaatagt tcttttgctg ggtatagcaa taaataaaga aatgcgacta
    13201 caacagcact accgatacca ccgagcgcac cttcaatcgt tttgttgggc gaaatatcag
    13261 gacttaactt atgctgacca taacgtcgac caaccatata tgctccgata tcagttgccc
    13321 aaacaataaa tagaccaaat atcaaaacag ccaagctttc tgttcgtgca ttgacaaaat
    13381 tctggaaacc tattcccaca tacaagctga caagcacagg aaaacctgct tcatcaatcg
    13441 tatacgtatt tttagaaata actgatgttc ctaaaagaat cataactata agataaaata
    13501 acataaatgt acttacattc tctggaagaa agaaaaacca tctttctttt ggcaaaacaa
    13561 gtaacactgc accaacggcg gataaaactc cttcaaaact taagagtgtc aatcctttca
    13621 tccggaacag ctcgtaaact cctactaccg ctaagattgc tgctgtaagc tcaaccgcta
    13681 tgcctccgat ccatataatc ggaataaaca agactagcgc cacagctgct gtgatcacac
    13741 gttgtttcat gactgatccc ctttctcatc ttctgttttt tttacaccgc cgaatcgacg
    13801 gtctcgattt tgataagagg cgattgcttc ttcaagatgc gcaccatcaa aatcaggcca
    13861 aagcgctttt gtaaaataca gctcactata tgcgatctgc cataacagaa aattactaat
    13921 tcgctcttct ccacttgtac ggatcatcag ctcaggatct tgtaactctt taggcaagaa
    13981 acctgtcatt aaatgcttag caatcaattc atcatcgatt tcttctgcgt gtatttctcc
    14041 ttttgctact tcttcggcaa tttcttggac agcagtcacg atctctgctc tacttccata
    14101 attcaaagca aaattcaata ccattccagt attgtttttt gtttgctcaa ttgcacgtct
    14161 tacagcatct tgcgtatgtt ctggcaatac gttttcataa cccatgacat gaactttgac
    14221 attttcttta atcaattctg gtacgaacgt atcaaagaag tcgacaggta gttgcatgag
    14281 aaaacttact tcatcttttg gtcgcttcca gttttcagtc gaaaaggcat aaagcgtcaa
    14341 cactttcact ccaagccttg atgctttttt tgtgactttc tttactgttt ccatgccttc
    14401 tttatggcca gcaactcgtg gcaaacgacg gttttgcgcc caacgaccat tgccgtccat
    14461 gatgatggca atgtgctgag gaattggtcc atcggtatta aattgaaatt cgcttttttc
    14521 ttgaatatat ttgttttttt gcggaaaaaa acgtaacatt ctttttcctc ctatacttaa
    14581 aagattccca ctatcttaag tattatagca atatctaact gtttttccta tgaaatcatt
    14641 gtttttttat ttaaaaactg aataattttt tcttttttta atttttaaat aaaagaaata
    14701 taaataaaaa cagaggaagc ggaatgatcc gcccctctgt ttttatgatt ttattaaact
    14761 tccaataatt ctttttcttt atcagcagcg atcttatcta cttctttaat gctgttatct
    14821 gtcaatgttt gtacgtcttt ttctaatccg cgcaaatcat cttcggtaat atcaccattt
    14881 ttttgttgtt ttttatattc atcgatcgca tcacgacgga tgttacgaat cgcgattttt
    14941 gcattttctg cttctttttt cacatcttta gctaattctt ttctgcgttc ttctgtcagt
    15001 tgcgggataa ctaagcgaat gacatttcca tcattggttg gactgattcc gatatcactt
    15061 gccataatag ctttttcaac atcttgaaga atgctcttgt caaaaggtgt gatcattaaa
    15121 acacgtgctt ctggaatttg gattgatgcc atttggttca atggtgttgg cgcaccgtag
    15181 tagttcacag taatacggtc caaaaggcta gcgttcgcac ggcctgcgcg gatttgccca
    15241 agttcacgtt gaagattttg tgctgctttt ttcatttttt cttttgcttc tgtcataatt
    15301 gcatctgcca tttttatttc ccccttacag ttgttccgat attttcgcct aagattgcac
    15361 ggcgaatatt tccgtgttca tttaaattga agacaagcag tggaatatca ttatccatgc
    15421 ttagtgaact tgcagttgaa tccatgactt gtaatccttt ggcaataact tccagatgag
    15481 tcagttcttc aaattttact gcatttgcat ctactttcgg atcagcagaa taaacaccgt
    15541 ctacattgtt tttagccatc aggataacat ctgcgccgat ttctgccgct cgtaatgctg
    15601 ctgttgtgtc tgtcgagaag tacgggttgc cggtaccgcc tgcaaagata acgatccgtc
    15661 ctttttctaa atgacgttct gctttacggc gaatatatgg ctctgcgatt tgacgcattt
    15721 caatcgaagt ttgcacgcgt gtcggtacac ccacgttttc caacgtatcc tgtaaagcta
    15781 aggcattcat aactgtagct aacataccca tgtaatcagc ttgagcacgt tccatgccca
    15841 tatgtgctcc gatttgtccg cgccagatat ttccgccacc cacaacgatt gccatttcaa
    15901 cgcccagctc gtgaacttct ttgatttctt ggatgatttc tttgattact ggtggtttga
    15961 ttccaaatcc tgcatcccca gctaaagctt ctccactcaa ttttaagact acacgctgat
    16021 atttcggtgt gaccatgttc attcctccaa tattttctat tgccatttta ccatacttag
    16081 atgatttcga cactctaaga ctggtttttc ttgttttgct taaaaaaagg gagcgcacaa
    16141 accgtgcgtt cccgtctgtt attcaacaga attatttttt cacttggctc ataacttctt
    16201 ctacaaagtt atcttcgcgt ttttcgatac cttcgccaac ttcgaaacgt acgaatgttt
    16261 taacagttgc gccttttgat gcaacgtatt tttcaacagt catatcagga tctttaacga
    16321 atggttggtc caccaaagcg atttctgctt tgaatttctt caagcgtcct tctaccattt
    16381 tttcaacgat attcgctggt ttgccttcgt tcaatgcttg ttctgtaaga acagtttttt
    16441 catgttctaa ttcagcttct ggaatttgtg attcgtttac ataacgagga ttgatagctg
    16501 ctacgtgcat agctacgtct cttgcaactg tttcgtcagt tgttccatct agaacagtca
    16561 atacggcaat acgtccgccc atatgcaagt atccaccaaa tgcagcattg tcgtcttttt
    16621 ctacaacttc aaagcgacgg aagctgattt tttctccgat aacttgagtt gcttcgatca
    16681 agtcagattc aattgtacct ttatctgttt tgattttcat tgcagcttcc atgtctgctg
    16741 gtttgttttc agcaacaagt tctgcaattt cttttactaa atcttggaac atttcgtttt
    16801 tagaaacaaa gtctgtttct gagtttactt caacgattgc tgcagtgtta cctttcacag
    16861 caactgaagc caatccttca gctgcaatgc ggtcattttt cttcgcagcc tttgccattc
    16921 ctttttcacg taaaagatca actgcttttt ccatgtcgcc ttctacttca actaatgctt
    16981 ttttcgcatc catcattcct acgccagtca tttcgcgtag ttcttttacc attttagctg
    17041 taacgtctgc catgtttaac tcctcctgtg tgtttttctt tttaaaaaaa gctgcttcaa
    17101 aggagagtga ggcttgtcgc ccagcctttg aaacagctcc atcattcaaa attattctgc
    17161 tgaagaattg tcgccttcaa caacatctac gatttcttcg attgatgttg ctgtgttttc
    17221 ttctgtgaaa tcttcttcaa ctacttgatc ttcaccttgg tttccttcga tgaaagcatc
    17281 agccattttt gaagtaatca atttaacggc acgaattgca tcgtcgtttg aagggattac
    17341 tacgtcgatt tcatctggat cgcagtttgt atcaaccata gctacgattg ggatatttaa
    17401 tttgtgtgct tcttgaacag caatacgttc tttacgtggg tcaacgatgt acattacatc
    17461 tgggattctt ggcatatcag cgataccgcc taagaatttt tcaagacgtt cacgttcttt
    17521 gttcaaacca gcaacttctt ttttaggtag aacttcaaaa gttccatctt cttccattgc
    17581 attgatttgt ttcaaacggc tgatacgttt ttggatagta tcccagtttg tcaatgttcc
    17641 gcctaaccaa cgatggttaa cgaagtattg tcctgcacga gttgcttctt ctttgattgc
    17701 ttcttgtgct tgtttttttg tacctacgaa taatgctacg ccgccttctt cagcaacgct
    17761 cttcatgtaa tcataagctg catctactaa tttaactgtt ttttgcaagt caatgatgta
    17821 gattccgttt ctttctgtga agatatattt cttcattttt ggattccagc gacgagtttg
    17881 gtgaccaaag tgtacgccgg cttctagtaa ttgtttcata gaaattactg ccatgtttgt
    17941 ttcctccgat ttggttttat ttttcctctt cttatcttct accttgccgg caaacttttt
    18001 caagcaccta ccgacaatcc aataagaatg tggatttgct gatgcataaa gcaccctcag
    18061 ctattataca gacaaaagat ccgtatcgca agagcaaata tacttttctg ctcaacattc
    18121 aatagtttac ctgaaagata aaaagaattc aagaaaaaca gtagatttct ctttgtttct
    18181 ttattatcct ttttccataa aataatttta ggtataaaag acgaatatta ttgggcaatc
    18241 cttctttttt ttgttatgat acatgtgaaa tctttcgtaa aggagaaaga aaatgcgcaa
    18301 agctcaaaga catttgctga ttaaacaagt cattgaagaa ttcccgatcc gtacgcaaga
    18361 agaactatta aacaaacttg ggcattatgg cgttaccgcc acgcaggcaa cgatttctcg
    18421 agatatccgt gatctaaaaa tcgtgaaggc acctgatgaa aatggcgttt cccgcttcgt
    18481 attgtttcgc gaaaaaggaa ctcctgagac taggactgag gaagaggatc ggctggttca
    18541 aatgattgaa gacattgtat tgaaagtcga acgagtccac tttttgacga tcgtcaatac
    18601 cttgccagac aatgctcatt tatttgcatc tgttttagat gagatcaaac ctccctctat
    18661 tgtcagtaca attgctggat tcgatacgat catggtcatc tcggaaaatg aggaggacgc
    18721 aaaacgagta gaagaatttc ttcaccatcc aactgaagta tcactttttt aatcagagca
    18781 cgtattccta atatggaatg cgtgtttttt tattttcaat attttcctta ttaaataaac
    18841 ctttacactc tttacaaaaa tacatcattt tttattcaat tttttcaaca ccttgattga
    18901 atcacaataa tcatcattta tattggatgt taacaagagc agcttcttgc tctttgtgta
    18961 tttcaaaaaa gaaatgaaag gaaatcatat gtataaatta ttaactcctg gaccattgac
    19021 gacttctgaa acagtgaaaa atgctatgct gatcgatcat tgtacttggg atcaagaata
    19081 caaagaaatt acacaatgga taagaaaaaa attattggat cttgctcaag tatcggaagg
    19141 aatatacaca acggtcttaa tgcaaggtag cggctccttt ggggtagaat cggtattaag
    19201 ttctgtgatc aggccagaag atactgtact gatttgttca aacggcgcat atggacaacg
    19261 aatgatcaac atgtgcgaat gtctgaaact gaactatacc gtttatgagg tatcggaaca
    19321 tcaaatcctc caagctcatg tgattacaca gctgattgaa gagaatccct ctatctccgc
    19381 actagcaatg gtccatagcg agactacttc cggtattttg aataatttgg aagagattgc
    19441 tgctgtcaca caagaaaaag atcttttgtt tatagtggac gcgatgtcaa gctttggtgg
    19501 gataccgatt cgtgtagccg aactagggat tgattttttg atttccagct caaacaaatg
    19561 tattcaaggt gttccaggct tttcttttgt acttgctaat aaagcggaac tagaaaagcg
    19621 aaaaaatgtg agtcgaagtt tgtcacttga tctatatgac cagtaccgcg tgatggaaaa
    19681 cgagaatggc aaatggcgtt tcacttcccc tacccatacg gttttggctt ttcaacaagc
    19741 gctgaaggaa ctagaggcag aaggaggaat cgaaactcga tattcacgtt atctcgccaa
    19801 taatcatgta ctacgacaaa aaatgacaga gatggggttc cagcctttca ttcaagaata
    19861 tcaggggcca ttcatcacct cttttcttta ccctaaggac tggcgatttg attttaatca
    19921 tttttatcag tatttaaagc aaaacggatt tgtgatctat cccgggaaaa tcagcgatca
    19981 tgaatgtttt cgtatcggaa atattggcga gatctatccg gaagatattt tggaactgac
    20041 agcaattatc aaagactata aggagaacgt taatgattaa aactgtaatt tttgattggg
    20101 caggaacaac tgtggacttt ggctgtatgg ctcctgtcca cgcttttaga aatgcttttc
    20161 tagaaaaagg aatccaactg actgacaaag aaatccgcga accgatggga aaattaaaat
    20221 gggatcatat ccaacagctt ctttccctcc cttctgtcaa aaaacaatgg atagctattt
    20281 atggacaact acctcaggaa aaagaggtgg aggaattgta tcaacaattt gaacattatc
    20341 tgtttaaaga tttggtcaag cattctacct tgaaacccga cacattggaa acgatcgaac
    20401 ggttgagaaa cgctgggatc aatattggtt ccacgactgg atttactgct cctatgatga
    20461 caatcgtagc agaaaccgca aaaaaagaag ggtattcacc agacttcatg gcaacttctg
    20521 aagacgtcga agggtatggt cgaccgtttc cttatatgat ccataaaaac atgcagtact
    20581 tcaaaaacaa gtcggttgat gaagtaatca aagtcggaga tacggtctca gatatccagg
    20641 aagggaaaaa tgccggagtg ttgacagtag gtgtaattga aggtagttcg ctaatgggga
    20701 tttcttatga tgagtaccaa aaattagaga tggggaaaaa agatcgttta aaacagtctg
    20761 ttgctcttag attccaacaa gctggcgctg atatttgtgt aaacaattta aaagagttaa
    20821 cagaatacat tttgtacgct gacaagaatc cagaatgact tttatacaaa atcagaggtc
    20881 gtgaaaaagc tgtactgcat caagtaataa gaacagccaa acaaaaatag ctcctcatat
    20941 ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    21001 aaaaaaggga gctgtgacac gacttttgtc acagtcccct tatttacaaa caaacagtgt
    21061 ttaagagaca acctctccac taaacggtgt tcattcagct gactctcggt atttgctcaa
    21121 aaaacgataa aatttgaaaa gctattttct cttttgtttc tctaatactc ggatcagaac
    21181 gctgatttca cattctcttt ttcttacatc ttatctggtg catgaagtcc tagcaaacga
    21241 aggtcttctt tcaataatac agtcactgca taaactagag ctaagcgtgc ttctttttgt
    21301 tcatcatcag ctaaaatctt cgtatgagca tagtatttgt tgaatgcttg tgctaatttg
    21361 attgcatgtt tcgcaatcac tgacggttcg tatttttccc cagcagacaa gaccgtttca
    21421 gggtattttt gaactaattt aaccacttcc cagctggtat cgtcattcaa tgcatagtct
    21481 gctgtttctg aagggacaaa tcctgctttt tccaacaggc tgactgcacg agcatgtgta
    21541 tattgcacat aaggtccagt ttctccttca aatcgaacaa cttcttccag gttaaagtca
    21601 aatgtattca gtcgatcgtt tttcaaatcg tggaaaatga ctgccccaac cccaacttgt
    21661 ttagctacag catctttgtt ttccaaatca gggtttttct cgctgatttg ttctttagca
    21721 gattggattg cttcatttaa tacttcttct aataagacga tctttccttt acgagttgat
    21781 aattttttac caccttgcgt gatcaaacca aatggaatat ggcgcatatc gtcagaccag
    21841 tcaaagccca tttccttcaa gacagctttc aactgtttaa aatgatagct ttgttcattc
    21901 cctactacat atagagattg tttgaagtcg taagtacgtt tacggtacaa tgcagcagct
    21961 aagtcacgtg taatgtatag tgtcgcaccg tctgacttct taatcaacgc tggattcagg
    22021 tcataagcag ataggtcaac gatctctgca cctttatcct cgttcagcaa atgtttttct
    22081 tctaaaagtt tgacgatttc atccatcttg tcattgtaga aagcttcacc gttcaacgaa
    22141 tcgaaacgta cttccagcag atcatagatt ttattgaatt ctttcatcga ttcatcacgg
    22201 aaccattgcc ataattgaat agcctcttca tcgccttctt ccagacgttt gaaccaagca
    22261 cgagcttctt catttaattc tggttctgtt tcagctactt catggaattg gacatataaa
    22321 cgaagcaatt cattgattgg ttctgcttta acagcttctt ctgtacccca ttttttgtaa
    22381 gcgacgatca gttttccaaa ctgtgttccc cagtcaccta aatggttgat gcggattggc
    22441 tgataaccga ttttttccat aatgaaaccg atcgaattac cgataactgt cgaacgcaaa
    22501 tgtcccattg agattggttt ggcaatatta ggagaagaca tgtcgattgg tactgttcct
    22561 tgattcccga tattgctatc cccataatgt tctttttctt tcactactgt ttgcaaaact
    22621 tttttgctga taagttcttt attcatgaaa aagttcaagt aaggtccgac aacttcgatt
    22681 ttttcaaagt tggcagaatc gattttttct gctaaatctg cagcgatttg ctgtggcgct
    22741 tttcggtaaa cttttgctaa tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat
    22801 tttggatttt ctagtaattg ttcgacttgt tccagtgtta aatcatcttt gacagcgtca
    22861 tagacagctt ttgcgacaag atctttatta ttcatcttta ttcctcctaa agtgctccct
    22921 tgattgttaa acaaaaaaaa tctctagcga cggcatactt agtatgccat cactagagac
    22981 gagatttccc gcggtaccac tccaattgtc ctaaaaaagg accagctcca tctgatatcg
    23041 gtagacgacc gtctgtttaa aacagaattc tccaagtgcg cttcgttgta tcttcttcat
    23101 tcggcttcca ccacttccga attcgctagc caagaatgat acaagtactc tcttgttcaa
    23161 caaatcttag tcttattaaa ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt
    23221 cgtttctgat aaaaaaacaa tccagatggc ctaaaggaga agaaatacca cctggatagc
    23281 tataaatcat ttcgtaaata cgaaagactt aacaaattaa tcagctacga taatcgttcc
    23341 ttcttcagaa gctaaaagat tctcgatatt ttctaaagaa gtgatgattg ccttagcgtt
    23401 tggttttgct tcaacaaatg caatagctgc ctctactttc ggaagcatgc ttcctggagc
    23461 aaattgcttt tcatcaatgt attgtttcat ttctgaaaca gtaacagttt ctaatttctt
    23521 ttgatctggt ttttggtaat tgacatacac attgtctact cctgtcaata cgatcagcaa
    23581 atccgcatcg atgatttcgg ctaatttttc tgaagcaaaa tctttatcga ttacggcttc
    23641 tcgtccttct aatccagtag ctgtttctac aacaggaatc ccaccgccac caacagagac
    23701 tgtgataact ccttgatcga ccagcgtttc gatgacacgt gcttctttaa tcgagatggg
    23761 tttaggggaa gcgactactt ttctccatcc acgtccagca tcttctttga atgtcacagt
    23821 ggagtcagca ttcatttgtt ctttggcttc ttcttccgtg tagaatggac cgactggttt
    23881 acttggattt ttgaatgaag gatcattttc atccacaata acttgagtga ctaaagaaac
    23941 cacatcttta tcgatccctt tttccttcag tacttcgccc atggcatttt gtaaccagta
    24001 accgatagat ccttctgtca ttgcaacaca tgtatctaaa ggcattgccg gtgttttctc
    24061 agaatcagca gcttgctgct gaatcaatag gttaccgact tgcggaccat ttccgtggga
    24121 gatgatcaat tcatctcctt gttcgataaa tttcactaaa tactttgctg tttccattaa
    24181 cgcttcttgt tgggcttttg cacttgcatc cgtagacaag attgcattac cccctaaagc
    24241 gacaactact tttcgtttgg ccatttcatt cgtcctttct tcagttctac cgaattttct
    24301 agataacaaa acgagccagc gttttgtgat tttccctcaa tgctgactcg ttttttcgtg
    24361 agatgaatgt ggttatgctt aaacgcgagg gataaacaag ttacctaatg ttgctgccat
    24421 gatcgctttg attgagtgca tacgattttc tgcttggtca aattgacgag catatttgct
    24481 gcggaagact tcatctgtta cttccatttc agtgatgcca aagcgttctt tcatttgttc
    24541 accgtaaaca gtgtttgtat catggaaagc tggcaagcag tgcaagaaga tcaaacgatc
    24601 tgtgttgtgt gttttttcta ccatttccat attgatttgg taaggtttca acaatttgat
    24661 acgttcttcg aatttatctt cttcacccat tgatacccat acatcagaat aaaggacgtc
    24721 tgctccgtct acacctttat caacatcgtc ggtaatcatc aattgtgcac cggatttttc
    24781 agcaaagcct tctgccattt tgacgatttc ttcttcaggt tgtaattctt ttggtgcaac
    24841 gatacgcata ttcgctccta aaattgcacc tgtgactaat aaagagttag ccatattgtt
    24901 tcgaccatct ccgcagtaag caacagtgat accttctact gttccaaagt tttcttggat
    24961 agttaaaaag tcagcgatca tttgtgttgg atgccattca tcagtcaagc cattccaaac
    25021 tggaacacca gagaattctg ctagttcctc taccattttt tgactgaatc cacggaattc
    25081 gattccatca aacatacgac ctagtacttt agctgtatct tctgtggatt cttttttacc
    25141 taattggata tcatttgcac ctagatattc aggatgtgca cctagatcga tcgctgcagt
    25201 agtaaatgca gaacgagtac gtgtagatgt tttttcaaat aaaagcgcaa tattttttcc
    25261 ttctagatag tgatgtggga taccacgttt tttcaagtct ttcaagtgtt ctgaaaaatc
    25321 gatcaaatat tgtaattctt cttttgtaaa gtctttttct gctaataagc ttcttccttg
    25381 gaatactgat tctttcatat taaacattct cctttagatt gtttttattt aagattaaag
    25441 aggaaatccc gacggcagct gacagtaaga ccgccgtcag gaaacaaatt acttcaagtc
    25501 ttcacggaca agtggttggc tcatgcagcg tggaccacca cggcctcgtg acaattcact
    25561 tgaattgatt tctaacactt tgatgccata gctacgaagc aattcgttcg atacataatt
    25621 tcggttataa gtgactacaa cacctggagc gattgccaat gtgtttgatc catcattcca
    25681 ttgttctcga ggtgcaacga tctcatctcc attaccagtt gggatcagta caagatcatc
    25741 taatccaaga gcagctttca tcgtttcgtg taagttatct gaatgagtaa tcttgatatc
    25801 atcaccatct ggttcgattg taaagacatc catcttaccg tctttacttt gaattgctgg
    25861 atggatagtg aatttatcgt gatctaccat tgtaaatacg gtatccaaat gcatcattgc
    25921 acggttgttt ggaattttga ttgctaacac tttttcaaat cctgaatttt ttgcgaatag
    25981 gtggcgtgct aatttttcta aagctttcgc attagttcgt tgagaaatcc caacagctac
    26041 tactttatcg cttaagatca attcgtctcc gccttcgata tgatctggat tttcacgatc
    26101 caaccatact tcgattcctt tatttgcaaa tcttggatga tgtttcaaaa tatattctgt
    26161 aaacatcgat tcacgacgac gtgcttcaaa agtcatctta ttgactgtca tcccatttcc
    26221 cattgatgcg gaagggtcgc gtgtaaagta caagtttggc attgggtcca tgtaaaatgg
    26281 atagtcatcg tctgctgaca gatcgtatag actgctagaa cgtacttcaa tatcttttgt
    26341 acgaacccca gccatgattt tatctaccat ttcttgcgtt tccattgata gaaggaactc
    26401 atgaagaccc tctcttacag cattcgatgc aatatgagat tcatccaaca ttttattcaa
    26461 gaattgttct ttgacattgc ctgcgtcaat cgcttctgcc gctaattttt ccaagtaaag
    26521 agtctcgacg ccttcatttt gcaatgtctt tgcaaagttg tcatgttctt cttgagcaat
    26581 cggaagatat ggaatatcat cgaacaacag acgatccata atatctggtg tcaaattttc
    26641 cacttcttgt ccaggccttt tcagtaaaac tgtctttaat ttccctattt cagagaaaac
    26701 gtgaataggt ttatccatgt tgtgttgcct ccttcttttt gattacatgt ataggatatc
    26761 ctccttttgt aatcactttc ataattttgt ctcattcaaa atgagacatt cctca
//
LOCUS       DAHHPA010000032        26528 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.033, whole genome shotgun sequence.
ACCESSION   DAHHPA010000032 DAHHPA010000000
VERSION     DAHHPA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.033"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(19..729)
                     /locus_tag="NBH55_001227"
     CDS             complement(19..729)
                     /locus_tag="NBH55_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCD1509223.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="NBH55_001228"
     CDS             complement(726..2198)
                     /locus_tag="NBH55_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCD1509224.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="NBH55_001229"
     CDS             2336..2809
                     /locus_tag="NBH55_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1509225.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="NBH55_001230"
     CDS             complement(2962..3513)
                     /locus_tag="NBH55_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCD1509226.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="NBH55_001231"
     CDS             3618..4148
                     /locus_tag="NBH55_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCD1509227.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="NBH55_001232"
     CDS             complement(4631..5074)
                     /locus_tag="NBH55_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1509228.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="NBH55_001233"
     CDS             complement(5090..6250)
                     /locus_tag="NBH55_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCD1509229.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="NBH55_001234"
     CDS             6757..8457
                     /locus_tag="NBH55_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509230.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="NBH55_001235"
     CDS             complement(8559..9392)
                     /locus_tag="NBH55_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCD1509231.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="NBH55_001236"
     CDS             complement(9696..11861)
                     /locus_tag="NBH55_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1509232.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="NBH55_001237"
     CDS             12121..14415
                     /locus_tag="NBH55_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCD1509233.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="NBH55_001238"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="NBH55_001238"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCD1509234.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="NBH55_001239"
     CDS             15066..16073
                     /locus_tag="NBH55_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCD1509235.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="NBH55_001240"
     CDS             16095..17114
                     /locus_tag="NBH55_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1509236.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="NBH55_001241"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="NBH55_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCD1509237.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /locus_tag="NBH55_001242"
     CDS             complement(18871..20292)
                     /locus_tag="NBH55_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1509238.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="NBH55_001243"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="NBH55_001243"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCD1509239.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /locus_tag="NBH55_001244"
     CDS             complement(22703..23959)
                     /locus_tag="NBH55_001244"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCD1509240.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="NBH55_001245"
     CDS             24539..25840
                     /locus_tag="NBH55_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCD1509241.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="NBH55_001246"
     CDS             complement(25909..26508)
                     /locus_tag="NBH55_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1509242.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         8047 a         5470 c         4757 g         8254 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 taaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatt
//
LOCUS       DAHHPA010000033        26247 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.034, whole genome shotgun sequence.
ACCESSION   DAHHPA010000033 DAHHPA010000000
VERSION     DAHHPA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.034"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            237..1877
                     /locus_tag="NBH55_001247"
     CDS             237..1877
                     /locus_tag="NBH55_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCD1509243.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="NBH55_001248"
     CDS             2031..3353
                     /locus_tag="NBH55_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCD1509244.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIATLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="NBH55_001249"
     CDS             complement(3415..3846)
                     /locus_tag="NBH55_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1509245.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="NBH55_001250"
     CDS             complement(3910..4746)
                     /locus_tag="NBH55_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCD1509246.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="NBH55_001251"
     CDS             complement(4936..5238)
                     /locus_tag="NBH55_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCD1509247.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="NBH55_001252"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="NBH55_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCD1509248.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="NBH55_001253"
     CDS             complement(5692..5958)
                     /locus_tag="NBH55_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCD1509249.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="NBH55_001254"
     CDS             complement(6212..6937)
                     /locus_tag="NBH55_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCD1509250.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="NBH55_001255"
     tRNA            complement(7303..7373)
                     /locus_tag="NBH55_001255"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="NBH55_001256"
     CDS             7490..8242
                     /locus_tag="NBH55_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD1509251.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="NBH55_001257"
     CDS             8258..8737
                     /locus_tag="NBH55_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD1509252.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="NBH55_001258"
     CDS             complement(8988..10568)
                     /locus_tag="NBH55_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCD1509253.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="NBH55_001259"
     CDS             complement(10561..11304)
                     /locus_tag="NBH55_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509254.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="NBH55_001260"
     CDS             complement(11480..12256)
                     /locus_tag="NBH55_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1509255.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="NBH55_001261"
     CDS             complement(12504..13340)
                     /locus_tag="NBH55_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCD1509256.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="NBH55_001262"
     CDS             complement(13351..14280)
                     /locus_tag="NBH55_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCD1509257.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="NBH55_001263"
     CDS             14472..14825
                     /locus_tag="NBH55_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1509258.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="NBH55_001264"
     CDS             complement(14899..15555)
                     /locus_tag="NBH55_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCD1509259.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="NBH55_001265"
     CDS             15781..17715
                     /locus_tag="NBH55_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD1509260.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="NBH55_001266"
     CDS             complement(17801..19024)
                     /locus_tag="NBH55_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCD1509261.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="NBH55_001267"
     CDS             complement(19018..20916)
                     /locus_tag="NBH55_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCD1509262.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="NBH55_001268"
     CDS             complement(20910..22028)
                     /locus_tag="NBH55_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCD1509263.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="NBH55_001269"
     CDS             complement(22111..22944)
                     /locus_tag="NBH55_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD1509264.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="NBH55_001270"
     CDS             complement(22937..23854)
                     /locus_tag="NBH55_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1509265.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="NBH55_001271"
     CDS             complement(24081..25205)
                     /locus_tag="NBH55_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD1509266.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..>26247
                     /locus_tag="NBH55_001272"
     CDS             25388..>26247
                     /locus_tag="NBH55_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1509267.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAF"
BASE COUNT         8147 a         5206 c         4416 g         8478 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaacccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaa
//
LOCUS       DAHHPA010000034        25899 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.035, whole genome shotgun sequence.
ACCESSION   DAHHPA010000034 DAHHPA010000000
VERSION     DAHHPA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.035"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(40..240)
                     /locus_tag="NBH55_001273"
     CDS             complement(40..240)
                     /locus_tag="NBH55_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1509268.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(545..1777)
                     /locus_tag="NBH55_001274"
     CDS             complement(545..1777)
                     /locus_tag="NBH55_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCD1509269.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2031..2600
                     /locus_tag="NBH55_001275"
     CDS             2031..2600
                     /locus_tag="NBH55_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1509270.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLC
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2778..3218)
                     /locus_tag="NBH55_001276"
     CDS             complement(2778..3218)
                     /locus_tag="NBH55_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCD1509271.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3376..4140
                     /gene="map"
                     /locus_tag="NBH55_001277"
     CDS             3376..4140
                     /gene="map"
                     /locus_tag="NBH55_001277"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCD1509272.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4172..5095
                     /locus_tag="NBH55_001278"
     CDS             4172..5095
                     /locus_tag="NBH55_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCD1509273.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5171..6310
                     /locus_tag="NBH55_001279"
     CDS             5171..6310
                     /locus_tag="NBH55_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCD1509274.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6303..7103
                     /locus_tag="NBH55_001280"
     CDS             6303..7103
                     /locus_tag="NBH55_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1509275.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7103..7930
                     /locus_tag="NBH55_001281"
     CDS             7103..7930
                     /locus_tag="NBH55_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1509276.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7908..8642
                     /locus_tag="NBH55_001282"
     CDS             7908..8642
                     /locus_tag="NBH55_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1509277.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8742..9608
                     /gene="rfbA"
                     /locus_tag="NBH55_001283"
     CDS             8742..9608
                     /gene="rfbA"
                     /locus_tag="NBH55_001283"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCD1509278.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9622..10194
                     /gene="rfbC"
                     /locus_tag="NBH55_001284"
     CDS             9622..10194
                     /gene="rfbC"
                     /locus_tag="NBH55_001284"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCD1509279.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10216..11244
                     /gene="rfbB"
                     /locus_tag="NBH55_001285"
     CDS             10216..11244
                     /gene="rfbB"
                     /locus_tag="NBH55_001285"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCD1509280.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11342..12193
                     /gene="rfbD"
                     /locus_tag="NBH55_001286"
     CDS             11342..12193
                     /gene="rfbD"
                     /locus_tag="NBH55_001286"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCD1509281.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12228..14261
                     /locus_tag="NBH55_001287"
     CDS             12228..14261
                     /locus_tag="NBH55_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509282.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14305..15585
                     /locus_tag="NBH55_001288"
     CDS             14305..15585
                     /locus_tag="NBH55_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCD1509283.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15795..16601
                     /locus_tag="NBH55_001289"
     CDS             15795..16601
                     /locus_tag="NBH55_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509284.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16613..17833
                     /locus_tag="NBH55_001290"
     CDS             16613..17833
                     /locus_tag="NBH55_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509285.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17823..19409
                     /locus_tag="NBH55_001291"
     CDS             17823..19409
                     /locus_tag="NBH55_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCD1509286.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19448..21586
                     /locus_tag="NBH55_001292"
     CDS             19448..21586
                     /locus_tag="NBH55_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1509287.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAESTHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(21954..22937)
                     /locus_tag="NBH55_001293"
     CDS             complement(21954..22937)
                     /locus_tag="NBH55_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCD1509288.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23315..24706
                     /locus_tag="NBH55_001294"
     CDS             23315..24706
                     /locus_tag="NBH55_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCD1509289.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24722..25762
                     /locus_tag="NBH55_001295"
     CDS             24722..25762
                     /locus_tag="NBH55_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1509290.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25781..>25899
                     /locus_tag="NBH55_001296"
     CDS             25781..>25899
                     /locus_tag="NBH55_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCD1509291.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIY"
BASE COUNT         8213 a         4384 c         5277 g         8025 t
ORIGIN      
        1 acgtacaaat aacccctgta tcctttgcgg tacagggggt tattttgtat aagtagacat
       61 tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg
      121 ataaagtaaa atagatgcat gcgtatgtct tagtccatca ataaacagtc gaagagaaaa
      181 tagggaaaaa ttgattacta aagaaaaact tactaggtcg acggtgtagt tgattttcat
      241 acttcaatag attcgaatta aacgatttcg attggcttat accagtccta tccaacgaaa
      301 gatttatcgt tcttaactct agcggtataa cttgaagctt tacgtaaagc caatgggaat
      361 gaactggaga tgacttttta ttatttttag taatagctga tgggttaata cttacaactt
      421 tccttttcaa agtaccatat aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa
      481 gccaatagct tagatccttt attctatgct ttatattatt ttttgcatgt gtgcgaaagt
      541 atactcaagc ccaatcgcca tttcggaaaa tcggtactgt agagccatct tcacggattc
      601 cgtcgatatc catcttgtca gatcctacca tgaaatctac gtgggtttga cttctattca
      661 atccagcttc tgctaattct tcatcggaca tttctgtacc gcctttcaca ctgaaggcat
      721 aagcagaacc tagtgctaag tgattcgatg cgttttcatc aaataatgta ttgaaaaaga
      781 tgatgccaga ttgagaaata ggtgaagggt caggtacaag tgcgacttct cctaaacgac
      841 gcgcaccttc atctgtatca agcagttttg ctaagacatc ttctccttgt tcggcagaaa
      901 aatcaacaac ttttccatct ttaaaagtaa atttcatacc cgagatgatg gtgccagcat
      961 aactcagtgg ctttgtactt gaaatgtaac catctactcg atgactgtca ggtgctgtga
     1021 acacttcttc cgtcggcata tttgccatga atttttctcc acgagcatta tagcttccag
     1081 cgccttccca aagatggttt ttaggcaatc cgataatgat atctgtaccg ggagcagtat
     1141 agtgaagggc agaaaattgt tctcgattca attcttctgc tttttttgct aatttttcat
     1201 catgcttctt ccatgctaag acagggtctt cttcatagac acgagtcgtt ttgaaaatct
     1261 gatcccataa tgcctcaact tgtttttctt ctggcagttc tgggaagact tttgctgccc
     1321 attgtttacc agccgcagcc acgacagtcc aactgacttt atttgcttga gttgccttgc
     1381 gtaaattcat tagtgctttg ccattcgctg attgataaga cgctactcga tggctatcta
     1441 caccagcaaa tgcatcggga tcggcagaaa caacgctgat cctgcttgct cctttttcaa
     1501 gccattcatc cgcttgatcg attttagatt gtggtacatt ctcaatacga tccgttgcag
     1561 catgtaaaag aaattctctt tggatctggt cgtcagtcca ttgaacgata acctcagcgg
     1621 ctcctagttt ataagcttct tgtgtgatca atcgagcaag gggtgcctgt tccacgctga
     1681 tctgtaaaac aactgtgtgt cctttttctg tggctacgcc agtttctgca attaagcgtg
     1741 catatttttt tagaagttca ttaaaatcag ataacataat aaaaattcct cctcaccatt
     1801 ttttctggta actattagca tgataccatt taattatgat tcaaacaatg aggctttaaa
     1861 taaaaaaagg aggttatttt tttaaggtta agtttcatga tatatgttac tacttgctat
     1921 ttcagcttgt gaagaaaatc aaaaactaat tttgcatatc aaaaaaacat gttacaatga
     1981 aattaatcaa tggtggaagc gttataaaaa aattaaggag attaaaaaat atggcaagaa
     2041 aaaagacgat tactagagaa cagattttgg cagctgctta tgaagtagct gccaccgatg
     2101 gatttacacg atttactgcg agaaacatcg cgaacaaaat gaattgttcc acgcaaccga
     2161 tttatcttga gtttaaaaat atggatgact tgagacaagc tctttttgag cagatctatg
     2221 atcatttaca atatgaagtg ttcactgttg agcatacagg gaatacgatt atcgatcttg
     2281 cttttaatta tatccatttt gcaagaagag agaagaagtt gtatcgttca ctgtgcttag
     2341 aagaatatgg tggaggaaaa gagatgcaga atttctctta tacttacttt gtgaatacag
     2401 tgaaacgcga tccagcttat gtgaatcttt cagaagagca gatcgaatct ttgtataatg
     2461 ggacatggat catagctact gggatcgcag ctttgatgtc ttcaagcatc atccatccaa
     2521 ctgatgaaca gattgaaaag atgatccaag acagtataga tgctatcttg aaattcaaag
     2581 aaccaatcga aatcgattga atatactcaa aagttccaca aatcagtagg taacaagaga
     2641 tgtgtcaaaa taaggaaaag ttcgtttttc ttacagcgta tgcattttta actcccgaag
     2701 aactcttcca tgaatgcaat gaataaaaga gccatacaga ttgaataaat ctggatggct
     2761 cttttattgc aagtatatta aatttttgcg actagttctt tagcgaaagc ttctaaattt
     2821 tcgatatctt cttcttcagc attcagatcc actttaacat tttcggctcc ttttgatgcg
     2881 cctgtttttg caaaagcagc atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa
     2941 gtatcaccag atccgcagac accgtaaatt tttcccaata gatccaattc ttgcaagtct
     3001 tcataaaagt ctacgatctc atcaggcaga tcgccatcat cgtatgtata agtagctact
     3061 acgcaaatat ctgcctcctc gaattcatct gcatctactt gggtacattc gttgatttcg
     3121 acttcgatat ccagattttc aaatgcttct gcaacaatat cggcgatttc ttcagtgttt
     3181 cctgtcatgc tagcatatac gatttttgct aaagccatga tgtgcctcct cgtttataat
     3241 tatttaacaa ttatgaatta taccaaaaca tcattgaaaa gaaaataaaa cctaaagaca
     3301 cctggggaat gtcatttggc taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa
     3361 aaaggagacg aaaatatgat tactttaaaa tcagcacgtg aaattgaaat gatggcagaa
     3421 tctggagctt tattagcaga tgtacataaa aatctgcgtg attttataaa accaggaatc
     3481 acaagttggg atattgaggt atttgtcaga aactatatcg agagtcatgg cggaattgct
     3541 gcgcaaattg gctttgaagg gtatgaatat gcgacttgta tcagtataaa tgatgaaatt
     3601 tgtcatggat tccctcgtaa aaagcctttg aaaaacggtg atttggtaaa agtcgatatg
     3661 tgtatcgatt tgaaaggcgg catttctgat tcttgttggg cctatgtagt tggagaatct
     3721 acacctgaga tcgaccgatt gatggaagta acgaagaaag ctttgtacat cggtattgag
     3781 caagcacaag tagggaatcg gattggtgac atcggtcatg ctatccaaac ttatgtagaa
     3841 ggcgaaaatc tagcgatcgt tcgtgatttt attggtcatg gcgttggtcc tacgattcat
     3901 gaagaaccag cagttcctca ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc
     3961 atggttatca cgatcgaacc tatggttaat accggaactt ggaaaatgaa aatggatcct
     4021 aacggctgga ctgcgtatac aagagacggc ggactttctt gccaatatga acatacattg
     4081 gctattacta aagatggtcc aagaattttg acatcacaag gagaagaagg cacttattga
     4141 tctatccagt caataggtac gggagaggta catgaaagtt tggaacaaaa taaaacaaaa
     4201 tgaaaatctg atgcgcttta ttgagacgac tcaaaagcgc atcactgatt cagaaatgag
     4261 tacgacctct gtcgtcgttg cttattattt actcttgtct ttatttccat tgattattgc
     4321 ttttggtaat attttaccgt ttctccacat cgatcaagag actgtactga cctatttacg
     4381 agaagtgatt cctgagaccg tttaccagtt tatcggtcct gcaatcaaag accttttgac
     4441 acaaagttca ggaggcttgt tatccgtttc tgcattagcg gctttatggt ctgcaagtca
     4501 aagtatcaat gctttgcaaa ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa
     4561 ttttttgatc gtccgtttct tctcactaat tgtcattgtc ttgttcatga ttgctattag
     4621 tggggtaact atcgttcttg ggttgggaca gctgattttg gatgctattc agccaatcgt
     4681 acaaataccc gtgaacttta tcgatcagtt ccaaactttg aagtggccaa tcacattggt
     4741 agctttattt gtcatcatgt tcttgattta tttaattgtc cctaatgctc agttgaagtt
     4801 gaagacgatc attccgggca cgatcttcgc tactgtcggc tggatgctgt tatcacaagt
     4861 gtttagtatc tatgctaggt attttgcaac gaggatcagc ggttatcaga tcataggaag
     4921 ttttattgta ctcatgcttt ggcttaattt tgctgctacg atcatcattt tgggcggaat
     4981 catcaatgca gttgttcaag aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc
     5041 aagccgaaaa tggttcaata aattgaaaac taaattttct cataaaaaaa agtagaaaca
     5101 agagttctta ttgtaaattt atatcagtaa tttataataa ttatgactct taattaaagt
     5161 aggtgcccat atgcttgttt cttttagtat ggttataaga ttttttctga caattttatt
     5221 atcactgata ttaacacctg tattcaaaat catttcagta caaacaggta tggtcgacaa
     5281 accaaatgaa agaagaataa ataaagttcc aatgccttca gcgggcggac tgcctatctt
     5341 tatttcattt gttatatcga ctttattctt attccgtaaa attatccctc aatcgtatat
     5401 tgtaccgatc ttgattgctt ctttagtaat catagtcaca ggagtgctcg atgataaata
     5461 tgagctgaca cctaaacaaa aaagtatagg gatcttgatt gcttctctta ttatttattt
     5521 tgtagcagat atacgtattg attccttcac gcttcctttt attggatata tccaactagg
     5581 ctggcttagt tttcccgtaa cgattttttg gatcttcggt atcacgaatg cggtcaatct
     5641 gattgacggt ttggatgggc tggcggcagg gatttcattg atcggtttaa ttacgatagg
     5701 aatcattgga tattttttcc tccatgcttc tacagtgtat attccagttg ttattttctg
     5761 tcttgtagca agtattatcg gtttttttcc ttacaatttt tatccggcaa agatttattt
     5821 aggcgataca ggagctctat tcctagggtt tatgatggcc gtcctttcac tacaagggct
     5881 gaagaacgtg acttttgttt catcgatttc tttgctgatc gtcatgggag tgccggtaac
     5941 agacacgttt tttgctatca ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga
     6001 taaaaaacat cttcatcacc gactaatcag tttaggcttt actcataaag gtgctgtcct
     6061 gacgatttat gcgatggctt tgatgttttc tttcacagct atggtcatga attatactgg
     6121 aagaatcgga acgatcatat tgattattgc catgttgttt gctgcaattt tattatttga
     6181 attgattggt ctgatcaatg agaaacatca gtttatttta aaaacattgc gatttttagg
     6241 gaataaagag taccgtacgc aagtaaaaca gaaatatttg aagaaatgga accgtcatat
     6301 agatgaataa caaattaaac cagccgatcc ttattagtat tgtgacggca aacagtgata
     6361 aaatttttcg gacactagat aattttattt caactgtaaa agatgaaaca gcagtaaaaa
     6421 tccgaatttt tgacaacaat tcacttcccg agtacaaaaa aagactaaca gagtatagga
     6481 aatatcccta catcgatatc tattttcatg atgaaaattc cggatttggt tatggacata
     6541 atcacaatct gatagctagc gattctcgct atggcattat ttgtaaccca gatattttag
     6601 tggatgaaga gacagttaca gcattggtct tgttattgaa agaacatccg gaatgtgcga
     6661 tgttagcacc taagattctt aatgaagatg gaacaactca gcacctcatc cgtgagcagc
     6721 tggctgtttt tgattacgtt ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg
     6781 acaaacgact ggctgtgttt gaatgccgag gattgcctac tgatgctaat agttatgtac
     6841 ggatgatctc aggtagtttt atggtagctg atattgataa atttaaagag ataaaggggt
     6901 ttgatgagcg tttcttcatg tactttgaag acaacgactt gtgtttgagt tttgaaaaag
     6961 caggttacaa gttattgtac acaccctttt atgcagttat ccatttttac gggaagggtg
     7021 cgcatcgaaa tgcaaaactg tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata
     7081 agtgggggtg gcgttttttc tgatgaaacc agaaatatta gtagtcgttg tgctttatca
     7141 gcaactattc agtcaatctc cagcttatgg accattaagc aaagcactta gcgaaaagca
     7201 aattcagctt attatttacg ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa
     7261 agaaaatatc gtttatcatc atgatccgca aaatcctggt ttagcggtgg cttataattt
     7321 tgcgttgtct caagcagatg aaggaacacg ttgtctcgtc actctggatc aggatacgag
     7381 gctagtcgac gattattttg aactattgcg taaagtaacc tttacagatg aatgtgttgc
     7441 tgcagttccg atgatttttt ctgggagccg acaagtttct cccctatatt cagatcgtta
     7501 tatcaatcgt acagctcaag cagtagaagt tgaaggccca acagctgaac gaatcatggc
     7561 aatcaattca ggtgttgcct ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc
     7621 tgctttttct ttagattttt tggatcattg gctgttttgg aaagtggatc agttggaaaa
     7681 aactgtggag atccttcctg tacgaatgga acatgatctt tcggttttag actatgaaaa
     7741 agtttcaagt aatcgatacc gttctatttt acaagcagaa aatcgatttt atcatgaata
     7801 tgatcaaagg catttatcca gtcaccgacg acaattattg ttaagaacag ctaaacagtt
     7861 cttgaccgtc aaaaaccggc atatctggcg gatgactttg cgctcatatg ttaagaattg
     7921 gaaggtgtga attcgtgcgt tcggtttgta tagctacctt taatggaggc agatatttaa
     7981 gagagcagtt agatagtatt ttatctcagc tttcagcaga agatgaagta gtcatttctg
     8041 atgatgggtc agtagatgat acatgggata tattaactga atacgcagaa aaagataagc
     8101 gaatccagct tttttcgggg cctcgtcaag gattgatcgc taattttggt tatgcgatcc
     8161 agcagacgaa aggagatcta atctttttgg cagaccaaga tgatgtttgg ttggagacaa
     8221 aagtgaaaaa gatcactgac tattttgagc agaatccgga tcagctagta gtagtttctg
     8281 atttaatcat tgtggacagc gagctgcaag agatccatga ttcgtatttt tcgtttcgaa
     8341 attccaaaga aggatggctt aataatgtga taagaagtca ctatattggt gctggtatgg
     8401 ctttccggtc ttctttaaaa cccgatattt tgccgatccc gtcagaagtg ccgatgcatg
     8461 atatgtggat tggtttgctg ggaggaaaga agactgggtt cctaagagaa ccgttaactc
     8521 tgtaccgaag acatgattta aacgcgagtg agattgaaac acattctaaa ttacggcaaa
     8581 aaatttcttg gcgtgttcat ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt
     8641 gataaaagat gtttaaatcc ttaatatttc catgacaaat ataacaattg atgagataat
     8701 aacaaagtga aatttgaaat caaaaaggag agcagatatc catgaaggga atcattcttg
     8761 ctgggggcag cggaacacgt ctgtaccctt taacaaaggc aacatcgaaa caattgatgc
     8821 cgatttatga caaaccaatg atttattatc caatgtctac tctgatgttg gcgggaatca
     8881 aagaaattct gattatttca acgccacaag atacaccacg tttcaaggaa ttatttggaa
     8941 acggaaatga tttaggactg cagatcgaat atgcagtaca agaaagtcca gatggattgg
     9001 cacaagcgtt tatcattgga gaagaattta ttggagatga tagtgtctgc ttggtattag
     9061 gggacaatat ttactatggt ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag
     9121 atacaggcgc gacagtattt ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag
     9181 aatttgatga gaatatgcaa gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa
     9241 attacgctgt aactggcctt tatttttatg acaatgaggt aatttctatc gctaaaggta
     9301 tcaaaccttc agaacgtgga gaactagaga ttacggatgt caataaagca tatttggata
     9361 aaggaaaact atccgtagaa cttatgggac gcggatttgc ttggcttgat acaggaacac
     9421 atgaatcatt gcttgaagca tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa
     9481 aagtggcttg tttggaagaa attgcttacc gtatgggcta catcgataag gaacaattgc
     9541 tggcccttgc acagccattg aaaaaaaatc aatacggtca atatttattg aatttggccg
     9601 ctgaataagg agtccttaca gatgaaagta acagatacaa aattaacaga tgtcaaaatc
     9661 gtagaaatgg acgtctttgg cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca
     9721 aaatttgcag aacatggatt gaaatatgac tttgttcaag acaaccattc actatcagct
     9781 gaagctggtg tccttcgggg gcttcatttc caaaaaggag aagctgcaca gacaaaattg
     9841 attcgggtag tcactggagc agttttagat gtagtagtgg atatccgcaa aggtagcccg
     9901 acttatggtc aatgggacgg atttatctta tctgaacata atcatcgaca gttgcttgta
     9961 cctaaaggat atgcccatgg attcgttact ttgacaccaa atgtgaactt cctttataag
    10021 tgtgataatt actataatgc agaagcagat gggggcatcg catttgatga tccagattta
    10081 gcgattgatt ggccaattga tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat
    10141 ccgacattaa aagaatttga agcagaaaat ccatttgttt acggagaaat ctaagaatta
    10201 aggagaaatt ttcttatgaa aaacatcatt gttaccggag gtgccggatt catcggctct
    10261 aatttcgttc attacgtggt aaacaatcat ccagaagtgc atgtcactgt tttggataaa
    10321 ctgacttatg caggaaacaa agagaacttg gccggacttc ctagcgatcg cgtagaattg
    10381 gtcgtaggag atgttgcaga tgcagaatta gtggaccgtc tagtaaaaga agcggacgca
    10441 gttgttcatt acgcggcaga atcccataac gataattcat taaaagatcc tttcccattt
    10501 gtacaaacga atatcattgg tacgtataca cttttagaag cttgccgcaa gtatgatgta
    10561 cgctatcatc acgtatctac tgacgaggtt tacggcgatc ttccattacg tgaagacctg
    10621 ccaggacatg gggaaggcga aggagaaaaa ttcactgcgg aaacaccata taatccttca
    10681 agtccatatt cttctacaaa agctggttca gatctgttag taaaagcatg ggtgcgttct
    10741 tttggcttac gtgcaaccat ttcaaactgt tcaaacaact atggtcctta tcagcatatt
    10801 gaaaaattta ttcctcgcca agtaacgagt atcttaagcg gtattcgtcc aaaattatat
    10861 ggcgaaggaa agaatgttcg tgactggatc catacgaatg atcattcttc agcagtgtgg
    10921 ctgattttga cgaaaggcag aattggcgaa acctatctga tcggtgcaga cggtgaagaa
    10981 gataataaga ccgtgatgga attgatctta gaaatgatgg gccaaccaaa agacgcatac
    11041 gaccatgtaa acgaccgcgc agggcacgat ttgcgttatg caatcgattc gacaaaattg
    11101 cgagaagagc taggttggca gcctgagttt acgaacttcc gtgatgggtt ggctgacacg
    11161 atcaaatggt acgaagaaca tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca
    11221 gcatatgcca aaaatggaca ataatacaga ggaaatttca ctgtattaat ggaaaaacac
    11281 atgtgaggca ggcgctcgcc tgtctctttt ttaaaataaa aatcaaggag cggataacca
    11341 tatgttttta ttaactggag gaaatggaca attaggtaca gaattgcgcc atttattaga
    11401 tgaaaaaaat ctgccatatg tttcaacaga tgcaaaagac ttggatatca cagatgctga
    11461 gaaaacaatg gcttatatta cagaattaaa acccgatgtt attttccatt gcgcagctta
    11521 cacagcagta gataaagcag aagaagaagc aaaagaatta gatgagaaaa tcaatgtcga
    11581 tggcacaaga aatgtagcac ttgcagcaaa agctgtgaat gcacaattcg tctatatcag
    11641 tacagactat gttttcgatg gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac
    11701 gaatccacta aatgaatatg gtcgtacgaa attattaggc gaaaaagcag tagaagagat
    11761 tttagaagac tattatatca tacgaacttc ttgggttttc gggatctatg gacacaattt
    11821 cgtcttcacg atgcaacgcc tagcggaaac ccgcgacaaa ctgactgtag taaatgacca
    11881 gtttgggcga ccaacttgga cacgtacttt ggcagaattt atggtatatg tcatcgaaga
    11941 aaaagcacca tttggtattt atcatttgtc gaacgaaaat agctgttctt ggtatgagtt
    12001 cgcaaaagaa atattgaaag atacagatgt agaagttgca cctgttacgt cagaagaatt
    12061 tccgcaaaaa gcaacacgtc cgcagtattc agtcatgagc ctgaaaaaga cagaagcgtt
    12121 aggttttgtc ataccaactt ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa
    12181 aatgaataag taatttcctt tttatcaaat agaatttgga gcatgatatg gaaaaacaaa
    12241 caagaagtgc acgacgaaaa gaaaagccaa aagcgaatca ggcagattcg aatttggtgc
    12301 ttgacctgtt tctaaaactc cgctatctta ttggcgtttt agtcatcgtg gtcatcgtta
    12361 cttttaattt aaacggaagt tcacttggtg tttgggataa atatgtgtcg caaagagacg
    12421 atgggaaaaa aactgatgtg atttttggac agaatcggga agtgcggtca gacgaatggc
    12481 tggtgcagac acctttttac ctctcacaag ctgaagagga cttttcgttg gtgaacgagg
    12541 attattcatt aaacggacaa aacatgatca ttgcgtataa ttctcctgta aaggatatta
    12601 cggttatcgg aaaaccattc aactggggat tctttttcct cggaagagat cgaggacttt
    12661 ccttttattg ggcgatgaaa ttagtcgtca tggtcttgct tggattcgaa gtactgatga
    12721 tattaacgaa gaggaaaaaa gctctatcgc taatcggtgc atttgcgctg acattttctc
    12781 cagcagtcca gtggtggttc atgcaacatg tgggcgattt gattttcttt actttgggac
    12841 tgatgattgc tttttatcat tatttctaca atcatgataa aaaatggcta cgaacgttga
    12901 tgatgctttt ggtggttatt ttcggtattg ggtttatttt agtgatttac ccagcccatc
    12961 aagtgatgct ggcgtacttg ctgatttttt attttgttgg cctgcttatt tactatagca
    13021 agaaagtttc atgggatctg ttggatgtag tgttgattgg aggggcagtt ttatttattg
    13081 gcggagtcat ggttcatttc tggctaacgt caaaagacgc attgttagct tctttgaata
    13141 ccttatatcc aggaaaacgg gtaagtactg gaggaaagtg gacgattggc gaattttttt
    13201 ctttccttac aaattggaaa attccttttg aagatatcaa ctattcgaat aactctgaag
    13261 tggccctgtt ctatcacttt tttccaactg tattcctagc aagtccattt gtgttatttg
    13321 gcaaaaaaga tagcgaacaa aagttactgg gacgtatttt gatgattttt tgtctgtttt
    13381 ctatcttttg gattaccgtt gggttgccaa aatggctagc agaaatcact ctgctgtctt
    13441 atgttcctcc attaagagcc atccttactt ttagttttgc ggcttgttta ttatcgatct
    13501 ggtttatcgc ttatatttgg agagaagatg ttattcctat ttggtataaa ttcattttgt
    13561 tggcagtaaa tgcatggtct tatttctatg cgattacgca ttcgaaaatg gataattatt
    13621 tttcggattt tgaaaccatc gcagttgctg tgttgggaac agtgatttta gcttttgcat
    13681 tatttggctg gagacgcttg ttttacctcg cttttgtcgc cttagttatc gtatctggct
    13741 ttttcgtcaa tccagtcgta gaaggtacag gagcgatttt tgaaaaaaca ctggcaaaag
    13801 aaatccaaaa aatcgatcaa caagatcctg gtaaagtttg gttgtctgaa gatgaattat
    13861 ataacttcac cccaacattg ggagtgaaat ccttcaatac agtacgcttt tatccagata
    13921 tagctgcatg gaagaaaatc gatccgaaag aagaatacga gaagtattat aatcggtatg
    13981 ctcatacaag agcttttatc gctaaggaag atacaaagtt cattttagat cgtccagata
    14041 tgttttcggt cacgttgaat tttgaagacg cagaaaaatt agggatcgac tatgtgatca
    14101 gtaaacgacc attaactgat tacaaccaat tatatgaagc gcagttcacg cagctatatg
    14161 gaccggataa agacggttat atgattttta aagtagaata tccggattat ccagatgtac
    14221 cacaaacaca aactcaggaa aatcagctag tcgtacagta ataggctagc tgagaataat
    14281 cctgaaatat tagtagaaag gagagtgaag ataattcaag caatcgtaca aaaatttgtc
    14341 aacaaaatga gtgtcggttt cttgcttttg ttgctgacgc tagtcttttt tgtttggaca
    14401 gcaggggtag gttttccagc aactttgttt atatataaca atagttcatt acttatcgct
    14461 tttagtgtgg ctattttgct cattttaaat gtttataaag caaaaggcgt cgattttctt
    14521 tatctaggtg ttgcgcttgc ccttttcatt ttcttttctt actttcggga attaagaaat
    14581 gagtctttat ttggagactt actgtttcct ttgattattt tattttgttt agtaattaaa
    14641 tggatcgagt ggacaaaatg ggatcgcggt atttatctga ttgtctttac ggtcttcctc
    14701 ggaatcacgt tatatcgtgt atttacagag atccaagtac cagaaggaca aagcatctgg
    14761 gctccaggca attcgctttc tgatatttgg atcaatacga atacaatcgg aagttctttg
    14821 atgactcttg ctttattgat cagtggattt gccagttctt ttgaacgatg gtatgttcga
    14881 atcatcggtg ggatagcagt cgttgcagcc tttttaggta tttgggtctg ccaatctaga
    14941 ggtgcattag tcgcgttgat tgtttttgcg atattggatc ttttacctaa atcgttgatg
    15001 cgtgtcattc gtgcgccatt tattgcctac acggttacga ttcttttagc tttgccgatt
    15061 tcttatctgg cggctgtctc tgaaaaagtc aatttgttta ccgatcgaga agatatttgg
    15121 cataaattct atcagacgct aggagaaaaa tctgaacaag ttttactagg aatgaaaaca
    15181 tttatcttcc agcgaggaaa ccaattttta ggaaaccaca atagttataa ttctattttg
    15241 aatatctatg gattgatagg atttgggatt gctgctttgc tgcttatcct ttttattggt
    15301 cgcttaacgt tgaaagcaga tttatccaac gggcagatga cttttatctg ggcctttttt
    15361 gcggtgatga tgcagtcatt tatggaggat acattgactt catacccatg ggttccgatt
    15421 atttatatcc tgttggctat ggccgttcat cgatatgatg aaccagaagt tgcggaggaa
    15481 ttctatgtta tggaagaggc atatgacgat atgacttatg atgatacatc ttatgaggaa
    15541 caaccagata gcgctcattc ttccaggatc caacgctatc attgataggc acaaaatacg
    15601 agagaggctg tgacaaccgt cgcagccttt tttggtttat tgcaattgtc tagcatcttc
    15661 tgtaaaatag aaacgttaga gaaaatcttg tatcatagaa aattaagaaa gaaataagaa
    15721 cgcagctcgg taccaaaaat atggtaaaat ggcacttgac tgtaattcac tgaaaatcgc
    15781 ttggaggaac acaaatgttt aaagatttgt atttatttat gaaggacatt tatcagaaca
    15841 gaaaattatt gctacagttt tcgatcaatg acttcaaatc aagatatgca ggatcgtttt
    15901 taggaatctt atgggcgttt atcaatccgc tatttactgt attgatttat tggctggtat
    15961 ttggtgttgg actgaaagca caaatgatcg atgcaaaata cccatttatc gtatatttag
    16021 ttaccgggat gattccctgg ttcttcttct cagatgcatt tgcatcaact acggttgttt
    16081 ttcgggaata tacttattta gtaaagaaag tcgtattcaa tattcgtatc ttgcccacga
    16141 cgaaaatcct atccaatctg tacacccatt tattcttcat tctgattggc atagctgtcg
    16201 cgcttataaa tggtatatat ccttcagcaa tgtctttaca gctgatttat tatttatttt
    16261 gcatgtgtgc tttcttgaca ggattgactt ggctgacggc atcgatccaa ccatttttac
    16321 ctgatatcat gcaattaatt acgatcgtgc tgcagttgat catgtggaca ttaccgatat
    16381 tatggagccc taatcagttt gattcaggtc ttgtaagcat attgaaatta aatccgctgt
    16441 actatgttgt tcaaggttat cgtgagtcat ttttaagtga agcttggttc tgggagcatg
    16501 gaacatatac tttgtatttc tgggttttca cgttagtaat gttcgtaatc ggctctgcag
    16561 tgttccgtag attgaaacca catttttcag atgtgctgta aggagaatag ctatgacaga
    16621 atatgcaatt agactaaaag atatcacaaa atcttataat atgtacgcaa aaccttcgga
    16681 tcgatttcgt gaagccctca atccttttaa gaaatcctat catgatgtat tttatgcgtt
    16741 gaaaaacgtg aatgtagaaa taaaaaaagg tgagatgatc ggtttcgtgg gagaaaacgg
    16801 atcgggaaaa tctacaatgt tgaaaatcat tacgggagta ctgacaccaa catccggcac
    16861 gatggaaatc gaaggaaaaa tctcagcttt gctagaacta ggatcaggat tcaatcctga
    16921 gtattctggc tatgaaaata tctatttaaa tggaatggtt ctaggatttt caagagaaga
    16981 agtcgatgaa atggtcgatg atatcattga atttgctgac atcggagacc atatttatca
    17041 acctgtaaaa acttattcaa gcgggatgtt cgttcgtcta gcttttgctg tagcaatcaa
    17101 tgtgaatccc gatattttga ttgtggatga agcattggca gtaggcgatc ttgaatttca
    17161 actaaaatgt atggaaaaat ttacagaaat caagaactcg ggaaagacga ttttattcgt
    17221 ttctcatgat atcagttcga ttcgtcgttt ttgtgatcgt tcctattggt tgaaaaacgg
    17281 agaagtcgtc gaatatggcg atacgatgga agtaacgacg aattatgaaa actttttaaa
    17341 gaaaaaatca gttaaaacag tggatcggaa taaaaaccaa gtagaacggt tcgccgctcc
    17401 agatatcgtc gacgtgacga aagcggaatt gttggataaa gacaaaaatc cggtggatat
    17461 gctggaacaa ggagaaaaac tatacgttaa agtaacttat gaagtaaaag atgatacaat
    17521 caaaaatccg gttttaggaa ttgcgctacg aacagtcgac aatttttatg tatgcggact
    17581 taatactcta ctagatggta tcaagattcc ttggaaaaaa ggagagaata tcttttatct
    17641 ggagtatgaa aagttaggtt tgttagctgg tgaatattat tttgacgttg cggtctttga
    17701 agaaaatgca acggttcctt tggtatataa gacaaagtac atgaacttgt ttgtcagcgg
    17761 ttcttatatc ggagaaggca tcgtggtctt agaccataaa tgggaagaag ggactcatag
    17821 caatgaaata tgattttgaa atggacctag atgaacaaag cagtgtggga aaaattgccg
    17881 cacagatcaa accaggaagt aaagttttag agtttggtcc cggaaacggc cgattaacga
    17941 aacatttaat cggagcaaaa caatgtgaag tgagcattgt tgagttagat aaggaattgt
    18001 tcgattttgt cagcgaattt gctcaagatg gattttatgg tgatatcgaa agttttgagt
    18061 gggcaaacta ttatgctgga cagacatttg attacgtctt gtttgcggat gttttagaac
    18121 atttggtaga tcctggaaaa acattgaaga aagtcagaga atttttgaat gaagaaggag
    18181 agatcctgat tacattccct aacttggcgc ataattctgt catgatcgat ttgttcaata
    18241 accaattgcc gtgggcttct tatggtctcc tagatgaaac ccataattct ttttatactc
    18301 atgatggctt ccaaaaagtt tttgaaaaag cgggattatt cattaatatt gaagattatt
    18361 tgtacttagc tgtaggtgat acagaattaa aatcaactta tgaagaattg ccggaagctg
    18421 tacgctatga tttcaaaatg cgaccatttg gtgaagtcta ccaatacttt ttctcattga
    18481 tgaaacatcc agtagcacaa agttccattg cagagccaca aaattctaat tatgtaaaag
    18541 tgttagaagt aacccagcaa acgaaacaag atgaaacaat ccaacaaata ccattcaata
    18601 atttcacagg ggaaaatgag acactaagtt tccccgtatc agaaacaaca gaaagaatgg
    18661 tgtttcgctt cgcgcaacaa cctagcttta tcgaattcag cgcagaagct gccggagaaa
    18721 aactaacatt tatcgattcg aatgctgtag tcaaaacagt aaatgattgt tatttgtttg
    18781 atgggaaaga attaccagag ttcgttctaa cggatatttc tgggaaagaa gtgactatcc
    18841 attgtcacta tcgctttatc ggggaattga caccaacaat gaaagaattg ttggaaacga
    18901 ttcgtcccat ggctgaagtc acaaagcagc tttctgagaa aaatgacgaa ttagaaagag
    18961 aaaatcatca tcttcttgag gaaaatacaa gactagacca tacgttaaaa acaaccacaa
    19021 atcgctattg cacattgttg gaaagtgatg aatggacaat aaaacataga ctaggacgac
    19081 gtaagaaaga aacttcgaaa aaaatccaag aaaaagaact ttctatatgt attgacgaga
    19141 aaatctggga tgcagaaaca aaaatattaa aaatcatcgg atggggaatc gctaattcgg
    19201 atcgccagcc cttgtcctat aagttatcag ctgaccaatc gccatttttc gaagcaattc
    19261 ctgtttcacg agaagaagtc aatcaagcag aacagctcgc aaaaggaaca gaagcgggat
    19321 ttgagttacg aatcctttgc gagcaagaac gttcattctt agtggaagca gtagctcaaa
    19381 atggccaatc atggatcatt gaaatgtaaa taatgattaa cttataaaga taaggagtaa
    19441 acgaatcgtg caaaaaaaag aaattgccgt atttatcgat caaatcactc gtgaacgggc
    19501 cactggcgat ttgcttattg tgggctgggc aattgatgaa gtgaccaaag aaatccctac
    19561 gataaaggta gaaaaagaaa atgtgattgc tgaatcaaca catgtcgtaa gattggatat
    19621 caatcattta tataatcttg atgttaagac ccaaagcggt ttcaaggtac gtttatcagg
    19681 gaaaatgaga ggaaaagcca tcttagactt ccaaacagcg agacatcaaa atggcatagc
    19741 tgtgaaatta aacggtaaat acccttatga tgacggcatt gaatcttcat gggaaagaaa
    19801 aaaaagatta ctgaaaaaag ggataaatta tgcaaggact catggcgtaa aaaaagcgat
    19861 ccgtcgggtc aaattggaat tgaagccagg atcgatcgac tatgcagagt ggatcagtcg
    19921 tcatgagaaa ccagatttga aagagcaaag gaaagaagca caaggatttg cgtaccgtcc
    19981 attgatttcc atacttatgc ctgtctataa cgttgaaatc aaatggctcg aaaaatgcat
    20041 tgattcggta ctggatcaaa cgtatgatca ttgggaacta tgtatcagtg atgatgcttc
    20101 tactgatcca gctatccgaa agtgtctgga atcctatcaa gcaaaagatg atagaatcaa
    20161 agtcgttttc cgaaaagaaa atggacacat cagtctggca actaattctg cattggaaat
    20221 agcagaagga gaatttatag cactcttaga taatgatgat gaattaccgc catttgcatt
    20281 atatgaagtg gcaaaagtgc taaacgtaca tccagaactg gatgtgatct acagtgacga
    20341 agataaaatc gatgcagatg gcaatcgctt tgatccgcat ttcaaagcag actggtcgcc
    20401 tgatacattg atgggaaata attatatctc tcacttaggc gtatatcgaa caagcattgt
    20461 taaagaactt ggcggtttcc gtaaaggcta tgaaggttct caagattatg atttggtcct
    20521 tcgtgtgaca gaacaaatcc ctgcagatca tatttaccac attgaccgag tcttgtatca
    20581 ttggcggacg atcccaggat caactgcaag taatggcgaa gccaaaagtt atatctatga
    20641 ttccggagtg aaagcattga cagatgccct gtctagaaga aacatcaaag gaagcgtacg
    20701 tcctggcaga atttctggat tttatgagat tgcttatgac gtcttgcaag aggatttggt
    20761 cagtgtgatc attccaacga agaatggcta tgaagatcta aaaacttgtg tggattcgat
    20821 catcgaaaaa acgtcttatt caaattatga aatcattatt gcggataacg gaagtacaga
    20881 tccaaaaatg caagaattat ttgcagaata caagcatcag ctgaaagacc gctttatcgt
    20941 agaattgatt gacattccat tcaattattc tcgaattaac aatttagcag cagaaaaagc
    21001 aaacggcaaa tactttttat tcttgaataa cgatacagaa gtgattgagc cagattggat
    21061 gacggtgatg gtttcttatg cacagtttga tcgaatcggc tgtgtaggag caaaactgtt
    21121 ttatccagat gatacaacgc agcatgcagg agtattatta ggaatcggtg gtgttgcagg
    21181 gcatgcattg aataactatg atcgaacaca ttgtggttat ttcggccgat tggtcattga
    21241 cgtcaattac ttagcagtca ctgcagcttg tatgatggtc aaggcagctg acttcaatgc
    21301 tgttaacggg tttgatgaaa cattagaagt agcattcaac gatgttgact tgtgcttgaa
    21361 agtttatgaa ctgggtcgtt acaatgtcta tgcacatcaa gcagaattgt accatttcga
    21421 atctaaatca agaggttatg aagatactcc agaaaaacaa agacgttttg ctggtgaaat
    21481 caaaaaaatg caagataaat ggccagcata tattgcgcat gatccatttt acaatgataa
    21541 tttaacaaaa gacggaatcg gcgacttttc gttgagacca gattaaacta taataaaaag
    21601 ctgttatgtt gtgattcgct cgcacataac ggctttttat agttttttgt caaaaaaagg
    21661 cagaaggtgc cgactcaaaa aaataagtcg aggttgggac agaagtgttt aactccgaga
    21721 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttcgg cttatttaag
    21781 aagaagttgc ttctgctccc accgtttatt cgtttttaaa gaatgagaca aaagtgtttc
    21841 ttacttttgt cccacgctct tattcatgct cagagcgcca atagatacaa aatcatagcc
    21901 aaagtttcac gaaccctagc tgtcaaagaa tcgatatttt attttaatat aggctatttt
    21961 aaaataaatg gtttaaggta ttcctctaca aaagccactc tatcaattat catgggttta
    22021 aaataagaag tgatggagat aaccagattg ttcttagcgt caatataaag aacatttcca
    22081 ctattaccaa ttgctgaata tatttcgttc tcagaatcta atacccacca taaatagcca
    22141 tatctcatat tttgtaagtc ttctaaagca ctggttctag gtttgaacat ctcgtttatc
    22201 cacatgggtg gtacgatttg ttttccttta tacatccctt tatttaggca taatacacca
    22261 atttttgcca gatcacttgc cgataaacac aaaccatatc ctgccgtccc gatatttagt
    22321 ggatcacaaa accagacatt ggttttagga gacttatcca ttgtaaactg cttatgttct
    22381 tcagctgttt cagcataata attgacatgg tttggtatcc ctataggatc aaatagatac
    22441 ttattggcaa aatcaacagt tgttagctta cttgtctgat acaacaagcc agttaatatg
    22501 tgtaaacaga cagtttgata gttgaattcg ccggttatac cttttcttcc tcccaataaa
    22561 tctagtgatg tttttgtcca atcttcactt gaacagactt ttgtccatgg atcgccttta
    22621 catttgtagg gtgctttcat tgttagcaag tgcttgattg taatagcgta aatagttttc
    22681 tccccgcgtt tcactttata ctctggaaaa taatctaata ctttatcatc gatacttttg
    22741 attaatcctt gctctaaagc aatgccaatc agcaaagata caatactttt cgttgctgac
    22801 atgatatgac aagtatcgtc ttttttataa ccattccatt catctgtata aacttctacc
    22861 ccatccttat atgcgacaat ttgacatata tttggttgtc gaaaaccaat aaaatcatgc
    22921 aactcttgtt tattcataag atattacctc actttcttct ataagcataa tgtcattaaa
    22981 aatcgatttc aattttttcg attcttcacg taatacaggt attcttatgg actagacagc
    23041 gctttgggaa gatggattct ctgaaaaata taaaggttca ctttatgagt ggataaaaaa
    23101 agcagcaaat acttcgactt ctttaattta ccaacattag tgaaaatcga ttcatctgga
    23161 gatttgtaat ttagagtcct tgtcttgatt agaggtgtat aaagcttttt attagacatt
    23221 tttaaattcc attacaattt gcttataaac taggttttta aaggtgacta tggtaagata
    23281 atagagatat tattttgtga ttaggagtgt atatatgaat aaaaatggag aatggagtgc
    23341 tgcacgacgg atattcatta tattaatgga tgttattgta tacaatgcag caatatatag
    23401 tagtttctta ctgaagtttc gtggagagat cccattacga aatttagaaa ctttccaaca
    23461 ctcagcaatt attatttcag tgatttttat tgcgttgaac attttgttgg gtgcatatgt
    23521 tttttataat cgaatgatca gtgacatcgt gtttgtcaca gtcatcggtc aggtattgat
    23581 gacactaggg atcatggtag tgacttttgc gggccgctgg tttgcttttc cacgtcttgt
    23641 tatcttactt tcatttgttc tcggtacgct tttcttaagt atctatcgta ttctcgtata
    23701 taaactttat ttacgcgtaa gtcgagataa aaaggtcgtc attattgggt tagaaaagga
    23761 tgtcgcacca gcaatccaaa attttggttc aaagaaaaac aacaaacata aagtcgaagc
    23821 agtcattctt gataactatt atgcaaacat caaaaatatg attaaagaca tcgatattgt
    23881 ctatcttgct tctcatatcg aagaaactga aaaaatcaaa atctatcaat tagttactaa
    23941 aaatgaaaaa aaattgtttt tgaatacaac atttgaaaac ttgacattga tcaatccaaa
    24001 tattatgaat ttcgaagatg aaagtattat tgaagcatct ggatttagaa tccaacctga
    24061 atatgaacta ttcaaacgtt tgtttgatat catcgtttca cttgttttat tagttattgc
    24121 ttcaccattt atgttattca cagcaatttt agtcaaagtg acatcaccgg gaccaatcat
    24181 ctataaacaa gtgcggatca caaaaaatca aaaggaattt tcaatctata aattccgctc
    24241 gatgaccgcc acagccgaag caaaatctgg accagtttta gctaagagca atgatgcacg
    24301 agtaacgcct gtaggtaaat ttattcgagc tgtccgcttt gatgaattac cacaaatctt
    24361 caatgtgctg aaaggcgata tgtcgatcgt aggaccacgt cctgaacgtc ctttcttcgt
    24421 cgatcagttc aatgcagaaa acccttatta ctacttacgg cataatgttc gtgctggtat
    24481 cactgggtat gcacaagtct atggaaaata tgtctctgat tacaacagta aattacgttt
    24541 tgatttactt tatatcaaaa aatattcatt gatgcttgat ttaaagatct tgctgcaaac
    24601 catcaagatc ctatttgata aaatgtcctc tcaaggactt gaagaagacg aaaatgtgat
    24661 ctcattggca gaaattgctt ttccgaatga aaaaatcttt cgctagttgg gaggcgtaaa
    24721 aatgaacaat ccgaaattca gcataatcgt accagtatac aatgcagaag caactattgg
    24781 tcgaacgatt gatattctaa aagaattgga ttatgaagag tacgaagtcg ttttagtaaa
    24841 tgatggatca acagatcaaa cacaggaaat cattcaagaa ctgattgaaa atgatgaacg
    24901 ttttgaattg attactacga ccaatcaagg gcccggatta gcaagaaacg aaggaataag
    24961 acagtcaaaa ggagaatatc tcttattctt cgatgcagat gacacgccag taaaaacgat
    25021 cttgtcagat tatgatcatt tgctagaaaa gagtccaaaa gcggatcttg ttgttagttc
    25081 gtttgttttt cgtacaatgg acgagggaag aatcgtttcc gaaaaagaaa acttagttac
    25141 agaatgtcac tatctaactc acgaagagtt tataaaagac atgtatcagt taatgaatga
    25201 acagttgatg tatgttgtat ggaacaaatg ctatcgtagc gatattctga aaaataacca
    25261 aattctattt aaaacttata atagttgtga ggatcgaata tttaacttac attattatga
    25321 atactgtcat caagtgatca tgaacccaaa agtagaatat atttatgaat ttgaaggtgg
    25381 gaaggggatc actaatcaat accgacctaa caagttcgaa acatttaaag aattctattc
    25441 attagcaaat actgtaacag acgatattaa taaagacggt atggcggcgc ttcttttaaa
    25501 agggacaact tcagttattt tttctattta tagtacctct gcaagaactt caaaagagaa
    25561 gaaagacgaa gcaaagcaga tattgagtga tccagtgatc cgagaagcca agcaaattgc
    25621 taagacggat tcttcagcaa aaaaaataac aaagttgctt tacaatatgc cagctccact
    25681 atttttatct gctgtaaaag ttggaagctt cgttgaagtg aaaatgccag ggttaatggc
    25741 tgctttgaag cgagtatatt agttaatgga ggaaaatata atgaaaagaa ttttgcattt
    25801 tcagggacga atgggtttag gtggagcaga gtctttcatg atgaatttat atcgtaaaat
    25861 tgatcgtaca aactatcaat ttgatttttt gatttatga
//
LOCUS       DAHHPA010000035        25848 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.036, whole genome shotgun sequence.
ACCESSION   DAHHPA010000035 DAHHPA010000000
VERSION     DAHHPA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.036"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..405)
                     /locus_tag="NBH55_001297"
     CDS             complement(<1..405)
                     /locus_tag="NBH55_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1509292.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQ"
     gene            complement(395..2569)
                     /locus_tag="NBH55_001298"
     CDS             complement(395..2569)
                     /locus_tag="NBH55_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HCD1509293.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2585..4927)
                     /locus_tag="NBH55_001299"
     CDS             complement(2585..4927)
                     /locus_tag="NBH55_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509294.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4949..7492)
                     /locus_tag="NBH55_001300"
     CDS             complement(4949..7492)
                     /locus_tag="NBH55_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1509295.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7502..7900)
                     /locus_tag="NBH55_001301"
     CDS             complement(7502..7900)
                     /locus_tag="NBH55_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1509296.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7981..8265)
                     /locus_tag="NBH55_001302"
     CDS             complement(7981..8265)
                     /locus_tag="NBH55_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509297.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8289..9287)
                     /locus_tag="NBH55_001303"
     CDS             complement(8289..9287)
                     /locus_tag="NBH55_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1509298.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            complement(9280..9627)
                     /locus_tag="NBH55_001304"
     CDS             complement(9280..9627)
                     /locus_tag="NBH55_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509299.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLEGASVVVHEVVIVDKI
                     PKLLLERYGISHE"
     gene            complement(9643..10293)
                     /locus_tag="NBH55_001305"
     CDS             complement(9643..10293)
                     /locus_tag="NBH55_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509300.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(10304..11476)
                     /locus_tag="NBH55_001306"
     CDS             complement(10304..11476)
                     /locus_tag="NBH55_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1509301.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(11495..12367)
                     /locus_tag="NBH55_001307"
     CDS             complement(11495..12367)
                     /locus_tag="NBH55_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1509302.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(12389..13309)
                     /locus_tag="NBH55_001308"
     CDS             complement(12389..13309)
                     /locus_tag="NBH55_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509303.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(13309..13578)
                     /locus_tag="NBH55_001309"
     CDS             complement(13309..13578)
                     /locus_tag="NBH55_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509304.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(13575..13955)
                     /locus_tag="NBH55_001310"
     CDS             complement(13575..13955)
                     /locus_tag="NBH55_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCD1509305.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(14422..15885)
                     /locus_tag="NBH55_001311"
     CDS             complement(14422..15885)
                     /locus_tag="NBH55_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCD1509306.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(15949..18966)
                     /locus_tag="NBH55_001312"
     CDS             complement(15949..18966)
                     /locus_tag="NBH55_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1509307.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(19117..19956)
                     /locus_tag="NBH55_001313"
     CDS             complement(19117..19956)
                     /locus_tag="NBH55_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1509308.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            20101..20346
                     /locus_tag="NBH55_001314"
     CDS             20101..20346
                     /locus_tag="NBH55_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509309.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            20409..20831
                     /locus_tag="NBH55_001315"
     CDS             20409..20831
                     /locus_tag="NBH55_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509310.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(20925..21242)
                     /locus_tag="NBH55_001316"
     CDS             complement(20925..21242)
                     /locus_tag="NBH55_001316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509311.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(21269..21475)
                     /locus_tag="NBH55_001317"
     CDS             complement(21269..21475)
                     /locus_tag="NBH55_001317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509312.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(21456..21638)
                     /locus_tag="NBH55_001318"
     CDS             complement(21456..21638)
                     /locus_tag="NBH55_001318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509313.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(21652..22002)
                     /locus_tag="NBH55_001319"
     CDS             complement(21652..22002)
                     /locus_tag="NBH55_001319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509314.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(22028..22330)
                     /locus_tag="NBH55_001320"
     CDS             complement(22028..22330)
                     /locus_tag="NBH55_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509315.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(22333..22566)
                     /locus_tag="NBH55_001321"
     CDS             complement(22333..22566)
                     /locus_tag="NBH55_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509316.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(23552..23874)
                     /locus_tag="NBH55_001322"
                     /pseudo
     CDS             complement(23552..23874)
                     /locus_tag="NBH55_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23997..25112)
                     /locus_tag="NBH55_001323"
     CDS             complement(23997..25112)
                     /locus_tag="NBH55_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCD1509317.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(25118..25516)
                     /locus_tag="NBH55_001324"
     CDS             complement(25118..25516)
                     /locus_tag="NBH55_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCD1509318.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         7038 a         5444 c         4039 g         9327 t
ORIGIN      
        1 ttgattcaat gtcttttcaa aaaagaatgt ttttctctca atttttttga cgtttctatc
       61 atgaatcacg agaaaacttg ggacactttt aacatggtaa cgttgtaaaa aagtttgaag
      121 ttcttcttgt cttggttctt ttcttgtttc aattcgatat acattcctag agaatttttt
      181 gtcgtatctt tccaagaaag attcaacttc ttggcatttt ttacaatcat ttcttgctag
      241 atacacaaaa aatgattgat tcgttttttg aagttctagt acttcttcta aattactatt
      301 gattagtttt ccatttgatt cttcgttagt ttttatcagt ttaaaacaac caaataagag
      361 gaaactaaca ataagtatcc taacaataac tttcttattt cgcatattgt ttctccaaat
      421 ttggtttccg aatatagacg actcgcggtg gttcctggac gtcttcagct gttcgataca
      481 ccacaaattc gcctgttctc aatgctcgaa tgcgattcgg attgaattta aagcggtcta
      541 cgtctcgttt cgtgcctctt tctcctgtcc aatcaacctt tcccatatca gaataacctg
      601 cttcttgtgt cgtcatttcc gttaattcaa tgtcttcttc tgtccccatt aaatctgccc
      661 aataatcaat ttctgtcggg tcatttgtct tcccaataaa gaacgtattc acattcccaa
      721 tcaactgttt tcgaaggaca ggatcaattc tatcaatgtc cgcaagcgtt tgaggggcat
      781 acgccgcatg caccccagcg cctcgtccct tattgactaa gtctataagg ttatcattta
      841 gataagaagc tggttcgtca aatattacta ggactttcga gcgattggct gtttctcctg
      901 cttgtttata cacttctgac gcaaagaaat taatatctcc cacaagcatt tgagccaatg
      961 tccgaataaa ctcactataa atcaatccgt ttaacgagat ataaacaatc tttccttctt
     1021 taacaacttc ttgaatatct aattctcttt ttcctccaga agtatcaaag agttcaccaa
     1081 gctcactatc caataacata ttgatattgg tctgtaaatt ctcagaagtc gcatacaaat
     1141 aaaacgggtt cttcttatgt tcgtaacgaa caaacaaccg tttaaacaac gtttgctcga
     1201 tttctgtcat gagagaaatg ttttctttta ataatagata atacgtttct aagtcaacgg
     1261 ttttacgatc atgttcaacg attcgctctt cacttaactg ttccacatcg gcaaaatcac
     1321 gttctgtttt gtccatcgct tcattctttt tcgttttttt cttcgtggtt ttcttctttt
     1381 ctacgaccac tttttcttta aataaatctg gatttttcaa ctgaatacga tccgcaaaca
     1441 aatgtaagac gtttcgtggc aataaataat gttgtaacca aactagattt ctttcaattt
     1501 tatggctctt ttggaactcg tctaaaagct gaatcgttac ctgtaataac gctttggctg
     1561 ctgtcgaata atacacgctt tctgtttctg ctagattggt taatttatct cgaatggcaa
     1621 tatcattgcc attttttaca ggattataac gcaaatcgcc accgtccaca aagacttcaa
     1681 tcgggcgatt gtatgcctct gccacttctt ttaacgcttg taaagtctct cctgctcctt
     1741 tcccatcgac taagataacg gggatacgaa acttacacgc atgttctgct agtaattgga
     1801 tcaccgtcgt ttttcctgaa cctgtagtcg ctggaataat cacatgttgg ttcaattcac
     1861 taaagttcat cacataaggg agcccatact catctgttcc taatagaatc actttggcta
     1921 atgaagccag ttgtttattc cgcaaagcaa cgtcttttat gacaattgct acctggtacg
     1981 cttgctgata ctctgctgta tatttttggg actcctgaaa tttattcttc cgacgttcct
     2041 tgtattcttt actatttagc aaagctgttt gccgttcttt ctttgaaaca acttttcttt
     2101 tttcaataaa agacgcatac gcaatcaaac aacttcctgc acctgtccca cctaaaagga
     2161 gggcaaaaat cgtttgtcca ttaaactgca acgcatgaga aaacaagttt aatggaaata
     2221 ataagtaaca cgacaaatac cctctaggaa gaacaaaaat aaaacaaaat agatttaata
     2281 gcgtaaaacc taacgcaaaa ataaaaaaaa gagagcttct tcgttttaag aagctctcat
     2341 actcaaagtc ataaatatgt gcacgtgtct cttttttatc tatcataaag acaatcgcta
     2401 aaggaatact gcctacaata aaaaatggat atagacaaac acctaataaa gtgacgataa
     2461 aataaagttg ttgaaaccga ctttttggcg tttctttttc ttgttttctt ttgcgatcga
     2521 cagaaaacat ttgctcaaaa tgtgtcggtc gttttggtgc attcatcata ctatcgattt
     2581 cctcctatcg ttttcgttgt ttcatttctc ttcgataaat cggttgtttt tctcgttttc
     2641 gtttcatttt tgcttgagtt gctcgaaact cactccgtgt cttcggtgct tgttttggtg
     2701 attttgtttg tttcatggtt gagtatgctt tagatacggt tgaaggcgct ttggtttgtg
     2761 ctgttactcg attgactgct gacatatgtt cccgaacccc ctctggacgt cgaacagatt
     2821 tcggtgtggt agaacctgtt gctcgtggta cattcccttt tcttagtggt ttttgtaccg
     2881 catgtttttg tcctgcaact gtccgggttt tcatcgaact tgtttttgtt acgggagtaa
     2941 gtgcctcctt ccgacgacga cttgtttgtc cctgacgacg ttttcgatcg ttcaattctg
     3001 ttctaggcgt atgcttctgg ccataagact tgtgtttttg ctttggattc gctaatgaca
     3061 ccaccattgc agaaccctga ttgcgatacg gactacgaga cgcacctttt ctacttcttt
     3121 ctcttagact aggattgcct tgcaattctc gttgtaataa atcggctgtt tttcttgcct
     3181 gtttctccat tgctgttcgt tgcatacgtt cttgactttg ctcttctgct tgtttcgctt
     3241 gatcatgcaa gtctcttgct ttttgtgcca tacgttccat acgatttggt gctttatgac
     3301 gaggttgttg tgcggattca cgccgaaaag cacttcgtgt ggcttgtttc gccgtttgtg
     3361 gcatacgaac gtctgagcca cccccacgtt ttcgttgacg ttcttgtctc ttccgtgcgt
     3421 tttctttttc tttttgtttc tgtgcttttc tcgcttcttt gcgttctttc aaccgttgtt
     3481 tggcttccct tctcaatttt gcttctgccc caaatggatt tcccgcaaaa gctaacgctc
     3541 ctcgaacacc cattcttcca gtaaatagac tgcctaaaaa gcctcggaac aaataaattc
     3601 ccaacggcgc aaacaaataa acaatgatca agagaatttt ttcccatgga tcgtcttgtg
     3661 tactacttaa ttggaacccg taactaagaa aagaagaaaa aaggatacat gcaaaacctg
     3721 tcattccttt taaaaacgtc aaaataccaa aaactttggc atagttacca aacacattca
     3781 ttgtgacaag aaacaatcct aaaaaaagta agaacggcaa catcggtact aacaatagtt
     3841 gcatagttcc aataatcaat cgaaacatac ttaagacaaa ataaacaacg gtttgaatca
     3901 gattcacgac aaaataaaac aaggcaaatg ctgtattttt ccaactattg taatattgta
     3961 tgttcttgtt tttcagtgta tctgcttcat actctgtgac tttgtcatga attttctccc
     4021 ctatttttgt acctaaatca ttgtccaata aaagattgat tcgatcatat tcttttccag
     4081 catatttgac tttcttctca cgaattgtgt ctacattggt tgtgccataa tttaacaaca
     4141 gatatggttc ataaacatta ctataaaaga cccgagccgc catcaatttg cctgccgttt
     4201 ccactttttt atccgctgtt acctcttctg tttcttttgt ttcataattt gtttcttctt
     4261 caggtgttaa cactggattg accttcacga acgccgtttc cactgtagaa tctaaagaaa
     4321 aaagaacatc aaaaagattc ttatttgtcc cagaatcttt taatacgctt aacccagtaa
     4381 aaataaggat cgtcataaga aagacacgga gtgcttgttt cattcgagcc tctccagcat
     4441 atttgattgc catcataatt gccgaaaaac tgattccaat agttcctgca atacttaaca
     4501 tattgtccgc aattttttgt gttaagtttt gaatgggttc tcgaatggcg gaaataatat
     4561 ctaattcaaa gagaaattca accattgaag cattgaattc tcctaacatt tttacgccac
     4621 tccaaacaaa atttttcatc actcctgcaa cattgtacga tagctttttt ttaaggttca
     4681 ttgactcata gtcattggtc atcaattcaa acgaattctt tttgtaattt ttatacaatt
     4741 gatccacatc agtcgttact tcttttactt ctactttatc gttggcattc tctccttcag
     4801 aacgatcggt ttcagtgaca cccgcaccat cattcatttg atcggcagaa acagtagccc
     4861 cttgtagaca gaaaaaaaga ccccctaata aaaggagcgt ccaccatttc agagattttt
     4921 ttcgcaaaaa aaccacctct tttttgtttt atcgttgttt ttcattttct aacattgctt
     4981 gaattttttc atcttgattt tcagtagaag aaacagccaa taaccaacta tcaaacaaca
     5041 catcaatggc aatcggttga tttcttccca aatgatcctg gaataaacag gttcctgttt
     5101 gtaattgttc catcatttct aaattggctg aattcacttc catatcaaag aaggacaata
     5161 gtttctctcg tgcttctttt tgtttcggac gaaacgcaaa tttgtaagaa attaactctt
     5221 ttttatcttc ttgatcgtaa tctttaaacg cttgcacaac aaggtaaata tccgtattat
     5281 tcgcacgtcc ttttcgcaag ctatcatcaa tcaaatagct tccttgtgcg gtatcttcat
     5341 acccttttgc ctcgtcataa ataatcgcag cgtcttcttc tttgtttgta gaaaagatat
     5401 acgtaaactt cataatgact tccataattg ccattcctgc aagttgttcg ctatttaatc
     5461 gacccgaatc aatctctttt tgtgtcggaa tgcgtattcc ttgtgtccct aacacgttga
     5521 tttgtgaatc aaaacgtatc ggcgtactgt gctcattccc aatgagaatc tttcctaaac
     5581 caacgctatg ccctaaaatc aatttagcta gttctttgtc actttttcga atttcttcga
     5641 taacttgact taacgaatgc ttacctggca tggtgtaaac tacatcgatg gcgtcttgga
     5701 tcaccgtttt cttgtggctc gctgtttctc gattggtatc tacttctcct aacattccta
     5761 acacattttt agcggtttga attgcctctt cttttggtaa aaacaataga ggatcaagca
     5821 tccctcgaca gctttcatct gaagataacg aaataaaatt aattcgttca tacatagcca
     5881 caaactctgg tatgtgtccg aatttttcaa gtgcctttaa aaagaacttt tgggtttcat
     5941 ttttcggatc aatataaaga attttttgac ctaaaaacat cagccataaa aaaatatatt
     6001 ttacaaggac cgattttcct ttacctggtg gaccaataat caacgtattt ccattggtat
     6061 gttccgcgcc ttcaatactt ttctttgtta aattgggata ataccatact agtttactag
     6121 atagcgctaa cgcttctttc gcagatttaa acttttttgc aataacaatc cgtcctaaag
     6181 gaaatccttt cttatttcct acttctttct ttaagtccaa gccataatct gccacaaaac
     6241 ctgtcgtagc gacctgctcg taactacgaa aacgttgcgt acttccaatt aagcattgat
     6301 taaacaacgt tagctgatcc gctaccgatc gataaaattt gaaatctgta gactcaatcg
     6361 caaattcaat atctttgact cgttcttcca attcttcttt tgaagaagca gatacaacca
     6421 gcgtgacggt agaataacac aatcgttttt ctttgcgctt cagcttatct tttacatcca
     6481 ttaaccgttc tgtaccaaaa tttaatacat cgtctgaatc taaaatgccg tcaacggtct
     6541 catattcttt ttcttgttca taaatccgtc cagccatttt attaattttt cgttcatcag
     6601 attttgaatg ctcaaatcga agtcgaatat gcgtatcaat tggaaaagat aagctatcct
     6661 gaagattttg aataaagcta ctcccataca tggctaaagg agcgtccgtt tgacttaaaa
     6721 acgtcgaata atgcgtttga ttcaactgtt caatcgcaat gtatcctgct tcattcgaaa
     6781 tgacgccctc gcaaatttcc attggcgtaa gctgtctttc aggcaatttt tcttctgatc
     6841 gatggaaaaa ataataataa atttgtccaa tctcttgggc agataagcgc tctatcggct
     6901 tataattgga caattccgta tagagctcct tttctaatgt ttcatacgtt ggtaacacac
     6961 tacttatagg aactcgttgt cccgtaatac gatagataaa ctttttaaaa acatgcttaa
     7021 gcaattgaaa atattcgatc ggatcagaca ctttcatttc tgtagtgaaa cgcacaaaca
     7081 gatacgttcg atactcgttt aattgcactt cgtcttttaa aatctttcct gcctgttcaa
     7141 agtacacttt cccaagatct gcgcattctc cctttaccaa atctcgaatg gtttcttcaa
     7201 tgtgcgtatc taggttaaat tgacgtggaa tattgataaa atgatagtca tattcatcat
     7261 gtccaagtat gccttcgcca tcttgaagat agttcttgaa aaaatcaatg tcatttaaag
     7321 gaaatacttg ggaagaaagt tgatagccta cccacacatc atcatttttg gtcaaaacaa
     7381 aatttcggat aatggcgtct atcggaaatt ctaatcgagg aagcttcgtt tttttctttt
     7441 tgaactgcca aatcccgata ataaagacga ccaaaagaaa caaaaacgcc atttactcga
     7501 ctcactcctt ccatttgtgt agggaatacg tcactttttc atggaaaaac ggcacataca
     7561 tttcatgttc aaagcgttgt ttccgtttct gataatagga cgttcgccca attaaatact
     7621 cgaaaaagtg tttattgtcc catttcattg aaaacaatgt ccaaacgaga acaccaatcc
     7681 caaaaatcat aatcaaccag gactttcgaa acagtgtctg caagaaacta atttgattca
     7741 catacgcaat caaaaatagg atcaaaagaa tgaagagaaa taccccacct atgactaatt
     7801 gttccatgcg aatgccatta gaaatccagg gaattttttg tcctttaatc ccgtaaattt
     7861 tgtgagggtg cttaaactcc tttgtatagt tgtattccat gttcactcta cctcctttta
     7921 caaaaaaaga agcgaccaga agttctaatc ttctgatcgc ttttgcagtt tgtctttttc
     7981 ttatgataat tgtatccagg gcacaatcat ttgcgttacc cccacgttct cttcactatc
     8041 cttaataaag ataatgttct ttagccattc accaatatca acggcattcg ataacaataa
     8101 gacgagcatt atcaaaataa agacgccaat aatggcaaac gctgcccgcg caaagccttg
     8161 ggtcgcataa gcaccaacca ttagaacaat cgcaacgatt gccaatagcc aaatgacttg
     8221 tccagataca tcatcaaata acgatttaag atcaaaaagt ttcatttcca ttctcctcta
     8281 agttcatttt attcatttaa cgtagtaaca aaataactat cgccacttgt ttttacttcc
     8341 attgaaaagt ttgattggaa ctcgtttcct ttctcaaaat agaaacgata cttcccttgc
     8401 aaaaagtagt ttccttcttt attttttact gcatgatcca gttgtacatt gatcaattcg
     8461 ccgccttcta accctttttc gttagaaatc aatgccaatt tttcatcatt tttgacatat
     8521 aactcaaaaa accgtttcac aaatgcttcc agcttgattt cttcgctttc agtgaccgct
     8581 tgcccagaag aataaaatcg attttcctga tagttcacag aattggtatt cttactctca
     8641 gaaagattca aaaattgaac gccatcaagt acttttaaaa cattgttttg ataactgact
     8701 ggaaaagaaa gttgactgct tttccaatgt tctccttctg taaaagtaac ctcataaaat
     8761 aggcgataca ttgcttgtgt caaatcgatt tcttctagtt taataaagcg aacatctttc
     8821 gctactcgat tggtagccgt cgttacaaag cctagatcag acgcttccaa tccttgtgct
     8881 aaataatgat tgacggaagc ctcccattcg tttcgttttt ctggcgaata agtaaataac
     8941 ctttcaataa aatgttgtcc ctcgtacttc aattgttccg tagaagacaa ggtttggttg
     9001 acttcagtta acaatgtttc tggattgact tgttttttca ctgctaaacg cgttaatgta
     9061 tccactcgtc caaagtttag gaggagcacg acacccatta acagaaacaa acaaaaaaaa
     9121 gcgcctgaaa atacactcat ggcacttttt tgcgaaaatt ggatcattcg atctgacggt
     9181 tctgttttct ttttttggaa caaaaaacgt ttcttttgtt tcttttcctt ctgttgtttc
     9241 ggtggcttag tttggaacgc ttgtggatta aattttgcat tactcatgag aaatgccata
     9301 ccgttctaac aataatttcg gtattttgtc cacaatcact acttcgtgga caaccacact
     9361 tgccccctcc aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa
     9421 agcatgacaa atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat
     9481 ccgccacaaa acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc
     9541 ataagaaaaa gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac
     9601 ctctcttatt gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg
     9661 ttgattaaag ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa
     9721 ggttacgtca aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata
     9781 cgacaccaca acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca
     9841 tttctctaat tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata
     9901 ttgcttcatc agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga
     9961 atcaggctca taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg
    10021 aatgtttcct ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt
    10081 cgtgacctcg ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg
    10141 tcctttcttc tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt
    10201 tagctgtagc ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc
    10261 gcctacacaa acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc
    10321 gagcaaaaca aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt
    10381 cacctacacc gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc
    10441 aatcttccgc taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa
    10501 tgataatatc gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc
    10561 ctcttgcgtc gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc
    10621 ctgctttcgt taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg
    10681 gattttcgac actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca
    10741 gactttttgc tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta
    10801 gacaataatc ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag
    10861 cgacgggatc actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac
    10921 gattccccat cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt
    10981 cctctacgct ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat
    11041 gaatcaaaaa ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa
    11101 aatttttcaa agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg
    11161 gatcgtttgg tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta
    11221 acatacccgc tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa
    11281 tttgaagccc gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt
    11341 cggttggact acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa
    11401 acagaaaacc aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa
    11461 gaagtttttt cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc
    11521 ttttgcgcca aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa
    11581 cgagagacac ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca
    11641 ccgcaatatt tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc
    11701 ctgattcttt ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt
    11761 caattggtgt acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt
    11821 gaacattttg tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt
    11881 cctgtactgg aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag
    11941 tcgtatttcc ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac
    12001 catttcctgg gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat
    12061 ctggattttc tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg
    12121 ttggtggaat ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt
    12181 ctcctccatc tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg
    12241 gtggaataat ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg
    12301 cactggttcc taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt
    12361 ttttcatgtg tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg
    12421 gaagcgtgga aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg
    12481 tttcttgtgt ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc
    12541 cttctttctt catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt
    12601 gtttcgcaac tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca
    12661 tttcacccga ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt
    12721 ttgtcgcaag ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta
    12781 gttgttcaat ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga
    12841 cgataaatcc aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc
    12901 tttgtgtaaa cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg
    12961 gtcgacgttc gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta
    13021 aggtttgaat aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt
    13081 caatcaatga aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg
    13141 gaatttcatg gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca
    13201 atagctcttc attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat
    13261 ctgttagtaa agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac
    13321 ttcttccacc acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac
    13381 ccgatattct tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt
    13441 atattcgttg cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc
    13501 aatctttgga aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat
    13561 tcctgttact ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt
    13621 gtcggtaagc aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa
    13681 aaaataaatg cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac
    13741 gggacaagaa ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt
    13801 tcagaagcat tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg
    13861 gcattcatta acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag
    13921 ctattctgcc aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt
    13981 tttgattcta gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa
    14041 tacaccacca gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa
    14101 tttcgccata aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt
    14161 tatgcaatat atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa
    14221 taaaaatcgt ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct
    14281 aatactatat ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa
    14341 aatgagagat acaaatatat caaatattta aaaagtaacc acattttcta taccactcct
    14401 tatcagaaac agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt
    14461 tgttaaacta ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt
    14521 tgaacccaca taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata
    14581 ttcctgttgg tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt
    14641 tcctgacaaa gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg
    14701 ttctccttga ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa
    14761 ggaactagaa attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg
    14821 accagctact tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt
    14881 tttatcgtag ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata
    14941 ggtttttgcc ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc
    15001 ttttaacagt ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt
    15061 tgcttgggtc gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc
    15121 ctttccgtta tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg
    15181 actttgtccg cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc
    15241 attttcttgt ccaattttac gagcagattc accgatttta cggataaacg tttctacaga
    15301 cgcgtctttt tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc
    15361 ttgtatcggt gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg
    15421 tgcctgcggt acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg
    15481 ttccgttttt gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga
    15541 gctttcatca gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt
    15601 gtcttctggt ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg
    15661 agcgttcatt tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg
    15721 attttcttct ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt
    15781 tgtcagttga ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg
    15841 acctactaat aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt
    15901 cttcaatcaa tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg
    15961 tttcatttta cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc
    16021 aattaacaca tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt
    16081 cggcgtttgt ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt
    16141 ttgtttttca ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt
    16201 atagacaata tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc
    16261 aaaggatact ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc
    16321 aacgactgta tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt
    16381 ttcccctacc gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt
    16441 gtctggatca aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc
    16501 tttcgcagtc actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc
    16561 ctcttctttg tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat
    16621 gtctgctttg tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt
    16681 ttcttttacc gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt
    16741 cacccaatat ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac
    16801 taattcttgg tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt
    16861 agaaatttct ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg
    16921 ttgtacttct ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc
    16981 ataatgctca gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt
    17041 ttctggttct tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc
    17101 ttcaatcagg aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa
    17161 gtctacttca cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg
    17221 cgtcgtgtta tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta
    17281 gttgtctggc acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt
    17341 tttgtttgcc ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt
    17401 tttcacaagg actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa
    17461 ttcgatattt tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc
    17521 cgcattatag acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt
    17581 tgcagttaac gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag
    17641 aatataccca gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc
    17701 gtctttttca tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt
    17761 atattgtttt gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt
    17821 tttccctgtg acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat
    17881 agttagtcca gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg
    17941 atctacatag gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga
    18001 aattcgtccg tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct
    18061 tacttgaacg ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt
    18121 tgtatcctta acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga
    18181 aggatcttca agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt
    18241 agattgtcca atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt
    18301 ttgtgctaat acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa
    18361 agaaatcttt tttctaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata
    18421 ttgaataccc gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat
    18481 ttgccagcga accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat
    18541 attcacatct ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt
    18601 cttttccaag attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa
    18661 agaagtcgct ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac
    18721 ctcttgagtc attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc
    18781 ttctgttgtt tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc
    18841 agtcttcatt acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac
    18901 agatcctaac atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt
    18961 aaacatattt ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg
    19021 gacttcgaca ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat
    19081 tctagtcttc atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt
    19141 ttgtgacccg tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga
    19201 atttggatcg ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac
    19261 ctttccacta ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc
    19321 accatttccg ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc
    19381 tccacctgtg cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg
    19441 ttgtgtaggt gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc
    19501 accagttgtt cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt
    19561 ttgtgtattt gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc
    19621 ttgaatcact gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc
    19681 attaaatact aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc
    19741 cgctaatttt tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca
    19801 gacaattgtg tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt
    19861 ccaaactttg ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac
    19921 tacgattcct agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata
    19981 aatttattta caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag
    20041 atttttttat tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac
    20101 atgcaactta ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt
    20161 aaacaagctg ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga
    20221 aataatatca aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta
    20281 tatctaagag aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt
    20341 caatgaggcg ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg
    20401 gaggttctat gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg
    20461 ctattattgc tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat
    20521 cgactactac aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag
    20581 aaacgcatgg acaagattta attgatcttg gttcttctca aattactact gtcttatttg
    20641 aaaagaaaaa tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca
    20701 aagtaacaaa taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca
    20761 agttaaagat tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact
    20821 cactgaaata agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat
    20881 cattttataa aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc
    20941 tcaatttctt ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc
    21001 tccccttctt ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga
    21061 taccaaccaa caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat
    21121 tcatttttcc atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca
    21181 caaatttcgt catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc
    21241 atcttaactt ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt
    21301 gtttactagt taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag
    21361 cgtcgttttt ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt
    21421 cctgcatttc ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc
    21481 attccctcat tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc
    21541 acttgtccta taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg
    21601 aaactaatgg ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt
    21661 tgttatttct tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa
    21721 aatttcaata aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca
    21781 ataaaattct ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat
    21841 ttttgaatca aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg
    21901 gagtgcctgt tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc
    21961 ttttatttca ttgttctcaa atactttata actttttttc attgttagtt cctctttcat
    22021 tcctctctca attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa
    22081 tccttaatgc tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc
    22141 ttttagcagt tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat
    22201 tcgaaccgat tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag
    22261 tgattgctcg attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt
    22321 tagacaacat ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa
    22381 tccacaaata aatgaattca tattccaagt accattccct tcaactacaa cttttctcct
    22441 gtcctctttc ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa
    22501 tttgtttgta taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat
    22561 tgtcatttcc tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca
    22621 acgaatatac ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc
    22681 ttttacttgt tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt
    22741 ttttcatcca ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt
    22801 ttcttgttca tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt
    22861 catcaatcat caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc
    22921 ccaaactcta aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca
    22981 tcatatgttt ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac
    23041 ccatccatac gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag
    23101 tccctgttag acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc
    23161 tcccaaaaga aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact
    23221 tgagtatttc tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat
    23281 catcaaatgt tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt
    23341 cgatagcgat tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa
    23401 aactaacaaa caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata
    23461 gttgttagtt taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca
    23521 attggggcaa acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc
    23581 gctgtggtcg ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt
    23641 ttgttttcct ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg
    23701 aattaatagg tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc
    23761 ctggttcgaa caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg
    23821 agaaaaaaac agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct
    23881 tttccctgtt gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg
    23941 ccgattaaac aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat
    24001 gtgtccaatt cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa
    24061 atctgcgttt ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct
    24121 gcttgatctg atagttttgt tgccattgga tctagattca attctcttac acaacattcc
    24181 caaaaattca atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca
    24241 tgacgagcaa ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca
    24301 aatgtttccc ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac
    24361 tttcgttgcc gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc
    24421 gcttcacgcc attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac
    24481 gtactttcgc tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga
    24541 ctagtcgtat cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta
    24601 aataattgtg cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa
    24661 ttatcaacag attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca
    24721 ggacctaatg aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct
    24781 ttgtttcttt ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat
    24841 tctttcaatt cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct
    24901 aattcttgtt ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttcgttga
    24961 atggctaaat ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt atcagacaat
    25021 gagcgatatt tttcacttgt aataaatacc tttggaaatt gatagtataa ttcattgtat
    25081 ttacgagttg cggtaaatct ttttgttgcc atcgttatta cacctcatct tgtccatata
    25141 ttaagaaaga attttggtgt tggtagacac gaacatcttc cattgtagca atcaattctt
    25201 ttagataaat tcgaccgaat tcaattgtta gtaagtcgta atcttctaat tcatttttga
    25261 tttcttcaat ttcttcttca gacacattga gtaaaatggc taattcttga ttggtataag
    25321 aaatatagac atgtttcttt tcatcaatcc gtttttcaat taaggattct tctaagtact
    25381 cattcagtaa gctataggcg tattttgctt gtgcagtcat cgcataatat tttgacgatt
    25441 ttataaagaa tagttttggc atatgaaaag ttgcttgtgt cagcacatct gttgcgcgaa
    25501 atttgttttt taccatattt tttcctccta gaaaaacacg caaaaaaagc tctgtataaa
    25561 tcgaattaca cagagtttct tcttgttgat atttaatttc taaaatacgt ttcgggtaaa
    25621 aactaaacac ttactaatat ttagattgta gaatcttcgc cttcatttat tcattttaaa
    25681 gagaaaaaga tcaagtgaaa tgtgcccaaa agtttgattc tattaatttt taaacaccat
    25741 tcataatata tttcctataa tgtaaaaagc gtcgatactt attaaagcat cgacgttatt
    25801 tttatagtaa atttatcaaa atttttgcca acattttttc cgattttt
//
LOCUS       DAHHPA010000036        25198 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.037, whole genome shotgun sequence.
ACCESSION   DAHHPA010000036 DAHHPA010000000
VERSION     DAHHPA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.037"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            414..1385
                     /locus_tag="NBH55_001325"
     CDS             414..1385
                     /locus_tag="NBH55_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCD1509319.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1463..1699)
                     /locus_tag="NBH55_001326"
     CDS             complement(1463..1699)
                     /locus_tag="NBH55_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD1509320.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1668..1799)
                     /locus_tag="NBH55_001327"
     CDS             complement(1668..1799)
                     /locus_tag="NBH55_001327"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD1509321.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(1875..2258)
                     /locus_tag="NBH55_001328"
     CDS             complement(1875..2258)
                     /locus_tag="NBH55_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCD1509322.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2291..3076)
                     /locus_tag="NBH55_001329"
     CDS             complement(2291..3076)
                     /locus_tag="NBH55_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCD1509323.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3254..4354
                     /locus_tag="NBH55_001330"
     CDS             3254..4354
                     /locus_tag="NBH55_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD1509324.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4344..5168
                     /locus_tag="NBH55_001331"
     CDS             4344..5168
                     /locus_tag="NBH55_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCD1509325.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5165..5980
                     /locus_tag="NBH55_001332"
     CDS             5165..5980
                     /locus_tag="NBH55_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCD1509326.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            5977..6807
                     /locus_tag="NBH55_001333"
     CDS             5977..6807
                     /locus_tag="NBH55_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509327.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6814..7845
                     /locus_tag="NBH55_001334"
     CDS             6814..7845
                     /locus_tag="NBH55_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509328.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(7952..9225)
                     /locus_tag="NBH55_001335"
                     /pseudo
     CDS             complement(7952..9225)
                     /locus_tag="NBH55_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9502..9711
                     /locus_tag="NBH55_001336"
     CDS             9502..9711
                     /locus_tag="NBH55_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509329.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            9761..10477
                     /locus_tag="NBH55_001337"
     CDS             9761..10477
                     /locus_tag="NBH55_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCD1509330.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10379..10899)
                     /locus_tag="NBH55_001338"
                     /pseudo
     CDS             complement(10379..10899)
                     /locus_tag="NBH55_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11172..13643
                     /locus_tag="NBH55_001339"
     CDS             11172..13643
                     /locus_tag="NBH55_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCD1509331.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(13746..15482)
                     /locus_tag="NBH55_001340"
     CDS             complement(13746..15482)
                     /locus_tag="NBH55_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCD1509332.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(15479..16183)
                     /locus_tag="NBH55_001341"
     CDS             complement(15479..16183)
                     /locus_tag="NBH55_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1509333.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            16314..16817
                     /locus_tag="NBH55_001342"
     CDS             16314..16817
                     /locus_tag="NBH55_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCD1509334.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            16777..17115
                     /locus_tag="NBH55_001343"
     CDS             16777..17115
                     /locus_tag="NBH55_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCD1509335.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            17136..18554
                     /gene="ffh"
                     /locus_tag="NBH55_001344"
     CDS             17136..18554
                     /gene="ffh"
                     /locus_tag="NBH55_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCD1509336.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            18666..19244
                     /locus_tag="NBH55_001345"
     CDS             18666..19244
                     /locus_tag="NBH55_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCD1509337.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            19248..19769
                     /locus_tag="NBH55_001346"
     CDS             19248..19769
                     /locus_tag="NBH55_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD1509338.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(19848..20402)
                     /locus_tag="NBH55_001347"
     CDS             complement(19848..20402)
                     /locus_tag="NBH55_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD1509339.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            20655..20930
                     /gene="rpsP"
                     /locus_tag="NBH55_001348"
     CDS             20655..20930
                     /gene="rpsP"
                     /locus_tag="NBH55_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCD1509340.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            20942..21193
                     /locus_tag="NBH55_001349"
     CDS             20942..21193
                     /locus_tag="NBH55_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCD1509341.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            21318..22109
                     /locus_tag="NBH55_001350"
     CDS             21318..22109
                     /locus_tag="NBH55_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509342.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            22279..22800
                     /gene="rimM"
                     /locus_tag="NBH55_001351"
     CDS             22279..22800
                     /gene="rimM"
                     /locus_tag="NBH55_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCD1509343.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            22790..23551
                     /gene="trmD"
                     /locus_tag="NBH55_001352"
     CDS             22790..23551
                     /gene="trmD"
                     /locus_tag="NBH55_001352"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCD1509344.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            23687..24034
                     /gene="rplS"
                     /locus_tag="NBH55_001353"
     CDS             23687..24034
                     /gene="rplS"
                     /locus_tag="NBH55_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCD1509345.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            24292..24585
                     /locus_tag="NBH55_001354"
     CDS             24292..24585
                     /locus_tag="NBH55_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCD1509346.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         7806 a         4678 c         5443 g         7271 t
ORIGIN      
        1 aatctgaaat aaaaaatata cagccctgtg gtactttacc tacaaggctg tatatatttt
       61 ttactctcag cgtttttgac tccgtgtctg actccgtttt attgattttg aatgttattc
      121 aatgaccatt taacaggtca aatttgatgt ttttgattcc gtatgacgcc gtttgatatc
      181 tagtgatata gtgatttgag tctacatcga ataagggaat aaaccaataa aaaatgattt
      241 agaatattaa aataacaaaa aagaggacta cctgtattag tcctcttttt tgaccagatt
      301 ttgaccagtc tattttacat cctagtatat ttatatgagc taattttatg attaaatggt
      361 aagtttttcc ttgtgtgttt tagtttaaac tagtgtgtaa ttcaaaaatc tatatggaag
      421 gaacagtatt aagaatagaa cagggttctc tacatgatgg tgctggttta cggaccgttg
      481 tttatttaaa gggctgccca ttacgctgtg cctggtgttc gattcccgaa tcacaaagca
      541 agcagattga aaaaggattt ggccagacca tgaccgctga agaggtaatg gatgagattg
      601 agaaggatgc tgttttttac tttcactcgg atggcggagt gaccattagc ggaggcgagg
      661 cactggttca ggcggatttt gctaaagaaa ttttgcaaaa atcgaaatac attggaatca
      721 ataccgtact ggaaaccagc ttttgtggtg cttataatga aatccaaaag gtagctccct
      781 atgtggatac attgtttgtg gatgtaaaaa tgtttaccag caagctccac aaacaatgga
      841 ccggcttaga taaccaacaa attttgaaga atattcgtcg ctttttaatt gagtatccta
      901 actgcgaagt gcgaattcga gttcctgtgg ttccaggtat caatatgaac ctgaccgagc
      961 tgctgacgat tgcttgtttt gtggcagatc ttgatcggtt cgtaccgtta gaattactac
     1021 cgtaccactg ctatggtatg cacggttacc aagcattggg actggagtat ccgttggcag
     1081 acacgccagc gccttcacct aaagaaatgt ttgcattggc agatcaactg gctcgcacgg
     1141 ttcctcattt gctggtcaca gctttaacca aaacatttat ttatttattg aatccaagct
     1201 ttatcttatg gataaatgaa agacactcac tagctgcagc cagctcaaga atagagcttt
     1261 tgctgaagtc agtgagtgtc tttttacgat gttttttcga agttaatttg tcgaatgaac
     1321 actggctcaa tcggtacatc ttcactgttt gtcggctgag ctgcgatcgc tgctacgatt
     1381 tctaaccctt ggtatacctg tccaaaaaca gtataacgac catccagctc cggtagtcct
     1441 ccaagctcca agtatgcttg ctttacatta gcaggtcagc ctgcgccttt gtgttttgca
     1501 caataaaaaa ctggttgccg ttggtgtggg ggccgtggtc ccctaaggcc aaggcgccat
     1561 taaaatgata aagtccgcga tgcacttcat cagcgaaaaa gccctcatag ctggaaacag
     1621 cttcatcacc agaaccatcc aaagcgccac cttgaataac aaattcctta atgacacgag
     1681 ccgaaaggtg tgttgtcata aacgccttgc tcagctaggg agcgccagtt ggctacagcc
     1741 aggggagctt ggtcaggaaa taagcggaga cgaatttcac cagcatcggt gaccagcaca
     1801 caaacttctt cctgctgcgg tttttctaat tgaaagagag gaaggtttgc aactgaaacg
     1861 ccttgatcct gccatcaaat atcgaaaatc cgccactcga ccttcgcctg atcaccaagg
     1921 cgagccattc ccaataaaac ggtataggcg gtttcatccg cttgaaaggt caattgcagc
     1981 tgtcctcgta aatcgttgta gtcatctggg gcggctactt gataaaaaag atgaaactgc
     2041 gcatgcaaat attccaaagg ctgatcttta aaggctgcaa aataagcctg aactttttcc
     2101 cgctgcaagc tgtcagccac caaaaaattt tccagttgtt gttttggaac ggctgctaac
     2161 aaggatcggc aaaccgcctc aattttcaac gcatcccaat gtcgcgcaat taaataatgg
     2221 atgggtgcct cttgattgta gtcccacagg ttgctcattt ttttcaccac tttcttctat
     2281 tatataggaa tcacaaaatc ggcagcggcg tcaggcggcg ttcttttcct tgaatgacta
     2341 gcaacttttc tacctgacag tctgccaaca gctgactcat ttttttaaag cccagctgat
     2401 aatcttcggc aacatgagca tccgagccta aggtaaagag ctttccgccg acactttgat
     2461 acaaaggaac ggcataacgg taaaggtctt cgttgccgta tttcaagaag ctcttggcat
     2521 ttaactccat cgccaattct cgttcaacaa cctttttgaa aatcgttagc agctgagcct
     2581 caaaatgctc cgcgaattct tccgctgtga aggtgaagcg gcgtaatcca taatcaaagt
     2641 gagtcaaaat atctccttct ggaaaatccg tcaagacctg caacatctga tcaaaatatt
     2701 ccttggtagt ttgaatggga tctttggtta aaaccacatc gtccatataa tcaaagcggc
     2761 cattttgatg aatacttacc agctttaaat cataaggatg ctgagcgagg taatccacga
     2821 tccgctcttc ttgtcctgga acaacgccaa tctcgatacc tcgcagaaat ttggtggaat
     2881 aggtttcttt taaagagtcc agtttgttta tataggctgg ataatccgga atgtcatctt
     2941 ttccggtaga tggattgttt aaatcaaagt gatccgtaga aacgaaaaac tccggctgat
     3001 aggctaaata gttttcaaat ttttcctctg aatcaaagga aaaatacgtg tggagatgct
     3061 gatcataata ctgcataggg agcctccttt tttctactat cataccgcaa aagctttaga
     3121 aggttaacgg gaattcgtcg aaatttacaa accatataca acctttacat tgttcatgaa
     3181 accttttcaa gaaactgatt ctctcattac aaagctttgt tagaatggaa ttatcgaaag
     3241 gggagaagaa tgaatgaaaa tgaatgaaaa aattcgtaac atgactatcg gtttattttg
     3301 tgcagcaaca gtaagtatcc tgctgacagg ctgcgtctca ggtgaagcaa cgaagcaatc
     3361 ggctgcggca gacgactctt tagatacgat tatagaaaaa gccaaggaag aaggagagat
     3421 tgccagcttg ggaatgccgg atacttgggc aaattgggta ggcacttgga acgaccttga
     3481 aaaagaatac ggcttaagtc acgtggatac ggatatgtcc agtgcagaag aattggctaa
     3541 gtttgaatca gaaggaaaaa atggaaccgc cgatattggt gatgtcggta tcagcttcgg
     3601 tccgttggct gaaagcaaag agttaacctt accttataag accaactatt gggaggaaat
     3661 tccggattgg gcaaaggatg atgatgggga ttggttgtta agctatacgg gaaccatttc
     3721 ctttatcacc gataaagaga atgtgaagca agcaccgact agctggaagg agctgcaaga
     3781 gggtgactat caagttagcg tgggggatgt gacgattgcc aaccaagcac aatttgctgt
     3841 attggctgcg gcgattgcca acggaggtga cgaaaaggat attcagccag gtctgaatta
     3901 ctacaaaaaa atggcagaaa aaggccgttt gtccaccgtt gatgccagcg tagcaaatct
     3961 tgaaaaaggt gaaatcgatg tggcagttgt ttgggacttc aatggactaa attatcgtga
     4021 ccaaattgac tccgagcgtt ttgaggtaac tgtgccaaca gacggctcca tcattagcgg
     4081 gtatacaacc ttaatcaata aaaatgcccc tcatcccaat gcggcaaagc tagcacggga
     4141 atatatttta tctgatgaag ggcaaatcaa tttggcaaag ggctatgcgc gacctattcg
     4201 caaggtagag ctgcctaaag aagtagctga aaaattactg ccggaggatg cttatgaagc
     4261 ggcggtgcca gttgaagaca atgaagcttg gaacaagacc tctgtagagc ttccagagct
     4321 ttggcaggcg gaggtactga cctatgcaaa gtagacttga caaactgatt gtagttatac
     4381 tagacggctg tcgctacgac acagccgtcg cccagcttgg gttcatgaat catttagttg
     4441 agcaccagca agcaagctta ttgaaggtaa aaagtgagct gcccagcaat tcacgtccgt
     4501 tatacgaagt actgatgacc ggcgttccag cctatgaaaa tggcatctat agtaattacc
     4561 atgctcagcg atccaaagag tggtcgctgt ttgaattagt taaggaagct ggcggaaaaa
     4621 caggagccgc cgcttaccac tggtacagcg agttgtacaa tcaagccccc tataatcgtt
     4681 tggttgatcg catccagcat caggaggagc gactgattca gcatggcatc ttttatagtg
     4741 aggacactta ccctgattcg catttatttg cggatgccca ttatttagtg aatcagtatc
     4801 agccagactt tttggtcgta cattcgatga atattgatga tgtcggtcac aaatttaccg
     4861 ccgattctca tgaatatgca gcagcggtta atcaggcgga cagattattg gctatttacc
     4921 ttcctgaatg gctgcaaaaa ggttatcaag tcgtggtgac tgcggatcac gggatggatg
     4981 cgcacggact gcatggcggc agcttggctg cacatcgtga agttcctttt tatttgattt
     5041 caaataaacg ccaaaagctg aaggaacaaa ctgtaccaca gctgctggcg gctccattgt
     5101 tttgctggtt attaggaatt gcaccgtcag agaaaatgca ggacattcaa gaattgatga
     5161 ggggatgaaa gagatgaaaa aacgcttggt tctggttcct gcctccttta tcttctgtgc
     5221 atttttactg ctgccgctgc tttttatggt tctgtgcagc ttgcacagtg atttaactaa
     5281 ccagtggaca ttggccaatt acagtgatat tttcagcaac aaatattaca cacaggcctt
     5341 tgttaacagc gtactgattg ctgtcagctc cagtgtgatt gggttaatcg ggacgtttat
     5401 tttatgttta tgcttaatga atctatcgac taaattgcag gagaaaatca cctttgtttc
     5461 caatttggca gctaattttg ctgggattcc gctggcattc tcttttatta ttttgttggg
     5521 gaatgtaggt gtgctgaaaa tgattttgcc atttctagcg gacttcaatt tgtattcttg
     5581 gtggggattg accatcacgt atacctattt ccaaattccg ttaggggtct tgtttttgta
     5641 tcccagcatg aaggaagtga aaaaggaatg gctggagtcc gcacaattgc tgggcgcttc
     5701 atcagcgtat gcttggttca aggtggcttt gccttttttg cgaccatcga ttgccagcac
     5761 ctttgtcatc ttatttgcta atggcatggg gacctatgaa acagcgtatg ccctgaccag
     5821 cagtaatgta aatcttttaa cgattcgaat tgcggcttta gtgtcgggag atgtgttcgc
     5881 caagcctaat ttaggcagtg cgttggcagt cgctttaggt gtcctgctgg taacggcaat
     5941 gttgattatt caaaagaaag gcaaggtggt gcagacgtga aaaagttaat aaaaccgatt
     6001 ttgttctgct ttgtcctgta ttacttgctg ccgattattg gaacgatttt gtatgccagc
     6061 tccactaaat ggtcaaaatc gcttttacct agtgatttta ccttgcaatg gttccaacag
     6121 ctgctgacgg acagggaatt tattgctgcg gtgggtcgct cacttttatt agcaggtgtg
     6181 gtcttggtga cgattctgtt gttgatgatt cccaccatta tttggattca tttgtatttc
     6241 cctcgtttga atcgttggct ggaaaagctg ctcctgctgc cgtatgcttt accaggagtg
     6301 attttggtaa cggctttgct tcgaacgtat gctgaaactg gtatcccaat gtttgtagtg
     6361 ttggtaggtg cattgtttat tacggcgttg ccgattgtct atcttagtct taacaaccag
     6421 atgcgcttaa ttaatctgaa ggaattagtg gatgcagcag aaaccttggg cgccccgatg
     6481 tctacgatta ttatccaggt gctgtttcca aacatccgta tcggtgtgac cctagtttct
     6541 ctgatgattt tttcttcggt ttttggggag tacatgctga cgaacctgtt gattggcggt
     6601 cgatttcaga cggtacgcat ctatatgctg cggcggatga acgaaaacgg ccatttggct
     6661 agcgctgtga tggttttgta tttgctgttt atggtgggaa ttgctttaac tacctttatg
     6721 ctgacttatc gacaaaagca acagctgtcc cttaagcaga cgagggaaga gaagcagtcg
     6781 aaaaagaagc ccttaaagga gttgtgacgg aaaatgtttt tagaaataca tgatctcaat
     6841 ctgttttttg atgaaaaaca gattttagct attgatgaaa ttcagattga aaagggtgag
     6901 ctggttactt tactaggtcc cagcgggtgc ggaaagtcta cgttgctgcg ctgtatcacg
     6961 ggattggaac aaccaaagag cggtgagatt gtgctggaca atgagaatat tgccgataag
     7021 cctacgaagg atcgcaatat cggctttgtt tttcaacagt atgcattgtt tccaactatg
     7081 actgtttttg aaaatgtagc ctttggtttg aaggtaaaga agctgagtcc tgaggtgatt
     7141 caggaaaagg tttttgagat gctatcattg gtggatatga cggagcaggc agataaaaat
     7201 gtccagtttc tttctggcgg tcaacgacaa cgggtggcac tggctcgctc attggtcact
     7261 gaaccgaaag tcctgctgct ggatgagcca ttaagtgctt tggatgcccg aattagaaaa
     7321 cagctgcagc gggatttgcg cgctatccag caatcgctag ggatgacgat gatttttgtg
     7381 actcatgacc aagaagaggc gatgcgaatc agtgatcgga tttttgtgat ggaagctggg
     7441 cgggtggctc aggtttccac ctcgaaggaa ttgtatcaga atcctcagag ccgatttgtt
     7501 gcggaattta tcggcaacta taatcgtttc gaatggtatg agctgaatca gtatacccac
     7561 gagttttccg ctaagggcaa tcgctgcatt tattatttaa gaccggagct gattcaattt
     7621 gaaccggttc ctcagggggt taagattccg gttctatgga aggaatcatt tattttaggc
     7681 aacattcaac ggtatgtttt tcaaacgctg gagggcaaag aaattttagt ggatcggttg
     7741 aacgatcggg aagagaaaat tagcgatgcc ttatacattc agctgaaaga tattctagca
     7801 atccctgtgg aaattgatac tcagcatttt gcacaacaaa gctagtgtat agtcggcaaa
     7861 catttctggc tctttgtcaa ataggactga tagagtgata taagaacatc taaggtagaa
     7921 ttgattttct gctttagatg tttttttatg tttaataagt gatcagatga ttgattaaga
     7981 aaattctggt tctcatccta acccgtaagc tattcgtttc aatgctttga tgtgggtatg
     8041 cagattttct attttgccat ttgagtaagg caactctaag gctaaacgaa tgccggattc
     8101 atagtttata aacgcttttt ttatatgatg cagttctctt ggaaccgact tgggcattgt
     8161 ttttacaagc tgaaagaaga tgtccgcttc tttgtcataa aaagcttgaa gaatttcttg
     8221 atagtaagca taagctattc gtagttcatc agaagtagaa agtagacgtt ctacgacttc
     8281 actttcggtt aaataggccc aaccaaaatt acgccatttt gtccatttat cataaacaag
     8341 cgtgtcctgt cgtttgatca gaagtcgaga taaagccttc acttgtcgat agtgtttggc
     8401 ttgtattgga tcgtttcggt cgaagctttt catgactcat agtcaaatat ccttaaaact
     8461 gcgaatgagg tgcttgacga tgtgaaagcg atccacaacg agtttggcat gaggaaagca
     8521 ctcttttatc agcaaagcat aagaggcgtt catatctgtt acgagtattt ttacgtttaa
     8581 acgtgcacgt cgttcaaatt gcataaaata agaaatgaga tctttcttct tacgtgaagg
     8641 aagaatatcc agtaatttcc cagtatcgcc atccatacaa gaaaaagaat agggaccgac
     8701 ttgatttttc aatgaacgaa attcatctac caatgaacag cgaggcaacc aatttatttt
     8761 cacgtttgtc atggggagca gagaagcgag tgttcgcgaa acggttgacc aagaaacgcc
     8821 gcaatgttta gaaatcgtcg tcattgcttg tttttcagtc aattcctcca atattttata
     8881 atgtacttgt ttggcgataa aacagttttt ttcaacaatg taggtattcg ccgtaaaata
     8941 ggtgtgacag ccacgacaga ggtaccgttg ctttgccaaa gcaagataag tccgttgatt
     9001 cgcacactta ttgagaagga ccagcgattt tttctttccg ttcttaacga tcagcccatg
     9061 ttgattcacc tgtttacact gaggacaggg ctgaatcgtt ttaatgagcg tggcagaaac
     9121 aacgatactg ttgattgaat gtattttttt cttctcgaca tgagtgattt gaatgtttcc
     9181 atctttgatt cctagtaata gtttgataag atgaagatca ggcatacaga acgctccttt
     9241 gattgtgttg tggtgattca attctaacgg agcttctgta tgttttgttt ttttatgctc
     9301 aaaaggactg atagaatgat ttactatcag tcctagaaat tatagcgcca catttctttc
     9361 acctttgact tttcagacgg cagtttttat gctgatactg tagagagagc aggacacagc
     9421 ttttgcttgt tttgggtaag ggggaaagaa gatgaccaaa gcacttattt ttgattttga
     9481 tgggacgatg attgacagtg aatggatgtt ttacgagagt attcatcact atttactgaa
     9541 gcctatttac tggagcagtt cggctataaa attccttttg cagactatca acaaggtgtt
     9601 gggacggcag cagaactgtc cttttatgaa gggataagtc aagcggttgg gagaccagtg
     9661 gatgctgaac attttgagca gcggatcagc agcactggaa aatcggagta gtctcaattc
     9721 tgaactgcgg gagctagact atttttttga agcacatccg atgattaagg aaaaaattga
     9781 ttcctttgtg acgatcgatg atcttcaggt cggcaaacca gatccagaag gttatttgtt
     9841 gtgcttgaaa cggctgaggg ttgaacaggc tgttgtagtt gaggatagtc cagtcggtgc
     9901 ccaagcagca ccgcatatga cagttgatgt acaagcgttg gatgcagatt ttttcgcctt
     9961 tagcgggcat aaaatgtgcg ggccgacagg aatcggtgta ttatatggca aacgtcaatt
    10021 gttagaacaa atggagccag tcgaattcgg cggtgaaatg atagattttg tcaatcttta
    10081 cgatagttca tggaaagagc tgccatggaa atttgaagct ggtactccga acattgcagg
    10141 agcaatcgat tacttgaatg agattggtat ggagaatatt catcgttatg agcaagaact
    10201 ggttgcctac gttttaccta aattacaagc gatcggcggt atcaccattt acggaccgca
    10261 agatcctgaa catcatactg gcgtggttgc ttttaatttg gatggcatcc atccccgtga
    10321 tgtggcgact gctctggata tggaaggtgt cgctgtacgt tcaggacacc attgtgcaca
    10381 acggttcatc gacgatttct gtttgactaa ttggtcgttt aaacagtcga tgataatgcc
    10441 gtttgagcgg ctccaatgtc gtggaatctt tgagtaagag tttccaatat cgcttgagcc
    10501 gacggtactt cttctgattt tcagattacg tgattgattt tcgtacagtt ctatgagaat
    10561 cgcctgcttc agttcctttg ttagttgatg atgttcatca actaagtccg ttttcgcaga
    10621 aaaggtctgc ccacaatcat agcatagaaa acgggtacgt tttaattcta aaatagtcaa
    10681 cttttctttg actttcaaca tctgagtacg tgtcgtatag gaaccgttgc ggataatacg
    10741 aatcgatcca cagtgttcac aatgggaagg tgagtcatcc aatctccctt taataaactg
    10801 cgcttgaacg cctttatatt ctttttcaat cagccattct tcatcaaaaa ataaatgttt
    10861 atctgttaat ccgagtaatt ttctagtatg attatccatt gagagactcc tttggtagga
    10921 tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga
    10981 aaaagtgtta gtagaaaatc gccttcgatt ttccaccaac acaaaatatt atagaaccct
    11041 ttttttttgc tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt
    11101 ccatatctgt tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc
    11161 gaggtagcca catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa
    11221 gatcatcaga taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc
    11281 aagtcaagga acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa
    11341 caaatcagca aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct
    11401 tagccatttt gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca
    11461 tagccaatac aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac
    11521 taaatgtttt aaatacagca aaagtcaatg tcatacgaga cgggatagaa caaacaatag
    11581 atatagaaga ccttgtaata gatgacattg ttattttaga gactggatat caaattccag
    11641 cagatgcaga ggtcgtggaa gggaaactgc aagttaatga ggctttattg acaggcgaag
    11701 cagatgaaat aaccaaagaa ataggtgatc cattaatgtc cggcagtttc attgtttccg
    11761 gaaaagctta cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag
    11821 tcaaagccaa gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat
    11881 tgatcaaatg gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt
    11941 acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg
    12001 gtatgatacc tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta
    12061 aactagcaaa gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg
    12121 taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa
    12181 aaatgatcgt ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca
    12241 ttgctgacta tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat
    12301 attttacaaa aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa
    12361 aattcagcag tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt
    12421 tgcgcgatca attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc
    12481 gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag
    12541 tcacaccatt aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat
    12601 ttgattactt caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca
    12661 ccgtatccaa tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat
    12721 ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg
    12781 tcaagccaga acaaaagaaa atcttcgttc aattgataaa gcgtaacaat acagtagcta
    12841 tgacaggtga tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga
    12901 tggcttccgg aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact
    12961 tttcacgcat gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat
    13021 cagccagctt gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga
    13081 tctttgctct gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta
    13141 tcggacttcc atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat
    13201 tcatcatgaa tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg
    13261 gtgcgttggt gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat
    13321 ctaccatgct tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga
    13381 ataaattcag aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct
    13441 ttttgcataa gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca
    13501 ttctattttt ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt
    13561 ttgaacgtaa aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat
    13621 cgatatttaa acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc
    13681 actcgtgtat ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct
    13741 cttttttaca ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct
    13801 acagtaatcg taccattaag gagttgcacg tagtctttta caatcgctaa ccctaatccg
    13861 gtcccgcctg aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt
    13921 tcctgatcct caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct
    13981 gttgcctgat agcggataat aatctcgctg tcgctttttg aatattggac agcattttca
    14041 atcagatttt tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt
    14101 ccaatcaatc gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt
    14161 tgctggaaat aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt
    14221 gacagctgga tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc
    14281 tcaagaaaag aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta
    14341 aaaccaatca gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc
    14401 ttttgtagct tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt
    14461 ccggtacctt ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct
    14521 gtaagggtga tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga
    14581 atgagttttg gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg
    14641 tttaaatgta gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc
    14701 aggaatacgc caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta
    14761 cacgcccgat acatcttact catttgttcg ctcaacgcat taatcgtctg atacaattct
    14821 tcccattgat cgggtgtttg catgattact tccgcctgat taggattttg caccattttt
    14881 tttagtacgg gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc
    14941 attcctgcaa gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc
    15001 tcagttctcg gtaaaaaaac gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg
    15061 ccactttttt tgactggtaa tgcgacatat aaaagatcct cattcaaagt agtacttttg
    15121 cgcaaagcgc tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt
    15181 cgctgttcat tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt
    15241 aaagtaatcc gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct
    15301 gcttggaggt tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag
    15361 ctttcttgct gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca
    15421 aaaaacaaac taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc
    15481 atcgtttggg ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttgggt
    15541 gttttggatc tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc
    15601 tttgtcctgc gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac
    15661 ggtcttttcg tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca
    15721 gatggtcgtc tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata
    15781 gatattcagg ctcagcttca tcttttgatt gcttacgagg ttcaattcgt ctgaatattg
    15841 ctttcatacg ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac
    15901 ctatttctag gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg
    15961 gtgtttcatt tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa
    16021 gcatcacatc taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc
    16081 ctccatctgt ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg
    16141 ttacaataga aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat
    16201 aggtttcatt ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga
    16261 aataaatggt ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg
    16321 gaatcagtgc atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac
    16381 cagaattgat ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg
    16441 gcggattacc aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt
    16501 ggaaagatca agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa
    16561 atggcgcgat caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga
    16621 aggaaaaaag cccttcatgc ggaacccatt cgatttatga cggaagtttt tccggaataa
    16681 aaaaaaatgg tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct
    16741 ctgatgaaga atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga
    16801 atgaacgcgc tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg
    16861 gaattatatt atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc
    16921 cgacaagcag tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa
    16981 aaactccatc ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca
    17041 tatattacag ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa
    17101 gcaatcgaag aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac
    17161 gtctccagca ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa
    17221 aagaaatgat gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag
    17281 tcaaagattt tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt
    17341 tatcacctgc acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt
    17401 ctgaaacagc agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc
    17461 ttcaaggggc aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg
    17521 aaaatgctcg tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat
    17581 tgaaagtatt aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc
    17641 cggtagagat cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat
    17701 tgatcgatac ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa
    17761 tcaaagaatt gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag
    17821 atgccgtcaa cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca
    17881 ctaaacttga tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg
    17941 caccaatcaa attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag
    18001 atcgtatggc tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc
    18061 agcaagacta cgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat
    18121 tcgatttcaa tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag
    18181 atctaatcaa aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag
    18241 atccaaaaga tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag
    18301 caaatcctga tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca
    18361 gtgttgttga agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc
    18421 aaatgtccaa aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca
    18481 aaggaaaact aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga
    18541 aaaaacggaa ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg
    18601 agcttttcta tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg
    18661 cacagatgag atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg
    18721 tctggggtgt tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta
    18781 atcaagccgg attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat
    18841 atgatcgatt cgatatcaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa
    18901 tggaaaacaa aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca
    18961 aagcagtctt gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt
    19021 ctaatggaaa aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc
    19081 ctctttcaga tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca
    19141 caacaatcta tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt
    19201 tccgcaaaga agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaaatca
    19261 tattttatgg tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat
    19321 ggctttttga cactccaaca ggtaagcaaa ctacttatga agctttttat gaaacaatcg
    19381 atacgaccat catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag
    19441 caatttatcc agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag
    19501 atgctgaggt cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag
    19561 gaaaagatat ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa
    19621 tgattgatga atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat
    19681 tccaaaaagg aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat
    19741 ttatcgaatt gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg
    19801 cagaaatgtc ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat
    19861 ttgttagaaa aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca
    19921 acgatattac cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc
    19981 tgcaaggttg gcacattcaa aaagacaagt gattggcgta gatggtggtt tgcgccaaag
    20041 ccgccgatcc cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg
    20101 tgtccataag gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg
    20161 ttgtattcag gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga
    20221 gtccattcag caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca
    20281 tcgattttta caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct
    20341 acttttttgt tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc
    20401 atacgatttt tcctccttca aactatctat aataatctta caaaagataa gtaactggag
    20461 caaataaaaa cacttactga caaaagctac atagaagata gattacttat ttttaacaaa
    20521 aaagaagtct aaaatcttct attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa
    20581 aaaaagcttt acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga
    20641 ggtggaaata aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc
    20701 cgttttaccg tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa
    20761 ctgtaggtac ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag
    20821 ttcttgactg gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa
    20881 aagaaggcgt tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga
    20941 tatgaaagat ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga
    21001 tcaagtcaag ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc
    21061 tcctgaagat attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac
    21121 aatcgtttac ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga
    21181 taaggaaaag taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt
    21241 ttattaattg aagaaatgat tatttggcaa aacaagcaag aaagcgtatc cttataggag
    21301 gagggagtga acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa
    21361 gcgcttgtag ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg
    21421 aaactgttac cagtccagaa aaaagtagta ccaaaacggc ttccaccact gaacaaacaa
    21481 tggctacctc aaataatgag aaaaagactg ctttagacca gttgaaagaa caacagccaa
    21541 atgttcccat gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac
    21601 atacaaagca aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg
    21661 ctgacgaatt gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt
    21721 ctagtcaaga aacaatccaa gttttgcagc catttgagat cgacacaaat ggtcagcaag
    21781 tggacttagg ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg
    21841 aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc
    21901 ccttactatt agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg
    21961 aacaatttgg taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa
    22021 gctggcaaga accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga
    22081 agatggcagt ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta
    22141 actccaagtc acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt
    22201 aataccttaa aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa
    22261 aagaaatggg gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa
    22321 ggtatcaaag gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa
    22381 aaaggaacgg tgctaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa
    22441 agtcatcgca aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat
    22501 gatgtggaaa aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta
    22561 gctgaaaatg aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact
    22621 gagttaggta aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa
    22681 cgtccgaaaa aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat
    22741 ttagaagctg gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga
    22801 tgtattgact ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa
    22861 ggctgtagat aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa
    22921 caaacatcag acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt
    22981 ccagcccatc tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag
    23041 agtaattctt cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc
    23101 acaagaagaa cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg
    23161 cacattagtg acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg
    23221 tgcgatggtc atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac
    23281 ttcagctcaa acggattctt attcaaccgg tttattggaa catccccaat atactcgccc
    23341 ggcagaatat aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat
    23401 tgaagaatgg caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt
    23461 ggaaacactt gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga
    23521 acaagaaaaa acagcagatg aagccaagtg actttttcta aaatcttctt tacagtaggt
    23581 tgataatatg ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc
    23641 tgtgagaaaa gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat
    23701 cgaagaatta acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac
    23761 tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg
    23821 tgttgttatc aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc
    23881 taacggagtg ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga
    23941 agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg
    24001 aaaagctgct cgtatcaaag aaatccgtcg ttaatcattg ttatcagact gagaaagaac
    24061 ccttggtatt caagggttct tttttcttag catgtaattt gaacaccctg atccagtttt
    24121 ttctttaatt attggtaggg gagaataatg gattagaata aatctaccta taagtagtag
    24181 taaaaaaaag agcactatga taatatcttt cttaagagta accgctttaa taaaaaatcc
    24241 agaatattct aacataaatg gagtattctt agaatcaaaa ggagaaagaa gatggtttat
    24301 acttatacgg agcaagaaaa gatgaaagtt ttagacacat tcatgaatga tggaaaagta
    24361 aaacaagtac caagttcaac aaaaaagaaa tacatcctat tgaaagaagt cttgaaacgc
    24421 tttgaccatg gagtgactta tacagaaaca gaggttaatt cgatactgct gaacgtattt
    24481 tctagtggcg attatgtcga acaaagacgt tatctaatca catttggctt tttcaaaaga
    24541 agttcagacg gtcgggcata tcagatgatg ggaattgaaa attagaagtt ttaatttata
    24601 ctcattgaaa aaagttcaag tatgcgatat tggttctaca ccgcatactt gaactttttt
    24661 tgtcatctgt atctataata caaagccaag aacgttattc aaaatatgga tagctatgat
    24721 aaaagctaat gagcgattcg cagttaaata agcaatcgtc agtaaaatcg ttccaggagc
    24781 gtacatgaaa aaatcaattg gatgcgttac atgaataaag ataattctct tttatttgta
    24841 cgaataaata ccaaaacgta cctcctaaaa ttagattttg gacagatgcc ctttaaaaag
    24901 ataacaagat ttcgttcttg aaacaatata tatgctttta ttccaatgag gttgtgaaaa
    24961 cagtattttg tcctgagcgt tagaaaattg aaaatgggga atagcaactc gtactttacc
    25021 attttttaag tgaatacaag atcagctaat tgaacaccgt ttattcgtga tgagggacac
    25081 aacactgcag tcgacacatt tctcttttat gttcaaagat aacaaatatt taaaacaaag
    25141 aaaaatagta gataatcccg tcctaactct tttattcttc cctcatagct tccaaatt
//
LOCUS       DAHHPA010000037        24776 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.038, whole genome shotgun sequence.
ACCESSION   DAHHPA010000037 DAHHPA010000000
VERSION     DAHHPA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.038"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..405)
                     /locus_tag="NBH55_001355"
     CDS             complement(<1..405)
                     /locus_tag="NBH55_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCD1509347.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISC"
     gene            627..1700
                     /locus_tag="NBH55_001356"
     CDS             627..1700
                     /locus_tag="NBH55_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509348.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            1706..2392
                     /locus_tag="NBH55_001357"
     CDS             1706..2392
                     /locus_tag="NBH55_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509349.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            2400..3047
                     /locus_tag="NBH55_001358"
     CDS             2400..3047
                     /locus_tag="NBH55_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCD1509350.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(3120..4511)
                     /locus_tag="NBH55_001359"
     CDS             complement(3120..4511)
                     /locus_tag="NBH55_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1509351.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            4768..8157
                     /locus_tag="NBH55_001360"
     CDS             4768..8157
                     /locus_tag="NBH55_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCD1509352.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            8160..9581
                     /locus_tag="NBH55_001361"
     CDS             8160..9581
                     /locus_tag="NBH55_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCD1509353.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEELRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            9578..11455
                     /locus_tag="NBH55_001362"
     CDS             9578..11455
                     /locus_tag="NBH55_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCD1509354.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            11547..12389
                     /locus_tag="NBH55_001363"
     CDS             11547..12389
                     /locus_tag="NBH55_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCD1509355.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12541..13416)
                     /locus_tag="NBH55_001364"
     CDS             complement(12541..13416)
                     /locus_tag="NBH55_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1509356.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            13623..14654
                     /locus_tag="NBH55_001365"
     CDS             13623..14654
                     /locus_tag="NBH55_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1509357.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            14743..15048
                     /locus_tag="NBH55_001366"
     CDS             14743..15048
                     /locus_tag="NBH55_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCD1509358.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            15227..17242
                     /locus_tag="NBH55_001367"
     CDS             15227..17242
                     /locus_tag="NBH55_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1509359.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            17244..17528
                     /locus_tag="NBH55_001368"
     CDS             17244..17528
                     /locus_tag="NBH55_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1509360.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            17554..18927
                     /locus_tag="NBH55_001369"
     CDS             17554..18927
                     /locus_tag="NBH55_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCD1509361.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(19010..21025)
                     /locus_tag="NBH55_001370"
     CDS             complement(19010..21025)
                     /locus_tag="NBH55_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCD1509362.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(21047..22261)
                     /locus_tag="NBH55_001371"
     CDS             complement(21047..22261)
                     /locus_tag="NBH55_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1509363.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            22482..23284
                     /locus_tag="NBH55_001372"
                     /pseudo
     CDS             22482..23284
                     /locus_tag="NBH55_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            23580..24524
                     /locus_tag="NBH55_001373"
     CDS             23580..24524
                     /locus_tag="NBH55_001373"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD1509364.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         8392 a         4521 c         4666 g         7197 t
ORIGIN      
        1 acaactgata tccttatact tcagaacatt ttcatcaaag agacctagcg agcgtttcaa
       61 taatgctttc acgcgcatga tcagttcttc tcgatagaaa ggttttgtta gataatcatc
      121 tgcacctcgt tcaaatcctt caatcttatc ttccaaacta tcttttgcag tcaaaatcaa
      181 tactggagtc tgtatttttt ctttccgtat ttttgctaac acttcatatc cattcatttc
      241 cggcatcatc aaatccaaga ttgcaagatc ataaactcca ctttccaact cgaacagtcc
      301 ttcactacca tcataaactt gtgttatctc gccgattgca gcgagtattt cttttatatt
      361 attggataat actttatcgt cttctactac taatagttta atcattttcc cacctcattg
      421 atttggtcac tttagtttat catggaaagc ataaataagg cttaaataaa ttttttatga
      481 ctcatgttaa ggttttatcg aaagatgtct tagacaattt attgataaga aaaaaacaac
      541 acaagtgaca aattgtcact ttagagttac actattcaac aagtgacatt ttgtcactat
      601 caatctatca tagggaggaa aaacaaatgt ttctagctat caaagaaatt cgctattcta
      661 aattacgata tggcttgatc gtcggtatta tgttcttgat tgcgtatgtc gtattcatgt
      721 tgtcaggact agcgaatgga ctttcagaag agtttaaaaa agcaatcgat gattgggacg
      781 cacaagagat tgttttgtcg gaggatgcaa atcaagtatt tgcagcttca caattaacaa
      841 gaggagatct gaaatacatt gaaggaggag agaaagcacc aatcggattg tacagcggag
      901 caatcaaagg aaaaaacaaa gaaaatgtta cagttttcgg cacaacgaaa gaagcatttt
      961 tattaccaaa actcacgaaa gggcatgaat ttaaaacaaa gaatgaaatc atcatctctc
     1021 aaaatctggc tgattcaggc tataaaattg gagatacgat caaaattggc agttacgacg
     1081 atcctttgac gattgtcggt atctttcctg aaacttatta cacagtttct cccgtcatct
     1141 atgcgtccct agatacgtgg actgctttga agtatggaca acagccgttt gcttctgaag
     1201 aagaacagcc gatcaatggg atcgtaataa aggattcagc taaaatttcg aaagaaaaag
     1261 catcgaaggg tctacagtta ttaactattg ggacattcat tgaaagtatt cctggctatt
     1321 ctgcccaaaa tatgacctta aatgcgatga tatacttttt gttcttagtg gttgctgccg
     1381 tggtcggtat tttcatgtac gtgatgacac ttcagaagac tgctattttt ggagtaatga
     1441 aagcacaagg gatcaaaaat ttcttcatcg ctaaatcttt agtagcacag tcctttattg
     1501 ttggggtggt aggcgttctg cttgctcttt tgtttgctta tttgactagt ctgattttac
     1561 caagcgcgat gccttttgct gttttttggt cacaatggtt gctatatagt gggattttga
     1621 tcattgtcgc aatgctaggc ggattgtttt cgatacgtac gataaccaaa gtagacccaa
     1681 ttacagcaat aggaggttaa taaaaatgaa aaaaatatta acgatgaaaa aagttaccaa
     1741 aacatttggt tctggtcata caaaggtctc agctttgaaa gcaattgatt tttatgtaaa
     1801 ggaaggagaa ttcgtcagca tcattggtcc ttctggttca ggtaaaagta ctttccttac
     1861 catttcagga ggattgcaaa caccgacttc cggagaaata gtgatcaacg gtcactcact
     1921 aactgagctt ccagaaaaaa aacgagcaga tttacgcttt aaagaaatag gatttattct
     1981 ccaaagctct aatttgattc cttttttaac ggtaaaggaa caatttgaac tagttgacca
     2041 agttacttca aaaaaagaag attcaacaaa aatcgattcc ttgctgcaat ctttagatat
     2101 ccagtcactt aaaaatagtt atcctaaaga cctttctggt ggagaaagac agagagtggc
     2161 aattgctcgc gctttatata atgagccaag cttgatctta gcagacgaac caacagctag
     2221 tttggataca gatcatgcat atgaagtcgt caaattacta gttaaagaag ctcatgagaa
     2281 acaaaaagcg actattatgg ttactcatga tgtccgtatg attcaatgga gtgatcgcgt
     2341 ttatcgaatg gaagatgggt acttgacaga agaagagaaa aagaaggaat gaatccttca
     2401 tgacaatgaa gggctatcca cgtataatca gagataaagg agagatgtgg atgccaaaag
     2461 aaactttttt caatttatcg aacgcaaaga aacagagaat cgatgaccta ttactggaga
     2521 tattttgctc acagcacatc agccaagtca aagtctcaga aatcgttgac aagatgggga
     2581 tgtcgcgcgg tgctttttat aaatattttc aagatttgga agatgcttat ttgtatacga
     2641 tcaaaagcag ttctcacata gtccatcaag atatcttaaa attcattagc gaaaacaaac
     2701 aacatttctt tttggggatc gaaaaatatt tagagtggtg ctgcgactta gatcacgaaa
     2761 gtatctattg gcaaaggtta tacttgttaa cccagcgaaa tgatcagcag acaaccaaac
     2821 gtagagaact atcggctgat tctccaatga acgaaaaatg gctggaatta ttaagaatca
     2881 atcatttctc cattcaatcg tcggaagaag cagttagttt cctatacttt tccatgaccc
     2941 tcgtaatgaa cactttgacg gatctcattg caaataattg gacaaaagaa gagttgttaa
     3001 aagactttcg ctataaatct cgttggattg ctgaaggaat acaataaaaa gaaacagcta
     3061 tgtgtatttg tcctgaaatt tttatcaggg tggtttatac atagctgttt tttgattatt
     3121 cataactcga ataagcggat gagttcttct tcatttgcga atgacacact atcatcaatc
     3181 aatatatgag gaacatgggc attcaaaatg ggttgattcg taacgatcag atcatactcg
     3241 agaagattgt atttcgacag ctgatcttca gtaatagcag ttgtctcaga gtgaatttta
     3301 tgcacagcaa gaatatcact gatatattct tcaatatatt gactatgtcg aagacttaag
     3361 tcgctgataa tcagtatttt tttatcagaa gtataatggt gaatttcagg attaccgata
     3421 agcatccaaa agatatagct agataaataa taaatcagat tctgattcag tagagtggaa
     3481 taaaattgaa gttctgcacg aaatccagca tatagacgta tctggcggtg ttgcatctgc
     3541 tgtgcaaagt aagttgcacg gatcgtcaaa tgagtcgtat cgtatgggaa aatacgggag
     3601 aaaaaataaa tttgcgttaa taattgaaca acatattctt cgtttttttc tttgcaatca
     3661 gcggatgaaa acgaacagta ggacatattt tttcgtatct gctcactgat ttcatttttt
     3721 tctttctccg aaaattctga aaaaatcgta ttgacaaaat agttcactac ttttatatag
     3781 ctgctgtact tcaattttcc taagagattt ttcaagatta agaagtcttt tttatcgata
     3841 tttatcaact gtacataaga gtctccagct ttccattcaa atggagttcc ttgggattct
     3901 ctcatgagat gcacaacaaa atacataata aagaaagatt gctcatatgg gctttcttcc
     3961 tgaaacaaat tcatctgaca attattcttg accataattt ctttgatctt ctttttttcg
     4021 attggaaatg gcaacgtgcg tcttccgtag taacttaaaa agaaaaaggt aacaaagata
     4081 cgcaactgga gctcactttt aaggctagtt aactgaaaac cttggtcaac gatcactttt
     4141 aataaatagc tttctaaaat ttgattgcat tgtttcaact tacgtgtaaa agaagaccgg
     4201 ctgatggaaa ttttttcaca ataatactca atcgttttac ccggattgac aataatctca
     4261 tttaataaac gactgtttgt agaactttgc gacatttcca tcagaacatt taaataagct
     4321 ggtacattca aatgttcaag acgatacccc aatttttgtg aagattgaat cgttacatcc
     4381 gttccccagc ggattttgat ctcttcgata taccttacaa atgtagattc tgaactacca
     4441 attcgtttgg caagttcttt tttagtcatc cacttgtcag aaatggacag aattcgaatg
     4501 attttttcca tctttctata gtttcgttcc aacaacctga tcatatgttt ttctccctca
     4561 acccttttct ttcaatgaaa cgctcattaa tcgaaggaat caacttcata aatgaaacca
     4621 attggtatga aaattggtgt gattcccttt agcgcccttc gtataataat tatcatatta
     4681 aaataaataa aattatgtaa cgcctgttta ttttaataat tttcactatt cacgtcaaaa
     4741 cagaaaaaaa gaaaaaggag gggaaaaatg acgaccacag ggaagaaact gaaagttatt
     4801 ttcatgctga taatattgag tttatcaaac tttgtgccat tatctgcaat agcagacact
     4861 acagatgatc caacagtttt agaaacaatt tcagctgaag tcatttcgga tcagtctgga
     4921 aaaaaagcac tgaacatcaa gctaaatgcg aataacacca gtgctgaaaa gatagaaaaa
     4981 gaaattggtc tagtcgaaaa ttacttaagt gatgtggaaa gaaaagaagg agatggctat
     5041 gcttatcagg taaatagcgg gaaaattacg ttggaaatct catcaaacac taaacaaact
     5101 atcgatctga gttttccaat cgatccagca ctttaccaca gccaggcaaa caagctgatc
     5161 gtcgataata aagaatatga cattattgat gagacagaaa ataagaaaga tacagatgtg
     5221 tcagtaccaa agccagacga aatagaagaa gaatcatcaa aagaaaacga aaattctgtc
     5281 agcccattta cattgcctac attatccttg ccagctgtga gtgtgccatc taatcaaacg
     5341 attcctacag aatatacaac agatgatcag ggcacttatc ctaaagccag ttggcaacct
     5401 acaggaaata caaatgttct tgatcatcaa ggcaataaaa acggaacaaa tcaatgggat
     5461 ggtataaatt cttggaatgg agatcctaat gatcggaccc attcgtatat cgaatatgga
     5521 ggaaccggta atcaagcaga ctatgcgata cgaaagtatg caaaggaaac aagtacaccc
     5581 ggattgtttg atgtttattt gaatgctcgt ggaaatgtac aaaaagatat cacgcctctt
     5641 gatctcgtat tggtcgtaga ctggtcagga agtatgaacg acaataatcg gatcggtgaa
     5701 gtaaagattg gtgtcgatcg ttttgtcgat actttagcag atagcggtat cacagacaaa
     5761 atcaatatgg gatatgtcgg ctactcaagc gaaggatata gctacagtaa cggtgcagta
     5821 cagatgggtt catttgattc agtgaaaaat caagtaaaat ccattacacc ttcacggaca
     5881 aatggtggta cttttacaca aaaagcacta agagatgcag gaagcatgct atccgttcca
     5941 aatggacata aaaaagtgat cgttttgctg acggatggtg taccaacatt ttcctataaa
     6001 gtacagcggg tacacgcaca atcaagcagc aattattacg gaactcagtt ttctaatacg
     6061 caagatcggc cgggaaatac ttctctaatc tcaagaatct atgatgcacc tgaccaaaac
     6121 aatctatcca gaagaatcga cagtacgttt atcgcaacca tcggagaagc gatggcactc
     6181 aaagaacgag gaatcgaaat acatggtctt ggcatccaac ttcaaagcga tccggcagct
     6241 ggtctctcaa aagcagaagt agagtctcgt atgcgacaaa tggtttcatc agatgaaaaa
     6301 ggcgatcttt actatgaatc agctgatcat gcaacagata tctctgaata cctagccaaa
     6361 aaagctgtac agatctcagc aactgtaagc aatggacaaa taaatgatcc aatcgcagaa
     6421 ccattcattt atcagcctgg tacactttca gtcaagagtg tggggacaag tcctacaacg
     6481 gtcactccat ctatttccat agaaggaaat accatcaaga gcaatcagat ctatttagga
     6541 aaagaccaag aaatccaaat ccattaccaa gtgagaatcc aaacagaaaa tgaggacttc
     6601 catccaaatt tctggtatca aatgaacggc aggacaactt tccagccaaa cattgatacc
     6661 aatgaattag ctgaattcgg tataccatct gctaaagctc ccggagtcag tcttcacatc
     6721 aaaaagttat gggaagaatt tgacaacaat ctagctgatc gtccagatca agttactttt
     6781 gagattcaac gggaacatac gacaaatgct gcagcttgga aaaacggata tattcgaatc
     6841 attaaaccag ctaaagatac aacaaatacg tgggaacgtg cagacattga caaattatct
     6901 gcaaatagcg gagaaagtta tcaagagata ttatcactac ctcaatacaa taatcaaggt
     6961 caagcattca gttaccaaac aatcaaagaa ttacctgtac caggatacga ttctcaacaa
     7021 atagatgcaa tgacatggaa aaatactaaa caattcacac cgttaaactt gaaaataacg
     7081 aaaaattcct ctacaggtga aaaggatctt attggcgctg ttttcaaatt aacaggagat
     7141 tctattgata ctttactaac agatcatggc gacggaacct attctcttcc agaaaatgtc
     7201 aaattgcaaa aagaaatgac ctatacgctg acagaaacaa aagctccaga agggcatgga
     7261 ttaagcaaaa agactacttg ggaaatcaag atcgcttctg atggtacggt aaccattgat
     7321 ggaaaaacag tcactacttc cgatgatacg atccagttga ctattgaaaa tccttttgtt
     7381 gaagttcctg tagcagtacg taagtatgcg atgcaaggga cggacaaaga gataaatctt
     7441 aaaggagcag cattttccct acagaaaaaa gaagcaaatg gtacttatca gccaattgac
     7501 agccaaacaa cgaatgaaaa aggtcttgcc agttttgatt cactcacacc tggtaaatat
     7561 cgagtcgttg aaacagctgg tcctgccgga tatgatactt cgccgggaaa ttatgaattc
     7621 caaatcgata aatatggaaa aatcatttac acgggaaaaa ataccgagat gacaaataat
     7681 gtatggacgc tcactcatca aaatcgacta aaagcgtttg atctaacggt acacaaaaaa
     7741 gaagacaacg gacagacatt aaaaggagca aaattcagac tgcagggacc agaaatggac
     7801 ttagaatcgc caaaagatgg acaagaaaca gatacctttc tattcgaaaa tttaaaacct
     7861 ggaacttata cgctgaccga aacttttaca ccagaaggat accaaggtct aaaagagcca
     7921 gttactatag ttatacacga agatgggtca attcaagtgg atggacaaga tcatgaatct
     7981 gttctgtcac caggagccaa aaacaaccag atttctttag acatcacgaa tcaggcaaaa
     8041 gtaccattac ctgaaacggg aggaattggc cgtttaggaa tctatctagt agggatgatt
     8101 ggttgtgcgt tttctatttg gtatcttttt ttgaaaaaag aaagaggggg cagctaaata
     8161 tgaaaaaact tggttggctt agtatgtgtc tcttcttgtt actatttaaa ccagctttta
     8221 ctcaggtagc aacagaaaca gaaacagaaa tggttcagat tactttacac aaattgcttt
     8281 tcccaaacgg gcaactgccg aaaaatcatc caaatgacgg acaagaaaaa gctttattac
     8341 aaacgtatcg aggattaaat ggtgtcacat tccaagttta tgatgtcaca gattcttttt
     8401 accatctacg ggaaaagggc aaaacggtag aagaagcaca agcagagatc gcaaaaaacg
     8461 gtgcgtcttc cggtatgttt accgcagaag caacaactac aactcttaac aacgaagatg
     8521 gtatcgcttc tttttctctg gccgctaaag atcaagaaaa aagagataaa gcgtatcttt
     8581 tcattgaatc caaagtacca gaagtcgtca aagaaaaggc agagaatatg gtagttgttc
     8641 ttcctgtaca tggacaaaac aatcaaaaac tttcaactat ccatttgtat cctaaaaatg
     8701 aagaaaacga ctaccctgat ccaccttttg agaaggtatt agaagagctt agaaatgatt
     8761 ttacgattgg tgaaaaaatc acttattcct tgcatacgac aattcctgta aatatccttg
     8821 actatcaaaa gttcgaattg tcagatagtg cggatgaagc attaacgttt ttacctaata
     8881 gtttaacgat ttcatcgaat ggagaaaagc tgacagaagg ctttgtcata cacaagaaac
     8941 ctcacggatt tgatgtttta ttttcgatcc cttcgttgga aaaatatgct ggaaaaaaac
     9001 tgaccatttc ttatcagatg cagctaagca gtacagcaca ggcgaacaag gaaatcaaca
     9061 acaacggaac actggatttt ggttttggtg tcagtacaaa gaaagtctct gtatatacag
     9121 ggagtaagca atttgtcaaa atcgagacaa ataaaccaga taaacgatta gctggcgcag
     9181 tattccttat taaaaacaaa gcaggaaatt acctccagca aacagccaac ggatacaagt
     9241 ggacaaagaa cgaatcagat gcgcttcacc tgatttccga taaaaatggc gctttttcaa
     9301 tttccgggtt gaaaacagga agttatcgat taaaagagat cgaagcacct tctggttata
     9361 ttttaagtga aacagaaatt ccgtttacca tttcaacttt tctttctgag gataaagagg
     9421 cggacagtat attgaaagta gtcaataaaa aagaaaatag ccgtccattt cttccaaaaa
     9481 caaacgaaac gaaaaataca cttttaggcg ttgttggtat ggtattcgca agctttgcaa
     9541 tctggttgtt tatcaaaaaa agaacaggag tgaaaaaatg aaaaatcata aaaaaataaa
     9601 cgttatgtta ggagtccttt tccttatttt accattactc acaaacagct tcggcgcaaa
     9661 aaaagtgttt gcagaggaga cagcagctca agtcatcctt cataaaaaga aaatgactga
     9721 tttacccgat cctttaatcc aaaacagcgg gaaagaaatg agcgaattcg atcaatacca
     9781 aggattagcc gatatttcat tttcagttta taacgtcact caagaatttt atgcgcaacg
     9841 agataaagga gcgtccgtgg atgcagcaaa acaagcagtc cagtctttga ctcctggtac
     9901 accagttgct tcaggaacga cagatgctga tggaaatgtc actttatctt tacctaaaaa
     9961 acaaaatggg aaagatgcag tctacacgat caaagaagaa ccaaaagacg gagtgtcagc
    10021 tgccgcaaac atggttttag ctttccctgt atatgagatg atcaaacaag cagatggctc
    10081 ttataaatac gggacagaag aactagatac tatccatctc taccctaaaa atacagtcgg
    10141 taatgatgga acgttgaaag ttacaaaaat cggtactgcc gaaaacgaag cactaaatgg
    10201 agcagaattt attatttcta aagaagaagg aacaccaagc gtcaaaaaat acatccaaag
    10261 tgtcacagat ggattgtaca cttggacaac tgatcaaacc aaagccaaac atttcattac
    10321 tggtcattct tatgacatcg gcaacaatga ctttgccgag gcatctattg aaaaaggcca
    10381 gttgatcgtt aatcatttag aagttggaaa atataattta gaagaagtaa aagctcctga
    10441 taatgcggaa atgattgaaa agcaaacaat cacgcctttt gagatcctgg caaatagcca
    10501 aacaccagta gaaaagacca tcaaaaatga tacgtctaaa gttgataaaa caacacctca
    10561 attgaatgga aaagatgtcg caatcggtga aaaaattcaa tatgagattt ctgtcaatat
    10621 cccattaggt atcgctgata aagaaggaac gcaaaacaag tacacaacat tcaaacttat
    10681 cgatactcat gacgctgctt taacatttga taatgattct tcaggaacgt atgcttatgc
    10741 cttatatgat ggaaataaag aaatcgaccc agtaaattat tctgtcactg agcaaacaga
    10801 cggattcacg gtttcagttg atccgaatta tattccttca ttaactcctg gcggtacatt
    10861 gaaattcgtt tactatatgc atttgaacga aaaagcagat ccaaccaaag gattttctaa
    10921 ccaagcaaat gtcgataacg ggcatacaaa tgatcaaaca ccaccgtcag tcgatgtcgt
    10981 tactgggggc aaacgatttg ttaaagtaga tggtgacgtt acatcagacc aaacacttgc
    11041 tggagcagaa ttcgtcgttc gtgatcaaga tagtgacaca gcgaaatatt tatcgatcga
    11101 cccatccaca aaagccgtca gctgggtatc ggcgaaagaa tcagcaacgg tttttacaac
    11161 cacaagtaac ggtttaatcg atgtgacagg tctaaaatat ggcacgtact atctggaaga
    11221 aacgaaagcg ccagaaaaat atgttccatt aacaaaccgt gtagcattta ctatcgatga
    11281 acaatcttat gtaacagcag gacagttgat ttctcctgaa aaaataccaa ataaacacaa
    11341 aggtacactt ccttcaacag gcggtaaggg aatctatgtg tatatcggtg caggagtagt
    11401 ccttctactg attgctggac tgtactttgc tagacgcaag cacagtcaga tttagtcttt
    11461 ttgcaagaat tggaaaaagg agcagcggat tacgcaaatg ctcctttttc ttttattatc
    11521 aaacagtcat cacaaggaga acacaaatga aaccaaagaa aaggaaactt ctagatctgc
    11581 tgatgctttt cttgcttctc tcaggaatag ggatactagc ttatccattt gttagcgacg
    11641 cgctgaataa ttatctggat cagcagatca tctctcatta tcagcaacaa gcggtcaaag
    11701 aaaatgaaga agtcatggct aaaatccaag aaaacatgac aaagaaaaac cagcaattag
    11761 caaaagaagg tggaaatcca ggtgcagatc cctttactaa gaaaaaagaa cctgcgaaaa
    11821 aggatagaac ctattttgag aaacatacga tcggtatttt gacgatccct gagatcaatg
    11881 tccatttgcc aatttttgat gaaacgaata ctgtgctgct agaaaaaggt gcctcattat
    11941 tggaaggaac gtcttatcct acggggggag aaaatacaca tgccgttatt tccagccatc
    12001 gaggactgcc tcaagcaaag ttgttcacag acttaccgaa actgacttta ggtgattctt
    12061 tctatatcga aatcaatgga cggacactcg cttatcaagt cgatcagatc aagacggtag
    12121 aaccgactga tacaaaagat ctgagaatcg aaaaagggca ggatttcgtg acactcctaa
    12181 cttgtacacc ttatatggtc aacagtcatc gcttacttgt cagagggcac cgggttcctt
    12241 atcatgcaaa agaagttcaa aaagaagtac agaaagtatc cgagaacaaa cgatttttcc
    12301 tgtatggatt gttagctgca tgtatactga ttttcctcct tcttatatat ctttttcgtc
    12361 aatcgttcaa aataagaaac agaagttagc tattcctcat ttctataata tatctattcg
    12421 aatcgttccg taaacaaaca ggctgaggtt tgaaaaagaa aacttcagcc tgtttttatg
    12481 atgatatcaa agagagccta tcaaacacta tctttcttat tactaaatga ggatcttttc
    12541 ttaagttttc cgatattctt taagaaggcc tttttgcatc aattcttgtt tgtttttcaa
    12601 gttgcggata ttctcttcaa aattttgaga atatatcact gagaataacg tatttaatac
    12661 gtaatcgaag gcaatttgag aggagaaagt cgctacttta gcaaaatcat attcctcctg
    12721 cgatataaca agacacatat cagcctgttt ggccatttct gaatgctgat ttccggtaat
    12781 cagcaaggta ggggcctcta catgcttcaa ataggtcatt attcgttcat aatgatggat
    12841 gcgaccgctg tagctaataa aaatggcgca atcctcgcta gtcaaattgg ctgcattcca
    12901 agaagaatca ctgtattctt cagccatgat caaaaactta ttcaacttga ccattttatt
    12961 ctggaatttc cttgccgtga tttgagaatc accttttgca aaaagaaaga ttcgctgtgc
    13021 cttgttcaat actttaccga tttgatctaa atcttcatgt tcaatctgca attgtgcttt
    13081 ttttattgac tggatcgata aatccgccat tttctttgca atttgctgag tgctatcttc
    13141 acttgaaaaa ggaaaattag gatcaatcgt ttgattgaac tgagcaatca attgatgaac
    13201 ttcccgcccc agctcgaaac ggaattcttt aaatccgctg aatcccattt ttttgcataa
    13261 acgaatgata gctgaatggg aagtatacgt agcttttgct aattcttcaa tcgtcatgta
    13321 gactgcagct tcgatattcc tacggatgta atcgacaatc cgtttctctg tcgtggtaaa
    13381 caactcttgt tttgttagtt tttcttcaat aagcatgatc tgacctccaa attctatctt
    13441 actgataaac acttggttcc gctactatct ttttgatttt tatccttact ttgaacatta
    13501 atttcaccaa accgaatgat aacaagtgtt tttctgaact tttttaccag aatagctatt
    13561 ataaatactt tctgcgcttt tctaggatat actgaaaaca tcaataaaag gaggcaccac
    13621 tcatgcttac aatcgcttat cttggcaatg gaaagagtac aaatcgctat catttgcctt
    13681 tttcaagaaa acttacgaac aaaatcaata tcaaaaaaat tttttcccca tctgaaccag
    13741 tgtggaaaac attcgaagac atcgaatata ctgatcagct gtctgatctt tgggatgatc
    13801 ctgagatcca attagttgtc atcactacac ctagtcaatt tcattatgaa tatggaaaaa
    13861 tggcactcga acatggtaag aatgtactag tagagaaacc ttttgcagaa acgtcaaagg
    13921 aagcagaaga gttatttgca ttagcaaaag aaaaaggtct atttatccaa tgttaccaaa
    13981 acagacggtt cgattctgac ttcttgacag tccaaaaagt aatcgaaagc ggaaaattag
    14041 gggatatttt agaagtagaa atgcattatg attatttcag accggaagta ccagaaaata
    14101 cgcatgaatt ttctactgcg aacagctatt tatatggaca tgcttgtcat acagtagatc
    14161 aagttctttc ttatttcggc gaaccagatg atatcagcta tgatgtacgt caacttttag
    14221 ggaaaggacg aatgaatgat tacttcgatc tggatttttt ctatggaaat atgaaaattt
    14281 cgataaaatc cagttatttc cgcatcaaag aacgtccaag tttcgtcgtt tacggaaaaa
    14341 aagggatgtt cgtgaaacaa acaaaagatc gacaagaaga acatctgaaa atgttttaca
    14401 tgcctgatca tgaagacttt ggtatcgatt tgcccgaaca ttatggtgta ttgacctata
    14461 tagatgaaca agggagttat catgaagaaa aagtaatttc cgaggttggt gattacagcc
    14521 gtgtctacga agggatttac gagacattag ttaatggcgc agaaaaagtc atcaaagatg
    14581 aagaaacaat cttacaaatg aaaattttgg aagaaggaat caaaagcatt caagaatcag
    14641 aaggaaaaat atgacatatt tgtaagaatc gggtttgttt tttgacgcat aaactgtctt
    14701 attgtacgat gaaaaagtat agaactacta ggaggaacca acatgacaaa ttatgacggt
    14761 gaaaaacaca aacgattaag aggttcaatg gcccctaaaa cagaagaaga agtggcttta
    14821 gcaggaacta attctggtga aacagaagac aaagaagaat cattagctgg cacaaactct
    14881 ggtgaaacag aaaataaaga aacagcttta tctggttcaa attctgtaga agaggaaaca
    14941 aagcctaatg agcccaatca ctttgaaaaa gatatcgaaa aagcggttga agatgtcaaa
    15001 gaaaaatttg aagaactgac ctcaaaaaat aaacatgatc atcattgatc ggacgaaatc
    15061 aattcataaa agcaggagac atttggtcac ctgctttttt tctgtatcca ttcgatagaa
    15121 atcgttcggt acagtcgtaa aaaaaatcca actgtaagtt gtaccaaaca cccctcgatt
    15181 taaaagcgct accatactag aatataagta ggaggtgaac agcgctgtga actacaaaat
    15241 tagttatctc ctgaaaacac caagagaaat cagtctagaa aaagcagcag agatcatcga
    15301 agtaaataca agcgaggcga tcaaacaatt aactcctttt gccgaatatg tggatgatac
    15361 ccgtatccgt ataaagaaat tccgagcaat cgactgggca gaaaaatttt tggatattac
    15421 tcgatttgaa gctgtgtata ccgaacaaga gcgaagagat cttctttatt tgatggttta
    15481 ttcgactgca acgtctgttt ctgtttttca ttttcaagat tttcttcagg tttctaaagg
    15541 gacagtcctg tcagatatca aaaagataag aaatgaatta gaagtggaag attgtcagct
    15601 gtcgtatact aggaaaactg gttttaggct gctagggtca gaagcaatat taagaagaga
    15661 agcaaaaaat cgcactgctc gtttgatgca gtcacttgct ggaagatttg ggctcacgta
    15721 tttaaccgca accctttcca ttgattatta cgccaaagtt agagatttgc tagataaaac
    15781 gatttcacta tcagatcttc gattcgcacc aaatcgaatg gatgaattag cttattattt
    15841 gagtttgaca accattcgaa ttggaaaaac atccatggaa ctggtaaaag atgctgaact
    15901 gttggcttct cttgcagtca ctgacattag tcgacaagta ttgactggcc ttggctattc
    15961 cgaacatcaa aattcggaga ttatattttt ctctctttgc ttagcatcga tcgttgaagg
    16021 agatgtccag gagcctgcac ttgatttttt gctggaatgt tcttcagaag tcatccatcg
    16081 catggaattc ctgatggccg tagaatttgc ttcgtttcgt gaattattga tgaatgtctt
    16141 ttatcacttg gtccctgcct attttcgtat ttcctattct ttttatttac ctaatgtgat
    16201 gatcgatcaa attaagcatc aatatgcttc catctacgaa atgacaagaa aagcattact
    16261 tccgttagaa agacgaatag ggaagagcat ccctgaagaa gagattggtt ttttcactat
    16321 actatttggc ggagagatca gaaaagcgga cgcagaagaa agaaatagaa aaataagagc
    16381 cgtgatcgtc tgtccaagtg gcattagctc ttcattgatc ttaaaaagtg aacttcagca
    16441 attattcccg atgatctttt ttacagagac taattcagct gaccgcttac acgaggttga
    16501 cgaaacttct tacgatatta ttttttctac tgtacctctc acacttactg ataagaaaaa
    16561 gtctctctat gttctgcatc caatcatgac tgacttggaa aaaaatcagc tgatcaatca
    16621 agtacaaaga gattggctga taccaggatt ttcaatgcct tctgcccaag aattgttgtc
    16681 ggcgctactg ccttatatcg aattaaaaga aggcgtagac gaagaaaagc tctatcaagt
    16741 attaaatcgt aaaatgaaca aacttttaga aaagaaggag gatcaacgtc ctatgctatc
    16801 tgaattatta acagaagaaa tgatccaatt atcggatcaa ccaaaaaact ggcaagaagc
    16861 aatcacattt gccgcacagc cgctattatc agaaaataaa atagaagaaa gttatatcaa
    16921 agctatgatc gagcgcgtgg agcagtatgg tgcgtttatc catattggcg atcacatcgc
    16981 attacctcat gctcgtccag aagatggcgt aaacgaagta gggatgtctt tgctgaaact
    17041 tcaacaacct gtggatctgc ttgataatcc agcccatccc attagtttgt tcatctgttt
    17101 agctgctatc gataatgagg cacatcttcg ggctttggca aatttaacaa agctactttc
    17161 taataaacaa aatcttcaag atttattaca cgcatcaaca aaagaagaaa tattagcgat
    17221 cataagaaaa ggagacgaat gatatgaaaa tttttgcagt atgtcaaagt ggattaggaa
    17281 caagttttat ggtagagatg aacatcaaac aagcacttgc agatcttgga gcagatgtgg
    17341 acgagtttga agtaagtcat acggatgttg gaagtgtatc tagcgatatg gctgattatt
    17401 tcttcattga gcattctttg aaagatacgc taacttctat acctgatgaa aagctcgtac
    17461 ctttgcaatc gatcattgac agcgaagaag taaaagaaaa agtaacgaag atcttgtcaa
    17521 aagaataaaa atgattcaag ggaggaacta tcaatggata aattgttgaa tttgtttgtg
    17581 tcaatcgtca gtcagccctc catcctcgtt tctttgattg cactgcttgg tctcgttctt
    17641 cagaagaaga aattttcaga agtgatccaa ggaactatca aaacatttgt cggattccta
    17701 gtactgatcg gcggtgctgg tttaatcagt aattcactaa ccccttttgc ttcgatgttc
    17761 caaacagctc tgcatactca aggagtagtt cctagcaatg aagcagtagt ggcgattgct
    17821 ttgcaacaat acggtacacc tactgctttg atcatgctag tagggatgat cgtgaatatt
    17881 ttacttgcta gattcacaag atataaattc atttttctta caggtcaagc aatgctttat
    17941 gtttcttgtc taacagcagt cattttagta agcgcggggt ttactgcaaa tttgtggatg
    18001 atcttattag gcggggtctt cgaaggaaca ctattaacta ttacgcctgc tctttgtcaa
    18061 ccgtttatga agaacatcac tggaaacaat caagttgcgt tagggcatac cggaaatatc
    18121 ggttatgctt ctagtggatg gatcggaaag ttttttggaa acaaagaccg ttctacagag
    18181 gacataaaga ttcctaaaag tttcagcttt ttgcgagatt ctactgtatc gatcatgctc
    18241 ttgatgtcga tcgtatatgt cgcacttgct ttgttagctg gtcctggatt tgttgaaaga
    18301 gaactaagca atggagacaa cgctgtaatc tttgcactga tccaagctgg aacattcaca
    18361 gctgggtttg ttatcgtcct tcaaggggtt cgaatggtgt tggcagaaat cgttccagca
    18421 ttccaaggga ttgctaaaaa actcgtaccc gattctaaac cagcattaga tgttcctatc
    18481 gtttttccat atgcacctaa tgctgtatta attggctttt ttgttagctt ctttgtagga
    18541 acactatcca tgtttgctat gataggcttg caaactacag ttatcattcc tggggtagta
    18601 ggacatttct tctgcggagc tgcttctggt gtcttcggca actcgactgg cggtagaaga
    18661 ggggcggtat taggttctgc tttcaatagc ttattgatta gttggctgcc attatttatc
    18721 cttccagtat taggagatct taaaatcgct tcttctacat ttgccgatac tgattatcta
    18781 gtaccaggac ttgtacttgg gaaactagga gaatacggtc aaatagcctt gacagttgga
    18841 atcatcgctt tctttttact tgtcgtatta tctagctttc tttttgccgg aaaaagaaca
    18901 aacattgttt ctaaagaaca gaattaagca aacttcaaaa taaaaattgc ttggacaata
    18961 tcagaagaag atttaaagtc ttctgatatg tcgcaagcag ttttttgttt tatgcattta
    19021 tttttgaata gcttgttaag acaagtggct gttttgctag aagaggataa cttttaccat
    19081 tcattttcac tgttccgcct gttctatcag aagtaatcac gatcttatct tttgtttttt
    19141 caagcgaaat ctttccatct ttgataggta gcgtacaatt cagtgtttca aatgaatcta
    19201 gattaggttg tatcataaat tcttcataac ctggctttgt tggatacaaa ccgataaaat
    19261 agcgtcctaa aagatagatt gggcttgctc cccatgcatg gcataagctt tttccataag
    19321 gatctccata catactgtac ttttcatttc ctgtttgagt agggtcgtat tcttcccaga
    19381 aagttaccgc atcatgagat aacatccctc cccaataatc aagaatcgtt tggtagacga
    19441 tattcgtctg tccgagtttg cataaagcat cttgttcata gaatttgaaa tatggagtag
    19501 taatctgtgt gatagtgtca ttcaataaga cattctttaa gatcgattgc tgttggtctt
    19561 tgttagcaaa atcgaataaa acagcgaaaa tattcgcatg acgcgtcaca tttcttctcc
    19621 cagattcata gcaatcgata aaagcgccaa gttcttcatc ccaaaagtag tcaaataggt
    19681 tttgtttcaa tagttgatat ttttcttgat agagatgagt atgataattt tcgttctctc
    19741 catatgtttc agctaagata tctccgcaag tgatcatgga atgataggct ttcagtaata
    19801 agacttgttc tgcagcaagc gttccttctt tatccatttc agaccaatca ataaatatcc
    19861 aatcttttgc tctcccataa ataaacccta attcattcgt ttgctccatc aagtatctca
    19921 ttaatgattg catcttaggg tagacgatgc gtaaaaattc atagtctttc gtcatcatat
    19981 agtggttttc gataccaatc aaccacaaga tcgaatagtc aacgatagta ttcatatgct
    20041 gcttgatttc attttgtcct cttaatgcta atatcgttcg tttattgatt tcctcgtcaa
    20101 aaaaaagata ttgattgatc atgaatgatt gatacgcatc tccagaccaa atccagcgat
    20161 ctcgtttgat accgtcgata aaaaacaaat cactacataa cttgaaggct tcttctgata
    20221 ctgaccagat ctgattcagt aaagaagaat cgcttgaaaa agcagcttgt ttcgaaagag
    20281 ataaaaattc atgaaaaact tgaagttgaa ccattccagt agaaacctct ggtataaaca
    20341 aatagcgaaa agcgcgttta cgagctttca tttttcttgt cattcctatt tgcttatagt
    20401 agcaataatc gatatccata gcttctacat cggactctcc ataacataga gtgatagatt
    20461 cgatatggtc atccagagat attagttcta cacagccatt cactgctcgc ccaaaatcga
    20521 ataagattcc tccgccaatc tccgtttcag tgactggatg gacgcattct ttttgataat
    20581 agatttgatt tggatctttt gcgatatcga cataaagcgg actccagcca gctggatatt
    20641 cttcaataaa attacttgct gtccagccaa tatctgattt tacttcgtct ccctcgataa
    20701 acatcgctgg tagccctgat gtatgtccta caaagatacg aatttttgct ttaccagctg
    20761 gacaagtcag ccattttcca catggatgtt tttgtccatt aatttctaca tacccaactc
    20821 cttggatcgt cactttaaac atggaaggtt gatcaagaaa gtaatatctc ttaaacttga
    20881 cgttccgatg gcaatcatcc atgtaccagt acgctggcca gtcaaaacct cgttcttccc
    20941 gttggaaatt ttgtagcagt ccatggcgga tctcaaagtc tccaggatac caaagccatc
    21001 tatctttatg tttttcattt gtcattttac acgacctcca tcaaatttat tttgttgtca
    21061 attgttcttt tatttcttca tctactctta tcactgtctt tctattcgtc ctgtgtacta
    21121 ctagaaggct aataagcgcg ctaatcaaca gcaagatact gccattgacg aaaacagcaa
    21181 gtgttttact cccaagagaa gcaaaagaga gaaagagcaa accatatggt gcaatgaaag
    21241 tacctaaatt agcgccaacc aataagattg aagtattgaa actctccgcg tacttgggcg
    21301 cttggatact tacttggttg aataagtaag gaatgaataa cgaaaaagaa attcctgtta
    21361 ggatcgcacc aaacccaatc acatacaggt ttatcacagt ggcaatcaat acaaagccga
    21421 ttcccatcag tagtaaagaa agtgtaagca tatagcgatg tgcttttgtc tgaagccagc
    21481 caaaacaaac tccggcagcc atacttgcca attgcatgat tgttaagata ttcgctgcat
    21541 tagtttcgtt tcctagagaa taagtcgtca ggactgtggc taatttaatc gatacagcat
    21601 tatataccac gatgaccagc aagagaaaaa taccataact aatcatttta ggattcgttt
    21661 tgattacttg tcttgttggg gctgtgccat gcgattgaag ttcagttgtt tcaggtgtag
    21721 gaacaaacaa aacaaacaag acaagaatag gtaagctgat ggcgtagact aaaaaggtat
    21781 attgccaacc gaactgtact agctgtcctg caacaaatgt cattaaagct gcaccaaatc
    21841 cttgaaatgc actttgaaaa ccaatcatag aggctcgttc atctccgtga taaaaggcag
    21901 caatcatgct gactgccaaa gaattgatca atccaaagcc aatacctagt ccagctcttg
    21961 aagctaagac aatactgtaa ctttgtgaga agagggggag gataccacaa atcgatgcaa
    22021 tcactaatcc gatgatcaca gtttgttttt gtccgatttt cctagcaata aaactactaa
    22081 tcaaaacaaa aatagtgacc ataaaagagg gagcagtcgt taataattca acagcagata
    22141 aagaacgcgt ggcataagta gctgacatac tgggaattgc agacgtgact gcaccagcag
    22201 aagtcaaaat aagagagact gacagcagag aaaatttcat catcttttta ttcataagca
    22261 tgttcctttc atttttgtaa gcgcttatca aaaatatagt atgaagtggt tattctctca
    22321 atagcggaat cacaggaagt tcatgtccca accatagaaa gtctttatga aaccaaaaat
    22381 agctttgcat ataatagcag tacagcgtga actaataagc gaaagaaacc gcttacaaaa
    22441 aggagggagt caattggaac tagctgaatt agacattttt tttgcgtaac gaagaatcaa
    22501 tcgaaactct gcagcgtgaa caaggaatga atatcaatga tttaaactta gcttaccatc
    22561 ttcagacacc gccaaaaatc caccgagggg atctatttca acagggaaat attcatatca
    22621 gtaaacacct caaagaatcg aatacacagg gcaagaggat attttaatca acatcttatt
    22681 aaaagaagaa accttatcta cagagatatt gaatcatttg gcaagcacaa ataatctact
    22741 ttcttctttt ttgataaatg catcaaaaga aaaaggggaa catacacaat tcattcattt
    22801 tcgcggcgga aaaacaagtc gtttgacgca tttgattgaa aatatgctgt tgatttattt
    22861 tgatacatac agtcataaga tccgttcgtt aaatctctat acatcactat tactgatcga
    22921 attgactcat ttattagaac aacaaagctt ttcatcagca gatggatgcg cagaaaatga
    22981 gatgcttaga gtattagatt atatcgataa gcattttact tcttgtacgt taaaagaaac
    23041 agcagctcat tttggctaca atccggtcta tcttagtaat aaattgaaaa tgtattttga
    23101 tgcttccttt caagaactgg ttctaaagaa aaaattcgac tttgcgttgg aactgattca
    23161 agaaacgagt ttttcgttgg aagaaatcgc taatcgtatc ggatataaac aaacgacttc
    23221 tttattcaaa ttattcaaga agtattcaac atgtactccc aatgaatgga gaaataaaca
    23281 ttaagaagaa aatctttcac taaagcatat gaaggaaaat tgttttaggt gcgattgctg
    23341 tcttattata tcgttgcagc gaattttttt ggatttattt tcagttctaa acttttggtt
    23401 gtattatcag gggatatagg tgtaaacatt tgtcatttat ctgttatgcg tattcaggaa
    23461 ttcgtttcca tcaaaaatta taataacaac tattataatt aacatgttga taaaatgaat
    23521 aaacaagact atacttttca tgaaagtaac aaatagttat gaaaaggagc tgtttattca
    23581 tgaaaaaaac aagtcgtaaa gtagtaatcg tcggtacagg ttttgttgga acaagtatcg
    23641 cttatgcaat gatcaaccaa ggaatctcta atgaattagt attgatcgat gtgaatcaag
    23701 aaaaagcaga aggggaagca ttggaccttt tagatggtat ggcttggggc gatgaaaatg
    23761 tggctgtatg gtcaggtggc tatgaagaat gtaaagatgc tgatatcgtt gttatcacag
    23821 ctggtatcaa ccaaaaacct ggtcaatctc gtttagattt agttaaaaca aatgcgtcaa
    23881 tcatgcgcca aatcgtaaaa gaaatcatgg gatcaggatt cgacggtatt atcgttgttg
    23941 cttcaaaccc agtagatatt ttgacatata tcgcttggaa cgaatctggt ctgccaacat
    24001 cacgtgttat cggtactgga acaacattgg atacaactcg tttccgtaaa gaaatcgcat
    24061 tgaaattaaa agttgaccca cgcagcgtcc acggctatat cttaggtgaa catggtgatt
    24121 cagaagttgc tgcatggtca catacaactg tcggtggtaa accagtgttt gaaattgtag
    24181 aaaaagacca tcgtattgcc caagatgaat tagatgtgat tgctgataaa gtccgtaatg
    24241 ctgcttatga gatcattgat cgtaaaaaag caacttacta tggtattggt atgagtactg
    24301 cacgtatcgt aaaagctatc ttgaataacg aacaagctgt attgcctgta tctgcttatt
    24361 taactggtga atacgacgaa aaagatatct ttacaggtgt gccatcaatc gttgacgaaa
    24421 atggtgttcg tgaagttgtt gaattatcaa tcaacgaaga agaaaaagca atgttcagca
    24481 aatcaacaag tgctttaaga gaagtattga acactgtatt ataagaaaca atgaatatga
    24541 actaaaaatc ctaaaactgc ttaacagctg aaatagctgt taagcagttt tttattgata
    24601 tctaataggt aagcaaataa acagaatggt taaaattaat cggtccgaaa atgtatccgt
    24661 acatatgaac atatgaaatt taatctgttg attacaataa gcgaatcgtt atcaatgttt
    24721 atctgtggtt aattaaaaaa tccttccgca acggcaaata caatgattca tatcta
//
LOCUS       DAHHPA010000038        24455 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.039, whole genome shotgun sequence.
ACCESSION   DAHHPA010000038 DAHHPA010000000
VERSION     DAHHPA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.039"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(53..787)
                     /locus_tag="NBH55_001374"
     CDS             complement(53..787)
                     /locus_tag="NBH55_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCD1509365.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="NBH55_001375"
     CDS             1114..1524
                     /locus_tag="NBH55_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCD1509366.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="NBH55_001376"
     CDS             1517..2218
                     /locus_tag="NBH55_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCD1509367.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="NBH55_001377"
     CDS             2218..3804
                     /locus_tag="NBH55_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1509368.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="NBH55_001378"
     CDS             3823..4044
                     /locus_tag="NBH55_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCD1509369.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="NBH55_001379"
     CDS             4041..4802
                     /locus_tag="NBH55_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCD1509370.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="NBH55_001380"
     CDS             5182..5691
                     /locus_tag="NBH55_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCD1509371.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="NBH55_001381"
     CDS             complement(5766..6305)
                     /locus_tag="NBH55_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCD1509372.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="NBH55_001382"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="NBH55_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCD1509373.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="NBH55_001383"
     CDS             7318..8169
                     /locus_tag="NBH55_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCD1509374.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="NBH55_001384"
     CDS             8197..8832
                     /locus_tag="NBH55_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCD1509375.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="NBH55_001385"
     CDS             8852..9625
                     /locus_tag="NBH55_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1509376.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="NBH55_001386"
     CDS             10033..10830
                     /locus_tag="NBH55_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCD1509377.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="NBH55_001387"
     CDS             10808..11221
                     /locus_tag="NBH55_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCD1509378.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="NBH55_001388"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="NBH55_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCD1509379.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="NBH55_001389"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="NBH55_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCD1509380.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="NBH55_001390"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="NBH55_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCD1509381.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="NBH55_001391"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="NBH55_001391"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCD1509382.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="NBH55_001392"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="NBH55_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCD1509383.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="NBH55_001393"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="NBH55_001393"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCD1509384.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="NBH55_001394"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="NBH55_001394"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCD1509385.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="NBH55_001395"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="NBH55_001395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCD1509386.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="NBH55_001396"
     CDS             21905..22483
                     /locus_tag="NBH55_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1509387.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="NBH55_001397"
     CDS             22535..23017
                     /locus_tag="NBH55_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCD1509388.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="NBH55_001398"
     CDS             23134..23508
                     /locus_tag="NBH55_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509389.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23938..>24455
                     /locus_tag="NBH55_001399"
     CDS             23938..>24455
                     /locus_tag="NBH55_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCD1509390.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQT"
BASE COUNT         8019 a         4398 c         5216 g         6822 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
    23881 gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga cttccctatg
    23941 aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt aaatgaatta
    24001 ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt aacggctttt
    24061 ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag aaacggttct
    24121 tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc tagagatcgt
    24181 aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga tacattggaa
    24241 accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc tgatttgatc
    24301 gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac taaagttctg
    24361 actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt cgctattttt
    24421 atggacgcta cttacattcc actaaaacgt caaac
//
LOCUS       DAHHPA010000039        24117 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.040, whole genome shotgun sequence.
ACCESSION   DAHHPA010000039 DAHHPA010000000
VERSION     DAHHPA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.040"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            42..3569
                     /locus_tag="NBH55_001400"
     CDS             42..3569
                     /locus_tag="NBH55_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCD1509391.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3566..7288
                     /gene="addA"
                     /locus_tag="NBH55_001401"
     CDS             3566..7288
                     /gene="addA"
                     /locus_tag="NBH55_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCD1509392.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7357..7698
                     /locus_tag="NBH55_001402"
     CDS             7357..7698
                     /locus_tag="NBH55_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1509393.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8007..9050
                     /gene="pheS"
                     /locus_tag="NBH55_001403"
     CDS             8007..9050
                     /gene="pheS"
                     /locus_tag="NBH55_001403"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCD1509394.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9055..11475
                     /locus_tag="NBH55_001404"
     CDS             9055..11475
                     /locus_tag="NBH55_001404"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCD1509395.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11645..12655)
                     /gene="trpS"
                     /locus_tag="NBH55_001405"
     CDS             complement(11645..12655)
                     /gene="trpS"
                     /locus_tag="NBH55_001405"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCD1509396.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12909..13646
                     /locus_tag="NBH55_001406"
     CDS             12909..13646
                     /locus_tag="NBH55_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD1509397.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13660..14475
                     /locus_tag="NBH55_001407"
     CDS             13660..14475
                     /locus_tag="NBH55_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCD1509398.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14488..15135
                     /locus_tag="NBH55_001408"
     CDS             14488..15135
                     /locus_tag="NBH55_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD1509399.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15151..15807
                     /locus_tag="NBH55_001409"
     CDS             15151..15807
                     /locus_tag="NBH55_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD1509400.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            15969..16790
                     /gene="racE"
                     /locus_tag="NBH55_001410"
     CDS             15969..16790
                     /gene="racE"
                     /locus_tag="NBH55_001410"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCD1509401.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16793..18148
                     /gene="rph"
                     /locus_tag="NBH55_001411"
     CDS             16793..18148
                     /gene="rph"
                     /locus_tag="NBH55_001411"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCD1509402.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18149..18667
                     /locus_tag="NBH55_001412"
     CDS             18149..18667
                     /locus_tag="NBH55_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD1509403.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18774..19274
                     /locus_tag="NBH55_001413"
     CDS             18774..19274
                     /locus_tag="NBH55_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD1509404.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19554..20474)
                     /locus_tag="NBH55_001414"
     CDS             complement(19554..20474)
                     /locus_tag="NBH55_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCD1509405.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20639..21652
                     /locus_tag="NBH55_001415"
     CDS             20639..21652
                     /locus_tag="NBH55_001415"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCD1509406.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21762..22517)
                     /gene="fabI"
                     /locus_tag="NBH55_001416"
     CDS             complement(21762..22517)
                     /gene="fabI"
                     /locus_tag="NBH55_001416"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCD1509407.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22737..23177
                     /gene="fabZ"
                     /locus_tag="NBH55_001417"
     CDS             22737..23177
                     /gene="fabZ"
                     /locus_tag="NBH55_001417"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCD1509408.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23292..24053)
                     /locus_tag="NBH55_001418"
     CDS             complement(23292..24053)
                     /locus_tag="NBH55_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD1509409.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
BASE COUNT         7971 a         4219 c         5099 g         6828 t
ORIGIN      
        1 taataaagga gcaccgtatc atgtgttgaa ggaggaaaag catgtcttta caatttattc
       61 ttggaaacgg gaaaaaagat caccgtcaag tcttgatcga cgaagcatcc gcttggctcg
      121 accaggatgc aagtaaccaa gtatttttcc ttgttcccaa ctacagcaaa ttcgaacagg
      181 aacaagaaat tttagcggag atgcgaagaa gaagcggaaa aaaaactttc agcacaacaa
      241 acgttcaagt atttagtttt tatcgtctgg cttggtattt gctgcaacag acaactcttc
      301 ttacaggaaa tgaattatca gaatcaggat ctgcaatgat cttgcgacag attttagaaa
      361 aaaatgagga agaattaaca atctttcgcg gagaaatcaa taaaagaggg tttattcgtc
      421 aattacaaga actctacagt gaattgcgta caggaaatat tcaaccagaa gacctgatgc
      481 ttttatttgg tgattcacct aatgcagaag accagcaact taaaatgaaa gacttgaagc
      541 tgattttttc agcttatgac agcgaattga ctcaacgtgc actaacaaat gaagatgcac
      601 tatctatact agcaaattat atgcagacac aagatttcaa caacgtcaaa tttattgtca
      661 gtggtttttc tcgaatcaat ggtcaagagt atcaattact gcaggcaatg atggaaaagg
      721 ggcaattggt tgtcgattta ttgctggatc gtccttatac agcggatctt ccagaagtac
      781 ttacgctctt tcatgagact ggaaagcttt atttccgtct ttttcaaagt gcaagagaac
      841 ggaaagtccc tatattagtg gaccgatttg caccagagca gactcgtccg acagagcttc
      901 aactgctgca aacaatgtgg gaagagacat ctagccaaaa gaaagcggca gtacacaaaa
      961 tagggggaca agagcatctc catttgtggg aagctgaaaa tccaactgaa gaagtccgac
     1021 aattagctgt tgaaatcaga cgactagtca gtgaagaaaa ctaccgttat cgtgatatcc
     1081 aagtgctgac aaaaaatatg tctttatatg gaaatatcct tcggccgatc tttcaagaaa
     1141 tggcaattcc tttttatatc gatgaagaac aaatgatgga aaatcatccg ctgatcgaat
     1201 ggatcaacag tctgttcgct cttgaccgct attcttatcg gctaagtgat attatgcgtt
     1261 ttttcaaaac tgaattattt ctgtcgacgg aagcagaaac cgaacttgat gaatggaaac
     1321 aagagcgaga tgaattcagg aaaaaactgg acatcactga aaatgtagca cttgcttaca
     1381 attatcaagg aacttattgg actcgtgaaa aagactggca gtttgtggcg tatgattttg
     1441 aagcagaaca gattgaagac gtacagtatt tggaagaaca gtcaaatacg attagacgat
     1501 tatttcagaa agctgtacca gtatttttga aaaaaataaa gaaaagcaaa acaggacaag
     1561 aggccgcaaa tgttttttat caatttttga tccaaagtgg cgtacagcaa caattattgt
     1621 tttggcgtaa tcaggaagta gacagaggaa acttagaagc ggcaagaaac catgagcaga
     1681 catggggagc gctgattgat ctgttggatg aatatgtagt aatttatggt gaatcggctt
     1741 ttgactggtc actgttccaa gaaatcatta caagtggtct agaaaatcta agttatggta
     1801 aaatccctac cgcaatcgac caagtgcgaa tcaatcgttt ggaattagtt cgaccaaatc
     1861 aagctgccgt cacttttgca ttagggttga atgatcaagt gtttccagac cgaaaagaga
     1921 caaaaggatt gttgtccagt gaagaacgag aagtattaaa tcaggccttg ccagatggac
     1981 agtttttatt cgacccatca aaggaaagta tctcttttga gcctttccaa gcctatcttg
     2041 tttttctttc cggaacagat cgattatatc ttagttatgc acaaagctat gacacagatg
     2101 gttcgctgaa gatgtctcct tatttgcgta gaatcacaga atatttatct gttcccgtcg
     2161 agagaaaaga gcatttgacg atcgaaagcg accctaattg ttatgtaggg acttacagaa
     2221 gcggaatcaa tacagtcaat aggatttatc gattagcttt agatgaaaaa aaacctgtac
     2281 caaagcactg gcaaaagctc aaagaattag tactgaattc gtcatggcga aattttgctt
     2341 tacagatttt tgaaagtcag gaacatttaa atgttcctgt ttcgatgcca gaaaaaatgg
     2401 cggaagagct ttatggaaaa gagatttata cttcaatttc acgtatggag aatttttata
     2461 attgtgaata caaatacttc atgcaatttg gtttgaaatt gaaagaacga actgtttatg
     2521 gtctaactcc agcagcaact ggtgattttt atcatgaatc attggatcgt ttcttcaagc
     2581 tactgttttc aaaccaatta tccttagtga atatgtccga tgcgcagcgt agagacttta
     2641 ctgaaaaagt gctgcaggaa gtattcggag aacttcggtt tgagatattg gatagttctg
     2701 caagaatgaa ctatatccgc taccagttag gacagacgat tcaaaaagtg acatgggctt
     2761 tgcaaaaaca gagcaaaaat acaggcatgc atcctcttca gacagagatt ctatttggtc
     2821 agattgctgg agcaaaaggt atcacaggtc tggaattacc gttgagtaat ggtggaaaac
     2881 tccatgttcg agggaaaatc gatcgaatcg atgtagcttc tgaacaagaa gatacttggc
     2941 taagcgtcgt tgattacaaa tctagcggac gttcattcga tgtcacagaa gcttattatg
     3001 gcatggctat gcaattattg acgtatctgg acgtagcatt gatggatgcg gtacgtttga
     3061 ctggaaaggc ggctgttaaa ccagcaggat catattattt acatgttcat aatcctgtat
     3121 tgacggagac agatgatgta gagaagaata cattgaaaaa atttagttat gatggcgttt
     3181 ttgtcgatga tcccgctctt ttagaagtac tagatcattc actggaagca aaagaacaat
     3241 cactgatcta tccagtgaaa aaaaatgcga aagatgaata ccaaaaaggt agtttcagta
     3301 aagacaaatt tttcacggaa gaagaactgc aactattcat ggcgcataat cgggaaaata
     3361 tgcggttagc tggagatgcg attacttccg gagaaatcaa actaaatcca gcttataaag
     3421 ataaacagcg gattgcctgc gaattttgtc cattcagaag catctgtacg tttgatgtga
     3481 tgctgaaaga aaataattac caccgtctgg agaaaataga taaggaagaa gcactgcgta
     3541 gaatattgaa aaggagtgaa gaagagtgaa tcaaattcct ttaaaaccgc aaaatgaacg
     3601 attcacagat gatcagtggc aagcgatttt tgatcaagga gataatctat tagtctctgc
     3661 gtctgccgga tcaggtaaaa ctacagtgct tgttcgaaga gtgattgaaa aactaaaaat
     3721 gggttttgat atcgatgaat tattgattgt gacatttact gaagctgccg caagagaaat
     3781 gaaagaaaga attcaagagg cattacaaga atccgtcaat agcgaaagtg accctgtacg
     3841 tagacagcat tttactaagc agttagtttt attgcccaca gcgaatatct caaccttaca
     3901 tgctttttgc ctgacagtta ttcgacgtta ttattactta attgatattg atccagtctt
     3961 tcgcatgtta acagatgaaa cagaaacgat cttgatgaag gaggatgtgt gggacgagct
     4021 gagagaagca ctttatgcag aaaatgatga acgatttttc caattaacaa tgaatttttc
     4081 taatgatcgt tcagatgacg gcttgacgaa tctggtgttt tctctttatg agtttgcccg
     4141 tgccaaccct gacccccaaa aatggctaga acagttaagc gataattatc gtttgcctga
     4201 ggggctagcg aaatctcgat tatatcaaaa acaaatccgt cctttagttt tggcagatat
     4261 ataccaatgt gtacagttat atgaacagat gactcagtta gcacaaggtg aaggattaga
     4321 aaaaatgcat gagcaagtag ctggagaaca acagcagatc aagaatatct atgaagcgtt
     4381 ctcgcaagat cggttagaag aagcttatgc tggtttggaa caactaactt tttcaacttt
     4441 taagagcagt cggaaagcag aattgaaaga aatttccaat gaagtcaagg gcatgaggga
     4501 taaagcaaaa aaactgattc agcagataag taaaagttat tttcctgttt ccccaagcca
     4561 aatggaagaa ttaacggaca aagcactacc gttagtcgaa gaaatgacaa aagtcacgca
     4621 aagcttcatg gacggtttca gtatgcgtaa acgggaaaaa ggtgtgctgg atttcaatga
     4681 tctggaacat ttagcgttgc agattctcac agaaaaaacg aaagatgtat ggctgccaag
     4741 tgaagcttca aaacattatc gaaaaaaatt caaagaagta atggtagatg aatatcaaga
     4801 cgtgaaccaa ttacaagaag ccattttata ctggctaaga gaacccgatg atacaaaagg
     4861 aaatatgttc atggttgggg atgtcaaaca atccatctat tctttccgtt tagcagatcc
     4921 tagtttattt atcgggaaat acgagaattt ctcaaagaaa gaaggcggac gtcgaatcgt
     4981 tttggctgag aacttccgct ctagaaaaga agtcctttct tttacgaatc ttatttttga
     5041 acaactaatg gatccagcag tgggtcaaat caattatgac gaagcagcca aactgatcca
     5101 aggattttca gactttccag agaatgaaca gtttgagccg gaaatcatga tctatgaaaa
     5161 agaacaggaa gaatcagaga ttgagattcc gacggatgat atactggaag acaaaacaga
     5221 aggcgaactt tttatgacag gactaaaaat tcgtcaatta attgattcct cctttatgat
     5281 ttatgataaa aaatcaaaaa aaagtcgtcc gatagagtac aaagacattg ttctcttgac
     5341 acctacaaag aagaacaact taacgatttt agagattttc aaaacattgg atattccatt
     5401 agagatgaat gatgctcaaa actattttca agcgacagaa attcgcacga tgatctcgct
     5461 gctacaatta atcgataatc cctatcaaga tattccgctt gcggctgtat tgcgctcacc
     5521 gatcgtgggt ctgattgaac cagaattagc cagcattcga ttagctaacc gtgcacatac
     5581 ttactatgat gctgtgcttg cttatcaagc atcaaacgaa gatgaattag cagctaagct
     5641 ggaacatttt ggcaagcaac tagagcattg gcgagaactt gctcgccgtt cttcgatcac
     5701 tgacttgtta tgggatatct attatgagac gggatatcta gagtatgtgg tcggtctgcc
     5761 agcaggggca cagagacaag ccaatcttta tgcattggtt gatcgtgcca aagcttacga
     5821 gcaaagttct ttcagaggat tataccagtt cgtgcgtttc attgaaaaaa tgcaagaaaa
     5881 agataaagac ttagcagaac cagtcatttc aatagaagat aatgctgttc gcgttatgac
     5941 gatccatgca agtaaaggct tggaattccc agttgtattt ttgttagata tgacaaagga
     6001 gttcaactta caagacttgc gcaatcggta tgcttttgaa gaaaaacttg gagcaggaat
     6061 tcgctatatg gatcctgaaa cacgagtgtt atatgatacg ttaccgtttc aagccatcaa
     6121 attggcaaag caaaacaaat tgttgtcgga agaaatgcgt aaattgtatg taggattgac
     6181 tcgggcagaa caaaagcttt tcattgtcgg ttcctataaa aataaagagc aaatgatcca
     6241 aacgtggtct gaagcagctg atcatgagga gttagttttt gatcctgcgc tacgtttgaa
     6301 gggcagaagc agcttgatga attggattgg ttacggattg atccgtcatc ctgaaatgca
     6361 gaaatactta gaggaagaaa tttcgacttc tcttcttcaa cattcaaatg cacagttttc
     6421 tatttcatgg atgaatcagc aatcgatcat tgaacaaaga cagttgcttg ctgaaaaaga
     6481 actggttaac ttagatcagc agatgaaaga agatgaaact ttattagcag attcgttaca
     6541 aaaacgtctg gcatatgaat atccttatca agcttcttca caaaccacaa gttatcaatc
     6601 tgtatctgaa atcaaacgat tattcgaaga tccagatgat acacaagaat cacgtttgac
     6661 tctggaaagc agtcagaaca aagcagcatc ccgtcaattt cgttatacgc aagaacaact
     6721 agcagaacca aaatttttgc aaaaagatcg gcaagtgtca gcagctgctg ttggaactgc
     6781 tacgcatgct ttgttacaat tactgccatt agagatgcca actacagaga gtatccatca
     6841 aaaattacag gaattggtaa ggaaacggtt agtagacgaa aaagtcgcga aaaaagtaga
     6901 tgtttcttct attatatggt tctttcaaac agaacttggt caacagctga ttgccaacaa
     6961 agaaaacgta aaacgagaac aacctttttc catgctgctt cctgctgacg aagtatttca
     7021 agactatcca aatcaagaag atgaactatt aatccacgga atcgtcgatg gatacctaga
     7081 agaaaaagat catctcaata tctatgattt taaaacagat tttattttac atccggatga
     7141 tccagcagaa atagatgcaa tcgtccaaaa atatcaagga cagctaagac tttaccaaca
     7201 agcaatgtca gaagctttga ataagccagt agaaaatgtt ttcctaattt tgcttcgagt
     7261 gaaacaaatt ataaacatta acaaataatt ataattgagc aaacttgact tatgttttgt
     7321 aaggattatg ctatactctg ggaagaggtg agtatgatgg agctaacaca gacagaattc
     7381 gttttatgcc ctaaatttga aaaaacattc tctattttag gaaaaaaatg gaacggattg
     7441 attatcgatg tactactcga aaacggtccg caacgctttg ttgagcttgc ggcaaaaatc
     7501 ccgatggtca gtgatcgagt attagtcgaa cgcttgaagg aattagaaaa agaaggaata
     7561 gtcactcgaa actttgcctg tagcgaaaag agccgttcag aatatctctt gactgaaaaa
     7621 ggagcagact tgcaaaaagc gatgcgtcaa atacaagttt ggagcgaaaa atggatcaca
     7681 gaggaagaat gcggttgatt ttgtaaaaaa aatcttgtaa actatagtcc atacgaataa
     7741 gtaaagaacg atgacgaaaa agagtagttt ggctaaacca gttcagggag atctgtcgca
     7801 gactgaaagc agatcctgtg tttgaaaaat gaagttcact ttccgagttc gtctgataaa
     7861 atcagaccgg caattgtcgt taacgaagta agagtgcggt gtaagtcatt tatccgcaaa
     7921 ttaggatggt aacgcgaaga cctcgttcct taacaggaat gaggtctttt tgttttgtat
     7981 ttggaattta aaggaggaac aagcacatgt ctctacaagc acaattagag agcttgaaag
     8041 aagcaacgct cgtcaaaatc acagcggcac aagatttaaa tgaattaaat caaatacgtg
     8101 ttgaaacgtt aggaaaaaaa ggaccgatca cagaagtatt acgtgggatg aaagatctaa
     8161 gtccagaaga gcgaccaaaa gtcggaagtt tcgcaaatga gatccgagat cttttgacag
     8221 aagagatcga gcgaaaaaaa caagtattag aagaagaagc gttaaatgca gctttggcaa
     8281 aagaaacaat agacgtgaca ctacctggta gaaaagtaaa acatggtaca cgtcatatcg
     8341 tgacacaaat catggaagaa attgaagata tttttgtggg tatgggctat cagatcgtag
     8401 aaggatatga agtagaatct gatcattaca atttcgaacg gatgaatctg ccaaaagatc
     8461 atccagcaag ggacatgcaa gatactttct atatttcaga cgagatattg attcggacac
     8521 atacttcacc agtccaagca cggacaatgg aaaaacatga tttctctaag ggtgctctac
     8581 gaatgatctc gccagggaaa gtcttccgca gagatacaga tgatgcgacc cacagtcatc
     8641 agttccatca aattgaaggg ctagttgttg ataaaaacat cacaatgggc gaccttaaag
     8701 gaacattaga agtcgtaatg aaaaaaatgt ttggggaaga tcgtgaaatt cgtttgcgtc
     8761 caagttattt cccatttacg gaaccatcag tagaagtaga tgtcagctgt ttcaaatgtg
     8821 gtggtgccgg ttgtaacgta tgtaaataca ctggatggat cgaaatttta ggagctggca
     8881 tggtacaccc gaatgtgttg aagatgtcag gaatcgatcc agaagaatat tcaggctttg
     8941 ctttcggcct tggaccagac cgcgtagcta tgttgcggta tggagtgaat gatatccgta
     9001 atttctatca aaatgactta cgtttcttaa gtcagttcaa gggggagtaa gaaaatgtta
     9061 gtttcatata aatggctcaa tcgttatgta gatttatcga atgtaacacc aaaagaatta
     9121 gcggataaga tgtctgtgac aggaatcgaa gtagagggag taacggttcc agaagaaggc
     9181 ttgaaaaaaa tcgttgtcgg tgaagtcaag gaatgcgtac cacatccaga ttctgatcac
     9241 ctttctattt gtcaagtcga tattggcgaa gacgaactgt ctcaaatcgt ttgtggagcg
     9301 cctaatgtga aggttggaat caaagtgatt gttgcacttc ctaactcacg tatcgctggt
     9361 aatgtaaaaa tcaaaaaagg aaaaatgcgt ggccaagtat caaatggcat gatttgctca
     9421 ttacaagaaa ttggctattc agatagtgtt ataccgaaag aatacgctga aggtatctat
     9481 tatatgccac aagaagctgt aaatggcgat ccagtttttc cttatttgga tatggatgat
     9541 tcgattattg aattatctat cacaccgaat cgtgcggatg cattgtcaat gcgtggcgta
     9601 gcttatgaag ttggggcaat ttatcgtcag actcctacat tcgatgacga aaagctggta
     9661 gaatcctcac gtcctgctag cgataaaatt caagtggaag ttgaagataa agaagcagtt
     9721 cctgcttatc aaatcagaat cattgaagga gtaagaatcc agccaagtcc tcaatggctg
     9781 caaaatcttt tgatgaacga aggcattcgt ccaatcaaca atgtagtaga tgtgacgaac
     9841 tatattttgc ttttattcgg acaaccacta catgcatttg actacgacaa attgaaatca
     9901 aaagagatct ttgtccgccg tgcaaaagaa aatgaaacat tagtaacact agatggagaa
     9961 tctcgggaat tgacaactga caatctcgtc attactaacg gcaaagaacc agttgcttta
    10021 gctggtgtca tgggagggtt agactcagag atcacgaatg aaacaacaac agttgcacta
    10081 gaatctgctt tatttgatcc aatttcgatt cgtcgtacag caaaacaatt taatttgcgt
    10141 agtgaatcct ctgcacgttt tgaaaaagga atcaaccatg cgactgttgg agaagccagt
    10201 gaggttgctg cagcaatgat cgtttctctt gcgggtgggg aagttctaca aggcgcggtc
    10261 aaaggctcag agatcaaagc agaagacgta tcagtgaaga tttcacttga acgcatcaat
    10321 caatatttag gaacatctct tgtcgttaaa gaagtaaatg agatttttga agcacttgga
    10381 tttagctatg aagaaaagga gtatgtttat tccgtagtag taccaccaag acgctgggat
    10441 atccaaatcg aagcagatat tatcgaagaa gttgctcgta tttatggata cgaccagttg
    10501 ccatctacac ttccaagtgg agaaacggtt gctggaagct tgtcaaaaga acaagaaact
    10561 actcgtaaaa ttcgtacaat ctttgaagga agcggattga gcgaagcaat cagttatgca
    10621 ttgacaacag aagaaaaatc aagacagttt gttataaatg aaagtaatat tacacgtttg
    10681 gactggccaa tgagcgaaga acgttctgta ttacgaatga atttgatcag cggactatta
    10741 gatgatatca gttataacac tgcccgaaaa aatacagaga ttgctttata tgaaattggt
    10801 cgggtgttct tccaagaaaa tgatccacgt acacatctgc cgcaagaagt aaaacatgca
    10861 gcaatcgcta tttctggtat ttgggaagaa aaagattggc agacgaaagc tcaaaatgta
    10921 gactttttca ccatcaaggg tcttttagaa aacttatttg aacaactagg catcactgaa
    10981 gatgtccatt atcaaacagt gacagaaatg aaggaaatgc atcctggaag aactgctgcg
    11041 atttaccttg gcgaaaaatt tattggtttt gtcggtcaag tgcatccaac gattacaaaa
    11101 agttatgata ttccagaaac atatgttgct gagtttgatt tatctgctat agtggaagct
    11161 gctcaaaaag ggatcgtttt cgaagcagtg acaaaattcc cagcagtcag ccgggacatc
    11221 gctttgcttg taaaagaaac tgtgacaaat caggaattaa cagaagttat ccgttctgct
    11281 gccggaagat ttttaacaga tatccaatta tttgatgtct atcaaggaga aaacattgaa
    11341 aaaggacata aatccatggc gtatagcctg acattcgtca acccagaagc aacattgaca
    11401 gatgaagaaa tcaataaagc aatggaaaaa gtcgaaaaag cgttggtaga aaacctagag
    11461 gcaagtattc gctaagaagg aaaatatagt aagtaacgat caagtagctt ttgaagacca
    11521 tacatttgaa aaaaagatgt gacacaactt cgtcacatct ctttttttga ttttaaaaaa
    11581 ggaatagcac agagacgaga taaaaaaaca ggcctttaca gcctgctttt ttagagtaaa
    11641 tgaattaatg gaagtaattg attcccattg ctgattttac ttcagaaagt gtttgtgcag
    11701 ctactttagc agcttcttca ctacctttac gtaagatttc cataacagct gaaggatctt
    11761 tttccaattc ttctcggcgc gcacggatcg gtgcaaattc agcttccaat acatcaatga
    11821 ggtatcgttt gatctttaca tcaccaagtc cgccgcggcg gtaatgagct ttcatttctt
    11881 caattgcttc tttatctgtt ccgaatacat ccaagtaggt gaagaccata ttcccttcta
    11941 cttgtccagg atcttgtaca tggatgtggt ttggatcagt atacatactc atgacttttt
    12001 tctgtaagac atcggcagga tcggagatat aaataccgtt tcctaacgat ttactcattt
    12061 tgccatttcc atcgattcca ggtaatctac ccattccttt tggtgggaat acgccttttg
    12121 gttccaccaa gacttcacca tatgtatgat tgaagctttg tacgatttcc tgtgtctgct
    12181 ctagcattgg tttttgatcc tcaccaacag gtacaagatt cgctttgaat gcagtgatat
    12241 ctgctgcttg agaaacagga tagataaaga atcctgtagg cacgctttca ccgaattttt
    12301 tttgttcgat ttctgttttg acagtcggat tgcgccgaac gcgtcctacg ctgactaagt
    12361 tcaaataata catggttaat tcagctaatt ccggaatttg tgattggata aataaagtag
    12421 attttgcagg atctaatccc acggctaaat agtctaaagc aacttgcaag acatttgatg
    12481 aaaccttctc aggattttta gcattatctg tcagtgcttg catgtcagca atcatgacaa
    12541 acagttggtt gtcttcatct gcctgcatag ccactcgggt ttttagagaa ccgacgtaat
    12601 gccctaaatg cagcttccca gttggtcgat ctcccgttaa aataatattt ttcaaaaaag
    12661 acacctcttt acgatttgta agtctgagca cttaatatct taccgttttt cctgaaaaga
    12721 aacaacagtt cctaaggaaa aaacaaggaa agaggataaa ataactaata gattagaaat
    12781 ggaagaaata tttatgggag aataaacgtt ctaagaaact ttacaaatta ctaaactatc
    12841 agatataatg tattttatta tacatcttta aaagaaatct tactaaataa taatgggagt
    12901 gtaattctat ggcaatgatt aaatttgatc acgtggataa atattatggc aagttccacg
    12961 cattaaaaaa tatcaatctc gaattcgaga aaggagaagt tgtcgttgtc attggtcctt
    13021 ctggttcagg aaagagcacg atgcttcgct gtatcaatgg attagagacg atctcttcag
    13081 gtaagctgct gattaatgat actgacttgc atgataaaaa gacaaaactg acagaagtcc
    13141 gaaaaaacat cggcatggtt ttccaacatt tcaatttgta cccaaataaa actgttctgg
    13201 aaaacatcaa attagcacca atcaaagtgt tgaagcaaga ccaggcaact gcagtcaaaa
    13261 acgcggagaa atttttgaaa actgtcaata tgttagataa gaaagattct tatccttcca
    13321 tgttatccgg gggacaacag caacgggtgg caatcgccag aggtttagcg atgaatcctg
    13381 agatgctgct gtttgatgaa cctacttctg ctttggatcc ggaaatgatt ggtgatgtat
    13441 tagacgttat gaaaaaattg gctcatgacg gtatgtcaat gattgtcgtg acacatgaaa
    13501 tgggctttgc caaagaagtg gcggatcgtg ttatcttcat ggctgatgga gaagtattgg
    13561 aagacagcag aaatgtgaga gactttttcg aaaatccaaa tgaagtccga gcacagcaat
    13621 ttatcagtaa agttatcaat cattgatagg aggaaaaaga tgcgcaggtt aaaaaaaata
    13681 cgcctgattt tccttttagg cattgctttt cttgcactag ccggatgtaa aggaaatggg
    13741 ttggcaaatg aagatatttt agagcgaagc aaatctacca atgaaatcat ctggggtgtc
    13801 aagtatgata ctcgattatt tggaatgatg gatattgaga gcagaactgt acaaggattt
    13861 gatgtagata ttgcaaaagc aatcactaaa aagatccttg gtgacaatgg aaaaacagaa
    13921 tttgttgaag tcacttctaa aactcggatt ccattattga aaaacggaaa tatcgatgca
    13981 attattgcaa cgatgacgat cacagatgaa cgaaaaaaac aggttgactt ttcagatgtc
    14041 tatttcgatg ctggccaagc tttgttggta aagaaaggaa gccaaatcaa aagtgtagat
    14101 gatttgaatg cttctacaac ggttctagca gtcaaaggtt ctacatcggc tgcgaatatc
    14161 cgacagcatg caccagatgc aaaaatttta gaattagaaa actatgcaga agcatttact
    14221 gctttacaat ctggtcaggg agacgcgatg actacggata atgcgatttt gttagggatt
    14281 gcagatgaaa atccagagta tgagttagtt ggtggaacgt ttactaatga gccttacggg
    14341 attgcgatca acaaagggca agaaaacttc ttgaaagctg ttaaccaagc gctagaggaa
    14401 atgcatgcag atggcacata cgataaaatc tatcaaaaat ggtttccaaa tgaaactgaa
    14461 ggaaaagtag agtaaggggg agaggcaatg agcgaattga tacaaactta tggaccaatc
    14521 tttgtcgacg ggttcaagat cacattgttt tcgagtgtct tagcacttgt gtttagtttg
    14581 attatcggaa cgttaatggc aatcttccaa ttatcaaaga ataaagtagt gagcgcctta
    14641 gcaaaggcat atgtagaatt ttttagaaac attccattat taatcatcgt gatgtttttt
    14701 tatgttgtcg taccgattta ttggtttaat ctagatggat tccaagcagg tacgattgga
    14761 ttaacgatct atacatctgc ttttattgct gaaacagtac gttcagggat tcaaaccgta
    14821 ccaaaaggtc aaacagaagc aggactatct tctggtttta cctattcaga aatcatgcgt
    14881 tatattgttt taccgcaagc attcaaaatc gttatcccgc cattaggcaa ccaatttatc
    14941 aacctcgtaa aaaactcatc tattttagca atcgtagctg gtttagattt gatgtatcaa
    15001 ggagacttga tcgctagtga aacattcaat accttcgata cgtatatcat cgttgggtta
    15061 ttttacttag ttatcacgct accgttatct tacttaatgg tttatcttga gaaaaaatgg
    15121 gcggttcgtt catagaagga ggggaaatac atggatttta gcggcgcttt ttcatggatg
    15181 aatatccgtt ttttattgga aggattaaag gttactgttg aagtttcatt gttttcgatc
    15241 atttttagtt tcttgatcgg tggtctgaca ggtgtgattc gttttgcgaa tatccctttc
    15301 ttttccaaaa tccttggtgt agtgattgat attatacgga atcttccatt attattgatc
    15361 attttcttca cttactttgc cttacctcaa atcggcattc agatgaacat cttctggtcc
    15421 gctgtcgcag cattaacgat ttttgagtca gctatgttgt cggaaatttt ccgagctgga
    15481 ctcaatgctg ttccaaaagg acagatggaa gcaggattat caacgggact gacttatgta
    15541 gaatcgatgc gaacgatcgt gttaccgcaa gcatttaaat caatgatccc ggcaattgtg
    15601 agccaattga tttcactgat caaagatact tcattagcag taatcatttc tttacctgaa
    15661 ctttctcatc aggcacgtat tatttatgga cagaacacca attatgtgct tccgatgttc
    15721 atcatcatga cacttatgta cttcgtggta tgttacgcgc tttctctgct ttccggttac
    15781 ttggaaaaga gacggtacag ttattaatag atgattcttt tagcggttgt ggcaaactaa
    15841 agtcacaacc gcttttcctc tttccattcg aaaaaaagag atttcgattg ttttaagcaa
    15901 gtaagagcac ttgaaaattc acgctaaaaa gtacacaata gggaggatag aatttattga
    15961 aaaaaaggat gatacgattg acagataatc gccctatcgg atttattgat tcaggtgtcg
    16021 gcggcttgac tgtagtaaaa gaagccctga aacaattacc gaatgaaaat attttatttg
    16081 taggagacac agcacgctgc ccatatggcc ctagacccgc ggaacaggta atccagtata
    16141 cttgggaaat gacggattat ctggtggagc aaggaatcaa gatgctggtg atcgcctgca
    16201 ataccgcaac tgcggtggct ttagaagaaa tcaaagctgc tctttctatt ccagtcatcg
    16261 gtgtgatcct tcccggtact agagcggcag taaaaaaaac acaaaataaa caagttggca
    16321 ttatcggtac gattggtacg gtaaaaagtc aagcttatga aaaagcactg aaagagaaag
    16381 taccagaatt gactgtgaca agtcttgctt gtcccaaatt tgtttcagtt gtcgaaagta
    16441 atgaatacca ttcatcggtg gcgaaaaaaa ttgtggcaga aacattagct cctttaacca
    16501 ctaaaaaaat cgatacattg attttgggat gcacccatta tccattatta cgccccatca
    16561 ttcaaaatgt aatgggagaa aatgttcaac tgatcgattc tggagcagaa acagtaggtg
    16621 aagtatctat gctgttagat tatttcaatt tgagcaattc accgcaaaat ggtcggacat
    16681 tatgccagtt ttatacaact ggctctgcca aacttttcga ggaaatagct gaagactggc
    16741 ttggaatcgg acacttaaat gtagaacata tcgaattggg aggaaaataa ggatgcgtca
    16801 tgacgggaga aaagcccaag agcttcgcaa actggagctt caaacaaatg ttttcaaata
    16861 tccagaagga tcggtagtga tccgttttgg tgatacaaca gttatttgtt ctgcaacaat
    16921 agaaaattct gttccccctt ttttgagaga aacaggaaca ggatgggtga cagcagaata
    16981 tagtatgctg cctcgagcaa cgagtacgag aaatcgtaga gaaagcagca aagggaaact
    17041 ttctggtaga acaatggaaa tccagcgtct tattggtcgt tccttgcgtg cagtagtaga
    17101 cttggaaaaa ttaggagaac gtagtatcat cgtagactgc gatgtgatcc aagcggatgg
    17161 cggtactaga acagcaagta tcactggggc atttgtggca ttgaaattag ccattgaaaa
    17221 acttttaaga gaaaaagaat tgtcagaaga tccaataaaa gaacatcttg ctgcagttag
    17281 cgtaggaatc ttaccagatg ggacttgcgt cacagatttg gattaccaag aagattccgc
    17341 agcacttgta gacatgaatc ttgtaatgac agaatctgga aaattcgtgg agattcaagg
    17401 aactggagaa gaagcaacgt ttgatggtga acagttgaat gaaatgctct tttttggaaa
    17461 aaacgcgatt gaagatttga tcaaagagca aaaacatgct ttgctaacag agtttgctca
    17521 aaatgatgag cggatagaag aaacaaaaac aatcatcatt gcgacaagaa atccaggaaa
    17581 agcagaagag tttcgaaata tgttcaaaga agcgggatat catgtaaaaa cattacttga
    17641 ctatcctgaa ttgccagatg tggaagaaac tggcagcaca tttgaagaaa atgcccgatt
    17701 aaaagcagaa acgattgcac aattgcttga tcagccagtc ttagcagatg attcaggttt
    17761 gaaagtggat gctttaggcg gtatgccagg aatctattct gcacgttttg caggggaaca
    17821 aaaaagtgat gcggggaata acgccaaatt attatatgaa ttgacagatg tgccggatga
    17881 aaaaagaaca gcacaattcc attgtacgtt agtattcgcg gcacccaaaa aagacagcct
    17941 tgtagtagaa gcggaatggc ccggtcgagt agcccgtata ccatctggcg aaaatggctt
    18001 tgggtatgat cctttattta tcccagaagg taagaaacaa acagcagcag aattatcgag
    18061 cgaagaaaaa aacaagatca gtcatcgggc tcaagcaatg aaaaaattga gtgccgagtg
    18121 gaaacaatgg ctggaaggag agagatagat gcggtattta gtagtaagtg ataatcatgg
    18181 agacagagat atcctcgttg acttagtaga tcgttatcgt tctgaagtgg atatcttttt
    18241 ccattgtggt gattcggaat tagaagcttc tgacccatta tgggatacat tccaagtggt
    18301 tcaaggtaat tgtgattatg gaccaggatt cgaacagaaa aaagtgatcc aaactggtca
    18361 ggatacagta ttcatgactc acggtcattt atcgaatgtc cgatttggtt tgacacagtt
    18421 agcaatcgaa gctcgagctg caactgccga tatggtgttt tttggtcata cccatcaaat
    18481 aggctgtgag atggatcaga aaatcctgtt tttgaatcct ggtagcattt ctcagcctag
    18541 aggcccgatc caaatcccat catatgctct tatcgaaagt ttagatgacc aaatttctgt
    18601 tcaatactat aaccgtgccc atcaggcagt tgaagagttg gccttttctt tttcgagaga
    18661 gcgctgaata aaaaaactct aatttttaat aaaaataagc tttattttat gaattttgca
    18721 tccggtttcg gtaaaatagt agtgttgaat gaaatagaaa tggaggccat cagatgattg
    18781 gacctatagt aagagaaatg ctgctggaaa atcaggaaac gtttttgatt cctgcagaaa
    18841 atgtggcaaa tgttatgtat catcatccat tgtcccatgg tttacttgtg ttatcgaagg
    18901 tcggttattc caaaattcct gtattaggaa aagatgatca ctttgtagga ctagtaagtt
    18961 tatcagatgt ggtcaataaa atgattgact tgcaaacaat cagcatggac ccgttagaag
    19021 gactaacggt tgctgatgtg acagaaacag atgttccgac aattgatgaa aattgggaac
    19081 tagaagaagt actccatttg ttagtcgatt cgcctttttt accggttgtc acagaagatc
    19141 ggattttcaa aggaatcatt acaagaaaag agctgcttaa agccgtaaac ttcatggtgc
    19201 atgaattaga acgtcgtaat atattgtctc cgaaaatcga agaattaaaa gaacaaatcg
    19261 atatcgtgag ctaaagaacg agaaactaaa agataatcaa aaaataagta gaaaaaacag
    19321 cgtaccagtt ttgactttat cttgaaaaag aggctgggac aaaagcgttt aactccaaga
    19381 aataagaagg aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg
    19441 aaggagttgc ttctgcttcc gccgtttata cgtgtttaga gcgtgggaca aaagtgttct
    19501 ttacttttgt cccacgctct ttttagtgag attcgttgca tcgtttgtcc aaattatttt
    19561 ccgacagcaa ttggactgtt cggaatcgtg aaaccagaag ctgtcaattc tttgacatag
    19621 gagctcaaga actcttcttt taaggcatat tgttttccgt tcaatgcgta gcagaccgta
    19681 cgaatcgcaa aattgctgtt tccaagatcc actaatccaa agatagaagg ttcagtttgg
    19741 agctcttctt ggtatttttc agctaatgta cgatttactc gatcaatgat ctcatagatt
    19801 ttgtcatatc cttcttcagg aacaatccga atatctaaaa gtacttgcat atttgcacga
    19861 gagagattgc tgatcgtggt gatgtttcga ttcgggatga aatggacagt accatccgtt
    19921 gctttcagct gaagggtacg gataccaaca gatacgacgg ttccttcaat cgttaagttt
    19981 gctagcttga tgtagtcccc aacatccatc tgttgttcca tgataataaa aaatccggta
    20041 ataacatcac tcatgaaccc ttgggcacca agaccgattg ccacacctgc aattccggca
    20101 ccagccaaaa gtgatccgat cggcacaccg atagcagata aaattgcata gataaaaaag
    20161 aaaccaaggg tataatgaaa aatagtattt aaaagactat gtaaagtctt taagcggctc
    20221 tcgtttagaa tggctttttt agaatactga ttaaaagact tatcaatcag atattttccc
    20281 acacggtgaa gaatccaaaa aaggacgatc aaaaaaatca gatataaact tttttggata
    20341 aaggcagcca aaatggcgtc ccaatcaatc ccgtcccaaa accgctgaaa cgcatttact
    20401 ttgctgacag cttgatctgt caatgtctcg ccagaagagt ctgtcgtacc ggcagttgct
    20461 aataatgaaa acataaaatc ctcttttcta aaatttgatt ctttctgtat tgtaccaaga
    20521 agttttcaaa aaaggaaata gcaaaaataa ctataatgaa ggaaaaaagc tgaaaataaa
    20581 atgacaaata atatggataa tgataaaatg tggagaaaat aacgaaggtg gtaagtatat
    20641 gcaaatcgat tggaacaatc taggtttttc ttatatcaaa actccttggc gttttattgc
    20701 caaatggaaa gacggacaat gggaagaagg agaattgaca gaagataatt acctgacgat
    20761 tcatgaaggc tctccagcgc ttcattatgg tcagcaatgt tttgaaggtc taaaagctta
    20821 tcgtcgaaaa gacggcaagg tcaatctctt tcgacctgaa caaaatagtc gacggatgaa
    20881 tcaaagtgct agacgattgc tgatgccaat ggtccctgaa aagatgttcg ttgatgctgt
    20941 caagcaagtg gtaaaagcaa atgaagattt tgtgccgccc tatggaacag gcgctacatt
    21001 atacttgcgc cctttactga ttggggtcgg tgaaaacatt ggtgtccatc cagctccaga
    21061 atatttattt gtcgtcttct gtacgccagt aggagcgtat ttcaaaggtg gactaaaacc
    21121 aacgaatttt attgtatctg attacgatcg tgctgctcct aaggggactg gtgcagcaaa
    21181 agtcggcgga aattacgcag cgagtttact tccaggaaca gaagcaaaag aactggatta
    21241 ctcagattgt gtttatcttg atccagccac acatacgaaa atcgaagaag taggtgcagc
    21301 aaatttcttt ggaattacaa aagacggaaa attcatcact ccgcaatcgc cctctatctt
    21361 accaagtatc acgaaatatt ccttgttgca gttagctgaa gaacgactag gactgcaagc
    21421 aattgaagga gatattttca ttgatgatct agatcaattt gcagaagctg gtgcatgtgg
    21481 tacagcggct gttatttctc caatcggtgg gatttatcac aataacaatc ttcatgtatt
    21541 ttatagtgaa acggaagtag gaccagtaac aaaacggttg tatgatgaat tgacaggtat
    21601 tcaatttgga gacatagaag caccagaagg ctggattgaa gtcgtggaat aatttcaaag
    21661 taatgagttg aaaaaaacgt aaaaagaaaa aatcaggaag agactttaaa aagttttttc
    21721 ctgattttta tatgttttac tttaaagaat gcaacaaaag tttatgaaag atggacacct
    21781 ttgtcaacgt agataatatc accgatgatt ccgctggaaa gtgggctgat taagaaagca
    21841 caagcattac cgacttcttc gatcgtcaca ccttgctgat caggcgtacg agattcagat
    21901 aactggatca gtttttgata gtctttaaca cctgtgacag ctaatgtttt gattgcccca
    21961 gctgagattc cattgacccg aattccttta ggcgagaact cagctgccaa ataacggata
    22021 gcagcttcaa gagaagcttt cgcaataccc atcatgttat aattcgggat tgcgcgttct
    22081 gaacctagat aagtcatgct aacgatcccg ctgtttggtg caagaaacgg ttgtgcgtat
    22141 tttgatacag caattaaaga gtagctgctg atatcttgag ctaattgata gccgtctctg
    22201 gaaatatcag acacatttcc ggacaattct tctttgttgg cgtaagcaac tgcatggaca
    22261 aggccgtgga tctccccagc atattcaccg atggcattca ttgctttttc gatgctttcg
    22321 tcgctagaaa catcacattc tacagtgaat gcttcttcgc cgaccaattt gactaatgat
    22381 tttttcattc gttcattttg gtaagtatag ataatttctg ccccttgttt caacattgct
    22441 tcagcacaac cccaagcaat gctttttttg ttagcgacgc ccataatgac gatttttttt
    22501 ccttctagaa atgacataat gactctcctt tgtttctgat aaaaatttga tagtcgtaaa
    22561 aaaatagaga ataatagcta aaataacttc ttgatttctt aattattagt caaaatgttt
    22621 tgattgtcaa actatttttt cttaaattaa aaaaagattg ctaaatattt aggaatatga
    22681 tactttgata gtcaaagtaa tgaaaatggc aattgcctga aagaaggagt ttaattatgt
    22741 cttacgtatt atctgctact gaagtaatgg aattgattcc taatcgttat cctatttgtt
    22801 atattgatta tgtagatact ttagaaccag gaaaaaagat cgttgcgaca aaaaatgtaa
    22861 caatcaatga agattttttc caaggtcact ttcctgacaa tccagtgatg ccgggtgcgt
    22921 tgattctaga aacactggca caagcaggtt cgatacttat cctaaaatca gaagagtttc
    22981 taggaaaaag agcttatatc ggtgggatca ataaggccaa attccgtcaa aaagttgttc
    23041 caggagatgt gctgaaatgt cattttgaaa tcaccaaaat gaagggatca gtcggtacag
    23101 cgttatctgc agcatatgta gatgataaaa aagtctgtga gtgcgagttc acgttcatcg
    23161 tcaaccaaaa tgcttgacta taaaacctga tatagcaaaa caaaaggaag cttacttgct
    23221 ttttatgtaa aaagaagacg cctgaatcct agcatacgtt gtcatctatg caggattcag
    23281 acgttatttt tttatttttg ttttgcttct tgcagtatat tgattttatc gatttttact
    23341 tcttcttttg gtttgtctga actatctgtt tcgatatttg caatagcatc cacgacatcc
    23401 attccttcaa tcacttggcc gaagacagta taatggccat ctagtgaagg ataaccgccg
    23461 tttttataag cgtcgatgat tgcttgagga tagtcgtctt ttaaaagacc atcatgcata
    23521 tcatctgtat tttgaacgat aaagaactgg ctgccgttac tgttaggatc ttgtgagcga
    23581 gccatagata acgcacctct tatattataa aggtggttgt tgatctctgt ttcaaatcct
    23641 tctccccaaa tgctttcccc tccagtgcca tcgcctttag gatcgccacc ttggatcatg
    23701 aaatctttga tgacgcgatg gaaagtcagt ccgttgtagt aaccgtcttt tgcatgtgtc
    23761 ataaagtttt caaccgcttt tggtgcgtgt tcgggaaata atttgatttt gattgtgcct
    23821 ttattcgtca ccatttcgac cagatcttca ttttcggcga catctttcgt caattgtggt
    23881 aattccaatg cgttcaaatc gacgctactg gcagtcgtgc tttccgcagt agatgtactt
    23941 tttgaacttt ctgtagacga actagaagaa gtcgtgttgt ttgcagaaca accagcagca
    24001 aacagcaatg ctgctgctaa actaatactt aaaataactt tatttttctt cataactgtt
    24061 cctttccaat ttactatttt gttatcttta atgataacaa aatagaggga aaatacc
//
LOCUS       DAHHPA010000040        23976 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.041, whole genome shotgun sequence.
ACCESSION   DAHHPA010000040 DAHHPA010000000
VERSION     DAHHPA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.041"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            79..831
                     /locus_tag="NBH55_001419"
     CDS             79..831
                     /locus_tag="NBH55_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1509410.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            828..1742
                     /gene="pfkB"
                     /locus_tag="NBH55_001420"
     CDS             828..1742
                     /gene="pfkB"
                     /locus_tag="NBH55_001420"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCD1509411.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1760..3691
                     /locus_tag="NBH55_001421"
     CDS             1760..3691
                     /locus_tag="NBH55_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1509412.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3802..5361)
                     /locus_tag="NBH55_001422"
     CDS             complement(3802..5361)
                     /locus_tag="NBH55_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCD1509413.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5516..7750
                     /gene="pcrA"
                     /locus_tag="NBH55_001423"
     CDS             5516..7750
                     /gene="pcrA"
                     /locus_tag="NBH55_001423"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCD1509414.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7767..9803
                     /gene="ligA"
                     /locus_tag="NBH55_001424"
     CDS             7767..9803
                     /gene="ligA"
                     /locus_tag="NBH55_001424"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCD1509415.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9909..10214
                     /gene="gatC"
                     /locus_tag="NBH55_001425"
     CDS             9909..10214
                     /gene="gatC"
                     /locus_tag="NBH55_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCD1509416.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10214..11686
                     /gene="gatA"
                     /locus_tag="NBH55_001426"
     CDS             10214..11686
                     /gene="gatA"
                     /locus_tag="NBH55_001426"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCD1509417.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11683..13113
                     /gene="gatB"
                     /locus_tag="NBH55_001427"
     CDS             11683..13113
                     /gene="gatB"
                     /locus_tag="NBH55_001427"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCD1509418.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13123..14178
                     /locus_tag="NBH55_001428"
     CDS             13123..14178
                     /locus_tag="NBH55_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD1509419.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14289..15662
                     /gene="rlmD"
                     /locus_tag="NBH55_001429"
     CDS             14289..15662
                     /gene="rlmD"
                     /locus_tag="NBH55_001429"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCD1509420.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15665..15793
                     /locus_tag="NBH55_001430"
     CDS             15665..15793
                     /locus_tag="NBH55_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCD1509421.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            16233..16421
                     /locus_tag="NBH55_001431"
     CDS             16233..16421
                     /locus_tag="NBH55_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCD1509422.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            16499..17512
                     /locus_tag="NBH55_001432"
     CDS             16499..17512
                     /locus_tag="NBH55_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1509423.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17579..18613)
                     /locus_tag="NBH55_001433"
     CDS             complement(17579..18613)
                     /locus_tag="NBH55_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1509424.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            18728..20953
                     /locus_tag="NBH55_001434"
     CDS             18728..20953
                     /locus_tag="NBH55_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCD1509425.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            20971..22854
                     /locus_tag="NBH55_001435"
     CDS             20971..22854
                     /locus_tag="NBH55_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCD1509426.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            22835..23809
                     /locus_tag="NBH55_001436"
     CDS             22835..23809
                     /locus_tag="NBH55_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCD1509427.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         8017 a         4380 c         5190 g         6389 t
ORIGIN      
        1 tttatgaacg tttttgactg atttatattg cgagtagaaa aaaaatatgc tattatttgt
       61 attgaggtga tcgtgaaaat gcttacagag gaacgtagac aaaaaatcct agacttgctt
      121 gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc tttttaatgc ttctgaatca
      181 acgataagac gtgatttaca agagctggag aatagtgggt ttctagaaag agttcatggc
      241 ggagctaaga aagagcaatt attgaatttt gagcagaata tgagtgaaaa atcactcaaa
      301 aacgttcacg aaaaacagca aatcgccaaa tcggcagctg aaaccattaa tgacgaagat
      361 gtcatttatt tagacgctgg ctctactacg ttagaaatga ttccttttct gcaaggaaaa
      421 cagatcacag tagtgacaaa ttccgttcaa catgcggcaa atttagttga tttggagctt
      481 gctaccatta ttttaggtgg aacgataaag ctggcaacta atgctgtttt aggatctaat
      541 gcgatagaac aactaaaaaa ttttcatttt aataaagcat ttatgggaat gaacggtgcc
      601 catcttgaac aaggattcac gactccggac ccagaagagg cagctgttaa acgtttagcc
      661 atgaaaaatt ccgaagagac atttgtattg attgatcata ccaaattcaa ccagttgtcc
      721 ttcacctctg ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg tccattagaa
      781 ttcgttaatg aattccgtga aaagacaaca attaaggagg caatacaatg atttacacag
      841 taacacttaa tccatcaatt gattatatcg tacgcgtaga tgggttaaaa ctaggcgatt
      901 tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa agggatcaat gtttcccgta
      961 ttttgaaacg gatcgattct caatctacag cactagggtt tttaggaggg ttcactggcg
     1021 aatttatttc gaagtggctt gaaaaagaac aaatccagtc tttgttcaca accgtaaaag
     1081 aagatacaag aatcaatatc aaactaaaat cagatactga aactgaaatt aacggtttgg
     1141 gcccatctat ttcagatgaa gaaatcgatg agttgaagca agcattagga acagtaggac
     1201 aaggagatat cgtcgtattg tcaggaagcg cgccggctag cctgcgaaaa ggattttatc
     1261 aagaactgat tgatatcatt cgagaaaaag gtgcagattt cgtgattgat acaacaggtg
     1321 atgacctaaa aaatgcactt gttaagaaac cattgttgat caaaccaaac aatcacgaat
     1381 tagctgagct ttacaataca accttccaat ctgtagaaga aatcattccg tatggtaaaa
     1441 aattgctagc tgaaggggca cagaatgtgc tgatctcaat ggctggagac ggtgctttgc
     1501 tcttcacgga acaaggaact tataaaacga atgtattagt tcgcccattg aaaaattcag
     1561 taggtgcagg ggattcaatg attgccggct ttattggggc ttacagccgt acgaaagatc
     1621 caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc aacagctttt tctgatgact
     1681 tggcagctcg cgaattgatt gatgagttgc tgccagaagt tgaaatcaca aaaattgatt
     1741 agtttcaagg aggataatca tgaatatcaa ggatttaatg gtcaaagaag cgatgatcat
     1801 ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa aaatgtttga
     1861 tgccggccgg atttccgata tcgaaacata taaagaagga attctagccc gcgaagcaca
     1921 aacttctaca ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca gtgcagttaa
     1981 agaagcaaca gttttattcg caaaaagcca aaaaggagta gattatgaag ccttagacgg
     2041 acagccaact tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg acacacattt
     2101 gcaagcatta gcagcactat ctcgattgct gattgatgca gatttcgtag gaaaattaaa
     2161 agaagcaaat acaccagaag aagtccaaga cttgttccaa acagctgaag agcaaaaaga
     2221 agcagaagaa aaagcagaac aagaaacaca agctgcagaa gcttctgctc atccagaaag
     2281 aaaattcgtt gttgccgtta cagcttgtcc aacagggatc gctcacactt acatggcaga
     2341 agatgctctg aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg aaacgaacgg
     2401 ttcagaagga atcaaaaacc gattgactgc tgaagacatc gcccgtgcag aaggtgtaat
     2461 cgttgcagca gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac tgatcaatcg
     2521 tcctgtcagt gatggtatcc gtaaaccaga agagctgatc aatctagccc ttagtggtac
     2581 tgcaccagta ttccatggtg atggaaaaga aagcagttca gaagaaggaa atgcggatgg
     2641 tactatcgga caaagaattt acaaagattt gatgaacggc gtatcacaca tgttaccttt
     2701 cgttattggt ggaggaatcg ctattgcgct ttctttcatg atcgaccaat ttattggcgt
     2761 gccacatgat caattatcaa atctagggaa ttacaatcaa gctgctagct ggttcaacca
     2821 aatcggacaa gctgcatttg gtttcatgct gccggtatta gcaggattta ttgcttcaag
     2881 tatcggtgac cgccctggtt tgatcgttgg atttgctgct ggtgcattag ctaatacagg
     2941 cggtgcagga ttcttaggtg cactgattgg tggattctta gctggttatg tcatcgtatt
     3001 tttacgtaaa ttattcaaga acttaccaaa atcattggat ggaattaaaa cgatcctctt
     3061 ttatccagtg tttggcttac tgattactgg tttcttgatg ttgctggtca atgtccctat
     3121 gaaagcaatc aatgatgcat tgaacggttt cttgacaagt atgagtggtt cgaatgctgc
     3181 tttacttggt gcattattag ctggaatgat ggcggcagat ttaggcggtc caatcaacaa
     3241 agccgcttat gtgtttggta cagcaacttt agctacgact gttgcaactg gcggaagtgt
     3301 cgtgatggct tctgttatgg ctggaggaat ggtacctcca ttggcaattt tcgttgctac
     3361 acgtttgttt aagaataaat tcagcaaaac agatcaagat gctggtttga cgaacatcgt
     3421 gatgggacta tcgtttgtca cggaaggatc gattccattt gcagcagctg acccgattcg
     3481 tgccattcca agttttatta ttggttctgc tttaactggc ggattagtcg gagctttcgg
     3541 gatcaaatta ctggctcctc atggcgggat tttcgttgta ttcttactga gtcacccggt
     3601 catgtatttg gtgtttattg ctatcggagc agtcgtatct ggtgtgattt atggtttatt
     3661 gagaaaagct cctgcagcag cagaagcata gaaataaaaa agaagaggct ggtttttccc
     3721 agtctcttct ttttttatag aatcatatag aatcaagtag tgtagatgaa tagttgttgt
     3781 aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa tttgtgatag aagatttagc
     3841 ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca gtcaagataa ccaaaatatc
     3901 tcctacattc atgatcgtat caccgtgagt caagatttcc tgatttcctc tgcgtataga
     3961 gattagcagc atatttttag gccaattgaa gtctcgtacc attaatccgt ctaaagaact
     4021 ctctgccgtt actggaaaat caaaagttgt ccttgcacct tttagtgagg cggtctgagt
     4081 atttccaagt agacgttcca ataatgattc gtaaattgga ttaccgccga gaaggtcatt
     4141 gacgatataa gcaacaagag cagccaatcc taaaggcatc agatgattaa gagagccaac
     4201 catttctgtc actaaaataa ttgctgtcag cggtgctttt ccaatcgctg tgaaataacc
     4261 agccatggca ataacgacaa aatccttgat gtagattgga tccatcccga aatcaaccat
     4321 aatgtttcca taaaagctac cgattaaagc ccccaatgtc aatatcggaa ggaaaatccc
     4381 ccctggaaga ccaccaccgt aagaaatcat ggaaaaaacg aatcgaagaa caaacaaacc
     4441 gataatcata atgattggca aggaagcatg tccaaaagct aaaacgattt gattgcctcc
     4501 gcctagagta ctaggccata aataaccaat tggcaaaatc agcaagaaag gaacgaaccc
     4561 gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt ggcaaagcca gcaagacctt
     4621 ctgataaata aaacctaaga tgcctagaag tactcctaag atcactaagg aaccataata
     4681 tttcaacggc aaagaaggaa ggtcacctag atataaaaca ggtctcaagc cgaaaaagta
     4741 aagagagacg aagttagcag tgattgctga agcaaatgat gtcagccaaa caagtggaga
     4801 aaaggagtga tggatttctt ctaaaacaaa gagtaatcct gcgataggag cattgaatgc
     4861 tgctgcaagt ccgctacttg caccgctaga aattaaaatc ttttcttctg aagatggact
     4921 ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca agctgtatag aaggtccttc
     4981 tctacctaga aaaaggcccg caccaactga aagtacaccg ccaatgaact ttttccataa
     5041 aacagagaac cagttcattg ataattcccc gcgaagctgt ccctctactt gtggaatccc
     5101 gcttcccttg atatccggtt catttttgac cagataaccc acgaaaaatg ctgcaaggat
     5161 gcagcaaata gctaggccaa ttaagtaaat cggctgttcc cgactcattt catagatact
     5221 tcgaactaaa tcgcttagct tttcgataga taatcgaaac aggctaacga cgattcctgt
     5281 cagcagtcca acaaatattc cttttagaat aaaatatatc tgagtcttat ctaatttctt
     5341 gatttttcct tttttttcca ttcaaacgcc tcgctttctt ttaatttagc gcgaaatcaa
     5401 cgaattgaca aggaaaaatc cataaaaaga aacaaatgat tttgaactag ctagtttaat
     5461 gaaagccaag aaaatgctat aattaacaag attgataaaa gaaaggatgt tgttaatgtc
     5521 atccaaagct gaattattaa acgggatgaa cccccgtcag aaagaagctg ttcttcacac
     5581 tgacggacca ttactgctaa tggcaggtgc aggaagtgga aagacgagag tgctgacaca
     5641 ccgcattgcc tatttgattg aggaaaaaga agtcaatcct tggaacatcc tagcgattac
     5701 ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc aatgcgattc tcgcatctgg
     5761 aggcgaagat gtctgggtat cgacttttca ctcgatgtgt gtgcgtattt taagaagaga
     5821 tgttgatttt atcgggtata accggaactt tacaatcatc gattcttctg aacagctgac
     5881 cttgatgaaa cggatactaa aagaactgaa cattgatccg aaaaaatatg atcctcgaag
     5941 tatattagga acaatctctc aagcaaaaaa cagcctgcag acccctcagg attttgcaaa
     6001 aatgcaaggc agttattacg aagagattgc ggcaaaatgt tatgctgctt accaaaaaga
     6061 gcttcaatat aatcaatgta tggactttga cgacctgatc atgaatacga ttcgattatt
     6121 tgaagaacat cctgattcac tcacatatta tcaaaacaaa ttccattata ttcatgtaga
     6181 tgagtaccaa gatacaaatc acgcacagta tacgctagtc aatttattgg caggtagatt
     6241 taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt tacggctggc gcggtgcaga
     6301 tatgcagaac atcttggatt ttgaaaaaga ttatccagat gctgcagtaa ttttgctaga
     6361 acaaaactat cgctccacga aaaacatcct tagcgcagca aaccaagtca ttgaaaacaa
     6421 tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa gaaggaaaca agatcactta
     6481 ctaccgtgca gataatgaac gagacgaaac ccgttttatc gtagatcgga tgcaagaaga
     6541 gattcgaagc aatcatcgga attacggcga ctttgccatc ttgtaccgga cgaacgcaca
     6601 atctcgtgta atggaagaaa ctctgttgaa agcaaatatt ccttataaaa tggttggagg
     6661 gcacaagttc tacgatcgta aagaaatcaa agatatttta gcgtatttga atgtgctggc
     6721 aaatcctcaa gattcaatca gttttgagcg tatcgttaat tcgcccaaac gaggcatcgg
     6781 accaggatct atcgaaaaac tacgcagttt tgcttctctt catgaatggc cactgcttga
     6841 agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa gcaggacagc aattaggtgc
     6901 atttggagag atgatccaag aagtcactca gatgatccca tacttgacag tgacagaatt
     6961 gacgaaagag gttttggatc gtagcggata tctagaagac ttaaaaatcc aaaatacatt
     7021 agaagcacaa gcacgcatcg aaaacttgga ggaattttta acggtcacac aagaattcga
     7081 caaacaattc gaacagcaaa atgaagaaga cgctgatgca ccagaagaaa aactaacggt
     7141 cttcttaaat gatctggcac ttgtttccga tattgacaat ttagaagaag atgcttccca
     7201 agtgacattg atgactttgc atgcagccaa aggattagaa ttcccagttg tattcctgat
     7261 tggtctagaa gagggggttt ttcctttatc tcgagcattg atggaagaaa gcgaactgga
     7321 agaagaacgc cgtctcgcat atgtggggat cacgcgagca gaagaagcgt tgtatttaac
     7381 aaatgctttc tccagaacat tatatggacg gacgcaatat aaccgacctt ctcgttttgt
     7441 agaagaaatc gatcaggagt tactggaaat cgaaggcatg cgtcctacgc ctaaaaaaac
     7501 acctgtattc gctaaaaaaa cagcatatag ctataaacag ccagagacag cggttgttcc
     7561 ctctaaaagt gctactggag gggaaaataa cagctggaaa cctggagata aagtcaaaca
     7621 caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg acgtcgaagg atctagaact
     7681 agatgtggct ttcccaagtc aaggagtaaa acgtcttttg gctgcctttg cgccaattga
     7741 aaaagcttag tagtggaggg taaatgatgg aacatgaaat gacgattgac gaagctgcta
     7801 aacgagcaga agaacttcgt acaagactga accaatggtc tcgcgaatac tacgtagaag
     7861 ataaaccaac agtggaagac tatgtttatg ataaagaata cgcagaatta gtagcaattg
     7921 aggaacaata tcctgatttg atcacgtctg attctcccac tcaacgagta ggcggaaaag
     7981 tattagaagg ttttgaaaaa gtcactcatg atattccgct ttacagtttg aacgacgttt
     8041 tcagcaaaga agagctgata gcttttgatc aacgtgtaca aaaagcagtg ggacgtgtag
     8101 ttgactattg ctgtgaacta aaaatagatg gactttccgt ttctttgcgt tatgaagatg
     8161 gaaatttcgt gagaggtgcg actcgcgggg atggaactgt aggagaaaac atcactgaaa
     8221 acctaaaaac tgtccggtcg gtcccaatca aattaaaaga accaatgaac atcgaagtaa
     8281 gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt gaaccaagac agagaagcag
     8341 agggaaaaga tatctttgcg aatcctcgga acgccgcagc tggttctttg cgtcagttag
     8401 attctaaaat cacagcgaaa agaaatctgg acactttttt atacacagta gctgatttcg
     8461 gtcctatgca agcaaaaacc cagtatgatg cgctagaaga gctagaaaaa attggattcc
     8521 acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga agtgtggtca tacattgaag
     8581 aatatcatga caaacgtgtg gatttgccct atgaaatcga tggaatcgtc atcaaagtga
     8641 atgaattctc gctacaagat caacttggtt ttactgtcaa agcaccgaga tgggcagcag
     8701 cttacaagtt tccgccagaa gaagttgaaa cgctaatcga aaacatcgaa tggacagtcg
     8761 gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc agtccgagta gcaggaacga
     8821 cagtaagccg tgccagcctg cataacggtg attatattaa gttgaaggat atccgcttga
     8881 aagatacagt tctgatttat aaagcgggag acatcatccc agaagtttcg caagtcgtgt
     8941 tagataaacg acctaaagac agcgaagaat accaattgcc tacacattgt ccagtttgtg
     9001 gaagtgaact tgtccatttg gatgaagaag tagccttacg gtgcatcaat cctaaatgcc
     9061 ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag aaatgcgatg aatatcgatg
     9121 ggttggggcc gcgcgtacta gaacagatgt atgataaaaa actagtagca gatgttgctg
     9181 atctttacaa attgacagaa gaagagttat tgacgttgga caaaataaaa gaaaaatcag
     9241 caaataatat tttgacagca atcgacaaca gcaaagataa ttcggtcgag cggttgattt
     9301 tcgggttagg aatccgacat gtcggagcga aagcagcaaa aattttagca gaacatttcg
     9361 gtgatctaga aacattgagt aaaagtgact atgagtcgat tattgcgctt gatacgatcg
     9421 gtgatatcat tgccgacagt gtagtcactt atttcagtaa cgaagaagtc catgaattga
     9481 tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa agggcttcga aatgcccaat
     9541 tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt ccttactggt aaactaacac
     9601 gatttactcg tgaagaagca aaagagacga tcgaaaatct tggcgggaaa gtgactggta
     9661 gcgtatcgaa aaaaacggat atcgtggtag caggagaaga tgctggcagc aagctgacca
     9721 aagcacaaga attaggaatc gaagtttgga cagaagatca aatggcagat gcattggcta
     9781 aaagtcgctc agtagaagaa tgaaaaatga gttttttcag acaaatttca ataaacaatt
     9841 tcagacacgg cactcttgtg ttaaaataga gagcaaagtt tttaactcgt ttagaaagaa
     9901 ggggtagtat ggcgattagt gaagaacaag tgaaacacgt tgccaaatta gcaaaacttg
     9961 cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt aggtaaaatc atcgatatgg
    10021 ttgaaatgct ggaagaagta gatacggaag gtgttccatt tacgtcgaat gtggttgaaa
    10081 cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg gaaccgcgat gaattattga
    10141 aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc cgcaatcatc gataatgggg
    10201 aggctggcgc ataatgacag aattacacaa tcgttctcta gaagaactgc atcatatgct
    10261 tgtatcgaaa gaaatcactg tacaagattt gacaaaaaca acatttgaac gaatcaaaga
    10321 aacagagcct gaggtcgagg cattcattac attgaacgaa gaaaaagcat tggaacaggc
    10381 aaaagcttta gatgaaaaag ggattgatga aagcaacgtc cttgctggta ttccaattgg
    10441 tatcaaggat aatatcgtaa caaaagatat cttgacaacc gctgcatcta agatccttta
    10501 caattttgaa ccaatttatg atgcaacggt catggataaa atctatcaag cagatatgat
    10561 ccctgttggt aagctaaaca tggatgaatt tgctatgggt ggaagtacag aaacttctta
    10621 tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta cctggtggct cttctggtgg
    10681 ttctgcagca gcagttgcag ctggacaaat tccagtttct ttaggtagtg atactggggg
    10741 aagtatccgc caaccagcag cttttaacgg catcgtaggg atgaaaccta cttatggccg
    10801 tgtctcacgt tttggtttga ttgcctttgc ttcatctctt gaccaaatcg gaccaatgac
    10861 ccgtacggtg aaagataatg cattagcatt gacagctatc agcggttatg acgaaaaaga
    10921 cggtacttct tcaggcgttt cagttcctga ttttgcagag ggactgacag gcgacatcaa
    10981 aggaatgaag attgctttac ctaaagaata cttaggcgaa ggtgtggccc ctggcgtaaa
    11041 agaagctgta ttgaaagcag cagaaacatt ccgttcatta ggtgctgttg tggaagaagt
    11101 cagcttgcct cattcaaaat acggtgtggc tgtttattat atcattgcct catcagaagc
    11161 aagttccaat ttgcaacgat tcgatggtat ccgctacggt tatcgctctg aaaatgtgaa
    11221 atcattagat gatgtatatg tcaactcacg ttcagaagga ttcggtgaag aagtaaaacg
    11281 ccgtatcatg ttaggtacat tctccttaag cgcgggttat tatgacgctt acttcaaaaa
    11341 agcaggacaa gtccgtacat tgatcaaacg agattttgaa aaagtctttg cagattatga
    11401 cttgatcatc gggccatctt caccaacagt tgcattcgga atcggtgaga acattaacga
    11461 tccaatcaca atgtacatga gtgacatctt aacgatccct gtcaacttgg ctggacttcc
    11521 aggtatgtct gtaccggcag gtttatcaga aggattacca gttggattgc agatcatcgg
    11581 taattatttt gatgaaaaaa cgatgtatca agcggcatat gcgtttgaac aagcaacaga
    11641 attccataaa caacaaccag ctattttagg agggaaagac gaatgaattt tgaaacagtc
    11701 attggactag aagtccacgt cgagttaaaa accaactcaa aaattttctc tccagcgcca
    11761 gctcattttg gtgcagaacc aaacagcaat acaaacgtga tcgactgggg ttatccaggc
    11821 gtgctgccag taatgaataa agctgcgctg gaattcggga tgaaagcagc tttggcatta
    11881 aactgtgaaa tctctaaaga tactcatttt gaccggaaaa attactttta cccagataat
    11941 ccaaaagcat atcaaatttc ccaatttgat caaccaatcg gtcatgacgg atggatcgaa
    12001 atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac gtgtccattt agaagaagat
    12061 gctggaaaaa acatgcatgg tattggcggt tattcttacg ttgatttgaa ccgacaaggc
    12121 acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt caccagaaga agcatacgct
    12181 tatctagaag cattgcgatc aattatccaa tttacagaag tcagcgatgt gaaaatggaa
    12241 gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc cttatggaca agaagagttc
    12301 ggtacgaaag cagaattgaa aaacttgaac tctttatctt tcgtgaaaaa aggtttagct
    12361 tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag gagaaatcca acaagaaaca
    12421 cgccgttttg acgaaacaac gaataaaaca gttttgatgc gtgtcaaaga agggtctagt
    12481 gattatcgtt acttcccaga gcctgatatc ccgaaattcg tgatcgatga cgaatggatt
    12541 caaaaagtaa aagctagttt gcctgagatg ccagcttctc gtcgcgaacg ctatatccgt
    12601 gagtttggac ttccagagta tgacgcaatg gtattgacac ttacaaaaga aatgtctgat
    12661 ttttttgaag ctgttttgga aaatggtgca gatgccaagc aagcatctaa ctggttgatg
    12721 ggtgaagttt ctgcttactt gaacagtgag aaactggaat tagctgagac aaaattgaca
    12781 cctgagaact tagcaggaat gattcgtttg atcgccgatg gaacgatcag ctcaaaaatc
    12841 gccaaaaaag tcttccgtga attgatcaca aatggcggag aagcccaaga agtagttgaa
    12901 aaaaatggtt gggtgcaatt atctgatccg aacaaactac tgccaatcat taatgaaatt
    12961 ttggataaca accaacaatc tgtcgatgat ttcaaaaatg gaaaagatcg tgcggttggc
    13021 ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag caaatcctgg tgtcgtgaat
    13081 aagctactta aagatgaatt agcgaaacga tagaggctga aaatgaaaaa agcacgcgta
    13141 atttataacc ccacctcggg aaaagaatta ttgaaaaaaa atttggcgga tatcctacag
    13201 gcattagaag aagcaggatt tgaagccagc gcctatgcaa ctacaccaga accagattca
    13261 gccaagaatg aagcatgtag agtcgcagaa ctaggttttg atctagtagt ggcagctggt
    13321 ggcgatggca caatcaacga agtggttaat ggaatcgctc cgcttgaaca ccgtcctaaa
    13381 atggccatca tcccagctgg tacaacgaac gactatgctc gagcattaaa aattcctaga
    13441 gataatatta aagcagcagc agaagtgata aaaaaacacc aaaccgtaaa aatggacatc
    13501 ggcctttctg ataacagtta cttcatcaat atcgctgcag gtggctattt gacagaattg
    13561 acttatgaag ttccatcaga actaaagagt atttttggtt atcttgctta tcttgcgaaa
    13621 ggagcagaga tgctccctcg agtaaagcca atcaaaatgc gcttgaagta tgatgaaggg
    13681 gaatatatcg gaaatgcttc gatgtttttc ctagggctga cgaactctgt tggaggattc
    13741 gaacagatcg cgccagatgc caaattggat gatgggaagt tttctttaat cattgtgaag
    13801 acagcaaata tctttgagat cttgcatatt gcagcactca tgctgaatgg cggtcgacat
    13861 gtcgatgatc caagaataat ctataccaaa acaagcagtt tgttcgttga aacagaagat
    13921 gatatcaatc gaccagtaat gatcaactta gatggtgaat acggcgggga agcaccgatg
    13981 catttccaaa atcttcatca acacattgag ttctttgcca ataccgatca gattcctgat
    14041 gaagcaatca caggtacaga tgaagaagaa ttagaggttg tcagcaaaga attcgtaaaa
    14101 gaagtcgaac gtttaacgga tgaagatatc gacggtgacg gaaaaatagc agacgaaaca
    14161 cctaatcaaa agaattaatc aaaagccggt gctgaacgta gtaaggtcag tgccggtttt
    14221 agttgtgtaa ttagctgatt tatagtagag ttcagtagga ttgaaaaata ggcttaaagg
    14281 agcaaaatat gacagaacag caaatagtaa agaaaaacga acgatataca gtcgaaatcg
    14341 tcgacttgag ttatgaagga atgggggtag caaaaatcga tggttatcca ttatttatcg
    14401 aaaatgcact ccctggagaa gaagtagaaa tactagtagt aaaagcaggc aataagtttg
    14461 gatatggaaa agtcgaaaca tttattacac aatcacctga tcgtcagcct gtagaaaaca
    14521 atgcattact tcgcataggt atcgcacctt tagcccattt gaagtatgaa caacagcttg
    14581 tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat tgctaagatg ccagaagttc
    14641 ctgtacttcc aacggtcgga atggcagaac cattcggcta ccgtaataaa gcacagatcc
    14701 ctgttcgccg tatcgatgga cagttagcca ccggttttta caaaaagaat agccatgatc
    14761 tagtccctat cgaagatttt tatatccaag atcctgtcat cgatcaagca atcaaagtag
    14821 tcagagatat cttgcaacgg ttccaagtac gaggatataa tgaagaaaaa aatgaaggac
    14881 agcttcgtca catcattatc cgtcgtggtc attattccca tgaaatgatg gtcgttttag
    14941 ttactcgtaa agaaaaattc tttaaatcca aggaaattgc tgaagctatt catgaagaat
    15001 tgccagaagt ggtttcggtc atccaaaata tcaatgaaga aaaaacaaat gtgattttag
    15061 gagacaaaga aaaagtttta tatggcagag actatatcga agatcagcta ttaggaaaaa
    15121 cctatcgaat ctcagcaaag tcattttatc aagtaaatac tcaacaagca gagtatctgt
    15181 accaaacagc catcgagttt gccgagttag ataaaaaaga cattgtgatc gacgcttatt
    15241 caggaatagg tacgattggc ttgtctttag cagacagagt ggataaggtg tatgggatgg
    15301 aagttattcc agaagcaatt gaagatgcac agttcaatgc tttaaataat aaaatcgaaa
    15361 atgttcatta tgaagtcgga aaagcagaaa aagttatgaa aaaatggcaa gaaaaaggga
    15421 tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg attagatcat cattttattg
    15481 aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat ctcatgtaat ccggcaactt
    15541 ttgcaagaga tgctaagtta tttgcagaag caggttatgc cgtgaagaaa gttcagcctg
    15601 tagatctttt cccgcaaacc catcatatcg aattagtcgc ttcattcgtt aaaggagaat
    15661 aaaaatggac caaaaagaaa aaatgaaaga attgatcaaa aagaaacaag gtggaggccg
    15721 atccaaacaa atggaaacac ctaagaatga tcgtaaaaac atgagaaaag gccctaaaat
    15781 ctttaataaa tagacgtgct aaagaaagac taagaagtgt taacttttgt tattgtttct
    15841 tagtcttttt agttagtcta cattattttg gcgatttgcc aataaggact gttgagttgt
    15901 gcaaaatcaa atctgggtca gaatgaacgt cattctgtct cagatttttc gttatttcaa
    15961 tttgattaac tgattgatca aaaagatttt tattttcata ttctcaaatg attcaacact
    16021 aaatatacat gtttgacgcg gaaattatta ttcgattttg gcggcatcag gggggatgac
    16081 gacgtagcag acccattatt ttactcaaga aaatgtccgt gcctattatt tagaatacga
    16141 tagtgaacgt gctgggagtt ttgttctttt agagaaagtc tctgaagata aattagttgt
    16201 cttagatttt gactgtaacg aaaaatggcg aaatggaaga gcaccagcaa gtgattgacc
    16261 ggatctacga agcgacacga tatctgcctt tggatcgttt gtgcttaagt cctcaatgtg
    16321 gatttgcttc aacagaagaa gaaaatgttt tgtttgaaga acaatggaaa aaatttcttt
    16381 tgtcaaaagc attgcagaag aagtttgggg aaaatcatta aatcatctag gagagcttga
    16441 taggaaataa cactatggta taatcagaat gcttaatact aactactgag ggagccttat
    16501 gaaaaagaaa attcttaaat ttatcggatg gttcttcgga atcattcttg ggatcgcgtt
    16561 tgcaatcttt cttgccttcc agctttcccc cagacctgga gcattcttga tcaaccatat
    16621 gttttcaaat actgttcaaa tcactgataa aaaaacgttc gatcaagcag aaaaaaacgt
    16681 ggcgaaaaaa acagatttga cgtatgagtc caagtataaa gagaatactt atgacatcta
    16741 ctatccaaaa aacagcaagg ggcctgttcc tgttctattt tgggtacatg gtggaggatt
    16801 cgtcggcgga gataaatcgg gtgtcaaaga atttgcgaca aaattagttt cagacgccaa
    16861 catcgcagtg gttgcgatga attatgaatt agcgcctgat tcagaatacc ctaatcaagt
    16921 tcttcaagtg aatgaactga tcaaagattt attagctgaa gaaaaagacg acaaattgct
    16981 agacatggaa caattatttt ttggtggaga tagtgcaggt tcacaaatcg cattgcaata
    17041 tgcagctata caaacgaatg atgcgtatgc taaacaaatg aaaatcaaac atctacttcc
    17101 aaaagatagt ctgaaaggaa ccatttctta ttgcggacct gttgatttga agctaacagc
    17161 acagcagcat tcggatgatc gatttatgaa gtttttcgtc aaaaccgttg cgtggtcgtt
    17221 gattggaaca aaagattgga aaacgagtga gcaattgaaa gaagctagtg tggctgacca
    17281 tgtttctaag gaatttccgc caacgtatat aactgacgga aatgcatact cattccagga
    17341 gcaaggtcaa gcattagaaa atcggttgac agaattaaag gtacccgtgc aatccttgtt
    17401 ttatacagat acaaaaaaag agattaccca tgaataccaa tttgattaca cgctaaaaga
    17461 atcgcaaaat tgttaccagc agacattgga ttttgtgaat aagtataagt aagaggttgt
    17521 gaaaaagccg tttcgttttg agtatgaaga aacgaagcgg cttttcacaa cctctctttc
    17581 aatacctttt tgttcttggg ataaaacttt ccctaacaat caatcgtgca ggataagtca
    17641 aaatgattgg ttcgtcccgt ataccgtcaa ttcgctccaa tgccattcga acagctgctt
    17701 gcccaattga tttcgcattg atttgaacac tggtcaagcc aggggagagg tttgcgattg
    17761 tctcgatatt atcaaagctt gctacaacaa tctcttctcc aatttggata tttttttgtt
    17821 tcaatgcccg atagacacca attgccattg gatcgctccc gacaagaatg gcagtaggaa
    17881 gaggggattc attttctaat agattagtcg caagtacctc accttgtttt tctgtccagc
    17941 cacctagatg ataagacata tatttttcta agtgatgatc gatcataaaa tgctgatagg
    18001 cacgcatcct cttttcattt atagaaatct gcttcccacc gaattcatcc atatcgactt
    18061 tatatccccc gatataggct attcttttat gaccattttc taagaacaat tccagtacgg
    18121 ttttagtcat ccgttcaaga tctgcataca ccatatcgac ctcttgctgg atgtcagggt
    18181 tatccacgac gactatttgc atgttttgtt ttaacaaggc ggtaatcgat tctttcgtaa
    18241 cagtcccaac aacgatcacc gcacctaact gatctagatc ttcccattgt ttaggattat
    18301 cagatagatt atagattcga ttcatcccta aatgcagacg tcgagcttct tcttccatcc
    18361 ctactcgcaa atgtttgtaa tagatatcct gttcttcgct cgctttgccg atcgtagaga
    18421 tcacaccaat cgaaggcatt ctctttttga tatatttacg agtactgatg tcataatcca
    18481 acgcagcagc tgcctctact atccgttgcc gagtagtctg agccacatgg atcgtctgat
    18541 cgttattcaa cacacgggaa actgtagcag gagagacatt tgcctttcgt gcgacatctc
    18601 taattccaac catttttaca cactcctcaa aaaaataaaa aatttttatt gacaaaattg
    18661 tttagtaaac agtatacagg aattataaaa aaaagtaaac ggtttccgtt ttgttgggag
    18721 tgaaatgatg gatattacgc atagaaaaac agataaaaat ttcatcttat ttgatgaaaa
    18781 gagccgagtc ttccatttac gaaatacatt tctttcttat ctgataaaga tagaagaaag
    18841 caatgtattg gcgcatattt attttggcaa accagtcaag cagtacaaag acaataaaaa
    18901 ttatccccga agagatcgtg gattttcagg aaatgttccc ttaaatcctg accggtctct
    18961 gtcgaaagat acactaccgc aagaatactc ttctcatggc agcatggact ttcgaacgcc
    19021 cgcatcgatc attcagcgca agaatggatc agacttactg gatctgagat atgacagcca
    19081 ttatattaca gatgggaagc cagagatcga aggcttgcct caaacttatg tgatggacaa
    19141 gtcagaggca caaacgttgg tgatttcact taaggataga gaaacagcga tttatttcga
    19201 tttgttctat accatattta ctgatagagc agtgatcaca cgatctgtaa agataagaaa
    19261 tgaaaccgga gaaacaatca aactagaaaa agcggctagt ttccagctag actttgccca
    19321 tactaggcga tttgatgaag tcattgcttt gccaggagca catgtcaatg aaagacagat
    19381 ttcacggcaa tctgttttat ctggaacaaa agtatttgaa agccgtcgag gaacgagcag
    19441 tcaccatatg aataacttta ttgctttagt tcatcatcat acgacagaaa atactggaga
    19501 agcgatcgga cttcaatttg tttattccgg caaccattct ttcgaacttg aaaaagatca
    19561 gatcaatcaa ttgagagtcg tcggtgggat caacagccat cgtttttcgt gggaattgaa
    19621 tgcaggccag tcttttcaaa cacctgaaat gatcctttct tactcaagtc agggattgaa
    19681 caaaatgagt cagatccacc atgagttgct acgtgaaaga atcgcaagag gtcgtcatca
    19741 atttgctgaa cggccgattc tggtcaataa ctgggaagcc acttactttg acttcaatag
    19801 tgaaaaaatc aaggcgatca ttgatgaagc aaaggagcta ggcatcgaaa tgttcgtatt
    19861 agatgatggc tggtttggaa aaagagatgc ggacaattct tcattaggag actggttcga
    19921 gtatgaaggc aagttgacaa atggattacg tgaaatagca gattatgctc atagcaaagg
    19981 attgaaattt ggtttatggt ttgaacctga aatgatctcg gttgattctg aactgtatcg
    20041 aacacatcct gattttctta tgcaggagcc aggtagaatg ccttctgctt ccagatcaca
    20101 acatgtactg gattttacaa ggttagatgt tcggcagacg attgagaaac aaatgagaaa
    20161 gattttagat acgattcctc ttgactacat caaatgggat atgaaccgtt cgctttcaga
    20221 tgtctattcg attacccttg acccacagag acaaggagaa gtagcgcatc gttatatgct
    20281 cgggctttat gaactacttg aacatttgac aactgattat ccggaaatct tatgggaagg
    20341 ctgttctggt ggaggtggac ggtttgatgc cgggtttatt tattatatgc cgcagtcatg
    20401 gacaagtgat aacacggatg cagtggagcg tatgaaaatc caatatggaa caagccttgc
    20461 ttatccgatt tcgtccatca cagctcatgt atcagctgta ccaaatcatc agaccgacag
    20521 atctacttcg ttgaaaacaa gaggagaaac agctatgagc gctgtttttg gttatgaact
    20581 ggatttgaca aagctgtctc ctgaagaaga aaagcaagta aaagaccaaa ttatatccta
    20641 tcaaacgatt cgtccggtga tccagtatgg ccattattat cgtttggcga gtccttttga
    20701 agaaaatatt gccgcatgga tgtttgtgtc tcctaaacaa gatgaagcta ttgtgttttt
    20761 agggaggata ctagcttcag ctcaaccagc gttccatgaa gtatatctga tggggttaga
    20821 tgatgaggca ctttatcagg aacagacctc gaagcggata ttttcggggg ccgaattgat
    20881 gacagttgga ctttacttcc ccgattttca aggtgatttc caaacagaac tgcttcattt
    20941 caaaaagtta tgagagagaa ggaaaaaagt atgaaagcaa atataaaatg gttagatgat
    21001 ccacaagttt ttagagtgaa ccaatcaaat gcacatagcg atcatgtttg ttatgcaaca
    21061 agggaagaag tagaaaccac atcctccctt aaacagtctt taaatggatc atggcgtttt
    21121 tgttattcgc caaatccttc ggtacgacca aaaacatttt ataaaaaaga ttttgatgcc
    21181 tgttcttttg atgaaatcac tgttcctagt catattgaat tagcaaatta tgacaagatc
    21241 cattacatca atacgatgta tccgtgggag ggacatgtat ttcgccgtcc ccctttcacg
    21301 acaagccctg agaaagaggc ggaagggatc ttttcagctg ctaaagacaa tccggttggt
    21361 tcgtatttga cgacttttcg attagaagaa ggtctaaagg gaaaacaaat ctcgattcat
    21421 tttgctggtg tagaacaagc aatgtttatt tggttgaatg gtcattttat cgggtatgcc
    21481 gaagattcgt tcactccgtc tgaatttgat ttaacacctt atttggaaga agaaaatatt
    21541 ctagctgtgg aggtttacaa aagaagtaca gctgcattct tggaagatca ggactttttc
    21601 cgattttttg ggatcttcag agatgtcttc ttagttgctc gaggacaaaa ccatttagaa
    21661 gatctttgga tcaaacctcg cttagatgac aaatgtcaaa atggcaaact tgaagtagat
    21721 ttgtcttttg agcaagtagc caaaaacagt cagctgaatg tagcgatact agatcctgat
    21781 gggaaggaaa tctatcaaca gcaatatccg ttaaaagagc aaatgacgat tcgcatcgat
    21841 ggatttgaaa atgtggcact gtgggggcat tttcagccca atttatataa gcttgaattg
    21901 gagacgatag acgaagaaaa aagagtaacg gagtatattg actattcctt cggttttcgt
    21961 aaaatcgaaa tcaaagataa aatcatctac ttcaatggaa aacggatgat tatcaacggg
    22021 gtaaatcgac atgagtggca tccgaaaaaa ggacgcgcca tcactataga agatatggaa
    22081 aaagatttgg ctatcataaa aaatgcaaag atcaacggcg tacggacatc tcattacccc
    22141 aaccagatca cttggtatta tttatgcgac caagcaggaa tccatgtgat ggcggaaacg
    22201 aacctcgaat cccatggctc atggcaaaaa atgggcgcat ttgagccttc ttacaacgta
    22261 ccaggtgcag tacctcagtg gcaggcggct gttcttgatc gagcaaaaac gaatttcgaa
    22321 acattaaaaa accatccaag tattcttttt tggtctcttg gaaatgaatc ttatgcggaa
    22381 gaaaacattc gacaaatgaa cgattacttc aaaaaagtag accccgatcg acttgtccat
    22441 tatgaaggga attttccaaa tccagcattc gaagattcga tttcagatgt aaaaagctgc
    22501 atgtatgcgc caccagaaga gatcattgac taccttgaaa ataaagcgga aaaaccgttc
    22561 atcttatgtg aatatatgca tgacatgggg aattcgttag gtggaatgga ttcttacatg
    22621 acgttgcttg atcgttatga aatgtaccaa ggaggctata tatgggattt taaagatcag
    22681 gcgatccaag tgactgatcc aatcacaggc agagaagtga tgcgctatgg cggtgacttt
    22741 gacgataaac caagtgacta tgaattctca gggaatggga tcgtttttgc agatggacaa
    22801 gaaaaacccg ccatgcagga ggtaagatat tattatgaaa aatacagtaa ataaaagtca
    22861 tatggatacg gaaaaagttg caatcgtctt cggcgactgt acattaggtg tcaaatcggg
    22921 gaatacgcat tatatttttt cttatacaag aggcggactg gaatcgctca ataaaaacgg
    22981 aaaagaatgg ctataccggg aaacacttcc agttttttgg cgagcaacga ccgataatga
    23041 cagaggtagc ggatttggat ttaaatccgg tatgtggtta ggagccggat tgtatccaaa
    23101 agtcaaagaa atagaagtac tggtggacaa taaaaagatc gatttgccga ttgctccagt
    23161 aaataatcaa tattcaagta atgaatacgc tgaaacggtt gaaatcattt ttacactgga
    23221 aatcaacaca gtaccaaaaa cccaagtgac aatttcctac caaatcgctg gagagggtaa
    23281 aatgcttgtc aatatgcatt attatggaca aaaaggatta ccggaattac ccgcattggg
    23341 tttaaggttc attatgccaa cgaaagcaaa attttttgag tatgaaggcc tatcagggga
    23401 aacatatccg gatcggatgg ctggtggtgt cgaaggtatt tataaagtgg caggactccc
    23461 cgtttcccca taccttgttc cccaagaatg cggcatgcat atggaaacga aatgggtaga
    23521 actaacaaga aatcaaacgt tggataatac ggataaagat caagaagatt tttcgttgcg
    23581 atttgaacag caggatacgc catttgcttt ttccgcttta ccattcaaag cagaagaact
    23641 ggaacaggca acacatatag aagaactacc gccagaaaga cgaacggttc ttacgatttt
    23701 tgcaaaagta agaggagtcg ggggtatcaa tagctgggga gcggatgttg agtcacctta
    23761 ccgaatcagc agcgaagagg accatgaaat cggctttatt attcagtagc aatctttaga
    23821 ttattatttg aaaaacggag tttacaagac aaagctgctt tattctatat gtcatgaaca
    23881 agagagtaaa aaagttctca gatacttttg ttcatgacta ttttcttcaa taaaaccaat
    23941 tgttattttt ctgaaaaaca aagttaacta tcagaa
//
LOCUS       DAHHPA010000041        23698 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.042, whole genome shotgun sequence.
ACCESSION   DAHHPA010000041 DAHHPA010000000
VERSION     DAHHPA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.042"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="NBH55_001437"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="NBH55_001437"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCD1509428.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="NBH55_001438"
     CDS             complement(1172..1867)
                     /locus_tag="NBH55_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCD1509429.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="NBH55_001439"
     CDS             complement(1880..2596)
                     /locus_tag="NBH55_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCD1509430.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="NBH55_001440"
     CDS             2926..3534
                     /locus_tag="NBH55_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCD1509431.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="NBH55_001441"
     CDS             3859..4182
                     /locus_tag="NBH55_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCD1509432.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="NBH55_001442"
     CDS             4364..5734
                     /locus_tag="NBH55_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCD1509433.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="NBH55_001443"
     CDS             complement(5864..7165)
                     /locus_tag="NBH55_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD1509434.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="NBH55_001444"
     CDS             complement(7519..9477)
                     /locus_tag="NBH55_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCD1509435.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="NBH55_001445"
     CDS             complement(9654..9920)
                     /locus_tag="NBH55_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD1509436.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="NBH55_001446"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="NBH55_001446"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCD1509437.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="NBH55_001447"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="NBH55_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCD1509438.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="NBH55_001448"
     CDS             13264..13827
                     /locus_tag="NBH55_001448"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD1509439.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="NBH55_001449"
     CDS             complement(14080..14601)
                     /locus_tag="NBH55_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCD1509440.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="NBH55_001450"
     CDS             complement(14604..15446)
                     /locus_tag="NBH55_001450"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCD1509441.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="NBH55_001451"
     CDS             complement(15439..16164)
                     /locus_tag="NBH55_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCD1509442.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="NBH55_001452"
     CDS             16340..16636
                     /locus_tag="NBH55_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509443.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="NBH55_001453"
     CDS             17204..18796
                     /locus_tag="NBH55_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCD1509444.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="NBH55_001454"
     CDS             18809..19549
                     /locus_tag="NBH55_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD1509445.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="NBH55_001455"
     CDS             19717..21066
                     /locus_tag="NBH55_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1509446.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="NBH55_001456"
     CDS             complement(21194..21784)
                     /locus_tag="NBH55_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCD1509447.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21931..23283
                     /gene="brnQ"
                     /locus_tag="NBH55_001457"
     CDS             21931..23283
                     /gene="brnQ"
                     /locus_tag="NBH55_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD1509448.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6793 a         4772 c         4511 g         7622 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caagaactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaccgtaaa ggagttatgt atgacaaaaa aattatcatt taaagactat
    21961 ctttttatag gctccatgct tttcggtcta ttttttggtg ctggaaatct tatctttccc
    22021 gttcatttgg gtcaagaagc aggcgctgct accttttggg cgaatctagg tttcttggtt
    22081 accggtatcg gtcttccctt cttaggggtg attgccattg gtgtatcgaa aagctcaggt
    22141 gtatatgaat tagcaaaacg gataaatcaa acatatgcga tggtttttac catcttgctt
    22201 tacttagtta tcggaccgtt ttttgcttta cctcgccttg caaccacatc tttcgagatt
    22261 ggtttagcac catttatcga tcctagcaga cagacgctat atctagcaat tttcagtatt
    22321 gtattttttg tgctggcttg gtggttttca agaaaaccga caaagatatt agattatgtt
    22381 ggaaagttcc tgaatcctgc atttttggtt ttattaggtg ctttgcttct ccttgctttt
    22441 ttgaatccac taggttctgt aaatcaagcg cctattcagc caaattatca agagcatgct
    22501 tttttcacag ggtttactca aggatataac acattagatg cgcttgctgc tttagcattt
    22561 gggatcatca ttgtcacaac aatccaaaac atgggtgtaa caaaacctgc tgaaatcgct
    22621 aaagacacga tcaaatctgg tgcgatcagc attatattga tgggaatcat ttatactttg
    22681 ctcgcttatt taggtgctat gagcttaggt agctttgctc taagtgaaaa tggcgggatc
    22741 acgctggctc agattgctga tcattattta ggtacttatg gcagtatctt attagcattt
    22801 atcgttatat tagcttgctt gaagaccggt attggtttga ttacagcttt tgcagaaaca
    22861 ttcacagatt tgtttccgca aagaaattac gcattctttg ttgcacttgc tagtatcctt
    22921 ccttgtttag cggcaaacgt aggcttgacg aatatcattc aattttcgtt gcctgttttg
    22981 atgttcatct atccattagc aatgacactg attttactag tgttagttgg tcctttgttt
    23041 aagcagcgta cggctgttta tcgaatgact acttatttta cattgattgc ttcgattttt
    23101 gatgggttga acgcttgccc agaaactatc aaacaaacac ctattgttca aaatctttta
    23161 tatgttgcgg aaagctactt gccgtttttc aaattgggga tggggtggat cgtaccagca
    23221 gttatcggtt ttgttatcgg actgatttgg agtttcgcta agaaagaaga agtagcagat
    23281 tgataaaaga ggttgtgaaa gagacgttca gccttgagca ataaaaagta attttgaaaa
    23341 atggcttctc acatttttgc ataaatttaa cttaataccg agaaactgac ttttgaacac
    23401 cgtttattga gaggtcgtga aaaagccgct tagtttcaag tattaagaaa tattttgtga
    23461 aaatggcttc tcacattttt gcataaattt gacttaatac cgagaaactg gcttttgaac
    23521 accgtttatt gagcgtctat aaactgacct gagtattaga gaaacaaaac ggcaaagtag
    23581 ctccatatac tttgccgttt tgttttgaac taataccgaa gagaagctga tcaaacaaca
    23641 tttcttttat aatatagatt ttagacatat atatgatata aaaatagttt tcgcaaaa
//
LOCUS       DAHHPA010000042        23412 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.043, whole genome shotgun sequence.
ACCESSION   DAHHPA010000042 DAHHPA010000000
VERSION     DAHHPA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.043"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            31..1692
                     /locus_tag="NBH55_001458"
     CDS             31..1692
                     /locus_tag="NBH55_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1509449.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1762..2109)
                     /locus_tag="NBH55_001459"
     CDS             complement(1762..2109)
                     /locus_tag="NBH55_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCD1509450.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2193..3134
                     /locus_tag="NBH55_001460"
     CDS             2193..3134
                     /locus_tag="NBH55_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509451.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3135..4178
                     /locus_tag="NBH55_001461"
     CDS             3135..4178
                     /locus_tag="NBH55_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509452.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4184..5254
                     /locus_tag="NBH55_001462"
     CDS             4184..5254
                     /locus_tag="NBH55_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509453.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5244..6197
                     /locus_tag="NBH55_001463"
     CDS             5244..6197
                     /locus_tag="NBH55_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1509454.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6209..7219
                     /locus_tag="NBH55_001464"
     CDS             6209..7219
                     /locus_tag="NBH55_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1509455.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7504..8325)
                     /locus_tag="NBH55_001465"
     CDS             complement(7504..8325)
                     /locus_tag="NBH55_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCD1509456.1"
                     /translation="MNRFASKEEEKLYYEHEASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8422..8700)
                     /locus_tag="NBH55_001466"
     CDS             complement(8422..8700)
                     /locus_tag="NBH55_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509457.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8968..10977
                     /gene="metG"
                     /locus_tag="NBH55_001467"
     CDS             8968..10977
                     /gene="metG"
                     /locus_tag="NBH55_001467"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCD1509458.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11456..12043)
                     /locus_tag="NBH55_001468"
     CDS             complement(11456..12043)
                     /locus_tag="NBH55_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509459.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12218..13540
                     /locus_tag="NBH55_001469"
     CDS             12218..13540
                     /locus_tag="NBH55_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1509460.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13695..14471
                     /locus_tag="NBH55_001470"
     CDS             13695..14471
                     /locus_tag="NBH55_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCD1509461.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14464..15033
                     /gene="rnmV"
                     /locus_tag="NBH55_001471"
     CDS             14464..15033
                     /gene="rnmV"
                     /locus_tag="NBH55_001471"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCD1509462.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15026..15910
                     /gene="rsmA"
                     /locus_tag="NBH55_001472"
     CDS             15026..15910
                     /gene="rsmA"
                     /locus_tag="NBH55_001472"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCD1509463.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16361..16990
                     /locus_tag="NBH55_001473"
     CDS             16361..16990
                     /locus_tag="NBH55_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509464.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17114..18223)
                     /gene="ugpC"
                     /locus_tag="NBH55_001474"
     CDS             complement(17114..18223)
                     /gene="ugpC"
                     /locus_tag="NBH55_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCD1509465.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18432..18854
                     /gene="mgsA"
                     /locus_tag="NBH55_001475"
     CDS             18432..18854
                     /gene="mgsA"
                     /locus_tag="NBH55_001475"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCD1509466.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19177..19713
                     /locus_tag="NBH55_001476"
     CDS             19177..19713
                     /locus_tag="NBH55_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCD1509467.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19753..21516)
                     /locus_tag="NBH55_001477"
     CDS             complement(19753..21516)
                     /locus_tag="NBH55_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509468.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21519..23234)
                     /locus_tag="NBH55_001478"
     CDS             complement(21519..23234)
                     /locus_tag="NBH55_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509469.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7540 a         4290 c         4771 g         6811 t
ORIGIN      
        1 tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt
       61 gcggtatgcg gccttgtact tgctggatgt tatggcggca gttcagccac aagcagtagt
      121 tcagcaaaaa gcggttcagc agatggagga ggcgtattta acttagtcgt gcctcaagaa
      181 atgcctacag ctgacttatc agttgcaaca gatacaatca gttttacagc attgaataac
      241 gtttatgaag gaatttaccg tcttgatgaa gacagcaaac cacagccggc aggagctagc
      301 gagttggcag aagtcagtga agacggatta acttacaaaa tcaaattacg agaagacgca
      361 aaatggtcaa atggtgatcc tgttacagca gcggattacg tttatggctg gcaacgaaca
      421 gtagacccag ctacagcttc tgaatatgct tacctgtatg cgccagttga aaatgcagaa
      481 gacattacag ctggaaagaa agataaatca gaactaggga tcaaagcagt tggtgactat
      541 gaattagaaa tcaaattgac gaaacaaaca ccatatttcc aatatctgct tgctttccca
      601 tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg gggatgccta tgcatctgca
      661 agtgataaag ctgtttataa tggaccattc acattagaag attttgatgg accaggaaca
      721 gatacagaat ggacttataa gaaaaatgat cattactggg acaaagatac agtaaaactt
      781 tctgaaatca aagtcagtgt agtaaaagaa tcttctactg cattgaactt attcaaagat
      841 ggccaagcag atgacgtgat cttgtctggt gagttagcac agcaaaacgc aaacgatcca
      901 gcttatactt ctgttaaaga agcgcggaca agctatatcg aattcaatca acgtgaaaag
      961 aattcaccat tcaacaatgt gaacctaaga aaagctattt cttattctat cgatcgtgaa
     1021 gctttagtaa aacaagtact aggtgacgga tcagttgtct ctacaggatt gatcccatca
     1081 gatatggtaa aaagtccaac aacgaacgaa gactttgcaa aagaagctgg aaaattagtt
     1141 tcttatgaca aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt
     1201 gattctttag aatttgattt gatggcttct gatgatgatt catcgaaaaa agtaatcgaa
     1261 tatgttcaaa actcaatcca agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt
     1321 ccattctctg tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt actaggcgga
     1381 tgggctgctg actatgcaga cccaagcagc tttactgatt tgtttgtcac aggtaattca
     1441 tacaaccgtg gtcgctggag caatgcagaa tatgacgaag cagttaaagc agcttcatca
     1501 acagacgctg gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat caagatcatc
     1561 tctgatgata tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct agtgaatgaa
     1621 aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat gggattacaa atggacatac
     1681 gttgaagaat aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg
     1741 caacctcgtt ctcatattca cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag
     1801 agcaattagt attccggcaa tgatcagcca aaagctgtac ataccgtagc tggcagacga
     1861 gagcgaggaa aattctggtt ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg
     1921 ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga taatcagaaa
     1981 cggcaatgac cataagaata gatcattaga taattgaatg agtgtaaagg aatggttgat
     2041 gaggtttttt attaaaataa ccagaatcag tagaaaagct actgcgtagg ggatagattt
     2101 ttttttcatt tgattgctcc tttatttttg gttcattatc taataatgta gcgaaacatc
     2161 tgtcagaagt caaaagctag ggggaaacat tagtgaatag ttatatcaaa tatgtattaa
     2221 aacgtgtctt tttcatgatc atcacgcttt ggttaattgc aacaatcacc tttttcttga
     2281 tgcaattgct gccgggcacg ccatatacga atcaggaacg tttgagtcct gaaacaatcg
     2341 agatgctaaa taaacaagtc ggtttggata aaccagttat cgtacaatat gggatttatc
     2401 tttctaattt gcttcaagga gattttggaa tttccttcca atttaaaaac cagccagtcg
     2461 cacatttgtt agctggaaga atcggaccat ccttgcagtt agggttacag gcaattatct
     2521 ttggaacagt cctagggacg attttaggaa cgatctctgc catgaagcaa aatacatggg
     2581 cagatacatc ttctacactc gtagcgatat taggtcgttc cattccaaac tttgtatttg
     2641 ctgttttatt acaatatatc tttgcaatca aattacaagt tttgccaatc gcaaaatggg
     2701 atggctttgt gtacacaatc ttaccaacaa ttgcgttggc catgtcgccg cttgctgact
     2761 ctgctcggtt tatccgtacg gaaatggtgg aagtactgca tagtgattat gtggaattag
     2821 caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt
     2881 tgattccatt gatgacttta ttaggaccgt tagctgtagc attgatgacc ggatcactgg
     2941 tggtagaaaa tatctttgct attcctggta tcggggaaca attcgttaag tcaatcacaa
     3001 caaatgatta tcctacgatc atggctgtaa cgatccttta ctcatttatg ctgatttttg
     3061 ttattttgat cgtggatcta ttgtacggtc tagttgatcc aagaattcgt gtttcagaag
     3121 ggagccgagg ataaatggaa atgataccaa agcgttatga aacagttgca gatatccctg
     3181 cggatgaatt tcttccatta caaaaaaata ccgaagaaga acgtgaacag attactgcac
     3241 cttctttgaa ttttatccaa gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta
     3301 tttcaatggt tttactattg gtcatcattt tgatctctgt gatcacaatc tttgtttctc
     3361 ctcatgatcc aactgcccaa aacgtggctt atatcaattt gcctcctcgt attccgggaa
     3421 tcaatatcga tggattgaat ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg
     3481 ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg aacagacggt ttgggacgtg
     3541 atgtattaag ccgcttattt atgggaatga gaatctcgtt gctgatcgca tttattgctg
     3601 cattactaga tgtaacgatt ggtgtagctt acgggttggt ttctggtttg ttaggcggac
     3661 gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc aggaattcca aacctcgttg
     3721 tcatgatttt gatgttggtc gtttttgaac caggaatttt ttcaatcgtg gctgctatgg
     3781 ccatcacaaa ctggatacca atggcacgta tcgttcgtgc acaaacaatg aaattaaaag
     3841 atcaagaata tgttttagct ggattgacat taggtgaatc aagaatgaaa attgcattca
     3901 aacatatttt gccgaatatc tctagtgtca ttattattca aatgatgttt agtattccat
     3961 ctgctatttt cttcgaagcc tttttaagct ttatcggttt aggtcttacg cctccgtcag
     4021 cttcattagg aacgatgttg agcgaagggt acaagacatt cctgtatctg ccttatctat
     4081 tatggattcc ggcagtaact ttatcggtta tcatgattgg ctttaactta cttgctgatg
     4141 gattgcgtga tgcctttgat cctaaaatga aagagtgaag acgatgacga aaattttaga
     4201 agtgaaagac ttagaaatct cttttgatac gtatgctgga aaggtacgag cgattcgcgg
     4261 tgtcaatttt catttgaata aaggagaaac attggcgatc gttggggaat ctggaagtgg
     4321 aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca agcaacgcca atatagatgc
     4381 tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa
     4441 aatccgtggt aaagaaatag cgatgatttt ccaagatcca atgacctctt tagatccgac
     4501 gatgcccatt gggaagcaag tcgctgagtc ccttagaaag cataataaag tttcaaaaaa
     4561 agagggacaa aaagctgcat tagaattgct taaattagtt ggaatacccg aagctgagaa
     4621 acgtatcaac agttatccgc atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc
     4681 gattgcactg gtatgttatc cggaaatcct gattgctgat gaacctacaa cagcgcttga
     4741 tgtgactatt caagcacaga ttttggagct gctgaaagaa attcagacaa aaatcgatac
     4801 atcaattatc tttatcactc atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc
     4861 tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt tctacaaccc
     4921 gcaacatccc tatacttggg gactgcttgg ttcaatgccg acattagata gtgcgaacga
     4981 tcgattgtat gctattcctg gttctccacc ggatttattg aacccgccaa aaggagacgc
     5041 attctatcca agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac caccattttt
     5101 tgaactatca aaaacacaca aggcagcaac ttggctactt gctccgcagg cacccaaagt
     5161 gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac aaaaacatac
     5221 ttacggtcaa ggaggaattg ctgatgccaa ataaactact tgaagttaaa ggattgaaac
     5281 agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc agctttcaca
     5341 tttatgaagg agaaacattc ggtttagttg gagaatctgg aagcggaaaa tccactactg
     5401 gccgtacgat cattcgcttg aatgaaccta ctgatggtga gatattattc gacggtcaag
     5461 atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga
     5521 tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa agtcagagat atcattgccg
     5581 aaggaattga tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg
     5641 atctattgag aactgttgga ttgaatccaa gccacggtac tcgttatcct catgaatttt
     5701 caggtgggca acgccagcgt attggtattg cacgagcatt ggcagtaaaa ccgcgtttta
     5761 tcatttgtga tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt
     5821 tgttgcaaga tctccaaaaa gaacatcaat tgacctatct atttattgca catgatttgt
     5881 cgatggttaa acatattagt gaccgaatcg gtgtaatgca taacggcttg ttattagaga
     5941 tgggaacaag tgatgaaatc tataatcacg gtgttcatcc ttatacagaa agtttgttat
     6001 ctgctattcc tctaccagat ccagaccatg agagaaaacg cagaagaatc aaatatcaac
     6061 ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct
     6121 atgcaactga gcaggaagtc ccatattacg caaaaaaact aaaacgacaa aaggaatctc
     6181 ttctggttgc taactaggag ggacatagat gctaaaagct tttaaattcc gtatgtatcc
     6241 gacagaagaa caaaaacagc aattgattcg tacttttgga tgtgctcgat ttacctataa
     6301 ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca atttttcgct
     6361 cacacccgct acattaaaaa aagaataccc attcttaaaa gaagtagaca gtcttgcttt
     6421 agcaaatgca cagcttaatt tggatcgggc ttttcgcaat tatttcaagg gacgtgccag
     6481 cttcccaaag cttaaaacga aaaaaagtat gtggcaatct tatacgacca acaaccaaac
     6541 ccgtacgatc tatctggaaa atggctactt aaagttacca aaacaaaaag aactaatcaa
     6601 gataaatcag catcggccag ttgaaggatc gattcgctcc gctacgattt ctgctcgata
     6661 taatgaagaa ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa aagaatcttt
     6721 agctaaatgg atcgggatcg cctatcatcc gaagacattg atccaaacat ctcgacctct
     6781 agaagtaact ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg cacagcggaa
     6841 attgaatgtg aaagtccgct ccgctcacca tagaaagatc aggttagatc aggcaagcaa
     6901 ttatcagaaa caaaagagaa aagtaatgga tctttatctg aaacaaaaga accagcgaga
     6961 agattatctt gaacaattat ctgggaaatt ggtcaaacaa tatgactact tatttgtaga
     7021 atcttttcca aaagaagagg cacatgctga tttttctatt cacgactggc ataaattaat
     7081 aacaaaattg cgttataagt cccaatggta taataaaaaa tttcttttga tcaataccga
     7141 cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca
     7201 ttcggttatc aagggataaa aaatgaatga aactgatagg agcgttaggg ttagccttgt
     7261 taataaaaag tcttaatgac ttttgtttct aggaattttt cttttgaaga aaagctcact
     7321 aattatgaca aaccaataaa acaatagcag aaaaagttcg tacgagcttt ttctgctatt
     7381 gttaattttt tattcagaaa gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac
     7441 tcacaaagag ttgtttttgc gttttttctt gaatgattag gtctgaatct tttgctcaca
     7501 accttattca tggtgccccg gcttgagctg atagattttt gaaggacggc cgattcctac
     7561 aggacgttca ccgatctcat cgaaatattg gagcatagct ttcttaaaat tggaatgatc
     7621 gatcgtttta taatcgatcc ctaagaattt agcaaatact tttcgagctt ccgtaatcgt
     7681 gaaatcttta cctaaaactt gtagaacttg cggctcgtgt tccattttat tgactacacg
     7741 gttgaatgct ttcaagatga tttgactatg gtcaaaagcc aaagtatctt tgccagaaga
     7801 ctctccagtg actaaatcaa gcgagatagc cacttctccg ctagataaat ggatcttgtt
     7861 actatgacgt tctagtgtga accagcgtac ttccttggca tcatctccgg cgattagcgg
     7921 ttcttctcca ataaaggcta agtagctgac tgtgacgacc cagcctcgcg gatctcgatt
     7981 aggcgtactg aatgtatgca gttgctcaat attttctttt gaaataacca caccagtttc
     8041 ttcctttgtt tctcttaaga cgctttctcc agttgactca tccttttgga caaagccacc
     8101 aggcaatgcc catgaatttc gataaggatg cccttttctt tggatcaaca aaactttcag
     8161 ctgatcttct tctttgttat agcacatcag tacgatatcg acagtcaaag agggcttgtc
     8221 atattctgga cgttcctgtt tttgatacca gttaagaaat tcttcttcac ttgcttcatg
     8281 ttcataatat aatttttctt cttctttaga agcaaaacgg ttcatctgac cacctccatt
     8341 gacagtaaaa gtacttttta ccttattgta gcagactctg aaaaaaaacc aaggatttac
     8401 ttgaaaaatc taggttatca tttaaatatg aaaaataaat ggaagtatga gattgaataa
     8461 aaagaaaagc agataaaacg gcaatacttt ataccgctct tcacgaatgg caaagaagcc
     8521 acaaataacg atatatacgt caatgataaa acgcaaaatc caagctgctg tcataaaact
     8581 gttcaaatgc ccatggaggt taaaataacc atacatacga atataaaaat agctgaatat
     8641 taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat
     8701 gattgttcca gtcccttact aagatagata gtctttatta taccacgtgt gaggggagaa
     8761 ataaaaagcc gttttttagt agatatgaaa caaaacaaaa acccgcattt attcaaccta
     8821 ttcatccttt taaaggatag attgaacgta gatagaagtc gtttatccag tctttttcat
     8881 tttgaaaaaa agctaacagc atgctttttt tatggtatac tagcaaggaa acgaattcgc
     8941 atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg aaacttttta tatcactacg
     9001 ccaatctatt atccaagcgg caaacttcat atcggcaact cgtatactac gattgcatgt
     9061 gacgcaattg ctcgttataa acggttgatg ggctttgatg tgttttatct cactggagtg
     9121 gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa
     9181 tatgtagata aaatggcggc tgatgtcaag aaattatgga aaacgcttga tatcagttat
     9241 gataaattca ttcgcacgac agatgattac cataaagcag ctgttcaaaa aatatttgat
     9301 cgtttattag aacagggaga tatttactta ggtgaatatg aaggctggta ctctgtttct
     9361 gacgaagaat tctttacaga aacacaactt gcagaagtgt acaaagatga cgaaggtaac
     9421 gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga agaatcatac
     9481 ttcttccgca tgagcaaata cgcagaccgt ctattggaat actatgaaga acatcccgaa
     9541 ttcatccagc cagaatctcg taaaaacgaa atgatcaaca actttatcaa accaggatta
     9601 gaggatcttg cagtttcacg aacaactttt acttggggag taccagtcaa gagtgatccg
     9661 aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt atattactgc actaggatat
     9721 ggctccgaag atgaaagtct attcgaaaaa tactggcctg cagatgttca gatggtcggt
     9781 aaagaaatcg ttcgctttca tacgatctat tggccaatca tgctgatggc ccttgatttg
     9841 cctctaccta aaaaagtatt tgggcatggc tggctattga tgaaagacgg aaaaatgtct
     9901 aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg tttagatgca
     9961 ctccgttatt atttgcttcg tgctgttccg tttgggtcag atggtgtctt tacgccagaa
    10021 gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt attgaatcga
    10081 acgattgcta tgatcaacaa atattgtgat ggactcgttc ctgactatgc ctcacttgtc
    10141 actccatttg atagtgaact gtctacgacg gctgctaacg ttgtcggacg ttaccatgat
    10201 gcaatggaaa aaatggaatt caatacagca ttggctgaaa tctgggtgct gatttctcgt
    10261 gcaaacaaat acattgatga aacagaacca tgggtattag caaaagacga agaaaagaaa
    10321 gctgaattga atagtgtcat gatccattta gctgaatctt tgcgtatcgc agcgatatta
    10381 cttcagccaa tcatgacaga aacgccagag aagatcttca atcaactagg attggactct
    10441 gaaacaatga atcttgaagg attacacttt ggcgaatttc cttcaggaac aaaagtagtt
    10501 gcaaaaggta caccaatctt cccacgttta gatatggaag aagaagttgc ttttattcaa
    10561 gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag tcaaatggga tccagaagaa
    10621 acagaacttg tttcaacgaa agaaaaacaa atcaaatttg atgtatttga aaaagtagaa
    10681 ctaaaagtag cagaagtcat caactgtcaa aaagtagaag gggcagataa gttgctgcaa
    10741 ttccgactag atgctggaga cagccaagac cgtcaaatct tgtcaggaat cgctgaattt
    10801 tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca
    10861 cgaaagatgc gtggacaaat cagtcaagga atgatccttt ctgcagaagc accagatggc
    10921 tctttacaag taatcgaagc accaaaatcc atgccaaatg gctcagaaat cgcatagaaa
    10981 ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa
    11041 ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta
    11101 tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata
    11161 atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt
    11221 taattgattc atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat
    11281 gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct
    11341 atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa
    11401 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
    11461 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
    11521 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
    11581 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
    11641 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
    11701 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
    11761 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
    11821 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
    11881 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
    11941 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
    12001 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
    12061 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
    12121 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
    12181 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
    12241 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
    12301 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
    12361 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
    12421 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
    12481 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
    12541 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
    12601 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
    12661 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
    12721 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
    12781 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
    12841 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
    12901 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
    12961 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
    13021 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
    13081 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
    13141 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
    13201 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
    13261 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
    13321 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
    13381 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
    13441 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
    13501 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
    13561 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
    13621 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
    13681 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
    13741 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
    13801 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
    13861 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
    13921 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
    13981 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
    14041 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
    14101 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
    14161 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
    14221 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
    14281 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
    14341 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
    14401 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
    14461 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
    14521 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
    14581 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
    14641 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
    14701 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
    14761 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
    14821 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
    14881 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
    14941 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
    15001 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
    15061 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
    15121 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
    15181 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
    15241 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
    15301 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
    15361 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
    15421 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
    15481 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
    15541 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
    15601 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
    15661 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
    15721 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
    15781 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
    15841 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
    15901 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
    15961 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
    16021 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
    16081 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
    16141 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
    16201 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
    16261 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
    16321 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
    16381 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
    16441 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
    16501 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
    16561 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
    16621 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
    16681 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
    16741 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
    16801 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
    16861 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
    16921 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
    16981 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
    17041 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
    17101 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
    17161 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
    17221 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
    17281 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
    17341 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
    17401 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
    17461 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
    17521 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
    17581 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
    17641 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
    17701 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
    17761 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
    17821 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
    17881 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
    17941 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
    18001 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
    18061 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
    18121 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
    18181 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
    18241 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
    18301 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
    18361 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
    18421 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
    18481 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
    18541 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
    18601 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
    18661 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
    18721 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
    18781 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
    18841 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
    18901 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
    18961 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
    19021 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
    19081 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
    19141 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
    19201 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
    19261 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
    19321 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
    19381 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
    19441 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
    19501 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
    19561 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
    19621 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
    19681 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
    19741 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
    19801 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
    19861 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
    19921 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
    19981 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
    20041 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
    20101 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
    20161 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
    20221 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
    20281 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
    20341 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
    20401 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
    20461 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
    20521 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
    20581 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
    20641 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
    20701 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
    20761 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
    20821 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
    20881 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
    20941 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
    21001 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
    21061 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
    21121 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
    21181 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
    21241 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
    21301 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
    21361 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    21421 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    21481 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    21541 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    21601 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    21661 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    21721 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    21781 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    21841 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    21901 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    21961 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    22021 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    22081 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    22141 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    22201 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    22261 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    22321 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    22381 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    22441 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    22501 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    22561 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    22621 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    22681 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    22741 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    22801 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    22861 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    22921 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    22981 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    23041 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    23101 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    23161 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    23221 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    23281 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    23341 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa
    23401 atgagaaaac at
//
LOCUS       DAHHPA010000043        23004 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.044, whole genome shotgun sequence.
ACCESSION   DAHHPA010000043 DAHHPA010000000
VERSION     DAHHPA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.044"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..190)
                     /locus_tag="NBH55_001479"
     CDS             complement(<1..190)
                     /locus_tag="NBH55_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1509470.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMN"
     gene            390..656
                     /locus_tag="NBH55_001480"
     CDS             390..656
                     /locus_tag="NBH55_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1509471.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            669..827
                     /locus_tag="NBH55_001481"
     CDS             669..827
                     /locus_tag="NBH55_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509472.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(824..1054)
                     /locus_tag="NBH55_001482"
     CDS             complement(824..1054)
                     /locus_tag="NBH55_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCD1509473.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            1126..1281
                     /locus_tag="NBH55_001483"
     CDS             1126..1281
                     /locus_tag="NBH55_001483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509474.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            1438..1593
                     /locus_tag="NBH55_001484"
     CDS             1438..1593
                     /locus_tag="NBH55_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509475.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1590..1814)
                     /locus_tag="NBH55_001485"
     CDS             complement(1590..1814)
                     /locus_tag="NBH55_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCD1509476.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2081..2416
                     /locus_tag="NBH55_001486"
     CDS             2081..2416
                     /locus_tag="NBH55_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509477.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2649..3590
                     /locus_tag="NBH55_001487"
     CDS             2649..3590
                     /locus_tag="NBH55_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HCD1509478.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            3592..4482
                     /locus_tag="NBH55_001488"
     CDS             3592..4482
                     /locus_tag="NBH55_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCD1509479.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4525..5325
                     /locus_tag="NBH55_001489"
     CDS             4525..5325
                     /locus_tag="NBH55_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCD1509480.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5340..6191
                     /locus_tag="NBH55_001490"
     CDS             5340..6191
                     /locus_tag="NBH55_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1509481.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6188..6505
                     /locus_tag="NBH55_001491"
     CDS             6188..6505
                     /locus_tag="NBH55_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509482.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            6498..6680
                     /locus_tag="NBH55_001492"
     CDS             6498..6680
                     /locus_tag="NBH55_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509483.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            6682..7152
                     /locus_tag="NBH55_001493"
     CDS             6682..7152
                     /locus_tag="NBH55_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509484.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            7149..7691
                     /locus_tag="NBH55_001494"
     CDS             7149..7691
                     /locus_tag="NBH55_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCD1509485.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            7688..8071
                     /locus_tag="NBH55_001495"
     CDS             7688..8071
                     /locus_tag="NBH55_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509486.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            8068..8298
                     /locus_tag="NBH55_001496"
     CDS             8068..8298
                     /locus_tag="NBH55_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509487.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            8305..8508
                     /locus_tag="NBH55_001497"
     CDS             8305..8508
                     /locus_tag="NBH55_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HCD1509488.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            8505..8801
                     /locus_tag="NBH55_001498"
     CDS             8505..8801
                     /locus_tag="NBH55_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509489.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            8878..9291
                     /locus_tag="NBH55_001499"
     CDS             8878..9291
                     /locus_tag="NBH55_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCD1509490.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            9304..9690
                     /locus_tag="NBH55_001500"
     CDS             9304..9690
                     /locus_tag="NBH55_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509491.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            10182..10254
                     /locus_tag="NBH55_001501"
     tRNA            10182..10254
                     /locus_tag="NBH55_001501"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10215..10217,aa:Lys,seq:ttt)
     gene            10640..10900
                     /locus_tag="NBH55_001502"
     CDS             10640..10900
                     /locus_tag="NBH55_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509492.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            10943..11116
                     /locus_tag="NBH55_001503"
     CDS             10943..11116
                     /locus_tag="NBH55_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509493.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            11505..12338
                     /locus_tag="NBH55_001504"
     CDS             11505..12338
                     /locus_tag="NBH55_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HCD1509494.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            12331..13746
                     /locus_tag="NBH55_001505"
     CDS             12331..13746
                     /locus_tag="NBH55_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCD1509495.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13758..15287
                     /locus_tag="NBH55_001506"
     CDS             13758..15287
                     /locus_tag="NBH55_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1509496.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            15205..16257
                     /locus_tag="NBH55_001507"
     CDS             15205..16257
                     /locus_tag="NBH55_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCD1509497.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            16259..16576
                     /locus_tag="NBH55_001508"
     CDS             16259..16576
                     /locus_tag="NBH55_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509498.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16703..17326
                     /locus_tag="NBH55_001509"
     CDS             16703..17326
                     /locus_tag="NBH55_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HCD1509499.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            17341..18231
                     /locus_tag="NBH55_001510"
     CDS             17341..18231
                     /locus_tag="NBH55_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HCD1509500.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            18254..18469
                     /locus_tag="NBH55_001511"
     CDS             18254..18469
                     /locus_tag="NBH55_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509501.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18481..18822
                     /locus_tag="NBH55_001512"
     CDS             18481..18822
                     /locus_tag="NBH55_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509502.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18822..19193
                     /locus_tag="NBH55_001513"
     CDS             18822..19193
                     /locus_tag="NBH55_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509503.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            19186..19581
                     /locus_tag="NBH55_001514"
     CDS             19186..19581
                     /locus_tag="NBH55_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCD1509504.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19583..19960
                     /locus_tag="NBH55_001515"
     CDS             19583..19960
                     /locus_tag="NBH55_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509505.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19961..20569
                     /locus_tag="NBH55_001516"
     CDS             19961..20569
                     /locus_tag="NBH55_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1509506.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20569..20910
                     /locus_tag="NBH55_001517"
     CDS             20569..20910
                     /locus_tag="NBH55_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509507.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            21152..>23004
                     /locus_tag="NBH55_001518"
     CDS             21152..>23004
                     /locus_tag="NBH55_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCD1509508.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNI"
BASE COUNT         8299 a         3364 c         4906 g         6435 t
ORIGIN      
        1 tattcatcaa atcatctata tcaacattga atatcttagc tatctcattt aaggttttta
       61 attttggtgt atattttcct ttttcccatt cgcttattga ggatgaactt tttcttccta
      121 gtttttcggc tagttcttgt tgatcaatat tatacttttc tctaagaaac tttagatttt
      181 cagcgaacat agtaacacct ccttgagtta attgtaacac cgtttcggaa aaaatgtaac
      241 aggtttctta gaaaatattt tagtgttttt aaagaagtat ttacgcaaaa tagaaatcga
      301 tttcaggaaa actgaaatat gcacttgact tcggaaaaac cgaagtatat aatttagtca
      361 gaaagttaaa gagaggaggc gaataaaata tggataccgc gaagaaagaa aaatatacat
      421 tgagacaatt aagagcttta aagggttatt caaaagaaga actgtcaagg aaaagcaaag
      481 tgacatcaag aacaatattt atttacgaaa acgatgtaga taaaatgagg aacggaaagt
      541 acgccactct agaaaaaatt gctaaggcat tgggtgttag agtttccgat atttttttag
      601 accctgattc ggaaaaaccg aaatcaataa caggtgaaac aaaacaagtc agctaggagg
      661 tgtgaatagt ggaagagaag aatataaaag taccgactct tgaagaatat acaaaaattg
      721 ttgtcgaaac agacgagcag aacccgaaaa caatcgcagt tattacggcg aatgatattg
      781 aatcatgcga aggttttcga gttcgaatga cccctagata tgattaatgt tctttatcac
      841 gtggaggtat aggatcgttt ccaaaactat cttttgattg tatttgtcca ttcttttttt
      901 ggacaataaa ctctgattgc tgttttttag aaatcttttt accatactca acagcatctg
      961 atttattatc aaatagtttt gtatcttcgg aattaccttc accacgtaca gcccatttgc
     1021 catttctagg agatacccat tgatttttac ccataacaat tcacctcgct ttcaagttaa
     1081 ttctattaag aggtgaaaag aaaacaaact gatggaggtg agattgtgag taacaagcca
     1141 aaaatcactt tgaatggcac acctaatctt caaagattag cagatgaaat ggctagaata
     1201 cgttctagac attggggagt tactgtggtg gcaaccataa ttgaacaaaa agaaaagccc
     1261 acacagccgg caagcaagta gtgggcacca tagaacaata atattatatc acacactcgc
     1321 cacttccgaa aagaggagtt gctgctctcc tttcaagttt gccaaagtcc acgtttcttc
     1381 attttggcaa ctcctcttct tggaggtggt gacggcgaga gacgaaggag ggatgacatg
     1441 aaggaagaat caaaaatagg gattttgaat gagaacaatt acacaagatt aattattgaa
     1501 acagaagaag gggaaaaagt agcagaagtt acattgaccg atgcaactcc tgctgacggt
     1561 tatcgtattc gattaactcc taaatatgat taaccttttg gtgggtgtgg atcgtttccg
     1621 tgactatcac ggctttgaat ttttccatct ctgccatgaa taaatagctc agatccttga
     1681 tttttagaaa tctctctagc gatttttact gcttctgatt ttgtattagt atgtgcagtc
     1741 gctttagaat ttccagcacc tttgacattc caaccaccat tggaattagg tacaacatgt
     1801 tgattttttc ccatagtttt cacctccatt tctatagaaa tattttatca aatagaaatg
     1861 gagaaattca ataaacgaaa ggagggatca aatgacatta caagagcatc aacacttgat
     1921 gcgaaaacta aatcaggaat accacgagga aatgaaacct agattgctag gggaacaatg
     1981 gcgtgaacgc cagaagaagt ggttagaaat aaaaaagtga ctcagccgtc caaagcaatg
     2041 agtcacaaac caaaaattat acctaaggag attttatcac atgaataaga aaattgaaaa
     2101 tcttatcgaa gaattgaaac gtgaatgcca aaagcaaggt gtttctatta tttgtacagc
     2161 ccaaaaagaa ggggaactta aaagtctcgt ttatggtgag acaacagaaa ttttactttg
     2221 tcttgctatg caagaagaac atttagatga aaatctccca ttgtcagcac atattatgcg
     2281 tagaattgct gtggatgctt acgaacaagc gaaaaatgaa gaagaaaatc aaccttcaaa
     2341 tcatactttt gttattaata acaaggaaga tttagctgat gtgatgactc gtattctcaa
     2401 gggggaattt caatgatgaa tgaaaaaaca attgatctac tcattatttt atttttcacg
     2461 ttagtgattg tagcgaaaag ttatgtaatc tttggcttgt tattcgcaat taccatgctt
     2521 gtgctttgcg tgttagcaag cagaaaagag gttaaacatg aacgagttag caaatcttga
     2581 caactattta actgatcctg attatgctaa accaccatat gaagcgccaa ttgatgagga
     2641 ggatgaagat gagtaaatct accttagaaa tgagccatca agaatggctc gaagaccgca
     2701 aaagaggtat tggtggctca gatgttgcaa ccgtacttgg attaaacaaa tacaaatctc
     2761 cttatcaatt gtggcttgag aaaacgggtc aaattgaatt gaaagattta gaaagcgaac
     2821 cagcttattg gggtaatgtt ttagaagaag tggttgctaa agagtttcag gaacgtacag
     2881 gcaaaaaagt acgcagaaga aaccaagtat ttgaacatcc attacatcca tttttaagag
     2941 caaatattga tcgggaagta gtgggagaaa atgccattct ggaatgtaaa acagccaatc
     3001 aatttctcgg aaaagagtgg gaaggtgaag aggtaccgct tagctatctc tgtcaagttc
     3061 agcattacat gaacgtttta aacaaagact attgttatat cgctgttttg atcggtggac
     3121 aaaaattcat ttggaagcgg attgaacgag atcaagagct gatcgataca atcactgaac
     3181 aattagtaga gttttgggaa acgaacgttc ttggaggtat cgagcctgta attgacggaa
     3241 gtggagcgac tgctgacttc ttaaaagaaa aatatgcaga tgtagaagaa aatcaaacag
     3301 ctctaccatc acgttttgat gaacttatcg agcaaaaaag agaactcaag cggacgaaaa
     3361 aagaaattga atcatctatt cgtcaagtag acaacgagat catcagtgag ctaggtaaac
     3421 gtgaagccag tatcggtatc acacaaagaa acattatcag ctggaaactt gttcgtacaa
     3481 gacggatgaa cacgaagaaa ctagcagaga aatatccaga tgtcgcaaat gatgaagaga
     3541 tttataacgt tactgaatca agaaggctaa ccgaaaagga gatcaaataa tatggcaaca
     3601 aatgaatcgt taaaaaatca attggcagaa aagccacaga aacaagttgc accaggacag
     3661 ttagggctta aagctctaat gaatacacca acaatgagaa agaaatttga agaagtactt
     3721 catgacaatg ctaatgcttt tatgtcgaat gttatgactc ttgtatctaa tgacagttat
     3781 cttgcagata gtgaaccgat gtctatcatg agtggtgcgt taactgctgc aacattaaat
     3841 cttgggctag ataagaattt aggttatgca tatttagttc cattcaatag taaaaacaag
     3901 caaacaggaa aatgggaaaa gaaagctcaa ttcatgcttg gctataaagg atatatccaa
     3961 ttagcccaac gatcaggtaa atacaaagca ttaaatgtga tcgaagttta cgaaggagaa
     4021 ctaaaaagct ggaaccgact gacagaagag tttgagtttg atccaaatgg tagaacgtct
     4081 gatgaagtca ttggatatgt tggctatttc gagttactga atggattcaa aaaaactgtc
     4141 tattggacca aacaagaaat cgaagctcat cggattgcta acaataaaga tcgcgataag
     4201 acaaagttaa gtggtgtgtg ggcatctgat tacaatgcaa tggcacgaaa aactgttttg
     4261 agaaatcttc tttctaaatg gggaatcttg tccatcgaaa tgcaagaagc caccacatcg
     4321 gatgagagag tccaaagggt tcaagaagac ggcagcatta ttgctgaaac agaagttgaa
     4381 gaagatattc ctgaaagaaa agaagcagag gttatatctg aagaaaatga agatgtacaa
     4441 actggattat ttgatgcatc taatccgccg ttaaacaaat aatgagggag ctttctccct
     4501 tgcttttcta gaggagaaat acgaatgaac acaggatatg tcaaattaca tcgaaaagtg
     4561 atgaattcat tcgtttggac caatccttat atgtataaat tatggagctt gtgtttgatg
     4621 aaagcgagtc atgagaatcg caagatactt tttaatggaa aagagataca agtgaacagc
     4681 ggagaattcg tcacagggcg cgatgctatc acatctgaga tgaacgaagg cgtgaaacgt
     4741 gaacatcaag tgaacagcgg ttctgtatgg agatggctaa aacaatttga aaaaaacgga
     4801 atgttgaaca tcaaatcaac tacgaaatac agcgttatat caataaaaaa ctggtctttg
     4861 taccaaagca gtgaacaaca aatgaacatc aaacgcacaa caagtgaaca acaagtgcac
     4921 acaaacaaga atgaaaagaa ttataagaat gaaaagaata ataaaacatc atcgtcgcaa
     4981 ccacgcaaaa agcgtgttta cgacaccgac tcagtttact acattctcgc ggaggagtta
     5041 ttcaaacaga tttgtcagaa tcaggaaatc aaaaagccga atctgcaaag ttgggcagat
     5101 aacattcgga aaatgatcga gatcgacaag cggaccgaaa accaggtacg gggaatgatt
     5161 gaatggagtc agcacaatgt gttttgggca tcgaatattt tatctgctaa aaaattgcga
     5221 gaaaaatacg acacaatggc agcgcaagca aatcgtgatt ataaaacaaa acaaactaaa
     5281 acgcttgaat acgagaaatt tagcacagat gagttgccta tttgagagga ggcgtaggca
     5341 tggaaactgt tggggaaatc atggaaaagc tgatacagaa agtgcttgtc caacgtggcg
     5401 aatgtcctga atgcggacag cctttgtatg gatggcgtac gaagaaccct gatggctcag
     5461 aacgttgtaa gccaacctgt atgcaatgtg ggtataaagc attgcgagtt caggaagact
     5521 tacaaacaga acgaatttac aacgagagcc tgaaagcaag agcaatcaat tttttcaaag
     5581 gtggttctgt tgtgcctaat caagcgttgt ttgattgcac attgcagaat tatcaaattg
     5641 tcgatcaaga aacaagacaa gcggttgaag taaccaaacg ctttgttaat tcagtcttgt
     5701 taggaaatcc aagtcacctt gttttaactg gaaaacaagg aacaggtaaa agtcacctag
     5761 cgatggcagc ggcttgggaa gtcttgaagc gatcaaacta cgataaaaaa atcttattta
     5821 tcgggttaca ggaaatgctg gatcaaatca aattttcgta caacaatcct gaactcagaa
     5881 aaacgattga gggatcgttg attgcagata tcaaaacagc ggatttagtc atcattgatg
     5941 atatcggttc agaactagga aaagatgcat cagatagtcg agcgtttggc ataaacacgc
     6001 taaattcgtt cttggatgca cgacagaacc tagcaacgat tatcacaacg aacttgcttg
     6061 gtgaagaact gaaaaaagct tacggtacga gaacaatatc aagaatgttt gtcaactctg
     6121 atggatttac gatggtattt tctcaaacag cagacaagcg cataaaacca gtcaaaggag
     6181 cgatcgcatg aatttgacta aacaagaaaa aagaatctgt cgtcagataa tgttgcaaat
     6241 tataggtgat cgtgacatgg acgaattatc tcaacaagag agtcgaatgg ttcggaagat
     6301 aagtaagcta atcggtgcag atacgatgga tcgatccaag ccattaccca aactgaattt
     6361 tgaatcattt acgtttgaga aatatagcca tctgagagag gtaggctata gtgtaaatgc
     6421 tattcgaaaa gcacttgggg taggaagtag tgtatgggca aaatggcgga aagaaaatgt
     6481 aaatgaggta aatcaaaatg attaagctaa taggcaaaga gacgctcaag gtttactatg
     6541 tagcggaaac aaaggcagaa gtcagccgtt ggctattgaa aatgtttaat aagactagag
     6601 ccaatgcttc agtaagtcag aaagcaagtg tatcagaaac aggcatgcct gaagcaatgt
     6661 ggatttcaag gaggaactga gatggcaatg ataccgaagt ttagagcgtg ggatagagaa
     6721 aaagacagaa tgatttatcc tagcacgaca ggagtttgtt ttgaaatgaa tgatgatggt
     6781 ataagtgtgc ttgatattga tgtagattat cccaatgact atggttttcc caaaatagct
     6841 tcaatcctca tgcaatctac cactctgaag gataagaatg gtgtggagat atttgagggg
     6901 gatatagcgc ttatcactca taaatcaacg aactacgccg atacttattg gcacagttac
     6961 gtagaagttt ttagagcaaa aaacggagcc tacagaattc gcggtgaaca catctacgaa
     7021 acagaattat atagtaatag aaaacaattg acggtagcgg ggaatgttta taattcacca
     7081 gaacttttga agagagatcg atttatgact actaccgaaa atccagagtt attgaaggag
     7141 gaacagcgat gaataaacag gaattgattg ataaatatac tgcagaaata tctagattaa
     7201 gaccttattg ccctaataga catttaatta gcgatcaact taaatatggt ctttataaag
     7261 aaatcctaga agatttaaaa caactagacg aaccgcagaa accagcggtg cctaagtttg
     7321 tggcggaatg gatagaatac gctaaaaaga aaggcgatag tctagctatt tcattcaagc
     7381 cgtggaacct ctacggtgtt gagtatagca agactgatag atggattgaa gataatcaag
     7441 gaacgtttgc tcgtgcttgg ctaattggct acgaggtcga gaaagagccg ttgtattatg
     7501 tgaagttgcc agttgtgtat tttaatcatt gggatttaga gacgtatcta atgaaagatg
     7561 atagaggaaa tataacaatt gcagacaaca acgattttga tgatatgaaa tttacggaat
     7621 cagaaataaa agcaattgat gaaagatact ggccatttgc tgtgccagtg gaagaggtgg
     7681 cggaaggatg agcaatctta aagttggaga tattgttttt tacaaaggtg acgaaggcat
     7741 catccgagaa gttatgacgg atggaaaaga aaatttttat agggtagatg tcagcagaaa
     7801 atctatactc tacctgtatg aagacgaatt agatttaggt actcaaaaac tcaacgacaa
     7861 ccagaaaatg gtgtttgatt atcttaaatc aaacgttgaa caaggtggca aatcagttat
     7921 gtattcgata ttccttctag gtgattggga ctctcgaatt ggagcagcaa gaaacgttga
     7981 tatagcttac tgtggattaa atggtaagca ggaagcagaa gtcctatcag cattcgctca
     8041 atggggatta gaacaggagg aagcggaatg aaatacgaaa taccactaag tgaagcagac
     8101 gtccagtcaa ttattaacgg tcgggaggtt aataaaaaac ttcctgatgg tactgaatta
     8161 gtcatcagac aaagttattt gaaagatatg gcagctccag tattaattga tcgttttaac
     8221 gtgactgatt ctgtggtaga gaaccattta aaagaatttc gatcaagtat agacgacact
     8281 ttcagattag ggagttgatt gacaatgaac aacagacatc gcagaataac aaaactaaga
     8341 aaacaggaac tgaatgtact aaagacaaag tttgaaaaag aatatggaat ttcagcagaa
     8401 gaaacatata aagtggcaag tcagtgtgtt gctgatgcga gtaatgctat tcgtaagttt
     8461 gggatttcga tattaaatga tgatcgtaaa tgggaggaaa tgagatgaaa ctaaaagacg
     8521 gattttacgc tagtagtcac ggcatcggcg gtttaatgct agatatgccg acaaagaatc
     8581 ctaaaacacg taagaaacca aaagtcaaag tcggtgacat ggttcgctgc gaagcagaag
     8641 ggttcatcta tccatttcgt ggatatgtag aacacgtcta taatcactca gcaatcattc
     8701 gtattgaaaa cacgatggaa tgtgataaat ggacagctaa aagcaaagag aatttagcag
     8761 tggtgagatt ggtggatatt gaactaatca atgacaaata aaaaagccgg atcgctccga
     8821 ctgattcaat aaattccaca agtttattat atcacataaa aggggcggtt tgacttgatg
     8881 caattgttac gagaggtagc tttcaaacag acaagatgta atgcgagaga tgtgctgaag
     8941 aactttcggc gtttggagcg gatggcaggt cgctctttga tagatattaa gtcgccgatt
     9001 attacggaca tgccgaaggc accgaagcac ggcaataagg cagaagacgc gatcattcag
     9061 atgatggata tagaagcaga gagagacgcg attttagcgg ctttgatggc gcttagtctg
     9121 attagtcgtc agatactcta ctacagcttt tgtgtgccag atagcttctc aaactacagg
     9181 attagccgtg aagtgggtta ttcagaaaga agcatacaac ggatgaagtc ggaagctctg
     9241 atagagtttg cagaagcata taaacatggg aaaataattg cttataaata ggaggactat
     9301 ataatgtgga aagattatgt atcattgaaa gagttgaaaa aagatcttat tttcaaaaaa
     9361 atcgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
     9421 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
     9481 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggcgatgga
     9541 catagttctt atgccgaagt cgttgtttat cataatcgaa atgagattgc taaagcggaa
     9601 tgtacagcaa acgatgggaa tggaggctac tattacagtg tctgtgcttt aaaagtcaaa
     9661 gaaaaacttt gtgtagtgac tgatgcataa aaaatggcgg ttttttggcg gaatgatggc
     9721 ggtttttagc tatttaccag tgatattatg gtagtgtcga aagattagtg ataggtctga
     9781 gacaaaataa aatgtaaggg aggaaatctc cctcatcgtt taattaagct tcgatagaca
     9841 gcagcggaaa tattaagaat aaggatgtga attttaactc cttctaaatt gttcttatta
     9901 tctatcatcc gttgctgtct attgttatta tgtcactgtg gcggaaaggg tagacgctta
     9961 aaaataaggt caatacgtcg agggatagcc ttaacgtttt atgatttgac catgcaaggt
    10021 tcgattcctt gccagcgact tagacgaaag gtagcttaaa tattaaccac atcaacatcg
    10081 tcagcgagtt gataaaggta actagcagac tctaggattt aagccagtct taccgattag
    10141 tctgttatag tagcaagctt gctagaggta gctcctctgg ttgacgtgca gctcagttgg
    10201 tagagcgcct gacttttaat caggaggtcg ccggttcgaa tccggtcacg ttaataaaga
    10261 acctacggaa acaatcaatc ttatcggatg ccgattggtt ggctgaatta agcagtgtgt
    10321 ctgtgaatca gttaataggc ataaaatact agcacagacg tgtgccactc tcaggtgtag
    10381 gttaggagag aaacattagc aaccgagggt tggaaatggg cgctcaaagt acacgagcaa
    10441 ggcgaggtcg atagtaatcg atggaatcgg tgtaggttgc tattacataa ttgattgggt
    10501 tagattgagt tttgggattc ggtacaaatg aatcgtcaaa tgactcaagc acaggatcgg
    10561 aaacgtccct gcctgtgcat tacatattag atcactcgtt gagtggtctt tttattttag
    10621 aaaagagagg attttgaaaa tgaatcatga gaagtttatc gaaaaatgca aggctattgt
    10681 acgtgaaaga attgagaatg agattgctga cctaagtgga gcagtacctg agtttagtgt
    10741 tttcatagtc tggtcatgca aaacactaca gaatagcaaa gcattagtca gcgctagctt
    10801 aaaaggagca ccgtattttg aaattacgtt gaacggggac aaaggtgaaa tctatgtaga
    10861 tacttatctc aaaaaatcaa atgaatgtat caaagtctag caggtgctag gctttttctt
    10921 tacataaagg aggaatgatc tcatgaaaca atataccgct aaagatttcg aggaaatgaa
    10981 gcgattaaag aaggactatg aagaagttga tatggagcta actgttggag tcattcaacg
    11041 aagactgcgg gtcggattag agacagcaaa ggctatttac aatgatctaa atgctattga
    11101 agagaagaat ggctaatgag gaactactgg tatatatcgc taactaatga atatcctcga
    11161 accattgatg attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct
    11221 attgtcgaaa tggggagaga agcagaacca tgtgagattg atgcgtgcaa gctcgtttat
    11281 tgcggtcatg gtttctatga tgaaccaaac attcaaaata atattaacaa gaatttgagg
    11341 gattagaatg caagaaatgg cttaaaaatc tccaatcagg acaaatcaag ttagacaaag
    11401 tttccgattt aaagatatta attgaagcag atctaatgtt gaaagatatt gaaaattaga
    11461 aaacaaaact caacctaaga agattgcgag gtggtgtgta ttgaatggca agacaacgtg
    11521 atccaagacg tgatgaagcc aaaagaattt ggttagaatc caacggagaa aagcagttaa
    11581 aggaaattgc atctgaatta aatgtttcag attctcaggt tagaaaatgg aaatcgcaag
    11641 acaaatggag cgctgaattg aaaagtaacg ttaccaatgg caaaagtaac gttactaatc
    11701 aaggtggcgc tcctattggt aatcaaaatg ctaaaggtaa caaaggaaat agccgagctt
    11761 ctccgccagt gggtaataaa aacgctttga aaacaggcga gtatgaaacc atattttttg
    11821 agacactaag tgatgaagag aaggacatct attctagtct gaatgataat ccttcttttg
    11881 ttttgtctga agaaatacgt ctacttaaga taagacaatt tcgtatgatg aagagaatcc
    11941 aacaagctga agctggacta aatgatgaag aagtcgaacg attgcagcag ctaagaaaga
    12001 ttaaaaatcc gattgaaaaa aatggtaaaa agctagaaat caagcgtgag gttatgcaag
    12061 atgtgcagat tagcagaaaa aaacatcgca aaattgatga tattctttca attgaagatt
    12121 cattgactcg gattagcaac cagttagcta aagccatcaa gcaaatgaat gaactttata
    12181 tgaatgaata cagaactgat ttaattaaag ctcagactga taagatccaa gctgagacaa
    12241 atgaaattgg cggaaataat tcaggtgaag aaatagaaga atggaaacag gcagttttaa
    12301 atgccgcaaa caaacgggcg gtgaaagaaa atgaataatg aatttattcc ttttgctgat
    12361 attggttctg ccattgatta ttactatgat aaaccagtag ctttttgcca agatattttg
    12421 catttgaatc ctgatgaatg gcaagaaaat gttttaaatg atttagctga attttcaaag
    12481 gtttctgttc gttctggtca aggagttgga aaaacagcat tagaagcagg agcaatactt
    12541 tggttcttaa cgtgtcgacc ctacgctaaa gtaatagcaa cagctccgac aatgaagcaa
    12601 ctttacgatg tactttgggc agaggtagct aaatggttaa atgatagctt gatcaaaaac
    12661 ttactgaagt ggacaaagac caaaatttat atggttggtg attcagagcg ttggtttgct
    12721 acggctagaa cagcgactaa accagaaaat atgcaaggtt ttcacgagga ccatatgttg
    12781 attgtggtag atgaagcttc tggtgtgtct gatccaatta tggaagctat tcttggtacg
    12841 ctatcaggtt ttgataataa gctgttgatg tgtggaaacc ccaataatat tgaaggtgtt
    12901 ttttacgatt cccacaattc agaccgtgat aaatacagag ttcataaagt atcaagctat
    12961 gatagtaaac gtacaaacaa agacaatata gaaatgattc ttaaaaaata tggaaaagaa
    13021 agtgatgttg ctcgtgtccg tatttttgga gaatttccca aaggtgcgtt ggattcattt
    13081 atcagtcttg aaacggttga attggctaca gaaaaacaaa ttagtgattc tttagtcaat
    13141 aaaacaacgg ttgctcatat tggtgttgac gtagctcgat atggtgatga ttctacgatt
    13201 ctctttccta gaattgctac cagggcattg gagtatgaga agtattcaaa acgtagcacc
    13261 atggaaacaa caggatatgt catcaacatg gccaagaatc taatgagtca atatccgagt
    13321 attgataaag tgatgattaa agtcgatgac actggtgtcg gaggtggtgt aaccgaccgc
    13381 ctagaagaac ttatagaaga caaacattat ccttttgagg tgtttggagt gaataacggt
    13441 tcaacatcag aagacgattt ttacgataat ttaggtactc aactatgggg aaacatcaag
    13501 gaaatgttag aagaaaatat gacagcaaat cttaacggag aacagcctgt tattgaattg
    13561 ccttctgata gttcgttaat caaagaatta agtactcgca aattcaaaat gacaagtaga
    13621 agtcgtatac gtttagaaag taaagatgat atgaaaaagc gaaatattgg tagtcccgat
    13681 attgctgacg cactggcttt agcgttttat gagccaccaa gtcactatca atttattcaa
    13741 ttttaggagg tgagcttttg attagtacag tattatcttt agagagatat aagaagctac
    13801 gtgttaaata tgcaacacaa attgaagatg gaatgtttga tccgaatggt tttatagaag
    13861 atatgaaacc attttttgct gatcgcgaaa gaaaatatct agcttatact agcgaaaaga
    13921 atgaaataga taatagacca aagcctaaca ctgatattgt aaaagttaat aataaacttc
    13981 atgctggtat gtattcaatt gtcgtagacc aagcagtcaa tcattttact ggtataccta
    14041 tcaaatggga ttacgatgta tcggaacaaa aaagaacact cattcaaaga ttaaaagata
    14101 aattcttaaa aaatgatata gaacttccga cagttccaga agcttttaac aaattaacaa
    14161 gcaatcttga ttccatgaga ttcgcaatgc ttgattctga aactgcaact tttcaaggag
    14221 cttgtggagt agctttccga ttgttagagc ctgtggaaga agatgatggt tggaagttaa
    14281 gagcaagtaa tattgaacct tggagagcag aaagatatgg aaatgccgga atctatatca
    14341 aagaaaagta tgactcttac cagaaaaaat tttttcaaga aatgaaagtt atgacaagaa
    14401 ataaaattct gacatatgct tgctatggtg atttgaattt tgttacaagc ggaacgttca
    14461 aaaaaattga tgagaccgat aaccctttgg ggacgattac cttggcagaa tttaaaaata
    14521 atacgaatcg ttattgtgat tttgaagtag ctgaggaaat tggagatgca attgatcgtg
    14581 ctttgtctga tcaacaaaac gaaatcgaac aatttaagct tgcgtacatg cttgttactg
    14641 gcacaacaat gagcaaaggc actgctaaag aaatgatgaa tcagctaggg attatcaact
    14701 taaaagaccc tactgcaaaa gctgaatatg tcacaaaaaa tttagcaaaa gatttcaatg
    14761 aatatcatat ggatctattg aaaaaacagt tttacaccat ttgtaaagca atcgatttta
    14821 acgatgaggt gtttaaatca aacagttctg gagaagctcg caagtggcaa atcattagcc
    14881 tagaagcgaa aacaaatact aaagagcaat attttagaga aggattgaaa gaatgcgcag
    14941 aaacaattgc tgcttttctt aagtttcatg acaaagtaga aattgagcct gaaaaaatta
    15001 tttttacttt ctctagatct ttgccaactg atttaagtta tttagctgaa gctttaccta
    15061 aacttgcacc atacgtatcc aaacgaacta ttcagagcca aattccattt gtaacagatg
    15121 ttgattatga aaatgaaatg atggaattgg aaagtggaag tgcttatcca gatagtgaat
    15181 acaattttgg cggaggtggc aatagtgacg atagaaacga agtattggac caaacgtcga
    15241 gaactggagg atcaagcaag gctcaaacaa gaaaatcaga cacttaaaaa attaactagt
    15301 gtatttcctg aagcactgaa agagatacaa gcaaaactat tatcacaagc tgacttacac
    15361 aatatcactt atccagaaat gatggagttt tatagtacaa gtaatcagaa aaaatatcgt
    15421 gaatatgtgg aaaaaaatta taagtcatta aaaatgtatg atgcaaaata caaagagttt
    15481 atcgatgaat tttttccacc atttgactat gcaaaagtca atcgcttatt acaaatacga
    15541 tcagatgtat ttaaaattct tgcagaatat gcgatggatg cagatgtgaa tcaatatttt
    15601 tctgatcgct tagaggaaat tcttcaaaga acatattctt ctaatgctaa tgtttttgtt
    15661 caacttttaa acgttgatat accaaattat ttaccagaaa atgaaattaa acattatttg
    15721 aactatccat ggtcaggaaa gacattttca agaagacttt ggggaaatat ttcatcgctt
    15781 gaacagaaac tctctaatgc tattgtaaaa agtgttgcta gtggagaagg cgttatacac
    15841 gcattaaaca ccatgagatt agactcagaa atttgtgaca tgtttaagtt agaagaatca
    15901 aagtataaca aagcgataga aaatctcgtt cgaacggagt atgcaaaatt tgcacaagat
    15961 ggtattgaaa aatcatattt agaaacaggt attgaggaat acaacgtatt gactgcaaaa
    16021 gatgagagag tttgccggat ttgtggagga aaggcaagta agaatcccta taaactgaaa
    16081 gatgctgtca taggtgaaaa tcgagcacct ttccatagtc gttgtagatg tacggatgtt
    16141 cctaatttac caaaattagg aaaggatatt gatgaagaat atgaccgttt atttggcgat
    16201 ttattagatg agtttgcaca tgattctttt ggaattaatt tgaaacggag gaagtagaat
    16261 gaaagatttt tttgaagcag tactaacaat taatgtaaat gctgatattg cagaagccta
    16321 caaaacagct attgagtctg agaaccatcc taatggcttg agagaccatt ggaatggcaa
    16381 ttatgcctac gtggttattg gcgatcaaac tgttaattat caagataata ctccagttga
    16441 taagaatacc gttaatttaa cgattcaatt attatctcat tcattaccaa atttaaaaga
    16501 aacagttgat tggtatgaaa agatgggatg tattgttgtt agaactgact acaaagaagg
    16561 aaagtctagt aattaggctt ttttattttg tccgaaatga cgttaaacta gcgcaatact
    16621 gggcttggtt gaatggtggg gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa
    16681 cgaatcgtgg ggcgaaagga gaatgagcat gaaacacaaa tcattaatgc caatgaattt
    16741 gcaatacttt gccgaaggtg acgatcaaaa gttttctttt gatgacttca aatcttttgt
    16801 ggaatccaat gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa
    16861 acaattggaa gcttggaaac aaaataactt agataaaatt aaacaggata ccatcaagga
    16921 atatgaggaa tctaagaaaa acaagtcacc tgagcaaatt caactggaaa aactacaagc
    16981 tgaatttgaa gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga
    17041 acaaattgct ggattagaac tagatggaga gttaaaagag tctatttctc aatttatgct
    17101 aaataatctt gttagttcgg atacagattt cactaagaat gctgttgaag gttttacaag
    17161 tgtcttgaat gcaattaatg agaaacatgc agatgcatta aaagaactac aaatgaagtc
    17221 tgcatttggt ggaactcaac aatcgaataa ccaggttcag cagaacaatg aaacattaac
    17281 aaatccagaa gaacaattag gacaaattct tcaacaattt aactaggaga gtgaaaaatt
    17341 atgaaaaaaa catctttaaa taatttagag tatttggata tttcaccagc gattaatgct
    17401 atgcaagtac caaatacacc ttttttaagc tatttatttg gtgctggaaa aacagagcca
    17461 gcaaactcga cagaaattaa atggcgtgaa tatgatatca acaacgatga ttcttctgaa
    17521 aaacttgagg gcggagaata tccagatgct gaatcaggtc gaacttggtt taacaactat
    17581 actgaaattt ttagaaaatc aacctctgta tctggtacat tagatgctat taatgtgaat
    17641 ggagtcggaa atgaattaac taatcaagta gcactacgtg gtatggaaat gaaaattgat
    17701 ttgaaccgaa aattgattac tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga
    17761 atgaatggga ttttgaactt gatcaattca gcaaataagg cagaaacagc cactgcgggt
    17821 gcagtaacaa gaaaagatat cgatacttta tttaaattga tgtatgaaaa aggttatatg
    17881 ggagaaaaac tatgcttgat ttctccagat atgcaggagt taatgactga tgagttagat
    17941 ggaaaatcaa caaaaattgt tcagttcggg gaaagagtaa cttttggatt gcaaattgga
    18001 aatatcgtgt ctaattacgg tacaggtatt gctctactag aaccatcatt gccaaaagga
    18061 acaattgccg caatcgatac taattatgtg aaactacgtc cattacgtga atggagagca
    18121 gaagaacttg caaaaacaac ggattctaga cgtatcggtc ttgtaggtga atactctctt
    18181 gaatacaatg cttcaaactc tggggcaatt ttaaatttaa agtctgaata aaagggagtt
    18241 agtactccct tttttgatag gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga
    18301 tgaagtcgtg aaatatagag taggtaaaac taaaaatttt gttgggtttg ttcatcctaa
    18361 aactcgtaga tttatcacag cagattcaaa taacgaattt atcatttcta tagatgataa
    18421 agaagcaatt gcaattttgg aagatgcaat tgatgttaat gaaatttagg aagtgatctg
    18481 atggatgaat cgctaaaaac ggaaatcatt gagtctacaa aagaagattt tccagatttg
    18541 agtgaagaac gcataactaa tttattagaa ataattttgc tagaaattga atcatacaac
    18601 acttgtaaaa atgatatttc atgggaaaag ttaaaaagcg tgattagtga agtgttgtat
    18661 aaaataataa aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg
    18721 attagttatg cttcaacaac aaatgatgtt agtgagttac ttctaggcta tggtgatttg
    18781 atacgaagag ttattggttg tggaggattg gagttttttt aatgaatgaa gcagatattt
    18841 tagaaactac ttatgaagat agttgtatta ttgaaagact gacggacatt gaagattcta
    18901 atacaaatat tactattcaa gattacaaaa aagtatatga taatcctatt tcttgcgctc
    18961 tttcacaagg tcaaatcgat ggactagcag tcatagaaga tggagagatg gtaaatgttt
    19021 caactgacac atataaatta tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa
    19081 taacaataac tcaaaaggcc agtggcttaa ttttttctct atttgctact aagccttttt
    19141 actatcctag tcattgtgaa gtgaatttga taggaagtga gaaaaatggg taatctcaaa
    19201 tatgaatcaa atgcagaaaa gatcattgag aattttaaaa atatgactgt aattgctcaa
    19261 aaagaaggaa tatccttcgt taacgattca atgaataaag tcgttagttt agctaaacca
    19321 ttaactcctg taaaatcagg taatttaagg cgaggatatc gtgtagtaaa agctagaaaa
    19381 ctatcaagcg gtcgaattgt tggagcagtt ataaacaatg aacattattt taaatatgta
    19441 gaagaaggtc ataggactaa aaatggtggc tttgtcaaag ggaaattcat gttaactcgt
    19501 gcaacgaatc ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa
    19561 atcgttaaga aaggaaagta acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt
    19621 aaaagaaatc tatcctgatg ggacaatata tcttgattca gttatgcagt caactaaaga
    19681 tttttatttc gttttatcag taatggaatc tggaactgaa aatgtaggaa ttgatattca
    19741 aaatgtttct ttcttaattg atattgcatt gattgataat aaacctaata gaaatttaat
    19801 taatgaatta gtctcacgtt gtgggacttt ttttaatacg attacaatcg atgagcaaat
    19861 attatttcca aaagcctatt tacctgatga agcagatggt gttcaacata ttcgttttac
    19921 attagaattt ccacaatata ttgaatggag tgaaagataa atgggagaaa aaagaagtaa
    19981 aaccggaatt atttctgtcg aaaaacctac ttggtttcct ttggaagatg agacagggaa
    20041 ttttccggta tatggtactg cgttgccaat gggaacagca gtaagtatca aaccaacagc
    20101 taattatgaa acaacacaag attatgggga ttcagtggtt caagatcaat ttacagcttt
    20161 tggtggtgca gaagttgaac tggaagcaaa tggatatact cataaagttt tatctacaat
    20221 tactggaggg aaaattgtta aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga
    20281 tggtgcgttt gcatatagac gtaaaaaatc gaatgggaaa taccgttata cggtctttta
    20341 taaaggacag tttgctctag attcagatga aacatcaacg attgaaggaa gtaaagtaag
    20401 ttttactcat ccaacttgga agggttcatt tgtagatgta ccaggacttg gatatatgta
    20461 ttcagttgat gaagatgatg aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa
    20521 agtagcaatt ccaattgaag aagcagaact ttcaggaggt acagaataat gtcaaaatat
    20581 caaacaacga ttaaattaac gaaaaaagat gaagaaggta aatatgagca agtacaattt
    20641 aaatctgctg aatttttacc aggaacagtt gtagaagatg ctgcaggagt tatggaagaa
    20701 atgcaaacag cgactgataa acaatcagtt aaaaaagctt taagtcgtgc ttattcattt
    20761 attgcagata ctttatttga gggacaattt actggtgaag attattgcaa aggaattgat
    20821 gctcgtgaga ttgcctcatt gacaggaaaa ttattgaagt ctgttactgc aggttttgat
    20881 gaaacttata cagagacgaa aaaaaagtaa gtgaggcgct caagtcacct tcatttaagt
    20941 attcgattac ttaccgagaa ttagatataa agacgcaatt actcgaagca ggttggacgt
    21001 taccagaaat tgaacgtact gacttggatg agttaatgcg tctttttgct tttagagatg
    21061 cggtaaaaga gcatgaagat gttgagtact atgataattt cactcaattt taggaggtga
    21121 tactttgaat aacgatgacc taattctgaa gatgatacta gatgaatctg gttttactgc
    21181 cggtatgaat aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag
    21241 tagaaatagt ggcagttcat taggtagtat ttggaaaata tttgctggaa gttttctagc
    21301 tagcggagta actagaatcg taggagctgg ctttgatcta atcaagggtt ccataagtgg
    21361 agcggttgat cgagtagata cgatgaataa cgctctacga aatttccaaa atatgggatt
    21421 tagtaattca gaaattatga agaatatcgg aaagaatggg cttttatctc aaggtattca
    21481 aggacttcct actgctttga atgatgcgat aagtcatgtt caacttcttg cttcttctac
    21541 aggtgattta actcgttcaa ctcaaatatt taaagcttta aatgatggaa ttcttggttt
    21601 cggtggttca actgatcaag ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa
    21661 cggaaaagta gacgcacaaa cttggaactc aatgatcaac gctcaacttg gacctactct
    21721 ttctgctatt gctaaaaaga tgggaattac aatgggagat ctgaaagaag gtttgtctca
    21781 aggtaagatt tctgttgaag aattccaaaa tcaattaata gaaatggata ccaaaggtgg
    21841 cggaggactt aaatcattaa gtcaaatcgc taaagattct actaagggaa ttaaaacctc
    21901 tatacaaaat gcaaaaacag ctgttacacg tggtgttggt gaggtaatag aaggattaaa
    21961 taaagcatta gtagattcag atttaggtgg atttaaggga attattgata aagtgggcag
    22021 ttcaatggaa tcgttcttaa aagtaattgc tgcaaatatt cctacagcag tatcattttt
    22081 aagtaattta tttaacgaaa ttcaaaaatt tggttctgct ttgaaattca tgatgccctt
    22141 tcttgttcct gcagctaccg cttttggagc gttcatgttt caacttaaag gtataccagc
    22201 aataataaaa agcttcaata attttaagaa tgccataatt ggcgttggaa attcattaaa
    22261 gattatgggg gcaatagctg ctgctaattc atttgtttta attattggtg cagtcgttgg
    22321 tgcaatagct gttttcggtt actttatggc aaccaatgaa gagtttagaa acaaggttat
    22381 atctatttgg aatgatgtga aagattccgt aattggtgta ttaaaaaata taaaggactg
    22441 gggaattgat acttggaatt ctgctaaaga aatggcatca aatgcagttg agggtgtcaa
    22501 agatgcttgg tcaggaacaa aggaatggtt ttctaacacc tggaaaggta ttaaggatgg
    22561 ggccacaggt ttatttgata aaacagtaga gacttcaaga aatgcagtcg atagtgtaaa
    22621 aaatgcatgg tctaatatga agaaatggtt ttctgatact tggcaaagta taaaagattc
    22681 agccagagaa aaatggaatg aaattaaagg ttctattatg tcggttgctg gtccattgat
    22741 tacaggtata aagaatgcat ttttacatgt tactttttat ctagagacct tatggaataa
    22801 tttagttgag attggtaaaa acgtctttga gattttaaaa aatgttatac ttgcgccggt
    22861 tttgtttatt acctccatga tttctggtgg atgggaagaa acaaaaaata atatgattgc
    22921 tgtttggaat aatattaaag aaagtgccat aaatatttgg gaatctataa agaatatatt
    22981 tgtaagttat tttacaaata ttta
//
LOCUS       DAHHPA010000044        22984 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.045, whole genome shotgun sequence.
ACCESSION   DAHHPA010000044 DAHHPA010000000
VERSION     DAHHPA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.045"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(105..560)
                     /locus_tag="NBH55_001519"
     CDS             complement(105..560)
                     /locus_tag="NBH55_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509509.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="NBH55_001520"
     CDS             complement(714..1922)
                     /locus_tag="NBH55_001520"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCD1509510.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="NBH55_001521"
     CDS             complement(2034..2366)
                     /locus_tag="NBH55_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD1509511.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="NBH55_001522"
     CDS             2514..3389
                     /locus_tag="NBH55_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD1509512.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="NBH55_001523"
     CDS             complement(3471..4052)
                     /locus_tag="NBH55_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCD1509513.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="NBH55_001524"
     CDS             complement(4058..5317)
                     /locus_tag="NBH55_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD1509514.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="NBH55_001525"
     CDS             complement(5319..6365)
                     /locus_tag="NBH55_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509515.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6600..6875)
                     /locus_tag="NBH55_001526"
     CDS             complement(6600..6875)
                     /locus_tag="NBH55_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCD1509516.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7118..8428)
                     /gene="der"
                     /locus_tag="NBH55_001527"
     CDS             complement(7118..8428)
                     /gene="der"
                     /locus_tag="NBH55_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCD1509517.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="NBH55_001528"
     CDS             complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="NBH55_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCD1509518.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9974..10654)
                     /locus_tag="NBH55_001529"
     CDS             complement(9974..10654)
                     /locus_tag="NBH55_001529"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCD1509519.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10703..11338)
                     /locus_tag="NBH55_001530"
     CDS             complement(10703..11338)
                     /locus_tag="NBH55_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1509520.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11404..12807)
                     /locus_tag="NBH55_001531"
     CDS             complement(11404..12807)
                     /locus_tag="NBH55_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCD1509521.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12794..13825)
                     /locus_tag="NBH55_001532"
     CDS             complement(12794..13825)
                     /locus_tag="NBH55_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1509522.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13856..14077
                     /locus_tag="NBH55_001533"
     CDS             13856..14077
                     /locus_tag="NBH55_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCD1509523.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14242..14979)
                     /locus_tag="NBH55_001534"
     CDS             complement(14242..14979)
                     /locus_tag="NBH55_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1509524.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKE
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15132..16472)
                     /locus_tag="NBH55_001535"
     CDS             complement(15132..16472)
                     /locus_tag="NBH55_001535"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCD1509525.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16709..18232)
                     /locus_tag="NBH55_001536"
     CDS             complement(16709..18232)
                     /locus_tag="NBH55_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCD1509526.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18610..19221)
                     /locus_tag="NBH55_001537"
     CDS             complement(18610..19221)
                     /locus_tag="NBH55_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1509527.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19344..19469)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19551..20267)
                     /locus_tag="NBH55_001538"
     CDS             complement(19551..20267)
                     /locus_tag="NBH55_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD1509528.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20273..20842)
                     /gene="scpB"
                     /locus_tag="NBH55_001539"
     CDS             complement(20273..20842)
                     /gene="scpB"
                     /locus_tag="NBH55_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCD1509529.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20826..21623)
                     /locus_tag="NBH55_001540"
     CDS             complement(20826..21623)
                     /locus_tag="NBH55_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCD1509530.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21593..21964)
                     /locus_tag="NBH55_001541"
     CDS             complement(21593..21964)
                     /locus_tag="NBH55_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1509531.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21983..22870)
                     /gene="xerD"
                     /locus_tag="NBH55_001542"
     CDS             complement(21983..22870)
                     /gene="xerD"
                     /locus_tag="NBH55_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCD1509532.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6443 a         4912 c         3899 g         7730 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     6481 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     6541 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     6601 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     6661 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     6721 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     6781 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     6841 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     6901 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     6961 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     7021 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     7081 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     7141 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     7201 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     7261 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     7321 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     7381 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     7441 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     7501 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     7561 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     7621 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     7681 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     7741 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     7801 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     7861 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     7921 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     7981 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     8041 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     8101 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     8161 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     8221 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     8281 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     8341 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     8401 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     8461 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     8521 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     8581 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     8641 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     8701 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     8761 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     8821 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     8881 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     8941 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     9001 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     9061 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     9121 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     9181 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     9241 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     9301 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     9361 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     9421 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     9481 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     9541 cgttttgaaa gtaggtagct gccgttttct ttatctttgc cgatcgtact gataacgact
     9601 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     9661 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     9721 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     9781 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     9841 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     9901 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     9961 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    10021 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    10081 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    10141 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    10201 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    10261 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    10321 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    10381 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    10441 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    10501 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    10561 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    10621 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    10681 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    10741 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    10801 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    10861 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    10921 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    10981 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    11041 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    11101 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    11161 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    11221 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    11281 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    11341 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    11401 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
    11461 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    11521 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    11581 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    11641 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    11701 catccatttt ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag
    11761 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    11821 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    11881 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    11941 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    12001 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    12061 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    12121 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    12181 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    12241 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    12301 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    12361 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    12421 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    12481 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    12541 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    12601 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    12661 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    12721 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    12781 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    12841 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    12901 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    12961 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    13021 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    13081 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    13141 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    13201 gttgcagata aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    13261 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    13321 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
    13381 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    13441 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    13501 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    13561 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    13621 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    13681 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    13741 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    13801 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    13861 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    13921 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    13981 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    14041 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    14101 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    14161 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    14221 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    14281 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatcttctt tttgctcacc
    14341 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    14401 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    14461 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    14521 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    14581 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    14641 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    14701 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    14761 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    14821 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    14881 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    14941 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    15001 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    15061 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    15121 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    15181 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    15241 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    15301 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    15361 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    15421 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    15481 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    15541 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    15601 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    15661 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    15721 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    15781 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    15841 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    15901 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    15961 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    16021 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    16081 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    16141 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    16201 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    16261 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    16321 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    16381 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    16441 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    16501 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    16561 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    16621 agctattgac ttgtccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    16681 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    16741 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    16801 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    16861 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    16921 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    16981 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    17041 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    17101 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    17161 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    17221 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    17281 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    17341 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    17401 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    17461 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    17521 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    17581 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    17641 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    17701 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    17761 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    17821 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    17881 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    17941 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    18001 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    18061 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    18121 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    18181 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    18241 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    18301 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    18361 cctattccct cctctttttt cagtttttta aagctttctt ctttttcatt gtagcgtttc
    18421 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    18481 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    18541 tacattattg catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct
    18601 ttttgatatt tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa
    18661 ccatggaaga agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat
    18721 caagttgaaa ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg
    18781 tgttgcgttc agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat
    18841 actcataaaa atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc
    18901 tttatgctgt ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga
    18961 taagaaacta gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg
    19021 taatactgaa cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct
    19081 gatcaatacc gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg
    19141 aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt
    19201 ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt
    19261 ttgacgtttc tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat
    19321 ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg
    19381 gaatttcacc gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg
    19441 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    19501 acatatctat tcaaaaaacg caacatctaa caattataaa aagtaagtgt tcattttgag
    19561 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg
    19621 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    19681 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    19741 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    19801 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    19861 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    19921 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    19981 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    20041 gcaacagcag aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga
    20101 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    20161 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    20221 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc
    20281 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca
    20341 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    20401 cagttgttcc atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca
    20461 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    20521 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt
    20581 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    20641 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    20701 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    20761 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    20821 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    20881 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    20941 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    21001 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    21061 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    21121 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    21181 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    21241 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    21301 aattcttctc gaggatcttc ttcccataat tcttcatcgt ctgtgccgaa ttcttggaca
    21361 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    21421 gcaacagcta gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    21481 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    21541 tcgagaggac cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    21601 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    21661 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    21721 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    21781 aaataccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    21841 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    21901 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    21961 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    22021 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    22081 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    22141 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat
    22201 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    22261 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    22321 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    22381 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    22441 cagtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    22501 caggtgcttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    22561 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    22621 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    22681 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    22741 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    22801 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    22861 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    22921 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    22981 tctt
//
LOCUS       DAHHPA010000045        22090 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.046, whole genome shotgun sequence.
ACCESSION   DAHHPA010000045 DAHHPA010000000
VERSION     DAHHPA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.046"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(109..627)
                     /locus_tag="NBH55_001543"
     CDS             complement(109..627)
                     /locus_tag="NBH55_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD1509533.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(629..1246)
                     /locus_tag="NBH55_001544"
     CDS             complement(629..1246)
                     /locus_tag="NBH55_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCD1509534.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1243..2097)
                     /locus_tag="NBH55_001545"
     CDS             complement(1243..2097)
                     /locus_tag="NBH55_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD1509535.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2094..3005)
                     /locus_tag="NBH55_001546"
     CDS             complement(2094..3005)
                     /locus_tag="NBH55_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1509536.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3046..4380)
                     /locus_tag="NBH55_001547"
     CDS             complement(3046..4380)
                     /locus_tag="NBH55_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1509537.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4588..5418
                     /locus_tag="NBH55_001548"
     CDS             4588..5418
                     /locus_tag="NBH55_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCD1509538.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5662..5944)
                     /locus_tag="NBH55_001549"
                     /pseudo
     CDS             complement(5662..5944)
                     /locus_tag="NBH55_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6102..6296)
                     /locus_tag="NBH55_001550"
     CDS             complement(6102..6296)
                     /locus_tag="NBH55_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509539.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6801..6986)
                     /locus_tag="NBH55_001551"
     CDS             complement(6801..6986)
                     /locus_tag="NBH55_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509540.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7836..8177)
                     /locus_tag="NBH55_001552"
     CDS             complement(7836..8177)
                     /locus_tag="NBH55_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD1509541.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8177..8407)
                     /locus_tag="NBH55_001553"
     CDS             complement(8177..8407)
                     /locus_tag="NBH55_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD1509542.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8694..9032
                     /locus_tag="NBH55_001554"
     CDS             8694..9032
                     /locus_tag="NBH55_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509543.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9237..9812)
                     /locus_tag="NBH55_001555"
     CDS             complement(9237..9812)
                     /locus_tag="NBH55_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCD1509544.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(10421..11002)
                     /locus_tag="NBH55_001556"
     CDS             complement(10421..11002)
                     /locus_tag="NBH55_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1509545.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11185..11811)
                     /locus_tag="NBH55_001557"
     CDS             complement(11185..11811)
                     /locus_tag="NBH55_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1509546.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11833..13164)
                     /locus_tag="NBH55_001558"
     CDS             complement(11833..13164)
                     /locus_tag="NBH55_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCD1509547.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13319..13720)
                     /locus_tag="NBH55_001559"
     CDS             complement(13319..13720)
                     /locus_tag="NBH55_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCD1509548.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13806..14012)
                     /locus_tag="NBH55_001560"
     CDS             complement(13806..14012)
                     /locus_tag="NBH55_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509549.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14107..15609)
                     /locus_tag="NBH55_001561"
     CDS             complement(14107..15609)
                     /locus_tag="NBH55_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCD1509550.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15627..16082)
                     /gene="argR"
                     /locus_tag="NBH55_001562"
     CDS             complement(15627..16082)
                     /gene="argR"
                     /locus_tag="NBH55_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCD1509551.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16180..16797)
                     /locus_tag="NBH55_001563"
     CDS             complement(16180..16797)
                     /locus_tag="NBH55_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCD1509552.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17018..18391
                     /locus_tag="NBH55_001564"
     CDS             17018..18391
                     /locus_tag="NBH55_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1509553.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18466..19578
                     /locus_tag="NBH55_001565"
     CDS             18466..19578
                     /locus_tag="NBH55_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCD1509554.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(19650..21287)
                     /locus_tag="NBH55_001566"
     CDS             complement(19650..21287)
                     /locus_tag="NBH55_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCD1509555.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(21455..22063)
                     /locus_tag="NBH55_001567"
     CDS             complement(21455..22063)
                     /locus_tag="NBH55_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1509556.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6439 a         4501 c         3857 g         7293 t
ORIGIN      
        1 aaacttaagt tattaagaca aatgaataaa caactacgct ttcctcgaaa caaaaaaata
       61 aagcacaaat gcgcatttac gcactcatgc tttattcgta caattaattc agataacggc
      121 agcgcatagg atgccaataa cacttcctaa aatagctcct cctaaaatgt cagtaggata
      181 atgtaaaagt aaaaaaatcc tcgaataagc gatcaaacaa gcaaataaaa aggctaatat
      241 tcctgcaact ggacttaatt cgaacaacgc agctactgat aaaaaggcta gcgctgtatg
      301 tcctgaagga aaagaagaag tcttcggttt acttgtgtaa aggacaagcc gacgattttt
      361 ttcataaggt cttttccgtc ttgtcgtttt tttgataaaa agcaaaccaa caaaaaaata
      421 gatcaaactg ccgataagaa tggctagacc atattctcta gtatcaggta tcaagactaa
      481 aacagcagca aggagtaacc aaataaaacc accattagag atattactga taaatttcaa
      541 aaaattgatt tttcgactat ccgttttccg ttgcttggcg aacttgttga taatactatc
      601 atcgatttta gcaatcaaac gcaacatgtt attcctcctt tttaagaacc ttttcttgcg
      661 tacgatggat aaaaagcagg acttgaaaat aaaacaagaa actgaaacct aataaagcaa
      721 aatagattgg tcctgcaaac cagaatgcga caagaaggat caacgtgcct gccaaataga
      781 aaagcgcgct tttgaaatca ttaaaaaaca agaaaaatgc agtaatccaa gaagcttctt
      841 tttgttccag ttgaagcaag attttgtaac taaagagaag cgcgcttaat agaagcaaaa
      901 acaacgtagt atacatcaat gtatagccaa ttcccccacc ccaatagcgg aaaatctgat
      961 aatcgatcaa tagaagaaga aaataaacac ttgctaaact atcttgcttt ccataaagag
     1021 agaagttctc acgaaacttt ttccagaata gtgaaccgag ctttttctct tcattttttt
     1081 tgtaatgata aatcatatac agaactgttc gacttgcagt tcctaatgtg aaaacaggca
     1141 agacgcacag gaaccatagt agctgtaaat ggataagacg tggaatccat tcaatgattt
     1201 ctaagaaata atcattggct tttttaccaa tcgatcgatt catcatcctt ttgaacctcc
     1261 tgtcaaatcc atcccttcaa tgaaataacg ttgtgcaaaa aagaacagca aaatcgtcgg
     1321 gatcaagaca agtgttgctc ctgccatcag ataattccat tgtgtcgttg cttggttctg
     1381 aaaaacttgc aagccaatct gtaacgtgta tttttcttga tcttggatat ataacagagg
     1441 acctaagaaa tcattccaag ctgcgttgaa ggaattgatc gcaatggtaa tcaaggcagg
     1501 tttcgtcaat ggaagcatca aatgactcca aatatacaaa tgattcgctc cgtcgatttc
     1561 agcagcttcg atcaattcat tattgatcga taagtaaaat tgacgcatca gaaatatatt
     1621 gaacgcattg ccgaagaacg atggaacgat caggggcaaa taagtaccta cccagccgat
     1681 cttggagaat aatacatatt gagggatcat cgttacaaac cccgggatca tcatcgtaga
     1741 aagaaccaaa gcaaacatca gctttttccc aggaaaatca agcttcgcaa atccataagc
     1801 aataaaagaa tttgaaagga cattaccgat cactactagc acagtgatga ataatgtatt
     1861 tcttgcatag cgccaaaaag gaaaggcggc aatcgtatcc agatagtttt cccatacgat
     1921 tttttctggg aaaaaggtcg gaggataacg aacgatttct tccattggtt tcaatgaagt
     1981 aaacaccatc caaagcaatg gaatgatcaa aatgattcct attactgtca tgaggacata
     2041 acttagtatt ttttttgttt gccgattcac actggtcttt ttgaaataat ccatcatttg
     2101 ccgtcacctc cagaataata gacccactta ggtgcaagct tcaaattgac aaacgtcaga
     2161 accaacacga tcagaaacag gatcattgac atagccatcg cataacccat atcaaaagct
     2221 acgaacgcct tgttccacat atgcagatta taaaaaagca aagaatttgc aggtccgcct
     2281 gttccatttt tcgtcatgac ataagcttct tgaaaaattt ggaaagaacc gatcgtagag
     2341 gtgatcacat cgtaaaaaat aataggagtg attaatggca aagtaatact tttgaacttt
     2401 ccccaaacgc ctgctccatc gatttctgct gattcatata aagatctagg aacattttgt
     2461 aatgtagcta agtaaagtag cattgctcct ccagaactcc atattttcat caaaatcaac
     2521 gcaggtttcg tccattgtgg atcaaacagc caagctggtc cagggatatg gaaccagccc
     2581 agaaatgtgt tgatcaatcc tgcagatgga gaaagcagct gcatccataa gatgtagact
     2641 gctactcctg ataagactgc cggtaaatag aaagctgtac ggaatacact gattcctttg
     2701 attttttgat tgagtactgt ggctaaaaac acactgaaaa tcgtcgtcag cggtacattg
     2761 aagatgacga aataaacggt gttgtataac gatttccaaa ataaatcatc gtgaacgaac
     2821 attcttttga aatttgctag accaatgaaa ttcatcttgg atgtcaaatt ataatcggtg
     2881 aaactaccaa taactgaaaa aagcataggc cctagcataa ataccaagaa cccgataata
     2941 aaaggcgaga taaataaata ggcccatctt gcttcttttt tctttcggat agataaacgc
     3001 ttcaaatttt cttgcctcct ctgtacctag acagtcaaaa atggattatt gattttgctt
     3061 gactaagttt tctactgctt tttgtgcatc tgccaatcct tcttttggtg attttgttcc
     3121 taaaagaatc tggtcaatct gttcattcac taatgtcgtg taatccggag catctaaagg
     3181 aactggtgtg atcaccgtat ttttaaagtt ttcgtccgcc atttgataga tcatttttcc
     3241 tttatccgtt aggttgtcat tattcactaa attctcattg gctgtcttgc tcgccatgat
     3301 atcaaaactt ttttcgccga atttttcttg tacttctgga gaagaaagat atttgatgaa
     3361 ttcataagag gcctcaggat cttttgctcc gtatggtact tccaagacga atccgccacc
     3421 ccacgaccaa tggcctgaac cttcttcttt ttctgggagc tgcatcacgc caaaatcaaa
     3481 atcatccggt gcattttcca tcaaactaga gtaataattg atattttggg cacgcatggc
     3541 tactaatccg gaaataaatg gatcagatac accagaccca aattcggctt ctaatttatt
     3601 gatcgtattt ttcccataat aatcttgcca gtcttggatc cactccaagg cttccacttt
     3661 atttttagta tcaatcttga ctttttcatc tttgtcaaac caactagtcc cgttatctgc
     3721 gtttagcgcc catacatcag cccctaaatt ccataatgga taaaaaccga tgcgtgtaaa
     3781 ttcttttcct tcttttttat ctagtttacg tgctgtttct tctaattcat cccaagttgt
     3841 cggtagatct tgttcactga ttccagcttc tttaaataac tttttattat aaaaaatgac
     3901 ttgtgtatat gtattgaatg ggatcgcata aggatcacct tcatattcta ctgtatccca
     3961 aagctgcgga taaaaatcct tagaagtgtc tttttctaca tatttggaga gattagttgc
     4021 ttgctgtgct tctgctcttt gggcaacgga atttatgtcc tgtacaataa catcgggtgc
     4081 atttccagct gttattgcag ataaagattt agtccagatg tcaccccatg gctgatactt
     4141 ataatttaca tggatctcgt tttgtgattg attgaaatcc tcaatgattt cttcgatagc
     4201 ttcttttcgt gcaccggatc cccaaaagga ccagaaatca atattttttc ttccatcact
     4261 tgttttttca gaagaatttg ctgcacttga actggcatcg cttgcattac ttgatccgca
     4321 tcctcctaat aaaaacaatg ctgaacttgc cagcaaccct gtaaacaata attttttcat
     4381 attttacctc caaattttaa tgtaaccgat gtcggtttta atataacgat atcattttgt
     4441 tctgaaaata acaatttata aggtatgatt ctaaaaaaat tattaatgat accaatactt
     4501 tatataattc gcaagcgttt tctttgaatt ttttaatctt tcgtagcaga atgagaatgt
     4561 attaaaaata gattaggagg aattttgatg tttagacacc agaaagaatt gcagtttgaa
     4621 gcaaaaccag atcgtccgaa tccattaatc gcgaaatatc tgcaggagct gatcggcggc
     4681 caatatggcg agatgagtgt agctatgcag tacctgttcc aaggctggag ctgccgagga
     4741 gaagaaaaat ataaagatat gctgatggac attgcaacag aagaaatcgg ccacgtggaa
     4801 atgttggcga caatggttgc tcgtttatta gagggtgcgc ctgttgaaga tcaagaagca
     4861 gcgatgcaaa aagatccaac aatcggtgcc attatctctg gaatgaatcc acagcacgcg
     4921 atcgtagctg gcttaggacc tcgtcctgct gacagcgtgg gcaatccatg gagcggtgcc
     4981 tatatcattg cgagtggcaa cttgttagca gactttagag ctaatttaaa tgccgaatca
     5041 caaggaagac ttcaagtagc acgtatttac gaaatgatcg atgatcgtgg cgtaaaagat
     5101 atgctgtctg ttctccttgc aagagatagc tatcatcaaa atctatggga atgtgcagtg
     5161 aaagaattag aagaaaaaga aggttcaatc ttagtcccaa gtactttccc gcgtgacaaa
     5221 gaacgaaaag acattgctta tgacttctat aacttctcag agggagacca aagtagtgaa
     5281 ggttcttggg ctagtggcaa agcactggat ggcgaaggcg aatatcaatg gttgaaagaa
     5341 cctaagatcg aaggcgctgc accaaaatta gaaccagtaa caccaaaaat gtatgggaca
     5401 cctccattca agaaataaat atctacccgt agaaacaccc gttttttgtt ccacacgctt
     5461 tgtgagcaaa actgactaaa gtcattaatc ataaccaccc ttcaaaaggg tggttttctc
     5521 tagagctata agctcaaatc accggccagc gcctaaagac gctggctttc atattttatg
     5581 ttcaagccat attgcctttg ccagttttgc ccacctctta aaaggtttat gtactactcc
     5641 ttagccctta aaaggatcgt cttactctcg tacactcagc ttatctaacg cactatcatg
     5701 tttttcttgt tcttgaatgt actttttgat ggtcgcttca ttttgtccta cttttgtcac
     5761 atagtatcct tccacccaaa agtgtcgata cccaaattta tattttagat ttgcacaatc
     5821 tcttgatgat ctcctgaata ttggctcgat attgattata aattattttt cttctatact
     5881 ttggtgtgaa tacaatgtgg tatttacaca tctactttgt acgggctaaa ctatttccct
     5941 ttattgctcc acccgcatag caagtggttt tgtatttcaa tcgctcggcg cgctcaatta
     6001 ggtctaaaga caataaatct aaaaacccgc tgcttcactg acagaaacaa cgggttttca
     6061 gaaaaaaatg aaatgaaaaa gatctcttac ggcaatctat tttatttttt tgatttttta
     6121 taaatggaaa atgcttcaag aagtccaaga aataaagccg tataaaaacc agaacctatg
     6181 aaatctttat ttattagata ttcaacagaa atcccgataa cactagccaa caatatattt
     6241 atcagaagtg taaaataagg atgcttttta tagaatagaa ctagtgttgt tttcatagac
     6301 tctcctttct tcatatttcc aattaatcac aaattcacgg cattactaga aaaattaaag
     6361 gggcgcagaa gtggaaaaat actcttcgac tttccctaca aaccaggcca tcttgtgttt
     6421 aataactata ccaaatctaa ggtacctcta aatctaaatt ccatttgtgg cggtgacctc
     6481 caatttttaa ctcttctatc tcccatccag tatacataac cttgatactt cgaccactta
     6541 tatatcgaac acacaaatac ctatccattg caggagtact acctaactta gttgctactt
     6601 gcatctaaat aacagctgaa aagataggac ttactcttag taaagtatct gaactattaa
     6661 tttttaaact atcattttta tcctcaacta catgcacatc tgttgcaact tcactttctg
     6721 tctcatccag ataaatgatt aaggaatcgg gaattgcaat atcttcagct gacgctggaa
     6781 tagttgagct aaattagtca tcatatcttt attataaaat aaccaacagc aaaaaataca
     6841 atgaaaaaca aacttggaac taatgtatca ttaataaatg ttgaaaaatt cagcaactca
     6901 ttactgtatg attttagcag cccaaccaac atatacccta taaaagctaa taagatggtt
     6961 aggaatcctt tagaattttt actcattcat agacacttcc ttaaataact tttattatag
     7021 aaggtattgt aaataataat attgacaaaa agtgactcaa aataaaaaaa accttacatt
     7081 atactggaat gaactgtatt gatagtgtac ctaaaagctc tactgacata agatcgtgta
     7141 atcggtaaag taattttatt aattatacgg gatgtaatca attagagaat aaactaatca
     7201 taaaaggtac ttttacattc ataattgtcg ctttgagatt ctactttggt tcactatctg
     7261 acccaattgc acattttcga tcattacaag tattggtgtc gttagaacaa ctaaataaaa
     7321 actagcggta gatttttcag aatctatgct aatttctgta tggatgttcc agtgatggca
     7381 aatttctctt ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa
     7441 actatagaaa ttctttccta gacagatctt gatcccaaat tttacgtttt tgagcatcga
     7501 tatagtttaa aatgacatta ctcatatcta aagagtttag attttttagg actaaaactg
     7561 tcagttgttc tttttcttct ttctgttaaa gtattgtcgc gacttttcgt atacgttaag
     7621 atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc
     7681 tcaatcattc gtttaaaatg ctatatattc tttagtttaa gatacggaaa ttaatgccat
     7741 cgaccacact agtagaatga acgacattta cttggccttt ttttaatttc gttatgttta
     7801 gcatataaaa atattataga gtttacttac aatgcttaaa acatatactg tataatctga
     7861 tcaattttag ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta
     7921 aaatcaagag attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt
     7981 aatgagtaac gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac
     8041 attgttttac ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt
     8101 tcttttccta cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa
     8161 attctttctt ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt
     8221 ctttctcata atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta
     8281 acgtaattgt tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct
     8341 ttccgttatc agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa
     8401 caagcattat catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat
     8461 tcccctttgt aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa
     8521 tagaaataaa ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat
     8581 tgaacctatg gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt
     8641 ttgcagataa aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg
     8701 attataaaca aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca
     8761 cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag
     8821 aaacattggt tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg
     8881 tagctatttt tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat
     8941 ggcaacctct tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga
     9001 attttagatt ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa
     9061 aagactcgca gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga
     9121 ccctatagat gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga
     9181 tctgtgctga agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat
     9241 accttctcca ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata
     9301 tctgttaccc aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt
     9361 ggtgcgactg tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc
     9421 tgatggctac gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat
     9481 ttgtcttttt cttcattcca ctcataaaag ccactcattg ggaaaacaca acgattttgc
     9541 aaaaatggtt gatgaaaggt ctttttttct tctactgtct ctgcacgggc attaatcatc
     9601 aattgtcctt ttttaaatcc ggtaaatccc cacttcatga tttccggagt gaggatgttt
     9661 tcttcctctt ttcttaacgt cacaacaaga tttgacggaa aaacttcccc agctgctaat
     9721 tctgctgcct gcttcggttg tttcgtctgg gcttctcgat aataattctg aaggagctct
     9781 gactctagat caaaaaaata acgaccacac atcctaatcc ctcccaatct ttttttctat
     9841 catacactgc aacaagaagt tcgcaccaac aaaacgcttt caatttttgt agaactaaaa
     9901 aagttgtgac aagtgacaaa ctctccagtg aatggtattc tcctgccaac tcctcggtca
     9961 caagtcacaa tattcaacaa acatgagaag ctgctttttg aaattgttcc ttgtgactcg
    10021 gagtgaaacg gcaggcttac gacctctggg taaacggtgt tcaaaagctt gcatctgcgg
    10081 taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg ttcttattac
    10141 ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc agctaacctt
    10201 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat
    10261 actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga agtagcttct
    10321 tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
    10381 atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc ttttgcgaag
    10441 ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga tcctgtgtga
    10501 ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct tgacggtctg
    10561 cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc gtatacttga
    10621 cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact gtactcaact
    10681 caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca tgatcagcga
    10741 aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca tttaagatgt
    10801 cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct agacaaactt
    10861 ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa gctgctagaa
    10921 tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg gtcgcttcca
    10981 agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag tcctatatta
    11041 tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc caaattatct
    11101 gacacagccc tcactataaa actgttctca tatagctaga tgttcattga ttcaagaact
    11161 gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct ctgttgtcat
    11221 cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt cttggccagg
    11281 agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctatca attcacgcat
    11341 atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt cgattccatt
    11401 ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat gaggagagct
    11461 gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg ctcctgaacc
    11521 ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa gcatcatatg
    11581 ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat gaggagaaat
    11641 aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat tttcgatcgt
    11701 attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg gtttcaagaa
    11761 ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca tggtggattc
    11821 tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg gatgagaata
    11881 gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg aaatcaagtt
    11941 gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt cagtttcagg
    12001 gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt tagggacacc
    12061 gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt gtttttctgt
    12121 taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag ctttccggtt
    12181 attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat aatcatctag
    12241 agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa tgttgtctgc
    12301 atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt taacatctgt
    12361 attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca aaacttttga
    12421 cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg ttgattcttg
    12481 gtctgctact tgatctacag atacacctag atggaattcg attcccgcat ttgtcatgtc
    12541 ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag aatgggcatc
    12601 aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca agccaatata
    12661 tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg cttgcgtaga
    12721 atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg gttcagaacc
    12781 agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat tcgtcacttc
    12841 gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag ttgcctcatc
    12901 gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt tccgagcaac
    12961 agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag aaggaataca
    13021 gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga ctaaaaccga
    13081 ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa aaccgatcac
    13141 aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac cgattggtac
    13201 aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa
    13261 aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct ttattcttct
    13321 aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa gggcagaatt
    13381 catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg atatgcactt
    13441 ctaatactac accgatctga tagccgccat catctaaatt ataaagagag acacgtgctt
    13501 ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc gccccattga
    13561 aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct gcatggattc
    13621 ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct ccattgcgcc
    13681 cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc ctttcgattg
    13741 accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt ctacaaaaat
    13801 tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa caaaaaaata
    13861 ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt ttctacataa
    13921 tcttttatat gattgatttt aagatacata tataccagaa agttgacccc ataaaccaat
    13981 actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg gtctccctac
    14041 cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc tttcaaaaag
    14101 actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata attttgctaa
    14161 ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta atactagtaa
    14221 cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct cttctagatc
    14281 atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt cttccaacag
    14341 tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt
    14401 taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt catctaatgt
    14461 gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga catatgcacc
    14521 atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga tggctagctg
    14581 tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc gatagccgga
    14641 aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat gagaagaagt
    14701 aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg tacttaatac
    14761 tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg cagaacgatc
    14821 cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag cctgcttgat
    14881 actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc ggccatctgg
    14941 ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt tttcagcaaa
    15001 gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta gtggtttgct
    15061 ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa
    15121 aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct gctgtgatag
    15181 gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt ttattccatt
    15241 ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg agaactgttc
    15301 aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga tgacatgtat
    15361 cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc ttatcctact
    15421 gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct tctttttctt
    15481 tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca agtttacttc
    15541 atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca gcgctttcat
    15601 atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt ttctgcaaat
    15661 cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc ggccatcgtt
    15721 ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc ggcaccattt
    15781 cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac ctcgcgtcta
    15841 attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa taatgtatag
    15901 cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat gtctcgagaa
    15961 atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt
    16021 gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg tttctttttc
    16081 atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc ttcatatttt
    16141 ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt ctattttaac
    16201 acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga tgtaaatctc
    16261 tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt ctaatttctc
    16321 aaacgttgca ggttcttcgg catagggccc gacgtgtaag atttgggcac aaagaccttc
    16381 ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc tagaacgctg
    16441 aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca tcaattgata
    16501 agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca gtccccattg
    16561 tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat tttttttagg
    16621 cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt taaagtcttc
    16681 ctgattcggg tcgccgatcc ctctgatgct aaagaatggt tttttaggaa cctctagtat
    16741 ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt tttccatttt
    16801 tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg agtgacttgg
    16861 ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa ataaatcgta
    16921 cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata atagaaatca
    16981 ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa aacagctgct
    17041 tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat tgatcgagac
    17101 ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga ctgcacaagt
    17161 ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc tgtctactta
    17221 tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc ttttcttggc
    17281 aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg gtcgattgct
    17341 ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct tgacatttgc
    17401 ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg ccattgctcc
    17461 agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga atcatatctt
    17521 cctgtttctt gtgcccgttt tgattctttc tttgattatc ggctggtttg ctattcccga
    17581 gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa tttgtctttt
    17641 gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct ggattgcttt
    17701 aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag tggagcatcc
    17761 actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga ttggcttctt
    17821 agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc ttcagatcgt
    17881 tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag cggttggtgc
    17941 tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga agccaatttt
    18001 tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca taggacacgc
    18061 ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg gattatctta
    18121 cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag gagacgggaa
    18181 cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat tagtagcggt
    18241 cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta ctacattagg
    18301 atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg ctcgattcgc
    18361 ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg ttgcatctgc
    18421 cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga aaaaattgcc
    18481 attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac atcaaaattc
    18541 aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc tgggattatc
    18601 tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa agacggcgct
    18661 gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta cgcacaatca
    18721 gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct tgccgagaaa
    18781 cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga acagacatct
    18841 caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg tggtcgtctg
    18901 gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca aatggagcag
    18961 atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa gggaccgttt
    19021 ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa gcctttatta
    19081 caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt agcatttcag
    19141 acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga agctgatttc
    19201 attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga tcaaggctgg
    19261 gatttggctc ctactagcga agcttataag ggattgacta agagtacaga acagttcttg
    19321 actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac ccgtgcttat
    19381 acgtcagatt tttccccatt ttttggcgta ctgccagatg atccaagctt actggctgct
    19441 agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct attagccaac
    19501 ttcttaaata ctggtcattg gtctacggaa gattatcaaa aggacatctc cacctatatt
    19561 acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc gatattgcgc
    19621 atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa attctccaaa
    19681 ctttcttttg cttcatctag caagagatct tttcctatct gagcttcaat gaatcgtaaa
    19741 cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa agaaacagga
    19801 aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt gaattctgcc
    19861 gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat ttttttatca
    19921 tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa caactctcga
    19981 aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa tggttcaatg
    20041 actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg ttttgttgct
    20101 aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat tggcggaagc
    20161 gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg atcgactcgg
    20221 tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat atctcctgtt
    20281 actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca agtaaggaaa
    20341 gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta agaaggttcc
    20401 aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg caagatcgaa
    20461 acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga accggacaac
    20521 ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat cccaccgtgg
    20581 caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt
    20641 aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc atcaaacaaa
    20701 atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat ataaagatct
    20761 ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt cgctggctga
    20821 aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag caaatgactt
    20881 tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa ctggcgcttg
    20941 cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag
    21001 tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc attgaatact
    21061 agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt taaatctgtg
    21121 atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctgtc aaaaacttca
    21181 tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga taacagtgga
    21241 tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc tcctcctcat
    21301 tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat ttgtactgct
    21361 gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact tgaataaaca
    21421 agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt gctaaccaga
    21481 atgttttagc agcatcccat gaaccataac gagatgttac atattgttct gctacacgtt
    21541 cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga taacggccga
    21601 tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg atccattctt
    21661 ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct acttcaactg
    21721 tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta gatgctgtat
    21781 tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg attgttaatt
    21841 gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca attgcatcga
    21901 tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca cctgatttta
    21961 ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca gtagcaccag
    22021 cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac tccttttttt
    22081 ctccgttttt
//
LOCUS       DAHHPA010000046        21990 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.047, whole genome shotgun sequence.
ACCESSION   DAHHPA010000046 DAHHPA010000000
VERSION     DAHHPA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.047"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(24..392)
                     /locus_tag="NBH55_001568"
     CDS             complement(24..392)
                     /locus_tag="NBH55_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCD1509557.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(1003..2394)
                     /locus_tag="NBH55_001569"
     CDS             complement(1003..2394)
                     /locus_tag="NBH55_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCD1509558.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2607..2993)
                     /locus_tag="NBH55_001570"
     CDS             complement(2607..2993)
                     /locus_tag="NBH55_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCD1509559.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(3049..3672)
                     /locus_tag="NBH55_001571"
     CDS             complement(3049..3672)
                     /locus_tag="NBH55_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCD1509560.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3736..4404)
                     /locus_tag="NBH55_001572"
     CDS             complement(3736..4404)
                     /locus_tag="NBH55_001572"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCD1509561.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4416..4856)
                     /locus_tag="NBH55_001573"
     CDS             complement(4416..4856)
                     /locus_tag="NBH55_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509562.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4915..5586)
                     /locus_tag="NBH55_001574"
     CDS             complement(4915..5586)
                     /locus_tag="NBH55_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1509563.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(5771..6169)
                     /locus_tag="NBH55_001575"
     CDS             complement(5771..6169)
                     /locus_tag="NBH55_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCD1509564.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(6166..6939)
                     /locus_tag="NBH55_001576"
     CDS             complement(6166..6939)
                     /locus_tag="NBH55_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCD1509565.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6936..7619)
                     /locus_tag="NBH55_001577"
     CDS             complement(6936..7619)
                     /locus_tag="NBH55_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCD1509566.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7786..8673)
                     /locus_tag="NBH55_001578"
     CDS             complement(7786..8673)
                     /locus_tag="NBH55_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD1509567.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8670..9539)
                     /locus_tag="NBH55_001579"
     CDS             complement(8670..9539)
                     /locus_tag="NBH55_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1509568.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9676..10962)
                     /locus_tag="NBH55_001580"
     CDS             complement(9676..10962)
                     /locus_tag="NBH55_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1509569.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            11235..11813
                     /locus_tag="NBH55_001581"
     CDS             11235..11813
                     /locus_tag="NBH55_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCD1509570.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11813..12742
                     /locus_tag="NBH55_001582"
     CDS             11813..12742
                     /locus_tag="NBH55_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1509571.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12739..14475
                     /locus_tag="NBH55_001583"
     CDS             12739..14475
                     /locus_tag="NBH55_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCD1509572.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14538..15374)
                     /gene="pheA"
                     /locus_tag="NBH55_001584"
     CDS             complement(14538..15374)
                     /gene="pheA"
                     /locus_tag="NBH55_001584"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCD1509573.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(15371..15880)
                     /locus_tag="NBH55_001585"
     CDS             complement(15371..15880)
                     /locus_tag="NBH55_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCD1509574.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15873..17168)
                     /gene="aroA"
                     /locus_tag="NBH55_001586"
     CDS             complement(15873..17168)
                     /gene="aroA"
                     /locus_tag="NBH55_001586"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCD1509575.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(17181..18272)
                     /locus_tag="NBH55_001587"
     CDS             complement(17181..18272)
                     /locus_tag="NBH55_001587"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCD1509576.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(18289..19455)
                     /gene="aroC"
                     /locus_tag="NBH55_001588"
     CDS             complement(18289..19455)
                     /gene="aroC"
                     /locus_tag="NBH55_001588"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCD1509577.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19457..20521)
                     /gene="aroB"
                     /locus_tag="NBH55_001589"
     CDS             complement(19457..20521)
                     /gene="aroB"
                     /locus_tag="NBH55_001589"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCD1509578.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20554..21576)
                     /gene="aroF"
                     /locus_tag="NBH55_001590"
     CDS             complement(20554..21576)
                     /gene="aroF"
                     /locus_tag="NBH55_001590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCD1509579.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         6366 a         4808 c         3832 g         6984 t
ORIGIN      
        1 gaaaaacaaa gtctttattt ctattattta cctgtaaccg caaagtagtt gccatcggga
       61 tcagcaaagt taaagacata ttcttctcca agctgtacca tatcacctaa tgtgactgat
      121 tgcgtctgca tttttttata tagtccaaag atatctttac tgtaaaacat caaagatggg
      181 gcactagtag ctgtgccagg tgaatgggac tcaacaaatg ctcgatcata taacagaaat
      241 gaggtttgac tgtcctcaac tcctacttca attataagcg tcccatccat ttcttgacga
      301 tctttttccg caaagccaac tgctctccaa aacgttgcat ttttttctac atcgtccaca
      361 taaagcatga tttttatttc attcgtaaac atctgtttcc tcctaaacga tctgttctaa
      421 cattctcaac tttgtcagtt taccatagga aaatgggaaa atctacgaaa gctctcaaca
      481 gaatcgtcct acttaaatac gcttttcttt aataataaat aagttggttg tttttgttat
      541 ttgcgctttc acttttcgga ataaataatg tgtatttata tggaataaat aaaaaaagag
      601 gtcgtgacta aaaacaagtc acaacctctg gtaaacggtg ttctcctgac aactcctcgg
      661 tcacaagaca caatattcaa caaacatgag aagctatttt ttgaaattgc tctttgtgac
      721 tcagagtgaa acggcaggct cacgacctct ttcttaaacg gtgttcaaaa gcttgcatct
      781 gcggtaataa gctcaaccaa acgtaaaata tgaggaacta tttttgtttg gctgttctta
      841 ttacttgatg cagggcagct ttttcacaac ctcttattca cggtgtttca gaagtagctt
      901 ctcggaaata agccgaaatt tcacaaaaat ttgagaaact atttttagga aacttcttct
      961 tatttcttaa agctgaccac ttctatcaca acctcttttt gactatgcat tcacatcgat
     1021 cactttagaa tcatcattga tgacatcgcg atctaaatct ttgatgcgtg cacttgtcac
     1081 gataccagct accatgctac cgctgacgtt tactgctgta cgtgccatat cgattaatgg
     1141 ttcaacagag ataactaacc caacgattgc tactggcaag ttcattgccc ctaaaacgat
     1201 cagtgaagca aatgttgcgc ccccaccgac tccagctaca ccaaaagaac tgattgttac
     1261 aattgccaca agcatcaaaa taaattccaa actaaagaca ttcactccag ctgctggtgc
     1321 gacgatcgtt gccaacatag ctggataaac gccagcacag ccgttttgtc caatagacaa
     1381 accaaaactt gctgagaaat tagctgttgc atcatctact cctaaagctt ttgtctgagt
     1441 ctcgatattc aatggcaaag ctccagcact tgaacgtgaa gtaaacgcaa agctcaatac
     1501 cggaaaggct tttttgaaat agttgattgg attgactttt gcaccaatca aaatcagtga
     1561 gtggacaatc aaaacagcaa gcaatgctac gtaagaagca acgacaaatt tacctaaatt
     1621 gacgatagct gcaaaatcac ttgttgccaa aacgtttgtc atcaaagcaa atactccata
     1681 aggtgtcaaa cgcaaaacaa gcgtcacgat ccgcataatg attttataca gactgtcgac
     1741 taaatctgaa aagaatgctg cttcttttgg cgcttttctt ttcacgccta gataagcgat
     1801 tccgacaaat gccgcgaaaa taacgactcc aatcgtactt gttgcacgac tacctgctaa
     1861 atcagcaaaa acgtttttag ggataaagga taaaatctgc tgtggaatcg ataagttttc
     1921 aactgtttcc tgacgtgttg ccagttcaga gattcgtgct gtttctgcag ctccttgtgt
     1981 gaatttagct ccttggagtc cgaacagcat tgttgaacca attccgatca atgctgcaat
     2041 agctgttgtt ccaagtaaag aagtcaatac agtcgcacta attttcccta gcttttcaga
     2101 aactttcatt tttgtgaatg caccaatgat cgaaataaac actaaaggca taactagcat
     2161 ttgcaagaat gatacatatc cttgacctac aatcccgatc cattccattg attgagtaac
     2221 gactttgtcg tctacaccaa agatcaattg aagcagaccg ccaaagacga taccaactcc
     2281 taatgctgta aagacgcgtg ttgaaaattt tgtatggcgc ttttgcatac gatagaacac
     2341 atataataat gccgcaaaaa ctagaaccac taaaactgtg atgagtgttg tcatttttct
     2401 cctcctcata ttattaatga caagtccatc gaaggaagta aaggttcccg agaatgattc
     2461 ttcaaccatc ctcctgaaaa aatttttttc agtgactttt atattttaga cgatttttat
     2521 cgaaatgaca ttctttttgc ttacaattag taataaaaaa acatacctgt gatagcttta
     2581 atctatcgca ggtatgtttt tactgtctat tcttgttttt ctgattgaaa attcaatttt
     2641 accccaggct gatctaaggc gatctctgtc acctcataag acattcggcg tacgatatcg
     2701 aataacggtg ctactagctc gtccacttct tcttgtgtga catcattttg accttcaatt
     2761 ttccgattga ggatatgatt gatctgactc acacttccag ataaaacata tgtatcaaac
     2821 actaaacgga actctaagcg tgctcctacg atgctatgtt ctttcggata gttttcgatc
     2881 gtttctttta aaggcgagaa gcctactttc aaatctgttt ctacttcttg gttcggcaat
     2941 ctcatatcat aatgaaatgc ttctacaact tctttttgtc ttctgatttc catataattt
     3001 tatcactcct ttatgaaaat tataacatat ctcctttgaa aggggatact actttttaat
     3061 gatgctctgc aaggtaagga tttcattcag atttcctacc acataatctg ccgggatctc
     3121 acccagaaaa tgatcgatgt cgtaaagaca agtagcgact cctgcatttt tccctgcttc
     3181 gatatctaaa cgacgatcgc cgatcatcat cgtgtctgtt ctttctaaat gaaaagtatc
     3241 gatcaaataa tttaaagatg ctggatcagg tttacgagga aagtcctgat cgatccctac
     3301 tacttcctca atcagatgtg ccaatcgatg ttcttttaac aggccccatg ttgattctgt
     3361 caaacggtgg gtcaggatga aatgacgacc gccgttgtct tttagcattt ccagtacttg
     3421 tttcgtttct ggaaatggac gggaaacttc cgtactttct ttttcatatc ggtgaaataa
     3481 ttcattaaac tctctggcat tcaagtttga ttcattgatc aaataactag tagaatactt
     3541 tttcattttg aagtagattt cttttttgtc catactgata ccaaaatctt ttagtgcttg
     3601 ccatgcacca tctaccattg cgggatacgt atcaaacaat gtaccatcaa agtcccatat
     3661 ataatttttc atgttccttt tgttccattc ctttgcttct tttttctatc tctcactttt
     3721 agataaaggt gttctttaaa gtattttttt gtccagaatt gtcaaatcat ctgctaatgt
     3781 atactggatt ggttgtgcat ttgggatatc gatcgtatcc atttgatctt caggaacatc
     3841 ttctaaatat tttgtcagtg ctcggatgct attaccatga cccacaacta gtaaatcttg
     3901 ccgatctttt agttttggtg ctagatgatc ttgccataag ggaatgatcc gattcactgt
     3961 catttccaga ctttctccgt atgggatcaa atgcggatcc aagtgatcat atctgcggtc
     4021 aaatctattt tctgcggtta acggcggaac ttccgtatat cctctgcgcc atgtttttac
     4081 ttgatctttt ccgtatattt ctttcatctt atctttgttt ttacccacaa gatccccata
     4141 atgccgttca tttagacgcc atgtttttat tataggaagc cataactgac ccatttcttc
     4201 tagtgcgact tgtccggtaa gaatcgcacg tttcaaaaca gaggtataaa cggcatcgaa
     4261 ttgaagtccg gcttcaagca ttttttgacc ggcaactgct gcttgttctt ttcctttttc
     4321 tgttaaggcg acatctagcc atcctgtcca gtaattttca aaatttgctt cgctttcgcc
     4381 atgacgtaat aatactaatt tcattctttt ctcccttaaa cattcgtttc gaattttata
     4441 ccacatgttt tgcatgtcac acgtattcgt ccttttcctt taggtgcacg ctgcttttga
     4501 tgacaattcg ggcactcaaa aaaagtataa ctttttcgat ccattttgac ttgctttttg
     4561 ttttttatct gatgatattt ttctttgact ctatcaagat atttcaaata acgttgattc
     4621 tcatttagtc tggggtagat tttttttgat aggaaacgaa atatcatcca tccgaaaaag
     4681 acgatgaaca acaattgtcc gattctatag ggcaaccaac gacttagcaa actaaagaca
     4741 agcgcagcga tcaatagacc gcgattcaac tgatcaaatt tcgcatatct tccctgcata
     4801 agtttgtgat agttctgctg aaatcgtatt agcctttcta accatgtttg catcatatta
     4861 tctcctccat catctaatat ttattatgac aaaaaaatcc aaaaagaaaa agccttaatt
     4921 ggccttttcc attgcaactt ggtgaaggac attccgattt ctggtaaagt caatcacaat
     4981 cactttcttt ttatcttctc tgttgactac tcgaaatgtg tccgtcaatg gaagaaacag
     5041 cttcttagat ggtagctgtt gcatacgtat aaaacgatgg atcatcgtaa tcaattgagg
     5101 aaattgaata gacgtgtgaa gcagacctgg atattttttc aacagctgtg tcgttttgat
     5161 cagattccgt tttttactga tcacttttaa aaagcttttt agaatctttt gctgacgtct
     5221 aaaaatagaa aaagaatttt cgtcatctat ttgataacgc atttgttttt gcagttctgc
     5281 tgcagtcaga tggatcttcc catcgtttgt atcgatcatt aaaccttctg gaaacagttc
     5341 aaccactgct cgatcccaag attcaagaga aagctcgata taatctttta ctggtaattc
     5401 caattctacc ttaaaaaagt gcaaaacagc cgacagccct tctgcttgat agatttctcc
     5461 taatgttagc ccctctagat ggaactctgg tgaaaaatgc agcaatgttt tattttcctg
     5521 caattgaaca tatagaaatg tagatcgctc aatcagagta tcttttaaaa caactaaaaa
     5581 attcattttt ctcccccttt tttcatcatt tttaaaatag catattttga aaagaaagtc
     5641 tgatgcttag agtagaattt cataaaaatt tactatttca taagtattca cgagaaaaat
     5701 acaaacaaaa aagcggaaat aacgctcatc cgccttcttg tttgtgttta tttcctcttc
     5761 aaacgcttgt ctatgattca gacttttctc tatcgttatt ttcatctaaa agttcttgca
     5821 ttccgataat ttctcgatat tgatgaatct tagtgatacc aatgactttg tctacatcaa
     5881 tactaaaaca aataccatta cctggaatat cgatgttgat gccttgacca atcgcatcca
     5941 ttactttatt cgtcaaacta tcgggtacta atgtcaacac aatatctttt tctggcgaaa
     6001 tgtccaaacc taaaaatttc cctgaatcat gtacaccaga acctcttcca tgtaagatcg
     6061 ttccgcctgc tgcacctgct tcttttgaaa agtcgataac atcttcgccc attcctccgt
     6121 caacgatcgt cacaatcatg tgaagattca atgctacaag atctttcatt tgctttcccc
     6181 caatttttgc aatctttctt tttcgattct gtctttttca tcttttcgtt tataaatata
     6241 tcctagtact aaagtggata atattggtgt caatgcaatc attgcaacca caccaaaccc
     6301 gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt gccacgctca ttgataaaat
     6361 aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta tcaaaagcca ttgctaaaaa
     6421 gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg tatcctggga tcaagaaata
     6481 ataaagagag aatccgacga gcaaacgcca catagacaat cctactgctg cgccgatccc
     6541 aattgaaatt accatcagca tgattttcga tcggattctc ccctcgctca attcttcaac
     6601 ctgtttgatc attacatgaa tcgccggctc agcaaaccct actaagaacc ccattaaaaa
     6661 gccaagggga accagtatat aattgtaagg caatcccgca atagcactgc ctaaatgctg
     6721 tcctaccggt acataagcca tattgactcc gtgcaggaaa aggatcaacc caatagtcgt
     6781 tatcaagaaa cctttcataa tgtcaaagaa acgctttttg cgcatacgaa aactaatgat
     6841 attcattaaa acaaatagga ttacgatagg tgccactgcc aaaatgactt ccatggtaac
     6901 gatttcgatt ccatctatca cttgctgcca gaaactcatt ggaagatcac ccccaaaatc
     6961 atcacggcaa gaatcggccc taacgaagca ataccgacca ttccaaaact gtcattttca
     7021 tttttctttt gtttgatcat actagtcatc cctccggcaa gggccaagat aaatggaaca
     7081 gttactggac cagtagtcac tccccccgaa tcaaaagcaa tcggcataaa ttcggaactg
     7141 gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag ccatcaattg ataataggat
     7201 agcttgaata cgacccgaag cagcgcaaag gctagaaata ctccagtgcc gacgctgacg
     7261 atcccaatca gcaacactct gccaatctct ccgtcagata tatcatatac ttgttgtccc
     7321 aaaacttgca cggaaggttc agcaatcgtg atcacgatcc cgatagcaaa tcccaaactg
     7381 accaaaacgg tcaaactttt ctttttgatc atatatttcc ctactagatc tccgacttcc
     7441 atcatagaat aatctgctcc aaacaaaaag agcgtcattc cgatcatcat gattgccgca
     7501 ccaaccagaa atgaagcgag gtcttctcct tctaacggtg caaaaacaaa cgttaaaatg
     7561 acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt ttttagcatg
     7621 tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg agcacaaact
     7681 taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa aagtatcttt
     7741 ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt ttacagagcc
     7801 gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt
     7861 agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag cacccatagc
     7921 agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta agggccataa
     7981 ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg tatttttagc
     8041 cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta atcgagcggc
     8101 ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga tcaaaaaagc
     8161 agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt
     8221 caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa ccatcatcga
     8281 tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct caaatccata
     8341 tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga tcatcgctga
     8401 attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa aattgatgat
     8461 caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat tcgtcattcc
     8521 caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta atgctgtata
     8581 ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga cgttggggcg
     8641 ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac tttcgtttgg
     8701 attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc cgcatatcca
     8761 aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt ggtcgttgca
     8821 tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa tgtcccgatc
     8881 gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt cactttgata
     8941 aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc cgaatcaata
     9001 ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca agtgatcgaa
     9061 agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc
     9121 ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag acttttcatg
     9181 atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt tcgaaacaac
     9241 cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc tagaaacaac
     9301 atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt tttaaaagtt
     9361 gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt gacaggggga
     9421 ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat gattgatatc
     9481 aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt
     9541 cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag acaatatctg
     9601 taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca
     9661 cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg tatctgctag
     9721 taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta cgactgcttc
     9781 aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa
     9841 atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag cttcttgttc
     9901 attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc ccatcagttc
     9961 ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc cagctactcc
    10021 tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt gcagattttc
    10081 tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg ctccttggat
    10141 cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt gtatcgcagt
    10201 tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa gagtggcaaa
    10261 caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct ccccatcttc
    10321 agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa ggtcagaagg
    10381 attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta cttgaatata
    10441 ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg ccatcagatc
    10501 cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata ttttatctcc
    10561 tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa tagcagacaa
    10621 attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat cttcaatcaa
    10681 gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca gtttctgaac
    10741 gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct cataaacttt
    10801 ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga cagtgacttt
    10861 ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac ctgatgctgc
    10921 cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc gctccttttc
    10981 catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa acggtttctt
    11041 tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa atcaactatg
    11101 acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt tctttaaaat
    11161 aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag actattctta
    11221 aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag acgattttag
    11281 atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata gggacttttt
    11341 ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc gtggtcacag
    11401 atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa cagcaacaac
    11461 ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt aaagattctt
    11521 ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat ttttttctaa
    11581 attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc ccaatcacca
    11641 atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa actcgtccat
    11701 tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg aaaaacggcc
    11761 attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat tgatggatag
    11821 cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg gctatgtaat
    11881 caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc aagaagtttt
    11941 tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca atgaaatatt
    12001 ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa agaaccttgc
    12061 ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa gtagttattt
    12121 aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga atttcttcac
    12181 gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa tcaatcgttt
    12241 ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac tttctttgct
    12301 caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt tttctattct
    12361 tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa aagacaattt
    12421 cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag aaatcaattt
    12481 acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca tctttactga
    12541 aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta aagccaagct
    12601 ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg gctataccga
    12661 actgggttat ttctccagag tttttaaaga agagacagga atgacacctt ctcaataccg
    12721 taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt tgccaaactt
    12781 ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt gtttatttat
    12841 catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt ggtactaaaa
    12901 aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt agccgctttt
    12961 gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca tatggaacag
    13021 cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat ccatccaatg
    13081 atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg aaacgacatg
    13141 ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag agtgaatgaa
    13201 cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg cgataagaaa
    13261 gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt ttacggatat
    13321 gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt agtaaatcct
    13381 gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc aaatgaagaa
    13441 aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga aacaacgatt
    13501 tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac attactcagt
    13561 caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat tattttgtat
    13621 aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga tcgacacaca
    13681 aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg cttaaaagct
    13741 actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat caatcaattg
    13801 ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa gaaacgttcg
    13861 cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa tacgctgact
    13921 agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg aatcgacagt
    13981 ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc agtcaaggaa
    14041 gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg cgaaagaatc
    14101 tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa tatgatcctt
    14161 caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca aggattcatc
    14221 acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga taacggcgat
    14281 ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat cggcatcagc
    14341 catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac gatccaatca
    14401 gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa aaatcaatca
    14461 gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt aaataaatca
    14521 atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg ataacagccc
    14581 aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc tactaatgtc
    14641 attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct agttttcaat
    14701 ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc cagcatttta
    14761 tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt tactggagct
    14821 gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg gttcaactca
    14881 ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt ttctaatgaa
    14941 gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc agcagttgtt
    15001 gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg ttgtgattgt
    15061 gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt gcctaaaagc
    15121 tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg tttggaaaga
    15181 agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac gacagccaga
    15241 tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata cgcagtcaat
    15301 tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc tggccctaga
    15361 tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc ttttctggtg
    15421 tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa agtcgttgtg
    15481 ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga atagacgttt
    15541 catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa agaacaatcg
    15601 gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca ccagtcgcga
    15661 taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt cgaaagcttt
    15721 tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc tcaatctttg
    15781 tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg attgttgttt
    15841 ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc tcctgacaca
    15901 agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc agctccttct
    15961 agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc gattcgatga
    16021 tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat gatcagaccg
    16081 tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac cgcatcaatt
    16141 ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat tccttctgct
    16201 tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat gatttctcca
    16261 ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga ttggttctgt
    16321 tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc taagatacca
    16381 gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg aacaagaagt
    16441 cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat ctgttgacca
    16501 gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat ccttccgcca
    16561 aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac gattacggat
    16621 gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc actggctaca
    16681 ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat tggtagatac
    16741 tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc catcactcgc
    16801 cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa aggctgtcca
    16861 gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc aattgattcg
    16921 gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc ttcctcttct
    16981 tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc ttctgcgcgc
    17041 aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat gctgcgatgg
    17101 gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat ttgttgtaat
    17161 aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt agacataatg
    17221 attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag tattcctaaa
    17281 atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc ttttccgatc
    17341 agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa taaatcaaaa
    17401 aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc ttttgcccga
    17461 ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc tatttgtgtc
    17521 atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct caacaaaata
    17581 tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa gcctcctgct
    17641 gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt gactaagcca
    17701 gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg ttctcttgct
    17761 gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt ttcaacatct
    17821 tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag tttagcatct
    17881 gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac aaaccgaaga
    17941 tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc cgtaatgatg
    18001 acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt tgtacggacg
    18061 ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc acttgcaacg
    18121 taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact atcccaacca
    18181 actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc accaatcagt
    18241 ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt agtaatgttt
    18301 caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat cacttgggaa
    18361 ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa cgacgctagc
    18421 tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt cttttgtatc
    18481 gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga ccccgcgaat
    18541 gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat tggtacgtcg
    18601 gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc ctggtaatga
    18661 accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac taaccacagc
    18721 ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta atccagctgg
    18781 aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt tctttgtctg
    18841 atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta cttctgacgc
    18901 ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg cttggattcc
    18961 accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa ttttcttggc
    19021 aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt caagcacatt
    19081 ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc ctggacgcgg
    19141 acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag acatgacagt
    19201 tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac caattgtttt
    19261 gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca tccttcctcc
    19321 tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt ctgacagagg
    19381 taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg attctcccgc
    19441 agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct gtgggaacag
    19501 gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg cttcctcttg
    19561 ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga tcgtacgtaa
    19621 tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca gttggagaca
    19681 agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact ccttctccat
    19741 gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta tgaccaaaat
    19801 ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt cgtttgattt
    19861 ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg tctttcagct
    19921 cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg atttcagcga
    19981 ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca atcaatacac
    20041 cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg actgctgtct
    20101 tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga acgaaatgga
    20161 tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt cctccaccaa
    20221 gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg tataactcag
    20281 ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg actggttcaa
    20341 aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca acgtttgtat
    20401 cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat ccagcctgtt
    20461 ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt acagtaagca
    20521 ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa tcttttccat
    20581 tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt cattcaacga
    20641 ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga tcatgccatc
    20701 tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa ttcctgtacc
    20761 atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg ggacagcact
    20821 taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt cgataaaaat
    20881 gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt cattgatcgt
    20941 accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag cagatagcag
    21001 tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact cagccaccat
    21061 atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt catcagccgc
    21121 ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg gagaagtacg
    21181 tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca ttcgcgcaca
    21241 ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca cgaaactgcc
    21301 gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga attctcgaga
    21361 agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat agcgtgtaaa
    21421 tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc gttctgttcc
    21481 ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt tttttatttg
    21541 ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt tcttttgttt
    21601 acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat taattcctac
    21661 taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta tgacccagat
    21721 aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat cttcaacgag
    21781 ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct ctacgtggct
    21841 gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg tttgtaccca
    21901 cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc agttttgtaa
    21961 gttttctaga tttcataaaa aatcgctcca
//
LOCUS       DAHHPA010000047        21047 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.048, whole genome shotgun sequence.
ACCESSION   DAHHPA010000047 DAHHPA010000000
VERSION     DAHHPA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.048"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..640
                     /locus_tag="NBH55_001591"
     CDS             <1..640
                     /locus_tag="NBH55_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCD1509580.1"
                     /translation="QDSCALVHRDPKEVTLVAVTKSVESAQAEELVRAGVMNCAENRV
                     DKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSLRLAEEIQKRLEKE
                     LSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMAPKEASDEQIHKVF
                     GTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVGTALFKDE"
     gene            654..1253
                     /locus_tag="NBH55_001592"
     CDS             654..1253
                     /locus_tag="NBH55_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCD1509581.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            1268..1555
                     /locus_tag="NBH55_001593"
     CDS             1268..1555
                     /locus_tag="NBH55_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCD1509582.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            1604..2386
                     /locus_tag="NBH55_001594"
     CDS             1604..2386
                     /locus_tag="NBH55_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCD1509583.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            2407..3114
                     /locus_tag="NBH55_001595"
     CDS             2407..3114
                     /locus_tag="NBH55_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCD1509584.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            3376..6174
                     /gene="ileS"
                     /locus_tag="NBH55_001596"
     CDS             3376..6174
                     /gene="ileS"
                     /locus_tag="NBH55_001596"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCD1509585.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(6223..7749)
                     /locus_tag="NBH55_001597"
     CDS             complement(6223..7749)
                     /locus_tag="NBH55_001597"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCD1509586.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(7764..8411)
                     /locus_tag="NBH55_001598"
     CDS             complement(7764..8411)
                     /locus_tag="NBH55_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCD1509587.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(8595..8924)
                     /locus_tag="NBH55_001599"
     CDS             complement(8595..8924)
                     /locus_tag="NBH55_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCD1509588.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            9101..9829
                     /gene="sfsA"
                     /locus_tag="NBH55_001600"
     CDS             9101..9829
                     /gene="sfsA"
                     /locus_tag="NBH55_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCD1509589.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            9845..10858
                     /locus_tag="NBH55_001601"
     CDS             9845..10858
                     /locus_tag="NBH55_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1509590.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            10858..12135
                     /locus_tag="NBH55_001602"
     CDS             10858..12135
                     /locus_tag="NBH55_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1509591.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            12198..14900
                     /locus_tag="NBH55_001603"
     CDS             12198..14900
                     /locus_tag="NBH55_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCD1509592.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            15052..15369
                     /locus_tag="NBH55_001604"
     CDS             15052..15369
                     /locus_tag="NBH55_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1509593.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            15399..15719
                     /locus_tag="NBH55_001605"
     CDS             15399..15719
                     /locus_tag="NBH55_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD1509594.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            15827..17287
                     /locus_tag="NBH55_001606"
     CDS             15827..17287
                     /locus_tag="NBH55_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1509595.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            17355..17576
                     /locus_tag="NBH55_001607"
     CDS             17355..17576
                     /locus_tag="NBH55_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509596.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            17607..17789
                     /locus_tag="NBH55_001608"
     CDS             17607..17789
                     /locus_tag="NBH55_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCD1509597.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            17789..18202
                     /locus_tag="NBH55_001609"
     CDS             17789..18202
                     /locus_tag="NBH55_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCD1509598.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            18325..19506
                     /locus_tag="NBH55_001610"
     CDS             18325..19506
                     /locus_tag="NBH55_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1509599.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(19577..19741)
                     /locus_tag="NBH55_001611"
     CDS             complement(19577..19741)
                     /locus_tag="NBH55_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509600.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            19895..19968
                     /locus_tag="NBH55_001612"
     tRNA            19895..19968
                     /locus_tag="NBH55_001612"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19929..19931,aa:Arg,seq:cct)
     gene            complement(20037..>21047)
                     /locus_tag="NBH55_001613"
     CDS             complement(20037..>21047)
                     /locus_tag="NBH55_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1509601.1"
                     /translation="ELEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYE
                     DTLMNIKKYMPAVLISDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGL
                     IPFDPTRKAVIKGKDSLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATG
                     MRFAELLGLTWEDIDFEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKK
                     FKINQKELFENFEIKNPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLR
                     HTHASILLYQGVNILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKN
                     DN"
BASE COUNT         6843 a         3629 c         4352 g         6223 t
ORIGIN      
        1 tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac
       61 aaaatctgtt gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc
      121 agaaaaccga gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat
      181 aaagtggcat ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat
      241 tgattacttt catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga
      301 gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat
      361 ttcaccagat gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt
      421 tgttgggttg atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt
      481 tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg
      541 tagtgaatta agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac
      601 atttatccgt gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc
      661 ttttaaacag aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat
      721 accaagatta tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac
      781 agaatcaatc aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga
      841 cttcacgtcc tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac
      901 tacatcaatc acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac
      961 aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag
     1021 ctatggcgat tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag
     1081 tagaagaaaa acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag
     1141 atggagatat caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga
     1201 ttgacagttc aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg
     1261 aactacgatt tatactattg cactattact tcatttactt caacgggcag cctatttcta
     1321 tagcattatc ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg
     1381 gctaggacga ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc
     1441 tttgaggatt ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc
     1501 cgtacaaggt ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa
     1561 gcaacaaaat ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata
     1621 ccagcacttt cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca
     1681 ggttcaagga caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat
     1741 tttagaaaca ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga
     1801 acaggcagaa agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga
     1861 ttttgaaaca gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg
     1921 gaaaatcctt ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat
     1981 ctcggacggt gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc
     2041 acaagtaaca aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat
     2101 gattatgccg aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga
     2161 cagtgtgatc tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc
     2221 tggtaaagta aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt
     2281 agatattatt tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac
     2341 gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt
     2401 ttactcatgg ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg
     2461 ggatataatc aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa
     2521 ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag
     2581 tacttcaatg aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct
     2641 gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac
     2701 aaagcagatg agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca
     2761 gaagaaaaag caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca
     2821 gaaactgaag atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt
     2881 gagtctcaat tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc
     2941 agctacgtga ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat
     3001 gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt
     3061 gatacacagg ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga
     3121 aaagcacttt gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca
     3181 tggtgaaaga tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct
     3241 gacctcgtta ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta
     3301 ccacggcatg ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa
     3361 ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattccct
     3421 atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga
     3481 atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga
     3541 cctccatatg ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac
     3601 attatcattc gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat
     3661 acacatggat tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg
     3721 tccatggcag aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa
     3781 cgagcagatt tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta
     3841 gatccttctt atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaggctat
     3901 atttacaaag gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa
     3961 gctgaaattg aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa
     4021 gacggcaaag atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg
     4081 acattgccag caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa
     4141 gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc
     4201 ggttgggaaa atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact
     4261 gcaagtcatc cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta
     4321 gacgctggta ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct
     4381 ggtagcaaat ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa
     4441 gaagcacctg gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta
     4501 ttaaaagaaa aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat
     4561 tggcggacga aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat
     4621 aaattccgtc agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt
     4681 aaaacacgtt tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt
     4741 gcatggggag taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca
     4801 gaaacaatcg aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa
     4861 aaagaagcga aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa
     4921 tttacaaaag aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct
     4981 gttttacgtc agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac
     5041 caatatcgtg gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca
     5101 ccatacaaat cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt
     5161 aaatctttag gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc
     5221 cttcgcttat gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc
     5281 ttaagtcaag tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg
     5341 aatacagaag atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta
     5401 gacaaatata tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac
     5461 gacaaatata acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta
     5521 tcgtcattct acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat
     5581 caacgtcgtt gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg
     5641 actcctatca ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag
     5701 tacgttcagt taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg
     5761 gatacctgga ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca
     5821 agaaacagca aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa
     5881 caagttcgtg aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca
     5941 ccagactact ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt
     6001 gatgatgttg ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa
     6061 gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa
     6121 atcgttgaag acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga
     6181 aaagggggtg tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc
     6241 acgttcacga taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag
     6301 ttcgaaagct gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt
     6361 ttggtcccaa gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca
     6421 atgagagaag ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc
     6481 tgggctattt tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc
     6541 aaatccttgc cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat
     6601 atagatcgtt agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt
     6661 tttgaataca ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt
     6721 caaacgtttg cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt
     6781 gaatttgcct gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg
     6841 atagcccttg aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt
     6901 ttctaacact tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt
     6961 acggatttct ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg
     7021 taaaatatga ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg
     7081 atcttctaca ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt
     7141 ccattgagcc tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc
     7201 ttttcctaaa taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg
     7261 ctggttcaat tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg
     7321 attgaagcca ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa
     7381 aaattgaggt gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc
     7441 taatcggtca gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga
     7501 ttgatagtaa aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg
     7561 gattgtttct ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc
     7621 gattacagca aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta
     7681 caatttacgc tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc
     7741 attagacatg tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta
     7801 aagctgacgg ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg
     7861 atgtcaacga tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac
     7921 aattcttttt catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc
     7981 aatgtttgtc ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga
     8041 ggacaatgtt caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc
     8101 acatgttcca atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca
     8161 actaaaaaaa cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg
     8221 cttgcagtca attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt
     8281 ttagaaatca tttcactgac agatgcagct gagacatcta gactagatac gatttgttta
     8341 ttgttgactt tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga
     8401 tttggtgtca tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa
     8461 aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac
     8521 aacaaaaaac gaaagatgat agagaagact cattctttcg tttataaagg atatatatat
     8581 acattgggag ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca
     8641 taaagagttt gttcgggata agaaaagata agataaaaac catactgatc acgtacgact
     8701 aaacgatatt catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac
     8761 gtgtaatcag atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta
     8821 ccttgatact ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata
     8881 aaacttgaga gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa
     8941 tcactccttt atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac
     9001 cgaataaaag ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa
     9061 attatgatag gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga
     9121 aatagctttt tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg
     9181 agaaataatt attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg
     9241 cgctgaagtg gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat
     9301 tgctgtaaaa aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc
     9361 aattgatggt attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt
     9421 atacaagcgt gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt
     9481 cggacaaaaa gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc
     9541 ttttcctgat gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc
     9601 acaagatggt tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc
     9661 aacgatccat gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag
     9721 cggagttcaa atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca
     9781 ggccattcca tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa
     9841 aataatgatc aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc
     9901 agctttaagc acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc
     9961 acaggaattt ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt
    10021 tttcaatatt actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag
    10081 tcaagctaag caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc
    10141 gacgccaaaa gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt
    10201 tgaagcagca agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt
    10261 aaaagacgag ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca
    10321 agtattagat ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat
    10381 ggatttaggt gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt
    10441 ccattatttc ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg
    10501 ttatgagaat tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc
    10561 agagatttat tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc
    10621 acaatttcat gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa
    10681 tccaatggca gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc
    10741 ttggggattg ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta
    10801 tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg
    10861 aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt
    10921 atctcacctt ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata
    10981 gtagcggttt ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta
    11041 aacgtggaac caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct
    11101 attcaagtag cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct
    11161 ttggtgggcg gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc
    11221 ttgattggta caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat
    11281 caagtgaaaa caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa
    11341 atcattgatc aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg
    11401 acagcagatc gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt
    11461 gacgtgacag cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg
    11521 atacgaaaga aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg
    11581 attttcttta atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt
    11641 gtaccggtag gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa
    11701 caattcaaaa agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta
    11761 gatattacag gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat
    11821 ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta
    11881 caagataata atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa
    11941 gagatttttt tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct
    12001 cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat
    12061 aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga
    12121 gcaaggcgca aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt
    12181 gccagaggag gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa
    12241 accaacaagg attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg
    12301 caagccgata tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac
    12361 cagtcgttta ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt
    12421 ctacgcaagt gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac
    12481 agcaggcaaa agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag
    12541 aaactggtac agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag
    12601 agatgctttc tgcagatgcg ccatttgttt ctttcaattg tgcggattat gctcaaaatc
    12661 cgcaactgct ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc
    12721 aagatagtcc cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc
    12781 accgtttacc gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc
    12841 gtccgcttgg agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga
    12901 ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct
    12961 tgccgaatct tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca
    13021 aacaagaagc gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct
    13081 tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct
    13141 gtgcaaaatc ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag
    13201 aagaactttc ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc
    13261 tcgaccgatt catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg
    13321 ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag
    13381 tcacttctct ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag
    13441 atgtggatgc ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag
    13501 atcttttgcc agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac
    13561 ggttagaccg tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta
    13621 cattggatcg tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga
    13681 aaaattttaa gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag
    13741 aacaagttga gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc
    13801 taggagaaag tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca
    13861 gacagactgc ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga
    13921 aagcttttaa tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat
    13981 atgtcaaaaa ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat
    14041 cattaaacac cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca
    14101 cgatgacaag taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct
    14161 tagaagatat ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca
    14221 aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg
    14281 gtgaaggagt agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg
    14341 tagaactgat ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct
    14401 tattggaaga atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata
    14461 tcccattttt ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta
    14521 tagctgatga tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg
    14581 atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga
    14641 ccgatttgtc gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct
    14701 tcatgttaga cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt
    14761 tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac
    14821 gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg
    14881 aaaataaaat aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg
    14941 cgcactataa aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg
    15001 catgcttctt gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa
    15061 aaaacaatca tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg
    15121 caaaaagcag cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat
    15181 gcagataata atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt
    15241 ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc
    15301 aatatgcaag actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta
    15361 atgaaataaa tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaatctag
    15421 aagccatcat ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga
    15481 ttgcggctgc taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag
    15541 aatcacttat acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa
    15601 atcatatgca agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg
    15661 cgtttactga tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa
    15721 tacaacttct tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat
    15781 aaggagaact ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc
    15841 aagttggatg gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt
    15901 agttgctttg cgtgatgctt ttatcggcac actgcccgca acaatggcgg gatcagtcgc
    15961 tgtaatgatc aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga
    16021 cagtttagca gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg
    16081 ttttgtctgg aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta
    16141 taatcttgcc cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac
    16201 cttgattcaa ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc
    16261 ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac
    16321 actgtcagtt ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt
    16381 tacagttatg atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa
    16441 cattacgatc aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat
    16501 tttaccagcg acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt
    16561 agctgatggg caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg
    16621 attatctcaa gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt
    16681 ctttggtatt catggaccaa atgtattggc accagtgcta gagggaatct ggggacaagc
    16741 acaactaatc aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc
    16801 cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc
    16861 ttttgcatgg tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt
    16921 ctctaaacgt gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa
    16981 tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc
    17041 attcttgatt gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct
    17101 agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt
    17161 agcaacagga gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt
    17221 cttgatttgg gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca
    17281 acaataagat gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc
    17341 gaggaggaac tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat
    17401 tgttagaagg atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca
    17461 agctgttctt tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac
    17521 taatcaaagc acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa
    17581 tggtttaaaa agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg
    17641 gttctttgat cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta
    17701 tggtcacagg tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa
    17761 gagaagaagc aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc
    17821 atcgtttttt tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg
    17881 caaatcagta actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa
    17941 taaccgggct cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac
    18001 atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac
    18061 ctgtgaggtt cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga
    18121 tatgctttta ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga
    18181 tatgatcgaa gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg
    18241 aagtaagcta gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc
    18301 ttttttgtag gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg
    18361 attgaggaaa tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg
    18421 cgagaacgtt ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag
    18481 gatcggctaa caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag
    18541 tacctaatgg ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg
    18601 atcaatcaga tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct
    18661 tatctggcgc cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat
    18721 aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa
    18781 ataaagcgta tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg
    18841 ccggaaaacc aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag
    18901 caaaaaggca attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat
    18961 atcgtttatc agcttgcaac gtcttccttt attattgcgg agtggggatt tttggatcat
    19021 gatgcttttt gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt
    19081 gaatattcat gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct
    19141 tctgtaatga tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa
    19201 aattatccat ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat
    19261 gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca
    19321 aaagtagaac aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta
    19381 ttattagggt atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa
    19441 gaatatgaag aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat
    19501 ttttgataaa aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg
    19561 catttgaagg aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt
    19621 cttttgtttt agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc
    19681 gatttttttt agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca
    19741 ttggaaagga cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact
    19801 agtggaaacg ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa
    19861 aaaacaaaca agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc
    19921 aatcgcctcc taagcgatag gtcgctggtt caattccagt caggaacgta taaataaccc
    19981 ctgtatcctt tgcggtacag gggtttgttt tgtatttgtg ccaaaaatgt gccaaactag
    20041 ttatcatttt tatataaact atcgaacaca gtattgattt tttctttatc ctgatcttca
    20101 agctctcgaa caatatgaag ataagtagac attgtagttt ctaaactgct atgtcctaaa
    20161 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgtgtatgc
    20221 cttagtccat gcaaagtaaa ttgcttttca atccctaatt cttttaattt tttgcgcaaa
    20281 tatttgctga cggcgtttga tgaaactaat ccatttttta agttgaaaaa aacaaaatta
    20341 tgagggtttt tgatttcaaa attctcgaat aattcttttt gattgatttt aaactttttt
    20401 aagagatcaa tcgtatgttt atcaatggaa attttcctat ttgaagtttc gttttttgtt
    20461 tttccccatt catttaattt ataattccat gttttattaa tagtgatgat ttgatcttcg
    20521 aaatcgatat cttcccatgt taatcctaga agttcagcaa atcgcattcc agtggcacca
    20581 gcaactaaca cgagcatagg agaagagtac tgcgcagaaa ggttttcttc tacgagtttc
    20641 attaaagatt taaattgatc ataatctaaa tatttatctt cttttggctt caatgaatct
    20701 tttcctttga ttactgcttt tctagttgga tcaaacggaa ttaatccttc ttctacggca
    20761 tctttcaacg acgctctaat atggttatta aacttaataa cggtagattt tacatggtct
    20821 ttagcatact tatttaaaaa gcgttgatat cccactctat ctaaatcaga aatcaatacc
    20881 gctggcatat atttctttat gttcattaac gtatcttcgt attttctata agtgatagga
    20941 gatactgttc cttctttata aagttgcatc caatcctcaa agtaatctga taataataga
    21001 tttttgcgat cccctttaag gcctttgttc aagttatatt ctaattc
//
LOCUS       DAHHPA010000048        20895 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.049, whole genome shotgun sequence.
ACCESSION   DAHHPA010000048 DAHHPA010000000
VERSION     DAHHPA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.049"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..635
                     /locus_tag="NBH55_001614"
     CDS             <1..635
                     /locus_tag="NBH55_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCD1509602.1"
                     /translation="QTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQEAEEI
                     MNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGISIHRR
                     DCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTVNALTK
                     NLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            646..1092
                     /locus_tag="NBH55_001615"
     CDS             646..1092
                     /locus_tag="NBH55_001615"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCD1509603.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            1263..2522
                     /locus_tag="NBH55_001616"
     CDS             1263..2522
                     /locus_tag="NBH55_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCD1509604.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(2611..3558)
                     /locus_tag="NBH55_001617"
     CDS             complement(2611..3558)
                     /locus_tag="NBH55_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1509605.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            3952..5244
                     /locus_tag="NBH55_001618"
     CDS             3952..5244
                     /locus_tag="NBH55_001618"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCD1509606.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            5259..7028
                     /gene="aspS"
                     /locus_tag="NBH55_001619"
     CDS             5259..7028
                     /gene="aspS"
                     /locus_tag="NBH55_001619"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCD1509607.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            7137..8012
                     /locus_tag="NBH55_001620"
     CDS             7137..8012
                     /locus_tag="NBH55_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD1509608.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            8278..10161
                     /locus_tag="NBH55_001621"
     CDS             8278..10161
                     /locus_tag="NBH55_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1509609.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(10236..10520)
                     /locus_tag="NBH55_001622"
     CDS             complement(10236..10520)
                     /locus_tag="NBH55_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCD1509610.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(10510..11244)
                     /locus_tag="NBH55_001623"
     CDS             complement(10510..11244)
                     /locus_tag="NBH55_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCD1509611.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            11373..11996
                     /locus_tag="NBH55_001624"
     CDS             11373..11996
                     /locus_tag="NBH55_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCD1509612.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            12188..13309
                     /locus_tag="NBH55_001625"
     CDS             12188..13309
                     /locus_tag="NBH55_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCD1509613.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            13316..16444
                     /locus_tag="NBH55_001626"
     CDS             13316..16444
                     /locus_tag="NBH55_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCD1509614.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            16463..19657
                     /locus_tag="NBH55_001627"
     CDS             16463..19657
                     /locus_tag="NBH55_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCD1509615.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(19803..20510)
                     /locus_tag="NBH55_001628"
     CDS             complement(19803..20510)
                     /locus_tag="NBH55_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1509616.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         6999 a         3681 c         4425 g         5790 t
ORIGIN      
        1 atcaaacaga agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga
       61 tggccaatcg tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag
      121 aagcagaaga aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg
      181 aaggtggcgt tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta
      241 atccgattcc tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc
      301 atcgtcgaga ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg
      361 aagtcgaatg ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt
      421 acggctataa tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga
      481 aaaatctcaa cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga
      541 cggttggtat ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac
      601 ctgatgtcta cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt
      661 caacgggtaa gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac
      721 ggcctacttg ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg
      781 gtaaaaaaaa ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt
      841 gtagaagatg tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg
      901 aaaaaaggaa atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta
      961 tacgaatcat tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc
     1021 ggagcagata tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct
     1081 aaaaacaaat aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt
     1141 tagactgata aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca
     1201 gcagtcggaa aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga
     1261 agatgaaaga agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa
     1321 aaggatacaa catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa
     1381 agattttcaa taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg
     1441 atccaaggaa tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg
     1501 gtcaaggtgg agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta
     1561 tggatctaat gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga
     1621 cacaagcact tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt
     1681 tctttacatc tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg
     1741 tcgaaaaatt agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg
     1801 atcacataaa aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg
     1861 aagaagctcg acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca
     1921 atgccacaga tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga
     1981 acatcatccg tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt
     2041 tcagtagacc agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt
     2101 ttattcaaga aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga
     2161 aagaagtaga agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat
     2221 atggaacaga ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt
     2281 atgtagggaa agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg
     2341 ggggaaattt tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac
     2401 gtgtgttttc cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga
     2461 cgattccatt caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt
     2521 agattagcat aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc
     2581 atattactat aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt
     2641 ttgataatct tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc
     2701 tggacgctta gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa
     2761 aacaagttgc gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc
     2821 ggttaaaatt tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc
     2881 ttcaccaaat tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag
     2941 aggaaaatag ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc
     3001 agcctgccga tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt
     3061 cgcttctttc aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc
     3121 ttcttttaat tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa
     3181 gtggatataa aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga
     3241 ttgtttgaga aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc
     3301 ttttgtagcg atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat
     3361 cagttcttcg gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct
     3421 attcaaagct gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt
     3481 atgtcctcca acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc
     3541 gattcttatt tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa
     3601 aaagctgaac taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac
     3661 tggtttttag taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga
     3721 aactttacaa gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga
     3781 cactctggag atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa
     3841 agagaaatct ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga
     3901 tgaacaaggg tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc
     3961 caaagaccaa aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc
     4021 gaagaaacag cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt
     4081 tttgaacact atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa
     4141 gagatgtatg atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca
     4201 gcaccaatcg ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg
     4261 ttcaaaactt tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta
     4321 cgtcaattcc atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg
     4381 gagagcatgg tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta
     4441 gtcatcaatt cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac
     4501 tatcttttac cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat
     4561 ccattacgcg tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca
     4621 tctatcttag attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg
     4681 ctggatgcat tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat
     4741 tacacacata cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg
     4801 atttgcgcag gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct
     4861 ggcttcggat ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta
     4921 gttatccctg catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat
     4981 atcgaagcat tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac
     5041 ttcatgaacc gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta
     5101 gtattggtga tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca
     5161 agccgaaaag aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat
     5221 gatgaaatga ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt
     5281 gcggattagt ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa
     5341 aaagacgaga ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc
     5401 aaatcgtctt taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca
     5461 gtgaatacgt gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc
     5521 ctaaaatggc cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag
     5581 caaaaacccc gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga
     5641 aatatcgtta tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg
     5701 cgacaacaaa agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac
     5761 cttacctagg gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc
     5821 atgctggaca tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga
     5881 atgctggatt cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg
     5941 gagaccgtca gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag
     6001 aaatccaaac ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg
     6061 aagtcacatt gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg
     6121 acaaaccgga tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag
     6181 tagaattcaa agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg
     6241 ctaaaggcgc tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag
     6301 gacaattcgg agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc
     6361 caattgctaa attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac
     6421 caggtgactt gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg
     6481 ctatccggac acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct
     6541 tatgggtaac tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg
     6601 ctcaccatcc attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg
     6661 caaaagtcta tgcagaagct tatgatatcg tcttgaacgg ttatgaacta ggcggaggtt
     6721 ctatccgtat tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa
     6781 aagaacaaat ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc
     6841 cacatggcgg tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata
     6901 atattcgtga agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg
     6961 caccaagtat ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag
     7021 aagaatagcc gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg
     7081 atttaattga aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga
     7141 aaaaaacatt cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg
     7201 tttatgcgtt gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt
     7261 caagtggtat tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat
     7321 tcgaagtacc ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt
     7381 ggttaaaaat cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc
     7441 tttgtatcca tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt
     7501 ttgggggggc tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg
     7561 gcggtctaga tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta
     7621 tttcgatcta cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat
     7681 atatgtttta cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata
     7741 ctaaacaaaa gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg
     7801 aaatccaaaa ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata
     7861 agcatgataa gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta
     7921 gagaagcaat gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga
     7981 tacttggtcg tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata
     8041 ctgctgtaga cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa
     8101 gaaaaactgc ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt
     8161 taaatttatg ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt
     8221 tcacttaata cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg
     8281 gaaaaacaaa aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca
     8341 ttagctgtgg tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat
     8401 ttgggcgttc cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag
     8461 gaatgggaac aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga
     8521 ctctatccgg cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag
     8581 cgagggctgt taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac
     8641 tggtggcaaa tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt
     8701 acgcatatct ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt
     8761 gtattgttga tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca
     8821 tccttcctct ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt
     8881 gtttattacg gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt
     8941 gtttggccaa gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat
     9001 atcgctggag gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat
     9061 tacaatttca tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt
     9121 gcagtgacag cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact
     9181 tggattggca aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat
     9241 gaagcaaagg tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca
     9301 ttgattctaa tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt
     9361 gattatggtc gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag
     9421 agcaaaccat gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt
     9481 acggcaccga aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa
     9541 agtaaaaaat tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac
     9601 caagcgatcc ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta
     9661 gaactaacgg catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt
     9721 tatccaaaag tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac
     9781 attgaaaaat taaaggaaca agatttgttg aaagacactg ttctgatcgg actaggaacg
     9841 aatggctcat tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa
     9901 gtatactggg tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg
     9961 cttgaacaga tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt
    10021 aatgaccata gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca
    10081 cattacattc acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt
    10141 gagacaaacg tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc
    10201 ctgaagtttt tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg
    10261 gttgagtgtg tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct
    10321 tctgcttttg ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt
    10381 cttctttttt gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt
    10441 ctagaaagat cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa
    10501 aaataatgtt catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat
    10561 attgtaagtg atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc
    10621 ttcaactaac aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt
    10681 aggtgcaatt cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg
    10741 gaccatagta aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc
    10801 taacgtcgta tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt
    10861 tgttggcagc cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg
    10921 aacgacagat aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg
    10981 gttaagcgcg atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc
    11041 gatagccgcc tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag
    11101 gtcaacaatc ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa
    11161 agaaaaagaa aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc
    11221 gattcgttcg tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta
    11281 ttatactatt tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat
    11341 actatgacca taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag
    11401 ttcgagtact attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac
    11461 cgcaagatga gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc
    11521 tggcggtagc tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa
    11581 atccagttct gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc
    11641 caggaccaag cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga
    11701 tcatgtttcc cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt
    11761 ccagaatgtc taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg
    11821 atctgttcaa gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata
    11881 tcaagaaaac ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg
    11941 acaaattgga caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg
    12001 ataagtttat gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca
    12061 atgaaaacga gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag
    12121 aagacaggtg tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg
    12181 tgattgaatg cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta
    12241 tgacttgatt gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga
    12301 aaaagtagag gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga
    12361 tgccgtagaa ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat
    12421 ttttgctata tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt
    12481 cagttattcg aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt
    12541 taaaaacgta caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt
    12601 tgaagatgac aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca
    12661 ggagctattt gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag
    12721 tgtaaaatct gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga
    12781 actacttaca ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag
    12841 acaggataat gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca
    12901 aaaaaaaggc gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat
    12961 cgaacccctg catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc
    13021 cttttatcag tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat
    13081 catcccgaat atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg
    13141 ggcaaacggt cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc
    13201 tcaaaaatta gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca
    13261 agaaaaatgg ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa
    13321 accgaaaaaa ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc
    13381 acgtttgaca gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac
    13441 gatttttgat gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg
    13501 aaaagagatg cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt
    13561 tgaacatcag cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg
    13621 aaaaggaaac ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa
    13681 aggaaaagaa ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact
    13741 gatgaatctc gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg
    13801 taattttttg gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac
    13861 tcaatttttc caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa
    13921 attggatcat ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga
    13981 agaggaacca gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat
    14041 actcgaaaaa caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa
    14101 gcaactagaa aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac
    14161 gatattaata aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa
    14221 acgatggatt gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca
    14281 agaaataaaa gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact
    14341 agcgatgact acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg
    14401 acaaacaaaa atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat
    14461 tgtcgaatcc ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa
    14521 agaagaattg aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg
    14581 aattcttcaa gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg
    14641 acagttaaaa tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac
    14701 tgggtcgctc gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa
    14761 tagtcagcta ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa
    14821 gcagcaaatt gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg
    14881 atcaacggaa caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt
    14941 aaaagtagaa caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat
    15001 tgccaaaagt gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa
    15061 aatcatggca acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa
    15121 aaaggcattt cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca
    15181 atctttagat agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag
    15241 acaacaagca gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa
    15301 aaataatcag ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga
    15361 tacggatagt aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga
    15421 ctcgaatcaa ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac
    15481 gcttcggaca cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga
    15541 aaaggaacaa gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga
    15601 agaaatctgt gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac
    15661 ttcttatcat gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca
    15721 gctaactgag acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg
    15781 tatgatcgaa ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca
    15841 aaaaatctac gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct
    15901 tacgcaatta cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt
    15961 ggaacggtat gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct
    16021 ccaacgttta acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg
    16081 agggaaaacc ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc
    16141 gcataccctg tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc
    16201 cgatgtgatc cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg
    16261 atttgggtca ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga
    16321 aagtgaagga agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt
    16381 acaacaaata agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg
    16441 ataaaaaggg agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg
    16501 acgtcaatac ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt
    16561 gtggcatgca gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa
    16621 agagatagat tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt
    16681 tgctgtggaa ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc
    16741 aatgctagaa ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg
    16801 tctaaaaaca caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga
    16861 aacgattgag actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat
    16921 cggttcattg gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca
    16981 agcttacaca gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa
    17041 acgggcgttt caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac
    17101 gcatcaatta ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct
    17161 gcttctccca gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt
    17221 gcatctgcag ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc
    17281 tttatcattc aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt
    17341 tgattttttc tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac
    17401 gaaatatcaa caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc
    17461 agaagtagtg ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct
    17521 gtcagagatc ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc
    17581 cgtcgaattt attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt
    17641 cgtctatggc gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc
    17701 ttttcaggtg ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta
    17761 tcgctaccag aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata
    17821 tgcctttttc cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag
    17881 aaaattgcga gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga
    17941 gaccggttca tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga
    18001 tcatgtcctg caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt
    18061 tctttctttt gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga
    18121 atcgatacgt acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca
    18181 aaatcaattg gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga
    18241 tctcattcat ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag
    18301 agattatcaa aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg
    18361 tattttagcc gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc
    18421 ggaaaaagaa gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta
    18481 caactggcag gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg
    18541 gaataaaaaa gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta
    18601 tgccagctta agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct
    18661 tgatgaggca caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt
    18721 agctgtacct cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt
    18781 gtggtcattg tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga
    18841 acttgccgca gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca
    18901 aactgtgttg aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga
    18961 agaacaaaaa actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat
    19021 ggattctgaa gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg
    19081 acaaatttgc tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct
    19141 cgaacaagta aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt
    19201 ttctcaattt acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc
    19261 gacattttat ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt
    19321 taatgctggc gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa
    19381 tctcacggga gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga
    19441 acaagctgct ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat
    19501 gatctctgaa ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact
    19561 gttccaaaaa gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat
    19621 ccgcatgatt ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta
    19681 atgggtaaga aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga
    19741 ggctgggatt ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa
    19801 ggttatacaa agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata
    19861 tcgcaaattg gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag
    19921 ttagcacttt ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct
    19981 tctggaagca tatgaaattc tttctcagct agctgaaaaa tctctttttc tggtttttgg
    20041 aaacctgttg cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt
    20101 tgcttcagtt tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca
    20161 ttcttttcct tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca
    20221 agttcttcct cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag
    20281 tctaaatctt ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct
    20341 cctgccatta cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga
    20401 tgcatatctg catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc
    20461 tgctgatcat acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac
    20521 gcctttcggt tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt
    20581 ctaaaaaagc tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa
    20641 ggtttttata tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga
    20701 acaaattaat cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt
    20761 ttcgaaagat ttattttttt aatacagtct caatataact attcaaacat aaaataacgt
    20821 tatttgattt atttgtttat agtaaatttt atttagtaat cattgcttta aaatactacg
    20881 cttatataac agaga
//
LOCUS       DAHHPA010000049        20362 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.050, whole genome shotgun sequence.
ACCESSION   DAHHPA010000049 DAHHPA010000000
VERSION     DAHHPA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.050"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            292..1431
                     /locus_tag="NBH55_001629"
     CDS             292..1431
                     /locus_tag="NBH55_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCD1509617.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="NBH55_001630"
     CDS             complement(1498..2073)
                     /locus_tag="NBH55_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1509618.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="NBH55_001631"
     CDS             complement(2078..3052)
                     /locus_tag="NBH55_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCD1509619.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="NBH55_001632"
     CDS             3335..4303
                     /locus_tag="NBH55_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1509620.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="NBH55_001633"
     CDS             4416..4694
                     /locus_tag="NBH55_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1509621.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="NBH55_001634"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="NBH55_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCD1509622.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="NBH55_001635"
     CDS             5151..5906
                     /locus_tag="NBH55_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1509623.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="NBH55_001636"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="NBH55_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCD1509624.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEEMMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="NBH55_001637"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="NBH55_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCD1509625.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="NBH55_001638"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="NBH55_001638"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCD1509626.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPIADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="NBH55_001639"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="NBH55_001639"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCD1509627.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="NBH55_001640"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="NBH55_001640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCD1509628.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="NBH55_001641"
     CDS             10643..11185
                     /locus_tag="NBH55_001641"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCD1509629.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="NBH55_001642"
     CDS             11208..12764
                     /locus_tag="NBH55_001642"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCD1509630.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="NBH55_001643"
     CDS             12778..13680
                     /locus_tag="NBH55_001643"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCD1509631.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="NBH55_001644"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="NBH55_001644"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCD1509632.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="NBH55_001645"
     CDS             15123..15545
                     /locus_tag="NBH55_001645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCD1509633.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="NBH55_001646"
     CDS             16014..16244
                     /locus_tag="NBH55_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCD1509634.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="NBH55_001647"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="NBH55_001647"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD1509635.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="NBH55_001648"
     CDS             17655..17831
                     /locus_tag="NBH55_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCD1509636.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="NBH55_001649"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="NBH55_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCD1509637.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="NBH55_001650"
     CDS             18326..18709
                     /locus_tag="NBH55_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1509638.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="NBH55_001651"
     CDS             18878..19762
                     /locus_tag="NBH55_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD1509639.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="NBH55_001652"
     CDS             19876..20112
                     /locus_tag="NBH55_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCD1509640.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="NBH55_001653"
     tRNA            20200..20280
                     /locus_tag="NBH55_001653"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="NBH55_001654"
     tRNA            20287..20358
                     /locus_tag="NBH55_001654"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6762 a         3560 c         4423 g         5617 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaatga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgatagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga atggcgcaaa
    11401 acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag gccttgatcg
    11521 gacgggtagt taatccgcta ggtcagccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagca atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga gttcatgtac aacggtaaac atgtcttgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca gcaaaattaa
    12121 gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa atcttcttag
    12241 aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta tctgtatctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgcctag
    12361 acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac ttggatgcag
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaattgc
    12481 atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca catggattct
    12541 tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaagta
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg ttaagaaaac tggttatatc gtcatcactg cagatggcgg tttagtcggt
    13081 ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg ctcgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta ta
//
LOCUS       DAHHPA010000050        20343 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.051, whole genome shotgun sequence.
ACCESSION   DAHHPA010000050 DAHHPA010000000
VERSION     DAHHPA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.051"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            2..550
                     /gene="hslV"
                     /locus_tag="NBH55_001655"
     CDS             2..550
                     /gene="hslV"
                     /locus_tag="NBH55_001655"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCD1509641.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            564..1964
                     /gene="hslU"
                     /locus_tag="NBH55_001656"
     CDS             564..1964
                     /gene="hslU"
                     /locus_tag="NBH55_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCD1509642.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1982..2773
                     /gene="codY"
                     /locus_tag="NBH55_001657"
     CDS             1982..2773
                     /gene="codY"
                     /locus_tag="NBH55_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCD1509643.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2855..3730
                     /locus_tag="NBH55_001658"
     CDS             2855..3730
                     /locus_tag="NBH55_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCD1509644.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="NBH55_001659"
     CDS             complement(3862..4500)
                     /gene="plsY"
                     /locus_tag="NBH55_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCD1509645.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4627..5061
                     /locus_tag="NBH55_001660"
     CDS             4627..5061
                     /locus_tag="NBH55_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCD1509646.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5227..7272
                     /gene="parE"
                     /locus_tag="NBH55_001661"
     CDS             5227..7272
                     /gene="parE"
                     /locus_tag="NBH55_001661"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCD1509647.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7284..9734
                     /gene="parC"
                     /locus_tag="NBH55_001662"
     CDS             7284..9734
                     /gene="parC"
                     /locus_tag="NBH55_001662"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCD1509648.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10011..12242
                     /gene="pflB"
                     /locus_tag="NBH55_001663"
     CDS             10011..12242
                     /gene="pflB"
                     /locus_tag="NBH55_001663"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCD1509649.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12486..13247
                     /gene="pflA"
                     /locus_tag="NBH55_001664"
     CDS             12486..13247
                     /gene="pflA"
                     /locus_tag="NBH55_001664"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCD1509650.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13332..14258
                     /locus_tag="NBH55_001665"
     CDS             13332..14258
                     /locus_tag="NBH55_001665"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCD1509651.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14440..15333
                     /locus_tag="NBH55_001666"
     CDS             14440..15333
                     /locus_tag="NBH55_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCD1509652.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15467..15592
                     /locus_tag="NBH55_001667"
     CDS             15467..15592
                     /locus_tag="NBH55_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1509653.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15726..16358)
                     /locus_tag="NBH55_001668"
     CDS             complement(15726..16358)
                     /locus_tag="NBH55_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509654.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16506..17048)
                     /locus_tag="NBH55_001669"
     CDS             complement(16506..17048)
                     /locus_tag="NBH55_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCD1509655.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17183..17572
                     /locus_tag="NBH55_001670"
     CDS             17183..17572
                     /locus_tag="NBH55_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509656.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17576..18340
                     /locus_tag="NBH55_001671"
     CDS             17576..18340
                     /locus_tag="NBH55_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCD1509657.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18469..19416
                     /locus_tag="NBH55_001672"
     CDS             18469..19416
                     /locus_tag="NBH55_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1509658.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19546..20286
                     /locus_tag="NBH55_001673"
     CDS             19546..20286
                     /locus_tag="NBH55_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509659.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7138 a         3461 c         4300 g         5444 t
ORIGIN      
        1 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
       61 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      121 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      181 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      241 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      301 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      361 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      421 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
      481 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
      541 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
      601 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
      661 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
      721 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
      781 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
      841 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
      901 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
      961 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1021 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1081 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1141 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1201 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1261 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1321 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1381 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1441 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     1501 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     1561 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     1621 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     1681 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     1741 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     1801 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     1861 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     1921 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     1981 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2041 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2101 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2161 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2221 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2281 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2341 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2401 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     2461 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     2521 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     2581 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     2641 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     2701 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     2761 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     2821 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     2881 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     2941 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3001 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3061 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3121 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3181 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3241 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3301 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3361 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3421 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     3481 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     3541 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     3601 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     3661 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     3721 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     3781 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
     3841 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
     3901 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
     3961 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
     4021 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
     4081 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
     4141 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
     4201 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
     4261 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
     4321 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
     4381 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
     4441 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
     4501 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
     4561 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
     4621 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
     4681 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
     4741 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
     4801 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     4861 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
     4921 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     4981 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
     5041 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     5101 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     5161 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     5221 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     5281 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     5341 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     5401 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     5461 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     5521 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     5581 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
     5641 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
     5701 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
     5761 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     5821 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
     5881 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     5941 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
     6001 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
     6061 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
     6121 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     6181 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     6241 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     6301 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     6361 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     6421 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     6481 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     6541 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     6601 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     6661 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     6721 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     6781 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     6841 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     6901 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     6961 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     7021 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     7081 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     7141 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     7201 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     7261 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     7321 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     7381 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     7441 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     7501 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     7561 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     7621 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     7681 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     7741 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     7801 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
     7861 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     7921 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     7981 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     8041 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     8101 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     8161 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     8221 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     8281 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     8341 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     8401 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     8461 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     8521 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     8581 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     8641 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     8701 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     8761 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     8821 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     8881 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     8941 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     9001 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     9061 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     9121 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     9181 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     9241 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     9301 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     9361 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     9421 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
     9481 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
     9541 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
     9601 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
     9661 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
     9721 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
     9781 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
     9841 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
     9901 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
     9961 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    10021 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    10081 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    10141 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    10201 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    10261 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    10321 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
    10381 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
    10441 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    10501 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    10561 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    10621 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    10681 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    10741 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    10801 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    10861 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    10921 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    10981 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    11041 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    11101 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    11161 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    11221 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
    11281 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    11341 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    11401 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    11461 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    11521 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
    11581 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    11641 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    11701 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    11761 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    11821 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    11881 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    11941 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    12001 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    12061 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    12121 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    12181 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    12241 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    12301 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    12361 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    12421 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    12481 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    12541 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    12601 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    12661 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
    12721 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    12781 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    12841 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    12901 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    12961 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    13021 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    13081 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    13141 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    13201 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    13261 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    13321 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    13381 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    13441 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    13501 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    13561 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    13621 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    13681 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    13741 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    13801 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    13861 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    13921 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    13981 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    14041 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    14101 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    14161 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    14221 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    14281 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    14341 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    14401 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    14461 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    14521 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    14581 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    14641 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    14701 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    14761 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    14821 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    14881 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    14941 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    15001 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    15061 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    15121 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    15181 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    15241 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    15301 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    15361 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    15421 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    15481 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    15541 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    15601 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    15661 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    15721 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
    15781 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
    15841 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
    15901 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
    15961 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
    16021 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
    16081 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
    16141 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
    16201 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
    16261 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
    16321 aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa ttaaattgat
    16381 aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt aatcaaaata
    16441 ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt ttttaagttt
    16501 gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc tgaaatatat
    16561 tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag gcgatacagt
    16621 tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc ttccagcccc
    16681 attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg caaactttta
    16741 tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa tgccaatttg
    16801 tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata gacaaataaa
    16861 ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata aatcgtgatc
    16921 tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa catgaaacgt
    16981 aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa catttttgga
    17041 tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa aatcgtaagt
    17101 gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata atagaattat
    17161 aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg ccagccacgt
    17221 gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc caattgaggt
    17281 agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg tttttttctt
    17341 tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga atttttctat
    17401 ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt gcttatttcc
    17461 cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc agacaactgt
    17521 tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt aacaattgaa
    17581 tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg ttctgaaaga
    17641 attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga caagtaagga
    17701 gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga tcctgccaga
    17761 tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct atacattttg
    17821 ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt tagaaaaaag
    17881 aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa tgacaacatg
    17941 cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg gttcccctgc
    18001 acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt atgtcgtacc
    18061 aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat tgcagccgga
    18121 aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa aaatccctta
    18181 tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa aagaaattgt
    18241 ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg aagaagcttc
    18301 cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga aaatcactca
    18361 tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt taggaaaatt
    18421 tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat gcccacttta
    18481 gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt catcaatcat
    18541 ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc tgaactggat
    18601 taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat catcaaatta
    18661 tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct gatgggaatc
    18721 gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga tgccttcgac
    18781 gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt cgattggatc
    18841 gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata tgattatgta
    18901 gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga aagagattat
    18961 caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc aagggaaact
    19021 ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg gtttccaaat
    19081 cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac agataaaact
    19141 tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat tataaatgct
    19201 ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt tttagaccaa
    19261 atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg tgaagtatgt
    19321 ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta tcgtcgtttt
    19381 tttcctgaac tagttatcag agagacaacg aattaagtct tattactata tatatattta
    19441 tgaaattcat cgctattttc ttaaaataat cagcgcattg ctactgaaaa caatgaatct
    19501 gtgctattat tgagaaaagt tccatttttg agaaggatgg tttgaatgaa agaaatcaga
    19561 cgaaaacatt tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt aaccaacgac
    19621 aagaagcgag tattatttga cggacaagaa gatcttttga ctgaacagaa tcagtccccg
    19681 tttgaacaga tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat ccgtcgcaag
    19741 cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa
    19801 ggaaaattac cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa
    19861 aaactttttg gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag
    19921 catactacac gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct
    19981 aaatatgtac ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat
    20041 ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg
    20101 ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg
    20161 ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa
    20221 cgctcattac aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt
    20281 aaataagtga tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt
    20341 cct
//
LOCUS       DAHHPA010000051        19322 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.052, whole genome shotgun sequence.
ACCESSION   DAHHPA010000051 DAHHPA010000000
VERSION     DAHHPA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.052"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..456)
                     /locus_tag="NBH55_001674"
     CDS             complement(<1..456)
                     /locus_tag="NBH55_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD1509660.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="NBH55_001675"
     CDS             complement(561..1424)
                     /locus_tag="NBH55_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1509661.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="NBH55_001676"
     CDS             complement(1620..3719)
                     /locus_tag="NBH55_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCD1509662.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="NBH55_001677"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="NBH55_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCD1509663.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="NBH55_001678"
     CDS             complement(5072..6913)
                     /locus_tag="NBH55_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCD1509664.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9410)
                     /locus_tag="NBH55_001679"
     CDS             complement(7095..9410)
                     /locus_tag="NBH55_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCD1509665.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="NBH55_001680"
     CDS             complement(9595..10455)
                     /locus_tag="NBH55_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCD1509666.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="NBH55_001681"
     CDS             complement(10514..11035)
                     /locus_tag="NBH55_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCD1509667.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11313..12074
                     /locus_tag="NBH55_001682"
     CDS             11313..12074
                     /locus_tag="NBH55_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1509668.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="NBH55_001683"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="NBH55_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCD1509669.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="NBH55_001684"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="NBH55_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD1509670.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="NBH55_001685"
     CDS             16434..16790
                     /locus_tag="NBH55_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD1509671.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17031..18035)
                     /locus_tag="NBH55_001686"
     CDS             complement(17031..18035)
                     /locus_tag="NBH55_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCD1509672.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18201..19052
                     /locus_tag="NBH55_001687"
     CDS             18201..19052
                     /locus_tag="NBH55_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1509673.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19069..19314
                     /locus_tag="NBH55_001688"
     CDS             19069..19314
                     /locus_tag="NBH55_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509674.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         5388 a         3991 c         3379 g         6564 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gtataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa
    16861 ggagttgctt ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt
    16921 acttttgtct cacgctcttt ttttgttata ttcgtctttt ccgatccttc tcttgacaca
    16981 attcatttaa ctgatcactg gtacttgaac acctaatatt ctagtcattt ttataaacga
    17041 ttcaatactt catccacatt tttgatcatc acggatatcg taccatccgg attattcaca
    17101 aattcaatca aatctgggtt gcgatataca tctactggta cgatcagttc gatcccgttt
    17161 gatagcttga atttttgttt tccaaatttt ttctcagaaa tttcttgtac ttcttttaca
    17221 ggaggtgctt ctgggacaaa tccttgctct tgtacttctt ctttaaaagc catttttgcc
    17281 gtgatgtttt ccttgaagac tttttccgca atcatctcat gatccaaccg acctttttct
    17341 tctatcgtat catagacagc ttctttcaaa tcagccatga tatcaaattc ttctgtctca
    17401 aatttttttc caagatgctt ggctactttt tggatcactt ttacattttc ttctaaagaa
    17461 ggtgcaggaa cactttcgat cactcttcca gaaaaataaa gctctttttt gccagaaaac
    17521 tcatattttt tttcaattaa ttcgtaggtc caatcagtca aattcacagc gaatgcttca
    17581 tctgcttttt gtgttttcgc ccctagaatt gctcgattca aaatcaagcg gttgtcgatc
    17641 cctgcttcgt ctgcttcaac aaagtgagtg taagcctcac gataattgac tttcaataaa
    17701 gataagtaca tcactgtatc taattcatac aagacaacaa atacatctgc actaggtgct
    17761 tcttcacttt cttggtagat atcgaaccac cgctgaacga ttgcttcact tgcttccaca
    17821 aaattttctt gagcgatttt aagtgattca acgatcatgc tttcttctgc tagttgtcca
    17881 gtttttgttt gagcagaaga gattttttgg atcttctttt gaagaaaatc acgaatatat
    17941 tcttttgtca gatccagctc tttttgtgaa aagactggag aaccagtttc tctgtcaata
    18001 atatgcagaa ttgctttttt taaatagata tccattttct cattcccttc tgtcttagtt
    18061 tacggaaaaa aaatcaaaaa taccagaatc aaaatcattt tcttttaaac aaaaatttca
    18121 agagtagaaa caaaatggat tctttttttc gaaaaatgcg ctacacttat agttgaacga
    18181 ataaaaaagg agctgaaagt atgaattcat taacaagcac ttatcgtcta tcaaatggtt
    18241 acgaaatccc ggtagtagga ttcggcacat ggcaaacacc tgatggggat gtcgctgttt
    18301 cctctgttaa agaagcgctg gctgcaggtt atcgccatat cgatactgcc caaggataca
    18361 agaatgaaga aagcgtcggc caagcgatta aggaaagtgg aattcctaga gaagagatat
    18421 tcttgacaac aaaattatgg aatgcaaacc acagctatga acttgtgatg tcttcttttg
    18481 aagaatcatt gaagaagctc caaacagatt atttagatct gtttttgatc cactggccaa
    18541 accctgttgc ttttcgtgat aattgggaac aagcaaatgc agatacatgg cgagcatttg
    18601 aagaactgta tgaagcaggt aaaatcaaag caatcggtgt gagcaatttc ttaccccacc
    18661 atctagatac actagcaaaa acagcaaaaa ttatgccgat ggtcaatcaa gttttccttg
    18721 caccaggcga attacagaca actgtagttg agtatgcaaa aaaacatgat atgatcctcg
    18781 aagcatatag tccattaggc actggtaaaa tcttcgatgt gcctgaaatg aaacaaatcg
    18841 ctgaagcaca tgacaagacg attgcacaag ttgctttgcg ttggtcgctg caacacgaat
    18901 tccttcctct tcctaagtca gtgacaccaa gcagaatcaa agaaaatact gaattatttg
    18961 attttgaatt aacagaagaa gaaatgaaac aaatcgatca gttagatggt gtagtcggca
    19021 aagcaaaaga cccagatact acccaatttt aagtcgaaag gtgattttat ggatccaatc
    19081 gaacgcttga attctctctc ggaagaagtt acccagactt ttcattccga ttttgtcttt
    19141 ttgatcgatg cagagaaaat ccaacatttt cctgcaagaa actggacaca tgatcaaatc
    19201 atcgaagaac tgaaaaaacg gttcgatcat tcgctaatgg tcaaaccctg gcacgaacat
    19261 gaagtcattt attcacctga actacctgtt tttgccttga taccgaaaaa atagaaagaa
    19321 tg
//
LOCUS       DAHHPA010000052        19238 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.053, whole genome shotgun sequence.
ACCESSION   DAHHPA010000052 DAHHPA010000000
VERSION     DAHHPA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.053"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            211..525
                     /locus_tag="NBH55_001689"
     CDS             211..525
                     /locus_tag="NBH55_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCD1509675.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            503..2197
                     /locus_tag="NBH55_001690"
     CDS             503..2197
                     /locus_tag="NBH55_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCD1509676.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2217..3395
                     /locus_tag="NBH55_001691"
     CDS             2217..3395
                     /locus_tag="NBH55_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1509677.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            3358..4044
                     /locus_tag="NBH55_001692"
     CDS             3358..4044
                     /locus_tag="NBH55_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCD1509678.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            4044..5204
                     /locus_tag="NBH55_001693"
     CDS             4044..5204
                     /locus_tag="NBH55_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCD1509679.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            5214..6089
                     /locus_tag="NBH55_001694"
     CDS             5214..6089
                     /locus_tag="NBH55_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509680.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            6086..6397
                     /locus_tag="NBH55_001695"
     CDS             6086..6397
                     /locus_tag="NBH55_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509681.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            6387..6740
                     /locus_tag="NBH55_001696"
     CDS             6387..6740
                     /locus_tag="NBH55_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCD1509682.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            6730..7131
                     /locus_tag="NBH55_001697"
     CDS             6730..7131
                     /locus_tag="NBH55_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509683.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            7124..7528
                     /locus_tag="NBH55_001698"
     CDS             7124..7528
                     /locus_tag="NBH55_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509684.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            7540..8148
                     /locus_tag="NBH55_001699"
     CDS             7540..8148
                     /locus_tag="NBH55_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1509685.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNATNAISTDCNGQLDGETEM
                     QRIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSV
                     GEDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            8168..8530
                     /locus_tag="NBH55_001700"
     CDS             8168..8530
                     /locus_tag="NBH55_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509686.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            8533..8715
                     /locus_tag="NBH55_001701"
     CDS             8533..8715
                     /locus_tag="NBH55_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509687.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            8732..12163
                     /locus_tag="NBH55_001702"
     CDS             8732..12163
                     /locus_tag="NBH55_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD1509688.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            12214..12951
                     /locus_tag="NBH55_001703"
     CDS             12214..12951
                     /locus_tag="NBH55_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1509689.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            12961..15252
                     /locus_tag="NBH55_001704"
     CDS             12961..15252
                     /locus_tag="NBH55_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1509690.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            15276..17402
                     /locus_tag="NBH55_001705"
     CDS             15276..17402
                     /locus_tag="NBH55_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509691.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            17419..17568
                     /locus_tag="NBH55_001706"
     CDS             17419..17568
                     /locus_tag="NBH55_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509692.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            17565..18011
                     /locus_tag="NBH55_001707"
     CDS             17565..18011
                     /locus_tag="NBH55_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509693.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            18013..18150
                     /locus_tag="NBH55_001708"
     CDS             18013..18150
                     /locus_tag="NBH55_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCD1509694.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            18188..18481
                     /locus_tag="NBH55_001709"
     CDS             18188..18481
                     /locus_tag="NBH55_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509695.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            18478..18702
                     /locus_tag="NBH55_001710"
     CDS             18478..18702
                     /locus_tag="NBH55_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCD1509696.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            18699..>19238
                     /locus_tag="NBH55_001711"
     CDS             18699..>19238
                     /locus_tag="NBH55_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD1509697.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLS"
BASE COUNT         6793 a         2902 c         4022 g         5521 t
ORIGIN      
        1 aataaagata ataacgtcga aagaataaaa aatgtttttg gatatagtat tgaagagatt
       61 aattattaca ttgacttcgc tgaagctttc attccgatgg tgagataacc cccccttaaa
      121 ataaatcgca aattttttgg gggtgatgaa acggaggggg ctgtcaggaa aagagatttt
      181 ttcgaacttt atcatgaaag gagggctaaa atgtttaaaa acgaattgtc tcaaaatcgc
      241 tacagagaaa aattacgccg ctctttaata agccaattgg aaagtcagaa aacaaatatt
      301 gagccattct tagataatgt tgatcgttat atcagtttat gggaaacggc gatatcactg
      361 gaagaagata tatccgagaa cggcattaga ctggagaatg gtaaaaagaa tgaatcagta
      421 gcgttgcttg tttctgtcaa caaacaaatg ggattgatgt tggataaact tgccattact
      481 cctgaattgg taggtgaagc aaatgaatca attcctgagt tataagcata ttgaaaattg
      541 gttcaaagct atagaagaag gcactatcaa ggtatgcaaa gagcaattat tgctaaaaaa
      601 ttatctagaa gaaagagtct ttactagaga agatatttac ttcgataagc agatggtaga
      661 ggattcaatc aatataccag cacaatactt tccattcgaa ttaattccgt gggaaaaatt
      721 tctacaatgt tttatttatg gtgttcgatg gaaaaaagat aaaacactag tgttcaatag
      781 atatctttca ttaatgggac gtggtaatgg taaaactggt tttgcttctt ggaacaactt
      841 ctttctacta accgctaaac acggtattaa aaattatgat attgatatct atgccaataa
      901 tgaaagccaa gcaaagacta gttttgatga tgtatttaaa gtaattaaag atcatcctga
      961 tttagataaa aaagtattta aagctacgaa ggaagttatt caaaatatcg ctacaaacag
     1021 caaacttcgt tataacacgg caaatgctag aacaaaagat gggaagcgac caggtgcaaa
     1081 ccgctttgat gaaattcacg aaaatgaaga ttattcaatg ataaatgtgg ctacttctgg
     1141 tggtggtaaa attcgagatt atagagaatt ttatgataca actaatggtc atgttcgtgg
     1201 tggtccgctt gatgacatta tagaagaatc aaaaatgatt ctttctggag aacttggaat
     1261 tgacaaggat ggagcagaat tttctagttt gtttccattt atttgtcgct tggataacga
     1321 taatgaagtt gatgatcccg acatgtggga aaaagcttgt ccaactatta attacaatgc
     1381 agatttaaaa cggaaaatgt ttcaagaata ctctcaaatg caacgtaatg ctggtttaag
     1441 acttacgttc atgaccaaac gaatgaacag acctatggaa gatacacgat ttgctgttgc
     1501 ttcatatgat gatgttctgc atacgaaaga aaaagaattt cctgaaaaaa tggatgaagt
     1561 gataggaaca gtcgattttg ctgatagacg agattttgcc agcgttgggt tgctaggaaa
     1621 atacgataaa gatgtttatt ttacacaaca tacttttatc cacgaatcag cccttcgatt
     1681 acaaaacatc aaacgagagg ttatagatat ttctatagat caaggaaaat cacagatcgt
     1741 tcatggaaaa aatatagaag ctgattatat tgtaggttgg tttcttgaaa tgagtaataa
     1801 atattatatt aaaaaaatcg ctatggatat gtaccgtgca aaaatattga agcccgcttt
     1861 agaagaagca ggttttactg tggaaattgt tcgaagcgga tctgttacac atggtatgtt
     1921 aaaagatctg gttgatgacc tttttattaa tcaacgttta ttttttggtg acgatgcgat
     1981 tatgcgttgg tattgcatga atgtatatga agagcatatt tctaatggaa atatacgcta
     2041 tgaaaaaata gaacctgaaa ctagaaaaac ggatggcttt ttttcattcc ttcatggttt
     2101 gaatttttta gatgatattt atgattctgc tcctgtaaca gtcacaaata gctcagtaga
     2161 aaatacagga actggattta ctcctctagt attctaactt gaaaggaggt gagaaagtgg
     2221 ggatttttca aaaggcggta ggatacttca caaaaaaagc aacggttcct ttagaagaat
     2281 acttttgtaa attgcaagtt gattttgtgt atcgaaaatt tgcgattgaa acttgtattg
     2341 atttgattgc aaatgcgatg agtaaagcgg aattcaagtc atatgaagat ggaaaaaata
     2401 aaaagaatga tctttactat aggctgaatg tagctcctaa taagaaaaat aatgcaacag
     2461 aatttagaaa aaaactgatc aggagattaa tattctacaa tgaagtattg atcgtttctc
     2521 cgtctaataa ttctagcgaa atatttattg cggatagttg ggatgtcaca gaatatgcat
     2581 tgaaagatga tgtgttttct caagtgcaaa ttaacaacat agtccttgat agagaatttc
     2641 tagaaagtga tgttatctat ataaaatacg cagatcaaca aattaggcaa ctagtcgatg
     2701 cgtattatca agcgtatggg aaactcattt ctagtgccat gaatgtttac aagcgctcta
     2761 acgctcgtag atacgtactg aaagggaatt tattccgacc gcaagacaat acaacacaag
     2821 atcaaatcaa taaaatgatg acatcacaat ttaaggcttt tatggaagct gataatgcag
     2881 gtgcggtatt tcaattacaa aatgagtaca cattagaaga tttcagcgga aacttccaaa
     2941 gcaattcaag agatataaaa aacttaatag acgacatctt tgagatgaca gcagcagcgt
     3001 ttcacgttcc gaaaaaccta ctaaagggag acatgagtgg gttatcggat caagtggacg
     3061 cttttttaat gttcgaaatc ataccgattg ctgaacttat tcaggatgcg tttaacgcta
     3121 gtctctatga agtagaagaa tacttgtcag ggaattttgt acgtgtggat acaactatga
     3181 tcaagattac tagcttcaaa gatttggttg acgctattga tgtaggcatt agaaatggag
     3241 tatttacaat caacgaagga agagagcgcg ttggaaatga tcgctctgat aaggcgatgg
     3301 cagatgaaat atttataact aaaaacaatc aacaagtatc gaaaggaggt gaggcgaatg
     3361 acgacaatga aaacatttct agcagtaaag aatgaaggcg cagtaccgca aatttttatt
     3421 cagggattta ttggttctag ttggttcttt gaagggaata ctgacaaggg aatcaaaaat
     3481 attttggata gtctaggtga tcaagaagaa attgaagtag taattaattc aaacggtgga
     3541 gacgtatttc aagggattgc tattgggaac ttacttaagt caaataaagc aaaagttaac
     3601 gttgtgatta acggcttagc cgctagtgct gcttcaatta tcgcaatggc tggcgatact
     3661 ataaaaatct acaacaatgc acaattgatg attcaccgcg cttccacata cggagaagga
     3721 aatgtcgatg acttccgtac gattgctgac caactggaat caattgataa atcggtaaag
     3781 gcttcatata aaacacgatt caatggcaca gatgaagcat tgcaagaact tcttgaaaaa
     3841 gaatcgttta tggatgcaga aacagctttg agttatggat tggtcgatga aattatcgat
     3901 gcagaaaata gctcaggtac tgaagctaaa aaagaacaaa gcgttgaaga aattttgaat
     3961 gacgttaaag aaaaaagagc agaaaaaatt gctgcattta cagcagcatt aaataaaaca
     4021 tttggacaag gagatgtaaa ataatgacag ttaaaaattt aaaaggtgta acagctgcaa
     4081 gcgaccaatt gatgaaagct tttaaagatg gtaacgaaga atcttttagc gcagctatgg
     4141 taagcttatc taaggaaatt caggataaaa ttttagaaga agcaacagca aaaaatcaag
     4201 atcaattagt attaatgaac cgtggtcagc gtgtattaac tacacaagaa acaaaattct
     4261 ataacgaagt agtgaaaaac gaaggttttg caggggtcga agaattagtg ccagctactg
     4321 tatttgaacg tgtatttgaa gatttagaac aatctcatcc actattgcaa aaaattactt
     4381 ttgttaacac aactggtgta acagaatgga ttgtgtcacg tggagtcaat ccagcatggt
     4441 ggggtaaact ttgcgaagct gttaaaaaag ttttagataa tggctttgat gtaattaaca
     4501 tgaagcagtt caagctatca ggttatattc ctgtatgtaa ggcaatgctt gatttaggtc
     4561 cagtatggtt agatcgttat gtccgtactg ttttagtaga atcattgaga attgcattag
     4621 aacaagcaat tgttgatggt actggtaaag atatgccagt cggaatgatg cgtgacatga
     4681 gcaaacaaac tagcggagaa tatgctgaaa aaaaagcaga acctattaca gctttagatg
     4741 ctgtaactat gggcggtttg atggcgcgac tatcaaaatt caatatcgaa ggtgtgaatg
     4801 atccgattta tcgtaatgtg aatccttctg atgtggtcct aattgtgaat ccaactgatt
     4861 actggtctaa agtattccca gctaagactg tactaactgc taatggagaa tacgtacaag
     4921 tattgccagt accagtttca gatttgcagt caacggctgt gccagaagga aaagcagtta
     4981 ttggggtagc ctcagattac ttcatgggtg taggatctac actaaaaatt gaagcttcag
     5041 atgaatacca ttttgttgaa gacgaacgca tttatctagc taaacaatat gcaaacggtc
     5101 aacctaaacg taacgatagt ttcattgtat tagatattag cgctttggga actactacta
     5161 caactacaaa accaacaacc acaacaacta caacacaagc gtaggtgatc agaatgaagt
     5221 atattctttg tcagccggca atcaatcggt ttaaatggga gcttgaagtt tgtttaacta
     5281 atctgaagaa actaggaatc aaagatatcg tattgctttt cagcagacac gatgatcaga
     5341 ttcctatttt ttttgagaag gaatatggtg ttgaagttca tgtgtacgat gatctgcggg
     5401 acgacaaaga gtatattcct tcgattaaac catatttatg gtggaaatat ttagaagaag
     5461 atcattcgcg tgaggacgac cgatatttct atatcgattc ggatgtcatt ttcaataaaa
     5521 gaattaattt gcgcaaattg ccttctaaag atgatgtttg gtattgtagc gactgctgta
     5581 gttatctaag tcttgattat attagaagct gtgaaaacgg agaaaatatt ctaaaagata
     5641 tggcaaacat tgtaaatgtt acagtagaat ctttggaaac tataaacact aattcaggag
     5701 gcgcacagtg ggttattaac cgccctaaag cgaattattg gaaaaaggtt tatctggatt
     5761 ctaatcggct atatcgctac cttagagggc aaaaaacaaa tatacaaatc tggacagccg
     5821 agatgtgggc acagctttgg aacatgatgt atttcaatat tggtcctaaa gttcacgagg
     5881 aattagactt ttgttttgct actgatccaa tagaaaaagt taaagaagta aaaatcttac
     5941 acaatgctgg agtaacaaca aacgatgaag atttattttt caaagggaga tacgtgactt
     6001 ctacgccttt tgatgaagat ttatcatttg taaacaagaa aaaatgctct tacgcatatg
     6061 ttaaagcaat taaggcggtg gttagatgac gcctgaacaa gtgactgaag aattgctaat
     6121 agctgtgaag gataatattt acgttacctg gaacgaagaa gatgagtcaa ttaaaaagat
     6181 gatagctaaa aatgctgttt atcttcaaag taaagtgagt acaacacttt ctttttctcc
     6241 tgaaagctta gaatacggat tgctaatcga aagatgtaga tacgactgga atcgtgcttt
     6301 agatgagttt gaacaaaatt tcgctagtga gttattaggt ttcattcaac attatgcgct
     6361 acaagaatat attgcaggtg atgtgaatgg cgaataatcg tagactcgaa gaaacattca
     6421 acgatggttg gttaaagatt ttgacgcaaa ctaccaaaag aaatgaacta ggaaaaaaga
     6481 ttggtgtaga agatacagaa atcacttctt taaaatttag aaatctttcc atgagagata
     6541 gtgatataac agctatggat gcgatgggat cgaaattaac taagaaagta aagactccat
     6601 ttcatccaat cgccaagaaa tttaataaag atcaatattt tatcgtaatc gatagtatgc
     6661 gttacaacgt tatctatgcc gattacgata atttttatat ctatttttat cttgaaagtg
     6721 tgggtgaata tggtgattga taattctaaa gaaaaagaac gtttaaataa gcaaatttct
     6781 gctatcaaaa cttccttaga agagcatttt ggcctcaaac tctttcaaga ctccgtaggc
     6841 gaggatgagc tacctgatga ttttaattac ttcattctcg aaacaggaga aatagaaatg
     6901 atcactgagc caaaatatag cgtgggtcaa aatctatatc taactttcta ttcagaaaat
     6961 agagaagatt taacaggaga ctcactagat attatttcat tgattcaaaa tcgttcgatt
     7021 cgttttcaga gaatggatcc caatcattta aaactagaga accaagatcg ctatatcgat
     7081 caattggtat ttacgtttag acgattattg aagagtgatt gtcatggcta aaaatagttg
     7141 ggagctaaaa ataaatggac atgatgaact tcttgtgcgg atggaacgct attcaagcga
     7201 gagcgaacga ctgattaacg aagcattgaa atcaaaaggt tcggctattg cagtggatag
     7261 gattacagaa aaaattcctg tttctgaagc agatttaaga agagggcacc aacacgcaaa
     7321 aaatagtcgt ccacttaaga ctcaatacat taatttgggt ttcatcatta gacctacaag
     7381 aaaatttgag tatttaaaat atcctgattt ggggataggt acttctaaaa gaaatcagcc
     7441 ggacgaattt atgagaagag gattaggtct tgcacttgat ccaattacag aacttctgat
     7501 tcgtcaattc gataaattaa ataaatagga ggaacaacaa tggctaaaac aaaaactgta
     7561 gtaacaacgt tcgataacgt aagtatcaaa cgaattgctt ttaattttaa gaatgcaaca
     7621 aatgcaattt caactgattg taatggacaa ttagatggcg aaacagaaat gcagagaatt
     7681 gtaaaaaaat gcggttcaac agaagtaaaa tcgaaatcta aaccaatcaa tatgacggta
     7741 acaattactg cacatgtacc gatggaagtt tatcgacgct tcaatgggtt gaaacaagat
     7801 gaacgtatta aatcgggaat ttactcttat ggtcctgatt ccgtagggga agatttctct
     7861 cttgctgcag agatcgtgga tgatttcgaa gaaaaaaata agctgattgg tatgttagca
     7921 tgcacttcga atacaggatt aacattctct attgaaaatg gtgcggatga agtagctgcg
     7981 ttagaactag aaacaaaagt tatgcaagat gaatttggta aattttatca tgaagcaatt
     8041 gttgcagaac ttgaagaaga cttaacagat caatggatga cgaatctatc tgctgatgtg
     8101 attaaaaaaa cttcagttgt gacaactacg gccactcaat cacagtaaaa aaaacggagg
     8161 tagcgaaatg aacgaagatt actcaaaaat tgaactaaac gatggaacaa ttttgaattt
     8221 agaacctaaa ctgaatatca agaaattatt gatgatcaat agagatttta acacagacga
     8281 gtttgcaaaa atgactgtgg gaaaaggatc catggatatt tctgttattc aaggtgcaaa
     8341 ggctgtgtat attgcttacc gccaagcgaa catgactgat tatatttcat tcgatgaatt
     8401 tatcgataaa tgggattttg atatggctac tgccagctat atttatcaat tgatgatgtt
     8461 caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa gcaaataagg aaaaaaagct
     8521 tcaaaagtaa aaatgccaaa gctcttagtt gaaacgtggg tcgatgtcta ttcgatgttg
     8581 accgacgttt tttctatgcc ttcagatttg gttttaagcg atatctgttt agatgacatt
     8641 ttgcaaatgg cttacaacaa gagtgcttat gaaggatgga aaaactatgc aataaaccaa
     8701 tcccagaaaa actaaagaaa ggaggtaaaa aatggctaaa aagagaacag aagcagaagt
     8761 aacattcata gctaacgatg acggattgaa atctacgtta aaagaaatca gtgctgaatt
     8821 aactaaaaat agagcagaat taaaactaga acaagctcaa ttacaacaga ctggttctga
     8881 atcagacaag ttaggaagta aattatcttc tttagaaaag cagtatgaat tacaaagtca
     8941 aaaagttgaa gtaactagcc aacgtttagc caatgccaaa aaatattatg gagaaaattc
     9001 caccgaagtt cagaaacttg agagagaact gattaaccaa caaacagcgc aacaacgttt
     9061 gtcaaacgaa attgataaaa cgagtaatgc actagctcaa gcaaaaggcg aaatacagac
     9121 gtacgagtct acaatgcaac agttggatag tgaacaaaaa aatgttcaag ctagtgcttc
     9181 tctgattgaa tccgaataca aaaaatggca agcaactgct ggtcaatcag cttctgaatc
     9241 cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa tctgaaaatg cagagaaaac
     9301 gatagatatc ctgagacgac agttagaagc tacacagtct gagtttggcg ctacatccac
     9361 agaagcaatg cagatggagg cgaagcttaa tgatgctgaa cgtgaatttg aagagttagg
     9421 acaagctgct aaaaatgtag atacaactaa cttggacgat atcggaagca aaatagatat
     9481 gaataatcta atggaagctt ctgacgtttt aagcgacatt ggcgataagc ttacagaatt
     9541 agggaaacaa gcagtggact ctgctaatag tgtaggtagt tcccagagta aaatacaagc
     9601 taattttggt ttgactaaac aagaggctga agaattaacg aatgtagcca gagacattta
     9661 ttataaaggt tttggagaat cgttagatca gtccacagat gcattgattt tggtaaagcg
     9721 taatttaggc gatttaaata atcaagattt acaaaatatc acggaacaag ctatggtcct
     9781 agaaaacacc atgggcgctg atatggatga aacgttacgt ggtgtaaatg gcttaatggt
     9841 caatttcggc ttgagcgctc aagatgcaat ggatttaatg gtttcgggta ctcaaaacgg
     9901 tttagataaa acgcacgaat taggcgacaa tatggcagaa tatagccaat tatggagtca
     9961 aatgggatat tcagctgatg aaacgttcgg aatgcttcaa aatggtttag atgcgggtgc
    10021 ttataacctt gataaagtca atgacttagt taaggaaatg ggaatatcgt taacagatgg
    10081 tcgatttgag caaaacatgg atatgtttag tgaaagtact agaaaagctt ttgaagagtg
    10141 gaaaaatggc ggaggaacac aaaaagacgt tattaattcc atgattcaag attttagcaa
    10201 tatggatggt caatacgacc aattaaataa agcttctaca atttggtctg cacttggcga
    10261 agataacgcg atgaaagttg tccaatcttt gactgatgtt aaccatacat ttgatgatgt
    10321 tagtggatct gcacaaaaaa tgaatgaaga ttctactact ccgttacaag aattaaacgg
    10381 aaaaatagct gaattaaagg attcattagc tcctataggc aacacaatca tagatgcact
    10441 cgaaccagta attgattttc taggaaagat ggctgatgcg tttaataatc ttccacaacc
    10501 agtacaggat ttcattgttg ctataggtgg tcttacagca gcatttggca tactgttacc
    10561 ggcaatacta gccgtgtcat atttatttgg tccgatgatg cttataattg gaggaattat
    10621 agctgttata gcaggcgtta ttgtggcaat taagaactgg ggtgcaatta ctgactggtt
    10681 tagtggttta tggaaaaaat ttactgattg gttgggtgat acttgggaaa gtataaaaga
    10741 cggagcctca tcagtttggg atggggttaa agaaacctgg tctggatttg tagattgggt
    10801 tcaagatatt tggcaaggag tttctgattg gtttggagag ctatggagcg gattagttga
    10861 aggagcttcc aacatctggc aaggagtcca agaaacttgg caaacattcg ttgattgggt
    10921 ttcaaatatt tggaacggag tcaaagaagt atggtcgatt atttgggcag acattgtagg
    10981 aattgttcaa ataccatgga cattaataac gtcattgatt caagctggta ttaatattat
    11041 cgtgggtatt tttgatgtag ctggacagtt attgggcgca gcttggcaag ctgtttggac
    11101 acctatttct gatttcctta aaaatacttg ggatactatg acacaatgga taagtatcgc
    11161 ttggaatggg attgtaacta cattccatac tatatttgat ccagtagtgg catggtggaa
    11221 tggtatatgg acagctatta gtactacggc ttcaaatatt tggaattcaa ttagtgcaac
    11281 agcttctagt atttggaaca gtatcaagaa tacaatcact agcttggtac aagcagctgc
    11341 tacagtaatt caaaatattt ggtcaactgt atctagttgg ttaggtggaa tttggaattc
    11401 aatcagctct acagcatcaa atatctggaa tagcgtgact agtagtataa gcaatgctat
    11461 aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt atatcttcgt ggattagcgg
    11521 aatttggaac ggtatcaaaa atactgcttt gaatctttgg aatggaatta caagcactat
    11581 tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc ggttggtcca atctaacagg
    11641 tattgtttcc gacatattca ataatgttaa aagtacaatt gctaacatat gggaaggcat
    11701 caaaaagact gttagcgctc caattgattg gattagagac aaaatcagtg gcatttttga
    11761 taatttgaat atttctatac cacatattcc gttaccggaa tttatcatgg aaggcagctt
    11821 taacccgcta aaaggtcaaa tcccccattt gcgtgtaaaa tggcatgcta aaggaggtat
    11881 ctttactaaa ccaactttac taggtggaat gaacggtgtc ggtgaagcag gacctgaagc
    11941 agttttacct ttgaaaagat ctgttttgca agaaattggt gatcgtatct tgagtagcac
    12001 atcagtttca tctagggcac aaacgattca acctgtgaat aactacgaat tcaatttcac
    12061 aattgatggt aacgcagatg aggttactat gaagcaaaca actcaacaaa tcattgatag
    12121 cattacaaaa attcaaaatg ataatgcttc ggcatggcgt taaacaggag agtatttctc
    12181 ctgttttttt agtattaaaa aggatgtgaa aaaatgactg attgtataca ttctataatc
    12241 gatggatttc ctgattattt gcataaattg gctttagctg aaagaccaac cataccttct
    12301 ccaaaaagac agagagttga aacttctgtt ttaggtaggt taggtggctt agtacaagat
    12361 tactcgtttg aagatatgtc gtttacattg cactataact atttagagga tgtggaagac
    12421 catcaagcgt tcaagcaatc gttttatatc atgcgtcatt ggttaaacta tgcaaagaaa
    12481 ttagaattct ctgacgatcc caacgtctat tacgttatcc agactatcga tattggggat
    12541 gcagaaaacg atattgttga atggggagag ttcgatgtaa atattactgc gaaaccattc
    12601 gcaagagttc aagaagatgt acctataacc gtagataaac cacagtcatt taacttgctg
    12661 aataatagtt tagaagaaag ttttccaaag attatcatca ctccttcagc tacttcatgc
    12721 cagttcatct taaatgatta tgtgtttagt ttcgaaggct tagtagcagg aactgatgta
    12781 gtcattgata gtgatttgat gctttgctac gaagagcaat cggacggaga tattttagat
    12841 cggtccaaca aaatgaagac catgcaatat ccgacattgc aagtggatat caatcatttt
    12901 aattgcactg gtttgagcaa aatacaaatt tatcgtaacg ggttaaggta ggtgaaatag
    12961 ataatcgata atttaataac tatttacgat aaaaatgacg cgaataattt agctgaacat
    13021 ttatatgata cgcaaggttt aggcgctttg tcagactggt taacagctac tgttagcaat
    13081 aaactaaacg gagccgagat atttcagggt acttattcaa taaacggaac taatgcagat
    13141 ttgattgtag acggacgtat tattcagtgt tatgtagatg aaaatcgagc aaaacagcgt
    13201 ctacggattt attatgcaaa aacttctgta ataggcaata cgatagaagt aaaagctgaa
    13261 cctattttca atgatataag aaaatcggtg ttgaataaat atgacagcgg aacagaaaag
    13321 atcactgcta ctcaggcatg gcaaaacgca aaagttttag cgaaaccagc tatcccttcg
    13381 cagttttctt tctcgtcatt agtagatacg cttgctaatg tgaagataga aaaggcgaat
    13441 tttttagaat tctttggtgg aaaagaggga tctattctag atcgatttca tggtgagttt
    13501 ctaaaagata ataacacatt acgtcatgaa aaaagtctag gtacggatca taaaatcaaa
    13561 gcgatttata ctaaaaactt aactggtctt gacttagaga ttgatgctca aagtgtttta
    13621 gttggagttt atccattcat tagcagctct tcagaaggag aagacgagat cactctacca
    13681 gaagaagtta ttttcacgga ttacgtggat gattatcctg ctggatatgt ttcttttgtt
    13741 gattttaaag acaaagcgac tgatgtagcc acattaaggg aagctgctaa agactggttg
    13801 aaaacaaaca tagataaaca aaaaccacaa gtgagtggtt cgattgaatt agtaccattg
    13861 aggcatcaaa gaggctatga aaaatttgtt gatctagaaa aagtttcgat gggtgacgga
    13921 gtagatgtgt atcatccaca gttaaaagtg aatatgtcag cgagaattgt ggaatatacg
    13981 tttaatgttc taaccaattc atacgataaa ttagttgtag gaaacgtcaa aacaaacttc
    14041 ttagaaaaca cagagaataa tgtcagcaat ttgattaatg atgccattga tcaattgaaa
    14101 aacggtggcg aaatcagtga tttactcaat gatattgtag atcatcaaac tgatatgatt
    14161 actggtcaaa atggtggtta tgttttatta gatcctaaag aagcgcctag tcgtattttg
    14221 attatggaca caccagataa gaataccgca aggaatgttt tacaaatcaa caatgctggt
    14281 attggttttt ctaaaactgg cattaatgga acatatgaaa cggcatggac gttagatggc
    14341 ggattcaatg cctcgtttat tacggctggt gaaatagtag gaattactat tagaggtact
    14401 acattaatta gtgatggtgc tgattataga acaagtattg ctaatggcaa aatgacttgg
    14461 tactcaaaaa aagttaacaa agatattatg gagctagaag cacgtgatta tgtaagtgct
    14521 gatgccggta ttgtatcata caccatgaaa actggtggtg gtttcatgat tagaaatcca
    14581 cagggtaact tggtttttag tacgtgggat aatggtaata acagaccgtt tctatctttt
    14641 ggtgcgccca atttcaggta tagcaatgct agttatgtaa cttctggcga cggtagttct
    14701 ttaagcatta atggtagtgc gggtaactca tgggaattta aggtagctgg taggactatg
    14761 aaatttacta gtgatggtat gctaacgtta ccaggttgtt tttttggttc atgggaagat
    14821 gggaaacttg ctaggtttga acaatcaacg gtacaagtat ataaagattt tactgttaga
    14881 ggtactaaaa actcaactgt accaacagaa cattatggac aacgactatt gaacgcttat
    14941 gaaactccag aatattattt cgctgattat ggggaagccg ttacaggtga caatggtaaa
    15001 gttcgtgttg atattgaccc catgtttgct gaaacagtaa atctaagtcg gtatatgaca
    15061 catgtgacac ctacagaact agttttgtgt gctgttactc atgaagatat tgaccatttc
    15121 atcattgaaa ctagtaagcc aaacgtatta gttagatgga atttagtggc acaccgtcta
    15181 gggtatgaag atattagatt aaaagaggat acagcatatg atagcacagt gcttgaccaa
    15241 aaacgttttt aaaacgaaga caaggaggta tataaatggc tagcagttta tataatttgg
    15301 ctttagattt cagcaaagaa ttaaactaca ccaaagctat tatggctcgt caaggtgata
    15361 aagggattac ggtgacggtt aaaccgtttc taaatggctt gcagatggat acgagtggcg
    15421 gaacatttac tttaaaagga acaacaccat ctaaccgtta cgtagataat gttgcaacta
    15481 gtgtaactag tgaagaagtc acgttttctc ttgatggcac atttatgagt gaagcaggat
    15541 attataaaca ctgctacgta gaatatagaa aagacaatca aattttaaca acgcaagata
    15601 tcattttttt ctcactagga gtgtctgaca tttcgcaagg ccaagccgat gaatatgttt
    15661 cgcaattaga agagttgatt cgaaagtaca acgaaacttt tgatgctttt atggctgaaa
    15721 tcaaaggtag agtggatagc ttaaatcaac agattactga tttaactggt caagctaaaa
    15781 cgctacaaga caagttagat gctctgaaag aagaaatttc taagttaggt aacttacaag
    15841 tgatgtacag taacagcatc gacttcgggg actatgatta ttcggagaac ccgaatttaa
    15901 tgccatatat tacagaacct tgggttggac ctttgttagg taatggacat acagttaaag
    15961 atagtgttaa aagggttata actcatacta aaactagaac agctaattcc ggcgacatat
    16021 tatcgcttgg cttaggtatt ccgtgtacag cggaagctaa taataggtat cttataacca
    16081 ctttacgacc atctacgaca tacacattaa gcgttactat gagtgtcggt tcagattgga
    16141 ctggagagac caatactata ggggttaggc ttagatattt aaatgaacaa ggtggtatag
    16201 aactgcccat taatgcatta ataccagcaa atgtagaacg cgataaaatg gtcactcata
    16261 catttactgg tataacaaaa gataatgtaa ctagcataac aaactgttat gttgaaatat
    16321 tctcacttaa tagtgaatat aaaggaacgg ttagtgtcag ctatgacgtt aagttaaaag
    16381 cacactaccc taacttacta gatggccctt actggttagg taaagttcct ttaggcgaga
    16441 atattgctga ccctacagtt gtctttccac acaaaactag tgaatatatg gtttatggta
    16501 gacgcaatac agaaaactat atcgctgacc aaacatatac catatcaatg aaagcaacta
    16561 aactaacagt tcaatcgttt gccgtgtata ttgctgctgg ccgcgttaaa gtaggagaca
    16621 tgaagcctac tgaggggtta gctaatacat gggagttaac ttttactgta actaagcaac
    16681 atatagatag tggggtaact aattatctgg aaatatacca atacccgtca gctacaaaag
    16741 gagctgttca aattgagtgg ctcaaactag aaaaaggcaa tactcgaacc ccgaatatta
    16801 gtgagtataa atatcgtggt actggtatgc gtgattcaaa caatccaaaa gattatgttt
    16861 gggatctagc accagaatat gtcgaagata atttggccac agatattaaa atttctgaaa
    16921 ttactggtaa agcaaacaat tataccgatg ggaaagtatc ggagattaat tcgcagttga
    16981 ctgcttcaat taatgaagta gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta
    17041 atgcgactgc tatagatgaa ttagacaata agatcgatga acgcattaat gatacagcta
    17101 ctaccacatt aacagttaca aacgggaata ccggatcagc aaagctttat cgtgaaggaa
    17161 aaacagtttc tatatatttt gtggctttaa acggaaaacg cagtggtgga aatgattcaa
    17221 cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct
    17281 cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta
    17341 aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt
    17401 agaaaagcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag
    17461 caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg
    17521 attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga
    17581 aatatggacg tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg
    17641 ttccttacac agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa
    17701 tcgctgatca aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag
    17761 atcacaacga tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg
    17821 atttaaaaca gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata
    17881 ctgcattgaa agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt
    17941 cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg
    18001 gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttc
    18061 actaacgaac aagtaatggt tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa
    18121 atcattagca aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag
    18181 ttgtcacatg attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt
    18241 ttctttgatt ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac
    18301 tctttcaaaa gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc
    18361 agatcgattg atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatgc
    18421 agaaaaattg gtagaacaca cgcaacaaat taaaacttta tttagggaga gaagaaaatg
    18481 aataataaaa cgttcgaagt actaaaatgg ttcgcactgg taattattcc cgcactagct
    18541 actttcgtgg ggttagttgg taaagcgctc aattggcagt acacagatat ctgtgttgtc
    18601 atcattactg gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac
    18661 aaaatgttct cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa attactatta
    18721 ctgcgatgag cctgttaacg gctctttttt tattacccat taatacgttt gcttatacta
    18781 ttaatgacga gtataattta gcgccgaatc aaggagactc cagattagca attcctaaca
    18841 aaattatttt gcacgaaact ggaatagatg caccagcaag aaacgtagcc gccaacatga
    18901 aaaataatta taacggaagc aattcttata ctacagatgt tattggtgac ggtgggattg
    18961 tttaccgtgt gggtgagcaa ggatatgttt cgtggggagc tggtaacgct aatccttatg
    19021 cgcctgtaca gattgaatta cagcgcacat atgataaagc attgtttgaa aaaaactatc
    19081 gagcttatat tgaatataca agagatagtg caaaaaaata tggaattcca ttgactcttg
    19141 atcaaggaac ttctttattt acaaaaggaa tcatttctca tttgtgggtg acaaattatg
    19201 tttgggggaa ccacacagat ccatatggtt acttatcg
//
LOCUS       DAHHPA010000053        19197 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.054, whole genome shotgun sequence.
ACCESSION   DAHHPA010000053 DAHHPA010000000
VERSION     DAHHPA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.054"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            19..936
                     /locus_tag="NBH55_001712"
     CDS             19..936
                     /locus_tag="NBH55_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1509698.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3116
                     /locus_tag="NBH55_001713"
     CDS             939..3116
                     /locus_tag="NBH55_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1509699.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3113..4114
                     /locus_tag="NBH55_001714"
     CDS             3113..4114
                     /locus_tag="NBH55_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCD1509700.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4111..5043
                     /locus_tag="NBH55_001715"
     CDS             4111..5043
                     /locus_tag="NBH55_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1509701.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5288..5404
                     /locus_tag="NBH55_001716"
     CDS             5288..5404
                     /locus_tag="NBH55_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCD1509702.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5420..7339
                     /gene="tet(M)"
                     /locus_tag="NBH55_001717"
     CDS             5420..7339
                     /gene="tet(M)"
                     /locus_tag="NBH55_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCD1509703.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7458..7625
                     /locus_tag="NBH55_001718"
     CDS             7458..7625
                     /locus_tag="NBH55_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCD1509704.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7685..8038)
                     /locus_tag="NBH55_001719"
     CDS             complement(7685..8038)
                     /locus_tag="NBH55_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1509705.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            8543..8965
                     /locus_tag="NBH55_001720"
     CDS             8543..8965
                     /locus_tag="NBH55_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD1509706.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            8962..9192
                     /locus_tag="NBH55_001721"
     CDS             8962..9192
                     /locus_tag="NBH55_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1509707.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            9653..9856
                     /locus_tag="NBH55_001722"
     CDS             9653..9856
                     /locus_tag="NBH55_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCD1509708.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            9938..11155
                     /locus_tag="NBH55_001723"
     CDS             9938..11155
                     /locus_tag="NBH55_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1509709.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            11387..11713
                     /locus_tag="NBH55_001724"
     CDS             11387..11713
                     /locus_tag="NBH55_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD1509710.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11781..12494
                     /locus_tag="NBH55_001725"
     CDS             11781..12494
                     /locus_tag="NBH55_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCD1509711.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12804..13655
                     /locus_tag="NBH55_001726"
     CDS             12804..13655
                     /locus_tag="NBH55_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD1509712.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13776..15641
                     /locus_tag="NBH55_001727"
     CDS             13776..15641
                     /locus_tag="NBH55_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1509713.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15689..17098
                     /locus_tag="NBH55_001728"
     CDS             15689..17098
                     /locus_tag="NBH55_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCD1509714.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            17206..17907
                     /locus_tag="NBH55_001729"
     CDS             17206..17907
                     /locus_tag="NBH55_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1509715.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            18066..18545
                     /locus_tag="NBH55_001730"
                     /pseudo
     CDS             18066..18545
                     /locus_tag="NBH55_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         6389 a         3293 c         4071 g         5444 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt cactctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtga ttatgaaaaa tcccaaagat
      121 tctgaatcac tggctattga tattctgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtacgaggg
      241 ttgatgaaag tgattgagga attacgggtt gagaatacgc cactaagtac cagtatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcacatt tattattcag taatggttat
      361 gtggagcagt ctatcagctt agaaaaacaa ctgaacatta tacaggttgc ggacttggta
      421 cttcccgaca aggaaacttc ctttgaggaa tataccacta tggagctttt atccgttgct
      481 atgctgattg tcattagtac ctttgcttta gactttatcc atacagaccg aagcattttc
      541 aagattgtag atttagacga agcatggagc tttttacagg tagcacaagg aaaaacacta
      601 tctatgaagc tggttcgggc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagacg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgcattt
      721 cgttccactg accttaacga gattaaaaag accttagcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atagtaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggcttcc gtggaactgg tcggacggga
     1201 ttggaaaaca ggtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtt
     1261 tgtatgtttc caatgcgaca ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggtaagaata tgcagacctt agctggtgtg agtgcaaacg
     1381 gattttcaac agagggtttc tatgttggat tcctcttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacggga ctgataaaga gagaaaccac aaaggcaatt catgccatta
     1501 tgaattttgt gctggtgttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagtaatgc cagtttatca cttggcacga
     1621 agattgtcat gccccattcc gatagtcaag gcaaggacag cgtggactta atcagagata
     1681 gcctgttttc catacaggtt cagcaaccgt ggctactgct tcaatacaac agttcagaca
     1741 ttgaaagtat cggtattgac cgtgtggaaa gcctgctctc caccagccca gattccaaca
     1801 atggcgaaga cagagaaaaa attgttgcgg aagaaattga agacagaagc aataccaatc
     1861 taaccattac aaagaccatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttca
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtgtgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ttccatgact actctagggg ctgggtctgc cattgttaca ggtaaaaaag
     2401 gacagtcggg ttcggggagt tctgcaagga cacaagcaga tcactcccga ccagacggaa
     2461 aggaaaaatc aacacttgga aaacgtatcg gtcaaaccat cggtacagta gctgatacca
     2521 aagacagaat ggtagacact gctagtggtt tgaaagaaca ggttaaagat ttgccgacca
     2581 atgcaagata tgcagtatat caaggaaaat ccaaagtaaa agagaatgtc cgtgatttaa
     2641 ccagtagtat ttctcaaacc aaagcggaca gagccagtgg acgcaaggaa cagcaggaac
     2701 aaaggcgaaa aaccattgcg aagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac
     2761 agcctgcttc ttctgttcat gaaagaccga ctacaagaca agaacaatat catgatgaac
     2821 agacctcaaa acagtctaat attcagactt catataagga atctcaacaa gccaaacaag
     2881 agcgtccagc agttaagtcc gatttttcaa gtccaaaagt ggaacgccaa ggcaataccg
     2941 ttcaagaaaa aaccgttcaa aagccagcaa cttcaaccac tacagcagat agaacttcac
     3001 aacgtccaat cacaaaagaa cgtccgtcta ctgttcaaag agtaccacta caaaatacaa
     3061 gaagtagacc accaatcaaa accgccacca ttaagaaagt cggtaagaaa ccatgaagtt
     3121 gaaaacttta gtgattggtg gttctggatt attcttgatg gtcttctcac tgcttctgtt
     3181 tgttgccatt ttattttcag atgaacagga cagcggaatt tccaatattc attatggagg
     3241 tgtgaatgtt tccgcagaag tgctggctca taagcctatg gtagaaaaat atgccaaaga
     3301 atatggcgtt gaagaatatg tcaacatact tcttgcgatt atacaggtgg aatcgggcgg
     3361 tactgcggaa gatgttatgc agtcctcgga atccctcggt cttccaccta attcattgag
     3421 tacagaagaa tccattaagc aaggtgtgaa gtatttcagt gaattattag ccagtagcga
     3481 aaggctcagt gtagatttag aatcggttat ccagtcctac aattatggtg gtggtttctt
     3541 agggtatgtg gctaatcgtg gaaataaata tacctttgaa ctggctcaaa gtttctcaaa
     3601 agagtattca ggtggcgaaa aagtgtctta ccccaatccc atagccatac ctatcaatgg
     3661 gggctggcga tacaactatg gcaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt
     3721 cacaacagag tttgatgatg atacggtaca agccatcatg gacgaagcac tgaaatatga
     3781 gggctggcga tacgtttacg gtggagcttc cccgactact tcttttgatt gtagcggact
     3841 gacacaatgg acgtatggaa aagctggaat taacttacca cgaaccgcac aacagcaata
     3901 tgatgtgacc cagcatatcc cactatcgga agcacaagct ggcgatttgg ttttctttca
     3961 ttctacctat aacgctggct cttatattac tcatgttggg atataccttg gcaataaccg
     4021 tatgtttcat gcaggcgacc caatcggtta tgccgactta acaagcccct actggcaaca
     4081 gcatttagtg ggagcaggac gaatcaaaca atgagaaagg aagatttaat gatgaaattt
     4141 agaaaaaatc agaataaaga aaaacagata ccaaaggaaa agaaacctcg tgtctataag
     4201 gtcaatcctc ataaaaaggt tgtgattgcc ttgtgggtac ttttagggct tagtttcagc
     4261 tttgcgatat tcaagcactt tacagctata gatactcata ctattcacga aacaactatc
     4321 atagaaaagg aatacgttga tactcatcat gtagaaaatt ttgtagagaa ctttgcgaaa
     4381 gtctactatt catgggagca atccgataag tccattgata atcgaatgga aagtctaaaa
     4441 ggctatctga cagatgaact tcaagctctc aatgttgata cagtacgcaa agatattcct
     4501 gtatcgtctt ctgtaagagg atttcagata tggacggtag agccaactgg cgacaatgag
     4561 tttaatgtaa cctacagtgt agaccagctc attacagagg gagaaaatac aaagaccgtc
     4621 cactctgctt atatagtgag tgtctatgta gatggttctg gaaatatggt actggttaag
     4681 aatccgacca ttaccaacat acctaagaaa tcaagttata aaccaaaagc cattgaaagt
     4741 gaggggacgg ttgattccat tacaaccaat gaaatcaatg agtttttaac gacgttcttc
     4801 aagctctatc ctacagcgac agccagtgaa ctttcctact atgtgaatga cgggatatta
     4861 aaaccaatcg gaaaagagta catctttcaa gaactggtaa atcctattca caatcgtaag
     4921 gataatcaag tcacggtatc gctgacagtg gagtatatcg accagcagac caaagcaacg
     4981 caggtatctc aatttgattt ggtacttgaa aagaacggga gtaattggaa gattatagaa
     5041 taacaaatat tggtacatta ttacagctat tttgtaatca cgtactctct ttgataaaaa
     5101 attggagatt cctttacaaa tatgctctta cgtgctatta tttaagtatc tatttaaaag
     5161 gagttaataa atatgcggca aggtattatt aaataaactg tcaatttgat agcgggaaca
     5221 aataattgga tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct
     5281 ttatcatgtg attctaaagt atccggagaa tatctgtatg ctttgtatgc ctatggttat
     5341 gcataaaaat cccagtgata agagtattta tcactgggat ttttatgccc ttttgggctt
     5401 ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc
     5461 aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg
     5521 aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat
     5581 tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga
     5641 cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg
     5701 ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca
     5761 tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg
     5821 aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat
     5881 caaacagaag gtagaactgt atcctaatgt gtgtgtgacg aactttaccg aatctgaaca
     5941 atgggatacg gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc
     6001 attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt gttctctgtt
     6061 ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta tagaagttat
     6121 tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg gaaatgtttt
     6181 caaaattgaa tatacaaaaa aaagacaacg tcttgcatat atacgccttt atagtggagt
     6241 actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat
     6301 gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt
     6361 tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc
     6421 acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa
     6481 acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct
     6541 tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt
     6601 acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa
     6661 agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat
     6721 cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt
     6781 gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca
     6841 aaatgcagtt atggaaggga tacgctatgg ttgtgaacaa ggattgtatg gttggaatgt
     6901 gacggactgt aaaatctgtt ttaagtatgg cttatactat agccctgtta gtaccccagc
     6961 agattttcgg atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga
     7021 attgttagag ccatatctta gttttaaaat ttatgcgcca caggaatatc tttcacgagc
     7081 atacaacgat gctcctaaat attgtgcgaa catcgtagac actcaattga aaaataatga
     7141 ggtcattctt agtggagaaa tccctgctcg gtgtattcaa gaatatcgta gtgatttaac
     7201 tttctttaca aatggacgta gtgtttgttt aacagagtta aaagggtacc atgttactac
     7261 cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg atagataaag tacgatatat
     7321 gttcaataaa ataacttagt gtattttatg ttgttatata aatatggttt cttgttaaat
     7381 aagatgaaat attttttaat aaagatttga attaaagtgt aaaggaggag atagttatta
     7441 taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta aagataaggg
     7501 aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa gaaaatttaa
     7561 ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag acgcagagcc
     7621 gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt atggattctt
     7681 ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat tccattatgg
     7741 atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt cgggtgcttt
     7801 ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacatgaagt aacgatacaa
     7861 ggtcataaag aacttgtatg ctggggtgtt gccctttatt ttcaatatta gttaagtacc
     7921 gtgggtcaat ttcaatcaat gctcccactt gttcacgagt taaacctcgt ttcaatcgag
     7981 cttctttaat ggctaaacca aaggctctaa aatcatattt atcttctttt ttacgcatag
     8041 tagaccacct ctatacattt tattgttcct actgaattaa aaacaggtat agaaaaacgt
     8101 gttatatggt ttataggttt atatttaata aaaagcacta ctaaacgcca ataaaaaaaa
     8161 ccgttatatg gtagtgctat ttacgctgtt aaaatattgt atattacttc caaatggcgg
     8221 tttgttggag gtcaacgtcg ccatgaagta catcatatac aataaatttc cttacattgg
     8281 gttcttgtca aaaaaagtcg tctatctgca atagataagt acgtccacca atgtggtttt
     8341 ataaatcata tagatagaat aacagaagca tgtaaacaga gaaataaatc tgtttatatg
     8401 cttttttggc tattcagaac ttttttacaa agtttattta tcagtaatgc aacaaatccc
     8461 cctttcacat tgggactaag agtgaaagga gataaacgag caaggctcac ttcctttcct
     8521 agacagaaag ggggtgagaa acatgaaacc atcttctttt cagaccacaa tagaaaatca
     8581 gtttgactat atctgtaaac gtgctatgga agacgagcga aagaattata tgctttatct
     8641 ttcaaggatt gcaaagcgtg aggtgtcctt ttcggatgtt ggcgattatc ttgttagcca
     8701 gtttgcgaca acagataact attcaactga ctttcagatt tttacactca atgggttatc
     8761 agtaggcgtt gaaaatgatt tgttgagtga agcattacgt gagttgccag acaagaaacg
     8821 tgaaattcta ctgctgtttt actttatgga catgagcgat tcagaaattg cagacctgtt
     8881 gaaattgaac cgttctactg tctatcggca tagaaccagt ggactagcct taattaaaaa
     8941 gtttatggag gaatttgaag aatgaaaaca caatatccta tgattccctt tcctctcatt
     9001 gtaaaggcaa cagatggcga taccgaagcg attaaccaga ttctacatca ttacagaggg
     9061 tacataacga agcgttccct acgacttatg aaagatgaat atggcaatca aagtatggtc
     9121 gttgatgaag tcttacgtgg aagaatggaa accagactga ttacaaagat tttgtcattt
     9181 gaaattaagt aatatcctct ctcctttcgt ggaagcgtgc taaaccattc cacgcttccc
     9241 gaacagggag gtttgttatt ccaccaaagc atattgagct ttcaatgtgt tttgataggc
     9301 taacgagcca ttgttctttg aaaactgaat aaaagtaatc gaatacgttt cgataagaaa
     9361 agagccaacg gaactaaccg ccatgaccta tcttataaag atagcgagcg attcatgtta
     9421 gtgatccgag aagcaatctt tagcaggatt gcctgcaacg acattcttat cgtgataatg
     9481 atactcccat acagtcaata gtccgagcgt gataaaaccg tcgcaggcaa tgagtatggc
     9541 tacatgagaa ccatgcaggg gtggaactcc cgtgagcttt gctaaagctg ttcgattgct
     9601 ggtaaaacaa cttttatgaa atccaaataa gtgatttgga aaggaggatt ttatgaagca
     9661 gactgacatt cctatttggg aacgttatac cctaaccatt gaagaagcgt caaaatattt
     9721 tcgtattggc gaaaacaagc tacgacgctt ggcagaggaa aataaaaatg caaattggct
     9781 gattatgaat ggcaatcgta ttcagattaa acgaaaacaa tttgaaaaaa ttatagatac
     9841 attggacgca atctagcgtc gccaaagggt cttgtatatg ataaaatagt attaagtcgt
     9901 atcaaggctc tttccataaa ggaaaggagc aaatgccatg tcagaaaaaa gacgtgacaa
     9961 tagaggtcga atcttaaaga ctggagagag ccaacgaaaa gacggaagat acttatacaa
    10021 atatatagat tcatttggag aaccgcaatt tgtttactcg tggaaacttg tggctacaga
    10081 ccgagtacca gcaggaaagc gtgattgtat ctcacttaga gagaaaatcg cagagttaca
    10141 gaaagacatt catgatggta ttgatgttgt aggaaagaaa atgacactct gccagcttta
    10201 cgcaaaacag aacgctcaaa gaccaaaggt tagaaaaaac actgaaactg gacgcaaata
    10261 tcttatggat attttgaaga aagacaagtt aggtgtaaga agcattgaca gtattaagcc
    10321 atcagacgct aaagaatggg ctattagaat gagtgaaaat ggttatgctt atcaaaccat
    10381 caataactac aaacgttctt taaaggcttc attctatatt gctatacaag atgattgtgt
    10441 tcggaagaat ccatttgact ttcaactgaa agcagttctt gatgatgata ctgtccctaa
    10501 gaccgtacta acagaagaac aggaagaaaa actgttagcc tttgcaaaag ctgataaaac
    10561 ctacagcaaa aattatgatg aaattctgat actcttaaaa acaggtcttc gtatttcaga
    10621 gtttggtggt ttgacacttc cagatttaga ttttgagaat cgtcttgtca atatagacca
    10681 tcagctattg agagatactg aaattgggta ctacattgaa acaccaaaga ccaaaagtgg
    10741 cgaacgtcaa gttcctatgg ttgaagaagc ctatcaagca tttaagcgag tgttagcgaa
    10801 tcgaaagaat gataagcgtg ttgagattga tggatatagt gatttcctct ttcttaatag
    10861 aaagaactat ccaaaagtgg caagtgatta caacggcatg atgaaaggtc ttgttaagaa
    10921 atacaataag tataacgagg ataaattgcc acacatcact ccacatagtt tgcgacatac
    10981 attctgtacc aactatgcaa atgcaggaat gaatccaaag gcattacagt acattatggg
    11041 acatgctaat atagccatga cgctgaacta ttacgcacat gcaacattcg attctgcaat
    11101 ggcagaaatg aaacgcttga ataaagagaa gcaacaggag cgtcttgttg cttagtagta
    11161 caaatgaatt tactacttat ttaccacttc tgacagctaa gacatgagga aatatgcaaa
    11221 gaaacgtgaa gtatcttcct acagtaaaaa tactcgaaag cacatagaat aaggctttac
    11281 gagcatttaa gaaaatataa aaagataatt agaaatttat actttgtttt atgtaaaaaa
    11341 atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga atagtatgga
    11401 aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg aaaaagattt
    11461 ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat ggaaaacgcc
    11521 ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag atgtattgat
    11581 ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca ttgaagaaaa
    11641 attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac gaaaaaaata
    11701 cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa aacagtaaaa
    11761 tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg ctagtcaata
    11821 tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa cagatcaagg
    11881 attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca aaagaagatc
    11941 tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa ccaatgacga
    12001 cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt gctagtatcg
    12061 cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat acgtcactac
    12121 tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat tatgccttag
    12181 caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat ttagagatgg
    12241 gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca ttttttaaag
    12301 aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa gaaattgtca
    12361 cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa ggagaactgg
    12421 aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc aatctagaga
    12481 caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag ccaaaggata
    12541 tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga ggtgcatctc
    12601 tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt atatgaaaag
    12661 gcttgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg attgttactg
    12721 tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag gtttttttat
    12781 ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac aatgtcgtca
    12841 tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta gcttttcaaa
    12901 aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttgtt ttaagagatt
    12961 cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag gagatcgaat
    13021 gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt tcgcctaata
    13081 tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa gaaaatatcc
    13141 aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa tatcaagcag
    13201 cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct gaggatgaag
    13261 ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac atgcaagtga
    13321 cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa ttcttcggca
    13381 tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg cagttttttg
    13441 cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc tacgcattag
    13501 ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag tatcttcgac
    13561 aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt catattcaaa
    13621 gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat ggcaggcaaa
    13681 acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa tctgggtttt
    13741 tattttttaa ataaaaaaga aagaaggaaa aaacaatgga taatcaagca gttggtcgtc
    13801 gtgtatggga agcggtagga ggacaaaaga atgtcaaaag tttagtacat tgtgctacgc
    13861 ggttgcgatt cagattaaaa gatgaatctt tagcagatac gcaaaagtta aaagaagacc
    13921 cggacgtgat ccaagtcgta caaagtggcg gtcagtatca agtagtgatt ggcagtaatg
    13981 tagcagatgt ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg gaggatggaa
    14041 aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat tatctctagt atatttacac
    14101 cttttctagg ggcgatggca gccgccgggg tattgaaagg attcttatca ttggcaacgg
    14161 tgatgggctg gctgacagct gatagcggta cttatcaaat attgtttgct gcagcagatg
    14221 gtgtctttac cttcttacct gtcatgcttg cttttacagc agctaaaaag ttcaaagcca
    14281 accagttttt agcagtagca attgctatgg cacttgtgta tccagctatc acagcagttg
    14341 ctggtgcggg agaagctatc agtttctttg ggatcccagt gattctttca ccatctggtt
    14401 atacttcttc tgtcatacca atcattctag ccgtatgggt tcaaagtaaa ttagagccat
    14461 tcgtaaaaaa agtgattccg caatttctgc aaatgatttt agttccgctt gtcgttttag
    14521 tagtgatggt tccattaaca tttcttgctt taggaccaat cgggacggtt gccggtaatg
    14581 ctttaggcgg tctcttcaac tcgatttatg gatttagtcc aatcgtagca ggtctaatca
    14641 tgggaagttt atggcaagta ttcgtgatgt tcggcatgca ttggggattc gtaccgatca
    14701 tgtttttgaa tatcgaacaa tatgggtttg atgtattgat gccaatgctt cttccagcca
    14761 tcttggcaca aggtggggct gctttagctg tagcattgcg aacaaaagat acgaaacttc
    14821 gagcattagg gatttcttct acagtgactt cgttatttgg aatcactgaa ccaactgtgt
    14881 atggtgtgac attacctttg aagaaaccat tcattgccgc ttgtatttct ggaggtattg
    14941 gtggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg gtcagcttat
    15001 tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg acagtagcag
    15061 taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt ttaggatttg
    15121 acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga gaaccgatta
    15181 cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca ttgagtgaag
    15241 taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt gatccagaag
    15301 taggagaact agttgcacct gcagacggag agatcacgac tatttttcca actggacatg
    15361 ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg atggatacag
    15421 tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc gtcaaagcag
    15481 gagatttatt gattcgtttt gacatcgaag caattagagc agctggctat agcgtaatca
    15541 ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta gaccaaaaag
    15601 agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat caggatgcaa
    15661 tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc aattagaagg
    15721 ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg gaggtaagca
    15781 gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag aaaaatatat
    15841 ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc ttgccttgtt
    15901 tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa tttttccaaa
    15961 tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc tgattgatga
    16021 gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa tgcctcttca
    16081 tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct atgaacgatt
    16141 tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga ctttcaatga
    16201 aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa gcaatggagc
    16261 aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa gtagcaaagc
    16321 cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga ttatctatgc
    16381 aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga tccaaaacca
    16441 agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg catacacagc
    16501 gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg aaaaagactt
    16561 ccagttgcta aaagaatatc cagtggacta catcggattt agttattata tgtctacggc
    16621 aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg gaggagtaaa
    16681 aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag ggctgagaat
    16741 cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg aaaacggtct
    16801 tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc ggatcgatta
    16861 tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag atttgatggg
    16921 ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa tgagtaaacg
    16981 ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga atcgatcaaa
    17041 gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa acttgtaatt
    17101 aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct ttttttattt
    17161 tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa aaggacacgt
    17221 gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg aaaatatcca
    17281 gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt cagcaaaatc
    17341 actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa gaaaagtggt
    17401 cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc ggcaagcttt
    17461 acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga tttgcaattg
    17521 gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc tgtttgtttt
    17581 aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta tcttccacaa
    17641 gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg tttgctgacg
    17701 aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt tttaacagaa
    17761 gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat acggaaatcg
    17821 tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac ggagctgcat
    17881 ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa gctgccctgc
    17941 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttaggct
    18001 tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa gggggtgtgg
    18061 cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct tcgatggtaa
    18121 ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa aatatactca
    18181 atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt cagatcacag
    18241 agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca acgggagtcg
    18301 gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt attccggcat
    18361 cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt gcattgttag
    18421 ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta gatccgaatg
    18481 cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat ttgacaatta
    18541 gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct tgagacagga
    18601 atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt ttaggagcga
    18661 tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa aaatgagaac
    18721 ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac aaaaaaactt
    18781 tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga aattattctt
    18841 catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca ggcaggcgca
    18901 acaggtgcgg ttatcactga attttcttcc agcagtgacg attcaagtga tatttttaca
    18961 aatccagcta tttatcgtta acaaaaagat ttttaggaga agctgatgaa caaaaatgat
    19021 caaattttga tcgtttagtt cgtcggcttt tttatttaga gaaacatcat ttatcagtga
    19081 aacataacac gagaacaaaa tgattttttc gttaataaaa gaaacaagta tttgttgttt
    19141 aaaataatac acgttattgg catataggac gaaatcccag tatcttctat aagtaag
//
LOCUS       DAHHPA010000054        19005 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.055, whole genome shotgun sequence.
ACCESSION   DAHHPA010000054 DAHHPA010000000
VERSION     DAHHPA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.055"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(142..306)
                     /locus_tag="NBH55_001731"
     CDS             complement(142..306)
                     /locus_tag="NBH55_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509716.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            601..1929
                     /locus_tag="NBH55_001732"
     CDS             601..1929
                     /locus_tag="NBH55_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCD1509717.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2131..2964
                     /locus_tag="NBH55_001733"
     CDS             2131..2964
                     /locus_tag="NBH55_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCD1509718.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2980..3717
                     /locus_tag="NBH55_001734"
     CDS             2980..3717
                     /locus_tag="NBH55_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCD1509719.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3872..4840)
                     /locus_tag="NBH55_001735"
     CDS             complement(3872..4840)
                     /locus_tag="NBH55_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCD1509720.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5072..5905
                     /locus_tag="NBH55_001736"
     CDS             5072..5905
                     /locus_tag="NBH55_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCD1509721.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5939..6778
                     /locus_tag="NBH55_001737"
     CDS             5939..6778
                     /locus_tag="NBH55_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1509722.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6804..7364
                     /locus_tag="NBH55_001738"
     CDS             6804..7364
                     /locus_tag="NBH55_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1509723.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7563..9284
                     /locus_tag="NBH55_001739"
     CDS             7563..9284
                     /locus_tag="NBH55_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCD1509724.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9449..10591
                     /locus_tag="NBH55_001740"
     CDS             9449..10591
                     /locus_tag="NBH55_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1509725.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10607..11818
                     /gene="thiI"
                     /locus_tag="NBH55_001741"
     CDS             10607..11818
                     /gene="thiI"
                     /locus_tag="NBH55_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCD1509726.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12391..13038
                     /locus_tag="NBH55_001742"
     CDS             12391..13038
                     /locus_tag="NBH55_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCD1509727.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13167..13383
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13431..16076
                     /locus_tag="NBH55_001743"
     CDS             13431..16076
                     /locus_tag="NBH55_001743"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCD1509728.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16386..17699
                     /locus_tag="NBH55_001744"
     CDS             16386..17699
                     /locus_tag="NBH55_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCD1509729.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17689..18342
                     /locus_tag="NBH55_001745"
     CDS             17689..18342
                     /locus_tag="NBH55_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCD1509730.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18397..>19005
                     /gene="radC"
                     /locus_tag="NBH55_001746"
     CDS             18397..>19005
                     /gene="radC"
                     /locus_tag="NBH55_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCD1509731.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNT"
BASE COUNT         6400 a         3210 c         4014 g         5381 t
ORIGIN      
        1 ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt ctttttcttt
       61 tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc tttacgaaag
      121 agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata cgaaataaat
      181 cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga cattcccaaa
      241 gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag caatcaggtt
      301 tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa tgagtatttt
      361 gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac aggccatttt
      421 cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt atgacatttt
      481 tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata gaagagattg
      541 tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa ggatgattgg
      601 atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca agcaaatatc
      661 aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc tgctgaaaat
      721 gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac ggggaaagtg
      781 gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac attccgtcat
      841 aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta cacttttttc
      901 tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac aggaaaagag
      961 gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga tggcggccaa
     1021 tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc ggtgatgccg
     1081 gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa attactgcgt
     1141 aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga cgctattgaa
     1201 gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc attaggaaca
     1261 cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca tcttgaccga
     1321 ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga tgattatgtt
     1381 agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac agtagagatg
     1441 ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat ggaaactttc
     1501 aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg ttgtgatgta
     1561 ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc aatggatgat
     1621 ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg agaaagtctg
     1681 atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc aacaaaatgg
     1741 aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt tatgagtgat
     1801 gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt gacgaaagag
     1861 caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc aatgggtgct
     1921 ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga gctggcttaa
     1981 gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg tgctataatt
     2041 ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg atgacgaagt
     2101 agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt atctgatctg
     2161 aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac ctatcgtata
     2221 aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa aggatttttt
     2281 gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc ggatacgtat
     2341 atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga gccgggaaaa
     2401 ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac agcgccacaa
     2461 tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc ttttgaagaa
     2521 gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt ggcagaagaa
     2581 acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga acgtgtattg
     2641 aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct gtggagtggt
     2701 aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt ttcttatggg
     2761 aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc ggaattttta
     2821 gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact gccgatttac
     2881 cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg ttcccaagta
     2941 gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa atataccaaa
     3001 aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt tccttcgatg
     3061 ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag aggcagtgaa
     3121 ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga gattaatcgt
     3181 ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta caataagttt
     3241 ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt caaaatagaa
     3301 tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa tgaagagatc
     3361 attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa aattgaaaag
     3421 attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga tagtaatcag
     3481 catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata tgactttttg
     3541 actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga gtacgggaat
     3601 gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac aaaacatgaa
     3661 atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa taaataaaaa
     3721 agaggttgtg acagaagggg gcaacttcaa gaaataagaa gaaatttacg aaaattgttt
     3781 ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag ctatttttgt cacagcctct
     3841 ctttttaagt ttagctatta gataatgatg tttagttttg gataaacgaa atcaattctt
     3901 cacccgacaa accatagttt acagcaatca atagttttaa acgtgctttt tgactattaa
     3961 tactgttaca gaaaataatc cccatttttt ttagttcttt tcccccgcct tcataatcat
     4021 aaacaggctc tgcgattcca ttgaaacaac gagaaacaag tactacaggg attttttgga
     4081 caagaagctt ttccaaagca gctaaggctt gtggagggag attccccgca cctaaagctt
     4141 cgattaccaa accgtctact ttcgtatggg cgatggcttc caataagtcc ccttgcatcc
     4201 cagcatatgc tttaacgatc gggatagtcc catctacagc ggaaatgtcc agtcgctttg
     4261 tttctaaaag ttcctgcaag aataaaattc gattcttagt tactaagccg atgggaccca
     4321 atgtaggtgt tcggaaagta gcaacatttg tggtatgggt ctttgtcacg tagcgtgcag
     4381 aatggatctc atcgttcatg accaccaaaa cccccttatc tagcgcttct tcacaacttg
     4441 cgacacgaat cgcactttca aaattataaa ggccgtcact tccaagttca ttacttgaac
     4501 gcattgcccc agtcaaaaca attggtaatt tgttgccaat cgttgtatca agaaaaaaag
     4561 cagtttcttc taatgtatct gtaccgtgtg tgatcaccac tccggatata cctgatgcat
     4621 aagcggacaa gatacgttct tttaatacca acatgtgaga aggcatgatg tgtggtgagg
     4681 gaagattgaa gatatcttca actaataatt ctacatgctt ggggattgtg atttctgcat
     4741 ctaataatgg gtttgccaca tcaggactaa caccgcccgt gagcatgttt tctttcatgg
     4801 cgatcgttcc gccggtgtgt agtacaagta tttttttcat tttacagact tccttacgaa
     4861 taattttgca tagccgttaa atcttattgt atactatata aaagagtttt gctatcagtg
     4921 aagaataacg aatagcctaa cttatcataa aaaagctgac cttattaaaa tggaaaagac
     4981 tcaaccttga cctcctaata caatggagta tctggttgag tttcttttac aagaaagcag
     5041 ccaaggaaat caatcacgaa ggtggccgga aatgatcaga atcggattaa catcgtttaa
     5101 agaacatgac aaattaactg gtaaaaagaa taacactctt tatgaatatg cagggtttct
     5161 acccttagtg gaattagata ctgcctatta tggaattccc cgaaagtcct cagttgagaa
     5221 ttggataagt gaagtacctg aatcatttcg ctttgtagtc aaagtctatc aaggaatcag
     5281 cggtcaagga gactggaagc cttattatca gaccgaagaa gagatgattg cgcatttttt
     5341 agatgttatg tcgccaatgg tagatgctgg aaagatgttt tgctttttag tccaattccc
     5401 ctcatcattc aaatgtacga aagaaaatgt ccagtacttg cgcaaactta gaaagtggtt
     5461 tgagggttat cctgtttcta tagaattgcg ggattattct tggtacagcg atgaatttct
     5521 tgaacgtaca agaaacttta tgtctagagc cgctttttct ttagccatca tcgatgagcc
     5581 acagttgctg aatacaacga ttccttttga tccatttgtc accaatccga actttgctct
     5641 ttttcgattc catggtagga atgcgcaagg atggcaagca aatgataaag agtggcgtaa
     5701 aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa gaactagcac atgaagtcaa
     5761 agtagttgat tctaagacaa aagaggtagg tgtgattttt aataataatt ctggtggtga
     5821 tgcggctgaa aatgcattag cactgcaaaa aatattaaat atcaagccag atggactcaa
     5881 tccaacccaa atggatctgt tttaagggct tattttattc aagaaagggg cgaagtctat
     5941 gatcaaagct attttttttg atattgacgg aacattggtc accaatcgtt caaaagcgtt
     6001 ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac ggcattttat gcggtgtagc
     6061 aacggggcgc agcccagtaa agatcaaaga aatcattgat gaattggaat tagatatgta
     6121 tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc acgattatcg accacccttt
     6181 tgaacaaaaa gtattggaac atattgttga atttgcagat gagaaccatc gacaaatcgt
     6241 ttttggtgcg agaaatcggt tagatggatc aacgacgatg ctattaggac aatcgatttt
     6301 tatcaagcga ctagtcagct ttttaccgcg taaattcccg gtacgtctga tgaaaaaaat
     6361 cctccaggtt tttagccctc accgacaaaa ggatcgttat gaaacgttag agatattaaa
     6421 agaaccaatt tatcaatgta tattgctcag tccggagtca gaacagaaaa agttagaaca
     6481 tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat actgtagata tcatcccaaa
     6541 aggtggttca aaattactag gcatccaagc tttcgcaaat gctgtcgaaa ttgaaatgga
     6601 agaaattatg gcattcggcg atcattacaa tgatatcgag atgctgaaag gtgtaggaat
     6661 tggagtggcc atggggaatg ctcagatcga agtaaaacat gctgcagact ttgtgactca
     6721 atcaaatgaa gaagatggga tatacgaagg actgaaacac ttcggtctga tttattagtc
     6781 aatgaaagaa ggaaaaaatc agtatggaag cagagacacc ttttcaaaaa gtagaaacat
     6841 tccacaaaac ttttgatcca agaagaccag agatacccac ggcttttaca gaaaatgaag
     6901 ctcatacacg tgctgcgttc aaaatagaag aacttgttga atttgttgca gctacttcac
     6961 ctaataaaga acaactgcat gaagctgtta tgctgctgca cgaagcatta gataaagcag
     7021 aagaaaaaat acttggtaaa acacagtggg gaagtgatcc gctggttgaa caagtagatg
     7081 cactgacaga tatcctttac tttacttatg gttctttttc attactagga atagtgcctg
     7141 atcgaatatt cgaaatcgtc catcaggcaa atatggggaa attatttcca gatggaaaac
     7201 ctcattatga ccctgtaaca aataaagtgt tgaaacctga aaactggcag atggaatatg
     7261 ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat tcaagaagcg attgctaaga
     7321 agaaatccgc tgatataggc atgtgcacaa atgaaaaaga gtgatcagag tttcactatt
     7381 tttgttatct ttatacgatg gaaaaacaaa agattttcgt ttaagttagt gaatgcatga
     7441 aaatagtatg ctataatcta gaatagatgt cttttgcacg agagacgtat tatttggatt
     7501 tatgccaagc agaacgccat ctgttgcata aaagatgcat actgaaaacg gaggaaccat
     7561 aaatgggaaa taatgttatc atcggaatcg tcattgcaat cattatagtc gccattgtcg
     7621 tctttttggt tagttacttg atgaagaaaa aaaaccaaga tcgattggat gccttagaaa
     7681 aacgcaagga agaattattt gacttgccag tgattgaaga agtcgatgat gttaaaaaaa
     7741 tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg gagtcaaaaa tggaatgaca
     7801 tatctacttc ctcgttcgct gaactagaaa gtcaaatttt cgaagtggaa aacctcaatg
     7861 aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga agcagaagct acaatggacc
     7921 aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa ggaattgcgg gaaacggaag
     7981 aacgcaactc actagctgtt cagcaggcac ttgacgtcta tgaagaactg aaagaaaaag
     8041 tcagagaaga cgagcatgaa tacggaccag ctctttttga attgcaaaaa cagatcaaag
     8101 tgatcgaaaa agaatttacg cagtttgtga ctttaaacac ttctggagac cctgttgaag
     8161 cccgcgaggt tttggataaa gcagaaaaac atacgtatga agtggaagct ctgatgaaaa
     8221 aaattccacc gctgtatgag gagctccata ccactttccc tgaacaactt gaagaaatca
     8281 gtgatactta cgagaaattg atggaagaac aatatgtatt cccagaagaa gatcttgctg
     8341 aagatatcgc aaaagtaagt cgaagaattg aaaattcatt agccaatctt gaaaaaacag
     8401 aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga tttgattgat tcgttatatg
     8461 atatcttaga acgggaaatg gaagctcaga gatatgtaaa aactaaccaa tcaacgattg
     8521 cagagtatat caaacataca acaaagaata accgtcaatt attgattgag ctggatcaca
     8581 catctcaaag ctatacattg aaccacaatg aaattggccg tgtacgaggt ttccaaaccg
     8641 aagtggaaga aatggaacgt caaaatgaac agatgattcc acagatccgt caacacgaaa
     8701 ttccttattc agaagtgcgc acgttctata aaacagtctt caaagtctta gaagatatcg
     8761 aaaagcaaca agtagagatc gacgactcat tacatgaatt gagaaaaggc gaaaaagaag
     8821 cgcaagaaaa gatcgataca ttcgaattca aattgcgcag tttgaaacgt tttgtagaaa
     8881 aacaacgttt gcctggcttg ccgaatgatt acctagaatt cttttttgtt gctactgacc
     8941 gtattgaaga actaagtgtt gtcttgaata aaattcgagt aaacatggaa gaggtcaatc
     9001 gtttagttgc tctatgtgaa gaagatctag aattattaga taagaagaca catgatttag
     9061 tagatgcagc agcattaacg gagcaaatgc tccaatatgc aaatcgttat cgtcatacac
     9121 acgaagaagt gagagaagca attgataaaa gctactattt attcaataaa gaatatcact
     9181 atcaagaagc attagacgaa atcggtacag cattagagcg agtagaacct ggtgctttca
     9241 aacgcatcga agatttttat tttaatcatc cagacttagt ataaagcaaa aaagttagta
     9301 aaccagcaag agcaagacgg attattgtct tgttcttgtc tttttttgta tatttgaaaa
     9361 ttgaaacaag tgaaaatttc acctgctctt gaaagcacct tggacaaccg ttataataga
     9421 aaagaatttc aaaaaagagg ggtttcttat gatatatttt gataacagtg caacaacccc
     9481 aatctttccg caaagcttag atgcatatgt aaaaaccagt cagcggatta ttgggaatcc
     9541 atcaagcttg catgatttag gcaatcaagc taaccggttg ttgcagcaag caagaaagca
     9601 gatcgctgat ttgatccatg tagaaccaga agaaatctat tttacttcag gcggaacaga
     9661 aggagataac tgggtattaa aaggaacgat gatcgaaaaa agagaatatg ggaatcatat
     9721 gatcatttca ggagtcgaac atccggccgt cagcgagaca gcagaacaat taaaagaatt
     9781 aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga tttgtacgtg tcgacgaatt
     9841 gaaagaatta ataagaaaag agacagtatt agtctctgtt atggctgtga ataatgaagt
     9901 aggatcgatt cagcccatcc aggaaatcag tgatcttttg gatgattttc ctaaaattca
     9961 tttccatgta gatgctgttc aggcgattgg aaaagtacca ttctcgcaat ggttgacaga
    10021 aagagttgat tttgcaacat tttcagctca taaattccat ggcccacggg gaactggttt
    10081 catttattgg aaaaagggtc gtcgtttagc accattactg acaggtggag ggcaagaaag
    10141 aaatcagcga agtgggacag aaaatgttcc aggtattgtg gctatggtta aagctttacg
    10201 tttatcatta gagataaagg aacaaaaacc tgagcagaca caacgcttaa aagtagctct
    10261 aatcaatgcg ttgaagacgt atgagaaagt gacgattttt tcagaaggaa ctgactatgc
    10321 accgcatatt ttatgttttg ctctaaaagg aattcgcggc gaagttctcg tccatgcctt
    10381 agaagaaaaa caaatatttg tttctactac tagtgcctgc tcctcacgta aacatatggc
    10441 aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg gctacatctg ctgttcgaat
    10501 cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa tttatgatta ttttcaagca
    10561 gctttataag aaattttctg caatcaatta aaagaggagt gttcttgtga aatacagtga
    10621 aattatggtc cgttatgggg aactttcgac aaaaggaaag aatcgaaaaa gctttatcat
    10681 gcaactagca caaaatgtca gacaagcttt gaaagacttt ccagaaatca aaatccatgc
    10741 tgatcgggac cggatgcatc ttttattgaa tggcgcagac gggcagctag ttattccaaa
    10801 actagcaaaa atatttggga tccaaaattt ctctccaagt attcgtgtag aaaaagatgt
    10861 cgaagttttg aaacaagctg ttcaagacat catgaaagaa atttatactg ggaaagaaac
    10921 atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa ttgacaagta atgaattgaa
    10981 tcaaacatta ggtaatgctg tttttgatat tttcccgcat atccaagttc aaatgcgtca
    11041 accagatatt ccattacgtg tagagattcg gcgtgatggc gcttatctat cgtatgaaac
    11101 gatcaaggga gctggaggat tgcccgtagg aacaagcgga aaaggaatgc ttatgctttc
    11161 tggggggatt gattctcctg tagcagggta ctttgccatg aagcggggag tggaaattga
    11221 agctgttcat tttgctagtc cgccgtatac aagtgaacaa gcactgcaaa aagcaaaaga
    11281 tctaacagcc aaactagcac cttatgtggg aacgatccat tttatcgaag tgccatttac
    11341 cgagattcaa gaagaaatca aaaagtcagt gccacaagga tattggatga caattactcg
    11401 tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt cgtcatggtt tagtcatttt
    11461 gaatggtgaa tcattaggtc aagtggcttc acaaactttg caaagtatgg tggctatcaa
    11521 tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca atggataaac ttgaaatcat
    11581 tgaagtagca gaacagatcg atacatttaa tctttctatc cagccttttg aagattgctg
    11641 tacgattttt gctcctccac agccaaaaac acgccctcaa ctggaaaagg tccatcaata
    11701 tgaagagcgt ttagcaatag atgaaatgat ccaacgagca ctagctggct tgaagatgga
    11761 agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa ttttcagaat tgctataaga
    11821 ggttatgaaa atagtgtttt gaccagagta ttagagaaac aaaacgaaaa aatagctcca
    11881 tgtattttac cgtttttgaa ctaatgtcga agattagctg attgaatacc atttatcaag
    11941 aagttatgga aaagccggtt cttttttact aactgaaaaa taaaatttta tttagagatc
    12001 aagctacagt tgataaatga tataaaaaag agaatttccc atgaaatagc gaggttgtga
    12061 cagaagtggg cagcttcaag aaataagaag gaatttacga aaattgcttt tcaaattttt
    12121 gtgaaatttc ggcttatttt tcgaagaagt tacttctgga acaccgtgta tcagaggttg
    12181 cgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt
    12241 ttacgtttgg ttgagcttat taccgcagat gcaagctttt gaacaccgtt ttatttgtct
    12301 cacacccttt tccttcctac ttgtaacctt gctcccgtgt tagaaacatg ttacactaaa
    12361 tatgtgaaat tattaacaag gagcgtcgtt atgcgagaaa aaacagatac actgccaaaa
    12421 gcaactgcga aaagattacc tttatattta cgctacttga agatgctgga tgactcaggt
    12481 ataagtagaa tcaaatcaaa tgagttcagc gaaatcaccc aaattccttc tgcaacgatc
    12541 cgcagagact tctcgcaatt aggcgagcta ggcagaagcg gatatggcta tgatgtccct
    12601 tttcttatcg atgtattcaa taatatcctt aatactaaag aagaaaaacg aatcgcatta
    12661 gtaggatatg gaaatcttgg caaagcattg aaacacaaca attttagaag aaatgaaaat
    12721 ctaaatatcg tgtgcgtctt tgataatgat ccagcactga tcaatcgagt aattgatgga
    12781 gaaatgatct atccgattga tcgtttcgcg gagatagcca aagcaaaaaa tgtgacagta
    12841 gcaatctcta cggtaccaag taaatattca caatctgcta ttgatgaaat tgtcaaagga
    12901 aatgtaaccg ctatacttaa ttttgcgcct gacagagtaa ctgtcccagc ttatgtcaat
    12961 gttcaataca ttgatttgac aaccgagttg cagacgctta tttactttga tgagaattac
    13021 tcagaagttt tttcttagga aaatttgagt aggcttattg atcttgcgag aactcgtcgt
    13081 aattctttca aaaatttttt agtactaaat gaagtggttt tttcacgacc acttgttgac
    13141 taaaaaaaaa aaccattgta gaatgaagca taacgctgaa caagaggagt aactggcaca
    13201 ttcattctta gagagagaac tatctgctga aaggttctca atgacaacca gtgaaggtag
    13261 cttgggagtt tctgtcttga atggagtaga gatagacgag tcgtgatcgt taactcactt
    13321 gagaggcaat aatgattgat tattgcaatt taggtggtac cgcgtaaatc atacgtccta
    13381 gaatacgaaa gtattttagg acgtttttta gtttaaagga ggaaaacagc atgacggaag
    13441 aaaataacct atcaacaaaa tataatccgc aagaagttga agctggacgt tatcagcatt
    13501 ggttagaaca agatttgttt aaaccgagcg gagataaaaa agcaaaacct tattcaatcg
    13561 taatcccgcc cccaaatgtt actggaaaac ttcatttagg gcatgcttgg gatacgacct
    13621 tgcaagatat gctgattcgt caaaagagaa tgcaaggata tgatacttta tggctgcctg
    13681 gtatggacca tgcaggaatc gcgacacaag caaaagtaga agcgaagctt agagagcaag
    13741 ggatttctcg ttatgatcta ggtcgtgaaa agttcatcga tcaagtgtgg gaatggaaag
    13801 aagagtatgc gagccacatc cgcgaacagt gggctaaaat gggcttatct ttagattaca
    13861 gtagggaacg ttttacatta gatgatggat tatctgaagc agtacgtaaa gtttttgtta
    13921 ccttatacga aaaaggtttg atttatcgtg gtgaatatat catcaactgg gatcctcaag
    13981 cacgaacagc tctatctgat atcgaagtta ttcataaaga tatcgaaggc gccttttatc
    14041 atatgagcta tgaactagca gatggatcag gcgtagtaga aattgcgaca acccgtccag
    14101 aaacgatgtt gggagatacg gcgattgctg ttcatccaga agacgagcgt tatcaagcgc
    14161 tcatcggcaa aaaagtgatt ctacctttag tcaataaaca aatcccaatc attgcagatg
    14221 aatatgtaga tatggaattc gggacaggtg tagtaaaaat cactccagca catgatccaa
    14281 acgactttga ggtaggtaat cgtcatgatc ttccacgcgt aaatgttatg gatgatgatg
    14341 gtacgatgaa tgaactagca ggaaaatatg ctggaatgga acgttttgct gctcgtaaag
    14401 caatcgtgca agatctgaaa gaaatcggtc gattgattaa aatcgatacg atgatccaca
    14461 gtgtgggtca ttccgagcgg acaggtgtcg ttgttgaacc tcggctgtct actcaatggt
    14521 ttgtaaaaat ggcgccgtta gcgaaaaaag ccattgataa ccaagaaaca gatcaagcag
    14581 tggaattctt ccctccaaga ttcaatcaaa cgtttttacg ttggatggaa aatgtccatg
    14641 attgggtaat ctcacgacaa ttatggtggg gacatcaaat tcctgcatgg tatcacaaag
    14701 aaacaggtga aatgtatgta gggatggaag caccagcgga tagtgaaaac tgggtacaag
    14761 atcctgatgt tttagacact tggtttagtt cagctttgtg gcctttctca acaatgggct
    14821 ggccagatac ggaaagcgaa gattataagc gatacttccc tactagcact ttagtaaccg
    14881 gttatgacat cattttcttc tgggtaagca gaatgatttt ccaaagcttg gaatttactg
    14941 gcgaacgtcc atttgagaat gtcttgattc atggattgat tcgtgacgaa caagggcgga
    15001 agatgagtaa atcattaggt aatggaatcg atccgatgga agtaatcgaa aagtatggag
    15061 cagatgctct ccgctggttc ttatcgaatg gttctgcacc aggacaagat gtccgcttca
    15121 gctatgaaaa aatggatgct tcttggaact ttatcaacaa aatctggaat gcttcacgtt
    15181 tcgttatcat gaatatcgaa ggaatgaccg tagaagagat cgatttatct ggagaaaaat
    15241 cagtggctga ccggtggatc cttactcgtt tgaatgaaac gatcgaacga gtaactgagc
    15301 tatttgatcg ttttgaattt ggtgaagcag gtcgacaact gtataacttt atctgggatg
    15361 atttttgtga ctggtacatc gaaatgagta aagaagtact ttatggagat gatgctgttt
    15421 caaaacaaac cacccgtagc atcttagtcc atacacttga tcagatccta cgtttattgc
    15481 atccaatcat gccattcgtt acagaagaaa tatgggaaca tattcctcat cagggaaatt
    15541 ctctagtagt ggcagaatat cctgttgtac gtccagagtt tgatgatgaa acagcttcta
    15601 aagggatgga agtattgaaa gaattgattc gagccgttcg aaatattcgt tcagaagtga
    15661 atacaccatt gtctaaacca attacgttgc taattaaaac aaacgatgct aaaatcgatc
    15721 aatttttagt tgaaaataca agctatatcg aacgcttctg taatcccgaa gagttgacga
    15781 tatcaagtga gatcactgcg ccagatctag cgatgtcagc tgttctgaca ggtgcagaaa
    15841 tcttcttgcc gttagcagga ttgatcaata ttgaagaaga aatcaaacga ttagaaaaag
    15901 aactggaaaa gtggacaagc gaagtaaaac gtgtccaagg aaaactttct aatgaacgtt
    15961 ttgtttctaa cgcgccggaa gaagtagtag aggcagagcg tgcaaaagaa aaagattatt
    16021 tagagaagca aacggctgtc aaagaacgaa tcgaaagttt gcgtactatt caataagaat
    16081 agaaaaagag agtgtgaaat cagctagcta ttactaaaag ctagctgatt gaacatcgtt
    16141 acaggaaaga gtttgagcct cactcttagc ttcgattcat aaggagatgt gacataaatt
    16201 ttgtcgcatc tcttttttag tctactctta ttatgtagga gaaggaggga ggtggatcat
    16261 gtatcaagag tcaataaagt agttgataaa atcgttcctc tctctgtttc ttttctcttt
    16321 gtcatacttt tatttttctt atgtgttcta tttgttataa taaaagagaa atgggggaat
    16381 tgttgatgac gattgaagaa gcaatagaat ggatccatag tcgattacca tttggttcac
    16441 gtcctggcct tgaccgagtc gaagctttat tagaagtggt agggcatcca gagagaaaag
    16501 taaaaacgat ccatatcgct ggaacaaacg gaaaaggttc tactgtagct tatttgagaa
    16561 gcctattaga agaggctggg ctgacagtag gtacatttac ttcaccttat atcgaaacgt
    16621 tcaatgaacg gatcgcaatc aatggcaaaa gcattccaga caatgatttg atccagcttg
    16681 taaaaaaata tcagccactg atcaaacaac ttgatcaatt tgaagcagta gctggtatta
    16741 ctgaattcga gacattaaca gcaatggcat ttgattattt tttgatgaag aaagtggacg
    16801 tagcgattat cgaagttggc ttaggcggct tattggattc taccaatgta gctgtaccaa
    16861 tgctgacggg aataacaacg attggacttg atcataccga aatacttgga gatacaattg
    16921 aagaaatcgc tgcacaaaaa gctggaatca tcaaacaagg cgttccagta gtaacaggaa
    16981 atattgcccc agaagctttt actgtcatcc aacaaacagc taaagcaaaa aatgcaccgc
    17041 tttatgtata cggggaaaat tataaaataa actatctgca tcctgatgag atgtggggag
    17101 aagtatttga attttttaat gaaataggaa agatccctca attaaccact ccgcttttag
    17161 ggcaacatca agctgaaaat gcaggagtag ccatcgaatt gtattttgtt tactgtcaat
    17221 tggaaaagct gcctttccga gaaaaagatg ttttgaaggg attgaagaaa tctcgatggc
    17281 caggaagaat ggagcgtttg agtaacgagc cattagttgt tttagatggt gcccataata
    17341 cgcatgcaat gaaccggctt gttgagaatt tgaaaaaaga attcaaagga tatcaaatca
    17401 acattttgtt ttccgcctta cgaactaaaa atgttagaga aatgctagaa cgattgcagc
    17461 aagtaccaaa tgtccatatt tatttgacga cctttgaaca tccaaaagcc atcgaattaa
    17521 gtgaagtatc agatttagca agtgagagag tatcaatcgt ttcattgtgg caattcggat
    17581 tagcagatat attggaaaag atgacaaatg aagacgtatt agttgtgacc ggttcacttt
    17641 atttcatttc agaagttcgc catttattaa tacaattagg aggagatcat gaagtataat
    17701 gcagtgattt ttgacatgga tggtttgctg ttcgacacag agttagtgta ttacgaagcc
    17761 acacaagtag tagcagacaa gatgggtttt ccttatgata aagacctgta tttaaaattc
    17821 ttaggtgttt cagatgaaga agtatgggcg aattatcata tgattttctc ggaatttggc
    17881 aaaacaagtg tacaaagatt catagatgat tcataccagg aaacgcttga acgtttttcg
    17941 caaggagcgg tacaaatgaa gcccggtgtg ttggaactcc tctctttttt agaagaacat
    18001 cagattccca aagttgtcgc ttccagtaac caacgagcag tcattgaact tttattgaag
    18061 aagaacaatc tgtttgaacg atttgagacg attgtttctg cagaaaatgt tcaacgagca
    18121 aaaccagacc cagaaatttt cttgttagct aatcagaagc taggtacagc gaagaaagaa
    18181 acattgattt tagaagattc acaaaatggt attttagcag cttctgcggc tgagatcccg
    18241 gtagtcatgg taccagatct gcttccacca tcccaagaat tgatcaaaaa aacagctggg
    18301 gttgtttctt ctttacatga aatccctgca ttgttaaaat aaaaatccat ctcttttcgt
    18361 atcttatttt agataagata gaaaggagat gtttttttgg gaaaacgatt gatcaaagaa
    18421 gtacctacta gctctttacc aagggaacgt atggaaattt atggcgcgga agctttatcg
    18481 gatcaggagc ttttggcaat cttactgcgt accggtcaac acccctatag tgtgatgtcg
    18541 attgctggaa atctgttaaa aacgtttgga ggactggcct ctttacgaca ggctactttg
    18601 catgagttag aagagattcg agggatagga cgcgtgaaag caatcgaaat caaagccttg
    18661 atcgaattag gtcgacgaat tcagacggat catgaaagag cggctccacc ggttcgtacc
    18721 agctacgaat tggcccagca attgattctg gaaatgaaag atcacaaaca agaacatttg
    18781 gtttgtattt atttggacac gaagaatcaa gtgatcttaa aaaaaacagt gttcatcggt
    18841 tcgctgaatc agtcagtcgc tcatcctaga gaaattttcc attatgctgt acgttattgt
    18901 gcagcaagga tcgttttagc gcataatcat ccttccggaa tatataagtt agtacacata
    18961 aattatataa ggaaatttac tgttatattg ccatctggaa atact
//
LOCUS       DAHHPA010000055        18888 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.056, whole genome shotgun sequence.
ACCESSION   DAHHPA010000055 DAHHPA010000000
VERSION     DAHHPA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.056"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..435
                     /locus_tag="NBH55_001747"
     CDS             <1..435
                     /locus_tag="NBH55_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCD1509732.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="NBH55_001748"
     tRNA            complement(759..842)
                     /locus_tag="NBH55_001748"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="NBH55_001749"
     CDS             1370..1870
                     /locus_tag="NBH55_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1509733.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="NBH55_001750"
     CDS             complement(2060..2404)
                     /locus_tag="NBH55_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCD1509734.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="NBH55_001751"
     CDS             complement(2558..3478)
                     /locus_tag="NBH55_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD1509735.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="NBH55_001752"
     CDS             3665..4156
                     /locus_tag="NBH55_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCD1509736.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="NBH55_001753"
     CDS             complement(4180..6555)
                     /locus_tag="NBH55_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCD1509737.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="NBH55_001754"
     CDS             6881..7507
                     /locus_tag="NBH55_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1509738.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="NBH55_001755"
     CDS             7530..8234
                     /locus_tag="NBH55_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD1509739.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="NBH55_001756"
     CDS             8426..9259
                     /locus_tag="NBH55_001756"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCD1509740.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="NBH55_001757"
     CDS             9456..11384
                     /locus_tag="NBH55_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCD1509741.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="NBH55_001758"
     CDS             11522..11977
                     /locus_tag="NBH55_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCD1509742.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12447..12716
                     /locus_tag="NBH55_001759"
     CDS             12447..12716
                     /locus_tag="NBH55_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509743.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            13116..13370
                     /locus_tag="NBH55_001760"
     CDS             13116..13370
                     /locus_tag="NBH55_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCD1509744.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13459..14505)
                     /gene="holA"
                     /locus_tag="NBH55_001761"
     CDS             complement(13459..14505)
                     /gene="holA"
                     /locus_tag="NBH55_001761"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCD1509745.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14543..16816)
                     /locus_tag="NBH55_001762"
     CDS             complement(14543..16816)
                     /locus_tag="NBH55_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCD1509746.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16809..17348)
                     /locus_tag="NBH55_001763"
     CDS             complement(16809..17348)
                     /locus_tag="NBH55_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCD1509747.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17484..17702)
                     /locus_tag="NBH55_001764"
     CDS             complement(17484..17702)
                     /locus_tag="NBH55_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCD1509748.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17699..17944)
                     /locus_tag="NBH55_001765"
     CDS             complement(17699..17944)
                     /locus_tag="NBH55_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCD1509749.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(18060..18788)
                     /locus_tag="NBH55_001766"
     CDS             complement(18060..18788)
                     /locus_tag="NBH55_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCD1509750.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5799 a         3654 c         3318 g         6117 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaat aaaaatctag
    12901 tttggtttga cgctattgcc tcccacaata gcgtcttttt gatttttttc agcttgtact
    12961 atccgttttt actgaaaaaa aatcctaata aaagactgtt aaggaaaaaa acttgtcatg
    13021 ttcatattgc aaagcacgct gcctttaggt ataataatga atgttgaatt atgcggtaac
    13081 acgcgtaaga acttaagtta tcggaggtga aactaatgcc aaacattgaa tctgcaatca
    13141 aacgtgttcg tacaagcgaa aacgctaatg tgaaaaattc atctcaaaca agcgctatgc
    13201 gtactgcaat caaaaaattt gaagatgctg tagcttcagg tgctgacaac gtagatgcgt
    13261 tatataaaga agctgttaaa gctattgata tggctgaatc aaaaggtcta atccacaaaa
    13321 acaaagctaa ccgcgacaaa tctcgtttaa gcaaaaaaat cgctaaataa aagaatcggc
    13381 ttcggccgat ttttttattt gtcaaaaaaa ggacaaaaac aaacgacctt cccgcatgct
    13441 ttgccctcgt ctttcttctt atcgatttct taacaactgc cctgtgcgca aaacgaatag
    13501 ttgaaaactt aattcttttt cgatttgtcc cgttttgatt tgataatcca tctcaactaa
    13561 ttgatcaaac aactgtacca gcaaatgttc atcgaatttt tttacttcct gcatggcaag
    13621 ttttacacga tatggatgga ttttcaatgt ttcagcaata tttgcctgct gataaccaat
    13681 ctttacaaga aattttgtct gtaaaaataa acgtaattga gaaagcaaaa tcgcattaat
    13741 tttgattgtt tcttcccctt gcataactaa atcttcgtat aaacgcaaag cttgttccgt
    13801 cttccctttt aaaatatatt gggtcatgtc aaaaatatta tgttctaacg ttttaggaac
    13861 taattcttct acttctctta ccgtgatttg ctgttgattt tgtccataaa ggaccaattt
    13921 ctgtaactct ctcatcattt tggataaatc taagtcggtc aacctcaaga ataaatcaat
    13981 tgcacttcgt tccattttga ttgaggaatt ctctaaggta ttcatcagat attgacgtac
    14041 ttctttttca ttcaactgtt ggacattgat gataattgct gtttttttca attgtttcgt
    14101 tacttttttc cgttcatcca acttttcata tggcgcaaag aatactaata cagtcgattc
    14161 taatggttct ttcaaataag ccgtcaataa atcagtattt tgttcgattc cattgtttac
    14221 tttttcacct gttaaaaaat aaggattctc tacgaatact aaccgttgat ctccgaaaaa
    14281 cggcaacgtc tctgcttcag ctactacctc atctaaactt gcattttcca tgtcaaaact
    14341 taaaaaattt aagtcatctt cgccctctaa ttggattttt ttgacaattt ctgtccgaac
    14401 ttgatcttgg agaaactttt ctgttcctaa aatcaagtaa caagatgcaa gtttttcttt
    14461 acgtattttt tgtaactctg cttgtatact caaattactc accactttca aaaattatcc
    14521 taccatgagc agatgagaat tactagtcta tcattgtttc cactagccca tgaggatgaa
    14581 aaactggatg ccagcgatag gtgatcattc cttgttgatc cgttcgccat gtttttatct
    14641 gattttcttc tagtgtctcc aatacttcgc tgtgcgggtg accataacga ttatttacgc
    14701 cgcaggatat aatcccatgt tttgcttcaa tatgggaaag aaaatctgtg ttacttgacg
    14761 tacggctacc gtgatgacct aatttgacta catcggcttt cagattagga taaatcaaaa
    14821 gcaattgttt ttccattgct tgattcaaat ctcctaaaaa taaaaagcgc ctattgttat
    14881 attccatcca tagaccaatc gaatcttcat ttcttccctc tcccttttca aaaggagtca
    14941 atacataaag atcataataa ccgctgatcc tgtccccatg tttgatttca atgatctcag
    15001 taccattttt ttgagcttca gataaatgcc tttgtaatgg ctcatttcgt ccagcgcccc
    15061 aaccgatata aatttttttg atagtaaaat gctgagataa cgtgggtaat tctcccacat
    15121 gatccgtatc gtcgtgtgtc aagatgagtt tatcgattac acttatcccc tgacctttta
    15181 gatatggaac gatatttctt tcagcaggag gacgtatcat accttgcgcc cacttttctc
    15241 tttcaaagtg taactgacct ccagtatcga tcaaaatcgt ttcttttcga aaaggacttt
    15301 gcaaaaagat actgtctcct tgaccgacat cgataaaagc gatcgttgaa gaaaagcgta
    15361 aaaatggcaa agtcaaaaaa ataaaagaaa aaagaccaac ataaaacaag tgtttcacag
    15421 gttgttcttt caacctatcc atacaaatca gtaatccgaa aatatacatc ccagatagca
    15481 caaggggcaa acgtcctata atgagagacg accctgaaga aaaatcgatt attcgctcaa
    15541 ataagattaa tccctcttct atcagcaaaa gtaaagtttc cggcagccag cgattgaaga
    15601 gtgtgagaaa taacacacca ggaagaaaaa gaaacttgaa taccggtgca atcagtaatg
    15661 taaaaagact acctaaaata ggccactcat aaaaatgcca tacaatcaac ggcatcgtga
    15721 tcatagtaaa caggacgtga gaggccgttt ttttcaacca atctccatca aaagaaatca
    15781 ggataaacaa aaaactcatg aaaaaactaa gctgtccacc cgcatgaaaa agaaatcgag
    15841 gatcaaatag taaaagaagc caaaagacaa ctgcaaataa gtccatggtg gaataatgct
    15901 tattcagcaa tttgactagc aatcgcaata aaaacagcaa actagcacgc aaaacagaaa
    15961 tagaacttcc tgcaatagaa acataacaga tagttagtag actcaacgga ataatactga
    16021 cgtgtagtgg ccatccgata cgtcgaaata aataatacag ccaccctaaa taaaattgga
    16081 tgtgtagacc cgatagactg aacagatgca gcaaaccact ctccctaaaa atttcttctg
    16141 gttctctgaa tcctgcatct ttatagcccc ataataaaga attaatatat acacttgttt
    16201 tttgcggaaa ttcagattgg acaaaggaga taaaagatcc tctttttctc tgaagccagt
    16261 ttcgccagtg attttgcatg gtgatctgtt caatcttttg aatttggaac cgtccagaaa
    16321 ttctatggtt cctgaagtaa gcttcttggt caaaattata aagattccgc ttttttgctg
    16381 gagagatgaa ctcgccttca gcagtcaatt ctattccctt tcctcggaag ttaagccatg
    16441 ttttcaattc tgcttctgtg tttacttgat aagaaagctg gagcttttgt ttattctctg
    16501 ataaccttcc ttccattgtc aaaaaattac catttgcacg aatcgtatct gtaaatacag
    16561 taatttgcgc agatgttgtt ttttcctcta aaaatgaata ctcttgtagc ctataagaaa
    16621 tggataccgt tatgctacac aaaatacaca agtacgcgat aaaacgtcct tcttttagcg
    16681 aaaaccataa aaaaattccg gcaagaagca aacgataacc ccaattgtca gtgtaataca
    16741 acaaacagat aatcatcgac gtaattgccg gaaaaatcaa ccgatttcga acatgccttt
    16801 ctaaaacgct agacatgttt tcctgttaat tcattttgaa gttttgaaaa atgctgtgag
    16861 tctaaagcaa cttggtgtac agatacacct aattgttcga tcaacgcttg ggcataagga
    16921 tcatttcgat agtcatggag ataattgatc cctttcacac cagcttgcaa caaagccttt
    16981 gtacaggcca aacatggaaa atgtgtgaca tagatttctg aaccttccgt cgggatacca
    17041 agttttgcac actgtaggag tgcattcatc tctgcatgga tcgttcgcaa gcagtgtcca
    17101 tccacgacgt aacacccttc atctatgcag tggacatctc cactgactgc tccattatag
    17161 ccgccagcaa tgatcctttt ttctcgaacg agtgtcgctc ctacttctaa acgtgtacac
    17221 gtactacgta aagataacag tactgcctga gccataaagt actggtccca aggaatgcgt
    17281 tcattatgtg acttatcagt aactttttct ttattcgtat cgcatgcttt tgaagatttt
    17341 tctaacatgt tttatattcc tccgtttttt caagttgatt aaaaagtact cattgcttaa
    17401 acaaaatatt ctttaaatat aacgaatcat cctgtctgac aaaagatcct acccttcact
    17461 gccgaaatga agtccgtcat ccattagccc attataaaaa ttcttcgaat ccatttgttc
    17521 aagaaattcc gtataaaaat cgatacttca caatcatggc gttttgctac ttctgtgaga
    17581 acagagatat attctttgat caacttgtta ttccgatatt tttgttttgt ctcatcgact
    17641 gctggtggcg tgataaagaa agcatgatca gaactatagt cttttttcag ctgaactatc
    17701 atccactgca gattttttgg tacgtttctt tagaaagttg tttatgctct gctagatcat
    17761 tcgtcccaat gagaataaat actttatctg ctgtatagct aagtacatct ttggcaaagc
    17821 gttctcgtag agcttgactg ttattgccgc tgacagccgt attcataata tgaatcccag
    17881 atattttatt ttggagatag acattcagca ttggtttttt taatgtttct ttgcgaacaa
    17941 tcaagctatt tcctatccaa aaatactacc tcctcgtcct gccaagctct tagaattgga
    18001 ttattcttag tatccattag cgtccattta ttgctcgtgc cagtcgattt tgaatcattc
    18061 tatttaattg taacgaagtt ttttagtttt tcaactgtct tttcaccgat accagaaatc
    18121 tcctgcaggt cttccacttt ctggaaggat ccgttttctt ctctaaatcg aatgatttct
    18181 tgcgctttga ctgatccgat cccaggaatc tgctgaagtt ctgatggagt ggctgtattg
    18241 atatttattt tgggaggctc ggaatctgct gtaggctgtt ttccttccga ggatgccaac
    18301 tgatcaagat gttctggcag ctcttcacct acagcaggga tatataggac ttgctgatct
    18361 tctatcttcg cagaaaagtt gattcgatcg ctatccgctt cttctaataa tccacctgca
    18421 cgctttagta catcatagac ccgttcttct gaagaaaagg aatagatacc aggctccttc
    18481 acacttcctt tgatatcggt gtacatgacc tttggacgag catcctcttc tacatcgttt
    18541 ccttcagctg atggatcttc tccactggaa ctaaacgaag aaagtatttc ttttgcagca
    18601 gtctcttctg tttctctata aaatagcaaa tttgataata aaaaaccact tgcgatacaa
    18661 aagataagaa ctatcataga catccaaatg atcaatttgg gtgaagctag tattttcttg
    18721 ctgatttttt ctatcattgt tcttttttca aagaatgacc agtaaaaatc attcattact
    18781 cctttcattt aaaattgaag aaacagtgag ctctggcaaa atcgtccctt caacatctaa
    18841 ttacgtcaat tttttctttt gtttttattt ttatcgtgaa gatttttg
//
LOCUS       DAHHPA010000056        18443 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.057, whole genome shotgun sequence.
ACCESSION   DAHHPA010000056 DAHHPA010000000
VERSION     DAHHPA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.057"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            171..515
                     /locus_tag="NBH55_001767"
     CDS             171..515
                     /locus_tag="NBH55_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCD1509751.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="NBH55_001768"
     CDS             516..935
                     /locus_tag="NBH55_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCD1509752.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1027..1377)
                     /locus_tag="NBH55_001769"
     CDS             complement(1027..1377)
                     /locus_tag="NBH55_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509753.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="NBH55_001770"
     CDS             complement(1343..2674)
                     /locus_tag="NBH55_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCD1509754.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="NBH55_001771"
     CDS             2930..3496
                     /locus_tag="NBH55_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1509755.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="NBH55_001772"
     tRNA            3641..3728
                     /locus_tag="NBH55_001772"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /gene="bsh"
                     /locus_tag="NBH55_001773"
     CDS             4062..5036
                     /gene="bsh"
                     /locus_tag="NBH55_001773"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCD1509756.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="NBH55_001774"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="NBH55_001774"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCD1509757.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="NBH55_001775"
     CDS             complement(7451..7897)
                     /locus_tag="NBH55_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCD1509758.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="NBH55_001776"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="NBH55_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1509759.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="NBH55_001777"
     CDS             8362..8880
                     /locus_tag="NBH55_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD1509760.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="NBH55_001778"
     CDS             complement(8957..9913)
                     /locus_tag="NBH55_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1509761.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="NBH55_001779"
     CDS             10079..10885
                     /locus_tag="NBH55_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509762.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="NBH55_001780"
     CDS             10882..11871
                     /locus_tag="NBH55_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1509763.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="NBH55_001781"
     CDS             11868..12872
                     /locus_tag="NBH55_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1509764.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="NBH55_001782"
     CDS             13004..13546
                     /locus_tag="NBH55_001782"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCD1509765.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="NBH55_001783"
     CDS             13566..14264
                     /locus_tag="NBH55_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCD1509766.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="NBH55_001784"
     CDS             14285..14497
                     /locus_tag="NBH55_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCD1509767.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="NBH55_001785"
     CDS             14497..15459
                     /locus_tag="NBH55_001785"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCD1509768.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="NBH55_001786"
     CDS             15477..15872
                     /locus_tag="NBH55_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD1509769.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="NBH55_001787"
     CDS             16035..16400
                     /locus_tag="NBH55_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCD1509770.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18208
                     /locus_tag="NBH55_001788"
     CDS             16397..18208
                     /locus_tag="NBH55_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCD1509771.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18270..18437)
                     /locus_tag="NBH55_001789"
     CDS             complement(18270..18437)
                     /locus_tag="NBH55_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCD1509772.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         5925 a         3125 c         3809 g         5584 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggcaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaagaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attattcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 cacacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcaac caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatct cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt tagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga ttttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaattga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggc aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaaaga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgccta
    17521 tcagaatgag cccttttcag aagctagccg aatatttatt gaagggcaga taaaagaagc
    17581 ggaaaaaggc aattgcacag gagcatttgc ttcaacattt gatgcattaa aagactggcg
    17641 tgatccaatc cggcaggcta ttgaatggca cgttttttct gctaaggaat acaaagaaat
    17701 actatgggga tggtttactc atctaaatag ctttttaacg atcggtccgc caatcattcg
    17761 gacgaaagaa ttagcagcac tgatagatgc gggtattttt catttgatcg aaccgccatt
    17821 tgatattcag caaaagcaca accgctatgt tatttctcta caagataaag aaatcagcag
    17881 tcgctatttg attgaagctc gattgccaca aacaagcttt tctcgttcaa gtaatcctgt
    17941 cataaaaaat ttgaaggaaa acaaacttgt tcgccctttt tcattttcag ataatacagg
    18001 aagttatcat tcagaagcta tagatgttga gcgaggtacg aatcgcgtga ttgatgcttc
    18061 aggggaaatc cagtctgact tttattgcta tggaatacct gttgaaggtg tggattggct
    18121 aactgcagca gtagctagac cttatacaga tccatggaat ctgagacagg cagataaaat
    18181 agcacaactt atattgactg acaaataaaa atacgcatga tgacttatct gttttttaga
    18241 aagtcttcat gcgtattttt aaataattat taacgagaaa cgcgtttacg cattgctttt
    18301 ttacggtttt cttcgatcat tgcttctttt tcgtcaattg gatcgatcac tgtttttttc
    18361 acagaagcaa ccaaaccagc aacggcagcg gctgttgcca gacttcctac taatattcca
    18421 gatacaaatt ttttcattga atc
//
LOCUS       DAHHPA010000057        18431 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.058, whole genome shotgun sequence.
ACCESSION   DAHHPA010000057 DAHHPA010000000
VERSION     DAHHPA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.058"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            4..2376
                     /locus_tag="NBH55_001790"
     CDS             4..2376
                     /locus_tag="NBH55_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509773.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="NBH55_001791"
     CDS             2437..3897
                     /locus_tag="NBH55_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCD1509774.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="NBH55_001792"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="NBH55_001792"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCD1509775.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="NBH55_001793"
     CDS             6128..6277
                     /locus_tag="NBH55_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509776.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6954
                     /locus_tag="NBH55_001794"
     CDS             6358..6954
                     /locus_tag="NBH55_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCD1509777.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            6967..7866
                     /locus_tag="NBH55_001795"
     CDS             6967..7866
                     /locus_tag="NBH55_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCD1509778.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            7869..8354
                     /gene="ssb"
                     /locus_tag="NBH55_001796"
     CDS             7869..8354
                     /gene="ssb"
                     /locus_tag="NBH55_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1509779.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            8720..8980
                     /locus_tag="NBH55_001797"
     CDS             8720..8980
                     /locus_tag="NBH55_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509780.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9427..9633
                     /locus_tag="NBH55_001798"
     CDS             9427..9633
                     /locus_tag="NBH55_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCD1509781.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9633..9857
                     /locus_tag="NBH55_001799"
     CDS             9633..9857
                     /locus_tag="NBH55_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509782.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            9872..10309
                     /locus_tag="NBH55_001800"
     CDS             9872..10309
                     /locus_tag="NBH55_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509783.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            10302..11009
                     /locus_tag="NBH55_001801"
     CDS             10302..11009
                     /locus_tag="NBH55_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509784.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            11390..11569
                     /locus_tag="NBH55_001802"
     CDS             11390..11569
                     /locus_tag="NBH55_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509785.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11662..11847
                     /locus_tag="NBH55_001803"
     CDS             11662..11847
                     /locus_tag="NBH55_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCD1509786.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            11881..12312
                     /locus_tag="NBH55_001804"
     CDS             11881..12312
                     /locus_tag="NBH55_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCD1509787.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            13249..14523
                     /locus_tag="NBH55_001805"
     CDS             13249..14523
                     /locus_tag="NBH55_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1509788.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            14577..16004
                     /locus_tag="NBH55_001806"
     CDS             14577..16004
                     /locus_tag="NBH55_001806"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCD1509789.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            16017..16889
                     /locus_tag="NBH55_001807"
     CDS             16017..16889
                     /locus_tag="NBH55_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD1509790.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            16984..17979
                     /locus_tag="NBH55_001808"
     CDS             16984..17979
                     /locus_tag="NBH55_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1509791.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
BASE COUNT         6659 a         2939 c         3760 g         5073 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agagttgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaat caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgttacga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgag
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcatgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatt gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat aaaacagggc agacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaattttc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg aaatctacca acaagagatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggtagac agcaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaagctaccc gaaaagatgg gtattttgag atccaagatc gattgttttc
     4021 aggagaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgc gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagatg cagaagagac
     4381 ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc gattggttga tcggcatgaa
     4441 cggtagccta ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttacaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa atcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcaat ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 aaaatatcta agctatccgc gaacggatac cccttatatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttaaaa gccgaagcga ttccgactcc
     4981 tattcataca ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc cactgcagca
     5101 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ctaaagacag tcggaaaagt
     5221 accagtagat ctaggttgga aaaagctgtt tggcaaagag agcgacagaa aagaaaagga
     5281 agaagaaccg ctacttccta aagtgacaaa aggagaagcg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa ggtacgttga tcacagcgat
     5401 gaaaacagct ggaaaaaccg tggatagcga agaagcacaa tccatattga aagaagtaga
     5461 aggaatcggt acagaagcaa cacgagcaaa tattattgaa actttgaaac aaaaggaata
     5521 tatcaaggta gaaaaaaata agcttgtggt gacaaataaa gggattttac tttgccaagc
     5581 cgtggaaaaa gagccgctgc taacaagtgc cgaaatgacc gccaaatggg aaagttactt
     5641 attaaagatt ggtgagcaga aaggaacaca agctactttc ttagctaata tccaaaaatt
     5701 tgtctctcac ttgttggaag tcgtaccggg acaaatccag tcaacagatt ttggatcgac
     5761 gttacaagag gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt tagggatttg
     5821 tccaaaatgc caggaacaag aagttttact ctatcataag gcggccgcat gtacgtctga
     5881 agcttgtgat tttagattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggaaatc attcaaaatg gccgaacaag ccagccagtg aaaggattaa aaggtcaaaa
     6001 gggttcgttt gaagcaacga ttgttctaaa agaagacttc acaacgagct ttgaattttc
     6061 tgaaaagaaa aaaacgaact ataagaaaag aacacgccgc actacaaaat aatggagggg
     6121 ttatcttatg cgaaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagcaaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa ttgaattaga tcgataaaat cttcctggct attgtatacg
     6301 cataaactta tgttatacta tatttataag tttatacgta tacaaaggag ggagaaaatg
     6361 acaaaagcaa tggtttacaa gctgctagaa gaatttaacg gaactttggc tttgaaggat
     6421 gccaaaaaag cgggcatttc tcctgtaacg atcaaacgta tggtagatcg tggagagctg
     6481 gatcgtgagt atccaggttt tttcacctta cctggtcaat ttcctgatga attgtttatg
     6541 gcacaaaaga aatatgagcg cggcattatt tcgcatatca ccgcgttgga tttatatgat
     6601 ttaacggata tgatccctag acagatcgat ctaaccgtac cttacggtta tcacgtttct
     6661 gaaaaggggt tgaaagaatt cgcagttgag ttacactatt caaaagcgga atggtatgaa
     6721 ttaggtaaga tcgaaataaa gagtcgttat gggaatcctg ttatagctta tgatccagaa
     6781 agaaccttgt gtgacatttg gaatccctgg tacaacgttg aggatgaaat caaagtaaaa
     6841 gcaatcaaaa actacatgga gtcggacaga aaaaatctta gaaaattgaa tgagtatcga
     6901 cgaattttgc ctacagacaa aacgatacgc tcttatatta tggctttaaa ttaaaggaga
     6961 agacacatga cacctacgca attaaaagca aaagtgaaga atatttccag agaaaaagaa
     7021 gttgatccgc agcttgtttt aagacatttt atgatggaaa aattcttgga aaaaatctca
     7081 gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttaattgg ctcaaagtat
     7141 ggcattgaaa atcgaacaac aaaagacatt gatacgactt tacgggaaat gaaagtaacc
     7201 aaagaaacat tgacaacggt tctaaacgat attttttcaa cgccgacgaa agaaggcatt
     7261 caatttgaga ttcaaggaat gaaagaaaca agggaagctg attattatcc agggtttagc
     7321 ttacgtgtac tggcacattt ggaaaacatg cggccagatt ttaaggtaga cgtgacgaca
     7381 ggtgactcga tctatcctgc aacgatcact cacagtcata aattgatgtt tgaaaatcga
     7441 acgattcctt tggaatctta tcctacagaa caaattattg ccgaaaaatt aagtgcaacc
     7501 tttgattttt taacagataa ttcgagagga aaagattttt atgatctcta tactattcct
     7561 aaaatggaaa agatcgatga gaagaccttg aaagatagtg tgagaaatac atttacacgt
     7621 cgaggaaaaa ctgatccgtt aaagatttat tacgaaaagg atatggatgt actgaaaaat
     7681 agtcatgagc taaaagatac ctggaagaaa tatcaaaaaa gcaatcctta tgctgcgtcg
     7741 atcacgtacg aagagacaat ggattcgatt agtaatttga tggaaaaagt gattcacgtc
     7801 gaagaaaaag agcgaaaaat agcttatcaa agacaccaac agatgaacag agaaatggag
     7861 agataaaaat gatcaataat gtaactttag taggacggtt aaccaaagat ccagatttac
     7921 gttataccgc aagtggcaca gcagtggcta cttttaccct agccgttaat cgtaatttca
     7981 cgaatcaaaa cggcaatcga gaagctgatt ttatcaattg cgtgatttgg cgcaaaccag
     8041 cagaaacgat ggcgacatta gctaaaaaag gcatcttgat cggcgttgtc ggacggattc
     8101 aaacacggac ttatgacaac cagcaaggac aacgtgtgta tgtgacagaa gttgtagctg
     8161 ataacttcca actattagaa agcaaagcag cgacagagag ccgtgcgcac gctgatcaat
     8221 cgtccacgag tccaagcacc accacttttg aacaacggga tacagcgacg cctaacaaca
     8281 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8341 atctaccgtt ttaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8401 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     8461 tttcttttac gtttctatta taagaattaa cgagtaggct tactgtcttt cttggcaaat
     8521 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     8581 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     8641 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     8701 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     8761 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     8821 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     8881 tattgccgtc acaggtcact tcaataaacg aaatcagctg gttattgcta gcatgtatgt
     8941 tagaaatccc gatcatttta cacgatcgat gggcatataa ataaaagaag agttgtagtc
     9001 ctaaatgagg attgcagctc tttttgtttt acatagaaac gggaacagga aaaggcctgt
     9061 ttaggaaggc acaagcgcct tctcaattaa aaaacctttc tctcactcca accgccaccg
     9121 ccgttcgaga agaaaggaag tgaacgctgc acgttcacta agatatggtg tcttgcaaag
     9181 caagacaaaa taggaaaagc ttcctctcac caaaaggttc gttcctgttc gctatctctt
     9241 tattgatttg ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag
     9301 ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt cgttgattca ttctggccaa
     9361 caaatcaata aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa
     9421 tcaatcatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt attcccaaac
     9481 tatcatgcac cacatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc
     9541 tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattac
     9601 attttgattg atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgaatg
     9661 taaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
     9721 tagaaacccc tttagtagag gacgcaccga ctatgctatt tgaagaccta gtgaagattg
     9781 caggatatga actgcattat caagaaaact tggaaactta tgaaaacgaa tattggttaa
     9841 ccaaattaga gaactaaaaa ggagaaaaaa tatggctcag aattactatg acgaatttgt
     9901 caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc tataccatga
     9961 aacacgtgta cccaaaaaac attacaagga aaaattgtca gtcgcagtgg aagaaatgat
    10021 tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacattgg aagaactgaa
    10081 aaaacaaaat ggaaaatggt ttttccaagc cttactatgc ttggaagcag gcgtcaaacc
    10141 aagcacaatc aaaccttctg aataccaagc tttagaacta acgtatggaa aatttatcga
    10201 aacgaaaaaa gccaagactg tttcaagtga atggttggac tactttgaaa atgtcaacaa
    10261 gtatggcgca tgttacatca tgaaaaagga ggacaacgaa aatgagtaat gtggtttatc
    10321 aactaacagt ggaagatgtt tctcactttt taacaaacaa agaacatttg aatgaagtag
    10381 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag
    10441 tggaagaaca gttgtatatg gaaagcgaaa cgagtcaaac gaaggtcaca gtcagccata
    10501 atcatttaga cgtgtataaa cgtcgtcctg aaacggtaaa aaaaggaaca gagcttctac
    10561 gactagaaac agaaaaatac atcatgaatg tgtacctaga aaggagtgag ctggtcgaaa
    10621 actttgtcag ctacacgatt tgtaggaaac actattactc agaatcagaa tattattcaa
    10681 tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc aaaaatgcga
    10741 cggtagacga ttattacctt gtatttacta gatttctgac gtgtgggacg gaatctatcg
    10801 tgttaatggg aatggattat gaactcgctg tacccgaagc atttttgagg gaatatgtag
    10861 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagatacct
    10921 acacttacga cgatacagaa gaaatccaaa atgagcttga ctgtaaacga atgccatatc
    10981 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag aatgtgtgaa
    11041 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa
    11101 ccgcccccgc cgctcaggtg gtgcgaaata agcgggcaac gaccgataac ctaagagaaa
    11161 gaaggaaatc catatgaata aaatcattcc tctgttgttc gtactgtttg ttggcgagat
    11221 acttatgcgt catgctgcag ctgtacattt aaacttcgta gtattcttag gaatactggt
    11281 attttgtttg atcttttggc gatttgtatt agcccttgtg agtctactat tcaagatgat
    11341 tgcagccata tttttcatag ggttatttcg atgaaaggag agagtactaa tgccaattgc
    11401 aataggaaat aaacgtttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagga
    11461 actcaaacag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
    11521 gattgcacaa gaaaaagcag ggtttgatgt accagcactc aaaaagtgac gtcacttttc
    11581 atttgaagaa acaaaagtga tagcacttta cgcacgtgtc gatacgtgtt atgattattt
    11641 tgtaaggagg tatagtgcac aatgccactt acgggaaaag aaatgcttaa gttattgaaa
    11701 aaaaatggct gggttgagcg aaggcaagag ggatctcatc atcatcttta taaggatggt
    11761 gttagaatca ctgttcctgt tcatgccaat caagatcttg gccgaggact tgagcaaaaa
    11821 attttaaagg atgcgggatt gaaataatct cgcattcgat taaagaaagg attgactgaa
    11881 atgttaagtg agcctttagc aaaaacctat cctgctattt ttagcccaga agaaggtggt
    11941 gggtacttta ttgaatttcc tgatgttcaa ggtgcttaca caggaattaa cgaagacgat
    12001 atatcttatg gaattgctat ggcacaagag gtgttaggta tggttttagc agattatatt
    12061 gagcatgaag atctacttcc agaaccaaca ccaatcaata agatttctgt tgaagatgat
    12121 tcttttacta ctttaattcg tgtagatgtt gccaaatatt tgaaagacac agaacttgtc
    12181 aaaaaaacct taacaattcc tcaatgggcg gataagctag gcaaaagggc tggcataaat
    12241 ttttcagtcc ttctaacaga atcaattgca gataaagcag ataacattct tcgttctgga
    12301 agaaataatt agaactcaaa agagtcagaa acttaaaaat ctttggcttc tttttttact
    12361 acaaagatgg cttctttaaa ttatttgcct gattgattta aaaaaattgc gcaaagacta
    12421 cgagctaatt aagttgctcg tggtctttct ttatgaaaac tttttagatc tagataaaaa
    12481 cattgagaca ctttgaaaaa tttttttaat agtaatgggc gcttctatgt caagctgatt
    12541 cttctttcgg taaattaatt ttacttagaa tatcactatc ttgataaaaa atggtgtatt
    12601 cacaaattct ggcataaaaa atacctactt tctagttaag gttcctgaat aagtcgcaat
    12661 ccaattattt ccagctgatt tataactttt taaatataat tttccataat accaataacc
    12721 attacttttt tctgtccaat ttcgttattg agaagatcta gaaggccagc caactttatc
    12781 aaaaatcatc attcctgtca ctcgtatcgt tttagaagct gctcgagtaa gtaatggaga
    12841 aactgttaaa taattttaag taacagttaa gtcgttagtt gggttatcaa tggctattcc
    12901 gtccttatct gaacctcgaa acccaatcat agaaaaagtt ttttgaacaa gttttaacag
    12961 ataaatcaag ttatatacta gaaaattcgt aaaatatatt tttaatgtca taatcgtttg
    13021 atttttctgg attgacattt atttataaaa aatagtgcca ttaaaaaaga aaagtgacac
    13081 accaataaaa gtcatatcct gtgacattat atggcattat ccaaatagaa taaccggttg
    13141 acatgtaagc ggattccttt tataatttta ggagtataag tccttttcta aaaatattaa
    13201 ccggttatca tgaaagggga tacaaatata tcgttggagg ataaaaatat gtcaaaggtt
    13261 aacgtattta aaaatcaatc ctatttacaa agttcagcta cattattact attttttgct
    13321 tcttggggtg tttggtggtc attttttcaa ctttggctaa catctgaatc aaatggttta
    13381 gggttatctg gcagtgctgt aggaacagta ttctcggcaa attcgttagt taccttaatt
    13441 ttgatgttta tttatggaac attacaagat aaattgtata ttaaacgaaa tttattaatt
    13501 tttgcttctg tattagcgac acttgttgga ccatttttta tatggatata cgggccattg
    13561 ctagataaca attttaattt aggcattatt atgggagcac tatttttgtc agctggatat
    13621 ttagcttctg taggagtttt tgaagctgtg tcagaaaggt ttagtcgttt atttggcttt
    13681 gaatatggac aagcaagggc gtggggatca tttggttatg ccttggtagc gcttttggca
    13741 ggatttttat ttgtaaaaaa tcctcattta aacttttggg cgggatcttt ctttggttct
    13801 ttactattgt taaatttatt attttggaac cctaaagttg aacgggaagc aaatcaaaat
    13861 tttaatcaag aacaagctga atcaaatagt attccttctt taaaagaaat gtttgatcta
    13921 atgaaactgc ctcaattatg gacgataatc atctttattg tttttacatg gacattttat
    13981 acggtattcg atcaacaaat gtttccggga ttttatactg gtttgttttc aacatcagct
    14041 aatggtgaaa aaatatatgg gacattgaat gctattcaag tattttgtga agcgttaatg
    14101 atgggaattg ttccaatcat tatgagaaaa ttaggggttc gaaatacttt gttattaggt
    14161 gtaaccatta tgtgtgtacg aattggattg tgcgggtttg cctcgacacc attatctgtt
    14221 tcatgcataa aaatgttgca tgctttagaa gtaccattat ttacattacc aatgtttcgc
    14281 tattttacac ttcattttga tacaaagcta tcagcaaccc tctatatgat aggatttcag
    14341 atagctgctc aaattgggca agtgatttta tcaacaccat tgggaatatt aagagacaac
    14401 gttggctatc aaccaacatt taaaattatt tctcttattg tattactagc aggcatatat
    14461 gcattcttta ttcttaaaca agatgataga gatgttcaag gggatccatt tattcgagga
    14521 taatggaaac caacagaata aaaaacaatt ttatctagaa agtgagcgaa tatattatga
    14581 caaaaagagc tatagaatta acaaatacac gttatcgttt gggatatcat gtgtctgcac
    14641 catcaggatg gattaatgat ccaaatggat tttgctattt tgatgggtat tatcatgtgt
    14701 tttatcaaca tcatccatat agtgctgaat ggggaccaat gcactgggca catgctagaa
    14761 gtaaggattt agttcattgg gaatctttac cacttgcttt gactcctgga gatcaagaag
    14821 atgaaggtgg ttgtttttca ggaagcgcta ttgaaaaaaa tggtgtgttg tatctgttct
    14881 atacaggaca tcactattat ggagacaatg atccagatca tttttggcaa aatcaaaaca
    14941 tggcgtatag tacggatggc attcacttta ctaaatatga aaaaaatcct gtaatagcaa
    15001 aagcaccaga agataacaca catcatttca gagatccaaa agtatggaaa cacgatgaaa
    15061 aatattacat ggttttgggt agtcaagaaa aagatggatt aggtcgtgct attgtttatc
    15121 agtcgttaga tttattaaat tgggattaca ttggaccaat ttctaaagcg aaggaagtac
    15181 ttacagaagg atttatgtgg gaatgcccag acttttttga attaaatgga aaatctattc
    15241 ttttattatc ccctcaggga attgaagcaa atggaaaaga ttatttaaat ctatatgaaa
    15301 caggctactt tatcggtgat tatgattatg agacagcaaa attcattaga ggaaattttt
    15361 atgaattaga taagggacac gatttctatg caacacaaac aactttatct ccagatggta
    15421 gacgtattgt tatggcttgg atggatatgt gggaaagccc tatgcctgaa aaagtagacg
    15481 gatgggcagg agcattaacg cttcctcgag aattagagtt aattggagat catttgtata
    15541 tgaaacctgt gagagaatta gaacaacttc gagttggcag cggcgttgaa acaaaagtta
    15601 atttggcagg agaaataata atcgctcaag atgcttcatt caaagaagct ctaattgatg
    15661 tactattgac taaatcaaaa aatcaagaaa tcaaattttc attaaaaaca aaagatgaag
    15721 agttaattaa tattttttat tctaaggata aaaatgaatg taccttaact cgtaacggca
    15781 aaaatgatcc aaggtatgga accattcaga tgtgtgatag attatcactt cgagtgttta
    15841 tcgataaaag ttcggcagag atttttatta atgatggcga gcttgttttt acagagcgtt
    15901 attatacaga aaatcaacca agtatgcatt tgagtgttag tgaagaagct tttgtctcga
    15961 gtacaattta tcatctaaaa aaagatgcaa tttcttacga gtaaggaggt tttatgatgt
    16021 taattggtag catagaagca ggtgggacta aatttgtatg tgccgtggga gacgaggatt
    16081 accgagtaca agaccaaata atttgtccaa cgaccacccc cagggaaaca ttgaaaaaaa
    16141 ctgttgacta ttttaaacag tttgatataa atgcattagg tattgcatca tttggtccaa
    16201 tagaaattcg aaaaaattca ccaaaatcag gatatattac gtctacacca aaggtgggat
    16261 ggcgaaatgt taatgtgtta ggttatttac aagatgagct gaatgttcca atgtcttgga
    16321 caacggatgt gaacggatca gcttatggag agtacatcat gtccacttta tcgaatgaaa
    16381 aaataaattc attagtttat tacaccgttg gtactggcgt tggtggtgga gctgttgttg
    16441 acggaaaact attaggatcg tttggtcatt cagaaatggg ccatacgttt gtaaaacgcc
    16501 atcctgatga tattgatttt aaaggaattt gtccttatca tggtgattgt ttagaaggct
    16561 tagttgcggg accaacgttt gaagcacgtt ttggaaaaaa gggtgaagac gtttctttaa
    16621 cggatcctat ttgggatgta atggcctatt atctagcaca agctgtgatt caaacaacat
    16681 tgattatacg accggataaa atagtatttg gtggaagcgt agtaaatgaa aaattgttag
    16741 aaaaaattcg tgttaatgtt aatgaattat tggatgatta cgtggaaata ccaccgttaa
    16801 gtaaatatat tacgcgtcct ttaattaaaa ataatggatc ggcaacacta ggaaactttg
    16861 ctttgggctt gaatcaatta caagaataaa acatagaaaa aatatcgatt atcaggtata
    16921 gtagttctat agagttcttt ttttcgatag ttaattattg cattatttag gagagacaag
    16981 agaatgcgcc caaaattaga agatgttgcc aaaagagcaa atgtatcaaa aacaactgtt
    17041 tcacgtgtat taaacaatcg tggctattta agtcaaaaaa cgattgataa tgtttataag
    17101 gctatagaag aactcaatta tcaaccgaac gttgtagcgc gtcaattatt tcaaaagaaa
    17161 actaacattg tcgggttact atttcctaca gtagccaatc ctttttttag tgaactcgtt
    17221 gaggcattgg aaaaaaagtt atatgaaatt ggttataagg tattaatagg caattcgatg
    17281 aataataaag aaaaggaaac aaattattta aatcagttat tgtctgatca agtagatggt
    17341 ttaattgttg gtacacataa ccaagggatt caggaatata aatatcagaa tttaccgatt
    17401 gttgcaattg atcgagtgat gaatgaagat attccagttg ttgaatccga taactacaat
    17461 ggtggaaaac ttgcaaccaa gttattaatt gcacaaggtg caaaaaatat tattcataca
    17521 aacggtccaa ttgacttaca gactccggct aatcgtcgga gactagccta tgaagatact
    17581 atgaaagcat atcagttaat tccacgaact gtgacattag atttcaatat ttcttatgta
    17641 aagaaaaaac agattttttt ccaaatgttt gaggattatc ctaaaataga tggtattttt
    17701 gcttcaaatg acatcgatgc ggccctaata ttacaagtag caaaagaaaa ggggttaaat
    17761 gtaccagctg atttattagt agtcggatat gatggtactt taatgactcg gagtatatta
    17821 ccagatctga caacagtaat acaaccaatt aatgatatag cagacacagc tgttgctatt
    17881 ttgatgaaac gaataaataa agaagaaaca aaaaaggaat atattttacc tgtcacatta
    17941 tggatgggga agacatcagt aaagaaaaag gacaattaaa tttataaaga ctatcaatga
    18001 taatttcatt attaattcgt gatataaaaa gttgttaaat ctcttataat agactatgat
    18061 acaaaatcat gcttttaatc tgtgtagatg ttgccaaata tttgaaagac atagaacttg
    18121 tcagaaaaac cttaacaatt cctcaatggg cggataagct aggcaaaagg gctggcataa
    18181 acttttcagt ccttctaaca gaatcaattg cagataaagc agataacatt cttctttctg
    18241 gaagaaataa ttagaactca aaagggtcag aaacttaaaa gtttttgatc ctttattttc
    18301 aagtctgatt tgacagattt tcggcagctc atttatttat ggtcagaaat agttgctgaa
    18361 attttaatct attagcaaat agtccagaac gtatgaagag gattcggata tcatttgtca
    18421 ttaacaccat a
//
LOCUS       DAHHPA010000058        17792 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.059, whole genome shotgun sequence.
ACCESSION   DAHHPA010000058 DAHHPA010000000
VERSION     DAHHPA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.059"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..1585
                     /gene="uidA"
                     /locus_tag="NBH55_001809"
     CDS             <1..1585
                     /gene="uidA"
                     /locus_tag="NBH55_001809"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCD1509792.1"
                     /translation="LPKKLLEERLVLRFGSATHEAKVFLNGKLINQHKGGFTPFESEI
                     NQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVNFDFFNYAGINRP
                     VKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDEEAQLIAEAKGKE
                     GVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGIRSIQVSNGKFLINNKPF
                     YFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYSEEIMRLADREGI
                     VVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKELIERDKNHPSVVM
                     WSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDICKVGDIVDVLC
                     LNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADTVAGLHDTVPTMF
                     TEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNKKGIFTRDRKPKM
                     IAHYLKKRWSSIPNFSYKK"
     gene            1757..2158
                     /locus_tag="NBH55_001810"
     CDS             1757..2158
                     /locus_tag="NBH55_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD1509793.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            2152..2397
                     /locus_tag="NBH55_001811"
     CDS             2152..2397
                     /locus_tag="NBH55_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1509794.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(2721..2942)
                     /locus_tag="NBH55_001812"
     CDS             complement(2721..2942)
                     /locus_tag="NBH55_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509795.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(3104..3176)
                     /locus_tag="NBH55_001813"
     tRNA            complement(3104..3176)
                     /locus_tag="NBH55_001813"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3141..3143),aa:Lys,seq:ctt)
     gene            3252..3482
                     /locus_tag="NBH55_001814"
     CDS             3252..3482
                     /locus_tag="NBH55_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1509796.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            3526..4695
                     /locus_tag="NBH55_001815"
     CDS             3526..4695
                     /locus_tag="NBH55_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1509797.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            5269..5838
                     /locus_tag="NBH55_001816"
     CDS             5269..5838
                     /locus_tag="NBH55_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCD1509798.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            5825..6685
                     /locus_tag="NBH55_001817"
     CDS             5825..6685
                     /locus_tag="NBH55_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCD1509799.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            6875..7579
                     /locus_tag="NBH55_001818"
     CDS             6875..7579
                     /locus_tag="NBH55_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1509800.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            7584..9419
                     /gene="walK"
                     /locus_tag="NBH55_001819"
     CDS             7584..9419
                     /gene="walK"
                     /locus_tag="NBH55_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCD1509801.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            9416..10732
                     /locus_tag="NBH55_001820"
     CDS             9416..10732
                     /locus_tag="NBH55_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509802.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            10733..11602
                     /locus_tag="NBH55_001821"
     CDS             10733..11602
                     /locus_tag="NBH55_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCD1509803.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            11657..12466
                     /locus_tag="NBH55_001822"
     CDS             11657..12466
                     /locus_tag="NBH55_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1509804.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(12569..13198)
                     /locus_tag="NBH55_001823"
     CDS             complement(12569..13198)
                     /locus_tag="NBH55_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCD1509805.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            13368..14660
                     /locus_tag="NBH55_001824"
     CDS             13368..14660
                     /locus_tag="NBH55_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1509806.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(14736..15398)
                     /locus_tag="NBH55_001825"
     CDS             complement(14736..15398)
                     /locus_tag="NBH55_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1509807.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            15623..15907
                     /gene="groES"
                     /locus_tag="NBH55_001826"
     CDS             15623..15907
                     /gene="groES"
                     /locus_tag="NBH55_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCD1509808.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            15958..17583
                     /gene="groL"
                     /locus_tag="NBH55_001827"
     CDS             15958..17583
                     /gene="groL"
                     /locus_tag="NBH55_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCD1509809.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRSAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6150 a         2866 c         3662 g         5114 t
ORIGIN      
        1 tcttcctaaa aaattattag aagaaagatt agttcttcgt tttggatctg caacacatga
       61 agcaaaagta tttttgaatg gaaaattgat taatcaacac aaaggagggt ttacgccatt
      121 tgaatcagaa attaatcagt ttataaaaga tggaagtaac agaattacag tggctgtaaa
      181 caatgttatt gacgatagca ctttacctgt agggattttg aaagaaacga tacaggaaga
      241 tggagacata aaaaaagaaa atttggttaa ttttgacttt ttcaattatg caggcattaa
      301 tagacccgtt aaattgtata caactccgaa agactatata aaggatgtca ctattaatac
      361 aaaaataatt gattcagatg caaatgtaga atttaatatc gatacagaat ggaccggaga
      421 aattctaata gcaatctatg acgaagaagc tcaacttata gcagaagcta aggggaaaga
      481 aggagtgatt caacttaaga acccaacact ttgggagcct ctaaatgctt acttatatag
      541 tatgaaagtt agtttaatta acgaaaataa agtgattgat acgtacacag aagaatttgg
      601 cattcgtagt attcaggttt cgaatgggaa gtttttgatt aataataaac ctttttactt
      661 taagggattt gggaagcatg aggacacgta tattaatggt cgtggtttga atgaagcagt
      721 aaatatcaaa gattttaatt taatgaaatg gatcggtgct aattcattta ggacttcaca
      781 ttatccttat tcagaagaaa ttatgcgctt agcagatcga gaaggtattg tagttattga
      841 tgaagtacct gcagtaggat tacatttgaa tttcatggct actttattag atgacaacgt
      901 agaaaaacat gatacttgga aagaaatacg gacagaaaat gctcacaaaa tggcgattaa
      961 ggaattgata gaacgcgaca agaaccatcc gagtgtcgta atgtggtcta ttgcaaatga
     1021 accagattcg gattctgaag gcgctaaaga atattttgaa cctttagttg aattgactaa
     1081 agaacttgac cctcagaaga gacctgtaac aatcgttact tacttaaagt caacaccaga
     1141 tatttgtaaa gtaggagata ttgtcgatgt tttatgttta aatcgctatt atggttggta
     1201 tgtttcaggt ggagacctaa aacatgcaaa atatctgttg aaaaaagagc tagatggatg
     1261 gatggaaaga tgtcccgata cgccaataat gtttacagaa tatggagcag atacagtagc
     1321 tggacttcat gatacagtac caacaatgtt tacagaagag tatcaagtgg gttattacga
     1381 agcaaatcac gaagtaatag ataaatgtga caattttgtt ggtgaacaag tgtggaattt
     1441 tgcagatttt gaaacaagtc aagggatttt aagagtgcaa ggaaataaga aaggaatctt
     1501 tactagagat agaaagccta aaatgattgc tcactatcta aagaaacgat ggtcatcaat
     1561 accaaatttc agttataaaa aataatttac cttttataag gaatataaaa aaggagtggc
     1621 tgcttaattg ccaactcctt ttttttataa aaagattttc tgtttaaaga aggatatttt
     1681 tttctagttt tttcgaaaaa aaggcgcttt ctgattgttg tattagtgga aaggggaaaa
     1741 aaggagcata tttatcatgc aacaaaatta caatgagaaa tctatacaac atcaatttga
     1801 tgcctactgt aaaaaagtat tacgaaacga aactaagtct attcgaaaac gaaacattaa
     1861 cataacagat aatgaggaac cattgacgaa ttcaaatgaa ggtaagtact cccaaactca
     1921 ttttgatgaa caagatgtct atttcttatt tggaatggaa atattaattt cagatcaaaa
     1981 gttatcggca gccattgatc atttatcgga aataagaaga aaggttatcc tactttatta
     2041 ttttgcaggt tttaatgata ctgaaatcgg taaaatcctc aatatgagta caagtggtat
     2101 ctggtatcaa cgaaagaagg cagttgaaca attaaaaatg gggtatgact tatggtagac
     2161 aaccccaaaa aacggcagta tccattagtt cctctatcca ttatccaatc tgctctagaa
     2221 gaagatccaa tttcaatcaa gatgatactc aaacattatc atagttatat agctaagtta
     2281 tgtttgacaa atggatttaa tgaggcgggg caatttatta cctatgtaga tgagtatatg
     2341 ctaagacagc tagaaattaa attgatagaa gcgatcttaa aatttaaaat taactaacta
     2401 aaaagtcccc tttccactta ttctgtttaa aagaatcttc taatagttaa aggattagta
     2461 gattctttta aacagaaaac ctattgatca ttgaaattta aatacattct atcagtacgt
     2521 taacacttca aggagcatat tttagttgcg ccatgatttc ttactgtaaa gaaggagcga
     2581 taaacattct aaaataattt ttatagtcaa taaaaagcga tgatttgaag ttgggataat
     2641 gatacttccg taaggctcgc cgtaaacttg gagagaggtg aaattcctat gtgcttttga
     2701 caaaagcagc tgattaagaa ttatttccat atgttgattt caacattttt tctattattt
     2761 ttaagaccaa attcagtgag gcaaatagca tcatgtaaat ttagtttttc tattgaacgt
     2821 tctccacttt ttagtttttt tatagtactc aaatttaatc ctgtatgttt agagatctca
     2881 tttgcagtat ggttttgtaa agtttctcgg attattgtta tattagcaat ttcattacgc
     2941 ataattatgt tcctttctta tcaaaaatat caatgactac agtgggcttt tagtgaaaaa
     3001 aaggggtact acagtctctt ttgaaaacaa aaaaatacag tatatacgtt ttctaaatgc
     3061 taagcaccaa cgtttacact gtatttaagg gtttggcacc caatgggctg tgaggggctc
     3121 gaacccgcga cccgatgatt aagagtcatc tgctctacca actgagctaa cagcccaaat
     3181 taatactttg ttattctaac acctaaaata aaaaagtgca atacttatta tcagaattga
     3241 gagtgaattt tatggatttt caagattatg aaaaaatatt tgaaagttat ccatcgatat
     3301 tgaaaattac tgatttaact aaaatgttaa atattggaaa aaataaagct tataatttgc
     3361 tgaaagatag atcaatagaa aatataaaaa ttggagcaag gtacaaaata cctaaaaaag
     3421 ctgtcattga gtatatattg aaaatacaag atgaaggtaa aaatcatgct ataataaaat
     3481 agtcaaaatt tttgccgata tttcagttta agaggagaga ttgaattgaa attaagagca
     3541 aacataattg aaaaaagagg aacattacaa gtagttgtat cttataaaga ctctaacgga
     3601 aaaattagac aaaagtggaa agatacaaaa ttaaaaaaga aaggaaataa aaaacgagca
     3661 gaaaaaatta gagatgaatt tttactagaa ttggaaagtc aattagctat ggaatgtgaa
     3721 tgtgctaatc caaatttatt gttttatgac tatttgctta attatttgga agtatcaaaa
     3781 aaacaagtag catttaacac atatgttggg tataagcatt acgtccataa tcgaatttac
     3841 aagttttttt atcctaaaaa aattaaacta aataggttaa agccttatca tttacaagag
     3901 ttttatcaat atatgttaaa tgataactgt acagcaaata cggttattca ttatcacgca
     3961 tttatacgca aagctcttca agaggctgtt attacagaat tgataagtac aaatgtagca
     4021 gataaagtaa gaaagccaaa aaagaaacaa tttgtttctc aagtttataa tcaacaagag
     4081 atcttgaaat tacttgacat tttgtcagaa gaaaaactct atttggtagt tcttttaaca
     4141 gcattttatg gattgcgaag aagtgaagtt ttaggattaa aatggtctgc tattgatttt
     4201 ttaaacaaaa aaatcatgat aaaccatgta atcatagaaa atccagagaa tatgtcacag
     4261 ttaataaaga aagatcaaac aaagaactct tccagctatc gtacattacc tttggttgaa
     4321 acgattgaac gagcattagt aaatcaagct aaatggcaac aagataatcg tattctttta
     4381 ggagatgatt atattttaga agataatgag tacgtattta ctatggaaga tggacggttg
     4441 atgaagcctc aatatgtaac gcatcgtttg agtaagttga ttaagaaaaa tggattgaaa
     4501 aaaattcgct ttcatgactt gcgtcattct aatgcttcaa taatgttaga tagtggccag
     4561 aatatgaaat ccattcaaga atggctggga catgcatcgt attcgacaac tgctaattta
     4621 tacacgcatt taaccgcagg agtaaaagat cgtgcagcag aagcattaga aaacgcattt
     4681 gggtttaaag aataaaaaaa caaaaaacaa tatctttggg gtatcgcttc ttgtttttag
     4741 aatttttttg ttggcaattt gttggcagta tgttggcaat gaaaaaattc acaaactaaa
     4801 aacctattta taaagcttta taaaactttt ttcacaaata gagcatttgg ttaagagtca
     4861 gctgctctac caactgagct aacggcccgt acaaaaagta cttaataata gtagcactaa
     4921 gaattttaaa atgcaagact acatttaaag aaaatcagaa aaatctttac ttccttatag
     4981 gaaatcattc atagattatt cacatttgct cgctattata taagcatacc aacaaacaaa
     5041 gttcttgcaa gagaacaaac cctaacactt ttttcatttt ttactccttt cccgccgtct
     5101 ccccggcggg tctcctgttc ttaacgataa aagacaagaa ggcttagcct tcttgttttt
     5161 tgttttcatg gaatcccgct aatcaaatca cctagttaaa gcaactagat tgaatgttta
     5221 tcataagtct gatatcatgt ttatgaaatg aaaaaaggag cggttatgat ggaaaaaatg
     5281 cgtcagcaat taatggagtg ggggaaggaa ctagaaacat tccgtctgcc tcactgggaa
     5341 gaactccctg acttagaact ttatatggac caagtcagaa cgttagttga tcgttatctt
     5401 tcacctgtga tacaaggaga aaagcatcca ttgcttacct cttctatggt aaacaattac
     5461 gttaaactag gtttgatacc agcaccagtt aaaaaaagat ataacaaaga acacgtagca
     5521 tttttacttg caatcactac gttgaaacaa gtattgacta ttcctgaaat caaagaaggg
     5581 atattgttcc aaggaaaaac agtaggaatt cgtgaagcct ataatctatt ttgcgatgaa
     5641 caagaagcag ctgtgtggat ggtcagccaa ttagcacaag gtaaatctca tccgcaaaaa
     5701 ttcgaccagg caacccctgt agaatatatt gctgtaagag cggcaacttt atcgtttgca
     5761 atgaaacttc ttgcggaaaa aacgattgtt ctagaaaccg aatatttaaa agaggagaaa
     5821 acaaatgaaa aacaataaaa tcgcaatttt agtagattca ggtacggatg tgccagaaga
     5881 attgataaaa aaatacgaga tgtatatgat cccattgaaa attatttata aagatcaggt
     5941 atatacagat aaaattgaca ttacctctga agagatctat caacgtttgc cagaggaaat
     6001 ccctggtaca tctttgccgg atggtgagtc gatcacagaa atctttgagc aaatcaaaaa
     6061 agacggttat gaaaaggtac ttgcggtaac aatttccagt ggattgagcg ggacatataa
     6121 tgtggttcgt ttaattgcac aacagcaaga agaattggaa gcatatgtac tagatacgaa
     6181 aaatattggt attggtgcag gtttttctgc gatccaagca gcaaaatggc tggaagaagg
     6241 agtagaatgg gatcaactga tcagtaatct gaatgagtta gtcgaaagaa caaaagtttt
     6301 ctttaatgtt gcgacattgg aatatcttca aaaaggtgga cggatcggtt tagttgcttc
     6361 tattgtagga accgctttaa agcttaaccc aatcatctca tgtaatgaag aagggattta
     6421 ttacacagta ggaaaagcta ggggacgtaa aaaaagtttg gataaaacgg ttgcttacgt
     6481 caaagaaaaa atcggtaatg caaaggtctt caacttagct gttgcccatg gggatgcgaa
     6541 agaagaagca attgaaatgg aagcgagact taaaagagag ttcccacaag caaaccaaat
     6601 ctactttggc cagatttctc cggcattagt agtccacact ggtccgggat tattgggtgt
     6661 cggtgtacaa gttttagaag gataaatcaa aaaaaggaaa cactgaggtg aaaatcagtg
     6721 ttttcctttt ttattgaagt tagaacaaaa aataataaag tattttgcac tttcatttta
     6781 attttctctt ttgttttata ggcaaagatg aaacaaaagc agaaatacgg tataatgaga
     6841 aggtatgaca ataatatata agaggtggct ctttatgaaa aaagttttag ttgtcgatga
     6901 tgagaagccg atttcggata tcgtcaaatt caatttagcc aaagaaggct atgatgttta
     6961 tactgcatat gatggggaag aagccttgga aaaagttgct gaggtagaac ctgatttgat
     7021 tttacttgat ttgatgctac ctaaaatgga cggcttagaa gttgctcgtg aggtacgcaa
     7081 aacttatgat atgccgatca ttatggtaac agccaaagat tctgaaattg ataaagtcct
     7141 aggcttagag ttaggcgccg atgattacgt gaccaagcca ttttccaacc gagaattagt
     7201 tgcacgtgtc aaagcaaatc ttcgtcgcgg tgccacggct gcgaaggaac cagaagaggc
     7261 agcacctgcg gaattgacga ttggtgattt gacgatccat ccagaagctt acatggtgac
     7321 gaaacgagga gaaacaatcg agttaacaca tcgtgaattc gaactactct tttacttggc
     7381 gaagcacctt ggacaagtga tgacaagaga acatttatta caaacggttt ggggttatga
     7441 ttattttgga gatgtacgaa cagtggatgt aactgtacgt cgtttgcgtg aaaagatcga
     7501 agataatcca agccatccaa attatttagt gacacgtcgt ggtgtcggtt actatttgcg
     7561 taacccagaa caggagtaat tggatgaaaa gaaaagtccg cttttttcga tcagtcaatt
     7621 ttaagatcgc tattaccttt attctgattt tattgatctc gatcgaaatt attggtgcct
     7681 actttatacg tggtttagaa cgttccacga tcaatacatt tattaaagat atgaatcaaa
     7741 cggtggaatc attagcaaca acaatcagcc cggaattgaa tcgaaaagat aatgcagatg
     7801 atgaagaggt aaatgcgaat atcaaaagat ttattgaaaa cagtgcgact agcgatatca
     7861 tcgaaatacg ggtcgttgac gaaaaaggga tcatccgagg aacaaccgat gtcaatgaac
     7921 aaagcgctgt gggaaagaaa aatgattatg ttgatatcaa tgatttcacg actaaacgtt
     7981 atgttgcttt agataacgac gataaacggg tgaatatcaa tgttcaaccg atcctgtccc
     8041 cgaccggcga tgccgttatc ggggctcttt acgtcaaaag taatatcgaa caaaaatatt
     8101 cggaaatcaa taacacagca gtcattttct ttactgcctc actaattgcg gcatttattt
     8161 caatgatcgt atctgtttta gttgctcgtt cgattacaca gccgatcggt gaaatgagag
     8221 aacaagccat tcgaatcgct agaggagact acagtcggaa agtcaaagtg catggacaag
     8281 atgaacttgg acagctggct gataccttca atcagttagc tgaacgtatt gaagaagctc
     8341 aagatacaat ggaagctgaa cgcaatcgtt tggatagcgt gctatcccat atgacagacg
     8401 gcgtcattgc gacagatcgc aggggtaaag tcattacgat caatgatatg gctgtttcct
     8461 tgctagatat caaaaacgag gaagcaatcg gacaatcgat tttgacactt ttagatatcg
     8521 aagaagaata tacgttgcgg aaattattag aagatccaaa cgaaatggta cttgaccgtt
     8581 caaccagtac attggaaagt gatcagatga tcttgcgagt agacttttct atgatccgtc
     8641 gcgaatcagg ttttatcagc ggattagtag ctgtcttgca tgatgtgaca gaacaagaaa
     8701 aaacagaacg ggaacgtcgt gaattcgtct ctaatgtttc ccatgaactt cgtacaccgt
     8761 tgacaagtat gcgtagttat atcgaagcat tgagcgaagg tgcgtggaaa gacgaggaag
     8821 tagcaccaaa ttttttgaag gtaacgctag atgaaacaga ccgaatgatt cgaatgatca
     8881 atgacctctt aaatctatct cgcatggata caggtaatac ccagcttcaa ttagaatatg
     8941 tcaatttcaa cgagttagtg aattttgttc ttgaccggtt tgatatgatg gtgggaaatc
     9001 aggaaaaaaa ttataagatc cgtcgtgaat ttacgcaaag agatctttgg gtcgaagtag
     9061 atacagataa aatcatccaa gtagtcgata atatcatgaa taacgcgatc aagtattcac
     9121 cagatggggg aacgattact tgtcgcttat tagaaacgca taataacgtg gttttaagta
     9181 tcaccgatca aggattgggg attcctaaaa aagatctgaa ccgagtgttc gagcggttct
     9241 atcgagtaga taaagcacgt gcaagagcac aaggtgggac aggacttgga ttagctattt
     9301 ctagagaagt gattaaggcg catcgaggcg ctatttgggc agagagcaag gaaggaaaag
     9361 ggtcgacttt ttatatctcg ctgccatatg aaccttatga ggaggaatgg tgggaatgag
     9421 aatctctgaa aaagtcgttc gtataggact cattttgatg gttgcgttaa gcatatactt
     9481 ctcttatgca atctggctga gcccagcagg aaaaagttca atcaacgtag atgagacaaa
     9541 cactcaaatg atcgagtctc aaagcaatcg aaaaacttcc gaagtctttc ttccgcttca
     9601 tgttacatgg caccattcgg gaatgatcca agaaaccaac agtgaaaatc tcgtgtaccg
     9661 attgcaattg atcatcgata aagcaagatt tggaaagctt tctgaagtag tttcaggaga
     9721 caaatcacag tttgaacagg tgaaaaatct gaactctggt tttgagttgg cctataatgc
     9781 gccttttttg ctaagagaat acaaggaagc atttgattta gatttagatg tgtcgaactt
     9841 ggcaaaagaa agcgaaaata tgtattttac tagaatacaa ttcgatcaag gaaataaaaa
     9901 aatccgattt ttgaattatt cgaaccatct cgtatatgaa gcaaatattt cgttgaattg
     9961 gacagatatc aataaagaat taacagcaaa agacacaaaa tggacagaaa tgacaacaga
    10021 accatcgacg atcgacacgc aatatgctac gaaacattcg atcaaactga aaaagtatag
    10081 ttatatcctg tcacagcaac cctatacact ttttagaaat gcttttttcc agaaaccaga
    10141 tagcgtgaaa aataatgatg aaagcaatga attgattttt tacggtggga atgaaacaat
    10201 gtctatccat tcggaaacgc aagtagttga ttttcgtgga gagctgccag agaaaaaaga
    10261 aaatgctagt gtattttctg aaagtttccc ctatattagc aaattaggca gttctttagg
    10321 gaacctcaga tattttgacc gcagtgacaa tacgatcgat tatcggatat tcgtggaagg
    10381 cttcccggtt tttgggacag acagcagagg acagattggt gtggaagttg gcggtgaaac
    10441 gaatggttct gtgcaagtga atatccaaac cagtttgaat acgattcaag tgccgatacc
    10501 ttcagacgaa gaagtagaac ttgcaagtac gaatgaaatc atccagcagt taaaagctaa
    10561 gggagcagat agcaacaaga ttggttcagt gatcgttggc tatacatggc aaaatgtcaa
    10621 agaaacgaat cgtgtcgttg atctcttacc tgaatggtat gtgaaatata aagataactg
    10681 gtattcagct aacgaccttc tggaaaaatt acctggattg gaggcgaatt aattggattt
    10741 caaaaaaatc gaatggatat ttttcgtcgc ttttttaggg ttgaatgtgt ttttattcag
    10801 cagttatcat gacgcggtat accaagaaaa caatgtcatt cgctctaatc agactgaatc
    10861 aatccaacaa cgtctggcta gcgacgatat cacttacacg gggaagattt cgtctgagaa
    10921 caagcaaggc tattatctta gcgcggagca gaccaatctt agcacttcat tagcgaatta
    10981 tcgcaaggct ggtaaaagcg gcttgctggc aagtggtgtg tttttagacg gtgatgtttt
    11041 gacgaaatct ttatccagtt cagatgctga aaagaacgtg atcgatccag ataaaattag
    11101 tagttccttg gaagattttt tatctaacaa agacaatatt ttattcggta atgattatgt
    11161 gtacacgaaa aacttttctc atttagaaga agaaccacta gaaatccaag caagccaaga
    11221 gtatgaaggc atcccattca atgatgatac agcaaaaatc gttctttctt tagaaaaaaa
    11281 cgataatgat tatagcatca cgaaatacat gcagacgcat ttatctgata tcgagcagct
    11341 gcgcgaaaag acggaactgt atacggaaga ggaagcaatc aatacattgt acatcaataa
    11401 caaaatacca agaggatcaa aaattttatg gcgtcagcta gcttattcct gtattctgaa
    11461 agtccgggaa aaaaatgtgt atgttcctgt ttggcatgta gcgatcgaaa caagcgataa
    11521 agtggtccaa attgagagtg tcaacgcttt cagcaatacg atcgtgacaa acaatactgt
    11581 accaaaagtg gaagatcaat aaaaaagcga gtataatgaa aaaagtttac aaagtaagaa
    11641 gggaagtagg agtcaaatgg ctgaagaaaa accagctttt aaaatcagta tcctagcaag
    11701 tggaagttcg ggtaattcac tttatatcga aagtgaaaaa aaacggcttt tggtcgatgc
    11761 aggacttagc ggtaaaaaaa tcacttcctt gatggctcaa attgatcgga caccggacca
    11821 attagatgga atacttgtga cgcatgaaca tcgagatcat atacatggcg tgggtgtctt
    11881 ggctcgtaag tatcatttgg atgtttttgc caatcagcag acttgggaag cgatggaacc
    11941 actgattgga aatgtaccag tggaacaaaa acatctattt gagatgggga aagtcagaac
    12001 attcggcgat atcgatattg agagtttcgg cgtctcccat gacgcagcgg ctccgcaatt
    12061 ttatcgtttc cataaagaaa atcggtcatt cgtcatgttg acggacaccg gatattgcag
    12121 tgattatatg cgaggtgtca ttgaaaatgc cgatggttat ttgatggaaa gtaatcatga
    12181 tcttgagatg ttacgcatgg gaccttatcc gtggaattta aaacagcgta ttttaggaga
    12241 tagaggacat ctttccaacg aagacggtgc acttgtcatg actgatgtga tcggtaatcg
    12301 gaccaaacga atctatcttg gccatcttag caaggaaaac aatatgaaag aacttgctca
    12361 tttaacaatg gaaaatgttt tgaaagaaaa agattttggc gttggacatg attttgaaat
    12421 ctatgataca gatccagatg aagcaactga gctattttcg atttaaacag ttcaatcaat
    12481 aaaaatataa aaagcagaag ataaaatcaa tcagggttgg ttttatcttc tatttttatt
    12541 tttttagcat attaaaaaaa taaccgtatt atcgactgac tacactgatg tgctgctttt
    12601 catgtttctc atccagtatc tcatctacta atgcaatcgc gtaatctgcc atgctgatcg
    12661 tactgtcgcc agcagcgttt ctttgtaaat aatcatcact taacaaataa gtacctgtac
    12721 gtttgccatg tggatcaaat aaagaagctg gactgatata agtccaatgt agatttttag
    12781 attgtttcaa ttttaacaat ccttcgtaca tattgaaagc tgttggatag aagggtgcat
    12841 ttttaggtac ttggtcaatc atacgagtcg tcttttctgt atctaagaat aacgaagagg
    12901 cacccccaac gacaattaag cgtgtatcgg ttccttcaag catctcaatc aaatgggaca
    12961 aagaagtttg atgcatttct tcttttcctt tgggagcgtt gaaagcatca acgacaacat
    13021 caaaagatgc aagatcagta gttgtcaact caaataaatc cttttgtata aacggaacat
    13081 ctgatggcaa tttttctgga tgacgcacga ttgccgtcac gtctaaatct ctggacaatg
    13141 cttcgtctaa aataaatgaa cctgcatggc cagtagctcc aatgagtgca attttcataa
    13201 aaaacctcct tttttattcc tgtccattgt aagcagttgc tgaatattcg tcaaagaatc
    13261 cgatttttga aaagctcagt gcgcattcct ttcatggttc gtgatcattg ggtatagtta
    13321 aattaagaaa actgattgat aaaaatagaa gttgaaggag ataagaaatg aaagagtggt
    13381 tagatcgtgc ctttttacca atgataacat ctgttgtgaa tcttagaccc ttaactgctt
    13441 tgcgtaatgg aatgacttac acgatcccaa taattattgt tggctcgctg tttttcttat
    13501 ttgctcattt tcctgtggat tacatcgctc agtggataga caaaacagga ttttctatgt
    13561 attttacaca agtataccag gctacatttg atgtgatagc cttttggatc gtttttgcca
    13621 ttgcatacgc ttatgcaaaa gaagaagaca tcgatcgctt accagtcggc ataacagcct
    13681 tgtctgctta tctactgctt attcagccac taaatactca gatagatact gtctggacag
    13741 gtgctgaagg gatactagga gcagttacaa ctgggctcat catcggatgg ggctatgcac
    13801 gtttagtaag acttgcgaaa gggtggaagc aaagagagaa gatacctgaa aatgtttggc
    13861 agtcttttac ttcattgatt ccaatcattg ttatagtcac ttttacgctt atcggttcag
    13921 cggtttttca acagttgttc agaatgactg ttgttcaagg agtatggaca tatgtccagc
    13981 ttcccattca atcgttcata gacacgttgt ttggtgtgct tttattaggt ttttttgttc
    14041 cgtttttatg gttatttggt cttcatggat ctgcgatggt caatgggttg gttagtcccg
    14101 tcttgcaagc gaattcatta gcgaatgcag aaatcttagc ctcaggacaa gagttgacgg
    14161 tggcaaatgg cggacatatc gttactcagc aatttctcga tcagttcatg acggttacgg
    14221 gtgctggatt gacgttaggg gctgtatttt ttatgacggt ttttgcgaaa tcgaagaaat
    14281 acagagaatt aggaaatctt gctcttttac cagccttttt caacatcaat gaatcaatta
    14341 ttttttccac accgatcgtg atgaacccga tgatggctgt tccttttatt ttcgcaccgg
    14401 tcgtttcagg attgattacc tattccgctt tatattttgg attcgttcct ttatttacag
    14461 cagtccaagt cccttggaca acaccgccga tcctctcggg tttcttagta ggcggagtgc
    14521 ctgcagcgat tttacaaggt atcgtgttgt caattagttt tttcatttat ttcccatttt
    14581 tgaaaaaaat agattctgca aactgcaaaa aggaaagaca gacaaaaaat gatggagcag
    14641 cagaaaagat gaacgactaa aaaacaaaaa tactcctgtc gctttagatt gggaaaatca
    14701 ccaatctagg atgacaggag tatttatagt catgtttata ctagcccttt ttggacagct
    14761 aattgaacaa gaacaactaa tgtgttcatt cctacgtgtg tgatcatcga tgtccagatc
    14821 cgaccagttg ctttgtactc taggctgaag aagaaaccta ggaaaaagta gattagcaga
    14881 tgaccatcat tatgagcaaa cgcaaacagc aaagagctga tcactgctcc gacccaaaaa
    14941 tttgaatatc gactgatgat ccccagtata ctgcggcgga atacgaactc ttccatgatg
    15001 ggtccagcaa cagttgtcgc caaaataaaa gcgggtgctt ccatgatcat ctggatgata
    15061 ttttgtgtat tctcggaagg ggttgctttg ccaaacaata cagattctaa catgacagcg
    15121 atgctttgca gaatcaatgc gacaaagatt ccaaccaatc cataaataac aatctttgga
    15181 gcagaagctt tttttaccgc tgattcgatc gctaagggtt cacgttgctt aaaataaaaa
    15241 atagctagga caacggcgcc tagtatatac atgactgcag ttgctgtgat caattcagac
    15301 gtagatttta caagtcctat cgaagctagg atcaagggtg agataaaaat tagaccatag
    15361 caaaaaatgc tgaggaagct atattttttt aaagacatgt aattcctcct tttaagggat
    15421 aacaagcatg caaggcaagc ttatatcgat tgatttcaat tatatcataa tttcaataag
    15481 cagctgtcag aataaaattt cttttctttg atttaaaagc ttgcaaaaaa gagggagaat
    15541 gagtattatt aaaaatgtag gttagcactc gaaggttaag agtgctaaca gattaaaaaa
    15601 agagttatcg gagggattta tcgtgttaaa accattaggt gatcgcgtca ttatcgaagt
    15661 cgcaaaagaa gaagaaaaaa ctgtcggagg aatcgtttta gcttctgctg ctaaagagaa
    15721 accccaaact gggacagttg ttgcagtcgg tgaaggccgt ttgttagaaa acggtgaaaa
    15781 agttcctgcc gctgtaaaag caggcgacca agttatgttc gaaaaatacg ccggtacaga
    15841 agtaaaatac gaaggaaaag aatatctgat cgtcgcagga aaagatatca tggcgattgt
    15901 tgagtaaatt tggaagataa aaaaataatc aaaaataatc aatgaggtga atcaatcatg
    15961 gcaaaagaat tgaaatttgc agaagacgca cgcgcagcta tgttacgcgg ggtagataaa
    16021 ttagcagata cagtgaaagt aacattagga cctaaaggcc gaaatgtcgt actagaaaaa
    16081 tcttacggct ctccattgat cacaaatgat ggtgtgacga tcgcaaaaga aatcgaatta
    16141 gaagatcatt ttgaaaacat gggtgcaaaa cttgtatcag aagtagcatc aaaaacaaat
    16201 gatattgcag gagacggtac aacgacagca actgttctga cacaagcaat cgttcgcgaa
    16261 ggtttaaaaa acgtaactgc aggtgctaat cctctaggca ttcgtcgcgg gatcgaattg
    16321 gcaacaaaag cagcagtcga agaattgcat aatatctcaa ctgttgttga ttcaaaagaa
    16381 gccattgcac aagtagcagc tgtttcttcg ggttctgata aagttggaca tttgattgct
    16441 gatgcaatgg aaaaagttgg aaacgacggt gtcatcacaa tcgaagaatc aaaagggatc
    16501 gaaacagaac tagatgttgt agaaggtatg cagtttgatc gtggttactt atcacaatat
    16561 atggtaacag ataacgacaa aatggaagct gttctagaaa acccatatat cttgatcaca
    16621 gacaaaaaaa tctctaacat ccaagatatt ctgccattgt tagaacaaat cttgcaacaa
    16681 tcacgtccat tgttgattat tgctgacgat gtagatggtg aagcattgcc aactcttgta
    16741 ttgaacaaaa tccgtggaac attcaacgta gtagctgtaa aagctcctgg tttcggcgat
    16801 cgtcgtaaag caatgcttga agatatcgct attttgacag gtggtacagt aattactgac
    16861 gatctaggtt tagaattgaa agatgtaaca atcgaaaact taggaaatgc ttctaaagtt
    16921 gttgtagata aagacaatac aacgatcgta gaaggttctg gagaaaaaga agcaatcgaa
    16981 gctcgtgtgc aattgatcaa aaaccaaatt gctgaaacaa cttctgattt tgaccgcgaa
    17041 aaattacaag aacgcttagc taaattagca ggtggtgtag ctgttgttaa agttggtgca
    17101 gctactgaaa cagaattgaa agaattaaaa ttacgcattg aagatgcatt gaatgctaca
    17161 cgttccgcag tagaagaagg aatggtctca ggtggtggta cagctctagt gaacgttatc
    17221 agcaaagttt ctgctgtaga agcagaagga gacgtggcaa caggtatcaa gatcgttgtt
    17281 cgtgctttag aagaaccaat tcgtcaaatc gctgaaaacg ctggttatga aggatcagtg
    17341 attgttgata aattgaagaa cgtggaacta ggcactggtt tcaatgctgc tactggagaa
    17401 tgggtaaaca tggttgaagc aggtatcgta gacccaacta aagtaacacg ttctgccttg
    17461 caaaatgccg cttccgtttc tgccttgtta ttaacgacag aagcagtcgt tgcagataaa
    17521 ccagaaccag cagcgccagc agctcctgca atggatccat caatgatggg cggtatgatg
    17581 taagaaaaag caacttgctt atctacaact gatcattagt gaaagacaga taaaaagagg
    17641 ctggaacaga agcgtttaac tccaagaaat aagaagaaat tcacggaaat tgcttttcaa
    17701 atttttgtga atttcagctt atttccgaag gagttgcttc tgcttccgct gtttatacgt
    17761 gtttagagcg tgagacaaaa gtgttcttta ct
//
LOCUS       DAHHPA010000059        17290 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.060, whole genome shotgun sequence.
ACCESSION   DAHHPA010000059 DAHHPA010000000
VERSION     DAHHPA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.060"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..535)
                     /locus_tag="NBH55_001828"
     CDS             complement(<1..535)
                     /locus_tag="NBH55_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1509810.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGA"
     gene            complement(547..1239)
                     /locus_tag="NBH55_001829"
     CDS             complement(547..1239)
                     /locus_tag="NBH55_001829"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCD1509811.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1258..1542)
                     /locus_tag="NBH55_001830"
     CDS             complement(1258..1542)
                     /locus_tag="NBH55_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509812.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1555..2118)
                     /locus_tag="NBH55_001831"
     CDS             complement(1555..2118)
                     /locus_tag="NBH55_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCD1509813.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2346..3008
                     /locus_tag="NBH55_001832"
     CDS             2346..3008
                     /locus_tag="NBH55_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCD1509814.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDPRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3132..4328
                     /locus_tag="NBH55_001833"
     CDS             3132..4328
                     /locus_tag="NBH55_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCD1509815.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4429..5805)
                     /locus_tag="NBH55_001834"
     CDS             complement(4429..5805)
                     /locus_tag="NBH55_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1509816.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5842..6708)
                     /locus_tag="NBH55_001835"
     CDS             complement(5842..6708)
                     /locus_tag="NBH55_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCD1509817.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6797..7822)
                     /locus_tag="NBH55_001836"
     CDS             complement(6797..7822)
                     /locus_tag="NBH55_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1509818.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7840..8514)
                     /locus_tag="NBH55_001837"
     CDS             complement(7840..8514)
                     /locus_tag="NBH55_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1509819.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8698..9591)
                     /locus_tag="NBH55_001838"
     CDS             complement(8698..9591)
                     /locus_tag="NBH55_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCD1509820.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9665..11362)
                     /locus_tag="NBH55_001839"
     CDS             complement(9665..11362)
                     /locus_tag="NBH55_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCD1509821.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11355..12668)
                     /locus_tag="NBH55_001840"
     CDS             complement(11355..12668)
                     /locus_tag="NBH55_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCD1509822.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12668..17176)
                     /locus_tag="NBH55_001841"
     CDS             complement(12668..17176)
                     /locus_tag="NBH55_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCD1509823.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEGELPLQVVV
                     DERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSGQALQRIQRQLFNGILER
                     LGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDG
                     ENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYN
                     GAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASM
                     SELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSD
                     IFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVV
                     GDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFT
                     HPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAI
                     NTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTP
                     RYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPG
                     ENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQV
                     KLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQ
                     NGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWI
                     REIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYY
                     EGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFEL
                     YETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPD
                     SFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENW
                     QYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLY
                     RAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNG
                     YRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLEL
                     LKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKE
                     VKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQ
                     QELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVR
                     QSKKEGAR"
BASE COUNT         4844 a         3894 c         3008 g         5544 t
ORIGIN      
        1 ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac
       61 ttgccacggc atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg
      121 tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt
      181 ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc
      241 aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt
      301 cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg
      361 aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact
      421 tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct
      481 ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag
      541 tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc
      601 gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc
      661 acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc
      721 catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga
      781 gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt
      841 tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc
      901 tggtaattgt cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt
      961 gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt
     1021 attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa
     1081 gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat
     1141 aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt
     1201 tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta
     1261 tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt
     1321 catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat
     1381 tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg
     1441 ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg
     1501 cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt
     1561 tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa
     1621 ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc
     1681 gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg
     1741 aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt
     1801 cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc
     1861 ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg
     1921 aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc
     1981 aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac
     2041 agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa
     2101 ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc
     2161 atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc
     2221 tctaaatata gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa
     2281 taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt
     2341 ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg
     2401 tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt
     2461 tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga
     2521 ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt
     2581 ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc
     2641 agacagccaa acttaaagca atcgatcccc gccatgatcc agtcaaagca gcgaaagctt
     2701 tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata
     2761 cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga
     2821 tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt
     2881 cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg
     2941 tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa
     3001 aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt
     3061 tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg
     3121 aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata
     3181 cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg accacttcac
     3241 aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac
     3301 taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg
     3361 cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat
     3421 cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac
     3481 tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct
     3541 tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa
     3601 agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc
     3661 taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac
     3721 tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc
     3781 cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg
     3841 attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac
     3901 agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg
     3961 ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga
     4021 tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa
     4081 caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag
     4141 cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt
     4201 tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc
     4261 atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa
     4321 cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac
     4381 tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt
     4441 catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat
     4501 accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat
     4561 ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc
     4621 aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat
     4681 gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc
     4741 cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat
     4801 aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat
     4861 catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac
     4921 tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt
     4981 atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag
     5041 tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc
     5101 cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac
     5161 accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc
     5221 tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg
     5281 taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc
     5341 aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag
     5401 ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga
     5461 ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc
     5521 accaaataca acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa
     5581 ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga
     5641 cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc
     5701 gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc
     5761 actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca
     5821 ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa
     5881 aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc
     5941 aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc
     6001 aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa
     6061 taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa
     6121 gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa
     6181 attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc
     6241 tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc
     6301 ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac
     6361 ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat
     6421 taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg
     6481 gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat
     6541 ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc
     6601 gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc
     6661 aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct
     6721 tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt
     6781 cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag
     6841 tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg
     6901 ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat
     6961 ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc
     7021 ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt
     7081 ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga
     7141 tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt
     7201 tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat
     7261 attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt
     7321 ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa
     7381 tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat
     7441 ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta
     7501 cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg
     7561 tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc
     7621 cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct
     7681 caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca
     7741 tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt
     7801 tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg
     7861 tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg
     7921 attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg
     7981 cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg
     8041 atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac
     8101 attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga
     8161 acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac
     8221 aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga
     8281 gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc
     8341 caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga
     8401 gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc
     8461 catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa
     8521 tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc
     8581 ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc
     8641 gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta
     8701 tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa
     8761 aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt
     8821 ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac
     8881 tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg
     8941 cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac
     9001 ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc
     9061 ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg
     9121 tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa
     9181 caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat
     9241 ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc
     9301 ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt
     9361 ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc
     9421 aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc
     9481 taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg
     9541 taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac
     9601 cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg
     9661 ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca
     9721 aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc
     9781 cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt
     9841 cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa
     9901 gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt
     9961 gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc
    10021 actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata
    10081 ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac
    10141 tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct
    10201 ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt
    10261 tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat
    10321 ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt
    10381 ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt
    10441 ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa
    10501 ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag
    10561 aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta
    10621 gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa
    10681 ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca
    10741 aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca
    10801 tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag
    10861 ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg
    10921 tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac
    10981 ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg
    11041 cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat
    11101 cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac
    11161 caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt
    11221 ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta
    11281 gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg
    11341 tgacaatcat ttttttattc atttttttct ccccgttctt tttttattaa taaaacagca
    11401 cctaacaata aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg
    11461 aatacggacc attttcctga ttgacctgga ttgcttggac cactcggctc tttcggtttg
    11521 tccgttgttt cttttacggt ctcgttataa acacgtggtg ttgcggattc ataggcagag
    11581 actggaactt tcccggattc taattcatcc atctcctgtc cgttaactac tacatattgt
    11641 ggttggcctt ttgcatccac ccaaaattca gggatgatga cctcgattct cttacttgct
    11701 tcatccatct cgtaaccttc cacacctttt aattcttcaa aataataggt gccagaaggc
    11761 aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg aaatgaattt gcttacattt
    11821 ttgtcattca aaggatcatt gtctgctggt ttgatccatt gatttttcaa gtcattagca
    11881 ggagccatat ctaaataata tttcccccct tctatcatct gatatagagc aaaaacagca
    11941 ccttctaatg gttttccttt ttcagttgtt cctctttgac gtccatactt gaagaagtat
    12001 ggatctctta gataaccgac atttttagga tagatatgaa tctcttgcag ttcctcgctt
    12061 gattctgtcg ggtgataaat tggcaaaata gcagcaattg ggatagctgt ctgatcgata
    12121 tcaacatcca atccaacttc tggatcaatc gattcttcga aaatcaaata aactgcatcc
    12181 cgatcttggt ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc
    12241 gtatctttcc cagttgttac cgaagtccct atttggacaa gcctttcttc tgtgattagc
    12301 tgacggatat tgtttctgtt cgtttcagcg atttgctgga cgaattcttt aggagtcatg
    12361 ttttcagcag cttttttttc atgataataa tcggtcatgt cgtaaatggc aaatgtcgct
    12421 ccatttagtg gcaccgtctg tgaaatgata tctgctgttt cccctgcttc ctcagagata
    12481 gccaagccat cattttcatg gtattcaaat tgatcgttat aatcgatatc tcgaatcatc
    12541 cgtttatgca acacgaattc cacgacatct tcccctgatt cttctgccga catcgcctgt
    12601 ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa acacaacagc
    12661 tttttcatta ccttgctccc tcctttttag attgtctgac aaacaaacca taggctgctc
    12721 ctgcaagcgc catgaagaat gcagcaagca ggatatagaa taatcgtccg gatcctcctg
    12781 tagcaggcaa cggattttca ggatcgttcc cgacactaag ctgtaatctg attcgatttg
    12841 tctccaaaag ttcatattct aactcttgct gtagatcagg cgtctctgct tcttcgtttg
    12901 gataatacga aatctcaact tttagtgtac ccgcttcttt tcctggaact acccgatcat
    12961 tttctatgtc aggttcttct cgataactaa tttctaaaat aaagtaacct ggaagttttc
    13021 tatagctgtc aggtgacttc acttccttga tccgatagac cccttccaac aagtctgaag
    13081 cattgaatag ggaagaatcg tttggatctg gtgccaagcc tccctcgatg atttcatact
    13141 ctcctgcact attttgtttc tcaattgaaa attctgcgcc ttcaagacgt cggctattca
    13201 tactgtcaat tttcaaaagt tccaaatcga ttggcgctag ttcatcttct aaggttactt
    13261 gtagtacacc atcgatgatt tcatagcctt ccggcaaggg agcagtttcg tcttcgatga
    13321 ccgtatcttc aatgataaac ttaccttctt cattcagtgc aaactggaac acttgatcag
    13381 atagccgata cccatttggt gcgacagatt ccgagacctc atattcacca atagacagat
    13441 gcagaggttg gtcgtaattt aaatcgacat acttttctac tcctacggca cgagaataga
    13501 agtatccgat cccaggcaaa cgtttcgatt cttctggttc taacggatta ccagtgtatg
    13561 ctcgatacag cgggaaggaa gaatccccca ctttcctgat atcgaaacct gcaccatcta
    13621 atgtatggcc ttcatggtct aattttctaa ctcggatctc ccgataatca aacgtattgt
    13681 aagcagtgaa tgctacccat aaacgatcct cttcatctgt tgtatacgtg agatattgcc
    13741 aattttcttc acctgtttgc ttgagttgga tagtccctat agcattatcg taaagtcgca
    13801 agatccatgt gtcattttca tcaggttctt tgaaaccttc tgggttctgg ctctcaatga
    13861 ttttgtattc tgcatagttg gtagtttctt cactatcctt ataaacaaat gaatctggct
    13921 ctccgattgg atgaataact ttctcaccat ccacgattac atatttcccg tcttcatcta
    13981 ctgaatagaa cgtgagtctc cctttctctt cctctacttc gttcacacca gaaagccctt
    14041 tcgctactgg atatccgatt tccagtccgt taccatctgt ttcataaagt tcaaactctg
    14101 cgccattgat tggcatatta ctgccttcaa ttagtttatt gatattcatg tggattggct
    14161 tgaattcatt tcgcatttcc agtttgacat acaactcatc atactctgtg ccttctatgt
    14221 taggctcttg tacaacagaa agcttgtgat attctccttc ataataggaa ttcggatctt
    14281 taccttccgg tccgccgact ctcgaaccag atgtatattc atctacaagc tcgtagtgac
    14341 ctaacatctc atcttcgttg gctgcatatt ttttgatttg gaattgatac gtctcatata
    14401 ctaactgata acgtggtggt ccagcgatct ctctgatcca aaatatgtcg ctgactgctc
    14461 cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat cgctattccg tccgtggtag
    14521 ttttacttgt ttcgattggt tcggcttccc cactctctgg attttggtca tataatgcaa
    14581 attcgactcc agccaacggc tcgccatttt gacccttctt gctgacttgg aaattacggc
    14641 ctttatgttc gtaacgaatg gaagaagatg ggaaatacat ccgtccagta gtgatatcat
    14701 tctcataatc ctccaggttc tgaatatagg ccatattgtt cactgtgtaa aaatttccgt
    14761 cttgagctcc cgcatcaagt tttacttggt acttgatcct gaccatttcc ccttcaccta
    14821 aagaaatcgg agaaaattta taagcttggt tttcccgtcc gacatacgtt gtcggagtcg
    14881 ccactacttc tggtggggct tgtccatgac gttcttcatc ccatatttct ggctcttggt
    14941 acttcatgcc gttttctcct ggatctagat ggtaaaactg aatatctgcc gatctgccag
    15001 ctccaaatgg tgagacatta aaaccagttt catcataaag tgtggcttgc tggatcgttc
    15061 tcttgaaggt agaagagata tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat
    15121 tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc aatatttttc aatacatttc
    15181 gtccaatagc atctagtcgt tgtctcccgc taacgtttcc atcgattcct aaaccaatgg
    15241 agaagatact tggcgggttc tcttgatact tgtttttcag ccaatgatga taagcaactg
    15301 tgttgatcgc agatgacttt tcttgcgaag taggactgcc attcgtattg ttattagaag
    15361 aaccatcacc aacccagtga gtcgctttat tatctccttc ttttacctct ccataacgat
    15421 agtgtacatt tacttcatct tctggacggt taaagtcaga tgcataggta actggatgag
    15481 taaaccctgc attagaagta ttcccagaaa actcctcaaa atttctgaac aaaacgtcat
    15541 tatcttcagc aatcttatct tccatcacag tccagttgct ccaagctcca ttagtgctcg
    15601 tacgataact tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct cccacaacga
    15661 ttaggatttt ttctggattt ctattttctc cgccattgtc tttatacaat gtttcatagc
    15721 catttttaag ccctagtgta agcggagttc ctccgattgg tgtacgattt aatgctgtat
    15781 tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt
    15841 gctcatggtt attgatagct gtactattat ggtaaaaatt caccataccg attcgaatat
    15901 ccagactcgg gtcagagagc agatctttac tcatttcgtt tactgcctca atcaacgcag
    15961 catttttagt ttgactattg gtcccagcgg ttaattcact catactcgct gacttatcta
    16021 gaacaaaaac gatatcgatt ggactgattt cctttttctc gcccccgatg acatctagat
    16081 agacattgta aagggttgtt gcatcttcag gatgtagatc agatgctgtg ctgcttggca
    16141 gaacaggttc tacccatttt ttgatgtacg ccccgttata atccaagtaa ctgttttcaa
    16201 aattccgttc atttccccaa agctgaacgg tttgagctgg aacgtttcta tcttcttcgc
    16261 ctgcagttga tccattatta gtagcatagt cgtaatttcg ccagttcgct cccccgtttg
    16321 tataatcact atcatcgatc aagttctctc cgtcatatgg cgagcctttg tctccttgat
    16381 agcgttcatt gctttgtgtc caatttccta acacttttgt atagtcattc gttgtaaatt
    16441 tcgggtagcg atgctgttcg ttttcaactt ctccgctagg atagatatat tcataggggt
    16501 cttgcgtagc tgttggccca agacgctcca aaataccgtt gaataactgg cgttgaatgc
    16561 gctggagtgc ttgaccgctg ggtacgattg gttcgtccag cttttccatc tcattttctt
    16621 cgctacgttg aatttcagcg gaatcttctg tatcagtttc attttcttgt aattcttggt
    16681 tatctattct ttcatcaacc accacttgaa gaggtagttc cccttcgttc acatcgggga
    16741 tcttttctgt ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa acttttccga
    16801 cataccattc ttgaccatcg atcgtctcga cttttaggtc ttctccatct tcatcatagg
    16861 aggatagacc tgttccacga acgtttttta cggtaccaat cccggcggct gccgcatcga
    16921 ttgctaaacg tattctacct tcattggcac tttccttttt ttcaaaagcc agatgccatt
    16981 caagggtttc ttctttttca atatacttcc cttcaatttt tgcatagtcc tcatctaaga
    17041 atacctcatc ctcctttgtt tctgttgact cagcagctac atccagggtc gtcatatgat
    17101 agactgactg gaaatatggt ccaaatagca aactgacagt tactaactgc aaaagcttca
    17161 attttaaccc tttcacatcc ttatactcct ttcaaaaata tttgtcttct ttcacaacaa
    17221 tcaacaaaac aatgcccatc taattataat ttaatttaat tttaatttta aatcaaatga
    17281 tatccttttt
//
LOCUS       DAHHPA010000060        17184 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.061, whole genome shotgun sequence.
ACCESSION   DAHHPA010000060 DAHHPA010000000
VERSION     DAHHPA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.061"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            961..1641
                     /locus_tag="NBH55_001842"
     CDS             961..1641
                     /locus_tag="NBH55_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCD1509824.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            1670..2983
                     /locus_tag="NBH55_001843"
     CDS             1670..2983
                     /locus_tag="NBH55_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCD1509825.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            3789..4556
                     /locus_tag="NBH55_001844"
     CDS             3789..4556
                     /locus_tag="NBH55_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCD1509826.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            4583..5584
                     /locus_tag="NBH55_001845"
     CDS             4583..5584
                     /locus_tag="NBH55_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCD1509827.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            5607..6893
                     /locus_tag="NBH55_001846"
     CDS             5607..6893
                     /locus_tag="NBH55_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HCD1509828.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            6927..7580
                     /locus_tag="NBH55_001847"
     CDS             6927..7580
                     /locus_tag="NBH55_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCD1509829.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            7613..8818
                     /locus_tag="NBH55_001848"
     CDS             7613..8818
                     /locus_tag="NBH55_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1509830.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VWCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            8948..10273
                     /locus_tag="NBH55_001849"
     CDS             8948..10273
                     /locus_tag="NBH55_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509831.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            10290..11420
                     /locus_tag="NBH55_001850"
     CDS             10290..11420
                     /locus_tag="NBH55_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509832.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADSEK
                     LLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            11446..12531
                     /locus_tag="NBH55_001851"
     CDS             11446..12531
                     /locus_tag="NBH55_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HCD1509833.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            12554..13369
                     /locus_tag="NBH55_001852"
     CDS             12554..13369
                     /locus_tag="NBH55_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509834.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            13353..15071
                     /locus_tag="NBH55_001853"
     CDS             13353..15071
                     /locus_tag="NBH55_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HCD1509835.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            15097..15933
                     /locus_tag="NBH55_001854"
     CDS             15097..15933
                     /locus_tag="NBH55_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1509836.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            15938..>17184
                     /locus_tag="NBH55_001855"
     CDS             15938..>17184
                     /locus_tag="NBH55_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCD1509837.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENG"
BASE COUNT         5592 a         2384 c         3765 g         5443 t
ORIGIN      
        1 acaaaagtat attgatagcc attaataaat tttccaaaaa ataatgacag cacaccaatt
       61 tttataattt agcttgttga atcggtatgt tcatgggacg gttcaaaaaa agaatcgaaa
      121 aaataaacgg taaaatttta gtgaatccta tgtaataaaa tttggataga tggaaaaaca
      181 gcacataagg aggctgagtt tgttgtcatt tctgctccta caaactatgg tagtgaatgt
      241 gttattgaat tagtgatgtc tgttacttca gagacattgg ggataattaa cttcactata
      301 cctgtagggt atacaaaaaa tggaagaaaa aatattatca atagatttta tttagttcag
      361 aatttttacg tggaagtaaa ttattgatga taatctttat ccaattagaa ttattatcgt
      421 aggatgtgat gatgatagcc gtgaagttgt tcaagtattt accaatgtct acaacgcaga
      481 aaattagttt gggtttttcg gattggaaaa tttcaatgta taaaatcata aatttgctgt
      541 aaatagacag tttggggtag taatagccca ataaattgtt cgataaactc atctttgggg
      601 tgagaatagc tcaatcattg aatttcaaac ctgcctttgg ggtgggatta gaggtcaata
      661 tgtacctaca taatactttt tggaaaatga tggtgttgag aatgtaagta catttcaatg
      721 tgaaattcta atatagcccc aaacgaggtc aataaaaatg acctaaactc gtatttctcc
      781 cctaaacgag gaggagatag atatgaaaat tgaatgtgcc ggcgttggag ttggctgctt
      841 ttagtaggaa tgaacatggt tcgatgagaa ctgtttcaga tagagtggtc gatgatcaca
      901 ggcctttaag tcgtttacag atgatgagac ttgtaaaaat agagggacga atggaaaaag
      961 atgattggta tagaagaaaa gaaaggtgtt cagaaagacg taacaattga tagggaagag
     1021 aatcttaggg gacatagatt ctattggatt ggacgcagag cacaagatat cttcttctcg
     1081 gctctagctt tgattgcatt gagccctgtg atgctaatta cagcgctcgc cattgtgatt
     1141 gacgatcccg gtggcagccc gatttttgtg caggatagag ttggaagaaa tggaaagctg
     1201 ttcaagtttt ataaattccg atctatgtgt gtagatgctg aagcaaaatt agaagccttg
     1261 ttggcacaaa acgagatgga tggtccggtc ttcaagatta aagatgaccc aagaatcacg
     1321 agagttggta aatttattcg aaagacaagt atcgatgagt tacctcagct ctggaacatt
     1381 cttaaaggtg atatgagtat tattggaccc agacctgctt taccgagaga ggtggaacaa
     1441 tacggtgact acgaaaggca gcggttgttc gtacagcctg gtcttagttg ctattggcag
     1501 attgctccac atcgtaatga actgtccttt gaagaatggg tggaacttga tgtgaagtac
     1561 atcaatgaaa gaagctttat cacggactgg aagattatct tcttgacttt acaggctgta
     1621 ttatttggat atggtgaatg attatatata gtattaggag ttgaaatatg tgaatattat
     1681 tttattatcg ggtggtagcg gtaggcggtt atggccgctg tccaacgata ttcgtagcaa
     1741 acaattcatc aaactgttta aggatgaaaa tggcgaatat gaatctatga ttcaaagagt
     1801 ttatcgccag attacaactg ttgacccaga tgcaaaggtc actattgcaa caagtaagtc
     1861 tcaggctagt gcaatccata atcagttggg agataaggct tcgatttgtg tagaaccttg
     1921 tcgtagagat actttccctg caattttgtt agctgcagca tatcttcacg atgaacttgg
     1981 cgtaggtgat gatgaaagtg ttgcggtttg tcctgttgat ccttatgtag ataattccta
     2041 ttacgaaggc gttgaagccc ttgagaagtt ggtgcagcag aatacggcta atttgacttt
     2101 gatgggtatt gcacctacat atcctagtga aaagtacggt tatatcatcc ctgaaagttc
     2161 taacgaagta agtactgtta aggagtttaa ggaaaaacct gacaaagcaa cggcagagaa
     2221 atatttagct cagaatgctg tttggaatgc cggtgtattt gcgttcaaac ttggttacct
     2281 gttggaaaaa gctcacgcaa tggtggattt tgaagattat cgtgatttgt attccaagta
     2341 tgagacttta accaagatca gctttgacta tgctgtggtc gaaaaagaaa cgagcattca
     2401 agtgatgcgt tataacgggg attggaaaga tgtcggaact tggaacatga tggctgaagt
     2461 tatggctgat aatattaagg ggaaagctgt aatggatgat acctgtgtta atactcagat
     2521 tgtgaatgag ttgaatattc ctattcttgc tatgggctgc aaagatatgg taattgcagc
     2581 aagtggcgat ggaattctga tttctgataa agagcgcagt ggctatatga agccgtatgt
     2641 tgagaaaatc agtacggaag taatgtttgc agaaaaatcc tggggtacat ataccgttat
     2701 ggacgttcag gaaggctcca tgactgttaa agtttctatg caggccggac agaagttgag
     2761 ctaccactac cacaattctc gcgatgaagt ttggactgtt gtagcaggac agggtaaagc
     2821 gattgtagat ggtatgaagc agattcttcg tgcgggtgat gtggtgacta ttgccgcagg
     2881 atgtaaacat acaatcgaag ccgtgacaaa tctggatatt atcgaagttc agattggtga
     2941 aactattagc gtcgctgaca agtataagag ttctttgaac taaaagttcg cgaggtagtt
     3001 attatggaga tactaaaatt tcaacccgtg ggtaaagatt atctttgggg tggagaacgg
     3061 tttaagacgg aatttggaaa aaattgactt aacaccatta agcttatacg tgggaatgct
     3121 tagttcatct agacgataac tacgtacgga aatatcaaat atgaaggaca gaatggtaaa
     3181 accgagttgt ggtatgtatt ggaagctaca ccggggggtg atttatgctt ttgagtacga
     3241 tgtaacagaa gaaatgttac gaaatgctgt gatggacaga tcacttggga aacatttata
     3301 taaagaagaa gtacacaagg gagatctttt ttatgtcagt tcatggtatt ggcgctggag
     3361 tattagtggc tgaaatacag gaaagctcaa atgttaccta tcgagtttac gattatgatc
     3421 gagtagataa gaatgataat aagtgagaat tgtactttga aaaagcgatt caggtaatga
     3481 atatggaagc cactttgaaa ttggagaaaa gaaataagct cattcatttt tatcctggtg
     3541 gctctcgtga aatactgtgt agatgtaaat actgtgagcc cgaaagaatc caagtaaaga
     3601 cggggttttc gttctcggta aaagaggaat cttttcaagt tcttttatgt atatcggggg
     3661 acggacaagt tgagtcggca aatggtgtta tgaaactcat tagatttgta aaaaagatat
     3721 tgtatgtttt tgccaacgaa tattggaaga tgttaagtgt tgagcgatgc ggacttactg
     3781 aaaattagat ggggatagag ggaggatatc taatgtcacg aatgagattt atgaacacag
     3841 aaattgacaa tcttacaatg ggtgaagtat tggtagaggt tgaaaaaatg attcaagata
     3901 ggagatgcgg atatgttgta actccgaatg tggatcacat tgttcagttg gaaacaggaa
     3961 aagagttgca agatgtttat aaaaacgcat ctttaatttt gacggatggt aaaccattga
     4021 tttggatttc aaagttttat aagacaccaa taaaagaaaa aatatcggga tccgatatat
     4081 tttctttact gtgcaagatg gctgcagaaa agggatacac aatgtatttt ttaggtgcgg
     4141 cagagggcgt tgcggctaaa gcggcagata acctgatgaa aagatttccg ggattaaatg
     4201 ttgtaggaac atactctcct ccttatgggt tcgaaaaaga cgaagatgaa atgtttaaaa
     4261 ttgattccca aattaaacga gcaaatccag atatgcttat tgtgggtttg ggttgcccta
     4321 aacaggaaaa atttatgttt tataactgtg atagattggg agtacctgtg tcttttggtc
     4381 taggagctag tttggacttt gaggcaggga atgttaaaag agcaccaaaa tggatgtcag
     4441 attatggatt tgagtggctg tttagaatta ctcaggaccc taaaagaatg gcaaaacgat
     4501 atttggttga tgatttaaaa atctttaaat tagcactaaa atatcggaaa aaataaacta
     4561 tattcaatga ggacaatgaa gtatgattat tacaaaaaca ccttttcgca tgtctttttt
     4621 tggcggcgga acggatatgg aagattattt tcgagaaaat ggcggtgcag tgctttccac
     4681 tacttttgat aagtactgtt atgtgaatgt gcgtcatttg cctagatttt ttgattacag
     4741 cacagagtta tcatattctc gtactgagag agtaacaaat attgaagata tcgagcatcc
     4801 tgcaattcgt aatgcgatga aaatgctaga tatgcatgaa attcgcctta catatgaagc
     4861 agatcttcct gcacgttctg gtctgggtac atctagttct tttgccgtag gtatgttgaa
     4921 tgctttctat gctttgaagg gcaagtacgc tgacaagaag aaactcgttg ataaagcaat
     4981 ttatcttgaa agagtccttt gtaatgaagc aggcggatgg caagatcaga ttgcagcagc
     5041 ttatggtggc tttaaccgaa tcaatttcaa ctcagatggc tatgaagtat tgcctattat
     5101 tattacgcct gagcgtaaaa agcagcttaa caacaacttg atgatgttct ttactggatt
     5161 caccagattt tcctctgatg ttcagaaggc aaataatgtc agcggcacag aggacaaaag
     5221 agtaagactg aaaaaaatgt atgagcttgt ggatgaagca gaagcggttc tgactgataa
     5281 gaacagagat ctagatgatt tcggcagact gcttgatctc acttggagat tgaaaaaggc
     5341 taccggtggt gcgattagca ctggaagtat cgatgaactt tatgaaaagg gcatggctgc
     5401 tggtgcattg ggcggtaaac ttctcggagc aggtggcggc ggtttcttgg ttttttatgt
     5461 tcagcctgaa aaacaaaatg ctgtgcgttc ggcaatgagt aatttgatgt atattccgtt
     5521 tgaatttgaa aacggtggaa cacaggttat tcattacaca ccggaaatgt atattccaaa
     5581 ataaagaaat cagcgaggat aagaatatga aagttgttat tatggcaggc ggtaagggaa
     5641 ctcgcatctc atcggttgca agcgatattc caaagcccat gattaaaatt gaaaacaaac
     5701 cggtattgga gcacgaaatc gaatgtctga gagaccaggg attcacagat attattctta
     5761 ccgttagcca tcttggcgat atcattatga actattttgg cgatggttct ggaatttcac
     5821 cagttactgg tcagcccttt gacgttaaaa ttgaatactt tttggagaaa gagccattag
     5881 gaaatgccgg tgcattgttt cgtttaaagg ataatttgac cgaagacttc ctcctgctga
     5941 atgcagatgc gatgtttgac gttgatttca accgatttgt agcatatcac cgtgaaaagg
     6001 gtggcttggt gacattgttt actcacccta acagtcatcc atatgacagc ggtctaatca
     6061 ttgcagataa ggatggctct gtagacagat ggcttgtaaa ggaagattcc cgtccaactt
     6121 attaccgtaa cagagtaaat gcgggactcc atgtgattag tcctgctgtt cttgatgtcg
     6181 agataaacac acctaagatt gacttggacc gtcagttatt aaagccgttg gcaggaaccg
     6241 gaaaaatgtt ctgctatgat agtccagaat acgtaaaaga catgggtacc cctgatcgtt
     6301 attatgctgt ctgtgaagat tttaaggctg gaagagtgaa gggcaaaaat cttcagaata
     6361 agcagaaagc tgttttcctt gatcgtgatg gaacgattaa tcaatatgtt ggctttctgc
     6421 gtcatattga tgagttcgaa ctaatcaagg gtactgaaga tgcagtgaaa aagatcaatg
     6481 ccgctggata tctggctatt gtaataacga atcagccagt aattgcaaga ggtgaagttt
     6541 cttttgaaga attggaagag atccataaca aaatggagac acttcttgga tataagggcg
     6601 cttatttgga tgcagtttat tattgcccgc atcatccaca taagggatat gaggaagagc
     6661 gcccagagct aaaattcgat tgtgaatgca gaaagccaaa gcccggaatg ttaattcagg
     6721 cagctacgga cttcaacata gatttgagtc agtcttggat gattggcgat ggcgagaatg
     6781 atattaaagc gggccaagcc gccggctgta agactgcatt ggttggcgag agtgagtatg
     6841 gacaggatat gaccatagca accctgagtg aatttgcaga taagatctgc taagggatgt
     6901 tttatcacga aaggagagta taaacaatga tagacaatag attacagaaa cacattgatt
     6961 tgctgattaa acgctatccc actcttgaag taatcaagca ggatattgtt gacggatacc
     7021 tgattatgga ggaatgctat ttaaatgatg gcaagctgct gatagcagga aatggcggat
     7081 ctgctgctga ttccgagcat attgcgggtg agctgatgaa aagctttaag acgcctcgcc
     7141 ctgttagtag ggaatttgca gataaattga ttgcggttga tcctgtaaag ggttctgaat
     7201 tagctaagaa tcttgaatat ggtttgatgg cgattcctct cgttgctcat gaagcgcttt
     7261 ccactgcgta tattaatgat gttgatggcc taggtgtttt cgcacagcag ttgtttggat
     7321 ttggaagacc tggcgatgta ttccttggta tttcgacatc cggcaatagc aaaaatgtga
     7381 taaatgcaac tgttgttgct agagctcttg gtatgaaagt aattggctta actggagcca
     7441 aaggtggaga acttgcaagg gtagcagatg tttgtgttaa agctcctgaa acagaaacat
     7501 atatgattca ggagtaccat ttgccaattt accactgttg gtgtctaatg ttggaagata
     7561 aattttttgg gaatagataa aatatctaag cccatattga aaggataaaa ttatgaatgt
     7621 actatttata acaactcaat ttccatatcc tctagataat ggcggaaaaa ttggtgcgta
     7681 caacggtatc agtgttttat ctagagacta cgatgtgacg gtattgtctt tcaccgaaga
     7741 accgcagtat gttgaagaag gtgtcagttt ttttcgagag aaatttaata gtgtaaaatt
     7801 tgaaaatcct gtgatacatg atgtacacat aagaaataat ccaatgaaat tggtagaagc
     7861 tttagcaata gggtattgtc tacgccaacc ttatatagtt tcaaagtttt atgattcaaa
     7921 aatgctgcag ttaatagatg aaaaattttc taacaatgca tattgggata tcatttttat
     7981 tgactatctg aatatgcaaa gttatggtca atacatacgg gagaagtata aaacacaatt
     8041 tggtaagtat atcttaaaag accacaacat agaatatgat ttagtcctac aggaagctga
     8101 atcaagtcga ggtttaaaaa agaagttgct tcagttagag tataagcgta ctcgaaagta
     8161 tgaagaggcc gcagtaaata gggccgatta ttgctttagt gtgtgtgatg caaatacgga
     8221 agtactgaaa aagattaatg acaacagtta tacgatgctt ccaacttatg aaatgcttac
     8281 aggggtgaaa aaaaatatag agaagaacac gatcctatat ttgggtaacc tttcttggaa
     8341 atccaatatg caaggattag tttggttttt agagtcaatt tatccaaata ttcaacaaag
     8401 aattccggat gtaaaaatca caattgtcgg tagcgggcca gttggaacag attttagtaa
     8461 atattcaggc gtggattatc gtgggtatgt aaaagatatt tcacacattt atgacgatca
     8521 agcggtgcta attgtacctt tgttagaagg tagtggtata agaataaaaa tattagaagc
     8581 gttcaataat gaaattgcag ttgtatctac attagtttgg tgtgatacga ttggcgcaaa
     8641 agatggaagt gagttattta tagcagataa cgaaaaagat tttgcaacgg ctgtagttag
     8701 actgcttgaa gacatgaatt tgaatgaaaa agtaagaaag aatgcaaaat tattcttaaa
     8761 agaaaaatac tccttaacgg ctagacaaga tgagtttagt gcgataatta acagctaata
     8821 tatatataag cttatctgat gatgatttat aggaggaaat atatggagat ggctgagaga
     8881 aaggaaaaga aacttggaaa taagaatatc atattatttt agaaaactcc tgttatttta
     8941 tgtttgtgtg attgttgcgg ctatgagtta ccaccttaca ggcaattcca atgtagcgtc
     9001 attaattgct gtagtaggag tagtttatgc tattgtaaag gcgtgtaata acgagtatct
     9061 ggctatatgg gttatgtttg tattaggttc tgatatatgg gggatagtgc catatattaa
     9121 cattggtagt ggtgtgttgc attggtcaga ttttaatgtt atagtagcga tatttctctg
     9181 cttgagattc atcaaagtta aagctaggag tagatacggg attgtctata gtgtaatgct
     9241 tatatttatt ttgattgcat gtgtgcaatc gcattcaatc tatggacaag atgtacatac
     9301 aacgctctta acagctagag aattgctagt tgtaatatcc ttttggccga tagtaaaaat
     9361 agtatattca gaaagaataa ctaaaaatga attcattgat ttagtgtgta aattcacaag
     9421 atggacaatg tgtatatatt ggtttgaatg gattcttgtt aatatgggat tcaatataac
     9481 ctttttacag acaggtgcac gctggggtac tagaatctat atagatccga tatttatcat
     9541 tttatatttc tttataaatc tatatgcggt tatatataaa agagagggct ataatagtac
     9601 aaataaaagt atatggaatt gcatttttag tttactgaca tttgttataa tctcgcaagg
     9661 cagaagtggt atattatatg tagtaataac ctcggttatt atgcttttaa tttcgcttaa
     9721 accatccaag attattagat ttggtatatt aggaattgcc ttaattgcat gtctagtgtc
     9781 tgtgacccaa attagagagg tcgttatatc ttcgtttgta gagtctcaga gcagtgacac
     9841 aggtaatatc gcatatagaa ttgctgaaca agattatttt gaaaataaat tagaaggtca
     9901 tgaactattt ggagttggta taccaaacaa tcatgatgcc agagctgtag aatattcagg
     9961 caaaacaata gattttgagt ctaaaaaata taatccctat tatcttgagg acttaggcgc
    10021 ttttgaaatt cgttatagat ttggtataat tgcatatgcg ttatatatag ttgctttagc
    10081 tataagtatt atagatgccg gatttcgaag agacaaatcg gtcgccgcat ttgcgggtat
    10141 ggcattcatg gtgtttatgg cgcttaattt taatttgcta gcgtatatta cagcaagacc
    10201 gttcggattt atgatttctt taattttgat agaaatatcg gcattcaatg atgaattcat
    10261 atatattgaa taggtaaggg agagcacaga tgaatttaga tcgattaaag aaaatagcaa
    10321 atatgatttc accaatcata aaagtgataa atgcgccgtt agcgtatttt agtgggaaat
    10381 cagattatat ggagaaggga atattgtggc ctactgtaac aacctactcc aataacagta
    10441 gaaaagtgat tcaatataca actttaccta aaaacgtaga caacatggct caaatgcaat
    10501 gggctgggga aagttttgag gacatatgta tagttcttca aggtccagta ttagaagacg
    10561 gtgattttac actaaatacc gttaaaatgt ataataaata ctatcctaaa gtaaaacttg
    10621 tagtgtctac ttggatagga ttatctgaag agacagaatg caagttgcgt gaagctggtg
    10681 cagatgttgt attatgcaag aaacctgaaa aaacaggtgc gtacaatatt aatctacaac
    10741 ttacttctgc atacgaaggc gtaaaaatgg cgcaaaaact ggggtgtagt tatgcgctga
    10801 aaacgcgaac tgatcaaaga ctatatgcaa atgatgtact aaagtatttc caaacattat
    10861 tgaatatttt tccatcttcg gactgtaaag tagtgaatca gcgattaata tttacgactt
    10921 atggaaaatc atttagatat cttccgttta atttatgtga ttttttggta tacggagaaa
    10981 ctagtgaatt attaaaatta tatggtattc aaagagataa ccgagcatca gactttggga
    11041 aaattattta tactgaggaa aaagagttta ataaaaagat atttacacta ctagaaggaa
    11101 aacaaggtgt atctccatat gatgtttttc cagattttga tgcactatat aaaaaatata
    11161 tgtttgcaga atactatatt gtgtataatt atttcctgga aaatattgca agtaatgaat
    11221 cttatggtga cttaatggat gcctattata gttatttaaa gaaatatgca gttgttgctg
    11281 attcagaaaa attgttacta tattggccga aatatgttca atataatgtt aagttcgaaa
    11341 atgaacttga tttctatgcg aagttggatt caaaaaaatg gatggatatt tatgctaatt
    11401 atacaccttt tagtcagtga ttatatggaa aggatgaggt gaataatgaa aatcggaata
    11461 gtgactattg ttaatggggc aagttttggt aacagattac aaaattatgc gactcaaaaa
    11521 gtgttggaaa ataacggcta tacagttgag acaattaata attgttttga caaagaaagt
    11581 accctatcca ttgttatcaa aaacgctatt tatacattat ttaagtttat accgcatttc
    11641 aaaaagtcaa aaagtatctg gaaaagaatg tatacatttt atcaatggga caagcagtat
    11701 atagattatt ctcatcgtaa aattgatgca tctaaaaaaa acagctttgt gagtataaag
    11761 aatgattatg actatttctt ggttggtagc gatcaaattt ggaatccaaa atatgcagcc
    11821 aataagggat ttgaactttt atcatttgtt ccaaatgaaa aaagagtttc attatcaacc
    11881 agtttgggag ttccatctat accaagtgat ttagaaaaat tatatgtgac agagtggaat
    11941 aagtttaaat caatatcggt acgtgaaaat caagcggcaa aaataattga ggacttgaca
    12001 ggcacaactg cggctgttca cattgaccca actttaatgt tggagcgttc ggaatgggaa
    12061 gcctttcaaa aaaaaccaga caatattgat gaaaattaca tcttgtcgtt tttcttaact
    12121 aatccgccta aggatactgt tgatgtatta gataaggtcg ctaaagattt gaaaataggt
    12181 gttctcacct tagatgcgtc ttgggaatat tttcaagata ttactccgaa tcattttgtt
    12241 tatttgattt cacatgcaag aatgatttat acagattcat tccactgcac agttttttca
    12301 ttgctatttg gaaaaccggt tgtggtattt gaccgttttg atagtgaaaa aatgaattct
    12361 aggttaagta cattgttaaa taaagtaaat aaagcacatt tttgggtagg aaaaaaagct
    12421 gatgaagcaa tgattcgtgc aatagcatct gaacctgaat atgacataga taagctgatg
    12481 gtatcagaga gggaatgtgt aaaagcttat cttaaagagt atttacagta aaattttgaa
    12541 atgggatgaa aatatggagc atttaagtga ttgttttggt aaaatgaaga ttgttacgac
    12601 ttcatgcgca agagaatatt ttaaggatga tttggataaa tcagagtatt catgttttgt
    12661 tgattctcga gcagctatgt ttttcgcaat gggacaagct ttagctaaaa gaaggaatgt
    12721 agttgtaatg cttgctgatg aattgacatc gtgttatacg gcactaacag aatgtgcatt
    12781 tcaacaagtt gatttaattg tgtttgcttt tcagacagtc agacatgaaa ttgatttagc
    12841 atctttgaag aattgtggtt gcaatctaat taactgtagc gcccaagacg atattgatag
    12901 agttgtgtcc aacactaagg ggttaaatca gacaacagta attaaaatca atatgaattg
    12961 tgagtcggat aaagatcata tatgtgagta tatagacctg ccagacatac ctgttttttt
    13021 ccacggtagc ttatactcac ctgcaagtaa aaagaaatat gagcagtatg gacaggtatc
    13081 gaagtattta ggctatctcg tggggggaaa agatgcaata ctcataattt ctcacaatga
    13141 gtttttaaac gatatcagtg tatggattaa ttattattct tttggagaaa ttcaacccaa
    13201 aattattata tttgacagtc cgaatgacta tcaatataaa tggctgtgtg acttagaatg
    13261 cagtgtagct cattcaacca accgattttt gtctgaggat ttagccttat tgcaaaataa
    13321 cggaatttcc attgtgttaa gaggtgataa ttatgatatc gtcagttaaa aatgttcaaa
    13381 ttttagtatc tttattaaaa cagcataata ttaaagatat agttatctcg ccaggtagta
    13441 gagattttca attggtgcat agtgtagaaa cagacagttt ttttagaacc tatacggttg
    13501 tagatgagcg cagtgctgcc ttttttgcta atggactttc tatggcgatt gataagcccg
    13561 tgtgtgtatg ttgtacttct tcaactgcct cttcaaatta tatggcggca gctaaagatg
    13621 cttttgctaa tggcatccaa cttgtacttc ttactgctga tagagattac cgccgcttat
    13681 atcagatgga agatcagatg attgatcagg tagatatgta tggggcttat gtgcgttcta
    13741 acgttgatat tcctattgtt aaggattctg atgatgaatg gtatgctacc agaaaaataa
    13801 atgaagcaat attggagtta aatcatggcg ttaagggtcc cgtgcagatt aattatcaat
    13861 taatggattt aagtgcgccg tttgttgaga ccatgccaga gtatcgtaaa attacaagat
    13921 atcaagagga tgatttactg tcatcagtag agatttttaa acagaaacta ttatcaaaaa
    13981 agagaataat ggttttatgt ggacagcagg aagaaaataa tttaagtgcc ttattggaag
    14041 agttctttat taaatataat acaattataa tcaaggataa ctattctaat atacacggtg
    14101 aacactttat taagactgtt ctagtaacag aaagaatgag tgtaaatgca tttattgatt
    14161 attgcccaga tttggtaata actattggta aacatgtatg gtcttttgtg aaatatactc
    14221 ttagagataa aaagcttaaa tttgagcatt ggcggatatc tgaggatggt aagattgttg
    14281 atccgttcaa caagctaaca tctgtctttc agtgctcagc ggaatttttt atggagactg
    14341 tcactgctgg tgtgaatagc aataataatc ttgagtatta taatctatgg aagacaagat
    14401 tatcaaaagt ttgctatcca gagttagtct atagcaattt ttatgcaatt tcagaactag
    14461 taaaaaaaat tccagatagt gcattagttc attctagtat tttaaatgcg gctagattgt
    14521 atgactttgt atctgatgat aaagcattac tcagttttgc taatttaggg gctgatggta
    14581 ttgatggttg tttaccaaca tttttaggtc aggcagaagc agataaaact cgacctgcgt
    14641 atttaatttc aggagacttg agtttctttt atgctatgaa tagtttatgt ggtgacattc
    14701 cagataatgc acatattttc cttattaaca atcattctgg aagtgaattc catacaaatt
    14761 ttggtaagtt ttacaacgga tcattaagtg ttgataagca tatagctgca ggacatattt
    14821 caaacgcaaa ggattgggca ttagaaaata atataaaata cattactgcg gattctaagg
    14881 aaagtctcaa atcggggttg gatgtattta ttaatacatt tgacagtcct gttttatttg
    14941 aggtctttac agatccggat acggatacgt ctgttctgga atctttttat agtataaata
    15001 aggtaatcac accaaaaatg tattttagaa aggcgttaga tattataaaa aagcatttac
    15061 acattaagta gatgtttatt gcgaggtgaa acaaatatgg caataaaatc ttatattaaa
    15121 cggttattgc tttatatccg tcatggagat cccaaaataa atttagatgt gagttattta
    15181 tctgaaggtg caatgcttgc tggtaagact gtgataatta cgggtggtgc aaaaggactt
    15241 ggttttgcaa ttgcaaaatc atttatagct caaggcgcaa gcgttattat tacaggaaga
    15301 gacatggatt cgttgaataa tgctaaatgt cagcttggag atatgtgttc tatcagggcg
    15361 tttgatataa ctgatgctaa aatgtatcga acgttttttg aagatgcata cgagaccttt
    15421 ggacgtattg atatactgct taataatgct gggatctcgt tacacgaaga tggatttatg
    15481 agtgttactg agaaaacttg ggataagcaa atggatacaa atttgaaagg tgtttatttc
    15541 tttacacagg aatttattaa gtttgttaaa gaaaagaatc aaatgcaagc aaatattatt
    15601 atgatgtcct cagaaagagg cttgtgtggt gatagtattc cgtatggatt atcaaaggct
    15661 gcaattaact ctttagttgt aggcttgtct aagaaatata tttctgaggg aattagggtt
    15721 aatggtattg ctcctggagt aactgcaacc gctatgactg gtatttctga aaagggtaat
    15781 ttgtataatc ctgcaacgat cggttataga tattttctcc cagaagaagt tgctcaagtt
    15841 gctttgtttt tggcaagtag tctttcaaac tgtatcagtg gtgaaatcat agcctgtgac
    15901 caggggcaat atttaagtcg aaattggggg taataacttg ggaatattat taaaaaaata
    15961 taatagcctt agtgagcctg caaaagcatc tatttggttt acactttgta atgtgttgca
    16021 aaaaggaatt ctatttttga ctacacctat ttttacaaga atgctttcaa ctgcagaata
    16081 tggtaagtat acgattttta tatcgtggta ttctattata gcaattttca caacgttgaa
    16141 tttatctgca ggtgtctata ataaaggact aattaaatac gagaataaat atgcattaac
    16201 ctcgtcattt ttaggattat cgtcatttat ttccctttgt gcattgatgc tataccttcc
    16261 atttagaacg gttataaatg ggctactgga tttatcgacg gagtttgtca tattgattct
    16321 tattgaatca atatttgagc cggctttttt gctttggtca gttaaggaac gatttgatta
    16381 caagtatcaa aaactagtac tcatcacagt tcttatggct gttttaaatc caggtctcgg
    16441 tattatatta atcttatcat ttggggagta tgctatttgg agagtacgag cttatgtcgt
    16501 ggtaaatgtt gtagtgggga tgtttattgg cataggaata ctggctaagg gtaagaaatt
    16561 atttgataaa gagtactgga catatgcgct cagatttaat ttgccattaa taccacatta
    16621 tttatcaatg acattgctga atcaggttga tcgccttatg ataaatagta tggttggagc
    16681 tagcgaggct gcgatttata gtatcgctta cactatatct agtatgatga atatcataac
    16741 aacagctata aacggttctt ttattccttt tacatacaaa gcaattaaag atggcgtgta
    16801 tgaaaaaatt aaatccgttg ctaatcaact attagtgttg atttttgtct tgtgttcgtt
    16861 tactatgctg ttcgggccag aattgatttg ggttgtcgga ggagaaaaat atatggatgc
    16921 tgtttggatt attcctccag ttgcggcatc agtatttttc atgtttttat atccgctttt
    16981 tggcaatatt gaattttact atgaaaagac aaactacgta atgtttgcat cttgtgtggg
    17041 ctctatcgca aaaattattt caaactatgt tagtattccc cgttttggat atatttcggc
    17101 tggttacacg acattgattt gctatatgtt gttttccttt gcccactaca tgttttataa
    17161 aaaaattctt agagaaaatg gtat
//
LOCUS       DAHHPA010000061        16958 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.062, whole genome shotgun sequence.
ACCESSION   DAHHPA010000061 DAHHPA010000000
VERSION     DAHHPA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.062"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            31..1152
                     /locus_tag="NBH55_001856"
     CDS             31..1152
                     /locus_tag="NBH55_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCD1509838.1"
                     /translation="MIVPEGTVANVGDVLVEIDAPGHNSASSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="NBH55_001857"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="NBH55_001857"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD1509839.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="NBH55_001858"
     CDS             2728..3003
                     /locus_tag="NBH55_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCD1509840.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="NBH55_001859"
     CDS             3009..3776
                     /locus_tag="NBH55_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCD1509841.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="NBH55_001860"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="NBH55_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCD1509842.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="NBH55_001861"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="NBH55_001861"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCD1509843.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="NBH55_001862"
     CDS             7920..8222
                     /locus_tag="NBH55_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCD1509844.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="NBH55_001863"
     CDS             8225..9388
                     /locus_tag="NBH55_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCD1509845.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="NBH55_001864"
     CDS             9414..12842
                     /locus_tag="NBH55_001864"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCD1509846.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="NBH55_001865"
     CDS             12863..13987
                     /locus_tag="NBH55_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509847.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="NBH55_001866"
     CDS             13977..14414
                     /locus_tag="NBH55_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCD1509848.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="NBH55_001867"
     CDS             14398..14736
                     /locus_tag="NBH55_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCD1509849.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="NBH55_001868"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="NBH55_001868"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCD1509850.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="NBH55_001869"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="NBH55_001869"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCD1509851.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="NBH55_001870"
     CDS             15893..16936
                     /locus_tag="NBH55_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCD1509852.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5746 a         2931 c         3744 g         4537 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcatc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggt
//
LOCUS       DAHHPA010000062        16325 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.063, whole genome shotgun sequence.
ACCESSION   DAHHPA010000062 DAHHPA010000000
VERSION     DAHHPA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.063"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            72..1499
                     /locus_tag="NBH55_001871"
     CDS             72..1499
                     /locus_tag="NBH55_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1509853.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1591..1956)
                     /locus_tag="NBH55_001872"
     CDS             complement(1591..1956)
                     /locus_tag="NBH55_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCD1509854.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1953..2318)
                     /locus_tag="NBH55_001873"
     CDS             complement(1953..2318)
                     /locus_tag="NBH55_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCD1509855.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2510..2758)
                     /locus_tag="NBH55_001874"
     CDS             complement(2510..2758)
                     /locus_tag="NBH55_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509856.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2757..2885
                     /locus_tag="NBH55_001875"
     CDS             2757..2885
                     /locus_tag="NBH55_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCD1509857.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2942..4636
                     /locus_tag="NBH55_001876"
     CDS             2942..4636
                     /locus_tag="NBH55_001876"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCD1509858.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4640..4759
                     /locus_tag="NBH55_001877"
                     /pseudo
     CDS             4640..4759
                     /locus_tag="NBH55_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4736..4909
                     /locus_tag="NBH55_001878"
     CDS             4736..4909
                     /locus_tag="NBH55_001878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509859.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5287..6420
                     /locus_tag="NBH55_001879"
     CDS             5287..6420
                     /locus_tag="NBH55_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCD1509860.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEKVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6451..6771
                     /locus_tag="NBH55_001880"
     CDS             6451..6771
                     /locus_tag="NBH55_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCD1509861.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6800..7264)
                     /locus_tag="NBH55_001881"
     CDS             complement(6800..7264)
                     /locus_tag="NBH55_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1509862.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7396..8649)
                     /locus_tag="NBH55_001882"
     CDS             complement(7396..8649)
                     /locus_tag="NBH55_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1509863.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8678..8995)
                     /locus_tag="NBH55_001883"
     CDS             complement(8678..8995)
                     /locus_tag="NBH55_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD1509864.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9021..9341)
                     /locus_tag="NBH55_001884"
     CDS             complement(9021..9341)
                     /locus_tag="NBH55_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1509865.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9805..11220)
                     /locus_tag="NBH55_001885"
     CDS             complement(9805..11220)
                     /locus_tag="NBH55_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD1509866.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYHEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11237..12553)
                     /locus_tag="NBH55_001886"
     CDS             complement(11237..12553)
                     /locus_tag="NBH55_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1509867.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12703..13401)
                     /locus_tag="NBH55_001887"
     CDS             complement(12703..13401)
                     /locus_tag="NBH55_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCD1509868.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13690..14646)
                     /locus_tag="NBH55_001888"
     CDS             complement(13690..14646)
                     /locus_tag="NBH55_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1509869.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14759..15592)
                     /gene="msrA"
                     /locus_tag="NBH55_001889"
     CDS             complement(14759..15592)
                     /gene="msrA"
                     /locus_tag="NBH55_001889"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1509870.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15767..>16325)
                     /locus_tag="NBH55_001890"
     CDS             complement(15767..>16325)
                     /locus_tag="NBH55_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509871.1"
                     /translation="KNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIV
                     VNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIY
                     RVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTV
                     TTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5086 a         3284 c         2769 g         5186 t
ORIGIN      
        1 tgatttcaaa aagaaaacat gtcataatat ttcccatatt gtgcaaaaaa atacaaggcg
       61 gaggctctac tatgattgaa gaatatattg agaaaaatat tttaagacag ctgtttttat
      121 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
      181 tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt gaagaacaga
      241 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      301 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      361 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      421 gcaaagctta ttcattaaaa aaacaagtat tagcttactt taatgactgc gggatcgaaa
      481 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      541 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      601 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      661 aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      721 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      781 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      841 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      901 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      961 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt gcgctgattc
     1021 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttt
     1081 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1141 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1201 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1261 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1321 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1381 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1441 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1501 gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1561 aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta ggggtccgat
     1621 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1681 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1741 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgcttactcg
     1801 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1861 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcgaa
     1921 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1981 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     2041 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     2101 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2161 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2221 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2281 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2341 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2401 tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt ctccctccat
     2461 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2521 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2581 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2641 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2701 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2761 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2821 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2881 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2941 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     3001 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     3061 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3121 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3181 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3241 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3301 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3361 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3421 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3481 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3541 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3601 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3661 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3721 agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag aagaagaata
     3781 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3841 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3901 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3961 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     4021 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     4081 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctattg tcgtgcatca
     4141 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4201 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4261 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4321 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4381 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4441 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4501 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta ttcctatgtc
     4561 ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4621 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4681 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4741 agcttttgaa caccgtttac caagaggtta tgaaaaagct gccctgcatc aagtaataag
     4801 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat
     4861 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg
     4921 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4981 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     5041 tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc
     5101 aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga
     5161 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5221 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5281 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5341 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5401 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5461 ggacacgaac caatgggaat cgttgaaaaa gtgggatctc aagttcaaac attgaaaaaa
     5521 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5581 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5641 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5701 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5761 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5821 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5881 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5941 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     6001 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     6061 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct
     6121 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6181 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6241 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa
     6301 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6361 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6421 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6481 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6541 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6601 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6661 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6721 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc
     6781 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     6841 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     6901 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     6961 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     7021 gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga
     7081 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     7141 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     7201 aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     7261 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     7321 aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat cacttgttct
     7381 ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga tcgtagctac
     7441 cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc
     7501 acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta cccatggagg
     7561 cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa cactaactaa
     7621 gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga taggtaaccc
     7681 aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt ttgttacaac
     7741 catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc
     7801 acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc
     7861 gctatgtcca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg
     7921 catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat
     7981 aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac
     8041 cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac
     8101 taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac
     8161 tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac
     8221 aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat
     8281 acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc
     8341 gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag
     8401 aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa
     8461 aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa
     8521 tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg
     8581 ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa
     8641 tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca
     8701 gatcaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac
     8761 tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct
     8821 ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg
     8881 ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat
     8941 ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt
     9001 gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt
     9061 tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt
     9121 gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg
     9181 tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct
     9241 gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc
     9301 attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct
     9361 atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg
     9421 atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac
     9481 taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg
     9541 acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt
     9601 aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat tcttattact
     9661 tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg
     9721 gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa
     9781 tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct
     9841 cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc
     9901 gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca
     9961 gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat
    10021 caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt
    10081 ctcatccata tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg
    10141 gatattatga taattttctt ttagatacat ggctatatca tacatgattt tcggatagat
    10201 ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt catgatatcg
    10261 ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg
    10321 ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc
    10381 tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta
    10441 gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc
    10501 agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca
    10561 gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa
    10621 aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac
    10681 agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt
    10741 taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac
    10801 ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc
    10861 caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg
    10921 ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt
    10981 gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta
    11041 ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc
    11101 atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc
    11161 cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat
    11221 gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg
    11281 gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat
    11341 ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa
    11401 ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag
    11461 gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa
    11521 ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat
    11581 cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc
    11641 ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag
    11701 cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct
    11761 tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata
    11821 gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa
    11881 tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac
    11941 ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca
    12001 cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa
    12061 tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag
    12121 ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa acagttggat
    12181 taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc actttattca
    12241 gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga
    12301 catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct
    12361 gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg
    12421 taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat
    12481 tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc
    12541 cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc
    12601 gcttacattt cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa
    12661 attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc
    12721 tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa
    12781 cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt
    12841 ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat
    12901 gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa
    12961 atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg
    13021 cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc
    13081 catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc
    13141 ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt
    13201 ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg
    13261 caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc
    13321 tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac
    13381 tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa
    13441 ttattataac acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt
    13501 catagacata caaacgaaga agatatttac cataaaaaag actaggataa aattcactcc
    13561 tagccttcga tcatttttat tttgaacgac aaaaaatatg atgttcaata acagacgatc
    13621 ttcattcgat tcttgatgtc atcttttgtt ttttttaata tatggataaa aagtttgatt
    13681 acatcgtttc tattctagaa tttggtcgat tctcaattct tcttctggtg agaaatctaa
    13741 attatccatc attcgaacac tttcttgtaa ctgacttaac cgacttgctc cgaccagtac
    13801 acttgtaaca gatttttgtc gcaaattcca tgcaagggcc atttgcgcta atgattgtcc
    13861 tcgtgatacg gcgattgttt gcagtgcttt gattttttcc agagttgatc caacttgttc
    13921 ttctgaaagg aatggtattt ccttacgatg tgctctagaa tcttcaggaa taccatgtag
    13981 ataacgattt gtcagcaacc cttgagcaag cggactgaac gtgattgccc ctagcccttc
    14041 ttcttctaat actggagata gatcgtcttc tagtagtgca cgagaaaaca tattgtagcg
    14101 catctgatga ataatgaacg gtgcttcctt tctttttaaa atttcagtca ttttttttgt
    14161 atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc ttacaagttg
    14221 catcaaagct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat gatgatagaa
    14281 gatgtcaaca taatctagcc ccattctttg gagactttgg tcacaacttg aaatgatgga
    14341 tttacgagag ccccattccc catatggccc ttcccacata tgataacctg ctttgctaga
    14401 aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc caaagttttt
    14461 ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg taattcccat
    14521 gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat caccaaaatt
    14581 ttgccataat ccgagagata aaatcggtaa tttcaaacct gaatgaccgg atttacgata
    14641 ttccattttt tcataacgtg cactatctgc aatatacaat ttttctcctc ctcatcaaat
    14701 ctttttaaaa cgctttcttt tttctatttt tagtttacta cattttaaag aaaaggagtt
    14761 aatcaaaatg attttttcgt atattttttt gccatttgtt ttttactcgt tgatagatca
    14821 gaaattgatt acgtgcttgc tggatttttt tataacgttt cggttgtttt ttgtaaaatt
    14881 gctgatgata gttttctgct ggccaaaacg taccggctgg ttcgattttt gtgacgattg
    14941 gttggtggaa agttcctgaa ctgtctagtt tctgttttga ttgttccgct aattcttttt
    15001 gtcgttcgac ttgataaaaa atcactggac gatattgctt ccctctatct tgaaattgtc
    15061 caaatgtatc tgtgggatca gtaatttgcc aatagattgt cagcaactcg ctataactta
    15121 tttctcttgt gtcataaatg atctccacag cttccacatg acctgtgtaa ccaccactta
    15181 cttgatcata atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa acagatagga
    15241 tccctttttt cgattcaaac ggctcgacca tacaccagaa acatccccct gcaaatactg
    15301 cacgttcttg atagtcagca tcttgcatgg catgcttgga aaagtcatat ttttgctcct
    15361 tatcaaatcc ttccgtaatc aataaataaa agtccattac atcaggcgtc agattcattc
    15421 ggatagctaa tggacgtagt gttgcttcta gttttgataa ctgttcttta acggatatat
    15481 tttctcttat gtcattcttt gtctgaatca atacttttcg ttcccaatct cgtgtattcg
    15541 gattcaaaat cagattataa agtgtttgaa tgaatatttg ttcctcatcc atagattctc
    15601 ctcctttccg aagaagacaa gctagtgact ttagctgtaa tttatcataa agtgaggaaa
    15661 taagcgatta cttcatcccc ctacagattt ttcgtataaa aaagagagaa tccttatcga
    15721 cagattctcc tttttaatca atttatttta taaatgaaaa gttaatttac cttttatgga
    15781 aaactatttt aaaaaataaa cataaatatg cagttaatgt aacaagataa gaaataatag
    15841 tcgttactgt ataacttgtg cttactttaa tatcagcaac tcctttgact ccgcttattt
    15901 gtaccaatcc attagcattc acctgctcct ttacaggttg atgatttatt tctacattcg
    15961 ttcctaaata agcatataca ggaagtgtga ttacttggct cttctcatta gttactctat
    16021 atatgataga actaccagtt gttttataag gaaggtttac ccattgactg ccaatctttg
    16081 cttcgtgttt tattatatgc tccttattct ccaatgaagc gataggagaa taatcatata
    16141 aaaaataatc cattccttta gtggtatacg cctctactgt ttctttgttt acaacaatat
    16201 gtcccggctg agaaattatt ttttgattga aatttttttt actagaaaca gttaaagtga
    16261 ttaataacaa aacagtcata aataaataaa atagttctct tgtcttattg tatcttctat
    16321 ttttg
//
LOCUS       DAHHPA010000063        16257 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.064, whole genome shotgun sequence.
ACCESSION   DAHHPA010000063 DAHHPA010000000
VERSION     DAHHPA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.064"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..135
                     /locus_tag="NBH55_001891"
     CDS             <1..135
                     /locus_tag="NBH55_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD1509872.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="NBH55_001892"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="NBH55_001892"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCD1509873.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQLTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2411..2662)
                     /locus_tag="NBH55_001893"
     CDS             complement(2411..2662)
                     /locus_tag="NBH55_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCD1509874.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2808..3431
                     /gene="lexA"
                     /locus_tag="NBH55_001894"
     CDS             2808..3431
                     /gene="lexA"
                     /locus_tag="NBH55_001894"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCD1509875.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3611..3829
                     /locus_tag="NBH55_001895"
     CDS             3611..3829
                     /locus_tag="NBH55_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509876.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4118..4630)
                     /locus_tag="NBH55_001896"
     CDS             complement(4118..4630)
                     /locus_tag="NBH55_001896"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCD1509877.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4644..6941)
                     /gene="recJ"
                     /locus_tag="NBH55_001897"
     CDS             complement(4644..6941)
                     /gene="recJ"
                     /locus_tag="NBH55_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCD1509878.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7083..7526)
                     /locus_tag="NBH55_001898"
     CDS             complement(7083..7526)
                     /locus_tag="NBH55_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCD1509879.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7527..8315)
                     /locus_tag="NBH55_001899"
     CDS             complement(7527..8315)
                     /locus_tag="NBH55_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1509880.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8312..9253)
                     /locus_tag="NBH55_001900"
     CDS             complement(8312..9253)
                     /locus_tag="NBH55_001900"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCD1509881.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKRLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9430..10017)
                     /locus_tag="NBH55_001901"
     CDS             complement(9430..10017)
                     /locus_tag="NBH55_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCD1509882.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10238..11545)
                     /gene="obgE"
                     /locus_tag="NBH55_001902"
     CDS             complement(10238..11545)
                     /gene="obgE"
                     /locus_tag="NBH55_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCD1509883.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11839..12849
                     /gene="gap"
                     /locus_tag="NBH55_001903"
     CDS             11839..12849
                     /gene="gap"
                     /locus_tag="NBH55_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD1509884.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12934..13869)
                     /locus_tag="NBH55_001904"
     CDS             complement(12934..13869)
                     /locus_tag="NBH55_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1509885.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14055..15014
                     /locus_tag="NBH55_001905"
     CDS             14055..15014
                     /locus_tag="NBH55_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HCD1509886.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(15100..16194)
                     /locus_tag="NBH55_001906"
     CDS             complement(15100..16194)
                     /locus_tag="NBH55_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCD1509887.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         4613 a         3581 c         2835 g         5228 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcaatt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 tacctttatt tcttacactt tcattctata agatgtcaca cgaaaaagca atctttttca
     2401 gtatttttta ctaaaagttg tcatttcggt tgtgtaagcc ttttttcttt tggatttctt
     2461 ttaatttttc cggggtcacg tcattgccct caggatcgac gactttcatt ccttcgatat
     2521 gatgacgcat accggaacga aaagcttcca aatactcttt gcgcaagttg atttgttctt
     2581 gttcttcggc ttttgagaga ccattttcct tctttttacg tgcaagttca ttgatccgct
     2641 ctattttttc aggtgacagc atgctcagcc ctccttttac tctccttcat attatataaa
     2701 aactgaaaaa aaacaactag ataaactaaa taaaatacga acattcgttt gatttagcgc
     2761 tttttatatg gtactattta tttatcaagt gaaagaaggt gtaccttgtg gcccgacaaa
     2821 ctgaatcaag acaaatcgaa gtcttacgat atatccatga acaagtaacc caaaaaggat
     2881 acccgccgac tgtccgtgaa atcggggaag ctgttcagct ttcttctact tctactgttc
     2941 acggtcatct tgcacggctt gaaaaaaaag ggtttatcca aagagatccg acgaaacctc
     3001 gtgcaatcga gttgacaaaa caaggtttag aaaagattgg gattcgtcca acaactattc
     3061 cagtacttgg cgtcgtaaca gctggagaac ccattttagc agtagaggat gcttcagact
     3121 tctttccaat ccctcctaat ctttcttccg aagaaggcag tctctttatg ctgacaattc
     3181 gtggagatag tatgatcaat gctgggatat tggatggtga caacgtgatc gttcggaagc
     3241 aagaaactgc tcaaaatggc gatattgtca ttgcaatgac tgccgaggac gaagcgactt
     3301 gcaaacgatt ctataaagaa aaaaactttt tccgtctgca gcctgaaaac gacttgctcg
     3361 aaccaatcat cttagatcaa gtatctatat taggacgagt agtaggactt tatcgtagtc
     3421 atatctatta attgctacta attaaaagaa aactttttaa aaagagtttg agacgttggt
     3481 ctcaaactct tttttcgccc gggttcttct tctttcaaat ttttatcact tccctcttta
     3541 caaaccgaac aaatattcgt atactaaata tacgaacaat tgttcgcata aattaaagag
     3601 gtgagaattc atgaaatcaa tgagacgtct tggcttagta ttaactgttt tgtttttagg
     3661 tattattatt ggtaatttcg gcatacgtcc agctttactt gtgtttgttc cgttatttac
     3721 tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa tctgagcggc gcagaattca
     3781 tgaagatcat ttctatcatg agccctacta cagatccagg caatcataat aaggattgac
     3841 tgctaattat tcaaaagaca agaagttaaa cttttacggg ggtgtgacaa aaatcggtca
     3901 catccttttt ttcgaataaa cggtgttcta aagccagttt ttcggtatta agtcaaattg
     3961 cggaaaaagt gattttttaa acttctactg gtctttgctt gtttccattt ataaaaaaat
     4021 agcgaccctc aaaagtcaga tagagaatct gacttttgag ggtcgcttta aaatcgcttc
     4081 tttaagaata acatcaagaa tagcttcttt tttctgctta atattccatt aatgtaatga
     4141 tgtcatatcc tttgattttg tcgcgtccat ggagatccat caattcgatc aaaaatgcgc
     4201 agcctacgac tacaccgcct aattgttcga tcagttcgat cgtcgctttg attgttcctc
     4261 cagtagctag caaatcgtca caaatcagca cacgttgacc tggtttaatg gcatccttat
     4321 gcaatgtcaa tgtatctgtt ccatattcta atccataagt cacttcgatg gtttctcgag
     4381 gtaacttccc ttttttgcgg actggggcaa aaccgactcc taactcataa gcaacaggac
     4441 atcctacgat gaatccacgg gcttctggcc caactaccat atcaattcgt ttttcttttg
     4501 cataatttac gatttgttta gtagcttccc gataagcgtc accatctgcc atcagtgggg
     4561 aaatatctcg aaacacaatt ccttttgacg gatagtcggg aatactggca atataatctt
     4621 ttaaatccac tctttgtttc ctcctaggtt gttaaccatt tctttatggt aggaatatcg
     4681 ctcaaaagca aaaattcctc aactttgatt ttattgagcc gtcgctggta taatctgctc
     4741 tcagtcaaag gatgactttc tgggctagcg actttttgta aaacaccgtt ctctattgta
     4801 acaaatttca attcaaaaaa cacctgtatc ataaaaatta ataatttttc agggatacgc
     4861 aaatattgag ctattgcttt tattttatga cgtatatcaa ttcgttcttg agaatggatc
     4921 aatttaaaca attttgcgta ctgttctctt gtgcctaccc cattcaaata ggcctcatca
     4981 ggtgaaatcc ccatcagata aacacgattg aatgaataag accgaaaaac ttctttcaga
     5041 tccgctaaat catctggaca atccataaca actagagagt ttggttttct tttttcaatt
     5101 attgccttta cagaatctag tccttcgaaa acaatcgcag cttcctggat aatctcaggc
     5161 acgaatttca acgtcgaaga atcaaatgct aaataaaggc taccttccga cgttcggctc
     5221 tgttcacgga aacgttttgc ccgccaatca aatacttgca gaccacccac agaaaaatca
     5281 gataccatca gctgcggttt cttacgcccg ttccattcat tgatcgaaag ctttcctacc
     5341 acatctacct gattattcag cagttcttct tcttgagtac caaaaccaaa agcgaccgca
     5401 tcaagctgac tcgatgcatc ggaaaggaca aatttcagat gctgttggtt tgctccgatt
     5461 cttttgatat tttctgcttg gacttgtcga aataaaaact gaggcaaagg attatccgta
     5521 ccaaatggcg ccaatagttt cagctcatca attcgctcta ctgttacttc atttggcagc
     5581 aatacttcat caattctcaa ctcaggccct ctcatcaagt caatttgatg atcgactata
     5641 tattggttca tcttctcctg tagtatagtg acattttcac ttggcatcgt taaaccgact
     5701 gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca tctggtcaag catttcaaaa
     5761 agattcaagg cttcaatact acgtccagat cctttagctg atccatcttc ttttaaagta
     5821 aggacaagcg ttggtttacc cgtttcattc attattttcc cagcaacaat gcccaaaacg
     5881 ccttcatgcc atcctcggtt taccaataga tggatatgat tctccttatt gaccatcgct
     5941 aatgcttctt gtgttatctg ctctacgatt gcttttcttt cttcattgat ctcattcaat
     6001 ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg caagtaacga gacggcagga
     6061 cgtggatcgc ccatccgacc gattgcattt agtctgggag caatcgaaaa accgatcgtt
     6121 gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa atagcgcctg aagtcctatc
     6181 cgttctgaat gatgaatggc atcgattcct aatgaaacaa ggattcgatt ctcatcagtc
     6241 aatgaaacta aatcggcgat cgttcctatc gctactaaat ccaaaaattc tgcaggcggt
     6301 tcttccaata atgcagaagc tactttgaat gctactccga ctcctgccaa atctggaaat
     6361 ggatagtgcc ccgctggatg acgagggtga acaatcgcat aagcgtctgg taaattctca
     6421 ggtagttcgt gatgatctgt aatgatcacg tctacaccag cttgctgagc gtatgctacc
     6481 gcatcatttc ctgctacccc gttatcaaca gtgatgatca actggattcc ttcttcgatc
     6541 ttttcttggt ataccgattg gtttggtcca tacccatgct caaatcgatt tggcaggaat
     6601 acttctactt ctgcaccaag taattccagt gtttctttca ttaccgttgc actggtgatc
     6661 ccatctgcgt cataatcacc gtaaacgagg attttttctc cactgattac agcttcttgg
     6721 attcggctga ttgctttatc catatcgtgt atcagatacg gatcatgtaa ttcttgttcc
     6781 tttggatgta aaaatcggtt gatatccttt tcctcatggt aacctctttg ccacagtaac
     6841 ttaccgacaa atggagataa tttcagttca cggatcattt ggagaaagga ttcagaaggc
     6901 tctgtctcgc ttaacttcca atcaaaattt gcttgtctca ctttctcact cctaacttag
     6961 ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga aagaataaaa acagatcaga
     7021 tccgctagac agatgttcag caggacctga tcttttgtac gaccatttat gaaatatccc
     7081 tattagtcaa aataattgaa gctttgtttg ggcgattgag ttttttgttc ttccggcgct
     7141 ttcaattgac tgatttgtgc ggttttcgct tgtaactccg cctgataagc agcttccaac
     7201 tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt ttgcttcttc aattttcttt
     7261 tgaagttctg tttgatattc tgacatttcc tgagtcagct gtttgatttg ctttttttgc
     7321 tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg caccaatcag cgcagaaccg
     7381 atgatcacta agatcaatgg cgcagaaaag gaagtgaagc caaaacttac aggaacattt
     7441 ttattgttca atacagcaaa aagaacaata atcaaaacta aaacaaatcc aagaatcaca
     7501 ttgctttgct ttttcaattc cttcacctac tttttgttaa aaattccgct ggctaaataa
     7561 tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa cttctaaaat aaacggttga
     7621 ttgatttccc gttttggatg tctcatattg cggacgatca tttttgccaa cttggcaggt
     7681 tctaaaacga tttgtccgac tttttccaaa tagcttccgc ttggatcagc cttatcgaag
     7741 aaatttgtct cgattggacc tggattcact gtcgtaacag ctacgccaag tggtttcaat
     7801 tctaatcgca aagcgttgga gaagccaagg actgcaaatt tcgtggcaga ataaaccgtt
     7861 gattttgcag ttgccatttt tccagccata gaggcgatat taatgatatg accttgtctt
     7921 ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca tcattcctaa gatgttgact
     7981 tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga agtcttcgaa aatcccaaaa
     8041 cccgcattgt ttaccagaat atcgacgcgc ccgacttctt ccatcacctt ttcatataat
     8101 tcgtccacgc tttcaggatt gctgacgtct accttgaaag cataagcctc tttgccgctc
     8161 aagcgtctgc actcgtctct gacaccttct acaaatgcca ttcttcgtgc gcaacttata
     8221 acgattgctc cttgctttgc tgcttcataa cagatttgtg cgcccagacc gcctgaacta
     8281 cctgttacta aaacaacttt atcttctaaa ttcatgcctc ttcctcctcg cgaaatggaa
     8341 tctcaatgct atcaaaatct ttcataatcg tcgtgttttc aaacacttct tgtgcttctt
     8401 tttcaagctc taaagctgct tttcctaaat atctcgcact gatatgagta agtagcagcc
     8461 gttttgcctg cgcttctttt gcaacttcag cagcttgatg agtcgtggaa tggaaatagt
     8521 tgtgcgccat tcttgcttca tctttattaa atgtgctttc atgtaccaga atatcgctat
     8581 tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc gcctaaaata gtcacgattc
     8641 gtcctttttt atctggacct acgaaatctt ttccattgat cgtccgccca tcttctagtt
     8701 ggatcgtttc accttgcttc agtttgccgt acagtggtcc agctggaata cctaattctt
     8761 ttagacgatc cacctgcaat tctcctttat gatcatgttc aaccacacga taaccaaaac
     8821 tgtcgatccc atgatttagt ctttttgtat aaacagaaaa ttgctgatca gtaaaaatcg
     8881 gggctgtttc atttagctca atgaatttca gcggataact taaacgcgtt tgcgaaatac
     8941 ctaaagagac ttttatgtat tcctctatcc cttttggccc gtaaatctct aatggcgtat
     9001 ccccaccttg aaatgaccgg ctgctgatga gcccgggaag tccaaaaata tgatcgccgt
     9061 gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg aatagtcgtt cgcaaaatct
     9121 gcatttgcgt gccttcgcca cagtcgaaca accaaacttc attgcgctcg tctaaaagtt
     9181 tcaatgctat acttgtcaca tttctatgtt ttgcaggaac acctgcacct gttcctaaaa
     9241 attgtaattc cataatctac ctgactttct ttaagttcct ctttaatttt agggtaaaaa
     9301 gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt aaaaataacg ttctaaagcg
     9361 atagcgtttt ctttgaaaat tactataaaa acagcagcga acccgtttga gatcctctgc
     9421 tgttttttat taatttgaag atccagaact ttcattgtcg ttggaagttt gcggatgttt
     9481 gtccttccaa gtcttttgtg cagcttcttt cgcatgattt tcagcttctt tcgcatcatg
     9541 cacatctttt tcgacatctt cataatccaa gcgagtaatt tctccgccta attccatcat
     9601 taccaattga ttcagtgggc gattgtcctc ttcatcagca atcaagatca gtcctgtagc
     9661 tccttcaccg attttatttc ctacaaagtc gaacacggtc tgggcatctt ttatttcttt
     9721 ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat cctaataaaa tccctagcgg
     9781 accacctaat acaccaacca acattccgat taatccaccc gtagaagatt tgttattacc
     9841 ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc ccattaggat catggctgac
     9901 aactgccatc tgttctcctt taatcgaacg aacagcatgt aactttttga tttctgaaaa
     9961 tgcctgatat gcctggcttt ctacttgaaa atttaaaatg attactcgtt tgctcattaa
    10021 aaacgcccct ttcatacctt cttgttattt attttacctt taattgaaaa ataagaaaag
    10081 aaaagcactc tttttttcca ataaaatagg gctgtgacaa aagtcgtatc acagctcctt
    10141 tttccgaata aacggcgttc ttgaagctga tcacttctgt cacaacctcc cttaaatctg
    10201 aatctatatt ttttggtatt tgtttttatt tgcttgccta ttcgacaaat tcaaattcaa
    10261 actcaccgat acgtacaagg tcgccatctt tcgcaccgcg tgcccgaaga gcttcatcca
    10321 cacccatacc acgtaattga cgagcaaaac gcatcactgt ttcatcatga tcgaaattcg
    10381 tcatttggaa taatttttcg atcttctcac cagataagat ccacgttgca tctgaatcac
    10441 gatcgatttg gaactcagga ccttctggat tgaatccata gtgtacagtt tcttcttcca
    10501 gttcttcttc gtacaatggg aattctggcg ttacttctaa caaatctgct gtagcattca
    10561 ataacgcgtc caatccttgt cgagtcactc cagaaattgg aaatactggg atgtcatcag
    10621 caaattcatc ttctttttcc ttgttcagct gttctttgaa tttcactaga ttttccggtg
    10681 catccggcat atccattttg tttgccacga tgatttgcgg acgctctagc aagcgtaagt
    10741 tataagtaga taattctttg ttgatggcaa gatagtcttc ataaggatct cgtccttcca
    10801 tcccactcat atcgatcaca tgtaaaatta ctcgtgttct ttcgatatga cgaaggaatt
    10861 gtgtacccag cccaacacct tgcgaagctc cttcaatcaa gcctggcaag tctgctgcgg
    10921 caaaacttct gccatcactt gtagtaacca ttcctagatt aggtacgagc gtcgtgaaat
    10981 gataagcgcc gatttttgga cgagcagatg aaataacgga tagtaaagtc gatttcccga
    11041 cagatggaaa acctacaagt ccaacgtctg ccaatacttt caattctagt tcgatttttc
    11101 gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc aggattccga ggagaggcaa
    11161 aacggatatt ccctcgtcca ccgcgtccac cttttgccac aactagtgtt tgaccatttt
    11221 caattaaatc tcctaaaaga gcaccagtct ccgcatctcg gacagtggtt ccttgtggta
    11281 ctttaacata ggtatcttct gaaccacgac cgtgcatccc tttactcatc ccattttcac
    11341 ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat caatgtacgt aacccttcat
    11401 cgacgatcaa gatgacatcg ccgccacgac caccgtctcc gccagccggt ccgccgtcag
    11461 gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc gcccttgcca gctttcacat
    11521 cgatcgttac ttgatctaaa aacatggaca taattaagtc ctcctattat tcatttattt
    11581 atttgactgc ctgaatttcc acaataaaca aaagtcttac ttattttatc ggtgaaatct
    11641 gattttgtct atcatttcag caaaaagatt gtttaccttc ttcttttttt atcatttggt
    11701 aaggtttcca accttttttc tgctaccaag tataattttt tgacaaaata ttctttgcgt
    11761 agcagaataa cagtgtatta attgtattaa ttgaggatgc cgtttcacag cacccctaat
    11821 aaaggaggaa cttttgatat gacagtaaaa gtaggtatta acggatttgg gcgtattggc
    11881 cgtctggctt tccgccgcat caaagaagtc tcagatgata ttgaagtagt agcaatcaac
    11941 gacttaacaa gtccaactat gttagcacaa ttattgcaat ttgactctac tcatggtact
    12001 tatcctggca cagttactgc aacagaagac tcaatcgtag tggatggcga agcaactcgt
    12061 gtttatgcag aacccgacgc aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc
    12121 gtactagaat gtactggttt ttatacatct gaagaaaaag caaaagcgca tttagatgct
    12181 ggtgtcaaac gagtagttat ttctgcccca gctggtgcta tgaaaaccat cgtctataac
    12241 gtcaatgatg atacattaga tgaaaacgac aaaatcatct cagcaggttc atgtacaaca
    12301 aactgtttag cacctatggc ttatttctta aataatgaat ttgggatcga agttggtaca
    12361 atgacaacag ttcatgccta cacttccaca caaatgctct tagatggacc tgtaaaaggc
    12421 ggtaatttgc gtgctgctcg ttcagctgct gacaatacta tcccacattc tactggtgct
    12481 gcgaaagcaa tcggcttagt tatccctgag ctacaaggaa aattacaagg acacgcacaa
    12541 cgtgttccag tcgttgatgg ttcattaact gaattggttt cagtattgaa aacaaaagta
    12601 actgctgatc aagtaaacga agcaatcaaa aaacacacag ttgacaatcc ttcattcggg
    12661 tatgatgacc gtcaaatcgt ttctagcgat gtcattggaa caactcaagg ttcgatcttt
    12721 gacccaacgc aaacagaagt aacaactgct ggtgatttcc aacttgttaa aactgttgca
    12781 tggtatgaca atgagtatgg atttacttgc caaatgattc gcttactaga aaaattcgct
    12841 aacttgtaat ttgatagatc aatcgataaa agaagccttt gactggatcc agtcaaaggc
    12901 ttctttcttt tcatccccta cttttaaact cgttcaatga acatattggc ttaaaaattg
    12961 ttggactact tgctgatatt tttcaggatc tgtcttgtat gcttccgcat gctcggctcc
    13021 ttttacaaca tacttttctt ttggtccctt cgttgcacga tagaccttat ctaacatttc
    13081 aaatggtaca aatgtatctt cgtctccatg tataaagagc attgggcgct ggtttttctt
    13141 caattgttcc actgcatttg cttcaccgaa aaagtagcca gcacggactt ttgtcactag
    13201 actcgttact tgcataagcg gaaatgctgg aagactaaac atatcgtcta gctgataagc
    13261 aagctcatca tgaacggaag aataaccgca atcttcaatg attgctttca cattatctgg
    13321 tagtttttct ccgctagtca tcataactgt tgctgctccc atgctgacac cgtatagaac
    13381 gatttcttgc gattttccat tgttttctag cactttgttt atccactgta catagtcttt
    13441 acgctctggc catccaaatc caatgtaatc cccttcgctt tttccatgtc ctcttgcatc
    13501 aggaacaagg acattgtaac ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc
    13561 agcgtttccc atatagccat gagccacaag agctgttttt tcacttttcg tttcagcagg
    13621 aagataaatc gctgataatt tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt
    13681 ttcttttgaa ctaaaccatt tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg
    13741 tgtatcttcg tctaaaaagt ctttctcaga aggcacaaca gcataattat aaaaataatt
    13801 ccctgcaaac actaacgcaa caatcagagc taaagcgacg cctaaaagga ttttcatcca
    13861 aagtttcata aatcatccat ctcatttctc ttttttcata taaccttgaa ttagtataat
    13921 caaagaatct ctactatgca atgttcattt ttataacccg cgaattgtaa aattaagtta
    13981 gaaaaataaa aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag
    14041 gagtgtaacc ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat
    14101 catattatct tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac
    14161 aaactattta taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc
    14221 accagtataa agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg
    14281 aagtagatta cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc
    14341 gaaaagaaag gtctgtttca tgcggtatga gaacacttta tcgtttattt tctaaaggaa
    14401 tatttgatat tgacacacta ccgatgaaag gtaaaagaaa acccaatggc catcaggaaa
    14461 aacggggaaa acaacagtat cagcgctcaa tccatgatcg acctgataat tatcctgatt
    14521 tcaattctga gtttggtcac cttgaaggtg atacgatcgt tggcattcat cataaaagtg
    14581 ccgtcattac tttagttgaa agattatcta aagtcattat cacgattaaa cccaacggcc
    14641 gtaaggcatt agatattgaa actgccctta atcaatggtt ttctcgcttc cctaaaaact
    14701 tctttaaatc tattacgttt gactgtggaa aagaattttc taactggaaa gccattagta
    14761 accaacatga tattgatata tattttgcgg accctggaac accttctcaa cgcccattaa
    14821 acgagaattc taacgggatt ctgcgtcgta atggactgcc gaaatcaatg gattttagaa
    14881 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
    14941 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
    15001 ctagcttaat ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact
    15061 ggctatttct agcggattat ttctgcaata actaattatc tattttttat tttcaattaa
    15121 tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct
    15181 tcctacaaca ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc
    15241 tttgtattga ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt
    15301 attatgaggc gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat
    15361 atattctgag cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt
    15421 ttctgtaata ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc
    15481 tgccatttct ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat
    15541 caatcctgta aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc
    15601 cagcgtgcac aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac
    15661 gaatgccatc gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc
    15721 gtatcctaaa ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt
    15781 gtttggcgaa taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc
    15841 gattttgata ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat
    15901 atctaagcgg accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat
    15961 ctccaactgt tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt
    16021 cgcgtaatca actacttctt taaattctga aagaactttt tctttatcat cattgatctg
    16081 cagcaaactg gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt
    16141 gtccttttca aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc
    16201 atttcctcct gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagt
//
LOCUS       DAHHPA010000064        16252 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.065, whole genome shotgun sequence.
ACCESSION   DAHHPA010000064 DAHHPA010000000
VERSION     DAHHPA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.065"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..624)
                     /locus_tag="NBH55_001907"
     CDS             complement(<1..624)
                     /locus_tag="NBH55_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509888.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPET"
     gene            complement(803..1522)
                     /locus_tag="NBH55_001908"
     CDS             complement(803..1522)
                     /locus_tag="NBH55_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1509889.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1653..2804)
                     /locus_tag="NBH55_001909"
     CDS             complement(1653..2804)
                     /locus_tag="NBH55_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCD1509890.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3021..3761)
                     /locus_tag="NBH55_001910"
     CDS             complement(3021..3761)
                     /locus_tag="NBH55_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1509891.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3765..4088)
                     /gene="rsfS"
                     /locus_tag="NBH55_001911"
     CDS             complement(3765..4088)
                     /gene="rsfS"
                     /locus_tag="NBH55_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCD1509892.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4145..4738)
                     /gene="yqeK"
                     /locus_tag="NBH55_001912"
     CDS             complement(4145..4738)
                     /gene="yqeK"
                     /locus_tag="NBH55_001912"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCD1509893.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4722..5372)
                     /locus_tag="NBH55_001913"
     CDS             complement(4722..5372)
                     /locus_tag="NBH55_001913"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCD1509894.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5382..5693)
                     /gene="yhbY"
                     /locus_tag="NBH55_001914"
     CDS             complement(5382..5693)
                     /gene="yhbY"
                     /locus_tag="NBH55_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCD1509895.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5709..6818)
                     /gene="yqeH"
                     /locus_tag="NBH55_001915"
     CDS             complement(5709..6818)
                     /gene="yqeH"
                     /locus_tag="NBH55_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCD1509896.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6815..7342)
                     /locus_tag="NBH55_001916"
     CDS             complement(6815..7342)
                     /locus_tag="NBH55_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCD1509897.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7522..8307)
                     /locus_tag="NBH55_001917"
     CDS             complement(7522..8307)
                     /locus_tag="NBH55_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCD1509898.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8300..9169)
                     /locus_tag="NBH55_001918"
     CDS             complement(8300..9169)
                     /locus_tag="NBH55_001918"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCD1509899.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9170..10549)
                     /locus_tag="NBH55_001919"
     CDS             complement(9170..10549)
                     /locus_tag="NBH55_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCD1509900.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10557..10976)
                     /gene="fabZ"
                     /locus_tag="NBH55_001920"
     CDS             complement(10557..10976)
                     /gene="fabZ"
                     /locus_tag="NBH55_001920"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCD1509901.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11001..11477)
                     /gene="accB"
                     /locus_tag="NBH55_001921"
     CDS             complement(11001..11477)
                     /gene="accB"
                     /locus_tag="NBH55_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCD1509902.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11480..12715)
                     /gene="fabF"
                     /locus_tag="NBH55_001922"
     CDS             complement(11480..12715)
                     /gene="fabF"
                     /locus_tag="NBH55_001922"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCD1509903.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12734..13471)
                     /gene="fabG"
                     /locus_tag="NBH55_001923"
     CDS             complement(12734..13471)
                     /gene="fabG"
                     /locus_tag="NBH55_001923"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCD1509904.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13483..14400)
                     /gene="fabD"
                     /locus_tag="NBH55_001924"
     CDS             complement(13483..14400)
                     /gene="fabD"
                     /locus_tag="NBH55_001924"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCD1509905.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14507..14731)
                     /locus_tag="NBH55_001925"
     CDS             complement(14507..14731)
                     /locus_tag="NBH55_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCD1509906.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14784..15746)
                     /locus_tag="NBH55_001926"
     CDS             complement(14784..15746)
                     /locus_tag="NBH55_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCD1509907.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15756..16196)
                     /locus_tag="NBH55_001927"
     CDS             complement(15756..16196)
                     /locus_tag="NBH55_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1509908.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4278 a         3708 c         2787 g         5479 t
ORIGIN      
        1 tgtttcagga tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc
       61 tggatattct gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg
      121 gtaaattcga ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc
      181 gccacaatcc aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt
      241 ttttttcgtg tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa
      301 gctccactct aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa
      361 ggctgaatca gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat
      421 tggtgcgcgg tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt
      481 tcctaatcga tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc
      541 agttacgttc acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg
      601 ttctaaagtg atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca
      661 tttcacatga tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc
      721 acacaaaaaa gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat
      781 acttttcgtt aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta
      841 catcttctag atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag
      901 atagtgttac ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc
      961 ctttttcttt taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga
     1021 tttgtccttc ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa
     1081 ggatctcgtc agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag
     1141 aaaccgctcc ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg
     1201 ctgctgttcg attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc
     1261 caaatccttc atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga
     1321 ttgctcggtc aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac
     1381 gtagtttttg gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa
     1441 tcccaaattt tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga
     1501 tatttgccca tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg
     1561 caaaaacgtc ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc
     1621 ttccttcatt cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa
     1681 ttctgttccg gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc
     1741 gcttcttgtg cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt
     1801 aagaatcttc ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg
     1861 acaggaatct cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt
     1921 gtccatgtat atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt
     1981 gcagctgctg ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt
     2041 tccagcaaag tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc
     2101 tgcagcgaac aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt
     2161 tttccttctt ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg
     2221 attttttcat gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca
     2281 tacttggcat tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca
     2341 agcgtaatat cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac
     2401 gtttgatcag acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag
     2461 cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca
     2521 cagtccagtg tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc
     2581 gcccattcga cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg
     2641 cgatgccatt tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca
     2701 gcaatgacga cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca
     2761 ttgtgaaaag gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat
     2821 agttatcgat aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc
     2881 cgaactatat aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa
     2941 gagcaatgcc tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt
     3001 aactcgagca ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc
     3061 tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt
     3121 tccagcattc ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca
     3181 cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg
     3241 ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa
     3301 tgatagctat attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa
     3361 aatcggccat tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt
     3421 ctatttttca tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt
     3481 tctgataaat cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct
     3541 cgctggctgg caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca
     3601 tcttttgcaa aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt
     3661 gtgtcttctt gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc
     3721 atcacttcat cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca
     3781 ctaagatcga ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct
     3841 ggcgcatgga aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct
     3901 tcttttcctt caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct
     3961 tctgtaattg cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc
     4021 aacaaggaaa catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct
     4081 gcttcaatcg caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag
     4141 cagcttattt ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt
     4201 gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta
     4261 gatcagccca tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa
     4321 tatagtctgc tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat
     4381 gcacccggat tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg
     4441 aagcgccaac aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac
     4501 cgtctcttac gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg
     4561 tcaatgcagc aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg
     4621 ctgtttcttc tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt
     4681 tttgcatcag gttttctcta gaataagcag tatattcatt actatagtcc atattcatac
     4741 aacccttcat tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag
     4801 ccttctttga tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc
     4861 caaattacag gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg
     4921 accatagaag tcagctcatc gattttatac cattttggca aatattctac catatcccca
     4981 ccgataataa agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca
     5041 taggtgtaac ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg
     5101 tcttccaccg ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca
     5161 tcaacatgag gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg
     5221 ccagcttgtt cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag
     5281 atccctactt gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa
     5341 cgaacaaatg tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct
     5401 tttgatagcc tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca
     5461 atgatttgga cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca
     5521 atctcatctg tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct
     5581 tcaatttgaa cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga
     5641 agatgatgcg ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc
     5701 cttcttcctt aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc
     5761 gcaattacta ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt
     5821 ttttctttca cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga
     5881 cgaggaggct gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt
     5941 ttcgttcgat gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg
     6001 ctgataaaat caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga
     6061 taaactttag gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag
     6121 tagtgcgcca tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca
     6181 tctaaaggaa tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca
     6241 tcttgtactc ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt
     6301 gttactccga cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt
     6361 tccgtcatct catgagattt cttagcacta gttaataata catctactgg acgaattcct
     6421 gcttcgtaag cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt
     6481 aatatatcca ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt
     6541 cctgggatta gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct
     6601 ttttgcccta attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg
     6661 gtctcattat aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa
     6721 cctttttcca aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata
     6781 acggcaccac agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca
     6841 tatcagggtg ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt
     6901 gtgtcttcca actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac
     6961 gattggctcc acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg
     7021 ctaaatccaa ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg
     7081 caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg
     7141 aaacaacaac aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt
     7201 ctgttccatc tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt
     7261 tgatgcctag attctttaat tgggcaggtg taatctgata gatggcttct accatccatg
     7321 ttggtttgta atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa
     7381 agtactcctc aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt
     7441 tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa
     7501 aaatttagaa acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa
     7561 tttatgctga tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat
     7621 gtgtgcgact ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc
     7681 aacgattcct aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc
     7741 ggcgcgacta ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata
     7801 aatcgtttct tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact
     7861 tccaccttca ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag
     7921 atttctggca atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc
     7981 tgctgtattg atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg
     8041 taaagctttt cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg
     8101 cagatcccgt cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat
     8161 tccaccaaca atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga
     8221 atcgaagatc tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg
     8281 aacgacatca tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa
     8341 gaagcgtatg gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat
     8401 gagataataa gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt
     8461 cgatgacgcg tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc
     8521 cttccattgc aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat
     8581 aaaaaagacc ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta
     8641 gagaaaagat tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa
     8701 gactttcctt tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca
     8761 tacttcccat gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg
     8821 ttgctttacc agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt
     8881 tttccgtata ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt
     8941 caaaactttt ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt
     9001 atccgcaatg agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc
     9061 aatttggaca ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct
     9121 ctctattagg attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc
     9181 ctatctccgt ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt
     9241 aatcacctgc tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga
     9301 tgattccttc tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac
     9361 gcgtatttcc atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat
     9421 atcccgggta aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt
     9481 tttggatctt ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc
     9541 attcgattgc gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg
     9601 ctgctatctc cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat
     9661 gttccacttg gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca
     9721 gaaactcgat cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt
     9781 ctcctagtgc ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt
     9841 tttgattgtt gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc
     9901 gatcacctaa gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca
     9961 tatcatcatt tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga
    10021 gctcttcttt tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca
    10081 gcatcactgg ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata
    10141 caccagtact tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga
    10201 tcttatttcc cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact
    10261 cttcacacat ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg
    10321 cttcggcttt tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa
    10381 cggcttttgc cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg
    10441 catctgcttc tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga
    10501 tacgtacagc gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat
    10561 ccaatcataa aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct
    10621 ttgcctattc cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct
    10681 cccggtgtaa ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt
    10741 cccttgaatt gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca
    10801 atcaataccc ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg
    10861 atcgttacat ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca
    10921 atcaaaagca ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt
    10981 ttccctcttt cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg
    11041 acaactgact cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc
    11101 tcattcatca gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca
    11161 cctacttttt tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt
    11221 ggcgaaacaa cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga
    11281 gcagttgttg cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt
    11341 gtttgaacca tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga
    11401 ccttcacgca aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg
    11461 atttcattga tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc
    11521 cgccaaaccc taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt
    11581 tgacaactgt caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa
    11641 attgatgttc taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata
    11701 aatgtccagt catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg
    11761 cgatttggat tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat
    11821 tgatatatcc gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt
    11881 tagatgcacc agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac
    11941 cgtatccaac gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt
    12001 caagaactag aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg
    12061 gaatcgaacc acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag
    12121 caatccccat ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc
    12181 catgtttgat gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta
    12241 cgatagctgt acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt
    12301 tggcaatcgc cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac
    12361 gaataacttg gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa
    12421 accggtcttg attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag
    12481 cgtagattcc atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt
    12541 tatcgtaagg gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa
    12601 atttagtgat cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg
    12661 cgtcatttcc aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt
    12721 tgctctcctt ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg
    12781 tgatatatgg attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga
    12841 aacgctgcat cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg
    12901 tcatatcagt tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag
    12961 cttctcgtgc cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg
    13021 cttgaccaat atttccaatc aacccagata cactagagag attaatgatg gccccttctc
    13081 gctgcttcat catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga
    13141 tatccagaca ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga
    13201 ttccggcatt gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca
    13261 tttgtcccgc tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga
    13321 atgcttcgat ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat
    13381 ttgctccagc ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg
    13441 tgataaatac attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt
    13501 ttcatcaaat gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt
    13561 cataaatccg cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt
    13621 ttttaactta tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag
    13681 tcccgcgatt gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga
    13741 aggttcagag aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa
    13801 aatagctgta tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc
    13861 ttttaatagt aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg
    13921 aggtgtatta taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc
    13981 ctcgatcgtt tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc
    14041 agcttctgtc atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag
    14101 cgcattacta gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg
    14161 gatgattcca tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg
    14221 ctgtgtatat tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc
    14281 gtagccgaga atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa
    14341 atcttttccc ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat
    14401 gtttttctcc aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac
    14461 attcctattt caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt
    14521 tttctacgta agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca
    14581 cgtcaaattc atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta
    14641 agtcttcttg gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg
    14701 cgatgatttc ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct
    14761 ttttattttt ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct
    14821 ccgccatagc ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa
    14881 actgcttcat ccaataagat agggatgctt gcagcagatg tattcccata tttatccata
    14941 ttggtcagga acttttcttg cggcatcttt acttttcggg caattttctc aataattctg
    15001 aggtttgcct gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata
    15061 ttgtttgcaa cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa
    15121 tacgaacctt ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc
    15181 aatgagctac ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga
    15241 ttcgcttcta agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca
    15301 gaccaatcga ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg
    15361 cagcggatca ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg
    15421 atatcaaaag cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat
    15481 ggtgtcgtgt aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt
    15541 agatttttct gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga
    15601 tttgttacac atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta
    15661 tccatcatct tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt
    15721 gcagtaatca ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg
    15781 tgcaagtttt tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag
    15841 atgttcttga ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt
    15901 ttcgttagtc ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag
    15961 ccttttttta ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct
    16021 ttggctactt ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc
    16081 tctgtgattg acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa
    16141 atatcattga agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc
    16201 caccgtccac taattagttt gattatcaaa tcttttgatg ttcaaactat at
//
LOCUS       DAHHPA010000065        15759 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.066, whole genome shotgun sequence.
ACCESSION   DAHHPA010000065 DAHHPA010000000
VERSION     DAHHPA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.066"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..296)
                     /locus_tag="NBH55_001928"
     CDS             complement(<1..296)
                     /locus_tag="NBH55_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509909.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERY"
     gene            complement(516..882)
                     /gene="ssrA"
                     /locus_tag="NBH55_001929"
     tmRNA           complement(516..882)
                     /gene="ssrA"
                     /locus_tag="NBH55_001929"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1053..1826)
                     /locus_tag="NBH55_001930"
     CDS             complement(1053..1826)
                     /locus_tag="NBH55_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1509910.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1970..2320
                     /locus_tag="NBH55_001931"
     CDS             1970..2320
                     /locus_tag="NBH55_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCD1509911.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2301..3017
                     /locus_tag="NBH55_001932"
     CDS             2301..3017
                     /locus_tag="NBH55_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1509912.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3017..4465
                     /locus_tag="NBH55_001933"
     CDS             3017..4465
                     /locus_tag="NBH55_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD1509913.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4535..5044)
                     /locus_tag="NBH55_001934"
     CDS             complement(4535..5044)
                     /locus_tag="NBH55_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509914.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5034..5759)
                     /locus_tag="NBH55_001935"
     CDS             complement(5034..5759)
                     /locus_tag="NBH55_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509915.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5982..8102)
                     /locus_tag="NBH55_001936"
     CDS             complement(5982..8102)
                     /locus_tag="NBH55_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCD1509916.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8332..9510
                     /locus_tag="NBH55_001937"
     CDS             8332..9510
                     /locus_tag="NBH55_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCD1509917.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9526..10284
                     /gene="aroD"
                     /locus_tag="NBH55_001938"
     CDS             9526..10284
                     /gene="aroD"
                     /locus_tag="NBH55_001938"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCD1509918.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10307..10792
                     /gene="ybaK"
                     /locus_tag="NBH55_001939"
     CDS             10307..10792
                     /gene="ybaK"
                     /locus_tag="NBH55_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCD1509919.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10863..12116)
                     /locus_tag="NBH55_001940"
     CDS             complement(10863..12116)
                     /locus_tag="NBH55_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1509920.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVYLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12233..13582
                     /gene="gdhA"
                     /locus_tag="NBH55_001941"
     CDS             12233..13582
                     /gene="gdhA"
                     /locus_tag="NBH55_001941"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCD1509921.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13694..15040
                     /locus_tag="NBH55_001942"
     CDS             13694..15040
                     /locus_tag="NBH55_001942"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCD1509922.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15348..15734)
                     /locus_tag="NBH55_001943"
     CDS             complement(15348..15734)
                     /locus_tag="NBH55_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD1509923.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5158 a         2818 c         3168 g         4615 t
ORIGIN      
        1 ttataccttt ctggatcaac gaaagtatac ttcaaatagt cataacgccg atgatcgctc
       61 cgtgcgtctg gcacatcagt cacgatatca aacaaaaaat acacatcttt cttcattctg
      121 gtttttgttg ccggaatttt aaagtagttc ttattagagt aatagagatt gattaataag
      181 ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa atctttgaca
      241 aatctattcg aaggatcaaa tttaaaccat aattgtgttt ttccttccat caacataatg
      301 ttcacctcaa aaagagtata cgaactaatg ttctttttgt aaagtaggaa tatcattgat
      361 aatatggatg gaacatatat attaaaaagt gttctttttg aatgctattt tgaatgccat
      421 tttcattcat ttaacgaaaa tctgcgaaaa taaaaaatgc tcaaaccctt ataaaataag
      481 gatttaagca tctagcgaaa tctgctgaaa tctcacaatg gagacggcgg gagtcgaacc
      541 cgcgtccaaa cacatcgcca cttaaaaatc tacgttcata gtcaactcat ttaaggattc
      601 actttacagc ttgccgagtg acaggcattc tgtatcgcta gtctgatgtt ctctttttca
      661 acttacagac ggaaagttga aacgtatccc actaaagtta ggacccttac tcgagcacat
      721 gggcgatgcc gagaggatct acgctaactg tttttaggca gctaaagcgt aagagttgtt
      781 ttcgttttta gcagttatat ttaactgtaa cgtttttacg tagacgtaac ctacgaaacg
      841 caattcaagc tcgactgtgc ctgtcgaatc cgtaacgtcc ccgttagcta acaagggtta
      901 cgtgtatttt tcatacaaaa ttagtatagc atatattggg ataaattcaa taaaaaagcg
      961 aatcatatcc cattatttat ataataactg cccctttctt aagaaaaaat aagcgatttt
     1021 cttaagaaaa gaggtagctt caaaaaaaga tcttaaatca gatcaaaata cttcaatgcg
     1081 tggacaattc cgccttctgt atttttcttt gtgataaagg tagaaagttc ttttaatccg
     1141 tcataggcgt tgcccatagc gattttgttg tcgcaagctt ccaacaaggc aaaatcattc
     1201 ggaccatcgc caaatccaaa agtaggaact ttttccagct gaagttcttt ttgcagacgt
     1261 ttgacacctg ccccttttga gattcctttc ttcactgtat cgatcgagta aggaccattc
     1321 cgataaaaag ttagctcagg aaaatgttcg tgatagtatt cgtcacctga ttgagataag
     1381 atcagcaaca tatttactgt gttttcttta aagcctaaag gatcaatttc cggaacatct
     1441 gaatgaatga agtcgtaagc attgatcatt gtttggctat gaccggtaca ccagattttc
     1501 tgatcgttat agtaagaaag ttcatgaccg ttcaactgta catgatcata cattttttga
     1561 cattcttctg aagaaaatgt ttgagaatag atttcttcgc cgtctactcg aatgaaagaa
     1621 ccattcatgg tgaccgcaga agtaatgccc gcatcttcca tgatagagtg gatttctgct
     1681 tctgtacgtc ctgtagcgat gacggggaga acattgtttt cttttaatgc gttcatagct
     1741 tttgccactt cgggtgtgat ttttgatttc gcatctagta atgttccatc aagatcaaag
     1801 aaagtgattg cacgataatt tgacacaaat aaaactcctt ttttattgga ataagtttat
     1861 cttaacaaag aatgaacaaa agacaaacct ttcgtgaaga attcctgaac atattggttc
     1921 agtcaacaat ttagagtata ctataagaag agaatttagg agggaaagaa tgattttttt
     1981 cagtattggt atcttattct taataattgg cagtcttttt cggattctgc cctcaaaagg
     2041 aaatctgcct ttttatgggt atcattcgcc tcttgccgca aaaacagacg ctcattggcg
     2101 gctggctcaa aagacaagcg gaaactggtt ttttcttatg ggcttactga tggcattgat
     2161 cggctattat ttaaagacat ctgggcatac gaattatttt ctgattgaaa tgcttctgct
     2221 agtatttcca attatgccta tttttattat gacagaaaag aaattacaga aatatgacct
     2281 ggaaactgga gggaacgata atgaatattt taatgattga agataatcaa tctgtttctg
     2341 aaatgatgca gatgtttttc ttaaatgaag gttgggaagc aactttcaag tatgatggaa
     2401 aagaagggct cgatgccttt ttagaaaacc cacaacattg ggatatgatt acactagacc
     2461 tgaatttgcc cacactggac ggtatgctag taggaagaga gatccgcaaa gtttcaagta
     2521 ctgtgccgat tatcatgctg acagctagag attctgaaag cgatcaggtg atcggcttag
     2581 agatgggggc agatgattat gtcacaaaac ctttcagtcc tctgacgctt attgctcgaa
     2641 tgaaagcttt gcatcgccgt agtgagttgg cagaagcaag agaagaagca gtagtttcag
     2701 atgatcattt tgatatcgag actagtcatt tcaagatgaa tacgaaaaca cgagaagcgt
     2761 atctggacaa ccggccaatt gaaggactga cgcctaaaga gttcgatcta ttgtttactt
     2821 tagcaaaaaa accacgtcaa gtattctctc gggagcagtt gcttgaatta gtctgggatt
     2881 atcagtactt tggtgatgaa cggacagtgg acgcccatat caagaaatta cgacaaaaaa
     2941 tcgaaaaggt cggaccacaa gtcatccaaa ctgtttgggg agtcggctac aaattcgatg
     3001 attctggtgt tgcctaatgc gttatttgta tcagcaactc ttagctttct gcggaatggt
     3061 cgcgttgatc ttattgatcg ttggagtttc ttttacacaa ctgacaaaac aaacgattga
     3121 agaaaacaat taccagcaac tgtttggata tgctgaatca gttgaaaaga caacgcaaac
     3181 ctatgccgat acgctcccgc agtttagtca agatcaggcg tttcaaaatg cattgctgct
     3241 gacagagcaa gtactgacag aacaaaatgt gaactttata tttatcgata aaaacgaacg
     3301 tgtcatttat ccgactaata cagctttact gaattttacc ttaacagctg atcaatggaa
     3361 aaatctgagg aatggacgtc aagaaaaatt cacatcgaat aaaaatattt taggacaagc
     3421 acaggcgacc tcttacgcac ttgtgccatt taatttgaat catgaattct atggagcact
     3481 tgtagttagt caacctgcgc gaaatatcga taacagcgtt cgtccaatta cactgaacct
     3541 gttcaaagga tttatttttt ccagtatcgt tgcagttatt gccagctatg cttttgcagc
     3601 attccaagtt aagcgtatca atcggttaag gaatgcgacg aaagaagtga cgaatggtaa
     3661 ttttgatgta cagctccccg tacatgacaa agatgaattt gatgagttag cagatgattt
     3721 caataaaatg acgaattcat tgaaagaatc tcaagcagaa attgaagaac aagaaaatcg
     3781 tcgaagacaa tttatggcag atgcttctca cgagatgaga acacctctta cgacaatcaa
     3841 cggacttctg gaagggttag aatacaatgc aatccctgaa aatcagcgag aaaacgcgat
     3901 taaattgatg aaaaatgaaa cagagcgtct gatccgtttg gtgaatgaaa acttggatta
     3961 tgaaaaaatc cgtacgaatc aaatcagtat ggtcattaaa aaattcaatg gaacagaaac
     4021 attgaaaaat attgttgcac agttagaagc taaagcagaa gcagcaggag atacattgac
     4081 attgaaagcc gttgatgata ttgatgttta cgctgattat gaccgatttg tccaaatcat
     4141 ggtcaatatt attcaaaacg cgatacagtt tactgaaaac ggccaaatca cgattacttt
     4201 agaaaaaggc tatcttgaaa cgatcatcac gattgaagat acgggaattg gaatgtctga
     4261 acaacaaatg aaaagtatct gggatcgtta ttataaagta gatccttcaa gaaaaaatac
     4321 aaaatatggt gaatcaggat taggtttacc aatcgtgcag caacttgtac gcttgcataa
     4381 aggaaaatta gaagtagaaa gcgaactagg aaaaggaaca aaattcgctg ttcgtttgcc
     4441 tgacgtagaa atcaaaaaag agtaaaaaac aagcggtata aaagatcatg aatcgatctt
     4501 ctataccgct tgtttttact ataaaatgat tgatttagcc cgcgggctct aaacgatcaa
     4561 tataaggact ttgagcaata gtagcatcat gagtaaagag taagatacct tgattttctt
     4621 catgagctaa ataagaaagc aagtggagta gctcttggat ctccaataca ctaagttcat
     4681 caaatgtatc ttcaataaca atattgtttc gcttggaaag caaggcatga agtaattgca
     4741 gctttacttt ttcaaaagac gtcaactcag ctgccgcaca attaagcaag gaaggagaga
     4801 tatcaagttt ttcttgatag gccaaacgat cttgtttgat ttctttttcc ggtacatcca
     4861 acaggacatg gtctttaaga ttcagataag gaaacatcgg ccaatttttt tggatgatcg
     4921 caaaattatc cagttttgca acaagaacct cttttttcaa gtgctccaat attttatgct
     4981 gtttttcgag aggacacaaa ataacggtca agtgttcagc atgaatcaaa tattcaggtt
     5041 tcattacgtc caggcctcct actttgcaaa tgatggataa aaaaagtgaa agggaataga
     5101 ctaagaatga gacagcatcc actgagccca ataagagttt gggcaaatcc cttaagtata
     5161 tctgtgaaaa aaagatcatt ttggaaatcg atggtaaaaa aagtctgttg atccatcgga
     5221 attgtaagtc cgcttaccga agtcaagata tcacggatat ctggcagttc agtagtcttt
     5281 aagacaagaa aattgatcct ttgaagaagc gcttcataaa ctgactggaa gcttacgatc
     5341 cacaataaaa ccaagataca acaaagaaag agaggaaaga acagctgcca gaaaataaaa
     5401 aggaataatt tggttttaga aagaccagtc aggtgccaat tgatcaattc ctgcttgttt
     5461 tgcctgactg aatagtaaaa gaaaaagata aaaaggaagg caaatagaaa aaataaactg
     5521 aatatgcctt ttatataaaa tcggctagct aattctaaag ccggaagaag ctctgtagat
     5581 gcagaagcgc caataaatga aagccgatct tggacttgat tggctaaagc cgattgcaaa
     5641 tggtaaagag tcaggcatgc tatcaataaa aaagcagaaa agcttgaaaa taaacaatag
     5701 agtattgtct gctttttgta ataaattaat cgagcagtag catagcggat tattttcata
     5761 cttttcacct ctataaaaag tatagaaaat gaatatgaat aaagtatgaa attcctttag
     5821 tgaaatcacg aattaaaacc agaaaatatt ttgtaaaaag caagaggttg tgaagaagct
     5881 gccctattct attaccgtag atgcaagctt ttgaacactg tttattcgaa aagaggagca
     5941 gcgacaataa ttttgtcgct gctcctttta aatttatttt atcattcgga agaacttgtg
     6001 gtcgttccgt gaagttcttg gtacgtttgc gttttataaa gatcaacagt tgattgtccg
     6061 ttgtgttcac taaagacact agttgacttg tcaccaagag atttttcagc ttcttttaat
     6121 ttctctaagc cattcttgta gtcataatca ctggtatcaa ttgggtctaa tccactattc
     6181 gtatagaacc gaagaagatc accattattg atttggtcag aaacagctaa ctgtttatca
     6241 actttttctt tcagtgaatc aatttcattc tgtacttcct cagtaggttc agtaatagct
     6301 tcaccagttt cgttactata gatcgtacca ccgtagtaag tatattttgg tgtaacaaaa
     6361 tcaccatcac ggaaagaaac aagctgttca tgatctttcg agaatagatc ttgtcctaac
     6421 tgaatataat tttttgtatc tacacctagt aagtgaagta atgtaggcaa ggcatcaact
     6481 tgtccgccgt aagtgtgatt gattccgccc tgtgtttgtc cagggatatg gatcatataa
     6541 gggacacgct gcatttgtgc attgtcgtag tttgtccaag tagaacttgt ttttccaact
     6601 aattcagcta gatttttatt tcttgaattc gagacaccgt aatggtcacc gtaaaggacg
     6661 atgactgaat tatcatacaa gccgctttct ttcaaataat tgaagaattc ttgtacggcg
     6721 gtatctaaat aattggctgt cgcaaagtaa ccgttgatcg tttcatcatt tgtattggct
     6781 aacgggaatc ctgcttcgtc attggtgaat tttgaataag gataatggtt agatacagca
     6841 atgaattttg aataaaatgg ttgttgcaga cgttccaagt attgtacgga ttgttggaaa
     6901 aatggtttat catgcaaccc gtattggaac gaattgtcat cattaacatc ataataagaa
     6961 gaatcaaaga aataatcata tcctaaattc ttgtatgtct cgtttcgatt ccagaatgta
     7021 ccagcgttcc catggaatac cgcactaatg taaccttgtg tttgacttaa aatatcaggt
     7081 gcagcttgga aagtattttt tccgcctaat tgtgtgaaca aggagccttg attcaagcca
     7141 aacaatgaat tttccatcag cgtttccgca tcgcttgttt ttcctgcttt tacttgatgg
     7201 aaaaagttgt caaaactgaa cgtgctgttg ctgtggtaaa gactattgat gaaaggtgta
     7261 acttcatgct ggagaccatt ctcatctgtc aatttataat cgatcaggaa ttgctggaag
     7321 ctttctaaat ggatataaat cacatttttt cctctagcga gcccgaacaa atcgtcattt
     7381 ggtgctgcat aatgttcttt tacataattt tctacttctt tcatatcatt gggacttgct
     7441 tctgcccgca cttggtttgt ctggtaagtt tgaacaccgt cataaaccat gaaagcattc
     7501 aagccaagat atttgaccaa ataatcacga gagaatgttc gagtcagcaa ttctggacga
     7561 tcagcttccg ccataaaaag atttcctgag aaaatcataa ctgctaatgt agagatcgca
     7621 aaagccattc ttgctcggat cgggcgaggg tccaacttga ttttttttgc aatcagcaga
     7681 acagtcaaga taataaaatc gatccaatag atcacatcat atgggcgaaa cagtcgaatg
     7741 gcactttctc ccagaccact cgctactttc ccagcaccta aaatcgtgtt gatcgtaatg
     7801 aagtccgtga attctcgata ataagtgaca ttcgaaaaaa caagcaatgt caaaagaaaa
     7861 taaatgacca tcatcgtgat ataagcaact ctagggcgtc tgatataaag agcaattcca
     7921 agtaataaca atgtagttgc aatagggttg ataaacaaga taaagtattg aatgccactt
     7981 tcgattccga gatggaaatc aactaaatag gcaaaaatcg ttttcaacca aagtaaaacg
     8041 actagtaaag aaaaaaatcc gagcctctta ttcaaaaagg caggtgtttt tatacatttc
     8101 aagataatct tcctttctgc acttcttaat cacagaagtg tcctgaaacc atgttacaca
     8161 gctcacttat tctcatttat tttactagta tgacaatata gtgtcaattg aaaccagaag
     8221 tttatgctgt ttcattaagg ttttctttaa gcttcatgaa aaaatgagca gtaagacaag
     8281 tcctatgttt tttgttatac ttgggaagag aaagctaaag caggtgaagg aatgaaagta
     8341 aaagtaaaaa aaagagcaag caatcgattg aaaaaaggat atccactgat ccaaaaagaa
     8401 gaccttgctc atccccaaaa agaaaccacg caatgggtgg aattagttga ttcgcaaaac
     8461 caatttatcg caaaaggata tctaggaaag cagaataagg gaatcggttg ggtattgagc
     8521 cagcagaaca tcccatttga tcaatctttt tttgaagaat tttttgtgaa gtcaaaagaa
     8581 aaaagaactt tctttttttc agatgattct actaccgctt ttcgtatatt caatggagaa
     8641 ggagacggga tcggcggtct gattgttgat tattatgcag gttatgctgt tttttcttgg
     8701 tataacgaaa cgttatatgc acatcgtgaa ttattgattc aagcttttcg tgaaatctac
     8761 ccggaaatca taggaggcta tgagaaaatt cgatttgaat cagtaaatct gcctgagagc
     8821 cagttcattt acggaaaaga ggcaccggaa cccttgatcg taaaagaaaa cggagtaaaa
     8881 tttgccgttt atttaaatga aggattaatg actgggatct ttttagacca aaaagaagta
     8941 cggggatcat tagcagaagg tttcgcttct gggaaaacag ttttgaacat gtttagttat
     9001 accggcgcct tttctgttgc tgccgcaatg ggaggggcaa tcgaaacaac aagtgtcgat
     9061 ctagcaaaac gcagtttaaa aaaaacacag gaacagtttg aagtgaatgg tttggatgtc
     9121 gatcagcaaa aaatcattgt tatggacgta ttcgagtatt tcaaatacgc caaaagaaaa
     9181 caatttttat atgacatgat tgtattggat ccaccaagtt ttgccagaaa taagaaaaaa
     9241 gtctttcgcg tagcaaaaga ttatggggaa ctggtcaagg atagtctgga tattttagca
     9301 gaagacggga tacttattgc gtctacaaat gctgcgaatg tctcgataga acaattccaa
     9361 acaatgattg aagaggaatt ccatgccaaa aatgtttctt ttgaacaact tgctttatat
     9421 cgtctgccaa aagatttcca gacgacggat acctttttag aaggcaatta tctgaaagtg
     9481 tttgtttatc aaattaaaaa taaccattag aaggagcgat gcagaatggt tcgggtaaag
     9541 ggattgaata taggaagcgg tagaccaaag atttgtgcgc ctcttatcgg ggccgtccga
     9601 gaggaagtat tacgtgaagc agttcttgca cgtgaagcag gggccgattt agtcgaatgg
     9661 cgagtcgatc attatcgtga agttttttct tttgatgaag tagcagaaac attggttctt
     9721 atccatgaga tgcttgatgg actgccgtta ttatttacgt ttcgtacctt agctgaaggc
     9781 ggagaacaag acatatcgat ccgtcagtat cgcgaccttt atgaacatgt gatacacaca
     9841 ggattagtag atatggtcga tatcgagttg ttcaaagtag aaagtctagg aaaaagactg
     9901 atagaggaaa tcaagtcttt aaacattcct ttaattatca gtagtcatga ttttaaagaa
     9961 acaccagcag accctgtctt gctttaccga ttgaatgtta tggaacattt tggagccgat
    10021 atcggaaaac tggcagtaac tcccaataat gaacgagatg ttttgcggtt gatggagttg
    10081 actagacggg caaatgcttt tgtctccatg cctattatta cgatgtccat gggaaatctt
    10141 ggtaaaattt ctcgtctggt tggcggaacg acgggttccg ttatgacatt tggttcctta
    10201 agcagcgatt ctacttctgc tccaggacaa ttaccggtca aagcattgaa gcaaattatt
    10261 caattattaa atgaaccgtt gtagaacaag tttgaggtga tattttttgg caaagaaaaa
    10321 aatagtaaaa acgaatgcaa tccgtatggt cgaacaaaaa aagattccct atacagagca
    10381 cgaatatgag tgggacgaaa gccacctaag tgcgtcatcg gtcgctgaac agcttccaga
    10441 aagccaatct cgtatattta aaacactggt tgctgtgggc aatgtgacgg ggcctcttgt
    10501 tgctgtgata ccgggagaag ccgaattgaa tctaaaaaaa ttagcaaaag tttcagggaa
    10561 taaaaaagta gaaatgctcc atttaaaaga tttggaagca acgaccggat acatacgtgg
    10621 tggctgctcg cctataggta tgaaaaaact atttcctact tatttagatc aaattgcaga
    10681 aagctatgaa caaatcattg tctcggctgg cagaagaggg ctacagatgg agttagcacc
    10741 tcaagatatc tgtgcattaa cgagcggaca atttgcagat ataaaacaat aaatcatacg
    10801 tataaaagaa agaaggaggc cgggatgttg tttgtccagt ctccttcttt cttttttata
    10861 cattatgctc tttgcccggc attcattcct gctacatgcc ctgtagcgaa agccgctgtg
    10921 atattaaagc cgccagtata accattgaca tccagtaatt ctcccgcaaa atacagacca
    10981 tcaatgattt tactctccaa tgttttagga taaatttcct ttagagaaac ccctccgcca
    11041 gttacaaatg atttctcaat aggaaaagtt ttgttgattg atagcttaaa ctctttgcat
    11101 agttgaacaa aatcttgaat ttgtttttga gaggcttgtt gtcctgtcag atgatccatt
    11161 tcaagccgct caagaaagaa aactaacaat cgctcaggca atataccatg ccaagcattg
    11221 accaagttct ttttggttgc tttacttttt tctgtcaata tatgaataag ctcttgtttg
    11281 gtttgtgtag ggaaacaatc cagagaaagt gtcactggtt ctcccgtttt ctctaattct
    11341 ttattgacaa aacttgaaca acgcagtgca gccggaccag aaataccgaa atgggtgaaa
    11401 agtaaatcca tcgtatgttc agtcaataca cgaccttttt tatttaaaac gcgtaaagtg
    11461 atgtcttgta aagaaagccc ttgaagtgtt ttctcctgaa tatacggttc atccgatatc
    11521 aagggggact ctgtcgcata aagaggcgtc accgtgtgac ccactcgctt cacgatcttg
    11581 tacccatctc cagtagaacc agtagatgga taagtccgtc caccagttgt taagataatg
    11641 gcaggagcat ggaactcttc gaattccgtg cgtataccgt aaattttatg ttctttgtgg
    11701 atcaatttct ctactcgagt agagaataga agcgtcacat ctagttcttt cagacgattg
    11761 agcaatgctt cgataatcgt ttttgattta tttgttacag gaaacatccg tccatgatcc
    11821 tcttctttta aatatacgcc ttgtgattca aagaagttca tgatatcgaa attattccat
    11881 tgcgcaaacg tactatataa aaactttcca tttccaggaa tatgtgcaat caaatcatca
    11941 acaggacgat tgttcgtaac attgcaccga ccaccacctg tcatcagtag tttttttcct
    12001 gcttttttgt ttttttcgat caagagtacg cgtgcgcctt gttcagctgc actgatcgct
    12061 gccatcatcc cgcttgttcc agcgccgact acgatcacat catacagttt ttccattccc
    12121 tcacctcaag gaaagtgtag catatctgaa gaaagaaaac agaaaaaaat gttttgagcc
    12181 aaaagagaaa atgagctaaa atgagggaag ataagcaaag gagcgtggaa cgatgacaaa
    12241 cgcaacagaa tatgttcaag caattcaaga aaagctacat caaaaggatc aagggcaaga
    12301 agaattttta caagcaatcg atgaattcat gcccacggtt atgtcttttt tggacactca
    12361 tcccgaatat atcgaaaaga atatcttaga acttttaacg gaaccagaaa gagttattca
    12421 atttcgagtg ccttggcaag atgatgaagg aaactggcga gtaaatagag ggtaccgtat
    12481 ccaatacaac tcagctatcg gcccttataa aggtggattg cgcttccatc ctagtgtcaa
    12541 tctaagtatc ttgaaatttt tggcttttga acagattttc aagaatagtt taaccggtct
    12601 gccaatcggt ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat ctgataatga
    12661 gatcatgcgt ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg gcccatcgat
    12721 tgatgttcct gcgggagata tcggtgtagg agctagagaa atcggctatt tgttcggcga
    12781 atataaacga ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt tggatttttg
    12841 gggaagtaag atacgtacgg aagctactgg ttatggatta gtgtattatg tcaaacatct
    12901 attgaacgaa gaaaaagatt catttaatca aaaaacagtt tttgtttccg gaagcgggaa
    12961 tgtagccatt tatgcaatcg aaaaagtgca agagctaggc gggaaagttg ttacatgttc
    13021 agattctagc gggtacatct atgatccaga aggaatagat gtgaaactat tgaaagaagt
    13081 caaagaaatc aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta
    13141 tcatgctgga gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc
    13201 tactcaaaat gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat
    13261 cgtagcagaa ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc
    13321 aggcgtgtgg tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt
    13381 agaaatgagt cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct
    13441 ggatcaaatc atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga
    13501 cgaaaaaaat ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat
    13561 ggcagcagaa ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt
    13621 ttatttgctt gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata
    13681 aggaggagaa caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa
    13741 cgaacaagag ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga
    13801 aggtactggc gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa
    13861 agaagaattt gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt
    13921 agttgttatc ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg acttcttgaa
    13981 tcacacattc ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc
    14041 cggaaattca atcagctcaa catatattgc tgacttgatc gaagtaatcg gtgatcgcga
    14101 cttttcagta aacgttattt caaaatctgg aacaactact gaaccagcga ttgcgttccg
    14161 tgtattcaaa gagcttcttg ttaagaaata tggacaagaa gaagcaaaca aacgtatcta
    14221 cgctacaaca gataaagcaa aaggtgcagt taaagtagaa gcagatgcag aaggttggga
    14281 aactttcgtg attcctgatg acatcggcgg acgtttctct gtattaactg ctgttggttt
    14341 gttaccaatc gctgttagtg gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc
    14401 aagcaaagca tactcaagcg acaaactaga agaaaacgaa gcttaccaat acgcagcaat
    14461 gcgcaacatt ctttaccgta aaggcaaagt aactgagttg ttgatcaact atgaaccagg
    14521 aatgcaatac ttctcagaat ggtggaaaca attgtatggt gaatctgaag gaaaagacca
    14581 aaaaggcatc tatccttcaa gcgcaaactt ctcaactgat ttgcactctt taggacaatt
    14641 catccaagaa ggacgtcgca atatttttga aacagttgtg aaggttgaaa aacctcgtaa
    14701 atcaatcaca attcctgaac aagcagaaga tttagacggt ctaggctact tgcaaggaaa
    14761 agaagtggac ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc atacagacgg
    14821 tgacgtgcct aacttgctag tcaaaattcc agaaatggat gcttatacat tgggttatac
    14881 aatgtacttc tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt
    14941 cgaccaacca ggtgtagaag cttacaagaa aaacatgttc gcacttctag gcaaaccagg
    15001 ctttgaagat cttgctaaag aattgaacga acgcctgtaa gataagggtt tttcgctaga
    15061 tttaaataat cagtgataga aacatgttcg ctttaaaatg aaaactggta cccatttagg
    15121 tactcaaaca tactgcatcg tttattcaga ataaggggct gtgacaagac gtttgtcaca
    15181 gtcccttgtt tgtatagaaa tcgattgatt gccttgctct ttagtttgtt ttaggacttt
    15241 aagaacagtg aggctgactt cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa
    15301 gcaaaaaagc actcacttcg agtgcttttt tgcgatatat tcttagctta agataggcct
    15361 aatttttctt ttacagatga gggtacctta tcgctggtaa ttactttttt ctctatgatc
    15421 cgcttttcgc ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga accattggca
    15481 gagtcaccag aagtaatgac atcgaaatct aactgtcttt tttcaccgtt ttcagcataa
    15541 gcatctacgt tgtatttaaa acctcttcca attacttccc cattatctgc tttgatatca
    15601 gtttctgcag gcattggagc tttgactacc gcataataat ctttacctac ataagtagac
    15661 tgatagtagt cccaaccttt ccatcctata aaagcaatca atatgataac gatcaaccct
    15721 aaaatttttt tcatgaaaga tctccttttt tatttttat
//
LOCUS       DAHHPA010000066        15536 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.067, whole genome shotgun sequence.
ACCESSION   DAHHPA010000066 DAHHPA010000000
VERSION     DAHHPA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.067"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(246..1811)
                     /gene="guaA"
                     /locus_tag="NBH55_001944"
     CDS             complement(246..1811)
                     /gene="guaA"
                     /locus_tag="NBH55_001944"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCD1509924.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1870..2793)
                     /locus_tag="NBH55_001945"
     CDS             complement(1870..2793)
                     /locus_tag="NBH55_001945"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCD1509925.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2989..3594
                     /locus_tag="NBH55_001946"
     CDS             2989..3594
                     /locus_tag="NBH55_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1509926.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3902..4918
                     /locus_tag="NBH55_001947"
     CDS             3902..4918
                     /locus_tag="NBH55_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCD1509927.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            5059..6360
                     /locus_tag="NBH55_001948"
     CDS             5059..6360
                     /locus_tag="NBH55_001948"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCD1509928.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6406..7068
                     /gene="deoC"
                     /locus_tag="NBH55_001949"
     CDS             6406..7068
                     /gene="deoC"
                     /locus_tag="NBH55_001949"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCD1509929.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            7089..7487
                     /locus_tag="NBH55_001950"
     CDS             7089..7487
                     /locus_tag="NBH55_001950"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCD1509930.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7553..8635
                     /locus_tag="NBH55_001951"
     CDS             7553..8635
                     /locus_tag="NBH55_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1509931.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8847..10409
                     /locus_tag="NBH55_001952"
     CDS             8847..10409
                     /locus_tag="NBH55_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509932.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10402..11535
                     /locus_tag="NBH55_001953"
     CDS             10402..11535
                     /locus_tag="NBH55_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509933.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11535..12491
                     /locus_tag="NBH55_001954"
     CDS             11535..12491
                     /locus_tag="NBH55_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509934.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12590..13756
                     /gene="deoB"
                     /locus_tag="NBH55_001955"
     CDS             12590..13756
                     /gene="deoB"
                     /locus_tag="NBH55_001955"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCD1509935.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13780..14598
                     /locus_tag="NBH55_001956"
     CDS             13780..14598
                     /locus_tag="NBH55_001956"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1509936.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14615..15322
                     /gene="deoD"
                     /locus_tag="NBH55_001957"
     CDS             14615..15322
                     /gene="deoD"
                     /locus_tag="NBH55_001957"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1509937.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         4868 a         2853 c         3478 g         4337 t
ORIGIN      
        1 atcttttaaa tcgaaaaatc tgatatatat atttatagta ctaaaaatct gattagataa
       61 aaagctaatt caataggaaa ataaaggtag tgaataaaag caaagccctt gttagtagtt
      121 aatctaacaa gggctttggt agggaaaatg gtaggaaacc atcttatttt ttacgaaaat
      181 caggaacttt agtaattgaa taatgttgat ttataggtat tcctcatcca tattatgttg
      241 gctctttatt cccactccac tgttgctggt ggtttagaag tgatgtcata cacgatacgg
      301 ttgacgtggg ctacttcgtt tacgatacgt acagagattt tttgcaagac ttcccatggg
      361 atacgggcaa agtcagcggt cattccgtcg attgaagtta cagcacggat accgactgtg
      421 tagtcgtaag tacgtccgtc acccataaca ccgacagagc ggatccctgg aagaacagtg
      481 aaatattgcc agatatcacg gtcaagtcca gcagctgcga tttcttcacg caagatagca
      541 tcagattcgc gtacgatttc tagcttgtct tcagtgattt cgccaagaac acgaatacca
      601 agaccaggac cagggaatgg ctgacgccaa acgattgaat caggcatgcc taactgagtc
      661 cctaattcac ggacttcgtc tttgaataat gtgtttaatg gttcgatcaa ttcaaattgc
      721 atatcttctg gcagtccacc tacattatgg tgagatttga tcgtctgtgc tgtttctgta
      781 ccagattcga taacgtcagt atacaacgtt ccttgtgcta ggaaagagac gccttcttca
      841 ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga attcattccc aatgatttta
      901 cgtttttgtt ctgggtcaga tacacctttc aacttgtcta agaagcgatc tcttgcattg
      961 actttgatga tgttcaagcc gaattttccg cctagacttt ccattacttg ttccgcttcg
     1021 cctttgcgta gcagaccgtg gtcaacaaag atagatgtca attggtcacc gatcgctttt
     1081 tgtaaaagaa ccccaacgac actagaatca acgccacctg aaagaccaag caagactttt
     1141 ttgtcaccaa cttgttcacg gattttttct acttgcatat cgataaagtt atccattgtc
     1201 cagttacctt cacagtgaca aacatcaaag gcaaagtggc gcagcaattc gattccgtat
     1261 tcggaatgac gtacttctgc atggaattgg ataccataaa aattacgtgt tgtgtcttgg
     1321 atagaagcaa tcggacaatc tttgcttgtt ccgactgttt caaagccttc aggaacttta
     1381 gttaccaagt caccatggct catccatact gtttgttgtt ttggtgtttc tttaaacaag
     1441 acagcttgat cgcttgttac ttctaacaag gctttcccat attcgcggtt tttagcaggc
     1501 tctacggaac cgccaagttt gtacgtgatc aattgcatac catagcaaat ccctaataca
     1561 ggaataccta aattgaagat ctcaggatcg atcccaaatg cattctcatc atatacactg
     1621 tttggtccgc cagaaagaat gattcccttt ggattcatcg cttttacttc ttctgcagta
     1681 atgcggtggc tcaataattc ggaaaagact ccaagatcac gaatacgtct tgtgatcaat
     1741 tggttatact gactaccgta gtctaatacg ataatctttt caacgttcgt catatcggca
     1801 acgtttgtca aatttattac ccctatccct ttaaatttta tctcaaaata gtcaaactat
     1861 tttaaaatgc taatattttc gcatgaaaat cgaatcgatg atatgatttt cagttttatg
     1921 gagaattata tcggctcttc ctcgtgtagg caagatatat tcctgtaaat ttttcaaatt
     1981 cacatttttc catacatctt ttgccatttt aaaggcttct tcacgatctc cgatagcaaa
     2041 acgataataa taattctctg gattttggaa agccgtatcc agtaacgcac caaaacgatc
     2101 caaataccat ttttcgatca gcacgggatc ggcatcgaca tagacagaaa aatcaaaaaa
     2161 gtcgctgacg tagatttgct gattcgcagg taattgaagc gtattgatcc cctcaacgat
     2221 caagatatca ggctgctgga tcaattcgta cttgtctgga atgatatcat acacatcatg
     2281 tgagtaaaca ggtactttta tctcttcctg accagatttt acacggttca agaaatcgat
     2341 cagcatctcc atatcataac tttcaggaaa tcctttacgg tccatgattc cacgctcttt
     2401 taatatacga tttggataaa gaaatccgtc agtcgtgatc agctgaacgt ttcttcgttt
     2461 gaaggtacga gacaaaatcg tctgcagcag tctggctgtt gtactttttc caacggctac
     2521 acttccggcg atcccgataa taaaaggcgg tacttctacg tatttatgaa gaaataaacc
     2581 tttactcaag aacaaagatt caaattcttt catataaaga taaatcagat gagcaagagg
     2641 tacatagata tctcgaacat cctgcatgga aatgcggtca ttcaaacttt tgatattatc
     2701 tagttcatct tgtgtcaaag gtgcttgccc attacgataa aattctcgcc attcttctct
     2761 tgccacacgg tagtagttca ttggatcatt catattagcc tcctgcgttc tcccatcaat
     2821 ggaactattt taacacaaaa taaacgcaaa ccccaccact taagagagat tgccgatctt
     2881 tttgtataat ctttataata gaaagaaacg gagtcttatt cttgacttgc gcacggatac
     2941 ataaaaggat agactgttgt catgaaagaa atgagaagga tggaagacat gacaaatcat
     3001 tactattctg aacgcccaga tacggcgcac gattttgaac aatgggcttt tgagttaaaa
     3061 ggcagaacct ttcaatttgt aacagattct ggtgtatttt cacgtgaaac agtggattat
     3121 ggttcacgtg tcctgattga tgctttcgat tgggaagagt tacctgatga aggaaagatc
     3181 ttagatgtgg gatgcgggta tggaccgatt gggttagctt tggcatttgc gacacagcgg
     3241 tttgttgaaa tggtcgacat taatgaacga gcggtaagtc tagcgcaagg aaacgcaaag
     3301 cgtaaccaaa tcgaacatgt tgatatccat caatcgaata tctatgaagc agttcatgaa
     3361 gaaacctatg cagctatcgt aagcaatccg ccgatccgtg ctggaaaaaa agttgtacat
     3421 gaaattttga caggcgcata tcctcggttg aagaagggcg gtacactgac aattgtgatc
     3481 cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg aagaaacatt tggtaatgca
     3541 gaaatcgtca caaaagataa aggatattat atcatcaaaa gtgtgaaaga ataactagct
     3601 gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc atgtacgggt acgtatcagt
     3661 tctttttttg ttgagaagtt tttaaaaaca aagaactatt ttttcttcaa aaagaaatag
     3721 taggaacaga gtcgttgttt tttacgataa aataaataag atacagaact tgtatagaag
     3781 gctgccaagt cggtagataa aggaggtttt tatcaaaaaa catactttcc ttttttctct
     3841 attcttttct tgggggtttt ggtacactga tagggaattt agatagaagg gagccaatag
     3901 gatgaagctg accatcagag atatcgcaga aatggcaggc gtatcagtaa caactgtctc
     3961 acagattttg aataacaaag gaagcagatt tagtgataag acaagaaaaa aagtgttgtc
     4021 gatcgtgaat gaacatcatt ataagcccga ttatttcgct tcaaatgtga tcaatcgaca
     4081 ttcaaagacc atcggaatga tcgtccctga tgtcactgat ttcttcttct ccaaactgat
     4141 cgaaggtgta gagagctatt tgaatccttt gggctatgtg atcatgttat gcaattctcg
     4201 tcatagccaa gaaaatgaga tcaagtattt acaagagctt tcccaccgct cagtggacgg
     4261 aatcttgctg gccacaccaa atattttacc tgagaaatat gcattagaca gcggttttta
     4321 tcaaaaaatg ccaatcattt taatcgatcg cggattgaat cgtcgggata acggacggct
     4381 tatcgtaaag gaatatgagg gcgcctatca agctgtttcg ctgtttattg aaaatggcca
     4441 tacgaagatc ggcatgctga aggaaaccac tggatactat caattagaag aacgtttcaa
     4501 cggatatcgt catgcgctga aagatcatgg cctgcctttt aatgggaaat ttgttgaaca
     4561 tggagagttg actgtccaag gtggatatac tgcatcaaaa aaattactca aacataaaga
     4621 tatcacggct attttttgcg ggaatgatgc catggcgatc ggatgctatc aggcgatcga
     4681 tgaattgggc aaaaaaatcc cagaagatat ctcagtcata ggattcgatg gattaaagct
     4741 gtcagaatac atgattccgc aattgacgac agtgcagcag ccaagttttg atattggatt
     4801 ttatgctgca cgtttcctga tcgatacgat cgaatttcct caaagaaaag ttccgaacaa
     4861 agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa agggctaact
     4921 cggcggtata ttgcttatgt aagcatttac aaaggctagt tagtgctttt tttattttta
     4981 gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat aatgtataat accgaatgaa
     5041 gacaaggagg atcaggcaat gcgaatggtg gatctgattg aaaaaaagag ggatggaaac
     5101 gagctgtcga aagaggaaat cgagtacatc gtcacgaact atacgaatgg aaagattcca
     5161 gattatcaag tcagtgcatt attgatggca attttttatc aagacatgac aaatgaagaa
     5221 atcacgaact taacattggc gattgctaat tctggtgatg tcatcgattt gagttctctt
     5281 gaaggaataa aggtagataa gcattcgaca ggtggcgttg gtgacacgac cacattgatt
     5341 ttagcccccc tagtagctag tgtcggagtg acagtggcta aaatgtctgg acgcggtcta
     5401 ggttacacag gtggtacact agacaagcta gaagcaattc ccggatttca gatcgagctt
     5461 tcggatgaag aatttgtacg cattgttaat gaaagtaaag tagcggtaat tggtcaatca
     5521 ggaaacttgg caccggcgga caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt
     5581 gattctcttc cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc aggagcagat
     5641 gcaatcgttt tggatgtgac cactggagac ggtgctttca tgaagaacat cgaagatgca
     5701 agacgtctgg caaagaccat gacgagcatt gggaaactgg ctaaccgaga aacagtggcg
     5761 gttatctcag atatgtcaga acctcttggc gaagctatcg gaaatagtct agaagttgtc
     5821 gaggcaatcg aaacattaca aggaaatggt ccagaagatc tagtagaaat gtgctacgcc
     5881 ttaggaagcc aaatggttgt tcttgctggc aaagctaaga caaccgatga agcaagaacg
     5941 ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa
     6001 aatcaagggg gagacccagc gatcgtggaa catccagaac gaattttgac tgcacgctac
     6061 acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta
     6121 ggaattgctg caatgatgct aggtgctggg cgtaaaacca aagaagaaga tattgatcac
     6181 gctgttggat tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta
     6241 ttgacgatct atagtaacga cgaagaaatc acttctgtca ttgaattatt atataaaaat
     6301 attgaaattg gtgaatcggc aatgaagcca acgttgattc atgacatcat cacagaataa
     6361 aggaaaaaag cggactttac cgttttaaag aaaaggggaa aagaaatgga actaaaccga
     6421 atgatcgacc atactatttt gaaagcagac gcatctaaag aagctgtgat gcaaattatc
     6481 gaagaagcaa aaaaatacca tttttattct gtctgcatca atccagcatg ggttgcgcta
     6541 gcgaaagaac aattacaagg tgagccagta gccgtatgta cagtaatcgg gtttcctcta
     6601 ggagcaaaca cacccgagac aaaagcattt gaaacaacag atgccatcaa taacggtgca
     6661 gatgaagtcg atatggtcat caatatcggt gctttgaaat caaaggatga tcaacaagtc
     6721 caaaaagata tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt
     6781 attgaaacat cgttattaga tagagaagaa atcatccgag cttgcgaaat agcaaaagct
     6841 gcaggcgctg attttgtcaa aacatcaact ggtttctcaa caggcggagc aaaggtagag
     6901 gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt
     6961 atccataacg aagaagaagc aatggcaatg atcgaagctg gtgctacaag aatcggaaca
     7021 agcgcaggag tagcaatcgt atctggttca actggtgaag gctattaatt ttttcaaagg
     7081 agaagctcat ggcagaactc aaacaagaat ggatcaacac agctattgaa gcgttagata
     7141 aagcctatgt tccttattcc cattttccgg taggcgcttg tctcgtaacg gaatcaggca
     7201 agatttatca aggcatcaac attgagaatg catcatttgg attaacgaat tgtgccgagc
     7261 gcacagcttt tttcaaagca gtatctgaag gagaaaaaac atttacgcat ctagtcgtag
     7321 cggggcacac ccccgatcct atctctcctt gtggtgcatg tcgacaagta atggcagaat
     7381 tctgttcccc tgatatgccg gttacgctag taggagacaa tggtgtgacg aaggcaacga
     7441 ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga tctataactc atgttcattc
     7501 agtcagctga ctgttgaaaa ataaaacaat tattttagga ggctttacag aaatgaaaaa
     7561 agcaaaatta tttggtttag gtgctgtcgc actagcagca gggttatttt taggtgcatg
     7621 cggcaacaac ggctctactg attcatccgg tggcaaaagc tcatcagaca caacaactgc
     7681 agcattgatc acagacacag gtggtgtaga cgaccgttca ttcaaccagt cagcttggga
     7741 aggactagaa aaatggggga aagatcaagg actttcacga ggaaatgatg gcttccaata
     7801 tttccaatct tcaaatgaat cagattacat cccaaatatt gaccaagcat tgaatgctgg
     7861 attcaaaacg atttttggta ttggttataa actaaaacca gcgatccaag aacaagcaac
     7921 aaacaataca ggtacgaact ttgttattat cgatgatgtc atcgatggat tagataacgt
     7981 tgtatctgca actttcaaag acaacgaagc ttcttatctt gcaggtgttg cagcagctta
     8041 tacgactgaa acaaacgtag tcggctttat cggcggtgta aaaggtgaag taatcgatcg
     8101 ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat
     8161 caaagtattg aatcaatatg ctggtgactt ctctgctcca gataaaggac gttcaattgc
     8221 tcaaggtatg tatgcacaaa acgcagatat catcttccat gcttcaggcg gtacaggtaa
     8281 cggtgtattc caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag tttgggttat
     8341 cggtgttgac cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa
     8401 cttcacattg acatctacac tgaaagcagt tggaacagta gtagaagatt tggcacaaaa
     8461 atcagcggac ggtaaattcc ctggcggcga acacactgtt tatggattga aagaagacgg
     8521 tgttggtcta acagaaggac aactttctga cgaagcgaaa aaagcagttg atgaagcaaa
     8581 agaaaaaatc atttcaggtg acgtaaaagt tccagaaact cctgaagaaa actaaaacca
     8641 tttctatagc cttgttagac acatgtttga caaggctatg gtttgattag aacaagaatt
     8701 gttcatttat ccaagctgcc ttgtgacagt ttggataagg gaacaactcc ctgaagtaat
     8761 gtttgtttca aaagaagaac gctcactaag cgtgaatctt agtcaatcgt tgccctagat
     8821 agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga
     8881 atattaccaa agaatttgga acattcaagg caaatgacaa catcaattta caagtaaagg
     8941 caggagaaat tcatgcgttg cttggagaaa acggtgctgg caaatctaca ttgatgaacg
     9001 tgctttccgg attattagag ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa
     9061 agatcacaag tccgaccaaa gccaaccaat tagggattgg gatggtccat cagcacttta
     9121 tgcttgttga tgcctttact gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg
     9181 cagggatgct tgaccataaa aaagcacgaa aagagatcca aaaagtttct gaacagtatg
     9241 gattgtcagt caacccagat gcttacgttc gtgatatttc agttggaatg gaacaacggg
     9301 tagaaatttt aaaaacactt taccgaggag cagatgtact gatttttgat gagccgacag
     9361 ctgtattgac ccctcaagaa attgatgaat taatcgtgat catgaaggaa ttagtcaaag
     9421 aaggcaagtc gatcattttg attacgcata agttagatga aatcaaagca gtagctgacc
     9481 gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct
     9541 cacaacaatt agccgatatg atggttggaa gaacggtttc attcaaaacg atgaaaaaag
     9601 aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg
     9661 gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta cttggtatcg
     9721 ctggaatcga tggaaacggg cagtcggagt tgatccaagc tttgactggt ttgcgaaagg
     9781 cagaaagcgg acatatcaag ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa
     9841 tcactgaaca tggtgtagga cacgtgccag aagaccgtca taaatacggg ttagtcctag
     9901 atatgacatt atctgaaaat atcgcactac aaacctatca tcaaaaacct tacagtaaaa
     9961 acggcatgct gaattattca gtaatgaatg aacatgccag agaattgatc gaagaatatg
    10021 atgttcgaac aacgaatgaa cttgttcccg caaaagcttt atcaggcgga aatcagcaaa
    10081 aagcaattat cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt gcaaatccaa
    10141 ctcgtggact ggatgtaggt gcgatcgaat ttattcataa acgtctgatc gaacaaaggg
    10201 acaaatacaa agcagtgtta ctgatcagtt tcgaattaga agaaatttta aatgtttcgg
    10261 atcgtattgc tgttatccat gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat
    10321 ctgaaaatga attaggtcta ttgatggcgg gctatacctt agaagaagca cgaaaagagt
    10381 tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa aattccgtaa tttattagtg
    10441 cctgtacttt cagttgtttt aggattgatc ttaggcgcga tattgatggc tgcttttggt
    10501 tttcagccca tccaaggtta ttcttctatg ctagatgcag cccttggttc gcaaagaggg
    10561 ataggtgaaa cgttaaggca agcaacgccg ttgattttta cagcattagg tttttctgta
    10621 gctaactcag ctgggttttt caatattggg ctttctggac aagcactttg cgggtggatc
    10681 gtcagtgtct gggtggcact tgcgtttcca gatctgccaa aagttgtttt gcttccgtta
    10741 tgcgtgatct taggtgcctt agctggtgcg atggcagcgg ctgttccagg tttacttcga
    10801 gcattttttg gaacaagtga agtgatcgta acaatcatga tgaactatat cttattatac
    10861 gtaagcacat ttgctttaca tgaagtgatg cctgaaagct atcgctcaag tttggactct
    10921 tccaatatga tcagtcaaaa tgcctcactg cggttaccat ggttaacaca agcttttggc
    10981 ggctctcgcg taaactcagg attattttta gcaatcatcg cgcttatttt cgtttggctg
    11041 ctgatgaaga aaacgacttt aggatttgaa atccgttctg ttggattgaa tccttttgct
    11101 tctgagtatg caggaatgag tagtaaacgt acgatcgttt tgtcgatggt catttctggc
    11161 gctttggcag ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt
    11221 caaacgactt cgctatctat tggatttgac ggaatggctg tttccttgct tggttctgga
    11281 tcagcaatcg gtattctttt atcagcgatc ttgttcagta ttttgaagat cggtggactt
    11341 ggcatgcaga caggagcagg tgttccgttt gagatcgtca atgtatcgat tgctttgatt
    11401 atcttctttg tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt cttccaaggg
    11461 aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac caacgaatca aaaccatgaa
    11521 gggggcgaac tataatgtcg actatcgaat taattgcctt gatccttact tctacactgg
    11581 tttattcaac accgctaatt ctaacgtcac taggcggaac tttttctgaa cgaagcggga
    11641 tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc ttttagttcc atcgttttca
    11701 acttgacgat gagtccatca cttggttcta tgacaccatg gatcgctatt ttagtgggtg
    11761 gcgtcgtagg tatcctattt tctctgattc acgcagtagc aacagtcagt ttgcgtgctg
    11821 accatattat cagcgggaca gtaattaatc tgatggcacc tgctttaggt gttttcttga
    11881 tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac gcagaatctg gggtattttt
    11941 ctttcccagg attagcagat attccggtca ttggaccaat tttctttaaa aatacattct
    12001 tacctgctta ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc ttcaagacga
    12061 aattcggctt gcgcctccgt tcagttggtg aaaatcctca agctgccgac acattaggta
    12121 tcaatgtata tgctatgcgt tattcaggcg tactgatttc tggcttgtta ggcggtatgg
    12181 gcggtgctgt ttttgctcag tcgatttccg gtaacttttc agctggtacg atcgttggac
    12241 aagggttcat ctccatggct gctatgatct ttggtaagtg gaatccttta ggggcaatgg
    12301 gtgcctcatt gttctttggt tttgctcaaa gcttgagtat cattggcgca caattaccag
    12361 ttatttcttc gattccgcca gtattgctgc aaattgctcc atatttgctg acaatcattg
    12421 tgctggttgt tttcttaggg aaagcttctg gtccaaaagc aaacgggaag aactatatca
    12481 aatcaaaata atcttttagc gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg
    12541 ccctttttgt tttaaaggca ataaaaataa aaagaaagaa ggaataatca tgtttaaacg
    12601 cgtacatctg atcgtcatgg attcagtagg aattggagaa gcaccagatg ccgataagtt
    12661 cggtgatgtt ggttctgata cattaggaca cattgcgaaa gaagcaggat tgaacgttcc
    12721 acatttagag cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa
    12781 agatcatcaa ggctatgcga cgaaattaga agaagtttct gtagggaaag atacgatgac
    12841 aggccactgg gaaatcatgg gattgaatat caaaactcca tttcgtgtgt ttcctaatgg
    12901 attccctgat gatctgatca aacaaatcga agaattttct ggccgtaaag tcgtatgtaa
    12961 caaaccttat agcggaacag ctgttattga cgattacggt gaacaccaaa tgaaaacagg
    13021 cgatttaatt gtatatactt ctgccgatcc tgtccttcag atcgcggcac atgaagaaat
    13081 cattccttta gaagaattat atcggatttg ccaattcgtt cgtgatataa caaaagatga
    13141 tccgtatatg attggccgaa tcattgcacg tccgtacgtg ggtgaaccag gagacttcac
    13201 acgaacaagc aatcgccatg attatgcgtt gaatccattc ggacacacag tccttgattc
    13261 tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag atcaacgata ttttcaacgg
    13321 acaaggaatc actgaatctg tccgcacgaa aagcaatatg gatggcgttg accagctgct
    13381 gaaagttatg aaaaacgatt tttcaggaat cagctttacc aatttagtag attttgatgc
    13441 attatacggt catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag aatttgactt
    13501 acgaattcca gaattgcttg aagcgatgga agaagacgac ttgctaatga ttacagccga
    13561 tcacggaaac gatccgacgt ttactggtac cgatcatacg cgagaatatg taccgttatt
    13621 ggtttacagt aaaaaaatga aagaacaagg ggcattgcct caaggatatt attcagatat
    13681 ttcagcaacg attgctgaga actttagtgt ttcttcgact gaaaatggcg aaagcttctt
    13741 gaaattatta aaataatctt tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg
    13801 ttacaagaaa cgacccagtt tatccttgat aaaggagtaa aagaaattga ttttggtttg
    13861 attctaggtt caggattagg tgaattagca gatgaaatcg aagatgctgt aatgatttct
    13921 tatgatgaga tcccgttctt tcctgtttcg acagtagttg ggcatgcagg aaagctagtt
    13981 tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag ggagattcca ctactatgaa
    14041 ggtcattcca tgcaaacagt gacctatcca gtacgtgtga tggccggact aggggcccat
    14101 tcagtcattg tgactaatgc atgtggtggt gtcaacgaat catttgtacc aggagatttg
    14161 atgctgatta ccgatcatat caattttact ggtgaaaatc ctttgatcgg tccgaatgaa
    14221 gatgcaatgg gccctagatt tccagatatg agtcaagctt atacgcctga ctatcaagca
    14281 acagcaaaag cagcagcaga aaaactaggt ttggatctta aagaaggcgt atatatgggc
    14341 tattctggac caacttatga aacgcctgcg gagatccgaa tgtctcggac aataggagcc
    14401 gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag cagctcacag cggattaaaa
    14461 gtgttaggaa tctcttgtat taccaactta gcagcaggta tgcaagcgaa tctgagccat
    14521 gaagaagttg tagaaacaac tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa
    14581 acattgacat tattgtaata ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga
    14641 aggcgagatc gccgataaga ttttacttcc gggagatcct ttgcgagcta aatatattgc
    14701 ggagactttt ttggaagatc cagtttgtta caatcaggta cgcggtatgt taggctttac
    14761 cggcaaatat aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc
    14821 caccatctat gcccatgaat tgatccagtc gtatggtgta aagaaattga ttcgagtagg
    14881 tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat ttagtactag cacaaggtgc
    14941 agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag
    15001 tgatttcaat ctattactga aagcatatga gatagctaaa gaaaagggct atacggttca
    15061 tgtaggaaat gttctttcgg aagattcttt ctacaaagat gatttgacag agacgttcca
    15121 attagctgaa cttggcgttt taggagtgga aatggaagca gcggctcttt attacttagg
    15181 cgcgaaatac catgtgcaga cattaagttt gatgaccgtt agcgaccatt tgatcaccgg
    15241 tgaagaaaca acagctgcag aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt
    15301 ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct gccctgcatt aagtaataag
    15361 aacatccaag caaaaatagc tcctcatgtt tttgacttgg ctggacttat tattgcaaat
    15421 gtaagctttt gaacaccgtt taccaagagg ttgtgaatct gccgtttaac tctgagtcac
    15481 aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact tgtgac
//
LOCUS       DAHHPA010000067        15315 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.068, whole genome shotgun sequence.
ACCESSION   DAHHPA010000067 DAHHPA010000000
VERSION     DAHHPA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.068"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            85..3312
                     /gene="fss3"
                     /locus_tag="NBH55_001958"
     CDS             85..3312
                     /gene="fss3"
                     /locus_tag="NBH55_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCD1509938.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3425..3739
                     /locus_tag="NBH55_001959"
     CDS             3425..3739
                     /locus_tag="NBH55_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCD1509939.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3752..4126
                     /locus_tag="NBH55_001960"
     CDS             3752..4126
                     /locus_tag="NBH55_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCD1509940.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4136..4471
                     /locus_tag="NBH55_001961"
     CDS             4136..4471
                     /locus_tag="NBH55_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCD1509941.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4554..5894
                     /locus_tag="NBH55_001962"
     CDS             4554..5894
                     /locus_tag="NBH55_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCD1509942.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5972..6646
                     /locus_tag="NBH55_001963"
     CDS             5972..6646
                     /locus_tag="NBH55_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCD1509943.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6639..6803
                     /locus_tag="NBH55_001964"
     CDS             6639..6803
                     /locus_tag="NBH55_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509944.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6879..8021
                     /gene="dcm"
                     /locus_tag="NBH55_001965"
                     /pseudo
     CDS             6879..8021
                     /gene="dcm"
                     /locus_tag="NBH55_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8011..8301
                     /locus_tag="NBH55_001966"
     CDS             8011..8301
                     /locus_tag="NBH55_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509945.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8560..9744
                     /locus_tag="NBH55_001967"
     CDS             8560..9744
                     /locus_tag="NBH55_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCD1509946.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9741..9878
                     /locus_tag="NBH55_001968"
     CDS             9741..9878
                     /locus_tag="NBH55_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCD1509947.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            10624..12534
                     /gene="ltrA"
                     /locus_tag="NBH55_001969"
     CDS             10624..12534
                     /gene="ltrA"
                     /locus_tag="NBH55_001969"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCD1509948.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            12638..12862
                     /locus_tag="NBH55_001970"
     CDS             12638..12862
                     /locus_tag="NBH55_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509949.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            12875..13378
                     /locus_tag="NBH55_001971"
     CDS             12875..13378
                     /locus_tag="NBH55_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCD1509950.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            13438..13827
                     /locus_tag="NBH55_001972"
     CDS             13438..13827
                     /locus_tag="NBH55_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1509951.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            13814..>15315
                     /locus_tag="NBH55_001973"
     CDS             13814..>15315
                     /locus_tag="NBH55_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1509952.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT         5145 a         2539 c         3415 g         4216 t
ORIGIN      
        1 aaaaatttta aaaagacttt ataaacaaag atgttcaagc gagcatctta tttttttgtg
       61 cttaatttga aaggagaaca tttcatgtat aaaaaacaaa aaaagaaatt atgtgtatta
      121 actattttga gtttggctgt tcaagcaatt gttggagcaa ttgctcctac gattactttt
      181 gctgatgaaa ttacccatcc acaaacggta accgtagaac tagatttagc acatcaatat
      241 gctgttgaag gaacttttag tgatggtcgt ccaatgtcag aagtcactgt tccgcattat
      301 gcagtgtaca acggctttaa acaagatatt ttctgtattg aaccaggagt cccgatttac
      361 aatgagttca ctcctggcta tgagaaaaac ccgttaccag atatgtccga taaagccaaa
      421 ttagtttccg ttttatggaa aaaagtaggt acagatgcag atactcatat tgtggcacag
      481 aagatgatct ggcaagaagt taatgggtat acccttcact caataaaacg atcagatggt
      541 agtgccgtaa atatcgcagc cattgaggca aaaataaatc aagccattga cgattatcag
      601 aagaaaccaa ccttccataa tagtacggct aaaacagtat tagggcaatc gactactgtg
      661 acggatacga ataatctgaa tttgtcagag ttcgatgagg ttgtggaaaa tgccgcaaac
      721 attgattatc gtgtgaatgg taatcagttg ataatcaccc caaatgccaa ttcaaaagaa
      781 agtggtgtgc tgacgcttaa gaaatctgct ggtaccggaa cacctgtcgc ctataaaaag
      841 gctggccaac agactttaat ggccggggcg attgataaac ctaataccta cacaatcaaa
      901 attgatgtgg aaacggaggg ttctttaaag atcaaaaaag tcgataaaga atcgggtgat
      961 gttgtaccag gaacggtttt ccatttagac tttggaaaaa ctttacctgc aaaagacgtg
     1021 actactgaca aagaaggcat tgctacattg gatgagattc ctcatggtac aaaagtaacc
     1081 attaccgaaa aatcagtacc ggcgccttat acgattgata ccacccccat gactaccacc
     1141 attaaagcag gcgaaaccat ttacgtgact tcaaaaaacg cacgggaaaa agggcaaatc
     1201 attctggata aaagcggagt tgaaacaggg agtgatcttt ggaatgacaa ttattcctta
     1261 gctggtaata cgtttgccat tcgcaaagac agtccaaccg gtgaaatcgt tcaagaaatg
     1321 accacggatg aaaacggtca tgcggaaaca ccaaaagaga ttgctaatgc gttggaattg
     1381 ggaacctatt atgtgaccga aactaaagct agtcatgggt tcgtgaatac gttcaaacct
     1441 gtaaaagtcg aattgaagta tgccaatcaa accgtagccc ctgttaccag taacgtaaaa
     1501 gggcaaaatc aagaagtcac tggcgaaacc actttgacga aagaagacaa agatactggg
     1561 gataaagcac aaggtaaggc agtctttgag ggtgctgaat acaccttgtt tactgctaag
     1621 gatggaaaag ctgttaaatg gagtgaggct tttaaaccag agttagcgaa gggaacgaaa
     1681 ggttctgatg aaacggtcac tttgacttta gacgaaaaga accaagttgc cgttaaacat
     1741 ttagctatca acgagtatta ttggcaagaa acaaaagcgc ccgaaggata tagccttgat
     1801 gaaacaaagt atcctgtatc catcaaaaag gtggatgata gcgaaaaaaa tgccgtgatt
     1861 acccgaaatg ttacggcaaa agaacaagtg attcgctttg gctttgattt ctttaaattt
     1921 gctggatcag ctgcgggcac taccgaaact ggcttcaatg atctgacctt taaagtgtcg
     1981 ccattggaag ggaccaatga aatcacaggt gccgaagatg aagcgaccac agcttataac
     2041 gagcaattag ggtttgatgg ttatggcaag tttgaaaatc ttccttatgg ggattattta
     2101 cttgaagaag tagaagcccc agaaggtttt caaaagatta cgccattaga aatccgttct
     2161 acttttaagg aaaacaaaga agactttgtg aagagtgagt atgtctttac cattaccgaa
     2221 caagaccaaa aacaaccaat taaaacggtg accgttcctt acgagaagct gacgaacaaa
     2281 gcattttctg ttagtttgaa ccgtttaatg ctctatgatt tgcctgagga agaagatagt
     2341 ttaacttctc ttgcgacttg gaaagacgga aacaaagaat taacttctct ggattctacc
     2401 gagctagttg ataaattgag ttacaatcta catgaaatca aagaagactg gtatgtggta
     2461 gcgcaagcca ttgatgtgga tgcgacaaaa gctgcccaag aaaaagatga aaaagccaaa
     2521 ccagtggtga ttgcagaaac aagcgcaacg ttggcgaaca aagagaaaac aggaacttgg
     2581 aaaattcagc ataaattaac cgccgaacaa gttttaaata aaactattgt cttattcaac
     2641 tatgtgtatg aaaataagga agcttttgaa gcaggcgata agccagtagc gaaggatgtt
     2701 agcttgaata accaagccca aactgtcagt tgtacagtgg aacaccatgt ttctatccaa
     2761 acaaaggccc acctagaaaa cggttctcag acctttactc atggtgatgt ggtggatatg
     2821 tttgatgatg tgtcgattac tcatgatgta ctggatggct caaaagaagc tttcgaaaca
     2881 attctgtatg ctttattacc ggatggtaca aacaaagaaa tttggaaatc tggcaagatt
     2941 gattatgaag taaatgataa agaattcacc aaaactgtgc ttgcgaaaaa agtagatacc
     3001 ggaaaatatc cagaaggaac caagtttact tttgcggaaa tcaattacga taaagatggg
     3061 aatatcaatg gaaaacacaa tgaagatttg aaagaaaaat ctcaaacctt aacaccaaaa
     3121 gaagttccaa ccatactaag tacgccgaaa caaccggaaa caccaactgt tccaagtggc
     3181 tctcaagaat ctagtcccac agtgaagacc ttccctcaaa ccggggagaa aaattccaat
     3241 gttttactgt tcattggttt tactttgatt tttgcgacgg cgggctatta tttctggaat
     3301 cgccggaact aaggtgatga gatgatacaa gtaaatccta agaaccaacg tgcgcgaccg
     3361 cctcctaaaa aagtagtcgc gctatttttg tatccaaaac taacaatgaa aagaggaaaa
     3421 gaagatggaa ttgaaatttg tcgtgccaga tatggctgaa acctttggaa agttaagtta
     3481 tgccggtgaa ggagaaatta taacagaagg atatggacgg aatactacgg tgattggtcg
     3541 cagttatcat ttgtattcca gtaagcaacg ggccgatgat attgaggtgg tagtagctgc
     3601 ggaagccggt gaaaaggatt ttgatcaaga ccaaccgcta aaagccgtga atccccattt
     3661 ggttgccaaa ggctatgaga ttgagaatcg tgggtttact gattacgtgt tgtatgttga
     3721 tgatttagtg aaagcatagg aggaaaagaa gatgagatta acagaaggta ttgtcgtaga
     3781 ttcaggttta acatttggga agttgcgttt ttccgcatta cgccgagaag tacggaagca
     3841 aaatgaggat ggaacaattg gtaatgaggt aaaagaacga acctataatt taaaatcctc
     3901 tgcacaaggg cgaatgattc aagttagtat cccagctaat gtacccttac gcgagtttgc
     3961 ttatgatgca gaggtagagt tggtcaatcc aatcgtggat accgttgcca attatgtttt
     4021 tcgagaagga actaccgtta attggttcat taaggcggat gatttagtct tgaagcggca
     4081 gccgaatcaa ggaaatccta cgaaccaaaa tgaaagaaag aaataggtga agattatgga
     4141 aattaatgtt gaacatattt tagattgtct cgatcaatat ggaaaaggcg agctgactga
     4201 ggaacaactc gttaaagcat taacctctga tgaaaaaatg tttctgatta tacatcaagg
     4261 tttacttgga gaaaatagcg acagtgaaga agactctgat gtactagact ggttaggaga
     4321 agaagagtct ttctttatag ttgtcgaagt aaatgaaaac ttatgtcggc aggcagaatc
     4381 cattttggaa gaaattggtg tggagatgcc ggatgctatt gaaggctttt taaatcaatt
     4441 ggttgaaacg aaacaactcc cctcctcgta gcagtaaatg actagcataa ctttttgaaa
     4501 ttgcctaatg aaatcctatt gggctttttc gattggaggt ggaaaccatc aaaatgtaca
     4561 aaggacatcg catacgcgca ggagatcagc acttggttta tcactttgtt cttggctggc
     4621 tactcgctct gttcatcggt tggatgagcg tcttttattt tcaagagttt agacaatttg
     4681 atatttctaa gctatcactt tctactattg aaatcgtttg gtctataaaa gatttagtat
     4741 gtctgcttgg aagtctcgct ttttcaggag ctatgatatt gctttatatt cacttttttc
     4801 ttgatcattg gcgaagcttg tggcatcgac aaaagctagc tcggatgatt ctagagaacc
     4861 attggtatga agtgaaacaa actcaaagtg aaggcttttt caaagatttg aatagtagcc
     4921 gaaccagaga gactatcagt tacttcccca aaatctatta tcgaatgaaa gatggattac
     4981 tgtctattcg cgttcaaatt tcacttggga aataccaaga tcagctttta aaattggaaa
     5041 agaagttaga aagcggactg tattgtgagt tagtagaaaa ggaactcaaa gactcctatg
     5101 tggagtatac tttactgtat gatatgatcg ccaatcgaat tgggatagac gaagtagtgg
     5161 cagaaaatgg tactctgcga ttgatgaaaa atcaagtatg ggcttatgat tctttacccc
     5221 atatgttaat cgctggtggc acgggtggag ggaagaccta tttcctcctc accattatcg
     5281 aagcactatt gaagtcagat gcagaattgt ttatcctcga tcccaaaaat gcagatttag
     5341 ctgatttagg tacggtgatg cctcatgttt attcacaaaa ggaagaaatt tctgcttgtg
     5401 tggaagattt ctatgaacgt atgatggctc gtagcaaggc aatgaaagaa atgtctaact
     5461 acaaaacagg agagaattat gcctatcttg gacttccacc caacttttta atctttgatg
     5521 aatacgtggc ttatatggag atgttaacga cgaaagaaag tgcggtgatt ttgaataagc
     5581 tcaaacaaat cgtgatgttg ggtcgtcaat ctggtttctt tctgattttg gcttgccaaa
     5641 ggccggatgc gaaatattta ggagacggaa tccgcgatca attcaacttt cgcgtggctt
     5701 tgggtcgtat gagtgaactc ggatattcca tgatgtttgg ggaggtcgaa aaaaacttct
     5761 ttatgaagca catcaaaggt cgaggttatg tggatacagg tggcagtgtg attagtgagt
     5821 tttatacgcc acttgtacca aaagggtatg atttcttaga ttcaattagt aaattagaaa
     5881 agatgaatga ttgagtgatc ttcaatactg actttatact tcaagtaacg tataatactt
     5941 ttatatagta ggaagaaagg ggataattaa tatggaaagt aatgattctt atttagagta
     6001 tgcaaaagca aataaggata agtttatttc ttatattatc aaagggaaat cccccgaagt
     6061 tggacgagac gcagttttta tggcagggag tcctggggct ggaaaatcag aggttgccat
     6121 tggattagct gagaattatg ataaccatgt gattattgat gcggattact ttcgcaccca
     6181 gtttcctgat tataatggaa aaaactctag tctctttcaa aaagcatctt cttggttggt
     6241 agagcaggca tttagatatg tcttagatca tggctattct tttattctgg atgcaacttt
     6301 tgcaatatta agtgctgaaa aaaatatctc gcgggcgttg aaaaaacatt ttcgagtgac
     6361 gattttctat gtttatcaag accctaaaat cgcttggaaa tttacacaag aacgtgaaat
     6421 tgcagaggga cgtcatgtgc cgaaaaaggc atttattaac gcttttttca aatcaagaga
     6481 gaacattgca aaagtaaaga gtaaacatcc agatgtattg cttcatatca taataaaaga
     6541 ttatacgaat aatatttccg aagttcatta tgctacggat aatctagaat tatttttacc
     6601 actagggtat agtaggaaag atttggaggt tcaattacat gactgagaaa gaacaagtaa
     6661 cgaaaattgt aaagaaatac aataagagca ttgcggattt atcggaaaat gctactgcaa
     6721 aagagttcaa aacggtgatt aaatatgtag ccgatcaggc caatgaaaaa caacggaaat
     6781 tagttggatt aaataaaaaa tagtaggaca aattaataat gtatgagcca agagcaaaat
     6841 gttcttggct tttttctttg caatgaatga ggtaatgtat gagattttta gatttattcg
     6901 ccggtattgg tggctttcgt ttaggtatgg aacaggcgag ccatcattgt atcggttttt
     6961 gtgaaattga taaatttgct agacgtagtt ataaagcaat tcatgataca agtaaggagg
     7021 tggaaatgca tgacatcaca agtgtatcag atgagtttat tcaatccctc ggaccagtgg
     7081 acatcctttg cggcggattt ccgtgccaag ctttttcaat tgcgggaaag cggcaaagat
     7141 tttccgatac tcgaggtact ttattctttg aaatcgctcg gttcaccact cttctccgac
     7201 ctaagctttt attccttgag aacgtccgag gattgctcaa tcacgaagga ggggctacgt
     7261 tcgagacgat cctccgaacg ctggatggat tggggtatga cgtggagtgg taggtgctta
     7321 actcaaaggc ctacattccc caaagccggg agcggatatt ccttatcgga cattctcgag
     7381 acgcatgtac cgaacaagta tttcctatca ttggaccgtc ttcaacatct gatcaaaaca
     7441 tcagaaattt ggtaaatatc aatccttcca atcgaggaat gggtggacaa gtctatggtt
     7501 cagatggcgt agcgccaacc ttaacaggtg atgaaggaat caaaatcgca ctgccagcaa
     7561 gggatggaat ctctgtagca ggactattac cgggaaagtt tgaacaaggt aatcgagttt
     7621 atgaagttac tggaaccgcc cccaccttgt caacgaaaca aggtggtaca aaaatcatga
     7681 ttcgaaaaaa tgaaacagaa tatcaagagg tgaagccaga agacagtgtg aatctggctt
     7741 ttcctaattc cacaagtcgc cgaggtcgtc tgggaaaaca gagtgttcat accttactaa
     7801 caggagacca acaagcgatc gtcacggatc agtatcaaat tcggaagcta acacctagag
     7861 agtgttggcg acttcagggc tttcccgatt gggcttttga tcgagattct caagtcaatt
     7921 cagatagtca gttgtataag caagccggaa actctgtaac agttccggtc atttttgata
     7981 ttgccagaag attaaaagag ttggatgtac atggtttatg atgagttgat tggagaaatt
     8041 tattgggtga ttggaaagat tcagtcagac ccagaattag aagaacaatt gcgacggtta
     8101 aaatttgata ttcgcaagaa tggtgtcaaa gtcgctggcg atccatattt aatgaatgag
     8161 gagacggatg ctcgaattga aataaatcaa gtaattgcag aatttgagcg gattgcggac
     8221 caagcaaaag aaccagatat tcgtcaatac ttgttagaaa tgaaagcaga gttggaagta
     8281 aatggtatta cagatgagta acctaaacca acacaactga ctgttaggcg ctgaccgcgg
     8341 aggattcgcg aagcagaaat cctccgtggt gctgtgcctg ttcatggacg gcgttagccg
     8401 accatgatgt ctcacccccg tatctaacag gggggtacaa aaacacaaaa aataagtaag
     8461 caaccattaa gaatgcttgt tgtatcaaag atagtaagta atttgtatag gtgttaactc
     8521 atttgaaaaa gttaacacct tgaatttgga ggtgagaact tggcacaacg gaatttagat
     8581 tatcggctat taaaagaccg acgcaacgaa tacggcgttt cacaaaataa attggctacg
     8641 gcttgtggac ttagtcggcc gtacttaaat cagatagaaa atggtggcgt aaccgcatct
     8701 ataaaaacca tgaggaaaat ttttgaccaa ctggaaagct ttaatcccga tttgcctttg
     8761 acgttactgt ttgactatgt aaggattcgc tttcccacaa cggatgcacg aaaaattatt
     8821 caagaaattc tccatttgaa atttgagtat atgctccatg aagattatgc cttttactcc
     8881 tatcaagaac agtatgtcat gggtgatatt gtggtgatgt tgtctcatga agaagataaa
     8941 ggtgtgcttt tagaattaaa ggggcgaggt tgccgacaat ttgaaacttt tttactcgct
     9001 caaaagcgta gctggtacga ctttttcgag gattgtctaa aggccggtgg cgtgatgaaa
     9061 cgcttggact tggcaatcaa tgatcgagtg gggctattgg atatacctga gctaacgaaa
     9121 aaatgtcaga aggaagaatg tatctccttg tttcgtacct ttaaaagtta tcgttccggg
     9181 gaacttttaa aggctgatga aaaggacggc atgggcaata ccttatatat cggcagtctc
     9241 aagagtgagg tttatttctg tttgtatgaa aaagactatg aacaatatat caagttaggg
     9301 attccattag accaaactga gacgaaaaat cgttttgaga ttcgactgaa aaatgaccgt
     9361 gcttttcatg cgattcaaga tttgttaaaa ggtcgtagca ttgagagcac cacgttttcc
     9421 attatcaatc gttacttgcg atttgcggat aaagtagaag gtaaaaggcg aaccaactgg
     9481 cccttgaatg aacaatgggg gcgttttatt ggacggaatc ggaaagagat tcaattaact
     9541 tccgagccaa aaccgtatac cattgaacga accttgaatt ggctaggccg acaagtcgcc
     9601 cctacgtgga aaatggctaa ggagttggat cgattgaatc ggacaaccta tatccaggat
     9661 atggtacaga atgcacgctt atcagaccga cataaaaaga ttttggagca acaaagtatg
     9721 gcaattgaaa atttgattat atgaaaagag gaatcgtcat gggaattgta aaagatattt
     9781 ttttggtatt tggtggcagt atgcttggtg ttactgtgat gtgcttgatg aatgcaagtg
     9841 cagcagcgga tcgagcaatg gaaaagaaag gaaagtgaca agatgaattt cggacaaaat
     9901 ttatgtgcga ctcgttcctt agtgaaaagc taaggcacta ataaaagaac gaaaaagttt
     9961 ggttattagg agaactgata atctgacagg tggaatgatg aagtaacgct ctgaaacgcc
    10021 tgccctgagc ctccgacaag cattgtatga ggaaactcag aatgtttgaa gctcggtaaa
    10081 gacggctgaa agataccgta atagttgatg aacatatatc ttctatcgaa aactatccag
    10141 aggtgggtag cgtatccaac aggtcggggg tctataaaat atctatggtt agaatgttct
    10201 ataaggaact gacgaaacag cgaatgtacg ggtctaaatt tgaactttgt agaaatgcaa
    10261 aggtacgaga aatcgtagct ggtgtggcaa agtaagaata taggttatga aatgccatat
    10321 aacgttacag gcggtatcaa gccagcaggc ttatagctag tgacctaagg atatatatgt
    10381 atagataaga ttatcggaac gaggaaaggt attgggttct catcaagaga ataaactgac
    10441 gaagaacaat aagcagttga actaatgctg aaaagcagag ggcgaaccta agaaattctt
    10501 gtaatgagaa tggaggaatg ccctcaagtc gtgtatatat tagaaaagta ttatgttgtt
    10561 tcaataagga ttacgagtaa gataaaaaag ctcactcaat ttcaagtaaa ggagtgaagc
    10621 cttatgccta aaaataaaaa tgaaacattg tgtgtagaag atttaagaca cgctgaatat
    10681 tacgaaatgc aaaacacctt tgatgattta tatgccaaaa gtaagaatgg agatattttc
    10741 actcatctta tggatattat cctatcgaga gaaaatatat tactggcata tcgaaatatc
    10801 aaagcaaatg caggcagtaa aacagcagga acagacggta caataattaa agatattggc
    10861 aaattgccag cagaaacagt tgtcaaaaaa gtaagataca ttgttgcagg cagtccacat
    10921 gggtatcgac ctaagccagt gaggcgaaaa gaaataccaa aaccaaatgg taaaaccaga
    10981 cctctaggta ttccttgtat gtgggacagg cttatacaac aatgtataaa acaagtatta
    11041 gaaccaatct gcgaagctaa atttagtgaa aacagctatg gttttagacc taataggtca
    11101 gttgaaaatg caataaaagc aacctataac cgcctacaaa tcagccagct acattatgtg
    11161 attgaatttg atattaaggg tttctttgac aacgtgaacc attccaagct gataaaacag
    11221 atatgggcta tgggaatacg ggacaagcac ttgatattca ttttaaaaag gatattaaaa
    11281 gctccaatca aaatgactaa cggtacaata acatatccag agaaaggaac tccgcaaggg
    11341 ggaataatat ccccactact agcaaacatt gttcttaatg agttagacca ttgggtagaa
    11401 agccaatggc aggaaaatcc tgttacaaaa aactatgtgg ttcatatcaa taaaagcggt
    11461 agcccatgta aaagcaacgc ttacaaagaa atgaagaaaa caaagttaaa ggaaatgtat
    11521 atggttcgtt atgcagatga ttttagagtt ttctgccgct acaaggaaag tgctgaaaaa
    11581 gccaaaatag caatcactca atggattgaa caacgattaa aattggaagt atcacaagaa
    11641 aaaacaagaa ttgtaaatgt tagaaaaaga tactccgact ttcttggttt caagattaaa
    11701 atgataccga gaagaaagaa gttagttgtt aaatcacata taagtgataa acaattcaaa
    11761 aaccaaaaag acaagctggt atcacaggca aagaaaattg cttctccctc aaaagggaaa
    11821 acggaattag aagaaacaag actttataat tcaatggtat taggtatgca aaattattat
    11881 cagattgcaa cgaatgtaaa tctggattgt atgaaactga atagagctgt aatggctatc
    11941 ttcactaaca gattaggtac aaacaaaagg ggcagattga gaaaaacagg cagacaactt
    12001 acgacaaagg aggcaacaag atatggtaaa tctaaaatgc ttcggtatgt tgctggcgag
    12061 ggcgaaccta ttttccctgt tggatatatc caacacagaa aaccaatggc aaaggtgtat
    12121 ttagcaaatt gctacactct agagggaaga aaatttatcc acaccaatct aagtgttgat
    12181 aaattactga tgtaccagtt aatgaaatta tctttggaaa atcgcacaat agaatacgca
    12241 gacaatcgaa tttcgctatt ttctgctcaa catggaaaat gtgctataac tctggaagaa
    12301 tttcaacaag ctaatgaaat acattgtcat catattgtac caactggagc tggtggaaat
    12361 gacgattata aaaatcttat tttggtaaaa gaggcggtac accgtctaat acacgctaaa
    12421 acagaagaaa caatacaaaa atatatagct ctattgaagc taaataaagt ccagctcata
    12481 agactaaata aatatagagt attagctggc aatcaagaat taaacttgat ataggtctac
    12541 tacataaata cttttcaata taaacacgat ggaacgcgga gtgcggtgaa agtcgcacgc
    12601 tccgtgtgaa tggggggaaa agtttgagat atttataata tcaggaactt acctatccat
    12661 atatcaatgg tttttaacca atgcccaatc cttagtctta ctggcaattg tggtcattgg
    12721 tttgttttta ggatttaagc gggagttttc caaactgatt ggctttttgg tgattgcttt
    12781 gattgcggtc ggattggtct ttaatgcttc tggggtgaag gatgtcttac tcagtttgtt
    12841 taaccgaatt gtcggtgcgt aagggggaag cacgatggaa cagatgcgtg tttatattgc
    12901 gaacctcggg aaatataacg aaggtgaatt ggtgggggcg tggtttaccc cacctgtcga
    12961 ttttgatgaa gtgaaagaac gaattgggtt aaatgacgaa tacgaggaat atgcaattca
    13021 tgattatgaa cttccctttg agattgatga gtatacgcca attgaggaaa ttaatcggtt
    13081 gtgtggctta gcggaggaac ttgaaggaac gccagttggt gaagttgctt ctgagattca
    13141 acatgctttc ttcaattctt ttgaggagat ggtagagcat gtggatgata ttatctatta
    13201 tccagattgc gatgatatga gtgatgtagc atatttcttg attgtagaag ctggtgattt
    13261 tggagaagta ccagaacact tgaagacgta tattgatttt gatgcgtgtg gtagagactt
    13321 ggagattgga ggcaattatt tggtaacgag tcgaggtgtt tttgagtatc catattaaca
    13381 aaattattga agggtagttg atttctaaac tatccttctt tttaaaggaa tgatttgatg
    13441 aagaaaataa aaagctacac cagtatttgg gcagttgaaa aggtcattta tgcaattaat
    13501 gactttcaac tgccttttcc tgtgactttt aaccagatgg cttggtttgt tttatccttg
    13561 ctgtttgtca tcgtatttgc ccatgtgcct ccactatcta tgatagaggg ggccttttta
    13621 aaatacttag gtataccggt ggcagttact tggttcatgt cgcaaaaaac gtttgacggt
    13681 aagaaaccat tgggattttt acgatctttt attagctatc acttacgatt taaggtcact
    13741 ttcgcaggaa agaaagtcaa agaacagaag aaacgatggg acgaacccat tactctggta
    13801 aggagtgtga actatgtacc cgattaagta tattgaaaat aatcttgtct ttaatcaaga
    13861 gggagaatgt tttgcctact atgagttaat accttacaat tactcctttt tgtcacctga
    13921 acagaaatat caagtccacg ataactttcg tcaattgatt gcgcaaaatc gtgaaggcaa
    13981 gattcatgcc ttacaaattg ccacagaaag tagtattcgt gccacgcaag aacgatcaaa
    14041 aaaggaaatt accgggcgat taacagaagt agcgaaacaa cgaattgatt tacagacaga
    14101 ggcattagta tccatgattg gcgatagtca gattgattat cgctttttta ttggcttcaa
    14161 actgattgcg acagatgaag aggtcaatct aaagaatctc aagaaatctt tcttctccgg
    14221 ctttcaagaa tttgtctatg gagtgaatca tcatttgatg ggcgattttg tttccttatc
    14281 caatgaagaa attcgccgat ataccaagct tgaaaaacta atggaaagta aattggctcg
    14341 aagatttaaa gtcagacgag tcacaccgag tgatctgatg tatttgattg aacatatcta
    14401 tggcgaaaag ggtacaccat ttgaagaata tgaatttcag ctaccaaaga aaaagctgaa
    14461 gtcggaaact ttggtgaaac gttacgactt attacgtccc agtcgttgtt tgattgaaga
    14521 aaagccccgg tacttatgca tggaacatga gaatcatgaa tcatatgtag cgtacttaac
    14581 aattaataca attgtggggg aaatggagtt tccctcctca gaacttttct attaccagca
    14641 acaacaattt acattcccaa ttgacactag tatgaatgtg gagattgtga cgaacaaaaa
    14701 ggcacttgct accgttcgca ataagaaaaa ggaattaaaa gacttggaca accatgccta
    14761 tcaatctgac aatgaaacaa attccaatgt cttagatgca ttggattccg tagatgaatt
    14821 ggaaactacc ttggatcagt ccaaagaatc catgtacaaa ctgagctatg tggttcgagt
    14881 aagtgccgaa tcagtagatg aactaaagcg ccggtgtgat gaagtgctag atttttatga
    14941 tgataccaat gtaaaactag tccggccttt tggcgatatg atggggctac atgaagaatt
    15001 cttaccttct agtaagcgat acatgaatga ttatatccag tatgttacgt ctgattttct
    15061 cgccggactt ggttttggcg ccactcagat gttaggggag ttggaaggca tttactttgg
    15121 ttataacgta gatacggggc gcaatgttta cctgaaaccg gcattggcct cacaaggggt
    15181 caagggttca gttaccaatg cgctagcagc ggcattcctc ggttcactcg gtggcggaaa
    15241 gtcctttagt aacaatctct tagtttatta cgccgtcctt tttggtggac aagctgtgat
    15301 tgtcgatcca aaagg
//
LOCUS       DAHHPA010000068        15028 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.069, whole genome shotgun sequence.
ACCESSION   DAHHPA010000068 DAHHPA010000000
VERSION     DAHHPA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.069"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            282..2021
                     /gene="yfcC"
                     /locus_tag="NBH55_001974"
     CDS             282..2021
                     /gene="yfcC"
                     /locus_tag="NBH55_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCD1509953.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2178..3362
                     /gene="iadA"
                     /locus_tag="NBH55_001975"
     CDS             2178..3362
                     /gene="iadA"
                     /locus_tag="NBH55_001975"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCD1509954.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3473..3676
                     /locus_tag="NBH55_001976"
     CDS             3473..3676
                     /locus_tag="NBH55_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD1509955.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3777..4439)
                     /locus_tag="NBH55_001977"
     CDS             complement(3777..4439)
                     /locus_tag="NBH55_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509956.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4452..5954)
                     /locus_tag="NBH55_001978"
     CDS             complement(4452..5954)
                     /locus_tag="NBH55_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1509957.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6502..8181
                     /locus_tag="NBH55_001979"
     CDS             6502..8181
                     /locus_tag="NBH55_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD1509958.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8308..8787
                     /locus_tag="NBH55_001980"
     CDS             8308..8787
                     /locus_tag="NBH55_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCD1509959.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9006..9686
                     /locus_tag="NBH55_001981"
     CDS             9006..9686
                     /locus_tag="NBH55_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1509960.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLIDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9694..11025
                     /locus_tag="NBH55_001982"
     CDS             9694..11025
                     /locus_tag="NBH55_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD1509961.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11040..12002
                     /locus_tag="NBH55_001983"
     CDS             11040..12002
                     /locus_tag="NBH55_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCD1509962.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12113..12451
                     /locus_tag="NBH55_001984"
     CDS             12113..12451
                     /locus_tag="NBH55_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCD1509963.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12530..13237
                     /locus_tag="NBH55_001985"
     CDS             12530..13237
                     /locus_tag="NBH55_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCD1509964.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13340..14821)
                     /locus_tag="NBH55_001986"
     CDS             complement(13340..14821)
                     /locus_tag="NBH55_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1509965.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4801 a         2564 c         3203 g         4460 t
ORIGIN      
        1 gaaattcaat ttttcaatga ttgtaaaatg aaaaaaacag gacgagattt tctctaatct
       61 aaaaataaaa aaagaatttt aaggaaaaac agagaaataa aatacttaat ataaatggaa
      121 agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag tgattttcta
      181 ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt atccattttt
      241 tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa acaaattctt
      301 caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat gtcattattt
      361 ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga aagttcagca
      421 cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact gttttacgtc
      481 aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat caattagaag
      541 aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa
      601 aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa atctccttga
      661 cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct aaaaaattgc
      721 ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc ttaaattttg
      781 tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc gctgcattga
      841 tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc gacgcaggta
      901 tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg ctattattct
      961 ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt ccattctcaa
     1021 tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca gtaaccgtga
     1081 cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt agcgtagcgg
     1141 tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga ttgattatgt
     1201 gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa aaaatccgta
     1261 aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct catatcaaaa
     1321 agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta ttagagatgt
     1381 tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac attccagaaa
     1441 tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt cttttcaaat
     1501 tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca gctgatttag
     1561 ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg ggaggatctg
     1621 atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg
     1681 gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg
     1741 ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct ccactggcag
     1801 atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt tcaggtttta
     1861 tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt gcacggatcg
     1921 aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt gttttaggaa
     1981 ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga cctgtaaata
     2041 aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg gtaaaatcaa
     2101 aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag ttatacttaa
     2161 agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca ccagattact
     2221 taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa gaacagctag
     2281 aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa gtactaaccc
     2341 cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc ggttatcaaa
     2401 atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg acagttgtcg
     2461 gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc aaagcgaaag
     2521 gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga ttgccggtaa
     2581 aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt atcggaatcg
     2641 gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag ttcgctcgtg
     2701 cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc atcaatgtcc
     2761 acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa gaaacagaaa
     2821 ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta tttgaagctt
     2881 gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa gatcctgatt
     2941 tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga ttgatcgagg
     3001 aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc ttgccttatt
     3061 ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca ttgctagtag
     3121 ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta cgagctataa
     3181 ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg
     3241 atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt atcgctaaag
     3301 gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa aaaagttttt
     3361 aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg
     3421 ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca caatggcaga
     3481 tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa aagaagcata
     3541 cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat cactaggtgc
     3601 tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa
     3661 agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa aaaatccgaa
     3721 ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc tgttttttaa
     3781 acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca gctttttgct
     3841 acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc gatttcttgg
     3901 aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg ctcatccgct
     3961 acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg ctgctgacct
     4021 cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac acgagccagt
     4081 cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc cattgctgct
     4141 atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa aataatcgag
     4201 acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc ttgatagtat
     4261 acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt cgtttttcca
     4321 gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag ttctacatct
     4381 ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt ccgtaacatc
     4441 aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat ccctatagaa
     4501 gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag tcccaattgt
     4561 gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa tggattattc
     4621 gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc tggcatttgt
     4681 ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc aagtagctgc
     4741 tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa tgcaagaatc
     4801 atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga ttctcctaaa
     4861 gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat gatcagagta
     4921 agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa acttgccacg
     4981 ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa gctaaatgta
     5041 tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc tggatcagat
     5101 agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag ttcatttgca
     5161 aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt catacccata
     5221 ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata agtggtatct
     5281 tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt tgtttctgct
     5341 agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat tccttcacca
     5401 tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc tgacacacca
     5461 ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc agcaggttca
     5521 ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt gatggcagtt
     5581 attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt tacattatct
     5641 aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa actgcctgga
     5701 tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt tttaaatgaa
     5761 gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc ttgattcgct
     5821 gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag aatggctgtg
     5881 aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt ccatgcacgt
     5941 ttcataaaat tcatattttg catccctcct agtagatgca tttgataata gacaacctat
     6001 cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt tctgtatata
     6061 taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac
     6121 cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac agagggattt
     6181 ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat ttgttgtata
     6241 ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt cgcaaaagat
     6301 ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca gaacgcggat
     6361 gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt tgcatatgct
     6421 tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta aacttggatg
     6481 tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg cggcgttcgt
     6541 gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat tttagattgt
     6601 gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc tgattttacg
     6661 tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg acatgcagat
     6721 gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt cgggacagag
     6781 ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa aggatttaag
     6841 gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt agtgagcttt
     6901 ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac aaaagaaggt
     6961 agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca
     7021 gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact aagtgaatcg
     7081 gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga tgaagtattt
     7141 gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa
     7201 cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt cttgactggt
     7261 caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca attaccaagt
     7321 gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga actgatcatt
     7381 ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc aaacggtaca
     7441 caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac gccaagcatc
     7501 gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg tggaacagtg
     7561 aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga cttgcaatta
     7621 atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta tcgtgaatta
     7681 gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat ctatattact
     7741 ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg aactacaagt
     7801 gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat tgttttacgt
     7861 gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat cagtcggcga
     7921 gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta cgtaaaagct
     7981 agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga gaaacattta
     8041 gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca attaggccgt
     8101 tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat ggaagctact
     8161 caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata gagtgctcgt
     8221 cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc gctttataat
     8281 aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga acgttattta
     8341 gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga agaatctttc
     8401 acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac tctcgtagtg
     8461 aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca cttagattac
     8521 aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat ggattttgta
     8581 ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa actcgcacat
     8641 cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt aattgtgtca
     8701 agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa gatcgtccat
     8761 cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca gagcagagac
     8821 ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc tgttacaatt
     8881 gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta tattttttca
     8941 ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt
     9001 aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg gaaaatggcc
     9061 aacgcgtgat gattgacccg tttattacag gaaatccatt gatagactta actgcagaag
     9121 aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata ggagatatga
     9181 ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt gctgattatg
     9241 cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat gcgtttccat
     9301 tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta gacggtatcg
     9361 ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa acgatttatc
     9421 atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa ttcgatattg
     9481 atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat gcagcaaagg
     9541 ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact ttccctgtca
     9601 ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga aaaataatga
     9661 gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga tttgatttta
     9721 gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat cgcaaaagaa
     9781 ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta
     9841 gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa aaaacatatt
     9901 gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt aggcggggcg
     9961 gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa acatgccatg
    10021 gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga agtccaaaaa
    10081 ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac gactgctgag
    10141 attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta tgatacattt
    10201 acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg
    10261 cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca gacagatacg
    10321 atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac
    10381 cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa atcgccaaac
    10441 cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt
    10501 gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt cgtttccgat
    10561 gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat gcagcttgtc
    10621 cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa tatccatacg
    10681 attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt cgtagtagca
    10741 ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga aatcatttct
    10801 gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg
    10861 aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg tccacgtgta
    10921 ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga aaacaccatc
    10981 gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa
    11041 tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc attatccaca
    11101 ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc gaactattac
    11161 gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa gggcttcgct
    11221 ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta gtcatcgtga
    11281 ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca gaaaaactga
    11341 tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa
    11401 aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct gaatctttaa
    11461 agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat acagggcgct
    11521 ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg agaaattatc
    11581 catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac
    11641 tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga agaataatcc
    11701 ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct tcgatcgttt
    11761 cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag cagccttcag
    11821 ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg gctaaacgcc
    11881 atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag
    11941 aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga aaaacagaat
    12001 gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc ttttcaagat
    12061 cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga agatgacatt
    12121 accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct tgttgatttg
    12181 cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag aagacggatt
    12241 agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga cagtgattaa
    12301 agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat
    12361 ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg gaccgatgaa
    12421 actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt cacattcttt
    12481 tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta
    12541 tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat tagttatttc
    12601 cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa
    12661 aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac agataaataa
    12721 tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag gtaataagat
    12781 gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc
    12841 tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga agattcgtgc
    12901 agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga ttatggtagg
    12961 tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt tttctttagc
    13021 attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc gagcaatcca
    13081 aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa gacacgtatt
    13141 atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc agctgttgcg
    13201 cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg tgaaatcagc
    13261 gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt tggaataaga
    13321 ggatgtgaca agaattttgt cacatcctct ttgttttgtt ctcataacct tttttcatat
    13381 acagttcatg aaccgctttt attaacttgc caaataacac tgtattgtgc gttgtatcca
    13441 gatagaaagt ctccgtttca tctgaaattt tctcatgctg atctgtaatg atcaaatcag
    13501 catgttctga atatgcggta attttaacag catctttgcg aaataactgc tgcaaatggc
    13561 tttgaagatg ataatttcct gtaaactgtt tggttatctg caaatggatg agtaactgct
    13621 ctttcgtttc tgtttgtagt aagtgttgga tcaattgact aaggtattga ctgatttggt
    13681 ttctttcccc attttttgaa aaaattgtat cgattttttc tcttaatttc ttttcctgta
    13741 taaaagaact agaatgattg gacgtggctt ctggaaagca aagtgtagaa aaactactgc
    13801 tggattcacc caacagttcg aaaaaaacaa ctgccaacac aagaaaataa aaataaattg
    13861 gtattttatt ttctaaatgg ttgagaggaa actgttgagt taagtaagtc atcaattgac
    13921 cgacttgtcg acaaataagg tttttgcttg tggaaaataa aaaaccaatc tcctgttttt
    13981 gtcgaaaaga gatttgattt ggaaaaaaac aatagaaaaa ataattaaag taggctctct
    14041 ctgtttctgt ctcttcacaa gggcgactca ctgaaaaaga agtaaagtat tctgaataat
    14101 catgtgtatg ctgcaataag tccatcaatt cagtgatttc agaagaagtt tttggaaaat
    14161 atgcctgttg tttgtttcgt aatatgaata aagttaacag caagtagata tttttttctt
    14221 gttttgaaat tttttctatt tgctcatctg tactgttcat ttttagtgca tttaattcaa
    14281 gtgaggaaaa tggccaatcg ttacctccaa atgtatctga aagaaaaaag taaagataga
    14341 gacggataga taactcattt ccttcaagag tacagcaatt ttttacagat aggatccgta
    14401 aatggaattt ttttaaggaa tgattgagct gttttgtcag tttttcgaga taggagggcg
    14461 atatataaag ttgttcaagt aaatattttt ttggtacagc agtttgtgga taattgatta
    14521 gtaacatcaa atagttaaaa cgattggatt cttttaggta gcataatttt aacttagaaa
    14581 aaacagctaa ctttttagtt tgatcggttt ctttggtttg gatctctcct ttatgattag
    14641 aataaataaa ttcatcaatc aataaaaatt gcttgagtga tacatttatt tttcggatca
    14701 gttctttgat tgtccgtttt ggaatatgta agtcattgga taattcttgt aaagaacaat
    14761 tatttttttg aatgaccatt tgtagaacaa gtaactcttt ttttcttgct tcaattagca
    14821 taaatacctc ctttttattc tgtttttgta tatagttaga caaaaactgt gagtggaaag
    14881 tcatttcagt ctgtgttatt ctcacagagt gtcgccttct tttttatata acgttattta
    14941 atttttttgt taccttgttt attctattgt ttatttccta gggtattttg tcaagatatt
    15001 cttgttttta aggcggaaaa attgattt
//
LOCUS       DAHHPA010000069        15021 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.070, whole genome shotgun sequence.
ACCESSION   DAHHPA010000069 DAHHPA010000000
VERSION     DAHHPA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.070"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="NBH55_001987"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="NBH55_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCD1509966.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="NBH55_001988"
     CDS             complement(322..891)
                     /locus_tag="NBH55_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCD1509967.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="NBH55_001989"
     CDS             1077..1979
                     /locus_tag="NBH55_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1509968.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="NBH55_001990"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="NBH55_001990"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCD1509969.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="NBH55_001991"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="NBH55_001991"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCD1509970.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="NBH55_001992"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="NBH55_001992"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCD1509971.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="NBH55_001993"
     CDS             8547..8732
                     /locus_tag="NBH55_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCD1509972.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="NBH55_001994"
     CDS             complement(8794..9990)
                     /locus_tag="NBH55_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCD1509973.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="NBH55_001995"
     CDS             complement(10160..11596)
                     /locus_tag="NBH55_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCD1509974.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTDILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="NBH55_001996"
     CDS             11889..12698
                     /locus_tag="NBH55_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1509975.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..14881)
                     /locus_tag="NBH55_001997"
     CDS             complement(12884..14881)
                     /locus_tag="NBH55_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCD1509976.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4385 a         3181 c         2663 g         4792 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatgtctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcggg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag
    15001 aagtatttaa tatttttttc a
//
LOCUS       DAHHPA010000070        14622 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.071, whole genome shotgun sequence.
ACCESSION   DAHHPA010000070 DAHHPA010000000
VERSION     DAHHPA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.071"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(177..743)
                     /locus_tag="NBH55_001998"
     CDS             complement(177..743)
                     /locus_tag="NBH55_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCD1509977.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(743..1699)
                     /locus_tag="NBH55_001999"
     CDS             complement(743..1699)
                     /locus_tag="NBH55_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCD1509978.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1699..2355)
                     /locus_tag="NBH55_002000"
     CDS             complement(1699..2355)
                     /locus_tag="NBH55_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD1509979.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2725..4743
                     /locus_tag="NBH55_002001"
     CDS             2725..4743
                     /locus_tag="NBH55_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1509980.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4813..5019
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5170..7584
                     /locus_tag="NBH55_002002"
     CDS             5170..7584
                     /locus_tag="NBH55_002002"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCD1509981.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7932..9485
                     /locus_tag="NBH55_002003"
     CDS             7932..9485
                     /locus_tag="NBH55_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCD1509982.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9866..11650)
                     /locus_tag="NBH55_002004"
     CDS             complement(9866..11650)
                     /locus_tag="NBH55_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509983.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11665..13386)
                     /locus_tag="NBH55_002005"
     CDS             complement(11665..13386)
                     /locus_tag="NBH55_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1509984.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13486..14247
                     /locus_tag="NBH55_002006"
     CDS             13486..14247
                     /locus_tag="NBH55_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1509985.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4749 a         2702 c         2912 g         4259 t
ORIGIN      
        1 aagtaataag aacagccaat caaaaacagc ttctcatgtt tttgatttgg ttggacttat
       61 taccgcagat ataagctttt gagcaccgtg aataaagagg ttatgaaatc aacgctctaa
      121 cctgagtatt agagaaaaaa acgcaaaaca tatcatttat gttttgcgtt ttttatttat
      181 ctttttttct caatacaata taaaataggc ggctggttct tttggttgat aaaccgataa
      241 ttcagcacat tatatgtttc ctgtggcagt ttggaacaaa aagaatcaac catatccagt
      301 tctttttctc caccttcatg accatagtag acgacaagga tcaatcgtcc gcgaggcaca
      361 agtcgtttca atatttcttc catagctgtg cgtgtagtct ctggcatagt gatgattgct
      421 ttatcacttt ttgggagata ccctagattg aaaatccctg ctttgagatt ttgttgttca
      481 gctatcactt ctcctaacgt ttcgtggcct tgtagaaaca atgagacacg ttcttctaac
      541 tgacgctgtt ctagcctttc acgagtagca tcgattgctt gttgctggat atcaaaacta
      601 tagacatgac cagtttttcc gatgtgttct gctagaaaaa ccgtatcgtg gccattgccc
      661 atcgttgcat ctacgacgtg atcccctggt tgcaagattt cttgcagtaa tgtatggctg
      721 aaatgcaaag cagtttgcaa catcagacgc tcaccttttt ccgtacatca tattttcctt
      781 ggaagctatc tctgcgaaga agttcttcat caattgcatt caagacttcc cattttttca
      841 agctccacat tggtccaatc aaggaatccc acggcgcatc ccctgtcaaa cgatggatga
      901 tgatctcttt tggtatcatt tccagctggt cgcaaatgac attgacatag tcaggacggc
      961 tcattaattg aaggcgacct tcatgatagt cccgcaacat tctcgttttg cgcatcagat
     1021 gcatcaaatg gagcttgatt ccttggatat ctgaatctaa aatggtgcgt ctgacatttt
     1081 ctttcatcat ttctaaactt tcgcctggta atccgttgat caaatgcgta caaacacgga
     1141 tattatgttt gcgtaattta gccacgccat ctaaataagt ttgataatca tgtgctcggt
     1201 tgatcagttt tgaagtttct tcaaaagttg tttgcaatcc aagttctacc cataaataca
     1261 tccgctcgtt cagttcagca aggtaatcaa ctacttcatc tggcagacaa tccggtcgtg
     1321 taccgactga taatcctacg acacctggta aattgactac ttgctcaaat cgttctttga
     1381 ttatttcaac tggcgcatgg gtgtttgtaa aattttggaa ataaacgatg tattgttgga
     1441 catgaggcca tttttgatgc atcatgtcga tttctttctg gaattggatt ggaagagggt
     1501 cttcaggtgc aacgatcatg tctcccgaac cagatacact gcaaaaagta caaccgccat
     1561 gggcaacagt cccatctcga ttcggacagt caaatcctcc atcgatcggc accttgaaaa
     1621 ttttcccgcc aaactcattt cgtaaagcgt aattccaaga atgatatctt ttatttcctt
     1681 ctgaataagg aaaagtcatt aaaagtcctt ctttctgtaa gagttatcta tttgttttct
     1741 tttttctcta tacactggat aagtcaggca cagccaagaa agactgagta agtagccgcc
     1801 gatgatatca gtaggaaaat gaacgcccag ataaatgcgg cttatcccga cacttactat
     1861 aataaacatc atcagcaatt gaagcaatgt gcgtaaggtt cttttttgaa caaaagctgg
     1921 aagcagaaat agtaaagaac cgtaaagaat catactgcct gctgcatgtc cactagggaa
     1981 actgtaactt gtttcaacta ctaagtgcgc caatgaagga cgctctcgca tgaaaaatag
     2041 tttgatcacc gggttgatga ttccagcaat tacaataaat ccagaaacga accaaaatgt
     2101 ttctgccctt tttttcttat accacatcca taaagccgca caagcaaata aaatgatgac
     2161 ggtaagagga ttaccgaatt tagtcataaa gagttggtag ctgttcagat caggataaga
     2221 agctctgaca atgcctgtaa accactgatc gaaaccagaa agccacgaca gatggaactt
     2281 tacgatccag cccaaaatga aaaacaggac aagaaagaag ctaccagcca cctgaaatga
     2341 tgttttatat ttcatacaat tcctcctttg aagcaatgac ttattatagc atttttggtc
     2401 cagcgaaaaa aacggtaaag agataaaacc aatgtatgat tcctctttga ataaccaata
     2461 ttcttataaa aaaaataact gtaatcggaa agacattttt tggtaatgca aatgaaatgt
     2521 tagggaaata aaatcagtgt ttttcgaaat ccttttaaac aagaacgtag acgtaggaag
     2581 aacttgaaaa tgaacagaag ttgttcatta cggtaaatta caatatgatt acaaaaaaca
     2641 acaaagctct taaataactt ttaataaata ttaagagaag ttataattat taataataga
     2701 taaattgaag ttggaggata gaagatggaa aacattgcaa gaaaagaacg gagacgttta
     2761 gaacagacca aacgttttcg aaaagcaaaa cgaggggcag caatcgtggg aactgcgatg
     2821 gtaggctgtt ctgtcgctgc cccattagta cagccggttc ctgttcaagc ggatgaaaca
     2881 cctgttcagt tcggcgcaag aatcaataca gcggcattta tcacagaaat tgctacttat
     2941 gctcaaccaa tcgctcaagc caacgatcta tatgcatcag tgatgatcgc acaagcagtt
     3001 gtagaaagcg gttggggcag tagtgcgtta tctcaagcac cttattataa tctttttggg
     3061 ataaaaggca gctatcaagg acaaaccgtc tacatggata cgcttgaatt tttaaataat
     3121 caatgggtaa ataaaaagga gcctttccga caatatcctt cttttgctga atctttcaat
     3181 gacaatgcct atgtacttag aaatacttca tttggcaacg gttattacta tgccggtact
     3241 tggaagagca acacgaaatc ctatacagat gcgacagctt gtttgacagg gcgttatgcg
     3301 actgatcctg gttacgcagg caaattaaat aatatcatcg caacttacgg attgacccaa
     3361 tatgatacac cagctagcgg aaacgcaggc ggtggagcga ctacaggtgg caataatact
     3421 ggaagtgcag gtagtactgg cggttcaagc aattctggta atacaaactc aggttcagca
     3481 acttcgacca cttatactgt ccaatctggt gattctgttt ggggaatctc aaataaattc
     3541 ggtatcacaa tggatcaatt gatccaatgg aacaacatcc aaaacaattt catttatcca
     3601 ggacaaaaac tgacaatcaa aggtagccag gaaaatggct cttcgacgaa taattccggt
     3661 aacaatacca actcttcagg aaatgctggt acatcaaacg gaggacaaac aactggtgcg
     3721 aaatacactg ttcaatctgg tgattctgtt tggaagatct caaatgatca cggaattacg
     3781 atggctcaat tgattgaatg gaacaatatc aagaacaatt tcgtttatcc aggacaacag
     3841 ctaatcgtat caaatggcgg ttcttctaat gctggagcaa atactggctc aaatactggg
     3901 aatacaagtg gctctagttc gtccagctca agttcttcta atacagcttc aggaacgaaa
     3961 tatacagtta aagccggaga ctctgtttgg agcgtctcaa ataaatacgg aattacgatg
     4021 gctcagctaa tccaatggaa caatatccaa aataacttta tctacccagg acaacaactg
     4081 actgtttcaa acagtggatc aacaaacagc acgtcaggta atacaggttc gtcaaccaat
     4141 acaaataaca gcagttcagt taatactggc agcagttcgt ctcaaggcac ttataccgtg
     4201 aaagcaggtg aatctgtttg gagcgtggca aataaaaacg gcatcacgat ggatcagtta
     4261 atcgaatgga ataatatcaa aaacaacttc atttatccag gacagcaatt gatcgttaaa
     4321 ggtggaactt cggcaaatac aaatacaggt tcaactactt ctgcagcgaa accaaataca
     4381 ccgaatacgt ctgctacgac aagtacatca tccactggta atacgatgta taccgtgaaa
     4441 gcgggcgagt cagtttggag cgtagcgaac aaacatcata tcacgatgga tcagttaatc
     4501 gaatggaata atatcaaaaa taatttcatc tatccaggac agcaagtgat tgtgaagaaa
     4561 ggttcatctc aatcaacaaa ccaacaatca gttactggtt cgaaaaccta tactgtgaaa
     4621 tctggagaat ctgtttggag tgttgctgac agtcatggaa ttacaatggc tcaactaatc
     4681 gaatggaaca atattaagaa caactttatt tatccaggtc aaacgttgat tgtagctaaa
     4741 taaaatagct gttcgaagaa ataacgattg aattccacaa aaatatttgt tactataagg
     4801 ctacttaaat atgacaacac ttgaaaagga agtagtaaaa agactgaagt ttcagagagc
     4861 gcatggctgg tgagaatgtg cacggatgct tttgaactcg cctttgagtt gcccttgctg
     4921 aatacccgag taagcaagcg gcggtgacgg ccgttacaac gttcgaggcc ttttcactaa
     4981 ggtaaaatta ggtggtaccg cgtagattac gtcctataaa aagagcaatc tttttatagg
     5041 atttttttat ttcttttgtg aagtttgaga aaacaaagca aaaggaaata aaaataaaca
     5101 gattctgccg gttttttcgt tatatttata atagaaacaa gtgttttcaa tagaacagga
     5161 ggacagcaaa tgagttacaa tcataaagaa attgagaaaa aatggcaaaa atactgggca
     5221 aaaacgaata ctttcaacac ccatgatgat cctgaaaagc caaaatttta cgcactggat
     5281 atgtttccct atccatccgg tcaagggcta catgtagggc atcctgaagg ttatacggca
     5341 acggatatcc tttcacgtgt gaaaagaagt caaggataca acgtgctgca tccaatgggg
     5401 tgggatgcat tcggtctgcc tgctgaacaa tatgcgctag atacaggaaa cgatccagct
     5461 gagtttaccc aaaaaaatat cgaaacattc cgacgccaaa tcaattcttt aggatttagt
     5521 tatgactgga atcgcgaagt gaatacaact gatccggagt attataaatg gacacaatgg
     5581 atctttacga agctttatga aaaaggtcta gcttatgaag cagaagtagc ggtaaactgg
     5641 gtacctgaat taggaacagt tatttcaaat gaagaagtga tcgatgggaa aagtgaacgt
     5701 ggtggctatg atgtagtcag aaaaccaatg cgccaatgga tgctgaaaat cactgcttat
     5761 gctgatcgct tattggatga tctagaatta gttgattggc cagaaagcat taaagaaatg
     5821 caaagaaatt ggatcggtcg ttcagtagga gcaaatattg aatttaaagt ggctggtacg
     5881 gataaatcct ataccgtatt tacgacacga ccagatactc tattcggtgc aacttactcg
     5941 gttcttgcac ctgaactaga tcttgttaga gaaattacga cccctgaaca aaaagctgct
     6001 gtagaagctt atatcgaaga gacagctaaa aaatcagatc taaagagaac ggatcttgca
     6061 aaagaaaaaa caggagtatt tactggtgct tatgcaatca atccagttaa tggcaaggaa
     6121 atcccaatct ggatcgccga ttatgtatta tcttcatacg gcacaggagc catcatggct
     6181 gttccagcac atgatgaacg tgactttgaa ttcgctcaaa ctttcggact agaaattctt
     6241 ccagttatcg aaggtggaga tgttgaaaaa gctgcttaca cagaagatgg tacgcacatc
     6301 aactctgaat ttctagacgg catgaacaaa caagaagcaa tcgataaaat gaatgaatgg
     6361 ctagaagaaa atggcgtagg gaaaaaagaa gtcagctatc gcttacgtga ctggctattc
     6421 tcccgtcaac gttactgggg tgaaccaatc ccaatcatcc attgggagga cggaacagtc
     6481 acagctgtcc cagaagaaga gctaccatta cggttgccta agacaaaaaa catcaagcca
     6541 agcggtactg gagaatctcc attagcaaat atcgaggagt gggtcaatgt cgttgatcct
     6601 gtaacaggta aaaaaggccg tcgcgaaacg aatacaatgc cacaatgggc aggaagctca
     6661 tggtattacc tgcgttatat cgaccctcat aacaaaaaag aactagcaga ttatgaaaag
     6721 ttgaagcgtt ggctaccagt tgacatctac attggtggag cagaacatgc ggtacttcat
     6781 ttactatatg cacgattctg gcataaattc ttatacgata tcggtgttgt tcctacaaaa
     6841 gaaccattcc aaaaactata caatcaagga atgatcttag gcgaaaacaa cgaaaaaatg
     6901 tcaaaatctc gtggtaatgt agtcaatcct gatgatgtgg tcgaaactta cggtgcagat
     6961 acactgcgca tgtacgaaat gttcatgggg ccattagatg cttcgattgc ttggagcgaa
     7021 aatggcttag aaggaagccg taaattcttg gatcgtgtat ggcgtttgat cgttgatgag
     7081 aacaataaaa tgcgtgaccg tatcactaca ttgaatgatg gcaagcttga taaagtgtat
     7141 catcaaacag tgaaaaaagt gacagaagac tatgaaaatc ttcatttcaa tacagcaatc
     7201 tcccaattga tggtctttat caatgaagcc tataaagtgg acgctctgcc ttatgaatat
     7261 atcgaaggat ttgttcaact actcgcaccg attgctcctc acatgggtga agaactatgg
     7321 gcaatccttg gaaatgatgg tggtatttct tacgcaccat ggccaacata cgatgaagca
     7381 gcacttgttg aagacgaagt agaagttgtt ttccaagtaa acggtaaagt tcgtgcgaaa
     7441 agcaatgttc cccgagactt aggaaaagat gagctagaaa aagcagcatt agccaatgaa
     7501 atagtgcaag aatatattga aggcaaaaca gtcagaaaag ttattgcggt accaaacaag
     7561 ttagtcaata tcgttgctaa ttaattggct ctttgtcaat aaggactgat gagttgtgca
     7621 aaatcaaatc tgggccagaa tgaacttcat tctgtctcag attttttgtt atttcaaatt
     7681 gattaattga ttgatcaaaa aggttttgat tttcatgttc tcaaacacgt tcaatgtctt
     7741 tttcttctga atggtcttgt aagagatctc tgtaatcatc aaatttttgt ctattaatct
     7801 tagtattttt tgatgtataa actacctgaa attgagagta cataccaaat tcataaattt
     7861 tttattagtg ggactagtga ttgtatggat tttgacgcat aatgaatgaa gtatgagaaa
     7921 aggagagaca aatggatcaa gaactattta ttatcgaagt attatcagct aaacaaaaaa
     7981 ttatcgagaa agcgaaaaaa agagcgaaaa gaataatgtt caagctttta agcttagtat
     8041 ttttctctgg tttgcttttt ttcggagctg tatcgttcct atatcgccat atgagtgcag
     8101 tagtccgttc agctgaaggg atgcaaaaat taaataattt gtatcacttt tcgtggggag
     8161 ttggcggaat aacaattgtt gtgcttattg tttttcttct acttatttgg ctccgttacc
     8221 atcaattgtc gcgtcatttc gatcaatggc ttagtcaaag agcacaacag tttttacata
     8281 ataaaccaac ttctcaagat gatgactatc tttattttga gaagacttcg acgagtcaag
     8341 cttttcggat acaaaaaagt gattgccagt tattgctgag atcacttgat gatttaccta
     8401 tctacttagg cgaaagcaaa tcaaaattcg gaatgaacac attccatatt tttttactag
     8461 aaaacgaacc cagatgggaa aaaagccagt cttcgcctgg tggatacctg acaactaaga
     8521 tgagtacatt agtcactggt tttttcctca ttcttttagc tggaacaatc tattatggga
     8581 gatacactca gccaaaaatg tcttctcata taaatgctgc ttctcacatg acaatgaact
     8641 caactaacga tacgcatata caaaagacaa atgaagggtt gttagctgat cctaagcaaa
     8701 aggtaaattt gacaacacaa aagataaatg atctcaaact tgatacagag acgcactctc
     8761 tctatatgac aacagatagt ggtgctaatt gggtatttgt gccgattgat tcgcaatggt
     8821 tgcgttttgg cgaatacacc ctgacaacgg ggctgattcc tgaaggttat tggatggaca
     8881 acacatatga tatttccgcc gatttctcat ggtttatcta ttcttctggg gatgatgtac
     8941 gtctgctgac ttcaagagat aatggtaaaa catggcaaac gtctattatc acccaatcag
     9001 ttcagccgat acgttatcga aaagcggtat tttatggcaa cgatggtcta tttatctgta
     9061 catcagccag tgggatgtct gcagaaactt tgtacattta cactactatg gatggcggaa
     9121 tcacttggat aaaaagtgga agtacaatga tcaatcagcc agtacaaaat gtcagcttcg
     9181 ttacaacttc tttaggtttt gtttcgacac gtgaaagttt gttttataca aacaacggag
     9241 gacgctcatt caaagaatca gttctgtcga ttccggaaaa ctatcagaca ggggggatag
     9301 atctttttca atcaccaaat gaagttactc aaataagcgc gaatacgtta gagacaaaat
     9361 tttatttgtt gaaaaaagga ccaatcgatg cagggaaaat gtttgcctgc ctcttccgtt
     9421 caactgataa cggggaaaca tggcaattcg ttcagcaact atcgcaagta gaacaaggag
     9481 attaaagaag agagattgtg agcctgacgt tcagcaccga gtcacaagaa acaaattcaa
     9541 aaaacagctt ctcatatttg ttgaatagtg taactcgtga ttcggagttg acaagagcaa
     9601 caagaagcga ttttgaagta tagcttctcg tattttacaa gatcgtgtct tattgccgat
     9661 aggttgtctc ttgaacaccg tttattcgta aaaagggatt gtgacaagtc ttttgtcact
     9721 acttaccgaa cgaaactgaa ggagctatcc aaaaagtatc attagtatta ttctttgtgt
     9781 ttctagtggc accactagta catttaaaaa agctatcgat catggactaa tcatacatga
     9841 tcgatagctt tttagataat gtttgttacg ctatctgaaa ttgactatta taaagactgg
     9901 cataaagcgt tggtgattgg agtagttcag aatgtgttcc tttttcgacg atatcgccat
     9961 tcttcattac aaggatcatg tctgcatttt caatcgttga caaacggtgt gcaatcacaa
    10021 aacttgtcct atcttttgtc acagcttcca ttgcttgctg aatcaatgct tctgttcgtg
    10081 tatctacgct agaagttgct tcatctaaaa taacgacagg aggattcgct agaatgactc
    10141 gagcaattgt caataattgt tgctgtcctt gagataacgt gccgttttcg ctagaaatga
    10201 tcgtatcata tccgtctggc agtgttcgaa taaagtgatc acattgtgcg atttcagccg
    10261 cttcgataat ttgttctctt gtggcatcca ttttcccgta ggcgatatta tcagctaccg
    10321 ttccttcaaa cagccaggta ttttgtaaaa ccatcccaaa agaacgacgt agttctccac
    10381 gtgaaagctt ggtaatatca agaccgtcga attcgatctt tcctttattc agttcataaa
    10441 aacgcatcag cagattgact aaagtcgttt ttcctgcacc agttggacca acgatcgcta
    10501 ccgtttgttt tggctttact gaaaaatcga cttgattcat caatatttta tctgatgtgt
    10561 aaccaaactg tacatttttg aaagctattt ttcctttcgg ttgttctaaa agtcttgcat
    10621 ctggaaggtc tggaatttca tctgtttcat ctaatatctc aaatatccta tcgattgctg
    10681 ccattgcgga ttggatcgag ttgatcacat aagaagcggt agaaattggt tcagaaatct
    10741 gattgatgta ctgaagataa gcttgtaaga agccgatcgt cacaccacct gatagaacaa
    10801 gcatagcacc gaataccgca cttatgatga aggcgagttg attaatgaaa cgaatggccg
    10861 gatagatagc acagttcata aattgagcac gtttaaaagc gttgtattgc tttttattta
    10921 cttcgtgtat agcagcttct gcatgagctt gctgattgaa tgttttgacc actagattac
    10981 cagctaagat ttcttccatt cgattattta aaacacccag ttcatactga ttctgttcag
    11041 aaatctgttt gtttttgttc gcaatcttag ttgtaacaat tgaactgcca acgatcagca
    11101 ataagacgag caaagtaagc ttgctttcga tataaaacag catcagacca gcagcagcta
    11161 ttgttacgac agaagtaaaa aattgattga tacctgtcaa taacacttga gaaagttgat
    11221 tgagaccggt agttgtccga ctaatgatgt cacctacttg atgattgtca aaaaacgaca
    11281 tcggtaatgt tttgaatttt tttgtgattt cttttcgcac cctcaatgtg atcttttcgc
    11341 ttaacgctgc cattgtccgt tcctgaataa atgaagtgat gctgcttaag actgaacagc
    11401 cgatcagcaa taagacaggg aacaacaatg cttgtttgat atcactgaaa gaaagctgtc
    11461 caatacctgt ttttcttatt tctgataaga gatcatcgat cccgattccc atgatcaagg
    11521 gcatggctac gactaatagg ttcccgaata aactgcatag aaacaatccg taaaaatatt
    11581 tatgttcagg tttgatcatt ttccaaaaac gtatcaaagc acgcgtgttc aacgtttttt
    11641 tcttttccat cgtatccctt ctttctatat tccttgtgat tttgcaaact cctgataagt
    11701 ttggcaatta gacagtagtt cttcatgagt accttgtcca acgatttttc cttcatctaa
    11761 aacaatgatg ttatctgctt gttggattgt actgaggcgt tgagcaacaa tcacgagagt
    11821 tttctccgac atgtgtttat gaagggctgc tcttaatgct gcatctgttt tatagtctaa
    11881 agcggagaaa ctatcatcga atacatagat ctctgctggt ttgatcaacg ctcgagcaat
    11941 acatatccgc tgtttctggc cccctgaata attagaaccg ccttgagcaa caaagctgtc
    12001 caatccgtca ggaagggtgt ccactagttc ttctaattga gcgatcttga tcgctttaga
    12061 caactcttct gttgaggcat attcatttcc catctgcaag ttgcttaaaa ttgtcccgct
    12121 gaataagaaa gccttctgag ggacataact gatgtgggag cggatctgtt cttgagtcat
    12181 ttgagtaaga ggaataccac caagggttac ttttcctatc gtagggtcac taagtctcaa
    12241 gagaagtttc gccaaactac tttttccaga tccggtcccg ccaacgatcg ctgttgtttt
    12301 tcctttaggt attgtgaaat caatatcttc tagaactgga tcagagattt cagtatattt
    12361 aaatgtgaca tgttcaaaag cagctgcatt tgtctgatca aaggttggtt cagctactgt
    12421 tttgatcgtc tcttcctcaa tttcgatctc tgcttgcagt atttcttcta tccgtcgtaa
    12481 tgaggcaaac gatttaggga taatgaccaa taccatagct gcgatcatca agtaagcaag
    12541 tgtcagcata gagtattcga ctacagcagt cacgacaccg atttgtaatg ctccttctgc
    12601 tacaagattt ccgccaaacc acaaaataga agcataaacg attcccatga gcaaaaaagc
    12661 aattggagtg acccaagcaa atattcggtt cactttgatc atttgattag catagtcagc
    12721 aaagctctta ttcgttcgct tttcttcata tgattgatta tcaaaggcac ggatcatatt
    12781 aatcccagta aagaattctc tcaatgtgat ggtgatccga tccatttttg gctggatact
    12841 caaagataaa ggtgcccctc gtttcatcag tcgataaaca atccatacat aaaaaacaac
    12901 agagatcaat gtaacccatg ttagtaatag agaataacga aacatcatga gaatggtgaa
    12961 aacgcaaata accggtgctg gtaagatcat ttggcaaaat aaaacaatca tttgctggac
    13021 attatcgaca tcattcgtca ttcttgtcaa aagagatcca gaactaaaac gactgatatc
    13081 cttcattgac agctgttgga atttacggaa atatttctcc cgcgtttgga atccaaacct
    13141 agcggaagcc aatgctgaaa agtaactaga ggcaactgca gcgattgttc ctagaaacgc
    13201 tacaagcagc atttcaactc caattttttt tataactgag gcttcgccac ttgctatacc
    13261 tgtgtcgatc agctttgcta ctaattttgg tacacctaat gtaccggcga tttctaaaca
    13321 gatcatcaca aaggtaagga gagcgagtcc tttgtttttc tgtaagaatg tttttaagat
    13381 agacatatta ttcctccttt tttcctgata cttttcgatt ctataccttc aagtaacttg
    13441 aaggtcaata gaaaaaagaa taaactgaaa aagaggtgag aaaaaatgaa aaaaatgtat
    13501 acgattggcc aagtatcgga actatttgat ttgccaaaat caacgatccg ctattgggat
    13561 gaacaaggat taatccgttc aagtagacaa gaagagaatg attatcgatt gtttgatatc
    13621 gatgatattt ttatgcttta tgatatcgtt ttttatagaa aattagatat accgatcaaa
    13681 cagatgaaga atttatatgg aaaaacattg acggagttat atgagacact tgatgaaaca
    13741 gaaagaagaa tccataaaga actggtggtg atgaaacaaa aacaaaaaga gataagggag
    13801 caaaaaaaac agttgaaatt gatgatcgat acaaacgagg aagaatttcc tgtagaagag
    13861 atcccttttg actgtatgat ctcaacggag tttgaagata ttgtggaaat caaaaaattt
    13921 ctacctaact attcaagctt tgggatgatg agtatgccag cttccagttc acagacgatg
    13981 tatggatttt ttatcgaccc tagtgaagta catttattta ctcaagacgt gatttgggag
    14041 aaaaaagata cggctgttta tcgtcgattc ttattgaaat ccgagatgaa ccatgcggaa
    14101 cgtaataata ttttagaaat cagagaaaga atgtatgaaa aaggatggaa aacaggagaa
    14161 gtgatcggcc agtatctatt gacgaatacg gatgaaaaca atatcagaac tgaatattat
    14221 catgcatgga tagaaatgaa aaaataggaa atatgtgttt aagataactc gaaaatgatg
    14281 gttgttttag ttataaagaa aaattttctg gttcgattac gggtttaatg tgggggatta
    14341 ttagtcgtgg atagatagaa ttttagtttt ttagaggaaa tatgtcttta aaaatggaat
    14401 ggttgcaatt cttagcgata tatacgatgt aattgtcctc ttaaaaaagt atggaaaaat
    14461 aattgtattg ggtgttatgt ataattcatg gtgaactgag actaatgaga agaaaaattc
    14521 atattgttat gagtgagatt gtatcagatg agaatgataa attaaatagg tgcataaaag
    14581 cattacactt aaaatacatt gttgcttaca ttattacaat at
//
LOCUS       DAHHPA010000071        14374 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.072, whole genome shotgun sequence.
ACCESSION   DAHHPA010000071 DAHHPA010000000
VERSION     DAHHPA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.072"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            19..400
                     /locus_tag="NBH55_002007"
                     /pseudo
     CDS             19..400
                     /locus_tag="NBH55_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            531..1481
                     /locus_tag="NBH55_002008"
     CDS             531..1481
                     /locus_tag="NBH55_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCD1509986.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1999..2454
                     /locus_tag="NBH55_002009"
     CDS             1999..2454
                     /locus_tag="NBH55_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1509987.1"
                     /translation="MVKQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2795..3397
                     /locus_tag="NBH55_002010"
     CDS             2795..3397
                     /locus_tag="NBH55_002010"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1509988.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3491..3823
                     /locus_tag="NBH55_002011"
     CDS             3491..3823
                     /locus_tag="NBH55_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HCD1509989.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(3974..4953)
                     /locus_tag="NBH55_002012"
                     /pseudo
     CDS             complement(3974..4953)
                     /locus_tag="NBH55_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4958..5725)
                     /locus_tag="NBH55_002013"
     CDS             complement(4958..5725)
                     /locus_tag="NBH55_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1509990.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6215..6640
                     /gene="lacA"
                     /locus_tag="NBH55_002014"
     CDS             6215..6640
                     /gene="lacA"
                     /locus_tag="NBH55_002014"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCD1509991.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6657..7172
                     /gene="lacB"
                     /locus_tag="NBH55_002015"
     CDS             6657..7172
                     /gene="lacB"
                     /locus_tag="NBH55_002015"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCD1509992.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7183..8115
                     /gene="lacC"
                     /locus_tag="NBH55_002016"
     CDS             7183..8115
                     /gene="lacC"
                     /locus_tag="NBH55_002016"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCD1509993.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8118..9098
                     /gene="lacD"
                     /locus_tag="NBH55_002017"
     CDS             8118..9098
                     /gene="lacD"
                     /locus_tag="NBH55_002017"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCD1509994.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9126..9443
                     /locus_tag="NBH55_002018"
     CDS             9126..9443
                     /locus_tag="NBH55_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD1509995.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9443..11149
                     /locus_tag="NBH55_002019"
     CDS             9443..11149
                     /locus_tag="NBH55_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1509996.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11291..12697
                     /gene="lacG"
                     /locus_tag="NBH55_002020"
     CDS             11291..12697
                     /gene="lacG"
                     /locus_tag="NBH55_002020"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCD1509997.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12720..12992
                     /locus_tag="NBH55_002021"
     CDS             12720..12992
                     /locus_tag="NBH55_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HCD1509998.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13013..13912
                     /locus_tag="NBH55_002022"
     CDS             13013..13912
                     /locus_tag="NBH55_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCD1509999.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4682 a         2377 c         2777 g         4538 t
ORIGIN      
        1 atagaaaaga gagataaagt gaagttttta aattttttga actataaata tttaaactgg
       61 gtattatatg gtattacatt attttttatt atttattact tttttaccga tgatgatatt
      121 tttttaattt tagcaatact ttttcttgtt tcatctaatt ttagtgataa cttaattgaa
      181 gttcttacag aaccagaaat tcggtctatg acggatgaag aacaacagat tattaaagct
      241 atgatttcgg aaaataagaa agacgtagta gttatcaaaa agattagaga gtatacaaat
      301 ttaggtgttt tacaagccaa aaaagtatat gaaaaaaaaa aaaagggtct ataattacaa
      361 attagataat acattagaag ctggagacaa gattgtctag ataatgtgtg agataaaatt
      421 tttggcaggt tatcatacat aagacaaaat gtttttaaaa ctgataacgt atttattcca
      481 caacaattac gcatttattt ctgagaaaaa tataaagcag gaggattaca atgctgaaaa
      541 cttattcctt aacaaaagat acattattag tttcgtcaga atcaaatatc aaaaattctt
      601 ctgttgttta tgtttatggt gacgaacgag aatatatcga atcttctgaa cataaatatg
      661 actttaaaat aggcaatata ctaacgtttg atgatcgagt tgcatatgac acgatgatag
      721 ataagaacga tgaaaaaagt ttgctcgtaa gttttttgtt tcccgaggta catgaaggtg
      781 ggcatctaga taccctgacg acatctgttg ttttcttggt atttaaaaat aagttgataa
      841 tttttacaaa acaaaaatta aaattcattc ccgaactctt atccaatatg gttttacgtg
      901 atgtcaatca tttcgtggag gaagtacttt taaagatcat gcaaaaagat ttctatatga
      961 tgcttaacga ccttcgtgga gtcaaggaaa aaattgatga tttagactca gaaatatcaa
     1021 cgtccggttc actcaaacca acgtttggtg atttattgac tcttcaaaaa tacatgatag
     1081 ctttgtcaac aacttataaa gctaaccata aagcgcttga tttcattaaa aaaaatttca
     1141 caactattga cgatattgat tttagtacta aaaaaataat tgatgattta tacgatacaa
     1201 gtgatacaat ggatagaatc attttaagtt acagccaata tttggacaaa ttggaaaata
     1261 tgataaacaa tatgatttcg tatcagctaa acatgattat gaaaacccta accgagatat
     1321 caattgtttt gactattcca gcaattattt ttggcttttg gggaatcaat gtccatgtcc
     1381 cgtttgaaaa gtcttcttat ggtgttttag ccgtattgat tatctctgct atactaagtc
     1441 taatttgctg gcttttgatg cgacgcaaaa catatttgta aagtatttgt gataccctaa
     1501 tatgatttaa agcagaattt ctttgttgac tttttaaaag gctgattcct ttcaataatt
     1561 taatagcttc agcggctgct cagaagtcaa taaattggac tatttttatt cagttactta
     1621 ggcaacattt gagatacgat tgcgattata aaaattgata taccaaccaa tggctgcttg
     1681 gaagactgac atattagcct cgatatcatc gaataactaa atacagaaaa atacaataaa
     1741 tattatcaaa ctagggacta tactagaaaa catcagtaca ttccttaact cagggtctat
     1801 aattagaaaa tcgccccttt gaaaactgat taaaacagcc cccattatct agtgactaga
     1861 taatgggggc tgtttttttg ttaagtgata taataaacca taaaagaagt ttagaaatac
     1921 ggtcctggtc atcaataaat aaacaattta attaattatt tatttaatta tttaatttca
     1981 gaaaggtata catgaatcat ggtgaaacaa attgtcctac ccatcaaaga ctccagcgtc
     2041 ttaaagatgg tacaagatac acttctcgat agtttccgtg ctggtcgccg aaattacacc
     2101 gtttttcaag ttggtaaggc cacgctacta cgtgtgagtg atgtcatgac attaaaaaaa
     2161 tcagatgtct ataatccaga cggctctgtc aaaaatgcaa cctttattca tgataaaaag
     2221 accggtaaag caaacacgtt atatttaaag cctgttcagc aagacttgtt gcaatatcat
     2281 gattggctgg cccaagaaaa tatcaattct gagtggttat ttccctcgac agcccattat
     2341 gatcttcata tcactgagaa gcagtttcat aaggtgatgg cgcatgttgg tgatctctta
     2401 ggaatcaact tggaagacaa agagaaaaaa ttgcgagtca gaaaaccgtt ttgagatatg
     2461 aacagcattt gccttcacgc cttagcgatt ttaaataata gccttgaaag tgattatgga
     2521 aaagccctct acgctcaatg caaaattgac gtagaaacta tttttgatcg attgaaaggt
     2581 gtatttggta tgtgaagaac ccatgtcaga ggcaagaacg ttgcacctac tgacattgga
     2641 atgatgctta tgagcatgaa tctaaccaaa ttatcccttg aggctagaag aaaagcggaa
     2701 gcttttcaac acaaagcagc caaaaataaa aaacgcgacg aagcaatcac atttatgatt
     2761 attcatcgcg ttttttatat ttagagctag ttatttgcta gacccactat ttacagatga
     2821 aacgtttatc acctttggtt ttgggcattt agtggaatta gcagaaccag gtcattatga
     2881 cgaaaagtgg caaaattgga aacttgaatc tttgccgatt tttcctgatc gatacgattt
     2941 tgaagttgca aaagataagg gaaagcagtt taaaattgtt gcagaacttc tcaaaaaggc
     3001 aaatacaatt attgttgcaa cagatagcga cagagaaggt gaaaatatcg cctggtcgat
     3061 tatccataat gcaaatgcct tttcaaaaga taaaacattt aaaagactat ggatcaatag
     3121 cttagaaaaa gatgtgatcc gaagcggttt tcaaaatttg caaccaggaa tgaattacta
     3181 tcccttttat caagaagcgc aaacacgcca aattgccgat tggttgatcg gcatgaacgc
     3241 aagccctttg tatacgttaa atttacaaca gaagggcgta caaggtacat tttcactagg
     3301 acgtgttcaa acgcccacct tataccttat ttttcagcgc caggaagcca tagagaattt
     3361 taaaaaagaa ccttttttcg aggtggaagc taagtaaaac aagggtcatt taagggcgtt
     3421 ctaagcccca cacagcgttt taaaacccaa gaggagcttt ttagcttttg tttcttctaa
     3481 acaagctaaa ataggcaatc aagaggggat aattgctgat gttcaaacca aagagaagaa
     3541 aacgaatagt ccgagttcgt tttctttaag tagtttgcaa tcaaaagtca atcagcttta
     3601 taaagcgaca gcgagccaaa ctttaaaagc tatgcaagga ctgtatgaag caaaattatt
     3661 gagttatcca agaacagata caccatttat tacagagaac gaatttgctt atttaaaagc
     3721 gaattttggc aaatatagcg gttttttagg acttgatctt gaaatgttgt tatcattgat
     3781 aatgatacgt tcaaacaaca gcacccaaat tttgacgaat tagtgaaact ctatgaaaaa
     3841 gacgtagtaa aacatgcaac atcctattct aatcagctgg taaagcttaa ttaggttata
     3901 taagcttgaa aataaagggt ttgtccaata tggacaagcc tttttcttat gttttgccac
     3961 agatttgcca cacttattgt gtaagagtat caaggatagg tataatttga tactttgttt
     4021 ttttagtgat gtgcaaataa atttctgtta cacgactacc tctagaatgc cctacacggt
     4081 cttgaattgc ttctaggggt attccttact ctgctaagaa actaatatgc gtatgacgga
     4141 atatatgggt cgtaatatgt ttattaaaac taagtttatt atcaatatca attcctgctc
     4201 gttttggatt accttttcga tttaattttc cattttcagg tgagttgagg aaattgttaa
     4261 ggacactata atgaagaggt gttccattat agtcgggttt atgacgactt ttggaagtaa
     4321 aaatccatcc attatcttta taatcaggat tgaatctcat atgttggtga tttaactcaa
     4381 tttcctcttt aagaatttct atagcccttt tagataattg gacatttcgg taactttgta
     4441 agttttttac attaccttca tagctatttt ctgcagctcc tccgtgagtg gactgactaa
     4501 tgatgatatt ttgcttttca aaatattgat aggcaccaat attttggagt ttcgactctg
     4561 taagtcaaca gggccattag ttagaatacc tattgggatg cggtttttat gtagagccag
     4621 cagtagttga tgtatctcag gaatcatcgt aatctgatcg agttcatatt catagtcagc
     4681 ttggaagtct agagcttctg tcctagaaat actatcgatg ccaaattctt ccatagtctt
     4741 ctgacagcga aaaattctaa gctgctcaat actgatgagt tttttcgtgt atttaggaaa
     4801 agcaacatca gaccaatatc tgaaacgttt gtaaatgtta tctatttgtt ggatatcaat
     4861 cgttggaaaa catttctcaa cgctacgttt aaaggggagt tgatggtcat aaagagtgtc
     4921 atcaaggtca aaaacaatat ttttataagt cataattcta gtccgctttt aaaatagtag
     4981 tgtactggct aaattcctct aagtgttgag ggctaatctg gttatctgta atgatggtat
     5041 cgagttgatc tagattatag aagttaaaga aatcgtatcg atcgaattta gtactgtcta
     5101 ctaataagaa tttttctaca gcattgttta gggcaagttg ttgaatcaca ccttccttgt
     5161 cactatatgt agcaatagaa ttatgggtaa cagcatttgc actaacaaaa gctttggcaa
     5221 atctaattgc ttttaaattt gtcgaagcca ttgaacctac aaaagctcca gttatttctc
     5281 tatattcacc gccaagaagc aataaatcaa tggtttcgct atcatttaga atcaagaaca
     5341 cagggagact atttgtaatg acacgaattt tataaccttt acgacctttc aattctaatg
     5401 ccagttgttc aagtgtagtt ccaggtccaa taaaaatagt atcgccatct ttgattaaag
     5461 agcaggcttt tttagcaatg tcaatttttt cttttgtatt tagacttttc ttctcgatat
     5521 gtgtcttttc aagtggcttt ttagaggaaa gcaattgtgc accaccatga gtacgtgtta
     5581 aaagtccatc agcttctaga gcatcaaggt ctctacgggc tgtcatatcg gaaatatcta
     5641 gttcatctat tatttctttt atggttatgg tcccatcaat ctttaacata tctaaaattt
     5701 tttctaatcg tcgttttttg ttcatatgaa gacccttctt tcataaagta atttttttcc
     5761 aaagataatt ctcttttaat tgtatcataa aagacaatat tttcaaggta aaacaaacaa
     5821 tttcaaacaa aaacaaatgt tagatgatga aataagaaca gaggattgac gtatattagc
     5881 ttaggtcaga ttttgtataa gacgaaaata aagtaggacc tcttaatcag taagttatag
     5941 aaagtaaaag acttttgtaa tacttgaata gatatttcac gtccattttg tgatggatta
     6001 aatgaacaaa aatgaacaat aatttaacgg tgttatctat tttttaaaaa aacaaataaa
     6061 aaaaacaaaa aataaacaaa aaaagtgttg ctttttgttt gaatgcttga tattatataa
     6121 acaaagaaat gatgaaaacg ttttttcgaa cattttgcaa aatgttttct acttctacgt
     6181 agcatttctt tctaaaattt aggaggtggt caaaatggct attgttgttg gtgcagatct
     6241 caaaggtacg agattaaaag atgttgtgaa aaattttctt gtagaagaag gttttgaggt
     6301 aattgatgtg actaaggacg gacaagattt tgttgatgtt accttggcag ttgcctctga
     6361 agtaaataaa gatgagcaaa acttaggtat tgtaattgat gcttatggag ctggtccatt
     6421 catggtagca actaaaatta aaggtatggt tgcagcagaa gtttcagatg aacgctcagc
     6481 ttatatgaca cgtggacata acaattcacg aatgattacg gttggcgctg aaatcgtcgg
     6541 tgatgagctt gctaaaaaca tcgctaaagg tttcgtcaat ggtaaatatg atggcggacg
     6601 tcatcaaatc cgagtagata tgcttaacaa aatgtgttaa gaaaaggagt taaggaatga
     6661 gaatcgcaat tggatgtgac cacattgtca cagatgaaaa aatggcagta tcagaattct
     6721 tgaaatcaaa aggacatgag gtccttgatt tcgggacata tgaccacgca cgtactcact
     6781 accctatcta tggtaaaaaa gtcggtgaag cagtagtaag tggtcaagca gacttgggag
     6841 tatgtatctg tggtacaggt gtcggcatta acaatgcagt caataaagtt ccaggtgttc
     6901 gctcagcgtt ggttcgtgat atgacatcag ctatatatgc aaaagaagag ttaaatgcga
     6961 atgttatcgg tttcggtgga aagattactg gtggtcttct tatgaatgac atcattgaag
     7021 ctttcattga agctgagtac aaaccaacag aagaaaataa aaaattgatt gcaaaaatcg
     7081 aacacgttga aacacataat gcacatcaag cagatgaaga tttctttaca gaattccttg
     7141 aaagatggga ccgtggtgac taccacgatt aaggtataac aaatgattct gacagtcaca
     7201 ctaaatcctt cagtagacat ctcttatcct ctagaaacat tgaaaattga tacagtgaat
     7261 agggtgaaag atgttagtaa gacagctggt ggaaaaggct taaatgttac tagagtcttg
     7321 tatgaatctg gtgataaagt tactgctaca ggtttcttag gtggtaaaat tggtgaattt
     7381 attgaaagcg aattagaaca atctcctgtt agtccagctt tttataaaat ctcaggaaac
     7441 actagaaact gtattgctat cttacacgag ggtaaccaaa cagaaatttt ggaacaagga
     7501 ccaacaattt ctcatgaaga agcagaaggc tttcttgacc actatagcaa tcttattaaa
     7561 caatcagaag ttgtgacaat ttcaggaagt ttaccttcag gacttcccaa cgattattat
     7621 gaaaaactta tccaacttgc ttcggatgaa ggagtagcag tcgttttgga ttgttcaggt
     7681 gcacctcttg aaacagttct aaaatcgtcg gctaaaccta ctgccatcaa acctaataat
     7741 gaggaacttt cacagttgct tggaaaagaa gtaacaaaag atattgaaga actcaaagac
     7801 gttcttaaag agtcactttt tagtggtatc gaatggatag tggtctcatt aggtcgaaac
     7861 ggtgcctttg caaaacatgg tgatgttttc tataaggtag atattcctga tatcccagtt
     7921 gttaatccag tcggatcagg ggactcaacg gtagctggta ttgcatcagc tttaaatagt
     7981 aaaaaaagtg acgctgactt attaaaacat gcgatgacat tgggtatgtt aaatgctcaa
     8041 gaaacaatga cagggcatgt gaatatgact aattacgaaa cactaaactc acaaattgga
     8101 gtaaaagagg tataaaaatg gtacttacag aacagaaacg caaatctttg gaaaaaattt
     8161 cagataaaaa cggtgttatc tcagctttgg catttgacca acgtggtgct ttgaaacgtt
     8221 tgatggcaca gtatcaagat acagaaccaa ctgtagctca aatggaagaa ctaaaggttt
     8281 tggttgctga tgaacttaca aaatacgctt cttcaatgtt gcttgaccct gaatatggac
     8341 ttccagcaac aaaagcattg gataaagaag caggtcttct tcttgcctat gaaaaaacag
     8401 gttatgatac atcaagtaca aaacgtttgc ctgactgctt ggatgtttgg tctgcaaaac
     8461 gtatcaaaga gcaaggcgct gatgccgtta agttcttgct ttactatgat gtagacagct
     8521 cagatgaact taaccaacaa aaacaagctt atattgaacg tgttggttct gaatgtgtcg
     8581 ctgaagatat tcctttcttc ttggaaatcc ttgcttatga tgaagaaatc tcagatgcag
     8641 gttctgttga atatgcaaaa gtgaaacctc gcaaagtcat tgaagcgatg aaagttttct
     8701 ctgatccacg ctttaacatt gatgttctta aagtagaagt accagttaac gttaaatatg
     8761 tcgaaggttt tgctgatggt gaagtagttt acagtaaagc tgaagctgct gacttcttta
     8821 aagcacaaga agaagcgact aaccttccat acatctacct aagtgcaggt gtatctgcta
     8881 aattgttcca agaaacattg caatttgctc acgactcagg tgccaagttt aatggtgtgc
     8941 tttgtggacg tgctacttgg gcaggatctg tagaacctta catcaaagaa ggtgaaaaag
     9001 cagcacgtga atggttgcgt actactggat tcgaaaatat tgatgaactt aacaaagttc
     9061 ttgttaaaac agctagtcca tggactgata aagtatagta aaaacataaa acggaggata
     9121 ttgttatgaa cagagaagag atgactctct tagggtttga aattgttgct tatgctggag
     9181 atgctcgctc taaactttta gaagcgctta aagcggctga aaatggtgat ttcgctaagg
     9241 cagatagtct tgtagtagaa gcaggaagct gtattgcaga ggctcacaat tctcagacag
     9301 ctatgttggc tcgagaagct tctggggagg aacttccata cagcgttacc atgatgcatg
     9361 gtcaggacca cttaatgact actgtcttat taaaagatgt gattcatcac ctcatcgaac
     9421 tttataaaag aggagcaaaa taatgcataa actcattgaa cttattgaga aagggaaacc
     9481 gttctttgag aaaatctctc gaaacatcta tcttcgtgct attcgtgatg gatttatcgc
     9541 tggtatgcca gtcatccttt tctcatctat ctttatcctt attgcctatg tcccaaatgc
     9601 ttggggattc cactggtcaa aagacattga gaccttcttg atgactcctt atagctattc
     9661 gatgggtatc ctagcattct ttgttggtgg tactaccgct aaagctttga cggactcaat
     9721 gaaccgtgac ctacctgcga ccaatcagat taacttctta tctacgatgc tagcgtctat
     9781 ggtagggttc ctcttgatgg cagctgagcc tgcaaaagaa ggaggcttct tgacagcctt
     9841 cacgggaaca aaaggtcttt tgacagcatt tattgcagcc tttgtgacag ttaatgttta
     9901 caaggtctgt gtgaaaaata atgttactat tcgtatgcct gaagaggttc caccaaatat
     9961 ctctcaagta tttaaagact tgattccatt cactgtagcg gttgttctcc tttatggatt
    10021 tgaactcatt gttaagggaa ctcttggtgt aaccgttgca gaatcaatcg gtacccttct
    10081 tgctcctctc ttctcagctg cagatggtta ccttggaatt acacttatct ttggtgctta
    10141 tgccttcttc tggtttgttg gtatccacgg tccttcaatt gtcgaaccag caatcgctgc
    10201 tatcacttat gccaatatcg atgccaactt gcatcttatc caagctggac aacatgcaga
    10261 caaagttatc acttctggta ctcaaatgtt tattgttacc atgggtggaa caggagctac
    10321 attgattgtt ccattcttgt tcatgtggat ttgtaaatca gaacgtaacc gtgccatcgg
    10381 acgtgcctca gttgttccaa cattctttgg ggttaatgag ccaatcttat ttggtgcacc
    10441 aatcgtattg aatccgattt tctttgtacc gttcattttc gctccgattg tcaacgtttg
    10501 gatctttaaa ttctttgttg acacattgaa catgaactca ttctctgcca accttccatg
    10561 ggttactcct ggtccgttgg gaattgtact cggtactaac ttccaagttc tatcatttat
    10621 cttagccgga ctcttggtag ttgttgatac tatcatttat tacccatttg ttaaggtata
    10681 cgatgaacaa attcttgaag aagaacgttc tggtaaaaca aacgatgctc ttaaagaaaa
    10741 agtagctgca aacttcaata ctgctaaagc agatgccgtt cttggaaaag caggcgttgc
    10801 taaagaagat gtagcagcaa acaataatat cacaaaagaa actaatgtcc ttgttctttg
    10861 tgcaggtgga ggtacaagtg gcttgcttgc caatgccttg aacaaagcag cagcagaata
    10921 caatgttcca gtcaaagcgg cagcgggtag ttacggtgct caccgtgaaa tgttgccaga
    10981 gtttgacttg gttatcttgg ctccacaggt cgcttcaaac ttcgatgata tgaaggctga
    11041 aacggataaa ttgggcatta aacttgcaaa aactgaagga gctcaatata tcaaattgac
    11101 acgtgatgga caaggtgctc ttgcatttgt tcaacaacag tttgattaat aaatttgtaa
    11161 aagttatttg agacgagatt attctttcaa ttccttataa tattgttaac catatcattg
    11221 catttcattt ccttggtttc cggaactcag aatatcgtct caaatgcttt ttttgaaagg
    11281 atttatagtt atgactaaaa cacttcctaa agattttatt tttggtggtg ctacagctgc
    11341 ttatcaagca gaaggtgcga ctcacacgga tggtaaaggc ccagttgcat gggataagta
    11401 tcttgaagac aattattggt acacagcaga gcctgcaagt gatttttacc ataaatatcc
    11461 tgttgattta gaattagctg aaaaatatgg cgttaatggt attcgtattt caattgcctg
    11521 gtctcgtatt ttcccaactg gctatggcga agtaaatgaa aaaggtgttg aattctacca
    11581 taatcttttt gccgagtgcc acaaacgtca tgttgagcca ttcgtgactt tgcaccactt
    11641 tgacacacca gaagttcttc actcagatgg agatttctta aaccgcgaaa atatagaaca
    11701 ctttgtagat tatgccgctt tctgttttga agagttccca gaagtaaact actggacaac
    11761 tttcaatgaa attggcccaa ttggagatgg tcaatacttg gttggtaaat tccctccagg
    11821 cattcaatat gatcttgcca aagtcttcca atcacaccac aatatgatgg tttctcatgc
    11881 acgtgctgtg aaattgtata aagataaagg ttataaaggc gaaattggtg tagtacatgc
    11941 gttgccaacc aaataccctt atgatccaga aaatccagca gatgttcgtg ccgctgagtt
    12001 agaagatatc atccataaca aatttatctt ggatgcaacc taccttggac actattcaga
    12061 taaaacgatg gaaggggtca accacatcct agctgaaaat ggtggagaac ttgatcttcg
    12121 tgatgaagac ttccaagcac ttgaagcagc taaagatttg aacgatttcc ttggtatcaa
    12181 ctactacatg agtgattgga tgcaagcctt tgatggtgag actgaaatca ttcacaatgg
    12241 taagggtgaa aaaggaagtt ctaagtacca gattaaaggt gttggtcgcc gaatcgcacc
    12301 agattatgtg cctagaacgg attgggactg gattatttat cctgaaggct tgtatgacca
    12361 aatcatgcga gtgaaaaatg attatccaaa ctacaagaag atttacatca ctgaaaacgg
    12421 tcttggatac aaagatgagt ttgtagataa taccgtttat gatgatggtc gtattgatta
    12481 cgtgaagaaa cacttggaag ttttatcaga cgcaattgcg gatggtgcaa atgttaaagg
    12541 ttacttcatc tggtcactta tggatgtctt ctcatggtca aacggatacg aaaaacgtta
    12601 tggactattc tatgtagact ttgacacgca agaacgctat cctaagaaat cagctcattg
    12661 gtataagaaa gtagcagaaa ctcaagtgat agagtaatat tacttgaaaa tgtaaaaaat
    12721 tgttatccga tactttagaa aaagtacaac acttatttag accaggcttt gaaaaggtat
    12781 atattgttag tttcgaagat tgtcctatga ttcctgagct tgaagctacc ccgttgctaa
    12841 aatgtggtaa atggtatgtt tcaactggaa aagagtggat ttgccattct gatttagagt
    12901 tatcagcatt tgaatgggaa tttttacaat cactcgatgt agaaatacgt gagactattc
    12961 acttcgaggt aaattatttg cctttccaat aataaaaaga aaggaattag ttgtgactat
    13021 cgaattaaaa aacgagtatc ttactgttca gtttaaaaca cttggtggtc aactgacttc
    13081 gattaaagac aaggatggga tagagtatct ctggcaagct gatccaaatt attggaatgg
    13141 acaggcgcct attctatttc ctatctgtgg tagtttacga aatgattggg ctatttatag
    13201 acctcaagaa agaccctttt ttacaggtct tattcgtaga catggttttg ttcgaaaaga
    13261 agaatttatt ctggaagaag tcaatgataa tagcgtgacc tttagtatta aaccaaatgc
    13321 tgagatgctt gataattacc tatatcagtt tgaactgaga gttgtttata ccttgaatgg
    13381 taaatcaatt agaaccgaat ttcaggtaac gaacttggaa atggaaaaaa caatgcctta
    13441 ctttattgga gcccatccag cattcaatag tcctttagta gaaggtgaaa actatgagga
    13501 ttattatatt gaatttagtg aggttgagtc ttgctcgata ccaaagagtt ttcctgagac
    13561 aggattatta gatttgcaag atcggacacc ttttttggaa aatcaaaaaa ttctcgattt
    13621 ggactactca cttttttccc atgatgccat tacacttgac cagctcaagt ctcgtagcgt
    13681 aacgcttcgg tcaaggaagt ctggtaaagg tcttcgagta gattttgatg attttccgaa
    13741 cttgattctg tggtctacaa ctaataaaag tccattcatt gctttggaac cttggagtgg
    13801 tctctccact tcacttgaag aaggtaattt cttagaagat aaaagacagg taacgaaaat
    13861 ttcacctcaa gaaacttcta gaaaaatcta tgatattact atccttaatt aaaatttgtt
    13921 attatttgag tcttcaataa gatttgaata ctcttacctt gtgatgatag attggtcttc
    13981 tccgtgccag tcttacatcg caaggttttt aagttttttg tgggtctatg tcaactgtag
    14041 tgggtgacga aaagctaata tctaaagaca gtctggtagt aaaaaataag attatttact
    14101 acttcttgat ttaaaataat agtggagcca ttaatttgtg gcaaatttaa tttaatattt
    14161 tgcctcaaat tagccacact ttttatagaa ctaagaggaa aaatgaaatt ttcttcctat
    14221 taatatagtt gtttgaaatc ttaaatatag cgttttggaa tggcaacagt ttttttgaca
    14281 aattttataa ggtgcagaac ttctttccgt atgctattcc gattgtcctt gacaatgagc
    14341 ctcctcggat gtgacgatct tcggcaggag ctaa
//
LOCUS       DAHHPA010000072        13173 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.073, whole genome shotgun sequence.
ACCESSION   DAHHPA010000072 DAHHPA010000000
VERSION     DAHHPA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.073"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..786
                     /locus_tag="NBH55_002023"
     CDS             <1..786
                     /locus_tag="NBH55_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510000.1"
                     /translation="LIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVK
                     SLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHA
                     NKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSN
                     KLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVI
                     TYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(931..1671)
                     /gene="truA"
                     /locus_tag="NBH55_002024"
     CDS             complement(931..1671)
                     /gene="truA"
                     /locus_tag="NBH55_002024"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCD1510001.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1862..2497)
                     /locus_tag="NBH55_002025"
     CDS             complement(1862..2497)
                     /locus_tag="NBH55_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCD1510002.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2644..3885)
                     /gene="sstT"
                     /locus_tag="NBH55_002026"
     CDS             complement(2644..3885)
                     /gene="sstT"
                     /locus_tag="NBH55_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCD1510003.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4083..5081
                     /locus_tag="NBH55_002027"
     CDS             4083..5081
                     /locus_tag="NBH55_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510004.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5094..7112
                     /locus_tag="NBH55_002028"
     CDS             5094..7112
                     /locus_tag="NBH55_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCD1510005.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7172..7888)
                     /locus_tag="NBH55_002029"
     CDS             complement(7172..7888)
                     /locus_tag="NBH55_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCD1510006.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8115..8657)
                     /locus_tag="NBH55_002030"
     CDS             complement(8115..8657)
                     /locus_tag="NBH55_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1510007.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8662..8889)
                     /locus_tag="NBH55_002031"
     CDS             complement(8662..8889)
                     /locus_tag="NBH55_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD1510008.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8968..10848)
                     /locus_tag="NBH55_002032"
     CDS             complement(8968..10848)
                     /locus_tag="NBH55_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCD1510009.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11037..11651)
                     /locus_tag="NBH55_002033"
     CDS             complement(11037..11651)
                     /locus_tag="NBH55_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1510010.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11793..12353
                     /locus_tag="NBH55_002034"
     CDS             11793..12353
                     /locus_tag="NBH55_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1510011.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(12658..12972)
                     /locus_tag="NBH55_002035"
                     /pseudo
     CDS             complement(12658..12972)
                     /locus_tag="NBH55_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         4127 a         2585 c         2076 g         4385 t
ORIGIN      
        1 ttgattggtg cttttatttt tataaaaaaa ttaactgctg atgatttatt tattttcttt
       61 cttggtgtat ttgttctttt ttgttcaacc aatttatttc cttggcaact ttttaaccat
      121 acaccagtta agagtctaca atttgtctgg cggttaaatg gtttttcttc tttatttata
      181 gcctatacga tgagtatagt gatctattat attttttcta ctaaaaataa ttcatggaaa
      241 ataatggtct ttacgtttct tgcactaata ttgcatctct caggagtaag caattctatc
      301 catgctaaca aagattcatt aacacttata gcacctgaag acgctgttgc tatagctgaa
      361 aattacaatc atactgatta tgcaaacaaa gagtcaatct atcatcctga tatgattaat
      421 aatgatcaat atttactagg taaccaggaa ataaatccaa caactcattt tatgagtaac
      481 aaacttacaa ttgaattaga taattcgaat aataaaaata ctgtcttgac gacacctatt
      541 tatcgttata aagggcaagt ggcttctatt aatggcaaac tagtacaaac aaaattatct
      601 aagtttggca caactgaatt aactattcct ccaggtataa ataaagtcgt tattacttat
      661 cagtacacaa aactagcaat tgcttcaaga tatttatcta tcgttacttt gattttattt
      721 ttactttatc gatttacttt ttctaaacaa aaacaaccga gaagagaagt acaacattct
      781 cactagattt ttatttaaga aagacttttt agctgtacac ataaacaggc taaaaagtct
      841 ttttttatat ttctttaatt tgcttcatac attatttaac atcgatattt atactgtgaa
      901 acatgcgttt catccattat caaagtattt ttatcgataa taaacttcat ccaaaaacag
      961 accttgtgca gggacggttt ctcctgcatg ttgccgcact ttgctttcaa atatttcatc
     1021 gattgtttct atcggcatgg cacctgtgcc gatttctagt aatgtcccca tgataatccg
     1081 caccatttta tataagaatc cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg
     1141 ttcgatagat aattcatcaa tcgtacgcac tgtggatttt tttgattttt tcaatgcaga
     1201 aaaaccaatg aaatcatgtg tacccactaa tttttcgcaa gctgcattca gcttgtccat
     1261 atccaactct tgagggaaat aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt
     1321 gttccaaaca taatagctgt attttttgcc caccgcatta tatcttgaat ggaagcgctc
     1381 aggcatttct tcgattcttt tgatgacaat atcccgcgga aggtaacgat tcataaagtc
     1441 gatcatttct gccagtccaa tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc
     1501 atgggtgcca gcatctgttc ttcctgatcc aataatttct atctcttcat ttgtcatctg
     1561 atgcaatact ttttcgattt ttccttgaat ggttttatcg tcatctccaa gtctttgcca
     1621 tcctgaatat ctttttccgt catattcgat tgtcaacttg atgtttctca tattttagtg
     1681 gctcctctgt cttccatttg gtcttctctc taccttttta agattgtagc acaaaatata
     1741 gctccttgct tctctaagac agaagctata caagaaaact gtggaaatat atatttcaga
     1801 aaaacaaaaa acagccaccg gatgaagtat ccgctgactg ttcaatttga cggctcaagt
     1861 tttactgttt gagatgcaag atactcaacc aggcaatggg cacataatta aagaaaaaac
     1921 tgattctttg gcaaaaacct attttattca gtaatggcca agaatttata gaaggaattc
     1981 ttgccagtcc ataaatcgcc gcaaaaagca aactgatcac agacagtcca aggtataagt
     2041 ggcttacgtc catttgccca ttttttcgat agatcaagaa aagaagaact ggaaacaata
     2101 aaaaaccact gtaacctaaa cctgatccga tattatggac ccatgtcgag acattccacg
     2161 aagattcatg ggtattgata ctgaacaaac ctgtaaagat acaatcacct ataccataga
     2221 acgcgatggc tgctgtaact agggtagcta acacacgaga tgtactaaca aatgcagcat
     2281 acaatgcagg caatgacagg atgaaaaaga tccctgaaac cacagaccaa ataagaaacg
     2341 cttttcttac aggactggaa acctctccaa atacactgat cacgctcttc atttgatcta
     2401 attcaggata atacaatcct aatacgtacg gagtcaaaaa atcactcagc acgcctaata
     2461 ataaaaagaa aaaaccaaat ttacgtaaaa aatccatgat acgcctccaa taacaattac
     2521 ctatgctttt aattgttatt ataaacgact ttatcttgat ttaacagaaa cctctattta
     2581 cacgaacatc tatataactc attaaaaact cgaatcattt taaaaatact aaatatctaa
     2641 cgttcaacgt tcacttaatc catctaaaca atctttcaat ttgatttttt ccccacttct
     2701 tttacgcgcg cctagctctc ctattgcagt aaacaacaga tcacttgacg aatttaaggc
     2761 agtttccatt gaatcctgga taacaccgat aataaatcca ataccgacga tttgcatcgc
     2821 gacatcatta gagataccaa acaaactaca tgctaatgga atcaataaca gtgaaccgcc
     2881 cgcaacacct gaagctccac aagcagctaa agcagacaaa atacataaaa ggactttcaa
     2941 aacaaaaggt gcttcaatcc ctaaagaatg tacagcagtc aacgtcatca aggaaatggt
     3001 aatcgcggca ccgcccatat tgatcgtagc acctaacggg atcgatatgg aataagactc
     3061 ttttgtcaat ttcaatcttt cagccaacat catgtttatc gggatatttg cggcagaact
     3121 tcgggtaaag aaagcgggaa gtgcgctttc tttcaaacaa aagaaaatca atggataggg
     3181 attttcttta ataaacaaaa acgtgaggaa tgggtacaca actaatgcga caaacagcat
     3241 cgtaccgatc aaaacaagta aaagctgcag atactctgcc aatccagcga ttccagtcgt
     3301 agcaattgat tgataaacca atcctaaaat ccctatcgga gcaaatgcaa taatcacttg
     3361 tacgacttgt gacaacgcta tagatatctg atccaccaac tcttttgtcg attctgaacg
     3421 tgctcgaaaa gctagtccca acaatacagc ccagaataag acgccaaggt aattcgcatc
     3481 aataagagat tgaacaggat tttgtgtgac attcgtcaac atagtagata aaacactttc
     3541 caaactttgc ggtgcttttt cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat
     3601 tgaacttaca ataactgcta caagtgcaga aagcaatgtt cccactaaat ataagatcaa
     3661 aataggcttc acgaatgtct tattcccaat tttatgcttg gcaattgacg ccatcgtcaa
     3721 gaaaaaaacc agaataggtg cgattgcctt caagcaactg acaaacaacg ttcccaatat
     3781 tgaaataaat gtccatgtgg ggaaaaagat accaaacaat attccgacta aaataccaac
     3841 aacaatacgt tgaactaacg acatatccaa aaacttctct aacaaatgac caccctttca
     3901 caattttaac aatcattgta tcattttatg caaagaaact aaaggtataa ttttctaaaa
     3961 attctaacaa tacaaacaac catttcttgc taattttgta ctttttctgt ctacacgttg
     4021 ttttttgaga tattctaagt taggatgtta ttaatgggca acttatcagg aaacgttttc
     4081 ttatgttttt gtccaatttt tttgtgaggt gtttattctt gaaaaaaata aagaaggaga
     4141 gttatggctt cctgacaatc tttgtcgctt gtataatgat ggttttacag cgtatattga
     4201 tttcagcagt cactattcct acatatgcaa caaaccctgg tcgattctac atctatacat
     4261 ttttacaaac ttccgttgtt attggtgcaa atttgatccc actttacatg ggttaccaat
     4321 atcaaaaaat cgcccgttta aaagttttac attatttaag tcgttttgcc tttatattcc
     4381 taatagccac actagtttgc aatatctttt tttacgtaca agctggcagc ttgaatattc
     4441 gtgattattg gcttatccta acacctatca gtcagaatta ctttacttat gctgtttctt
     4501 gtactttgct tttttgtagt attccttata cagttggatt tttaaataaa ttatcgaaaa
     4561 caacaattaa atatctacta ttatttttaa caattggatt agttggctgc tctactctat
     4621 ttaataaaga cccgtttgca ttacagaatg gacagagtat tttatggatt ttttatttat
     4681 ttttaatggg ttatggtttg aaagaatgga actggaaaga aaaggtccgc tataagcttc
     4741 ctcaagtatt tatcagcttg attgttttat tcggtttgat tattttgatg acacaaattt
     4801 cactcatcat cagaggaaat acagatacag cgctgaggtt ttctaaacca tattctctct
     4861 tcgcaatgta ttatactttc tcttcgtttt tatttttaga aatgctatct gataaattag
     4921 gtataaaagg aagaggaaat tttcttgctg tatttttgat tgtcactcaa gttatatgta
     4981 atttcccttt agtttcttat cgcgtatcta catttatgaa acgtgaattt ccaaactcag
     5041 ggaaggcatg gctgataaat atcgggcagt tttttatttg atatttatta gcagtgtccc
     5101 tcctagttat ctttttattt tatatacaaa aaatccgttt cattcatcgt ttcatcgaat
     5161 acttcgcttt tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc
     5221 tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc
     5281 agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa
     5341 tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct
     5401 tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta
     5461 acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc
     5521 cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct
     5581 tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt attgtacaac
     5641 gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg
     5701 ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt
     5761 tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa
     5821 aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac
     5881 ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag
     5941 atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa
     6001 gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc
     6061 cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg
     6121 gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt
     6181 ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata
     6241 ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca
     6301 gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata
     6361 aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca
     6421 aagaaacctt gaaagcttta caaagcaata cggaaacgtc acaattcatc caattatcca
     6481 ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag
     6541 gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact
     6601 ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta
     6661 cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat
     6721 atggattaga acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac
     6781 aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg
     6841 agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc
     6901 tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg
     6961 tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag
     7021 aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg
     7081 caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata
     7141 aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat
     7201 taaagccatt tttcaattca ttattcgctg tctcgttgaa gtaaagataa ttatcgggat
     7261 tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat
     7321 agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat
     7381 agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag
     7441 ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac
     7501 tcatttgatt cgtataactt ttgtattgtt gtcccattgt ttggccccat ttggtcataa
     7561 atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc
     7621 tttctgaaga ggaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt
     7681 cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttctgta cttgctgcag
     7741 ttgacttgct ctctgtgcta gttgttgtag tagttgcaac tgttgctttc gctgtttctt
     7801 ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac
     7861 caattgttac taaaatcctt tttttcaatt aaaaaactcc tttcgattac tcccacttct
     7921 ttattctatc aaataagcaa tgtgatttta ttgatttttc gtattttctc atcaatcttt
     7981 caaatcttct aaaaactgag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc
     8041 ttcttttatt ttaatcttcg tgattttcca atttacctca cgctttatat ttgtatttga
     8101 agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt
     8161 ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc
     8221 aattgttgca agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag
     8281 acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat
     8341 ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga
     8401 acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga
     8461 tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat
     8521 tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ctaatacatg ccccaccatg
     8581 gcgccagcag ttggtttatg atggtcgata tctgcttgtt ttgtttcttg aacaaatttt
     8641 tcttccacta tattcattga tttacgcctc ttttactttt gctgattgga cttcataacc
     8701 taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc
     8761 tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac
     8821 cgcatgttcg attttttgca tacagcttgg acaagctagt gtatctaatt gcataataat
     8881 ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc
     8941 cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa
     9001 atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat
     9061 cccactagcc atgtaaataa acccggcaaa taagccgttg agtaaaaata agacgacacc
     9121 aatggcaatc actacatttt cacgggtatt taaaacagtt ttcttagcca agcggtaggc
     9181 atgaaccaac gattcaaaat tagattgcat caagacaacg tcactcgttt cgatagcaac
     9241 atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt
     9301 gattccatct ccaataaaag caacgcgcaa tccttgttct tggtattttt tcacaaattg
     9361 aaccttttcg tctggtaata attcagcatg gacttcatca atgcctaaca tatcagctac
     9421 ataatcagca gtcatctgat tatcacctgt cagcatcact aactgttttg ctcctgcttg
     9481 tttcaactta gctaattgtt cagcgacttc tggtcgaatc acatctgaca cacctatgat
     9541 ttgagtgatt tttttgtcga ttgctacaat aatgaccgag ctgcctgttt gttgtaattg
     9601 atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt
     9661 gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgattgt
     9721 ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat
     9781 cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct
     9841 gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa
     9901 tgtaccagtt ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc
     9961 gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg
    10021 cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgattg ctaaacgaaa
    10081 atcacgacta atcagaccaa caaccaacgc aataagtaaa acggttggag tataatactg
    10141 agaaaatcga tcaatgaatt tttccgcatg ggatttggta tcttgtgctt cttctactaa
    10201 ttcgataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta
    10261 gccatctgct aaaatcgttc cagagaagac ttcatctcca ctgattttga cgacttgtcg
    10321 tgattcacca gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc
    10381 cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat
    10441 ttccacttct tcagtcgttc catcttcttg gacaacacta gcagtagatg gggccatctc
    10501 ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc
    10561 aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc
    10621 aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttca ctgcctgata
    10681 cgcatggatc ataataggta tgaccgcaat aatagaagct atggctagag caatgttata
    10741 gaacaagaca aaaccagtaa attttccgat aaaaccaaaa ataatcaaca cagctgataa
    10801 ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct
    10861 tttcctttca ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac
    10921 tatcaagttt agtcaaaaag gcggttttct tttggtagaa tacactacaa aaaatagtta
    10981 gcaagaagac tcactaaaag tgatcttgcc aactattgtc gtttctatcg aaattttcat
    11041 tgaatgattt tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc
    11101 ttgctaattt tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag
    11161 ccactatctg taaaaaggaa tatttctttt cctttctctc ttttttcaaa ttctgttagc
    11221 aacttggtac cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa aatgatctct
    11281 ccatctggcg aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa
    11341 gctcctttac tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt
    11401 tgctgtccaa aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct
    11461 gccaactcgt cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac
    11521 aaaaaatatc ttccgtaaag attcagcaga agttcatttt ccatatatct ctcaaagttc
    11581 attccttcaa cagcaaaata gatagctttt ttgtaatctt tcttttctaa gggtttaaaa
    11641 gttagtttca tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata
    11701 gacacagtgt ctactatcta taatcaatca tctataagtt tgtcgattaa gcgacagctt
    11761 tgctttgata tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa
    11821 aaacagaaaa agctttggct gaaacactat ccacattatt agtaaataaa aagattcaag
    11881 caatcactat acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac
    11941 attataagga tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa
    12001 atgaaattct ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg
    12061 actatgttta cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt
    12121 cgtttcagaa gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg
    12181 aagacaagaa aacgattatc acagaagaaa tgagattctt tactacgtac cacgtccaag
    12241 gttgtctatc cgttctcaat cggtgggtag aaagcaatta caagtactcc aaaaaagaat
    12301 tattagaact cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa
    12361 catactaagc ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta
    12421 gccaaattgt ttactgaaga ttttttgagt aaaatttcta taattcctac ttattgaaaa
    12481 gtagtttgta gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt
    12541 cacctcatca ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg
    12601 taaccaattt atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta
    12661 cctcaattca ttttcaaggg gaatcagctc atcatctaac attttacgcc atgctcgctt
    12721 catttcttgt gggtatttaa atggtttcgc ttctggatgt ttggctttat atccttcgta
    12781 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
    12841 cagattagaa gcatgtaatt ttttctcaga aacaaaccac tcattggaaa agacatgaat
    12901 caaatcatca cttacttgtg taaaatagcg acgtttaatg acaaagatat cttcattgct
    12961 atctagtgta ccttcatttc tttgtgctta aattcaaaga taattttctc aataaagatt
    13021 tttgattcca gtaagattcc tttactcctt ttacaacata gcgttgtcct ttgttttgaa
    13081 acatcttctt cccctctttg ccattaaaaa tagcccaatt ctctataaag aagattaagc
    13141 ttcattatta taatataaag tttaaatttg gtt
//
LOCUS       DAHHPA010000073        13127 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.074, whole genome shotgun sequence.
ACCESSION   DAHHPA010000073 DAHHPA010000000
VERSION     DAHHPA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.074"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            162..1103
                     /locus_tag="NBH55_002036"
     CDS             162..1103
                     /locus_tag="NBH55_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCD1510012.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1172..1903
                     /locus_tag="NBH55_002037"
     CDS             1172..1903
                     /locus_tag="NBH55_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCD1510013.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2047..4227
                     /locus_tag="NBH55_002038"
     CDS             2047..4227
                     /locus_tag="NBH55_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCD1510014.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4247..4729
                     /locus_tag="NBH55_002039"
     CDS             4247..4729
                     /locus_tag="NBH55_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCD1510015.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4745..5506
                     /locus_tag="NBH55_002040"
     CDS             4745..5506
                     /locus_tag="NBH55_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCD1510016.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5637..6971
                     /locus_tag="NBH55_002041"
     CDS             5637..6971
                     /locus_tag="NBH55_002041"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCD1510017.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7278..7763
                     /locus_tag="NBH55_002042"
     CDS             7278..7763
                     /locus_tag="NBH55_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCD1510018.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7756..8439
                     /locus_tag="NBH55_002043"
     CDS             7756..8439
                     /locus_tag="NBH55_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCD1510019.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8565..11210
                     /gene="polA"
                     /locus_tag="NBH55_002044"
     CDS             8565..11210
                     /gene="polA"
                     /locus_tag="NBH55_002044"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCD1510020.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11255..12091
                     /gene="mutM"
                     /locus_tag="NBH55_002045"
     CDS             11255..12091
                     /gene="mutM"
                     /locus_tag="NBH55_002045"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCD1510021.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12055..12684
                     /locus_tag="NBH55_002046"
     CDS             12055..12684
                     /locus_tag="NBH55_002046"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCD1510022.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12736..13068
                     /locus_tag="NBH55_002047"
     CDS             12736..13068
                     /locus_tag="NBH55_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD1510023.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT         4429 a         2321 c         2896 g         3481 t
ORIGIN      
        1 ttttctgtgc gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac
       61 aaaaaacttt ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc
      121 tttatactgt ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc
      181 tttattacaa ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct
      241 tttgcaaatc tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta
      301 ctgtgtctgg aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc
      361 gatttgctga tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt
      421 ttgtaagata ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc
      481 aagtgacagg tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga
      541 cagtggtgat tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg
      601 tacgcccaga tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag
      661 aatttatgga aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt
      721 ctggttggct tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta
      781 cttatggtaa ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg
      841 atgaacggat cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt
      901 ttgacggaac accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga
      961 ttcttgcttc cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga
     1021 aacatccgcg caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc
     1081 ggtttacagc tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg
     1141 cgctatacta gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga
     1201 tgtccatggc aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca
     1261 tcagcttgtt ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt
     1321 attaggaaag caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga
     1381 atattttttg caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg
     1441 tggaaaagaa acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac
     1501 tgaaatctcg atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg
     1561 ccccttgtat tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa
     1621 gaaagactgg cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga
     1681 agggaaaaac aatacaggta agacaattgt atttggacat acgattacac caatgctcca
     1741 tggagatatg cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg
     1801 ggctgtattc ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga
     1861 tattgaatat cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa
     1921 ggagctgtga ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta
     1981 gtttaaccca ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc
     2041 attttaatga cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca
     2101 gactatcgtc ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt
     2161 ccttttatcg cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt
     2221 gaaatcgaag agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt
     2281 cggctaatcg atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc
     2341 gtgaaaatgc aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa
     2401 gcaacgattg ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca
     2461 gatggagagg tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct
     2521 gccgaagaag cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca
     2581 aaatttcgga cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc
     2641 aaggatgagc aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc
     2701 gtccataaaa tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt
     2761 ctgaaagtat tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata
     2821 gtcaaaaatc ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat
     2881 aaacgcttta ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca
     2941 gatgagcaag caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca
     3001 ctaaaaggta aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca
     3061 gtcgtagatg cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca
     3121 gcgcaagcaa aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa
     3181 gtagatatgg tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct
     3241 gagcagttga aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct
     3301 tctgtttact ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa
     3361 agaagtgctg tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc
     3421 gatcctaaag ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct
     3481 gaacaattgg attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca
     3541 gcaagcccgc agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt
     3601 gtcatttatc gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca
     3661 cgcttaggac caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa
     3721 aacattttgg acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta
     3781 acttcagtcc aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca
     3841 cgattatccg tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat
     3901 attttagctg ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta
     3961 ttacgtacag atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg
     4021 gtccgtaatg tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt
     4081 gtccatatat cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta
     4141 ggagatatcg taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg
     4201 acgatgcttt ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga
     4261 actgcagcag ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca
     4321 agcaatgttc aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca
     4381 tttagatttt aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat
     4441 caaacatgaa ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga
     4501 tatggatttc aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac
     4561 gaagaaacag ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg
     4621 caagagaaga atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg
     4681 gattgagaca aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag
     4741 gcctttggaa aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt
     4801 agccgcacct gaagagggaa attttgagga actgacggca cctacactta ttttagctgg
     4861 aaactgtctt cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga
     4921 tcaattgttt ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga
     4981 aaagcaagga tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct
     5041 aaaagaagtc tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc
     5101 tggagagaac gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa
     5161 aatcttattg atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt
     5221 atggcaaaat gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca
     5281 cttttctgaa gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata
     5341 tttccatgcg ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg
     5401 tccgaaagga aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta
     5461 caccagaatt aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc
     5521 gaatcctcct ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga
     5581 gcgtgttgaa tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg
     5641 aaaatcaaaa taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg
     5701 ctcttgtttt acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct
     5761 ttacacaaag agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg
     5821 taaaaccagg gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc
     5881 agcgagcaaa agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta
     5941 tccaaaatta tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac
     6001 tgctctctca tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag
     6061 gacatggtga tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt
     6121 tccttgccta ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt
     6181 attatacaag tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa
     6241 aagctatttt tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga
     6301 tttattatta tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa
     6361 cttctggttc tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac
     6421 cagcattcgg taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg
     6481 aaaaactgga tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc
     6541 gattcagtga aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg
     6601 cagagatcaa agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg
     6661 cagttttcca accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag
     6721 cacttgattt agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac
     6781 aaggaaacgt taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca
     6841 aagaaaataa tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg
     6901 ctggagatgt tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa
     6961 atgtactgta atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa
     7021 aaatcttgcc aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc
     7081 tgcggtaata agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt
     7141 attatttgat gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc
     7201 aagggagcag aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa
     7261 actggagggt ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg
     7321 aaatcgagga aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc
     7381 tgtatttggg attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa
     7441 aaacagaata tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg
     7501 caatttcaaa gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta
     7561 ttgaaccggt tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata
     7621 agatgaagga tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt
     7681 tggatgctgt cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa
     7741 gtgaaggtga attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat
     7801 gcaaaagttt atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg
     7861 gctctttggg tatttccttt gcaattagca acttttatca ataattttcc actaggcttt
     7921 gtgggtctgt ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag
     7981 aatccgagct tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta
     8041 gcggttactc ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact
     8101 ttgatgttca tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt
     8161 ggaagagcag gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc
     8221 attttgagaa gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca
     8281 gggattacag cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca
     8341 gcacatagtg gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg
     8401 ttcttggcgt ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt
     8461 tggggctata ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt
     8521 ctttgttaga atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat
     8581 tattactcgt tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc
     8641 ttgaacgttt caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga
     8701 tgtttgaaaa tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag
     8761 gaaaaacgac ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac
     8821 ctggcgaatt caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac
     8881 agtattacga attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag
     8941 tagacaaaga tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag
     9001 ctagtaaaga agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata
     9061 caccagaaca tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga
     9121 agggactagc cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa
     9181 cagctatcaa gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg
     9241 aaatgaagca aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat
     9301 cgaaaaaatt ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga
     9361 agtatgaagg gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc
     9421 agttcctttc taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg
     9481 aagtcgttca tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa
     9541 tgatgggcga caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga
     9601 aaatttatgt cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca
     9661 ctgatccgac tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc
     9721 gctatactgg aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg
     9781 atacgaatga aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata
     9841 tccaatcaga tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg
     9901 aactcttttt tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat
     9961 tgagccaaga actggcagat aaaaaccaag aagatctttt ccggaaaatg gaactgccat
    10021 tgtctatcat tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac
    10081 aagaaatgaa gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag
    10141 aagccggcga agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa
    10201 aaatgggatt acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac
    10261 tggaacagct tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg
    10321 caaaaattca atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag
    10381 tgcatacccg ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta
    10441 atttgcagaa catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc
    10501 ctagagaaaa agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac
    10561 ttgcgcatat ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc
    10621 atgccagcac agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata
    10681 tgcgtaggca ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac
    10741 tttcacaaaa tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg
    10801 aaaaataccc aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag
    10861 gctacgtcga aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt
    10921 acaatatccg ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg
    10981 ctgatatttt gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc
    11041 aagctacgat gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac
    11101 ttgaatcatt agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac
    11161 cactgatcac tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta
    11221 agacagagca atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac
    11281 cgttcgtaaa ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt
    11341 gtggcccaag atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga
    11401 aaccatccaa tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga
    11461 actgatctca cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat
    11521 cgataaacat acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga
    11581 tgtacgaaaa tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg
    11641 aatcatgaaa ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa
    11701 tggactgaaa aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac
    11761 tggtctaggt aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca
    11821 accggcgaac acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt
    11881 tttggatcgt gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg
    11941 agaatcaggc ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat
    12001 acgttgtgga acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc
    12061 gaattgtcaa aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata
    12121 gctaccggta aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat
    12181 ggcgatgtga ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta
    12241 tcagtgtttg gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg
    12301 atgattgttt ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt
    12361 atccgaaaag cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt
    12421 gtagatatcc cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc
    12481 gtttatacta cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgctg
    12541 gaacaagcgc agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg
    12601 gatattttgt tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg
    12661 ctttttgcta atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga
    12721 aagtgaggga tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca
    12781 aaagaaaaaa tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa
    12841 tcagaaacag cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac
    12901 cagcaggcag aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca
    12961 cctgatcgta gtaaaacacc gctcccagaa agtgaccaca tttccgatga gcaaaaaatc
    13021 gaggaacagc gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa
    13081 tcgagatgtg acttgaagca gatgagaagg tgttcatctg cttcaag
//
LOCUS       DAHHPA010000074        12977 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.075, whole genome shotgun sequence.
ACCESSION   DAHHPA010000074 DAHHPA010000000
VERSION     DAHHPA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.075"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(192..1640)
                     /gene="cls"
                     /locus_tag="NBH55_002048"
     CDS             complement(192..1640)
                     /gene="cls"
                     /locus_tag="NBH55_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD1510024.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1792..2685)
                     /locus_tag="NBH55_002049"
     CDS             complement(1792..2685)
                     /locus_tag="NBH55_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1510025.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2669..3355)
                     /gene="ftsE"
                     /locus_tag="NBH55_002050"
     CDS             complement(2669..3355)
                     /gene="ftsE"
                     /locus_tag="NBH55_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCD1510026.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3460..4561)
                     /gene="prfB"
                     /locus_tag="NBH55_002051"
     CDS             complement(join(3460..4488,4490..4561))
                     /gene="prfB"
                     /locus_tag="NBH55_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCD1510027.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4687..7221)
                     /gene="secA"
                     /locus_tag="NBH55_002052"
     CDS             complement(4687..7221)
                     /gene="secA"
                     /locus_tag="NBH55_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCD1510028.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7443..7994)
                     /gene="raiA"
                     /locus_tag="NBH55_002053"
     CDS             complement(7443..7994)
                     /gene="raiA"
                     /locus_tag="NBH55_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCD1510029.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8122..8841)
                     /locus_tag="NBH55_002054"
     CDS             complement(8122..8841)
                     /locus_tag="NBH55_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCD1510030.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYRWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(8838..10151)
                     /locus_tag="NBH55_002055"
     CDS             complement(8838..10151)
                     /locus_tag="NBH55_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCD1510031.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKVCQEIKEMNEEGKKML"
     gene            complement(10232..10552)
                     /locus_tag="NBH55_002056"
     CDS             complement(10232..10552)
                     /locus_tag="NBH55_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1510032.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(10660..10947)
                     /locus_tag="NBH55_002057"
     CDS             complement(10660..10947)
                     /locus_tag="NBH55_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510033.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            11074..11718
                     /locus_tag="NBH55_002058"
     CDS             11074..11718
                     /locus_tag="NBH55_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCD1510034.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHGQLITDHI"
     gene            complement(11777..12484)
                     /locus_tag="NBH55_002059"
     CDS             complement(11777..12484)
                     /locus_tag="NBH55_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1510035.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(12704..12874)
                     /locus_tag="NBH55_002060"
     CDS             complement(12704..12874)
                     /locus_tag="NBH55_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510036.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3589 a         2813 c         2254 g         4321 t
ORIGIN      
        1 ttgaaatgtg tctcttttaa tagcatatat tagcttattt cttgctttcc ccaaaaaaca
       61 agagataccc caaagttaat ttcgctcatg cgagacgctt tctccatttc tgacgtactc
      121 tcagaaatgg ccccgttaga aaaggacacg agatctccat ctgttgatgg ttctcatgtc
      181 ctttttatgt tttaaaggag cggcgaaatc aatcttgcta atccttcttt catcttgatg
      241 attattcctc ggttttcata catttctttt gtgagatgcg tagaaatttt cgagtcttca
      301 aaaaaagctt ttcttacacg tgaagccatt cgttcatcat aaaccagtgt attgacctca
      361 aagtccaagc gaaatgaacg aacatcgata tttgctgagc caactgaaac aatgccacca
      421 tcgataatca tcgtctttgc atgaataaat ccattctcat atgtttcgat ccttgctcca
      481 tactcgatca gctctgcagc gaaagaatac gttgcccagt aaacgagaag atgatctggt
      541 ttgttaggta tttggatatg aacttttacg cctgaaagaa gagccaactt cagtgcttca
      601 tgaatcgatc catccggtat gtaataaggc gtttggatca atatctcccg tttggctaac
      661 gaaatcattt tcaaataagt cattttgatt tgttcatgtt cggtatctgg tccgcttgtc
      721 acgatttgaa cagctatatc accagtagtg tgaatggacg gaaaaaattt gggagaatag
      781 cctacttctt ttgtatgctg agaattccag tccatcaaaa aacggttttg caaggtatac
      841 accgcttctc cgtaaatacg caaatgattg tctcgccaat aaccaaattt ctttaccaat
      901 cctagatatt catttccaac attgaaccca cctgtatatc cgattttccc atcaataact
      961 acgattttac gatgattgcg ataattgatc cgaggattaa tatacggaac aaatagagga
     1021 aagaaaaatg cgacttctcc gcctgctttt ttcaaattct gaaaaaagct ggaagaaact
     1081 tgtgtggaac cccatgcatc aagaaggact ctgactttta cccctcgtct tgccgcctct
     1141 gtcaatgcat ctcgaacttc ttcacctaat gcatcactgc gataaatata atactggaag
     1201 tggatatggt cttctgcctg cttgatatct tcaatcagcg catcgaattt ttcacgacca
     1261 tctgtaaata aagtgatttc atttttagtc gtatagagcg tactttcaaa taccgtgagc
     1321 atatagatca attgtttcac atctacttgt ccagtaggcg gatgaggata aagccctcgc
     1381 atcaatgcct gtttgtcttc ttcaatttcc aggttcttac cgattttccc ttggatcttc
     1441 aaatcaaaga ttttctcttt ggaaatccct cttcctaaaa aaatatataa aataaatcct
     1501 acaactggaa tgaacatcaa aacgagcagc catgcccatg tttgagcagt ttcctttctt
     1561 tctcgaaaaa cgatgataaa ggaaagcaaa atattcataa tcaaaaggat cgtaaaaaaa
     1621 ttgtcaacga taattttcac ggcttcacct cttcttctac caacttaaga atacccaagt
     1681 tgttttttaa agtaaaggga aaaaagccgc ttccttctat tctcctagcc aataaaaaaa
     1741 gaaagctgga acgaaactgt ccaactttct cattttattt atattgtggt attagaattt
     1801 caagaaacgt ctcatagaaa tgatcgagcc aagtgaacca atgatcacac caatgccgac
     1861 cattaaaaga ttgattttcc aaacgatatt ttgtggatga atcaatgaat aatgggaacg
     1921 taataatgat ggattgatca attggaaaaa ccatgcataa ccaaatgtga tgatcaaaat
     1981 cggaagaatc gcaccgatca gaccgatcca gccaccttca aggaaaaacg gccagcgaat
     2041 atatccattt tttgcaccga ctaaacgcat gatttgaatt tcacgctgtc tagacaaaat
     2101 cgtgatccga atcgtgttcg aaatgagaaa catcgctacg aacagcagca atgccgctgc
     2161 aactaatccc catgtccgaa ctgcaccagc gatttggaaa atccgatctg aggaagctcc
     2221 accgtaatca gaacgagaaa cattcttcaa atctgctgct tcttctgcta cttgttttgt
     2281 ataagaagga tcagtagcac ttactacata cacatcatat aacgggttat tgtcaccttc
     2341 aaacaatttc caggaatcac ccatttcgtt ttggattttt ttcaattcct gttctttgct
     2401 tgaaaattca acttttttaa catgatctaa gtcattcaat tctttttcta gctttttcat
     2461 ctcttcgtct tttgtaccga tatctacgaa tacagataca tcaacgtttc cttctatatc
     2521 ctgagctaat ttagttgcat tcatgataac tgccatgaat acaccgacta aagtcaacgt
     2581 gatcgttacc gcactgactg aagcaactgt catccagccg ttacgtttca aactcttgat
     2641 actttctaat aaatggcgga aaaaggttct aatcatcgta tccatattcc ccttccgctt
     2701 gatctcgaat aatgcggccg ttttcgatag caattactcg gtgccgaatc gtatttacga
     2761 tcgtactgtt atgtgtagcc atcacgacag tagttccttg ggcattgatc cgatctaata
     2821 atttcatgat ttcccatgag ttttctggat ccagatttcc tgtcggttca tccgcgataa
     2881 gtactttagg cgtattcacg attgcccgag caatagagac acgttgctgt tccccaccgg
     2941 ataattcgct aggaaagact cgaactttgt gtttcaaacc aacaaggtcc agcacttcca
     3001 ttactcgttt tttgatatct cgaggttttc tgccgataac ttgcatagcg tacgcaacat
     3061 tttcatatac tgtttttctt ggtaataatt tatagtcttg gaacaccacg ccgatttctc
     3121 tgcgtagata aggaacttcg cggtttttga ttgctattag atcatgacct gcgacgttca
     3181 aagttccttt tgaagctttt tcttctcgat acatcaattt gatgaatgta gatttaccgg
     3241 ctccagacgg accaaccaca tagacaaact ctccttgttc gatgacaatc gagagattgc
     3301 ggatagcggt cgtaccattt gagtacttct tcattacatc cttcatttca atcatggcgt
     3361 tctctccata tctttttcag tctttagcta ttatagcatg gcaatgtttc aacatgcttg
     3421 caaaatctta caattttatt tcaatttaac aatgggttct tattgtagat gcatttttaa
     3481 atagccgtca ataaataaat caaggtcacc gtccataact gcttggacat ttcctgtctc
     3541 tacgtttgtc cgatggtctt ttaccataga atagggatgg aaaacataag aacggatctg
     3601 tgaaccccag ccgatttctt tttgttctcc tcgaagtgca gctgcttctt gttctttctt
     3661 ttccacttct aattgataaa gcttagcttt taacatcccc atcgcttgct cacggttttt
     3721 caattgtgag cgttgtgcct gactagctac tactgttcct gtaggcaagt gagtgatccg
     3781 tacagcagat tctgttttat tgatgtgctg accacctgca ccacttgctc ggtacgtatc
     3841 gattttcaaa tcatctggat tgatttctat atcgatcgtt tcatcaagct ctggcatcac
     3901 atctaccgaa caaaaagaag tatgacgtcg tttcgctgag tcaaaaggcg aaatccgtac
     3961 aaggcgatga acaccttttt ctgattttag ataaccaaaa gcgttatgtc ctttaatcag
     4021 cagggtcaca ctcttgatcc ctgcttcatc acctgcttga taatctagtg tttctacttg
     4081 atagccatgc tgctctgccc aacgtgtata catacgtaaa agcatgctgc cccagtcttg
     4141 ggactccgtt ccgccagccc ctggatgcaa ttcgacgatc gcattgtttc gatcataagg
     4201 tccgtctaac agcatagata attcatacgt cttcattttt tcatctaatg cattgatacg
     4261 tgtttctgct tcttcttgca ttgctgcatc aggttcttct tgcagcatct ccaccatcaa
     4321 ttcgatttct tctagttctt cggcaagaga atggaattgg tcataggttt ctttattagc
     4381 gttattttcg ctgatcactt tttgtgcggc ttcgctgtca tcccaaaaac caggttcagc
     4441 catttttgct tctgcttccg cgatgtcttc ttccagctga tctaagtcaa agagacctcc
     4501 tgaaacttgt gaccttttcc tgcatttctg tcaggagatt tcgaatttcg gatatttcca
     4561 tgttttttcc ttctttcact atactttttt cattatatct agtctaacag aatcagcaaa
     4621 taacagaaag aaggtgagac gaattcttaa aaaaagaatt tgtctcacca atcctgctgt
     4681 cagaaattag tttgcatttt ttccatgaca gttcttatat tttttcccgc ttccacaagg
     4741 acaaggatca tttcttccga ccttattcac atggattggc tgttttttcg cgctagtatt
     4801 actattttct ttggcatctc cgtcttctga gtgagaagct gttccttgag aaacctgttc
     4861 acgctgtacg ttttgacgga tctcagcttt cataaagatt cgagtgactt catactcgat
     4921 ggcgcctacc atatcttcaa acatcttata gccttctgtt tgatattcga ctaatggatt
     4981 gttctgtcca taagcacgta agccgattga ctgacgtaat tgatccattg catcaatatg
     5041 gtctgtccat ttagagtcca caacacgcaa gatcacgact ttttcaaatt ccaacagttg
     5101 ctctggacca tttagttgag ccgctttggt attaaatact tcttgtgctc gttcgtttag
     5161 atactctttg atctcctgag ctgtttttcc ttcgatatct gcaagagtaa tgctatcttc
     5221 atggacaaga gtactgccgg caaaatcgat gatactttcc aaattccaat tttctttctc
     5281 taattgtgta tgcgcatcta ctacacgact gattgtccgt ttgaccatgt tcattagtgg
     5341 cttggataag cttttttctt ccatgattac ttcttgtcgc tgtttataga ttacttcacg
     5401 ttgttcacgc atcacatcat cgtattgtaa gacgttttta cgtgtgtcat agttatttcc
     5461 ttctacacgt ttttgtgcag attctacttg tttcgtcagc atcttgcttt ggataacagc
     5521 atcttcctca ccgatcttca tccgatctaa gaaagctttg attcgttctg aaccgaatcg
     5581 tttcatcaaa tcatcttcca atgacaagta gaactgtgac ataccagggt ctccttgacg
     5641 tcctgcacgt cctcgaagct ggttatcgat acgacgagat tcatgtcgtt ctgtcccgat
     5701 aactgctaga ccaccaagtt ctcttacccc aagtcctaac ttgatatctg ttccccgacc
     5761 agccatgttg gtagcaatcg tcacagcacc tttttgacca gcattcatga tgatctctgc
     5821 ttctttaaag tgatttttcg cattcagcac ttcatgagga atacgttctt gattcagcat
     5881 atctgataat aactcagatg tttcaaccgc aacagttccg actaacacag gttgaccttt
     5941 gcggtagcga tctttgatat cttgcacgac agcgtggaac ttgctttgta atgttgggta
     6001 aagcaaatct ggacgatcat cacggataac cggacgattt gtcggaattt ggtatacttg
     6061 gatgttataa atttctctga attcttcttc ttctgtttta gctgtaccag tcattcccgc
     6121 aagtttcttg tacatacgga aatagttctg gaaagtgatc gtcgccatgg ttttcgtttc
     6181 gtcttcgatc tctacgcctt ctttagcttc gattgcttgg tgaagtccat ctgaataacg
     6241 gcgtccatcc atgatccgac cagtgaactg gtcaacaatc agtacttttc cgtcttggac
     6301 aacataatcg atatctaaaa gcatgatata gttggctctc aatgcttgat ccaagtgatg
     6361 ggtcaatgca gtattttcaa tgtcgtataa gttatccaaa ccgaatgttt cttctgcttt
     6421 ttcgatccct gcttcggtta aactgattgt ttttgattgt acatcgattt tataatcttc
     6481 ttcttctttc aaacgtttca cgaagttgtc tgctcttgta tataaagctg tggatttttc
     6541 tgcttgccct gaaatgatca gcggcgttct cgcttcatca atcaagattg agtcaacttc
     6601 atcgacaatg gcataattca gcggacgttg taccatttga tggcggtaaa cgaccatatt
     6661 gtctctcaag tagtcaaatc ctaattcatt attcgtacta taagtgatat cacaattata
     6721 agcctcgcgt ttttcatccg aagattttga gttgatattc aaccccactg ttaatcctaa
     6781 gaaattataa agttcgccca tttccgtcga gtcacgggtt gctaaatatt cattgacggt
     6841 aactacgtgt accccttcgc cagtcagagc atttagatat actggcatcg ttgcggtcaa
     6901 ggttttacct tcaccagtac gcatttcagg gatatttcca tcgtgaagca cgattccacc
     6961 catcaattgt acgtggtatg gatataaacc taatacacgt tttgctgctt ctcggacgac
     7021 tgcaaaagct tccggaagta attgatctaa tgtttctcct ttttggtatc tgcccttgaa
     7081 ttcagcagtt tttgcctgta attgttcatc agttaatgct gccatttggt cagcgtatgc
     7141 ttcaactttt tttgcaatgc tatctaaacg ttttagttct ttcttatcgt tttcgataag
     7201 tgaacgaatc aaattggcca tgcgttgtgt aatctccttt tccttctgtg tattatccag
     7261 actatgtaga ctttactttt cattttatca ttagttatag ataattgaaa tatcttaagc
     7321 gtattaaagt aagaacttca taatttattc atatttcatc taaaagagga ggaaactcaa
     7381 aaaaacttgc ggcagctgtc agtagctgct gcaagttttc gttgttattt aagtaaggct
     7441 gatcagtctg tttcaatcag tccgtatttt ccatctttac ggcggtaaac gatacttgta
     7501 ccatttgttt ctgcatcttc aaaaataaag aagttatgtc ccagcatgtt catttgtaag
     7561 actgcttctt cactatccat tggtttcaat gaaaggcgtt tcgtacgtac gatatctaat
     7621 tcagaatcat tttcgttttc gtcctcttca ttgaataaag cagcagcatc tgcagtattc
     7681 atgcccgttt cacgggattt acgattaatt tttgttttga atttgcggat ttggcgttcc
     7741 aatttatcaa caaccaaatc gatacttgcg tataaatctg gagaagtttc ttcggcacgt
     7801 aatacaagat aaggtaatgg gatcgttact tccacttttg ctgttttttc tgtataaact
     7861 tttaagttca catgcgccgt tgcatcgggt gcgtcactga aatagcgttc tagtttgcct
     7921 acttttttct caacgtagtc gcgaatcgct tcagttactt cgatattttc gccgcgtaca
     7981 ttatatctaa acataaaaat tacccctttc gtctaccaca atcaagaagg agaaggacca
     8041 ctctttccct cttcttaatt ctattatatc gaactctagg ttatttgcaa attaaatcgc
     8101 tatcaaattt ccacatctaa ttcatctcgc caaagaaatg gttcggatga ttttcggctg
     8161 tgcgacctgc aaacaatctg ctgcgtggaa aagagtctgt cctgttgtat agacatcatc
     8221 aataatcaat atttttttat tttgtatctt agaaaaggtg actgcaagtt caaaaggttg
     8281 aggcatctcc aatctttcct ttcgcgtgtt ttttgcttga gccagcagat gcttctctct
     8341 tttgagtagc atctgaaaag gaattcttgc tgctttcaac atctcgctta cttgattgaa
     8401 tcctctttct gttcgccttt tttcagataa aggtattgga caatagatgt atgtcgggta
     8461 ttcttttttt atctgcgtcc atctatatgc aaaggtgcct gctagtcgga tatctcccat
     8521 aaacttgtat tgattgagcc aggccttcat attttcatcg tacacaaaaa aggcacggtg
     8581 ctgaaaatca taggtcggat atttcttctt ccattcttga cagtcactgc agtaaacggg
     8641 aatagacttt actttctttt tcgtactctc tagattgaaa tggattggtt tttgacagcc
     8701 agaacagcct cttgtttcca ataaatgaaa ttgtgcgcga caaactgaac aagtctgagg
     8761 taatctgtat ttcgaaacac caataatctc gctgaatgtt aaattttgga agatatcttt
     8821 actgcagcaa ctgcacctca cagcatcttc ttcccttctt cattcatttc tttgatttcc
     8881 tgacaaactt ttcgaatagc tgcagttttt tcttcataga aaaagtatac ttgactgttc
     8941 gtataaggcc ctttacgatc ggctcttcct gcgatttgta ccaaagaaga tttagtaaaa
     9001 accggatgat ccgctcctaa aacgataacg gatattcttt caaatgtgac tccacgttca
     9061 aggatcatcg tggttaacag tatgtcatat tttgcttctc gcattttctg gactttttcc
     9121 gcccgctgat tgtctcttga agaaacatct gtgatctcat atctcggaag agcctcttga
     9181 agtgtggctt tcaaacgatg catcatcgtg atacttggac aaaataaaag gatatggttt
     9241 tctttaatta attgatgaat gatccgcacc aaagatttta tcttcttctt gttcttgcat
     9301 ttttgtcgcc aattattcat gaaaaccaag acgggttcag gaagcaaacg ccgatgaaag
     9361 cgtaaagcaa gtttgtggat gtcaaacgtc tgcttgattt cctttagtaa ctttttatca
     9421 ggtgtggcac ttaagtagat gaatcgtccg tctggtttaa tcgctgtttg cacagcatga
     9481 gctaatcctg catccccgtt ataaggaaac gcatcgatct catcgatcac tattaaatca
     9541 aaggaccgat aaaaacggta tagctgatga atcgtacaga taacaaaaga agtaaaggta
     9601 taagccgttg ctgtattccc atggagcaac gtgatttttt cttctggaaa aacataacga
     9661 tagcgtaaaa acaattcatt acaaacatcg actcgcggtg tacaaatcgc tactcttcct
     9721 ccagctttta aagtttccac taaaatagga taaagcatct ctgttttccc tgcacctgtt
     9781 acagcccaaa taagatgatg tcttttttct gaatggcaaa gtattctctt tgagatttcc
     9841 gtttgttttt cagtaagctg tcctgtccaa aaaaagacga cttctcgagg ttttgcttca
     9901 ggctgatcaa acaaatataa atcttgttga gtgtcgcatc gccctaaata tagacaatgt
     9961 gtacaaaaat aaccgtaatc tgcaagctgt ccatcaatga cggacgtcct cattccacag
    10021 cgatgacaaa gaatccagtt gcccaaacgt tccactgctg cgatcttctc ataatttggt
    10081 aaatttacct cctcacagtc tattgcttca atatctctcg tcagaagcct tctaccccaa
    10141 aaatgattca tattcttcgc cctaaaaaaa tatacgaaaa aacaagcgat ctattttttg
    10201 gatcgcttgt tcgatagtta tactgttcta tttattcacc tatgagcgtg attgcttgat
    10261 ctaacacttt ttcgccattc atcataccat aatcagccat attgatgaca tctaaaggaa
    10321 tgccttttgg ttccagacgt ttctcaaaat cagctttcat aaagcgaact tgtgggccta
    10381 ataacaacac atccacgtct ttatttgcaa gattgttatc tgcgtcagaa gcggacacag
    10441 cgaaaatatc cgagtcgatt ccttttgctt cagcagcttt ttgcattttt gttactagta
    10501 aacttgtgct cataccagcc gaacatacaa gcatgattgt ttttttagcc atacttttct
    10561 ctccttttta aaacaaacta tgcaaacgtt ttcttcgcgt ctgttactct attatataaa
    10621 aagaaagaaa tttgtcaacg ttttctaaac gcggtttatt taatcaaata gccaactaga
    10681 tttcactaat ttattgaaca cgacatattc tttgtccaaa ttgatgacaa ctacttcata
    10741 gcctgcattt atccagccat aggcaccttt ttcagggtgt ttataatcat ttgcccacca
    10801 atctgtactt tcccttgctg ttttgggaag tccgttactg ggaaacagca gttcgtcaaa
    10861 ttgggtaaag gtcaacgtta cttgagagcc gccattgttc ttcaaataat gcgtaattgc
    10921 atcgtacttt ttcattcgtt cctgcatccc cagatccacc tgcctttgtc tttattatcg
    10981 catactacag caattgcgca accgttacag tgttgaaaca acctagaact ttatttatac
    11041 tgaataaaga aaatgaaaaa aagaggtgac aagatgatcg agcgctatcg tacaataaaa
    11101 gaaaatggac aaagtgagat tgagatcaaa aaatcacgct ttatctgttc tcttagacga
    11161 atagaatcag aggaccaagc aaaaagtttc attcaagaaa taaaaaaaga acattggaaa
    11221 gcgaaccata actgcagcgc cttcatcatt ggagaaaaaa atgaaatcca gcgaagcagc
    11281 gatgatggag aaccaagtgg aacggcgggc gtaccgatgt tggaagtcct caaaaaaaat
    11341 caactgatca atgtagctgc agtagtcact cgatattttg gaggaacgaa actcggcaca
    11401 ggcggattaa tacgagcata cactcatgcg gtctctcaag cattgtctga cattggaatc
    11461 gtagaaggta ccttgcaaca ggagatctct gtgatgatca cgtatccgca actaggaaaa
    11521 ttacagaatt atcttgaact ccaacatttt tttataaaag aaatccaata tactgatcaa
    11581 gttagcgtca tctgtgctgt agatgaaaaa agtgctgacc actttaaaga atccatcatt
    11641 gagctgctga acggacaagt ccaatttgaa gagggttctg tttcttatca cgggcaactg
    11701 ataactgatc atatataaaa acaggtgatt ctgattctct tttatttcta ataaggaatc
    11761 agggatcacc tgttttttat ttttccaaat aaaactcaaa acgatttcct acgtattgcg
    11821 tacgcacata ctcaaacggc gtaccgtctt caaaataaga aatctggcgc aaacgaagaa
    11881 ctgcatctcc tcgttttacc tctaaatagt cagcaatttt ctcagaagcc aacacagcag
    11941 taatcgtttg attcgctgga ccgatctttt tcccgccttt ttcttctagc gtgcgataca
    12001 acgattcagt aatctctgct ttgctgtagc catcgatcag cttttgtgga acactggcaa
    12061 cttcaaaaca aatagggaca tcatctgcga aacgaatacg ttccattcgc aagatcgtgt
    12121 catctttact caagtttagt ttttccattt cacttgagct tggcgaagtc aagaaataag
    12181 agattgtccg actagacggt actcgatttt gtgattccat aatttctgta aaacttgttg
    12241 caccagacat tttttcttgt actttttctc gcgcaacata cgtacccgaa ccgatctttc
    12301 tttctaagat tccttcatct gccaagtttt gaattgcttg tctcaacgtc atacggctga
    12361 cattgaattt cactgctaat tctctttctg aaggaagacg gtctccgact ttccatatac
    12421 cctgttcgat ttcatttttt aattggtcat gaatctgtat atatacaggt aattgattcg
    12481 acataacgta cacccgtcct ttcgtttctt ctattataac tggtatatac ctgattcaca
    12541 actgaaaaag ataaaggttt ctatccatca acagaggatt tagacacaca tcgttctccc
    12601 tactcctatt gtcagaaaca attttttaga tatgttcaac ctagaaaata acaaaagacc
    12661 ctaaaatcga tgaaaatgcc gacttatgga tcttttgtta ttattagcgg tttttagcca
    12721 tccatttttt tgtatgattc gttgggcgtt ttgattttac ctggaccgct tttccgcctt
    12781 ttttcggaat attcacttca atattgtatt tttctgattt gaccaaatca tataactttt
    12841 cagctgcttc gtctactttt tctttccatt tcatgtggat ccctccaatt cttgtagtta
    12901 acttaactct atcttaattc agcagaatct tcaaggaaca aggtagcaaa gcaaaaaaaa
    12961 gagctctcga actaacg
//
LOCUS       DAHHPA010000075        12882 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.076, whole genome shotgun sequence.
ACCESSION   DAHHPA010000075 DAHHPA010000000
VERSION     DAHHPA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.076"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(109..753)
                     /locus_tag="NBH55_002061"
     CDS             complement(109..753)
                     /locus_tag="NBH55_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1510037.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(847..981)
                     /locus_tag="NBH55_002062"
     CDS             complement(847..981)
                     /locus_tag="NBH55_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCD1510038.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(983..3115)
                     /gene="feoB"
                     /locus_tag="NBH55_002063"
     CDS             complement(983..3115)
                     /gene="feoB"
                     /locus_tag="NBH55_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCD1510039.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(3112..3585)
                     /locus_tag="NBH55_002064"
     CDS             complement(3112..3585)
                     /locus_tag="NBH55_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCD1510040.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            3986..4210
                     /gene="nrdH"
                     /locus_tag="NBH55_002065"
     CDS             3986..4210
                     /gene="nrdH"
                     /locus_tag="NBH55_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCD1510041.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            4369..6528
                     /gene="nrdE"
                     /locus_tag="NBH55_002066"
     CDS             4369..6528
                     /gene="nrdE"
                     /locus_tag="NBH55_002066"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCD1510042.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            6578..7543
                     /gene="nrdF"
                     /locus_tag="NBH55_002067"
     CDS             6578..7543
                     /gene="nrdF"
                     /locus_tag="NBH55_002067"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCD1510043.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(7632..8771)
                     /locus_tag="NBH55_002068"
     CDS             complement(7632..8771)
                     /locus_tag="NBH55_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1510044.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(9080..9793)
                     /locus_tag="NBH55_002069"
     CDS             complement(9080..9793)
                     /locus_tag="NBH55_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCD1510045.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(10047..10748)
                     /gene="nagB"
                     /locus_tag="NBH55_002070"
     CDS             complement(10047..10748)
                     /gene="nagB"
                     /locus_tag="NBH55_002070"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCD1510046.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            10982..12514
                     /locus_tag="NBH55_002071"
     CDS             10982..12514
                     /locus_tag="NBH55_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD1510047.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(12705..>12882)
                     /locus_tag="NBH55_002072"
     CDS             complement(12705..>12882)
                     /locus_tag="NBH55_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1510048.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         4125 a         2605 c         2272 g         3880 t
ORIGIN      
        1 tttgtcaaaa atgaaagaaa tttgcttgtc caaactgaaa aaaacttggc taaatctttg
       61 taaataaaag gtttagccaa gttaatattt ttttaatatt tcacacaatc attctgcaag
      121 ctgatgctcc gggtaaaaaa agtttgtcat acgctgtagt gtatattcat ttccaataaa
      181 atgaacgatg tcccctgccg taaatttagc ataggggcca ggtgaaacaa gcagttcttc
      241 attatgagaa attgcgataa ctgtggcgcc agtcgcctgc cagatattca actcgctaat
      301 tgaacggtcc agatgcattg ctttttctga taaagataat tcaaacggaa gtaaaggatt
      361 catcttatgt attcgcttcg tttgattgac cagctgatcc aataattgag aaaaatgatt
      421 cagctcatct tgttgcttct ccacacttgc catgatttct gcttttgtat cttggatgga
      481 ttgtacatct tgatattgca ataggaaatc ttttgctttt tccttcgaag caacaaatgc
      541 cccacttcca tgccgtactt ccatgattcc aagatctacc agcacattga tagccttgcg
      601 ggtagtctca ggagaagcat tgtacgtact ggcaagtgtt gagcgggcat ggatcttctc
      661 acccacttta tatcggtttt caacgatacg ttcggctaaa tcaacagcaa tctgctggta
      721 gcgcgggcgg acgatttttt tctttccaga cattttcttt cctctttctt tttttacttt
      781 ccctcatctt actatgctct agcaaaataa gcaaaaaagg ggagaatagc ctcttgcaac
      841 gactgatcat cgcttgatct gttccttttt tactgggcaa gctgtttgac agtcatcaca
      901 attcttaccg gattttaaac gagtatagac aattgcgcct gcacttccaa agattaacac
      961 acttaataag aatgtcgcca tcctattcct ctccttctaa tgacaatata gtataaaact
     1021 ggttcttgga ttttggctga cggaacaaaa cataaacgag tcctatcatc agtaaaatag
     1081 caagatacgt ccctaacgct acctgtccac cctccaataa gtgtcccaac tgataaatca
     1141 caaaactaac taaataagct aaaccgcatt gatagcctat ggcgatccaa gtccatttct
     1201 tggcattcat ttcccgatgg atagccccaa ttgccgcaaa acaaggcgca caaagtaaat
     1261 tgaaggctaa caatgaataa gctgctgcgg gagtatatgt gtgttgtagc acttgccaga
     1321 tttctctgcc attttcggat acttccgcat gaccaaacaa aataccaaat gtgccaataa
     1381 tattttcttt agcgaccaat cctgtaatcg ctgcgactgt tccttgccag tttccccaac
     1441 caagcggctg aaaaacaact gccaggattc gcccgaaaaa ggctaagata ctttggtctt
     1501 cagaaacagg ttgagcataa aaattataag aagagctgaa ccagatcacg atactagaaa
     1561 caaagataat cgttcctgct tttttaacaa aagacttgct gcggtcatag gtttgtctta
     1621 ggaccgctga ccattgagga aaatgatatg cgggcaattc catgataaaa ggagctgtat
     1681 ctccagcaaa caaacgtgtt tttttcaagc tgattccaga gaaaataatt gcagtcatac
     1741 caagaaaata ggcagaagga gctacccaag attgatgagg aaaaaaggcc cctgctacta
     1801 aggcaataat cggcaacttt gcagaacaag gcatgaaggt tgtgaccatg atcgtcaaac
     1861 gacgatcttg ctcgttttca atcgtgcgac tggccatgac gcctggcact ccacatcctg
     1921 tagcaatcag cataggaatg aaggattttc ctgaaagacc gaatttcctg aaaagtcgat
     1981 ccatgacaaa agcgatcctc gccatatatc cgcagtcttc cagaaagctc aagcataaaa
     2041 acaatataat caattgcggt agaaatccta acactgctcc cacgcctgcg atgatgccgt
     2101 tcaagatcag ctcctgcatc catcccgcta cttgccattc agctaacaat ctgccaacat
     2161 gttctggtaa ccatgtacca aacaatgtgt cattgatcca atctgtcccc attgttccta
     2221 ctgtttgaat agccagataa tagatcagcc acatcacaaa tgcgaagatc ggcaaggcta
     2281 accaacgatt cgttgcaatc tgatcgattt tgtcactcat cgataattgg aaagaatcat
     2341 ttgacacaac acagagtgta catagttggg taataaattc ataacgttca ttgaccagaa
     2401 tcgtctcact atcgtcgtcc aatagtttct ctgttaactg gatcagttgc tcgatctctt
     2461 tttgctgaga aactgctatc tcgtaatcct ctcttacctt ctgatctcgt tcaaaatatt
     2521 tcattgcata ccagcgtttc ttgccgcttt gttctcctag tacatcttca atttcatgta
     2581 aagctgcttc taaacgtgga tcatatgtac agggaacagg aatagaaggc cgcttttttg
     2641 cagactcctg aatcttttcg attagttttt cgattccttg ctttttcaat acgctgattg
     2701 gtaaaactgg tacaccaaga ccataggaaa gcttttctag attgattttt tttccttctt
     2761 ttttgatgac atccatcata ttcagaccaa tgatcatagg aatgtctaaa tccaataact
     2821 gcgtagttaa ataaagattt cgttctaaat tggtagcatc aataatgttg atgatcagtt
     2881 caggctgttc ctgaaccaaa taatctcttg caatcacttc ttctggtgta taaggagaca
     2941 aagaatagat gccgggtagg tcctgaatat gaatgttcgg attttttttc aacgatcctt
     3001 cttttttttc tactgttacg cccggccaat tccctacata ttgattcgaa ccagtcaaga
     3061 gattgaacaa gctagttttc ccgctgtttg gatttccgac taacgcgata ttcattcttt
     3121 ttcctccctt tgtatcaaga ttagttcggc ctctgacttt cgtaacgtca gttcataccc
     3181 tcgcactgtc agttcgattg gatctcccag aggggctagt tttcggataa ctatttttac
     3241 atttcgcgtc aatcccatat ccattaaccg tcgcttcact gcgccttgtc cgtggattgc
     3301 tacgacgatc ccagtctctc ctgcttttaa ttgatcaaga gaagtccagc tttcagagga
     3361 agcagtcttt ttattgacaa agatttgacc tagtatttcc cgacttactg ccactcgatg
     3421 actttttacc agtaaaatgg cattgttgcc tgataaattg acgacgacga ttttacttcc
     3481 ggcaacgatc ccgatatttt gcaaatggcg ttttgttttt tcttctgttt cgattcgttc
     3541 gacttgataa acgaaattct gctttgcctg cgctaaggtc tgcatattga ttcctctctt
     3601 gtgaaataaa tcatgtatta aaatcattca ttatatgttc taattttctt acgtgttacg
     3661 caaataaatg agaatcattc tcacatagaa tactctcggt aattgagaat gtcaattata
     3721 cttgtgatat tatgagcgaa tctttagtat tgatagataa aaatgagaaa aaaatatttc
     3781 ttcatagttt tccacaattt tttctctatt aaatttgcta ttttcccaca cgatacttgt
     3841 tttcagctct tttttcaaaa acacaaaact tagtattcca cagtttccac aacacaatat
     3901 gtagtgtttg ttgtttgact tttttactaa gagttttata ctatctatta tagataataa
     3961 gaatgtgtta gaggaggcac tcaccatgaa cgtaaaaatc ttttctaaaa ataattgtat
     4021 gcaatgtaag atggtcaaac ggtttttaac agaaaatcaa attgcttttg aagaagtcaa
     4081 tatcgatgaa cagcctgaag cagtagactg gttgaaagca caaggattcc aaagtgttcc
     4141 tgtcattact tctgatgcag aaacagttat cggattccgt cctgatcagt tgcgtaaact
     4201 tgccagctaa catgactaaa agaatcaaaa cgtgatacat ggatttgaag ggcttgagac
     4261 agcaccagtt tttgtcgcaa acttccaaat ccttttttaa agcttaaaaa caatgcaatg
     4321 atttgcaatc aagcatcatc aaatacgaaa tgaaggttgg gcgtacgtat gagtcttaaa
     4381 aatttacaag atgtcagtta tttcaagctg aataatgaaa tcaatcggcc agtcaatggt
     4441 caaatccctt tgaataagga taaagaagct ttagttgcct tttttgagga aaatgtcaaa
     4501 ccaaatacga tgacttttcc atctattatg gaaaaaatca attacttgat cgatcaagaa
     4561 tatatagaag cagaattcat tcaattgtat tcaccatcat ttattgaaaa gctatatcaa
     4621 tttttggacg agcaacattt ttctttcaaa tcttttatgg ctgcttacaa attttattct
     4681 caatatgcgt tgaaaaccaa cgacggttcc gcttatctgg aaacttatga agatcgagta
     4741 gccttcaatg cgctttactt tgcgaacggg gatgaagaat tagcacttgc tcttgcagac
     4801 gaaatgatcc atcaacgata tcagccagct acaccttctt tcttgaatgc tggacgtaaa
     4861 cgtcggggcg aattggtgtc ttgtttcttg actcaagtca ctgacgatat gaactctatc
     4921 gggcgctcaa tcaactctgc tttgcaattg tcccgtattg gtggcggcgt agggattact
     4981 ttatcaaatc ttcgtgaagc cggcgctcct atcaaaggat atgagggtgc tgcaagtggt
     5041 gtcgttccag tcatgaaatt attcgaagat agtttcagct attctaatca attaggtcag
     5101 cgtcaaggag ctggcgtggt ttatttgaac gttttccatc cagacattat tgctttcctt
     5161 tcagcaaaaa aagaaaatgc agatgaaaag attcgcgtaa aaactctttc tcttggcgtg
     5221 atcgtacctg ataaatttta tgaattgaca cgtaaaaacg aagatatgta tttatttagt
     5281 ccatatagtg tcgaaaaaga atatggtgtc ccattctctt atgtaaatat tacagctgag
     5341 tatgatcatc tagttgcaaa tccaaacatt cgcaaacaaa aaatcaaagc gcgtgatctt
     5401 gaaaatgaaa tctcaaaatt acaacaagaa tctggctatc cttacgtaat caatattgat
     5461 acagcgaacc gcgagaatcc aatcgatggc aagatcatca tgagtaattt atgttcagag
     5521 attttacaag ttcaaacgcc ttctgtgatt aacggcaaac aggaatatga agtattagga
     5581 acagatatct catgtaactt aggctcgaca aatatcgtga atttgatgca aagccctaac
     5641 tttggtcaat ctgtgcgtac gatgactcgt gcattgacat ttattacaga tgcatctgat
     5701 atcgatgtcg taccaccgat ccaaaacgga aataagctga atcatacgat tggattaggt
     5761 gccatgggct tacacactta tcttgcaaaa gaacaaatgg aatatggctc agaggattca
     5821 ttagacttta caaatattta cttcatgctg ttgaattatt ggacacttgt tgaaagcaac
     5881 aatatcgctc gtgaaagaaa acaagttttc cataattttg aaaaatcagc ttatgctgac
     5941 ggcacttatt tcgaaaaata cgtaactgga gaattccagc caaaatcaga aaaagtcaaa
     6001 gcattgtttg aaggcatttt tatcccaact gctgaagatt ggaatgcttt gaaacaagca
     6061 gtaatgaaag atggactgta tcatcaaaat cgcttggcag tagcaccaaa tggctcaatt
     6121 tcatatatca acgatacgag tgcaagtatt catcctatca ctcgcatgat cgaagaacgc
     6181 caagagaaga aaatcggtaa aatttattac ccagcgcctt atctatccaa tgagacgatt
     6241 ccgtattaca cttctgctta tgatatggac atgcgtaaag taatcgatgt ttatgcaacg
     6301 gcacaacaac acgtcgatca aggcatgagt ttgactttat tcatgcgttc tgaaatccca
     6361 gaaggcattt atgaatggaa gacaacaacg aagcaaacga cacgtgattt gaatatttta
     6421 agacattacg ctttccacaa aggaatcaaa tccatttatt acgtacgtac ctttactgat
     6481 gatgcagaag aaatcggaag caatcaatgt gaaagctgcg tgatctaata tcatagatag
     6541 attcgcttat tcatatttta attaaaggag cattctcatg tcagaaacat attatgaagc
     6601 aataaactgg aatgaaattg aagatatcat tgataaatcc acttgggaga agctgacaga
     6661 gcaattttgg ctagatacac gtatcccact ttcaaatgac ctagatgatt ggcgtacatt
     6721 gtctcaattg gaaaaagata cagtaggaca tgtatttggc ggtttgacac tgctagatac
     6781 tgtccaatct gaaagtggca tggatcagtt gcgtaaagat gtccgcactc cccatgaaga
     6841 agccgttttg aacaatattc aattcatgga atccgttcat gccaaaagtt attcttctat
     6901 ttttagtaca ctgaatacga aaaaagaaat cgaagaaatt tttgactgga caaatacgaa
     6961 tccttatctg caaaaaaaag ctgagcgtat caatgaaatc tacaaacacg gtacaccgct
     7021 agaaaagaaa attgccagcg tctttttaga aacattcttg ttctattcag gtttttatac
     7081 tcctctttat tatttaggaa acaacaaatt ggctaacgta gcagagatca tcaaactaat
     7141 catccgagat gaatctgtac atggaaccta catcggttac aaattccaat taggattcaa
     7201 cgagttacct gaagcagaac aagaaacgtt aaaagactgg atgtacaatc ttctttatga
     7261 gctttacgaa aacgaagaac gttatacaga agaattatac gatccaatcg gctggacaga
     7321 agaagtcaag acattcctac gctataatgc caacaaagca ttgatgaacc tagggatgga
     7381 tccactcttt ccagatactg ccaatgatgt caacccaatc gtaatgaacg ggatttcaac
     7441 aggcacaagc aaccatgact tcttctcaca agtcggaaac ggctatttgt taggtcatgt
     7501 agaagcgatg aaagacgacg attatttgat tggattggaa taaggattta ttttaattca
     7561 aaaatttttc gaaaaataaa aagagtttag cgtacagata ttttaaatgt acactgaact
     7621 cttttttatg cttattcact tgtttcttcc agcacttttg gactgtctaa cttttccgaa
     7681 acatgttcca ttcttctcaa taaatagatt ttccggatat ttaatattat cgtcttaatc
     7741 actgcataga caggtatgca aatcagcatg cctaatattc ctgcgacact tcctgctcca
     7801 atcaatacca agatgatcgt caaaggatgg atattcattg atttcccaaa taacaacggc
     7861 ttgatcaaat ttccatctaa ttgttggatc actaaaatcg acaacgccat atacaaagct
     7921 tgaaatggag agataaacaa accgacaatg attgctggta ccgctccaat aaaagggccg
     7981 acatacggga taatattcgt gattccgcaa atgaccgcaa gcaacaagct ataaggctga
     8041 tcgaatattc tcattgccaa aaagctcatt acaccaataa tcaaagcatc taagatcgta
     8101 ctgctgatat aggcagataa tgtttcgttt aattctttta ccgtttggcg taactcttta
     8161 cgaatcgctt tcggaaagaa gtgagacaac gcatcaataa atttgtgccc atccttgaac
     8221 ataaatagca aaataaatgg aacggtaaat aacaaaataa aaaattgcat cataaatgaa
     8281 atgatttttg agacacttgc agataaactg actaatatga tattgaacaa attactaaga
     8341 gataaatttg ctgatgctaa ttgttcttcc aaattgaaat ttttaaactc ttcccgctgc
     8401 gccaattcat ttaaccattt gcttgtttca tctgcataca ttggcaagct ctgggtcaat
     8461 tgaattgtct gttccattaa ctgaggcact acattcatga cagttaaaac aactaaaatg
     8521 attacgacca aaaacgtcaa taaaaaaccg aacactctcg ggatccgatg cttttcgaga
     8581 aaaacaacga ttggatcgaa catataatag ataaaaccag caacaagcaa tggtaaaaaa
     8641 attgccgaaa gcattttcaa tattggacta aaaatattcg gcatttgtaa aataaagtac
     8701 acgccgatga tcgtgagtac tagctccact gtccagaaaa ataattttga ttgtttaaaa
     8761 cgtgattgca tacatctcct ccaaggtcct ttgatacttc tattctatca tttaacaaag
     8821 agttacgcgc tgagattttc atttgcttaa aaaaaacaaa aaaaataaac atattttaat
     8881 gagtagacga gtacttaaac aaaaacacgg tttggagtag tgacaaaaat tatactgcag
     8941 tccctttttc caaataaacg gtgttcaaaa gcttgcatct tcgtaataag cccagccaaa
     9001 cgaaaaatat aaggaactat ttttatttgg ctgggcttat tacttgatgt aggacatcta
     9061 ttttacaacc tcagatcatt tattgcgcaa tactgactaa atgcaacttt gtgatatttt
     9121 tgtgtacgga caactcctcg atcacatcgg cataagtgag cccttttgga agatcaatcg
     9181 tatagatatt tttgtacact cggtagtcat caacgaattt tacgtcaaaa ttcacatctt
     9241 cgattacgat atttttctct tcaaaataat cggtaagaaa atctttggtt tcttttctat
     9301 ggataaattg gatttctaac tttttagtcg taggtacatg gatcacgcgt ttcactactg
     9361 tgagtgcaat agcaatcccg ataaaactga tgatcgcaat ggaatagtaa cccatgccga
     9421 tggcgatccc taatccagca attgcccata aagaagcggc agtcgtaagt cccgtcaccg
     9481 attgtttcgt gacgatgatc gttcccgcgc ctaagaaacc gacaccactc accacttgag
     9541 caatcagcct tgcttcatct gaacgaatcg ttccagcaag ctgtggatac tctaaagcat
     9601 ctcttaatgc accactagcg atctcaactt ggatcaatgc aatgatacat gctcccatac
     9661 aaactaaaat atgcgtacgc atacctgctg gacgattttt atattgtcgt tcaaaaccga
     9721 ttgctccgcc aaatagcata gcgatcccta aacgtaaaat aatctctgga attgtcaata
     9781 ccatatcatt catctaaaca ccctccagtc caatatatca tgtctgaaag gagcagacaa
     9841 aaaaagtgca atttttacct cttacgaaaa aaggactgtg acaaaatttt tgtcatagtc
     9901 ctttttatga ataaacggtg ttcaatcagc tgaccctcgg tattactcaa caaacggcaa
     9961 aatatgggga gctatttttc ctttgtcttc tctaatactc aggtcaaaac gctgatttca
    10021 caacctcttt agcctactat tgaaaattac aacttacttg ctgcttcttc atcgatgatc
    10081 acaacaacat tgtcgtgatt ttgcaataca cttgctggca aatgattcgt tacaggtcct
    10141 tcaatcatcc ctttgattgc atcagctttg ctttcgccaa atgccatcaa gacgatttct
    10201 ttacttttca taattgaacc gattcccatt gaaacagctt ttgttggtac atcttctgct
    10261 ttatcgaaga atcgtttgtt tgcattgatt gttgattctg ttaagtcaac tactgaagtc
    10321 gttccgtcaa atgaagcacc aggttcattg aaaccgatat gtccattttg cccgattcct
    10381 aagatttgga tatcgattgg atgtgcatcg atgatttgat catacttttt gcatgctgct
    10441 tccagatctt ctgcttttcc atctggtaca aacgtttctt tgaatggttt tttatcaaat
    10501 aattggacat ccatgaaatg acggtagctt tgttcatctg aaccgcctag tccaacgtac
    10561 tcatctaagt ttacagaagt catttcactg aaatccaaat cgcttgaagt catttcttta
    10621 tataaagtaa ctggtgtgct tcctgttgcc aatcctaata cttttgcacc tttgttcata
    10681 ccttctttga tgatttcaaa tgcttttttt ccgccttctt cagcgttttt tacacgaatg
    10741 atttccatag tgattgctcc tttatatttt ggtatagccc aattatagca ttcttttttt
    10801 ctttttacaa taattagtat agaccaaatt gcattttttt cattttcttg acaataaata
    10861 ggaatccgtg ttaggtttca taagatggtt caatcaaaca atccatgttg catgaaaacc
    10921 aagcaagtga ttcactgtgg acgctttatg tctgcagttt ttttatgaaa aaggagttat
    10981 tatgagtata ctgacattaa acaacattac ccagcaattt ggcgacaaaa tcctctatga
    11041 aggtgtcgat ctgcaattaa atgctggcga acatcttggg ctgattggac aaaacggagc
    11101 cggtaaaagt acattgatta aaatcatcac tggtgagatc ttaccagatg acggacagat
    11161 ccattggcaa aaaaatattc acaaaggtta cctcgatcaa tatgtggaag tcgatgaaac
    11221 attaacaatc gaagaatttc taaaaactgc gtacgcaaaa gaatacgaaa aggaagaaaa
    11281 aattgctttt ctttaccaaa catatagcga gaccttcgat gattccttgc tggaaaaagc
    11341 tgggaaactc caaacggaat tagaccaagg agtgttttat caaatcgaca ccttagttgc
    11401 tgagatgagc agcgggttgg ggatcgatgt cttaggttta gattcactgt tgaaaaatct
    11461 aagtggtgga caacgttcaa aggtgatttt agcaaaacta cttttagaaa acccagatgt
    11521 tctgatctta gatgaaccaa ccaatcactt agatgatcag catatccaat ggctgacgca
    11581 gtttcttcag gattttgacg gtgcgtatat cgtgatttcc catgaccaag aatttcttga
    11641 tcagatcacc acccatatcg cagacatcga gtttggaaag attactaagt atactggcca
    11701 tctaaaacaa gcattgaaac aaaaggaaca aaatcgcgaa agctatttgc gccaatatca
    11761 tgctcaacaa aaacaaatcg aaaagaccga agcttatatt cgaaaataca aagccggttc
    11821 ccgatcaacg atggcaaaaa gccgacaaaa acaattagat aaaatcgaac ggctgacgcc
    11881 tccagcttca gcaagtaaag ctgtctttga ttttccattc tcgcctattg tcacgacatt
    11941 agcagtggaa acaactgatt tagtcatcgg atatgatcga ccgctacttg cacccatcaa
    12001 tttgaccatc cgctttggtg aaaatattgc cattcgaggc ttcaacggga ttggaaaatc
    12061 tactttatta aaaacactga tcggtgagat tgaaaaaata agtggcgatt tccacttccc
    12121 agataacaca aaaatcaatt acttttcaca agatctgtac tgggaaaatc cattagaaac
    12181 accactacaa tatttaagca gtcaatttcc aaaagcgact atcaaagaat tacgtcgcca
    12241 actagctaaa gcagggttag ttaatcaatt agcaagtgaa cctctcacta cattaagtgg
    12301 tggggaacaa actaaggtga aacttgcaca gttgacaatg aaccaaggta atttactgat
    12361 tctcgatgaa ccgacgaacc atatcgatca agaaacgaaa gaaagcttac aagacgggat
    12421 acagaaattt ccaggaacag taatcatcgt ctctcatgaa caagaatttt atcaagatct
    12481 tgtgcaacga gtcattgaga ttgaaccaaa ataaacgttt gattgaatag ttgaaaaaaa
    12541 ggagctgtga caaaagtttt gtcacaactc cttcttctga ataaatggtg gccaattagc
    12601 tgaccttcgg tattagctca aaaaacggca aaatatgagg agctattttt atttggctat
    12661 tcttattact tgatgcagaa cagcttttca caacctcttt tcgtctattt tttattttcg
    12721 agatttctct ttcctttaaa aacaactgtt tgaaagaaga acgcacatcc ttgaaaaaca
    12781 agcccgctaa ccgttacacc taaccagcca tactgctgcc acattgtcgt ccctagcaat
    12841 gaaccagccg cacctccaat aaagtacata aacataaata ca
//
LOCUS       DAHHPA010000076        12769 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.077, whole genome shotgun sequence.
ACCESSION   DAHHPA010000076 DAHHPA010000000
VERSION     DAHHPA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.077"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(104..787)
                     /locus_tag="NBH55_002073"
     CDS             complement(104..787)
                     /locus_tag="NBH55_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCD1510049.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(805..1422)
                     /locus_tag="NBH55_002074"
     CDS             complement(805..1422)
                     /locus_tag="NBH55_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCD1510050.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(1432..2256)
                     /locus_tag="NBH55_002075"
     CDS             complement(1432..2256)
                     /locus_tag="NBH55_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCD1510051.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(2249..2809)
                     /locus_tag="NBH55_002076"
     CDS             complement(2249..2809)
                     /locus_tag="NBH55_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510052.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            2983..3939
                     /locus_tag="NBH55_002077"
     CDS             2983..3939
                     /locus_tag="NBH55_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1510053.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(4244..5200)
                     /locus_tag="NBH55_002078"
     CDS             complement(4244..5200)
                     /locus_tag="NBH55_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD1510054.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            5304..5906
                     /locus_tag="NBH55_002079"
     CDS             5304..5906
                     /locus_tag="NBH55_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD1510055.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(5994..7493)
                     /locus_tag="NBH55_002080"
     CDS             complement(5994..7493)
                     /locus_tag="NBH55_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCD1510056.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7509..8033)
                     /locus_tag="NBH55_002081"
     CDS             complement(7509..8033)
                     /locus_tag="NBH55_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCD1510057.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            8331..9080
                     /locus_tag="NBH55_002082"
     CDS             8331..9080
                     /locus_tag="NBH55_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCD1510058.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9071..9856
                     /locus_tag="NBH55_002083"
     CDS             9071..9856
                     /locus_tag="NBH55_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCD1510059.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            9825..10541
                     /locus_tag="NBH55_002084"
     CDS             9825..10541
                     /locus_tag="NBH55_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCD1510060.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10643..11677)
                     /locus_tag="NBH55_002085"
     CDS             complement(10643..11677)
                     /locus_tag="NBH55_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCD1510061.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(11681..12739)
                     /locus_tag="NBH55_002086"
     CDS             complement(11681..12739)
                     /locus_tag="NBH55_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCD1510062.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         3713 a         2655 c         2311 g         4090 t
ORIGIN      
        1 aaaaaaattt ctcttttttt tgaattctga acaaatagta ctggagtaca aaagacgagg
       61 aagagccact atctgctctt cctcgtcttt tgattctcac ctgttattca tgattctttt
      121 tctcagtttc ccgatatttt tccattttac gactaaaaag ctctccgcta cttggtggcg
      181 tatatgtgat ggcatttgct cctgcttgaa tggtctcagc aatactttct tccgttggac
      241 cacctgtcgc aatgatagga agttcaggat attttttacg aaaatggcgt actgcttctg
      301 cagtttcttt acctgcactg atattgataa tcgaaatacc agctgctaac ttttcatcta
      361 tttctgtata tttcgacgtt accgtactga taactggaat atcaactact cgacaaactt
      421 cttttactgt ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa ccttgcgctt
      481 ctgcgaacat gctcatgtag gaagaacgag caccttgtgt caaaccacca cctacaccgg
      541 agaaaactgg aatgtcagct gcctcaataa tgcttttagt aatcgctgga tgaggtgtaa
      601 aaggatacac agcaattact gcattcgcat ttgtattacg tatgatcgca atatccgtag
      661 taaagatgat cgaacggatc aattttccaa atatcttgat cccgcttgct tctaaaatga
      721 tttccggtac tcttacgata tcttttctca attccgtcat gatttctggc gtaaattgtt
      781 ctgccatcag ccacttctcc tttgctatct ccggtcatac tgattcaatt gatacgtctc
      841 gatgacctgg atgatttttt gcgcataacc ggggtctgtc gcataacctg catcttgcaa
      901 tgcttgtgcg gcttcctgat agttggtggc cgtaatcact ttttcataca gcgcaggatt
      961 ccaatctact ccttggacga acagttgtgt atggtcatcc atggactgtt cccatgaatc
     1021 atacactttg aagtctcctt ggatagttat ccattcttcg ttaacaaatt cctttgtttc
     1081 caggcttact ttcttctgat ccccataagc cttgatcccg aaaagattat tgtatttgct
     1141 agccaaggta ctttttcccc aatttgattc taatatagct tgtcctaaaa tgatactagg
     1201 aagcactcca taaccatctt gtagttcttg ggcatgcgga acaagccgtt caataaattg
     1261 ctgatgggat tgcgcttcat tttcttgctg attatcagta aaaggagaag acagattctt
     1321 caaggaaaaa acaaaggcta agagtatcac tgctacaccg gcaaccactg ctggccaacg
     1381 gatattcttg cgcttttgct ttttccactt ttttttcgcc atgatcaatt cctacttttc
     1441 atttaattca ttcagatatt cttctagcgt caattggtgt ttggtgatat actcagcgtt
     1501 ttctttaccg acataacgta agtgccaagg ttcatatgtg atcttagtta tatcttcgtg
     1561 tccttttgga taacgaacga taaatccata ctgcgcagca ttatctgcga tccatttagc
     1621 tccaggctga tctccgtaac ttgcatccaa tacagtagaa ggataactgt tgtaccagtt
     1681 ttgatcgacg acatcgactg ccagacctgt gtggtgttcg ctgtatcctg gctccgtaat
     1741 cgtttcttta gtaatctttg tcgcctcctc ttctgataca ccttgacgac tcatcacatc
     1801 ttggacattt tgttcgaaaa cttgttgttg ggatgataca gaccggtaag ctgagaccat
     1861 cactaatgga aatccagctg cttcagcagc cttggcaaat gcttcatatt ctggagcaat
     1921 gcgtttgtcg atcaagtatc cgttacttaa tgatgccaat tgagtagact catcaatttc
     1981 tttttctaat ttgtgatctg gaccaaccaa aactagcttc caatcgtctg ttttaacatc
     2041 tggcaattgt ttggacgtac gatcttttgt ttctttgtcc tttaccttgc tgctggttgt
     2101 ttgactgttc ttttctttac tagcagttgt tttggtgctt gttcgttcga ctgcttgatt
     2161 gccggcttga acatgcgaat catcatcgct aaaaataaca tagcccattg ctgcgatcat
     2221 aatcacaact gctccaaatc ctaaaaattt attcacttga aaaatccttt cgttgttctt
     2281 cacgttcccc attcgcctca tgaacgtaag tttcttccag attttcattc acccaatcga
     2341 gaagttcgat tttttctcct tcgatctgtt tttgaacatc tgctggcaga cgaaaattca
     2401 aaatatcatc atagccccat tcatcatatt gaatacttac tttcaagtca aggtatctac
     2461 cagcctgctg attatttttc aaagggatca gctcgacttt tgctgctttg ctgctaagcc
     2521 atttttgcgc gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg tttcgttttt
     2581 cgggaaaaat aatcgtttga cgcaaaatct tttgcgttag catccattca taatcagtga
     2641 aaagattttt tactttctgc atcgcttcag atggcagatt tttctcgcca tctttccatt
     2701 tttgccattg ttctggactg atcagcaagt attcatctaa aaaatttttt tcagtttcaa
     2761 actgttctaa taatacactc ataataagat caatatatac ttgatgcacg tttatcccct
     2821 ccttttaact attctactta agttttgatc aaaaagcacc tcttaaatcg ctgctttcat
     2881 tttttttaat gtttcaagtc ctctcctctt tattcttcgt gatttttgtt ataataatag
     2941 acagacaaga aactcaatcc aactatgaag gagcgttttc agatgaaaaa atatcaatgg
     3001 ggaatcgttg gtcttggtac catcgcacat gagtttgccg aaagcttcaa tcaagagacg
     3061 agcgaactaa cagcagttgc ttccagaact tcagaaaaag cggaaaactt cgctcaccgt
     3121 tacaatattc caaaagctta cggaagctat caagaaatgt tagacgatgc tgaaatcgac
     3181 atcgtgtaca ttgctgtacc aaatagacag catatcgacc atatcttggc agcattggaa
     3241 gcaggcaaac acgttctatg tgaaaaagcc atcacgatga ataaaaaaga actggctgac
     3301 gctatgcgct tagcagaaga aaagaatttg atcttagcag aagcgatgac aatcttcaac
     3361 atgccacttt atcagcagtt acgctcaata atggatacag gaaaactggg ggctttaaaa
     3421 atgatccaag cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg gttcttcaat
     3481 ccagaattag ctggaggagc tctgttggat atcggtacgt atgctgtatc tttcgctcgt
     3541 ttctttttga gctctcaacc tgaagtagtt gcctctacaa tggttccttt tgaaacaggc
     3601 gtggacgaac aatccgtcac tatcttgcgt aacaaagaaa atgaacttgc tgctgtcagc
     3661 ttgacttttc aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa cggttatgtg
     3721 acgattgctg attacccgcg tgctgatcgt gcggagatct tatttacaga tggtacaaag
     3781 gaattcatcg aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa gaatatggta
     3841 cacatgattg aaggtgactt gcccaatcgc tctctcttct tgacaaaaga tgtcatcgaa
     3901 atcttggatc aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt tgtgaaaaag
     3961 ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta tttttcgttt
     4021 ggctggactt attaccgcag aggaaagctt ttgaacaccg tttaagaaag aggttgtgag
     4081 cctaccgttc agctccgagt cacaaggagc aatttcaaaa aacagcttct catgtttgtt
     4141 gaatattgtg acttgtgacc gaggagttga caggagaaca ccgtttaaag aagaggttgt
     4201 gaaatcagct ttttgacctg aatattagct gaattcacaa cctctatttt tggatttttt
     4261 gttgggagaa ttctgccttg atttcaataa aacttttaaa agactgcaaa aactggaaca
     4321 attctgctcg atcctcgaat tcagggcgac tagtcggcag aggcatttgc cgatacaata
     4381 cataaacttc ttcaatacgt gttaaaattt gtctgccatc atttgactct gaaaatgttt
     4441 ctgcagtata aatcaataaa ccatagatat gttttccata ataagctggt tgctggattc
     4501 tctctagatt ttccagcatg tctttaatca cattggcctg tgctcttctc atagagaagt
     4561 aagtttcgta gtatagcggc tgtctgagcc agtagttttc ttgatgttct ctggcatctt
     4621 cttggctttc gcgaatgaat gtcagtaaat gttcgcattt gatctgcaca tcttctttat
     4681 tttctgataa aagaaattct gccatttgtc tcaaaatatt tcgaaattct ttttcgatct
     4741 gtatttggtt ttccttcagc tgcttcgtac gatctggcat gtaaacattt gcaagcaaag
     4801 caaatactaa tccaatcgcc attaatcccg cttcattgcc gatcagctgc caagacatgt
     4861 tttcctccac taaataatga gtgaccaaaa ccgaatttac tactatccct tcggttaatt
     4921 tgaagcgaga ggcaagcggg atgaacagga ataaaaaaag actgaagcca aaaatagtat
     4981 acccaacagc aaaaaaactg aacagtgctg ttgccgtagc taatataaag gctgcgattc
     5041 gattcatgcc cgtacgcaat gtagagcgct tggtatttcc tacacttaat aatgcaatga
     5101 tccctgcagc tggccaatac aacagcgata agctacccgc tgtgaccatc gaaaagaaga
     5161 cactgatcac tgttttgact gttcgcattc ctatacgcat ttacttcccc cttttttatt
     5221 ttaattatac ttgaataatg aaaaactagc tatcagacaa aagtttaggt atgatagtaa
     5281 aaagaccaaa aaggagaatc tctatgcaat ttggccgttt tcgactaggt atgcgtacga
     5341 tcaaaagtgc cttagctgtt tttttatgta tcttgttttt tcatttgact caacgaggaa
     5401 tcccgatgat tgctgcatta tctgctgttt tttctttgcg tcaggacttg actacaaccg
     5461 tttcttttgg aaagtctagg atcatcggca atacattagg tggtacatta gctatcattt
     5521 atttttttgt caaaagctat tttcacaatg attttttggt agaattgatc ttgcttccta
     5581 ttctagttat tattgtgatc gtcgtgtcag atggtattaa caataattcc ggtatcattt
     5641 ccgcaatagc tacgttgttg ctgattgcgt tgagcatccc tcaaggcgaa tccgctttgt
     5701 atgctatcca acgtgtacta gatactttta tagggacatt cattgcaatc ggtatcaatt
     5761 tctttcttcg tcctcctgaa acagaaaaga aggaagaact atcagaagat ttggtcgaac
     5821 tccaaaagaa agaacgattg ctaagaacaa atttacaaga gatagaagct aagatccaaa
     5881 aacaaaagaa aaatagcgag gactaatttt cttttgcaat aaaataaaaa aatggagaga
     5941 agaactttaa aaagtcttcc tccatttttg attgaacggt gaagtgtact actttattct
     6001 tccttcatcg agttttgttc tgcctctttt ggcgcttcat acatatttac ttggctagta
     6061 gtcccacgtg tcgacaaatt ctctacgatt tccttcaagt caagtcctgt tgtcgctttt
     6121 agtgtttctt gagtagaaga aagcaaattt gtggcataat ttgtcactcg gttcgctcca
     6181 ctggtttctc ctgaactgct tcctgtatcg acaacagaga ttttttcgat atttcctagc
     6241 ggctgtgctg cttcgcgcat caattgcggt agcatatcga taaccatact caagacagct
     6301 gcttcgccat attccttgaa ggcattggcg atcttttgtt ttgcttcggc ttctgctttt
     6361 ccttttgcta gtgctgcttc ggcttctgct tgtccagcta aacgtgtctt attggcttcc
     6421 gcttctgcca gtgcttccac acggaatcgt tctgcttcgg cttcagtcac ttctttgact
     6481 ttttgagctt gtgcttcttg ttctttagcg taccgatcgg catctgcttt tttcttcact
     6541 tccgaatcat attgcttttc acgacgtgtg atttcttttt cttccagttc gatttgtttt
     6601 tggcgttcta cgattttaat ctgcatttcc tgttcgatta cttgttgttg tgctcgggca
     6661 ctttctaaat tgtaggcttg gtctgctttt gctttagcaa catcttgttc ttgtttgtag
     6721 gttgctaatt tcagttcttt ttcttttaat gattctgcga tctctgtttg tcgctgtaat
     6781 tcagctgctt gtgattcttt ttctgcttga gcttttttga tacgcgtttc ttttagagct
     6841 tccgcttctg ctatatctgc atctcttttg acttgggcga ttcttggttt ccctaaagag
     6901 tcaagataac cgtttttgtc tctgacctct ttgatagtga aagaaacgat gatcaacccc
     6961 atttttgcaa ggtctacaga agcgacttct tgtacgcttt gactgaactt atcgcgattt
     7021 tgatagattt cttctacagt catagaacca agaatcgagc gcaagtgtcc ttctaatact
     7081 tctcttgctt cattttccag ttcttctctt gtcttgccta aaaactgttc tgctgctgta
     7141 gcaatctctt ctacagatga accgattttg atgatcgaag taccatcaca catcaccggt
     7201 acgccttgtt ccgtatacac ttcaggggtg gagacgtcca atttactcga taatagactg
     7261 atccgattcg aacgttgaaa aactggcaag acgaatgcgc cacctccacg cacaattttg
     7321 attttgttat tgctttcatc aatgtgcaca tttttgcttc ctaaataact tccactgatg
     7381 atcaacgctt catccggttt tgctgtctgg tacttcgcta caaagacgat cagcaacatc
     7441 agtaaaatca aagctgcgat tgcgatcgcg ataaaaacaa ctggtatttc catttaacac
     7501 acgctccttt aagcttttat catgctttca taaggcatga cataacagat tctttccttg
     7561 atctcaatga tcaatacttt cgttccccgc tcagctgtct gttcttgggg gctgtaaaaa
     7621 caagctggac gactaatgga accggtaaca cttttcaact gaatttctcc catacctcgt
     7681 acagggatcg gtgtgatcac aacagctact tgtccttcca gagttttttc agaagacgac
     7741 atcgttgatt cggcatgttt catcggcatc aatatataga aattaagcaa aaataccaac
     7801 actatcgata tgcctccact agcaataaat aaaaataaag atgattgatc tgttaaacgc
     7861 tcacctagaa aaccaaataa gcttgtgaaa gttaaccaag ggataataag catcggatca
     7921 agcgggccat caaaatcgaa tacatctcca aaaataatca ataggactgc caatcctgca
     7981 caaatcagca atgtatacca ataaatcgtc tcaagtgcga ttccccctat catatacacg
     8041 cctccttcca tttctacgtc tatcatacca atgtttgaaa attctgtata gcttctttta
     8101 ccgactcagt ggtgtttttt ataggaaggt tgtgtttatc gttgacaatt tgttaaattt
     8161 ttgttttcat gttaaaattt tcacaataaa ttctttcgag gtagcctatg tttccttttt
     8221 ttaagaaaaa accattaaag ctccagaaca cgatcatcct tgtcgtttgt ttctccacat
     8281 tcgtttcttt gattgtttct gccatattga tcaggaattt tattctttcg atgaaccgat
     8341 ctagtgtgtc agataaaatc tctagcattg ctaaaatcgt tgccaatgat aaactcatcc
     8401 aaaaaaagct gcagactggt gacccgacac atgcgattca aggaataaca aatgaaatca
     8461 tgattgcatc gaatttagat tttatcgttg tcatggaccc tgaaggcatt cgatattctc
     8521 acgcagaccc aagttacatc ggtcttcctt tctcaagtca tgaagatgtg aaaaaagtga
     8581 ttggaggaca agaatttttc tctgaaaaag aaggaatgtt agggttcggc ttccgctatt
     8641 tcgtccctat ttattatgat caagagatta ttggtgcagt ttgtgttggc ttaacaaaca
     8701 acacgattga caatcaagtc aaagatggac aaaaaatgat tgttttttca ttgattattg
     8761 gacttagcgt aggcatcata ggcgccgttt ttcttgccca aaaaatgaaa aaagtactgc
     8821 ttggacttga accacaagaa atagcagaac agttaagtga aaaaacgatt atcgaaaatg
     8881 aagtagatga aggaatctta gccatctctt ctgataaaac tatcctcttg atcaatacac
     8941 ctgcaaaaaa cattttctac caaacagagc agctattgga tttcgaagta ggaaaaaaat
     9001 cagtgataat ctatatgacg ttttgtttaa agagacgatt gagcatcaaa cgaccgttcg
     9061 gactcaatcc atgtatttaa atgggataga aactatttct agcgttacac ctctttttag
     9121 caatgaccgt tttttaggag cagtcgcaac tttacgaaat cgatcagaag taatgaaatt
     9181 aatggaggag cttgggggga cgaaacaata tataaactcc atgagagctc aaacccatga
     9241 attcatgaat aaacttcatg tcatttctgg tttgattgat ttgaagaagt atgaagaagt
     9301 cacccatttt atccagcagc taaatcaaaa ttataaaaaa gagattggcc aagtctcaag
     9361 tctgatcaaa gtacctgcaa ttgccggttt tttattaggc aaaataaacg aagccaaaga
     9421 acaatctgtt gaaatgctgt tagaaagcga ttcttttatc cctaaaatgg agatggatga
     9481 ctccatccat tcacttttgc aagtgttagg gaacttactt gataatgcaa aagaagccgt
     9541 gctacaaaaa catgaaagaa acggacaggt ccggattcat ttaaactatg acacagaagc
     9601 tgaagtattc atcgtgaccg tctgcgatgg cggtgtcggg atatcagaag aaatctcaga
     9661 aaaaatattt acgctaggtt tttcgacgaa gggaatcaat cgtgggtttg gattgaatct
     9721 catccattca atcgtcaaac gccacggtgg atttttagaa ttttccaaac aaacaaatgg
     9781 cggtacaatc gtctatctag aattaccaaa aaaggaagat caaaatgatg acaaatgtcc
     9841 tgataatcga agatgatcca atggttgcag aattgaatcg tacttttgtg gaaagcatcc
     9901 ctaatatgaa cgtaatcgaa agtttcagaa atgtagaaga aggaaaaaaa tatttcagac
     9961 aaaaaaacgc agatctgctg ctgcttgatg tatacatgcc tggcgaaagt ggtatccatt
    10021 ttttatcttg gcttagacag caaggaaatg atatcccagt gattttgatc actgctgcag
    10081 atgatatcga aaccataaag aatgtgatcc gctacggtgc cttagattat ttgatcaaac
    10141 cgttcacttt gcaacgtttt cagctggcga tcgaaaagta tcttgctttc tctcagacaa
    10201 tccaaacgac tgaaaaagcc gatcagaaat tgatcgatca agtattcaac cacaccactc
    10261 ccattcacga aataccatca ttcatcgatt tgcctaaagg tctgtcaaaa ctgactttaa
    10321 aaaaaataag tgcatttatt ctaaaagaag atactatttt ttcaacaaaa gaactggctc
    10381 aaaaaagctc actgtcacgt atatccgtca agaaatattt gaatttttta gtagaacaag
    10441 atatattgaa cgaaagaatc aaatatttgg aaatcggcca gccgctaacc tgttatgaat
    10501 tgacaaagaa tgggcggaaa aagctcacag attactgttg agtcatcgaa ttatcccata
    10561 caaaatagtc caagtataca gaagtaacaa ctgtttactt ggactatttt taaacatttt
    10621 attgacgaat atccatgttt tcttatctta attgacgggc tttaggaaga gatacctcat
    10681 ttttcgtttt tccagtttct attatttcac gaaagttctc gaaacttgtc gaaatcatat
    10741 ttgataatgc ctcatctgta tttgaaccta catgcggggt tacaagaact ctagggtaca
    10801 tttctactaa ttgctggata gcagggtctg gaatcgtctc ccatggcttg aaggatcgga
    10861 aaaaaacctc tgtttcattt gcaaaaacat cggttgcaaa accagctaaa tgattgcttt
    10921 tcaaagctct taaaatagct tcattatctt gaagctctcc acgggctgta ttaatcaaga
    10981 tgctaccttt tttcatttga gctaaaaatc cttcattgat catttgatcg ttttcacctg
    11041 ggatatgagg tacatgaata ctgacaatat cgcttttggc taataactct tcaagcgagt
    11101 ggaacgtcaa tactttttta gcagcttctg tctgatgaat atcgtagccg atcactttgg
    11161 ctcctagtcc cttaaacaaa acagcttccg tcaaccctat cctgcctgtt cctatgatcc
    11221 ctactgtgca atttctgatt tcacgactaa acatttgctc atccactcga aaatctttga
    11281 aagatgttcg gatcgtcgta tacgcagtat ttctcaaaag catcatggca aaagtcaaag
    11341 ataactctgc tatcgcattt ggcgaatacg atggtacacg ggctactgtc ataccaaaat
    11401 cagctgccgc ttgtagatcg atatgattga ctcccacagt ccgtgtaaaa acataacgaa
    11461 tcccatattc ctgcattttt gcaagattgg tctcatctgc tatacaattt cccctcaaca
    11521 atacagcatc atggtccttt gctgacacga tattgtcatg tgtcaacagt tcttcataaa
    11581 gactgagatc ataatggtaa atattcaatt tttcaaaaaa atctatctcg ttcggacgaa
    11641 caccgtaaca tgcgattctc atactttctt cctccatttc ttatgaaaat aaacctatgc
    11701 catgaatcat ttgtaataca acgatccata caggtgtcag taccacagca aaaatagttg
    11761 acagtaagga tgcattggag gctaaaagtg cctccctatc aaaactgata gcataggctg
    11821 ctgccactgt tgcagctggt gtagccatca tgataacgat tgttgccagt gcttcaaaat
    11881 tgactggtag aatattcgtt gccgtcaaaa ctgctagaat cacgatattc aatgcaggta
    11941 caaataccac tttgactaga ctgtaatacc atgaagtttt ttcagatgca gcttttttga
    12001 agcttacttc acctaatgtt gcgccgattg ctagccacgc caatggtgaa cttaaaccag
    12061 ctaaataatc catcggctga aatagccacg gtgcagtctg gtcaattcgc aaaaacgcta
    12121 cttggcttat ctctccttct gcacctgtaa ctgacacttg tggcagatac ccttggaaca
    12181 gccaaatgaa aaggccggca aaagtagcga taatgattgg attgacgaac atcgctttga
    12241 tatttttagc ttccatcttc aagccactca tttttatgta tccataagaa tacaaaaaga
    12301 ttcggtagcc aatattgaaa attgaggaat acatcacgcc gacaggacca taaatcgcac
    12361 tgacgatagg cgttccgaaa aaggtcgtag aaccaaagat cgtcaggaca cgcaacgttg
    12421 tttgcttatc ccctttatag cttaagaaaa aaggttttga aactagaata agaataatat
    12481 aaataactaa tccccaaatc aagacattga ttccttgcat cagtaattct gtattgatat
    12541 cttgcatgaa agctcgaaat gccagtgctg gcaaagccac tgttaagacg actttcgata
    12601 gaatttttcc aacagattca gaaaaaatcc ccttttttct acaaacaaac cctaataata
    12661 taataaaaac tgttgaagtg attgcactga caatcgacat atctgtcaaa gtcgatcgaa
    12721 aaatgtcaaa taagttcatc tttctcctcc taattatgtt catttattc
//
LOCUS       DAHHPA010000077        12412 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.078, whole genome shotgun sequence.
ACCESSION   DAHHPA010000077 DAHHPA010000000
VERSION     DAHHPA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.078"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            251..526
                     /locus_tag="NBH55_002087"
     CDS             251..526
                     /locus_tag="NBH55_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510063.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            539..2611
                     /locus_tag="NBH55_002088"
     CDS             539..2611
                     /locus_tag="NBH55_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCD1510064.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2776..4662
                     /locus_tag="NBH55_002089"
     CDS             2776..4662
                     /locus_tag="NBH55_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD1510065.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4674..5621
                     /locus_tag="NBH55_002090"
     CDS             4674..5621
                     /locus_tag="NBH55_002090"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD1510066.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5641..6168
                     /locus_tag="NBH55_002091"
     CDS             5641..6168
                     /locus_tag="NBH55_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD1510067.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6231..6884)
                     /locus_tag="NBH55_002092"
     CDS             complement(6231..6884)
                     /locus_tag="NBH55_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCD1510068.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7016..7858
                     /locus_tag="NBH55_002093"
     CDS             7016..7858
                     /locus_tag="NBH55_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCD1510069.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8016..8912
                     /locus_tag="NBH55_002094"
     CDS             8016..8912
                     /locus_tag="NBH55_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCD1510070.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8915..9628
                     /locus_tag="NBH55_002095"
     CDS             8915..9628
                     /locus_tag="NBH55_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCD1510071.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9648..10166
                     /gene="msrA"
                     /locus_tag="NBH55_002096"
     CDS             9648..10166
                     /gene="msrA"
                     /locus_tag="NBH55_002096"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1510072.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10163..10390
                     /locus_tag="NBH55_002097"
     CDS             10163..10390
                     /locus_tag="NBH55_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCD1510073.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10542..11093
                     /gene="lepB"
                     /locus_tag="NBH55_002098"
     CDS             10542..11093
                     /gene="lepB"
                     /locus_tag="NBH55_002098"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD1510074.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11176..11676)
                     /locus_tag="NBH55_002099"
     CDS             complement(11176..11676)
                     /locus_tag="NBH55_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCD1510075.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11955..12353)
                     /locus_tag="NBH55_002100"
     CDS             complement(11955..12353)
                     /locus_tag="NBH55_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1510076.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         4312 a         2122 c         2487 g         3491 t
ORIGIN      
        1 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
       61 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
      121 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
      181 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
      241 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
      301 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
      361 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
      421 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
      481 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
      541 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
      601 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
      661 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
      721 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
      781 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
      841 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
      901 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
      961 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
     1021 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
     1081 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
     1141 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
     1201 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
     1261 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
     1321 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
     1381 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
     1441 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
     1501 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
     1561 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
     1621 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
     1681 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
     1741 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
     1801 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
     1861 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
     1921 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
     1981 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
     2041 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
     2101 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
     2161 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
     2221 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
     2281 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
     2341 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
     2401 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
     2461 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
     2521 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
     2581 tcgagcaaag tcaaaaaaac gagtccaata aaataaaaag gtaaaaattt tcaagtttta
     2641 taaaaaacaa aacatatcag aacaggatcg aatcggtcct gttctttttt tgagaacaca
     2701 aaaagaacaa ataccaaaaa aattttcctg aagttcacta tttttatgat acaatatcca
     2761 agataaggag ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt
     2821 gaaaaagatt tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg
     2881 attgggacga acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc
     2941 agcgacggtg atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct
     3001 caagcagctg aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc
     3061 cccttgatcc aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga
     3121 gaagaccctg atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat
     3181 gcttggacta cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta
     3241 gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg
     3301 ttgattgatg aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa
     3361 gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc
     3421 cacgatcgtt attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa
     3481 ttatacgagt ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt
     3541 gtagaagtag aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg
     3601 agggctggcg tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat
     3661 ctaaaggaga acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca
     3721 caacgtttag ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa
     3781 acactgctag aacatttaga tttattgatt caatctcggg aaagactagg tattactggt
     3841 aaaaatggtg cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat
     3901 agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa
     3961 gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa
     4021 acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga
     4081 tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg
     4141 ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg
     4201 gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc
     4261 actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa
     4321 ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa
     4381 gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa
     4441 aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca
     4501 gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc
     4561 actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa
     4621 atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac
     4681 aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa
     4741 caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt
     4801 ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt
     4861 ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg
     4921 aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt
     4981 ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc
     5041 ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac
     5101 acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa
     5161 tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg
     5221 catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat
     5281 tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc
     5341 taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa
     5401 caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc
     5461 atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt
     5521 tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat
     5581 atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa
     5641 atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt
     5701 ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta
     5761 gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg
     5821 attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg
     5881 gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca
     5941 gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt catccatcat
     6001 gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat
     6061 actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag
     6121 cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga
     6181 tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag
     6241 aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca
     6301 tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg
     6361 cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc
     6421 acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc
     6481 cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat
     6541 acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt
     6601 cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt
     6661 gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca
     6721 tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga
     6781 accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact
     6841 tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta
     6901 ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt
     6961 acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac
     7021 aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt
     7081 aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc ccgatgatga
     7141 ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac
     7201 aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag
     7261 tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca
     7321 agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg
     7381 attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa
     7441 aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac
     7501 actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt
     7561 tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg
     7621 gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt
     7681 gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt
     7741 caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc
     7801 ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc
     7861 gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa
     7921 attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa aatttttgat
     7981 aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa cgacatttga
     8041 tcctcgtttt gttcccatta ttaattgcga tcatcatcta tgcaggttta gctgcttcta
     8101 taccgaaagc tggcccacta atgaaaccag taactactgt gagagaagat ccaaataaaa
     8161 aagaaaaact tcactacata gctatcggag attcgctgac tgaaggagta ggagatcaaa
     8221 ccaagcaagg aggattcgtt ccccttgtcg caaatgatct aaaagaccgt tataatttga
     8281 ccgcggttga aacagaaaac tatggtgtaa atggtgaacg aagtgaccag atcttaaagc
     8341 gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac agctgatttt atcacactga
     8401 ctgttggcgg taatgatttg atgaaagtta tccaaaataa tttattcggg ttaacggtca
     8461 attcctttaa aaagccactg aaaaactatc aggaaaatgt cacagaacta ttggaagaat
     8521 ttcgatccct caataaaacc gcacctattt acattttagg catctacaat ccattttatt
     8581 tgaactttcc tgagatcact gacatgcaga caattgttaa caattggaac aaaggaacag
     8641 aaacggtgac aaaaaaaatg ccgaactgct actttatccc aatcaatgat ttgctttatc
     8701 aaggattgaa tcaggaagta ggtgtcacag aagaaggaac agatcaaaca actagcacga
     8761 cagaaaatct aaatggcatc aaaaataatg tgctctatga agaagatcat ttccatccaa
     8821 ataatttagg ttatcaattg atggcaaatg cagtgagaga tcagctgatc gagacacaaa
     8881 accaatggtt agagaaggag gatgatcaat aaacatgaaa acaagatctg acgtatcttc
     8941 aaacaaaaca aagaaaacaa attactggaa attcgccttc cttatccttc ttggggtagt
     9001 acttggttcg accgtatttt taggcagccg tatttttgct aatcgggaac cagaactccc
     9061 agaaatacca gctcttacgg aaagacaagg agatcctgtc ttaacgatca attcaaataa
     9121 agaaaaagtc aatcaaatca tcagcttttt ccttagtgag tatcaaaaag atagtgatat
     9181 tgaatataaa ttttatttgg aaaatgaagc actactcaat ggtactttcg aagtactagg
     9241 ttttcctatc gatttctatt tatattttga tccgtatgtg atggaaaacg gaaatgtcca
     9301 actaaaagct agaagtcttt ctatcggaac attgagtttg ccaatcaaag atgtgatgaa
     9361 tatgatcaag agaaactata aattaccaga ttggatcgaa gtcaatacag atgatctgac
     9421 tatcatgctt aggttggatc aattccgaat gcaaaacggg atgtatatta aagcagataa
     9481 aatcgactta gtaaatgatg acattcggtt tagtctatat ttgccaaaag atacctctga
     9541 tgcttcaaac aactcaaata ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa
     9601 agaaaataaa aaatcatcgg atgagtagta ttagaggggg aatcataatg gaacgagcaa
     9661 tatttgcagg aggctgcttc tggtgtatga tccagccttt cgacacgctt cctggtatcc
     9721 atacagtgat gtcaggatat actggagggc atgtaccaga tccgacttat gaacaagtaa
     9781 aaagccaaac gactggacat acagaagcag tggagattat ctatgatcca acattgattt
     9841 catatgaaca attagtagaa ctttactggc agcaaactga tccaactgac gctttcggac
     9901 aattcgagga tcgcggtgac aattatcgcc cggtgatctt ttatttgaca gaagaacagc
     9961 ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag tggacgtttc gaacagccaa
    10021 tcgttacaaa aatcgaacca gcccaaccat tttatctagc agaagaagac catcaagagt
    10081 attataaaaa gcatcctgaa aattttaaaa gaaaccatgc acgccgggct gcctttatcg
    10141 aagaaaattg gaggaacgaa gggtgaatcg aagctttttt cattatttaa tgacactgcg
    10201 aggcggtggc acacatgatg ctttatctgt tttcgctaca gaagccggta aagacatcca
    10261 atttcctaag cacagctcta gctacgaaga aatttctaat tatctggaac tgaatgtcga
    10321 ctatttgccg tccatggata tctttgacga agcatgggaa aaatatttag aaaataatca
    10381 tgacatataa gagatagttt tgtactttat gatcaaaaaa gttttttgtc caatagccta
    10441 attgaaagaa ttatttttat ttcagctatc tagtcgcatt tttttgatat ttttcttata
    10501 ataaagaaag aatgaaaaaa agagggatgg attgatagag attgacaaaa aaacaacgct
    10561 atattaatcg cttctggctt gtattcaaat acttacttgt ttctgtattc cttgcgttca
    10621 tgttgcgggg atttttattc atcccggtac cagtggaagg caattcaatg gaaaacgtgt
    10681 tgaagcaagg tgatatggtt gtgatggaaa aattttcgga gatcagacga tttgatattg
    10741 ttgtttttca attagcagat ggcacgattt atatcaaacg agtaatcggt cttccaggag
    10801 aaaatgtcag ctaccaaaat gatcaattga agatcaatgg gaaagtagtt aaggaacctt
    10861 atctaactaa aaacctgaaa tcagatcatg caaatgcatc ctatacgacg gactttacac
    10921 tacaagagct aacgggacaa tccaagcttc cggaagataa ttattttgta cttggagata
    10981 atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat caataaaact gatattctag
    11041 gtaaagcaag atttgtctat tatccacttg atgaaataaa atggatccag tgagaaacaa
    11101 taaagcgtgc agtctgttga gactgcacgc tttattgttt aatccaaaat cagtttacta
    11161 ccttagtacg taaaatcaaa cagctcgttt aaacactttt ctagcctcta aagttttgat
    11221 aaatgaataa acaaccatta tttgaatagg gatttggatc atattcctga tgaatctacc
    11281 tagaaaaatt actttgaaat ctgcaccaaa caaaacagac atcagccaag aattgataaa
    11341 aaggctgact gggatcataa tcaataattg ggcagcgatg acggaaggca ggtttatctt
    11401 tttatgatat aaaaataagc cattaataaa tccaccaagg aaagccgtga taagaaaagc
    11461 agcttgaaac gttcctgaac caaatatcgt gaaatttaaa gtgttcgaca acatttcaag
    11521 caaaccagcc cagatcggtc catatagata acccataagc gccatactta aaaatccgaa
    11581 tgtgatttga aaataagcag gaccaatcgc tatcttgtat aagatgacat caagggctaa
    11641 taaaagagca gaggtcgaca acaatcttgc attcatttcg ggcaatctaa aaacgtcttt
    11701 tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt aataataaaa ggataccata
    11761 aaatcaccaa tgtgtcatga cacattggtg atttacatac actggcatct aacaattaaa
    11821 aaaagggtga gaagactgct tttataaact gtaaaaaaga gattgtacga ataaaaaaaa
    11881 tagaggcgga gaatacttat cgtatcatcc gcctttgttt ttttgttata aaaaacgtca
    11941 gattagttat ctgcttacat cttcatccat acgggttcta atgtatttcc gtctagatct
    12001 tggacttcta atgaaaacat ttcttcttca ggcattccca tatctacttc gtggaaagta
    12061 ccaccatttt cttttgcaat ttgagcaaat ttttttactt catccgcgtt ttcaacacta
    12121 aacgaaatca aagcaccact tgttttttta ttatcagcta tttctttatc ttttataaat
    12181 tttttataaa aatcatgaga gagaagcatt atccaaaaag tgtcatccca taccattgaa
    12241 ccagcttctt cagtagaaaa ttcttcattt tttttaaaac ccagcttttc ataaaaagca
    12301 acggaacgat tgacatcagc aacaggaaaa ttaacaaaaa ccatactatt catttgtttc
    12361 gtctcctttc tgttttgttc gtctatcatc tcttatttag gaaacgctgt ca
//
LOCUS       DAHHPA010000078        12309 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.079, whole genome shotgun sequence.
ACCESSION   DAHHPA010000078 DAHHPA010000000
VERSION     DAHHPA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.079"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="NBH55_002101"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="NBH55_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCD1510077.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="NBH55_002102"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="NBH55_002102"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCD1510078.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="NBH55_002103"
     CDS             complement(2169..2906)
                     /locus_tag="NBH55_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1510079.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="NBH55_002104"
     CDS             complement(2997..3524)
                     /locus_tag="NBH55_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510080.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="NBH55_002105"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="NBH55_002105"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCD1510081.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="NBH55_002106"
     CDS             complement(4401..4886)
                     /locus_tag="NBH55_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCD1510082.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="NBH55_002107"
     CDS             complement(5039..5236)
                     /locus_tag="NBH55_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510083.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="NBH55_002108"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="NBH55_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCD1510084.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="NBH55_002109"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="NBH55_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCD1510085.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="NBH55_002110"
     CDS             9211..9423
                     /locus_tag="NBH55_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCD1510086.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="NBH55_002111"
     CDS             9425..11104
                     /locus_tag="NBH55_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD1510087.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="NBH55_002112"
     CDS             complement(11358..11558)
                     /locus_tag="NBH55_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1510088.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="NBH55_002113"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="NBH55_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3589 a         2654 c         2132 g         3934 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaact
//
LOCUS       DAHHPA010000079        12204 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.080, whole genome shotgun sequence.
ACCESSION   DAHHPA010000079 DAHHPA010000000
VERSION     DAHHPA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.080"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(183..1031)
                     /locus_tag="NBH55_002114"
     CDS             complement(183..1031)
                     /locus_tag="NBH55_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1510089.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1030..2097
                     /gene="gnd"
                     /locus_tag="NBH55_002115"
     CDS             1030..2097
                     /gene="gnd"
                     /locus_tag="NBH55_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCD1510090.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2099..3100
                     /locus_tag="NBH55_002116"
     CDS             2099..3100
                     /locus_tag="NBH55_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCD1510091.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3097..3381
                     /locus_tag="NBH55_002117"
     CDS             3097..3381
                     /locus_tag="NBH55_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCD1510092.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3414..4211
                     /locus_tag="NBH55_002118"
     CDS             3414..4211
                     /locus_tag="NBH55_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD1510093.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4225..5058
                     /locus_tag="NBH55_002119"
     CDS             4225..5058
                     /locus_tag="NBH55_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1510094.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5076..5933
                     /locus_tag="NBH55_002120"
     CDS             5076..5933
                     /locus_tag="NBH55_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCD1510095.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5955..6464
                     /locus_tag="NBH55_002121"
     CDS             5955..6464
                     /locus_tag="NBH55_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1510096.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6501..6902
                     /locus_tag="NBH55_002122"
     CDS             6501..6902
                     /locus_tag="NBH55_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510097.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7014..7367
                     /locus_tag="NBH55_002123"
     CDS             7014..7367
                     /locus_tag="NBH55_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510098.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7401..7844
                     /locus_tag="NBH55_002124"
     CDS             7401..7844
                     /locus_tag="NBH55_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCD1510099.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7861..8946
                     /gene="uxuA"
                     /locus_tag="NBH55_002125"
     CDS             7861..8946
                     /gene="uxuA"
                     /locus_tag="NBH55_002125"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCD1510100.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8939..9652
                     /locus_tag="NBH55_002126"
     CDS             8939..9652
                     /locus_tag="NBH55_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCD1510101.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            9981..11282
                     /locus_tag="NBH55_002127"
     CDS             9981..11282
                     /locus_tag="NBH55_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCD1510102.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(11360..11509)
                     /locus_tag="NBH55_002128"
     CDS             complement(11360..11509)
                     /locus_tag="NBH55_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510103.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            11905..12105
                     /locus_tag="NBH55_002129"
     CDS             11905..12105
                     /locus_tag="NBH55_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1510104.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         4060 a         1997 c         2599 g         3548 t
ORIGIN      
        1 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
       61 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      121 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      181 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      241 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      301 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      361 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      421 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      481 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      541 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      601 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      661 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      721 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      781 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      841 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      901 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
      961 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1021 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1081 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1141 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1201 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1261 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1321 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1381 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1441 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1501 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1561 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1621 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1681 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1741 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1801 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1861 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1921 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     1981 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2041 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2101 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2161 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2221 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2281 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2341 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2401 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2461 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2521 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2581 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2641 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2701 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2761 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2821 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2881 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     2941 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3001 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3061 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3121 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3181 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3241 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3301 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3361 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3421 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3481 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3541 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3601 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3661 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3721 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3781 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3841 tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg
     3901 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     3961 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4021 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4081 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4141 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4201 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4261 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4321 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4381 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
     4441 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4501 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4561 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4621 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4681 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4741 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4801 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4861 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4921 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     4981 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5041 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5101 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5161 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5221 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5281 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5341 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5401 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5461 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5521 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5581 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5641 agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5701 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5761 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5821 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5881 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     5941 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6001 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6061 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6121 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6181 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6241 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6301 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6361 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6421 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6481 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6541 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6601 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6661 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6721 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6781 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6841 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6901 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     6961 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7021 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7081 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7141 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7201 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7261 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7321 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7381 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7441 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7501 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7561 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7621 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7681 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7741 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7801 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7861 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7921 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     7981 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8041 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8101 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8161 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8221 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8281 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8341 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8401 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8461 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8521 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8581 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8641 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8701 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8761 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8821 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8881 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     8941 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9001 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9061 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9121 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9181 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9241 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9301 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9361 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9421 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9481 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9541 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9601 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9661 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9721 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9781 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9841 tatgagggtt tccacggctc tataatttct aggactgata gaaaatcatt ctatcggtcc
     9901 cttttgagca tgaaaaaaca aaacatacag aagctccgtt agaattgaat caccacaaca
     9961 caatcaaagg agcgttctgt atgcctgatc ttcatcatat caaactacta cttggaatca
    10021 aagataaaaa cattttcatc acaaatgtgg aatcgaagag cattaagaat atcaagagtc
    10081 ttgttgtttc tgcgacgtta agtaaagaaa ttcgtcgctg tcctttgtgt aaacagatga
    10141 atcatgaagg catgatcgtt aagaacggaa agaaaaaatc gctcattcaa cttaataaat
    10201 gtgcgaacca actgacttat cttgctttag cgaaacaacg ctaccactgt cgtggctgcc
    10261 acacctattt tacggcgaat acctacattg tcgatagaaa ctgctttatc gctaaacaag
    10321 tgcgctataa aattttagaa gagctaactg aaaaacaagc aatgacgaca attgctaagc
    10381 attgcggcgt ttcttggtcg accgtttctc ggacactcgc ttctctgatc cccatgacaa
    10441 aagtaaaaag aaattggtta ccgcgttgtt tattaatgga tgaatttcgt tcactgaaaa
    10501 atcaagtcgg tccctactcc ttttcttgta tggatggcga tactggaaaa ttactggata
    10561 ttcttccttc acggaagaaa aaggctctcg tttcttattt tatgcaattt gaacgacgcg
    10621 cacgattgaa ggtaaaggtc atcgtgacgg acatgaatgt ttcttatgcc tctctgataa
    10681 aggaatgttt tccaaaggca aaactcgttg tggatcgttt tcacattgtc aaacatctca
    10741 ttcgtaaatt tgaggatatt cgcgtacgaa tcatgaaaag ctttgatcga aacgatccaa
    10801 cacaagccaa atattatcga caattaaagg cgttgtctcg gcttctgatc aaacgacagg
    10861 acaagcttgt ttatgacaaa tggaccaaat ggcgcaattt tggttgggcg tatctcaccg
    10921 aaagtgaagt ggtggatcgt ctgctttcga tctctgacga attacgaatc gcctattttt
    10981 actatcaaga aattcttcaa gcgtttcacg acaaagaggc ggacactttc tttaaacttg
    11041 taagaacgat gcccaattcc gttccaaaag aactgcattc tataaaaaaa gcttttataa
    11101 actatgaatc cggcattcgt ttagccctcg aattgcctta ttcaaatgcc aaaatagaaa
    11161 atctgcatac ccacatcaaa gcattgaaac gagtcgctta tggctttcga agctttcgga
    11221 aaatgaagac cagaattttc ttactaaata atctgattac ctatgaatca aaaaacatct
    11281 aaagtagaaa aggaattcta ccttagatgt tcttatctca ctctatcagt cctatttgac
    11341 aaagagccgt ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg
    11401 taacacctgt cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat
    11461 ctgtatccaa ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc
    11521 tttctttaaa tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag
    11581 tataccacca attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata
    11641 aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg
    11701 attcgaagac aatagaagac aatgaatttt atatttcatt gcattatcct tgtgttatta
    11761 gaaaaagcat gatatattaa atgagtaatc tattttgaaa cgtaatttga gcgatatatc
    11821 tacatagata aaactccttt tgcctatcaa atatttattg caacgaaaaa acgtacattg
    11881 atacgtatca ggaggaattt atttatgaat aacggtacag taaaatggtt caacgcagac
    11941 aaaggttttg gctttatcac tggtgaagat ggaaatgatg tatttgcaca tttttcagct
    12001 atccaaggtg acggcttcaa aactttagaa gaaggccaag cagtgtctta cgatgttgaa
    12061 gatggtcaac gtggccctca agcaacaaac attgttaaag cttaataaaa attgactttt
    12121 aaccgtcttt tcgaaagaaa agacggtttt tttgtacgaa aaaattcatt ggtcgttgaa
    12181 tgggctgaat agtagtgtat aaaa
//
LOCUS       DAHHPA010000080        12138 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.081, whole genome shotgun sequence.
ACCESSION   DAHHPA010000080 DAHHPA010000000
VERSION     DAHHPA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.081"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..132)
                     /locus_tag="NBH55_002130"
     CDS             complement(<1..132)
                     /locus_tag="NBH55_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCD1510105.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="NBH55_002131"
     CDS             542..841
                     /locus_tag="NBH55_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1510106.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="NBH55_002132"
     CDS             914..2275
                     /locus_tag="NBH55_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCD1510107.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="NBH55_002133"
     CDS             2297..2557
                     /locus_tag="NBH55_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510108.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="NBH55_002134"
     CDS             2571..3323
                     /locus_tag="NBH55_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCD1510109.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="NBH55_002135"
     CDS             complement(3417..4022)
                     /locus_tag="NBH55_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1510110.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4075..5049)
                     /locus_tag="NBH55_002136"
     CDS             complement(4075..5049)
                     /locus_tag="NBH55_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCD1510111.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5427..6146)
                     /locus_tag="NBH55_002137"
     CDS             complement(5427..6146)
                     /locus_tag="NBH55_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1510112.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(6273..7544)
                     /gene="celB"
                     /locus_tag="NBH55_002138"
     CDS             complement(6273..7544)
                     /gene="celB"
                     /locus_tag="NBH55_002138"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCD1510113.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7697..9094
                     /locus_tag="NBH55_002139"
     CDS             7697..9094
                     /locus_tag="NBH55_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD1510114.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9175..9615
                     /locus_tag="NBH55_002140"
     CDS             9175..9615
                     /locus_tag="NBH55_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD1510115.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9763..10089
                     /locus_tag="NBH55_002141"
     CDS             9763..10089
                     /locus_tag="NBH55_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCD1510116.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10090..10839
                     /locus_tag="NBH55_002142"
     CDS             10090..10839
                     /locus_tag="NBH55_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510117.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10919..11374)
                     /locus_tag="NBH55_002143"
     CDS             complement(10919..11374)
                     /locus_tag="NBH55_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCD1510118.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11801..12076)
                     /locus_tag="NBH55_002144"
     CDS             complement(11801..12076)
                     /locus_tag="NBH55_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510119.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3856 a         2108 c         2455 g         3719 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     4081 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     4141 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     4201 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     4261 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     4321 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     4381 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     4441 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     4501 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     4561 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     4621 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     4681 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     4741 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     4801 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     4861 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     4921 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     4981 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     5041 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     5101 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     5161 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta
     5221 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     5281 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     5341 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     5401 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     5461 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     5521 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     5581 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     5641 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     5701 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     5761 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     5821 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     5881 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     5941 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     6001 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     6061 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     6121 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     6181 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     6241 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     6301 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     6361 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     6421 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     6481 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     6541 atcggtagac cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat
     6601 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     6661 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     6721 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     6781 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     6841 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     6901 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     6961 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     7021 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     7081 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     7141 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     7201 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     7261 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     7321 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     7381 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     7441 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     7501 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     7561 tttatttgca atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt
     7621 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     7681 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     7741 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     7801 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     7861 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     7921 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     7981 tcaaaaagct gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga
     8041 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     8101 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     8161 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     8221 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     8281 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     8341 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     8401 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     8461 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     8521 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acttgctagg
     8581 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     8641 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     8701 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     8761 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     8821 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     8881 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     8941 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     9001 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     9061 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     9121 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     9181 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     9241 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     9301 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     9361 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     9421 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     9481 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     9541 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     9601 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     9661 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     9721 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     9781 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     9841 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     9901 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     9961 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
    10021 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
    10081 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
    10141 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
    10201 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
    10261 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
    10321 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
    10381 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
    10441 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
    10501 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
    10561 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
    10621 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
    10681 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
    10741 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
    10801 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
    10861 tattctattt atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt
    10921 atatataata atttaaaaat tcatcatcta aatccgtaga attttctaga ttgcttaccc
    10981 atgtaaaatc gttatagaca gatttcagtt gacttctgcg ccattgtaca gggataaaaa
    11041 gacgcatgct atcttcattg aatccgacta ataatttctt tccgtcagta atgatgggac
    11101 gttgtaacaa agtaaaatgc gagacgatca gatcgacaag ctctgaggtc gtacattggt
    11161 cgatttggtc tttcaaattt ttataatcaa tcgagcgttt agctaataga tcgtcaaatc
    11221 cgttttctgt taatgacaat aaataaaata tctctttcct tgttaaaggc tgtttaggtg
    11281 tgaaaattcg ttctctgtaa ggcatttgat ggtctttcag ccagtttgcc atcagatggt
    11341 aactatgtgt gcgagaagaa tagatcgtaa gcataaaaaa actccttaaa tagtccatta
    11401 atttgttaga tctgttgaga taaataatag gtaaggagct gtgcataagc attgatcaaa
    11461 acaacccaaa catcattaga aataactttc taaagtctta aacttgcgac gagaaacaac
    11521 tagactaata gaaaaaatgt cttagccatt tggcttcctg tttgtttttt tgcttatgct
    11581 gcagctcctc gggaaagggt tctaacaatt tgaagaaggt tgatgattga tctagtttaa
    11641 attgtgtgaa atttgaaatc tggtgttttt actagatgaa tcacctaaca actatgatta
    11701 taacttatag aacttctgtt ttgcattcgg ttttgttata gttgttataa agttttattt
    11761 tattacagaa catttttaat taataaagaa cagttagaag ttaaaattgc tgattcacat
    11821 agagtttatg acttcttgga aattcttttt caatcagttt tttcatctgt ttatgaaatt
    11881 cttttttttc agtaaacata aaggtttggg tatcgtaacc ttcttcatgt aggaaataga
    11941 ttattccttt ttctgtgttt tgcgtataag cttttatact cctgtctttt ttgaaagaac
    12001 agatcgtata gccatctatt tggtctggta gtcgtttgat tttcttcaaa atgacttttt
    12061 tatcctcaag tttcattttt ttcacctgac tttatttttt tcatatggaa atcatagcgc
    12121 ctttggttat atgaaaac
//
LOCUS       DAHHPA010000081        12107 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.082, whole genome shotgun sequence.
ACCESSION   DAHHPA010000081 DAHHPA010000000
VERSION     DAHHPA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.082"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            34..402
                     /locus_tag="NBH55_002145"
     CDS             34..402
                     /locus_tag="NBH55_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCD1510120.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            572..1003
                     /gene="mraZ"
                     /locus_tag="NBH55_002146"
     CDS             572..1003
                     /gene="mraZ"
                     /locus_tag="NBH55_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCD1510121.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1024..1983
                     /gene="rsmH"
                     /locus_tag="NBH55_002147"
     CDS             1024..1983
                     /gene="rsmH"
                     /locus_tag="NBH55_002147"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCD1510122.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1988..2389
                     /gene="ftsL"
                     /locus_tag="NBH55_002148"
     CDS             1988..2389
                     /gene="ftsL"
                     /locus_tag="NBH55_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCD1510123.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2390..4582
                     /locus_tag="NBH55_002149"
     CDS             2390..4582
                     /locus_tag="NBH55_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD1510124.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4608..5570
                     /locus_tag="NBH55_002150"
     CDS             4608..5570
                     /locus_tag="NBH55_002150"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCD1510125.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5584..6945
                     /locus_tag="NBH55_002151"
     CDS             5584..6945
                     /locus_tag="NBH55_002151"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCD1510126.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6971..8059
                     /gene="murG"
                     /locus_tag="NBH55_002152"
     CDS             6971..8059
                     /gene="murG"
                     /locus_tag="NBH55_002152"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCD1510127.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8074..9294
                     /locus_tag="NBH55_002153"
     CDS             8074..9294
                     /locus_tag="NBH55_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCD1510128.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9450..10775
                     /gene="ftsA"
                     /locus_tag="NBH55_002154"
     CDS             9450..10775
                     /gene="ftsA"
                     /locus_tag="NBH55_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCD1510129.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIIDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10798..12039
                     /gene="ftsZ"
                     /locus_tag="NBH55_002155"
     CDS             10798..12039
                     /gene="ftsZ"
                     /locus_tag="NBH55_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCD1510130.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
BASE COUNT         4217 a         2054 c         2639 g         3197 t
ORIGIN      
        1 cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg
       61 ttttggtata tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt
      121 tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc
      181 ggtatgcatg cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt
      241 tctgttttgc taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt
      301 atttatccgc gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg
      361 tacgctgtct tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt
      421 aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa
      481 aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa
      541 ttttagttag tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat
      601 tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt
      661 tgttctgaca cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa
      721 tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg
      781 attcttctat tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc
      841 cagcacgtta agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa
      901 ccgaattgaa atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa
      961 ctttgacgaa attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga
     1021 ctaatgactg aaacatttca acactataca gtcatgttga aagaaacggt agatggattg
     1081 aacatcaaac cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa
     1141 tatttattaa gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa
     1201 gcaatcgatc acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt
     1261 atcaaagcga acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac
     1321 ggcattttgt atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt
     1381 agttatcatc aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct
     1441 tatcacgtaa ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc
     1501 gaggagaaat tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca
     1561 atccaaacaa ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga
     1621 agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac
     1681 gaactcggtg ccgaagaggc ctctttagaa caggcaatcc ggttattgaa agtcaatgga
     1741 agaatcagcg taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa
     1801 gaatatagta cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa
     1861 ccagaactga aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa
     1921 aacaatcgat cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag
     1981 tagaatgatg gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt
     2041 ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca
     2101 atcaccggca aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt
     2161 ggcgattgct attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg
     2221 agctgaaaaa gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac
     2281 gagtttagaa caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga
     2341 agaaaaagga ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa
     2401 aaataaaatt cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa
     2461 agtaggtatc attttatttg ctacgagtat tggattgttc tttttatttg ccgtccgttt
     2521 ctcgtatatc gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca
     2581 gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg
     2641 tgtggcgctc gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata
     2701 tacctctggg gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat
     2761 cctgcataag aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc
     2821 aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac
     2881 caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga
     2941 ccatcaggct cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt
     3001 tccagacaaa gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga
     3061 tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct
     3121 gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt
     3181 agacagcaga ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca
     3241 gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg
     3301 acaacgacct actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg
     3361 gcgtaatttc ttggtacaag atagttatga accaggatca accatgaaag ttttcactac
     3421 tgcagctgct atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat
     3481 ccaagtagaa gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat
     3541 gcgtcaagct ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg
     3601 cggacgatgg tataactatt tacaaaaact gggatttgga caaagtactc attctggatt
     3661 agatgatgaa gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc
     3721 ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc
     3781 gaataacggt acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg
     3841 agaagagcga acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga
     3901 aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg
     3961 tgtatatagt gtacctggat acaatgtatc ggcaaaaaac ggaacagccc aaatcgcttc
     4021 tgatacaggg ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt
     4081 tccttctgaa gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga
     4141 tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga
     4201 aactgaagaa gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg
     4261 taatcttgaa gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt
     4321 gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc
     4381 tggagaaaaa ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg
     4441 gtcaaaagca gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg
     4501 agatggttat tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact
     4561 tagttttact ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac
     4621 aagcattgat tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta
     4681 ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc
     4741 acaatagcaa agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt
     4801 tgacaggaat ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc
     4861 tttttgtatt agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca
     4921 agaaaagaaa catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa
     4981 tcatctttta tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac
     5041 tagatctacc tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt
     5101 caaatgcagt aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct
     5161 cttttgcagc ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt
     5221 gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga
     5281 tttttatggg agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa
     5341 tgctaaacca agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa
     5401 gtgtgatgct tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc
     5461 caattcatca tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt
     5521 ggttgatcag cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg
     5581 gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa
     5641 agcggagtaa gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat
     5701 ggaaaacctt ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta
     5761 ataacaggta gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat
     5821 ccagggattc cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta
     5881 attacggaag ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga
     5941 acgaacggaa aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat
     6001 caaggaattg cacgtttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa
     6061 gcaaaatcag aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca
     6121 gatttccgcc ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat
     6181 ggtacaagaa aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa
     6241 gattatctga tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa
     6301 gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat
     6361 tactcaatat actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt
     6421 aagcataatg tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca
     6481 aatgaagcta tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat
     6541 gtaggagaaa tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg
     6601 actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta
     6661 gacagaggaa atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc
     6721 gtatttggcc aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca
     6781 atagtaacag ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat
     6841 ggtgatgtag ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa
     6901 actagaggaa acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga
     6961 gcgataaaaa atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc
     7021 tcttgccttt gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg
     7081 agcaaaacga ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt
     7141 ggagattcaa ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt
     7201 tcttaagagt atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat
     7261 cggtactgga ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc
     7321 aacagttatc catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata
     7381 tgtggatcgg atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc
     7441 agttcttgta ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt
     7501 gaaaacattt gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg
     7561 agcacttaaa atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta
     7621 ccaagttttg tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc
     7681 tgaaatacaa aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc
     7741 cagtgatctg ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg
     7801 gttaccggca gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc
     7861 aatgagtctt gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga
     7921 atcactaagt cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg
     7981 ccgagcttct aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa
     8041 acaaataatc aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa
     8101 ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa
     8161 tatctggcag aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat
     8221 acaccccttt cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc
     8281 cagtccgaga aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca
     8341 tataacggtt cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta
     8401 tttcgtcggt tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat
     8461 tatgtttctc cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac
     8521 agccaagaaa tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat
     8581 tttcatcgat ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca
     8641 aatatacatt tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt
     8701 gctttgatcg caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat
     8761 gaaaaagtag caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca
     8821 aaaatcaaag aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt
     8881 tctgaaatca agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat
     8941 gacggcaatc aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg
     9001 caagtagcca aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg
     9061 catccttacg gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa
     9121 agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca
     9181 caaacacctg gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca
     9241 agtcaaatag aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac
     9301 aactttaaaa agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc
     9361 agtgaatata tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat
     9421 ggaatagtaa gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg
     9481 atattggaac gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata
     9541 ttattggagt aggaaacgca aaatcagaag ggattaaccg aggcattatc attgatatcg
     9601 ataaaacggt ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc
     9661 agatcaaagg cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg
     9721 gaatgattgc agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg
     9781 cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac
     9841 aagattttac tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa
     9901 gattagaaat gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta
     9961 aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga
    10021 ctgaatcaat tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg
    10081 gcggccaaac aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc
    10141 aagaaggtgg cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca
    10201 atgcagaagc gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg
    10261 aagaatttcc agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact
    10321 tgtcagaaat tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg
    10381 accaaatcga agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc
    10441 ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc
    10501 caaatcacat ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt
    10561 cagcaaactt gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag
    10621 cagctcgtca aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac
    10681 ctgaagaaac aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata
    10741 aaatcaaggg tttctttaca aatatttttg attaattcat cctgggagga ataagatatg
    10801 gaattctcaa ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt
    10861 ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc
    10921 attacagcca acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa
    10981 ttaggcccca aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa
    11041 gcggcagaag aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc
    11101 atcactgcag gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc
    11161 gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga
    11221 ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca
    11281 ttattaatta tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt
    11341 gaagctttcc gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg
    11401 atcacagcgc caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac
    11461 caaggtacag cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca
    11521 acgaaaaaag caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta
    11581 cttcttaaca tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat
    11641 atcgtagcaa atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa
    11701 gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag
    11761 gaacgcaagt ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact
    11821 gtattagata tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat
    11881 tgggatatca gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat
    11941 atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat
    12001 gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat
    12061 tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat tcgtgtg
//
LOCUS       DAHHPA010000082        11708 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.083, whole genome shotgun sequence.
ACCESSION   DAHHPA010000082 DAHHPA010000000
VERSION     DAHHPA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.083"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(11..1342)
                     /locus_tag="NBH55_002156"
     CDS             complement(11..1342)
                     /locus_tag="NBH55_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1510131.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1491..2204)
                     /locus_tag="NBH55_002157"
     CDS             complement(1491..2204)
                     /locus_tag="NBH55_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1510132.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            2379..3803
                     /locus_tag="NBH55_002158"
     CDS             2379..3803
                     /locus_tag="NBH55_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD1510133.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            4102..4809
                     /locus_tag="NBH55_002159"
     CDS             4102..4809
                     /locus_tag="NBH55_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCD1510134.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4796..5119
                     /locus_tag="NBH55_002160"
     CDS             4796..5119
                     /locus_tag="NBH55_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCD1510135.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(5208..6245)
                     /locus_tag="NBH55_002161"
     CDS             complement(5208..6245)
                     /locus_tag="NBH55_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD1510136.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(6566..7549)
                     /locus_tag="NBH55_002162"
     CDS             complement(6566..7549)
                     /locus_tag="NBH55_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCD1510137.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(7964..8734)
                     /locus_tag="NBH55_002163"
     CDS             complement(7964..8734)
                     /locus_tag="NBH55_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1510138.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8727..9662)
                     /locus_tag="NBH55_002164"
     CDS             complement(8727..9662)
                     /locus_tag="NBH55_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1510139.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(9978..11627)
                     /locus_tag="NBH55_002165"
     CDS             complement(9978..11627)
                     /locus_tag="NBH55_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCD1510140.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
BASE COUNT         3547 a         2396 c         2040 g         3725 t
ORIGIN      
        1 tcatagtaaa ttatgtttca ttttcactta atttagttgt cacgtgttcg tcaattcgta
       61 aaaacggtaa atatataaac acacttgcgg ctataattaa tatttgtaaa acagctgctc
      121 gccagtctcc ggctgttgct aaaaatcccg atataactgg tggcgtagtc caaggaattt
      181 gcactactac atgattaatt aatcccacag cagttgcaaa atatgctact gtcaaagaaa
      241 aaatcggtgc aagcacaaag ggaataatca gactgatatt aaatacaata ggcaatccaa
      301 aaataatagg ttcgcttata ttgaatatag cgggagttac cgataattta gcaaagtcct
      361 tataatcttt tctacgactg aagataaata ttgcaatcaa taaagcaatc gtatttcccg
      421 atccacccat taccgcaaag gtatctttaa aggccatatt gataatatgt ggaatttgct
      481 ctttgtttgc ataagctgcc atattttctt gcatattctg aatcaaaaac ggatctaaca
      541 atgaacctga tattactgct tgatggatac caaatccaaa aaatagatta gcaatagatg
      601 taattaacaa aaatcctggt aaaccagttg taattttact taatggctct gtaactatcg
      661 tggtaattag cttatttata tccatattaa acaattgaat gattataaat gacaataaag
      721 caaacgaggt aatcgtaata atgattggga ttaatacatt aaaagacttt aaaacagcgg
      781 gaggaatatt tccggatagc tgaatctgta gtttcttatt attagaaagt ttaataaata
      841 attctgtagc cgctaatcca acaaatatac ctacaaacat tcctgaagct cctgtatagg
      901 atataggtat cacacccgtc acttcaacag ctttatccgc attttcagga gtaaacatca
      961 tctgcatagg tgttaataca acaaatgtcg aaatagcaac taataccgct gcaatatgat
     1021 ttgtataatt tctgcttgca cacatatgat aagatactgc tgctacaact aataatgtag
     1081 ttatgcttaa tgtaccattg gcaattgaag taccaatttg ttgtaacgtg gtcaatgtac
     1141 tagtatcgac aatttccccc atgaatcctg ttggttctaa gatcactgaa ttaataagtg
     1201 tcacaaatcc tgctaacatc ataaatggca taaatgtagc aaacgcatct cgcaaagttc
     1261 ttaaatggat ctgattcccc aacttagtcg caactatacc aaatttttca aatacaatgt
     1321 ccttcctgct acttttgccc atattttttc tcctttatgc ttattgtaat cgattacttt
     1381 ttccacccaa ataatacatc acttatcaaa aaaagtaaat acttttaatt aaatatatat
     1441 atctttttta gtataaaaaa ataagctctg aaaaaataat tttcccgagc ttatttttca
     1501 acaaaaacag cttcaaaaac aaagtcttga tataaatgtc tcgataccga atactcgaaa
     1561 ggtttaccag acgatagagc aacaactttg tctatctcaa tcataaattc acctggatct
     1621 atttttaata tttttgcgtc aaattcattc gcagtatctc ctttgatacg tatgtgcgaa
     1681 gtctttattt ccagacccag ttcatttgtg atataagcat atacagattt ctttaaatgt
     1741 tttggttcca agcctggtat gactgctaat ggcatgaatg tttgctcaat cgagtatggt
     1801 tgacaatcaa taaaacggct tcgtacaata ttatatacat agtcttttac actgatactt
     1861 aatttatcag ctactttttt cgatggaagt tcaattgaga aagtatggat ttctgaatct
     1921 acgtgttctt caccaaccac gctaaacaaa ccttcgtttg gaccaagaac tcgtgagtta
     1981 tcaattggtg ttctgacgac ttcagtccca tacccacttc tacttcgcaa aactccttcg
     2041 gaaacaagta agtctacgcc tttctttact gttaacttgc ttgtatgata aataactgcc
     2101 aattcatctc ctgtaggaag tttctcacca attttatact ctccatttac aatttttttc
     2161 tttatatcta agtaaatttc catatacttc gcaatttttt tcaatggtga ccctccaatc
     2221 aaaaacatat cttcatctat actttaacat aaataaaccg ataaaatcca catcaaaatt
     2281 atacacacaa taaaaaagat atatatattt aaacaaaact atttacttta agttgtaagc
     2341 ggtgttatca ttaagtggaa acaggaggta aaaaaattat gacaactatg aaaaagctaa
     2401 cttttccaga tgatttttgg tggggttccg cttggtcagc tgagcaagca gaaggaaaag
     2461 gagaaacagg aaaagcagag actatctggg atcgctggtt taaagaacaa ccttaccggt
     2521 tttatcaacg aatcgattcc tctattacaa ccgatcacat ccatcaatat aaagaagata
     2581 tagaactaat gaaaaaaact gggcataact cctttcgcgt ttctatttct tgggcacgaa
     2641 tgtttccaaa tggcggtacc ggaaaagtaa atcccaaagc catttctttc tatcgaaaat
     2701 tattttccga aatgaataat aacggaatca aagtatttgc caacttatat cattttgaca
     2761 tgcctgctac tcttcaagat aaaggaggat gggaatcacg tgaagtagtt gatgcttatg
     2821 ttcattacgc tgaaacttgt tttaaagaat ttggtgactt agtttgtcat tggtttacct
     2881 tcaatgaacc acttgggcct attttaggta cttatcttga agattttcat tatccaaacc
     2941 tcattgactt taaacgcgga gcgcaagcag cattcttcac tatacttgca cacgcaaaag
     3001 caatacaagt atttaaagaa ctagctcttc ctagtaaaat aggcgttatt ctaaatttga
     3061 gtcctacttt tcctcgtagt caaaatcctg ctgatgtgaa agcagcagaa atcgctgatt
     3121 tattttatac gcggagcttt ttagatccta tggttaaagg gaatttttct aaaaagctta
     3181 tcctgttatt acaagaatat aatcagcttc ctgaagatta tacgaaagaa gatcttgtat
     3241 gtatctacga aaataccgct caaattcttg gactaaacta ttacgagcca cgtcgcgtca
     3301 aatctcgttc ttctatggtg gatacatcta gtccattttt accagagtgg tttttcgaac
     3361 cacatatcat gcctggacga cgaatgaacg aataccgggg atgggaaatt tacgaaaaag
     3421 gaatttatga tttgtgcatg gatatcaaga accattatgg aaacatcgaa gcatttatct
     3481 cggagaacgg aatgggcgtt gcggatgaag aacgtttcat ggatgataca gggcaaataa
     3541 ttgatgatta tcggattgag tatatcaaag atcatcttgc ttacttatgg aaggcaataa
     3601 atgaaggatg taacattaag gggtatcatc tttggacatt tattgattgc tggtcatgga
     3661 tcaacgccta taaaaaccga tatgggctgg tttcactcga ccttactact caaaaaagaa
     3721 caattaaaaa aagtggcgaa ttttacaagc aattaagtga tacaaatgga tttagatacg
     3781 atgtagactt gttagttgaa taatattcta aaattccact tgaagtgaac acattagaaa
     3841 acaagaataa tgagaaatac caataacaaa tactttttct tcatacttca tttatacttg
     3901 tcagtataag agactgtgac aaatgtagtg atcctcagaa gttaggtctg gaaatctaac
     3961 ttgagaagat ttttttaaac tacgtccagt cttttttatt tgtttaaaat tgaagttgac
     4021 aagtcttcat taaaaattta gaatattcac aagtttgacg acaattatga aaatttatgt
     4081 cgtgctaaag gagttttgtt attggatgaa aagttagata taaaaactgc gatcaaagac
     4141 acgttaccta ccgtttttgg ttatatcggt attggacttg catttggtat cgttgggaaa
     4201 gctgccgggt ttcatccgct ggtcgtcacg ttgatgtccc tgctgatcta tgctggttct
     4261 gcccaattta tcacggtcag catgcttgct agccatagtc cactgctctc catcgttctc
     4321 tcgacctttc tagtcaattc ccgaatgatt ctgatgagta tgacgattgc tccttatttc
     4381 aagaaaaatc ggctgcttca aaatctgctg attggcacat tgctgacaga tgaaagtttc
     4441 gcactgggaa tgaataaatt gaactatact ggacaaaagt taaactttcg ttggatgaat
     4501 acagctaatt ggatttctta tttgacgtgg gtgggttctt ctcttgttgg cgcactttta
     4561 gggaatttta ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt
     4621 atcgggttac tttatcttca agtcatttct gatagaaaca cctctaaacg attacagttg
     4681 atcctgattg gtctcacttt gattctggta tatgtcggac tgatttttat cccaagtaac
     4741 ttagtgattt tagttgtaac actgattggg tgcggattgg gggtgtggat caaacatgcc
     4801 ttcttttgag tttacattgc tgactatttt aggttgtact ttagcaactt ggctttctag
     4861 gatcctacca tttgtcttac tgaaaaaatt cgatttgccg caaccgttgc ttgagtattt
     4921 aagttttgtc ccgatcgtca ttatgtctgc tttatggttc agcagtctat ttacccaaaa
     4981 tatcggacat ctgccgcaga tcaatctcga aaatgctttg gcttcagttc ctacactttt
     5041 agctgctatt ctttcaaaaa gcttattggt gatcgtcttg gtaggaattc tttccctttc
     5101 gttgattcgc ttattataaa taaaaaaaca agagcttatg gcaaattact gtcatgcgct
     5161 cttgttttta tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta tccgttgaac
     5221 ggaccactaa caacgacttt tcctaatata tgtcctgttt ctacgtcttt ggttgcttgt
     5281 ttcaagttct tcgcattcat cccatctgaa tagaccttgc tcaatgtact gcggataatc
     5341 ccttcatttg ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc gatttcatag
     5401 ttatagtctg ttttagcgaa catatactcc cagtcgaagc tgatagattg gtttttccaa
     5461 acacctatat ctaacggctc tttgatccct acgatcgttc ccacatgtcc aaatggtcgg
     5521 acaaggtgtt tgatttgatc aaagtagcgg gtaatatcga ataatacagc gacatactct
     5581 acttgatcga ttcctaattc acgaaaactt ttacttaagt cgtgatgata atcgattgga
     5641 tagtctacac ctgttttttt cagccattca aaattcttcg gacttgcagt cgcgtagact
     5701 tctaggccag cccaatgcgc tagttgattc aaaatcgaac cgacgccccc agctccgttg
     5761 atcactaaga tttttttccc gcggttggca ttttcttctg gagtcagacc aaatttttca
     5821 aataacaatt cataagcggt caatgaggtc aacggcaagg ctgcggcttc ttcatctgaa
     5881 agattttttg gtgcaagagc gacaatacgt tcgtcaacca gttggtattc ttgattgctt
     5941 cctgctcttg tcgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact
     6001 tcatcaccta cagcaacaac tttcccgact ccatcaaacc ctaagatacg taaagcattt
     6061 cttatcccgt tatcttggcg aagtttcgta tcgacaggat taacagatac tgcgttgact
     6121 tttaccagaa gatcccgtga actgggtaca ggtatctgtt ctttttcatc aataaagctt
     6181 tttggatcat cgatcgacaa cccttcataa aaaccgacta ccttcattaa ttccttttct
     6241 gacatacgaa ttcctcctta gcttcatcaa gttggagcat ccatacagtt ttcatttcaa
     6301 tatatatagt atgctatttg ttccactttt attcatcata attattctct tacttaaagt
     6361 aaactatttg cacttttttg gttaaaaaag aggttgcgac aagacttttg tcacaacctc
     6421 ttttaaacgg tgttcaatca actgaccctc ggtattcgct caaaaaacga caaaatatga
     6481 ggagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacgacctc
     6541 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
     6601 caaaggtgtc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
     6661 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
     6721 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
     6781 gtccgatcag ttctttttct gtatgttccc tcggttcttt ttcaaatgca taaggattct
     6841 tgaaaatccc ccggccaatc atgatgccat ctacgccata tttttcggcc aattcttctc
     6901 ctgtctggcg atctgggata tctccgttga tcgtgatcag tgtttgcgga gcgatttggt
     6961 cacgtaaagc aatgatttta gggatgactt cccaatgtgc atctacttta ctcatctctt
     7021 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
     7081 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
     7141 cgccagcttt agccgcttca atcaattgtg ccgctacttc tgggcgaagg atcaagccac
     7201 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
     7261 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
     7321 aaatatgggc aaccatcggc tgttcatctt ctgtaaaggt caatcttcct cgtacactgt
     7381 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     7441 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     7501 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     7561 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     7621 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaag
     7681 actgtgacaa aaatcttatc acaattcctt ttccggaata aatggtgttc aaaagcttgc
     7741 atctgcggta ataagtccaa ccaaacgaaa aataggagga gctatttttg tttggctgtt
     7801 cttattactt gatgcagaac agctttttca caacctctcc actaaacggt gttcaatcag
     7861 ctgaccctcg gtattagctc aacaaacgac aaaatatgag gaactatttt cccgtttgtt
     7921 tctctaatac tcaggtcaaa acgctgattt cacaacctct tttctaactt ctcaatcctc
     7981 ttcctttatt gatcaaatac caagtaacgg cgtacaaaac aacgatgaat agcacaagta
     8041 cgatcatcga tgttgctact ggcacatcga ttgttccaag gaatccgtaa cggaaaccgg
     8101 agatcatata aacgatcgga ttgactttcg aaacagcttg ccaaaatggc ggcaacatag
     8161 agatggcata gaaaacgcca cctagataag ttaacggctg caagacaaat gtaggcacga
     8221 tcgatacatc gtcataagac tgagcaaaaa tcccgttgag cagtcctgct aacgaaaaca
     8281 agattgccgt catcaataat gtaatgatga ccatgaacca tgaatacaca tgaagtggta
     8341 caaagaacaa ggaaatgatc gttacgagag agccgacgag aatactgcga cccactccgc
     8401 caatcacaaa gccccaaatg atcacatgcg taggaacagg cgctaccaag atttcttcga
     8461 tgtttttctg gaatttttga gagaagaagg acgaagaaac gttggcatac gaactagtga
     8521 tagcagacat cataatcaaa ccaggcacga tgaattccat atatgaaaaa ccacccatgt
     8581 caccgatccg accaccgatc atttttccga aaataacaaa gtacaaggat gtcgtgatca
     8641 caggcggaac cagtgtttgg acccagatac gcaaatagcg gttcgtttcc ttgatagcta
     8701 aactttttaa tgcagtaaaa taaagattaa acatttttct cctccgtatg attattttct
     8761 tctgtgattt tcaaaaacag ttcttctaaa cgattcgact tattcctcat agaaagaact
     8821 ttgattcctt gtcttgtcag ctgatcgaag attccgttca ccccttgatt tcgttcaact
     8881 tcgacagcga ttgtctgttc atcttcaaat gtacaagcgt atccttcaat cacaggtttg
     8941 gtctcatatg gcgctaaatc aaagataaat gtttcaaatt gcagtttgga tagaagctct
     9001 ttcatactag tattctcgat cagttcacct gattggatga tcccgatgtt gcgacaaagc
     9061 atttcagctt cttctaaata atgcgtcgtc aaaatgatcg ttgtaccatt ttcgttcaat
     9121 tctcggagaa actcccacat ctcgcgtctc aattcaatat cgactcctgc tgttggttca
     9181 tccaagatca gcaaacgtgg ttcatgcatc aatgccctag caatcatcag tcttcgcttc
     9241 attcccccgg aaagcatacg tgcacggaca tttctctttt cccaaagatt ggactgcttc
     9301 aaatattttt cgctgcgttt catcgcttct ttgcgagaga caccgtagta gcctgcttga
     9361 ttcacgacga tttgctggac cgtttcaaag ggattgaaat tgaactcttg cgggactaat
     9421 ccaatctgtt gctttgctcg gactagatcc gtatccagat cataaccgaa gacattcact
     9481 tttccagatg ttttattgac taaagaagta atgattccga tcgttgtaga cttcccagct
     9541 ccatttggtc ctaatagcgc atagaaatct ccttctttca cggaaagatc gattcctcgc
     9601 aatgcttcga ccccacttcc ataaaccttt ttcaaattat ttatttcaag cgcatttgcc
     9661 atttatttcc tatctcctta tagacatgat tctttattgt atcatggaat acagcagatg
     9721 aaaaaacaac ttggttccag cgaaaaatca ggttgctggg ctcaggaaaa tctcctaatg
     9781 aaagacatca atcggacgaa gtcagacaac cttttcagaa aaaacaaatc gaagataaag
     9841 gattactatt catatgtgca acaaaaggag cgtgagacaa aagtaaagaa cacttttgtc
     9901 tcacgctcta aacacgtata aacggcggaa gcaaaagcca agatttttga tccaataaat
     9961 actttcgatc acttcgccta tcccaataca tgccactgcc gcttttcatc aaataacgcg
    10021 gcttccgttt ttgcggtttc tactaaagtc tctgggtaat ccaaaacttt cagcaaggca
    10081 atcgcatttc gggtccttga aggtccttct tttaatttga aatcaaaact gattcctttt
    10141 ccttctgtta cttgttcttc aaagtggaca ttctcacaat aattctttaa aatctctgtc
    10201 aattcgatat catgtgtggc gacaaatgct aaagatggat actccgcaag ccaccgaacg
    10261 actgaagcgg aagaagcaat ccgttcgatc gtatttgtcc cttttaaaat ctcatctacg
    10321 aagcagtagc agcgttcttt ggaagccact tggtccaaca aacgtttgat cgatttgatt
    10381 tctgaaacaa aataactgtc gccttcaaat aaatcatctt cgattgccat ggaagtcaac
    10441 acatgtccag gttgcagggt aaactgttct gcaatcgctg tttggatcgt ctgtgctaga
    10501 atacagctga tggctatact tttgacatac gtagattttc ctgatgcatt ggaacccgtg
    10561 actaatgtat tcttctgcca gttgaccgga ttcactaccg cttcttttaa caatggatga
    10621 tagatatctt tcgctgtcac accgccctca gtaaactctg gtcggcaagt gatgggcata
    10681 taagtacggt aattcaatat tgcagcggct acttctaatt ctcccagcaa ttcccacagc
    10741 ttgatggctt cagttgaatg cttttctaat ttagtgagaa cgaaatgata ggagataaat
    10801 ggcagcatga acatgatgtt gacatattca aaaatcaact ctgcttcact cccactcttc
    10861 atacgaaatg aaaaacctaa tttagggatg tctcttaatg gtttagtttg ctgtttcagc
    10921 tcttcttgaa gtggtgtcgg cagttttgct agctgattgg cggctgaaat cgttgaaacc
    10981 aaataactca tgctattcag ctctgtttct agtgtttgtt tcttcactag ataatagacc
    11041 gtattgaata ctaacccacc gattgccaaa tagattccta gtacttgtcc taaaagaagc
    11101 aaacccaaac ccaccagcgg aagcaagcca agaaaaataa ataaacctac gcgtccaatc
    11161 tttttacttt ttccatccgc tagatattgt tttgcaaaat tatgatcttt ttttcctaaa
    11221 cgagcgaatt gatattggat tttttctctc agttgaggat tttcctgata aaaagcaatc
    11281 agtttttcta actgctgatc ttcaccaaaa tcaaagctgc gtaaacgttg atacaacgct
    11341 tctgaaccta cactagaata ggtactgttg attccttcga atacttcgaa catatccagg
    11401 tcataccaag taagatcatc gatttcgcta tcccatgaac ggaacttctt ctccgtgagc
    11461 catgcttcct tcaagctttc ttctttatca aatctcggtt gataaggaat tttttcccat
    11521 ttgctacgaa ctgtttcctt taatttcaaa cgtgaaaaaa tgtctaaagc aactaataaa
    11581 ataacagctg caataataat taaaacgata aattctcttt gacccatatc cctctccgtt
    11641 cctttatttg ttagctttag tatagcactg ttgcgctatt tgaaaagagg gtaaaaaaac
    11701 acctttca
//
LOCUS       DAHHPA010000083        11514 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.084, whole genome shotgun sequence.
ACCESSION   DAHHPA010000083 DAHHPA010000000
VERSION     DAHHPA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.084"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(79..2154)
                     /locus_tag="NBH55_002166"
     CDS             complement(79..2154)
                     /locus_tag="NBH55_002166"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCD1510141.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="NBH55_002167"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="NBH55_002167"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCD1510142.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="NBH55_002168"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="NBH55_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCD1510143.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /gene="era"
                     /locus_tag="NBH55_002169"
     CDS             complement(4412..5311)
                     /gene="era"
                     /locus_tag="NBH55_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCD1510144.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5721)
                     /locus_tag="NBH55_002170"
     CDS             complement(5326..5721)
                     /locus_tag="NBH55_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCD1510145.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="NBH55_002171"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="NBH55_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCD1510146.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="NBH55_002172"
     CDS             complement(6198..8390)
                     /locus_tag="NBH55_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCD1510147.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="NBH55_002173"
     CDS             complement(8414..9385)
                     /locus_tag="NBH55_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCD1510148.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="NBH55_002174"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="NBH55_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD1510149.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3111 a         2419 c         2104 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga aaaa
//
LOCUS       DAHHPA010000084        11251 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.085, whole genome shotgun sequence.
ACCESSION   DAHHPA010000084 DAHHPA010000000
VERSION     DAHHPA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.085"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            158..1609
                     /locus_tag="NBH55_002175"
     CDS             158..1609
                     /locus_tag="NBH55_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCD1510150.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1958..2590
                     /locus_tag="NBH55_002176"
     CDS             1958..2590
                     /locus_tag="NBH55_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCD1510151.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3178..4617
                     /locus_tag="NBH55_002177"
     CDS             3178..4617
                     /locus_tag="NBH55_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1510152.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4912..5130
                     /locus_tag="NBH55_002178"
                     /pseudo
     CDS             4912..5130
                     /locus_tag="NBH55_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5379..5594
                     /locus_tag="NBH55_002179"
     CDS             5379..5594
                     /locus_tag="NBH55_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510153.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5811..6067)
                     /locus_tag="NBH55_002180"
                     /pseudo
     CDS             complement(5811..6067)
                     /locus_tag="NBH55_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6254..7165)
                     /locus_tag="NBH55_002181"
     CDS             complement(6254..7165)
                     /locus_tag="NBH55_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1510154.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7184..8188)
                     /locus_tag="NBH55_002182"
     CDS             complement(7184..8188)
                     /locus_tag="NBH55_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCD1510155.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8185..10308)
                     /locus_tag="NBH55_002183"
     CDS             complement(8185..10308)
                     /locus_tag="NBH55_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD1510156.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10313..11233)
                     /locus_tag="NBH55_002184"
     CDS             complement(10313..11233)
                     /locus_tag="NBH55_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1510157.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3490 a         2115 c         2091 g         3555 t
ORIGIN      
        1 taattttatt ttaaaggcag taacaggatt aaacattgaa acataatcgt tactaaatga
       61 aatcaagaaa tatactgtat tactcaattc attgaaaata tttatcataa tttttaatag
      121 aaaacactat taaagagaaa aaagagaggt aaatcaaatg aaaaaatctt taaaacttgc
      181 atcagcagtt atattaatgt cccaactcct tatagcaaca cctttccatg tgttggcaag
      241 cgaaaattca acatacaaaa ttgcaaaaga aagaaccgag acgactgaaa tgagtaattc
      301 cgatgtaaca gcagagagta gtgaactttt gacaacttca tcaagtgaaa gtagtacgac
      361 ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt caaatgccga
      421 gtcttcaaca acttcatcaa gtgaaagtag tacgacttca aatgccgagt cttcaacaac
      481 ttcgtcaagt gaaagtagta cgatttcaag taccgaatct tcaacaactt caagtagtac
      541 aagtgaaata acttcttcga aagtgacaaa accaaaccat atagaatctt caaaaaagga
      601 agcgccaaaa acggaaggac aaacaaaaac tttttcgaca ccacagcaaa tagaatcaac
      661 agaatctttc caatcacaac caatactatc tggtaatgaa gaaattcatt ttgacaaaaa
      721 tgaatctgta gaaaatttta ttatgaaaat tggtgagtct gcgagaaaaa ttggtcagaa
      781 atatgattta tatgcttctg ttatgattgc acaagcgatc ttagaatctg ccagtggtca
      841 aagccagtta gctcaggcac cgaattacaa tttatttggg ataaaaggaa cttataatgg
      901 aaactttgtc attatggtaa caaacgaaga tttgggtaat ggaacgcttt atacgaccca
      961 atcaaaattt agagtgtatg aaaactatga ggaaagcttt gaagattatg ccaaattatt
     1021 aactaaggga attagtggaa ataaagattt ttatgcagga gcattaaaag ctaatagtaa
     1081 aacatatcga gaagcaacta aattccttac aggacggtat gcaacagata cacaatacta
     1141 tttgaagcta aatgaattaa ttaaaaccta cgatttaacc aactatgata aagaagttaa
     1201 aggtccacaa atgagtaaga ctggatacca agtgcctatt cataattatt caataaccag
     1261 tttgtttgga aatcgtggaa atgaatttca ccgaggactt gatatggcag ctacacaggg
     1321 tgagccgatt catgcagtta aagctggtaa ggttgttaaa gcagagtttc actactcttg
     1381 gggaaattac gtggtgatac aacacgaaga tggatcaacc gcgttatatg ctcaccagca
     1441 acaataccta gtaaaaactg gagataatgt tttgcaagga caaataattg gctatgtagg
     1501 aagtactggg aatagtactg gcccacacct tcatttggaa atttgcaaag atagtagtct
     1561 ttctcagagc atgttagttg atccgaaaac cttattattc ggagactaaa aaaggtaatc
     1621 aatctgacaa agtaacaaga aaatggttgg attcttagac ctttgatcag gatattgaga
     1681 ctggaattga tatctcgatt acgatgggtt tgacaaataa aacaagtcca ttatcgaata
     1741 tcgagtgtat tattgccgtc tttatgctca tattttgagc aggtttcact agatgaaaac
     1801 aatttatcta tcttgaggat ttcgtgtacg tatcgttcag tcttgtattg taaattagtc
     1861 ataaaatcag actaagaaaa attggaaata tttttttctg atttattaaa agttaattga
     1921 aattatatgg tacttctttc ttgggagggt aggtgcagtg aaggaaagat atggtcacct
     1981 tactgttgtt gaggagatta ctcataatgg atataaaaaa tatcgttgcc tatgtgactg
     2041 tgggaatgaa acgattgtat atcgatcaaa cttactatca ggtagaacca aaagttgtgg
     2101 atgtggaaat tataaaaata ggcataaata taaaaatttg acaaataaaa cttttggtaa
     2161 gttagttgca atcaagccaa ctaaactgcg aagtgaagga actgttgttt gggaatgtca
     2221 atgtctgtgt gggagaaaaa agttagtatc ggcaaagcga cttctaaaag gtgatgtaaa
     2281 aagctgtgga tgtttgaaat caaaaaagca tcacattgaa aataaaaaat tcggattact
     2341 tactgcaaag tatcctttgg acaataactg ttcatggaaa acaaaatggg tttgtcattg
     2401 tgagtgtggc agtatgtgtg agataagtta tagtaactta ttttttggac atactcaaag
     2461 ttgtggatgt ttaaaaaggc aagaaacaag gacatgtgtt gaagggacag taatagagtg
     2521 tctaacgtca aaattaccga aaaataatac aagtggtgtt aagggagttt atcgttctaa
     2581 agagaaatag attgcgtata taactttaaa aaaaaagagg tactatttag gatcgtatga
     2641 ttctattaag agtgcagctc aagctagaaa agatgctgaa aataaattgt ttcaaccttt
     2701 gattgacaaa tatcgaggta atcattaatt attgtggata ataattaaca gtgatatata
     2761 aacatctata atatattatt catactattg atatttagta taatcctatg gaattgaata
     2821 tcatagcaat gtatttgaaa agcaaattgg aaaacaaata ggtgcaacac cataaatatg
     2881 aaaaaattgg gaaaaagtag aatggaaatt tgcttacgtc tcacgagata gctcacacta
     2941 ttaatctgaa gaacaatgaa gtcgactttt agaaattgct agaagaaagt aaagcgaatt
     3001 atcactagtt actatagagt aagttcaaat aaagcatctc caacttacat ggttacatga
     3061 ggatatcaaa tcgaaattac tcaagcttcc tatggctaga gtcgcgagaa gttgttgggc
     3121 agtagacata ggttatattg ttcttagcta cacgatcatt agaaaggaaa tcctattatg
     3181 tatagttatc aagaagatgc aaaagtccaa aaaaatcgct ggtggatttt gattgctata
     3241 tctatgttta ccttcatgtc cacattggat ggcagtattg tcaatattgc actacctacc
     3301 atctcagatg atatgggcat accaatgaat caagcggaat gggtagtgtc cgtttatctg
     3361 atgattgtct gttcttgttt gttgctgttt ggtaaaattg gcgatagccg tgggaaaatc
     3421 aaagtatttc ggattggtac gctgatcttt gtcttgggat cgctgttgtg tgggttcaat
     3481 cattcgctag gtattttatt gttcgcacgg gtggtacaag ccattggtgc tagtatgact
     3541 atggctacaa atgccggaat catcacggaa gtctttccca tacatgaacg tggtcgggca
     3601 ttgggagcaa taggtgcgtt tgtttcatta ggctcgattg ctggaccagg tcttggcggc
     3661 ttgattttgt cccaattttc ttgggcgtac attttttgga tcaatgtacc ggtgggaatc
     3721 gtaacgatgt tgattgctga aaaattctta ccaaaagata tcaccatgag cggccataca
     3781 atcgatggag ggggctttgc tgccattgcg gctacgatta tcagcttttt tggagcaatc
     3841 tttttggggc aagaaagtgg ctttttccat ccagtccccc ttgtactgtt cgtaatttca
     3901 gtgattgcct ttgtcagctt tttaaaagtt gaaaagaaag cagcctctcc attgatttct
     3961 ttcggtattt ttaagaacaa gatttttacc atgagcttga ttacagcctt gttgattttt
     4021 tcttccaact tctttgtcaa tgtagtgatt cctttttatc tgcaaaatgc tcgcgggttg
     4081 agtcctagct atgctgggct gttgatgatg gtctttcctt tgatgatggt cgtcggggcg
     4141 cctttaagcg gctacctgac cgaccgggtt ggcccggaga ttttggtatt agtaggcttg
     4201 ggcttattgt cggtaaccca ggtcatgtat atgtttttgt cagtggactc tcctttgtgg
     4261 ctatacattg tcgctactgc catcatgggt gccggcaatg ccttgttcca gtcccccaat
     4321 aatacgatgg ttatgagctc ggtcactatc gaaaacttgg gactggcagg ctcgatgaat
     4381 tcctttgccc gaaatttggg gatggtcagc gggatttcgc tttctacgac ggtcttatat
     4441 caagcgatgt ctgctaagat gggccagcaa gtaactaact atatcgcaaa tcggccggat
     4501 gtctttttat atggaatgaa gattaccttt ttaggcagct ttgttttgtg ttttgttgcc
     4561 tttttcctga ctatctggcg tatcaaaaaa caaggaggta ttcagaaaaa tgtctgaaaa
     4621 aaccactgtt gaagaatttt tggaggcctg cgtatgggtg agtgggattt ccatggatga
     4681 tggaagatga agtgaacttc taaaagccga aaaactcaag tattatcttc aaaatactag
     4741 tgctcaatga gatgcttgat ttgatcacaa gaagactgcg aaagccgctg gcaaccgcaa
     4801 gctaggtatc ccaccgatgg actacgtttt agaggctact ggttatgaat acggagccaa
     4861 cgctctcatc tgcataagaa tacatcaccc agattatgta aagattttcg atacttctgt
     4921 caaaaaatca ttaagataga taaatgattt ccgtctagtc aaattggttc agaatgtaga
     4981 cacacagatg gtaagaagtc actcacaatt cgagaatgga cttgtcctat ttgccgtata
     5041 catcataatc gaaacgtcaa cgatagtatc aatattttga ccaagggcta tgaatacaag
     5101 cattgcctta gaaaacatca agaaccttag gaacaacggg gatagcttgg ttaataagag
     5161 ataccgctgt ttaggcttgc aagtatactc ttttatcaag aattccgtgg ctttagtcat
     5221 gagaggctta atactatgaa aattttcttt agcatttttg atttaggttc cttttctaat
     5281 tataataaga tgaccactac agtaacctac tgaaatatat aaacagccac tgtagtacca
     5341 ttttcaaagt agtctatgag aggagaatct tcattcttat gaacgctgga attgcggata
     5401 tgaatttaat aaaaaaaacc cttaatgatt ttacttccaa ttcaatatca aaaggtactg
     5461 gtatcaattt aagcacaata aaaaagctga aaagtggtga aagatcagtg gaaaagctga
     5521 atcttttaga tgctataaaa ataacggaat ttgctatgaa aaatggaaaa gctgagattg
     5581 aaatatggag ataattatcg tctagtgaat tacttgccac ttagctgaat ttaacgatcc
     5641 ttggtgcttg aagggggaga ttcttgggaa tagagcatac ttgcaagcct atgtctttac
     5701 taacaacgat atctcttatt aaccaagcta tccccgtagt tctaaggctt ttacttttat
     5761 ctaagcaata atcgtattca aaggaaattt ttgaccctta gaaagtctca ttttatcaga
     5821 cgtgagctta ggcattgctg aattttgtta attcggtttt aggttgtgct ttgagcataa
     5881 tatgaaagta ttctttatca cgatttcatt catctaaggt aatgtgatgt gattctgaaa
     5941 ttctttcaaa ggcagttttt gcataagctg acattacatc atcaatgaca tgtttacaat
     6001 gtttcactac taaaataagg gggtaaccaa gaagaaaaac tgaatgatta tttgtatcta
     6061 attccatggt atatcaacca tttcctatat agactgatta tgacatggat taaaataaaa
     6121 aggcactatg tcttaattgt cggttttcga gttaccgaat ggcattcatc acccacctat
     6181 agaggatggg cgacttctgc ctgaaataag ttacactact tataacttct aataaaatta
     6241 gattgagtca ttattattta acatcaacga ttttccagtt tccatttttt tgtaatacta
     6301 atttgtactg aaagtagcta gtggtttttg atacattatc aaggtatttt atctttgtac
     6361 tcactagaaa attttttccg ttttgtagat aaactggatt ctctaactcc gaaaacttta
     6421 agtcaatatt gattggtcgc atcgcaccat tttcaacata atactccaat tctttttcag
     6481 aagctgatgg ataaagttta aaaaatgttt ctaaaaaatc ggtgatttct tccgttgttt
     6541 tggcatcaat ttctgaatcg ttttctactt gttttgcttc atattttgcc ttgtctggct
     6601 ttccaatcat tgttggcaaa ctagttacta aatgattcct gttttcatct tcaaagattg
     6661 tcactcgata tgcagaagaa attgattgcg tcttttttcc ctctgtaata tgttgctcca
     6721 ctaaaaatga aacgacaaat tcttccgagt gtttttcaac atctagaatt tttacagatt
     6781 gaacttcgga actcgtggga atatctgctc gaaccatatc ttggattaaa gcgagaccgt
     6841 tttctgtaag gtactgttcg agattactca ttctcttttc aattgcttct ttgttatttt
     6901 tccatgaaaa atattctttc gcaaaatcct ctgtaaaact ttcaacgcca gaagtattca
     6961 ctaccttctc ttttataacc actttttcat gagttgtatg ttgatcaatg gcggtgaaat
     7021 ttttataaac gccaaaagca agactacaag acaataaaag ccaaagggca agcaccattt
     7081 ttcgatgctt acccacggat acaactcgtt cttttttctt ttttgggctc ttttcctttt
     7141 gattccgttc aatttttatc ttcattcttt ttcctctttt ctcttatttg tatcggccag
     7201 ctccgataag atgctgccgc caatagtttt ccgttagatt cgtccacccc actggatctc
     7261 ctgcatgaaa catgcgattc tcccctgcgt aaatgcccac atgggtcaca taagtaccgg
     7321 catcataggt tgaatgaaag aaaactaaat cacctggttt ggcatctttt agatcaatat
     7381 gagtcatggc atcatattga tcctgagcca cccgaggaag tttcaggtca actttggcat
     7441 aacaccattg ggtcaagcca ctacaatcaa accccgtttc tggacttgag ccaccaaaca
     7501 cataggccgt tccttcatac ttaaatgcct catcaaaaat ttcttggact attttgtcat
     7561 caaactgagt tgtagttagg tattgcttca ctaataaaca gtaaaacata ttgccataat
     7621 tataccgcca cccaccattt tccttaatcg cgattgggtt ggggtaatcg actttttgac
     7681 cacctgattt ttcctttgaa aaagattcag ctaaagcaaa actatgcttc ttcccgtttt
     7741 tcccgacatg atctagatac ccgccaccat agttataggc ctgaataata ctatcttgat
     7801 cacaccctag ttctttggaa cgcttaacca aagacgcaaa atagagacag ccttgtttga
     7861 tggatgcctc ggtacttaat gaattaggtg gtaatccggc tgactcagaa ctttgcataa
     7921 cgtcttccat tgtgccacca gattccactt gaataatcgc caaaagaatt ggaatatact
     7981 ctgttatatc cgattctttg gcatactttt ccaccattgc acggtgtttc aatacttctt
     8041 ctgagacact agttccgcca ggagaagtgc cagaactacc atttccctca ctgtcttcac
     8101 caaacaacaa acccacacac atcaaaatac ccataaaaga gatggtcaga aaagttaatg
     8161 aaataacacc cactttcttc aagttcattt tttctcacct gaccttgatt ttggacgatg
     8221 gttcattctt ttttgtactt tttgattggt ctgctttggc agagtggacc tctgtttcac
     8281 ttgttggaaa gaatttttct tacttgctga ttggcttttc ccttttgaaa tgatttcttg
     8341 actagttagt tttcgtttga tttcgggatt cagatttgct ctcacaatcc gctttggtgc
     8401 ttcttttttc agcatcatat ctttctgagg ttgagctgct ttatctattg gaatattcgc
     8461 ccttcttttt ccctgacgat ttgttgcttc actcagtatc ttgcgttttt cgtccatttt
     8521 cgcatttctt cgttgtcgtt gtttttctcg caaatccact cgatccgctt tttcttggaa
     8581 cagtccgcgc ttgaattctt ctggtgcttt tttcgacttt tcgattcctt tatggagatt
     8641 gtatttcaaa ttcgttggcg tattccttac ctgatccttt acttgtttag tcttatcttt
     8701 caccaaagct tgggtatcca aaactttgcc gatttttctc ccagccaaat tcattcggga
     8761 ttgtttctta ttatttgaaa ctggattctc cttagttaag tcagatagta tttcgtgatc
     8821 gttcggtaat cgtgaattct ttcgtagaga agaaccggat ggcttaaatt tggattttga
     8881 ttgttccttt gccaacttat ttccgactgc tgcaccacca ccaaaagcca aagctcgtcc
     8941 cacatttcga ttgagttgcc gcaattttcg attcattaac atttgcggtt ttcgcatgac
     9001 gcgtcttccc attgactggc tatcattact ctggaggttg aacatactca taatgtcgcc
     9061 caatttgaaa tagataccgg caaaggtcac aatttgcaga aaagcaacca tgaaaaatgg
     9121 ataggttgcc gaaatattga aaaacatcgt ggagatgcta aatgcagtcg taatcaccaa
     9181 agtaacaccc gctcgcatca taatcgtatt gaataaacga ataatcgcct tctttcctaa
     9241 actttcataa gtcggcaaca tggacaataa gaaactaatt ggcagaaaca tcgcaaagat
     9301 aatgaagaga atttgcgaga acaacataat ccctgttaga agaaaaacaa agaaggaaat
     9361 gccaatattg aacaatacca aaaagaccac ggtccctaaa cggttcatgg tcttggtaat
     9421 actgagattc gcattgtcat tgtcttcaat ctctgccttg accgcttctt cccgatcttt
     9481 gcctttgttt tcatctggac tcacagatag aatcttgttg acccggtctt caccaatttc
     9541 ttctatgttg gaatcatcaa attgcagtag taaccaaggt tgttggactt gaatcgaaaa
     9601 caaactatcc cgaatcaaat ccacactatc ttttccttgg ctttctgaat tgggtacaac
     9661 gatctcggtt ccaagggtaa gggccgcttc actaacatct gagctaaagt cattgatttt
     9721 tgtgatatag gtaggcgcat aagcaatgaa cgaacccgat agtagaaaaa tcatcacaaa
     9781 attaatcacg gctcgaactg ctttcgtggt ttctcgtttt aataatccgg tatacgccac
     9841 atacaccccc agagctaaaa tcatcaataa tagaaaccca aaataaaatc cggaggtctg
     9901 gagtccattt tcagtaatac cagccaatgt ttggatattc tttccaatac tttcagccgt
     9961 atccgaaatg aaatctaatt tgtaagcctc ttgaaccaca taacccgtgg cgttcgacaa
    10021 atacaaactc accgtccaaa tgaaattagt aatggcataa agaccataca tcacgctttt
    10081 ccctaagcca tcaccccaat tccaaggcaa ccaatcccaa gagctatcca caaagaaatc
    10141 tagttgatag ttgttcagtg aatatttgga atatagattt cctgcttcaa ctgtatcatc
    10201 aactagccct gtcgcctcgg ctaccgtacc aactaaactc aatagaacta tcagagtgag
    10261 tatgctaata aaaacgattc ccacaatccg aaaaagtttc tttttcatcc cctcactcca
    10321 ttctctcttg aacaggtgga cgggtatcaa aggcatggaa gagttcttca aagactggat
    10381 gtacctgaac cacgcctacc cgaccataca aatcctgcat caagcattgg ccattttcca
    10441 aatctcgtag gcgtttttga ttgctttcat cttctcgatc caagccaaag aattccaagg
    10501 ttttcttgat ttcatgaata tcggtactgc gaaacgcaaa cttcaacccg atattattct
    10561 tcaagcgttc atccagtaaa tcatcactat tttgcgtgac aaagtaaacc ccggcattca
    10621 tagcccgccc agcacgaacg agcttattcg ataatgtttt cccttgcgct acttgaagaa
    10681 aactccacgc ctcatctaag tctacaattt tgaagatttc tcgattacta tggataaagt
    10741 ccaaggcaaa agtggaaatg acgattagca tggaaacact aagcaattcc atggtggtat
    10801 attcttcaaa agtggtttct gcatctggta aaaccaaatc cgctacttga atgatattta
    10861 actgcctatc taaactaatc gactgggtaa tcgaaccatc tgagaaaagc aaatgggcaa
    10921 aatcataatc ggtaaaggac tcgatgtgat ccgcgatatt ttccgcaact ggtgagcctt
    10981 cttttcgaag ctcattaatt acttgtaata atccccgttg attgctttgg gtaaccgtcc
    11041 gaatggcctt acgcaacact ggaaacttct ccccatctcg actagaaatt cctgttaaaa
    11101 aggtcagaat atcaattgct agactttcag aatccttctt tttctccata atcacataag
    11161 gatcaagtaa tccttgattt tttggcttac tagttaagtt gagaatattg atttcatccg
    11221 caatttctgg caaggtttct ttccagccac c
//
LOCUS       DAHHPA010000085        11239 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.086, whole genome shotgun sequence.
ACCESSION   DAHHPA010000085 DAHHPA010000000
VERSION     DAHHPA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.086"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            460..729
                     /gene="rpsN"
                     /locus_tag="NBH55_002185"
     CDS             460..729
                     /gene="rpsN"
                     /locus_tag="NBH55_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCD1510158.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            744..923
                     /gene="rpmF"
                     /locus_tag="NBH55_002186"
     CDS             744..923
                     /gene="rpmF"
                     /locus_tag="NBH55_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCD1510159.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            955..1332
                     /locus_tag="NBH55_002187"
     CDS             955..1332
                     /locus_tag="NBH55_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510160.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1353..1502
                     /gene="rpmG"
                     /locus_tag="NBH55_002188"
     CDS             1353..1502
                     /gene="rpmG"
                     /locus_tag="NBH55_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1510161.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1526..2509
                     /locus_tag="NBH55_002189"
     CDS             1526..2509
                     /locus_tag="NBH55_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD1510162.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2539..2661
                     /locus_tag="NBH55_002190"
     CDS             2539..2661
                     /locus_tag="NBH55_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCD1510163.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2679..4202
                     /locus_tag="NBH55_002191"
     CDS             2679..4202
                     /locus_tag="NBH55_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCD1510164.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4226..4465
                     /locus_tag="NBH55_002192"
     CDS             4226..4465
                     /locus_tag="NBH55_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510165.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4658..5119)
                     /locus_tag="NBH55_002193"
     CDS             complement(4658..5119)
                     /locus_tag="NBH55_002193"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD1510166.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5314..5532)
                     /locus_tag="NBH55_002194"
     CDS             complement(5314..5532)
                     /locus_tag="NBH55_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510167.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5650..7266
                     /locus_tag="NBH55_002195"
     CDS             5650..7266
                     /locus_tag="NBH55_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCD1510168.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7507..8847)
                     /locus_tag="NBH55_002196"
                     /pseudo
     CDS             complement(7507..8847)
                     /locus_tag="NBH55_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9122..9277)
                     /locus_tag="NBH55_002197"
     CDS             complement(9122..9277)
                     /locus_tag="NBH55_002197"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCD1510169.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9573..9905)
                     /locus_tag="NBH55_002198"
     CDS             complement(9573..9905)
                     /locus_tag="NBH55_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1510170.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10419..10610)
                     /locus_tag="NBH55_002199"
     CDS             complement(10419..10610)
                     /locus_tag="NBH55_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510171.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10704..11090)
                     /locus_tag="NBH55_002200"
     CDS             complement(10704..11090)
                     /locus_tag="NBH55_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD1510172.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3741 a         1771 c         2167 g         3560 t
ORIGIN      
        1 ataattttat taaacagcat ctaattttga atacacgaaa gaatcagtat gattgaatta
       61 gttcacttaa tgtctattct tataatataa ggaataatca aagttttaaa aaattggcga
      121 taaaccagtt aaacataaaa gattgaataa aaagttatag tttctttcat attgccgttt
      181 taagtagagg aaaagctact taaaacggta tttgtgtttg attggactaa taaaagtgaa
      241 ttcttatttt tcagagccta aactgcatat tcttttaaat gatttgatta tctctatgta
      301 ccgataaatt cgttttcaaa tagttatcta ttcatatgag ttttatttca tgttatttat
      361 tgttgacaag agcaactctt gttggtaaag tatcagtgtc aaaacgtaac gattacgatt
      421 tgaggtgtct aaatcaaaat aaaaggagta taaaggtata tggcaaaaaa gtcgaaaatt
      481 gctaaagcaa agaaacaaat ggcgatgatt gaaaagtatg cagataaacg tcaggaatta
      541 aaagccgcag gagatcgcac cgctctcgct aaattgccaa gagattcaaa tccgaatcgt
      601 ctgaggcttc gtgatcagac ggatggtcgt ccaagagggt atatgcgaaa gttcggtatg
      661 tcccgtatta agtttagaga acttgcccat caagggttga ttcctggagt aaaaaaagca
      721 agttggtaaa aggaggaaat atcatggcag taccagcaag aaaaacatct aaggcgaaga
      781 aacggttaag aagaactcat caaactgtag taaaaccaga aatttcattt gacgaagcga
      841 atggtgatta tagacgtagc catcatgtat ctttaaaagg gtactataat ggaaagaaag
      901 taattaaagg agcttcaaaa tagtttaata tataactttt aggagggaat caaaatggaa
      961 attatttacc cacctttagt tgaagaagga ttaaagtatt atcttgaaac gacccagcaa
     1021 tcgctagata aatgcacttt ttatcgttcg atggtagaga gaggaattat tactgaaacg
     1081 ggtttaccga cccaacaggc aattgaaaat ggattagtca aagattacta tgaggaccaa
     1141 ggcttatcat ttgatgattt tttaagaatc tatccaattt ttgaagagta tgacgaggag
     1201 ttatttcaat gtatcgatgg ttattgggag atacctgttg atatgaaaga aaatttagtt
     1261 tcacaattgg aatctggcga gttaactttt gaagatgcac aacaaattca agcatatctt
     1321 gaagatcgat aagtattgaa ggaggaagat ttatgcgtca aaatattatt ttggaatgtg
     1381 tcgaaactgg agaacgtctg tatctcacca gcaaaaataa acggaataac cctgaacggt
     1441 tagaattaaa aaagtattcg ccaaaattgc gcagacgggc aatttttaaa gaagtgaaat
     1501 agctaagtgg taaaaaggtg acaaaatggg cataccagtt acaatcgttt ctgggttttt
     1561 aggggctgga aaaacaacgc taatcaatca agccttgcaa gtctcaccct accccaggga
     1621 agaaattatt ataattgaaa atgagtttgg tgaagttggg attgatcaca aattattagt
     1681 tcatagcgag gaacgggtgt tacaactaaa taatggatgt atgtgttgca gtcttcgcag
     1741 tgatttatta gcaatattga tttctttact ggaaacaata gaagaacatc aacaaccaat
     1801 ttcacaaatc atcattgaaa ctacaggtat agcagatccc caaccaatca ttcaaacact
     1861 tttaacagcg cctcaactaa gaggaagagt atatatcgat agtttattga ctgttattga
     1921 tagtgacagt tttgaactta ttgaaagaga accacaagcc cttaagcaac ttgtcatggc
     1981 agatcgcatt tttgtgtcta gaaaacaaaa cttgaattta tctgcactaa aaaaattaca
     2041 gagagaaatc aaaagtatta accctttatc cgatattcga atgttcacac atagtgaaac
     2101 gattcaaaaa aaggattttt ataatttgaa aaaatttaat catcccttag tttttgaaag
     2161 tgattcatca gaaaatcact ataatggcaa atactatcat caacaccatc aagtacatga
     2221 gtttaaatca attctcttaa cttcagatca agacataaaa aaagattttt tactccaatg
     2281 gattgactgg ctgattttaa ccaaccaagg gagcctgtat cgttttaaag gattgattgc
     2341 tttacaagag caggatttta tagttgccat gcaagaggta aacgaacagg ttaattttca
     2401 atacaccact cgacaaaaag accaaaccac tattattcta atcggaaaaa tgttggatga
     2461 agaaaggatc aaacagagtt ttgataaatt actggcggaa agcaaataat gataagtagt
     2521 gattgggggt aatttctaat ggagaaaacg gatatatcta gtgcatatcg acgacttaaa
     2581 agcccgaata ttaaaactag aaaaagggca ttaaaaatta tcaaagaaca taaacgaaat
     2641 aaaatgaaga aattagcata attaaggaag gaaaaagaat gaagaaaatc agtgtaggac
     2701 ttattgggat agcagctcta ggattattgg gagcttgctc cagcactaat gatgcaaaag
     2761 tatcgaatga taaagatgga aaattagaaa ttgtaacaac attctatcca atgtatgatt
     2821 tcaccaaaaa catcgtcggc gatgaagcga acgtggattt aatggttcca gcgggatcag
     2881 aacctcatga ctatgagccc tcagctaagg atatggcaaa agctcatgat gctgatgttt
     2941 ttgtatatca taacgaaaac atggaatcat gggtaccaaa agctaaagaa agttggaaga
     3001 aagctgggcc taatgtagta gaaggaacaa aagacatgat tttattgcca ggtagtgaag
     3061 aagaagatca tgatcacgga gaagaagatc accatcatga actagatcct catacgtggg
     3121 tttcacccaa aatggcaatt aaggaagttt caaatatcaa ggatcagtta gttaaattgt
     3181 atccgaaaaa agcaaaagta tttgagacaa atgctgaaaa gtatttgact aaactgaaac
     3241 ggttggatgc tgactatacc actagtctta aagaagcgaa acaaaaaagt tttgtgacgc
     3301 aacacgcagc ctttggatat ttagctttgg attatggatt gaaacaagtt cctattgctg
     3361 gactaagtcc tgaagaagag ccatcttctg gtcgattagc tgaattaaaa gagtatgtta
     3421 aaaaaaataa gatcaattat atttactttg aaaagaatgc taatgataaa attgcaaaaa
     3481 ctttggccaa cgaagctggg ataaaattgg aagtactgaa tcctcttgaa agtcttacaa
     3541 aagaacaaat ggataatggt gaagattatg tttcagtaat ggaagacaat ctaaaagcgt
     3601 tagaaaaaac aactatggtt gctggtgaag aggtagttcc agaaaaagaa gcgaaggatg
     3661 aaaagacagt tgctaatggg tactttaaag atgccgatgt gaaggatcgc gaattgagtg
     3721 attatacagg cgaatggcaa tctgtttatc cgttgttaaa agatggaatt ttggatgaag
     3781 ttttcgatta caaagcaaaa atcaataaag acatgacagc tgccgagtac aaagattatt
     3841 atactactgg ctataagaca gatattgata ctatcaacat taaagacaac accattgatt
     3901 ttgtagtcaa cggtgaacat catcaataca catacaaata tgttggatac aaaattttga
     3961 attatgaaaa aggaaaccgt ggtgttcgtt ttaattttga aactgatgat gcaggtgctg
     4021 gtcgttttaa atatattcaa tttagtgatc atggaattgc accaagtaag gcagagcatt
     4081 tccatatttt ctttggtgga gaaagtcaag aaaaactcta taatgaattg cataattggc
     4141 ctactttcta tccagcttca ttgtctaaac atgaaattgc tcaagaaatg atggctcatt
     4201 aaaattcaat ttttggagga aatacatggt aagaaaagta ttattgtcta tggattccaa
     4261 gaatattgat gcttatcggc gttatcgatt tgcaaagcaa atgagattaa atgggagtac
     4321 attagctgat attgctgcaa ctttacatgt tagtattgac acagttcata gagtattaaa
     4381 acgggatcct gattgttgtt ttatcaaggt caatactgga agaagtagac gtttagcaaa
     4441 acggcataat agcttggcca attaattgat aataccagag tagatcattt ttcccttatt
     4501 tggatttgat ctgcttttta ttatgtcttg aattgtcaaa tcaagcgcaa cactttatca
     4561 aagaagactt cataaggtgt tctccaatct aaacactttc taggtctgtt attcagtttt
     4621 tcaatcatct gatttatcgt tgagttatct accgtagcta tctctttcga ttttggcaag
     4681 tattctcgaa tgagtctatt cgtattttaa tttgttcatt gttgccaagg gcatgaggat
     4741 ctggaaaata aaagggcata ttattcaatg cttctgtaac tgcgaaatga ttggagaatt
     4801 cttttcctct atctggagtg atcgttttga ccgcctcatt tggaatactg cttaaaagtt
     4861 caatcatttt ctcgctcaca taagcggctt tctttctggg gaccttcttt cctgctaaaa
     4921 ggaagcatga ttttctgtct gtcaaagtca ctaaacaagt accaccagtt ttgccagcta
     4981 caatatcggc tttccaatga ccaaaatcac ttctatcatc tgcggatttt ggacggccat
     5041 ggatcgaatg agtaaggcaa atctttccac gagactcgac atgatttttt gtatgtcttg
     5101 tttttcctcg atggtgcaat gaacggatca ggcctttatt gccgtgtgag aggggttctg
     5161 tatcgaaaag atcattgcaa attgcacgtt agatcgtgat aaaactaatt gaaaagtcca
     5221 tatgttcatg cttcaaacga ttcgagattt cctctggcga ccactgaaaa tcgacaaata
     5281 aatgacgaac aattgaccaa actcacggat catttagaat gtgataacgt ccgcattttt
     5341 ttcttcgata agcataattt ttttccgctt tggaaggtga atagtttttt tcttgtttct
     5401 tctcagttca tgtaaaatag taaacgggct atgctgaatg tcgttgccaa ttatactaat
     5461 agaaaatcct tcttcgtgaa gcagaaagat tctttctcgt tcgagtatag taagatgacg
     5521 atactggctc ataattattt tccttctagg agtgttctcg acaaacttat actagttgga
     5581 tatcgattat gactcatttt tatgtgttgc acttaaagtg taaattcaag gtatagaaag
     5641 aggttcccta tgaaaaaaat acctattaca atcttaactg gctatcttgg ttcaggaaaa
     5701 acgatacttc taaatcatat tctgtacggt gatcacggaa aggaaattgc tgtcattgta
     5761 aatgacatag gggaagtaaa tgttgatggg gtattagtcg acaagaaagg ttttaaaaga
     5821 actggagaaa aacttgtttc gatgccgaat ggatgcattt gttgtacatt acgcgaagat
     5881 ttgattgttg aactagatca attagtgcaa ttttctgact tgaatcaaat tgttattgaa
     5941 gcttcaggaa tcagcaaacc aattccaatt attcaagcaa ttatattagc taaaaaccct
     6001 ttagacattg atttgaccca acgattagag attggtgcca ttattatagt agtggatgta
     6061 aaacgttttt gggataattt cgaaacgggt gaaaagattc aggaaagaaa agctgaaggt
     6121 aatgtagttg aagaagaaca ggatattcgt ggcttgttaa ttgatcaatt cgagtgttgt
     6181 aacatattac ttttaaataa tagtgactta gtgtcaacca agacgattta caaaattaac
     6241 ggtctaattg ctaagttaca gtccaatgcc aaaataatta ggacaattaa aggtgaagta
     6301 gacttatcga aaattctaaa tacaaatctt tttaatatag atgagacttc ctattcagtg
     6361 ggctggttca aagagttaga gcttggctat caaaatcata ttcctgaaac agaagaatat
     6421 ggtatctcaa gttttatcta tcgaaatcgg ttactccttg acgcacaaaa atttgcccat
     6481 cttacggata aaaattttcc tggcaatatt acaagagcaa aagggtttct ttggtttgcc
     6541 caacatagtg atttttcgac gatctttgaa acatcgggta gctataaaag tattgaatca
     6601 ttaaattatt gggtagctag tttacctaaa attgagtgtc aaatgattat acaaaaaaat
     6661 cccgaattga aggaagcctg gcatccgttg tttggtgaac gcattaataa actagttttt
     6721 attggtatga agatggatca acaatgtatt aaggaacaac ttgagggttg tttaactgat
     6781 tctcttaatg cagataattt ccgaaaaatt tcaccatttc catggaaaaa gtctaatatg
     6841 tttagccaga tatcgttaat gaaattttta agaaaagctg gtgtcaagat aggcgatgtc
     6901 ataattattg gcattttgat tattacctct tttattccct tatttgtatt ctctatagaa
     6961 aaggagaatg aaacaactga agatgctatc aaatatgcca tcgttcgaat tgatggcaag
     7021 gaaatggatc gcttcgattt ggataagatc aatcataaac tcgtgaatta tcatcctgcg
     7081 aagggacaat ataatattgt tgagattaaa gatggaagaa ttcgagttaa agaagataat
     7141 agtcctgatc aaatcgcggt tagaactgga tggatctcaa aacccggtca aacgagtatt
     7201 tgtttacccc ataagttagt tattagtatt gagaaaaaag aaagcacaga ttactacatt
     7261 tactaatctg gaaatagatg aggaggtttt taataagaaa aagatattat tattaataat
     7321 agcaggggct ttattaagtg tgttaagcgc ttgtggacaa gaaaaagaag cggtgacaac
     7381 aggcacaagt atctttaacg ggacctgcaa tggtttagtt aacagtcggt tagattactt
     7441 attttggctc ttcgtcaatg agtgttggtg aagaaattca atagaataat cgagatactc
     7501 acacggctag acagctatct tgtctagccg tgttgttgtt ctggctttag tcagttgggt
     7561 agtattctta gttttcttaa aatattttcc tcgatataaa aagattcgtt ctcggtagtt
     7621 ttgaaagttt ctatagccat aggcaattcg ttttagtact ttgatatggt tattcaaaca
     7681 ttctataggg ccgttggaat agggaacgcg caacgctcgt ttgatttctg tttggtattt
     7741 cttgaaggtc gtgaacactg gttggtagtg ctctggcaac tccttgtagt cttcttttag
     7801 gagcgcgacg aattcaggaa catcttgtct tctgataacg gaaagaaaat cctggtagac
     7861 ttcgtagcct cgctttaagg aatcatcata agataatagc cgatcgacta tctctgcttc
     7921 tgtcagatga tctcgaaatg acggacgcca gtagcgatgc tgacctttca attcccacgc
     7981 attcttttga agtagtttcc agtatttctt taagtgcttc gaccgacgat cccctttttt
     8041 aaaggtcttc attacttgaa ttcggtggtt ggtaaaagcc cgaccaatgt gttggctaat
     8101 atgaaagcga tcggcgacga ttcgggcatt cggaaaacac tctttcacca atgtgcgata
     8161 gggtgtatag aaatctgaaa ccactagacg cacttgttca cggactttta tagggtagcg
     8221 caagaaatag tcgcgtaaag gattgagttg ccgattttcc acaatatcaa tcagttgatg
     8281 ggtcttgccg tccatcatga caaagctcat ggctcccgag acatttttca ctgatttgaa
     8341 ttcatcgaaa cacaccactt ctggtaacag ctggctcaaa gaaatggttt taggccgaag
     8401 gctgcgaagt attcgaatga cagtggtcgg agaaatgtgc ttcatacgtg cgatcaaaga
     8461 catagaaaga ggttcgctta atagctcaag aatggcttgt ttgactctgc gagcaatgga
     8521 gcagcgacga tcactaactg aatcttctgc taaaaaagtc tttccacagt gtttacattt
     8581 gaagcgctgt ttgttaatcc gcagaatggt cttgtagtta ctgacatcat tcaataagat
     8641 ggatactttt ttccagccct atttaatgat cgtttgttca ttttgatagt ggcgatgaaa
     8701 gcattcctcc ggttgatagg tcaatgtgcc atcaaaaaca aaacatgttt cgtcattgat
     8761 tcgctctttg cgaaaggcat cttcatgaaa gataagattt aggtctagaa tatctagtaa
     8821 ctctttggta aactctgaat aggacatctt gaaacactcc tttgaatggt tttcgtcgac
     8881 tttattctaa aggattttaa ggtgtctgtt tgcgtttatt agaaaaaaga aaaagtattg
     8941 gcaacagaag gcgtcaccaa cactagatat tatagatccc tattttcttc gcttcaaatt
     9001 gtcaaggagt catcataaat tttgtatgga ttcttttgtt tgggtagtag tcttctaaat
     9061 aatcttggat taacaccttc atctctattt tacttttata tttgttggag tcgattaatt
     9121 ctcatttgat tgatcgatga aacgcttcaa ttactgcatt gtcaacagga gtcccagttt
     9181 ggctcactga atgtgtgatg tggtggtcgt tcaacagctc ttgataaata aaagaccgat
     9241 gctggctacc tctatcggaa tgaattaccg taggcaattc tccttaataa atttgtaatg
     9301 gacggacgac aatctctttt ttcatgtgtg aatctaccat atagtaacgt agaatacgag
     9361 ttgctaagtc aatataggtg gacaaataga ggcgaccgtc attacatgga atgtaagtaa
     9421 tatccatcac ccaaacagca tcaatttggt tctgttcaaa ttatcgattc aacatttttt
     9481 taccatttct ttgattgttt tttctctacc atgagaagat ttgcgtcgat gaaatcctct
     9541 tgtgcataaa ttcatttcac gcataagaac agctacaaca cgtttattga tttcgattcc
     9601 ttcatcgtaa agtttttgag tgatccctgg agatccgtat cgatctttgt gttcgtagaa
     9661 cactcgtctt cctcttttag tcgtttattt tcctgttcaa gaatgtagaa atctgcttta
     9721 tcaggtaatc tttttccttg tccagcaaaa gcatttttcc cgtatcgacg atattctgaa
     9781 acccatcggg aaattgttgg acgagagata ttgtattctt cagcaatttt cttggtagtt
     9841 acttttttct caaggatatc ctgtgaaatc tttagtttaa atttacgatc atatgttttt
     9901 ctcaatagag ttcattcctt ccggtaacgt aactgatagt caagaaaagt gtatcatagc
     9961 ccaacttagt atcagggttt gtcaactaaa ctgtggaagt tgatagataa gagtttttaa
    10021 caaccaaaat tctcttgact atttttgaaa tcagacaaat tttgattgga cacatagttg
    10081 aataaatcta ccgtcgttac ggaaggtaaa tcgcatgctt ttcaattcta gaggagaagt
    10141 cctcgttgaa gtaccatagc ccagttctgg attcgacctc ctgcccactt catacgtaaa
    10201 taaagtaatt ttaggagtga gactgtgaca aaagttattt ttaagctaaa aacgaattaa
    10261 aaaacgttga taattaaaat cattcgtttt agattctatg aatcattata gaacgctgta
    10321 aaatcgatac tttataatta taaagaagat agtttcaaat ttattgttcg aaatttgaaa
    10381 ctatcttttt acttatgtca cagactccct tagttgagtt attctttaat ttcgaatttc
    10441 gagatagcta gatatattgc aagcatacat tcttgataac aatcttgatc atattttcca
    10501 gttctccagg attgcttatg aatcaaagag ctaaatcttt tgagaattgc ctcaacagct
    10561 tcttcatttc cagcctttgc taaaatgaaa tcttctttta aagaattcat aaatctacct
    10621 tcttcccatg gacttataat ctatttcttt gggaaggagg agaacgagca aagaagcata
    10681 caaaattagt tattatgcag atattaaacc agcatttttt gaattttttt gtaaagtcga
    10741 tgttttaaat ttgtcgcacc ctgtcttgta atccccagaa cctttccaat ttctttatca
    10801 gttttatcaa agataaattt ttcgataaga aaaatttgtt caggttcttt caacaaagga
    10861 aggatttttg ctaattttat atcttcaaca aggagagtca catttaaaac attaatcggt
    10921 tgtttaaatt ttatagtatc tttagtaaac aaatatggtg ggagttgatc aagttgttca
    10981 tgtttttgaa tttcttcttg ttttttataa taagtattag cggtgttcaa aatgaccttt
    11041 tcaaagaatc gaatcaactg ggcatttacg cgttcgtcgt tatctgacat attgcacacc
    11101 tcctccatta agatttaaag ggaaaagagg caaaaaacac gaaaagaagc taatgcaatt
    11161 atgctgcgct gcgaataact tctataagct cacatgcatt gataggactg ctgatgttta
    11221 ttttcaaaaa tgttttttt
//
LOCUS       DAHHPA010000086        10769 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.087, whole genome shotgun sequence.
ACCESSION   DAHHPA010000086 DAHHPA010000000
VERSION     DAHHPA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.087"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="NBH55_002201"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="NBH55_002201"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..279
                     /locus_tag="NBH55_002202"
     tRNA            207..279
                     /locus_tag="NBH55_002202"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Val,seq:tac)
     gene            283..355
                     /locus_tag="NBH55_002203"
     tRNA            283..355
                     /locus_tag="NBH55_002203"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Lys,seq:ttt)
     gene            363..444
                     /locus_tag="NBH55_002204"
     tRNA            363..444
                     /locus_tag="NBH55_002204"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:397..399,aa:Leu,seq:tag)
     gene            462..534
                     /locus_tag="NBH55_002205"
     tRNA            462..534
                     /locus_tag="NBH55_002205"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Thr,seq:tgt)
     gene            546..617
                     /locus_tag="NBH55_002206"
     tRNA            546..617
                     /locus_tag="NBH55_002206"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Gly,seq:gcc)
     gene            634..719
                     /locus_tag="NBH55_002207"
     tRNA            634..719
                     /locus_tag="NBH55_002207"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:668..670,aa:Leu,seq:taa)
     gene            730..803
                     /locus_tag="NBH55_002208"
     tRNA            730..803
                     /locus_tag="NBH55_002208"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Arg,seq:acg)
     gene            813..886
                     /locus_tag="NBH55_002209"
     tRNA            813..886
                     /locus_tag="NBH55_002209"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:847..849,aa:Pro,seq:tgg)
     gene            906..978
                     /locus_tag="NBH55_002210"
     tRNA            906..978
                     /locus_tag="NBH55_002210"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:939..941,aa:Ala,seq:tgc)
     gene            1006..1079
                     /locus_tag="NBH55_002211"
     tRNA            1006..1079
                     /locus_tag="NBH55_002211"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1040..1042,aa:Met,seq:cat)
     gene            1093..1166
                     /locus_tag="NBH55_002212"
     tRNA            1093..1166
                     /locus_tag="NBH55_002212"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1127..1129,aa:Ile,seq:cat)
     gene            1190..1279
                     /locus_tag="NBH55_002213"
     tRNA            1190..1279
                     /locus_tag="NBH55_002213"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1226..1228,aa:Ser,seq:tga)
     gene            1292..1365
                     /locus_tag="NBH55_002214"
     tRNA            1292..1365
                     /locus_tag="NBH55_002214"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Met,seq:cat)
     gene            1384..1459
                     /locus_tag="NBH55_002215"
     tRNA            1384..1459
                     /locus_tag="NBH55_002215"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1417..1419,aa:Asp,seq:gtc)
     gene            1461..1531
                     /locus_tag="NBH55_002216"
     tRNA            1461..1531
                     /locus_tag="NBH55_002216"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1493..1495,aa:Gly,seq:tcc)
     gene            1545..1618
                     /locus_tag="NBH55_002217"
     tRNA            1545..1618
                     /locus_tag="NBH55_002217"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1579..1581,aa:Ile,seq:gat)
     gene            1643..1730
                     /locus_tag="NBH55_002218"
     tRNA            1643..1730
                     /locus_tag="NBH55_002218"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1677..1679,aa:Ser,seq:gct)
     gene            1752..1823
                     /locus_tag="NBH55_002219"
     tRNA            1752..1823
                     /locus_tag="NBH55_002219"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1785..1787,aa:Glu,seq:ttc)
     gene            1846..1935
                     /locus_tag="NBH55_002220"
     tRNA            1846..1935
                     /locus_tag="NBH55_002220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1882..1884,aa:Ser,seq:tga)
     gene            1948..2021
                     /locus_tag="NBH55_002221"
     tRNA            1948..2021
                     /locus_tag="NBH55_002221"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1982..1984,aa:Met,seq:cat)
     gene            2040..2112
                     /locus_tag="NBH55_002222"
     tRNA            2040..2112
                     /locus_tag="NBH55_002222"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2073..2075,aa:Asp,seq:gtc)
     gene            2116..2188
                     /locus_tag="NBH55_002223"
     tRNA            2116..2188
                     /locus_tag="NBH55_002223"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2149..2151,aa:Phe,seq:gaa)
     gene            2193..2263
                     /locus_tag="NBH55_002224"
     tRNA            2193..2263
                     /locus_tag="NBH55_002224"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2225..2227,aa:Gly,seq:tcc)
     gene            2276..2349
                     /locus_tag="NBH55_002225"
     tRNA            2276..2349
                     /locus_tag="NBH55_002225"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2310..2312,aa:Ile,seq:gat)
     gene            2361..2432
                     /locus_tag="NBH55_002226"
     tRNA            2361..2432
                     /locus_tag="NBH55_002226"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2394..2396,aa:Glu,seq:ttc)
     regulatory      2621..2700
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2698..3891
                     /locus_tag="NBH55_002227"
     CDS             2698..3891
                     /locus_tag="NBH55_002227"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCD1510173.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            4021..5493
                     /locus_tag="NBH55_002228"
     CDS             4021..5493
                     /locus_tag="NBH55_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1510174.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5576..6364
                     /locus_tag="NBH55_002229"
     CDS             5576..6364
                     /locus_tag="NBH55_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCD1510175.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6431..7099)
                     /locus_tag="NBH55_002230"
     CDS             complement(6431..7099)
                     /locus_tag="NBH55_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCD1510176.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7214..8926
                     /locus_tag="NBH55_002231"
     CDS             7214..8926
                     /locus_tag="NBH55_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1510177.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8929..10701
                     /locus_tag="NBH55_002232"
     CDS             8929..10701
                     /locus_tag="NBH55_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1510178.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3095 a         1966 c         2500 g         3208 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgcttaagtt atccatggag gtttagctca gctgggagag catctgcctt
      241 acaagcagag ggtcagcggt tcgatcccgt taacctccat atgagccgtt agctcagttg
      301 gtagagcatc tgacttttaa tcagagggtc acaggttcga gccctgtacg gctcatattt
      361 ttgcgggtgt ggcggaattg gcagacgcac tagatttagg atctagcgcc ttacggcgtg
      421 ggggttcaag tcccttcacc cgcataattt aagttttaca tgccggctta gctcagttgg
      481 tagagcatct gatttgtaat cagagggtcg agggttcaag tcctttagcc ggcatcttta
      541 aacacgcgga aatagctcag tggtagagca ccaccttgcc aaggtggggg tcgcgggttc
      601 gaaccccgtt ttccgctttg ccaaagcaat cgcgccgggg tggcggaact ggcagacgca
      661 caggacttaa aatcctgcgg tgagtgatca ccgtaccggt tcgattccgg tcctcggcat
      721 ttcaaatatg cgcccatagc tcaactggat agagtgtttg actacggatc aaaaggttag
      781 gggttcgact cctcttgggc gcgttaatta aacgggaagt agctcagctt ggtagagcac
      841 ttggtttggg accaaggggt cgcaggttcg aatcctgtct tcccgataat tataaattta
      901 aacacggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag gtcagcggtt
      961 cgatcccgct aggctccatt tcatttaaat aatgaatatc tttttcgcgg tgtagctcag
     1021 ctggctagag cgtccggttc atacccggga ggtcgggggt tcgatcccct ccgccgcgat
     1081 ttttaagagc ctggaccttt agctcagttg gttagagcag acggctcata accgtccggt
     1141 cgtaggttcg agtcctacaa ggtccattgt ggcgttgtaa gtttcatatg gaggattacc
     1201 caagtccggc tgaagggaac ggtcttgaaa accgtcaggc gggtaaaacc gtgcaagggt
     1261 tcgaatccct tatcctcctt tttcatatta tcgcgggatg gagcagtctg gtagctcgtc
     1321 gggctcataa cccgaaggtc gtaggttcaa atcctgctcc cgcaattaaa aactaaaaac
     1381 actggttccg tggtgtaggg gttaacatgc ctgcctgtca cgcaggagat cgcgggttcg
     1441 attcccgtcg gaaccgccat gcgggtgtag tttagtggta aaaccacagc cttccaagct
     1501 gttgtcgcga gttcgattct cgtcacccgc ttttagtttt ttatgggcct atagctcagc
     1561 tggttagagc gcacgcctga taagcgtgag gtcgatggtt cgagtccatt taggcccatt
     1621 agtacaaaat cttattggtt ttggggaagt actcaagtgg ctgaagaggc gcccctgcta
     1681 agggtgtagg tcgggaaacc ggcgcgaggg ttcaaatccc tccttctccg tttcaatatt
     1741 ttgttttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg gcggtaacac
     1801 gggttcgaat cccgtacggg tcattaattt aatatagttt tacatggagg attacccaag
     1861 tccggctgaa gggaacggtc ttgaaaaccg tcaggcgggt aaaaccgtgc aagggttcga
     1921 atcccttatc ctcctttttc atattatcgc gggatggagc agtctggtag ctcgtcgggc
     1981 tcataacccg aaggtcgtag gttcaaatcc tgctcccgca attaaaaact aaaaacattg
     2041 gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg ggttcgattc
     2101 ccgtcggaac cgtttggctc ggtagctcag ttggtagagc aatggattga agctccatgt
     2161 gtcggcagtt cgattctgtc ccgcgccatt atgcgggtgt agtttagtgg taaaaccaca
     2221 gccttccaag ctgttgtcgc gagttcgatt ctcgtcaccc gcttttagtt tttatgggcc
     2281 tatagctcag ctggttagag cgcacgcctg ataagcgtga ggtcgatggt tcgagtccat
     2341 ttaggcccat ttattttatt ggcccgttgg tcaagcggtt aagacaccgc cctttcacgg
     2401 cggtaacacg ggttcgaatc ccgtacgggt catggaagat actttttgag tatctttttc
     2461 tttttatctg aaagaatgat tttaaaaagc ttagcagagt tatctttatg gatgattgtg
     2521 ctaagctttt ttggacagtt ctaatgtaaa atatttttat attttttaag caaaaaaatg
     2581 aaaaaatacg agcagaaaac tgttttatct gctatacttg gttcgttgca agttcccgaa
     2641 aggatccgca ggacggagat gccttgtaac cgaatttaac tagggggaat cattataatg
     2701 gtagaaagac acttatttac atcagaatct gtttctgaag gacatccaga taaaattgct
     2761 gaccaaatca gtgatgcaat cttggatgca attttaaaac aagatccaac agcacgagta
     2821 gcgtgtgaaa cgtctgtaac aactggtctt gttttagtat ttggagaaat atctacgacg
     2881 gcatatgtag atattcaaaa agtcgtacgg gaaacaataa aagaaattgg ttatacacgt
     2941 gcaaaattcg gatttgatgg agatacagca gctgtattgg ttgcgattga tgaacaatct
     3001 cctgatattg ctcaaggagt tgatgaagca cttgagatcc gggatgaaga taaaaaagac
     3061 gtattagatg agattggtgc tggtgaccaa ggtttaatgt ttgggtttgc tgtagatgaa
     3121 acaccagaat tgatgccttt accaatcgct ttgagtcatc gtctggtacg acgcttggca
     3181 gatttgcgta aatcaaatga attaacgtat ttacgtccag atgcaaaatc tcaagtaacg
     3241 gttgaatatg atgatcaagg acaaccggaa cgcgtagata caatcgttat ttcaacacag
     3301 catgatgatg cagtagataa tgaaacaatt cgtcatgatg tcatcgaaaa agtagtgaag
     3361 gaagttattc cagctgaatt attagatgat caaacgaaat attatatcaa tccgactggc
     3421 cgatttgtca ttggtggtcc tcaaggggat gccggattaa caggaagaaa aatcattgtt
     3481 gatacgtatg gcggttatgc tcgtcatggt ggcggtgctt tttctggtaa agatgcgaca
     3541 aaagttgacc gttctgccag ctatgctgca cgttatattg ctaaaaacat tgttgcagca
     3601 gggcttgctc gcaaagcaga agtacagcta gcttatgcga ttggtgttgc tcaacctgtt
     3661 tcgatctcaa tcaatacttt cggtactgga acggttccgg aagaagaact gattgctgca
     3721 gtgagagaaa acttcgatct tcggccagca ggaatcatcg agatgcttga tttgcgtcgt
     3781 ccgatttaca aacaaacagc tgcttacggt cattttggtc gtacagatgt agatttgcct
     3841 tgggaacata cggacaaagt agatgcgttg aaagcaagtt tagcaaaata agttatattc
     3901 agctggaacg atcgccaagt ggtggttgtt ccagcttttt ttatggcagt taacctcttg
     3961 aaaaagatag taattattta tctattttta gatcctgtca taatgaaagg gaggaataag
     4021 atgaaaaaag aaacaaatgt tactttggtc acagtgagta ttttcattgc aacgttcatg
     4081 acggctattg aaggtacaat cgtaacaacc gctatgccaa cgattgtcgg ttcattacat
     4141 ggaatggaaa tcatgaactg ggtattttcg atttatttgc tgacaaacgc tatgcttact
     4201 ccgatttacg gaaaacttgc ggataaagtg ggacggaaac caatctttat ttttgggaca
     4261 ttattgttta ttatcggttc gttattttgc ggtttatctc aaagcatgat tgggttgatt
     4321 gctgctcgtg cgattcaagg gatgggtgct ggggcaatga tgccagtagc tttgacgatc
     4381 atcggcgatc tttattctat tgaaaagcga gcaaaagttt taggtttgaa tagttcagcc
     4441 tgggggattg ccagtgtttt tggaccgctt gcgggaggat tcatcgttga aacgatcagc
     4501 tggcactgga tcttttttat caatgtgccg atcggaattc tattaattat tttgatttgg
     4561 ctatatttaa atgaaccaaa aatcatacat gagtctaaac caatggatat ttttggtagt
     4621 tttagtttga tggccatgct tgtctcattg ctgttagggt tccaattatt aggagacgaa
     4681 ggaatgagtc cgcttgtttc tgctttgctg ctaggtagta tcgtaatgtt aggtattttt
     4741 gtttttgccg aaaaaagagc taaagatcct gtcatcatga tgcatttatt caaaaatcca
     4801 ctatttgttg tggtcaactt ggctgcagca ttggctagtg gttttttgat gggaatcgat
     4861 gtttatatcc ctatgtggat gcaaggtgta ttagggctga atgcagcaat tggtgggctg
     4921 gtattagcac cattatcact agtctggatg gtcggttctt tccttgcaag caagtggatg
     4981 actcaaatga aaactcattg ggtcttgaga attggactac taatcgcagc tattgggggc
     5041 gcttggttgt atcttatgcc tttcagaagt aactatttat ggttttttgc tatatcaacg
     5101 gtcatcggta taggcttagg tgtgaccatg actacctcga ctgtcagtgc acaaaatagt
     5161 gtatctcgtg accagcttgg tgtagcaact tcttttaata cgttagttcg aacaattggt
     5221 caaacagtca tggtttctat ttttggttta ttgataaatg gggtaactaa acgagaactt
     5281 gcagcagcaa atttaacaaa tgatgcagat atcatgaata aactggtgaa tccacaaacg
     5341 gctaaatata ttgatgcgga tattttgtta ccattaagaa aaattcttta tgatgggtta
     5401 cattccgttt atctagttgg cttgttactc gtgatcattg ctttggtctt aacgcttttc
     5461 gttcatgaaa aaagtggtaa aataaaacag tagtttcggt taagatatgt acgtagccga
     5521 atcattcaaa tttaaagtta ttcaaaaata acttcttact aaggaaggcg aaaatatggc
     5581 atttattcaa gcaaatattt attcgaatgt gttagaaatg gaagtcaatc taaatgtgat
     5641 actaccgcaa acaacaaaaa aggtgattgg caccagttca gagcaagaga caacggatat
     5701 acctgtttta tatttacttc atggaatggg tggcaatcat agtgtatggg aaagacgtac
     5761 gtcgattgaa cgttatgtag caaactacgg attagctgtc atcatgccat ctactgatct
     5821 tggctggtac acagatacag tttatgacat gaagtattgg acattcattg cagaagagct
     5881 acctgagatt tgtcatgaat tattcccgca attaacaaga aaaagagaaa aaacatatgc
     5941 cgctggatta tcgatgggcg gatatggcgc gttaaaacta ggccttgcaa aatcagaaaa
     6001 tttcgcagca gtaatctctc tttcaggcgc tgtttctgta ggaaatcgaa tggatgatct
     6061 tttgatggtt cgtaaagcat ctttttggga agggatcttt ggaccattgg atcaaatcga
     6121 aggttcaaaa aatgatccgc aatacttatt gaaagaacta gtaaaaagcg gaaaaccagt
     6181 tccaagattt tatatggctt gtggggaatc tgattttctt tatcaggcaa atcaggaaat
     6241 ggtatctgct atgcaggaac aaggcttggc ggtcaccttt gaacaaggac ctggcgatca
     6301 taactggatc ttctgggacc aatggattca acgtgcattg gcgtggctaa tggaagaaaa
     6361 ataaaaaaag agtcttaaaa agatagctcc tatagagatc acaaacatag tgatctctat
     6421 aggagctttt ttactgctcg ttttctcttc ttttattttg ttggcataag ccttcaatat
     6481 aggtttgtgc actcgttaga aagagtgctt tttcattcaa ttctgcatgg gactgcatac
     6541 tttctttaaa tccctctaat tgattttcaa ctacataatt acgcataaat agaactgttt
     6601 ctttcaaaaa ttcatttccg tccataattt tttgtcgaag ctgctgttca tgctgctgat
     6661 ccattaactg tccaatcaat aaattgtgga gagaggcaat ggcctgatga ctttgggtga
     6721 ctgaaaatcc ttcacgaaca agtaattgga tcatttgctc caaatgttct aatcgactcg
     6781 gaaaagcggg cacagtattg atttctaatt ctgccccaca agggaaacgt gtataaaggt
     6841 catagtaatt ttccatatac tgcagcaact gttcattcca aggcaaagtt ggatctggaa
     6901 gcgctaattc ttcttccatt gtttcagcca ttttttgcaa gagctgttgt ttgtttttga
     6961 aataccaata taaagcaggt gcttgaacat gaattctgtt tgcgatgttt cgcatggaaa
     7021 gctgcgaaag agcttgtgtt tcagccagga tatcaaaggc ggcctgaatg atgattgctt
     7081 gagaaagctt tatttccatt ttatcaacct cgtgtattgc attatctttt taaacatggt
     7141 aaattgttag tcggtctaat tttacagtgt taaattagtc ctgtcaattt aaacaaaggg
     7201 gtgtaatcaa cgaatgaaat taatcttacg acatgcgaaa aaatataaag tagctgtttt
     7261 tatttccttg ctgtccgtag cagtcatggt tgcagcagca ttatggcaac ctaagttact
     7321 acagcaagta ttggaagcta ttattacaga agataatgat gaaatgaaaa caattggtat
     7381 ttacctgatc agtctggctt tgctaggatt agccgcaggt gtaacgaaca cgattttctc
     7441 tgcaaaagta gctcaaggcg taagtgcaga tatccgagaa gaagcatttc gaaagatcca
     7501 gacattttca tttggaaata tcgaacagtt ctctgctgga aatcttgttg ttcgtctaac
     7561 aaatgatatc acgcaaatcc aaaatttagt gatgatttct ttgcaatcac tttttcgaat
     7621 tccattctta tttattggtg ccttcatttt agctatgatc acgatgccgc aattatggtg
     7681 gatcatcgta gcgcttatcg ttacggtatt tctgattacg gcactttcat ttacaaggat
     7741 gggcaagcac ttcatgatca ttcaaaaact gattgatcgt gtgaatagca ttgcaaaaga
     7801 aaacttacta gggattcgtg tagtgaaatc ttttgtacaa gaagaaaacc aattgaagtc
     7861 attcagcaaa gtaagtgagg acttaacaaa acacaacatc atagtcggta cattattttc
     7921 tgtgatgatc ccgtccttca tgctggctgc caatttggct gtggttgggg cgatcttttt
     7981 tgtgagcgat cttgttaaag atgatcctac gttaattggt ggaattgctt cattcatgaa
     8041 ctacttgatg cagattatga tggcaatcat tattggcgga atgatgatga tgatgacttc
     8101 aagagcagcc gtatcgctga agcggattgc ggaaatttta gatgctgaac cagatcttac
     8161 ttatttagac gttccagaac aagagttgac gggttctgtc acttttgacc atgtttcttt
     8221 tcgttatcct ggtgaggata cagatacgct taaagatatc agcttttcta tcaaacctgg
     8281 agaaatggtt ggtatcgtcg gggcgactgg tgccgggaag tcaacactag cacaattgat
     8341 ccctcgtcta tttgatccga cagaaggcaa agttgaagta ggtggtgtag atcttcgtca
     8401 agtaaatgaa aagagtttaa gacaaacggt ttcctttgtt ttgcaaaaag caattttatt
     8461 ctcaggaacg attgctcaaa acttgcgcca agggaaaaag aatgcaacag aaaaagagat
     8521 ggaacatgct tcttctattg ctcaagcaaa agaattcatc gaaaagttat ctgatcgtta
     8581 cgaagcacca atagaagaac gtagcagtaa cttttctggt ggtcaaaagc aacgtttatc
     8641 cattactcgt ggcgttatcg gtaatccaaa gatcttgatc ctagatgaca gtacgagtgc
     8701 actggatgct cgctctgaaa agctggtccg agaagcactc gatcgagatt tgaagggaac
     8761 aacgacgatt gtcatcgcac aaaaaattgc ttctgttgtc aaagcagacc gcatcttagt
     8821 attggatgaa ggcagattag tcggcgaagg aacccatgag gaattggtag ctggaaaccg
     8881 tgtctatcag gaaatctttg aaactcaaaa aggaacggag gagtaagtat gactgatcta
     8941 ataaaagcaa gtaaattttt ctatcactat ttgaagcggt acaaactctc ctttttcttt
     9001 atctttatca cagtgatcat tgctacctat ctacaagtaa aggcacctca atacatcggg
     9061 gaagcttttc aagaattagc taattacgta ggaggcttga tgcaaggcgt agacgataaa
     9121 agcaaatttg tttctgtaat atggaaactc ttgctgtttt atgtactagc tagtgcagcc
     9181 aactttatct atagtatttt gttcacacaa gtagtaggga agtcaacgaa ccgaatgcgt
     9241 atcggtctat tcaacaaatt ggaaaaattg acgatccgtt tctttgattc tcatcaagac
     9301 ggtgaaattt taagtcgttt taccagtgac ttagataata ttcaaaatag tttgaaccaa
     9361 gctttgcttc aagtcatcac gaatgctgtt ttacttgtcg ggatcttgat catgatgttc
     9421 cgccaaaatg tagagttagc ttgggctaca attgcttcca ctccagtagc cgttttgata
     9481 gctgtcttga ttatccgcaa agcacggaaa tatgtggatc ttcagcaaga tgaagtgggg
     9541 aaactaaatg gttatatgga tgaaaaaatc agcggacaac gtgtcatcat cacaaatggc
     9601 ttacaagaag aaacgattga aggattctta gagcataatc aaagtgttcg cgaagcaacc
     9661 ttcaaaggac aagcttattc tggtctacta tttccgatga tgcaaggaat gtcactggtc
     9721 aatacagcga tagttatctt tttcggcggc tggttagcat taaatggtga tttggaacgt
     9781 tcggtggcat taggcttagt tgtgacattc gtccagtatt cacaacaata ttaccaacca
     9841 ctgatgcaga tttcatctgg atacagcatg atccaattag cgattacagg ggcaagacga
     9901 ttgaatgaaa tatttgatga aaaagatgaa gtcaaaccag aaaacggtaa agcatttgat
     9961 ggtgttgaaa aaggtcttgc gttgcagcac gtagattttg gttattctcc agaaaaacta
    10021 gtcttgaaag atgtctcgat caatgtggac aaaggagaaa tggttgctct tgttggtcca
    10081 acaggatcgg ggaaaacgac gatcatgaat ctgctaaacc gtttttacga tgtcaatgat
    10141 ggttcagtag tcatcgatgg aatagatatc cgagattatg atcttgatgc attacgtaga
    10201 catgtaggaa tcgttttgca agaatctgta ttgttctccg gaacgatcaa agacaatatc
    10261 acattcggca aaccggacgc ttcagatgaa gaagtcatca atgcagcaaa acaagcgaat
    10321 attcatgact tcatcatgac gttagaaaaa ggttatgata cagagatatc cgaagaaaat
    10381 aacttattca gtacaggtca aaagcagttg atcagtattg ctcgaacgat catcacaaat
    10441 ccatctttat tgattttgga tgaagcaaca agtaatgtag acacggtaac agaagcaagg
    10501 atccaaaaag caatggatga agcaatcaaa ggaagaacca gctttgtgat tgctcaccgg
    10561 ctgaaaacaa tcttgaatgc agatagaatc gttgtcttgc gtgatgggga agtcattgaa
    10621 gaaggcagtc atcatgagtt attagcagaa aatggatttt atgcggaact ttatcataat
    10681 caatttgtat ttgaaagtta aattaaaaga acctcgttat aacggggttc tttttatata
    10741 ttttatgttg tttgttttta taaggggca
//
LOCUS       DAHHPA010000087        10504 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.088, whole genome shotgun sequence.
ACCESSION   DAHHPA010000087 DAHHPA010000000
VERSION     DAHHPA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.088"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(1182..1847)
                     /locus_tag="NBH55_002233"
     CDS             complement(1182..1847)
                     /locus_tag="NBH55_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510179.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNIRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2027..4009
                     /locus_tag="NBH55_002234"
     CDS             2027..4009
                     /locus_tag="NBH55_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCD1510180.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4013..4651
                     /locus_tag="NBH55_002235"
     CDS             4013..4651
                     /locus_tag="NBH55_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1510181.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4679..5107
                     /locus_tag="NBH55_002236"
     CDS             4679..5107
                     /locus_tag="NBH55_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510182.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5564..6475
                     /locus_tag="NBH55_002237"
     CDS             5564..6475
                     /locus_tag="NBH55_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCD1510183.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6929..7243
                     /locus_tag="NBH55_002238"
     CDS             6929..7243
                     /locus_tag="NBH55_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCD1510184.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            7259..7645
                     /locus_tag="NBH55_002239"
     CDS             7259..7645
                     /locus_tag="NBH55_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCD1510185.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            7674..9059
                     /locus_tag="NBH55_002240"
     CDS             7674..9059
                     /locus_tag="NBH55_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD1510186.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            9062..9214
                     /locus_tag="NBH55_002241"
     CDS             9062..9214
                     /locus_tag="NBH55_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1510187.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            9237..10442
                     /locus_tag="NBH55_002242"
     CDS             9237..10442
                     /locus_tag="NBH55_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCD1510188.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         3573 a         1705 c         2048 g         3178 t
ORIGIN      
        1 ctaatatatt catatgagct aattttatga ttaaatggta agtgtttcct tgtttgtttt
       61 agtttaaact agtgtgtaat ttaaaaatct atatggaagg gtctgtgggg taatacttaa
      121 ataaaggcta ggaacctgat aacatcaagg ttcctagcct ttttataata aaatgaagcg
      181 aaaataattt cttttaaaga gccgagatta tcagctatat aagagtgaat ttgatgcaaa
      241 aaacagagcc gaaacagtct tggtgttcgt agctctgctt atatgtttcg aatttaagac
      301 aaagagttgg caaaagatgt tgataaggaa gataagataa ctgtgttata taaactaggt
      361 aactaactgt aacaagctaa ttttaagaga ttcttattag aagacgttta ggaatataac
      421 taagaaaatg attatacagg taattgtcat ttcaataatg ttttctaatt ttcgcgtctt
      481 tgttttaaga aaaaggccag aatcagttgg aaaaaagttc gtacgatagt gaagaggggc
      541 aaaatttcct actaatattc ccactaacaa gagataaaac ggccaggttg gtgtgattcc
      601 tctataaaaa aatgcccatg ctaccgaaat cccatagatt aacaactgaa caatttttac
      661 aatgaaaaaa ttgttctgcc attactcaaa gtctcttcct ccttgtatcg tttatttgtt
      721 tcattttatc attatgatag gcgcttgaac aatccatttt gtattttatt gagtactctt
      781 ggataaacta ccagcgatta tcatatttat ttatgttact gtaagccatc atataaaagt
      841 aaatgagcag atcatagaaa tataaaaaag aactaaaatt gatctaaatt atgtgcagct
      901 tgactacgta ccaatctata ttacttacta gattggtcgt taatctattc ggatcgaatg
      961 agcaaaatat ttttgtgaat gtccatcctt gattatgatt ccaaaaagaa tcattgggag
     1021 gctgtggcgt aaatattata ctataagcca gaaataccta tcattctgaa cttttttatt
     1081 taggaacttt catctcaagg ctttttcaat taatttgtac ccctgttttt tggataagat
     1141 catgtgcatt gattttttct atctctacgt tcatctccta cttaatgcct atctggctcc
     1201 tgagggcttc tttttaaggc gtcgtcatag tcttggctga ggatttcttg gtaatctgag
     1261 ggaatggctt cccaagtgag caggcgaatg ttcatgacgt gttttccttt gtgtttgatt
     1321 acagcatggt ttttctcttt ttctacctct ccagcgtgaa aagacagcca tgtaccatgg
     1381 ccaaagatat cattacatac ccaacgttcg atggaattgt ccacgtatcc tacagggagt
     1441 gtggtatagc cgatttgtgg tttcaaaaaa ggattgaatc gatcgatcgt ttcatttttg
     1501 tattgataat tgataagaaa aagatagacc aaacaaaaga cgagcagaaa gaaagatagt
     1561 gtctttttga gtgagaaggg ttgttttgat gaataagatt tcttgttaac aagaggttca
     1621 tctggcaatg ttggttctat tgattcggta gctgattcaa tagaaggaaa atcaatcggc
     1681 agacctaaag tatgatgtaa aagtgacagc atttcaaggt ctggcattgt acgagaattt
     1741 tcccaactag aaatcgtttt atcggaaaca ccaatttttt cgcctaattc tttttgcgta
     1801 agcttacaat ttctgcggta agttcgtatt ttttctccgg tgttcatctt cttctccttt
     1861 cgattctaca gtatatagat ttattctaca tgacctatac acttgataga tacgtttttt
     1921 tgatgaaaaa cggtatgatt ttatcaaaga atcatttaag taagggagag tggtgataat
     1981 caaaaaaaag ttcacagttt tactcacaat catagtgacc ggtatcatga gtggttttgc
     2041 cttggttagt ataaataaac aggtactcta tcggacaaat gaatttttcc atcaaacaga
     2101 tacttatact ccgattcgat tgccacaaca ttttattggg caagaagaat atgagcagct
     2161 aatcatggta tttgaaaatg tctcatcaga aactgagctt tcttatatga aaaaaacagt
     2221 aaatgaatcc aatgaagtag atacatcttg gaacttcaag atacagccaa agcttgaaat
     2281 gacattgtat tatcaagggg atgtcaaatc ttctcacgag aaggtaccct attctaatac
     2341 ctcctttcgc gtaacgtcca ttgaagaatc gatcaaaata aaagattttg aaggtgaatt
     2401 tttcttgatg acgagagaaa aagaagccta tgaacggttc atttcactat tcactgatcg
     2461 attcaatcaa gcatttcaca ctgattatgc cactaatatt tttgaagact ttgaaaatag
     2521 ctattcgtat tcggttattt caaatacaga cgtatataat tatcgagtgt atttaactat
     2581 tggattgatt cttttattcc ttttgttatc atcctttttc ttattgatga ataaagaaat
     2641 cagtttattg ttgaataatg acttttcatt acatcaagta ttgtggtgtt tattggggaa
     2701 aaagaaaatc attattacat tcataattgc aggaatcatt ggtggtttta tttttatgta
     2761 ttataaggag caatttcttt atttgttcca gatgtacgtt ttcttgttca gcggaatata
     2821 tggtatctct ttgatttctc ttttattagt tgaaaaaatc gcttcgttaa gagaagcgtt
     2881 caactatatc caatacattc tttttacatt tggattgttg ttttctcttc ttttgaccgt
     2941 tgccaatgtc gaattagcaa ctatcgtttt ttcttcttca cagttagctg tttcccatga
     3001 accaccaaaa gaaatcgctg gtgaggacta tcatgtattt tatccagtaa ccatcgggaa
     3061 aaaccacgtg gattttattt attccaatcg ttttgcatca gcagcagatc aggaattata
     3121 tgaaactttg aataaaaacg gaagtatatt agtcaatgta tcagattact tgaatgaaga
     3181 aaatgaacag tttggcagag ggataaaaat aaatctgaat tatcttaaga aatttccttt
     3241 gattgatgtc tctggaaggt tgatccaaat aaccgaaaaa gaaacgcacc ctgttatatt
     3301 gatccctgaa cgatatagac atacagattt aaaaaatgac ctggcgcaaa taacagaata
     3361 ttatcaagaa atcagtgaaa aagaaccgaa atttctattt ataaaaaaca atcaacccat
     3421 ttatacattt attccttcta tcccctggat cgaacattat ccggttgttg aaatactgac
     3481 attgaaaaac agtatgtatt tggaaagaaa tattctcagc ggagaaatct atccaccgtt
     3541 aaagatcaag ataggctctt tgtcgaaaga aaggttattt gagctgattg aagaaagtca
     3601 gttacgtgat aacttgcagt tatcatttcc gtatactcag actgaagaga tagctgtcaa
     3661 agtgttaagc agatcatttc attacttgat acagatattt ttactcactt tcttttcttt
     3721 cttcttgttg agctatgtca tgttatgcat atattttgtc tacaattgtc ggaaaatcgc
     3781 tatctttcgt tcaagtggtt attcgctctt tgaaacgtat aaagatttct tcatgatgaa
     3841 cctcatcaaa tggggaacga caagtgtaat cttccttttt cttatagaaa gagagcctaa
     3901 atatcttttt aatatttttt tctttagctt cattgatttt attctttcag ttgtttttat
     3961 tttgtctaca gaaaagaaaa gtcagttatt gctgatgaat ggaggttaac aattggagcc
     4021 gttgatagtg ataaaaggtg cgaaaaaaat cattcgaaac aaaacgattc ttcatttgga
     4081 tcgtttcact gcggatgctg gggatcttgt gactgttgtt ggagaaagtg gagcaggtaa
     4141 aagtacgtta ttgaatatat taggattgaa tgatcagttc agttcaggac aattctattt
     4201 gttcgatcaa gagacgaaac aattaactgg gagagagaag cgcttgataa aaagaaatac
     4261 catatctttt ctttttcaag attttgggtt gattgaatat gaaacaatcg attttaattt
     4321 agatattgga ttgaagtttt cgaatttaaa tgtttcagat aagcgaacag caaagtatga
     4381 ggcattgaaa aaagtaaata taaacaagaa gctaagtacg cctatctaca cattatctgg
     4441 tggtgagaag caaagaatcg cctgtgcgag agtcttattg aagaatagca aaatcatttt
     4501 agccgatgaa ccgactgggt cattggataa aagaaataag gataaagtag ttgaaatctt
     4561 atcgcaacaa gcttcccaag gtacgttagt tatcgtagtc acgcatgatc catatctgac
     4621 caaaatagga aacaagaaga tcatgcttta aaaatcaaaa aggaaaggat gtcgttttat
     4681 gaaaaaagca gttattattt gtgcactagg gataggtttt gttggaggaa acgctatgtc
     4741 atctgaagtg gaagcaaatt cagttcacgg tgaaatctat tacaaatctt taaagccagg
     4801 agagattcat tttgatgcta aaggtaatat tattaagtca aatttaccag tagaccggga
     4861 aatttctaga acagcaatta atcatagctc tggaagatgg gtttattata gtgatacaac
     4921 ttgtacgaga aaaacaggtc attctaacca ctatagtcgt gtctataatc gtcacggtgc
     4981 aaaagcgaaa gttggaaact ctattcgatc agcaaatgca acaaaaggag tatgggccta
     5041 tgcaagtgcg acaggaaata gaactgacac attctcatgt tggtacaatc caaccggttg
     5101 gtattaagta tccaaaagtt tatataaaat agaaaaaaca tataatgaaa cagcgttaaa
     5161 agttattttt ggtagtttta atatattcat ttttagttgt ataaaaatat atcataataa
     5221 aaacaacaaa aaaatgtctg gaaaatcgaa cataaaagtg tctgatttga atatcccagc
     5281 agcggaagaa gaaaaacaaa atcgataaca tttgttctaa gaattagtta cgttaaatac
     5341 ggaacgcaag atagacttga aaaacggtaa caaaagaagt agtatattaa ggtgtgatgt
     5401 gctacttgac atttattctg ctgagcgaac tttctggatt gtgtagcctt atatctattc
     5461 aggacgtatc gagcagatta gtaaaggtgt tcttgatgat tttcatcttg aacacctttg
     5521 tttttagcaa ttcgcaaaaa acgaaaagta ggagaattga taaatgaaaa aaagattttt
     5581 agcacttgcc atcgtactag gcactggctt attgtcaggc tgtacgaatg ccggtgaaaa
     5641 aacagccgtt agttataaag gcggtacaat cagcgaacaa gaagtaatgg atagcttgaa
     5701 aaaaatgcaa ggagcagatt ccgctgtgca acagttgatc gtttatcaag tatttgaaga
     5761 taaatatgga gatgacgtat caaccaaaga aatcgattct caatatgatc aaacaaagaa
     5821 acaattgggt gattcatttg attcacaatt gaaatctgca ggttacacag aacaaacatt
     5881 caaagacagt atcaaacaaa gtctagcctt tcaagaaggt ctaaagaaac atatcaaatt
     5941 gaccgatgaa gatttaaaaa cggcttggga atctttccat ccagaagtgg aagctcaaat
     6001 tatccaagta gcaagtgaag atgatgcaaa agatgtgaag aaagctgctg ataagggaga
     6061 cgatttctct aaattagcca aagacaagtc aactgatact acgacaaaag aagatggcgg
     6121 aaaagtgaaa tttgattcca ctactacaac ggttccggca gaagtcaaag aagcagcctt
     6181 taagttgaaa gatggtcaag tttctgatgt gatcacttct accaatgctt caacttatac
     6241 tacggaatat tatgtggtta aaatggtgaa aaaccaaaac aaaggcaacg acatggataa
     6301 atacaaaaaa gaattgaaag aaattgcaac agacacaaaa ttaagtgaca gtaccttcca
     6361 gaataaagta attggcgaag tattgaaaga cgctaatgta aaaatcaaag acaaagactt
     6421 tgaaaatgtt ttgagtacat tcacttcaga ttctagtaca gcaagtagta aataagcggt
     6481 ataaataaaa aatatgaact atttaaatca ataaaactgt tcatctaaat ctttttatta
     6541 gaactaaatc aacagtagtt taattactac ttgatttagt tctttttcta caaaaaatag
     6601 cataaaaatc tagttatccg cataaaaact ggacttatca cactttatca aggtcaaaac
     6661 cactcaattt actactaatt tactacttat gaatgagctt tgatacgacg atttatcctt
     6721 gaaaagtgaa gatataaaga tacttccaat aaaatttgaa tatttaatag gtagacactt
     6781 caaaaaatga ggtgtctatt tttttacccg attttgaaag gaagtgaact tatgaaaaca
     6841 aaaaatcaag aatcaaaagg tcgttcccca ctctttaaga ccatcaaaca ttcattcagc
     6901 caataaaaaa gaaaggatag gtaaaaatat ggaacttaaa tttgtgattc ccaacatgga
     6961 aaaaacattc ggcaatttag aatttgctgg cgaggataaa gtcgttcagc gaagaatcaa
     7021 cggacggcta actgtcttat caagaagcta taatctctat tctgatgttc aaagagcaga
     7081 tgatattgtg gtggtgcttc ctgctgaagc tggcgaaaaa catttcggct ttgaggaacg
     7141 tgtgaagtta gtcaatccac gtattaccgc agagggctac aaaatcggca ctcgtggttt
     7201 tacaaattac cttttacatg ctgacgacat gataaaagaa taaagaaaga gaggaaaaat
     7261 gatgagatta gcaaatggca ttgtattaga taaagacacg acttttggag aattgaaatt
     7321 ctctgctcta cgtcgtgaag tgagaatcca aaatgaagac gggtcggttt cagatgaaat
     7381 caaggaacgt acctatgact taaaatccaa aggacaagga cgcatgattc aagtaagtat
     7441 tcctgccagc gtgcctttga aagagtttga ttataacgca cgggtggaac ttatcaatcc
     7501 cattgcggac accgttgcta ctgccaccta tcaaggagca gatgttgact ggtatatcaa
     7561 ggcagacgat attgtgctga caaaggattc tagttcattc aaagctcaac cacaagcaaa
     7621 gaaagaaccg acacaagaca aatagtcgct aggtagaaag gagacttttt cgcatgaaac
     7681 agcgtggtaa aaggattcgc ccatctggta aagatttagt ctttcatttt acgatagcgt
     7741 cactcctgcc tgttttcctg ctggttgtcg gactgtttca tgtgaagaca atccagcaga
     7801 tcaactggca ggattttaac ctatcacaag cagataagat tgacattccc tatttaatta
     7861 tcagtttcag tgtcgcaatt cttatctgct tgctggtagc gtttgtattc aaacgggttc
     7921 gctatgatac ggttaaacaa ctttaccacc gtcaaaaact ggcaaagatg atacttgaaa
     7981 acaagtggta tgaatctgaa caggtcaaaa cagagggttt ctttaaagat agtgctggtc
     8041 gtacaaagga aaagataacc tacttcccta aaatgtatta tcgacttaaa aatggcttga
     8101 tacagatacg ggtggaaatc acgctgggaa aatatcaaga ccaactctta cacttggaaa
     8161 agaaattaga gagtggcttg tactgtgagc tgacggataa agagttaaag gattcctatg
     8221 tggaatatac tttgctctat gacaccatag ccagtcgtat ttctattgat gaagtagaag
     8281 ctaaagatgg taaacttcgc ttaatgaaaa acgtatggtg ggaatatgat aagctccctc
     8341 atatgttgat tgctggtggt acaggtggcg gtaaaactta ctttatactg acactgattg
     8401 aagccttgct tcatacagat tcaaaactgt atattcttga cccgaaaaat gctgaccttg
     8461 cggacttagg ttctgtgatg gcaaatgtct actatagaaa agaagacttg ctttcttgca
     8521 ttgaaacatt ctatgaagaa atgatgaaac gtagtgagga aatgaagcag atgaagaact
     8581 ataagactgg caaaaattat gcttacttag gtctcccggc acacttctta atctttgatg
     8641 aatacgtcgc tttcatggaa atgctgggaa caaaagaaaa caccgcagtt atgaataagc
     8701 tgaaacagat tgtcatgtta ggtcgtcaag ctggcttctt tctaatactg gcttgtcaac
     8761 gtccagacgc aaaatattta ggcgacggaa tccgtgatca gtttaatttc agagtggctt
     8821 taggtcgtat gtctgaaatg ggctatggca tgatgtttgg cagtgacgta caaaaggatt
     8881 tcttcttaaa gcgaatcaaa ggtcgtggct atgttgatgt aggaacaagt gtcatatcag
     8941 agttttatac tccccttgta ccaaaaggat atgatttctt ggaggaaatt aaaaagttat
     9001 ccaacagcag acagtccacg caggcgacgt gcgaagcgga agtcgcaggt gtggactgat
     9061 cttgctggct ggtgtggcaa tagccacgcc agcacttaac cccccgtatc taacaggggg
     9121 gtacaaatcg acaggaaaca gtcaaaaaaa cattagaaaa tcctttggtt acaagggatt
     9181 tacaaaattt cagcgtatgt caaatgggct ttaaaagttg acatacgcct ttttgattgg
     9241 agggattttt actgaatgaa caaacttggt tacagcattt aaaagaaaaa cgcttggctt
     9301 atggactatc tcaaaaccgt ttagctgttg cgactggtat tacaaggcag tatctaagcg
     9361 atattgaaac aggaaaagtc aagccatcag aggatttaca gcagtccctt tgggaagctc
     9421 tggaacgctt caatcccgac gctccccttg aaatgctgtt tgattatgta aggattcgct
     9481 ttccgacaac agacgtacag caggtggtcg aaaacatctt acaactgaaa ctgtcctatt
     9541 ttcttcatga ggactatggt ttctattctt attcagagca ttatgcttta ggcgacatat
     9601 tcgtcctttg ctcccatgaa ctggacaaag gagttctggt ggaattgaaa ggtcgtgggt
     9661 gcagacaatt tgaaagctat cttctggcac aacaaagaag ctggtatgag ttctttatgg
     9721 acgttttggt ggctggcggt gtgatgaaac gccttgacct tgccattaac gataagacag
     9781 ggattttgaa tatccctgta ctcactgaaa agtgccaaca ggaagaatgt atctccgtct
     9841 tccgcagttt taaaagctat cgcagtggcg aactggtacg caaagaggaa aaggaatgta
     9901 tgggaaacac cctctatatc ggttcattac aaagtgaagt ttatttctgt atctatgaaa
     9961 aggactacga gcagtacaag aaaaatgata ttcccattga agacgcagaa gtaaaaaacc
    10021 gttttgagat tcgattgaaa aatgagcgtg cctattatgc agtccgtgat ttactcgtct
    10081 atgacaatcc agagcatacc gcctttaaaa ttatcaatcg gtatatccgt tttgtagata
    10141 aagacgattc caaacctcgt tctgattgga aactgaatga agaatgggct tggtttattg
    10201 ggaacaatcg tgaacgatta aaactaacca caaaaccaga gccttactcc ttccaaagga
    10261 cgctgaactg gctatctcat caagttgccc cgaccttaaa ggttgcgatt aaacttgatg
    10321 aaatcaacca gacgcaggtt gtaaaagaca ttctcgacca tgcgaaactg acagaccgac
    10381 acaagcagat tttgaagcaa cagtcagtaa aagaacagga cgtgataaca acaaaaaaat
    10441 aactcaaata caaattcatt gaatatagag aggagaacat ttttatgaat tttggacaaa
    10501 acct
//
LOCUS       DAHHPA010000088        10470 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.089, whole genome shotgun sequence.
ACCESSION   DAHHPA010000088 DAHHPA010000000
VERSION     DAHHPA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.089"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            23..646
                     /locus_tag="NBH55_002243"
     CDS             23..646
                     /locus_tag="NBH55_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510189.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1095..2144
                     /locus_tag="NBH55_002244"
     CDS             1095..2144
                     /locus_tag="NBH55_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1510190.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2141..3022
                     /locus_tag="NBH55_002245"
     CDS             2141..3022
                     /locus_tag="NBH55_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1510191.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3025..3795
                     /locus_tag="NBH55_002246"
     CDS             3025..3795
                     /locus_tag="NBH55_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1510192.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4074..4928
                     /gene="aroE"
                     /locus_tag="NBH55_002247"
     CDS             4074..4928
                     /gene="aroE"
                     /locus_tag="NBH55_002247"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCD1510193.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5106..6269
                     /locus_tag="NBH55_002248"
     CDS             5106..6269
                     /locus_tag="NBH55_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD1510194.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6386..6796
                     /locus_tag="NBH55_002249"
     CDS             6386..6796
                     /locus_tag="NBH55_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1510195.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6970..9198
                     /gene="topB"
                     /locus_tag="NBH55_002250"
     CDS             6970..9198
                     /gene="topB"
                     /locus_tag="NBH55_002250"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCD1510196.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9369..9653
                     /locus_tag="NBH55_002251"
     CDS             9369..9653
                     /locus_tag="NBH55_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510197.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9813..10049
                     /locus_tag="NBH55_002252"
     CDS             9813..10049
                     /locus_tag="NBH55_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD1510198.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10046..10444
                     /locus_tag="NBH55_002253"
     CDS             10046..10444
                     /locus_tag="NBH55_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCD1510199.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3647 a         1762 c         2222 g         2839 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aattggcgca gctggcgtag ataagttgta ttaatggtga gactttccca
      901 tagatacaac tttttagagt gcatttctaa agcttgtatc tatgctagac atgaacattg
      961 actgccgcat agattagaat ctatgcggtt ttttgtgtaa agacaatgac cgctttggcc
     1021 attgtctttt tttgtagact ggatacttgc ctgattataa taaataaaaa gattgacttt
     1081 gcaggaggaa aaaaatgaaa aacaaacgat taataacagt tgtagtaatt attttagttt
     1141 atttagcagg tgcatttatt tatggaaaag cctcaggcga aaaagaaagt acttcgacga
     1201 accagacaaa aaaaacaaca gttgttgggg tattgcaata cgtgagccat ccggcattag
     1261 atgagattta tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa gaaggagaaa
     1321 atcttgagat caaatttcaa aatggacaag ccgatcagag caagctagcc acaatgagtc
     1381 aacagctagt acaatctgat ccagatgcct tgattggaat cgcaacacct gccgctcaat
     1441 cattagcaaa cacgacttca tcgatcccag ttgtgttagg tgctgtaacg gatcctgtgg
     1501 gggctggatt agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt gtttctgatc
     1561 agccgcctgt tacttcacaa atcaagcttg cttctgattt gttgccaaat gcaaaaaaag
     1621 tcggaatctt gtatgcttct tcagaggata attcaaaata ccaagtagca caagcagaag
     1681 aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc actaatgaga
     1741 ttgcacaaac ggttcaagtg atgagccgac aagtcgattt tatctatgtt ccattagata
     1801 ataccattgc caatgcgatg caaacagtgg taaaagaagc aaacaaagca aatatccccg
     1861 tcattccatc tgtggataca atggtggaac aaggtggact agcaacaatc gggatcaatc
     1921 aatatcaatt agggcttcaa agcggtaaga tggcggcaga acttgcgagt ggaaaagaaa
     1981 aaccggaaac gacgcctgtt tatacatttg atcaagggga tacagtgatc aatcaatctc
     2041 aagctgatcg cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc aaaatcatta
     2101 cagatgactc gcagcaagaa acatctaagg aggatgacaa atgatcgtat cagctatttc
     2161 acaaggcatg ctttgggcaa tattaggatt agggattttc atgacttatc ggatcttaga
     2221 ctttcctgat atgacgacag aagggtcctt cccattaggt gctgctgtct gtgtgacagc
     2281 tattactcat ggggtagcgc cgattgtagc tacattatta ggcgtaggtg ctggcatgat
     2341 agcaggactc gttacggggt tactttatac aaaaggaaag ataccggtta ttttagcagg
     2401 gattttagtg atgtcaggat tgaactcagt tattttgttt gtgatgcgaa caccaaatct
     2461 ttctttattg aatcatccga ttctacaaga cagcttccat cgctttgggt tacctgatta
     2521 ctttgacaca gtccttcttg gcttcatctc tttgggcatc ttgattgctg tactgctatt
     2581 cttcttcaat acagatttgg gtcaaggcta tattgcgaca ggagacaacg aaacgatggc
     2641 tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta ggtttaactt tgtcgaacgg
     2701 tgtcattgca ttatccggtg cgctgattgc ccagaacgac ggatatgcag atgtaaataa
     2761 agggattggc gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag tgatcttcca
     2821 agaattgacg ctagcagaaa gattgattgc tattgttgtc ggcagtatca tttatcagct
     2881 attgatttta gtagtaatca aactagggtt tgacacgact tatctgaagt tgttctccgc
     2941 tatcatctta gctatttgtc tgatgatccc gcaattgaag aaagcactga acttaaaaac
     3001 gggttttgag aaggaggcat agtaatgact gatatattga caatcaaaaa tggcagaaaa
     3061 gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg atgaaattaa ccttacaatc
     3121 agagaagggg acttcatcac tgtccttggc gggaatggtg ctggaaaaag cacactgttc
     3181 aatacgatcg ctggtacgct tcaactcagt caaggatcga tctcttttaa agatcagctg
     3241 atcacaaaag attcagaaga aaaaagagca cgattccttt ctcgagtttt ccaagacccc
     3301 aagatgggaa cggctcctcg gatgacagta gcagaaaatt tgctacttgc acaaaaaaga
     3361 ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga ttttttcaga
     3421 ctatgtcaag aagtcggtaa tggcctagag caacacttgg atactccagc gggtgaatta
     3481 tctggtggtc aacgccaagc attaagcttg ctgatggcga cgatcacagc tcctgagtta
     3541 ctattattag atgaacatac tgcagcttta gatcctaaaa cagctaaagc attaatgcga
     3601 ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac gcatcgaatg
     3661 gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa aatcgttcaa
     3721 gatatttctg aaaaagaaaa gcaacatttg acaatgggcg accttttgca gttctttgaa
     3781 gaagtagtgg attgatttcg acagaaaaat tcctttcagt agaaggactt gaaaactcat
     3841 tgacatcttc ctttgattca tcctatactt aaaacaataa acaattaaac atatttgggt
     3901 gttttgtagg atcacgtagt caatgatttt aacaattaaa tagaatattc tttgctctga
     3961 caagtaaacg atgagaaaag aatttgctac tttgccacct agacgtttcg tactttttcg
     4021 tatcaaacgc taagtggctt tttttattga agtaagaaca aaggaggaat aagatgattt
     4081 ctggacaaac caaattagct gggctctttg ctgatccggc ttctcacagt ttatccccat
     4141 tgatgcacaa tactgctttt gaagcaaaca acatcgatgc ggtgtattta gctttccaag
     4201 taaatccaat gaatttgaag caagcagtcg aaagtattcg aacttttgat atgttaggtg
     4261 taaatctttc gatgccgaat aaaacagcgg ttattcctta tctggatgag gtaagccagg
     4321 aagcgcaatt gatcggagca gtgaatacga tcgtgcacga aaagggtcgt ttgattggct
     4381 ataatactga tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt tcgatcaaaa
     4441 agaaaaaaat gactctactg ggagctggag gtgcagcaaa ggcagtcact gttcaagcag
     4501 cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca ttcgctcaag
     4561 taaaagaaac attcgaacaa ctcagctatc gaacttcttg taagataaaa gttcgtgact
     4621 ttgccgatac gcatcaatta agaaaagact tgaaagaaag ctgtcttcta gtcaatgcaa
     4681 cagatatcgg gatgggaaag aaaatcaatc agtcaccgct cttagatata agcatgatgc
     4741 atgagaatct agcggttttt gatttgattt atgcacctag agaaacacgt ttattaaaag
     4801 aagcaaaaaa agtaggagca aaaggataca atggacttgg catgttgatc taccaaggag
     4861 caatcgcgtt tgaattatgg acaaaggaaa agatgccgat ccatgtgatt gaaaagctgt
     4921 ttgattaaaa aaggataata gataaagatc ttttattctc gcataaacgg tctcaagaaa
     4981 agtaatcctt tatgtatcga agcatttatt tcgttatttt ttattaaatt atcaaaaaat
     5041 gataggcttt tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa cgaggaggaa
     5101 ccacaatgag tcggatggat cgctataagg acatccataa gaaggcgaaa cctctgaagc
     5161 gggatacggg gagtattttt gctagaagag aacgaaaacg aacgcgtatg gaaaatgagg
     5221 aagatgctac ccgtatttat gaaagagagc agcattcatt tgaacatttg aaccaatacg
     5281 agcaacagat atacagagat gcaccaagag cagaagaaca acaaaaaaat aaaaaatttt
     5341 tcaatcggca aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg tggaaaaaaa
     5401 tcattctatc gattttgctt gttttgatcc tttattcagg tatctcattt tttattggta
     5461 aatatgtagc tgaacatgac acaagcttag aggcagcgac ttcagaaacc tttcatgggc
     5521 aaaaagcgac gaatggtgcg cataatattt tgatccttgg aagtgataca cgcggagaag
     5581 atgccgggcg agccgatacg atcatggtct tacagttgga tggtccttcc cataagccga
     5641 aactgatttc ttttatgcgt gacagcttcg ttacgatccc aggggtagga caaaataaga
     5701 tcaattcagc ttatgcatac ggaggagccg atctagtgcg tcaaacttta gtagaaaatt
     5761 ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa aaagtgatcg
     5821 atgccttatt catgaacgga gtgaagatcg atgcagaaaa agatctgaat ctggatggtg
     5881 tagatattaa aaaagggggc cagaagatgg atggacatgt tcttttgcag tacgcacgtt
     5941 tccgaatgga tgaacaagga gactttggcc gtgtcagacg ccaacagcaa gtaatgaacg
     6001 ctattttcag ccaattgaag aatccattga atctgatccg tgcaccttat gcagctggta
     6061 aagctttagg atatacatcc acagatgtca ctgcttggtt cttgacgaaa aacctctttt
     6121 cgattgcacg aggagctggt ggcattgatc gaataagtgt gccagtagac ggttcgtgga
     6181 attatgggca aagtgattat gctggcagta ttcttgaaat tgataatcaa atgaatcaat
     6241 ctgctattac agattttttg aataaatgat cataaaaaag atcaagaaga agctgcgcaa
     6301 ggcttttgtc atgaatcaat caaacgattc actcgtaaaa aacgctttct tatggtagat
     6361 ttaaccaaga gaggagctgt ttacattgga tatctatctg aacttcaaaa atgagtcgcg
     6421 tgaagccatc gctttttatg aaaaagtttt tcagactgaa gcatcaggta tcaccacata
     6481 cggtgaaatg cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa atctaatcac
     6541 gaatgcacgg ctgaacattt atgatacatt agttatgttt gcagatacgc cagaaggaat
     6601 tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc atccagacaa gaaatgaaga
     6661 agaaacgaag cgcctatttg aaagcttagc tgagggtggt aagattgaga tgccactgga
     6721 tgctgttccg tgggctcagt tgtatgggat ggtaactgat cgatttggca tcaactggca
     6781 gattaaccaa gagtgacagc taaagatgaa tattttctgg aaacatcaag cagcaaacgg
     6841 atcatcgttt gtttgcttga tgtttttgtg tctatttcga ctatacggta tctttttttt
     6901 attgccagat gcgaaaatat gttccataat agagaagtcc gcaaaaaatt aagtaacgga
     6961 ggtcagaaga tgaagacggt gatactagct gaaaaacctt cacaagcaaa agcctatgcc
     7021 gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg aaatcagcga tccattgttt
     7081 gctggtgaag taaccatcac ttatggtttt ggacatttgg tagatatggt tgctccagga
     7141 gcttatgaag agcgttgggc aaaatggtct ttagaaaatt tgcctatttt cccagaaacc
     7201 ttccgttacg aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa acgagaattg
     7261 cagtcagccg ataccatcat tattgcaaca gatggtgatc gagaaggtga agccatcgcg
     7321 tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa agaactattt acgtttatgg
     7381 atcaattctc ttgaaaaaga agcaatctat gaaggtttta agaatctcca acctggaaag
     7441 aactattttc caaaatataa agaagcgcaa gcaaggcaga acgctgattg gctgattgga
     7501 atgaatggaa gtccattata tagcttgcta cttcaacgaa aagggattga tggcagtttt
     7561 tctcttggtc gtgtacaaac accgacactc tatatgattt atcagctgca agaagaaatc
     7621 aaacacttca aaaaagaaac gtactttgaa ggagaaggtt taattacgac aaacaaaggg
     7681 cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga gttaacgaag
     7741 gaaatagaaa aacttggagc gcatttaggt aagcaaaacg gacggattct tgaagttacg
     7801 aaaaaggaaa agaggttgca tagtccacgt ttattttcgc tatctagttt gcagacaaag
     7861 atgaatcagc taatgaaagc aagtgccaaa gctacacttg atgctgctca aggattgtac
     7921 gaaagcaagt ttctaagcta tccaagaacg gattcctttt acattacaga aaatgagcat
     7981 cagtatctgg ttaaacatct gcaaaattat aaaaaatttt taggaataga agaagtacag
     8041 acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca agaacatcat
     8101 gcaattattc cgacaaagac gattccgact cctgcacgct ttgcgaagct ttccaaatta
     8161 cagcaagcaa tctatttgca agttttgcgc actactgtgg ctatgtttgc tgcagattat
     8221 atctacgaag agacgaccgt aatgacgggc gtacagcaat taaagctaaa aagcatcgga
     8281 aagatcccat tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa aaagaaagaa
     8341 gctcagactc ttcctcctat cgtagaaaat gaagaagcag aagtagaact gaaaagtatg
     8401 gagaaagaaa cacaacctcc aaaaccctat accgaaggaa cgttgatcac agcaatgaaa
     8461 actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga agtagaagga
     8521 atagggacag aagctacacg agccaatatc attgaaaatc ttaagcagaa gcattacatc
     8581 gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa tcactctttg taaggcagta
     8641 gccaatgaac cattactgac gagcgcagaa atgactgcgc gctgggaagg gtacttgaga
     8701 aaaattggta aacaagaggg gacaccatct gtttttttag agaatatcaa aaaattcatc
     8761 ctacatttat tggcagaagt tcctaatcaa atcgacaagg tagacttttc agatgagatt
     8821 agcgggacac gtcagataaa agcgatggag aagaaaaacg atcaattagg aagatgtcca
     8881 aaatgtaaaa agggaatagt catgctttat cctaaaatcg ctacatgctt gaatccggaa
     8941 tgcgacttca aattgtggcc aacagtagct aaaaagaagc tgactaaaac gaatctcaga
     9001 gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg taaaaaaggg
     9061 aaattcgatg cagttttagt attaaaagaa gatatgacaa tcggtttttc ttttccatgg
     9121 atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag aaacatcaag aaaaaataat
     9181 gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga atatttattc cgaaaagtga
     9241 gaatataaat accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac gttggggtag
     9301 gctgatttta tatagatagc aaggagtttt tttatttgaa aaaagtgcat tcatttttaa
     9361 attatcttat gggagcaatc acgattgtgg ctgtgtacca agcatttcta aagtatcctg
     9421 attacgggac gaagttgttg tcattgacgc acaatcaaac agcatattca ccaacgttga
     9481 ttttatcgac tttgactgca ggagctaaaa gcgctactta tttttttcat aaaacgacaa
     9541 gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg gctatgatca
     9601 ttatttattt tctaccagtt attacacaag tatttcatca atattttggt taagaatacg
     9661 agagtttgtg acaaaagtca tgtcacaaac ttttttcttt gtcatttttt cgtaagaaaa
     9721 agcaaaataa aagtatatac attgattaaa agtatatact tggatataat gtacttacta
     9781 tagaatgaga atggtataga gggtggcgaa atatgaaaaa aatgaaaaag tataaaatta
     9841 gaaaatctgg caatagcgat gtaacaacta tcccaccaga agtaaaagaa ttcatgggtg
     9901 tgcagacagg tgacgcaatc agttatgttt ttcaatctga tggttctgta cgaatgatca
     9961 aagcacaaga agaacctgat attgattcat tagttgattc gatcatgaat caatatgaag
    10021 acgcgttaaa agatttggtt gatttatgag gtatctttcg actaaagaaa ttatcaaaat
    10081 caacgcgaaa gttattttac gtcactcgcc aggcgaaatg atcggtgtaa aagatgcaaa
    10141 tgcattagat atggccgtca aacagccttc acaagcagta ttcaatcaag aactctatcc
    10201 tgaaatctat gaaaaagcgg cgattttagc aattaattta gctaaaaaac atccttttca
    10261 taatggcaat aaacgaactg ctcttgttgc catgttgttg tttcttcaat tgaataattg
    10321 tccagttgct ttttctcgtc aagaagctgt tgattttatc ataatgatta ccactagttc
    10381 attggatttc gattcactaa agtccaagat aaccagttat ttacgacaaa ccgcaataaa
    10441 atgaacgaat taaaaacttt atttctcaat
//
LOCUS       DAHHPA010000089        10284 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.090, whole genome shotgun sequence.
ACCESSION   DAHHPA010000089 DAHHPA010000000
VERSION     DAHHPA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.090"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            189..338
                     /locus_tag="NBH55_002254"
     CDS             189..338
                     /locus_tag="NBH55_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCD1510200.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            349..1863
                     /gene="dltA"
                     /locus_tag="NBH55_002255"
     CDS             349..1863
                     /gene="dltA"
                     /locus_tag="NBH55_002255"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCD1510201.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1860..3065
                     /gene="dltB"
                     /locus_tag="NBH55_002256"
     CDS             1860..3065
                     /gene="dltB"
                     /locus_tag="NBH55_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCD1510202.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3110..3343
                     /gene="dltC"
                     /locus_tag="NBH55_002257"
     CDS             3110..3343
                     /gene="dltC"
                     /locus_tag="NBH55_002257"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCD1510203.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3346..4614
                     /gene="dltD"
                     /locus_tag="NBH55_002258"
     CDS             3346..4614
                     /gene="dltD"
                     /locus_tag="NBH55_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCD1510204.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4755..5837
                     /locus_tag="NBH55_002259"
     CDS             4755..5837
                     /locus_tag="NBH55_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD1510205.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMGT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5837..6640
                     /locus_tag="NBH55_002260"
     CDS             5837..6640
                     /locus_tag="NBH55_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCD1510206.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6710..7450
                     /locus_tag="NBH55_002261"
     CDS             6710..7450
                     /locus_tag="NBH55_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1510207.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7461..8465
                     /locus_tag="NBH55_002262"
     CDS             7461..8465
                     /locus_tag="NBH55_002262"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCD1510208.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8510..10207)
                     /locus_tag="NBH55_002263"
     CDS             complement(8510..10207)
                     /locus_tag="NBH55_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510209.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3305 a         1698 c         2083 g         3198 t
ORIGIN      
        1 ctttattttt tatatttttt aaaagtacga gaaattataa agaaaaaagc agtgatttga
       61 tgtgctattt ttataaaagc tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag
      121 ctagacacat gtttcttttt gtcaaaatta gttacacttt gtgtgaggtt atatgaggag
      181 ggaatccaat gaacaaatgg cgttctaatc caaaattgaa ttattgggga aaattcatca
      241 ttcgaacaat tttttatagt gcagtactgc tgttcctgat ttatctgtat cactataaaa
      301 atattcaagg aggaactttt atctataacg aattttagga ggaagagtat ggaaatcaaa
      361 acgattattg aagcaatcga tgattgggga ataaatgaac ctgatcgagt tgcttatcag
      421 gcggatgaga cgcatacttt tggtgaattg aaacaagcat cagacgcact tgcttattat
      481 ttagaaaaaa aggtagaagg cgatggacca attgtcgttt ttggcaattt ggagtttgaa
      541 atgattgtct catttctagg ggttgtcaaa tctggccatg cctatttgcc gattgaagag
      601 catacaccca aggagcgtat cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc
      661 agtatcgggg actggataga agatattcct tcggttcctg tcataacaaa aaaagaattt
      721 caagaaatca agaagatacc agtaggattt tatcccagaa atagcgtaaa aggtacagaa
      781 aatttttata ttatttttac ttctggtact actggagaac caaaaggcgt acaaatcagt
      841 catgataatc tagtcagctt tgttcaatgg acgatggaag actttgggat caagcctggt
      901 atgcatttct tagcccaagc gccattttct tttgatttat cggtattttc tatttatcct
      961 gcattagttt ctggcggaat gctgaagccg ttaaataaag cagttgttca agactttcgt
     1021 caattattcg cgacattgcc agccttgaag ttgaatgtgt gggtttctac tccatcattt
     1081 atggatattt gcttgatgga acctacattc aatggagaaa atgtgccgga attggacatg
     1141 tttttatttt gcggagaaga attgacgaaa aaaactgcag aaagtctact tgatcgattc
     1201 ccaaatgcac ggatatataa tacttacggt ccaacagaag caactgtagc tatttcaagt
     1261 attcagattg accagcatgt actagatagt tatgaccgac taccgattgg ctacgtaaaa
     1321 aaagacacga aagtttctat tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt
     1381 gaaatcatta ttgcaggacc aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca
     1441 gctgctgcgt tcttttgtca aaatgactta gcaagttacc atacagggga tgcaggtaga
     1501 ctagatgaat atgggctttt attttatgaa ggacgtatgg atcagcaggt caaattacat
     1561 ggataccgga ttgaactcgg ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa
     1621 caagctgtcg tcgtaccaaa ataccaagga acaaaagtac agcagctggt tgcttttgtc
     1681 gtattagaaa acaaaacgaa acagccggat tttcagttga ctaaatctat caaagaacaa
     1741 ttactagaag ttgtaatgga ttatatgatt cctcagaagt tccattatgt cgatctgctg
     1801 cctcagacag ttaatggaaa aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa
     1861 tgatgttacc tcatatgatc ccttatgcag agccgtttta tttttttctt ttagccattg
     1921 cgcttgtacc gatcgttcta tctttattga tatgggaaaa aagattacca gtttatcaat
     1981 cagcagtgac acttttcttt ttatttgtca gctttggagg taattattgg caacagggag
     2041 cggcgctgat tgggtatgtt ttgtggcaga cgatacttgt atggggctat tttgtttatc
     2101 ggcaaaggaa aaatcagaca ggatggtttt ttgccgccgt tattttagcg ataattccac
     2161 tgtttttaac gaaagtgact cctttttttg aacatggaaa agcatcgtta cttggttttt
     2221 taggaatctc ttatttaacc tttaaatctg tacaaatgat catggaaata agagatggca
     2281 tgatcaaaac atataagcca tttcgttata tccagtttct gctgttcttt ccaacaatct
     2341 cttctggacc aatcgatcgc tataggagat ttgaaaaaga tcagctccat ccgcctgttc
     2401 gtgataaata tgttgatctt ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat
     2461 ataaattcat tatcggttat tattttggtc aagtactgct tcctgtagtg ggacgcattg
     2521 ccttacagca tggtggtatt tcatgggcat tagtaggtta tatgtacgtg tacagtatgt
     2581 atttgttttt tgattttgct ggctatagct tatttgcagt cggtacaagc tacatgatgg
     2641 gatatcagac accaatcaat ttcaataaac cttttttatc atggaatatc aaagaattct
     2701 ggaatcgttg gcatatgacg ctgtcctttt ggttccgtga ttatgtatat atgcgattga
     2761 tgttcttcat gatgaagaaa aaatttttta aaagcaagat cgtaatgtca aatatcggtt
     2821 attttgcttt gtttcttcta atgggtatat ggcatggatt gacgtggtac tacatcgtat
     2881 atggactgta tcatgcttgt ctgatctgtc tgacagatgc atggatccga tttaagaaga
     2941 agcataaaga gcagttgcct tctaacaaat ttacgcatgg cttagccgta tttttgacat
     3001 ttcaagcagt atgttttagc ttcctgattt tttcaggatt tttagataaa ctttttttta
     3061 gctaacgaat agtgacatat taatttatat aacaaaggag aaaaggaaaa tggaagaaca
     3121 aattttatca atcttagaag aaataacagg aacagatgaa gtaagaaaaa atcgtgatat
     3181 tgatctattt gatgaagggt tgcttgattc tttagcatct gttcaattat tagtcgagct
     3241 agacggagag ctggatatcc aagtccctgt atctgaattc gagcgagagg attggagcac
     3301 accgaataaa atcattcaac aagcaactgc attgaagggt tgagtatgaa aaagaaactt
     3361 tttgctattt tcggcccggt tgttttagct gttggcttac tgtttgtgtt ttttacctcc
     3421 tcctatcaag tagatatctc taatccagaa acgattagac gtgcctcttc ttccatgtca
     3481 gtgaacgtgt tgaaaggaga tgcgataaaa aattatgcat tatctgaaaa acaatatatt
     3541 cctttttttg gctcaagtga attaagcaga atcagtccat ttcatccgtc tgttctggca
     3601 gaaaaatacc agcgaaacta tcgtccattt ttgctgggag cacccgggac acaatcgtta
     3661 agccaatata tgatgatgcg ttctgcagga gatgctatga aaaacaagaa agtcgtcttt
     3721 attatttctc ctcagtggtt cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat
     3781 tattctgaac ttcaaacata cgactggttg ttttcaatga agaaagtaac gcctgctgat
     3841 cgctatttag ctagaagatt attaactttc tcaaaagtaa aagagaatga tacgttgacg
     3901 gcgattctgc aaacgatcaa aaaagggaag ctacctttac ctgaatcttt gaatcagctg
     3961 cgcagccaat ggaacatgct gaagagagag gatgaagtat tcagcaatat tgggttgaaa
     4021 gatcgacagc aaaaaatcga tcatgagtca aaacgattac caaaacaata ccaagaaaca
     4081 gaattatcca ttttagcaaa tcaaattgga gaacgagaaa cgacgaataa tccattcggg
     4141 ttgaaaaacg atttttatac gcatcgtatc cgagcccacg agccagagct caaacagtca
     4201 caaaaaaatt gggattatcg tttttcgcca gaattttctg attttcagct ggtcttggat
     4261 caattagcaa aaaaccataa cgaagtattg ttcatcatcc caccggttaa cgagaaatgg
     4321 agcgattata ccggactatc tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt
     4381 caattaaata gccaaggatt caatcgaata gctgattttg tcaatcaagc gggtaccaac
     4441 tattttatgg aagacacgat ccatctaggc tggaaaggct ggttggctgc agatcaacag
     4501 atcagaccgt ttttagaaga aaatcatatt actgctagta agtatcatct tgacgatgct
     4561 ttttttagca aatcttggca acatcaaata ccagataaac tacaattaaa ataaagaaaa
     4621 caggcgagtg ttgctcgctt gttttcttta tttttgaata ttctgaataa aagataaaaa
     4681 taccaagttt gcctgaacaa aaatcataag caatggtata atcacaatat gaatatagaa
     4741 atgggggaaa acatttggat acaaaagaag aacagctact tattgattta acttctgcaa
     4801 aaggagtacc tggaaatgaa gaagaggtaa gagaagtatt cagagaatat gccaaaccat
     4861 tcgctgatga tattttttat gacggtttag gcagtgtgat tgccaaacat ggtgctggcg
     4921 gaggccccaa ggtctttatt tctggacata tggacgaagt tggttttatg gtaacaaaaa
     4981 taaccgaaaa aggattcttg gaattccaaa ctcttggagg ttggtggggg caagtcatgc
     5041 ttgctcagca agttgaaatc aaaacacgtg aagggaaaat cgtccacggt gtgattggtt
     5101 ccaaaccacc acatgtttta acacctcaag taagaaataa accttatgaa ataaaggata
     5161 tgttcattga tattggcgca tcaagccaag aagaagccaa agagtggggc atacgtcctg
     5221 gagatatggg cactccatat attgaataca aacgaatgaa tggcagtaaa tatttactgg
     5281 caaaagcatg ggataatcgt atcgggacag ctgtttctct gcgagtatta gagaatctat
     5341 ctaaagaagc acacccaaat gttttattcg caggaagcga tgtgcaagaa gaagtaggct
     5401 tgcgaggtgc tcgaacaagt acacaccttg taaatcccga tattgctttt gctttagata
     5461 ctggtacagc aggagacact ccaggaatga caccaaaaga agcagattcg attttaggaa
     5521 aaggcccaca gattttgatt tttgatgcat ctatgatccc gcataaaaaa ttattgaatt
     5581 ttgttattga tgtagcagag gaaatggata ttccattcca atatacagtg atttctggcg
     5641 gaggaactga tgcaggacaa atgcatttga caagagatgg tgttccgtct gtagccatta
     5701 ccgttcctgt tcgttatctg cattcccata catcagtgat tcatgaagat gattatttga
     5761 atacagtgaa actagtcaca gaattagtga aacggcttga tagtgaaaca gttgaaaaaa
     5821 ttcggagtta tgaataatga ataatagaat acctatttct gaatatcgga aaaaagcacg
     5881 agtttctttg gaaaatcaat ggggaatcaa tacttggctt gttttcttat ctataatatt
     5941 tacgagcgtc atccagaact tttttgacgg tttcagtcct tttccggaag aaagcatgca
     6001 gcagcactta ttgagttttc tagtcgataa tttactttta ttcgcattca actatgcatt
     6061 gttttacatt gcgctagtag ttgtacgggg tggcagacca aaaagtaacc ttttattttc
     6121 agttttccaa agaaagtatt ttggttcgat acttggcatc aatttgatca attcagtcat
     6181 caactggcta ctcaacagtt taatctttct gcccatgttt ttaatcagcg gagtgagtac
     6241 ttatatgcag ctgcttttca gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt
     6301 tcttgatatc agctttgttt tatcgatgat agggatgatt tttgtttcag cactcatcat
     6361 gagtattgtt ggcggtttat tccaatttgc tgcatggaca aagttcgact atcctgattt
     6421 gtcggttatt caatgtttga aatatggctg gtttttattg aaagatagaa ttggtacgta
     6481 tattttgttg cagctgtctt ttatcggctg gtatttgcta ggagcaatcg ctttgttcat
     6541 cggtcttttg tgggtcattg tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc
     6601 acggattgaa aaaggaggtc cagacgccta ttttacttag catgtaagca gtttattcat
     6661 caaaaaaata acttttgtgg taggataaat ctttgagggg gaatgtacga tgaaattaac
     6721 tgttttagga tgcttaggag cgtatcctta taaaaatcaa gggacaacag gttatttgct
     6781 tcaatctgac gggttcaatt tgttgatcga tgcaggtagt gctacgctga tcaaattaca
     6841 agaacacctt gatccacttg atctagatgc tgtgatcctt agccattatc atcatgatca
     6901 tattgctgac cttggtgtat tacagtatta ctggcaactt catcctaaga gaaatccgga
     6961 gacattgctt tcaatttatg gacacacaaa agattccatt cactttgaag aattgacgtt
     7021 ggaaaatgtc tcaaaaggac atccttattt tgaagctgaa gaattaaaaa ttggtccttt
     7081 tctgattact tttatggaaa caatccatcc agtagtatgt tacgccatgc gtatcgtaga
     7141 agaaacaacg gggaaagtat tcgtatttac aggtgattca ggttatttgg aaagtttcgc
     7201 tgattttgcg aaagatgctg atttgtttct agcagatacc tatctttttg aaggaaatga
     7261 acatcatcat gcccatttca cttcaaaaga agctggagaa cttgcgaaat cagctaacgt
     7321 aaagaaactt gtacttacac acctacctca gtttggtgat ttagaacaat tgaaaaatga
     7381 agcgcaaaag gcagcaggca aagggattga tatagaactt gctgcagttg acaaagtgtt
     7441 taacatttag gaggaagaac atgatttttg taccaaatga aaaccacgac ccaagaatca
     7501 atttagcaat tgaacaatat ttgctgcaag aaatgccttt agatgaacct attttgctct
     7561 tttatatcaa tgaaccttca atcatcatcg gcagaaacca aaatacgctt gaagaaatca
     7621 atcgagatta tgtggaagaa aatggcattc atgttgtccg tcgcttaagt ggaggcggag
     7681 ctgtctacca tgattttgga aacctgaact ttagttttat catgcctgat gatggggatt
     7741 cattccgtga ctttgcgaaa gtaacacagc caatcatcca agcacttcat gaattgggtg
     7801 ttgctggggc ggaattgaaa ggccgaaatg atctagtcat tgatggcatg aaattttctg
     7861 gcaacgcgat gtatgctacc aacggtcgga tgtttgctca tggaacaatt atgttcgact
     7921 ctgatatcaa tgaagtagtc aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag
     7981 gaatcaaatc gatccgttct cgtgtaacga atatcaaacc atttcttcca gaagaaaagc
     8041 aaaaaatgac aacagaagaa tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt
     8101 cagtagaaga agtaaaaacg tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa
     8161 tctctgagga atattatcga aattgggatt ggaattacgg gaagtctcca gatttcaatt
     8221 tctctcgcca aaaacgtttt tctatcggtt cgattgaagt ccatttgaat gtctcagaag
     8281 gaatgatcaa agaagcaaaa atctttggcg atttcttcgg actaggagat atctctgacg
     8341 tggaaaaaaa attggttgga cagaaatatg ataaagcatc tctatcaaaa atagtcagcc
     8401 aaattgatgt gaagaaatat tttggtgcga tagaaccaga agaattgctt accttacttt
     8461 attgaaaata aaaaatagcg gaatccacat atatggattc cgctattttt tatacaggat
     8521 tcgggtaatt tcgagagagt tgaacagtga tccgatcaat gaaagtatct ttttcctgcg
     8581 ttagtgttcc tgtgtaacga tccgctacgt ctttcatcat cattaagtca aaacgttcct
     8641 gatcatctaa ttcttctgtg atgcctcgac taaataggtt tgtatcgtaa tgtgcactat
     8701 gttcgcaaca aatgacgaaa tgttcctctt gttcgtgtat cgacaaataa atttttcgat
     8761 acagtggatt ttcgatggca tgagtttctc taaaggaatg ctctaaagcg tggatcaaaa
     8821 gttgagccag atcgtcatcg ttgataggaa gttcttcggg aagatctgct tggaaggtta
     8881 tctgaatatt ccgtttggca gctaacttct ggtaacgcgc taagataaga tttgtcacct
     8941 ggtggttgca aaaagaacac agcatttcga aattttttgc ttcttctaat atcttttcca
     9001 catagttttt agcttttgtt tggtccgtat gcattaaatc ttgcagattc tccatctctt
     9061 ggacaaattc caggcgaagt gtatttactt cttggatatg ctgtcgaagc tgctcataat
     9121 gttgctccaa caaatcattt ttgattttta agaaatctac ttgtctccga tctttgtcgg
     9181 tatttaagat gtgatcgatt agattgtagc tgataataac gattagaatg ataatatata
     9241 aaatcgtaga ccaattgatc gttgtttgat agatacctaa tgccgtttgg atgtaaatca
     9301 agcaatggat aatggctacc aagacgatcc aaggtgtaca agctacataa aaacggttat
     9361 tcttatatgc ttcgccaatg catgcaagtg aagtgactaa agtgcttata acactagcaa
     9421 tattagcaat cggcattaat tcaggaagtt ctgcaggagt aaaagttgca taaagcagaa
     9481 cgccgataaa aatcaaaagt tggaacgtac aaaaaattcc gtaccatttt ttatagtaaa
     9541 acattcggct ccaatagtag cagatgatga aagctgacgt taagataata aataaatgag
     9601 ataaaaaaga atgaacgaat ggttcaaaaa gaataccact taaaatatct tctgatacag
     9661 cttcaaatcc taatgccaaa gcaaagcaac ttaagagaat aagtgagtat tcaagacgtt
     9721 tttgtttata catcaagtag atcgtataga aaaaggtccc tatagcgata aaaacagcga
     9781 ttatcatcgt caatacttgc ggcactgatt gtgaataaat aaaactaatc aatggtccag
     9841 ttgttcctag ataaactttc ggtggcagtc cagcatagtt ctgataagga gatgagactg
     9901 taagggtcaa tttttttccg atataatttg ctggtaaatc tatcacagcc aatgagagtc
     9961 cgggattgct ttcttcctga taatctttcc gttggtataa aatcttttcg cctagatata
    10021 cagtaatcca ttgatggttt cctttgatta gtaaatttgc ttcgtccaac ggtttttcaa
    10081 gtgtcgtttc cataatgaat gtttcgtctt tatccacggt aggaatcgta cgagtgtact
    10141 tgctgtcaaa cttaaaatca ggatcatctt ttgtatagaa aacccacgaa tcttttattt
    10201 ctttcatttg attgctgtcc gcaggagaaa aaataaaata aaagaaataa gcaattcctg
    10261 ttaaaaataa caatgataat ataa
//
LOCUS       DAHHPA010000090        10251 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.091, whole genome shotgun sequence.
ACCESSION   DAHHPA010000090 DAHHPA010000000
VERSION     DAHHPA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.091"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="NBH55_002264"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="NBH55_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCD1510210.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="NBH55_002265"
     CDS             complement(2203..4389)
                     /locus_tag="NBH55_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCD1510211.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="NBH55_002266"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="NBH55_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCD1510212.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="NBH55_002267"
     CDS             complement(4611..5051)
                     /locus_tag="NBH55_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCD1510213.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="NBH55_002268"
     CDS             complement(5125..5664)
                     /locus_tag="NBH55_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HCD1510214.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="NBH55_002269"
     CDS             complement(6279..7745)
                     /locus_tag="NBH55_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1510215.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8056..10137)
                     /gene="cadA"
                     /locus_tag="NBH55_002270"
     CDS             complement(8056..10137)
                     /gene="cadA"
                     /locus_tag="NBH55_002270"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCD1510216.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         2826 a         2259 c         1735 g         3431 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tgttatttta tgtgtgtatt cttttattta atacacatcg
     7861 ttttttcata ttttttccat caaaaaaata agttattttc ttttatttca tttaccttat
     7921 tgaatttcaa gctattttga attttctgca aaattaaaaa cattagttat tttataatcc
     7981 tatctatttt ctttacattt ttcattaaga aaaggatata aaaaaaagag gacgttcatt
     8041 cgtcctcttc attgatcaac gtttattttt tacatgcaag cttcgcatgg cattcaaaac
     8101 agctaatact gtcaccccaa catctgcaaa tactgcatct cccattgatg caaaaccaaa
     8161 agcacctaag gcaagtacca aaatttttat cccaatagca aagacaatgt tctgtttgac
     8221 gattttcaat gttttctttg ctaaatggat tgcttctgca atcttcgatg gttgatcatt
     8281 catgatgact acatctgctg cttcaattgc agcatcgctt cccaatccac ccattgcgat
     8341 tcctacatct gctcttgcta gcaccggtgc atcgttgatt ccatctccga caaatgcggt
     8401 ctttttattg tttctttgta agatttcctc taaccgctgc actttatctt ctggaagcaa
     8461 ttcgctatat atcttgtcca cgcctacttg cttcccgata tgatcagcaa tttttttcga
     8521 atcgcccgtc aacatgactg tgttcttcac accagccttt tttaattgaa ccaatgcgtc
     8581 tacggcatct tctttcaagg tatctgcaat aacaagataa ccactgtaac tttgatcgat
     8641 cgctagataa agcaatgtac cgatttcttg acttgaagga aaagagattt caaatctttc
     8701 catcaatttg tgattacctg ctaatacttg tttgccatca acttcagcta agatcccttc
     8761 acctgcaaat tcttggatat tagctgttgg taaaagcgga cctgcgtagc cttctaaaac
     8821 agattgagca atcggatgtg tagaaaactg ttctgcactc gcaacatatt gcatgaatat
     8881 ctttggatcg atttttgtat cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc
     8941 ggttttatcg aataccactg tctcagtttg tgcaagcaac tccaagtaat ttgaaccttt
     9001 tactaataca ccgattttac ttgctcctcc gataccgcca aagaaactaa ggggtacgga
     9061 gatcactaat gcacatggac atgagatcac taaaaatgtc aatgctcgat agatccattc
     9121 agaaaacgcc gctccagaaa caacaagtgg aggtaaaaca gctaataaaa cggccaatcc
     9181 tacaacgact ggtgtgtaat agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt
     9241 tttactgctg gcattttcta ctaattctaa taatttgctg atagtagaat tttcataaga
     9301 tttttctact tgaactaaaa atggtttatt tttattgata aatccactta atactgtttc
     9361 accaacatat acagatttag ggactgattc tcctgtaagt gctgacgtat cgactaatga
     9421 ttctccttcg atgatcgttc catctaacgg tacacgttca ccaggcttca caagtacaaa
     9481 ctgaccgatc tgcacgtctt cagggttcac ggtcatcaat ccttccgtcg tctgcacgtg
     9541 agctgtttct ggacggatcg ccatcaattc gcgaatcgat tttcttgaat gactgactgc
     9601 caatccttgg aaatactcgc cgatttgata aaatagcatc actgctacgg cttctggata
     9661 ttcaccgatc aacatagcgc caatcgtagc aacagacata agaaaattct catcaaagat
     9721 ttttcctttg acgatattca tcactgcctt cttcactaca tcatatccta tcagcagata
     9781 aatcaataaa taccctatca agcgaattgg ttcattaaaa tcaaataata gcaataaagc
     9841 gatacctgca agggaaaata aaatccggaa cagcgtattt ctctcttttt catttccatg
     9901 ttcagatact ttttccttat ctatttctgt tactttgaca tctggttcta attccttcac
     9961 tacttttttt atttcttgtt caatcgtagc caaatgatct tcctctgcgt caatcgttag
    10021 ttttccagaa gtgaagttta ccgttgcttc cttgactcca ttgatcttct gaactccttt
    10081 ttcgattttc attgcacagt tcgcacaatc taaaccttct aatctgtaac ttttcatttt
    10141 aatgacctcc tcgcctcaac atatgaatat ctcttcataa ctaattataa atgaacggtt
    10201 attcatatgt caaggtatcc gaaagaaaaa atgaaaaaca tctgcatcgg a
//
LOCUS       DAHHPA010000091        10233 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.092, whole genome shotgun sequence.
ACCESSION   DAHHPA010000091 DAHHPA010000000
VERSION     DAHHPA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.092"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..769)
                     /locus_tag="NBH55_002271"
     CDS             complement(<1..769)
                     /locus_tag="NBH55_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HCD1510217.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQSFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNAL"
     gene            complement(974..1324)
                     /locus_tag="NBH55_002272"
     CDS             complement(974..1324)
                     /locus_tag="NBH55_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510218.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1919..2365
                     /locus_tag="NBH55_002273"
     CDS             1919..2365
                     /locus_tag="NBH55_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCD1510219.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            2989..3981
                     /locus_tag="NBH55_002274"
     CDS             2989..3981
                     /locus_tag="NBH55_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1510220.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            3997..4881
                     /locus_tag="NBH55_002275"
     CDS             3997..4881
                     /locus_tag="NBH55_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD1510221.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            4930..6537
                     /locus_tag="NBH55_002276"
     CDS             4930..6537
                     /locus_tag="NBH55_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1510222.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            6538..8022
                     /locus_tag="NBH55_002277"
     CDS             6538..8022
                     /locus_tag="NBH55_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCD1510223.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            8199..9191
                     /locus_tag="NBH55_002278"
     CDS             8199..9191
                     /locus_tag="NBH55_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1510224.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETIEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            9243..10112
                     /locus_tag="NBH55_002279"
     CDS             9243..10112
                     /locus_tag="NBH55_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD1510225.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         3229 a         1625 c         2193 g         3186 t
ORIGIN      
        1 ctaaagcatt gtaaaaaaat ggacgtctaa ctatatattc gcttgtatat agtatatctt
       61 gattcgtgga atcatagggt ttctttacca tctttttggt gacagttggt agtttgagat
      121 tccgggactt gaaaaaagct tttttcttat gctctttcaa ttccagctct ttatcgaaat
      181 atttgctgat atagcgcccg cgattttcga tactgtctac atcgatcttg ttgatcttaa
      241 cgaaactctg accccaaatc gtagttaacc ggttaaatga gataaagggg aaatcaaaca
      301 agataatatg gtagtggatt gcaccgcgtt gctgcttttc ccacgtggcg agatacttta
      361 aatttgcttt tttagtttta tagaggtcat aattcagtcg tttgataaat tttttaaatt
      421 cattatttgc atactcaatg tcttgaatgt tttctttgaa agtgagagtt aaaaatttcg
      481 tttgattgtc aaaattcgtg tctacaaggc gggcaatctc aaatcgcata ctttgataat
      541 gtttctgttt gcgttttaaa ctgtcatatt gtccttgagc ggataattca tcatagtttt
      601 ttcttttttc aagagtaata tcattttcat aaatccaagc taatttttgt tttcgttcca
      661 gatttacttc gttttcgatc gttttacgtt tgggagtaat gattgcttca taatcataaa
      721 tttcaatata cgtgggagtt tcaatgattt tctgattata agcttgcaaa aatcatcact
      781 ccttgttgcg tattcgattg tcaaagaacc agtcattttc tcctgatgtt gatctataaa
      841 tcaagttaag ggggaaggcc tgcgtagcag cccttctagc ctatggggtg ttgggcggtc
      901 ttaacggaca gaaacggctc tgtgggcgct gccccgccgt ttcgtcctaa ccacccaaca
      961 ctccatagta tagttagttt tgtgtagtgg taccttgttt tctgggaatc aaagttgccg
     1021 tatcagcggt tgccgataac ccataactga caaattgtcc attcgagcgt ccccaaggta
     1081 caatcaccaa attggtaaat gtaattatgg ctaaaccttg ttgggtaagt tcttccggcg
     1141 tgatcacagg atgttcagat ttgactttga cactgatttt ttcgtagcga agttttggta
     1201 ggatacattc gtagctccaa cctagaattt cgccctcctt ttgccagcga gtgacattga
     1261 cgacttggta ttctttttcg agtgtttctt ttggtaaact gagatcggta atttttattg
     1321 gcatcacaat tctccttttt tatacgaaag tttatacgat ttgattaaaa aatatagatg
     1381 gccatctacg acttgtatta tatacgagaa cttatacgta gtcaagagat tttctaaaaa
     1441 aatatatacg attttttata cgagtgttat aatgaaaaaa atgataagga ggaatttgaa
     1501 tatgtttggt ttagtacaac ctaataaagt gaaggtgggc caacgaatca aagaaataaa
     1561 agacggaatg tctctctctt ttacagaatt agggaatcga ttaggtttaa aaaagccgac
     1621 gatcagttcc tacgttcaag gctatgcgtt ggctcctaag agtgtgatca accaattatc
     1681 tagtatcagc ggaaaacctg tcggctggtt ttattttggg catgtggaag aatatatccg
     1741 agagtatttg tttctcattg gtcagaaaaa aatagtggac gattatcctg aaatcattga
     1801 gaaaataaag caagaatttt atacgggcga gttcaaaaat cctgcttggg agaatgagct
     1861 aggctatcct ttggaggaat ttatccaaga atgttttgta gaatattcga gtgaaatcat
     1921 gaaggactat gtcgtacaga ttacgaaaga ggtacttgaa gaatccaaaa cgctaaacaa
     1981 actatcatca aaagcaaaag aagaagctgt cacgattctt tcatctggga tcattgacta
     2041 tatcgagatg agtggtgaga tcaagtatgg agagaaagat cgcattgcga aaatggtgaa
     2101 agaatcaata aaaacctttg atgtgaaaca agacctgaca tttaatgatt cctatttgat
     2161 tggaaagttg atcaatattc ttggtacaga tgaagatacg gagctgtttc tttcttcttt
     2221 atcaatgata tttactgata agcatttttc cactcgattt ggtggggaag aattagtcga
     2281 aatatttcaa tcgatgcgac cagcgttaat caaactctat cgggagaaaa caccagatga
     2341 attttatgat tggtttgaga agtaaaataa gaaaagtggc ttagattaca agttggtgaa
     2401 aaaatttttt tcagcataac atcagtaatc tataaagggg ttgttgatta tactgtagaa
     2461 gttaagtact caagagattt tttgtacgta aacaaagcaa aaaagggtgc agtttcacta
     2521 gaaaatgtac tcttttttgt gatttttctt agttatagag tttttttgaa actttaaaaa
     2581 tgagaaaaca tattgatttt aaagagtgta gacatagctt cttttgctaa atagattaaa
     2641 cttactggtg agtggtcttg aacatacttt taaatcacat tttactgagt tttattggat
     2701 tttataattc attggaaacc tcgtacttaa tctgttttta tctatcgact agaatctgta
     2761 tatattccag ctatcaatga ggccgcagaa aagggtgctt aaaaataatc tgttgtttaa
     2821 aatattatta tcagaatact tagatacaaa attctggcac aaagtaaaaa aaatattgac
     2881 ttaaaaaaat ggatatggta atctataaac gaaaatagtg gtaacgtttc caaaatgcat
     2941 cttcgttaaa tttttatttt tttaaacgaa aatggaaacg tttctaaaat gtattgggag
     3001 gagattgaat tggaaaagac attgggacca ggtggtgaga taagggcggt acaacgggat
     3061 aaaaaaaata tttcgaagtg ggaaaaatta aagaaacagt tgcggcgtga taagtggatg
     3121 tacttgttgc tcttaccagg tatcatcatg acgtttatct ttaagtatgt gccaatgtac
     3181 ggtgctgtga ttgcatttaa agactataat cctatgctag gaattttgga aagtccttgg
     3241 gtaggactag atcactttag ggaatttatc agttcaccaa attttacgct tttgctgagc
     3301 aacacattaa aaattagtat ttatggttta ttgttaggtt tttttccacc aattatttta
     3361 gcactgagct tgaatcaaat tatcaataaa aaaataaaac agaaaatcca gttgattcta
     3421 tatgcgccga attttatctc agtagttatt attgtgggga tgctgttttt gttcctatcc
     3481 gcaaatggac ctattaatgg gttagtagaa aaggttacgg ggcaaccaat tatgttcatg
     3541 tctaatccag cttatttcag aacgatttat attttatcgg gaatttggca aggaatcggc
     3601 tggtcatcca ttctttacac cgcaacgctg gcgggagtca gtaccgattt atatgaggca
     3661 gcaaatatcg atggggcaaa tattttacaa aagatttggc atatcgattt gcctgcattg
     3721 aggcctgtga tggtgatcag cttcatcttg gccgccggcg gtattatgaa tgtaggatat
     3781 gaaaaagcct acttgatgca aacttcaatg aacatccctg cctcagaaat catttccact
     3841 tatgtataca aagtcggctt gcaatcaggt gattatgcct actctgctgc tgttggtctg
     3901 ttcaatatga ttattaatgt cgttttatta ctgattgtca acaatataac caagcggcta
     3961 aacgatggtc aaggcttgta atgaaaggag aaaattatga atagtgcttt atcaaaaatc
     4021 gaccgtcaaa tcttatggtt gaatcgagtg attgtaactt ttttagtcgt catcaccatt
     4081 gttccgctga tttatgtact ggtagcttct tttatggacc cagctacttt gatcaataaa
     4141 ggtataagct tcaatccgaa agattggaca atacagggat atcaacgggt atttgcagat
     4201 gattccattt tacgcggttt tgtaaattcg ttgttttact cttttagctt cagcttattg
     4261 actgtattta ttacgatgat cactgcgtat cctttgtctc gacctaactt ggcggggaaa
     4321 aagattatta tgactttttt tgtcattaca atgttcgtgg gtggtggctt gatacctacc
     4381 tatttattgg taaagaactt gggcatgtta gatagtgtat gggcgattat cgtaccaggt
     4441 gctataaatg tgtggaatat tatcttagcc cgtacctatt ttagtgcttt gccaaaagaa
     4501 ctttctgaag ctgctactat cgatggcgcc aatgacttgc aaatactgtt caaagtcatg
     4561 cttccattag cgaaaccaat tatgttcgta ttgtttttat atgcctttgt tgggcaatgg
     4621 aactcatact ttgatgccat gatttacatc aaagatcccg acttacagcc gttgcaactc
     4681 gtattaagaa atattttgat ccaaaaccaa ccttcacaag acatggtggg agccaatact
     4741 gccatggcag aaatgaaaca aattgctgag ttgatcaaat acgcaacgat cgtcatttct
     4801 agtctgccat taatcataat gtatcctttc ttccaaaaat attttgataa aggtgtttta
     4861 gttggttctg ttaaagggta agtattgttc actatttgta tgaataaaaa ttataaagga
     4921 gattttaaga tgaaagcaaa atggatgctg ccaatcgttt ttacttccgt actaattttg
     4981 ggggcttgtg gaggctcaaa agaaaagagt aaatcaagcc ctgattatga actagaaaat
     5041 gtctcttttc cattgaaaga aaaagtatct ttaaaaatga tgtctcaaag tgcccctttg
     5101 gctcccaatg atcctaatga caaattgatt ttccaacgat tagaagaaga gaccaatctc
     5161 catattgatt ggacaaacta cagtagtgat tttgccgaaa aaagaaattt ggatatcgct
     5221 agtggtgatt taccagatgc catcttcaac gccgctgcag gtgactatga tttattaaac
     5281 tgggcggaaa gtggcgttat tgtaccgatt gaagatttga tcgaagatta tatgccaaat
     5341 ctgaagaaaa tttttgatga aaaccccgaa taccgacaaa tgtctactgc acctgatggt
     5401 cacatttact ctatgccttg gattgaagaa ttaggcgaag gaaaagagtc gattcataca
     5461 gtaaatggga tggcttggat caacaaggag tggttggata atttgggtct tgaaatgccg
     5521 caaaccactg atcagttgat gactgtgttg gaggctttca agacgcaaga tccaaatggt
     5581 aatggtgaag cagatgaaat tccttttagc tttattaaca atggaggaaa cgaagactta
     5641 aaatttttaa tgggggcatt cggtctaggt gataatgatg accacttagt cgttgataac
     5701 gatggtcagg tacaatttac tgctaacaaa gaagggtata aagaagccat caaatatttc
     5761 aatgaaatga ataataaagg tcttattgat aaagaggctt tcgaacaaga ttggaatacg
     5821 tatgttgcca aagggaaaga acataagtat ggcgtgtact ttacttggga taaagcaaat
     5881 atcacgggtg cagatgatag ttatgacgtc ttgccagtct tagcgggacc agatggaacg
     5941 aaacatgtta ctcgtacgaa tggcatgggt ttctcaagag atcgtttcgt aattactagt
     6001 gcgaataaaa acttggaact tacagctagg tggatagata aaatgtatga accgttacag
     6061 tctgtccaaa ataactgggg aacctacggt gatgaagcac aggcgaatat ttttgaatta
     6121 aaggatggta tgttaaaaca tttgccttta gaggggacag caccggggga actaagacaa
     6181 aaaaccgaag ctggaggacc attagcagtt cttgattctt attacggcac agtcactact
     6241 atgccagatg atgcgaagtg gcgtttagat ttgatgcatg acacgtattt gccatatatt
     6301 acagaagaca atatctatcc acgtgtcttt ttagaaaaag aagaaacaga tcgattagca
     6361 aaaatcgaaa cggatatgtg ggattacatc tatcgcaaaa gagcagagtg gatcaccggc
     6421 ggtaaaatcg atgaagaatg ggaagaatac cttgctgaat tggaacgtgt aggtttggaa
     6481 gaatggattc aaataaaaca aactggctat gataattttt tgaagggagc acaataagtg
     6541 ttagtagaag aaacggcgat tttggataaa gcaaatcgat ttattgaaat gatgcgagga
     6601 aaagttaacc ctcagtatcg acaaaactat catttttccg cacccatcgg gtggataaat
     6661 gaccccaatg ggtttatata ttacaaaggg cattaccatc tcttctatca atactatcct
     6721 tacgatagtc gatgggggcc catgcattgg gggcatgcgc gcagtagtga tttggtccat
     6781 tgggaagatc taccagtagc gttagcccca gatcaacctt atgacagcgg cgggtgtttc
     6841 tcaggaagtg caattgaaag agacggaaaa ctatatttat tttatacagg ctgtcaagaa
     6901 attgatggta ttagccatca gcatcaatgt ttggcggtct ctgaagatgg tattcatttt
     6961 agcaaataca gtggtaatcc tgtggtagca gaaaaacagc taggtgaaaa tgggctaata
     7021 caagaatttc gtgatcctaa agtaattgtt cacaacgatg gttactatat ggtagtagct
     7081 actaaatcta cacagtctac tggtagaata gtgatgtttc aatcgtcaga tttgtatact
     7141 tggcactttt actctgtgct gctagaaggg acttcagagc aaggaataat gtgggaatgt
     7201 cctgatttat tccatttaga cggacgagat gtcttaatca tgtctccgat acaaatcaag
     7261 aagaacggac tggaatatca taatatcagt tctaccatgg cttgtattgg tgaggtaaac
     7321 tgggaaacag gtcagatgat agttgataat ttccatgaaa tggattttgg aatggatttt
     7381 tatgcaccac aaacaacaag agatcctaat ggtggacggg tgatgattgc ttggatgcag
     7441 atgtgggatc gtacattgcc tacacatgag ttaggccatc tttgggcagg ctctatgagt
     7501 ttgccccgag agttaagagt gaaaaaaaat cgattggtac aaaaacccat ctcaactgta
     7561 tatagtcatt tagtttatca acaaggcagt gaaaatattg cagtgactga cacatcagtc
     7621 gtatttcgaa acgcgctgtc tgattcttca tatttacatc tagtcgcaga tttggagaaa
     7681 gctgaagttt ttacaattga attactgcgg tctaagacgg attgtctcaa actagcctat
     7741 gacactgcta cacaggtttt tgaattggat cgtcagaaac taggaagaga gcttacaggg
     7801 gcggaagaag accctctgaa tgtacgaaaa gttgaagtac ctttgattga taatcagctc
     7861 gttcttgaga tttttagaga tacctcatca attgaagtct ttattaatgg gttagaagtt
     7921 atgacggcta ctttctatga gctggaaaaa gctcgagata tcttgtttca ttcgatcgga
     7981 gaaagtcgta ttcaagcttt tgaaacagcg agtgtgaaat aaatgtttga ttgcttaggc
     8041 gatgaatcaa ttatttaata ggttttgaag gataatgttt ttagaaaaga acagatagct
     8101 agatttccca tggatttgct attagtattt tgaaaaaatt agtaatttat gacaaaattc
     8161 attatactaa acttgtgcag aaatgtgagg gatcaactat gaaagcaaca atgaaagatg
     8221 tcgcaaaatt agcaggtgtt ggagtaggaa ctgtttcccg ggtattgaat aacggttctg
     8281 ttaaagcttc tactagagaa aaagttgaag cagcaatgac agagttaaac tatcaaccag
     8341 atttttatgc ccgggggtta aagatgaagc atacgaacac ggttgctttg attctaccca
     8401 cggtatggca tccatttttt ggcgaatttg cctaccatgt agaaacagca ttagaaagac
     8461 aaaactacaa gctttatttg tgtaactcca gtggtaatag tgaaaaagaa caagaatata
     8521 tccagatggt cagccaaaat aaagtagacg gcatcattgg aattacttat tcagatatcg
     8581 atgaatacat ttcgagtaat ctgccttttg ttagtattga ccgttatttc actgaagagg
     8641 tgatttatgt gaccgctgac aatgcggccg gtggacgttt ggctgcaaat gaactttatc
     8701 gccgaggatg tcagaatgta gcatatatcg gtgggtacca agaaacaccg aatgaaacga
     8761 agagtcgtcg aaaatatttt gaaatcgagt gccgtcaact ggggatttcc tataaagcgt
     8821 tagatatgtt agaaccttta gaccatctct cagaacgtgt aacaaatttt ttgttagaga
     8881 atcccgatat cgatgggatt tttacgatca acgatccaat ggcattagct gtcattgata
     8941 tgcttgaaaa aatagggaaa aacgtaccag aggatgtcca agttatcgga tttgatggtc
     9001 aaaagatgac agaaggagat ccttatctag tctcaacgat tgcccaacct acaaagcaaa
     9061 tggcggaagt ggcagtggca aaattaattg ctgtcattaa tggagagaca atagaaaaac
     9121 gtaccgtatt gccgattgaa tttggcgaag gtgacacaac gaaaccttta gtgagaaacg
     9181 attttgctta gtgcaaatac gtttcttttt ttgaaaatga gtgaatgaaa ggtgtagata
     9241 aaatgttata tggtggaata gaagccggtg gaacaaagtt tgtctgtggg attggtaatg
     9301 aacaattaca agtgatagaa cggaaaagtt ttcctaccac aacaccagaa gaaacgatgg
     9361 agcaagtgtt tgatttcttt gataagtaca aaggaaatat cgcatcaatt ggtgtaggag
     9421 catttgggcc tattgatatt caaatcgaat caagaactta cggatatatc accaacacac
     9481 cgaagttagc ttggcaaaat tatcattttg taggagcttt ggaaaacgcc ttgtcggttc
     9541 ccatctattg gactaccgat gtaaatgctg cttgttatgg agaatatatg gcagggtatg
     9601 gaaaaggtaa aaatagtgtt ctgtattaca ccgttggtac aggtgttggt ggtggtgctg
     9661 tagtggatgg gaaaatagtc gcaggtttta gtcatcctga aatgggacat atgctcttac
     9721 aacgtcataa tgaagatcaa tttgaaggaa tctgtccttt tcatcatgat tgtttggaag
     9781 ggttagcatc aggacctgcg attgaaaaac gctttggaag aaaagcccag actatctcat
     9841 ttgatcatcc ttattggacg attgaggcag attatcttgc tcagtgtgtg tataacacaa
     9901 cgttactata tgcaccggaa gtaataattt taggcggggg tgtaatgaaa cagccgcaac
     9961 tactcgagtt gattcgccaa tcgtttaaag aaaaaatggg ttcctacgtg gatacgccta
    10021 ctctcgaaga atatctacta actccaaggc ttgctgacaa tgcgggcttg attggctgcc
    10081 tagctttagc aataaagaaa ctcaatcatt agaatagtat ttattagaac tattcataac
    10141 ataccggaag tttaaaaggg tattctgaaa cttaaatagt ctcaattcat tcttctttct
    10201 tgagaatctg agtagagact tttattctgt ttc
//
LOCUS       DAHHPA010000092        10232 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.093, whole genome shotgun sequence.
ACCESSION   DAHHPA010000092 DAHHPA010000000
VERSION     DAHHPA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.093"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            219..2384
                     /gene="acm"
                     /locus_tag="NBH55_002280"
     CDS             219..2384
                     /gene="acm"
                     /locus_tag="NBH55_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCD1510226.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPGATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2656..3141)
                     /locus_tag="NBH55_002281"
     CDS             complement(2656..3141)
                     /locus_tag="NBH55_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCD1510227.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3231..3576
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3621..4262
                     /locus_tag="NBH55_002282"
     CDS             3621..4262
                     /locus_tag="NBH55_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD1510228.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4281..4916
                     /locus_tag="NBH55_002283"
     CDS             4281..4916
                     /locus_tag="NBH55_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD1510229.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4934..5749
                     /locus_tag="NBH55_002284"
     CDS             4934..5749
                     /locus_tag="NBH55_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1510230.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6068..7504
                     /locus_tag="NBH55_002285"
     CDS             6068..7504
                     /locus_tag="NBH55_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCD1510231.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7693..10140
                     /locus_tag="NBH55_002286"
     CDS             7693..10140
                     /locus_tag="NBH55_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD1510232.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         3574 a         1672 c         2203 g         2783 t
ORIGIN      
        1 gtaaaataaa atgaatatat gcgaaaaaat aatttacatt aggaaaaacc ttgtaaaaac
       61 agttttttgg catatttatt ttttataaat atttgtaaaa taactatcaa ttttttagtt
      121 aaaagattgg aaatgagtgg gggaggaatt gaagaaagga tttttgagag atgatatagt
      181 aggttttatt tttagttaaa ataggggaga tagaaaaaat gaaaaagtgt agaaatatta
      241 ttttatcaat gttatttatt attactaata taacatcact gattcctgtt catgtctatg
      301 cggatgcagg cagagatatc agcagtaatg tcacttcgtt gaccgtggac ccaacgaata
      361 ttacagatgg aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc
      421 aaccaggtga ttatctttgg atcaattggc caagtgaagg aaatattcgc ggtgaaggat
      481 tccaaaaaga aatcccatta atgattgaaa ataaaaatgt tggaacttta acggtcagaa
      541 aagattctgc gcaggttgtg tttaatgaaa atattaaaaa cctagactca gtagaaggtt
      601 gggggcaatt tgagatacag gccagaaacg taaccgatac cggtgaagaa aatacgggac
      661 cattcaccgt gacgagcggt gataaaacag ctactgtaaa tgtgacaaag ccagcttctg
      721 gttcttcttc tagcgttttt tattataaaa caggagacat gctacctgaa gatactaagc
      781 acattcgatg gtttttgaat attaacaata acggcactta tgttgaacaa ccagttaaaa
      841 tcagtgatga aatccaaagt ggacaaagat tagatccaag tacgtttgaa attaatcaaa
      901 tacacttagg tgaacagaag gtttatagag gagaagaggg aatccaacaa tttttacaag
      961 atttccccgg cgctacattt aatttttcag taactgataa ttacattgaa ataactatac
     1021 caaaaaactt tgtcaatctt aggaaaataa tggtgagtta taagacgatc atagaaaacc
     1081 cagaacaaat aaattttgag aatcattcgg aagcttggtt taaagaattt aataaacctg
     1141 ctgttgatgg agaaagtttc aatcatacag ttaaaaatat ttcagcttct ggtggagtca
     1201 acggtacagt tagaggcgaa ttgaagattt tcaaatatat caatgacaca gagattggta
     1261 tcccaaatgt gactttcgaa ttaagacgag cagatgaaca gcctattcaa ggacagtcta
     1321 gtatcctgtt gacttcgaat gaacagggag aagctagtat caaaggcctg caagtaggag
     1381 actatgttgt aaaagaaaaa gaagcaccca attggattga ttttgatcca ctaagttcaa
     1441 atgaactcaa attctccata aacgaaaatg atacagaggg agtcagttta cctatttata
     1501 acaagaaaaa ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa
     1561 gaccgagtat ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg
     1621 atgcagaaac aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac
     1681 agtatgatga tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa
     1741 atgattatgt cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga
     1801 ataaagaagt catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg
     1861 gcaaacgacc gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca
     1921 aagaagtaag tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag
     1981 acgggcaaga aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga
     2041 ttgacgggta taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag
     2101 gtagccatat aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa
     2161 atctattctc tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa
     2221 gcaattatat tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc
     2281 ttcctaaaac aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttgatagtaa
     2341 ctatctgtgc tgggatattc ttacaaaaat ataaaaaagt ttagttagaa tataatattt
     2401 tttgttaaat atgactgaaa tcaaaagaat aaaattccga tttataatcg aaaaaagaag
     2461 catttttata tatgcttctt ttttcgtaga atcaataaat atggatttgg ctaactatgg
     2521 aagaatactc gtattagttt tcagcgttca tactagttgt tacattgttt gattggccaa
     2581 aagaatttct gatatatttt gtgctaaata aagaaaaaga cgcttgaatt gggaaataag
     2641 aaaactactc tatcattaca aattttcata aactggcaga tgttctaagt aaacaagtat
     2701 cttttcaaag tcggaaaaat ccataatcaa cgtagtggta ttgatatttg gatgaaagcc
     2761 tacggtatct tgacgatcaa tagcagcatc gatcacgact tggatttttt tctctttgtc
     2821 gtgcatcaat gctaatggag tcactgtacc tgctggaacg cctaggaggt caggaagctg
     2881 ttcttcagaa acaaaagata agcgcttttc ttttaattgt tcagctagtt gttttaaatc
     2941 ggcgtgcttt tcatcaggca agatgacaag atagatttgt tttccttttc ttgatttcaa
     3001 taatagattt tttacttgcg gaccaggtag ggaaaaaggg acatttttga ctgacgtaat
     3061 cgcaggatga tcgacttttt gaaaagagat acccagtttt tttagtagtt cataagcttc
     3121 ttgttcggtt gcacgactca tcgcgcatca ccctcctttt tttattagtg tatcataaaa
     3181 aagagttgac aaatgaaaaa taatcatgta aattacacgt aaagttaaaa caaatgacat
     3241 cgaaaagaag agtagccgtt aggtgttttt agagagtctc tggttggtgg aaagaggcag
     3301 cagttaaccg tgaaacacat cttggagtcg attttctgaa gcgtatagta ggaaaatccg
     3361 gggagcccgt tacagctgcg gtcgttgcta gatgaccaga taaggctggc ttttccgtga
     3421 ggagtcggta aaaaaaggtg gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg
     3481 actttttgtt ttcatctgaa cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat
     3541 aaacagaggt ggaaccacgt ataatacgtc ctcttggcat ttgccaagag gattttttta
     3601 ttttaaagga ggaaaagaag atggagtatt tgattgaagt tatgccgcaa ttattagtag
     3661 gagcactcac aacattgaaa ttatttttat ttaccttgat tggctcatta ccattaggga
     3721 ttgttttagc attaggattg tctttgcatt ttaagccgct acgctacttg cttcaaacat
     3781 atgtatgggt tatgcgggga acaccgctgt tgttgcaact gatttttatt ttttatgggt
     3841 tgccgacgat tgggatcgtt tttgatcgtt tcgaagcagc actcattgcc tttatcttaa
     3901 actatgcagc ttattttgca gagatctttc gtggagggat acaatcgatt cctcaaggtc
     3961 aatatgaagc agcgcaagtt ttgcaattaa gtccctttca aacggtgacg aaaatcgtca
     4021 tcccacaagt gacaaagatt gttttacctt ctgtagggaa tgaagttatc aacttggtca
     4081 aagattcttc cttgatctat atattaggtt taggtgacgt aatgcgtgct gggaaaatcg
     4141 cgatggaacg cgacgcgact ttactgcctt tgctaggcgt ggcaatcatc tatcttacat
     4201 tgactggtat tctaacaatc gtaatgaaac aagttgaagg aaaattaaat tattaccgct
     4261 aactgatagg aggaagaaat atgattgaat tgaagaaagt gaacaaagca tttggtgaaa
     4321 aaaaaataat caaagatttg aatctaatca ttccagatgg gaaaacatta gctatcgtag
     4381 gtccttccgg tggcggtaaa accacgcttt tacgtatctt ggctggctta gaaagttctg
     4441 acagcggaga attttattta gacggtgtac cgttcaaccc attcgagctt aaaatgaaag
     4501 aacaagttgt aggagtcgta ttccaagact tccagctatt tccacatctg aatatttttg
     4561 aaaacattac cttagcacca aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa
     4621 aagcagaaga attagcggct tcattaggga ttagtgattt attgaatatc tatccttatc
     4681 agctttctgg cggacaaaaa cagcgggtag cgattgccag agcattggct atgaatccac
     4741 gggtattggc ttatgatgaa ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg
     4801 agcgtttgat cctatcattg aaagaagatg gggtaaccca aattattgtc acccatgatt
     4861 tagtatttgc agaaaatgtc ggtgatcaat tactaaaagt ttccccaatt cattaagaaa
     4921 ggagaagata gagatgaaaa aaaccagttt tctattcgct ttgatcgcag gtcttcttct
     4981 ttttgcaggc tgttcgaaca aaaaaacttc agcagataat tgggcgacta tcaacgaaga
     5041 gaaaaaaatc gttataggtt tagacgatac atttgttcct atgggattta gagataaaga
     5101 cgggaagctc acaggatttg atattgattt agccaaagca gtctttgaac aatatgggat
     5161 tacggtagac tttcagccca ttgactggtc aatgaaggaa tttgaactga acaatggaac
     5221 gatcgatttg atatggaacg gctattccaa aacgccggca agagaaaaga aagtgcaatt
     5281 tacaaaacct tatatggaaa acgatcaagt gctgatcaca cctaaagaat caggaatcac
     5341 aaactttgga caaatgaata ataagagatt aggtgcgcaa aatgggtcat ctggttatga
     5401 tgtatttacg aaacaaccag aagtattaaa agagatagtc aaaaaccaag atgctgtttt
     5461 gtatgatagt tttaatgaag cattgatcga tctgaaaagc agacgggtcg atggtctgct
     5521 gatggataaa gtatacgcag actattattt gaaacaaaga aacgaactag aagacttcaa
     5581 cataacaaaa ggttcttatg agagtgagga ttttgcagtt ggtgcacgca agtcagatag
     5641 tacattagtg aaaaaaatca atgaagccct agatcaacta gagaaaacag gtgaattcca
     5701 aaaaatatcc gataaatggt ttggcgaaga tgttacgccg agaaattaac aaatagggga
     5761 ctgtgacaaa agacgtgtca cagtcccttt ttccgaataa acagtgttct cctgccaact
     5821 ccttgtgact cggagctgaa tggcaggctc acaacctctt ggtaaacggt gttcaaaagc
     5881 cagtttttcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt
     5941 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
     6001 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
     6061 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
     6121 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
     6181 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
     6241 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
     6301 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
     6361 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
     6421 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
     6481 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
     6541 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
     6601 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
     6661 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
     6721 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
     6781 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
     6841 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
     6901 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     6961 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgcaggaaa
     7021 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     7081 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     7141 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     7201 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     7261 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     7321 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     7381 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     7441 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     7501 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     7561 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     7621 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     7681 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     7741 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     7801 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     7861 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     7921 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     7981 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     8041 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     8101 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     8161 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     8221 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     8281 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     8341 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     8401 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     8461 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     8521 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     8581 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     8641 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     8701 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     8761 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     8821 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     8881 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     8941 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     9001 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     9061 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     9121 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     9181 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     9241 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     9301 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     9361 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     9421 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     9481 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     9541 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     9601 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     9661 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     9721 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     9781 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     9841 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     9901 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     9961 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
    10021 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
    10081 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
    10141 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
    10201 ccaaaagaaa aaatggtttt tttccatctt tt
//
LOCUS       DAHHPA010000093        10120 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.094, whole genome shotgun sequence.
ACCESSION   DAHHPA010000093 DAHHPA010000000
VERSION     DAHHPA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.094"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            109..615
                     /locus_tag="NBH55_002287"
     CDS             109..615
                     /locus_tag="NBH55_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCD1510233.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            632..1531
                     /locus_tag="NBH55_002288"
     CDS             632..1531
                     /locus_tag="NBH55_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCD1510234.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1618..2502
                     /locus_tag="NBH55_002289"
     CDS             1618..2502
                     /locus_tag="NBH55_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCD1510235.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2694..2765)
                     /locus_tag="NBH55_002290"
     tRNA            complement(2694..2765)
                     /locus_tag="NBH55_002290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2730..2732),aa:Arg,seq:ccg)
     gene            2825..4159
                     /gene="rpoN"
                     /locus_tag="NBH55_002291"
     CDS             2825..4159
                     /gene="rpoN"
                     /locus_tag="NBH55_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCD1510236.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQLNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4308..5399
                     /locus_tag="NBH55_002292"
     CDS             4308..5399
                     /locus_tag="NBH55_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCD1510237.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5440..6441
                     /gene="gap"
                     /locus_tag="NBH55_002293"
     CDS             5440..6441
                     /gene="gap"
                     /locus_tag="NBH55_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD1510238.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6551..7741
                     /locus_tag="NBH55_002294"
     CDS             6551..7741
                     /locus_tag="NBH55_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCD1510239.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7845..8600
                     /locus_tag="NBH55_002295"
     CDS             7845..8600
                     /locus_tag="NBH55_002295"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCD1510240.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8716..10014
                     /gene="eno"
                     /locus_tag="NBH55_002296"
     CDS             8716..10014
                     /gene="eno"
                     /locus_tag="NBH55_002296"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCD1510241.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3546 a         1865 c         2098 g         2611 t
ORIGIN      
        1 cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt
       61 ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact
      121 ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa
      181 actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc
      241 ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa agaaaaagag
      301 atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg
      361 cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta
      421 cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga cagtccggaa
      481 aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc
      541 ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt
      601 caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa
      661 actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca
      721 gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga gacgtctcca
      781 agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag
      841 tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt
      901 aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac
      961 acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg
     1021 acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca
     1081 agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca
     1141 gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa
     1201 aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc tatctgtttt
     1261 gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta
     1321 ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg ttacgcctac
     1381 acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat
     1441 tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat
     1501 tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca
     1561 attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg
     1621 aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt tttcgaaatt
     1681 acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg
     1741 tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct
     1801 aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc atcacaatca
     1861 acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc aatcagcttt
     1921 gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac
     1981 gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag
     2041 cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca gaatctggcc
     2101 actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa
     2161 gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac gaatgtagga
     2221 gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca
     2281 agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg ggcaattatt
     2341 ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg
     2401 aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc aacggctgtc
     2461 gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta
     2521 ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa
     2581 caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg gacagttttc
     2641 acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta gaacgcgtcc
     2701 tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca ctacactaag
     2761 gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg gattggacaa
     2821 aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga aactagccat
     2881 gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg agctgttggc
     2941 attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc cggaatggca
     3001 gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa attacctaaa
     3061 tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag tccatttgaa
     3121 ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca tcgatttgaa
     3181 cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta cgcctatcca
     3241 aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag gtgcacgtag
     3301 tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg aattagctta
     3361 tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag aaatcgcaca
     3421 acgtttttct gttgatctac atgctgttca gcagatattc gactatttac aaacgttgag
     3481 tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc ctgatgtccg
     3541 ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa atcagctaaa
     3601 tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata aagaaactga
     3661 aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga tcctacaacg
     3721 tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag ctttctttat
     3781 cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg agatagatgt
     3841 gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag acttcggtgt
     3901 atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag aacagacaga
     3961 agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa
     4021 aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg agatagcgat
     4081 ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt catcaaaaag
     4141 aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact ttttctagtc
     4201 tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt tgagatttcc
     4261 ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg ggtcgtttta
     4321 agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat gaaataaaaa
     4381 tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat aaaattttac
     4441 gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt atggggatca
     4501 cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg atcgacacct
     4561 caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc cttgaaaact
     4621 tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa tattttggta
     4681 accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat
     4741 tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag aatatcatcg
     4801 ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat ctggaaaata
     4861 agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg ataactgtac
     4921 aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac cgtgcacttt
     4981 atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag ccatctatcc
     5041 aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt gggcgtgcat
     5101 tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg aaacaagcaa
     5161 atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc gtttataagg
     5221 tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg
     5281 cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa
     5341 cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta accctttaaa
     5401 ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa agtaggtatt
     5461 aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt agatggaatc
     5521 gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt gttaaaatac
     5581 gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc attcaacgta
     5641 aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc atggggcgaa
     5701 ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc agcagctgaa
     5761 aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt
     5821 ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac agttatctca
     5881 ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa cgacaaattt
     5941 ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca aatgactcta
     6001 gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt
     6061 cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt gaacggtaaa
     6121 ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga attagtaaca
     6181 gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga agcttcaaac
     6241 gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg tatgacttac
     6301 ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa acaattagtt
     6361 aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt acgtacttta
     6421 gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata gacatgtaca
     6481 tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag tttattcagg
     6541 aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca aaaaagttct
     6601 tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg acactcgtat
     6661 caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag caatcctttt
     6721 ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt tagctccagt
     6781 tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg
     6841 agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa
     6901 tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag aactaggtaa
     6961 atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag ctcaccgtgc
     7021 acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg gattcttgat
     7081 ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac caatgattgc
     7141 aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact tgattcctaa
     7201 agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag caaaaggaat
     7261 caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag aattaatcga
     7321 aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa
     7381 tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg ctttagacat
     7441 cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa cagttgtttg
     7501 gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa tcggtgtttg
     7561 tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag actcagctgc
     7621 agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag gtggtggcgc
     7681 aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca acgataaata
     7741 aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg gagttttcat
     7801 gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt aaacctatta
     7861 tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt gctgaagcag
     7921 taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct cctgcattat
     7981 tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca gcacaaaact
     8041 gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct cttgctgatc
     8101 taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc catgaaacag
     8161 acgaagacat caataaaaaa gcaaaagcta tctttgcaaa tggtatgaca ccaatcctat
     8221 gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg atcgaaggac
     8281 aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg gttattgctt
     8341 atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc gctgacgaaa
     8401 tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta tcagaagcag
     8461 ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac atggcaaaag
     8521 aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca tttttgtcat
     8581 tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact
     8641 aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa aacaaactca
     8701 aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat cttagactca
     8761 cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt tggccgcgga
     8821 atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg tgacggagac
     8881 aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa caacatcatt
     8941 gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc
     9001 gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt aggtgtttct
     9061 atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca ctacctaggc
     9121 ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg cggatcacat
     9181 gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc tccaacattc
     9241 aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat cttgaaagct
     9301 cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct tggttcaaac
     9361 gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt acctggtaaa
     9421 gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga aaaaggtgtt
     9481 tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat caaattctac
     9541 gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga cgaaaacgac
     9601 tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt agttggtgac
     9661 gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac
     9721 tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga agctatcgaa
     9781 atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga aacagaagat
     9841 tcaacaatct ctgatatcgc tgttgcaaca aatgctggcc aaatcaaaac tggttctcta
     9901 tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga ccaacttggt
     9961 gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa ataattcttt
    10021 tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac ttagttaagt
    10081 aatcgaactc tctacttgct gaaatatcag caggtagaga
//
LOCUS       DAHHPA010000094        10099 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.095, whole genome shotgun sequence.
ACCESSION   DAHHPA010000094 DAHHPA010000000
VERSION     DAHHPA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.095"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(562..786)
                     /locus_tag="NBH55_002297"
     CDS             complement(562..786)
                     /locus_tag="NBH55_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510242.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            977..3754
                     /locus_tag="NBH55_002298"
     CDS             977..3754
                     /locus_tag="NBH55_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCD1510243.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3836..4255
                     /locus_tag="NBH55_002299"
     CDS             3836..4255
                     /locus_tag="NBH55_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCD1510244.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4267..4737
                     /locus_tag="NBH55_002300"
     CDS             4267..4737
                     /locus_tag="NBH55_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1510245.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4754..5533
                     /locus_tag="NBH55_002301"
     CDS             4754..5533
                     /locus_tag="NBH55_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1510246.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5520..6341
                     /locus_tag="NBH55_002302"
     CDS             5520..6341
                     /locus_tag="NBH55_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCD1510247.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6358..7407
                     /locus_tag="NBH55_002303"
     CDS             6358..7407
                     /locus_tag="NBH55_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCD1510248.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7420..8424
                     /locus_tag="NBH55_002304"
     CDS             7420..8424
                     /locus_tag="NBH55_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCD1510249.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9057..10076
                     /locus_tag="NBH55_002305"
     CDS             9057..10076
                     /locus_tag="NBH55_002305"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCD1510250.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3390 a         1659 c         2100 g         2950 t
ORIGIN      
        1 tttttctata aatgaattct gcataaaaaa tatatcaagt aaatgtttat agatgaaact
       61 ctatttactt cttttttaat ttgttcttgc aatctttttt gctacgtaat ttttgtaaaa
      121 aagtgggttg agagaaatct aattgatttt caaagaagga gtatgaaaat gagaaaaata
      181 tttattcgtt tattgtgggt actatcttag aggaataaat aatagtggag aaaactatgg
      241 tttggcgcag attctatcag gtttgatgga acgaagacaa cctcccgaca tcaagtcaaa
      301 agctacttga gattttgatc tgaaaataaa aaaagaaaac aaggtaggaa catcaatgtt
      361 tctaccttgt tttcgtaatc aatgggaaaa aactaagagt ggtgtacgta atcaataaaa
      421 aggatggtga caacagtacc tctttactct ctgaaagcat cataactcgt ttttcctcat
      481 tatgccaata aattgttgtt ataccacata taaggatttg taatagctaa aaacagacag
      541 gaaagttgaa atctgtgaat cttattttgt atggaaggat ttatagaacg caaaaatcaa
      601 gataaagatg aataaaaatc ctgtttcggc tatatattgc gtatctacgc caataaaacg
      661 agtttgttct gtcatagcca tataaagcaa aggcaaaaat acaactacag cgagataaga
      721 tccgatgatt tctcgaaaag ttttccagga agtatgtttc ctgtttttat atttatccga
      781 tttcattgaa tagtcatagc ctccttattc tttcatatgt tgaaattcca gtataaaaac
      841 tggaaggaac tgtacgtttt ttagaataaa aattataagg gataagtagg ctgctgtcta
      901 taataagata tatttttcaa ttctttatta aagattttat tgacaaaatg aatccgtttt
      961 ctatgatgga ggaggggtga ataagttgtt gaagaaggaa attttatgtt acttagagaa
     1021 ccagacagcc tttttcgatt tagaacatat gaacgaggtc tttacagcac aaaggttagc
     1081 tgatcatttt ggggtaaaaa gaaacacagt cagtcattat ttgaaccagt taaatgatga
     1141 gcaactacta gtcaaaatca attctcgacc agtgatttat ttccacaaag aagcttttga
     1201 aaaacaattt tttaagttac gcacgaattt ttatttttct atcgatgatc tcaaagagga
     1261 acagcctttt ttcgcgcaac caaaagactt attctctttt atgatcggat acgacgccag
     1321 tttgaaagaa agtatcgaac aaatcaaaac agctctttac tatccagatg gcggattacc
     1381 acttttgatt acaggcgaaa gtggcacagg taaaagttat ttagttaatt tggtctatca
     1441 gtattgtctg cttcatgatc tgctagaaga ttctgcacca ttcatcactt ttaattgtgc
     1501 acaatatgca gataatcctg aacttttgac tagcaatttg tttggacatg ttaagggagc
     1561 gtacactggt gcagaagaaa ataaaaaagg ggcttttgaa gcagcagatg gtgggatctt
     1621 gtttttagac gaggtacatc gcttaacacc agaaggtcag gaaaaactgt tcacctatct
     1681 tgatcaaggt gtgatttatc gtatgggaga acctaatttg tcacgccgga tcaaaacacg
     1741 tctctttttt gctacgactg aagagattac tagtcatttc ttaacaactt tcattcgaag
     1801 aatacctatc cgaatcgaac tacctgcact taatcagcgt agtcgtaatg aacggctaga
     1861 actgatttac tctttttttc tcgaagaaca aagaaaaatg gggagccctt tgtcagtcag
     1921 tggaccagct ttgtcacttt taacaagtca gaaattatcc ggaaacatag gagagctgaa
     1981 aaatatcgtc aaagtttcag cagcaaaagc atatgctgaa cagcgtgaac agcctgaaat
     2041 ccatgtcacg atccatcatt tgcaaaaaga actacttgcg cagcctatta ctaaaaatac
     2101 agcacagcaa ggaatctata ttacgcaaga ccagacctta gagcaattaa tggagaagca
     2161 acagcccgaa cagcagcgaa tcgttaaaag ttttgagcaa atcctaattg atttcaagaa
     2221 aaataactgt ctgctatatt cttgcgaagg aaaattaaag caagaagtga ttcaattatt
     2281 tgattttttg ctgtttgaga ccagtagaca agaaaaacac gagctgctgg tttattggac
     2341 acaatctatc cgagagacct ttcgacaaat ggaaactgcc tatcaaatca aattcgatgg
     2401 aaacagtgtc tatgccttga gttactatct ctatcagcga agcactatca aatggttacc
     2461 ggaagaacat gatattatcc aactaatcaa agaattagaa caacaagttt ctcagtctta
     2521 tccatctagt tatcactatg tccaacgaat tctggaatta agtaagatca aattagatat
     2581 tgaagacaca agtatggatc ggattttact gacgatctat ctaaaaaaag cgaaatggtc
     2641 aaaagaaaaa cgactgccaa aagcgatgat tgctgctcac ggttatgcaa ctgctagcag
     2701 tatggcaaat gtagtaaatc gtttattaca agaggatcta tttgaatcat ttgatatgcc
     2761 actagatgtc acaccacagc agattgcttc agaaatcctt gattattgcg aaaacagcga
     2821 tgtttcaaat ggattgatca tattagtaga catgggatct ctgaaagaaa tccatcagtt
     2881 ttttaaaaaa cagttatccg taccgctttt gattttgaat aatgtgacga cacctttagc
     2941 aatcacagta ggggaatgcc tgcaaaaaaa tgtatcatta gaagagatag cagaagagac
     3001 tgttcgacag atccagccag aatggcgact gctctatcca gaggaaaaca aacccaaagc
     3061 actcattact acttgcttca ctggaatcgg tacggcggtc cacttaagtg aactgctaga
     3121 aaaaagctta cctacggctt gtcagttgaa aatcatccca tatgaatatc agcaattaaa
     3181 agataagaaa aacagtgacc cattattttc catatacgaa gtcgtaggaa tgattggcac
     3241 cacagatcct gatatcacca atatcgacta tctttcatta gaagacctca tctcggggga
     3301 aaagatgtct gtactagctg aatggttgga cactactatg aatgcttcag agaaggaaat
     3361 attcaatcag cgcatcattc gtaatttttc attggaaaaa gtactggatt ccgtgactat
     3421 tctagataca gagaaggtga tgggagaaat cgatcatttt atgagagaat tagaggtgca
     3481 gttgaatcga agcatcagca atcccaaaaa attagcgttg tatgtccatg tgagctgtct
     3541 cattgaacga ttgatccgac aaatgccaat cgaaacctat caaggattag agaaatggaa
     3601 gcagtgtcaa aaagaagggt tatctgctat taaatctgcg tttagtgtca ttgaagagca
     3661 ttatagtgtc atgatcccca attctgagat tgcctatatc tatgatattt tgagtgaaga
     3721 tacagagttt ctctcgatag aagaagaatt ttgaacataa gcaagttgtt gccactaatt
     3781 ttggcatgca acttgctttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga
     3841 tagagaaatt atcttagctt cccatggaaa atttgcttca gggatattag attcattaga
     3901 gctgatctat gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt
     3961 tgatttagcg gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt
     4021 tctgacggat ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga
     4081 tcatgtctat ctcgttgccg gattgaattt gccactattg attgagctag tatctcggtt
     4141 ggacgctgaa gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat
     4201 gatccagttt tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga
     4261 agaaaaatga ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt
     4321 tcatggacac aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg
     4381 aatgagatac gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc
     4441 aaatcaattg atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta
     4501 ttcatcgtag tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc
     4561 aaaaccatca acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg
     4621 gtaaatattt taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa
     4681 gtagaaatcc gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa
     4741 aggaagaacc tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca
     4801 atcagaatat gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac
     4861 aggaatcgtg ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc
     4921 atttatcgga tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat
     4981 tttagggacg gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg
     5041 tttgccgatt gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc
     5101 aattatgaac cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca
     5161 tatgcacttg cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc
     5221 ttatatggtg ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca
     5281 aaaggggttg tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg
     5341 tttgctgatc aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta
     5401 cttagagatt cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt
     5461 caatattatg aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga
     5521 tgaagaattc tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt
     5581 ggcgttcttt tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg
     5641 tttatgcaat gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag
     5701 ctttgaaacg gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag
     5761 gaatcacggc tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga
     5821 tcgatagtat caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt
     5881 ggggaacttt gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata
     5941 ttttaggccc tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc
     6001 tagcaacagg ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg
     6061 gtatgatgga aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa
     6121 tgactgccac gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg
     6181 tgactgttca aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg
     6241 gcatagtgtt ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag
     6301 ctttctttgg aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg
     6361 tttacgatgc aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag
     6421 aatgattttt cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg
     6481 ggttcatctt ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt
     6541 actgtcgata ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc
     6601 gatactgtga ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa
     6661 atgatacaaa aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt
     6721 gctttagaag ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt
     6781 acaaaaggct attctgcgac tgttttacag ttactactct tagggatagg aattggaata
     6841 agtaaaaata aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata
     6901 atcaaccatt taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt
     6961 ttatttcgtt tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct
     7021 aaagaagcag agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta
     7081 gaagcttata tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt
     7141 gaaggggaaa gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac
     7201 gttggagaga ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta
     7261 gcaatagaat gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc
     7321 gcttataaaa ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt
     7381 gatacagtct tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa
     7441 gaacaaatca aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc
     7501 gataaacttt atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat
     7561 gcttcctcga tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta
     7621 cagcatgctg cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt
     7681 attttgtcct ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag
     7741 aaagtaggag cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta
     7801 tgcgattttg tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca
     7861 gcagatcgat tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag
     7921 ctcgaacgtt atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc
     7981 ttatcatttg cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat
     8041 caatggggag ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca
     8101 tcgaaatcta tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca
     8161 accccagtca cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct
     8221 aaacttttgc caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta
     8281 gaaggtatca gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg
     8341 acacaacgca tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc
     8401 tactatcgtc agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag
     8461 agcagcgata actgctccct ttattttttt aaaatattgg catcaattac gttataaaac
     8521 acattttttg cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc
     8581 aaccattttt tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc
     8641 caagtacaac gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac
     8701 aaaagttcct atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt
     8761 tagaaatgtt caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc
     8821 taacctccta atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa
     8881 gcaaagcata acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt
     8941 caagtaaaaa cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac
     9001 tttaagaaat gtatactata atggaaatta tcaattattt atttggagga atatccatga
     9061 aacaaaaaat cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg
     9121 cagaaaacgg gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca
     9181 ataagtatca tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg
     9241 gatataaaga attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa
     9301 caaaagcgat tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca
     9361 ttatacatgc aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg
     9421 ctgaagaaat tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg
     9481 ctgaggaagt ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag
     9541 cagcagccta tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg
     9601 atgtgattgg cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg
     9661 ctatccatgg attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg
     9721 cagaaatcag ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga
     9781 gtggtgtggg ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg
     9841 gtaacttgtt aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg
     9901 ttgagggtgt ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga
     9961 tgccaattac tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag
    10021 caaaagaaat catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag
    10081 atgatcaata gttaatcgt
//
LOCUS       DAHHPA010000095        10030 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.096, whole genome shotgun sequence.
ACCESSION   DAHHPA010000095 DAHHPA010000000
VERSION     DAHHPA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.096"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            140..460
                     /locus_tag="NBH55_002306"
     CDS             140..460
                     /locus_tag="NBH55_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510251.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="NBH55_002307"
     CDS             912..1112
                     /locus_tag="NBH55_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1510252.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="NBH55_002308"
     CDS             1259..4048
                     /locus_tag="NBH55_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCD1510253.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="NBH55_002309"
     CDS             4095..4622
                     /locus_tag="NBH55_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCD1510254.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="NBH55_002310"
     CDS             4643..5833
                     /locus_tag="NBH55_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCD1510255.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="NBH55_002311"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="NBH55_002311"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCD1510256.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="NBH55_002312"
     CDS             8224..8898
                     /locus_tag="NBH55_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCD1510257.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="NBH55_002313"
     CDS             8895..9236
                     /locus_tag="NBH55_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510258.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="NBH55_002314"
     CDS             9266..9625
                     /locus_tag="NBH55_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCD1510259.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
BASE COUNT         3345 a         1792 c         2148 g         2745 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa
//
LOCUS       DAHHPA010000096         9762 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.098, whole genome shotgun sequence.
ACCESSION   DAHHPA010000096 DAHHPA010000000
VERSION     DAHHPA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.098"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            118..312
                     /locus_tag="NBH55_002315"
     CDS             118..312
                     /locus_tag="NBH55_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCD1510260.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            315..1517
                     /locus_tag="NBH55_002316"
     CDS             315..1517
                     /locus_tag="NBH55_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1510261.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(2100..3731)
                     /locus_tag="NBH55_002317"
     CDS             complement(2100..3731)
                     /locus_tag="NBH55_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCD1510262.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3745..5418)
                     /locus_tag="NBH55_002318"
     CDS             complement(3745..5418)
                     /locus_tag="NBH55_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCD1510263.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5431..5748)
                     /locus_tag="NBH55_002319"
     CDS             complement(5431..5748)
                     /locus_tag="NBH55_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD1510264.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5773..7173)
                     /gene="lacG"
                     /locus_tag="NBH55_002320"
     CDS             complement(5773..7173)
                     /gene="lacG"
                     /locus_tag="NBH55_002320"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCD1510265.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7410..8195
                     /locus_tag="NBH55_002321"
     CDS             7410..8195
                     /locus_tag="NBH55_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1510266.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8198..8890
                     /locus_tag="NBH55_002322"
     CDS             8198..8890
                     /locus_tag="NBH55_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCD1510267.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9164..9535)
                     /locus_tag="NBH55_002323"
     CDS             complement(9164..9535)
                     /locus_tag="NBH55_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCD1510268.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3098 a         2064 c         1706 g         2894 t
ORIGIN      
        1 agttgggaag tcctgaacaa gtgatggatt ttcaagaaag taccgttcat tatacaaaaa
       61 taaagaaata acactattac ccaatgatag tgttttcttt gaaagtgagt ttatacaatg
      121 aaaaatacaa tttgtcgaaa agatttagag gctctaggat ttaaaacaca tcaagctacc
      181 acaataatcc gacaagcaaa aacaagaatg gtaaatcagg gatatccata ttataataat
      241 aaaagagttg gggtagtacc ccgacaagta attgaagaaa ttataggtgt tcctctttca
      301 caggaggaat aactatgaaa acaaaatacc cttgtgtgta tcaagataaa aatggtcgct
      361 tttcttatct agttgagtta ggaacagatc aagtgactgg aaagaggatc cagaagaaag
      421 gaacaaaaga ttctctagga aaacgattct catcagctcg tgaagcacac aaagaagtca
      481 tgcgtataaa aaatgaatac atggttgtaa atggatttgc gaattatgat ttaacttaca
      541 aagaatttat ggagcagtct tacgttccac actacaaagc tagcgtacaa aaaagtactt
      601 ggagttctcg tgagtgtgga ttgattcaga ttactgaatt tttcggtgat aagaagcttc
      661 gtgaaatcaa tgtgcgagat tgtgaaaatt atcgaatttg gttattgaac gatagcggat
      721 actcacaagc atacagttca cttatgtacg gtatgtttcg taaatcatta gactatgcgg
      781 tcaccctgca atttctgaat gagaatattt ccaaaagaac aaaagcaatt ccaaaaggaa
      841 aatcaatcgt tccttactgg acaaaagaag aatttgaaga agttttggca aaaatttata
      901 aagatgattt ctacgaacat atgtgttttt taatgatctg gctatattac atgactggtg
      961 ttcgtgtaag cgaaggatta gcacttaatt ggaatgacgt ggacttcaaa cgtaaaaagt
     1021 tgagggttca tcacaccttg gaaatgacaa atcaaaaaga ttttattcgg aaaccttaca
     1081 caaaaactga aagcggcttg cggatcattt ctctggacga tgatacaatt gaagtgctaa
     1141 aaaagtggaa agttattcaa aaaaatcatg gtgtagaaca atttattctt agctacactg
     1201 acctcccact ctatcgttca actgttcaac gaattattca gcgttatgcc aaactagcta
     1261 aggtacccct aatacaagga aaaggattac gtcattctca tgttagctac ctaatcaatg
     1321 agtttaacgc agacattctg gtcgtgtcgc aacggttagg ccattctagc cctgaaatca
     1381 ctttaaagca ctacgctcat ctatggagcc gtaatgatga aggaattgca gaacaaatga
     1441 caggaaacat taaatttacc tgtgcaacaa aatctaaagt tgcaaacttt aatggaaatc
     1501 aagcagtaaa aatctaaccc taccatttcc ctaccaaaat gaggaatagg ctgaaaaagc
     1561 actgatataa tagcttcttt acgaggctac tcatattgag tgggaataaa gagccaacat
     1621 aatatggagg gggaatccct ataaatcaac attcttcaat agctaaaatg cttgattttc
     1681 attaaaaata agatggttcc ctaccaaaag agactataat ataagttaat ttgtttcatt
     1741 cttatcataa aaagaagttc tcgaaaaacg aactgttttc cgagaacttt tttctatcca
     1801 tctaagtgct caaatcatat tcacgacgga attctaaggt taccctttct taaacaaact
     1861 ttatgttatc tgcacaactt tttgcaattt tttcattgct tcttacgaaa tcgttgaact
     1921 ctctttagca taatacaaag tgtagatgaa gtctggtgct ttactcgttt tggtaaaaat
     1981 gactggaaat cgtttctggt tatttttggc aaaggcgagg tgcaggttcg attcctgcct
     2041 tctacgctaa cgatgtggct gaagggaacc tccgatcttg aatcgaaacg atccgcagtc
     2101 tattgtttca ccaatgaaac aaaatcaacg ttcgtccaag catttcctga agccttgcta
     2161 taaaccccaa tttcacaaac acctgttgta attggaatgt ctttgattgt aatttttgtc
     2221 cattcacttg ttttaacaac cgcagtattt agttccgctc caccataatt tttgatatat
     2281 aactgattag tatcaaactc tcccccagac attacatagg cactcaatgt gtaagttcct
     2341 ttgcttatgc cactaacatt ttgataagct gatgcctcgt atgcttgatc ctgccaaaaa
     2401 gttagtttgt agtccccaaa aaaagtgccc gcttccgttt tgatcgtacc cttagcggtt
     2461 ccttgttgaa accaggtttg ccaattggaa ggactggtcg ttggtccatc gctttcgaaa
     2521 cttgcatttt taactagatt tatttgcgcc cctgcttgtt taaacgcacg caagcttggc
     2581 aaggcgtttc cagcaaaatc aaacatcgct tggttgtccc attcgtttcc catagtggat
     2641 tgaacactga gataattcat acctgcttgt gttgcccaac tcacatttcc gttgtaccaa
     2701 agaggttccc aatagaagaa gcccgccccg cgattattcg gtactgcctg aatccgatcg
     2761 gcaagatcca gcaaatactc tgcttgtcct gtcggtgttg gttcatatcc ccccgcagct
     2821 gcttcggctt caccaaaagc atttggcata gtatcggcat tcgctgttgt atgcccataa
     2881 gctgtttcaa ctacgatcac ttctttacca taccgttggc tgatcgcatt catattggtt
     2941 tgaagttcat tcattgaacc gtgccaatac ggataatacg acactccaat taaatcaaat
     3001 gacacacctc gactcgtgat ttcatcaaac caccaagtaa atgctcctgt atcgccacca
     3061 tcagctaagt ggagcattac tggagttgtt ttaggttgag tgtctcgaac agcttggata
     3121 ccagacttca aaaaagcagc caatcgatca aattctcccc cgccttctcc ccaagacttg
     3181 ccataaggcc agagcattcc gctattcagt tcattgccga tctgaaccat atcaggataa
     3241 acgtccagct gcttcatttc cgacagaacg ttctttgtat agtcgtacaa tgccgtattc
     3301 atttcttcaa aggtcagacc ttgccatgct ttaggaagat tctgtttccc aggatcaacc
     3361 caaaaatcac tgtagtgaaa atccagcagc accttaagtc ccagattttt tgctcgtttt
     3421 gcaaggtcaa tcgtcgtttc cagatcattt gtccctgctc cataactttc tccattacta
     3481 ttataaggat cctgccacaa acgcagtcga acatagttga caccattatt ttttaaaatc
     3541 tctaaggcat cttttgaaat atcgtcttca taatattttg caccagattt ttccatatct
     3601 gacaggatcg aaatatctgc tccgcgaata aattcgctgg cagatgcttc aacagttttt
     3661 gaaacagccc ccaaagcccc taacacagct gcaaataaaa cagctttacg caaccaaatt
     3721 cccattttca ttatggctcc tcctttattg aaattggttc agaataaact gaacagcttt
     3781 ttcaggatct ctcgttaatc cgatatattc tgctcctttt gtcgcaacca gcttgattcc
     3841 taagcgatcg gtgtcttctt tgatatcatc atagtaagaa ttgacttgcg gtgctaagac
     3901 caccatatca aaatcctgca gcatttcgta atgagaacca taagcacccg ccattgcact
     3961 gatcggtgct ttcgattcag atgcaccttt ttttaaagca ttagctaaca tcgcacttgt
     4021 ccctgcgcca gcacaaagta ccaacacatt gatttcttta tttgcatcgc tgataacttg
     4081 actcgctgcc acaggtactt cctcacgtac atcggcagaa gcaccttcct cggttgcgat
     4141 cacctctgct ttttgtgctt ctttctctac taagacagca tcgtatgctt tgcaaaatgg
     4201 tagatagatc aggaagtcaa cgacgattag aaccgctgct agaacaaaag ctaagatacc
     4261 catgcccgtt cctagaacca aaccgatcgg tgctggagtt gcccaaggca aaacgtacat
     4321 aaagctgttc atgcccaata catcgacaaa aaacttgaac aggcaaacgt ttacaacagg
     4381 tgttaacaaa aatggaataa aaaaataagg gttcaaaatc atgggtgccg caaatagcaa
     4441 tggttcgttg acagcgaaac ttactggaat aaaggaagct ttcccaactg ccttcaactg
     4501 cttagactta gcaaagagta ggaagataaa aggaacaacc aatgttgccc cagttcctcc
     4561 catagttcct acaaaattcc ctaaacccac cgtcagtact ttagaagctt gttcaccgga
     4621 attgaacaat tgcagattgg tttccacatt ggcatagata attgccgcaa ttgccggctc
     4681 cacgatcgac ggtccatgaa caccgacaaa ccaaaacagc gccatcgctc cccagataat
     4741 agcgattcct aagtacccgt cagctgctgt aaatagcggc tgcagcaccg taatgattgc
     4801 ttctgcaaat gagatttgga agatttgtcg aatgcctaaa tcaattaaaa cggccgcaaa
     4861 tactgaaaaa gagaaaggaa aaatatctcg gaaggtttgc gaaatcgttc caggaacttc
     4921 tttaggcatt ttaatggtaa tatctttcag cacacaaaac ttatataaat taactgttaa
     4981 aaatgcagcg acaaaggaag ccagtagtcc ctttgtcccc atatattccg ttgaaaatcc
     5041 accttcaatc ggagagacgc ttaacaacag gaaacttacc acagaggcca gcatcacaga
     5101 ggtggtgttg attactttgt ttttcggtaa gcgacgattc atcgaatcag ccaagcagcg
     5161 agcagtggtt gctgaaacga gtaaccctac aacacccatc gaataattat agactttcca
     5221 tagccatgtg gagacagatt caggccattg gtaaccaaaa atctcaggaa ccgcagcaat
     5281 caaaatgaaa atgcttgaaa ataaaatagc tggcatcgct gttaaaaatc cgtcacgaac
     5341 ggctcctaaa taactattac gtgagatttt ttcaaaaaat ggtttccctt tttcgatttg
     5401 tttaatgatt ccatccatag cgttcttctc ctatccttga ttctgtctgt acagtacaat
     5461 aaagtcttga atcagatctc tcagcaataa agtggtcatt aaatgatcct gcccatggat
     5521 aaaaatgaat cccatctcca tctctttccc agatgcttct tccgctaaaa tttttgtttg
     5581 cgaattatgt gcaagggtta aattttcgtc ggcttctttc aacgccgaat caacatgttc
     5641 aaagtttcct tgtcttactt ctttcaataa tttcaataat gtactgcgag cgtcgcctga
     5701 atatgcaacg atctcaaaac caagcatttg taattcctct ttattcatct tttcttcctc
     5761 cattatcact tattaaataa tcgttttggt ttcactgacc aacttgtacc agtaagctga
     5821 ttcttttggg taacgcttct gggtttcaaa atcaacataa aacaagccat accttttgtt
     5881 atacccattt gtccatgaga aaagatccat aagcgaccac aaaaaatacc ccttcacatt
     5941 taccccggct gttatcgcct tgcttaagga ttccaaatag actctcaaat agtcgattct
     6001 cggctgatcc ataataatgc catcttcaaa ctgatcttta taccccatcc cgttctcagt
     6061 gatatagatt ttgttgtaat gtgggtaatc gcttttaatt cgcagcaaca agtcatatag
     6121 accttctgga taaatcagcc agtcccaatc agttcgagga attccttctt tataaatacg
     6181 ttctccgatt cccttcactt tataaacaga ggtccctttt tcacccgtac cgttatgatg
     6241 gattgcattt tctccatcgt aagctttgac aaaatggcat tggtagtgat tgatccctaa
     6301 gtagtcatta cgcgtggatg cttttttcaa ttcaacgaaa tcttcttcag gaaaatgata
     6361 gctggcctga tttgcttcac atatctcatc aagagcagtc aaggtttcag ttgaataata
     6421 gcctaaatag gtagcatcta ataagaaacg gatcgacaat gcatcgtcta aaaaggcagc
     6481 atgcttatct tccggcgcgt ctgttgccgc atatttcgtc tctaatgagt gaactacacc
     6541 aatttcaccc ggaagttcgt tttctttgaa atagttcact acacgggcat gagcaaccat
     6601 catattatga agacaggcga cgatctttgt aaaatcatat ttgattcctg gtgggaaaac
     6661 acctaacaaa tattgattgg ttgcaactgg atagatttca ttaaaggtac tccacacctt
     6721 gacttctttg aattcatgaa aacaaaaaat ggcataggaa acaaatgctt ctattgtttc
     6781 tcgattcaaa aaatctccat ggtcaaacaa acgtttaggg gtatcaaaat gatgcaacgt
     6841 cacaaacggc gttacgtttc ttttcttaca ttcttcgaac acacgatgat aaaaagcgat
     6901 tccttctgga tttggtttcc cagctccatc aggaaaaatg cggctccaag caatggataa
     6961 tcttagacca tttacaccaa aacgttcaca caattcaatg tcctttgcat attgatgata
     7021 aaaatcactt gccggatcgg gactgaatcg cccttgttcc tctaaaaagt catcccaagc
     7081 aacagcgcct ttgccacctt ccttagtggc tccttcaacc tgataggctg ctgtggctcc
     7141 gccaaaaata aaatcttctg gtaatttaag catgtacatc tctcctcaaa tctataatca
     7201 gttttcgctc tttctaactg attgcattgt aaacgcatcc aaaacatttg tcaacaaaaa
     7261 caaacacaaa taatcataaa aaccagaaaa cagcaataaa atctattgta acaaacataa
     7321 aaaccaccct aaatgtttac ttttgttcga attagattga ctgttgtccg tttatatgat
     7381 agattatcct taaattagag gtgaagacaa tgttgaaaaa agaaagattg cttactatcg
     7441 tagaaatggt caataaaaaa ggaatcctta ccgttaatga aatcatcaat caattagatg
     7501 tttctgatat gaccgttcgc agagatttag atgaattaga aaaatcgggc aagctgctgc
     7561 gtgtccatgg aggggctcaa agtctttcct acactttaga tcaagaactt tctcacactg
     7621 aaaaagccac tctgcagatt caagaaaaaa aagaaattgc tgccacagca gcctccttga
     7681 ttcacgaggg agaaacgatt ttcttaggac caggtacaac aatcgaacta ttggcgacgc
     7741 atttactgaa caagaaaatt cggatcatta caaataatta tccagttttt gatattcttg
     7801 ctcaagcaga tggaccagaa attctactga ttggcggtga tttccggaaa aataccggcg
     7861 catttgtcgg accgatcacc aacgacaatt taagaagact aaagtttaca aaagcattta
     7921 tcagttcaaa tggcgttcac aacgaagcga tctctactta cagtaccgaa gaaggagaag
     7981 cacagcagat cgctctaaat aattcccgta agaaatatct actcgttgat aataaaaaat
     8041 tcaatcgaga tgatttttat gtgttttata atttgcatga tttcgatctg ctcattacgg
     8101 atcgccagat ctcgcgggag gttcaagagc actatgcaca atatgtcgat ctgcaaattg
     8161 ctgattcaac cgaaaatacc actataggag cgtaactatg aaagggatta tatttgattt
     8221 caacggaaca atgtttttgg actctcatct gcatgaagct gcttggctgc atatgataca
     8281 tgatcataca aaagatggtt tatcagacga ggatatttta aaaaatattc atggtcggac
     8341 gaatacggag attttgaaac atttcatttc agagcatttg accgcctctg agatcgccgt
     8401 attgtcagaa gaaaaagaaa gctattatcg tatgctttgc ttgcaaaatg aaaacgagct
     8461 tcagttgaca aaagggcttg aagcgactct tgaccagctg actgaccaac agattccatt
     8521 aacgattgcc acggctactg tcaaagagaa tgttgctttt tattttgata ttttcgattt
     8581 agcccgctgg tttgactggg acaagattgt ctatgacgac ggctctttcc ctgggaaacc
     8641 gcaaccggat atttttctta aagcggcaga caaactgagc ttagctccgg ctgattgtct
     8701 tgttatcgaa gatgcttact caggattact tgctgccaaa cgtgcgggga tcggaacaat
     8761 tattgcaatt gatccattcg gtaaaaacaa aacagtattt gaacaggcac agctaaataa
     8821 agatggaacc atcaaagatt ttactggttt ttggacagag cacctgtctg ccacatccgt
     8881 taattcttaa acgattcaac taactaccaa agaaaagcaa ctgaaatgca cacctccttt
     8941 tataaaaaac tgccagtgaa acgacatgaa agatacactg ctatatcctt agtgaaatat
     9001 agcagtgtat ctttgacttt ttcattcttt taaccatatg acgcgttcat ctctatctat
     9061 aattaacatt aaaaatagtg ttttcacatt atttctcaaa ccaatcttaa aaatcatcag
     9121 gagtactttt ttgatatact tttattaatg ctgggcgtaa tggttaaaag atttccacta
     9181 gttcctctcc accaagctga gttgaaaaat gcttatctgt aaacaacata gacaaagagg
     9241 aaataaacgt ttccgtatct ttctctgtcc caaggatatt aattaatttt cctactaggt
     9301 aagtatcatt aaacttaaga tctgccttca catcaaaagt tttgataaaa gcttttatca
     9361 tgcgtacaat tttgtcttta tcgccataat tgatttcacc actcatatca atatagtcac
     9421 taatccctga agaaataatg gcaactgctt cttctttcgc cttatcagat aattcattta
     9481 atgactggga ttgattgatg acttcttgag ttttatctgt tacgtaatct ctcattatcc
     9541 tatcggcata ctccgcaaag taatcttgaa aaaattcttc tacagggtaa ccaacttcgt
     9601 tttcccaacc aggagaaatt tcattatcag tacttattaa atctattaaa aaccaacccg
     9661 ttctctgagt gttgattcta taatattttt aatttcctat ataaatcaaa aatattatag
     9721 aatcaataag ctaattaaga aacaaacttt agaaactcta ga
//
LOCUS       DAHHPA010000097         9726 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.099, whole genome shotgun sequence.
ACCESSION   DAHHPA010000097 DAHHPA010000000
VERSION     DAHHPA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.099"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            51..3329
                     /locus_tag="NBH55_002324"
     CDS             51..3329
                     /locus_tag="NBH55_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCD1510269.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3870..5777)
                     /locus_tag="NBH55_002325"
     CDS             complement(3870..5777)
                     /locus_tag="NBH55_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCD1510270.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5907..6782
                     /locus_tag="NBH55_002326"
     CDS             5907..6782
                     /locus_tag="NBH55_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCD1510271.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6862..7836
                     /locus_tag="NBH55_002327"
     CDS             6862..7836
                     /locus_tag="NBH55_002327"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCD1510272.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8016..9161
                     /locus_tag="NBH55_002328"
     CDS             8016..9161
                     /locus_tag="NBH55_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1510273.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9298..9642
                     /locus_tag="NBH55_002329"
     CDS             9298..9642
                     /locus_tag="NBH55_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCD1510274.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3167 a         1801 c         2060 g         2698 t
ORIGIN      
        1 aggtaatact ttaagtaaac gctttctaat ttgttacggg aggaatacac ttgttaaaaa
       61 aacgtataaa atacagtgcg ctaatgatga atctccttat gctcagtacc ccggttttaa
      121 tttctgtcgt tgaagttgct gctaacgaac aacagtctgt gaacgaagaa aatgcacacg
      181 tgaacattga agggatgtca acaatggagg aaataccaag aactagtgaa gatatggata
      241 cggaagaagg aaatgatcct atgatgaaag aatcagaaaa tgaaaagaca aaaaagcaat
      301 ctgaagaagc aaatgaagag tggaaagggg ttgttttcgg agaaagcaca agtgctagta
      361 ggtatgccat tgaaccatta gaagacggcg tgcggctctc ttccacgaat aacggcggaa
      421 aattccagtc aagcggatca gatggaatga cctattatta tcaagcaatc cccaaagaca
      481 tcaactttag gatgcgggcg acgatcgaaa tagaatcttg gacctatacg aatgcccaag
      541 aaggatttgc tttaatggta agagacgcag taccaaaaaa tcatttgttt gggcaggcat
      601 tttattcaaa ttcatttgcc atactaggca gtcggatcga atatgtctgg gactcggata
      661 gacaagaagt ggtcaacacg agcggcagca aatatacgat gcgacttgga ttggggacac
      721 gggcaatcac aggaatatca tctgaagatc cgcaagctgc gccagctcct gggagtgtct
      781 cagtggaaac aacaccttta gagacttcag cagggttctt ggaaaaagaa acaggttctt
      841 ataatatatt cggaaacggt catgggaatc tgtttccggc cacgaactcg attactaaac
      901 tggacgtaga gatgaaaaaa acgaatactg gtttagaggc atattattac gatcctgaaa
      961 caggagaaga aatggcttct gacatcatgt atgactggga aaaattatac gcttcagatg
     1021 aatcggttat ttatgcagga tttgctgcag caaggaatat gacgatcaag gtagaaggaa
     1081 tcgaagttgc ttactcagat cctgcgacag atcctcctgg gcaagaaaga cctactcgct
     1141 tgattgatcc gatttacact gtgacctcta gtaatcattc aggaaaccaa gatcatacta
     1201 tgtcctttag agcaaatgct gacggattat tgacaattaa aaataaagca acaggagagg
     1261 tgcttaagaa cgcaaaaaac ttggctatca ctgcgaatcg ggaatttgat tatcagacaa
     1321 aactgatgga aggaacgaac gaatttactt tcagttttac gccagataaa aactatccgc
     1381 cagaagaata cgtagagatg actagttatg aaacaaaaga gattcttcat acagtggact
     1441 accgtaaacc taaatattcg acagtttatg taacaccaga agggtcagat gcaggaaacg
     1501 gaagcagaca aaatccatta tctctgacac aagcattgag atacgcatat gctgggcaga
     1561 caatcgtcat ggcgcctgga acttattcct tcgaaagagg attgacgatt ccaaaaggcg
     1621 ttaacgggac aagagaaaac ccgattcagt tgattgcaga gaaaaatcca gaaaacaagc
     1681 gtcctgtact tgattttaaa ggaactggaa atgggatgac actgttcagt cactactggg
     1741 gtttacgagg gtttgacatt acaaatagtg cagctatgca aaaaggatta cagatttctg
     1801 gaaatcataa tttgatcgaa gagattcatg cttatcggaa tggaaataca ggaatccaaa
     1861 tcagcggtag ctcaaatgat cctttcgaag catggcccgc gcataactta gtcaagaact
     1921 gtacatcttt tgaaaatgcc gatccaggat ttgaagatgc cgatggattt gcggccaaac
     1981 tgacaatagg agaaggaaat gtatttgacg gttgtatcgc gtatcataat gcagatgacg
     2041 gttgggacct ttttgctaaa aatgaaacag gttcgatcgg aaaagtaatc atcaaaaact
     2101 cagttgctta tcgaaacggc tgggtaccag gaattgaggg agaaggtaac ggtaatggat
     2161 tcaaaatggg cggttcttct ttaacgggcc ctcatgaatt gatcaatagt ttgtctttcg
     2221 aaaatcttgc gaaaggaatc gattccaata gcggaacaga tattatcgtc aatagcagta
     2281 caagttttaa taacggttca tataatgtgg ctctttatac ctcttcagca ggaaacacag
     2341 actaccatgc ttctggtatc ttatctttcc gaacagagaa tcttgagatg agagagcaga
     2401 tccgcccatt gaatcaaaac gaagatcaaa tctaccactc gttgaactac tattggaatg
     2461 aacgagagaa acaatcagaa aatactcttg gacataccat atcaaaagat tggatcgagt
     2521 ctctaaatac atgttcgaaa gaaggtcagc agcctgttac gcgctatgaa aatggaagta
     2581 tccatgttca tggactgatg cagattaaac cggggaatcg tcaaacagaa aaggaaagag
     2641 tggatggtct tccattatct gtaggtgcta tttttgacgg agaaacctca ccgtcttcgg
     2701 aatatccaga gtatgcaatc gtacaagaac caaatcagac aaaaccaaat gaacaaaata
     2761 atccgattat tatacaagaa gcaaatggtg tggcggagaa tgcagaagga aatatcattg
     2821 aactgaagcc ttttacagtt tttgtgtcaa ctttcacacc aaatcaagga acgcctgtct
     2881 ttgaagatat gcgttatttc ccagaaaaga tccaaatcgt tgaaacacag tcaagtgctc
     2941 aattttctaa tcataccgct attttttcag caccttcaaa aggagaatat acgctagaag
     3001 tcacttatcg ttcggaaata tttgatgggg aaaaatggtc tgctcataca gaatctgtta
     3061 cccagcaaaa agcgatcact gtgcgagaaa ctgccgatgt gcccccggga aatgacaaac
     3121 cagaaagaga aaatccagga aatgaatcac caaatggaaa accaaatgga aaaccttctc
     3181 atccggatag gacagcaaaa gaaagtgaaa aaaatggcac tcaaggactc ccaaaaacgg
     3241 gagaatttaa taacccgttg ctagcattag ctggaggaat tttgttaatc ggtgtagttg
     3301 tttatgtcat gaaacagcga aaaaaataag tggaatttct ccttttatga tgtaaaacaa
     3361 ttgactgagg aaagtaaaaa tagaaggaaa tgagaccaat agatgtctca cttccttcta
     3421 tctttttata gatgaggttc tccttgctgc ttttgattgg ctgttcttat tacttgatgc
     3481 aggacagctt tttcacaatc tctccactaa acggtgttca atcagctgcc cctcggtatt
     3541 agctcaacaa acggcaaaat atgaggaact atttttccgt ttttttctct aatactcagg
     3601 tcaggacgct gatttcacaa cctctcgata aacggtgttc aaaagccagt ttctcggtag
     3661 taagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttactacttg
     3721 aaactaagca gctttttcac aacctctaat acatggtatt ccagaagtaa cttcttcgaa
     3781 aaataagccg aaatgtcaca aaaatttgag aagcgatttt cccaaattcc ttcttatttc
     3841 ttgaagctga ccacttctgg cacaacctct taccagattc gcactctttt ttctggcgct
     3901 agatacatag cgtcttgttc cgtgatacca aatgtttcat agaattcgtc aaggtttttt
     3961 ggttgcacgt ttgctcggag tttagcaggg gcatggacat cgatttgcag taaaagttgt
     4021 tggtattctt tttttgcttt tgtacgccaa atcgttgccc aattgacaaa gaattcctcg
     4081 atagaaagat ctgattccgt ttttgctgct tccaatgcac agcttagacc gcctgcatca
     4141 gcaatatttt ccgaaacggt taattttccg tttacttttc cgtcagcaaa aggcagacca
     4201 tcgaattctt cgatcattgc ttgtgctttg ttttgaaatt gtgtaagatc ttcttctgtc
     4261 caccagttgt tcaagttgcc atattcgtca aataaagcgc cgttattatc aaatgcatgg
     4321 gaaatctcat gagcgataac agctccgatt ccaccataat tagcagagct tgattgttct
     4381 agactgtaaa atggtgcttg aaggatcgct gccggaaaga cgatcacatt ccgaaatgga
     4441 tgataataag cattgaccgt gtcagcactc atttcccatt ctgaacgatc cactggtttg
     4501 ttccatttgc tgaaacgatc ttttaatgtg agtttgctga aaacaagagt atttgaaagc
     4561 aaagtaccac cctgatctgc aggtactgtt ttgaactggt cgaataatgc tgggatctta
     4621 tcggggtagc ctacttggat acctaagtta tttaatttta tgacggcttt ttcttttgtt
     4681 gcatccccaa gccagtcatt ttcttctaga cgttttttat aaacggcgat catttttttc
     4741 accatttgtt ctacgtcttt tttagcagct tcgccaaaat atttttttcc gtagtaatcg
     4801 ccaactactt gatcgaattg tccagaggca aggtaatacg ctgctttttt ctgaggcatt
     4861 gcttcgtctg ttccagacaa tacgcgagaa tagattccgc tgacttggcg gaattcttct
     4921 gatagataac cactaagaga acgtactgtt ttaatgatca tccagctttt gaataacgga
     4981 aaatgagctt ctgtcaggat gtcattcaaa tgatcgaagt acactggatc agttacgatc
     5041 actttatctg gagttgtccc aatcaaaccc gtgacaagtt ttttcaagtc cagtttatct
     5101 gtgtatttaa taaatgtatc aaaagattgc ggattataca ttttgctgta atctgcgttt
     5161 tcttctgcac tttttacatg aggagcaatc aatttatcaa attgcactgc ttgttccgtg
     5221 attgcttctg cctgactttt ttctttgccg gcaagctcta ataactgaac agccatatca
     5281 aagaaaacgc ctattagttg tgcaccgttg ggatggtctg atgcataata cgtcttatct
     5341 ggaaggaata aagaaggcgg ataagcaaag aaggcgttcg tttgcgcatt cttcatatct
     5401 gcatctacat ccagattaaa tggcaatggt aatgtatcca acagccattc tggaaattgc
     5461 ttattcaatt cgtcgaaaga ctgcaatgat tcaacttttt cgattatagg ttgaagcggt
     5521 tgagcatgca actcatttct tcggtcataa tcgtttgcca gttgataata tgcaaggaaa
     5581 tgtttcattt tttctgaaga cactttttct ggatgggcaa gcagatcgtc tgtttctccc
     5641 atcaaaagtt ggtcgatccc gtcaactaaa tcttggaaac cgcctgtagc tggtttatct
     5701 gcagggattt ttgctgtttt ttgccattct tcattgactg cttcatagaa atcttgtttc
     5761 aatcgttctt tgttcattca tcgatcgctc cttttttgtt tcgtgtgccc attatcttac
     5821 aaaatgaagg aattaacaaa tagaacataa aaaattgaaa attcgaacaa tcaaactgat
     5881 ttgttttttt caatccttca cttataatga aaaaaaggag gcgatcccaa ctgaatcaag
     5941 tagtggataa acctttatat ttcaaagtag ataaaaaaat taaaaaatta gccagcactc
     6001 agcagctgca gtcaagaaaa agtaaattgc tttttttagc tctggttttt gaagatcaaa
     6061 gttatgtgat tatcgatcaa tcaggacatc cggtagaata ttctccagca aaatatactt
     6121 atcaagaggg tctttcttgt aaaaaatgga agctgatcaa tgaagagcca atcgagttat
     6181 ctagatggat caacagaaaa gaagatattc ccgttttgat cgaagaaaaa aggtcaggaa
     6241 aggaactcgc aaattgctgg gtgggattgc cagaagaacg ttttttgagg tataagaaat
     6301 gggctacccc ctcaggctat ctttgtggta cttacgcggc agcagttctc cttgcctatt
     6361 atcaagacta tcgtaagggg tggatgcttc caaatgagat tcgcaagaaa aatacggctg
     6421 attcgttggt tttgacgaag gcattgcgta gccagatcca accactagga cttcctacga
     6481 ttccttttca ggttagtaca gggatttcta gttttttaaa gaagaatggc aatcatgagc
     6541 gtgcacgagc aactcttcta ggttcatggc agcgagctac aaaacgtata cgtgaaggca
     6601 aacctgtgat gattggtatt ttgaaagtac ttggcagtac atatggcaat cattgggtga
     6661 cagcgtacgc ttattttgaa actgaaacag gagaacgtta ttacaaagta cacgacaact
     6721 ggggcgatta tcataaagtc attcctgcca gttggagtaa cggaacagtt tcacttcctt
     6781 aagaatttgc tgaaattaac agaaaatctt ggaaaaatat gttaaactat cgaagaatag
     6841 agaagttttg gggaggacta catgagtaat aagtatcagg atgacacgtt gaagattttt
     6901 agtttgaatg gcaaccgtcc attagctgaa aaaatcgcga aagtgttcgg aacagaatta
     6961 ggtaaaagcg tcgtgaagca atttagtgat ggcgagattt ccatcaatat cgaagaaagt
     7021 attcgagggg atcatgtgta tatcgttcaa tcaacgaatg cacctgtcaa tgattactat
     7081 atggaactac tgatcatgat cgatgcaatg aagcgagcaa gtgcgaaaac gatcaatgtc
     7141 gtattgcctt actatgggta tgctaggcaa gaccgcacgg cgaaacctca tgaaccgatc
     7201 actgcgaaat tgatcgctaa cttgatcgaa gaagcagggg caactcgagt actgacttta
     7261 gatctgcaca cggttcaagt acaaggattc tttgatattc ctgtagataa tttatttacg
     7321 atgccgttgt ttgctcatta ttaccgtcaa ttaggtctga cgggagatga catcgtagtt
     7381 gtttcgccaa aaaacagcgg tgttcagcgt gcacgtagtc tttcagaata tttgaacagc
     7441 acattagcca tcgtagatca tgcagatgat gcttcaactg agggtggtta tgtgatcggt
     7501 gatgttcaag gaaagacatg tatcatggta gatgatatct tgaacacagg agcaacgctt
     7561 gcacgagcag caaatgtatt aaaagaaaac ggcgcaaaag aagtatatgc ctgtgcgtca
     7621 catggattat tgtccgatca tgcgaaaagt attttagaca atgcaccaat caaagatatt
     7681 tgtatcactg attctgtttt cacagaagaa aatcgtcatc cagaaaactt aacaatcgtt
     7741 acatgttcag aattaatggg tgaagcggtc aaacgcatcc atgaaaatac accgatgagt
     7801 ccgttattcc gtctggaaga aaaagaattc gaataaacaa aaaaaagagg gaaagaagtt
     7861 gtgacagaag tgagcagctt caaggagctg cgacaagact tttgtcacag ctcctttttt
     7921 ctatgcccat gaaacgtcct ctagacaaaa atgagctctt ctttggtttg ttcgctattt
     7981 tatgctagaa tgtgcctagc aataatttgg aagggatgaa tgaagtgaaa actatttatc
     8041 tggatcatgc ggcaactacc cccatgcatc cacaagtaat tgaagcgatg acggcaatca
     8101 tgcaagacac gtttggaaat ccttcaagca tacatggatt tggtcgccac gcgcatgaaa
     8161 aattggaaac agcaagacaa atcattgcag acagtctgca agcaaaacct cacgagatca
     8221 tctttaacag tgggggaaca gaaggggata atacagctat cttagaaaca gcgttctcgc
     8281 gtcaaaaaga aggcagacat ctaatcacta cagctatcga acatcctgct gttcttaata
     8341 ccatgaaata tttagagaca aaggggtttg acgttacata tctgcctgta gatgaaaaag
     8401 ggaatctgtc gatcgaagag gtcaaaaaag cgttacgaga agatacgatc ttggtctctg
     8461 tgatgtacgg gaacaatgaa atcggaaatt tgatgccaat caaagaaatc ggtgaacttt
     8521 tagcggatca tccagcttat ttccatacag atgcggtaca agcgtacgga aaccaaacga
     8581 tccatccaga agaattagga atcgatttat taagtatctc tgctcataaa atcaatgggc
     8641 caaaaggcgt cggtttctta tataaacgag acggagtaaa tatccctgca ttgcttttag
     8701 gcggggagca ggaagaaaag cggcgtgcag gtacagaaaa tttagcaggg atctggggta
     8761 tggcaaaagc agttgaactg ctgtcacctg aagaacgtga agcacgtaac aacaaatata
     8821 aaggattcca gcaattgatt cttgatacat tagaagcaaa cgaaatccct tatcaagtaa
     8881 atggtgattt aacgaataaa ctccctcatg tgttgaatct tcgcttaatt ggcgaagcaa
     8941 atgatttgct actgatgaag cttgatctta atggggtcgc tatttctact ggttcagctt
     9001 gtacagcagg aaatatcgag ccttcacacg tattagaagc aatgtatgga gaagatacac
     9061 cgatactaaa agaatccatc cgtatcagct ttggtttggg aaataccgaa gacgacatcc
     9121 agtatttcct tgatcaatta gttcgtgctg tcaaaaaata actagtgaag tcattcttat
     9181 atgaacagat gtctgagaga acgccttcat tttttaggga acttaactga aaaacctagt
     9241 aaaaatcagg ataaactttt ataataaaga gaaactaatt ttatcgaggt gaaaatcatg
     9301 gcatttgaaa caacagcaac ggtactgggc gcaccaatta gctatcgttt gcatcctgat
     9361 gcaaaaagat atacattgcg cgataatgga tttacagaaa caaacggagg caattatcaa
     9421 ttgatccgac cattagatgc cacgccgcaa agcaaagaag gattcaaatt aaaaatcact
     9481 gtatcaaaag atattcaaac attgaaaatg tcgatcacta cagcaaatgg attgaaagca
     9541 gtcaatattt tcaaagatcc aaaacagaaa atgagccaag ataaatttta ttttctgatg
     9601 gatgggatga ttagccgtgg gttattcgaa aaagtcgaat gatctgtgga tataaaaaga
     9661 caactagttt tgaaacggcc tattgttaga tgactaactt tgtaaggacg acgcaaatta
     9721 gttgtc
//
LOCUS       DAHHPA010000098         9659 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.100, whole genome shotgun sequence.
ACCESSION   DAHHPA010000098 DAHHPA010000000
VERSION     DAHHPA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.100"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            185..1183
                     /locus_tag="NBH55_002330"
     CDS             185..1183
                     /locus_tag="NBH55_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1510275.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="NBH55_002331"
     CDS             1295..1804
                     /locus_tag="NBH55_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCD1510276.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="NBH55_002332"
     CDS             complement(1834..2493)
                     /locus_tag="NBH55_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCD1510277.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="NBH55_002333"
     CDS             complement(2495..3259)
                     /locus_tag="NBH55_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCD1510278.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="NBH55_002334"
     CDS             complement(3267..3944)
                     /locus_tag="NBH55_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCD1510279.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="NBH55_002335"
     CDS             complement(3981..5366)
                     /locus_tag="NBH55_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD1510280.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="NBH55_002336"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="NBH55_002336"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="NBH55_002337"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="NBH55_002337"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCD1510281.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="NBH55_002338"
     CDS             6305..7162
                     /locus_tag="NBH55_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510282.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="NBH55_002339"
     CDS             complement(7243..7578)
                     /locus_tag="NBH55_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510283.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="NBH55_002340"
     CDS             complement(7645..9015)
                     /locus_tag="NBH55_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCD1510284.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="NBH55_002341"
     CDS             complement(9093..9629)
                     /locus_tag="NBH55_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1510285.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2826 a         1997 c         1619 g         3217 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccat
//
LOCUS       DAHHPA010000099         9573 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.101, whole genome shotgun sequence.
ACCESSION   DAHHPA010000099 DAHHPA010000000
VERSION     DAHHPA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.101"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            924..1847
                     /locus_tag="NBH55_002342"
     CDS             924..1847
                     /locus_tag="NBH55_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCD1510286.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1863..2402
                     /locus_tag="NBH55_002343"
     CDS             1863..2402
                     /locus_tag="NBH55_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCD1510287.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3083..3256
                     /locus_tag="NBH55_002344"
     CDS             3083..3256
                     /locus_tag="NBH55_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510288.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            3661..4095
                     /locus_tag="NBH55_002345"
     CDS             3661..4095
                     /locus_tag="NBH55_002345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510289.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            4565..4945
                     /locus_tag="NBH55_002346"
     CDS             4565..4945
                     /locus_tag="NBH55_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCD1510290.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            4927..5841
                     /locus_tag="NBH55_002347"
     CDS             4927..5841
                     /locus_tag="NBH55_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCD1510291.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            6086..6775
                     /locus_tag="NBH55_002348"
     CDS             6086..6775
                     /locus_tag="NBH55_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510292.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(6896..6982)
                     /locus_tag="NBH55_002349"
     CDS             complement(6896..6982)
                     /locus_tag="NBH55_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD1510293.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            7619..8563
                     /locus_tag="NBH55_002350"
     CDS             7619..8563
                     /locus_tag="NBH55_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCD1510294.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            8584..9117
                     /locus_tag="NBH55_002351"
     CDS             8584..9117
                     /locus_tag="NBH55_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCD1510295.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            9132..9302
                     /locus_tag="NBH55_002352"
     CDS             9132..9302
                     /locus_tag="NBH55_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510296.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
BASE COUNT         3609 a         1593 c         1700 g         2671 t
ORIGIN      
        1 cccttttagt gagttttgta gaaagtacct ttaaaggtac tttcacatta cgaacgatac
       61 gtccgtagga cgtactgaaa gggtgggact tatgagcaaa ttaatgcaaa atttggctag
      121 caaaaaagct aaaaaatata tgctaataat cgagcgaaat aaacactttt tcaagtgagc
      181 gaatccgcca tgacaaagct gaagcagttt atttcagctt tgtcatggcg tgtgccgact
      241 tttttaaaag tctaggcaca cgagcaaata tctttatcaa gctctaattt gcgttttaag
      301 acgtttgaaa cgcctaagta tataaattat ataagaatag gtttaagagt gcttaaatcg
      361 aattttagag ggcatttaga caattttagg gttgttaggg cttgccctga ccgcctgtaa
      421 ggcgttttaa gaacaagata agggattttt atactaggac tgtcttaaaa tgtcttaaac
      481 gaacgctcag agcttctcaa agggttcctt atgaacggct tttagcaaag gagccggagg
      541 cgactgcgca gtataatgtg agagcagagc gatcacacct ttttagcgaa ccgtagggag
      601 caaggtttgg aattggcagt tttgataaat tcgcgaacct gtttagagaa aaaaggagtg
      661 attttttagg cgggaaaatt agcctcccct ctttttatat ctctttttat atctcttttt
      721 atatctcttt tatgtcggct gtgtccttac tctcccaagc aattcagagt tttttcaagt
      781 aggttttcta cgcttttcaa gtaggttttc tacgcttttc aagtaggttt tctacgcttt
      841 tcaagtaggt tttccgacat tattgagtat ttttataaag tatgttactt ttatttcata
      901 ctttataaaa gtaggagaag cttatggcaa atgaaattgt aaaatatgaa aatagattaa
      961 atcatattcc tctgagaaaa tttaatagtc gtgaaatgaa cttatttttt tcaattgctt
     1021 cacgtgtacg tgaaaaaggt gtagatgaga ttattttttc atttcacgat ttgaaaaaac
     1081 taagcggtta tacagaacgt ggggaacaat ttataaaaga tcttagtagc acgtatgaca
     1141 aattattaag tattaatgct tggaatgatg atggaaatac tttaactaaa tttgttgctt
     1201 tcacaaaata tagcattata cgagataaac aacttgttat aatttcagtt aatccagaat
     1261 ttaaaggact ttttaatcaa ttaaagaact ggacaagatt taatctagag caatttacta
     1321 atttggatag cacatattca aaaactatgt ttcgattatt aaaacaatat cgaactcaag
     1381 gctggactga attttccaaa gaaaaatttt ttgatttact agatattcct aaaagttatt
     1441 ggaatagtcc gtcaaatatt gacaaattta tcttaaggcc aattaaagaa gaactcactc
     1501 ccttatttaa aggattaact attcgtaaaa aatatggtaa aggtcgaggt aaacctgtta
     1561 tcggttatcg ttttacttgg aaagctgaaa ttaaccatgc tgacgatttt tccaaaggga
     1621 aacaagaaga tttacgtata aaattattta atattgatca caatggagaa ttaacacgag
     1681 aagaaaaatg gcgcgctaaa gatcgaatac taaatcttcc cttaggcact catgaagctg
     1741 attttaaaca acaacaacaa actgaagaaa aacaggcaga aaaacgagaa ctttcaaaca
     1801 aactcaaaca agatttacta ggaaatttac aaaacttatt cgattaagct taaggagtat
     1861 ttatggcaga taaaacaatc aaagaacttg cggaagaatt aggagttagt aaaacagcaa
     1921 ttaacaaaaa agtttctgac aaaaatcgaa aactttggtt tgcaaaaaac ggaaacaagt
     1981 ttgtgattaa cgaaaccggt caaaaagcta taaaatcaat gtttttagtc gataacgcaa
     2041 acttaaatcg aaaactagtt ggcgaaaaat caaaaaccaa aaaccataaa aacaccaacc
     2101 aagtttccga attaaaactc ctttctttcc tagaaaaaca gttaaatcga aaagatatac
     2161 agctacaaga aaaagataat caaataaaac aaatgcaaaa attactcgac cagcaacaac
     2221 aactcactct acaagcgaac caacaaattg agaacctaca agaacagctc catttaactt
     2281 atacagaaga aactccaaca accgaatcaa ctgccttttc tgaaaaaaaa gcagacaata
     2341 cacaaccact tattgaaaaa aagtggtggc aaatttggaa atctaataga accaatctct
     2401 aaaattttct agaagttagt tcggatagta gaggaaaaag ttgtactacc tgatggggag
     2461 atcactcaaa tttttatgag aaagaaaaaa tagtttacaa ctgtaaacaa tcaatgttta
     2521 tagttgtaaa ctatcaaact atatggtgac ttattgctta aaataggttg aaatctcact
     2581 caatttttgg tggaaaaaca aaataaattc taatcgattt tgttgaaaaa gtcataaaaa
     2641 aacaaatgtg attttaacaa aatttcaaaa aaatcgtttg aaatttcgtt tatttttttt
     2701 gagaattttg atcatcgtag ggtcattgta gaatcattat gagatcatcg tgggatcatc
     2761 gaataaagta tgggaaaggc ttgcggtgct tggtattaag ggcattctcc gcctcccagg
     2821 ttcttgacca ttttctctta taagtgggat ttttaccacg ttgagagaaa atggtcaaga
     2881 acctgggagg cggagaaaac ataccaagca ccacaagcaa aaaaagttag agtaacttcc
     2941 aaaaaagaaa aattttttag aacaactttt gaaaatttaa ttttgattta tatccgttca
     3001 atttaaacat ttgataaact gaaggctagt atttaaaatg tgtatacaag tggtagcgtt
     3061 aaaaaaagtg gaggtataac ctatgttaaa aaaaactgtg ttggtactgg gttctatttc
     3121 tctaggaata ttaggtttta gccccatgct attatccgta attggaaaaa acaattccaa
     3181 cagagaactt aaattgaatt caaattatta caacagtcct aacgaagaat tgtttgtaaa
     3241 agaaacggat aaataataaa aaagactgct cttccgagca gccagttaaa aaataatgag
     3301 tgtttattct tgaaaaagca acgcgcttat aaagcgctac caaaaattta tcatatttta
     3361 tgaaaaataa caatccagta aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag
     3421 caactgcaga aaatcaagcc ttaaaaagag ctttttacct agaccaatta aaaatcacaa
     3481 agtgagcccc tgtatcggtt gatacaaggg ctacttggca tactcgacat tatgtctact
     3541 aacctttaaa ataacacgtt ttataaaata aaccctcttg acaatattta attttctgat
     3601 tattctaaat atatacagaa agcgctttcc taggatttta tatctaagga ggaagtaaca
     3661 atgaaaaata aaattattaa aattattact tgtctatttt tgttcggtat gattgttgga
     3721 gcaagtactc cgttaaatct gtttcctttt agtgctagag gtataaaagt ggaagctgca
     3781 agttcacgat ataatcataa tcatcgtgga tttacttgct ataaaatgaa ctattatgtt
     3841 actaataaaa tgtgtagaga tttgaaaaaa aattataata agctaaagac tcctgctcaa
     3901 attgcatctt ttattcctat tggtggagtt ggaacatggg ttattacaaa tgcttttagt
     3961 agtgctgttg atgcaatgaa tgtctttgta agagctgcaa atcaaggaaa aggtgtacaa
     4021 ttaacatata atgctcactt tagcaataca acatcatatc aatacaatga ttacgctcgt
     4081 tatgtaataa aataatcaga aagtgaaact atctcatgaa taaaaagatt ttagctgtaa
     4141 ttatttcaat aatagtttta atcactatgt tcttcatatt taggctaatt ttcaatataa
     4201 gcttacaaaa aagtattctt tatctaattc ctatagcatt tatcttagca atttttagaa
     4261 gtatttacgg aaataaaaac tctaaatagc taaaaataaa tcatactaaa ttttacaaaa
     4321 agtggctagc attttctagc cactttttgt attgtggagc gtgggttttg agggggattc
     4381 aagggggagc ttgcgcccct ttgacaagcg gttatttgca ccaaatacgg aggtatttgg
     4441 ctagcaaata tagagcttgc caagcccttt agtaagcttt agtgagcttt acttattgta
     4501 cctttttagg tacaatggta ttgcgaacga tacgtccgta ggacgtactg aaagggtggg
     4561 acttatgagc gaacaaaatc aatatttggc tagcaatcat tctaaaaaat atacctatcg
     4621 atccgagcca aaacaaatca gttttcgagt gagcgaatcc gaatttgcaa agctgaagca
     4681 gtcagctgaa gctttgcaaa tgagtgtgcc ggctttcgtg aaagccaagg cacaaaacgc
     4741 gcgtctggtg acaccgaaag tggcgccaga cattgctcag gcaatcgcgc gcgatttagc
     4801 caaagctggc gggaatatca atcaaattgc caagtggtgc aacacacacc aacacgacgt
     4861 tgatcctggc gacgcccaac gcttatccga aaacctaaaa atcatgcaaa aggagctaca
     4921 aaagatatgg caacagttaa agtaagccga accacttctt gtagccgagc gatcaattac
     4981 gccgaaccac gagcaaccgt caaaacaggg ataaactgtg acattgatta tgccaaaagc
     5041 gaaatgaaac aaattcgcat gctgtatgga aaagacgatc gtgtacaagc acacttattg
     5101 atccagtctt ttcgaccagg agaaattacc gctgaaaaag ccaatcaact aggggcagag
     5161 tatgcgaaaa aaatagcccc tgaccaccaa atcgcgattt acacacatac agacaaagac
     5221 catattcata accacattgt cattaactcc gtcaatctgg aaactgggaa caaatttcaa
     5281 gcccatggtc aagcattttt aaacaaatgt tatgacctaa atgacgagat ttgtcttgaa
     5341 catgacttaa caatcactga acgagggaag aaacccgaaa aacgaaccat gtctgaaatc
     5401 aaattaaaag aaaaaaatga acctgtttgg aaagacgaaa ttcggtttgc gattgatcaa
     5461 acaatgaaaa acaaaaaaac tcgaacgtat aaccaatttt gtgattctct taaaacattc
     5521 ggcattcatt gcttgaatcg tgggaaaaat tttacttacg aattgatcca caaaaagaaa
     5581 aaagtacgat ccaacaaact aggaaaggat tacgaaaagg agacgatttt acatgagctg
     5641 gacagacgag aacgagccta caatcaacaa cgaaccaacg agctacgaag agctcgagct
     5701 agcattcgcg aaactgcaaa agaagtacga agagaacaac gaactcttac gactgaacaa
     5761 ccggaaatta gaccgactcg aagaaaccat gaccaacacg ttgaaagagg agcagactac
     5821 caacaagaat tatcgttcta aaacgttaca agacatagaa aatgaaataa agaaacaacg
     5881 tgcttttctt gcaccttata ccaaagaaag aatacaaact acaattgatt cacaagtcaa
     5941 aaaattccac catgcgctat taaaccagcg aaataaagaa cagaaaaaag taattatgca
     6001 gcgatcaatg gatattcttg cccccttatg ttgcttaatc atgacactgt tagccttttg
     6061 gctggtatgg ggagaataaa tagaaatggc attctattta ccctatctac tcatttttgc
     6121 cagtatttta ggaattattt ggcttagcta taaggcttat caaacaagat attcattaaa
     6181 aggctctaaa attcgattta agaggttttt attgccaggg gttatattta cattatcaat
     6241 cgttttaagt ggtttattcg gcgttttaga gggaaataaa cgtatttcaa gaagtatttt
     6301 attaggggat atcactcaaa agtatgaagc agcatgtgat aaaaagaaaa aggagcaagt
     6361 gttaacacaa aaaatggaag agtttaccac ttgttatgag gaaatgaacg atatttttgt
     6421 gaatcaagaa aaacgtttga cggataaaaa catggaaaaa ctcactcgtc tttatcaaaa
     6481 cttgccagaa aaacagcaaa aagaggttca agacaactat gagcgaacca aaaaagacgt
     6541 gcaatatttt aaagatacgc agacagaaga agcttgttat aatttattca gtgatacaat
     6601 ccctttttca actagtgaac aggaacgaaa agaacggcaa caatcagtta cctacgaacg
     6661 ttataaagca ctgcttcagc aagcaacgaa catacaaaat ccaacgaaaa aagaaacggc
     6721 attaaattat ctaaaaagtg tcaaggaatg gttagatcag caacagcaaa attgaatttg
     6781 atcggcaatc atgttaaact aaacatgcaa atgatcaaac attagataca cgaaagcccc
     6841 agagaggtcg gattctctgg ggcttttttt caggttgatg tcgcctttat tgattttact
     6901 tcttgtttcg gttattcaac caataactga ataaagagac acaaacttct ataagaaggg
     6961 ggcaaatgaa caattcaaac attagataca cctccttcca gtcgcaagac tgtcggatag
     7021 gcgacaagtt attatatctt aaaaaattaa acttgtgtgg tgtttttaag tatcgcttat
     7081 tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaagct agataaggat
     7141 ttttatccaa gactgtctta aaacggctta gataagcatt cagaaccgct caaaggcctc
     7201 tttaccaaca tgtttttagc aaaggagcct ttaggcgact gcgcagtaca atgtgagagc
     7261 gtagcgatca cacctttttg gcgaccgtag gaagcaaggt tttggattgg cagttttgaa
     7321 aaatgcgcga acctgtttaa agaaaaaaga agtggttttt aggcgggaaa attagccccc
     7381 ctctttttat atctcttttt aaacctcttt ttaaacctct ttaccgtaag ctgtgtcctt
     7441 actctaccaa tggatacaga atgttatgaa ggacattttg tctgtttatg aatgacattt
     7501 tgtctgttta tgaatgacat tttgtctgtt tatgaaggac attttgtctg tttgaacgtc
     7561 aaatatgaat gtcttgacat ttaaaaaatg tcattcataa ttggattgag gtgattatat
     7621 ggctaatgag attgtaaaat accaccatga attaaataca attcctttaa gaaaatttac
     7681 accggttgaa atgaatttgt ttttttccat cgtgtctaga atgcgtgaaa aaggggataa
     7741 gacagttcgt tttacgtttg accaactaaa agatttaagt aactacaaag caactgcaaa
     7801 tgtacgattt gtagaagatt taaaagcaac ttatgaaaaa atcttatctt tacgctttgg
     7861 tcgtagaagt gagtctgggc tttcttacga tatgtttgta atgtttacag aatttaaaat
     7921 caacggggac gtagaaaatc cttacgtaga tatacgaatt tacgaaaaag cactgccttt
     7981 gttaaataat ttagacgagt gggtacgcta ttctttacaa caattcaacg agctacaaag
     8041 tggctatgca aagactatgt ttcgtctgtt aaagcaatac agaaccaaag gtttcgcata
     8101 tttctccaaa gaagactttc ttgaattatt agatattcct aaaagttata agcaacctga
     8161 tatagataaa cgagttataa aacctatacg tcaagaacta actgcaatct tcaaaggact
     8221 aacaatcaaa aagaaatacg gaaaaggacg gggaaaacct gttattggct atcaatttac
     8281 gttcaaacct gagatcaaaa aagcagacga ctttaacaaa catgcaccaa ccaaagaaga
     8341 ccaaaataaa gcattggctt tagctgataa acaaaaagaa tacgaagaac ccgctacaac
     8401 aattaacgaa aaaatcaaac agaagaaacg agaacttgaa ctcctccaac aaatcaaaca
     8461 actagaaaaa gagaaaaaag ctcttgaaaa acaaacagag tccgcacttt cggaaaaaga
     8521 gaaaaaaagt ttattatctg atttagataa tttattccag tagcaaccta ggaggcaacc
     8581 agtatgcaac cacaagaaac ctttacaata aaggaattgt cagatctttt taagatgagt
     8641 aggcaagcaa tatcgaaaca tatccaaaaa ttagattctt ccatgatagc taaaaatgag
     8701 cgtggttata aggttgtatt gcgttctgga gtattgcaac ttgctcgcaa cttagacaat
     8761 caaaggttgc tagataagtt gcataaacat gaacaaaaca acgataaaaa tgcttatata
     8821 ccaaacaaaa acgtggttgc aaacaagttg ctagatcagt tgcgtgaaga aaatgaattt
     8881 ttacgagaac aacttgccct caaagagcaa aataattctc atatacaaaa actactcgac
     8941 cagcaacaac aactcaccct gcaagccaac caacaaatcg aaaaacttca ggagcaactg
     9001 cagttgactt atacggaaga gtcttcagag aatagttcaa cgactctttc tgaagcaaca
     9061 gaaacaatgg acgaacaaac aaagaattgg aaaaaatggt ggcagctatg gaaatgaaga
     9121 aggaaatact tatgacaaaa aaacacttgc tattcggttc tatcctacta ggaatattaa
     9181 gctttagccc aatgttatta tcagtggcca acaaaggaaa taaaaaaggg gaactaaaac
     9241 tcaatcctaa ttactacaac agtcaaaatg aggaagtatt tgtagaaaaa cttgataaat
     9301 aagaaaagac tgctcgtacg agcagtcttt ctgaaagaga ttatcctatt attaacctta
     9361 gaaaagaata ataaatgaat atgagatatg taaaaagcat aacaaacaca aacaaaaata
     9421 acaagtattt aaaaaatgac cgatcaatat tcacaaagaa ttagaaagaa acaaccgcag
     9481 aaaaccaagc cttaaaaaga gcttttaacc tagaccaata aaaattcaca aaatcagcgc
     9541 ctggatccct tggtacaggc gttacttggt agt
//
LOCUS       DAHHPA010000100         9323 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.102, whole genome shotgun sequence.
ACCESSION   DAHHPA010000100 DAHHPA010000000
VERSION     DAHHPA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.102"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(383..880)
                     /locus_tag="NBH55_002353"
     CDS             complement(383..880)
                     /locus_tag="NBH55_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1510297.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLFPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(920..1729)
                     /locus_tag="NBH55_002354"
     CDS             complement(920..1729)
                     /locus_tag="NBH55_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1510298.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2063..2920
                     /locus_tag="NBH55_002355"
     CDS             2063..2920
                     /locus_tag="NBH55_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCD1510299.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2913..3815
                     /locus_tag="NBH55_002356"
     CDS             2913..3815
                     /locus_tag="NBH55_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCD1510300.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3848..4198
                     /locus_tag="NBH55_002357"
     CDS             3848..4198
                     /locus_tag="NBH55_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCD1510301.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4247..5170
                     /locus_tag="NBH55_002358"
     CDS             4247..5170
                     /locus_tag="NBH55_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCD1510302.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5248..6141
                     /locus_tag="NBH55_002359"
     CDS             5248..6141
                     /locus_tag="NBH55_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1510303.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6179..6742
                     /gene="amaP"
                     /locus_tag="NBH55_002360"
     CDS             6179..6742
                     /gene="amaP"
                     /locus_tag="NBH55_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCD1510304.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6756..6944
                     /locus_tag="NBH55_002361"
     CDS             6756..6944
                     /locus_tag="NBH55_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCD1510305.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6957..7859
                     /locus_tag="NBH55_002362"
     CDS             6957..7859
                     /locus_tag="NBH55_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1510306.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7891..8133
                     /locus_tag="NBH55_002363"
     CDS             7891..8133
                     /locus_tag="NBH55_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD1510307.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8389..8760)
                     /locus_tag="NBH55_002364"
     CDS             complement(8389..8760)
                     /locus_tag="NBH55_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510308.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            8984..>9323
                     /locus_tag="NBH55_002365"
     CDS             8984..>9323
                     /locus_tag="NBH55_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1510309.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVR"
BASE COUNT         3224 a         1618 c         1801 g         2680 t
ORIGIN      
        1 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt
       61 cttatgtttt tacttggctg ttcttattac tgcagatgca agcttttgaa taccgtttaa
      121 ggaagaggtt gtgaaatcag cgttttgacc tgagtattag cttaacaaac ggaaaaatag
      181 ttcctcatat tttgccgttt gttaagctaa taccgagggt cagctgattg aacaccattt
      241 aagaaagagg tcgtgagcct gccgtttcac tctgtgtcat aaaaaagcca atctactttg
      301 atagaaaaag cacgtaggac taaaacagtt ctgcgtgctt tttttattgt gtgttaaata
      361 tcgtaaatga agatagatat ttttatgatt cgacgataga gacgttgaaa tctttcaaca
      421 gctctaaagc ggattctccg tgttttctac tggagactaa cattggatca acaaaaaagt
      481 ctgcttcagg aaaagcactc tgcaggccta tcagattaga gagtacacac aagtttgttt
      541 caatcccgac tacttcgatc tctttttgtt cttctttgct ttcaaataaa gtttcctgga
      601 tttcagctaa agtttcaggt ggaatcgtaa agtaatactt tttaatcact cgttcatttt
      661 cttgtggaaa aagaagaggg atcaattgca aatcttctcg ttcttcttct ttgtccttca
      721 gttcaactgg tatatcccgt gtgaagagaa tatattcatt attttggcgt gctttagcta
      781 atctttgagc aatacgttct gccaattgat ctgactctgg aatataaaat tcgctgtttg
      841 gatcaagaat atgattttgc atatcaataa taactaacat tttctttcct cctacattca
      901 tatcgtcatt ttattctttt taatagaagt tttttaaaat ataagcaaca ccagcttctt
      961 cattcgtcag tgttacttgg tttgcaattt cttttacttg tcgctgtgca ttccccatcg
     1021 caatactcaa cccgctgtaa ttcaacatat caatgtcgtt ttcttgatcg ccaaaagcca
     1081 tgatgtcact cgattcgatt ccaaggtaat ccgccagaaa aacaagtgaa tgagctttag
     1141 tgatacctgt aggcgtgatt tctaagaaat gcggatcaga gaatactgat tggataccat
     1201 ctgcttgcag atctctttgt agcttctgta gtttttcttc actcttatct gttaatgcta
     1261 atttaagata gctaagagaa ggatcttcca acatatttaa aatttgatca aaagaatggt
     1321 tctgtaaatt gaaatgttca taataagggt catctttgac tagatttgat tcatcgaatg
     1381 tcagctgatc atcttgggtt tctcggctag caatgatgga tattttcgct tcttgagcat
     1441 agaagaaggc ttttctcagt gtatcgctat ctatttttga acgtctgaga actttcgcca
     1501 tttttccatc cttcatttca gcaatcagtg aaccattgga agaaatcaaa tagccaccga
     1561 ttcctaattc ggaaaaaata tctttagcag aaatgctgtt ccttccggta gcgacggtta
     1621 cgattccgcc attttcatca aatttccgaa gtgcttggat attttcttct ggtatctttc
     1681 catttcgatc aagcaaggta ccgtctaaat caatcgctgc tagtttcatt ttatcttcct
     1741 actttctttt tacatatcta ttttcctgtt accttaagat taaatgagaa tttaaaaaaa
     1801 tgaaactaac ttgctttgtc attatgaatt ttttcaaaaa acattcttaa gaaaaaggtg
     1861 gctatatata gtagtttttg gtcaatacct ttgaataaaa agccatgaaa aacgaatagt
     1921 tagcgcttac cgatgcgttt tgtgataaaa aaattttaaa atattcaatg aaaacaaagc
     1981 atatattttg ctttaaaacc aaatgaggaa tattctagat gtagaacagg ttagaaaaag
     2041 attagaaaaa ggagtgattt tgatggagaa gagatacggc gtagttcctg tggtaactgc
     2101 ttcagacgaa aattatgctc cttacttgag cgtgatgatc gcaactgcat tagaaaattg
     2161 caataaaaca agacgcataa aattttatgt gattgacgat ggtctatcag aatatagtaa
     2221 agaaggattg gaagaaacgg taaataaata ttcaagtaat gcaagtatcc aattcctgac
     2281 agtggaaaaa gatatctacg aagacttcct agtcagtgac catatcacaa ctaccgctta
     2341 cttaagaatt tctttaccaa acttattagc taaagaggat tataagaaag tcctttactt
     2401 agattcagat gtgttagtac tagacgatat cgtaaaacta tatgatgagc cgttaaatgg
     2461 aaaaacaatc ggcgctatta tcgatccagg ccaagtgaaa gctttggaaa gattaggaat
     2521 cgattccgat gacttgtact tcaattcagg agtgatggtg attgacattg atcaatggaa
     2581 taaaaaagaa attacagaaa aaactatcca ttacctcagt gaaaatggcg atcgaatcat
     2641 ctatcacgac caagatgctt tgaatgctgt actttatgaa gattgggaac aacttcatcc
     2701 aaaatggaac atgcagactt cgctgatttt tgaacgtcat ccagcaccaa atgaaaaata
     2761 tgaacgtcag tataaagaag gaaatgaaaa accttcgatc gttcacttta caggacatga
     2821 taaaccttgg aatacgttga aagatcatcc gtataccaat ttatacttaa aaaaattagc
     2881 acacagtact ctaacgaaag taggcgagat caatgaataa aaaagaaata gcagtagtcg
     2941 caagttgtaa cacaaaattc gtaccacatt tagctgcatt atttgtctca gtattagata
     3001 attgcaaccc gtctaaattt gttcggttct atgtgattga cgatgatatc gattttgaaa
     3061 gtaaacagct tttacgcttc tcagtgaaaa acgcacgaat gaactcagat gtggaattct
     3121 tgaaaatcaa taaagaattc tttacaaatg tggtaatcag cgatcgtatt ccagaaacag
     3181 cttattatcg tattgcgatt ccggaattgt tccgcggcac agaagttgaa cgtatcttat
     3241 atatggattg cgacatgatc gctttacaag atatctctaa attatggcgt cttgacttcg
     3301 gtgattcaat cgtagcagct gttgaagacg ctgggttcca tcaacgactg gaaaaaatga
     3361 aaatttcagc taaatcaatg cgttatttca actcaggttt gatgctgatc aatgtgaaaa
     3421 aatggttaga tgaaaatatt acgcaaaaag tattagattt catcgaacat aatccagaaa
     3481 aattacgatt ccatgaccaa gatgcattaa atgctatctt acatgaccgt tggttaccac
     3541 ttcatccgcg ttggaatgca caaggctata tcatggcaaa agcaaaaaaa catccaacag
     3601 cagcaggtga acgtgaatac gaagaaactc gcaacaatcc ttacattatc catttttcag
     3661 gacatgtgaa accgtggagc aaggattttg aagggccaac gaagaaatat tacgaaaaat
     3721 atgcagggat gacggctttc cgttgtgtag ctaaattccc taaatatcca aaatatgcaa
     3781 aagttcaacc aaaaacagat gtagttgtta aataatagaa gacaacaact gaagaggggg
     3841 agtaggaatg gaaattacaa tgtctaaaaa tgcagtagag acgttaattg aaaaggttgg
     3901 ggaaaataca aaaatcgcac tcgcgcttat caatgattcc gatccatttc taagggacaa
     3961 aggtgctttt gcaaaaggca gcttttttca aatcattcct tttgtttcgg aatttggaga
     4021 atatgcgact aaaatcgagc atccattatt ggacatctat acatctaaat tagagcaaaa
     4081 ttatttcggt aaacgattga acatggactt caacaaacag cttgacagct tttcattaga
     4141 aaatgaaatc gcagtactcg attataatat caaattaaag aactgcttct tcagctaata
     4201 gtttcgagaa agtccaaaaa ggaggtatac attgagatac tactttgtgg atgaagaaaa
     4261 gaatgtaata gaaacagatg aaaaaaaata taattggctc gtacttgata gtgcaaagcc
     4321 cgaagagatt gagcaagcaa tcgaagaatt cgagcttcct gatgatattt tcgtaggaac
     4381 cgaatatccg gaagaagttt ctcggatgga gcgcctttat gagacgaaac tagagcatcc
     4441 attttctttg gtagtgatta atctagatca gtcagataat cgtatcgaaa aaaaattaac
     4501 gccaatatct tttgtacttt ccgataatct cttgattact tgtatcgata gaaaaaccga
     4561 atttgtggat cggctgctga caaagcatgg aaatcattta aactcgtttg aaaagatcat
     4621 cacgtataca ttattagata tttacaccca ttacgtgaaa gaactaagag agatgaaaaa
     4681 gcgaattgac gctttggata aagcagctcg taaaacaacg gaaaatgaag aactgtataa
     4741 acaggcagat cttgaaagag acatcgtata tatcgatcat accttaagag atcaacggga
     4801 aacaatggat caattatgga attctgaaac tttcatcaaa cgtttagacg atgaacagct
     4861 actctatgat gtccatttga gacagcgaca aacagaaaaa atgatcgaga tctatcgaga
     4921 tttattagag agtattggtg atctattcaa cgggatgatg gacaataatc taaatcatat
     4981 catgaaatat ctagattcag ctgcattaat catttccgtt ccggcgttgt tcgcagggat
     5041 ttggggaatg aatacaggag gattaccggg taaaagttct gctctaggct ttatactcgt
     5101 agtctttggc tctttgatag cagcagtagt tttaggttac catttatacc ggaaagatta
     5161 tacaaattaa agaatctatt taaaacaggt atcaaacaaa gaaatttgtt ttggcaatac
     5221 aatttaaaaa ctaggaggaa tacagcaatg acagaaccaa aattaatcga cccgcgaaac
     5281 ttgtaccata cggagaaatt tcctaaacaa gatcaggata caccagcttt acagcagaat
     5341 atgattcctg ttcctgattg tggagaagaa tcctatgaag gcaataatca actagaaaac
     5401 cgccgtgtac taatcactgg tggagattca gggatcggtc gtgcagcagc gattgcattt
     5461 gctagagaag gggcagatat cgctcttcaa ttcttcccag gagaagaaaa agatgcagag
     5521 gaagtagcaa aatacgtgaa agttgcagga cgtaagattg tcttactacc ttttgatctt
     5581 cgtgacgaac aagcacctaa agaaatcata gataaagccg tgagtgaact tggcgggcta
     5641 gatacgatgg tactaaacgc aggacagcaa atttcttgtc agtctattgc tgagttacca
     5701 cttgagcaag tcaaagacac atttatgata aacatcatcg ctatgtttgc tttagtgaaa
     5761 gaagctgttc cgcatatccc agcaggagga gcaatcgtaa caacaacttc tgttcaagca
     5821 ttcaatccaa gcgaacattt attagattac gcagcaacaa aagcttcaat cgctaacttt
     5881 acgatcggtt tagcaaaaca gcttgcacct aaaggaatcc gtgtaaatgg tgtagcacct
     5941 ggtccaatct ggacaccatt acaactagac gaaggacaac cttctgattc gatccctgaa
     6001 tttggtcagc aaagtttact agaacgtgca ggtcagccag gagaattagc accagtttat
     6061 gttttcttag catcgaataa agcaagttat gttacagccc aaatttacgg agtaactggt
     6121 ggagaagcga tcaacttata agaagttgat cgtttcaccc ctatgaggag gtgcgaaaat
     6181 gaataaaggt gtaaaaacga tcggagtgat cggaacatta cttttgctat ctgtgctttt
     6241 gtttgcgata ctattaagtg caacctatcc attgcctttt cagctagaga agtttcgatt
     6301 cttttccatc actaattcat acgtgcaaca atatgtattt tggcttgccg ttgcatttgc
     6361 tattttgaca attatcacgt tacttgtttt cctgttttat ccaaaaacaa tcggaaactt
     6421 tctactgaaa catggtgatg gggagttgac gttggataaa aaagcaattg aaggaatggt
     6481 tcgttctcac ttgcatgaag atgaattcat ccattcacca aaagtaaatg tcaaagcaac
     6541 gaaaaacaag atcgccgtca atgtaaaagg ggagttgaaa cgaacttcag cactcattgg
     6601 taaaacagga acattgatga aagaaatcga aaatgaagta caacacatat tgggaacaaa
     6661 agagccagtg aaagtatcgg tgaaatacac aggctttgaa gaaccagcta aacgaagcaa
     6721 tgaccgcaaa cgagtagaat agggggggac caaaaatgaa agaactattt tcgacataca
     6781 agctccctat catctttggg gtacttggat tgatattagc cgttttatgg atcaccgtgg
     6841 gctttttcaa gaccttattg atcttgatca tggcagcgat cggcgtcgct atcggttttt
     6901 atctgaaaca aacgaaaatc ttagatcact attttaaatc ttaggaggaa acatccatga
     6961 ataacaataa caaaaataat aacaataaaa tgaacaacca aaaaccagtg tctgcacatc
     7021 cagacccaac gacaccaaac caatcaaatg gtgtgaacga tgaaaagaac aaaacagcac
     7081 cagtatctgc acatccagat ccagtagtcc ctaacgttca acaaggagta gaagagaaga
     7141 aacatgatgc agaaaagcat gcacctcatc atccaggaac tccagaatct aaagttccag
     7201 aacaacatga tgcaatcgat ccttctaaac ctgtggaaga gcaaaaacat ccttctgacc
     7261 atgcgaaagc acaagaaaaa cttagttctg accacacaaa cggacaagga aaacttggtt
     7321 ctgatcatac aaataaccaa ggaaagcatg attctgaaca ggaagattca atgcgcggtg
     7381 agctgaatta tgaagataaa gttgtgcaaa aaatcatcgg aatcgcaatt gaacaagtag
     7441 acggtttatt atctgcaaac ggtggtttct tctctaatgt ggcaggaaaa cttgtgaata
     7501 cagataatgt aacggctggg atcgaaacag aagtcggtaa aaaacaagta gcagttgata
     7561 tggatgtcat tgtggaatat ggcaaagata tcgaaaaaat ctttgaagaa atgcaagaag
     7621 taatcggcaa agaagtaaaa aatatgacac atttagaagt gatcgaagtc aacgctaacg
     7681 tagtggacat caaaacaaaa gaagaatttg aaaaagatca agaaacggtg caagacaaag
     7741 taactgacgc agcgaaaaaa accggtgaat ttgcttcgaa tcaaacagat aaagtaaaat
     7801 cagcagctgg aagcggcgca caaaaagtaa aagaaaatac agaaccacgg gttcagtaat
     7861 aaggaagtaa aacattagga ggaacgaaac atgggattta tttggtcact tatcgtcgga
     7921 ggaattatcg gagcaattgc aggagcaatc acgaataaag gttcttcgat ggggattatt
     7981 gcaaatgtcg tagcaggttt agttggatca gcacttggtc aagcattatt aggttcttgg
     8041 ggaccaagta ttgcaggaat ggcaatcgtg ccatcattga ttggtgccat cattttagta
     8101 ctaatcgtat cattctttgc tcgaaaactg taaaaagact tttaaaaaga ggtcgtgaaa
     8161 aagctgttct tcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttca
     8221 tttggttggg catattatcg aagatgcaag cttttgaaca ctgtttatac ggaataagag
     8281 gctgtgacaa gacttttgtc acagcctctt atttatgcgg gcaaattccc aattagattt
     8341 ttaaaagttt ttccttaaat gtgtctttat tgatacagag aagcttcttt aataaaacaa
     8401 tgcctcccta aattttcttg agataggaaa ggtgaacata caaaataata cgaccacttt
     8461 gaaaacccag atgatccaca aatggatcgt atgatactga tgaagtaaaa agcaatggat
     8521 cagaaactca aagagattga aaaagacaag gaagaaataa tatctctttt ttactcttgt
     8581 ctgttttagc caataatagc tcgtaaagta atctagacaa acaaatagaa gtaaacaaag
     8641 tttcgtaaga gcagcaatcg tatcatgttg ctctgctaga cgattgacga gcaggaaaaa
     8701 tgttggtaat ggaatataga tcacgagtaa tgaaagaaaa aagtaaacaa taaatggcat
     8761 actattcctc ctaattgtgt gagttcgccc tcattttaaa ataacgatat ttgtttgtga
     8821 agctttttcg tgtcgtaaca aatgttatct aatgtatttt taaataaata atcattatta
     8881 tcaaaataat gtcagactgt agacaaagtc cattttatgg atgatgtcta cagtcttttc
     8941 ttttataata gatttatgaa aacgaatgca aggagtgata gctatgatgt ctaaaaacaa
     9001 tacgaatggt agaaatcaat ttgcgatgct tacgattgat gatctagttt ctcaagatca
     9061 tctcgttcga aagattgatg ccgcgcttga ttttgaattt atttatccaa tcgtcgaagc
     9121 cacttactcc gatttaggtc gcccaagtat cgatccagtg attctgatca aattggtgtt
     9181 tattcagtat ttattcggta tccgttccat gcgacaaacc attaaggaag tcgataccaa
     9241 tgtcgcttac cgctggtttt taggctactc cttcgaggag aaaatccccc atttctcaac
     9301 ttttggaaag aattatgttc gcc
//
LOCUS       DAHHPA010000101         9295 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.103, whole genome shotgun sequence.
ACCESSION   DAHHPA010000101 DAHHPA010000000
VERSION     DAHHPA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.103"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(26..694)
                     /locus_tag="NBH55_002366"
     CDS             complement(26..694)
                     /locus_tag="NBH55_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD1510310.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            828..1115
                     /locus_tag="NBH55_002367"
     CDS             828..1115
                     /locus_tag="NBH55_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510311.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1531..3069
                     /locus_tag="NBH55_002368"
     CDS             1531..3069
                     /locus_tag="NBH55_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCD1510312.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3066..4031
                     /locus_tag="NBH55_002369"
     CDS             3066..4031
                     /locus_tag="NBH55_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1510313.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4018..5340
                     /locus_tag="NBH55_002370"
     CDS             4018..5340
                     /locus_tag="NBH55_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1510314.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5403..5507
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5581..6324
                     /locus_tag="NBH55_002371"
     CDS             5581..6324
                     /locus_tag="NBH55_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1510315.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6339..7331
                     /locus_tag="NBH55_002372"
     CDS             6339..7331
                     /locus_tag="NBH55_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1510316.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7343..8080
                     /locus_tag="NBH55_002373"
     CDS             7343..8080
                     /locus_tag="NBH55_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1510317.1"
                     /translation="MSIQISHLNVVIENLKVLEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8070..8792
                     /locus_tag="NBH55_002374"
     CDS             8070..8792
                     /locus_tag="NBH55_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCD1510318.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8876..>9295)
                     /locus_tag="NBH55_002375"
     CDS             complement(8876..>9295)
                     /locus_tag="NBH55_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCD1510319.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3044 a         1603 c         1966 g         2682 t
ORIGIN      
        1 taaataaagt agtgaaaaat aagaatcagt gctctaactg ttcaagagga tcaagtggtt
       61 caaatgtttc tgtagtctgt tttgcttcca attggggcaa aacagatcca atttccagat
      121 acttcacttt attcaatgta taagatagaa tagaacagct aagtaatgta tacagaaaat
      181 gtgtgagatc caaataaagc aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac
      241 ttttgccaat cttaatatga aaatattttt caatggcttg ataaatgata tctgttccgc
      301 ctgtgcttcc gccgaatcga aagacgagac cgcaaccaat gccagaaaga atacctgtca
      361 tcaaactatt gatcagtaga ctatctacag caaaatgtat atgaaggctc tcccataaat
      421 gtaaaaagaa aactagcgca aatgttccga cgatcgtccg gagaaaagcg tgttttccaa
      481 aaagaaacaa tgagataagc aataaaggta aattaaaaag cagtgaagtg acagaaggag
      541 cgaatccaaa aagattatgc aaaagcaaag caataccagt aaccccacca tctgccagct
      601 tagatggaat attgattcct gtcacagcta atccgtagat tcctgcgcct aaaatgattc
      661 cgcacaattc tgtaaataaa tttaattttt tcatttctag cctcttttct tttgaaaatt
      721 ttcagcaact tctttattat actgacttat actcttactt caagaggaaa atgaaaaaaa
      781 attatttttg gctgtttctt tttatttatc aaataaggag tggaaaaatg cgagcagcag
      841 acttattgac aattcttaca aaatcaccca aaaaaacaga gtatcaagga gtttttttgg
      901 ctcaagaaca aaaactggtg cctgtgacaa tggtccagat aaataaagca aatcaattta
      961 tctttttatt tgaacacggc aaagcaccac ttgctatgaa agacatcttt ctttttttga
     1021 tgaagaaccg tgagaaagct gtttattatc agtcaaaaga agaactcatt cctctttatg
     1081 gagtgaaaga gatagaaaat aaagtggtga tataagtaaa aataagaaaa caagacgttt
     1141 atgatgtcaa aacaaccatt ttttacattt ttggcaaaaa agtgtcatga tacagtataa
     1201 tattcgttga acaggccaat aaaggtgttc tcccccacga gatttctact ttattggctg
     1261 tttcttttta ttaaaataaa agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac
     1321 aatttcgtat agatttttat ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc
     1381 gactttgttg cttggattct ttttttgtca gcatatttag aggtattttt ttcttttttt
     1441 tgttcgtgct gtaaggttaa ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta
     1501 tacttaaaga ataacagaaa ggaaggtcgc atgaataatt ttatacaaac attaagtgaa
     1561 agaaaaggcg agttgctgga agctgcattt caacatctgt ccatttctct catttctttg
     1621 ctgattgcag cactgattgc tattccttta gcgatttggg cagccaatca taaaaaaatg
     1681 gcagaagtct tattgcagat cacgggtgtg ctccaaacga ttccttcact tgccttatta
     1741 ggtttactta tcccatttgt agggatcgga actgttccag cattgattgc acttgttatt
     1801 tacgctttgc ttccgatttt ccaaaacacg tatattggtc tagctgaaat tgacccgtct
     1861 attgaagaag cagcggtagc atttggtatg tccagaatga gacgcttgct gaaagtagag
     1921 cttcccatcg cattacctgt cattatctca ggtatccgaa ctgccttagt tttaatcatc
     1981 ggtacggcta ctttggcagc attgatcgga gcaggtggat tagggacatt tattttgtta
     2041 ggtattgacc gaaatacacc tgttttgaca ttgattggtg cgatcagttc tgccttgctt
     2101 gcaattatat tcagcggact gattcgctgg ttgcagcata agaaacctcg atattccatc
     2161 attactttag cagcagtggt gtgtttattt ggcggcgttt ctttatatca gtctgccttg
     2221 tttaaaaatg aaaccatcac gattgctgga aaattaggag cggaacctga aatcctgatc
     2281 caaatgtata aagaattgat tgaagacaac acaaaaacag aagttgaatt aaaaccgaat
     2341 tttggcaaga cgagtttttt gttcaatgca ctggatcaag gtcaaatcga tatctatcct
     2401 gagttcacag gtactgtcct agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct
     2461 gatttgacaa aagaaaaaac atatgacgaa gcagaagaat tattgaataa gcaattcgat
     2521 atgacattat taaaaccaat ggcttaccaa aatacgtatg ccttggcagt caagagatct
     2581 tttgcgaacg aacatcaact taaaaagatt tccgatttgg cagctatctc tgatcagatc
     2641 aaagcgggat tcacactgga atttattgat cgttcagatg gatataaagg aatccaagaa
     2701 gcatatggct tgaatttttc aagtgtacaa agcatggagc caagtttgcg ttaccaagca
     2761 atcaataatg gagatgtcaa tattgtggat gcctattcca cagatagcca gctcaaggag
     2821 tacgatctag tgactctaga agatgatcga gggttattcc cagcttatca aggcgcaccg
     2881 ctgatgaaaa aagattttgc taaagaacat cctgaaatcg ttaaagcact gaataaatta
     2941 agcggaaaaa taacagagga tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag
     3001 aaaaagccga cagaagtagc acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa
     3061 gatcagtgac taaattaatc gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg
     3121 tcatcgatga cctaaatctg gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt
     3181 ccggtagtgg aaaaacaact actttgaaaa tgatcaatgg attatctaaa ccatcagcag
     3241 gagacatcta cttcaaagga aaatcgttaa atgaatataa tctgcagaaa atgcggtgga
     3301 atatgggata tgtgctgcag caaatcgctt tgttcccgac catgacagtc aagcaaaata
     3361 ttgaagtcat ccctgaaatg ttaggttggg aaaagcagaa aagagcagac cgtgtagatg
     3421 aacttcttca aaaagttggc ttatctcctg atatttatcg agatcgtatg cctagagagt
     3481 tgtctggagg agaacagcaa cggatcggga tcatccgagc tattgctgca agcccagatg
     3541 tgattttgat ggacgagcca tttagcgcat tagatccgat ttcaagaaac tcattacaag
     3601 aattggtttt gtccttacac gaagaattgg gcacgaccat cgttttcgtt acgcacaata
     3661 tggaagaagc aatcaagcta ggtgatcgta ttgcatttat gaaggatgga gaaattatcc
     3721 aatgtgacac accagagcag ttattgatga atcctaaaaa cgactacgtt cggcatttct
     3781 ttgatgaacc gaaacagacg aaagagtggc gagtagaaga tttagtcgtc aatggatatt
     3841 tcttaaatga aataccagaa aatgctcgtc aacaagtttg ctttgatacg cctatgaaag
     3901 aagtatattc gcttctttcg gtgtatccaa gcattgtgat agtagaaaag caaagaagta
     3961 ttgggtcgct gacaagtaaa gaaatctttg cttttttaag tcgagaggag gaaggaaatg
     4021 aaaacatatg atgcaatcat catcggttca ggtgtgagcg gcttaagtgc tgcttatgga
     4081 ttgaaagaag caggtaaaac agtactagtg gtcgaagaag atctatgggg cgggacttgt
     4141 cctaaccgtg gatgtgatcc taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg
     4201 gtaaaacaat tgagtggaaa aggttttaac gagatcccaa ctgccaactg ggaagaactt
     4261 caaaaattca aacgaacatt tacggatcca gtcccagaaa gccgaaaaaa acaattagca
     4321 gaagctgaaa tcgatcatct ttcaggaacc gctcgttttt tggatgattc atctattgaa
     4381 gtcaacgaag aagtcttcca tgcggattac ttagttttgg caaccgggca aagacctacg
     4441 atcttaccag tggagggaaa ggaatacttg aaaactagtg cagacttttt atcattgcct
     4501 gtactgccta aagaaatcat ttttatcggt ggtggttata ttgcttttga gctagctacg
     4561 attgccaatg ctgcagggag caaagtaacc attgtccatc ataatcaacg gccattaaaa
     4621 gaatttgagg cgtctttggt agaggaagcc gtccatcaaa tggaagcatc tggcattcag
     4681 tttgcttttg gtgttgaaac ccaaaagatc atttcagaag gtactcgcta tcgtttagtc
     4741 ggaaaagaga cggaattagt agcagatatg attttttgtg cgactgggag acagccgaat
     4801 acagaatctt tagcacttga gcaagcgaat atcgtctttg ataaacacgg aattgcagtc
     4861 aatgattatc tgcaaacgag caatccaaaa atttttgcgt gtggagatat tgtttcaagg
     4921 aaaaccccaa aattgacacc agtcgccact tttgaaggaa attatgtagc taagcggata
     4981 acggatgcaa caagcgaacc tatcaaatat ccgattatac ctacgatcgt ctacgccagt
     5041 cctaaacttg ctgaagtagg agttacaaaa agtcacgcct cttcgtcaga tcaagtggta
     5101 gagatggatc ttaccagttg gttcacctat catcgtgtca atgaaccagt tgctaaggct
     5161 gaattgacct ttgaccagca aaattatttg attggtgctg cagtgattag cgagcaagca
     5221 gacgaattga tcgatgattt gacactcgtc atcaatcaaa aattgactaa aaaagagttg
     5281 gatagctata tcatggggta tccgacattg gcaagtgatc tctcctattt attgaaataa
     5341 tcagcatgat aactaaagcg cttgttgctt tttccttctt gtgctacact ttaaaaaatg
     5401 aatacctgtt tctggggatg tcgtagacga catgagagta agttgtgaac ttagacccat
     5461 tgaacctgtc agttaatact ggtgaaggga gaaacatagc agacgcttga agcgggttac
     5521 tatgtcgtgc ccatttcaag cgttttttgt tattcatatt agaagaaagg aggtaacaaa
     5581 atgaagaaaa atctttatcc agcagtcagt atggcattca ttttaattat ttggcagatt
     5641 gttgtcatag tttggaaaat acctgccttt attttacccg gacctgctgc tgtattttct
     5701 gcattagcgg cagatgcaca agtattactt tcccattctg tcacgacatt aaatgaagca
     5761 gtgcttggtt tggtaatagc tagcgttctt tcttttatta ctgccttggc aatggattat
     5821 tggaagtttt tagaaatgtc tttttatccg ttactagtga tttcacaaat gttgccaatc
     5881 atggtgttag ggccattact taccctatgg tttggtttcg gtatgcttcc aaaagtgatc
     5941 cttgttgtct tgataagcta ttttccgatc gtagtttctt tttcggatac attgaggaaa
     6001 gtatcaaaag agcaaatcat ctttttgaaa acgatggggg cagatacagg gaagatttat
     6061 cgaatatata aaataccagc gggtttatcc ggcttctttt ccggtttaaa agtggctgct
     6121 acttattgtg tcagcggagc agtcgttgga gaatggctaa gcgctcaagc aggcttaggg
     6181 tattacatga tacgcgtaaa aaacagctac cagatcgata aagtttttgc tgcgatcatt
     6241 tgtgtcattc tattgagttt attgctgaat ggcgcttgtt cattattgaa aaaagggtat
     6301 tatcgtatgc tttatatttt ttaagagaag gggatcaaat gaaaaaatta tggcttttat
     6361 taccgctact gttgctttta tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag
     6421 aaataaagga tttgaaaaaa gtgacacttg ttttggatta tgtaccgaat actaatcata
     6481 ctggcatcta cctagcaaaa gaaaaaggct attataaaga ggctggttta aatgtccaaa
     6541 tcatcgaacc aggagataat tcgacaagta ttggtctagt aggtgcggac aaagcacaat
     6601 ttggtgtaag ttatcaagaa gatgtcactt atgctcacgc agatggacag aatataccag
     6661 tcaaagcaat tgctacagtg atcaaacata atacttctgg attcgccacc ctatcagaca
     6721 gtaacatcca ttcaccaaaa gattttgaag gaaaaacata tgcaggttgg caaagtccaa
     6781 gtgaagaagc tgtcttaaaa gctgtgatgg aaaaagacgg cggggacttt tcaaaactga
     6841 caatggtagg aagcaatgga gaagggccag aaagcttagg aaaatcctca gatatccaat
     6901 ggtactttga aggttgggat atgatcaaag caaaagaagc gggtattgaa ctaaactata
     6961 tcccattgaa agaattagat gaacggttag attactatac tccggtaatt attacgaatg
     7021 atcaactgat caaaagtgat ccagaattgg tacagtcttt tatggatgcc acgaaaaaag
     7081 gctatcagga agctattaag gatccaaatg acagtgctaa actgttgcaa aaatatgcaa
     7141 aagaaaatga tcggacgttt ttagaagaat cacaagcatt cttgtcaaaa aattatacgg
     7201 atgatcctga gaattgggga ttgatggaag aaaaagtttg gcgcaattat acagctttta
     7261 tgcaggaaaa cggattgatc aagcaagatg taccttcgca agaactattc actaatcagt
     7321 ttataaaata ggagtgatag atatgtcaat ccaaatcagc cacttgaatg tagtcattga
     7381 aaacctgaaa gtattagaag acatccattt cactatccct aaagatacct ttacatcgat
     7441 tgtcgcgcca agcggagccg gtaagtcaac attgctgaaa actttaacag gagtccagcc
     7501 tatgacttct ggaacgatca aagtagacga tcaaaaagta actggtctcc atacagcatt
     7561 tagttatatg ccgcaagaag atatgttact tccttggctg agcgtctatc aaaacgttac
     7621 gttatatcaa aaaatcaatc atctaacaat caatgaagaa caagtaaggg attatctgga
     7681 tatctttggg ttaaacggct atgaacattt tttgccggaa caattatctg gcgggatgaa
     7741 gcagcgaacg gccttgttac gaacaatcat gaatccttct tcttacttgc ttcttgatga
     7801 accatttgga gcgttagatg ctatgaccag agggcagatg caagactgga tgttgaagct
     7861 gcctaaaaaa gcaaagcgga ctactttact tgtgacgcat gatatcgaag aagcaattta
     7921 tttatcagac aggattcttg tgctttccgc ccgtccggca catgtgattg ctgagattca
     7981 agtgcctgaa aaacgacggt cacgtgaatg gctactgcag caatcagagt tgaagcaaac
     8041 gatttatcag ttattggcag gtgaattaga tgttaagtga tgctcattgt catttagatg
     8101 ggagtcagag tttagcactc cttcagcagg aacacacgat tttgacgatc atcaattgtg
     8161 attcgccaga agaatggaag gaaaacagac agcttgcagc aagtaagact caagccttaa
     8221 gttacggtat ccatccttgg aaagcagata gctatacgtt tgaacaagtt gagccttttt
     8281 tgaaaaaagc aaggatcatc ggtgaaatag ggttagataa tatttggacg aatgtaccga
     8341 tgactacaca aaaaaaagta tttgagcgac agcttgcttt tgcagccata aatgagaaac
     8401 ctgttgtact gcacacaaaa ggatgcgaaa aagagatctt aaattatatc caagcttacc
     8461 ctaatcacta tttgatccat tggtattctt ctttggacta tcaacaagac tatattgatt
     8521 taggctgtta tttcagcatt ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag
     8581 ctaaagcagt cccaatcgac cacttactta ttgaaacgga tggactacat gcagcagagt
     8641 gggctttagg ggggattttt tcagaaaaag attatgtctc actgcttgaa gaacagttaa
     8701 agacaatcgc tgagatcaaa gagctatcag ttgaagaagt taaaaaaaat acttatcaaa
     8761 acttggaacg tttcattaga ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc
     8821 acctcttttt ggctttcttt gtataaaagt aaactgtttg attaataaat gaatcttagt
     8881 accaaccgtt tgcttcccag aatgctttcg ccgcttccca agaaccataa cgtgaagtaa
     8941 catattgttc tgctactctt tcttggtttg cagcagagta gtcaccattt aagtaagaag
     9001 aatctaattg gtaacgtccg atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt
     9061 ctttttgtgc gatccattct tttgcagagc ttgtgcttgc tggagtagca gcagtttctg
     9121 ttacagcagg agcaggttgt gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg
     9181 tttcttgtac aacaggttgt tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt
     9241 cagcagctgc ttgtgcagag ccttctgttg ggattgttaa ttgttgtcct gagta
//
LOCUS       DAHHPA010000102         9205 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.104, whole genome shotgun sequence.
ACCESSION   DAHHPA010000102 DAHHPA010000000
VERSION     DAHHPA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.104"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            298..1485
                     /locus_tag="NBH55_002376"
     CDS             298..1485
                     /locus_tag="NBH55_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HCD1510320.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1466..2770
                     /locus_tag="NBH55_002377"
     CDS             1466..2770
                     /locus_tag="NBH55_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCD1510321.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2796..3809
                     /gene="citC"
                     /locus_tag="NBH55_002378"
     CDS             2796..3809
                     /gene="citC"
                     /locus_tag="NBH55_002378"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCD1510322.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3813..4106
                     /gene="citD"
                     /locus_tag="NBH55_002379"
     CDS             3813..4106
                     /gene="citD"
                     /locus_tag="NBH55_002379"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCD1510323.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            4111..5016
                     /gene="citE"
                     /locus_tag="NBH55_002380"
     CDS             4111..5016
                     /gene="citE"
                     /locus_tag="NBH55_002380"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCD1510324.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            5009..6568
                     /gene="citF"
                     /locus_tag="NBH55_002381"
     CDS             5009..6568
                     /gene="citF"
                     /locus_tag="NBH55_002381"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCD1510325.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6565..7122
                     /gene="citX"
                     /locus_tag="NBH55_002382"
     CDS             6565..7122
                     /gene="citX"
                     /locus_tag="NBH55_002382"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCD1510326.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            7115..7978
                     /gene="citG"
                     /locus_tag="NBH55_002383"
     CDS             7115..7978
                     /gene="citG"
                     /locus_tag="NBH55_002383"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCD1510327.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            8212..8621
                     /gene="tnpA"
                     /locus_tag="NBH55_002384"
                     /pseudo
     CDS             8212..8621
                     /gene="tnpA"
                     /locus_tag="NBH55_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3217 a         1416 c         1853 g         2719 t
ORIGIN      
        1 ctggtgatcc catatttttt agaaatttca ctaatattga tatcctcgag ataatagtct
       61 tcacaaattt ttacaaggag tgattcatag tcagagaatt ccagatgcat agtatagctc
      121 ctaacttttt caaatagata ttttttctca ttataccaaa aaaattgaat ttagtctttt
      181 tttaaatttt taaaaataag catcattttt ttaaaattta aaatgaaagg gtttttttat
      241 tttaaaaaaa gtgttaaagt ggttttgtaa ttaaaaaatg taaaaaggag tgaaggtatg
      301 cttgatgaag ttttagaaat ccataaaaaa aatgtaggtg ttttacggat ggaaacaaca
      361 gaagccgtgc aagatgatca agatttagca aaaatgtaca ccccaggtgt agcagaacta
      421 agtttaatga ttgagaaaaa tcatgctttg gcaagagaat taacaattag tggtaaatta
      481 gtggccgtta ttacagacgg gtcggcggta ttaggtcttg gaaatgtagg aaaccaagct
      541 ggattaccaa tcgtcgaagg gaaagcgctg ctttataaaa atttagcagg tgtcaatgcg
      601 attccattag ccatcgaaca aaaatcagta gacgagattg tacaaacaat tgtcaatttg
      661 caaaatagtt ttgcagggat ccatttagaa gatattgcag caccgaagtg ttttgagatt
      721 gaagaaaaat tacaagaaaa actttctatt cctgtgtatc atgacgatca agaaggaaca
      781 gcgatagttg ttttagctgg attgatcaat gctgctaaga tccaaaggaa aacattaact
      841 gaacttcgag ttttgatcaa cggcatggga gcatctgggg ttgcaacagc tagactatta
      901 atcgctgctg gtatcaaaaa tctaacattg gtggataaac aagggagatt aaagaagcaa
      961 gatagtacat taaataaata tcaaagagag ttgttggatc atatacatga tacaaataat
     1021 aatgcaagcg ttttggaaga tgcaatcgtt ggacaagatg tttttatcgg tctttcaata
     1081 ggaaatgttt taacgcctga aatgattgga aaaatgaatg ataatccgat tatttttgct
     1141 ttagccaatc cagtaccaga gatcacaccc gatttagcaa aagaatctgg tgcaaagcta
     1201 atcgctacag gttcttcgaa atatcctaac caagtaaaca atattttagt atttcccgga
     1261 ttgttcaagg gactattact atcgaaagcg aataaagtcg atctttcatt acaacttgaa
     1321 gtagccaaac aattagctgc taaggtcgca gagcctacgg tagaaaaatt catccctaat
     1381 gtttttgatg aaggagtcgc aaacgtcgtt tcacaggcag ttttggatta tttacaaaaa
     1441 ctaaaagaaa acgaggataa tatacatgct gaaaacatta aatagaatta aaattggttt
     1501 tattccttta tctctttata tcattttggt actcgcgtat cttgcaatga cgataagcaa
     1561 tatcgttcca gaaaatgata tgttagtttc tttgggtatt atgactgttt ttgcttattt
     1621 attagaagaa attggtaaaa ctattcctat tttaaaagat ttaggtggta aagtattagt
     1681 tgttacattt ttgcctgcat ttctagtcta taaacattgg ctacctcaaa gtactgtaac
     1741 tgtggtttct gattttatga ataataacaa tttcttgatg ttctttatca cactactagt
     1801 tgtcggcagt attgcttcaa tgaatcggca aacattggtt aaagcaactt cacgtattat
     1861 cattatttta attttatgcg atgtgattgg ttctttgata ggaacaggtg tgggaatact
     1921 ccttgggcta tctgcttgga atgcgtattt ctttatcgtt gcgccgatta tggctggtgg
     1981 tgttggggaa ggagcgatgc cattatcgat tggttatgca gcaatattat ctggtatggg
     2041 acaaaatgat atctttggga agattctacc gtgtgtgatg ttgggaagtt tagtcgcagt
     2101 tctactgtct ggtatgctca aaaaaattgg agagaaatac ccacaattaa atggtcaggg
     2161 taatttgatc gatggggatg atgaacattt agagaacttg aagggttcag ctaaaccagt
     2221 caatattgaa aaaacattgt tagcaggcgt ggtagcaata acgatttatt ttgttgctgt
     2281 ctatgttgag cacttattaa aatcactcct tggcttttct attccttcac ctgtgatctt
     2341 attagtctct cttatggggg ctaaaatgct tggttttatc cctaaaaata tcgaagaagg
     2401 tggaaattcg ttgtatggat tcactgttcg tgggatcaca cctcccttac tttttggcgt
     2461 aggggttgca aaaacagatt ggttagagct gatcgaagta tttaaaaatc ctgcaacgat
     2521 agtaacaatc attgtgacgg tagtagctat tgtcgctaca gcttttatcg ccgcaaaatt
     2581 tttaaaaatg tacccagttg atactgccat cgcagtttca tgttgtagcg gacaaggcgg
     2641 tacaggtgct ttagctatct tagctgcggg tgatcgcatg gaattaatgc catttgcaca
     2701 agttgctgtt cgactagggg gtgctatgac cgtaacgttc gctatctttt taatgggtct
     2761 gctatcataa gaacataaag aaaggacgtg accatgtgtt tgatcaaaca gatatccaac
     2821 gattattttt aaaaaataac tatatcaaag accaatggga aaatcttttg cttgattcag
     2881 gaatacaaaa gaagcaaatc gaagattttc aacatttaga tcaaacattg ggtatatacc
     2941 ataaagaaaa gttagtagga acagtatctt atcaaaacaa cgtgattaaa tatatcgccg
     3001 tgtctgaaaa atataaagat ggtggacata catttaatgt actagtctct gcaataattc
     3061 aggcaatgag tcaacaagag atttttcatt atattgtttt cacgaaaccg cgctatcaaa
     3121 aaagttttaa ctttttaggt tttgaaacaa tcgtatctac cccaaaaata gctgtattag
     3181 aaatgggaga tagttctata aaacagtacc tagagaggat tcctaaagtg aatggtgaaa
     3241 aaaaagcagc aattgtcatg aatgcgaacc catttacaga agggcattat tatttagtaa
     3301 aacaagctgc caataacaac gattgggtat atgtatttgt actcgaaaaa gaacaaaatc
     3361 ttttttcgac gaaggaccga ctcaaattag ttcaacaagg attacaaaaa ctgaaaaatg
     3421 tcattgttct atcaggtggc gagtatatta ttagccctgc aacgtttccc acctattttt
     3481 tacgagaaga tgatgaaata gcaaaggagc aaatggtcgt agatgctact ttattcaaag
     3541 aaagaattgc cacttattta gatatctctg ctagatatgt gggagaagaa ccactttccc
     3601 caatgactcg ttcatataac gacatactgg aaacgatttt accccctgaa ataaaagttc
     3661 atgtccttgc aaggaagaaa acggaacaac accaagttat ttctgcttct caagttcgta
     3721 aagcgtatct tgctgggcaa ttggaaaaaa taaaatatat ggttccagaa acaacctatc
     3781 aatacttaaa aaataaaagg gagcgttaag taatggaaat aaataaatca gcgattgctg
     3841 gaacattaga gtcaagtgat gtccaaatca tgatttcacc gaatacagaa ggaatagaaa
     3901 tcgatttaga cagtcaagtg attgagcaat ttggaaaaca aatcaaaaaa gttatctatg
     3961 acacactagc agtttatcag attaaaaacg taaaactcaa ggtagtagat aaaggggcat
     4021 tagattgcgt tattgttgca cgattaaaaa cagcgatcca tcgttcctta ggtatccaac
     4081 accaagaaac accgtgggag gtttaattag atgatgaaag aacgtttgag aagaacaatg
     4141 atgtttgttc caggaaataa tccagcgatg atcaaagatg caggtattta tggtgctgac
     4201 tcgattatgt tcgacctgga agatgcagtg tctatggcag aaaaagacag cgcaagaatg
     4261 cttgtggctg aggcattgaa aacagtggat tatggtgaca cagaattaat tgttcgaata
     4321 aatgacctgc actcagaatt tggacgtgca gacatttttg caatggttaa agccggcgcg
     4381 catgtattac gtttacctaa aacagaaacc tctgaagata taacagaagt agaaaaagtg
     4441 attatagaag cagaaaacta tcataatgtt ccgatagggc gaacattgat gatggcagcc
     4501 attgaaggtg cttctggtgt attgaacgca cctgcgatcg ccaaagcatc tgatcgattg
     4561 attggaatcg ccattggggc agaagactat gtgacaaata tgaaaaccaa acgatatcca
     4621 gatggaaacg aattgttttt tgctcgcagt atgatcgttc atgctgcacg tgcagcaggc
     4681 atttccgcat ttgacactgt ttactctgat gtggatgata tggaaggatt tttaaacgaa
     4741 gtacgtttaa tccatcaatt aggttttgat ggaaaatctt gtatcaatcc acggcaaatt
     4801 ccagtagtca atactgttta tcagccgtca atcaaagaaa tcaaacgatc actagacatc
     4861 atctcggcaa tcgaagaagc aaaagaaaaa ggttctggcg taatttcttt aaatggaaaa
     4921 atgatcgata aaccaattgt agaacgtgca caacggacat tgatgttagc aaaagcatca
     4981 cgattgattg atgaggaggg aaatatccat gcataataaa caaagacgta gcattccatc
     5041 aagtgtttta acaaagtata aaaaagaatt ccaaagtgtt tcattatcgc cacaaacgat
     5101 tcaacgttgt gcaccaaaag taacgactgt aactaatcag acaaataaat tgatgcactc
     5161 gatcaaagaa gctatagaaa aatcgggctt aaaagatgga atgacgattt catttcatca
     5221 tcatttgaga gaaggtgatt ttgtttttaa ccaagtgatg gccgtgatcc gtgaaatggg
     5281 catcaaagaa ttgaccttag caccaagttc tttaacgaac actatgaatg agatggttat
     5341 tgaatgcatc aaggacggta cagtaactca tatcacaagc agtgggatgc gcggcagctt
     5401 aggtgaattt atttctcatg gaggaatgga caatcctgtg attttacgtt ctcatggtaa
     5461 tcgtccacgt gcaattgaaa gtggtgaatt gaaaatcgat gttgcatttc taggagtacc
     5521 aacatctgat gattatggaa atgctaatgc gactgttgga aaatccgttt ttggttcatt
     5581 agggtatgca aaagtagatg cacaatatgc agataaagtc attcttctaa ctgatcattt
     5641 agttccttac cctaatacgc ccattagtat tccgcaaaca caagtcgact atgtcgtgga
     5701 agtagaagca attggtgatc cgaataaaat cggtgctggt gctacacggt ttactaaaaa
     5761 cccaaaagaa ttaaaaattg ctgaaatggt caaagatgtc attgttcact caccttattt
     5821 caaagatggc ttttcttttc aaacaggaac gggtggtgca gctttagcag taacgcgtta
     5881 cttagctagt gaaatggaag aaaaacagat caaagcttct tttgcattag gtggcatcac
     5941 taaaccaacg gttgatttat tggaaaaagg tcttgttgaa aaagtgttag atgtccaaga
     6001 ctttgacaaa ggagcagctg aaggaatggc gcggcttgag aaccaacagg agatcgatgc
     6061 ttccttttat gctgatccaa gtaataaagg agcggttgtt aaccaattag atgtcgctat
     6121 tttatctgca ttagaaattg acacaaactt caatgtaaat gttatgactg gttcagatgg
     6181 tgtgctaaga ggtgcgattg gtggacatca agatgcagca acggctaaac ttactatcat
     6241 ctcagcgccg ttagtacgtg gccgaatacc tacagtagta aatgatgtta ctacattgat
     6301 cacaccaggg aaaagtatcg atgtattagt cacagaagta ggggttgcta tcaatcccca
     6361 acgtaaagat ctaatagcta tttttgaaag aattccacaa attcctgttt ttacaataga
     6421 agaattacag caaaaagcag aaaaaatagt aggcatcccg gaacctttgc aattcacaga
     6481 tcggatagtt gcctatgtag aataccgaga tggttcaatt ttagatgtcg ttcgtcaggt
     6541 gaaagaattt gaagaagaga gatcatgatg tttgatactg gagaaattca agaaatcgag
     6601 gatattttga tccataaaga acaacgtgct aacttgcaaa gcaacctgct ttccaagtat
     6661 tcaaatagtt cattgatggt ctttacattg aatattcctg ggccgatcaa aaataatcca
     6721 atgattgagc aggtatttcg tcagggacag cagtcaattc agcaaatgtt tttaaaaaaa
     6781 aaggtggaaa tgacgtacac aaaagaaatc aatttgccaa gtggattgga ctggtacgta
     6841 atagtaacag aagcaccttt gaatctcaaa aaatggttgg tcacgttaga agagcaagac
     6901 gcattaggta gactttttga tttagatgtc ttgttcaaaa aaggagagca gataacaatt
     6961 gtcagtcgcc aagctcttaa tcatggggaa cgtccttgtt ttatttgtgg gaaatcagca
     7021 aaggtatgtg cacgtaatcg tacacatact gtgaaagaaa tgcaaaagaa aatcgaacaa
     7081 atagtagccg attccttaaa aggagcattg aaacatgcat gataatattc aaaaaatagc
     7141 acaagaagca caattagcga tgctttatga agttacatgt atcaataaac caggattggt
     7201 tgatccagta gaccccgggt ctcatcaaga tatggacatt tttacttttc tacaaagtag
     7261 tgtagttttg actccctatt ttgaagaatt tatccaaaca ggattggacc gacaacatca
     7321 accaattgaa gaaaccttta tggcgattcg acagattggt ttagaggctg aaactgcgat
     7381 gttctcagtg acaaatggta taaatacaca taaaggtgct atcttttcat tggggatttt
     7441 tcttgctatt tgtggccggt taactatttg gaaagttcct tgtaaaaaaa gtttctttca
     7501 aaaagaaatt caaaaaatga cgaaaaatct acttaacgat tttgggaaga ttgatcaaac
     7561 aaaaccaaaa gcatggactt ggggagaata tctatttgtt aaccatggat tgacagggat
     7621 acgaggggaa gccaaaagag gttatccttg tgtttttgac cgaggattgc cttattatca
     7681 agcatatcaa gggtcgcaac aagataagat gatagacact ttactttttc tatcaatgga
     7741 agtagaagat accaatttga tcaaacgaag taataataga catgtttttg aagagtatca
     7801 attacttatt cgtccttatt ttgaattagg gggtgtaaaa accatacaag gtaaaaaata
     7861 tttggattac ctaaatcagc aatttaaaaa gaaaaattgg agtattggtg gaagtgcaga
     7921 cttattgatc cttacaatct ttttagataa aatcatggac aaaggatggt tatgttgata
     7981 ctataataca ttattttgta ttagtaagct aataactaaa aaatggttca atacaaatat
     8041 tttataaaaa atggtagtgc ctatcctttc agattgtaga caaagaaaaa tttctttgtc
     8101 tacaattttt ttgttaatga cttcagtcag tttcgtgcgc aaagcgtgcg aaacaagaaa
     8161 accatgcgtt tctacacgtg gaagaactag catatcgaaa ggagtttttt catgtcaaat
     8221 gacgataaaa gtttagccca tactcgttgg aattttaagt atcatattgt atttacacca
     8281 aaatatcgac gaaaagtaat ctataggcaa ttacgaaaag atattggaaa aatacttagg
     8341 aagttgtgca aaataaaaaa cgtggaaatt atagaagcac atgcaacgcc agatcatatt
     8401 cacatgcttg ttcaagtacc atcaaaaata agtatatcca gttttatagt tatctgaaag
     8461 atcgtagtgc tgtgctaatc cacgagcagc acgtaaatca caaatataga tacggaaata
     8521 aatcattttg gtcggaggaa tattatgtga gtacagtcga attgaatcaa aagacaattg
     8581 aaaaatacat ccgtgaacaa gaagctgatg atcgcatttg agatagtata agtaaaagag
     8641 agtagatgtt gcaataacaa aagggtaaaa atggactacc tccagtaaaa taccgaaaaa
     8701 tagtttaact tttggagata agtccattta agggtctata gaatgacaat ttttttaggg
     8761 gattttacta ttattacttc aagacccgct tggattcgca aagtgagtga actcttaaaa
     8821 ctacctgata gtaggttgtt ttaagagtta tctgaaagta aaaattgcat ttatattttt
     8881 gataaatacg taaatttata tatacgtatt tatcaaagtt ttaggccaaa ggaatattgt
     8941 gtaaatacac taggattgaa tgaatagacc agaagaaatt gcgtccaaga acaaaagaag
     9001 catgatagtg ctctatataa attgggcatt caagaatctg aaatttaaag gtagaatttt
     9061 agttagactt ttagatttta aacacaaaaa taaccataca agcaacaaga agcaaagtgc
     9121 tatattataa attgtccgtc cgtttactta cataagatca ttggggctat ttttaaaaga
     9181 gaataataga taataaatga aaaaa
//
LOCUS       DAHHPA010000103         8955 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.105, whole genome shotgun sequence.
ACCESSION   DAHHPA010000103 DAHHPA010000000
VERSION     DAHHPA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.105"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            33..332
                     /locus_tag="NBH55_002385"
     CDS             33..332
                     /locus_tag="NBH55_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510328.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(429..863)
                     /locus_tag="NBH55_002386"
     CDS             complement(429..863)
                     /locus_tag="NBH55_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1510329.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1095..1805)
                     /locus_tag="NBH55_002387"
     CDS             complement(1095..1805)
                     /locus_tag="NBH55_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCD1510330.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1795..2655)
                     /locus_tag="NBH55_002388"
     CDS             complement(1795..2655)
                     /locus_tag="NBH55_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCD1510331.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2660..3298)
                     /locus_tag="NBH55_002389"
     CDS             complement(2660..3298)
                     /locus_tag="NBH55_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCD1510332.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3313..3612)
                     /locus_tag="NBH55_002390"
     CDS             complement(3313..3612)
                     /locus_tag="NBH55_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1510333.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3655..5139)
                     /locus_tag="NBH55_002391"
     CDS             complement(3655..5139)
                     /locus_tag="NBH55_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCD1510334.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5159..5620)
                     /locus_tag="NBH55_002392"
     CDS             complement(5159..5620)
                     /locus_tag="NBH55_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1510335.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5638..6705)
                     /gene="ulaG"
                     /locus_tag="NBH55_002393"
     CDS             complement(5638..6705)
                     /gene="ulaG"
                     /locus_tag="NBH55_002393"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCD1510336.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6923..7693
                     /locus_tag="NBH55_002394"
     CDS             6923..7693
                     /locus_tag="NBH55_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1510337.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7966..8793)
                     /locus_tag="NBH55_002395"
     CDS             complement(7966..8793)
                     /locus_tag="NBH55_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1510338.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2729 a         1874 c         1399 g         2953 t
ORIGIN      
        1 tataatgtat cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg
       61 aactacttaa gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca
      121 aagtgacaga gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg
      181 caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg
      241 ctttaaaaaa aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat
      301 ttgataaaga ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat
      361 actaagtcta tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt
      421 attatatgtt atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa
      481 gaaataccac ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt
      541 ttctcagtat catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc
      601 aaagcttcat tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg
      661 atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc
      721 ttcccttccc cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt
      781 gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg
      841 atcataaggg aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata
      901 atacagttct ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat
      961 tccttaaaat tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga
     1021 taaattttct tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc
     1081 ttaccctagc atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt
     1141 ttatctagta aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg
     1201 gccatcagac aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc
     1261 ccccatgtga acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat
     1321 tttctttcct taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt
     1381 acttcttctt tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga
     1441 gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa
     1501 tgagtatgag taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg
     1561 agatctgaag atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc
     1621 accatttgat ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact
     1681 cctagctcac ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc
     1741 ggcaatgcca gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa
     1801 gacatcatag ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc
     1861 tcttcgaaag tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc
     1921 tagacgtttc aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac
     1981 atcacgaaac tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc
     2041 agtgatataa tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga
     2101 caaattgcct aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc
     2161 taaaaatttt gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat
     2221 gccatattta gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga
     2281 attcattgat ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct
     2341 tagattatgc gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc
     2401 tggatcagca gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg
     2461 tacaccggag ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa
     2521 tcgtgctaac cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc
     2581 ttttacaagg gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat
     2641 cccaatttga gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg
     2701 cggccttcat cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat
     2761 cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa
     2821 agcctagttc aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag
     2881 ataacagcgc atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat
     2941 ccatggcttg ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt
     3001 tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc
     3061 ctgcttttgc aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga
     3121 ctagtttttt atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta
     3181 agcaaagaat tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc
     3241 ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct
     3301 tagttccttc ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat
     3361 ttctttgact gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg
     3421 agtaaacata ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact
     3481 ttttccctct gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat
     3541 tgctttttcc acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac
     3601 taaaattctc atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac
     3661 tctctgcttt tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt
     3721 tttccgatat tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc
     3781 ataacctagg actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat
     3841 cccgatataa ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc
     3901 tacttgtatg attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt
     3961 attggcataa actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac
     4021 gatcgttggt gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc
     4081 aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc
     4141 aattcctgga acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag
     4201 ttcatcgacg aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa
     4261 agaagtcgtc ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat
     4321 caaataatct ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga
     4381 agtagcaacc atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat
     4441 ttttttgtct tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc
     4501 taattttgca aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc
     4561 ttctgtcaat tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc
     4621 taaaatcagt cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag
     4681 taaaatccaa aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga
     4741 acgcagtttc gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag
     4801 taataaaatc atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac
     4861 agcattttgt ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag
     4921 tccaaccaag attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa
     4981 gtaaccgacc gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa
     5041 aatatagcct aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt
     5101 tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt
     5161 aattatattc tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct
     5221 caaccaatcc atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt
     5281 ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt
     5341 cactttcaaa acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac
     5401 catcactttt ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac
     5461 cgtatttttc tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat
     5521 cgatcaatac aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg
     5581 ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta
     5641 taaaaacgac ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata
     5701 aaaattaaac tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca
     5761 gatataaggt ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat
     5821 ctcttttgga tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt
     5881 tgcattcaat gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc
     5941 tcgtgggttt tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt
     6001 ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc
     6061 gaataaatag tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc
     6121 ttctggatct tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt
     6181 gatgtctttg attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat
     6241 tccccatttt tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca
     6301 attttgatga actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac
     6361 gattaaagcg tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa
     6421 attaggctgc atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat
     6481 gccattccca tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc
     6541 ttcatgactt ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg
     6601 atccactact tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc
     6661 tactaaccac ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc
     6721 atatcgttgt tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt
     6781 ttatatgacc gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat
     6841 aataaagaca gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga
     6901 tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa
     6961 agaaattttg gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc
     7021 ccttgatgta tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga
     7081 gatcatacgc aattacggtt attgttctta taattacaaa aaccagactg attttgatca
     7141 atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga
     7201 ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca
     7261 atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga
     7321 taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg
     7381 cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag
     7441 cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga
     7501 aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg
     7561 actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa
     7621 attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt
     7681 agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa
     7741 caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg
     7801 tatccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc
     7861 taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt
     7921 tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt
     7981 ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat
     8041 atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag
     8101 caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca
     8161 taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata
     8221 tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc
     8281 cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc
     8341 agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata
     8401 aaataatgct gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa
     8461 aatttgattt aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt
     8521 ataagattct tggtattttg atacagaatt caatgattga atctcattta tttttttcct
     8581 gattttgtct tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca
     8641 aagcgcactt aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt
     8701 tgcttgttca caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta
     8761 ttcttttagt aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta
     8821 tttctccttt atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga
     8881 ataaaaattt taattttaaa ataatattca aattaaacaa ctggcgtatc tgctccttga
     8941 tttctttttg tgtta
//
LOCUS       DAHHPA010000104         8699 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.106, whole genome shotgun sequence.
ACCESSION   DAHHPA010000104 DAHHPA010000000
VERSION     DAHHPA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.106"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(62..508)
                     /locus_tag="NBH55_002396"
     CDS             complement(62..508)
                     /locus_tag="NBH55_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCD1510339.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(535..711)
                     /locus_tag="NBH55_002397"
     CDS             complement(535..711)
                     /locus_tag="NBH55_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCD1510340.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(873..1301)
                     /locus_tag="NBH55_002398"
     CDS             complement(873..1301)
                     /locus_tag="NBH55_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD1510341.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1393..2253
                     /locus_tag="NBH55_002399"
     CDS             1393..2253
                     /locus_tag="NBH55_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCD1510342.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(2345..2695)
                     /locus_tag="NBH55_002400"
     CDS             complement(2345..2695)
                     /locus_tag="NBH55_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1510343.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2710..4437)
                     /locus_tag="NBH55_002401"
     CDS             complement(2710..4437)
                     /locus_tag="NBH55_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCD1510344.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(4651..5577)
                     /gene="trxB"
                     /locus_tag="NBH55_002402"
     CDS             complement(4651..5577)
                     /gene="trxB"
                     /locus_tag="NBH55_002402"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCD1510345.1"
                     /translation="MYDVIVIGAGPAGMTATLYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5722..7155)
                     /locus_tag="NBH55_002403"
     CDS             complement(5722..7155)
                     /locus_tag="NBH55_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCD1510346.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7155..8558)
                     /locus_tag="NBH55_002404"
     CDS             complement(7155..8558)
                     /locus_tag="NBH55_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCD1510347.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         2369 a         1942 c         1474 g         2914 t
ORIGIN      
        1 ttcttttttt acgaacacaa aaaactgcgg acaatcactg tccgcagttt aaatgataaa
       61 attaagattg taatagttct ttcacaatcg cattcacttg gtttccatct gcttttcctt
      121 tgactttagg cataattgca cccataactt tgccaaattc tttcattgat gaagcaccag
      181 tttgagtgat tgcttcttga acaagttgtc gaatttcttc atcggaaagt tgttccggca
      241 tatatttttc gactattaca atttcacttt ttactttttc agctaaatcg tcacgtcctg
      301 cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac aacaccgtta
      361 gttcttcttc ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt atagatgact
      421 tgatcattcg gatgacttgt aaagattcct tatctttcgc tttcatcgct gctttcatgt
      481 catcgtttaa tctacttagt agtgacacac taccaactcc aattcatcac agaactagaa
      541 ttttttgcgt tttctagctg cttcagattt tttcttgcgt cttacacttg gtttttcgta
      601 gaattcacgt ttacgagatt cttgtaaagt acccgctttt gacacggaac gtttgaagcg
      661 acgaagagca tcgtcaagtg attcgttttt gcgaacgact gtttttgaca tgttaattcc
      721 ctccctccga gcttaaaaag taaccgtaat tgttattgaa atatatctca aaaaacaaga
      781 ctgtcctgta aatattatat ctaatgctgt tttgaccgtc aaggaaatct tgcttttttt
      841 taaaattatc aaatgttatt ttatttaaac ccttattgac aattttcaca tcttccgaaa
      901 atctcgaatc tatgcccttc gatcgtgcat ccacctagct gttcttcgaa ataattcatc
      961 ggacacatat ggatttcttt cgttgttccg cacactgtac agataaaatg atggtgatgg
     1021 ccgatttccc cctgacagca atgaaaacga aacttcattt ctccatttaa atcggtttct
     1081 tccagtaaat cgatctctgc aaaatcatgg agattccgat aaatcgtatc ataacttacc
     1141 cctttgaatt cctgattcat atagtcaaaa acttctttag cagatacgta gcggttacgc
     1201 ttcactaaat aagttaacaa tgcttctctt tttttagtat atttaaaacc gttatttttc
     1261 aatgtttcta atgattgttc gatcaatgtc tgttttttca tgacttattc ctccaaatca
     1321 aaatgattac gaagtacgca ttctagagta tggtataata aacataaaaa aaatgcaagt
     1381 gaggttttat ccatgtcttc cgattctaca gaaatcgtta ctttttttgt catctccgac
     1441 tctgctggag aaactgcgac aaaactagcg caagctacta tggctcagta tcctaccgtt
     1501 gagttcaacc tattccgcag aacgttcgtg acagatgaaa aaacattaaa gcaggcattg
     1561 caagatgcat tagaagaaaa agcacttgtc ctgcatacat taatcaatca agaactgatc
     1621 gagataacac gttctttttg tgaagaacat caattattta gttttgatgt gatgacgcca
     1681 ccgatatcag aaattgaacg tttaaccggt atcaagccaa ttcggcaacc tggtgccctc
     1741 catttattga atgagaacta tttcaaacgg atcaaagcaa tggaatttgc tgtaaaatac
     1801 gatgatggga aagatcctcg tggtttttta gaagcagatg tcgttttgct aggcgtatcc
     1861 cgaacttcca aaacgccttt gagcttgttt ttagctaaca aaaatttaaa agttgccaat
     1921 ttacctttga tcccacaagc acatattccc aaacagcttt gggagatcga tcctaaaaaa
     1981 atcgtgggac taacgaataa cccagatatt ttaaataata tccgtaaaga acggatgcgc
     2041 tcttacggat taaaccctga tactgcttat tccgatatcg aaaaaattag agctgaactc
     2101 gattttgcga atgatcttta tgaaaaacta ggatgtgtag tgatcaatgt ggcatcttta
     2161 tctattgaag aaacggcttc gcttattttg aatgctttag acctagaaga tcatagttat
     2221 tatggaactg aaacaacaga aaaaagcgat taatcatttt tgtctttttc ttaaaaaaca
     2281 ctggagtgtg acaacgaaga aatttcgttg tcacactcca gtgtttatct atttgcacct
     2341 atcgttattg atgttccaca tgtttgagtg tctggttcaa aatatccagt acatgagaat
     2401 catcaagact atacaaaatc gcttttccct cgcgatgaga cttgacgaca tgattttctc
     2461 ttaataaacg tagttgatgc gatacagcag actgctccat ttccactgct tgggtgattg
     2521 ctgtcacatt ttgttccccg tctttcagat atagcagaat tttaagacga gtgggatcac
     2581 tcaacatttt gaataattga ctgactttct ctacttttga tttttgcgtg tctggcaatc
     2641 cattcacctc caacttgaaa aaagactgac ttctaaagtt taccactttt tccatcagtc
     2701 tcaagttttt tattcttctg tcatcttttt gataatcgct tcataagagg ctactttttc
     2761 ggctgcgtct tctgtagagc ttccttttac accaatataa aacttgattt tcggctcagt
     2821 tcctgaaggg cggatagcaa tccagccatt ttcttccaat gtatatttca atacgtcgga
     2881 tggcggtgtt gttagttttt ctacgtttcc ttcacggtct gttctcgtca attctttaaa
     2941 gtcttctgtt tgaataactt ttgctccggc aaattctgtc ggtgcttctt tacggaattt
     3001 ttccatcaaa gcagagattt tcgcagaacc ttcttgtccg ctcattgtca cagaaatcgt
     3061 tttttctgca aaatagccat aggtttcaaa aatcgtttgc aagccgtcat acaacgtttt
     3121 gccttgtttt ttatagtaag ctgctacttc tgctaataaa agcaatgctt ggatcgcgtc
     3181 tttatcgcga acaaaaggtt taactaaata accataactt tcttcgaatc caaacataaa
     3241 agtatgtgaa tggtcttctt cgaattgttg gattttttca gcaatgaatt taaaccctgt
     3301 caacacattg aacatggtag caccgtagtg ttccgcaatt gctgtaggca attcgctgga
     3361 aacgattgat ttcaataccg ctgcgttttc tggtaaagtg cctcttgatt gatgtgcttc
     3421 caaaatatat tgagcaaaca atgccccgat ttggttccca gtcaatactt cataagaacc
     3481 atctggcaaa cgaaccgctg cgcctaaacg gtctgcatca ggatctgttg caatcagcaa
     3541 gtcagctcct tctttttctc caagtcgtat agcatattca aaagctgaat gttcttctgg
     3601 atttggcgat ttgaccgtag tgaaatctgg atctgccaca gcttgttcgg gaactagtac
     3661 gaattgttcg aatccagctt gtttcaacgc acgttcgccc aacattttcc ctgttccatg
     3721 aagtggcgtg tacacaagtt tcaaatcttt ccccattgtt tctaccaatt cacgatcaat
     3781 cgtcactgct ttgatttctt ctagataagc agcgtccact tcttctccga tgatcgtgat
     3841 caacccgcta tgtttagctt cttcgtcaga taatacttcg acttttagcg gattgctgac
     3901 ttcacggaca tatttcgtca atgcatctgc gtctgcagga ggcatttgtc cgccatcttc
     3961 tccgtagact ttatatccat tgtaagccgc tgggttgtga gatgcagtta ccatgattcc
     4021 cgcaaacgtc ttcaaatgac gaactgcaaa agaaagctct ggtgttggac gcagactttc
     4081 gaatacataa gacgggatat tgtgatgtgc cagtgttttg gctgcttcca ttgcaaattc
     4141 tggtgactga tggcgtgagt cataagcaat agcgacacct cggcgttttg cttcttcacc
     4201 ttgtgtatcc atgaaacgtg ctagtccttc tgttgcttgt cttaccgtat agatgttcat
     4261 tcgattgatc ccaggtccta agattccccg catgcctgca gtaccgaatt ccaacggtgc
     4321 ataaaaagca tcttcaagtt tttcaggatc ttcacccaga tcatttaatt gtttttttaa
     4381 gttttcttct aaattttctt catgtaccca ttgttcatac acttgttccc aagacatgaa
     4441 aataacacct ctttctgaaa tatggttctt aataagtata gcattcactt attagatatg
     4501 aaagtatgag gaccaaaaaa cgcttaaatc aagaaaggat tcttttatca aaaggtgttg
     4561 gacgtgttcg cataaatcag attcagtaaa tttcacgcga aaaaaaagag taaaagaaca
     4621 attgaacccg ttcttctact ctttttaaac ttatgcagtt gtttcttcta aagattccaa
     4681 gtatttatac acttgttgtc cagcaatacc gccgtcacca acagctgtag tgatttgtcg
     4741 caaatctttt tcacgaacat ctccgatggc aaaaacgcct ggcatattcg ttttcatttc
     4801 gttgtctgtt ggaatccagc cagcttcgtt cgtcaaacct gcttctctaa aaggtgcagt
     4861 taatggatcc agtccaacat agatgaatac tccgtctgct gctaaatcaa attcattgcc
     4921 ggttttgaca ttacgtgctt tcacacctgt cactaccatt tcgtttccaa cgatttcgtc
     4981 aacgactgta tcccacataa aggaaatttt ttcatttgca aatgcacggt cttggatgat
     5041 cttttgtgca cgtaattcat ctctacggtg gacgatcact acttcagaag caaattgtgt
     5101 caaatagatg gcttcttcta ctgctgagtc cccacccccg atgactacta gacgtttgtt
     5161 acggaaaaac gcaccatcac agactgcaca gtaagaaacg cctcgtccag caaattcttc
     5221 ctcgccaggt acgcctaatt tacggtggat acagcctgtt gcgataatga ctgtttttcc
     5281 ttcgtaactt ttatcttcac agatgacttc tttatggctg ccgtgatcct tgatatcttg
     5341 aacgattcca taagcatttt ctgtcccaaa tcttgagaca ctttcataca tcttataagc
     5401 tagatcgggg cccatgatcg aatcgaatcc aggataattt tcgatttctg ccgtattgtt
     5461 catttggccg ccaggggcac cttgttcgat catcaatacg gataagtttg aacgtgaagc
     5521 atataaagta gcagtcatcc cagcaggacc tgcgccaata acgattacat catacatatg
     5581 tagattctcc tttacttaat caagtaagca tactttacag ccaatgcgaa aaaaagtcta
     5641 ttgttctgct tgcgtttctc gaatagcttt attatactcg aaaaatccgc tgaaaaacaa
     5701 tagactttta taaatcattg atcaatcgtt cgaaagattt tctccatttg aatcaattac
     5761 ttttttatac cagttgaatg attttttctt tgaacgggct aatgtgccat ttccttggtt
     5821 atcacgatcg acataaatga atccgtatct tttcttcatc tcaccggtac cagctgatac
     5881 taaatcaata catccccaag tcgtatagcc taatagatcg actccgtcta gttctactgc
     5941 atccttcatc gcctggatat gcgctgccag gtaatcgatt cggtaatcat cttccacata
     6001 accattctca tctggcgtat cgactgcacc taagccgttt tctacaataa ataatggttt
     6061 ttgataacga tcataaatat cattcatcgt tattctcaag cctaatggat caatttgcca
     6121 cccccattca cttgcttgca gatatggatt tttgacagaa gcaaagatat ttcctgcagt
     6181 ctgttccaag agttcgttgt cggtagtcga aactcgggaa gaataataag aaaaagagat
     6241 aaaatcaaca gtatgttctt tcagcaatgc ggcatcccct ggttccattt cgatctgaat
     6301 gccatttcgt tccatttctt tcaatgcata agctgggtat tctcctcgtg actgaacatc
     6361 aatgaagaaa taattttctc gatctgattt tctagaagcc caaacatctt caggattgca
     6421 agtgtatgga taatatgctc ctgctgccag catacagcct actttgttct ccggatctac
     6481 ttcatgagca atcttcgttg cgatggcact tgcaaccaac tcatgatgtg ccgcttgata
     6541 tttgacttgt tcttcgtttt ctccttcttc aaagtacagt cctgctccca tgaatggtgc
     6601 atgaaggatc atattgatct cattgaatgt cagccagtat ttgacaagtc ctttatatcg
     6661 attgaagatg actcgacaca gattttcata aaaaccaacg agttctctgg aacgccacgc
     6721 accgtattct ttgatcaaat gcattggaca atcaaaatgc gtaatcgtca caagtggctc
     6781 gattccgtgc ttttggcatt ctttaaagac atcctcataa aatttcaagc cagcttcatt
     6841 cggctctttt tcatccccca ttggaaaaat acgactccag gcgatggaca ttcgataagt
     6901 cttgaacccc atttctgcaa acaaagcaat atcttcttta taacgatgat acatatcgat
     6961 tgcaattttt gctggataaa aatgatcctc atcaaattca aacatttttt tctttccggt
     7021 gataacagga aaacggtctt ttccgattgg cacaacatct acgtttgcta gtccgcgtcc
     7081 accttcatca taaccgccct cacattgatt ggctgctgta gcaccgcccc ataaaaaatc
     7141 ttttctgaat cccattatac ttcctccatt atatgtgtat tacctgataa ttctcttgcc
     7201 agttcatctt gaacttcgat tccttcttct tttaatcggc tgattgtttc cttgaattgc
     7261 ggtaaatact gctggtgtgc aataaacaac tcatccatga tccgttttgc tgtctttcct
     7321 gatcgtataa gcggattgat cgtaaacgcc tgtaaagctg tcccataatc accagtcaca
     7381 gctgcttcga tcgtcagtaa ttccatattc ttcattactt gcaaccagcc tctttcagca
     7441 gcaggaagag aaccaaacgc tacatttctc gctccttgtg cgccaacata ggcagtgact
     7501 tctactacac aatctgccgg caggtcagga acagctccat cattctttgt agataccacg
     7561 atctgtgttt ctttatttcc atagattgaa gcaatcgttt cacaggcagc gtctgaataa
     7621 taagcgccac ctcgtttttg caattgttca ggtttatggt ctaattctgg atctttgtat
     7681 aattcaaata gctctgcttc tgtttctttc acttgctgcg ctcttgtacc aatcgtttga
     7741 tattcttcta aagcatgttc cagcatttct tcttcacggt agtaatagcg gtggtagcca
     7801 caagggatca tattcatttg ttttaattgt tctttgaaaa acgggatatc aaagatattt
     7861 ttcggcaatc cattgtcttc atcgtataaa cgatcaatca attctgccgt gcggtcattt
     7921 cctttattat ctgctacttt gtgccaatgg aaatgattta gaccggcaaa cttgtagatc
     7981 agctcttctt ttttcttgcc aagcattgct ggttctgtca tcatcgccat tactggcaca
     8041 ttgcataatc caatcacttt atcccatttg ccatatctga taactgcttc tgtcaccatc
     8101 ccacttgggt tcgcgaaatt gatcagccaa gcatttggac ataaacgctt catgtcttca
     8161 acgattgcca agaccactgg aacggtacga aaagctttga atatcccgcc agcaccattt
     8221 gtttcttgcc caatcatccc ataataacta gggatccgct cgtctttgat tcgtgcatct
     8281 aattgaccga cacgaaactg agtcgtaaca aaatccgcat ctttcaatgc ttcttcccga
     8341 tccagagttg catagatttt gacttcatac ggagaagcat cccacatacg ctgagacatc
     8401 tgactaacga tctcaagctt ttcttttcct tcttctacat ctacgagcca gatttctcgt
     8461 atcggtaatt cctcatatct tttgataaat ccttccatca actcaggggt gtaactgctg
     8521 cctccaccaa tcgtagcgat tttgattcct tttgccatgt tctcactcct ttactttttt
     8581 acactttcat tatctttttt gtaaacaaaa caaacaatag aaatccgttt ctttccagta
     8641 acatttacaa aagttactgt tttctagtgg atttttgtaa atatcttgtc aaaaaaagt
//
LOCUS       DAHHPA010000105         8555 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.107, whole genome shotgun sequence.
ACCESSION   DAHHPA010000105 DAHHPA010000000
VERSION     DAHHPA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.107"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="NBH55_002405"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="NBH55_002405"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCD1510348.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="NBH55_002406"
     CDS             complement(1098..2201)
                     /locus_tag="NBH55_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCD1510349.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="NBH55_002407"
     CDS             complement(2291..3247)
                     /locus_tag="NBH55_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCD1510350.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="NBH55_002408"
     CDS             3528..5462
                     /locus_tag="NBH55_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1510351.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="NBH55_002409"
     CDS             5919..6335
                     /locus_tag="NBH55_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCD1510352.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="NBH55_002410"
     CDS             6349..6915
                     /locus_tag="NBH55_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCD1510353.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="NBH55_002411"
     CDS             6938..7924
                     /locus_tag="NBH55_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD1510354.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="NBH55_002412"
     CDS             complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="NBH55_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCD1510355.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2516 a         1650 c         1739 g         2650 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacgagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgct
//
LOCUS       DAHHPA010000106         8308 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.108, whole genome shotgun sequence.
ACCESSION   DAHHPA010000106 DAHHPA010000000
VERSION     DAHHPA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.108"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            166..1038
                     /locus_tag="NBH55_002413"
     CDS             166..1038
                     /locus_tag="NBH55_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD1510356.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1035..2993
                     /locus_tag="NBH55_002414"
     CDS             1035..2993
                     /locus_tag="NBH55_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1510357.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3325..4176)
                     /locus_tag="NBH55_002415"
     CDS             complement(3325..4176)
                     /locus_tag="NBH55_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1510358.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4299..4766
                     /locus_tag="NBH55_002416"
     CDS             4299..4766
                     /locus_tag="NBH55_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCD1510359.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5029..5511)
                     /locus_tag="NBH55_002417"
     CDS             complement(5029..5511)
                     /locus_tag="NBH55_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCD1510360.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5611..6474)
                     /locus_tag="NBH55_002418"
     CDS             complement(5611..6474)
                     /locus_tag="NBH55_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1510361.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7072..7947
                     /locus_tag="NBH55_002419"
     CDS             7072..7947
                     /locus_tag="NBH55_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510362.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8092..8244)
                     /locus_tag="NBH55_002420"
                     /pseudo
     CDS             complement(8092..8244)
                     /locus_tag="NBH55_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT         2567 a         1679 c         1485 g         2577 t
ORIGIN      
        1 tgattacgtt aacaataatt cgtttcatgt gactagatca tactaggcat gaaaaatctc
       61 tgaggaacat ggtttcccta ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac
      121 tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag
      181 atctttaatc aaaatgcagt attggtagat gacggtggac aggaaaaagt agctattgga
      241 aaagggattg gatttgacag aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg
      301 atatttgtca tggaaccaga aggtcaaatc aagttgcaaa atttacttaa tcagattgat
      361 gaacgctttt tatttgcagc agaaacgatc attgatcatg cggaaactgt tttgatggaa
      421 aagttgaatg aacatgtatt gatcgctttg accgaccaca ttgctttttc tgcggagaac
      481 attaataacg gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt gctttacagt
      541 gatgaattca gtattgctca atgggcagta gagtatctga caaaagagct tgggattcca
      601 tatacttatg acgaagcagg atatatcgca atccatattc acagcggacg aagcggtcgg
      661 aacaataacc atcgcagtat ccgtgaagtg acgattgtat ctgacatcat ccatttagtg
      721 gaatcagaat tggaaacaga tatccattca gaaccatttt cactgaatta ttcaagactg
      781 gtgaatcatt tacgactgct tctacaacga acccatgcgc aacaatatgc tgttttagac
      841 acggaaatcg tagaaatggt caaacgaaaa tacccagaaa gctataagat ttctaaaaaa
      901 atccgtgtgt tgctgaccaa agaataccag ctcgcgatca cgaaagaaga actgggttac
      961 ttggccatcc atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt taacaatcat
     1021 gaggaggaaa aaaattgaaa gcatatctac aacgaatggg acgttccctt caacttccag
     1081 ttgcggtctt acctgctgct gctttactag tagggatcgg aaactggtgg gcatcttata
     1141 gtaacgacat tgtcgctcat tttttacaag ctggcggtaa cgcagtttta ggtcaattac
     1201 ctttgctgtt tgccgttggg ttagcactag ggatgtcagt ggacaaatca ggtgctgctg
     1261 ccttggcagg tttggtcgca tttgaagttc cagtaaatgt tttaaaaact gattcagtag
     1321 ctactttatt gaatatcaaa ccagaatcag ttgatccatc ttttgctcag atcagtaacg
     1381 tatttattgg tattattgcc ggattgattg cggcagctct ttataatcgt ttccacgaag
     1441 caaaattacc gatggcttta tctttcttta gcggcaagcg tctagtgcca atcttggctg
     1501 cctttgtcat gttgctgatc agtgcagtgc tactgctcgt atggcctccg gtttactctg
     1561 ctttagtttc ttttgctaaa ttcattttga gtttaggctg gggtggtgca ggtctttatg
     1621 gcttcttcaa ccgtttactg atccctacag gattgcatca agcaatgaac tccgtgttct
     1681 ggtttgacgt agcaggaatc aatgatattg gtaaattttt ggcttctgaa ggaacaaaag
     1741 gcgtaacagg aatgtatcaa gctggatttt tcccaatcat gatgttcggg cttccagcag
     1801 gagcatatgc tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga
     1861 tgttagcagc agcttttgct tcattcttta ctggtgtaac agaaccactt gaattctcat
     1921 ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat tttcactggg atttctttag
     1981 cagtgagtgc attcttccat tggacagcag gatttgcctt tagtgccgga tttgtcgatt
     2041 acttcctatc tttgaaaaat ccgattgcga atcagccatt gatgttgatc gtccaaggat
     2101 tagtaacagc agtgatttac tactttggct ttaactttgc tatcaaaaaa ttccatctga
     2161 tgactcctgg ccgcgaggaa gcagatcttt cagatgacga tactgcaacg actaattcag
     2221 acaataaata tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga gccgacaatg
     2281 taacttcgat cgataactgt actacacgcc ttcgtttgca agtcaaagat acaggcctga
     2341 tcgatcaaaa taaaatcaaa gccactggcg tacctggtat gaagatcatc gacggaaaaa
     2401 atgcgcaagt catcgtaggt acagaagtac aattcgttgc agatgagatg gcaaaactac
     2461 atggtggtgc tgcaacacga cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg
     2521 ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg attccaatca
     2581 ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg tgacggctat gctgtattac
     2641 cagaaaatgg agaaatcttc tctccaatcg ctggtacgat cacaaacatt ttcccaacga
     2701 aacatgctgt cggaatccaa acagatgctg gtatcgaagt cttgcttcat atgggcatca
     2761 acactgttga cttaaaaggc gaaccattca ctttatatgt aaaagaaggt caaaaagtcg
     2821 caagaggtca attgattgct ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca
     2881 cggatatggt cgttgtattc acaaatggcg acaaagtcca aagcttagaa atcgaacccg
     2941 cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc caacaaagcc tgagccatcg
     3001 tcttccccag acttttttat cgataaacaa acaggagctg agacaaaaat cttgtctcag
     3061 ctcctttttc cgaataaaaa cggcgttctc ctgccaactc cgcggtaata agtccagcca
     3121 aacgtaacat atgaagagct attttttatt tgactcttct tattacttga tgcagaacag
     3181 cttttttaca acctctgata aacggtgttc aaaaaccagt ttatcggtat gaagtcaaat
     3241 ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttcatactca aaactaatcg
     3301 gctttttcac aacctcttta tacattattc ctgatccaat atttctttta atgctgcttc
     3361 aagagaagtt aatggttttc ccaatacttt ttctaaatca tcagaggaaa tagctaactg
     3421 atccttttta atatcatgtt ggatcgctaa aaacatgtca gctgcttctt gagggaatcc
     3481 ggcttctttt agattttcga caaacgctgc atcacttgct tcaataattt ctaacttttt
     3541 acctgtcgca cgctctaatg ccttggctaa ttcttcatag tcagttagat tgccggataa
     3601 ttctaagatt tctggagagt cactttctac tagtactttt gctgcagctt ctgcatattc
     3661 acgtttcaat gcccaacctg tttttccatt tcctgcagca taaacgaatt gaccagtttt
     3721 taaagcctgc ccaatgatag gtaattcatt ctctacatac caattattcc gtaaaaatgt
     3781 atgggaaata cctgaatcct taatcatttt ttcagtgaat tggtgatctg gcgctaatac
     3841 actgatcgag tgatcggcat ctgcaaaact tgtataagcg atataagata ccccagctgc
     3901 ttgtgctgcc ttcactacat tcttatgttc ggcttgacga tttcctggaa cgccagagac
     3961 aaaaagcaat cgatctattc ctttcaacgc ttctttcatt gagtctaaat catcataatc
     4021 accaatacgt atattaaatc cagcttcttt cagtgcttct cctttttctt cactacgtac
     4081 tagtacatag atttcatttt gtggaaccat cttttttaag aattccagcg cataaccacc
     4141 aaaacctcca gttgctcctg ttactaaata atgcatattt gccactccct attcttttta
     4201 tttgttaaga tttacagctg tacatttata aattacacct ttatcataaa gagtgtggta
     4261 agataagtca agcaaaaaaa atgaaaggat gacaactgat gaagtattct ctgcaattta
     4321 gtgatgcaat ccatgtactt gcctatattg aaatgtttca agatacccga tcactttcta
     4381 gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc
     4441 agctcaaaaa atccggtttg atctttacaa aaaccggaaa accgcagcct tcccttgcca
     4501 aaagtccaga tcagatttct ttactagatg tctatcgcag catcgaaggt aatacacagc
     4561 ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat atccaagaag
     4621 ctttagaaga aagctacgct cggctgcaga aaaaggcaga agaagaaatg gctaagatca
     4681 cacttgatag tctgatacat aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca
     4741 caggcctcgt agagagattt ttataattct ctctactgtg cctgcatttt tctgatcgga
     4801 atcgtctaca taaattcttt tattctccgg gggacaaaag ccttgtcatg cccctttttc
     4861 ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt
     4921 agctgaccct cggcattagt tctgaaaaat gcaaaatatg gaaagctatt tcccattttt
     4981 cctctctaat gctcaggtca aagcgctact ttcacgacct ctatattatc atggtttatt
     5041 gactaccggt atccagattt ctcccatata tttcccgttg gtaactccca tttcgactac
     5101 agcatttgga cccccaacat aggcgtaatc ttctacttct cttaaaacat gcccaaaggc
     5161 cgtattcgtc aaatcatttc ttaattcttc aggcgattct gctaaagcga ttacaacaag
     5221 atacgttcct tttgggaatg ggatgattcg ggtagtgttt ggaacatcct gactcgtttg
     5281 aacaccgaca tagtacatca ttttcccgtt atagggttcg ttgactgtaa agaaataatg
     5341 attttcagca gcttgtttca atgcatctat tgttccatta ccgataactt tttgctcaaa
     5401 tgcttgtttc tctgcagcca aaccgacaaa atccgtattg ctactcttca attccacacc
     5461 aatacccgtc accgtaaaat ctggtttttc ttcgatccta tactttgcca tttctcccac
     5521 cctttctatt atattaagtt catcttacat aaaattaaag ggaaatgaca cgattacgct
     5581 cttttttcta gggatatttt tattttttaa ctattttttt gctttcactg caatccaaat
     5641 ctcactgtaa acattactga aatcctctcc ccattttgta aaagagatct cgggagcttc
     5701 gactaactca taatctgaag aaggaagcca ttctgcatag attttccccc aagtatcttg
     5761 taaagtttgg ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt cttctatagc
     5821 aagcatttct aaatcagcgt acggattttc ttttgtcgta gcaaagccaa tcatgtgcat
     5881 caaactgccc ttttcttcca agcgggctcc atcaaatcca taagaaacgt tgattacttg
     5941 atttggatat aaatctccta acttatgcat ctcttccctt tgagtctccg tgatcgtctg
     6001 cgctaacttt tgtatttctt gattgactcc ttcaaatttg acaggaactt ttttcgccac
     6061 acctgccaca taaaactgtt ctttcttttc gatacgaaat tccatcgatt ttcctcctct
     6121 gacatcaata aaaaatgtca agcgtggaaa agatttttgt atgccgtatc tgctgatttc
     6181 tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa aaaccttcga ctgtttcata
     6241 tccatatcta aacgccgttt ctgtcactct tgatccatct aggagatcgc gattagccaa
     6301 agccaatcta cggtttttga tatagtccat tagcgacatc cctgcaataa acgtaaagat
     6361 gcgttttaat tgatattcag agattcctgt attttcggct attattttat aggaaagtcc
     6421 ttctgtcaaa tgttcttcga tgtaatcgat caattggttc agatctttaa gcatatttat
     6481 cttcctcctt gcttgccttt ttatcatatt atcaaagaaa gtcaattact tgatgaatca
     6541 ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc taaagaaaat tctcaagcag
     6601 cgtttcgtaa cggataattg gtattatctg cctatctatg ttttaccttt agaaaaataa
     6661 attttgggga agacacattc taagtaaaca aagcggtaaa aatatctttt attcccttag
     6721 ttaatccgct tactacctac tgtacaaagc aaattgttgt tcaaactgct gatcagaacg
     6781 ctgcttggat ttttctttgc gatacatcat acctctttta tcaattggaa caattattta
     6841 tttctaacaa aatcacttca tttttgtcag ataaataaaa aacaaaaaaa gttccatctt
     6901 taaaacacgc tattctttta ctttttctca cattctctaa gaaaagaaaa ggagaatgaa
     6961 tttttgacaa ttcgagcaaa agtgattatc aaatatgtaa aatcagctac ttttttctta
     7021 aacgtttctt tatactaaat ataaataagt gtttcatgaa aggagaacta gatgaataat
     7081 tggcttgagt attttcctga aaacgttttg gaacgcggtt acagctatca tctgcacggt
     7141 tttgtccgtc atttgaacta tacaagcaag tacttgtccg caacagtttc tggcacggaa
     7201 gattataagg tagtgatcac ttgggatgaa aagaccaaca tgacttgtga ttgtctatat
     7261 gccatagaag gaaaaaaatg caaacacatg gctgctgtct tattcgctta cgaagaacgt
     7321 ccaataaaaa aatccaacta ctcactttcc gaactctcgt cccttgtttc ctccgctagt
     7381 tcctctcttg tacgggagct tttaacagag attcttatag aacatccgca atttatcgaa
     7441 cgcttcaaag ttaagatgcc atttcatgca ataaattatt cagataaatt aacaactatc
     7501 atccataaat acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc
     7561 atggagatgc gaaagttcat ccaagaagca gttgaatcgc tcattcagca aaatgcctat
     7621 cttcccgcat tcgaattgat caatgaagtg attgctactc tcgaaacatt ctattgggag
     7681 cctgaggatg aacgaaccct tcttctgata gaggactgtt attatttatg gaaagaatta
     7741 ttagcagaag cacctcatgc agaaaaaagg caaatgttct cttggttcgt ctgccaagtc
     7801 gatcatactg acgcctctta ttcaaaacgc tactccataa aaatcctgaa agaagacttt
     7861 cgggaaaaag aattttctaa ccaaaagaaa aaaatagata aaaaaactaa agaaacggta
     7921 aagaaagacg atttcaatga aaaatagcaa gtaaacgata acaaccatcc taaatccaca
     7981 agaaatacag cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa gtaaagaaga
     8041 cttaacgcgt acccaatatt agtcatttga ctattaggta cgcgttttgt tttataagtc
     8101 atgacaaatt tccttcgttg ctgggtagat tttccggcat tgttgataga ttttatgata
     8161 cttttctaca ttttgtaaga gaggttccac tgcatctgta tattggataa aattctgaca
     8221 acaactttca ggtgtgtcga accactctaa tctttaaaga taaatattaa aataacgatt
     8281 aataatatta acagtcttta tttttcac
//
LOCUS       DAHHPA010000107         8079 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.111, whole genome shotgun sequence.
ACCESSION   DAHHPA010000107 DAHHPA010000000
VERSION     DAHHPA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.111"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            24..1178
                     /locus_tag="NBH55_002421"
     CDS             24..1178
                     /locus_tag="NBH55_002421"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCD1510363.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1200..1952)
                     /locus_tag="NBH55_002422"
     CDS             complement(1200..1952)
                     /locus_tag="NBH55_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1510364.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2316..4982)
                     /locus_tag="NBH55_002423"
     CDS             complement(2316..4982)
                     /locus_tag="NBH55_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCD1510365.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5323..6774
                     /gene="cls"
                     /locus_tag="NBH55_002424"
     CDS             5323..6774
                     /gene="cls"
                     /locus_tag="NBH55_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD1510366.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7103..7918)
                     /gene="thiD"
                     /locus_tag="NBH55_002425"
     CDS             complement(7103..7918)
                     /gene="thiD"
                     /locus_tag="NBH55_002425"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCD1510367.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2348 a         1686 c         1451 g         2594 t
ORIGIN      
        1 atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc ttttttattc
       61 ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat cctgcaaaat
      121 accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa gacatcatta
      181 cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag ctaatcgaca
      241 tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg gtaattatcc
      301 accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag gcttgctacg
      361 gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca gaacgaaaag
      421 tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt gaagtgacgc
      481 aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag
      541 acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat
      601 ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt
      661 ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc
      721 atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg
      781 aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa
      841 tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga
      901 aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg
      961 aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc
     1021 aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg
     1081 agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct ttttcgatta
     1141 caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt
     1201 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
     1261 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
     1321 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
     1381 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
     1441 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
     1501 ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat
     1561 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
     1621 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
     1681 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
     1741 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg
     1801 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
     1861 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
     1921 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
     1981 ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat
     2041 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     2101 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     2161 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     2221 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     2281 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     2341 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     2401 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     2461 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     2521 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     2581 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     2641 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     2701 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     2761 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     2821 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     2881 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     2941 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     3001 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     3061 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     3121 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     3181 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     3241 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     3301 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     3361 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     3421 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     3481 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     3541 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     3601 atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac
     3661 attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt
     3721 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     3781 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     3841 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     3901 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     3961 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     4021 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     4081 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     4141 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     4201 aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt
     4261 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     4321 gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca
     4381 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     4441 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     4501 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     4561 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     4621 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     4681 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     4741 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     4801 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     4861 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     4921 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     4981 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta
     5041 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
     5101 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
     5161 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
     5221 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
     5281 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
     5341 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
     5401 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
     5461 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
     5521 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
     5581 gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat
     5641 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
     5701 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
     5761 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
     5821 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat
     5881 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca
     5941 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
     6001 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
     6061 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
     6121 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
     6181 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
     6241 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
     6301 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
     6361 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
     6421 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
     6481 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
     6541 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
     6601 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
     6661 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
     6721 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
     6781 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
     6841 tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
     6901 caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc tggacggcag
     6961 gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac
     7021 caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg atgcagggca
     7081 actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga ttgatggggc
     7141 catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag tcttttgcca
     7201 atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata catgcagata
     7261 gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt aaccaaaatg
     7321 attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca tttttagcat
     7381 gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt gctgcttgca
     7441 caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca taaaaattag
     7501 gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa gcagattctt
     7561 ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat ggtccaaaat
     7621 catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct agcatacctg
     7681 ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg tcgataaaat
     7741 ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca catgcggtca
     7801 tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct tgcatccctg
     7861 cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga tcttccatca
     7921 aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt ttctatcata
     7981 tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa tcaaatttat
     8041 ttgaaaaaac atttgcaata aatcagaaaa tagtggtcg
//
LOCUS       DAHHPA010000108         8045 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.113, whole genome shotgun sequence.
ACCESSION   DAHHPA010000108 DAHHPA010000000
VERSION     DAHHPA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.113"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            476..1339
                     /locus_tag="NBH55_002426"
     CDS             476..1339
                     /locus_tag="NBH55_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCD1510368.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="NBH55_002427"
     CDS             complement(1468..3090)
                     /locus_tag="NBH55_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1510369.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="NBH55_002428"
     CDS             3339..3860
                     /locus_tag="NBH55_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCD1510370.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="NBH55_002429"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="NBH55_002429"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCD1510371.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="NBH55_002430"
     CDS             5941..6759
                     /locus_tag="NBH55_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCD1510372.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="NBH55_002431"
     CDS             complement(6862..7059)
                     /locus_tag="NBH55_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510373.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="NBH55_002432"
     CDS             7228..7806
                     /locus_tag="NBH55_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCD1510374.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="NBH55_002433"
     CDS             7901..8038
                     /locus_tag="NBH55_002433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510375.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2402 a         1466 c         1590 g         2587 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctt
//
LOCUS       DAHHPA010000109         7956 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.114, whole genome shotgun sequence.
ACCESSION   DAHHPA010000109 DAHHPA010000000
VERSION     DAHHPA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.114"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..832)
                     /locus_tag="NBH55_002434"
     CDS             complement(<1..832)
                     /locus_tag="NBH55_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1510376.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSLKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     Y"
     gene            complement(1056..2996)
                     /gene="thrS"
                     /locus_tag="NBH55_002435"
     CDS             complement(1056..2996)
                     /gene="thrS"
                     /locus_tag="NBH55_002435"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCD1510377.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3333..3512)
                     /locus_tag="NBH55_002436"
     CDS             complement(3333..3512)
                     /locus_tag="NBH55_002436"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCD1510378.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3559..5382)
                     /gene="cadA"
                     /locus_tag="NBH55_002437"
     CDS             complement(3559..5382)
                     /gene="cadA"
                     /locus_tag="NBH55_002437"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCD1510379.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5660..7939
                     /locus_tag="NBH55_002438"
     CDS             5660..7939
                     /locus_tag="NBH55_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1510380.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2325 a         1700 c         1446 g         2485 t
ORIGIN      
        1 aatattcttg gattgcttct cgtgtttttt tcactttttc gctaagcttt tcgtttttag
       61 ccatatagaa ataaaaaatg atatcgatcg tagcaaattg ggccaaaagt gaattagttg
      121 ccgcacttcg attcgtgatt tcttttggtc tagatgtttg tacgagtaca tcggctatct
      181 tgctaagcgg gttatttcca aattgcgtca acgcaatgat ttcgatattc atgcttttcg
      241 ccagctcggc taatgcgact acatctgcac ttctacctga gtttgaaaca agccaaagca
      301 ctttcttttt ctcattgctg acaagttgcg ggagcaagac gtggatatct ttttcaaaaa
      361 tgattggctt accgacacga gaccattttt gcagaatatc ttctactact aaagaagagg
      421 cgccaacccc atataaaaaa acagtctctg ccgattccaa acactctacg actttttgga
      481 tcgtttcttc ttctagaata tctactgttt cttttatcgc tacttgtgca ttgttaccaa
      541 gtttgttttt gagagagtaa aaggactcat ttgcttctat atcactaaaa ccttcatcga
      601 ttccagtctg tagttctgtt gaaatcgtga ttttgaagtc cgtcagactt tgaaaaccga
      661 tttttttgga aaaacgtaca actgtcggcg cactcaaccc ggaagcttct gctaattcat
      721 tagctgtcat tgtagggacc ttctcaatat taccgataat gtagttggcc aacttttttt
      781 ctgctgaaga ataatcttcc atcatacttt caatacgact aattacgctc ataagttcct
      841 ccaccctttt aatgatagtc tatgtaagta taccatatca gacccttatt cacggtcgta
      901 tactggataa aaaacaaaaa aacaatccgc ccaacagaat atcttatccc atcaatccca
      961 cagattctat tgagcatctg tgtctttgag gaatacctgt tatctattgt tcggattgct
     1021 ttctatctac tactttttta caggaaattt acctattagt tttctctgct gtaatttttg
     1081 acttctgctt ccatagcatc aataaagata ttcaaatctt ctacagctgt ttctttactt
     1141 ccataacgac gtacgttcac tgttgcatct tccaattcct tgtctccaac aactaattgg
     1201 taagggatct tttgtgtttg agaggcacgt atcttatagc ccattttttc attacgatca
     1261 tcaacttcta cacgcaaccc cttcatttgc aaacgttctt tgatttcata agcataatcg
     1321 ccatgtgcat caacagaaac agggatgatt gttgcttgta taggtgccaa ccatgttgga
     1381 aaagcgcctt tgtatacttc tgttaaataa gcgacaaaac gttccattgt agaaacaatt
     1441 ccgcggtgga taactactgg acgatgcgta ttttccccgt cttctccaac atatgtcaaa
     1501 tcaaagcgct ctggcaataa gaagtctaat tgtatagttg ataatgtttc ttctgtacca
     1561 agagctgttt tgacttgaac atctagtttt ggtccgtaaa aggctgcctc gccttctgct
     1621 tcaaagtaat ctagttctaa ttcatccata gcggctttca gcattgcttg ggctttttcc
     1681 cacattgcgt catcatcaaa atatttatct gtattgtttg gatcgcgata gctcaagcgg
     1741 aaacgataat ctgtaatatt gaagtcagca tatactgcca ccatcaattc aagcgtacgt
     1801 ttaaattcat ctttgatttg atcaggacga acaaatgtat gaccatcatt caacgtcatt
     1861 tctcgtacac gttgtaaacc tgacaaagca cctgattttt catagcgatg catcattcct
     1921 aattcagcga tacgaattgg taattcacga taagaatgga tcgtgttttt gtagaccatc
     1981 atgtgatgag ggcagttcat tgggcgaaga acaagcattt cgccatctcc catatccatt
     2041 ggcgggaaca tgtcttcttg ataatgatcc cagtgtccag atgttttgta caactcaaca
     2101 tctcccatta ttggtgtata tacatgttgg taacctagac taacttcttt gtctacgata
     2161 taacgttcga tcgtgcgtcg gattgttgcc ccttttggaa gccagaatgg caaaccagaa
     2221 cctacttctg gggaaaccat gaacaagtca agttcttttc ctagtttacg atggtctctt
     2281 tcttttgctt cttcacggag acggatatat tctttcaaag ctttcttgtc aaaaaaggct
     2341 gtcccgtata cacgttgcat catttgattg tttgagtttc cacgccaata agcacctgcc
     2401 actgataata atttaaatac ttgtatacgg ccagtggatg gaacatgtgg tccacgacac
     2461 aaatcgatga attcgccttg ttgataagca gtaatcactt cttcttctgg caattcacta
     2521 atcagttcta ctttgtacgg atcacttgca aaaatttcta atgcttcctg ttttgaaaga
     2581 acacggcgtt cgatcggcag attttctttg acgattttca tcatttctgc ttcgattgcc
     2641 ggcaaatctt ctgcagttac ttggttttgt ccattatctg tatcgtagta aaaccctgaa
     2701 tcgatagctg gtccaacgcc aaagtggata tttggataga ggcgacgcat ggcttgggcc
     2761 atcaaatgag ctgctgaatg acggactaag ccaagtgcat cttcatggtc tggtgtgatg
     2821 atctcaatac ttgcatcttc ctcgattgga cgagtcaaat caacgagttt tccgttcact
     2881 ttaccagcta aagctttttt tgctaagctt tttgaaatgc tttcagcaat tgccaatgtt
     2941 gttgttccag actcaaattc tttgactgca ccatctggaa aagtaatttt tatcataata
     3001 gtttcctcct tgaaatgttt tgattgatca gatcatcaag ctgaaaaaca aaaaaatcct
     3061 agcatctctt gaaaagagat actaggacga aaagttcgtg gttccaccta atgttcaaac
     3121 gcaaatgcgt tcctcaagcg ttgtaacggt ccgaccgccc ttgcttcaac aagggtttcg
     3181 aaagtggtca tcctttggtt ttctcctgga aaagtttcag cctagctttt cctctcttat
     3241 cgaagactct caaattcagt tggctttctc atcaatcaat tatgtgttta tttaaccact
     3301 aataaataga aaaatcaagt gggaaactaa acttattttt tccattcccc atttacaccg
     3361 tatgttcctt ttttcatctc attgatgatg acatggatgt gttcttttgg tgcctgcgtg
     3421 tttttatgaa cagcttccgt gatatctttc atcatatttt ctaattgttc cggactgcga
     3481 ccttctacta attcgacatg tacgaatggc ataatgctct ctcctctttc tgatcgttct
     3541 tttttcaact cgattctatc atactatgtt tgtcttgggt actgaatcag gaaaaatggc
     3601 tggatctttt tttgctctca aggcactcaa aatagagacc gtatccacga cttcttgcaa
     3661 aacagctccg aataatgcgg ggattagtcc cgtacttgca attaacatca aaaaaataca
     3721 aataaagatg ccaatcagta cagactgttt cgctattctc atcgtatctc ttgaaatcac
     3781 tactgctttt gctacacgca agagatcatc tttcaagatg accgcatctg cactttcact
     3841 agcagctgta gaaccatgag cccccattgc aataccgata tctgcagcag caagtgctgg
     3901 tgcgtcattc acgccgtctc ctaccatcgc aacaggtctt tgatctttag ggatactttc
     3961 taaaatggtc agtttatctt gaggcaagct acgggcatgt acagaatcta attggacttt
     4021 ttctgcaatc gtttgagcaa tggcagatcc atccccggtg atcatcatca aatttttcac
     4081 atcaagtttt cttagctcct tgattgtctt tgctgcttct gggcgaatct gatccgaaaa
     4141 taggatcgtt cctgcaaaag atcgatcaat tgttacatag attgctgtat tggacgtttc
     4201 tatttcttca gctgaaacaa aagaggcacg tccaatcttt acttcatgat tattgactac
     4261 tgcttggatt ccttgaccgg tcacttcttt cagttgacta acaggataca gttggctgtt
     4321 tttcacgttg ttcactaacg aacgagctaa gatatggcta gattcctgtt ctgcactagc
     4381 tgcatacaga agtagctctt ctttttttat tccattgact ggctgaatcg cctctacttc
     4441 caatatccct ttggtaagcg ttcctgtttt atcaaaagca atcgtttttg tacgtgcgag
     4501 cttttcaatc gttgttccag tttttacaac gatcccgttt ttacttgaac ggctcattcc
     4561 tgcaactaaa gcaatgggcg cagctagaat cagtggacaa ggtgatgcca ctaccagtac
     4621 ctcagcaaat cgtaaagggt cttttgagaa ccaccaagaa ataccaccga tcaaataagc
     4681 aatcaacgtg aaagggactg cataacgatc agctaaacgg acaaatcggg caggcttttc
     4741 ttttgattct tcgactagtt taataatcat ttgatactgg ctgtctgcgg cacgtttttc
     4801 agcgatcatt tggatcgatc cgtctccatt taaagaccct gacataatct gatctccagg
     4861 tttcttggtt tccgggcggg actcacctgt caaagaagat tcatcgaccg aagattctcc
     4921 tttggtcact cgaccatctg cgggtacaag ttcgccaggc ttgactagta actggtcatc
     4981 aatatttatt tcatcgatta ctatatcctg caattttcct tctttgaatc gatgggcaat
     5041 tcttggggtg ttatctaaca aagactgcag ttctttaccc gctcgtttat tcgcgtaatc
     5101 ctctagacta tctccgcccg taagcatgat caatatcatc aaactagccc agtattcttt
     5161 gacggctaat gttgcaagga ttgcggtaat cgctaataca tctacgccat atcttcctga
     5221 acgtaacgtc ttgatcattt ccaaaaacat caagaaggcg agaatccctc cagtaaatgc
     5281 gacaatcgca aatgcaaggg ccggctggtg aaaaacaaat tcacttaata aagctatccc
     5341 accaataagc aaagtaagta aaaatttttt gaaatgtgtc attgtttttt ctcctctttt
     5401 atgtgactta gagaggttag taataaattt ccagctgcct ctttcatctt aagcatagtc
     5461 cttgactagc actttttaaa acaataggcc ttaattgaaa acaactatca ctatcagcgt
     5521 ttccaaaatt catcagttct acaaacaaaa atattcagca aggattactt tttcgattca
     5581 tcagttaaaa agtacctttt ttcgaaaaac tatgctatac ttttaattcg ttatttacgg
     5641 cggaaggagc aaacatgcga tgaatgagaa acaactagaa caaacgcacg tggatgaaac
     5701 gatcgaacta atccagatcg aacaaaatat gctggataca aaacaacaag aattgactca
     5761 ggaaatgaat cattcgacaa aagacacagc taatcacaag attcgagctg gttccaatga
     5821 agctttttat gaatctgctg ttgaatatcg ccaacatgag caggaattgc ttttgaaata
     5881 ccatactctt gaagcacagc aaaaacgcct gcaaacgttg tctgtcatga aagatagtcc
     5941 ttattttgcc cgtatcgact ttaaagaaga ccgtgaaaca gaaacattat atctaggtat
     6001 cgcttcttta agagatctgg cagaagaaac aatcgttatt gactggcgcg caccaattgc
     6061 caatctttat tatgaaggtg agattggtcc cgcattttat gaaactgaaa cagatagaat
     6121 agatgtcgat ctgcagttga aacgacaatt taaaatccaa gaaggacgta ttttatctat
     6181 ggtagatacg tcggaagtaa tcaatgatga ctttttgcta gagatattgg atgaatcatc
     6241 aagcaaccat atgaaaaaca tcgtctctac gatacaaaaa gcacaaaatg caatcattcg
     6301 ggacacaaca agtaaagtaa tgctgattga aggaatcgcc ggaagtggaa agacatccgc
     6361 tttgcttcaa cgtattgctt ttctgttgta tcataatcgc aagtggctcg aggctgaaaa
     6421 cgttcttcta ttttcaccca accatttgtt ttctgattac atctcgactg tattgccttc
     6481 attaggtgaa agcggcgtgc ctacacagac attcaaaaat tatttggtgc agctgctgcc
     6541 agagttttct ttactagatg aacagcaaca agaaagtggt tttttgctag gggaaaaaga
     6601 tccgatacaa gtgatgaaaa gcggattgac cttagtcgat cagattgatc gctatatcca
     6661 aggcatcaca aactacggtc ccttgttccg tgatatgaaa ataaatggcc gtacgatcct
     6721 atcaaaggaa tcgattcgcc aatggtataa agaaacaaat gaacaattac ctttacatca
     6781 tagactttct ctattacaaa cgaagctcct gaaaaaatta ggcggtctgc aaaaagacga
     6841 aacacgtcag caatgggtca aagatctggc ggaagaacag ctccaagaac tatacgcaac
     6901 agatccaaat cttgaatata cagaacaaaa ggagcgttca ttacgtaaaa aactggccaa
     6961 ccaaatcgta cgtaaaagat tccgtcgaat ccatcgaggg atcatgaatt atcaatttgt
     7021 caatataacg aaacaatatc tccactttct acaaactgtg ccactttctc ttctaacacg
     7081 acataaaatc agcgagacca ggtggcagga acatttgaca actgttcgta cacacttgcg
     7141 tcaaagagaa ttgcgtcaag aggatgctgt actattcttt ttactgatgc gccgaacata
     7201 tccagtgacg gttacacaca aagctcgcta tatctttatt gatgaaatgc aggatttcgc
     7261 accatcacaa gtggcattgc tgcaagcctt gttcccaaaa gcaaatttga ctttctgtgg
     7321 agatctaaat caaaatgtct ttggaaatga aacgatcgtt ggatcattgg aagacctttt
     7381 cgtggaccgt gaagtcactc gtttccaatt aacgacaagc tatcgctcca caaaagaaat
     7441 cactgatttt gctaatcatt ttattgtgaa agaacatcaa gtagaaacaa cagcaagaaa
     7501 aggagacctt cctttgctca tccaaggaac aacagatgat atcaaattga attggttagc
     7561 agataccatt cgaacgactc agggaaaagc ccggtattgg cgtacagcaa tcatagctaa
     7621 aacacaagaa gaatgcgaag ctctttatgc tcaactgcca gacgagttga aacaacaagt
     7681 ccagctgatt ttaaatgaga gtgactttat gaagcgttcc attattttac ttccagcctt
     7741 tttagcaaaa ggtctggagt ttgaccgagt tttcttgtgg gatgtagatg cagaacattt
     7801 ttcttctcag caagacaaat tgatccttta caccatgtgt acacgtgcga tgcatgaatt
     7861 gacccttttt acctccaata atgaaagtcc tttgttaaac aatatggata tttcatctta
     7921 tgagaaaaaa gtactgtaag taaaaagaca actgct
//
LOCUS       DAHHPA010000110         7902 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.115, whole genome shotgun sequence.
ACCESSION   DAHHPA010000110 DAHHPA010000000
VERSION     DAHHPA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.115"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(134..577)
                     /locus_tag="NBH55_002439"
     CDS             complement(134..577)
                     /locus_tag="NBH55_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510381.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
     gene            complement(665..1030)
                     /locus_tag="NBH55_002440"
     CDS             complement(665..1030)
                     /locus_tag="NBH55_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCD1510382.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            complement(1374..3026)
                     /locus_tag="NBH55_002441"
     CDS             complement(1374..3026)
                     /locus_tag="NBH55_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCD1510383.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            complement(3086..3665)
                     /locus_tag="NBH55_002442"
                     /pseudo
     CDS             complement(3086..3665)
                     /locus_tag="NBH55_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            complement(4897..5613)
                     /locus_tag="NBH55_002443"
     CDS             complement(4897..5613)
                     /locus_tag="NBH55_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1510384.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            complement(5638..6036)
                     /locus_tag="NBH55_002444"
     CDS             complement(5638..6036)
                     /locus_tag="NBH55_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCD1510385.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            complement(6124..7764)
                     /locus_tag="NBH55_002445"
     CDS             complement(6124..7764)
                     /locus_tag="NBH55_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCD1510386.1"
                     /translation="MRLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYLYDQYKDV
                     CSAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCDEPVIINF
                     ADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDDGKKHKLF
                     SGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNWFDIGHAD
                     KYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVEYVRPRIF
                     EYSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKRYSVSGDN
                     IQKSLKDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALLSVSVPRE
                     LFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELAKLFHSVD
                     GKYDFIIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSDLKKIELIE
                     ALLFLSMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
BASE COUNT         2653 a         1676 c         1153 g         2420 t
ORIGIN      
        1 taatcattta gttttatctc ctttttacca accatctacc actaggttac gaactaccta
       61 agtgcccacc atccgccgcg tggaggctgt taatcaggat ggcaaccgcc cggtagaggc
      121 gacaattatg ctatcatttt aatttaagtc tatataacat aatactataa taatatgaca
      181 acaccaacgt tataggaatg gaaacaaatg ccgccaaaac caagttaggg ataatttgat
      241 gaatcgtact aaatacaaga atatggagta gatatatatc atatgagtat cttgaaatcc
      301 taggcattat ctttctaaat ggaataactt gatacattaa aattgcaatt gcaccgtaac
      361 cgaagaatgc aaaaatatca ttaatagact ccatcagtcc accaataatt ttcgatatac
      421 cagcaaaacc aattccaaca atggacgcca taatcacgaa tatattatta aatcttacta
      481 tttttctttt cactaaatac tctgcaacca aacatgccga aagtaaattc ccaaatatat
      541 tgcgcaaagc agctcccaag aactcgtatg cttccatatc ctgtatgaaa catcataata
      601 gcccagatac taataactca gcttgcaaca agcaatcatt tcattcctat ttttctctat
      661 agttttacaa ttggtatgaa taagaaatag aactgaataa ttgtcgaaat aaaccaaagg
      721 gacccagtga ctcctcatat actggtagaa aaattttaaa taacaaaaca tgagacaaat
      781 atacttttgc agcatttaaa tcttgtgtca tataaggtag taacactgat accgtaatta
      841 cgataatata cggaatataa atcttgataa atcgtttctg taaaaattca cccaatgtaa
      901 taggcttttt taaatatgac agatatagcc caaaacctga gcaaaagaag aatatatgca
      961 ctcctgtatc accaatagat attgctttgt tcagtattgc tggcaaatac gattgctgaa
     1021 aatagtacat caatacaata gcgaatattg aaaacgcctt ctcaaaacta ataatatcac
     1081 gtttatttct ttccccctca ttttattatt tcaatagttt tattcatatt ctactccttt
     1141 catctctatt gctgaataca ttattaaaac tctaatatct cttatttctc ccttactgct
     1201 ttgatcggtg cttcatcagg ctgctgtgtc tctgttaaca cttctttgcc ttttcgatcc
     1261 acacagtcgg cccctttacc tcaattatgt aaacagaacc gtaaagcagg aaatttttat
     1321 taaacataga tgttgctact tcggacattg acactcagat ctttttcata gtattactta
     1381 aacatttcag ccagttcagc agccataaca gcttccatta cttcggcagc ttccttgctt
     1441 tctgcgctaa cgctgatata ggtcttcagc ttcggctcag tgccggaagg ccgaaccact
     1501 acagagcagt tgccctccag cagatatttg agaacatcag acttcggcag gccattcagt
     1561 cccgtgctgt aatccaaggt ttcaatgacc ttcttacctc caatcatatc aagaccttta
     1621 tggaattcaa ccataatttt ctgcatcttg gtaaaacctg ccgcaccctc aaactcaaag
     1681 gaatgcaggg tgttcaaaca gtagccatac ttagcataaa tctcgttcag cttatcaaac
     1741 agactgatac cctgagtctt gtagtaagag aacatctcgc agatcagata agcggcgttg
     1801 acaccatcct tgtcacggac atagctgccg gatagatagc cgtaggattc ctcaaagccg
     1861 aaaatatagc tgtccacctt gcctgccttt tccagcatac caatctgttc gccgatgaac
     1921 ttgaagccgg tcaacacatc gacagtacgc acgccatagt tggaagcgat ccgctcgccc
     1981 agatccatag tcacgatggt cttgaccatc acagggtcag tgggcatctt gccgtgctta
     2041 attctctgag agcatatgta gtccagaagc aacagaccag tttcgttacc ggacagcagg
     2101 acataatccc cttctgcatt cttcacggcg atacccacac ggtcgcagtc agggtcggta
     2161 gccagcagca gatcagcatt atacttcttg gcatactcca tgcccagaga catagcttca
     2221 ttaatttccg gattcggata aggacaggtc gggaaattac cgtctggcag acgctgctct
     2281 tcgacaacgg tcacattgct gtagcccatt tctgccagag tacgggtaac aggcaccaga
     2341 ccggtaccat tcaaaggaga atacacgata gcaacattct tgtccacttc ttcgccaaac
     2401 agaaccgact ggccctttac ttcttcaatg aaagcagtca gaacctctgg ctggatagaa
     2461 gcgattctgg aagcgtcgcc gcccattttc acatctgcaa aaatatccag cttttcaatt
     2521 tcagccagaa tctcagcggc agcctcggtg gtgatctggc agccgcctgc gccatagacc
     2581 ttatatccgt tatacttgga aggattatgg gatgcggtga tcataacacc ggcagaagcc
     2641 ttcagataac ggatagcaaa ggatacggta ggcacaggca gcagctccgg ccacagatgc
     2701 acctgaatac cgtttgcagc aaagatgtcc gcggcaacct ttgcaaatac atcggacttg
     2761 attcgggaat catagcccac gacgacagaa ggggtttcat aggtcttatt cagatagtct
     2821 gacagtccct ggcttgcctt acctacggtg tgtacgttca tacgattagt gcctgcacca
     2881 atggtgccac gcagaccacc ggtaccaaag gacagatttt tatagaagga gtcctcaatg
     2941 tccttctcgg aatatgaccg cagttcctct acaatttctg cattggcatt tgtaagccaa
     3001 tgataataat ttgcattatg atccatagaa tctctccaat cttttaaaat tctgctcatt
     3061 tatttttaag gttaggatga atatatcagc tcaaataata tataacccca ctaatgccgc
     3121 cacccaaagt attataaaat aaatctgaca attggaatgt ctctatgtgt aaaaataact
     3181 gcaaaaactc tataagacac gaacataaaa aaacaaattt tataatctct gataaatttt
     3241 tccaaaatct ttttctgaag ccttatacca cattactaga attataaatg gaataaacag
     3301 aataacattt tcaaaacatt ctgtagttaa tctaatctcc cctgtcgttt cattcacaga
     3361 gtaaatccac caactaccaa acacattaga caaaggattt agccaaacat atctatccat
     3421 cagtgttcta aaaagaatca tatttgtaaa aaacacaagc aaaaacattt ttctaaatga
     3481 gcttgatttt ctgaaatacc gaatccagac aagaaaagat tttttcaaac ctaatcctga
     3541 tttctcctca gccatcgcaa acaagtataa aaacatagcc aaaactgcaa ttaaaatcga
     3601 aacccaaata tttgatacaa tgcagataac acattaacca aaatagaaga tatgattttc
     3661 ttcataaact ctcctttcat catgcgctaa atgagtacca tcaagaaggt ttcgtaaaag
     3721 gaggtaccca ctctaacatg aataacctcg gccagcgaaa ataaaactca acagaattcc
     3781 tatctctatg ctgctataat ttcaacaaat ttacatgtac cagttactaa tctaatataa
     3841 ttttttcagc aaggtaaatt tcacactaca cgcatacaca aaaactgtag aaattaaaat
     3901 tattatcatt ccacaagtca tttctattaa tgaatttgta atccccaatt ttgggaaaat
     3961 aaaatttaaa gcagctatta tatacggatg taaacaaaat aataacgtac taattcttcg
     4021 cagatgtact gttattttta taaatgtact atctttaggc tcagtgatac tgatacaact
     4081 accagtgcta atggaatgga aaatattaac aaattattga cttctcgcaa agctatttga
     4141 tttatgatga aactttcacc aactaataag catcctatag ctaaaacagc acataagata
     4201 aaattacggt atttaaagac cttattaagt atagtgtttt tgtggaaggc taagtgttgc
     4261 ccaataatga cgcatggtac acctataaat aatccattac gagttgttac aaacacgctt
     4321 ttataacaat atctaatata ctcgatggaa tcattcccag ataatataaa ccaataagat
     4381 tcaaatgcta aacctactac gcatagcaac aaaccactga gaaactttat tttagaaacc
     4441 tggcttttgc gtactaacca tagagcaaaa gccagtgcag gcaagaacca caggtgatag
     4501 taactaccaa taaacagaaa atctctgata tacaataata catctgtttt ccagctaaaa
     4561 tctgactgta cccaattctt taatactaat ggaatatata atatagacca aataatgtaa
     4621 attttaagta ctctgtagat ttgtttagat aatttagcgg atgtaacttc accactacac
     4681 aacaaaaagg aactgatgat gaaaaaagta ggaacggcaa ccgtactcag tcctgcaaca
     4741 ataccaatac taaatgctta atctggagct aaatagtttt gacctaaatg acgaacaaca
     4801 actaaaatag ccattagtat ttttagaata tctatcgaat agtactcccc cacacacgca
     4861 gctttataat taacattttc tatgattata ttttccctat tcatttaaga acacatcgag
     4921 ctcttctgga gtaccgagta cgtgaacttt tgatttatct actatactaa tggttacctt
     4981 catgcccttc tctatcatat aattatatag cggcgcaata tatttctcat tcttttccag
     5041 atgcctttca tctgcataat attcttcgta gagtgatgca tataattttg cagaagaaaa
     5101 ataatatgca cctaaagtac aattgttaga aattctaact ttttctctta cttctactac
     5161 atcaccatat tcgttcaact tggcaaaact ccaatgatca ccgtccgcat ggaaacacgg
     5221 aatatgtcct tctccagaaa tatcaacgta tttcatctca cctggctcaa catatgtatc
     5281 tatgttataa accataattg cattatcagg tgcgcaataa ggaattgcaa gcatacatgt
     5341 agtggcttgt ccatcagtca tatagtcaat ttcaactacc tcgacatttt caattccata
     5401 cagactgcaa tgttctttga taaaaacagt agcatcatct tccttacgca caacaaaaac
     5461 atacttttct acatgtgaat tataatcaag caagctgtct aaggaccaat caaataaggt
     5521 tttacctttt gcttcaatca taaatttggg cacagtgtag ccaactttac gaaagcgact
     5581 gccaagacca gccattgtaa taataaccgt catcttattc actttataaa gtctccttta
     5641 ctcagatttc aatttggatt ttttgcaaat ctcatcgagc tgatccaggg tacacgataa
     5701 aaattcatcc ggacgaactg tgcgatcatc aacataaaac cctttatggc ctggccaagg
     5761 ctttccataa agaatttcat catacggaat ctcccactta tctagccaag ccaacaagat
     5821 ttttgcagta tttgcattga tcacacctaa attaccatga taagaattca tattgcgaga
     5881 tgtgaacagt gtaatctttg ctccgttctc cttatagtat cgcaacttct ccaccatatt
     5941 agcatacgga actaaatctt catatttctc atctttcttt ttgatggggc acaaagttcc
     6001 atcaatatca aaaacaaatg cgtaatcttt aaacatataa ctaagacatc tcctttattt
     6061 tagccaggca atttatatgc ccggctttac actgatcaaa tagaaaaaca atagctcaaa
     6121 gcattaacaa taaatatccg ggacaactcg tcctaaaatt tccaaaccag ttgctaacat
     6181 agccatctga tggtttaagc tttctccatg caaaggaatc atacttaaaa acagcaaagc
     6241 ctcaataagt tcaatttttt tcaaatcaga acctatttca tctttgaaga cagagtaaaa
     6301 tacttcatat aaatcataat ctcgtttacg atcttgaaca atatagtcta tgattgcctt
     6361 ctttggatca tattttattg taaacaagtc cttaattata aagtcatatt ttccatcaac
     6421 actatgaaac aattttgcca gttcatatct ataatcacca taaatatcaa aatctccaaa
     6481 cttacctcgc ggatctatga ctttaatgaa cgaaaatgtg ttatctacca taatattggc
     6541 aaagcataag tcaccatgaa taatattaaa ttgcgtaatg tcaaacaatt ctcttggaac
     6601 acttacgctc aataaagctt ctatctggtc caaagattta tatctgactc catttatttg
     6661 aatatcagaa gaaaagaatt ctgtgaaccg ggggtccttt ctgagcttat tgaatctctg
     6721 aaatgtttta tcgagataca tatctttcaa tgacttttga atattatccc ctgaaacaga
     6781 gtatctctta aagtctgagc atacaaatcg tattctatta aagatatcta tccattgttt
     6841 ttttgttaag tcactataga gaaataactc gtgaacagta tgatacgaat agtattccat
     6901 acttacatat ggattaatat acgaagtaga atattcgaat attctaggtc tcacatattc
     6961 tacctgtgct ggcaacttaa gataccactt aatttctccg ataaattttt caacatcttc
     7021 actaattttt ctcaggatgc tacgatcttt atcaattgaa atgtggttaa attctcttgc
     7081 cttaacttcc aatttggaat tataatattt atctgcatgt ccaatgtcaa accaattatt
     7141 tgttttaata gagagaaatt catatcgttt gctatactct tgtaaagctt gataaaaact
     7201 attcacattt actacatttg acatcaaagc tttacgcaaa cactctctaa aatactgggc
     7261 atccataatt tgaaatacac cagaaaacag tttgtgcttc tttccatcat cttcttttaa
     7321 ctcgttctta tctaaaacag aaataatatc tccgtctttt tcttcaaaga atgtccaagt
     7381 attactataa tagtcttcag catagaaaaa gctgtcacat tccaaggaat atatattatc
     7441 attgataatg gtatcagcaa aattaataat aacaggctcg tcacatccta ttagagaatc
     7501 atatatagtt cttccaagat cccctaactc tttaagctga ataatattta ctgttttaga
     7561 tttaatatac ttatctaatc gtcttgctac cttatccatc ttttcgtaac aggcaatatc
     7621 gatagcagaa catacatctt tatattgatc atataaataa tcaaatacaa ttttttgatt
     7681 aatcggatag ataattgcag gaagttttcc aagatggtga agttcttccg gtacaatctt
     7741 agcagatgga atcaataatc tcatttcacc gtgcctccgt tccattccca agatttgctt
     7801 gttaattttt tatattttta aggattttca ttatttgcgt ccttttaaga aaagccacaa
     7861 taactaatcc aataataata ctatatctta gaattgtaga aa
//
LOCUS       DAHHPA010000111         7772 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.116, whole genome shotgun sequence.
ACCESSION   DAHHPA010000111 DAHHPA010000000
VERSION     DAHHPA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.116"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(22..1386)
                     /locus_tag="NBH55_002446"
     CDS             complement(22..1386)
                     /locus_tag="NBH55_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1510387.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1383..2942)
                     /locus_tag="NBH55_002447"
     CDS             complement(1383..2942)
                     /locus_tag="NBH55_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510388.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2998..5478)
                     /locus_tag="NBH55_002448"
     CDS             complement(2998..5478)
                     /locus_tag="NBH55_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510389.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5462..5797)
                     /locus_tag="NBH55_002449"
     CDS             complement(5462..5797)
                     /locus_tag="NBH55_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510390.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5811..6845)
                     /locus_tag="NBH55_002450"
     CDS             complement(5811..6845)
                     /locus_tag="NBH55_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCD1510391.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6935..7615)
                     /locus_tag="NBH55_002451"
     CDS             complement(6935..7615)
                     /locus_tag="NBH55_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCD1510392.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         2225 a         1554 c         1420 g         2573 t
ORIGIN      
        1 aataaaagac cgtgatattt ctcacagtct tttatctact ctattgctat ttgctctctt
       61 agctagtact ttaaaagcta actcattgag gtagtgaaaa cgagaggtgt attcagacag
      121 aaatccaaaa tcctcaactg ctagctgtgg aatcgtagtt ttgtgtttca cgatatccgc
      181 aagcaggttc aaatctgata ataagaattc ttggcttatc agctgtccgc caattatttc
      241 taatgtatcc ggatggtaga tcagctttgc atttactgtt ttgttttctt cgtcgtattg
      301 tggaaataga tcaaattctc ctgaacacga accggtattt tgttccaaga atggcgcttc
      361 atcttctgtc agccctacag ttcctaaaaa gtagtctata atgactgctg ctgttatctt
      421 ttgtctgagg tcgtaagcgg ttggctgacc actcaaagtt agcgctactg ctcttgccat
      481 atgtacagct cgagtaacta aaggtaaaaa gagatgccgt ttatttttgt caaaactgac
      541 tgggacgata tctccaatcg catagatgtc cggatcactt gtctgcagat attcattgac
      601 tatcacagtc tcatcctcat acaaagccac tttttctttt aacaatgtcg tattaggacg
      661 agtttgggca gaaaaaatga ccatatctgc ctttattgtt tgagacagaa gttgtacgga
      721 tactactttc tcttctgatt ttttccaatc gatcaagaat tcatttggat aaaaatttat
      781 ttgcttagtt tgcagctgat tttgaaaact gttgatcata tccttatcga aatatcttga
      841 taaaacattc ggcatataat caactagatg aataatgtat cctttatttc tcaaagcatc
      901 tgctaattcc aatccaatgt aaccagcacc gaagatcact acttcttttg cttcttttaa
      961 agcggcaagt acttgcttag attgcactag atttttatag gtgtagacat tttcagcttc
     1021 aatttgttta tacaaggtga caggagagga acctgtcgct acgattaatt tatcataggt
     1081 tatggtctcg ttttttcctt ctttggatgc gaggatgagc tgatgtttgt ctggatcaag
     1141 ttcagttact ctccattcgg tgatcagttt tgccccactt tcttgaagag tccgcatgtt
     1201 atgggcaact tcagctaaac tacgtatctt tccttttaaa taaaggttga ttccatttga
     1261 aacatagcct agtccatctt tatgatcttc atcgatcagt aaaacttctg ctttcggttg
     1321 aagttttctt agatttaggg cagctgtgat gccttcatgg gaagcaccaa tgatcacgat
     1381 tttcatgttt tattcatcct taaattcaat ttttctaaat aacattcttt taaaaattgg
     1441 ttcaaatagg ggaaatcata attatattcg ttgctagtga atacaaaaac tttagcggac
     1501 ttctgtgacg ggtactcttt caattggcta gtcaaaatga tatcatactg cttttctttc
     1561 tcgtattcga ctaaatcata tttgatcgta ttcagcaatt gttcctggat gatttctttc
     1621 acgatatccg ccattagcct ttcataagga agatcataag caatttgcag acgtaattct
     1681 aactgcttga tcagcgtccg taaaatagat gcatagcgag caaataattc aaagcgggca
     1741 ttttcctcgt ctacttcagt aaaatcaaca atagcaaatg tttgtctgac caatctttca
     1801 cataaaacca ggaattgttt atcttctact tcagttaacc gctgttttaa tccttcttgg
     1861 ccaaaaatat tcatccatcc tctaaaatag gcaaaatgag cgtgattttg catcactgtc
     1921 aacgcaatct tttgcgaaat ctcttctgga aaaacataaa aagagaaata ttcttctatc
     1981 gattgtttaa acgttcgatt caattgagct actacgtcgt ctttgtattt ttcgcttatc
     2041 aaaaactcct ggacatgcga gttatgtgta tctaatgtaa agttactgat catgaaaaga
     2101 taaaaaacca acgtttcatt ttgattccaa ggaaaggcat attgaaagaa gtaacgggtc
     2161 aaaaatgtat ggaggtccaa taaaaattgt gtcgtttgaa acaatccaac aggaagattg
     2221 ctataatctg gtttggattt tttcgtacag cgcattcgct tcttcataat ggaaagccat
     2281 atttcaaatt tcaatcgtcc ttcttctgta atagcaaaac taaactcttt ttgcagcaga
     2341 tctaaaaaag tgtttcctga taaatcactg tattcttttt tgttttcaag atagggacgt
     2401 ccaaaccaaa ataattggaa ataaaaataa cgaatttgac tttctggacc ggtcagccgc
     2461 ccgcttttta ttttcagatg aaactcattt aaagaatcat ttaattcctt cactctgcga
     2521 gacaaagtag ccgaactgat attaagttcc atgacaagtt tctgccgatc aaatgagcca
     2581 ttttccaaca gataaagaag tatctgatat tttgtagaat agcgcaagta acaatgataa
     2641 taaacatcga ctgctgctat atttccatgt ctgattaata aaatattccc ttcttggata
     2701 acaatttcca attgtttgat attttccaag ttagcttgta aataaaacag atcttctttc
     2761 aaggtagccc gatcaactga taagcgggta agtacgttat taacggaaac ctctcctttt
     2821 tcctctaata aaagttggaa aagtttgatt tgccgttgtt catttttttc aagcaaactg
     2881 aaaagttcca agtccactcc ttcaagcatt tctgatttta acagcagaag tttccttctc
     2941 atccttgaaa aagaggatct gtgacagtta tcacagatcc atacaaattc taatcactta
     3001 gctggttggc ccagctgtca gttcccatgt gaccatgcct tgatacgttg cgccaggttg
     3061 agcattcgta ctgctattta cctctaagag taaaccatcg gtatcgggaa agtcaacgag
     3121 aacctctcct ttagctgttc catcactctc ataaatcaac gtgttttgac cttgctgaat
     3181 caattgcttt gatccagaat gattgaagta taattttgtt ccgcttaatt cttgaccttg
     3241 atcattctta aataaagaag tcaatgtacc tgtcagttgc caaggagacg tgcttggaag
     3301 ccgagtatcc cgcacatcaa aagaaaaatc gccttttgat tcataataac ttgtcttcaa
     3361 cggtttgggg agatcgttac caaatgtaaa atcatctggt acattaacta atgttaattc
     3421 ttgtttgggg acaagaattg ttttagcaga tgtttggaca actgggttag gaagcttagc
     3481 accatctgct aatgtaatat tgctggaaag tacagctgcc tttgtcagta tgctgacgga
     3541 ttgtaaagaa gtatctacag tatttaattg atagttgtaa gtaagatgaa tggtatctcc
     3601 tattttgctc aatagatctg ctgggacaga aactccttgt tgaatgccgg tatctgcgat
     3661 agttgtacca tttaatttta aggaaccatt gatataagta gtattttcag gaagtgagat
     3721 cgtgattttt ttgtcggtta tcgcatcccc agattttgtt aacgtgaatg tcacattttc
     3781 ttgaatcgta tctcctgata gagcagacag gggatctcct gtctcaggtt gtctgctaag
     3841 gttttttagc tgactatctg cactcacaga tggaaaatta gccactgtta tttgaggaag
     3901 agtggtgatt ccagcttgtt cagactgcca tttgtacgca aaagacggat acatgttagt
     3961 aagtacatcg tccaccaagc gataatcaaa ataatattgc cctgaagcca atgcaggtag
     4021 tgtagaagta gctgtattta cacccccgtt atttgattgg atcgtttgga aaagttgatc
     4081 tgtccatcct gtcgctaatt tttgggttcc tttcgaataa agcctgtaat ggatcgaacc
     4141 cttttttagt tcagttgctg accaattggc cgtgacttgg tacgatgcag aagaaaggag
     4201 ctttccccac tgactatcgg ctgttaagtt tgttggtaaa accgttccgt ctagcttgta
     4261 ctgctccaat acctttaaag caacaggaga aagcaatccg ccttgcgcat ctttcccact
     4321 gtacacgata ttggctgtct gattcaatcc acttgtgctt gctggagtat gagtcagtat
     4381 tgctccttgt gtaacgtttt ttcctgtaaa atagttgact acgcgatcaa agcgactaaa
     4441 tggcaactga tcaaaacgtc cttcagtcag ccccatcgtc aatgtccgac tattggtaga
     4501 gcctggaagg ttctggttat ttccatccac gtattctgtg gtatcagcaa atgtattata
     4561 ttttcctgtt gaatcacttg gtgagatgta actattccac gtatttatct ctgcacttgc
     4621 agaaaatgca tatgctcctg gattcaagtt tccgccagtt aatttaggat aaaatctacc
     4681 tgcagctgga gctaaaatcg aattgaacat acgatcttgg ataatattat tcgaatcata
     4741 aaaatcaaca gaaaaaacgg cctttttaga catagaaggt tgtaatttac ccttggtcaa
     4801 aattttgttt ggactcgtat catatttatc tccgttttct gtcatcttcg cgacattagc
     4861 tgtaacatca aattgaagcg ggatatcgtc cacgttgaat tcgcctcgcg taataatagc
     4921 aataatatct gtcaatagag tatttgtatc ccacgacccc atcccaaaac gcaaggtatg
     4981 gcttcctgct gataataaac ttccgctagt aaccttgatg ggggcaccgg caatatacaa
     5041 gtaggcgttg ttccagtcaa ttgcttttaa tacatcactg cttgatacat gttccggcaa
     5101 attgatgatc gccacaaatg caaatccttg tgcagtgata ttcaccaaat caagtacatt
     5161 tgttagtttc acatggcttt cccaatgaaa acggaccgtg ccacctttcc aaggatcatt
     5221 tgaaccatcg ttcaaataag tagcagtcaa tttaggcaat tggttagccc cagttagttg
     5281 gacaagcttc ccgccattga ctgcactacg tccaccattt ggaggtacga tattttcata
     5341 cgtgtatgga tcataagaaa tggcaggagg cgttgctcca acagcttctg ccgaaggtga
     5401 aaagataaat atacttaaaa caaaaagcga agtaaaaaag aaggagcgaa aaaatgaaaa
     5461 tttattttgt agcaacatgt tcatcctcct tttcctttct tctccataac aacaaccacg
     5521 caagaagcca taattctata ccaatgatca aataaagaaa acggatcttt tgttcattcg
     5581 tctccgggaa tacgccatgt tgattagatt ctttataaat cggtagctgt gtctgctgtt
     5641 ttgatgcgcc attgactgaa ccacttggat gcggaattgg acttagatca taaaatgtaa
     5701 tcgtacctga acttttggta tctgcttgga ctggctcttg ccagctgaag aacagcaaac
     5761 ttgcaataat ccctcccaat aagatcgatg ttttcaacga aatcacctca tcatgttgtt
     5821 tctccttttt tctttttctt gtggacccac aaaaacagca aaatcaacca taacactaga
     5881 actgcagcaa ccaatcctgc ataaagccat gggaaagatt gttcttgtac tgcaaaaacg
     5941 gaattttcat ttactttttg tgctttttct tttttgatcg taaagttttg tgtaaatcgc
     6001 cattcattac tgtcgcctga ttttgctatg atttctgcgc ggtaatcgcc ggcttttgct
     6061 ttatcaccag ctaaatcaag attcaagtga aaattcgaat ttggtgcaac taaaaatgca
     6121 ttagacgtat tttcaatata aagctgattt tcataaaaga tcttgctcgt aaatatcaaa
     6181 ttgctgataa tcctaggctg aggattttgg aaacgaagct gaacagttgg ctttgcgttg
     6241 acctcgtcca aatctcctga taaaagtttc agatccggtt ctatcgtctc ttgactttct
     6301 tgtaaaacga ctgcgagatt ataagcaata cgatttttga tttgtgctcc ttctgtagca
     6361 ttttctgttt cagcttcttc tttaggtgaa acatgaatac ccgctaatac acgtccctta
     6421 aagctttttg ctgggatttt cacttctata ggtacagttg tttctgcatg tgctgataca
     6481 ttgatttcct ttttcgcaat gagcaaaacg tcacggacat caaacggcaa tgtctcatct
     6541 aacgttgggt ttttttgact ataatcgatc gttccaccat cactcgttac cgccggattg
     6601 acagagacct caaatgttcg ttcttcatct gaagaattgc tgataattac ttctagtgtc
     6661 tgattttgtt ccggtgccac taataaatca aaataaccga ttgctttatc tatttggttc
     6721 tctggaaata ccggtttgat tccaaagtct cctgctgaat cctttgcttg gctgattgac
     6781 ggaaaaatga aaaatagact taaaatagaa aaaaacgaaa caaacagtcg tttactttga
     6841 ttcattgtcc accacatcct ttcaacaaca actgcggtaa aaaatccttc acctatagta
     6901 ttttcaccgc agtttgttga atttatttca tactctatgc tactgggcca gccacgaggt
     6961 tccaagtcaa atcagccgtg aatgttccag ctgctgcttt cggcactttc agtgtgatat
     7021 ttgctggatc ataaacagaa agccatgttc ccagaccttg atcttttgct gctgtaaata
     7081 ttggtgtcgc tgcacttgct ttactcaacc cagttacagt ttgactagtt ggtccagtgg
     7141 attcgttatc tgacgtactt ttaacagtgg atgttccaaa atctaattca gcgccttgca
     7201 aaacttgtga accgtttaca aaatctgaaa ccgaaactgt cacgttccac ccttgtgctt
     7261 ggccatctgc acctacacct cgacgatctg atacttggag ataaggtgta tcgttttttg
     7321 aagcgtctac ttgatacgtt tttgttccgc tttcaatctc gtgtgtacca aatggcaatt
     7381 cgggaactac atccagtgtt aaaggacctg tctggccagt tgctggattt tccggatctg
     7441 ctggctctac tggttttgaa ggatcagtag ggttaagagg ctccacggta tttgttggtt
     7501 cagtaaaggt aatatctcct tttgaagtcg cttgaccctc agcaaaagct ggtgttgcca
     7561 agccgcctag aagaagtgtt gaaagtaata atgtcccaat catgttcttt ttcataataa
     7621 atcactcctt tttcttagtg acctaagtat agtagagaca gtatgcaaaa aatcctcatg
     7681 aattcatccg cttacttgaa attatgaccc tttttgtata aaagagaaaa aaacaataaa
     7741 atcgtttaca tgaatattct tcctttttta tt
//
LOCUS       DAHHPA010000112         7627 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.117, whole genome shotgun sequence.
ACCESSION   DAHHPA010000112 DAHHPA010000000
VERSION     DAHHPA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.117"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(16..708)
                     /locus_tag="NBH55_002452"
     CDS             complement(16..708)
                     /locus_tag="NBH55_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD1510393.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            703..969
                     /locus_tag="NBH55_002453"
     CDS             703..969
                     /locus_tag="NBH55_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1510394.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKSFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1145..1714)
                     /locus_tag="NBH55_002454"
     CDS             complement(1145..1714)
                     /locus_tag="NBH55_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD1510395.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="NBH55_002455"
     CDS             complement(1788..2111)
                     /locus_tag="NBH55_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510396.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="NBH55_002456"
     CDS             complement(2265..3077)
                     /locus_tag="NBH55_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510397.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="NBH55_002457"
     CDS             3431..4279
                     /locus_tag="NBH55_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCD1510398.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="NBH55_002458"
     CDS             4424..5365
                     /locus_tag="NBH55_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCD1510399.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="NBH55_002459"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="NBH55_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2274 a         1512 c         1371 g         2470 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacctattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 agttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 aacttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgagaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg aatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaac ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctcgaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacct
//
LOCUS       DAHHPA010000113         7553 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.118, whole genome shotgun sequence.
ACCESSION   DAHHPA010000113 DAHHPA010000000
VERSION     DAHHPA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.118"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(896..1309)
                     /locus_tag="NBH55_002460"
     CDS             complement(896..1309)
                     /locus_tag="NBH55_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCD1510400.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1765..2250)
                     /locus_tag="NBH55_002461"
     CDS             complement(1765..2250)
                     /locus_tag="NBH55_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510401.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2389..2634)
                     /locus_tag="NBH55_002462"
     CDS             complement(2389..2634)
                     /locus_tag="NBH55_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCD1510402.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2827..3627
                     /locus_tag="NBH55_002463"
     CDS             2827..3627
                     /locus_tag="NBH55_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1510403.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3646..5514
                     /locus_tag="NBH55_002464"
     CDS             3646..5514
                     /locus_tag="NBH55_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCD1510404.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5507..5998
                     /locus_tag="NBH55_002465"
     CDS             5507..5998
                     /locus_tag="NBH55_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCD1510405.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEEGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5986..6540
                     /gene="srlA"
                     /locus_tag="NBH55_002466"
     CDS             5986..6540
                     /gene="srlA"
                     /locus_tag="NBH55_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCD1510406.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6558..7553
                     /locus_tag="NBH55_002467"
     CDS             6558..7553
                     /locus_tag="NBH55_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCD1510407.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     EKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2690 a         1210 c         1434 g         2219 t
ORIGIN      
        1 agatcttttt atacttcaaa aaagataaca cccaactttc aaaaatatgt tttggaagtt
       61 gggacaaacg agttaataca ttttaataca ttatttttta ttcaaaaaaa atagcacaat
      121 ggcaaagatc gtaagagaga tcataagccc caatgaatga ctaaaaagaa aagcagaatc
      181 acatgccata tcatcccata tttcttcaag aaaataaata agcgggaaaa agggaaggaa
      241 tagtagaaca tcgatccagc accatttttt tcttcttttg aaataacata aaacaataaa
      301 catgaacgtg cagggaagta aaaacaaagc tttactcata actaacaaaa taccagctcc
      361 ttttattttt ttctccccca cattcaaaga gtaacaatgg tatgtactta ataatagaaa
      421 aatggatcaa cttgtatgaa taacagctga aaagatctgc accaaataaa aaacttccag
      481 ccaaaactga ctggaagttt atttttttct gaaatattct aacacaaata tgaaaaagat
      541 ggccgaagct aataagccga tttcgtagca aaacaatgag ataccagagt cgtaataata
      601 aaatcctcta atcttatgga taaaatagat catcggacaa gcagataaga gtaagtattt
      661 gttaggcctt ttgaataagt aaaagaaagc actggtcatt atgataaatg gtatccaaaa
      721 caaaagttta cttaagacaa tcaaatgatc aactcccata ttttttacca agagagatca
      781 taacatcttt atcgtaaaaa caaaatattt ttttgctcat tttgaaaatt taaacatact
      841 cgttcttatc caaatatata aaaatcgccc ctcattcatc gagaggcgat aagttctaat
      901 ttaactcaat tttttttgtt tcttttggct gataagctgt tttaggtaat accaatttca
      961 gcacaccgtt tttaaaggtt ccagtgattt tttcttcatc aacattagga agatagaatg
     1021 aacgtttgta agagaccgaa tgtctttctt tccgtacata gttgccatct tctttatctt
     1081 ctgtatggga ctcatgattg gcggagatcg tcaaagtatt atctgaatat tcaactgtcg
     1141 tgtcttcttt gtcacagcca ggcatatcag cagttagttt gtattctttt tcattttcga
     1201 ctaaatctac ttttggataa cttgaaacac ctaaaaaatc attgaatgct ggactaaaca
     1261 cgtcattgaa atcagggaac atgtcgcgaa tgctaggtac atttgccatt tttgatcatc
     1321 ctttcaataa gtatttttta tttatcccta tttacgtaat aactctattt tgaaaaaaat
     1381 gcaagtataa tggttagctt tgtgaatatt tcatctaata cgatacaaag gctatctttc
     1441 taatttgaaa aagggatcat taaaataata agaagaaaat atgtcaaaag gtaaaaaagt
     1501 aaattcttct atccaaatat agaaaaattg aatctagaaa accgcccgac aataaatttc
     1561 attaactcag ttgaccgaaa atgaagtaga taccaaaaat ttatcaagag agcaagtaga
     1621 gaagtgagta tattcataag agaaatagct gtaacgaatt attaggttaa tgcaaatgct
     1681 tatttatact ccgtataact ttctcagaaa aaatgatacc aataatgact tgctagtact
     1741 gtaaatccgt ataatatcat ctaatcaaaa tggtgttcca tttgcatctg tcatcgtaat
     1801 cgatccttcg ggtgacactc gataaaaacc aacgcttcca gatcctcctt gggaacggat
     1861 ttgtttgctg gaagctgtga acaaataatc tcgatcaatt ttttgataaa agctaataac
     1921 aatatcgtca ctttgtaatt cggaacggtg ggcaataaga aatagaagtg ctttgtcaga
     1981 gttttctaat ggaagagaag gtacagtgct ttcttgcatt tcagatgttg aagaaatgct
     2041 aaacgactga atacttgatt ggctatgttg cgaatctaca ttaccagtag aagtattagt
     2101 aatggaagta gtagaagtac ttttagttga accaacctca gttgttgaaa tggctgctga
     2161 agattcttta acagaattct ttgttttata tccacaggca gaaaaaagta ttacagttcc
     2221 caacaagcct gcgtaaatcc ctttatgcat gatagctcct cctaaaatag attatcttta
     2281 ttcagtatat cacttaaaca aagaaaagca gatggtttaa aatgaaaaaa ccttcccaat
     2341 aaagggaagg ttcatgaaga taataacttt tttaataatt atagctttct aatatctttc
     2401 atcaactttt ctagtaatca ttgggaaaaa tttcaagcag tagatcgcac aaatacctac
     2461 gacgatataa atgatcttcg caaaaatagt tgttgaacca ccagcaatca tagctactaa
     2521 atcaagttcg aacaagccta ccaacagcca gttcaaccct cccacaatca ataaagttaa
     2581 tgcaatagca tctaagtttt cattagaatt ctcctttctt aaatgataaa acaattatat
     2641 atcaaaaaga gaaacattgc ttatataacg ctcaggaatt ttagcaatct atgcaaaaag
     2701 atgaaaattt tttttgctct tccattagta cacatttttt agctgaagct gtaaatttca
     2761 tgtcttaatt ttttttagtg agcttttata ctggatttgt aagaaaaaag aaggagagga
     2821 tcacatatgt cggattggtt ggatattgca ggaaaagtag taattgtaac tggaggttcc
     2881 tcaggaattg gaagaagtat cgtcgaaaat ttgttgaagc agaatgcaca agtagcgaat
     2941 tttgatgtaa ctgaatgccg tatacagcat gaaaacctac tatctttaaa agtcgatgtg
     3001 tcttctaaaa cggacataga agaagggata tataaagtaa tgaaacattt caagacgatc
     3061 gatggtctag tcaataatgc aggaataaat attccttctc ttctaataga tagaaatcat
     3121 ccaaaaagca agtatgaatt atcagaacaa gtttttgata agatgatcgc tgtcaatcaa
     3181 aaaagtgtat atttgatgag ccaagcagtt ggaagaatct tggtgcaaaa gggatcagga
     3241 gtgatcgtga atctttcttc tgaatcagga ttggaaggat cagaaggaca gagctgttat
     3301 gctgctacaa aagcagcgat gaacagtttt actcgttctt gggcaaaaga gttaggaaag
     3361 aggaatgttc gtgtggtagg tgttgcacca gggatactgg aagaaaccgg tttgagaaca
     3421 caagaatatg aagaagctct ttcttatacg agaggtataa gcgtcgaaca gctgagaaac
     3481 ggttacagtc gaacaagtac gatccctcta ggaaggagtg gtaagctgca agaagtggcg
     3541 gatctagtct gctattatct ttcagaaaga tcaagttata tcactggagt cacgactaat
     3601 atttcaggag gtaaaacaag aggataaagg atagggggga cgcacgtgac gctagtaaac
     3661 agatggtacc aattattgaa attactagtc actcacaaga aagtttcaat ccaggaagtg
     3721 caaaaagaaa tcaaaaccag taatcaaacc ataaaaaaag atattgggga gttgaatgaa
     3781 gaaattgccg gtattgcaaa aatcatccaa aaaaacaaac attttgaact tgaaatttat
     3841 gattttgatt tttttgatga gatcatgcaa ggaaaattga aaacagccag tgatttcaat
     3901 tcggcatcca aaagaatcgc ttatttacta aataggctga tcgaaacaga ggatttctta
     3961 cgtatggatg atttgtctga agaagtcggt gtcagcagag gaacagtggt caaggatcta
     4021 aagacattaa aagagatgat caatgatttt gatgtaaaga ttacaggcac accaaataaa
     4081 ggactaagaa taataggaga tgagctagaa ttacgactac ttttatttta ttttgtttct
     4141 ccctactttt cagagacgaa aacagaacag tttctagaag aaaaacaagc aatcaaacat
     4201 tttcagaaga agacgaatgc tagtaaacaa gaaatcaccc ttttaacgaa agttatcggt
     4261 atttctttga atagaatctc tagtaactat ctgttgaaga agcccatcaa tcgttacagt
     4321 gggtgttttg aatatatcca agaattcaat gaactgatca atcagttgga agaaatctat
     4381 gaactaactt tgagccaata tgaaaaagat tttatcagtt ttcctttgaa catcatcaac
     4441 acaagtataa cgatgataag gaaagctaac tctgatttgc gcttatattt cgaccccatg
     4501 atgatgcgta ttcgacgatt agatctcatt gatctggatg agtccttctt atatcaagag
     4561 atgaaacagc acctctcttt attgataaat cgggtaattt ttcgttatcg attgactgat
     4621 ttattttatg gtgagataga aaaaaagtat cctttttctt accaaatcgc tagcgaatgc
     4681 atacaagcat tagaagctat cttgcaacat aagatctcta aagtagagat cagctaccta
     4741 acgttgtatt tggagttagc atttcgatat caaaaagagc atttttcctc taaaaaagta
     4801 gccattgttc atacgacagg aaaaggaaca gcattaatga tccaaagaca aatcaaaagg
     4861 atattgggga gggatataca aataacacat gtttcagaag aacaatatga aaaagtagat
     4921 ctaaacaaat atttcgctgt ttttacaacg attccgttaa aaaatgtaaa tgaagagaca
     4981 ccggtgatcc atttgactaa tctttttgat gactcttggt taagggcaca atggcaaaaa
     5041 gcaaaaaaaa agcgattagc cgggtttcat tctactgttt ttttctatca aatatggaaa
     5101 gaaaaaaaga cgtatcaaga atatttaaac aaattagtcg ctcctttgat cagaggctat
     5161 tatgtggatg aagaatttga aaaaaggatc ttgaaaagag aatatcagca aagcacattt
     5221 gttaacaaag aatttgcttt tcctcacaca accaaccagc tatctcaaca cattgtttta
     5281 tcagtagggc taataactga tcaaggttta gcagaaacag gaggaaatgg agttattttt
     5341 ttatttgcca tacctgaaga aatgacggct gaagttgaga atgaattatt tgagctatac
     5401 gatatcctat ttgaactcat aaaaatggga atagatgagt ttattgaatt agcaccgaca
     5461 caagaatcat ttttaaaact aatcaaaagt aaaggagaga gacttcatga atgatatgta
     5521 tctattcata gttttcgcag cttcagctta tgtgctgcaa gcagttatca gttggaagca
     5581 actaaagcat ttcaatcaaa catatagcga actgcgaaga aaaggaaaag tagcgattgg
     5641 taaacgacct gggaaaatcc gttcaggcac gattgttatg ctagctatag acgatcatga
     5701 tcaagtttta gaagtcagaa aaatgcaagg ggttacatct ttggcaaaat ttcattcatt
     5761 acctgtctat gcgggtgaag atatccatta catcgatcgt taccatccct cagttcgagc
     5821 ggaagatcgt ttgacacaag cagctttaga aaatgccaga gaagtatact tacgaataga
     5881 ggaaggaaat gaaaaaagtg aacaaccttc attgcctttt attgctatgc tcaataattt
     5941 tcggatatgg aaaagtacga tcaaagaaaa aaggagtgac aaacgatgga tatcataaca
     6001 aaatttgctg agggatttat gaggctgttc caaacaggtg cagaaacatt cataagctgg
     6061 atgacaggaa tcgtaccagt tgtcttgatg ctgatggttg caatgaatac gctgattgct
     6121 tttttaggtg aagaccgagt aacgaaagta gccaaattat cagcgaaaaa tccgcttact
     6181 cgttaccttg tgttaccgtt cttatctgct tttatgttgg gaaatcctat gtcatttaca
     6241 atggctcgtt tccttccaga atactacaaa ccaagctatt atgctgctca agctcaattt
     6301 tgtcatacga gcaatggtgt attcccccat attaatcctg gagaattgtt tgtatggatg
     6361 gggattgctc aagggatcga aaccttaggg ctaaattcga tggagctggc tatccgatac
     6421 ttgcttgtgg gactgctgat gaactttatt ggtggttgga taactgattt tactactgga
     6481 tttgtatgca gacagcaagg aatcgttctt agcaaaaaag tagagctttc agttgattag
     6541 agaaaggaga gttttaaatg tatggtgata ttaagatcgt aagaggaaac ggcgggtatg
     6601 gaggtccttt gattattagg ccgacaaaag ataaacacaa gttcatctat gtcacaggtg
     6661 gaggtgagag gccaatgatc gtagataaaa tagaagaact gacaggtatg gagtctatca
     6721 atgggttcaa aacatcaata cctgatagtg aaatcgcgct tgcaatcgtt gactgtggag
     6781 ggactcttag atgtggaatt tatcctaaaa aagggatacc aaccatcaat atcgttccca
     6841 caggaaaaag tggcccatta gcacaatata tcactgaaga tatttatgtt tcagcagtag
     6901 ggttagaaca aatacagcca ctaatggaaa ctgatgataa caaagaaaca gtaaatactg
     6961 catctccaga taaaaagtac aaatatgata cttctaaaaa gataaccgag caaaaagcag
     7021 agcagcaaaa ctggatagaa aaaattggaa tgggcgcggg aaaagtagtt gctacattca
     7081 atcaatcagc aagagaagca gtagatacga tgatccggac agtgatccct tttatggctt
     7141 tcgtttccct tttgattggt gtgatacaag gttctgggat cggtaatgta tttgctaaca
     7201 ttatggcacc tttagcagga aatgcagttg gattaacaat cattggcttt atttgttcac
     7261 tgccattttt atctcctttg cttggtccag gggcagttat tagccaagtg attggaacat
     7321 tgatcggtgt agagattggg aaaggaaata ttcctcccca attagcatta ccggcactat
     7381 ttgctatcaa tactcaaaat gcatgtgatt ttattccagt cggtctaggt ttggaagaag
     7441 caaaagctga gacagtagaa gtaggtgtac catcagttct ttattcaaga tttcttaacg
     7501 gcatcccacg agttatcatt gcttgggtag caagttttgg attatatagc tga
//
LOCUS       DAHHPA010000114         7364 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.119, whole genome shotgun sequence.
ACCESSION   DAHHPA010000114 DAHHPA010000000
VERSION     DAHHPA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.119"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="NBH55_002468"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="NBH55_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCD1510408.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="NBH55_002469"
     CDS             complement(1121..2497)
                     /gene="trmFO"
                     /locus_tag="NBH55_002469"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCD1510409.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(2466..4544)
                     /gene="topA"
                     /locus_tag="NBH55_002470"
     CDS             complement(2466..4544)
                     /gene="topA"
                     /locus_tag="NBH55_002470"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCD1510410.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="NBH55_002471"
     CDS             complement(4666..5523)
                     /gene="dprA"
                     /locus_tag="NBH55_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCD1510411.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(5579..6346)
                     /locus_tag="NBH55_002472"
     CDS             complement(5579..6346)
                     /locus_tag="NBH55_002472"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCD1510412.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="NBH55_002473"
     CDS             complement(6339..7199)
                     /gene="ylqF"
                     /locus_tag="NBH55_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCD1510413.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         1904 a         1631 c         1289 g         2540 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgccagaaaa
     1081 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
     1141 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
     1201 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
     1261 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
     1321 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
     1381 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
     1441 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
     1501 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
     1561 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
     1621 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
     1681 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
     1741 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
     1801 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
     1861 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
     1921 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
     1981 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
     2041 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
     2101 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
     2161 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
     2221 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
     2281 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
     2341 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
     2401 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
     2461 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
     2521 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
     2581 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
     2641 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
     2701 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
     2761 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
     2821 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
     2881 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
     2941 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
     3001 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
     3061 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
     3121 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
     3181 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
     3241 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
     3301 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
     3361 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
     3421 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
     3481 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
     3541 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
     3601 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
     3661 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
     3721 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
     3781 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
     3841 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
     3901 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
     3961 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
     4021 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
     4081 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
     4141 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
     4201 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
     4261 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
     4321 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
     4381 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
     4441 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
     4501 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
     4561 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
     4621 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
     4681 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
     4741 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
     4801 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
     4861 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
     4921 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
     4981 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
     5041 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
     5101 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
     5161 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
     5221 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
     5281 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
     5341 ttcctgccaa cttttgcgaa acagctcccg atgtttggtt attttaccta tcttaatgat
     5401 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
     5461 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
     5521 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
     5581 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
     5641 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
     5701 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
     5761 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
     5821 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
     5881 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
     5941 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
     6001 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
     6061 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
     6121 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
     6181 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
     6241 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
     6301 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
     6361 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
     6421 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
     6481 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
     6541 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
     6601 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
     6661 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
     6721 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
     6781 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
     6841 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
     6901 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
     6961 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
     7021 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
     7081 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
     7141 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
     7201 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
     7261 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
     7321 cagtcctcat tctatcacat cttcactttt ttgatgatgc aaat
//
LOCUS       DAHHPA010000115         7300 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.120, whole genome shotgun sequence.
ACCESSION   DAHHPA010000115 DAHHPA010000000
VERSION     DAHHPA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.120"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(59..1405)
                     /gene="gor"
                     /locus_tag="NBH55_002474"
     CDS             complement(59..1405)
                     /gene="gor"
                     /locus_tag="NBH55_002474"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCD1510414.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1539..2792)
                     /locus_tag="NBH55_002475"
     CDS             complement(1539..2792)
                     /locus_tag="NBH55_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD1510415.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(2798..3721)
                     /locus_tag="NBH55_002476"
     CDS             complement(2798..3721)
                     /locus_tag="NBH55_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD1510416.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(3942..6434)
                     /locus_tag="NBH55_002477"
     CDS             complement(3942..6434)
                     /locus_tag="NBH55_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCD1510417.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6441..6908)
                     /locus_tag="NBH55_002478"
     CDS             complement(6441..6908)
                     /locus_tag="NBH55_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCD1510418.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7044..7117)
                     /locus_tag="NBH55_002479"
     tRNA            complement(7044..7117)
                     /locus_tag="NBH55_002479"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7081..7083),aa:Arg,seq:acg)
     gene            complement(7208..7281)
                     /locus_tag="NBH55_002480"
     tRNA            complement(7208..7281)
                     /locus_tag="NBH55_002480"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7245..7247),aa:Pro,seq:tgg)
BASE COUNT         2023 a         1603 c         1184 g         2490 t
ORIGIN      
        1 attttctggt tttacatggt ttgttcaata aaaagctgtt ggaactgtta atattaaatt
       61 atcgcatcgt tacgaattct tccgctcctg ttggatggat ggctactgta ttgtcaaagt
      121 cttctttagt tgcacccatc ttgatagcca cggcaaaacc ttgcagcatc tcatcgacac
      181 caacaccaat agcatgtaag ccgacgattc tttcttcttt tccgacacag atcaatttca
      241 tttcgcattt ttggcgataa tcatttaatg caaagtacat tggggtaaag cgtgaacgat
      301 aaactttcaa ttcattttcg ccatattttt ccaatgcttc ttcttctgtc aaaccaattg
      361 ttgcaattgg tggatgcgta aagacgactg tcggaacaag tttatagtca agataagaat
      421 ctttctttcc attaaataaa cgttctgata aacgtctacc agctgcgatt gcaacaggag
      481 tcagatctaa tttaccgatc acatcaccaa cagcaaaaat atgttcatcc gttgtatttt
      541 ggtatttatc tactttgata aacccttttt tgtccaattc cacatttgta ttttccagac
      601 ctaatttttc ggtattcggt acacgtccac cggcaaaaag tacacaatca ccagttaagg
      661 tttcaccatt ttcaaatagc accgtatatt cgtcattgtt tttagtaatt tctttagcaa
      721 tatgatgtgc gtaagttttt attccagctt cttggtacat ttggatcaaa ttgtccgaaa
      781 gcattttatc aaacgttcta agtggtcgtt cttttcgata tgcccacatg acttcactac
      841 ctaaaccacg aaatacacct gaaagttcag ccgcaatgta tccagcacct aaaacgattg
      901 ctctcttagg taactctgtt aattcaaaaa atccatttga atcaattgcg tattctccac
      961 caggaatggt cattttactt gcacggcctc cagtagcgat caagatttta ggagcacgat
     1021 aggtttcacc gtttacttcg atcgtttgac tatcgataaa agtagcgtaa ccttttatag
     1081 cctcgacatg attgctatct aatcctcttt gataagcacc gtgtagaaaa tcaatatatt
     1141 tttctcgtct ttctactaga ccggcgaaat caacattttt taaatctgct tggataccgt
     1201 aacttgaggc atctcgttca attgtttcca aaatcgtact tgcttgccac ataacttttt
     1261 taggtacaca accgacgttg acacatgttc cgcccaattc attcgcttca atcagcaaaa
     1321 cttttgcgcc atgcatccct gctctgtttg ctgaagcaat accgccagag ccgccaccaa
     1381 ttacgatgta atcatatatt tgcattttgt tcctccttat tgaagagatc aatcaaaacc
     1441 tttatttaac gatccttttt ctacagaaaa caagtgtaac acttttcttg aattttgtat
     1501 gcgaagatgc ttaaaacagg gatatatcag ctttttcctt atttacgttt tcggatcata
     1561 tctccaggtt tcaccggctg tgcgactggc atagttaaaa tcatcattgg atttggtgct
     1621 cggtcaatgg attcgccttc ttcattatgc ataacttcaa cggtttgact gaaatgactg
     1681 aatcctggac cataaaattc gatttcatct cctacagaaa acaagtttct ctgacgtatc
     1741 gtagcaatct gtgttgcctc atcataagat aatacttcac cgacaaattt atatactggg
     1801 attttacgac gttcaccgaa taattgttcg ttttcagttg gtgtattgta gtaaaaacct
     1861 gtagctaatt ctcgttgtgc tactttccat aactcgtcga tccattcttg cttgcagaca
     1921 tagttttcag gatcttccat ataactgtct acagccgctt tatagacatt tgacaccgta
     1981 gatacataat ggatcgattt cattcttcct tcgatcttga agctgtctac gccgttttca
     2041 atcaattctg gaatatgttc gatcattgac atatccactg cactcatcga gaattcttct
     2101 tccacttcgc ctgtatcagt taaactgttt cgttcttgac caaaaggcat atcgaacaat
     2161 ccatacttcc agcgacaaga ttgagaacat ccgccacggt ttgcatcccg catcgacata
     2221 tggttggaca atgtgcatcg accagaataa gagatacaca tggcaccatg gataaatgct
     2281 tcgatttcgg tatccgtatt tttacgaatc tctgccactt cctccattga aacttcacga
     2341 gctaaaacga cacgttccaa cccttcttct ttccagaatt ccaatgtctc aaaattagtt
     2401 gctgaagctt gagtggataa atggatcggc agacctgggg cttctgtcgc acaaatttcg
     2461 atcaaagctg gatcagaaac aatcactgca gaaataccga catcacgcaa actgcggaaa
     2521 aattcccctg ctccatcttc atttccttca tgggtgacca tattagcagc aacgtatacc
     2581 ttcgcatcat gttcttttgc aaaagcaact ccttcagcca tttcttcata agtaaaatta
     2641 cctgcacggc ttcttaaacc atatgcattc ccgccaatat atactgcatc tgctccataa
     2701 tggatcgcgg tttttaactt ttccagtgtt cctgctggcg ctaggatttc tggtcgtttc
     2761 aattttcttt ttactgtcat ttctttcatc atactcctta tttgatagca gaaggatcaa
     2821 tataaaagaa tcctgtatct aattcacggt tttcaggatg caatttactg atttcgctag
     2881 tcattttttc cgctttttct tgatcccagc taccttctaa aatttgtttt tttgcttcgg
     2941 cgaatagtcg agtgatgttt acgaattttt cgcctggagt aaataatccg tccaatttcc
     3001 atgtacagta atgatgctga taaagttcat tcaattcatt cgagagattc agatcattgc
     3061 tggcaaaaat atgtgtgcca tgactatctt cataaataga ataatgtgtc tcttcttttt
     3121 taggttccga gataaaaaga cctctttcac gatctttttg ttcgtcttgt ttcgtatagt
     3181 tatagtaatt ctgtagcagt ggacgtttgg attgatggat gcatgtagca ccatacacaa
     3241 gggtttctac aggaatatcg agtttttctt ccatcgcctt catctcttca aaaggaactt
     3301 cacgagcaag tactgctcca actgcgcctt ttttcgccca gaaattgatt tgacgtgcgc
     3361 tcgttactaa tgtttcagca tcataaatat acggtatacg caattcagga ttttttttca
     3421 tcgtataaat aatacctgta tcaccaactg tgatcacatc tacgtttatt tcttttaaaa
     3481 aagccaaata ttcaggtaca tttttcattt tttcaggatg aatgatccca tttacagcaa
     3541 tattcgcttt tttaccatac ttgtgtgcta attctaccaa ttgtcgctgt tcttctcgtg
     3601 taaatgaagc aggcagacgc aatccaaatt gctcttcacc gaaatatagt gtatctgcac
     3661 ctgcagcaag caactgttct gcttgttcaa tagtttctac tgtggttatg agttcgatca
     3721 taattttctc ctcctacaac ttcataatag tagcatagct ctaaaaactt tccaatactt
     3781 cttctattta aagtcggatt tttgaaaatt atttcacaaa aaatcaaaat aaagttcgat
     3841 atattctgtc caaatataaa aagagattgt gacaaaaatc ttgtcacaac ctcttaatca
     3901 agtcttacta ttataaaaat tcagctacac aggccaagta actataggga acctactgtt
     3961 tcctttttag gtccgcgaac attcaatgtg atcttacctt ttgaagcacc tactgttacg
     4021 tggtcaccta aacgaatttc tccgccaagc aaagcctcgc tcaatcgatc ttcaatttct
     4081 ttttgaagtg cgcgacggat tggacgtgca ccatattcag gatcaaatcc agctttcccg
     4141 atcacatcga ttgcagatgg tgtgattttc acttgaatat cttgttcttt caatcgtttg
     4201 ataatcgctt tgctcatgat ttggacgatt gtatggattt catctttact caatgaatgg
     4261 aagacgacag tctcatcgat tcgattcaag aattcaggtc ggaaagcttt ctttaactct
     4321 tctaagatac gtttttgcat ggctttgtga tctttagtaa catcttggac attgaatccg
     4381 acattttttt cttctcgaat ttgtgtcgct ccgatatttg aagtcataat catgattgta
     4441 tttctaaagt ccacttttct acctttagaa tctgttaaat gaccatcatc caatacttgc
     4501 agaagtaaat tgaaaacgtc tggatgtgct ttctccactt catctaagag aatcactgaa
     4561 tatggttttt gacgaatttt ttctgtcaac tgtccacctt catcataccc tacgtatcct
     4621 ggaggagacc cgattaatcg gcttgtactg tatttttcca taaattctga catatcaaca
     4681 cggatcaggg catcttcact tccaaacatg acttctgaaa gtgctttggc caattctgtt
     4741 tttccgacac cagttggtcc aaggaacatg aatgaaccaa ttggacgatc aggatctttc
     4801 aatccgcttc gtgcacggcg gatcgcacgt gaaactgctt tgacagcctc atcttgacca
     4861 acgacacgtt catgcagaag tccttccagt tctaacaagc gttcgctttc ttttttctcc
     4921 aactgctgta gagggacacc agtccactcc gatacaactg tagcaacatc ttcttctgtt
     4981 acacgatcag aataaccttg ctcctgtttt gcttcaattg cagaaacttt ttgcagcttt
     5041 ttacgtagtg ctttttcttg tctgcgtaat tgagcagcat tttcaaagtc ttgtttttgg
     5101 atcgctgctt ccttttcttg tactagttca tcgatttcta gtttgatcac agttgatttt
     5161 gttaagtgat cggtttgatc caaacggact ttcgctgctg attcatcgat caaatcaatt
     5221 gctttatcgg gtagttgacg agaagtgata taacgaacag aaagattgac tgctgctcgt
     5281 acagcttcat ctgtgatctc cactccatgg tgctcttcat atcttgtacg taatccttga
     5341 agaatcactt ctgcttcttc aggagttggc tcatctactt gaatgcgagc aaaacgtcgc
     5401 tctaatgcag aatctttttc gatatatttt tgatattcat ctagggtcgt tgctccgatc
     5461 gtttgtaatt ctcctcgagc taacgccggt ttcaagatat tcgaagcatc aattgctcct
     5521 tctgctccac ctgcaccaat taaggtatgc aattcatcaa tgaataagat tacttgacca
     5581 tcattataga tttcgtcgat gacctttttc attcgatctt caaattcacc gcgatatttc
     5641 gtaccagcaa ccaaagatcc catatcaagc atcatcaggc gcttttcttg catgtcttct
     5701 ggcacttcac ctaaaatgat tttttgagct aacccttcag caatggctgt tttgcctaca
     5761 ccaggttcac ccaccagtac agggttattc tttgtccgtc ggctaagaat ctggataagc
     5821 cgtttcactt ctctcgaacg accaacaact gggtccagct tgttttcttt tgctaatttc
     5881 gtcaaatcac gtgctaatga atctagcgta ggtgttcctt gcggtgtttg ccgtttatct
     5941 tgtacattta catttctacg acgattcatt ccttttccag cagaatcacc agctagcccc
     6001 attttttgtt tcagaacttg acgggctttt gaaaggctga tacctaagtt gatcatgatt
     6061 cgagtagcca tgatactttc atcttttaat aagctcatca aaatatgttc tgttccaact
     6121 ttttgagaac cagttttctt cacttcgctg ctagctaaag caaaaacttg tttcattctt
     6181 ggtgaatatg gaagaaagac tcctttaggg taagaacgga taccaccata accaatcaga
     6241 tgttcaattt cttcataaat ttccgtttca ttcaaatcca tttcacgtaa tacttttcca
     6301 gcaattccat tttgctcgac cactaaggct agtaaaacat gttctgaact aactgattga
     6361 tgtttcaacc gttttgcttc ttcttgtgca atttctaata ctgcttgggc tctttcagta
     6421 aaaagttctt ccatctaatc tcacttccca tcttcataac ttaaacgttc cagaaatgct
     6481 cggagaatcc gagcacgtgt atgatcttca tattcattga aattcaatgt gttttttgca
     6541 attgcactca acatcaagtt tccttctttt tttgtaatta tttggtcttc ataaagtttt
     6601 tgaataatag caaaagcatt tttctcactg attgtatcat caaataactg atttatctgc
     6661 tcaagtaatt gttttttatc tgaaatcctt acttttgcaa ttcgtatata accaccacca
     6721 ccacgtttgc tttctactag ataaccacgt tggatggtaa aacgggtatt aatcacatag
     6781 ttgatttgtg aaggaacaca gttaaagagg tctgccattt caatccttcg aatctcgatc
     6841 atttcactat tttccagaat tttctttata tatgattcaa ttaaatccga ggtattctga
     6901 tgtgccatct tattcattcc ctttgactaa tattgacctt aattataacg aatttgataa
     6961 tagaaagaaa gaagaacgct ttttgttaaa taaatccttt agaatcaaca aaaaaaaata
     7021 cggaatatac tattccgtat cgacgcgccc aagaggagtc gaacccctaa ccttttgatc
     7081 cgtagtcaaa cactctatcc agttgagcta tgggcgcata ttcaatttaa aaaaattata
     7141 aatcatttga aaacaaaaag taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata
     7201 aaaaaaatcg ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct
     7261 accaagctga gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DAHHPA010000116         7185 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.121, whole genome shotgun sequence.
ACCESSION   DAHHPA010000116 DAHHPA010000000
VERSION     DAHHPA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.121"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..117
                     /locus_tag="NBH55_002481"
     CDS             <1..117
                     /locus_tag="NBH55_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCD1510419.1"
                     /translation="KNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSEILRR"
     gene            122..298
                     /locus_tag="NBH55_002482"
     CDS             122..298
                     /locus_tag="NBH55_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510420.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKRHERRQA"
     gene            359..676
                     /locus_tag="NBH55_002483"
     CDS             359..676
                     /locus_tag="NBH55_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510421.1"
                     /translation="MIIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            609..1088
                     /locus_tag="NBH55_002484"
     CDS             609..1088
                     /locus_tag="NBH55_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HCD1510422.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKEFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            1101..1811
                     /locus_tag="NBH55_002485"
     CDS             1101..1811
                     /locus_tag="NBH55_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010721532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1510423.1"
                     /translation="MEIKSATDILRDKDWRIMIYAKPGTGKTSLVQFLKGKTLVLDMD
                     DSSKVLAGLPNVDVISFDRVKPHEFITEFIKEAPELVTNYDNLVIDNISSFEKDWFVE
                     RGRSSKNGISNEIQDYSQWTNYFARIMTTIYLLKDINILVTAWENQREITTERGQVFN
                     QYAPQIRDSVRDNLMGLTDIVGRMIINPDTGNRGIILEGNDAVYAKNRLDERKATPAE
                     ELFFIGGEPNVSTPTVSD"
     gene            1783..3306
                     /locus_tag="NBH55_002486"
     CDS             1783..3306
                     /locus_tag="NBH55_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1510424.1"
                     /translation="MYQLRPYQIKLVQEARKHLSQGKKGVLIQSPPGSGKSVVIAEIV
                     RLATRKGGTVLFLAHRRELLDNIRETLEQNEVDLSKVIILSAVMAKNRLNKLPKLSLI
                     ITDEGHHGKAKTYMDVYNHFKEIPRLGFTATPYRLNGEGFTDIYEEMVEGPSIQWLID
                     HHNLAPYRWYSIPLIDRSKVDFKNMSREAESSARLFESDATIQGDIVENYKKYADGQQ
                     AIVYAPTIQVSKMIVRWFNDNGIYAVHADGKTPTKERDDIMANFKSKKITILSNVDLI
                     SEGFNVPDVGVIILCRPTQSIVLHLQQSMRGMRYRENKTSIVLDHVGNGANLGLPTDE
                     FEWSLSARKKKSNGSSSEAPRMTCSTCGQQFLLKSLLKIENKPHCPFCLQEIVIKEKE
                     NSVTFDEAVQMVELNAENAKLARLSRKKFSKKQSLELNYAIAKAKVSFEGKGNPLFKM
                     FGSLTAYQGQHYSIDQLEELSLIKDVSMESILRAYKWALEKLNSKQEEPEWVKNTFY"
     gene            3324..3830
                     /locus_tag="NBH55_002487"
     CDS             3324..3830
                     /locus_tag="NBH55_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HCD1510425.1"
                     /translation="MTAFKVDYNEAQDFGAVPDGDYEVVIFNVTEDASKGGTEFINFD
                     MVIRNDIKQPRQNSHLFHRVWKSKETGKYNRGMIMSLAKSFGLPDGKEYQSFENFLED
                     FAMRPAKVKVKNEQSEYNGKTYDNTNIKKFETTKFPELQHQWKKSNAEKSVNSSPAFD
                     ISDDELPF"
     gene            3830..6166
                     /locus_tag="NBH55_002488"
     CDS             3830..6166
                     /locus_tag="NBH55_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCD1510426.1"
                     /translation="MNNYERIPLELRELNQWGIYKRSWNEQRGKWSKKPYDPFTGELG
                     SSTNESKWSDFKTALSAVSTFNADGLAFYFKPPYIGIDLDDIGDDLERYLQGDVENNL
                     VYVFMNSTKTYSEISMSGKGIHIIGKAAIPGERRRKGNVEMYTEGRFFAITGNFFGNN
                     EEINEIPEIQMNFLYKRYLENETVIKQDFSKSNWSDGNDLSVNEIIQTAVNSSTGNRF
                     RLFMDGGWEKIYDSQSEADLAFANDLAFWTAGDFQKMDEIFRMSSLFRDKYDQKRGKT
                     TYGIGLLNKAISENTNHYTGKKTADDYFLSIPGITVDETKPTKFYSYDDTGNAERYLD
                     LFGSFTKYSYVNKCWYFYNGKNWEQDNIGAVRKWVDQTIEIFKNEPVSIPKDATEDEE
                     KAYIEAKEKHLRRSRNNAGKEAMTRELKHQVAILPDEFDSDDMLFNAQNGYLDLSNGI
                     LYEHDISKMFTRISNAEYTDKSDCPRWKLFLEQIFDNNTELIRYIQKAVGYSMSASIR
                     EQVMFILFGNGRNGKSVFLDIISEIMGSYAMGMQASSLMVKQGGSSGHNEDIARLNGA
                     RLVTSSEPNEGVRMDEGLIKQLTGGDKVSASYKGGHMFDYKPKYKIWLATNHKPIIRG
                     NDDGIWRRLPLIPFTVQIPLDKVDKNLKEKLMRELPGIFNWAVEGCLMWQREGLNPPA
                     DIQKATMEYRKEMDIIGAFIDECCETGPGYSIGATDLFKAYDKWARDMNEHPFSQTQF
                     GKKAADRFEKSKSKGKVVYRGIDLKKEFREFNVLVPGL"
     gene            6481..6798
                     /locus_tag="NBH55_002489"
     CDS             6481..6798
                     /locus_tag="NBH55_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCD1510427.1"
                     /translation="MTAEIEIQNAIRRELPKYGHFVYRGNVGKVKTIDGRWFDTGLPK
                     GWPDLFGWTKEGKFFAIEVKNEKGKLRPDQVKFGEFLQKQPVLYGVARSVEEALKIVE
                     ESS"
     gene            6795..6956
                     /locus_tag="NBH55_002490"
     CDS             6795..6956
                     /locus_tag="NBH55_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCD1510428.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            6953..>7185
                     /locus_tag="NBH55_002491"
     CDS             6953..>7185
                     /locus_tag="NBH55_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510429.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKTK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKN"
BASE COUNT         2638 a         1064 c         1529 g         1954 t
ORIGIN      
        1 aaaaacaaaa atggatttgt ctattatcca gttgaaggaa gagacacata tctattttta
       61 agaagtaaaa cacttgaatt tttagaaaca aatttttcgg aaatcttaag gaggtaagcg
      121 gatgggtaaa tttaatagag cactagtatt cagcgcacca ctaattatct acgctttagg
      181 tctttgggga agcagacaag cattaatcgg cacaattgta tatatggtct ggatctttat
      241 ggggcttgat gaagctgagt acagagcgaa aaaaagacac gaacgccggc aagcatgatc
      301 gtgtccaaag aaataagcta tctttctata ttttaccaca gaagggaatg aaccgtaaat
      361 gataattgca acggatacat tagatcgaat ctttttaaaa gacgaataca aactgcgcaa
      421 tatagatgcg tcgggaattt tagtatttga tctttatgac aatggaaaaa ttggcatcta
      481 tcaagcaagt gatattgaag aaacaaacct tgctttcgag caaattgatg attctgtgga
      541 attggattta gatgaagcaa tcctagcttt tgaacaaatt gcaaaattat taaaggaggc
      601 acaaaagaat ggcaactctt taccaactca gcgagtcata tatcaaagtc ctggaactgg
      661 cagaacaatt ggatgaagaa attcttcgcg atactctcga ttcgattaat gaagcgatcg
      721 agtataaggc agaaaactta gcaaaaatag ttaaagaagt agaagggaaa gctgagttaa
      781 tcgatagtga aattaaacgt ctacaggagc gtaagacatc gcttttgaac aatgctaaga
      841 gtatcaaaca ctatttacaa gaggagatgg aaaagactgg taaaacgaag attaaaggtg
      901 aattattcaa cattggaatt caaaataatc cagtatctgt caatgtaatc aatgaaaact
      961 taattccaaa agaatttttt acccctgtgc ctccaaaatt ggataaaaag caattgaagg
     1021 aggaactgaa gcacggagat attcctggtg ctgaactcgt ccaaacaaaa ggtttgagga
     1081 taagatagga ggcaatgttc gtggaaatta aaagcgcaac agatattctt cgtgataaag
     1141 actggagaat aatgatttat gcaaaaccag gtacaggtaa gacgtcactt gttcaatttt
     1201 taaaaggaaa aactttagtg ttggatatgg atgattcttc aaaagtatta gcaggtttgc
     1261 ctaatgtgga tgtgatctcg tttgatcgtg ttaaaccaca tgaattcatt actgaattta
     1321 ttaaagaggc tcctgaacta gttacaaatt atgacaatct tgttatcgat aatatttcaa
     1381 gttttgaaaa agattggttt gtagaacgtg gaagatcttc taaaaatgga attagtaatg
     1441 aaattcaaga ttactcacaa tggacgaact attttgctcg aattatgact accatttatc
     1501 tattaaaaga catcaatatt ttagtaactg catgggaaaa tcagcgtgaa ataactactg
     1561 aacgcggaca agtatttaat cagtacgcac cacaaatcag agatagtgtc agagataatc
     1621 ttatgggtct aactgatatc gttgggcgaa tgattatcaa tccagatact ggcaatcgag
     1681 ggattatctt agaaggtaat gatgctgttt atgctaagaa cagactagat gaacggaaag
     1741 ctacacctgc agaagaattg ttcttcatag gcggtgaacc gaatgtatca actccgaccg
     1801 tatcagatta agctagttca agaagccaga aaacatctat ctcaaggaaa aaagggagta
     1861 ttgatccaat cgccaccagg aagtggtaaa tcagttgtaa ttgcagaaat tgttcgattg
     1921 gcaacaagaa aaggtggcac agtactattt cttgcccaca ggagagagtt acttgataac
     1981 attcgagaaa ctcttgaaca aaatgaagta gatttatcaa aagtcataat attgtcagct
     2041 gtaatggcta agaacaggtt aaataaattg ccaaagctgt ctctgataat tacggatgaa
     2101 gggcatcacg gtaaggcgaa aacttatatg gatgtctata accattttaa agaaattcct
     2161 agattaggtt ttactgctac accatacaga ctcaacgggg aaggatttac agatatatac
     2221 gaagaaatgg tagaaggacc atccatacag tggttgattg atcatcacaa tctagcgcct
     2281 tatcgttggt attctattcc tttgattgat cgttctaaag tagattttaa aaatatgtca
     2341 cgtgaagctg aaagttctgc gcgattattc gagtcagatg ccacaattca aggcgacatt
     2401 gttgaaaatt ataaaaaata tgcggatggt caacaagcga ttgtttatgc tccaacgatt
     2461 caggtaagca agatgattgt tagatggttt aacgacaacg gaatatatgc ggttcatgca
     2521 gatgggaaaa cacctaccaa agaacgtgat gatattatgg caaattttaa atcgaaaaaa
     2581 atcactatat tatcaaatgt cgatttaatc agcgaaggat ttaatgtacc agatgttgga
     2641 gtgattatcc tttgtaggcc tacgcaatca atcgtcttac atttacaaca gtctatgcga
     2701 ggcatgagat atcgagaaaa taagacttcg attgtgttag atcatgttgg gaacggagct
     2761 aacttaggtt tacctactga tgaatttgaa tggtcgttaa gcgcgaggaa aaagaagagt
     2821 aatggaagta gcagcgaagc gcctagaatg acttgctcaa catgtggaca gcagtttctt
     2881 ctgaagagct tattaaagat agaaaataaa ccacattgcc cgttctgctt acaagaaata
     2941 gtaattaaag aaaaagaaaa ttccgttact tttgatgaag cggttcaaat ggtggaattg
     3001 aatgcagaaa atgccaagct agcgcgactt tcaagaaaga aattttcaaa aaaacaatct
     3061 ttagaattaa attatgcgat tgcaaaagca aaggtaagtt ttgaaggaaa aggaaatccg
     3121 ctttttaaaa tgtttggctc actcactgct tatcaaggac aacattactc catcgatcaa
     3181 ttagaagaat tatcgttgat caaagatgta tcaatggaat caattttaag agcttataaa
     3241 tgggctttgg aaaaactcaa ttcaaaacaa gaagaacccg aatgggtaaa aaatacattt
     3301 tattaaggaa gaggtaatta attatgacag catttaaagt agattacaat gaagcgcaag
     3361 attttggagc agtaccagac ggagattatg aggtagttat tttcaacgtt acggaagatg
     3421 cctcaaaagg cggcacagag tttattaatt ttgatatggt catcagaaat gatattaaac
     3481 aaccacgaca aaacagtcat cttttccata gagtttggaa aagtaaagaa actggcaaat
     3541 ataatcgtgg gatgatcatg tcactagcaa aatcatttgg ccttcctgat ggaaaggaat
     3601 atcaatcatt cgaaaatttc cttgaagatt ttgctatgag accagcgaaa gtaaaagtaa
     3661 agaacgaaca atcggagtac aacgggaaaa cgtatgacaa tacaaatatt aaaaaattcg
     3721 aaactactaa gttcccagaa ctccaacatc aatggaagaa aagcaacgct gaaaaatctg
     3781 taaattcctc accagcattt gatatttcag atgatgaact tccattctaa tgaataatta
     3841 cgagcgtatt cccttagagt tgcgtgagtt aaatcagtgg gggatttata aacgctcatg
     3901 gaacgaacaa cgagggaaat ggagtaagaa accttatgat ccgtttactg gagaattagg
     3961 cagcagtaca aacgagagca agtggtctga tttcaagacc gctctctcag ctgtttcaac
     4021 ttttaatgca gatggcctag ctttttattt taaaccacct tatataggga ttgatttaga
     4081 tgatataggt gatgatttag aaaggtatct tcaaggcgat gtagaaaata atttagttta
     4141 cgtttttatg aattctacaa aaacatattc agaaatatca atgagtggca agggaatcca
     4201 tattataggt aaagcagcaa tccctggaga aagacgtaga aaagggaatg tcgaaatgta
     4261 taccgaaggt aggttctttg ctattacagg taacttcttt ggtaataatg aagaaattaa
     4321 tgagatacct gaaattcaaa tgaatttctt atacaagcga tatttagaaa atgaaacagt
     4381 aataaaacaa gatttttcta aaagtaattg gtcagatgga aatgacttat ctgtcaacga
     4441 gattatacaa acagcggtga attcttccac aggtaatcgt tttagattat tcatggatgg
     4501 aggatgggaa aaaatatatg atagccaatc tgaagcagat ttagcatttg caaatgattt
     4561 agctttttgg actgctggag attttcaaaa gatggatgaa atattccgaa tgtcttcgtt
     4621 atttcgagac aaatatgatc agaaacgcgg aaagacaacc tatggaatag ggcttttaaa
     4681 taaagccata tctgagaata caaatcatta tactggcaaa aaaacagctg atgattattt
     4741 tctttccatc ccaggtatca ctgtggacga aactaaaccg actaagtttt atagctatga
     4801 cgatacagga aatgcagaaa gatatcttga tttgtttgga tcatttacaa aatacagtta
     4861 cgtaaataaa tgctggtatt tttataacgg taaaaactgg gaacaagaca atatcggtgc
     4921 cgttagaaaa tgggtagatc agactatcga gatatttaaa aacgaacctg tttcgatacc
     4981 taaagatgcg actgaagatg aagaaaaagc ctatattgaa gcaaaagaga aacatttaag
     5041 aagatctaga aacaatgctg gaaaagaagc tatgacacgt gagttgaaac atcaagtagc
     5101 gatacttcct gatgaatttg atagtgatga tatgctgttc aacgctcaaa acggttattt
     5161 agatctttct aatggcattt tatacgaaca cgatatttct aaaatgttca ctcgaatttc
     5221 taacgctgag tatacggata aaagtgattg tccacgttgg aagctgtttt tggaacagat
     5281 atttgacaat aacactgaat tgattcgcta tattcaaaaa gccgttgggt attctatgag
     5341 cgcatcaata agagaacaag tcatgttcat ccttttcggt aatggaagaa atggtaagtc
     5401 tgttttctta gatatcattt ctgaaataat gggaagttat gccatgggaa tgcaggcatc
     5461 aagcttgatg gttaaacaag gtggtagcag cggtcataac gaagatattg cacgattaaa
     5521 tggcgcacgt ctagtaacgt cctcggagcc aaatgaaggc gtaagaatgg atgaaggttt
     5581 aatcaaacaa ctaactggtg gagataaagt gtcagcatcc tataaaggag gccacatgtt
     5641 tgactataaa cctaaatata agatttggct tgcaactaat cataagccaa tcatcagagg
     5701 aaatgatgat ggtatttggc gaagattgcc attaattcct ttcactgtac aaattccatt
     5761 ggataaagtg gataagaatt tgaaagaaaa attgatgcgc gaattgccag gaatattcaa
     5821 ttgggcagta gaaggatgtt tgatgtggca aagagaaggg ttgaatccac cggccgatat
     5881 tcagaaagct acaatggaat atcgaaaaga aatggatatt ataggtgctt ttatagatga
     5941 atgttgcgaa acaggacctg gttattcaat cggggcaacg gatctattta aagcatacga
     6001 taaatgggca agagatatga atgaacatcc attcagtcag acccagtttg gtaagaaagc
     6061 tgcggacagg tttgagaaat caaaatcaaa agggaaagtt gtttatcggg ggattgattt
     6121 aaaaaaagag tttagagaat ttaatgtatt agttcctggg ttgtgaaaca aaaaagtttc
     6181 acaaatgtgt gtaggtagac agttgggtag atagtaagta gatagttttt tacaaactgt
     6241 ctacccgata aaacctttgg ggctgtaagg ctcattactg ttaggtagat agtagggata
     6301 gtttatatat atagtaaata aaataataaa aataaggaaa tatataaaaa tacagaagca
     6361 acctagaaat aactgtctac tgtctaccca aactggctaa tacttagagc cacaagggat
     6421 agagcgatta taaactgtct acccatgtat atatactatc taccccagca ggaggcgctt
     6481 atgacagcag aaattgaaat acaaaatgct attcgaagag aattaccaaa atatggtcat
     6541 tttgtttata gaggcaatgt gggcaaagtg aagacgattg atggcagatg gttcgatact
     6601 ggattgccca aaggatggcc agatttattt ggatggacta aagagggaaa atttttcgcc
     6661 attgaagtga agaacgaaaa aggaaagttg agaccagacc aagtgaagtt tggtgaattt
     6721 ttgcaaaagc aacctgtact atatggtgtt gcacgatcag tggaagaagc attgaaaatt
     6781 gtggaggaat catcatgaca acagaagaag tgattcaaat gcgaattcga agcattcaac
     6841 gtgagattga cgatctggaa cgaacaaagg cagtgatggt caatgaaacg gcgagaaagg
     6901 caatcgattt gcacatagag aacttaagaa gggaaattcg tagattggag gaatgagcgt
     6961 ggataagaaa gcagcaatga aacgaatcat cgaactgaca cattctgaga attggcaaga
     7021 agacaaagaa atagttgcag aagttcaaaa gctcggtaaa tcaatgtggg ctgaaaaaac
     7081 caaacggaaa acgccgagaa aaattgcaat ctggcatggt gatcgaattc tagtaacagg
     7141 taccgctgaa cagttatctg aaattactgg tctgagcaaa aacat
//
LOCUS       DAHHPA010000117         6891 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.122, whole genome shotgun sequence.
ACCESSION   DAHHPA010000117 DAHHPA010000000
VERSION     DAHHPA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.122"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..525)
                     /locus_tag="NBH55_002492"
     CDS             complement(<1..525)
                     /locus_tag="NBH55_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD1510430.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLS"
     gene            complement(522..941)
                     /locus_tag="NBH55_002493"
     CDS             complement(522..941)
                     /locus_tag="NBH55_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCD1510431.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1011..1151)
                     /locus_tag="NBH55_002494"
     CDS             complement(1011..1151)
                     /locus_tag="NBH55_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCD1510432.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(1144..1491)
                     /locus_tag="NBH55_002495"
     CDS             complement(1144..1491)
                     /locus_tag="NBH55_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510433.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            complement(1505..1999)
                     /locus_tag="NBH55_002496"
     CDS             complement(1505..1999)
                     /locus_tag="NBH55_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510434.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(1999..2448)
                     /locus_tag="NBH55_002497"
     CDS             complement(1999..2448)
                     /locus_tag="NBH55_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510435.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(2452..3072)
                     /locus_tag="NBH55_002498"
     CDS             complement(2452..3072)
                     /locus_tag="NBH55_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510436.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(3072..3980)
                     /locus_tag="NBH55_002499"
     CDS             complement(3072..3980)
                     /locus_tag="NBH55_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCD1510437.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(3993..6755)
                     /locus_tag="NBH55_002500"
     CDS             complement(3993..6755)
                     /locus_tag="NBH55_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1510438.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            complement(6752..>6891)
                     /locus_tag="NBH55_002501"
     CDS             complement(6752..>6891)
                     /locus_tag="NBH55_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1510439.1"
                     /translation="DGNLEHQDQVTLFLDFPFLYPGKNEIKWTKNIHSFEIMPRWWRK
                     V"
BASE COUNT         1964 a         1486 c          992 g         2449 t
ORIGIN      
        1 tgataagtat ccatacggat cagtgtgatc gccccaaatg ttttgagtta cccataaatg
       61 tgatttgatt cctggttgat tatacggtgt gtctaatgta agtggaatac cgtatttatt
      121 agcggaatct ctcgctaatt caacatatgc cttgtagttc ttttcaaacg ttactttatc
      181 gtgcgtatgt tgcagttcga tttgcacagg actatttgca ttagcatatg aaccagcgcc
      241 atattgtaca taaccaggtt gtccaacttg gtaaacgatc ccaccatcgc caacaatata
      301 cgctgtatag gcattgtacc aagaacgttt catgtactgc gcttcatttc gtcctgtcgc
      361 agtttcgttt gctgtttcat gcaataggat gtattggtta tttgctactt gagaactacc
      421 ttcgtttgcg cccaaattga attcattgtt gatcgagtaa gcaaaggcgt ttattggcaa
      481 taaaaaaaga gctataatag ctcctacaaa gattttcttt ttcatttggt tcctcctcgg
      541 tctttatttt cagtatcatt tcgtatttgt tggaatacct cttttaactt atctggcaaa
      601 ggaaggaata ccgaaatgtt ttctaaaaaa ctaatccctt catttgcaac atagaacata
      661 ataactattt ctcttagagg tatagaattc ccaacgattt tctgtacgtc aaccgcaacg
      721 gcaatcacaa cgaagattaa gactttcttc aacaatcctt cgaaacctat ctcactactt
      781 atttctttca atttccatgc cttagcaagt ccagttaaga agtccacacc cacaagaaaa
      841 attaaaacat gtagtatatt gtccatccct cccaataaac caacaattgt cccgcctatc
      901 acccctgtca ttactgagag tgtatttaaa tatttttcca atatctattt ctcctttatg
      961 caaagaagcg cactcgtttg agtacgcttt ttatttaact ttgtgcttaa ttaaatttca
     1021 tttccagtaa tctgcttata ttggtctttt gttaaacagt tcatgtttac atagtcaagt
     1081 aaatcttggt ccgtataaca tccccaatca taaaattgtt ttatattttc aaagcccgga
     1141 aaactattca ttacttttcc ctccattctt aatacttgcg acttccatcg tcaattgagc
     1201 taacatttgt tgcatttttt cttgttcctg taccgatatc gccaattgtt ttgtcagtag
     1261 agcatttaat tgcttcaact gatcgacatc ttcaatatcg tcaaccgctg tgccactctt
     1321 ctcttcttca gtagctaact caacccactg ttttttatca aaatcaaatc ttggtctcca
     1381 gttaggaact ggtggtttaa tctctgtaca gttttcaggg atattttctt gattgttcaa
     1441 aatgatttgc tcgaatccgt aaggcttgat tgatttgtaa actactttca ttagatttcc
     1501 tcccttaaat tgaataagtg ataacaaatg aataagctga tccataactt gaatttcttc
     1561 tccatttaat ggctccgtct gcgccaatag atagttgagc actgttcaaa gtagaacggt
     1621 ctattgaccc aaccagttgt tcaaaactaa ttggtgtccg atagccttct ggaacagtta
     1681 aaataaccga atcattcccg ccactacttt ttccgtttaa agccacaaaa tatatagaaa
     1741 ctgtttttcc ttcacgataa agctttgctg tccctgtatt accgttagtg acaaccagat
     1801 ttaccacttc atatctgtta tcatcagtcg tcaaaacacg tttattattg attgttacag
     1861 aacctgaaaa attcttatgt cctgcaatgg cttggtctcc atctgtctgc accaatgctt
     1921 ctttaccatc aatagctttt acatgcgttt ttaagtattt agcaactccg tcttctttta
     1981 attgaacaat atcagccatt aaaccgtccc cactttctca aatgtaatat tggctaatcc
     2041 atcaagtttc actttatcgg ttgctgacat taaacctgct gtcgtagttg tagcattacc
     2101 tggatttttc tgtgctccag ctgcaattcc atccaactta gttttatctg tggacgacat
     2161 caacccattt gctgtagttg tagctacagc tgtagttgtg gcatttattc caggatcacc
     2221 tttatctccc tttggtaaaa caaaattaaa tctagctgca gatgatgttc ctacattcgt
     2281 aacagaagcg gttgaaccac tagaaacggt tcctatggta attgttgctg cttggccagg
     2341 atcgccttta tctcccttca ccgttgttgg tttgccttct atagcattcc aatgagtttg
     2401 tggataaacc tgtacattgc tttgttttat ttttacgata tctgtcattc tctatacctc
     2461 cccaattttt tcaaaagtaa aatttggaat tctttcgttt gtgtaatttt ctgcttgatt
     2521 tacagcttct tggaattttt gatctacata tgactgattg acaccaccag tcccactacc
     2581 acctgtagaa ctaattgttc catcttcatc aatagtgatg ttttctccag cagtataatt
     2641 tttcagtgaa tcgagttttt cttttaactc cagcgaaaaa ttgacatcag attgtctgac
     2701 tgcagagact ttccctgtct catcaatcgt caataattca ccaactttta tgccacccaa
     2761 acggtcgata gtggcaactg gcaacacgta gtcaccttct ccactgttta aaattttttg
     2821 atacatttct gccgtgataa tgccgtcatc ttcttcactt gcatagggta attctgtgag
     2881 aacattctct aatcccaaat cttctttagt aatgacgacg gctccaattt ttccattaac
     2941 agataaaact gttgcttctc ctgctattat ttcttctaac ccaagaactg cggaagcatg
     3001 tgtgattgga aagaattggc gttgtacacc tgttttttca tccgtttcca tcattcgttt
     3061 agttttagcc attagatcac cccgactttc tcaagagtga acacattttg ctttggatca
     3121 tcaacggttg cgatgatcaa ggctccttct tcaatcggga attctacagt tccaactttt
     3181 tcgacttcgt gattatctga aaagagatcg tcttgaagaa tatcagtcac ttcaatttca
     3241 ccgtattcaa tcgtgaaaag tgctgctttc attttttgat acaaataatt tagatcggat
     3301 aggaaacgtt cagaaatcga ttcatggcgt acaccttgaa gatctacacg tgcatccatt
     3361 aattcggcta acattactcc acctggatcg atagacttca aaatatcctt gattgattca
     3421 aaccatgaca aataatctga ttcttgtcct tctctccaat cttggaaact attctcctgt
     3481 tcttctctcc agcgatcaaa ttcttctttc ctttcattca tccagtccgt aaaatcgcct
     3541 ttattttcat taataaaatc tgtcatatca gcaattaaat cttcaattga ttgccaataa
     3601 gaacccattt ctccttctgt ttttgaaaca gcattgatga caaaatagga gaagtcttga
     3661 gttgttccaa tcaagtcttc tcctttaaat ataatgaaat tggctgtttg tcgatgcaaa
     3721 cactgcatgg aatatttatc aaaaatatat ttgattttcc cttttttagc atccacaatt
     3781 cttgtttcta attggactgg atattttccg ccaacaactg attcaaaata taccttacat
     3841 tctgataaat catatggaag accattttcg acaattgtag cttccataac ttcagtgttt
     3901 ttattgcctt gtcgaacttg aatcatcccc acgtaattat agggttctgt tgtacttaat
     3961 atgacattcc actttgccat taaatcacct ccctattttg gtggtatgac aatggaagaa
     4021 attgcacttg cactataata ctggcgatct aactttccac aaatcatacc taactctgtg
     4081 ttttgttcat aagtttgcat acgaccatta gctaagcctc ggataacgcc agtatgtcca
     4141 taagttccgt ctgcaaacca tgaacccact tgtccacctc ttgcccaatt aataattgca
     4201 ccaactacta attgatcata tctagggttt tgaatcactt tccaaccaac agcagaccaa
     4261 tcataggcaa taccaatatc agatgctgca gaagtatttc ctatcacatg agttaaacca
     4321 tattttgttc cagcacccat gccacaacca cccagatatc ctgaatattc tgcagacaag
     4381 ccataacact gaccattgcc aattcgttgt ccaatcaaag actctaaatg ttttagtcct
     4441 gcttctccag tagctcctcc tggttttaaa tctttaaatt tgttatacca gttaactgca
     4501 taatcttgac gttcaggatg tgtcgctgcc ggacgttcat agtttcgttc aaaagcatat
     4561 gctgcttgtc ttggatcagt acaggcctta aatccatcaa ccgtcgtagg ttgtactacg
     4621 cccatccatt gtccatttgt aaatgtccaa ataagcaatc gaacttgtgc atctaagctc
     4681 gttattggtt ctttaatacc tgcagcatta aataagtttt gaacataaac ttttccatcc
     4741 catgttgctg gaccaacaag aggatatgaa gaaccatccc actgaactaa tccgtatgct
     4801 ggtccaccta tttgaacagt atcaggatca aaagttcctc ctgtttcttg ttgaatgttc
     4861 cccaatattc cacatgcaga ttgtttcgta aatccgttat tacacaaaat atcgtaaatt
     4921 ctccaagctc tcttttctgc atctgttttt aattcactag gatacccacc agtagattct
     4981 ccacctcccg atggaccacc accttgtcca ggaattattt cttttccttt aactgtaagt
     5041 tttccttgta catctaagtc tccaaaataa attgctttac catttcctaa taaaactaat
     5101 ccttttccta ctttaggaga aatcaaaata tatttgccgt ctccatttgt acgaataact
     5161 aaagaattat cttcaatagg ggttggagta gaagccccag gaaaaggatt accagcagaa
     5221 tcagttgttc caatcgttcc aattgaaccc ttagaattcc aaaattccat tcctttttta
     5281 gttaactcca ttattttctt tttgttgttc caaatttgga gtagtccatt aactaatttc
     5341 aatacatctc cagtcttgtt aaaagaattt tgaaagatat ctgctttgat taatccagtt
     5401 tgtataaaat tggcattaaa tatactatct aatgtccaag ctgaagtaaa aggcccccgc
     5461 catcctttct tagaaaaagc aatcccattt ttattcattc ttagtacttc tcttgccttt
     5521 tctaaatctg gattatccat aataaagatg ttggaaggtt tttcttttgg ccataaactt
     5581 acatatcctc ctgcactgcc ttgacctgta atcattgaag aaacataatc attaaaatca
     5641 ctcatataat tattcgttgc ataatctttt aatttatcct gaatagttac agcttgttgc
     5701 tgataaaaag caacttgaat gtctcctgct tctaatttca aagttttttc agataaagaa
     5761 tcgtattgta gtccactaac ctttgattca agataaatat tatatttttt atgatatatt
     5821 ttaaatgtat caaacagacc ataatttcta atcttcgcaa attctttcgc ttcttcacta
     5881 tcagtcaact tatcaaattc aaccgtaata gaaactttag gcttatcaca gccaggattg
     5941 atcgttttaa agtaattttt agctatttta tttaagctct taatgtcttt tactccttgt
     6001 tcttctgtaa actgaacatg ctctgtatac acatcaggat agttattaat ataggcacta
     6061 tctactggcg aaccataaat tcgattagtc gtacctacct cactttgagg atcagcatat
     6121 ggaataagtc ttgtttttat acctgtccaa tctaatttga cctttaaacc agacatatct
     6181 tttccatacc gaatcgttcc aacattattg cgacctcttc gctttaacaa agaaagctta
     6241 aatggttcac gtttaatttc tccaccccaa tattgaagca atgatccttg ttcaccagca
     6301 atacaattta aaacatttct cgcttcaaag attgtgctag aaacggccgt aatatcagaa
     6361 taaagtcgta tgtcagattt tttatccatt ttggtttcaa taattgatat cgcttcttga
     6421 ccagttttag agtctacttc cgcaagagtg actacccgtc ttccaagtcg atttgtacga
     6481 ctctgagcat aaatagttac tgtatttaaa aaagtatcta tatctttatc atcaataaaa
     6541 aagatatgat actcttcttg atcgtttggc tttgctttta tttgatagtc attttcaaaa
     6601 tattcatcaa atctagttcc taaaggataa tccaactcta attcatattt tccattagct
     6661 acttcatata tttcacatct tgtacaatcc tttaaaattc ctaagccatt cgttgagaaa
     6721 tctgtttcag taggactata tattcttggt ttcatacttt tctccaccac ctaggcatta
     6781 tttcaaatga atgaatattt ttcgtccatt taatttcatt ttttccagga tataaaaatg
     6841 gaaaatctaa aaataaagtg acttgatcct gatgttctaa attaccatct a
//
LOCUS       DAHHPA010000118         6788 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.123, whole genome shotgun sequence.
ACCESSION   DAHHPA010000118 DAHHPA010000000
VERSION     DAHHPA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.123"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(718..3867)
                     /locus_tag="NBH55_002502"
     CDS             complement(718..3867)
                     /locus_tag="NBH55_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCD1510440.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3880..5079)
                     /locus_tag="NBH55_002503"
     CDS             complement(3880..5079)
                     /locus_tag="NBH55_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD1510441.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5069..6664)
                     /locus_tag="NBH55_002504"
     CDS             complement(5069..6664)
                     /locus_tag="NBH55_002504"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCD1510442.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1906 a         1398 c         1103 g         2381 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt
     2041 taaccaatca ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt
     2161 tgtataggca ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat
     2281 acgattgatc tgttctgatg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 gacagagcca tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtt gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc
     2701 atctagtttt tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt
     2761 ttcacttaat tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata
     3001 ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgagggttt tattatcttg atcttcactg acgattgtat aatccgcaat
     3121 caaacgatga gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac
     3181 tttttcattg tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg
     3361 ataaacacca tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag
     3541 gataagtgag acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc
     3601 attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc
     3661 cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagata attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg
     3781 acgatacgtc cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc
     3841 tgcttcctca cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa
     3901 gaagcttttt ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc
     3961 atctagctgt ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac
     4021 actgatattc tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct
     4081 tggcaaattt atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa
     4141 taaataagcc atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt
     4201 aagcattatt gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt
     4261 ccatttttta cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata
     4321 ctcattacca atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc
     4381 ttgctcggca tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg
     4441 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc
     4501 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgttg
     4561 atgaagagcg atagtgtcgt cgagtttttt gaagaatatg ccgatttttt gttgttcttt
     4621 taaatcattg ggaatataga atactgtatc tgaaactttt ttccagtctg aacgaggcat
     4681 ttttgttcca gatgtatcat ttgcagcctt ttgatatcta tcagcttgaa taagagaata
     4741 aataaagtcc gagtcgctct tcttagctct aaaaacccag aaatccccaa tcgctacgcc
     4801 tgtaaaagtt gccttgagcc aattttttaa atatggacgt aactttccat atagaatatc
     4861 atttggtaaa aattcaatcc cttttctatt atcaaatttc tggctcacat ctttattcag
     4921 tctaccttct cctgccacga tatcttcaaa ttcaactttt ggaagtctac tggagtttgt
     4981 acttttatta agtctaacaa ctaaatcaat taacttacgc tcttcccaat cttccgtaaa
     5041 tcctgggaaa cggatttctg gtcgtgtatc attgctcatg tttgaacacc tccagcgcac
     5101 cttgtaacca catttcgttt tcaggtgttg tttgcaggga agagatagct tctaaaagtt
     5161 ctttttctag cacttgctgt tcttgtcgta tcttcttgat gtcatttccg acatgaacca
     5221 tatcaattgg ttcttcttct tcgaaagtat ctacatagcg tgggatattt aagttatagt
     5281 cattttcttt gatttcatca aaggttgcca aatgagcgta tttctcgaca tcttttcttt
     5341 cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga tagtttattt tgatttttcc
     5401 ctttaacaaa ttcacgacta gcatcaatga ataatacatc tcgagtttga cgattcttct
     5461 tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa caagttcgca ggcataccga
     5521 tcacagcata aatactgcca tcttctagta atttttgacg gatgacccct tctgcagcgc
     5581 cacggaataa aactccgtgt ggtaagacga tggccatggt acctgtttcc ttcaaatgat
     5641 agaacccatg aagaagaaac gcaaaatctg ctttggattt tggcgctaat ttcccataac
     5701 gattaaaccg agaatcatct aagaaagtcg tatctgctga ccaattagcc gaataaggtg
     5761 ggttcattac tactgcatca aatgtgtaag gttcatctgt tggccagtcc ttattcaacg
     5821 tatccccatt gcggagattc atttcttcgg cgtcgacacc atgtaaaatc aagttcattt
     5881 ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg atatttaacg ttatctgggt
     5941 gtgttaagta gttacgtacg tttaacatca atgaacctga acccatcgtt ggatcaaaaa
     6001 cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat ttgcgccatc atatcagaaa
     6061 ccatatgagg tgtgtaaaat tctccggctt tcttccctgc ttctgaagca aattgactga
     6121 tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg ttctaaaaca tctacatcat
     6181 tcaatttctt gatgacttcg gtaatcgtca catttcgttg ttgctcatct gttcctagtt
     6241 tttttgattg gagatccaca tcatcaaata acccattaaa ttgattgtag gtagatgcca
     6301 attgcacaaa ggctttgttt agatcgttca attggaacgt tgcttgtttt gcttgatccg
     6361 ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc caaaatatca acgattgtgg
     6421 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac attgtttgtt
     6481 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
     6541 atcgatcgga taaatattta tagaaaatca gtcccaataa ataattttta tattctgacg
     6601 catccatttt actacggaga ttatctgccg cactaaataa tttttgattt aattctgcac
     6661 ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt attttagcat
     6721 accccctttt ataaaaagca aaaaaaaaag aaagaaactt attcttgcta ataaactacg
     6781 cgaaagga
//
LOCUS       DAHHPA010000119         6634 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.124, whole genome shotgun sequence.
ACCESSION   DAHHPA010000119 DAHHPA010000000
VERSION     DAHHPA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.124"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            46..1005
                     /locus_tag="NBH55_002505"
     CDS             46..1005
                     /locus_tag="NBH55_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD1510443.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            1247..1708
                     /locus_tag="NBH55_002506"
     CDS             1247..1708
                     /locus_tag="NBH55_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCD1510444.1"
                     /translation="MLDLAILLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVIT
                     KEDFANVPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVI
                     EGAVSHLSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1970..2785
                     /locus_tag="NBH55_002507"
     CDS             1970..2785
                     /locus_tag="NBH55_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCD1510445.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2795..3457
                     /locus_tag="NBH55_002508"
     CDS             2795..3457
                     /locus_tag="NBH55_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCD1510446.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3662..5326
                     /gene="alsS"
                     /locus_tag="NBH55_002509"
     CDS             3662..5326
                     /gene="alsS"
                     /locus_tag="NBH55_002509"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCD1510447.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            5339..6049
                     /gene="budA"
                     /locus_tag="NBH55_002510"
     CDS             5339..6049
                     /gene="budA"
                     /locus_tag="NBH55_002510"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCD1510448.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            6179..>6634
                     /locus_tag="NBH55_002511"
     CDS             6179..>6634
                     /locus_tag="NBH55_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCD1510449.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLL"
BASE COUNT         2163 a         1075 c         1390 g         2006 t
ORIGIN      
        1 cactcaaaat agttccgacg aaagaattat aaggagtgag tacaaatgac ctataaccat
       61 cttacaccaa ctgaactcgt aatgatagaa gcttatttca atcaatcaca acctgtttca
      121 aaagtagcca atctgttaca acgttctcga caaactattt ataaggtcta tcgttttctt
      181 aaatctggtg gaagcgcaat gagctactat aaacaatata agaaaaacaa acgtaactgc
      241 ggaagacatc cgattgtttt acctgatgag caacaggaat acattcaaaa aaaggttgtt
      301 caagggtggg ctcctgatgt ccttattggt cgagcagagt ttcctattgc ttgttcggta
      361 cgcacactgt atcgaatgtt taaagataaa acctttgaca ctcacgattt accaatgaag
      421 ggaaaaagaa aaccaaacgg tcataaagaa aaaagaggaa aacaagggtt tcgccggtca
      481 atccgagaca gaaagacaga ctatacccag tttgatcatg aatttggtca cctggaaggt
      541 gacactatcg tgggtggaaa acataaaagc gcggtcatca cattagttga acggctatct
      601 aaagtcatca ttaccttgaa acccgaaggc agacaggcta aagacattga aaataggctg
      661 aatcaatggt ttcgttgtgt cccaaaaaac ttatttaaat ccatcacatt tgattgtgga
      721 aaagaattct caaattggaa aaagattagt aataccaacg atatttcaat ttattttgcg
      781 gatccaggaa caccttctca acgaggattg aatgaacatt caaatggctt attacgtaaa
      841 gatggtttgt ataaagctat ggattttaat ggtgtatctg aaacctttat ccaatcggtg
      901 gcatcgaaac gaaatcatat tcctagaaaa tcattacact atcggacacc aatggaagta
      961 tttttgagtt acgtcacaaa agatgatttg tctaacttaa tttgacaaac gaaactaaaa
     1021 aagccgagca gaagaaattt tagatttaac gaatcaaacc actcaagaat tcgagaacag
     1081 aaaaaaacaa ttattcagcg gccgatttac gatcggctgt gtggaagctg ataattcgaa
     1141 tacattggca ctattactag aaaagttgat ccgtgattat cccagcgttc aatttcatat
     1201 ccacagcgga acaagtgatg atatcacgga gtgactagaa aaaagcctgt tagatttagc
     1261 gattttgcta gatccagtag atacagagaa atacgaaacg attttgttac caagaaagga
     1321 aaaatgggga ttgctcattt tagaaaattc tgttttagca aaaaaagacg tgataacaaa
     1381 agaagacttt gcgaatgtac ctcttttgat ctcaaagcgt ccagaaatac agcgaatgtt
     1441 tgctgactgg ttaggcataa aaaaggagca gttgctgatc acaggaacgt acaatttgat
     1501 tttcaatgta tttgcgctaa ttgagaatca ggtaggtgct gctttagtca tagaaggtgc
     1561 tgtaagtcat ctttcaaagc gagtgacatt cgttccgctt gacccaatga ttgaaacgaa
     1621 ctgcatgcta gtctggaaaa agaatcggat tttatcacct gtagtcaatg aattgattca
     1681 ccgtttcaaa gaagcttttg aacagtaata agtatctcta atcgaagaaa tttcaataca
     1741 aaagcgagac gtatgacagt cttgcttttg tattttttca tttttaaatc aaaaaaaata
     1801 aagagattta cataacttgt taaataagcg cttacaaaca taagaagaac ggaaatgaat
     1861 cagctataat aaccgttgtt ctggattgta atcattacaa agaaatgaag attataactt
     1921 tggaggtgtt cgaaatgaaa gaaaaaaagc aaaaattcgt tcaaaaaaca tgccctttat
     1981 tcaatggttg ggtatgattc tgctgattgt attagcttta ttgacgatca ttcctttttt
     2041 atggatggtt tctgcttcat tcaaagcaaa taatgaagtg ttcacgattc cgattcaatg
     2101 gatccctaaa acttggcatt tagagaatta tctggttatt tggtctcgta ttcctttagt
     2161 gacatttttt aagaatacag cttttttaag cgttgtgatc acgttgattc agttgtttac
     2221 ttctagtttt gctgcttacg ggttttctaa actaaatttt aaagggcgcg atattttatt
     2281 tttagcatat atcggcacga ttgctgtccc ttggcagtct tatatgatcc cgcagttcat
     2341 tatgatgaga aaagtgggtt taacatatac gttgtggtcg ttggtgctat tacaagcgtt
     2401 caatgctttt ggtgtgttct tgttgaagca atactacagt agtatcccag attctttatg
     2461 tgagagtgcg cggatcgatg gcttatcaga gtggggaatc tatcgcaaga tcattttacc
     2521 tcttacaaaa ccggcgattg ccagtttgac gatcatcaca ttcgttaata catggaacga
     2581 ttatatgggg ccattcattt atttgtcttc tacggaaaat aaaacaatcc agttagggtt
     2641 gaaaatgttt attgggctat ttgatgcaga gtacgcatta atcatggctg cctcagttgt
     2701 ttctgttctt cctgttgctt ttatattttt agcgatgcaa aaatattttg tagaagggat
     2761 cgcttcatca ggaatgaagg aataggaggt gattatgaaa gagcagcgtt ttgacaaaaa
     2821 tgtttatatg gaattctttt attggttgta tcaatcattg gcaagtagcc tttgtttttg
     2881 gtgtgtcaat atcttgtata tagcagctgc gttccttttg tcgatagatg gaaggaatgt
     2941 cttttccttt attttttctt tactttttat tggtccaggt tgcatcgtga tgataggtat
     3001 gacagaagaa ttagcaaaag aaaaaagttt ttcaccagta cgtttgttca taaagacgtt
     3061 tcgccaattt tggctaaagg gattttttta ttggctattt gcttggatag ttagtgtgat
     3121 aatgattttt gattgtttct tttttatacg tttttcttat ggaaaatggt tgattccgtt
     3181 gtttgtttta cttgcttgtt tatcagtttc ctttagcatc aactactggt attttcaggt
     3241 gagaaatcca gcgtcgaaac caaatcaagt tctacggata gcgttctact atacgttgaa
     3301 aaaatggtat gtgagtttac tagatttttt actattgaca agtttgtttc tcttcttttt
     3361 cgtaaagccc caatggtgta ttctactagg tccttctatc gtatttggtt tgatttactt
     3421 caacaatcgt aaattgatga gaactatgga tctgtagact ttataaattg aaactttcaa
     3481 ttaagaatga cagtttttat gtaaaaactg tcattctttt tcattttttg aaacatatgg
     3541 ctaaacaaat gaaataaaac gtgctatact tttgaatgaa acaggtttag aaactgtatc
     3601 aattatttgt gattatttct aactaataat taaaaataaa gcataaaaag gacgatgttc
     3661 aatggacgaa aaaatgaaac aaggttcgaa atctgtagta gaaagtttga ttaatcatca
     3721 tgttgattat gtattcggta taccaggtgc aaaaatcgat ggtgtattta atgaattaga
     3781 ggatcaaggt cctgaattaa tcgtgactcg tcacgaacaa aatgctgcat ttatggcaca
     3841 agcaatcgga cgtattacag gagagccagg agtagttatc gcaacaagtg gtcctggtgc
     3901 tagtaactta gctacaggtt tggtaacagc gacagcagaa ggtgacccag ttttagcaat
     3961 tgccggacaa gtaaaacgaa gcgatctatt gaagttgacc catcaaagca tggataatgc
     4021 agcactgttc cagccaatca caaaatacag tgcagaaatc caagatccag aaacaatttc
     4081 agaagtgatt gccaatgctt atcgtatggc taagagttca aaaaaaggcg cgagctttat
     4141 cagtatcccg caagatgtag tggatgcacc tgtccaaggg aatgtcatca agccattgag
     4201 cgatcctaaa ttaggttctg cttctgcaga tgatatccgt tacctagctg aacgcattag
     4261 agaagcaaaa ctaccggtac tattagtagg tatgcgagga tcaagtgaaa aagaaacgtt
     4321 agccatcagg caattagttg gaaagacagc attgcctgtt gtagagacat tccaagcagc
     4381 tggagtgatc tcacgtgaat tagaagcgca tttctttggt cgtgtgggtt tatttagaaa
     4441 ccaaccagga gatatgcttt tgaaacgaag tgatctagtt attgctattg gctatgatcc
     4501 aatcgaatat gaagcaagaa actggaatgc ggaaaaagat gcgcggatca ttgtgatcga
     4561 tgaagcgcca gctgaaatcg atccttttat gcagcctgaa agagaattga tcggtgatat
     4621 ctcagccact cttgatttat tgacaggttc attagagcct caacaagtgt cagaagatgc
     4681 aaaagaatat ttagcatctc ttcaagcaga attaacggag cgggacattg ttcaatcaaa
     4741 aggagaagca ggtatcttgc atcctttaga agttattaac acgcttcagt caaaagtaac
     4801 agatgatatg actgttaccg tagatgtagg tagtcattat atttggatgg cacgccactt
     4861 tagaagttat gaacctcgtc atttgttatt cagtaatggt atgcaaacgc ttggtgttgc
     4921 attgccatgg gcgatttctg ctgcattggt gcgtccaaat acacaaatcg tatcagtttc
     4981 cggcgatggg ggatttttat tctctgccca ggatttagaa acggcggtac gtaaaaaatt
     5041 gaatatcgtt catttgattt ggaatgatgg acactacaat atggtagagt tccaagaaaa
     5101 aatgaaatat cagcgggcgt caggtgtaga ctttggtcca gttgattttg tgaaatatgc
     5161 agaagctttt ggcgctaaag gaatccgagc tacaagtgta gaggaattag aaaaagcttt
     5221 agaagaagga tttgcgacag aaggacccgt aattattgat attccgattg attaccgtga
     5281 taatgagaaa ttgggagaaa caatcttacc agatcaattc tattaaggaa gtgtcgacat
     5341 gactgaaaaa atcttatacc agcacgggac attaggagct ttgatggctg gcttgatgga
     5401 tggaacagaa acgattgctc atatattaga aaaaggaact ctgggattgg gaacattgca
     5461 tgggcttgat ggtgaagtca tctttttaga tggcgtagct tatcaaggaa gatcagatgg
     5521 ctcagttgcc caattagatg gaagtgaact taccccctat gcagcaatta cagattttac
     5581 gccagatact agtttttctg tctcaaaaac tgcagatgga gaacaattga aaaaagacat
     5641 attagtaaga gaagccgggg aaaacctatt tttggcagtg aagataactg gattattcaa
     5701 aaatatgcac atccgtatca tgccgaagca agaaaaacct tacagaagac tagcaaaaat
     5761 ttcagagtct caaccagaat tccaacaaag caatgtccaa ggaacattag tcgggttctt
     5821 tacgcctgaa ttattccaag gagttgctgc agcgggattt cacttgcatt ttatcgatga
     5881 tacacataca tttggcggac atgtaatgga ttttgaagtg gctgaaggaa aagttgagat
     5941 tagccgcatc tcgtcccttg tacagcattt tccagttgat gatccgactt ttgttcacga
     6001 aacgattgat tatgctgatt tagcagcaga aatccaacaa gcagaataga aaaagacagc
     6061 gagatgcgtt caaggtgatt aacctgacgt atctcgtttt tttgaggagg aaacctagcg
     6121 tttacaaaaa aactgagcta taatcttctc catagatcaa aaaggagcgg tttttatcat
     6181 gtggacagaa aaacagaaaa aagatgcata cgaaatttta ttattacagc aacaaggatt
     6241 attagaagca gaagataact ggttagcaaa tttagcaaat tcatctgcat tgttgaatga
     6301 gactttgcct gagactgtct ttgcaggata ttatttattc gatggtaatg aattgatttt
     6361 aggaccgttc caagggcgag tttcttgcac acgtatcaag atgggaaaag gagtttgtgg
     6421 agaatcagct gaaaaacaag aaacattgat cgttgatgat gtcaaaaccc atgcaaatta
     6481 catttcctgc gattcagccg cacaatcaga aatcgtagtg ccaatggtaa aagaaggtaa
     6541 tttaatcggt gttttagata tcgattgcgg agtaacgaaa gggtatgatg aaatcgatca
     6601 agcatttcta gaaaaatatg tagatctttt atta
//
LOCUS       DAHHPA010000120         6605 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.125, whole genome shotgun sequence.
ACCESSION   DAHHPA010000120 DAHHPA010000000
VERSION     DAHHPA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.125"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(68..994)
                     /gene="glsA"
                     /locus_tag="NBH55_002512"
     CDS             complement(68..994)
                     /gene="glsA"
                     /locus_tag="NBH55_002512"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCD1510450.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1017..2228)
                     /locus_tag="NBH55_002513"
                     /pseudo
     CDS             complement(1017..2228)
                     /locus_tag="NBH55_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2263..3876)
                     /locus_tag="NBH55_002514"
     CDS             complement(2263..3876)
                     /locus_tag="NBH55_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1510451.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(3988..4197)
                     /locus_tag="NBH55_002515"
     CDS             complement(3988..4197)
                     /locus_tag="NBH55_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HCD1510452.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            complement(5186..6400)
                     /locus_tag="NBH55_002516"
     CDS             complement(5186..6400)
                     /locus_tag="NBH55_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCD1510453.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(6528..>6605)
                     /locus_tag="NBH55_002517"
     CDS             complement(6528..>6605)
                     /locus_tag="NBH55_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1510454.1"
                     /translation="FEKYDYRFHWNSDNSVIEIWIPIQD"
BASE COUNT         1848 a         1438 c         1096 g         2223 t
ORIGIN      
        1 aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca gcctctttca
       61 agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc aacaattgga
      121 taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct atgccgtaac
      181 gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt acaccaacat
      241 ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt gatttgacga
      301 ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt cctttatttg
      361 ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag cattgtctga
      421 aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct ttcatatagt
      481 aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt tcttcattca
      541 atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc tcaaattttt
      601 catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag
      661 gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg aaggcaaaac
      721 cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct ttgattgcta
      781 ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc
      841 cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt ttgggatcca
      901 cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag tggaaatttt
      961 ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt cattttttat
     1021 ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat aactaagacg
     1081 ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc tgcgatacta
     1141 gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc taaacaagca
     1201 acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag actgtcatct
     1261 ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag gtagattaca
     1321 acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct taatgtaaag
     1381 acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc aggaacaccg
     1441 tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc tacgtgaagt
     1501 gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa acaaacaaga
     1561 atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa taggaattct
     1621 aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt agtagcgacg
     1681 gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa cattgctaat
     1741 ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt ttctacgccc
     1801 atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga aaaatcagga
     1861 atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac ataatggatc
     1921 cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc gatccgagca
     1981 atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca gaaaataatc
     2041 aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc cgttgcatag
     2101 gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat ctcaaaccat
     2161 tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac ccaagtatag
     2221 acaccgccgg tttcccttag ttaaaaatat tactcttgtt gattactcaa ctcttccaat
     2281 aaatcggtaa tatatctcaa tttcctgtat tctttcgtta tcctcattcg ttgttgcttc
     2341 atgtatgagg attttttcta tcatggcatt taataatggt gctgtcagtt cctttggtac
     2401 ggaatattcc ttgattaact caatccactt ttcagcacct atcatatcct gttccatttt
     2461 acttagttct tctcttaggc ttgttatctg ctgttccagt tctatctgtt ctttttggta
     2521 cttgctggac aacatcacaa aatttcgctc tgttatcttc tcacaggctc tatcttcata
     2581 cattttcgca aataaacggt caatctcatt ttgtctgttc tcggctttct tcaatgtact
     2641 tgcctttttc ttcttttccc gtattctctc tgcattgcca gctttctgta tcttgttcaa
     2701 taccttttct tcgtcctgct gtactgcctt agcccaatac tgcaagcgtt ccaatacggc
     2761 ttgatacagc acatcataac gaatgtagtg catagaacaa ttacctttgc caaactgccc
     2821 gtagtaactg caagcataat aactgtaagg cgttttattt gtcttattcg ttccaaatct
     2881 catagaccaa ccacaatccg cacacttgac aagccctgca aaaatcggcg ttgccttttc
     2941 ctttgtctgt ctgcgtcttg attttatctg ctcctgcaca cgatagaaca cttccttgtc
     3001 aataatcggc tcgtgagtgt tttctactcg aaaccattca ctttcgggct tacgcacttt
     3061 ctttttgctc ttgaacgata ccgtagactg catattgtga acactgtttc cgatataaac
     3121 ctcacttttc aatatcgcct tgacatgagc aatcgtccac tcataacgct tactttcggg
     3181 ttttccctca aagatatgtg caaaagtacc gtatcttgta aaatttagcc atgacgctgt
     3241 cggaactttt tctgctctta actgctttgt gatttttgca cttccgtaac cttgataggc
     3301 tagggaaaag attttttcaa taatccattt tgtttcatca tcaaccaaca gttttccttt
     3361 gatgtcaggg tgtttcttat atcctaacgg agcataagca ccgtaatgag ccccctccgc
     3421 aaactttgtc ttaaatgctg atttgacttt acggcttgta tctcttgcca cccattcatt
     3481 gatgatgttc ttaaatggtg caatctcact ttcgcctttt tcgctgtcta caccgtcatc
     3541 aatcgctatg tagcggacat tatgactagg gaaatacaat tctgtatatt gtcctgtcat
     3601 aatataattt ctgccaagac gggaaaggtc tttcgttaca acacagttga tttttcctgc
     3661 ctctatatcc tcaatcattc tttgaaaact cggtctgtca aaatttgttc ccgaccagcc
     3721 gtcatcaata tattcatcaa tgacattcaa atgatgttct tttgcatata aacgcaacat
     3781 acttctctgt gtggtaatgc tggaactttc gccctgtaat tcatcatctc ggcttaacct
     3841 aagataaagt gcagtattgt aaatctgttg tttcattgtc agttatcctc ctttataact
     3901 aacccgtgtt tacaatacct gcttgcaagt aaagtataac acgggtactt tttgtcattc
     3961 aatctatttc ttacaactgc accgatttta tgcgattgtc tgctttgctt tttccagcat
     4021 gacatcagtt agaagttctt ccattgtttt gcccttttcg gaaaaatgct cttttactac
     4081 aatctttgtc tttcctctgt tgccaatgcc acacttgcca tttttctttt gaattgtttt
     4141 tacagcatta tcaataacac ataccccctt tctgtaaaat ctttctaagc aattcaacgg
     4201 cttttgtcat agcccacagg agttccacct ctgcatagtt cttatgtagc cgtacccatt
     4261 gcgtgcgacg ctcttaatgc tcaagctgtg gctgtacggg agtatcatta tctgacagta
     4321 caggtcatgg cgataaaaga ggacaaatct tttacaagaa cacaaatagg ttttcgctcg
     4381 ctcttgcagg ggaaaggtcg tggcgtattt gtttcaacgg ctagctgtct gtcaaacgta
     4441 ttgaatagct ttattcagtt gtcaaagaac gattgaaaga aaaaaatcgt ctttcctata
     4501 tatcgcgttg gaaaagagga gaaacgtaac caaaatccaa aactttttcc attattagta
     4561 caaatggaac ggttcatttt ggcaatcaac ggttgatttt ctcctttttc actactatct
     4621 gtacagcaaa cgtccatttg ctaagtttaa atggaaaaat tttaacttct tttcatgcac
     4681 catataggga tagaaaatgt cattttgcta agttgatgat gaaaaaagaa tattttcctt
     4741 ttcacaccct aactacccaa accataccgt tactgccaac aatagaatga aatattttct
     4801 ttgcaccata tagggaacgg aaaccttaaa ttgttaagtt gagaacaaag tattttcatc
     4861 ttttcacatc aacactcaac tgctaatgcc tgtttgaata caaaatgatg atgtttttcc
     4921 tcttttcaca gaagactgtc cactaatcac gaatttccgt cattttttca aaactttttt
     4981 cttctttcac ttcatatagg gacagcaagc cccgatttaa tgacctattt tcaaaaaaag
     5041 tttagatttc cttttctcgc accatatctg tacgcaagta gccattttgt tgcagattta
     5101 ataaaaaatt tatttttctt ctttcacttc acatagggaa tgaaaacagc tctttgctga
     5161 ctaaatctga aatttttcaa ttacattaaa aaataccgtc attctcttgt ggaacagcgg
     5221 tatctatacg ttcttctatg gttgatttct ctaattgtaa taaatgttta tgcttgtctt
     5281 ttagttctgc ctgctcaatc atatctttca gtatcgttgt acgattttct ctgtctaatg
     5341 cctgtaccat ttttatggtt ggtgcgactt gtctttgtag ccaatgcaac gctttttgta
     5401 aagtgtaagg ctctggcttt gtggttaagc gtatcggctc tctgttttcc cctacaaacc
     5461 atgcccaatc atcatttgtt atccattgac tttttggctt gtcgtcctct ctgtcaacaa
     5521 agcggacata atgattgatg atagaaaaag cggtgcgttc agcgtcccga taggtcagta
     5581 aatctacaac cgcataatag gctcgttcgt tcttcatgcg tatttcaaaa cggttcttaa
     5641 tctccgtatc ttcaatttct gtgccattct tgacatactg ctcataattt ttctgataag
     5701 cacacatata taattcgcta cttgttgaac cgagatataa ggtttctcct gtattctttg
     5761 gtaaatcgct accgcatttt tctgtaccgc tgtaatcttt ctgcatacgg aagtaggaca
     5821 tacattcgcc agccttgtat ttttccttta actttggaat atccaatatt cctgctttat
     5881 cattgatagc caaatcaagc cgtttcatca caccaccagc cgtcatacaa tccagcatga
     5941 agtcatacca tgagcgttcc tgtgccagca gataacattc catttgccga cagcctttgc
     6001 ctttcagttc caataaaaca cctaaatgct cctgcatgga acacatgata ttgatgtcgc
     6061 caagtacata tttgctttca tatccgtatt ttccataatc ttcataaagc atgtaattag
     6121 atttcagttg taatacatca cggataatcg ccaatgcgtc cgtagtagga aagcggacac
     6181 gaaagtaatc aatcaataag aatagtggtt cttgcggatt gaaacgctca atctgttttt
     6241 ctatgatttc tcttaattcg tcattcaatg gctgttttcc tttttcaatg cggttgtaat
     6301 attcacggct gataccacag gcaacagcaa gccttgtttg tgttactccg tattcctcac
     6361 gcttttgtct tagttcttca ataaagtttt tatcattcat tcttcaattc ctccaatcaa
     6421 aaaaggcaac taccataatt gataattgcc tgtcattaaa aatctgtata gaaaaagcag
     6481 tcaatcctaa gactaactgc tttgtgtgta tggatataaa attgcttcta atcttgaata
     6541 ggtatccaaa tttcaataac tgaattatca ctattccaat gaaaacgata atcgtatttt
     6601 tcaaa
//
LOCUS       DAHHPA010000121         6320 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.126, whole genome shotgun sequence.
ACCESSION   DAHHPA010000121 DAHHPA010000000
VERSION     DAHHPA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.126"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            827..1561
                     /locus_tag="NBH55_002518"
     CDS             827..1561
                     /locus_tag="NBH55_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1510455.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1577..2746
                     /locus_tag="NBH55_002519"
     CDS             1577..2746
                     /locus_tag="NBH55_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCD1510456.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2762..3757
                     /gene="lacD"
                     /locus_tag="NBH55_002520"
     CDS             2762..3757
                     /gene="lacD"
                     /locus_tag="NBH55_002520"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCD1510457.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3836..4762
                     /locus_tag="NBH55_002521"
     CDS             3836..4762
                     /locus_tag="NBH55_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCD1510458.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4781..6032
                     /locus_tag="NBH55_002522"
                     /pseudo
     CDS             4781..6032
                     /locus_tag="NBH55_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            6163..>6320
                     /locus_tag="NBH55_002523"
     CDS             6163..>6320
                     /locus_tag="NBH55_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD1510459.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTAL"
BASE COUNT         2118 a         1048 c         1289 g         1865 t
ORIGIN      
        1 cgcagcgaat ccttgataat gagctcggat gtgctaacgc tagcgaggca gtctaacttt
       61 aactgttagc tcctgccgaa gatcgtcaca tccgaggagg ctcattgtca aggacaatcg
      121 gaatagcata cggaaagaag ttctgcacct tataaaattt gtcaaaaaaa ctgttgccat
      181 tccaaagttt gaaaaatgct atttcgaggt agaaggatat tatataagca cggttgacta
      241 aacgaagtga taataaaaaa agttattcaa gaataagaaa aattatgata cgaattcaga
      301 taaactggag aatacagaga cctttaaaag ggataagaaa caacataaat tcctcttaag
      361 agagaacgaa aggacaaagc cagcaacttg atacacgagc tagtattaga ggaatttatt
      421 ctcatagact cccttttgaa tacgtttcaa ctttaatttt catgttcata aatgatttga
      481 aaccaaagca aatgcgttgc acttttcctc cgtcttccga aaatcgttca attcactggt
      541 aagtagtcat ttgatagttt gaggcaaatt gttgattgat gaagggtagg agctaaaaaa
      601 agataccagt tgaagagaga aaaagaaaaa aatagagggt gttcttgaat gaaaaaagtt
      661 ggaaaattat ttttcatttc cttttattcc tattaaagtc agtgatgtca atcatattaa
      721 caagactaaa aatgaaaata aaaaaaagat tgttcttagt attgactcat aaaaaatacc
      781 actactatac ttacattgtg gttatagcca gttaggagag acatcgatgc aaaatacctt
      841 tcaatcaaaa tcactttatt atcagctggt agaaatattg aaacaacgaa tcgaaaacag
      901 tatggtcgct catgacaagc tgccatcaga aagagagttg accagttttt atggcgttag
      961 tcgcacgacg gtgcgactag cattgcaaga actggaaaaa atgggctaca tttatcgaca
     1021 acatggaaaa ggaacctttg tttcggaatt aaggacaaat gcgactgatc tagctggcgc
     1081 atatagtttt accgaacaaa tgcgggcaat gggcaaacat cctgaaacca agatattggc
     1141 cttcaagaag attgaagcaa ctaaatattt agcgagtcat ctgaatgttc aactaggtga
     1201 gcctttattt caattagacc gattgcgctt agctgatgat ttgccaatga tgatcgagaa
     1261 atcctatttg ccagtgaagc tatttatgtc cctttctcaa gaaattctat ttctgaaacc
     1321 tttatataat ttgttttcag agaatttcaa tcaaatcgta cggttggctg acgaggaatt
     1381 ttatgccagc attgtccaga aagaagatag tgagttgcta aatattccag aaggatcacc
     1441 agtgctccat ttagttcgct caacctataa tctcgagaac gaagtgattg aatttacatt
     1501 aagtgtagcg cgggctgata aatttcatta taagatcagg catctgcgtt ccactaactg
     1561 aattggagga aagaaaatgt ttatgttctc aaaagaagaa ctacgaaagc taggagcaga
     1621 aaatactacc cgagaaattc gacagcaacc ggaactgtgg catgagacgt ttgaccgtta
     1681 tcaagaaaca gttgcagatt tagatcgttt gtttacggat gcgaaacaac atgctggaga
     1741 gcgaccaatc cgagtcattt ttacaggggc cggaacctcg caatatgtgg gagatacact
     1801 aattccgttt cttactagtc aaggaaatac gaaccagttt atttttcaaa gtattggttc
     1861 gaccgatatc gttgctacgc caaaagaata cctgattcct gatgagacaa cgatcttggt
     1921 ctcatttgcc cgtagtggaa atagtccaga aagtctagcc gcagttaaga tggccaatca
     1981 gctggtcaac aagatctatc atatcgtgat cacctgtgca ccagacggat tattggctac
     2041 acaaatgcct acaaaaaaag aaaatctagt tttactaatg ccaaacaaag ccaatgacgc
     2101 tggttttgct atgacaagca gtttcagctg tatgacgctg actgcgctac ttatttttga
     2161 tcaagcaagc aatcagcaaa aagaaagata tgttcatcaa ttaagccgac taggagaaga
     2221 agtcatccaa cgagaatcag agatccaagg gtatctggca tcagactttc atcgcatcat
     2281 ttacttaggt tcgggaagct taggcggatt gactagagaa gcgcaattaa aagttctcga
     2341 gttaactgct ggaaaaatcg caaccgtatt tgatacatct ttaggattca gacatggtcc
     2401 caaatctttt gtcaatgagc aaacattggt tttcctgttt gtcagcaatg actcatatac
     2461 gcagaaatac gatgtagaca tgctggaaga aatcaaaaat gacggtattg cccaacgcgt
     2521 tgttggaatt ggtcaaaagg gttcattgtt tagtggtgat tcctttgaat tagttgcaga
     2581 ttcgttactg ccggatggct atcttgcttt agcagatatc atggtagccc aaacgatcgc
     2641 attactttgt tcggtgaagg ttggtaatac accagataca ccatcgccaa gtggaacagt
     2701 caatcgtgtg gtcaaaggcg taacgattta tccatatgaa aaataggaaa gagtgtgaag
     2761 catgttagaa tggtcggaaa ataaaagaca agcaatggaa cgtctatcga cgccagaagg
     2821 cattattacc gctttggcga tcgatcagcg aggcgcgctg aagaaaatga tgtcggcttt
     2881 agggatggaa gcgacaaagg aaaaaattga gcagtttaaa acactaattt cccaagaatt
     2941 gactccttat gcctcagcaa ttttattgga tcctgaatac ggactgcctg ctgcacagaa
     3001 gcgtgcagca gcgagcggtc tgctgttggc atatgaaaaa actggttatg atcaaaccgt
     3061 acccggccgc ttgccggatc tgctgtcgga ctgttcagtt ctgcgattga aagagttagg
     3121 tgcggatgcg gtgaaatttt tactgtatta cgattatgaa gaagcagagg aaatcaatcg
     3181 gcaaaagcaa gtttttgttg aacgaattgg aagcgaatgc atggctgaag gactgccatt
     3241 ttatttagag ttattgtcat atgatgctaa aaatgtagat acgaaatcgc tttcctatgc
     3301 caaagcaaaa ccgcgcaaag tgatcgaaat gatgaaagaa ttctcgaaaa cccagtataa
     3361 agtagatgtc ttaaaggttg aagtaccagt aaacatgtca tttgttgaag gaaatgcaac
     3421 aggggagatc gcctatacac gtcaagaagc tgccgcttat tttaaagaac aaagcgatgc
     3481 aacagatttg ccgtatatct tcttaagtgc tggggtatca gcggcattat tcatggagac
     3541 attgcgtttt gccaaacatg ctggggcacg attcaacggt gtactctgtg gcagagcaac
     3601 ttggaaagat gcagtcgctc ctttcgttga aaaaggagaa gcggcaactt tagaatggct
     3661 gacagaacaa gggacacaaa acattcgtca attgaatgaa gtgataagag aaactgccat
     3721 cccatggtat gagaaagtaa gtgaaagcac ttgttaatgt ttctaatata ttagtagtga
     3781 taagagatat gtgtgattat aatttatcat tattgaaact gggaggatat tttatatgaa
     3841 taaaagattg ttagattatt cagcttcaga ttttttgaaa gctaagccga tggaattaaa
     3901 gcaggcaatt ttggcttctg aaggtagaac aattatggca gaaaatgtgc caagtagctg
     3961 tccttacatg ggaaaatcag tgacaaatgc agaattggag agagctgctg gagctgattt
     4021 aattttgttt aatgcactag atctttttaa tcctcaaatt tcaggtgtac ctttaactct
     4081 aaaagagaat ccaataatct ggattaaaaa agctttagga agagctattg gtgtaaattt
     4141 agaacctgta gacaaagaag ttttgatgag tgaaaatcga actgaaattt caagtggtag
     4201 aatcgtttct cctgagactt taaaaaaggc aaatgaatta ggctttaatt ttatttgttt
     4261 aactggaaat ccaggaacag gagtatctaa tagaactatt gagaaagcaa taacattatc
     4321 caaatcaatt tttaatggat taattatcgc tggaaaaatg catggtgctg gtgttgatga
     4381 accaattatg aacttagata tagcaaaaag atttattaaa acaggtgtag atattttatt
     4441 agttcctgct ccatacactg ttccgtattt catggaaagt gatttgcgag gaatcgttga
     4501 ttatataaaa gattttaata gaaataaatc tattgaggaa aaagttctag tactaacggc
     4561 taatggaaca agccaagact cttctgacca agaaactatt aaaaaaattg cattagcatc
     4621 aaaagcatgt ggagctgata ttcaacacat aggggattct ttaaatggta tttgtcttcc
     4681 agaaaatatt tttgctttgg gtcaagcaat tcggggatat cgtcatcaaa ttattatgtt
     4741 aggaaaatct aattatcgtt aattaaggag gaaatgaaag atgatcgaca aagttcaaaa
     4801 aatagctgca aaaattagta atcaaagaca tctactagct ttaagagaca gcatggctgc
     4861 agttatgcca ttaatcatta ttggttcctt ttttatgtta ttagcaaatt tcccgataga
     4921 aggatttaca aaatggttat ctgaaattgg agtacaatcc tttttgaaca aagcatcaga
     4981 ttcaacattt ggtattattg gattagctgt tacctactct ttgggacaca atctagcaaa
     5041 gacttatggt gtagactcat tatcagcagg attattatct ttgagtggat atgttttact
     5101 tacaccttta ttggatgcag atggttctat aggatttcca acaaaatact ttggtacctc
     5161 gggtcttttt gttggtatta ttgtagcact tgtatctaca gaaattttcc ggtggtttgt
     5221 acttaaaaat attacaatta aaatgccggc aagcgttcca ccaaatgtaa gccgagcttt
     5281 cgttgctatt attcctggct ttttttgtcg ttctactatg gtttattgtt gttgtaattt
     5341 gttataaact tggaattgaa aatgttcatg ctcttattgc agatacactc gcaaaaccac
     5401 tatctttact gacgaaaact ttaccaggaa ttatattggt tatacttatt caatgtttct
     5461 tttggatgtt tggtattcat ggtgcacagg taacggggcc aataattgaa ccgttattat
     5521 tgcaaaactc tgatgtaaat agaattgctt atcaagctgg aaaagaactt cctaacatta
     5581 taacttatga attcttatat aactttgttt tttctggtgg tgcaggttgt ttatttgcac
     5641 ttgccatttt aacatttttc ttttcaaaga gtcaggaaaa taaaacttta ggtaagttat
     5701 caatcgctcc agtaagtttt caagtagcag agccccttct atttgggttt ccaactatat
     5761 taaatataaa aatgttaatt ccatttgtaa ctgcaccagt agtaacaaca ttaattactt
     5821 atttttcaat gagtatggga ttagttgcaa aacctgtagg agcaacaatc ccatggacaa
     5881 ctccaccgat aattgccgga tttttagctt ctggtggacg aataagtggt gcagtgattc
     5941 aagtaataac aattgcaata aatgttttga tatactatcc attctttaaa ttagatgata
     6001 atgccaagct aaaaagcgaa aaaaatgatt agaactacct atatgtttct aactgtagtt
     6061 aagtatcgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat gttacactca
     6121 aattgtaata cccgctaaag catacaagga gagtgaacat aaatgactta tacccatctt
     6181 acaccaaacg agcttgtaat gatagaagca tattttcatc aagaaactcc ggttgctatc
     6241 gttgcgaagc agcttaaacg tggacgccaa acaatttaca atgtctataa ctttctcaaa
     6301 tgtggtggaa cagcacttga
//
LOCUS       DAHHPA010000122         6287 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.127, whole genome shotgun sequence.
ACCESSION   DAHHPA010000122 DAHHPA010000000
VERSION     DAHHPA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.127"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..862)
                     /locus_tag="NBH55_002524"
     CDS             complement(<1..862)
                     /locus_tag="NBH55_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510460.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRNVQITPVSF"
     gene            complement(865..1221)
                     /locus_tag="NBH55_002525"
     CDS             complement(865..1221)
                     /locus_tag="NBH55_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510461.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1519..2493)
                     /gene="sgrA"
                     /locus_tag="NBH55_002526"
     CDS             complement(1519..2493)
                     /gene="sgrA"
                     /locus_tag="NBH55_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCD1510462.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2689..3528)
                     /locus_tag="NBH55_002527"
     CDS             complement(2689..3528)
                     /locus_tag="NBH55_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1510463.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3713..4600
                     /locus_tag="NBH55_002528"
     CDS             3713..4600
                     /locus_tag="NBH55_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCD1510464.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5184..5879)
                     /locus_tag="NBH55_002529"
     CDS             complement(5184..5879)
                     /locus_tag="NBH55_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCD1510465.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(5863..6261)
                     /locus_tag="NBH55_002530"
     CDS             complement(5863..6261)
                     /locus_tag="NBH55_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCD1510466.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1992 a         1253 c          926 g         2116 t
ORIGIN      
        1 caaaggatac aggggttatt tgtacgttcc tgactaacat acagtattga aaaatcaacc
       61 gtctgttctt ttcataataa gtattaagct ctggaattct gcgagctacc ccactaaaaa
      121 actgttgcat gattggatac atcactggaa atatatgctg aaaatctctg tcttctgctg
      181 attttccaaa agcatccacc ttcttttttg tcccaaaaac ccttgaatac aaatggatca
      241 ttgccaaatt gacatataga taattttttt ctgaagttgt aaaacaaatc ttgaagtttt
      301 tttcaaaatt ttctacaata gacgattcaa tccagcccca actttcttgt actttatctg
      361 gagtatccaa caaatcttct tccaaatagg tgttcattac accaaacata aaataaaggt
      421 aagcttgctc tgttggagtg atgtgattca tcgaccagaa gtttgcttca aaaaaaggct
      481 ctagtatgta atgcgtaaac tcttcttttg ttatcaatgg atgcacaaat ttgttcatct
      541 cttctgggac atacgtaatc gagtggcctt gtttcattcg ttgcaacgaa attcttagta
      601 ggctgatcca tttctttcta tctatactac gcgtatatgg cgcatgtttc gcaaattttt
      661 ctaatagttg acgtttattt tttatacttg ataatatatc agaatgattt tcgtcgaatg
      721 actgccaata catcaagtga aaaaagtatc ggatacggta ttcttctcct tctatcggat
      781 tacctttttt caagctgcaa gataaatgat agtttttcaa aatagtatta agttctctta
      841 attttctttg aatcgtacgc aaacttatat aattttttga ctaagatttt ctaaggattg
      901 ataagtacct ttgtaaagtg aatccagtaa aatataacca atcgttccct ctaaaagtat
      961 agaataaact ttactcagta aaaactgaga agaaaactcg acttttatca tcttgttttc
     1021 tgaaagaaaa gataatttat attctggata cctttcgcaa aacgattctg cttgttgaat
     1081 caccgaattc atcttagcca gggtgtagtt tagatctaaa gcgatatcct tcaccacata
     1141 taatttctcg tctctttcat aacaatagtt caaaaattga tatacttgcc tttcattgat
     1201 atcaataaat tcttcaaaca aagtacatac tcctttttat ctattattta gacttattcc
     1261 gacagctaca tgaataacgt acaaatagat tcgttactga tttttcagag atactaccat
     1321 gcgcagtgtg cattgtcaat tattcttgct cttattttcc atttgtgttc ctgttatttc
     1381 ctgtttctca aatatgttag taaacaaaaa tagatctggc aaagctacaa cttcgccagg
     1441 caatacagca ctctcgaatg aaatattcac tcaaaaatct atcgttttat tcaattttag
     1501 atctgttgct tttattcttt atttcactcg atggaagaga gaacgaaaat agcttgctcc
     1561 tacaatcaaa atgagtaaca cacccatcca tgtaattagc ttgttcaagg ttctactacc
     1621 agtttctggt aaatcgcttt ttctattatc tgtttctttc gctttttctt gatgatcaat
     1681 tccacgagaa acaaaatcag gctgttttag cacacttttt aggttgctcc ctgcatttct
     1741 cttttgttcc ttagttggta tgataggtac caaatcaagt tcattattta cttttcctgt
     1801 gctgctttcg ctgctcgtgc ttcctgtgct actctcacta ctcatgcttc ctgtgctact
     1861 ttcactactc gtactctccg tactgctttc gctgctcgtg cttcctgtgc tactttcact
     1921 actcgtactc tccgtactgc tttcgctgct cgtgttttct gtgctacttt cactgctcgt
     1981 actctctgta ctgctttcac cgctcgtgtt ttctgtactg ctttcactac ttgtgctatc
     2041 cgtactgctt ggcggtccaa caactgtcca actgcttggt ttcaaattct ccgtgatcgt
     2101 catctgtcca tcataaaaaa caatcaaatc aactttatct ttactcttga tctcatcaat
     2161 cgatagttct gcagaaatca ttattgtata ctcaatacca gcggtccatt gtacgtcttt
     2221 taaataaact tccccaataa ctttttctga acttccatta ccatcccaac catatgttag
     2281 aacagcatgt acatcactag cattcacgct tttgtttggt ttgactggaa tagtaatgac
     2341 ttgttgtgtc atgtcataaa caatccgatc agttttcaca gtaaatgaaa gattctcatt
     2401 tgcccgttca tttgcagaag tactgattcc aatcattgct gcaattatac tcagcccaag
     2461 aataacaaat aatacggtcg ctgttttctt catttctatc actctccata taattacctt
     2521 ctatttgata aaggaattat ataaaatgaa aatgatgaga acacgacagt cagttgtgaa
     2581 aatctaacaa aagatgtcgt gtattcgctt gaatttgttt actttttcgt ttaatcaatg
     2641 acagacttaa aaaagcaaga ccgatatcag ccttgctttc ttcatctttt attgataaac
     2701 agcagtgtat ttcttcatcc gctttgtttt atatgcatag agtaacaaac caacaatcaa
     2761 gactaacaag aataatgtag ttgatataga tgcttcagcc ccaaagtttc ccccagtaat
     2821 tattgtattt tgcgattgga tcagagtgga gaaaaaacct tgttcttgcg ttccgctgac
     2881 tggtataccc aaaatagctc cttgtgtcca attccaagta atatgagcac caatagccgt
     2941 aaataaactt cctgtactgt agataagtgc gctgaaaaag caacctgcta aaaagatatt
     3001 gaaaactgcc agaaatgtca tgttaggatt caatccgtgc aaaagagcaa ataatgcagc
     3061 agatccaatc attgctatta acggaccaaa tctaagcatc tgatgctgaa aaaagccacg
     3121 gaataaaagc tcttctgatg cccccactgc aataaataaa ataaaactcg ttatcagcgg
     3181 cacaatgttt gcgtttcctg aatctaaccg atacgccccc gtcaaatata atggactagt
     3241 aataagcaga aacactccaa ttccaacaac gagacctccc gctaaatacc cccagtcttt
     3301 tcctcgaaca gaaacaccat aatcttctat tctttttcct tcgaccttac tagcaagaaa
     3361 taaagctagt aaaaaaacag ctaaatgtcc gaccgtaccg gcaattgctc caatccattc
     3421 actatttcca ataaacatat ccgcaagtgc attattgaca agaattccta aaacaaaacc
     3481 aactaagact attaaaacag atccaatcaa ccgaataatc ctgatcatct ttcatccctc
     3541 ctttatatta agtttaacaa cccttttctt tcttgtctcg tgaaaactat gtaaatgttc
     3601 tccatcatag agagaataga tttttacgaa ctaagaatat ctacagcaac cttacgaaaa
     3661 tgaaagactg tctccctaat tcttctgtta gactgttttt aataaggaaa ggatgggaaa
     3721 ttttttgaaa aaacaactac taattatgtg cctttcagca tgtgcaattt tactattctc
     3781 aggttgtagc aagtccaatg aaacgattgc aacgtttaaa ggtggaacac ttacagttca
     3841 ggatttttat gataacccaa aagttaaatc aaataatcaa acactattaa aaaaaatgat
     3901 tgtcttcaaa gcatttgatg atgtatacgg aaaagagatt tcttcaaagg aagtaacaaa
     3961 agaatataat gaacaaatca aaaaactcgg tccaaactac aaggaacaat taaaagcagt
     4021 tggccaaaca gaagagacat ataagaaact ctttaaacag atgctggctt tccaatacgg
     4081 tttgaaagcc aatgtaaaat taacagataa agatctagac actgcttgga aagaattcta
     4141 tccagaagtt agtacacaga tcattctatt ttcgactaaa gaagaagcag ataaagcaaa
     4201 aaaagaagca aatgaagggg aaaatttctc caagcttgtt caagcttatg gaaaaaataa
     4261 aacattaaag gaaactgatg gaaaaatgaa ttttgattca accaatccag agattcctac
     4321 tgaagtaaaa aaagcagcat ttaaactaaa aaacggggaa gtttctgata tcattcctgt
     4381 aacagatcca acgacttatc agcaaagcta ttatctagta aaaatggtaa aaaaacagga
     4441 taaaggttct aataaagaca aatacaaatc tgagctagaa aaaattgcta ccgaagcaaa
     4501 aactcttgat acggaattca tggataaaac aatccgtaaa gtgatgaaaa aagacaatgt
     4561 aactattaaa gatccttatg taaaaaatat attcaaataa acaaatttga cccaattggg
     4621 gatgtggcaa aatttgagaa gtgatttttg gaaattcctt cttatttttg aggcaaacca
     4681 cttttgccac aacttcatcg gggccttttt ttatttgtac caaaacaaat cactggattt
     4741 gttacaatca tgaggatata atttaaaaaa gttttttatt tctatcacat gaatcaatgc
     4801 gccattaagt agattctttc atgcttccct attttttcac ttgccgtatc gcttcaaaaa
     4861 caactatcat aaactcaaac tcgactgaaa agttatttta aaaaagacta aaaatatccc
     4921 ttctggactt gttcctctta ttctgttata ctagagaaac acgaaagata tttttatgcg
     4981 acacttttca tttgttttta tgaaatttat tcaaaactgg cgtaacttca gagttacaag
     5041 gatgtattag aggttaggga atacatcgtt taaatgagaa ataagtaata gtcacccaaa
     5101 ctttaaatta atgaatattg aatcgtcaga tagcctcctc gagttttcga gaaggctatc
     5161 gtttatttta tggagaaatt gattcagaag taatcagcga ccttttttag ttcaccaatc
     5221 gtttctgctt ggaaagtttg cacccaatct tcttggaagc ccctcactgc tttatcaatc
     5281 gagggcatct ctaatacttg atctagtagc tctttaccta acgttatcgc atgagctcca
     5341 gatcgaatgg ctgtagtgac ttgttcacaa tttttgaaac tagctgctaa tatttttgtt
     5401 ggtctttgcg tacgttcgat ctcagtccgc aacgaatcaa tcactttcat agcatcaatg
     5461 ccttgatttt gcatcctgtt ataataaggt gcaatataat ctgctccatt attaatagct
     5521 aaataccctt gtaattctgt gtaaatagct gtaccggtaa tcaaataacc ttcttttttc
     5581 aactttttga tcgcttgaag gcctgcttga ttcaccggca ccttgataaa cgtatctttt
     5641 cccagttctt ctatgataac tcgagcatcc tctatcattt cttcttccgt tcttcctatt
     5701 gtctgaatat ggataggaat ctgtccgatt atttttttga tttctttcat atgcgagaag
     5761 aagtttgacg ctccttcttt tttcagaatt gtaggatttg ttgttacgcc tgttaaattc
     5821 aaaaactgtt gacaagaata aatacttttt gtatttgctg tatcaagcat gatttccata
     5881 tagctttccc ccattctgat cctcttgatc gattttaatg attgtcccct ttcttaaatg
     5941 cggcatttcc ttatcttcta catacattgt tcctggaagt tccggctcat gagatccatc
     6001 aaattttatc gtgatatgtc ctaattcatt caaattagtt tggacaacat ctcctactgc
     6061 tgtgattaca tacatttgtt tatcaaaaca gagcttcata tcacaagtta tttcttttct
     6121 tacctgattc aattcaatct tataacaata atctgttaat gtttccggtg cttcatctcc
     6181 aaataaaatc attatttttt ctttcctgaa tacttctgct tctataccaa catctgttac
     6241 aaccgtttca aagatcgtca tctctgttcc tcctcgtaaa atatcat
//
LOCUS       DAHHPA010000123         6258 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.128, whole genome shotgun sequence.
ACCESSION   DAHHPA010000123 DAHHPA010000000
VERSION     DAHHPA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.128"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(99..1229)
                     /locus_tag="NBH55_002531"
     CDS             complement(99..1229)
                     /locus_tag="NBH55_002531"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCD1510467.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="NBH55_002532"
     CDS             complement(1878..3080)
                     /locus_tag="NBH55_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1510468.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="NBH55_002533"
     CDS             complement(3140..3343)
                     /locus_tag="NBH55_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCD1510469.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="NBH55_002534"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="NBH55_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="NBH55_002535"
     CDS             4880..5692
                     /locus_tag="NBH55_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510470.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1854 a         1285 c          996 g         2123 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaa
//
LOCUS       DAHHPA010000124         6210 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.129, whole genome shotgun sequence.
ACCESSION   DAHHPA010000124 DAHHPA010000000
VERSION     DAHHPA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.129"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(78..755)
                     /gene="nth"
                     /locus_tag="NBH55_002536"
     CDS             complement(78..755)
                     /gene="nth"
                     /locus_tag="NBH55_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCD1510471.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(762..1460)
                     /locus_tag="NBH55_002537"
     CDS             complement(762..1460)
                     /locus_tag="NBH55_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCD1510472.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1728..2912
                     /locus_tag="NBH55_002538"
     CDS             1728..2912
                     /locus_tag="NBH55_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCD1510473.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2918..3361)
                     /locus_tag="NBH55_002539"
     CDS             complement(2918..3361)
                     /locus_tag="NBH55_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1510474.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3587..3874)
                     /locus_tag="NBH55_002540"
     CDS             complement(3587..3874)
                     /locus_tag="NBH55_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510475.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4045..5397)
                     /locus_tag="NBH55_002541"
     CDS             complement(4045..5397)
                     /locus_tag="NBH55_002541"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCD1510476.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5670..6116)
                     /locus_tag="NBH55_002542"
     CDS             complement(5670..6116)
                     /locus_tag="NBH55_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD1510477.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1669 a         1244 c         1037 g         2260 t
ORIGIN      
        1 aaaaagataa acgaaacagc ttttctccat aataacaaga taattcatac atttatttgc
       61 aattaaagaa accacgtcta caggcgtggt ttctttttta gcgccttttc ttttagacgc
      121 atccgatttt tcccatcttg acatattgat agcaaaggac atacctcgca cttgggattt
      181 cttgccgtgc aatgatatct accaaaaaaa atcagtgtat gatgtgtctt tacccataat
      241 tcttgtggta ctttccgcat cagtgtttct tctacttcca tgaccgtagc atctagtttg
      301 cagatcctca ggcgttttga gacacgttcc acgtgggtat ctactgcgat tgctggtatc
      361 ccgaatgcat cccccaatac gacatttgct gtctttctgc ctacaccagg aagagacatc
      421 aattcttctc ttgacgttgg cacttgccca tcgaaacgtt caatcagctg ctgtgcacac
      481 gctttgatgt tttttgcttt atttcgatac aaaccaatcg tttttatttt aagaatgatt
      541 tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa aagaggcaaa caaatctggc
      601 gttgctttat tcactgatac atctgtcgcc tgggcactta agatcactgc aatcaataac
      661 tcaaatggat tattgtgctt gagttctcca tgtgcttcgg gaaacattcc atacatcgtt
      721 tctagagctt ccattgtttt ttgtttgctt aacatattcc gctactcctc caaccaattt
      781 ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact gttctacttc tctttgaaga
      841 agctgctgtt ttcttcgttt ttggtcttct tccacttgtt gttttgtacg tagatttttt
      901 ctttcccacg atagcaaaat gcggtcaacg taattaaaac tataagcctg atttaatacg
      961 gcttcccgca gtgctagctt caaaatttcc ggctgataat ggtcttcttc tagccattgt
     1021 ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa attcttgttc aaataattga
     1081 tacactgact ggatttcttt ttcttgtgtt ttttcttcgt attttttttc ttgtgttttt
     1141 aaatactcac ctaatagatc atagatgggg taaagattat atcgatctgc ttttttaccg
     1201 ttgacaatcg tcgtttctat cttgataaat ccatttgtta cgagacgatc cagtatttga
     1261 aaaatctgat cttgtttgat tcccatatcc atagcaatca tctgcaaatc agggaaagta
     1321 tctccttcaa gctgtgccat atgcaactgt agaacaaata aaaattcttc agattgtaga
     1381 ccgatcctat gataattttt aagtaaaaga ttagatatga ctgtttgtcc agcgcttaag
     1441 taattttcta aattcaacat gatttctcct ccgctcaatc acagtatacg taaaaaaaac
     1501 atgggaagca agaacaggtt tcttttctat ttacttcact gatcggtcag atttacaatg
     1561 tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt atcttcactt ttcggaataa
     1621 ataaattaag tagatttcgc tgttccttag aatgttgaca aggaaacaaa attactatac
     1681 actatttttg tttccaagtt aacattattg cataaaggag tttttacatg tacagtttca
     1741 caaataaaca ttctaaagtt tttggatacg tctctgtctt attcactgga ttaggattta
     1801 ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta ttctcactcc atacatcagc
     1861 aagctttcta tatcactttg ctcatgtctg tttatgctct agctgccttc ttatctgccc
     1921 ccattctcgg tagcttaagt gatcattttg ggcgtcatcc gatccttatt atcagtttat
     1981 taggatcaag tatcggatat ttgctttttg gtttaggaaa ttcgatatgg atgttgttcc
     2041 ttggtcgtat cattgaagga ttgacggcag gagaaatctc caccttatat gcttactttg
     2101 ccgacatcac ggaacctaat gaaagaacca aagtttttgg atggatgggt gctttagtcg
     2161 gtataggaac aacgctgggt cctattatag ggggtctatt agctgagttg gggaattctg
     2221 ttcctatatt tattggagca ctctttactt tcttaaatgc cgtttatggc tacacattta
     2281 tgctagaaag ccttccaata aaaaaacgtt cagtagattt aagtttctcc catgtaagac
     2341 cttttcacca attacaacaa ttattcaaga tttcatccgt tattccttta ttgacagcag
     2401 gttttgctgt ttggttggca gctggctctc ttcaatctat cttttctcaa ttttcgattg
     2461 acgcctttca gtggaaggct gggttggttg gtttaagctt ctctttgatt ggaatcttgg
     2521 atactgtctc gcaattgttc attatgcctc gattgctcaa aaaattttca gaacagcaga
     2581 tcacaagaat agggatgttc agtgaaatcc tcgcctatct attcatcaca ttatctggaa
     2641 ttcttctact gccgattttg tttttatttg ggattatctg ttatggtttc ggtgattcta
     2701 tttttactcc tgtattcaat ggccaactat ccaattctgt ctcagataac cagcaaggtc
     2761 ttgtaatggg cggtacccaa agtatacaat ctctatcgcg cgtcatcggc ccgctaatcg
     2821 caggacagct ttatgctgca gcaccatttc ttccgacggt attcggattt ttgcttatga
     2881 tttttgccta ttatcactac aataaaaaat agtagcttca tttgctactt cgtataaatt
     2941 ctctgtatcg accgataaaa cgaaatactg tgtcaaattc ttcttttgac atattttcaa
     3001 atacagtagc atctcgctgt acccattttt gatggagttg ctcgtgtcta ttccttattt
     3061 tttgtccgat tggcgtcaat tgaaaataga tttctttttt attctcttcg ctttgataag
     3121 tgtttatgat ccctctagct atcagttttt ttgttagctt actgatggct cctctagtca
     3181 tgtaactagc attcgctaat tttgtcacgt taggatcagg gattttcccg atatgttcaa
     3241 tcacttctag ttcatttaga gtaaatcctg caaattcttt ttccatttct ggtttattac
     3301 gatgaataat ctcattagct aaatccagat attcttctat tagcagttgt cttttatcca
     3361 tttctacacc ttctttttct ttcattatat tgtttttttg tttcctattc aacaaagata
     3421 aaaatatttt gtaggaaaac ccaatctgaa accaagaaaa aaaggggttg acaaagcttg
     3481 tcataacccc tttttctgat taaaagatag taaagagaca gtcatcggta attgtgatga
     3541 ttttttgtca tcacttaagg atgagcggcc ttttcaaaaa ctgtctttaa agatcatcat
     3601 cagtttctag cacatatggc tgaccttcat atgtgtactc tgtattcaat gtgccgtcat
     3661 tttcatctga gtagatgcgt ctactgaaag gaaaacgatg agaaacatta tctttggcgt
     3721 catttgctgg catcgtgtaa tattcctctt gcgtttcatt caactgagag agtacttgat
     3781 ctgccaactc atcaatattg atcgttaatt tcttcgaagc acttttataa tcttctaaaa
     3841 atgcatcttg ttggacaaaa tattctttat tcatcttctt tctcaccttt cttttcaaaa
     3901 tcaaataaag ctgcttgttc ttttatgtaa cagctttatc tttgctgctt attgtgacaa
     3961 aaaattcgaa gaaagacaac taattgtact tttcacaaaa aaagacagag ggtcgtgaca
     4021 aaattgtgtc acaacccttt ttctttattg tgcagtttcg atttcgccta gcgctgcttg
     4081 tgcaagtagg ttcaagtaat tccatgggcg gtcaaaatgt ggttggaaga agaaatctga
     4141 aagtgcgaga tcttctacag tcattttatt ttgaacagct aaagacatgg tatttgctga
     4201 ttgtgtaatg tcgtatttgg acatgaactg agcacctacg atacgattcg ttcctttttc
     4261 atagactagc tccatcatta ctttttcagt agttggcatg aactctggac gataattatc
     4321 ttcaaataca gtcgcttgga cttctagatc gttcaaagtt gcgctttctt tggtcacacc
     4381 tgtcgaaccg atcgtccagc caaataggta aagtcctgaa gttccttgag taccacgata
     4441 cgccatttta tgatctgtca agttatttcc cacaagcaaa ccttgacgaa cggcatttgt
     4501 tgccaaagga atatagtttt gcgtgccact tggattgtag ttgactactg cagagtctcc
     4561 agctgcaaaa atgtctggat tacttgtctg catgtattca ttcactttga ttgccccgtt
     4621 tggcagtgta tctacttttc cttcgatcag ttttgtattt ggacggaagc ccacacataa
     4681 aatgaccata tctgtatcaa atgtatctgt tggtgtagct acttgtttca cttttccttc
     4741 ttcatctgtg ataaattcag taacattttc gcctagcgca agagtaacac cgcggtctgt
     4801 caattctttt tctaagatat ctgtgaatgg tttatctaga tatttattca agatgcgatc
     4861 taatccatca atcaatgtca cttgttttcc agattcgaca aaagcttcta ctaattcaat
     4921 accgatatat ccaccaccaa caattactac acgttttgct tctttcgctt gtgcaataat
     4981 ttcatttgct tgattgtagt ttttgcatag gagtacattt ttcgcatcga ttccactaat
     5041 cggtgggata ataggccatg aaccagttgt catcactaat ttatcataag aaaccgtttc
     5101 tgtttctcct gttttcagat cttttgccgt tactgtttta ttttctgtat cgacgtctgt
     5161 tacatcgtgt tccattttaa ctgttgctcc taatgaagat aattcttcag gatttgagta
     5221 gaacaagcct gctgggtcct ttactactcc gccgacataa agtgcgatac cgcaagataa
     5281 gaaagaaata ttgtcatttc tttcatatac tgtcacttct gcttctggat gattttttaa
     5341 aatacttttt acagctgcag tacctgcatg agtacagcca attactacta ctttcattta
     5401 tggtccctcc gattaacatt ttgtccttcg acaaaactac tataacagaa ataaaacgga
     5461 tacaacagtt taatgcttgt gaattcacaa aggtttgttt gtagtttcat tccttttcaa
     5521 gcataagaac ccaatatcta caagattctt attttaataa tattcacttt tatatctaag
     5581 attgtgaata aaagacaaaa aaataaaaaa gctagcgttt tttttacaca gctagctttt
     5641 tttaggtata acacttgtag aaactgtttt tacatttgcg ccttttttgc tgactcttta
     5701 caatccggac agagtccata caattccaac cgatgttcat taatctcaaa atcagtcagt
     5761 ctgcttgcag ccatttctat atcttcaagc cctgggtaat gaaaatcaac aatcttaccg
     5821 cagttttcac aaattacatg ataatgacgc tttgaactga aatcaaatcg gctagaagca
     5881 tcgccgtact tcatttcttg aacaaatcca atcgaagtaa ataagcgaag gttattgtaa
     5941 acagtcgcaa cactcatatt tggaaaccga tgttcaagtg ctttataaat ctcatcagcc
     6001 gttggatggg aatgatgttc aatcaaatat tctaaaaccg cgtaccgttg aggtgtgatt
     6061 cgaatgttct ctttttttaa cttttcgata acctcttcga ctaacttatt ttccatgtaa
     6121 acaccccacg ttgttattct cgtcttatca tactattttt acattttaaa atccacttaa
     6181 catgaaaaga aaattcaatt ttattgtatt
//
LOCUS       DAHHPA010000125         6169 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.130, whole genome shotgun sequence.
ACCESSION   DAHHPA010000125 DAHHPA010000000
VERSION     DAHHPA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.130"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(129..776)
                     /locus_tag="NBH55_002543"
     CDS             complement(129..776)
                     /locus_tag="NBH55_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCD1510478.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1184..1687)
                     /locus_tag="NBH55_002544"
     CDS             complement(1184..1687)
                     /locus_tag="NBH55_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1510479.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1810..2574)
                     /locus_tag="NBH55_002545"
     CDS             complement(1810..2574)
                     /locus_tag="NBH55_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCD1510480.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2682..2957
                     /locus_tag="NBH55_002546"
     CDS             2682..2957
                     /locus_tag="NBH55_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCD1510481.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            complement(3158..3505)
                     /locus_tag="NBH55_002547"
     CDS             complement(3158..3505)
                     /locus_tag="NBH55_002547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510482.1"
                     /translation="MIPSVSFLFKKLKTFFILPEIEESNELTASDRSAFLLPVFNTWG
                     KAPQKQPYRRCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFH
                     NLIDGVLSAASRY"
     gene            3673..4602
                     /gene="yidC"
                     /locus_tag="NBH55_002548"
     CDS             3673..4602
                     /gene="yidC"
                     /locus_tag="NBH55_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCD1510483.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            4705..6053
                     /locus_tag="NBH55_002549"
                     /pseudo
     CDS             4705..6053
                     /locus_tag="NBH55_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         1925 a         1178 c         1131 g         1935 t
ORIGIN      
        1 aagaaagggg ctgtccccag actttagtag gcgtcattag ctatctttcg ttagcattta
       61 aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa
      121 caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg agccaatttc
      181 gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt tttcgtatac
      241 gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg aaacattcga tgttttctga
      301 cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat cccttttaat
      361 ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt ccagccgatg
      421 tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt tttcactttt
      481 cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctgaac gatttgcttc
      541 agctcctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa aactgtcgtt
      601 aaaaatgtat cgatttctgg cagttcgtga tctaatatgt ctcgaatctt ttcagctgct
      661 agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc atttaataac
      721 tgttctactt ctcttattat aaaaaaatat tggtatggtt ttattgtttg ttccatgatt
      781 ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa
      841 gtatgtgtta cctgaagatc ctggactctt ttaaataaga tacgttttgc atcttatcat
      901 cacccccttt aatgcttatt attctatgta catgattcat tcgataaata aaagtaaacc
      961 gtatcttgaa cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa agtcgtgtca
     1021 aagtcccttg tttcgaatga atggtgttca aaagcttgca tctgcagtaa taagccgaaa
     1081 cttcacaaaa atttgagaag taattttcgt aaattccttc ttatttcttg aagctgacca
     1141 cttctgtcac aacctcaata tggtctatta ttggttttac ctgttaaatc gaggattctt
     1201 tcccaaagta acgttgccat ctcttacgca tacttgcaga catgggctga gccgcttctt
     1261 tgactgcgca gtatttgtcc acgaatgtca caatatatcc ttctttatac ttaggcggtg
     1321 cgatcgttgc accccacatg tgcttcaaaa tgatatctcg ctcaagatcc gacaaatcgg
     1381 tgattttttc tgcattcttt acagcaattc ttggatggat atacgcatgt gtcccctcgt
     1441 caaatttcgt cgtacgccaa tcatagtaga acagatcatg caaaagtcca gcacgcgctg
     1501 ttgctttagc attgccaccc catttttttg cgagcagata actatagtaa gaaacactga
     1561 tggaatgctc caaacgagtg gaatgaacat gctgtgtgta attcgctaat tttttcactg
     1621 cttctgtttc taaaagatct tgtacataag acatatattc ttcgtcttca cgccattttt
     1681 ctgtcataat tatcgatctg ccaccttttt gaatttcatt ttcttctgaa attgatctaa
     1741 gtataccacc agtgttcgga ttattctagt cgctctagat aaaatagcta aagcttaata
     1801 gttccttaat cataaagaaa aatgataaag aacgatccct gctgcgactc cgacattcaa
     1861 tgattccgca tttcctttca ttgggatata tacgttctga tcagtcaaag acaagatttc
     1921 ctgactgact ccctgacctt cgtttcccat gattatagcg aaatcctctg ttttttctac
     1981 tttgtctaat gcatgtgctt cttcatttaa ttctgtaccg tacaccgtaa accctttgcc
     2041 tttcattaca gggattacat ctgccaaatc tttttgtaac acaggcaaat gataattgct
     2101 tccctgcatg cttcgcagca cctttgtact ataaatatct gccgttcctt tacctaataa
     2161 aacaccagcc ataccaaacg catctgccgt acggatcatc gttcccacat ttccaggatc
     2221 ctgtacatta tctagtaaaa gccagcctcc tgaaaaatct ggcgtatctt cagatgttaa
     2281 cgtcatcact gctaagatcc cttgcggtgt aggtagatct gacatagcgg tcatgacctc
     2341 atctgaaact agaaaagtag aaatctcttt gtttacagcc agccagtctc cccattcttt
     2401 catacctctc tgtgtcacaa aaagttcttt tatttctgca tcagacgacg cagcttcttc
     2461 gatcagatgg aacccttcca aaagatattc tgccttttct tctctgtatt tctttttatg
     2521 tagttttttt aattctttga ttcttggatt ttttgctgat tggatctctt tcacattcct
     2581 tcacctcacc tgattatatc atggaatagg tgcttgagct tgtttcatcc taaaaaaatc
     2641 ggtatcattg atttataaaa caggaaagaa aggtgacgaa catgcgcaaa gttaaaatga
     2701 atgtccaagg acgtgtccag ggcgtgggat ttcgctatat gaccaagatg gttgctgatc
     2761 agttaggcgt gaccggaacg gtaaaaaacg aagacgacgg ctcagtcaca atcgaagcaa
     2821 tcggaaatga cgacatcatc caaaaattta tcgaagaagt caagaagtct ccgagcccaa
     2881 gtggacgagt gcagtatgtg gatgtccaag aagatccgat gattgaggaa cgaaagaaat
     2941 ttgatgtggt gggataaggt tgtgaaaagg tcgcttagtt ttgagtatta aggaaaattt
     3001 tgtgaaaatg gctttccaca tttttgcata aatttgactt aataccgaga aactgacttt
     3061 tgaacaccgt cgataaggtt gtttttgaag cgctttgcct cgagtattaa ggagaaacat
     3121 tttcaaaata gcttctcata ttttgaaatg taatgactta ataccgagag gcagctgata
     3181 aaacaccgtc aataaggttg tgaaaaagct ggtctgcatc aagcattaag gactggttgt
     3241 gaaaaagtcg cttagttttg agtattaagg aaaattttgt gaaaatggct ttccacattt
     3301 ttgcataaat ttgtcttaat accaagaaac tgacttttga acaccgtcga taaggttgtt
     3361 tttgaggcgc tttgccccaa gtattaaaga caggtagtaa aaaagctgat ctatcacttg
     3421 cagtgagttc atttgactcc tctatttcgg gcagaataaa aaaagttttt agttttttaa
     3481 ataagaaaga aacacttgga atcactcttt tctttccaag tatttctttc ttgatttttt
     3541 gtcttttttc aaaatttttc gctacgaaaa agaaagaata tttatatgaa atttctatga
     3601 agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag
     3661 aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc
     3721 ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcagga
     3781 atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgagaat
     3841 ttcaattggt cttacggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc
     3901 ttgccactag gtatcagtca atcaaaaaaa acaatgatcc aatctgaaaa aatgcaagcg
     3961 ttaaaaccac aagttgaagc agcacaacaa aaactaaaag ctgcaacaac acgtgaagaa
     4021 caaatggcag cacaagcaga aatgcaacaa gtatatcgcg aaaacggatt gagtatgacc
     4081 ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtatttt
     4141 acggctcgct acacagaagg tatccgtgaa tcatcttttt acggcatcga tctaggctca
     4201 ccaagtatgg tgcttgttgc cattgcaggg gtcgcttatc tgcttcaagg atatatttca
     4261 acaatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct
     4321 gctatgatcg ttttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtg
     4381 ggtggtgtgt tcagttgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg
     4441 aaagcacaag tggcagaaga gttgaaacaa aatccgccaa aacaagtcgt cactccaaga
     4501 aaagacgtga cagaatcggc aaagccagct gacaataaac aaccaaaaaa cagtacaaac
     4561 aacaaaggcc gcaacgcagg aaagcagcaa aagcggaaat aaaaatatat gacaaaaaac
     4621 tgtagacaaa taaaaaaatt tctttgttct acagtttttt tgttacaatc aaagaaaacg
     4681 tcgcttttcg aggaggaatg tataatgatg tccaaataat caatggatag tcgaatggaa
     4741 gaagccaaaa tttcgctaga tgcacttgtt cctaaaaatc atttggtcca taaaattgac
     4801 aaaatattgt tatttgattt tatttatcca attgttgaat caacttactc gaccattggt
     4861 cgtcctagcg ttgatcctgt catcttagta aaattagtat tcattcaata tttatttagg
     4921 attcgttcga tgcgtcagtc cattaaagaa gctgaaacaa acctagctta tcgttggttt
     4981 cttgggctta gtttaatcga taaaattcct catttttcaa cgtttggtaa aaactatgta
     5041 cggcatttca gagaaaccga ttgtttgaac agatatttat cattatcctt gaacaagcag
     5101 ttgaagctag atttattcat gaagaaattt tgtatatgga ttcaacctat attaaagcga
     5161 atgcgaaaaa acatattttt acaagagaaa tggtacataa ataagccatt ttataccaaa
     5221 aacaattgaa agaaaaaatt aatgaagcaa gaattgcaga aggcaaacgt ccttttggtt
     5281 caatcattca tcaggaggtg gtagaaggga aaattagtgt cgcagatcca gaaagtggtt
     5341 attatgtaaa aacagaacaa gaaaaacagt ttgcttattc tgctcatacc gtttgtgatg
     5401 aaaatggttt tgtattagat gtcatgatta cgcctggaaa tctacatgac agccgaatgc
     5461 tcgtgccaca aatcgaacgt gttaaatcat gttgtggtgt agctgctgat gctgcgtaca
     5521 aaacaccttg gaacgccaag tatttaatcg atcgaaaact acgacccatt tttccttata
     5581 ctcgacccaa aagaagcaaa gaacgtttta aaaagaaaga tttttattat gatccctatt
     5641 ataactggta cctatgccca aatgatcaaa cacttcagtt tcgcacaact actcgagatg
     5701 gttataaaaa atatgtatct aaatctttta tttgtgaaag ttgtctatta ttgaaaaatt
     5761 atacagaaag ccagaaacac cagaaagaaa tacatcgaca tatttaggaa gactacttag
     5821 atgaagcaga acatttacgc catacggtgt acaatcgcga aaaatataaa aaaaagaaaa
     5881 gaaacgattg aacgcgtttt tacagacgca aaggaaaagc atggaatgcg ctacaccaaa
     5941 tatcgaggct tggaaaaaac caggtgcata cgatgcttgt ttttgccgca atgaatctgc
     6001 aaaaattagc catttggcag tgggggcata tgtctttata cccgtggtgc tagttaattt
     6061 tttcctagag gaaaatgtct aaacatgttg gtaaatcaac aaaaaaacgc atatagtagt
     6121 ctcaaaactc ccaagaaatg aggactacat atgcgttcat caaaatata
//
LOCUS       DAHHPA010000126         5915 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.131, whole genome shotgun sequence.
ACCESSION   DAHHPA010000126 DAHHPA010000000
VERSION     DAHHPA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.131"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(141..599)
                     /locus_tag="NBH55_002550"
     CDS             complement(141..599)
                     /locus_tag="NBH55_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1510484.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(612..1943)
                     /locus_tag="NBH55_002551"
     CDS             complement(612..1943)
                     /locus_tag="NBH55_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCD1510485.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1954..3396)
                     /locus_tag="NBH55_002552"
     CDS             complement(1954..3396)
                     /locus_tag="NBH55_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1510486.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3383..4147)
                     /locus_tag="NBH55_002553"
     CDS             complement(3383..4147)
                     /locus_tag="NBH55_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCD1510487.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4251..5126)
                     /locus_tag="NBH55_002554"
     CDS             complement(4251..5126)
                     /locus_tag="NBH55_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD1510488.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5381..5465)
                     /locus_tag="NBH55_002555"
     tRNA            complement(5381..5465)
                     /locus_tag="NBH55_002555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5429..5431),aa:Leu,seq:caa)
     gene            complement(5483..5554)
                     /locus_tag="NBH55_002556"
     tRNA            complement(5483..5554)
                     /locus_tag="NBH55_002556"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5520..5522),aa:Gln,seq:ttg)
     gene            complement(5564..5636)
                     /locus_tag="NBH55_002557"
     tRNA            complement(5564..5636)
                     /locus_tag="NBH55_002557"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5601..5603),aa:His,seq:gtg)
     gene            complement(5698..5782)
                     /locus_tag="NBH55_002558"
     tRNA            complement(5698..5782)
                     /locus_tag="NBH55_002558"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5746..5748),aa:Leu,seq:caa)
     gene            complement(5803..5873)
                     /locus_tag="NBH55_002559"
     tRNA            complement(5803..5873)
                     /locus_tag="NBH55_002559"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5839..5841),aa:Cys,seq:gca)
BASE COUNT         1813 a         1257 c          901 g         1944 t
ORIGIN      
        1 taaatcatag gagcaacaaa aaaattcgat aattaacaaa aatcaaaatg attaaatcta
       61 gtgattgaaa catatcattt caaaaaaatt ttgtaaattc acccataaat atcaccctat
      121 ttaacttttt tcatcgtaca ctaaaattca aaacaactat ctttaatctt tacttcttgg
      181 ttttgtttta ataaatttcc ttcaaaaatg cctggagaaa ctacaatgat caccgtatct
      241 ttttgtgctt gctccaactt cttcaaatct ataatagcta acagtgtccc ttcttttacc
      301 tcctggttac tttcaacccg tatctgaaac ggacttcctt cgagttcaac tgtatctaaa
      361 cccatatgga tcagtatttt tctgccagat tcactttcga tcgttatggc atgtcgggta
      421 ggaaatatat tatcgattat tcctttaact ggcgcatgga tatatccatc atgattcgtt
      481 atagcgtatc caactcctat catttttgaa gaaaagacct cgtcttttac tgctgttaat
      541 ggaataattt gaccactagc tacagctttt agaatgtttc ttgttttaaa gaaatgcact
      601 ttttatcacc tctactttaa tgctggccaa tattttttgt ttactttgat catttcatct
      661 agtatttttt ttgcgactct ggcatttggt acggtttgat tcaatgtaaa tgcttgtaaa
      721 gctttttgat aagaaccttc aaagtaagca tctactagta acttctctac tgcaacttgt
      781 gcctccatca atcctttatg aaaatcactg attggttttc ttagtgtaat tggctccaca
      841 ccagttgcac caacatatgc cggtatttca acaactgcat ctgaccgtag attcggaatc
      901 gccccttgat taggaacgat tagcataaag cgacgacgat catcattcaa caaggaaata
      961 gctatatcga cgatatattg tccatgctcc ccaaaattaa agttcaatac ttcttccaca
     1021 ataccgcggc gtattttatc agccatttct ttggtatttt tctctcgtcc atccatgacc
     1081 atattagcac gtgtatactc tttgtcagta tgctcaacga ccatatcagg atataagtaa
     1141 tactctaagt aattattcgg gatattactt ggaaaatttt tcgtgatgac tgacatcatt
     1201 tgaaaggttt tttgccaagt tttatctcct atattaaaat cggcgacttg catttctttt
     1261 gttattagtt tttcaataat ctctggcata atttctcgtt gcaattcttt atcataaatc
     1321 gatgtatacc agccaaagtg gtttaaacca taatacgtaa cgatccaatt cttacgatca
     1381 taaccgtaat tcactgcaat cgtttcttca attgaaatag tcatatcaca ggcattaatc
     1441 atttttacat tcggatattt acgacgaaca gcttcagaaa cgatcgactc tggatttgta
     1501 taattcaaaa tccatgcttt gggggcatag tcttgtatat gatcgacaat ttccaataaa
     1561 cctttgattg agcgcatccc ataagcaaat cctccgagtc cacacgtttc ttgtcctact
     1621 aatccatgtt taagtggaat cttttcgtcc ttttctcgca tctccaatcc tccaacacga
     1681 atttgtgaga aaacaaaatc gcatcctgta aaagcaagct ttggatcatc cgttgcacga
     1741 atgcgaatat ttgaaaaacc ttttcttttc agcataaatt taacaatcaa gtacatatcc
     1801 atatttcttt cttcatcaat atcatataaa cgtatttcag ataatggcaa ccgtttttca
     1861 ttgttcaaaa tggcttgaac gattcctggt gtatacgtac tcccgcctcc agctatagtg
     1921 ataatctgtt ttttcatcat catcttttcc tttctattct tctaataatt tattgaattc
     1981 tgccataaca taagtcactt ctaagccgat gataatttga taattttttt cagttggttt
     2041 aatgatcccg cttatcccta attgcttgat tcgctgttcc tcgaccatgg tgggatccac
     2101 tacctctacc cgtaggcgtg tattacaact atagacatcc aggatattgg cttttcctcc
     2161 aaacgcttgt agcatttttg ttgccatata ttcataattt ttacttgcta accctaactc
     2221 tgtttcttct aaggttactt cattgccaaa ctctgcctta gcttcacgtc ctaatgtctg
     2281 aatgtttctc ttattgatca aataataaaa agtaacgtaa tacaaggcaa aaaagaaaat
     2341 tccgattgga agaatcaaaa tagcattggt agcgattcga aaattgatga gataatcaac
     2401 aatacatgct ccgaatgtaa aaccaattcg tatattgaat aagtaacaga caatccctgc
     2461 taaaccggca aaaagtgcat ggatcacgta caacatagga gaaagaaaca tgaaactaaa
     2521 ttcaacaggc tcagtgatat ttgcaataaa tgaagttcct gcaccgcttc ccatgattcc
     2581 tttgaccatc tctttatttt ctttataagc tgctttatag atagctagac atatagctgg
     2641 tataccaaac atcattacta caaaaaaaac tgataaaaac agtccagcct gaggatctcc
     2701 gttgataaag cgtgtcatct ctcctctgaa aacttccttg ctaggagatg tatagcttcc
     2761 taaatcgtaa taaatatacg tattcaacac atggtgcagt ccaaagggga tcagcaaacg
     2821 attcaaaaac ataaatatcc ccacacctaa tgctcccata tcaacgagca gttttgcaaa
     2881 attatcaatc ccaatttgga tgagcggcca gataaagccc aaaaaaacag ctgcgattgt
     2941 ttgtagaatc atcaccatag tcaatgggaa tttttcgccc gcaaaataag aaaaaactac
     3001 tggtaatttt tgttctttaa agcgattata cgtccatgcc gctactaaac cggaaataat
     3061 tccaccaaaa atccccatat tcacttctgg gtccataatt tctaagcctt ttttcaaggt
     3121 agcaatcgct aaaaaaccag taagtgcagg tattcctttg tccttatttt tgacaaaacc
     3181 gattgcaact cctatagcaa ataagatatc caagtttcca aaaacggcat ttccagctgc
     3241 accaattact gggatgttca gcatatcgtc agctgataaa cgcatgatca atcccgctaa
     3301 tggcatcgct gcaactggaa gtagcatcgc tcttccaagc ttctgcacat ttgatttaac
     3361 attcattttt attcctcctc tattaaatat cctcatatgt tactaactca atcaaaggat
     3421 catttttcac tttttgagca aatgctgaat gaattaaaaa ttcgacttgg tgggtacgag
     3481 gatacacaaa acttgaacca tttaacaatt cctgatccaa atacgctgcg tgacacatga
     3541 cttcgatcgt tttatcttct ctcaatatca tgagtaaact tgccaaatat tcttccacac
     3601 tcgtattgat ttgtcgtttt ttttcatcag tttctcctac gacatcgaaa caaggctcaa
     3661 aataatcgac atgacgaact tcatcttttc gttcaaagtt gctccgaacc ggcaaatcat
     3721 atttttttgc taatgtaaca actacctcct gattttcatc caaagtatga gtatgatgat
     3781 gactgtctaa atgagttggt atgatccccg catgataaat tttttgaatc tgagcattcc
     3841 attcctgata cagttgatct ttgtcaatgt taaatggatg ctgataataa gataaggatc
     3901 tgaactctcc gctttcgata agagtatcga cagttttgag gagtggtttg ccacacgtca
     3961 atgttaaatg gactcctaca cctagcctag gcatttcctt tcttagtttc acagcatgct
     4021 cgaaacccgg catatttgcc atcaatgtag ttgatgtcaa aattcctcgc tgataagcat
     4081 ccataatccc ataattcaca ccatggctat agccaaaatc atccgaatta aaaatcactt
     4141 ttcccatttc attctccttc ttttttctac taaaataggc gcaaccaaat aatcatagtc
     4201 catttactat gtgtcatttg gttgcgccta tagatagtta cgcccttatt ttaatagatt
     4261 cttgctagat gaattgccaa atagatgatt tcattttctt gaatggcaag ttgaaattgc
     4321 tgttcgatga tatctttaat ctttagtgct gtacaatacg ctttgctatg tttaaatgtt
     4381 tcagtaaaaa aaccaatatt ttctactggc gtttcttcct gataaatcaa gcgtttaacc
     4441 aggaatttga tatgaaccat taagcgatga taagtcagtg attctttatc ataaacgttt
     4501 gaataggatt tttctagatg ggacaaaatc gtggtcgtca tttctactat ttttaagctc
     4561 tttaaactat taggctctcc tagctcagca ttaatgatat gcatagtgat gaatcctact
     4621 tcatttgacc ctaactcggt ttgatatcga tgattgattc gctcgaccac ttctactgct
     4681 atatcatatt catttggata aaggaatctc atttctggca acataattgc tgtgacatct
     4741 ctatcttctc tttttcgttg aatagcaaaa taaatatgat ctgtcaatgt aatatagata
     4801 tttgaatcta atggagagga gatcctagct gttatcagtt caatcacttc ttccgcaaaa
     4861 tccataaatt cgattggaat ctgcgagact atctcactga atcgtttgat cttttcttta
     4921 gaatctaata caaataattt gtcagcatcc tcaacatcaa atggcttact cactttagca
     4981 gaaaagccaa tccctttccc catcgcaatt ttttctattc catcgtcatc gatcaataca
     5041 gcattatgat tcagcttttt catcacatac accgttaatt cacctcctct atatgataaa
     5101 acaaaacaac ttgcagactc cttcatacat atttttgtct ccaaacaatt tttaaagaca
     5161 aaaaacgacc taagaaagaa ccctttctta ggtcgtgcct aattgaatag tagcacccta
     5221 cataatttac aaaaatagtt taacacataa taaaagcgtt atcaatatct tttttactcg
     5281 ttttatgata atacttgtat cgaattgatt tggaggtaat tacataaaaa caaaaaaaat
     5341 atagcaactc tctataagaa agttgctata ttgataatta taccggcggc cggggtcgaa
     5401 ccggcacgtc ctcaacggac actggatttt gagtccagcg cgtctgccaa ttccgccacg
     5461 ccggcaaatt taaaaataat aactggggta gctggattcg aaccaacgca tgagggagtc
     5521 aaagtccctt gccttaccgc ttggctatac cccaagaata aagggcgaat gatgggaatc
     5581 gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac attcgccatc
     5641 ataaaagatg ttgtttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac
     5701 cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt
     5761 ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg
     5821 aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac
     5881 tattatcaat ctataaaata aagaataaat tatag
//
LOCUS       DAHHPA010000127         5911 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.132, whole genome shotgun sequence.
ACCESSION   DAHHPA010000127 DAHHPA010000000
VERSION     DAHHPA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.132"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(368..1486)
                     /locus_tag="NBH55_002560"
     CDS             complement(368..1486)
                     /locus_tag="NBH55_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510489.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="NBH55_002561"
     CDS             1957..3264
                     /locus_tag="NBH55_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCD1510490.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="NBH55_002562"
     CDS             3383..4582
                     /locus_tag="NBH55_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCD1510491.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="NBH55_002563"
     CDS             complement(4609..5877)
                     /locus_tag="NBH55_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCD1510492.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1815 a         1252 c         1058 g         1786 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact a
//
LOCUS       DAHHPA010000128         5691 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.133, whole genome shotgun sequence.
ACCESSION   DAHHPA010000128 DAHHPA010000000
VERSION     DAHHPA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.133"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            130..468
                     /locus_tag="NBH55_002564"
     CDS             130..468
                     /locus_tag="NBH55_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCD1510493.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            455..634
                     /locus_tag="NBH55_002565"
     CDS             455..634
                     /locus_tag="NBH55_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510494.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(677..1147)
                     /locus_tag="NBH55_002566"
     CDS             complement(677..1147)
                     /locus_tag="NBH55_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510495.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1234..1932
                     /locus_tag="NBH55_002567"
     CDS             1234..1932
                     /locus_tag="NBH55_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCD1510496.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2110..2451
                     /locus_tag="NBH55_002568"
     CDS             2110..2451
                     /locus_tag="NBH55_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510497.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2444..3115
                     /locus_tag="NBH55_002569"
     CDS             2444..3115
                     /locus_tag="NBH55_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCD1510498.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3121..3807
                     /locus_tag="NBH55_002570"
     CDS             3121..3807
                     /locus_tag="NBH55_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510499.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            3810..4559
                     /locus_tag="NBH55_002571"
     CDS             3810..4559
                     /locus_tag="NBH55_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510500.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            4571..4840
                     /locus_tag="NBH55_002572"
     CDS             4571..4840
                     /locus_tag="NBH55_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510501.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            4845..5009
                     /locus_tag="NBH55_002573"
     CDS             4845..5009
                     /locus_tag="NBH55_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510502.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            5002..5304
                     /locus_tag="NBH55_002574"
     CDS             5002..5304
                     /locus_tag="NBH55_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510503.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            5301..5462
                     /locus_tag="NBH55_002575"
     CDS             5301..5462
                     /locus_tag="NBH55_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCD1510504.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            5459..>5691
                     /locus_tag="NBH55_002576"
     CDS             5459..>5691
                     /locus_tag="NBH55_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510505.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKN"
BASE COUNT         2130 a          877 c         1204 g         1480 t
ORIGIN      
        1 gctgaaagat ttggaattag caaaagctac ttatctttaa ttttaaacgg taaaaagact
       61 tctgcagaag caaacagaat tatagattcg attatcacta tgtacgaatt gtaaagggag
      121 gaacagctaa tgcaatatct agaagcaaaa atcccaattc cagaaggcta tgtaattatc
      181 tcccaagtgg attatgagga gttaaaaaaa gctgatgata ctggtagatg gatgacgttg
      241 ccagaagtgc tagaacggat taacagaaaa tatgattggt ttacttctag agttttaaag
      301 aacccaagat atagaaatat tatcgatatc gagaaaaaca aaaatggatt tgtctattat
      361 ccagttgaag gaagagacac atatctattt ttaagaagta aaacacttga atttttagaa
      421 acaaattttt cggaaatctt aaggaggcaa gcggatggga aaatttaata gagcgctagt
      481 gttcagcgca ccgctaatca tctacgcttt aggactttgg ggaagcaggc aagcgttgat
      541 aggaacgatt gtttacatgg tttggatttt tatggggctt gatgaagctg aagctgagta
      601 cagagcgaaa aagccaaccg agggggctga ctaaaaatga atagaaaaga gaaactagaa
      661 tgtatattac tattactcag tttaattctt tcactaattt ctctattggg aagtttgtat
      721 ttttgatatc aacaattttg tggatttggg ttctatttag agtaaatatc atttttgttt
      781 ggtgattaag gagtttatta aaattaattt tccctacttc aaatgcaagg taacaagata
      841 tagcactttt aggaggaata tttagcggta cagcttcaga gtatattcta ttatcatcga
      901 ctgatcctcc tttagcaatt aatttcttaa atggagtagc tgaccatctg cggtttaagt
      961 tagcggatga aagttctaaa ttaactaaca tagctggttc tgttgaatag ttagaaatga
     1021 ttacttttgt ataaatcata tctgcagcga agtaagaagc attaagttca acttctagtt
     1081 gagggcgcat tccttttatt tttatccctg ttaaaaacgt actcaatata aaacttatta
     1141 ttgacatcca ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc accagttttt
     1201 aactaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat tttgaaaaat
     1261 aaagaagctg tgactacgag cttacaagtg gcagaaagct tcgagaaaaa acatcaacat
     1321 gttttaagag atattgatgc actaaaaaaa gatgtgtcca attttggaca gatgtttgta
     1381 gaaggtaatg aaccagattc atatggcaga aatcgacgag ttttcttcat tagtagagat
     1441 ggatttttct tgctggctat ggggtttaca ggaaagaaag ctatctactt caaacaaaaa
     1501 tacattgaag cattcaacga aatggaagat gttattcgca agaatactgt tcctcaaaca
     1561 attgaagata tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc
     1621 atgcttaaag atactatgcg aattagcgga caacaagagt ttgaaattaa gcaaaaagga
     1681 aatatgaaag ttatgggagt tctaggggga aaagaaagcc gagcttatga agaaatcagc
     1741 aaaaaagtat tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat cccaagatat
     1801 ggcgagttac ctcgtaagag attcgatgat gctgtttcat ttattgaaat gtggttgcca
     1861 gaaactgcga tccgtatgga aattgatcaa ctgaacagac aacagagact ttttggtgat
     1921 gacaatgaat agagctgaag cgctaagaat agggacggca attgctaatt gctggtggaa
     1981 atactataaa ccaatcatcc taagccaaca acatattgac aagcaaaaag catggcaaca
     2041 aataaaaaag tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga
     2101 ttttagcata tggaaaaaga actttccact ctagatcaat atttgattga tcctgattgg
     2161 ggcaagccga aaattgagga aacaagtggt cgaaaaatca gacgaaatct tttgacgaat
     2221 gaagaactag cttgggatca agatgattta ggcaaccatg taactatttg ggatcatgtt
     2281 tatcttatcc atctatcgaa gcattcgaat aaacctgaat atatttacgt catcgaagat
     2341 ggcttgattg atgcgctaga agagtacgac agagataact tgattgatat ctcttattac
     2401 ggaccaggta agaaatacat tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg
     2461 gacaacgaac tttgaaaaac tttttagtcg taagttaaat aaaattctca agaaaaaagg
     2521 aaattttgat tatttatctt gggctcacgc gtgggagatt atgaaaaaga atgatccaca
     2581 ggcaacggta actattaatg agtacaaaca ctacagggtt gtttctggaa ctcatcaaga
     2641 ctttcttgtt gaggaatata aaccttttct tatggatgaa actgggactt atgtatctgt
     2701 ctcagtaacg gttaaaggac acacggaaac cgaattattt cctgttttag attatcgaaa
     2761 ccaaccagtt gttaaaccaa atgcaatgca aatcaataac tcattgaagc gatgctttgt
     2821 gaaagcattg gctctacacg gactgggatt atatgtattt caaggggaag atattccaac
     2881 accacctaga atcgatacaa agaaattaaa catgctagag acgattctag aagctttcaa
     2941 tgagcagatg ggtaaagata tgaccaaaac cttaatcgaa tatgttaatg agcagacaga
     3001 taaattaggg ctcttagctg ataacgttga aactattgaa cagttaagct atgagcaatg
     3061 tgccttgatg gagcgagcaa tagcagctaa gagaaaagaa ttagataaga agtgatatga
     3121 gtgtttaaac cattaatcga ttcatattca gcggttctga aaaagttcaa aggaaaagac
     3181 ataagcgcaa ccatcaatga ggaagtgaac attgatcgac taaagacgat gtatgacggc
     3241 tacgatggtg atcgagtcat tgaaattcgt tttattgatc ctagacgttt caccgtacag
     3301 caacgaaact tcatctatgc gctgataggc gatattttta tcgatacagg catgccaacg
     3361 gacttctgga aggaattctt ctactttcgt tttgaaggtg tcacagggcg caaaataagc
     3421 ctgaaagacg aatcgaatac aactgtgagt gatgccaatg tcttagcaaa tatcatctta
     3481 gatttcatct ttgaacatca tattcctttc aaagaaggct atgagatttt acctgcgaat
     3541 caagaatatt acttctacaa atgcatcaca aaaagagtct gctgcatctg tggcaaaaca
     3601 ggagctgaca tcgatcactt tgacaaagcg ctaggaagac gaaagcgcaa agaagttgat
     3661 catgcagagt acacatttgc agcactctgc agaatccatc acacagagaa gcacaaaata
     3721 ggtgtgatta atttcaaaaa taaatatcaa atcaaaggga tcaaattaaa ccagaaaaca
     3781 atcaaaaagt taaatattgg agggtaaaaa tgacagaaca tcgaagttat tatgcgatta
     3841 taccagccaa cgtaaggtac gacaaaagac ttaaaccaaa tactaagttg ttatacggag
     3901 agataacggc cttgtgtaat gaaagaggct tttgttgggc aggcaatgag tactttgcag
     3961 atttatatgg tgtgaataaa gagaccatat cgcgatgggt aagtgatttg attaagtttg
     4021 gatacttgaa tcgggaaatc atttacaaag agggtaccaa tcaaataatc aataggtacc
     4081 tacgaattaa tcaataccct attgacgaaa aacgcaatac ccctattgac gaaaaagtca
     4141 aagataataa tacatctatt aataatacat ttaataatac aaaagaatat ataagagagt
     4201 taccgccttc gaaaaaatcg aaggctaagc ccgtccgtca taaatacgga gagtataaaa
     4261 atgttctttt gtcagatgag cagatggaga aactcaaaac agaattccct aatgattacc
     4321 aagagcgaat cgaacgactg tcagagtatt gtgaatcatc tggtaagact tataaaaact
     4381 atttggcaac tattcgaagt tgggcaagga aagaaaaaag tgaacctaag aacgcaagca
     4441 gtggatacaa gcgcacagga agacgagaga agcttcctga atgggcaatc gaccaagaag
     4501 cctatctcaa gaaaaaagcg ctagaacgag ctaatagaca atcaaaagca ccattctaag
     4561 aggtggaaaa atgaagatcg attatctaga actaattaat gaaatagcga attataaaaa
     4621 gggcgaggaa ttagacgtcc tgagagacgt atatgatcaa ctcgaagaag ctggaattga
     4681 aggaattaag aatgatcgtt cgagttggag taagctcaga tactatttcg cactttatat
     4741 cgatacaaca caattaagaa atttagcata taccaaatta ctatttgttg attgtgttaa
     4801 aggcttgcaa aaacatctta atgaacttga gcaggtgtaa tcagatggac ctaaagacat
     4861 ttacagcaca gattgaacta atgcatcaag aagctttaag acaaagtgta tcgtacgaag
     4921 acaagtggct caacacgttc catggcggac gtgagagcgc acttgatcaa gtactcaaat
     4981 tactgaaagg agaatgtcgg gatggataag aaagcggcaa tgcagcgaat tatcgaattg
     5041 acttattcag aagattggca aaatgacaaa gaagctgctt cagaagtgat gaggcttgga
     5101 agagcgatgt gggcagacaa gagcaacaag ccaagaccac gaaaaatcgc aatttggcac
     5161 ggtgacaaac ttctagtgat agggacagct gaacagttag caagtctcac aggcttgcac
     5221 gagaaaatcg tgaggaaaag agcaaggtgt ggctacacag acgctaagaa gagaacgttt
     5281 agatacgtgg aggaatcatc atgacaacag aagaagtgat tcaaatgcgt attcgaagcc
     5341 ttcagcgtga gattgacgat ctggaacgaa caaaggcagt gatggtcaat gaaacggcga
     5401 gaaaggcaat cgatttgcac atagagaatt taagaaggga aatccatcga ttggaggaat
     5461 gagcgtggat aagaaagcag caatgaaaag aattgctgaa ttaaccaagt cagaatcttg
     5521 gcaagaagac aaagaaatag ttgcagaagt ccaaaagctc ggtaaatcaa tgtggactga
     5581 aaagcccaaa cggagaacgc cgagaaaaat tgcaatctgg catgatgacc gaattttagt
     5641 aacaggtact gctgaacagt tatctgaaat tactggatta agcaaaaaca t
//
LOCUS       DAHHPA010000129         5625 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.134, whole genome shotgun sequence.
ACCESSION   DAHHPA010000129 DAHHPA010000000
VERSION     DAHHPA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.134"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(237..740)
                     /locus_tag="NBH55_002577"
     CDS             complement(237..740)
                     /locus_tag="NBH55_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCD1510506.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1099..2124
                     /locus_tag="NBH55_002578"
     CDS             1099..2124
                     /locus_tag="NBH55_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD1510507.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2447..3325
                     /locus_tag="NBH55_002579"
     CDS             2447..3325
                     /locus_tag="NBH55_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCD1510508.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3801..3917
                     /locus_tag="NBH55_002580"
     CDS             3801..3917
                     /locus_tag="NBH55_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCD1510509.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4845..5183
                     /locus_tag="NBH55_002581"
     CDS             4845..5183
                     /locus_tag="NBH55_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510510.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5277..5507
                     /locus_tag="NBH55_002582"
     CDS             5277..5507
                     /locus_tag="NBH55_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510511.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1893 a          845 c         1041 g         1846 t
ORIGIN      
        1 gattgagata ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc
       61 ctttttgtta aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc
      121 aataatatag tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag
      181 accgtcatag atacacattg gaatgtgtct attacggtct ttttttacga acgattttat
      241 tgaagacgga cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg
      301 tatcccaaaa taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt
      361 tgataggccc ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc
      421 gctacgccaa taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata
      481 atccctaatt ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa
      541 ggaaaaaaga caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa
      601 aaagtagaaa aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga
      661 ttaaatgata gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa
      721 aattttgata cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg
      781 ctgaaaaatc ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta
      841 gaagcttttt tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg
      901 gaaaagttgc tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt
      961 tctccaagtt gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata
     1021 ctaggtaatg ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta
     1081 ctaggaagga gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat
     1141 ccttttcgta tcatgaatct gatgctactt atctttattg tattatggtt aggttacata
     1201 aatcatgatt ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat
     1261 caaaacatac cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg
     1321 gttgctttcg ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta
     1381 gctttggttg ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt
     1441 ggcttgtttt ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa
     1501 ttaccgatcg tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg
     1561 attacaaaat tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa
     1621 agatttcacg aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt
     1681 ctttccgttt atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca
     1741 tgtgcatcga ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat
     1801 ctgattggat cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg
     1861 cagttgcaga ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct
     1921 agaaattatg cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt
     1981 attcgtggag catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga
     2041 agtttcatcg gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg
     2101 gttgttcgtc aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa
     2161 gatttttgtc acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca
     2221 aattcacaca aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa
     2281 aattttgttt atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa
     2341 agtcttacag atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa
     2401 agtatgttta taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga
     2461 tatgaaaagg ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac
     2521 tttaactctt cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga
     2581 tgaggtgttt gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc
     2641 aaagaaaata gacaaagtct atgaagagga accttttgca gcattagcta tttatctata
     2701 tgcagcttct caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta
     2761 tttggcaaca atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag
     2821 ctatcgcttt gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat
     2881 tggtgaattg gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga
     2941 aggaaagcag tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat
     3001 aagagatagt tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta
     3061 ccaagtgatg tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa
     3121 agagtcaaaa atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga
     3181 cgatagtgga gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga
     3241 aagtacgagt ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc
     3301 tggcttaggt attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag
     3361 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
     3421 gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt
     3481 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga
     3541 acacacagta cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca
     3601 agagggcctt ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag
     3661 tcaatcagct agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac
     3721 catttaaaac acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa
     3781 gaagaaagtg ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga
     3841 aaagcccaaa tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga
     3901 atagtgggaa aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt
     3961 cggctatttt caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc
     4021 catttttaac atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa
     4081 ttccagttat atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct
     4141 gtagttattt ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta
     4201 ccttttaaca cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac
     4261 ataatcaaag gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg
     4321 ttgcaaataa ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt
     4381 cagctcactc catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc
     4441 gaaaaagtta actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt
     4501 actattgcta gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa
     4561 gttgtgtatt ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat
     4621 cactattaca agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta
     4681 aatgaatagt tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt
     4741 tcaatttgtg attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga
     4801 ttgtagtgtt tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt
     4861 caagtgagga acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa
     4921 atattgttcc ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa
     4981 taggatggta tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata
     5041 cagtaggctt agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata
     5101 ctgatgaaag tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata
     5161 aaaagtatat attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga
     5221 aaatttacta gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc
     5281 tggtagaaaa gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg
     5341 aaaatgagtg gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag
     5401 tttacagaga aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt
     5461 tagctgaagt agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt
     5521 atgaaaaatt ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta
     5581 ctaactttaa tgcagtataa agagtatcca gagtatttta tttta
//
LOCUS       DAHHPA010000130         5582 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.135, whole genome shotgun sequence.
ACCESSION   DAHHPA010000130 DAHHPA010000000
VERSION     DAHHPA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.135"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..971)
                     /locus_tag="NBH55_002583"
     CDS             complement(<1..971)
                     /locus_tag="NBH55_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510512.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYP"
     gene            complement(1183..2079)
                     /locus_tag="NBH55_002584"
     CDS             complement(1183..2079)
                     /locus_tag="NBH55_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD1510513.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(2072..2752)
                     /locus_tag="NBH55_002585"
     CDS             complement(2072..2752)
                     /locus_tag="NBH55_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1510514.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(2753..3535)
                     /locus_tag="NBH55_002586"
     CDS             complement(2753..3535)
                     /locus_tag="NBH55_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1510515.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(3528..4445)
                     /locus_tag="NBH55_002587"
     CDS             complement(3528..4445)
                     /locus_tag="NBH55_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1510516.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(4609..4932)
                     /locus_tag="NBH55_002588"
     CDS             complement(4609..4932)
                     /locus_tag="NBH55_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510517.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1649 a         1148 c          928 g         1857 t
ORIGIN      
        1 ggataaaatt catttaattt aattcttact aactcttgta gctcaacacc catatctaaa
       61 taggaaagct tcgtttgatc taagtcgtga ttcatagcat ttttttcaat cgaagtaatg
      121 tgaatgtcaa cataagcttg ttcatctatt ggcacaataa ttgaaaatgc tttttcaatc
      181 attttgatta aaataagtgt attctttgag atttcttctt cagaattatt actacgtgat
      241 aagaaattgc ttgtttcaat cctttctatc gcaatagcaa ggtggatagc taaagtttga
      301 aattcataat ctgtaaattt caattgtgtc ttattcatgt atcgattaag caattcggct
      361 acttttttca atatgtcctt atttaagaaa ttattcgctt caccaatgaa attgatattc
      421 aagtctaaga gatctgtttt actgttagat gctgtaaagc caccccaata atagttcaca
      481 acttctgaca tcaaccttct tttacttttt tcatcagctt tgatgcgcac gccatttcga
      541 tctgaaacta aggacatccc atgcttgttt agtctttttc taacatcttt cattgtgctt
      601 tctaccgtcg acctgctcac gtataattcg tcagcaagat tttgaatctt tagagaatca
      661 ttagtattca ataatttagc aaacacataa gttagacggt cctccggaga gtctgcgata
      721 ttgattttat gactggataa taaatttcga agtgtttctt tttttccaat aagcgaaacg
      781 ccttttttag gttgaaccaa catttgtaca tctggcattt tttggattat ttctttcaaa
      841 attaaaatat ctttgctaat tgttttcgta gatacatata attgttctga taattctttg
      901 taagttgtgt gctcattttc aatcaaaaag tatagtattt tcatttgccg ttcgctaaat
      961 tcgatgccca ttatcttcac ctcctgaata cagcttaagt gtacaattat tatctctatt
     1021 ttcatacaga agaaagttct tgattaatgt agaagtttct aatattcttg attatttcaa
     1081 atcaataaaa aagcctgcaa tatttatatc acaggctttt ttctctatta aatatatata
     1141 agataataca tttttctact agttctcact aatcttgtat aattaaaccc tccgcaactt
     1201 gacggagatg gagaagatgc ccccgtctaa tctagcggtc atctctcctc ccattccttc
     1261 caccaatttt tttgacaggt aaagcccgat tccgctagat tccgtatttg tgcggctggt
     1321 attttcggta taaaaacgct ggaagacttt ttcaacagag gtttgcgttc ctggtttgat
     1381 aggattggcc actgttaatc gaatgaatgg atcctctttt gaaaatgtca cctttgcttg
     1441 tccatctgca taacgcaata aattctgtat gatattttgc agcactcgca gcaacatgag
     1501 cggatctgct aatacagttg tatttatcga gtgttcagcg aacgctacct cgatattttt
     1561 ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc acctctttag aaaaagaaag
     1621 cgtatggaga tccatttctt tttgcttcgt ctctattcga gcaagatcat aaaactgatc
     1681 cgtcatactg agtaatcttg caacagattg tttgatgatc gttaaatact tctcccgttt
     1741 ctccgtatcc tctacctgtt ccagtaattg tacataaccg ttaatagcag tcaaaggagt
     1801 tctgagatca tgagaaaggc cgacgatcga tagtctgatg ttttcttcca tttcttgaaa
     1861 tgcttgtcct ttttgttcat tttcatccaa ccatgtattc agtgcatcta ctaggtccat
     1921 tagattcttt tctcgaaatt cgactgaaag acgaccacca tattcttgtc gttcagaaag
     1981 agtggcgatt ttcttagtta atctcttgat ttccctttgc aggaagaaaa taaaacttcc
     2041 cataaagata acaacgagaa gcaacagcca tttaatcaaa tctaaacccc actccccaca
     2101 ccgtatgaat atacacagca tgtgcctttg ctagcttccc tcgaagattg gaaatatgca
     2161 tattcactgt tttctcacta tcaaaatatg gttcttgcca aactgtttca taaagatttg
     2221 ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt cagcagatcg aattctcttc
     2281 ctgtcaaatg gacagccaca ccattcacat ttacatccct cgactctttg tccagtaaga
     2341 tttccttgta atagatgcgt tgcgacagat cttttacttg gacagaagtc tgatgacgaa
     2401 gttggatgcc gattcgtaac aaaagttctt cgatatcaaa tggtttcgtc acatagtcat
     2461 ccgctcctgc tgccaacaag gaaactttat ctctttgatc gctttttgct gaaagaacga
     2521 tgatcggaac attcgatgtc tgtcggattt ctgctaacaa tgcttcacct gaaagccctg
     2581 gaagcatgag atctagaagg atcaaatcga caggatatgt gcttaacaat aattttcctt
     2641 ccgtacctga ataagcatca agaagtttgt atctctgttc aagaacctcc tccagtaagg
     2701 aatgaacagc ttcatcgtct tcgataatca aaatagtttc cagcaggacc acctacttaa
     2761 tgtcagattt ttggaaaata aacattccta gagctgatga taccacgatt gttactagac
     2821 tgaccagaat cattttcggt gcttgcgaat aagttgcttg ttgcgcaaag tccgtataca
     2881 tgagccacgt ttccgtaaac cactccgtta gaaattccaa gcctttgatt tttgcgaaaa
     2941 caccgggaat gaaggccgtt cccatgatat aaagaaaaga aagaagcaaa ctgacactgg
     3001 tacttctagt gatgaatgtc aaagccactg gaacagataa tgtccctaat gtcagcagaa
     3061 gcaatacagg aaatactttt agaacataat ttccatagtt tttgattccc tcaacttcac
     3121 caaatccgaa agcaagtgtg taagcaagca tggctagtcc cgtgatcaaa accacgccaa
     3181 gtgttgtgat caaaagcgta acgagaagtt tcgacacata ataagtcatt cgattccgtc
     3241 ctaaagataa cgtattgcga atcgtaccgt tttgaaattc ttccccaata taaaagcacg
     3301 caaatgctgc gaaagccaga agaatccatg cagccatttt ttctaccatt aaaatccctg
     3361 ttgcaccgtt gatgactgta tccgttgctt ggtttaattc attgccgtat tttgtatgca
     3421 tataccattc gttccaagaa taaaaaagcg gaagtacgac catcagtcct agtgagaccc
     3481 acattttacg gctcttccat acttttattt tttcagcagt taatagatta aacatgtgga
     3541 ttgcctccag ttaattgtaa gaaataatct tccagttttt gcccaactag gtgaatacca
     3601 tccacaggaa cttttgcttc taccaatgct tgattgatgg ctgctacttg gtcaattcct
     3661 tcaaaaagct ggataacacg gctagattgt acaaaataat ccctataacc aagagagtcg
     3721 agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt ttataaattg acgagattct
     3781 tgtgccaatt cttctttcga taattctttg atcaagtgtc cttcatgcaa aataccatag
     3841 tgagtggcga tctgtgacaa ttcatctagc aagtggctag aaagcaagac ggtgatgccc
     3901 cgttctttta ccaaacgttg gatgatttct cgcatttctg ccatccctgc tggatccatc
     3961 ccattcgtcg gttcatccaa aatcaaaaat tctggattcg tgatcagtgc atttgcaata
     4021 gctaaacgct ggcgcatacc taaagagaaa ttctttgctt tctttttccc tgtattttcc
     4081 aatcgcatca ttttcagcag gtcttcgatt tctctatccg acacaccgcc gttcgctgct
     4141 tggatacgta ggttatctct tgcagttaat tctggataaa gagcaggggt ttcaatcgat
     4201 tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc caaataattc gatcgaacct
     4261 tcatcaatcg agatcaaacc cataatagtt ctcataaagg tagacttacc cgctccattt
     4321 tccccaatca atccatagat cattccacgt ttgatttcga ttgatacttt atctaatgct
     4381 ttggcagcac cgtatttttt ggttatacca gttgctttca agaccgtttc atttccaaaa
     4441 tccatactta tccttctttc ttattgatga gtgaagtata gttgacgggt ataaagaaag
     4501 cagccagaaa tgtataaaga aagtgtaaat tttccaattg aatgtcaaaa aaaacaatcg
     4561 gacaatacgt gtctttccta ttatcaggca gtttgttata cgcttgtatc agtttcctga
     4621 ttctttcttt tcgaccaaag tcgtttctct gtattcctct tcactgaaaa tttttgcttc
     4681 tttcagatca agcttgagta attttaccag accggttata aggataacta tagcaagact
     4741 ccctcccaga aaaattagac tgtctcgcag acgtggttgc gttgtcttcc aatcaaagag
     4801 cgcaccaaac agtgctaatc caagtggact ggccaccatg gtaatcgttg ttatcgttga
     4861 cataagtcgt ccttgtatat gcgttggaac ttctcgctga agccagacca tataaaaagc
     4921 aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa taatacatgc cgtttgggat
     4981 ttacaattag ctaccaaata agaggttatg acaaaagact tttcacaatc ccttcttccg
     5041 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatattta tcaaacatga
     5101 gaagcaattt ttgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     5161 agaagcggat aaataatatt gtttgatgat actttatctt tttcccgttt gtttatagat
     5221 cgaatgatat tatagaccaa gaatcctacc tattaacata gctaaaaaca caaaaaaggc
     5281 actttccaaa ttgtggaaag tgccagaaaa gttaggatta aggtaactgc gctcagcatt
     5341 gcttcgccca gtacgattct cgtaactcgt gaactcgaac gataatcgca attaacgttt
     5401 tgagaactgt gaagctttac gggctttctt aagacctggt tttttcttcg tatcattgaa
     5461 taagtttgaa aaaccttgat ttataaaggt tttcccgtaa atatatttta ttatatttga
     5521 ttaaatttga aatgtttgtg ttctaaaatg tgttctaatt taaaggcagt atatttcgta
     5581 tt
//
LOCUS       DAHHPA010000131         5358 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.136, whole genome shotgun sequence.
ACCESSION   DAHHPA010000131 DAHHPA010000000
VERSION     DAHHPA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.136"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..906
                     /locus_tag="NBH55_002589"
     CDS             <1..906
                     /locus_tag="NBH55_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1510518.1"
                     /translation="VKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHF
                     NEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVL
                     SINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFL
                     NLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFA
                     MPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIAS
                     NHELLANLKTSSSFEKLVYILKEGL"
     gene            907..1353
                     /locus_tag="NBH55_002590"
     CDS             907..1353
                     /locus_tag="NBH55_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1510519.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            1369..1683
                     /locus_tag="NBH55_002591"
     CDS             1369..1683
                     /locus_tag="NBH55_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCD1510520.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            1709..2758
                     /locus_tag="NBH55_002592"
     CDS             1709..2758
                     /locus_tag="NBH55_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCD1510521.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            2770..>5358
                     /locus_tag="NBH55_002593"
     CDS             2770..>5358
                     /locus_tag="NBH55_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCD1510522.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLILIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSIL"
BASE COUNT         1909 a          772 c         1081 g         1596 t
ORIGIN      
        1 gttaaaagac tcaaattggg agtaaatatc tataaccctt atacggacaa aatcaaatat
       61 tcttttaaaa ggtcatttat gataagtgta gatttattag aggaaatcga agagagattc
      121 tgtatacatt ttaatgaaga tgaaatcgct tacgtaacac tccatattca atcacttttg
      181 gatcgttaca aacctgacaa aacgaaagtg atattagttt gtagtagcgg atatggaaca
      241 tcaaaattat tagagcaaag aataaataac ggttttgcag atatggttga gatagaagat
      301 gtactaagca tcaatgaact acaagataga aaagtcactg atgaacttgt tataagcacg
      361 attccgattg aaaatacaaa ttttcctgtt attgttgtta gtcctttgat gttaggggca
      421 gatgtcagaa aagtaaaaca gtatctacag ttaaatgaac aaacagaaga tgcttttcta
      481 aatttattag ataaacgatt ttgctttttt acccaccaaa taaaggataa tcaagtgaac
      541 gttttaaata taattaccaa aaaattggta aaagaaaatt acgcgaaaga agggatacta
      601 gagggagcaa tcaaaagaga aaaactatct tcaacagcga tgaatgattt tgctatgccg
      661 catatcgaag tagattttat aaaaaagcca gtaatctgtg tctacgttaa tccagaagga
      721 attgattggg atgggacact tgttcattct gttttctttt ttgcacttaa tgaaaaagta
      781 aaacctgaat tgaataaaat ttatgaaaca ttcaatgaaa tcgcttcgaa tcatgagtta
      841 ttagcaaatt taaaaacgag ttcgtcgttt gaaaaattag tttatatatt gaaagaaggc
      901 ttataaatga tcagtgaaaa aatcaatata aataatatag tgatagattt aaatgcatct
      961 gataaaaaaa gtgtagtcga aaaattatcg aaattgttag agactaatgg gtttgtgtct
     1021 gaccacaaac aatttcaaaa agacgtattt gacagagaaa accatatgac cacaggaatt
     1081 gggaaaaata tcgccattcc tcatggcaaa agcctagcag taattgaatc aacagtggct
     1141 gtagccaagt taaagcggtc tgttgaatgg ggcgcattag atgatcaacc agttgatctc
     1201 gtctttttgt tagcaataaa agacactgat aaaggtacta atcatttacg tattttagca
     1261 gaattatctg gaagattgat ggatgacgag tttgttaaaa gaataaaaga ttcgagaaat
     1321 aaaaaagaat tggtaaaagc tctatcattt taaaaggagg aatttcatat gacaaaaata
     1381 gtagcagtaa cagcatgtat cgcagggatt gcccatactt atatggcaaa agctaattta
     1441 gaaaagtttg cgaagaagaa agggttagat attaaagtag aaacacaggg ggctatgggg
     1501 atagaaaaca aactttcaat ttcggatgtt tccgaggcag atgttgtcat atttgcagta
     1561 gatacaaatg tttcagaacg aggtcgcttt gacggtaaac cgattttaac agtagggacc
     1621 tcagaggtag taaaagatgg tgaagagacg attgatgaag caattgcatt aattaacaaa
     1681 taaaaaatat atcgtggggg agttactaat ggaattcaag aaacaaggaa gagaaattct
     1741 aaatcatttc caaagtgggg tttcctatat gatcccgctt gtcgtagcag cagggttact
     1801 aacttctatt gcagtcattt ttggtggcac aggtgtttgg gaccagacgg atacattctg
     1861 gggagtcctg cgaatgattg gtcagacggg attacagttt atcgtaccga tgatttcagc
     1921 ctatatcgca tattcaattg ctgaccgtcc cggtctagca ccagctttta tcactgggat
     1981 gatgtgtcaa aatcttggta tgggatttat tggaggaatg gttgctggat tagcttgtgg
     2041 ctatttagct aatgcattga aaaaagtaaa agtaccgatg caagttatta gtctaaaatc
     2101 aattatgctg attccttttg ttactacatt agttgttgga atcatattat ggtatgtact
     2161 agctacgcct attcaagcaa tgacaacagg actgactaat tggctagaag gcatgtcagg
     2221 agcaaatgct gctttgatgt cggctatttt aggaggaatg atggcgtttg atatgggagg
     2281 acctatcaat aaaatcgcat atgcttttgg ggttgcttct ttttcaagcg gaagctttgg
     2341 acctagtaca gccatgttat taggaattgc tattccgcca tttggaatgt ttttagcaac
     2401 aattttaaac aaaaaacttt atgatgaatc ggaacgagat aatgcaaaga cagctatgat
     2461 tatgggcctt gtggggatta ctgaagggac gatcccgttt gccgtgaaag atcctttacg
     2521 agtaatacca agcatcgtgg tgggcactgc agtagcatgt ggtctaaatg gattttttgg
     2581 tgttactcaa gaaacaatgc tttcaacctt tatggcaatt ccttttgtta cgaatatacc
     2641 tttatattta gtatcaattg ttattggggg agtggttaca gcgttaatgg tcaatgcact
     2701 aaaatccatc aagtataaaa aagaacaaca agtaaattca gtagaagaag gaaagtagga
     2761 gaatctgata tggtaaatgc acatataatt caacacacgc actgggacag agaatggtat
     2821 tttacaaatg aagatgccat cgtattgtct gatcaggtct ttactgaagt tctagatgaa
     2881 ttggaaagga accctaaagt aaacttttgt ttagatggac aaacttcaat cgtcgatgag
     2941 tatttggaga tcaatccgca aaagcttaag gttatccaac gactgatgaa aacaggacaa
     3001 ctattcgtgg gaccgtggta tgcacaaaca gatgccttat tagtggatgc tgaatcaatt
     3061 ttaagaaact taatgattgg aatcagtgat acgatcctta aatatggaga tccaatgatg
     3121 gtagggtatt tgcctgacac gtttgggttc aatgcgaatt tgcctacact tttgcatcaa
     3181 gtagaaatcg ataatttcat gtgttggcga ggattaaact ttgaaacgat ggttccatct
     3241 ccttatttta tctggaaagg attgggagat aaatttgttt atgccatgaa ttttccattt
     3301 ggttatatga ctggaatgat gaccttagac gcaattaaag atattcctga atttgcaaaa
     3361 gaaaagttgg atgaagcagt tgaaatgctt catatgcaag gaaaaaacaa agatatttta
     3421 gtaccttcgg gtgtagatca aaagtcaatg attttaaatt ttgatcaagt cgtagagaag
     3481 ttgaataaat acagtaaatt ccacaatatt atttcagatt atccttctta tgtagaagtt
     3541 atcagaaaca aaaaagaatt acctgaatat aaaggtgaac ttcgtcagcc ggtttactct
     3601 cgtgttcatc gttctattgg ctcagtaaga acaaaaatga aaatagaaaa ttttaaattg
     3661 gaacaaaaaa ttctaaaacg agttgagccg ctcatactga ttgcagaaag aaatggtatc
     3721 catatcagta aaggcctact tcgtcggtta tggaaaaaga ttttgcaaaa ccaggctcat
     3781 gatagtattg gtggctgtgt ttcagataat gttgcggaag atattttcca tcgaatcaaa
     3841 gaagctaatg agatagctga cggaatcgaa aaccttattg taaaacggat ggctgatgca
     3901 ttaaagctaa aatcgaatga agtccttgtt ttcaacacaa cgactgagaa ttatagtgga
     3961 tcgaaaatcg tgcatgtagt agctgattca aagaaaatac acttcaaagg tagtccctat
     4021 aatatcattg aaaaagaagt ttattttcca gaacggaaaa atgcttatcg gatgattgct
     4081 acaggtttcg aatattttga tgaaccagct tattatgaac ttgacataaa aataaatgta
     4141 aatgtgccag cactaggata cactgttgtt gaatttgaga aaaataatga aaaattagaa
     4201 acagcaatcg agatagacaa aactgagata agtaacaata aatataaact ttcttttaaa
     4261 gatgggcaat tgaatttgat agttggagat agacaatatc ttgatttcgt tcatttgatc
     4321 gattcagcaa atgatggcga tacgtatgat tattcaccat tagaaggaga tacagaacta
     4381 tctctaaaat tagaaacagc taaagtatat aaagattctt tacaagaaac attagttgtt
     4441 tacggaaaag ctcaacttcc taaagattta aaagatcgtt tatctgaaaa acccgaaatg
     4501 gaagaaatat cttatgaaat atcgttcagt ctgggagaaa gtcaaatcgt tgaaggcaca
     4561 ctcaaaattc ataatactgt ttactcgcat cgtatgcgct tagcaattaa tgttttagat
     4621 aaatctgaaa aatcaattgc acaaattcaa aacggattta ttgaaaatac ccctgatgtc
     4681 attcctccga attggtcaga aactatggta gagaaacctg ttaatttaga aatattcgat
     4741 aagagtgtct ctgtggcaga aaatgatcat tattttacgt tttatgcaga tggtttaaaa
     4801 gagtatgaac gtatagaaaa tcaattaatc ataaccttat tttcaacaac aggggaactt
     4861 ggaaaaccta atcttgcttg gcgacctggg cgagcttcag gagatacgac aaatgaaggg
     4921 catgtcatga tggaaacacc gctagctcaa gagataggtg agtataaagt cacttttgga
     4981 tttaatgtag aagaaggaag atttaatgag ttcaaagtgg caaaacaggc agaaaaacgg
     5041 ttagaacaat ctatttctta tcaaaagcag aaactgaatg tatttattca caggttagat
     5101 aacaagattt ggccattaca aaatgaactt gatgttcctc gtaaattttc attgattgaa
     5161 ttacctgaag acttaattgt atcatcgatt tatccttctt attttcatga acaaggattt
     5221 gttcttcgct tagctaatcc aacagaacaa gaaaaaatag tacctgaggc tatcctttcc
     5281 cttgggacag ttgtcaatgc actggagaat aaacaagaat taacaacaat tcctccctat
     5341 gattatttgt caattttg
//
LOCUS       DAHHPA010000132         5331 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.137, whole genome shotgun sequence.
ACCESSION   DAHHPA010000132 DAHHPA010000000
VERSION     DAHHPA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.137"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            11..2338
                     /locus_tag="NBH55_002594"
     CDS             11..2338
                     /locus_tag="NBH55_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1510523.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2464..2793
                     /locus_tag="NBH55_002595"
     CDS             2464..2793
                     /locus_tag="NBH55_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCD1510524.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2833..3837
                     /locus_tag="NBH55_002596"
     CDS             2833..3837
                     /locus_tag="NBH55_002596"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCD1510525.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3834..5263
                     /locus_tag="NBH55_002597"
                     /pseudo
     CDS             3834..5263
                     /locus_tag="NBH55_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         1891 a         1027 c         1057 g         1356 t
ORIGIN      
        1 gagaggaggg atgaaaatga ttaagaaaac tcaaaataaa atccaacgta tatgtcaaaa
       61 acattttgtt tctagactca aagctgtatc taaaagaaaa tacttacatc ccattatgat
      121 ttcgatcatc ttgattttta ctttcttagg gagcttatca agtatttatg taaacgttta
      181 tacagaaacg tcaaattttg ctaaccaaac gacagaggag acaaatagtc caccctattc
      241 tgaaatacag tccatagaaa gcaccaatca gtctagcaac aacgagacaa cctcaaccac
      301 atcctcgact caactgattg atccgacaga aaaagaaatc acagcagcaa aagcagcagg
      361 ggagaaagct tattcagaaa caggtagaag ccagacattt agtactgttg cggccactgc
      421 gaatttcaac ggacatacca taccagatgg atatacagtg atatatgcca aagatggagt
      481 cattgttgcg gttaacggat cgatgagtgc ggataaaact gcgttacaag gcgtaaccaa
      541 agcaatcgct tttattgata accgtccaga tatccataat ctgtttattg gctccacagt
      601 aaatggtact actatcacat tcccaaaaag tatctatatg ggggtggcta ccgctggaaa
      661 tacgatcgat tcagttacac ccaatgcgga taaaagttat tcttttacac cctcaacatg
      721 gggagggctt cctgccggag gaggtatgag tactttttca atcggtatac agttagcaaa
      781 tgatattcca ttcacggggt actcgatcaa aaatgggcag atcgttgatg gtaagggaaa
      841 tgtttcaaat tttccaacct atcaagcagc aacggttcag gtcaatgcga atcggacagg
      901 aactttttct tatacagtca atggtggagc agcaaaaatc ggaacgtacg ggactacatt
      961 atctggaatc catgtgggtg acaaagtggt tatcactcca acagcagtaa cgggctattc
     1021 ttttgcacaa acaaacggtt ctttcacagc cctagaaagt aacgtcgata cggtgaccta
     1081 tacaggcaaa caaacaccaa tcactattaa gtatcaagac tattttggac agatgattgc
     1141 tccttctaaa ataatgaatc ttacttacgg aagtgctgca caagacctga cgactaatac
     1201 gcctactatc aacggatata cctttacagc tgtttcagcg acagaaacaa agaaccaatc
     1261 agcaatgagt gttttagcta gtttagatac aaatggaaat gtaatcgtaa aggacgcaaa
     1321 tggaaaacag atttctgaag tcatcctata ttataagaca aatgcaacag tatcaatcaa
     1381 tgcaaatggc agtaagtact atgatggtct ttctgttgta ccgatcgtta aatatgcctt
     1441 taatgataaa acagaatcaa catccctttt aaacaatgat ctcagtattg atcaaatcac
     1501 atggaatcca actgatttca aagcgatgaa tgaaaaggga gttgaagtaa ctgcgcctac
     1561 tgaaattgga acctatacgt cttggcaatt gacaaaaagc ggactagcta aactagctgc
     1621 cagaacaaac tatttattta ctatcgtgca gacatctgat gtctatacga ttaagcaaat
     1681 cagcggtgcg gtagagctca gtggcagtaa gacatatgat ggaaaagcag gtgtcccgtc
     1741 aatccatgtc aagctagcag agggtgtcac tagttcgctt actccagtac aagtggcact
     1801 tagcagtatg gattacacag cagatgcaca atcagcaaaa aatatggtta atgctggcag
     1861 ctatacaatt aaactaacga atagcggaat agacaaagtc aaagcagcaa atcccaagtt
     1921 gtcatttact gatatagcca acacatcagg aacttatacc atctataaag ctaacgcagt
     1981 gattactgtc gatgacgctg cctttaatta tgatgcccaa agtcactcga tttcggttgg
     2041 aaatctccac gttaccggtg ttgtcttagg cgaatcattg gattataccc taacagttaa
     2101 cagccggaca gatattggat cacaaatagt aacgattagt ttaggcgaag gaagtgtaaa
     2161 caataattat acgattatcg caacagatac cgctaagttg acgatcaatc cgactctctt
     2221 gcttccttcg acaggtggga taggtctact gccctttgtc tttttaggac tgatctttat
     2281 tttttctggc ttcttttatt ttatccatcg taagaaaaaa gcaggtgaac agcgatgaaa
     2341 aaaacaaaaa atgtacgtgt ggatatattt gctgttctct tttttatcct actggtcttt
     2401 tttataggtt ctccgactgt gtcagctaga gaatcaccta ttacatttac tttgcataat
     2461 cgaatgttcg aaagcgataa gatgccaagt atcagtcaaa acgacggaat aaatatcccg
     2521 gataactttc aaggagtaaa agggttgaac ggcgttactt tcgctatata tgatgtctcg
     2581 gatgaatttt acaagctgcg gtcagaagga agtagtgtgg aagacgcaca aagaaaacta
     2641 gcacaaaaaa gtgatagtga aaaaatactg gcagaaaatg tgacaaaaac agtagacgag
     2701 gaagagggca ttgcttcttt tagtgcatcg gacaaagatg agcaaggtcg tgacgctgtt
     2761 taccgatttg cggaaataaa gacttcagat taagtaaaag aaaaatcagc accatttgtc
     2821 gttgtgcttc ctgtgacaga ttcatctaat cataaattga cgaccatcca cttgtatcca
     2881 aagagcgaac aaaaaatacc tgctgcactc acattaacaa aaacagtaga gaataagcag
     2941 acagattttg cagatgggga taaagttcct tatctcatca ccgtaacaat tcctgagaac
     3001 atcaacgaga taggaaccta tacgatcaaa gatacagctg atcctcagtt atggctagaa
     3061 ccagaaacga tcgatttttt gattggtgga gacaagattc atacatttca cacacaaaaa
     3121 acaaaacatg gttttgtctt atcagattct ggaaaagatc ttagttcgtt tgctgggaaa
     3181 aaattaacga tccgttacca aatgacattg aaagaaaaca gtgaaaaaac gacatttgat
     3241 aacgatgtac gtttgacgac tgacaatcaa acagtagaaa cacatcttgc catccaaaca
     3301 ggaggcaaac aatttgtcaa agtggatcgg caaacgaaac aaaaactagc agatgctcaa
     3361 tttctagtca aaaaaaatga ccaatactta gcgcaagaaa acgggataaa tcattgggta
     3421 gccaaagatg attcaagagc aacgattttt acttccagta aagatggaac attttctgtc
     3481 catggattgt cttttggaag ttacgaacta gtggagatta aaccgcctaa aggatatata
     3541 ttgagtcaat caaccattct ctttgaagtt gaaaaaggca gttatgatcc gcttcatcct
     3601 ataccattgg aaatcatcaa cgaaccaaaa gcaaatccga cgaataatgg aaaaacgcca
     3661 gttcagatgc agacaaatgg tccatcaaat gaaaaaaatg gaggaagctt tccaaaaaca
     3721 agcgaagaga tgcttggagg tttctcaatt ttagggttgt tgctagtcct ttcaacaggg
     3781 acagcttggt tttataaaaa acaacaaaaa ggaaacaata ggagggaaat aaaatgaaaa
     3841 acatcaaaac aaaaatatgg acgtttttag gaactgctat catgctgctt cctttcgttc
     3901 tcggcctagg aactgcagag gtttctaggc cgtatcacct acaccagaaa atgttacggt
     3961 taatttgcat aaattgaaat tcacttcagc accagaaaat caaataaaca acggaacgga
     4021 attgacattt ccgaatagcg aaccgttgaa cggcgttgaa ttcaacgtat atgacatcac
     4081 tgctacgtac tatccaagta aagacacagc agttccagca gacgctacac ccttcgcaag
     4141 tgtaacgact tctggtgaag gattagctaa cctgactctt ccaggtaaat ctgatgggaa
     4201 agatgctgta tacgtctttg tagaaacacc aaagcccggt gtcgaaacgt cgccaaatat
     4261 cgtgttaagc ttaccctttt ataacccgaa ttcatcagac cctgacaaac ctttagacac
     4321 gattcaccta tatgctaaaa atgtcatcaa agaaagcaat atcaaaatcc agaaagctgg
     4381 gaacttttca ggggtagata agttagcagg ggcgcaattc cagatcaaaa attcagagaa
     4441 tcaattcgtg accggttttg atgaaaacac tggtgtcgct ctctatgatc ctgctgagtc
     4501 agcgttgaca ttcacaactg atactgaagg atccatcagt gtcaatggat taatagacgg
     4561 caattatact ttagttgaaa caaaagcacc agatggttat caattaacca atccacaaaa
     4621 agaaactact tttatagtaa aaaacggaaa attgcaacaa acaaccggta tcatccatgg
     4681 cgcagatgaa gccaatcttg ggaatccaaa cggtgtagtc aatcgggaaa atacaactac
     4741 ggataacacg attactgtag aaaatgtcca aaactacggt gatttccact tcatcaaaca
     4801 agatgtgaac acgcaagaaa aattagccga tgcagaattt tctattctaa gagcaaaaga
     4861 tccaactgtt aaattaatga aaaaaatagc aaatcttgcc tcaggagaat acccatatgc
     4921 ttggtcagat gaagtcacag gttctgactg ggaagaagtg aaattgattt ctgattcaga
     4981 agggaaattt tcaatcgaag ggctgaaaca aggggcatac tgccttcaag aaacaaaagc
     5041 accagaaggc tacgcattac ctaagaatga agctgcgtac actgaattta cagtagatga
     5101 tgatccaaca acttctgatg aaacaacgat cgataaccaa cctaaaggaa tcttgccttc
     5161 aactggtggt atggggatca tcgtattcat cttggtaggt actgctttag ttggaggagc
     5221 agtgatctac ttcaaaaaac gccatcaaga aacagaagct taaactattg gaaacaaaaa
     5281 tcatacgata aataaacgat agtgattcaa atggggagaa aggttgtgac a
//
LOCUS       DAHHPA010000133         5302 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.138, whole genome shotgun sequence.
ACCESSION   DAHHPA010000133 DAHHPA010000000
VERSION     DAHHPA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.138"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(136..1284)
                     /gene="nagA"
                     /locus_tag="NBH55_002598"
     CDS             complement(136..1284)
                     /gene="nagA"
                     /locus_tag="NBH55_002598"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCD1510526.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1360..2196)
                     /locus_tag="NBH55_002599"
     CDS             complement(1360..2196)
                     /locus_tag="NBH55_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1510527.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2380..3597)
                     /locus_tag="NBH55_002600"
     CDS             complement(2380..3597)
                     /locus_tag="NBH55_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCD1510528.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3746..3925)
                     /locus_tag="NBH55_002601"
     CDS             complement(3746..3925)
                     /locus_tag="NBH55_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510529.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3980..4063
                     /locus_tag="NBH55_002602"
     tRNA            3980..4063
                     /locus_tag="NBH55_002602"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4014..4016,aa:Leu,seq:gag)
     gene            complement(4303..5301)
                     /locus_tag="NBH55_002603"
     CDS             complement(4303..5301)
                     /locus_tag="NBH55_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510530.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1647 a         1077 c          841 g         1737 t
ORIGIN      
        1 tttacattgt tacttattga acaataggaa attaaagaaa cttttgaaac ttatgtaaag
       61 tgatttttac tagaacacaa aaaaacctta aaaccaaact taagaagttt agttttaagg
      121 ttaacttaaa ttatattatg cgtggaaacg ttccacgcca tctagataag ttgcttccaa
      181 tgtcatatca gggttcaata cgataaagtc tgcatcgcgt ccttgagcga tttttccaca
      241 tgtatcatcg attttacagc tgattgctgg aatcagacta gccatcatga ctgcttgttc
      301 tggtgttgct aatccccagt ctacaacatt tttaattgct tctttcaatt tcaatatact
      361 tccagctaag ttgccagatt ctagacgtgc tgttccttct tttactacta ctggaaattc
      421 gcctaaattg taatttccat caggcatccc accggccatc atacagtctg taattaatgc
      481 gacatgatca tgtcctgctt tttccattag gattcccgca gcatttggat gtacatgatg
      541 gccatcacaa atcaattcag aaaagacatg ttctaaagat aataacgcac ccaccatacc
      601 tggttcgcga tgattcaatc cacgcatacc attgaaagca tggacaaata cacttgcgcc
      661 tgcttcaaca gctttttgcg cttcttctag tgtagcatta ctgtgtccta gagaaacaac
      721 tactccttct tcagtcactt gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa
      781 agcaattttt ttgattaaac cacctgaagc ttcttgccat tcatggaaag tatccaaatc
      841 aggatccccg aaataacttg ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc
      901 ttcaaaatag attcctttga ttttagctcc tggtgcttcc tgatacattt tgccgattgt
      961 ctcagctaca tcttttaaac gttctttgct tgacgtcaat gttgttggta aaaatgaagt
     1021 cactccacaa gataacagac cttcagacat cactttgatg ccttctgcat cattatccat
     1081 tacatcatga ttcatatagc cgtgaatatg tgtatctacc aaaccaggag caatccattt
     1141 gccgctttgg tcaatgatgt tgacttctcc ctcaggtagt tctttggtgt attcaccaaa
     1201 aagtccatcg gtaatttcaa gatagcctgc atgtttgaca cctgacgcta aaaagaattt
     1261 gtcagcatag ataaaagttc tcattctatt ctctcccttt gtttttctac ctctaaagta
     1321 ctcttattta gagaagaagt caagaatggt ctacaccatt tacaaatttt ttgaaatttg
     1381 tttcacagct tgtgctaagc cttcatagaa tgctgctctt tctggttctt ctttacgctt
     1441 gtatattaag cttttaagca caaataaatc attgaggtaa tagtagctat tcttctcttg
     1501 agcccaatag attccttgat tcacctgttc taacgcttcg tcgatttcat tttgatggaa
     1561 taagaaattt ccataattat aaaaaatttg cgaaaattca ctttttgatc gttcatcagc
     1621 tgattgatgc agcagttgga tgcttcgact caaatagtag cgtgcttcag ccgtttttcc
     1681 tgccacacca tatatttttc cgatacaact aatcagttgt atttctgtgt cagaaacatg
     1741 gattctttct ttttggctag agtagcttaa tgcttctttc aaataataaa gggactgaga
     1801 gatattttga cgcaacgtga attcgcaaac accgagataa taataatagc gttgaaagtc
     1861 agttgcttga tacagatttt cagaaatatc agaagatttt agtacttgtt ctaacttccg
     1921 ataatcacgt ttttggaaac aactatccaa cagttcaaat aatttatcgt ttgtccgagt
     1981 agtttttcga gagagattca tcacatgatc aatcgttacc cctagccgtt gacaaatctg
     2041 catcatcacc aatacattcg gcagttcctc attattttcg atccgactta atacactttg
     2101 tgaacaaata tcatgcgaaa gcattttttg cgtcattttt tgttcttttc gaatctcttt
     2161 aatgatcgtt ccaaaggact cgattaacgt ttccataagt atcctcccca aaatatgcgt
     2221 attcgcattc tattcgtatt ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa
     2281 ctaaataata tactttagtt atttaagaaa actccactag aaagagagcc tcagtgtgtt
     2341 tgtctgtggc cctcctactg aaaaatttgg tttttaaaat catttttggc gataggttgc
     2401 taggaattct ctcattttct tagctgtttg tttcaaatca tccattttag gaagataaac
     2461 gattcgaaaa tgatctggct ggttccaatt aaatccacca ccatgtacaa gtagaatatg
     2521 atgttcatgt aaaaagtcca aaacgaattt ttcatcatcc aaaatattga atcgctttgt
     2581 atcaattttt gggaatatat aaaaagcagc ttttggtttt acggctgaaa gaccaggaat
     2641 atcattgatt gcattataga tatattctct ttgttcatag atccttccgc ctggcaggag
     2701 cagctcatcg acactttgat aaccgcctag cgcggtttgg ataatttgct gagacaatac
     2761 attggaacac aaacgcatgg aggacaacat attcagccct tctatatagc ctttgacgtg
     2821 tgacttatct ccacttagga ccatccagcc tactcgaaat cctgctacac gatgagattt
     2881 ggatagaccg ttcagcgtaa caacgaacaa atcaggcgct aatgtagcaa tcggaatatg
     2941 ttctagacca tccatcacta agcgatcata gatttcgtcg gaataaataa tcaggttatt
     3001 ttgtcgagct acttcaacga tttcttctaa taattctttt ggataaagtg ccccagtagg
     3061 attattagga ttaatgataa caatagcttt tgtccgactc gttatttttg atttgatatc
     3121 atcaatatca ggataccatt ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc
     3181 agctaatgca atagaagccg tccacaacgg atagtccggc attggaacca gtacttcatc
     3241 tccattgtcc agcaacccct gcatacacat cgtgatcaat tcgctcactc cgttgccggt
     3301 gtagatatca ttgatcgtca cattcggaaa atgtttcagt tggcaatatt gctcaattgc
     3361 ttttctagca gaaaagatcc ccttggaatc tgagtagcct tctgagtcac gggcattcat
     3421 gatcaaatca cgaatgactt cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt
     3481 gttcaatttt aaaatactaa tgccctcttc ttgcatccgc tccgcttctt ctagaacggg
     3541 accgcgaaca tcgtaactga caccttctaa tttgttggat ttttcaaatt ctctcatcac
     3601 ttagccaatc cctcatttca aaaagttaga actttccaaa ttttttgtta tttactattt
     3661 caatatcata ctccatccta gaaaattttg ccacttattt aggaaaaacg taagtattaa
     3721 aaaaacacaa acaagcgttg agaacttaat tgtcatgata gtcattccat agattcgctg
     3781 cccaatccat tgcaattcca caaatgattc ctagcataaa accaagcaaa aaacgtccga
     3841 atgcaagtcc aatcaataca cctaagatca ttcccagtcc tgaaaattct ccgtcatatt
     3901 tctccatctt aataagtcct cccatttttg agcttgacaa ctatgtaaaa cctcgctatc
     3961 attagtgtgt tattattacg cagatgtgtt ggaattggca gacaagcatg attgagggtc
     4021 atgtgagcgt tagctcgtgt gggttcgagt cccatcatct gcactacaac tcgtgaagtt
     4081 aattttgctt cacgagtttt tttgtttgaa cgtttcttta tgccattata tacatatcac
     4141 tgtatccttt acaaaaagaa cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg
     4201 tcggaaacaa gctactcgta gaaacttgca agtgttaaaa caattaatcg aaaaacgagt
     4261 aaaatccctc acctaatact aaagcgaggg attttttgta tattatctgt ttatcaccgt
     4321 tattataaag gtacaaatac caagcacggt taatccaaat tgcataaatt catttttttg
     4381 tacactcatg cttttcatta gctctttaac tgccattagt actaagatgg cgataacaac
     4441 ataaagtata tagtacgtcc gcattccgga agcccatact gtgggagaaa atcccataat
     4501 taaccttgaa caaaatccca ttatcatcaa aataactaaa aaaatagaag ttttcttatt
     4561 gtcaaaactt aaataaattc caacaattaa acatagtaat aatgccaaga ctactaaagt
     4621 agctatccaa gtacctggat aaaaaagtga cagtttcgtt cctaactctg taaagagatt
     4681 attcaggtta ctactactcc aaatcattgc ccttttattt ccattgacgt aagtaaagct
     4741 ttgacccatt gtattaccaa aataaataat caggtttaag aaaaacggta atgcagtcaa
     4801 tatacgcaca taatagtttt gacatttcct atatgttaga aggaaaatta agaaaaatag
     4861 caataaaaat aagatattca tgtctaaaaa aaacggcttt ccaaatgatg aaatacctaa
     4921 atctaactta ttaagaaagt tgaaattttt atattcagga aaccatttat taatttcatg
     4981 tgcagcacgt aatgcattcc ctggagttgt caaagagaaa attaattctg ctaagcttat
     5041 tattgaaaat actgataact tataattata tcgtccattg acaatcaaat ataaactaac
     5101 tatacttgtt agaacaaaaa aacaggcgtt gacttgttct tgattagtta aaaatatcaa
     5161 taaaggaata cttacccaat atatttttct atttatttct tctcctctta agagttgaaa
     5221 aaacggataa aaagttagta aactaaatgc tactggccac tgatagttca aggtggttgc
     5281 tctccaaccg gtttccccca tt
//
LOCUS       DAHHPA010000134         5281 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.140, whole genome shotgun sequence.
ACCESSION   DAHHPA010000134 DAHHPA010000000
VERSION     DAHHPA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.140"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..323
                     /locus_tag="NBH55_002604"
     CDS             <1..323
                     /locus_tag="NBH55_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510531.1"
                     /translation="IFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASAD
                     QLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKL
                     DFVN"
     gene            complement(349..789)
                     /locus_tag="NBH55_002605"
     CDS             complement(349..789)
                     /locus_tag="NBH55_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCD1510532.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            949..1455
                     /locus_tag="NBH55_002606"
     CDS             949..1455
                     /locus_tag="NBH55_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1510533.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1623..3536
                     /locus_tag="NBH55_002607"
     CDS             1623..3536
                     /locus_tag="NBH55_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCD1510534.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3541..4542
                     /locus_tag="NBH55_002608"
     CDS             3541..4542
                     /locus_tag="NBH55_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1510535.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4744..4950)
                     /locus_tag="NBH55_002609"
     CDS             complement(4744..4950)
                     /locus_tag="NBH55_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCD1510536.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1966 a          809 c         1053 g         1453 t
ORIGIN      
        1 atatttttat aaaacacgaa caacctgtca tattctatat tcaatcaaag tatggtaaaa
       61 cacaggaatt atgggcaaag aaaaggatat tatctatgac tgaacgccca ataaaatata
      121 gtgcatccgc tgatcagtta cctgatattg tcattagcga ttatcctatc gacaaaaagg
      181 catatgatca aacgaataca cagatatttt actggaatgg acaacctaca aaaaatgatt
      241 gggcgcgtct gaaagctgaa atcataaaaa tcaggaatga gaaaaataga tccatattgg
      301 aaaaattgga tttcgttaat taagaaaagc agattgttta tgccaaaatt aagacattgc
      361 aaaaagatta ttttctttaa aatcgacaag atgaccagaa agcaagagtt cgttgagctc
      421 ttgctttctt ttagttttag aaaggaacat cctgatttta tcttcgctaa atccagtcaa
      481 aagttttgca gattggtcgt agataatcgg ttttttcaat aaagaagggg atgtacgaat
      541 ctcaatatat agttcactca aactgagttc atctattctg tgaacaagat ttttataact
      601 aatagaatga gtagagagca tttcggtcac gccgttgttt gtcaatttta gtaagtggat
      661 aaattcattt ttggttagtg gttgacgaat taagttctgt tcgataaacg gtatatcatg
      721 gacgataagc caacgttttg cgcgtcttga agacgagcag ccagaacctg tatacaattt
      781 aatcatcata cattcctcca atagctaagt atttatgaaa taataacatg aagaaatagt
      841 cgaaaagtgc catttagagg tgtaaaataa gaaaatgttg tcacgtataa tggaactagc
      901 tagataagaa atactattaa atcaagaaaa aagaaagaag gcacaagtat gtcaaacaca
      961 gccgacgcaa tagtagtgat cgatttacaa aaaggggtat gttttgattc ttcgccgata
     1021 cacgaattag aaacagtgac agcattagtg aataaaagaa tcaaaacata cgaaacgatt
     1081 gggaaaccag ttattttcgt tcaacacgaa gatgctcaat tagtgccaca aacaaaacca
     1141 tgggagctat tgtcggaatt atctattcct gagcacagtt attttgtgag aaaaacccat
     1201 gcgaattcat tttataagac taatttgagt gttttattaa ctgaattgga ctgtcattcg
     1261 atcgaattat gtggggcaca agcagagtat tgtatagata cgactgtgaa gtttgctcat
     1321 ggattaggat accagcttat gatgtgtaga ggagcaacga caacttatga taatcctttt
     1381 atgaaagcaa aggaaacaat cgctttttac gagaaaattt gggataaacg atttctgaca
     1441 tttttggagc aataagttgt ttcttcgttt aaaaaaagca gaatggatac tggtttatcc
     1501 attctgcttt attacgtaaa atgagaaaat aatcctgttc tatttttatg ggataaacca
     1561 tttatttttt gtataaaaaa tgtttgcgtt tacatataat tctcctagaa acataaaaag
     1621 gagtgaatag cttgagaaca aaacaagtat taaagggagc gttggtcatc ttatttgctg
     1681 taagtacaag cgtcccgcaa tggacagtat atggagagag catttcttcc gaatcggaat
     1741 ctcgggaggt agaaaccaca gaaactactg atgctggaaa tgaaacgaaa tcgagtactt
     1801 cacaaacaga aacaaatact gaaacaacgg ttgagacatc tgttactagc acagatcaag
     1861 aaacgaccgc taccacagat aaccaacaaa cagaagaagc aacagctaca aaaatgtcaa
     1921 acgacgaatc ctcttctgct aaagtaagtg acaaagagaa tgggacagaa aatgatgata
     1981 tcggtaaagg tgaaaatttc aatggtaaag aatatttgaa ttacttcggt aaacagatga
     2041 cagaaatact taatggacca acagaagaca tcaagtcaaa attaatagag ttattagtca
     2101 ctgagcttgc tgttaatgat tatggtgtag ataaagagat tatcaaacat ctgattgcta
     2161 cttatctatt gtacgatatc gcttttgcaa aaaacagtgt gttgatgtcg atgcttggag
     2221 gtacagatga tgggacaacg cctacagaaa acacaggctt ctatccaaat acaaaacaac
     2281 ttcatcaaac tgtactaaac aaacaagatg gcaaaatgat ggatgtatat gcatattatg
     2341 tcgatcaagg ttcagataaa acagtcatga tccatggcgg atttcgaggg aattggaata
     2401 acgggatcgt tacagaagaa tacaatgatt tttataaggc cgggtataat ttattatttg
     2461 ttgattccag agcgactggt aatagcggag gcgattatgt gacctatgga caatatgagt
     2521 cagatgacgt cctttactgg ataaatcaag aagttcgaga gcggccatct caaaagattt
     2581 tactttatgg tggttcgatg ggagcagcta cgatgatgtc tgtattagcg aaagatattc
     2641 ctgtcaatgt caaaggaatt attgaaaatt gcggatttgc atcaattgat gaacaactac
     2701 gttttacgta cagccagaca gtagcccccg cactaccgga tgccataaaa aatcagttag
     2761 acatcattgg ggatcaagag catgaagatt tatttatggg ccttttgaaa caatactatt
     2821 ttgatcagga gatgcatttg gatacaaaag ctgctctacc tacgattgga atgtcagatt
     2881 cattgcctaa attgattatc catggaacag cagatgatgt agtacctgtg tctaacgcgc
     2941 aaaaattata tgagttatca ggagggtata aagatctttt attagtagaa ggagcagggc
     3001 atggaaaggc acaggaagtt gaccatgctg cctacacgaa acatgtaacg gactttttga
     3061 aagtagtgtt ccatgatcag ataaacgtaa agtatgtgga tgaaaacaat aaaagtctac
     3121 tcaaagacca ggacgaaatc cagctttacg gagcttatgg agaaaattat atgacagagc
     3181 aaaagacatt cgaaggatac gaattagcaa atgttgaagg accgactgaa gggattttta
     3241 atgagacgat accaactatt attttctatt acaaaaaaat cccagtagct ccaccaaaaa
     3301 agcaagatcc gacagagaat gcagacaata agggaaagaa tgaacctcga gaggatcaat
     3361 caagtgatga tacgcaaccg attcagatga cagtgaaagt acaaaaaaag acagatgcaa
     3421 aaaaaagaat gttgccgaaa actggagaaa agaaacattc gcttctcatt tattcaggtg
     3481 gatttttatc tgttagcacg attatcacat ggatttggcg aagaaagaga gggtaaatga
     3541 atgaaatggt tcaagcgaat agtgattgca gtaccagtta tcatcgtagt tattttagtc
     3601 actgtatttt tgattaatca aatcacacca aagccgatta gcttgcttgt tagaaaacaa
     3661 tttgacacaa gtgaaaaaga acaagtatat gcccgaccag atgatttcca gcaaaaaatc
     3721 gaacaaatcg atataaaaaa agaaatatcc tatccttcaa agtacaaaaa taataatatg
     3781 gatatctaca caccgaaagt ggaaaagaaa aaattaccga ttcttttctg gatgcatgga
     3841 ggcgcatatg taggagggga taaaaatgat tgtcgtgatt atttggaata tctatgttca
     3901 gacacgcagc aaatcatcgt aaatatagat tatgaacgat caccggaagc aaaacatcct
     3961 acaccagtta tccaattgaa cgaagccatt caagcagcta aaaaagagat aaaagatcaa
     4021 gcagattggt cgaatatttc gattggtgga aattcggcag gtgcacaaat tagtggagaa
     4081 tatttgttag cacttcaaaa tgaagaaatt aaaaaagctg atcatcttga tccgactttg
     4141 gagaacaagc agattacgaa gtttatttct ttgtctggtc tgttggatcc ttctgcactt
     4201 actcaagtaa gtgataaaat ctctagcttt ctttatgaaa aatgtggatg ggcttacttt
     4261 gataataagg actttgaaca atcagagaag gtcaaaagtt tagccttggt cagacatgcg
     4321 gatcgttgga cgcaaaacac attcttaacg gatggaaata caaatacgtt tacgaaacaa
     4381 atggaagaag taacttctgt atttgagaaa gcgggggtga aagtcacaaa agtagattat
     4441 gcggagaaaa atgctgtttt gaaccatgaa tatcagtttg atttcagcaa taaacaagcg
     4501 caagaaacat atcaaaagtt agtaatgttt ttcaaggagt aaagtcttat gatgcaaaag
     4561 aggttgtaat ctgtcactta gctccaagtc acaaggagca atttcaataa acaagctttt
     4621 catggtttat gaatattgta acttgtgact gaggagttag agatggcaag gggcaccttt
     4681 tattcgaaat ttgggaatat cacacgatta ttgtcacgat ctcttttacg taaattttat
     4741 aagtcagatt tgttgttttg atcgtttaaa aaaagcaaca aatccgaagc ctgcggaggc
     4801 aagaagggac aaaaagccga gtggtaagca aaagaatgct ggttccacta atattccgtt
     4861 gttattgata taaggctggg aacaaagatg aatagcaaat aaaatcaatg cactagcgaa
     4921 aaaaagcaaa gaaatatgga tagctttcat aagcagcacc tcctttttta taattgtatg
     4981 ataaatagat taagaatcta taaatgtgac aagtttgtta ggatgtgaat aaaatgatca
     5041 tctagcgaac ttgaaacaaa gatggctaat gataagcaat taatgattca gcaatcagat
     5101 aggtacaaag tttaccatca tacgtctaga ccaataatag aaaatatggg aatataatag
     5161 aaaagattta ggataccccc aaaagaattt ccccaaaaat tcgatgtatc ctaaactttt
     5221 ttattttaca taacaatcat atcaactgtg aaataaaatt acaaatgata acctaaaatg
     5281 a
//
LOCUS       DAHHPA010000135         5245 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.141, whole genome shotgun sequence.
ACCESSION   DAHHPA010000135 DAHHPA010000000
VERSION     DAHHPA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.141"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..486)
                     /locus_tag="NBH55_002610"
     CDS             complement(<1..486)
                     /locus_tag="NBH55_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1510537.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KL"
     gene            complement(497..1165)
                     /locus_tag="NBH55_002611"
     CDS             complement(497..1165)
                     /locus_tag="NBH55_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD1510538.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1295..1879)
                     /locus_tag="NBH55_002612"
     CDS             complement(1295..1879)
                     /locus_tag="NBH55_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1510539.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2210..2443)
                     /locus_tag="NBH55_002613"
     CDS             complement(2210..2443)
                     /locus_tag="NBH55_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCD1510540.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2458..3408)
                     /locus_tag="NBH55_002614"
     CDS             complement(2458..3408)
                     /locus_tag="NBH55_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCD1510541.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3405..4247)
                     /locus_tag="NBH55_002615"
     CDS             complement(3405..4247)
                     /locus_tag="NBH55_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD1510542.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYILMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4249..4968)
                     /locus_tag="NBH55_002616"
     CDS             complement(4249..4968)
                     /locus_tag="NBH55_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD1510543.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1794 a          937 c          658 g         1856 t
ORIGIN      
        1 taatttagag tttagcgata aactacatcc aatagaaatt ttctgccgtt caccacttga
       61 tagcgaaaaa atgtttttat ctaatatatt tgaaagatct aataattcag ctaattcatt
      121 gattctttta tccatatgct cttttgtaaa tccaaagttt tccatcgaaa atactaactc
      181 actcgttgta tttgttgtaa agaattgtcc tctaggatct tgacttacgt agccaatatt
      241 ttttgatata ctttcttgtg ttaaggattg tagcgagtta ttttcaaata aaatttcccc
      301 aaccaatttt ccgtcataaa gtcttggaat aagtccattc attaacttta atagtgtgga
      361 tttcccactc ccagaagaac cagtaatcaa tgtaagactg ccttttttta ttgaaaaatt
      421 aaggtcaaca agcgcttgtt ccttaacatc cttatatgaa aaagaaacat tgttaaattc
      481 aaacaattct ttcacctcaa atcaaaatac ttaagaaaaa aatagtaacg gaaaacagga
      541 tgatatagtc taataagctc atgtttgttt tatagtaaga acttcttttc acaggagatt
      601 ctatcccctt tgttattgca ttagcggtca tttcttcagc aatcatagtt gcacgtataa
      661 gaacgggaac taacagaaat tccaaatatt gcagaggctg aaaaaaagac aagcgaatgt
      721 ttctcatttt taaagattct ttaatcattc ttatttcaga acgataagtc ggaaagaatc
      781 tgatcactac tgtaatactt aacattattt ttttcggtaa atgtattttt tctaaagagg
      841 ataataattc attgctagta tagacactaa ccaacaacca tgagatcatt acgaccggca
      901 acattcgaaa tacaatatat aaggatgtcg caaaaaatat agtaaaaaac tctatcttga
      961 tagcatcaat aattttcatt actaaaaaca gaactgaata aatcaagatg aaataaatac
     1021 atgtctttat tttacgaaac atcaatacta aaactgtagc aataatgaaa caccccactt
     1081 gaaatatatt tccctttcct aaaaaaataa tgaccggaaa aaaaatattt aaagtaatca
     1141 aggttaacgg attgattttt gacactcttt cacctccaaa aaataagatc gcactgttat
     1201 cttaagctga taaaaatgca ccgttattca gataacagtg cgatcttatc aaaaagcata
     1261 actgagaaca attctcaatg caagcttttt ttaattaact cagtaattca atccacccag
     1321 gaacagaaaa ttgatacaat cttctggtta atagcatagt cttttctttt gaatagctat
     1381 gcttcaatat atcatagaat gtagcgtaga atgagtaaat gtagaagtgg atttcttctt
     1441 cattaaagac taaattttga ttgatcattc cgatagtctt taacttatct agacaacaga
     1501 ctgtcttttt cgtcacaaat gatgtaattt tttcaacaat atcttgatat ttggttccag
     1561 ctgattggaa taaaatcaaa tcgaatatac gtttattctc aaaaatataa ttaataaatg
     1621 gatctaattc attgaagttc tctctccaac ttttctctat atctatattt tctaagttta
     1681 agaggtattt ggtaaactct tcattataca tattaataag cgcattaata tgcggctgaa
     1741 taatttcttc aaataacgct tccttagaag taaaatgttt ataaaaagca cccgtagtta
     1801 cgcctgcggt caggcacagt tgccttatct tagtttgctg aaatccttct ttttcaaaat
     1861 ggcttagtcc actttctatt aacttttggt gagttataat ataatctctg gtttctatgg
     1921 gtacttcctc ctaataagat gaattaactt tattatctta cacaggactt gaagtttcaa
     1981 tcgttaacgt ttattactga actcattcct tagagctaaa ctaatgagaa agagcagaat
     2041 aactgataca aaaagcaatc ctacaagaat taatagactc atctaaatca ttcacctttt
     2101 ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag ctattaggat aagtcctatc
     2161 ataatcactc caatagccat tttcttatcc tccctaaaaa taaagtcttt tacttaaaac
     2221 cttttttgta atcgtcaata aattttttcc ctttatcagt taattgaaat cctatatcta
     2281 aatagtcctc atatctaagt ttagccagca tctcacttac agatgctgga ctaacactta
     2341 gaacttttgc taaatcatta ttttttaatt tcttttttct ttttgataat tcctgaattg
     2401 ctagtaaata aattagtata gtagtcgttt taatttgtac catacctacc ctccaaatta
     2461 tttcgaaagg ccttcagcaa ttttatccag gttccatttc atcatcgcat agtaactatc
     2521 accatcttgt cctttttctg caattgaatc agtaaaaatc gttgaataga taggaatatt
     2581 ggtatctttt gatactgttt tcattggtct atcatccaca ctgctttcta cgaataagga
     2641 gggaactttt gttgtgcgta atttttcaac taagtgtttt atttgatctg gtgttccttc
     2701 ttcttcagtg ttgatttccc aaatgtaagc agaaggcaca ttatacgctt tcgagaaata
     2761 tttaaagcat ccttcacttg taacaatcat ttttttatca ttcggaattg aagcaaattt
     2821 agatttagct tctttgtcta gagaatccaa tttttcaata tacttatcta gattttcttt
     2881 atagaatttt ttattatcag gatctttttc cgctaactgt ttttcaatat ttttagcgta
     2941 aataataccg ttttctaaat ttaaccaagc atgaggatct tccttccctt tctcactctg
     3001 accctctaag taaataacat ctatgccatc acttgctgca aaatagtctt tgttttcctc
     3061 tttgttcgca tttttaacta attttgtaaa ccaagcattt cctccagttt ccaagttaac
     3121 accgttataa aaaatcaaat ctgcttttga agttttttga acatcttcag gcaaaggttc
     3181 atattcgtgg ggatctttcc caataggtac gatactgtgt aaatcaattt tatcttttgc
     3241 tatattttcg gtaatatctg ctaaaatcga attagtaact actactttta atttttcgtt
     3301 cgaagccact ttatctttgt ctttactatt tattgaacat gccccgaata taaaaagagc
     3361 aatcaaaatc cccagacttc ccagtactgc tttccatttt ctcatcataa cgatctctcc
     3421 ttctttctta aaaatttttg ttttggtgag attagaaaac taattacaaa taagaatgcc
     3481 gctgttaaaa caatacttga tcctgctgct atgttaaaac tataaccaat aaacaatcca
     3541 agcaatgatg aacttgctcc gactgttgca gaaataaata tcatagtttt caagctattt
     3601 gcatataaat atgctgtagc tgctggtgta attaacattg caacaattaa tatagttccc
     3661 acactttgca tagctgttac tgctactaaa gttaatagaa ccatcaatat ataatgataa
     3721 aaactgacat tcatccctat tgcttttgcc attaatggat caaaagaagt gattaataat
     3781 tctttaaaaa atagaactac tataaccaac actaaaaccg aaacgccaat tgtaatccac
     3841 ttatcaatat cttgtacagc taaaatattt ccaaataaaa tatgaaataa atccgttgag
     3901 ctatttgcaa ctcctataag gataactcct agtgctaaaa aagaactgaa agtaatacca
     3961 attgctgtat ctccttttat tacactattg ctttttataa aagtaataat gatagaagct
     4021 aatagaccaa atacaatcgc accaataaaa aaattaatac ctaaaatata agaaattgct
     4081 actcctggca aaacagcatg agaaatagca tcacccataa gagacattcc tcttaaaata
     4141 ataaaacaac ctactgttcc agaaacaatc cctataacta cagatgtaat tagagcattc
     4201 tgtaaaaagt gaaagtcata cagaccatta ataaaatttt gaagcatatt attccccctc
     4261 tccaataaat atagtatctc cataggcttt ttttaaattt tcttcattaa atacagattc
     4321 tacacttccg tgagcaatta gatttcgatt caaaagaata atattatcaa aatacttctt
     4381 taccttgctt aaatcatgat gcacaattag cactgttttt ccctgatgtt tcaatttttt
     4441 taacgtatcc ataattattc gttcactaac taagtctatc cctacaaatg gttcatctaa
     4501 aaatatgaaa tccgcatttt gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc
     4561 tccagataat tctcctattt gatgattaga atattttccc atatcgacct tttccaaagc
     4621 ctttgaaact ctttgccatt ctgcattttt aattctttga aaaactttca ttccggcata
     4681 tgttcccata gatacacatt cttttacttt tattggaaaa gtaaaatcaa tatcagcttt
     4741 ttgctctaca tatgcaatct tcaccagttt tttattgata tcttcattat taatggagac
     4801 ctctccctga tgagggataa tatttaacat tgctttaagc aatgtggatt ttccagcacc
     4861 atttggacca ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa
     4921 tgctaaaaaa tcgttataac aaacattaag ctttttcact tctaacataa ctttctcctt
     4981 ctaaatgata atgattatca cgatcatatg gtatacaaaa attatatttt agtcaaccct
     5041 aaattttata gtttagattt ttttctgttg ctgaagatta gaatgacaaa actatttgaa
     5101 actacgcgta tagtaagtag ctttcaaaat atataaaaaa ggagcgttct caaatgagaa
     5161 catgctccta aaactacttt attggcgtat ggaaaaatta tacttgaagt ggatagcggg
     5221 ttgaataagt tgataagcat caaaa
//
LOCUS       DAHHPA010000136         5196 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.142, whole genome shotgun sequence.
ACCESSION   DAHHPA010000136 DAHHPA010000000
VERSION     DAHHPA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.142"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..350)
                     /locus_tag="NBH55_002617"
     CDS             complement(<1..350)
                     /locus_tag="NBH55_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCD1510544.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEI"
     gene            complement(347..589)
                     /locus_tag="NBH55_002618"
     CDS             complement(347..589)
                     /locus_tag="NBH55_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HCD1510545.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(1135..1665)
                     /locus_tag="NBH55_002619"
     CDS             complement(1135..1665)
                     /locus_tag="NBH55_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD1510546.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1847..2503)
                     /locus_tag="NBH55_002620"
     CDS             complement(1847..2503)
                     /locus_tag="NBH55_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCD1510547.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(2630..3103)
                     /gene="dfrF"
                     /locus_tag="NBH55_002621"
     CDS             complement(2630..3103)
                     /gene="dfrF"
                     /locus_tag="NBH55_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HCD1510548.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(3522..3740)
                     /locus_tag="NBH55_002622"
                     /pseudo
     CDS             complement(3522..3740)
                     /locus_tag="NBH55_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3771..4985)
                     /locus_tag="NBH55_002623"
     CDS             complement(3771..4985)
                     /locus_tag="NBH55_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCD1510549.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1600 a         1093 c          734 g         1769 t
ORIGIN      
        1 ataatttctt cataaatctt gccataagtt tcatatattt tatccatagc accaatatgc
       61 tccacaacca aatatttata agacggtatt tctttataca gaaagccctc tggggtaatg
      121 gttctttggg gaatagaaca gaaataataa agttttccat ttcttctttc cataaaagca
      181 tattttaccc aatttcctga ttgtgaaaga tacgcttttt tcaaactact gttaaacttt
      241 ctccaaaact gtgtactaat ctgaatattc tttttttgat aattagtcag ttctacttcc
      301 tgcccaataa ctgcaaatgc gtttaattcc tttatagaaa aatttatcat tttaccttgt
      361 gagcagtgat aacggtataa ctgtacgcat ttaccgttat aatacaatta tcaatcgtta
      421 tataccagtt ttttcctttt cttgtgattt ccgcattaga aaggctaatt ttttctttgc
      481 accattcaat tacattttct acattcaaag ataagttctt tttaattcta ataacgccaa
      541 gttcagttgt gtgaagtctt cctaagtttt ccagtaattc attttccata ttttgatacc
      601 tcatttttca gcaaatttat ttgtatgttt tataccccaa ttataacatt ctcatatcag
      661 cactacaata aaaataatta agcaccaata atcttattga acatctccaa ccgtaaatct
      721 ttcacaccat cagtgataaa cactgagctg acagcaatta cgacaactac cataattgcc
      781 tgcacttaaa tttctgcaaa agaaaacgac aatcaatttc ttattttttg aaatcaactg
      841 tctttctcat atttactatt caattttcat agtgatatag gtacaattta tatcactact
      901 gtaaaaatgg ggttttgcct tataaataca gcgttttccg cttgttagtg tatagtgttt
      961 cttgtttttt gtgcccccct tgttagataa cgggggcttt gaacggctcg caagctcgcc
     1021 gaaccgccga agcgaaccgc ccccttgcaa gcatggcgga aaaatcttaa ggattttccc
     1081 gccaacgtgg tcggatcgct ccgtcggttt aacagctttt ctgtttctct ctagttatct
     1141 acgcatataa atcaaatcgg ttattccgtt ataagtctgt gttttacaaa gccttaactt
     1201 tatttccctg tctatgttgc ccaaaagacg aaccccattt cctaacagag taggaataac
     1261 agaaatgtaa tatttatcaa tcatattttt gcttaccaac tgctgaacaa gatttgctcc
     1321 gccacaaatc caaatatcct ttcctgcttc ttgttttagc cgttccaata ataaaacagg
     1381 attttcactt gtaaaatgaa tttgttggga agaattgcac tctttatgtg ttaatacata
     1441 agttgtaaat ttattatata cccattcttg cggagaaagt tcagtaacaa cctgatgaga
     1501 agtattccac cccattaaga cagtatctat atctttggta aattctgtat aagtgtcgat
     1561 gttttcattg tcatttccat gaccatttag ccaagctact ccaccattac cgtccgcaat
     1621 atatccgtca aggctcatgg caataaataa aactactttt ctcatttttt ctcttttata
     1681 aatacaaaca ggaatttatt tctctttttt actcataagg gaacaactga ttgctacaaa
     1741 taaacccatt aaaatccatg gaaatgctgc tcttataaaa tcactaacca tactttattc
     1801 ctccttaaat tccaatttcc ttagttctac aaactgaaat ttttgcctat gaatgtatct
     1861 tgttctttat acgcaaacag gcttctgtgt gcataatcca atgtcttgaa aacggcattt
     1921 gaattagtcc acaaatatct ttattacacc aataatcaat cagccaaatt gctttttcat
     1981 tgtcgtttac tacattcaac gattctaagt atccttttct ttctttcggt atttttcttt
     2041 tcaattcatc ataagataac cgttcaagca ttttctcagt gctttcccat acttcgaaaa
     2101 tatataaata taattcttcc agattaagct gttttgaaaa gtctgcaatc tgctgtttca
     2161 tgagttcatt tcctgttgta atgataggtg aattgatacg ctcttgataa ttgcctgcaa
     2221 aaaatacttg ttcatcttca tttatcactg tatgcacaac tatatcttca atgcggaaaa
     2281 tatgccaaat agaataagca atcgtcttac tatgataacc atcagcgttt atgaagggga
     2341 ttgagttaaa atcttctctg cataattctt ctttgaaaga tactaaggtt tgtattaact
     2401 gacttcgtaa agtaagtaaa gtatcaatac ccctcttata agtatctttc ttttttattt
     2461 gtgcctgcat tgttttatta agttcagacc attccttatt catattcaat cctctctcaa
     2521 attctgattt tatcgcccca tacaaactgg aatttgtctt acaacaattt atatatgcat
     2581 aaaaagcaat atatttatga aaacacccta aatccaaaat ttactcaatt cattttttcc
     2641 ttacataaaa tgttctcgta tatttcactt cctcaccaag tgtttcccct atcaaaactt
     2701 caaaatcatt gatatcaaat tctggaaaga atgtatctcc atcttcaata tttaaatcta
     2761 cttctgtgat atacatttta tctactattt gcaaagcttc cttaaatagt ccatatccac
     2821 cagatatgta tacatctcgt cctttagcca ataataatgc atcttctaat gatttaactg
     2881 aaactaaatt atctccttga tactctgttg tggtggaaac aacaatattc attctattag
     2941 gcaacggatg accgatttct tcataagact ttcgccccat aataaccaca ttacccgttg
     3001 ttaactctct aaattgcttt tgttctccct ttattttcca tggtatatta ccattcttgc
     3061 ctataacatt attctttgac ctcgcaacaa tcaaacctat catctatttc atcctccata
     3121 atatgatgtc gtacctgtga ttccatcaag tgcaccacat cttaacaaac tcaaaattat
     3181 ctcgaactaa ccagctcgac aaattccttt tttgtcttca tctgcattat atcatattgt
     3241 tataagcatt gccatttctt ttctgctttt gaaaagttac ttatcagcct atgttttgta
     3301 aagggtgata ttcagcattt ctcacgcaac aagcccttga caaaccatag actaataagt
     3361 tttaaggtaa tcaagcagaa atgaaatagt ctgctgtttc acggcaacaa aaaagagata
     3421 ccaacacact tatgtagtat ctcttagtct agtagcaata ttcttttgtt tgcaagcagg
     3481 taaacatggg ctgtttctta atatcttttt gtttgggaaa cacctttttc ccatcctttc
     3541 accgttgcca tctctgctgc gcacaatgcc acagggataa accatagcaa accgcctaat
     3601 aacaagaaaa atactaagct gaagcctgag gtcgcgaatg tgggatattc atataatgaa
     3661 acgaccattg agatggtcat agccaaaaag ccaaagaacg ttaatttttt tgcaggaatt
     3721 ttcgttttgc tttgttgcat gaatcttcct cttttctatt tgaatttgtt tcaattgagt
     3781 ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt cttcttttgt agtagtgatc
     3841 gttgtaaagt gcatcgccga ttttaaaatc actgttgtca gcacataggc atacacggca
     3901 gtaatgatga caccaatcac ttgaatccct aagaagtacc agctgcttgg attcgttagt
     3961 aaatggctgt tagatgcaaa gatccctatc agaatcgtac ctgtgaatcc gcccatgcca
     4021 tgaacacccc acacatcaag tgcatcatcc cattgtttct ttttacgaaa atcaacagca
     4081 gcatgacaga cgatacccgc aatgatccca atcagcatgg cactttttgc ttctacataa
     4141 ccggcacaag gcgtaatggc tgccaatcca gcaacggatc ctgttagtac gtcgacaaaa
     4201 tcaaagccat ttccttttac ttttgcaatg atcagccaag taatcatcgc aaatgcaaga
     4261 gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat ctgctcgaag tgcaccaccg
     4321 gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac cgatagcagc tgccataaga
     4381 ctgctatgct gacttctttc tcctaaagga atctttcttt gaccaagtgt gaggatacat
     4441 gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc caccagcgaa atctctaaat
     4501 cccatctgat ccaaaaatcc gccaccccat atccagtggc aaacagggaa ataaatcagc
     4561 agattccaaa aaataacaag tataatatag ccttttaaat tcaatctccc tgcaaaagct
     4621 cctgtcatca aaggaacggt aatcacgata aacatcagtt ggtaaagaaa gaacattaaa
     4681 aatggaatcg ttgcaccatg aaacatgttg gggaagcttg taacatgtct catcaaaaag
     4741 aaatctgccg ggtttccaat aacaccgccg atatcccgtc caaaggccaa tccgaatcca
     4801 ccaaataccc acataatcgt tgttatccca acggagagaa aagattgaga catgatcgtt
     4861 agtatattat tcttccgtgc taatccgccg taaaaaaaag ctagaccagg agtcatcagg
     4921 caaaccattg ctgtacataa aatcatgaat gctgtatctc ctgtattgat aggtaatgta
     4981 ttcatttttt tcgcctcctt tctacgatta cgagtataaa agaaggtgta gttgtttttt
     5041 tcttctgttt ttttgaatta atacttgcat ttataaaaga cagacaatta ataaaagctt
     5101 gatgtgttgt gctctttttc gtttttcaca caaataaaga caaaaaataa acggtcttct
     5161 ttttatagaa aaccgtttat tttcgtcttt ttttat
//
LOCUS       DAHHPA010000137         5192 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.143, whole genome shotgun sequence.
ACCESSION   DAHHPA010000137 DAHHPA010000000
VERSION     DAHHPA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.143"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            13..1020
                     /locus_tag="NBH55_002624"
     CDS             13..1020
                     /locus_tag="NBH55_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD1510550.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1032..2036
                     /gene="neuB"
                     /locus_tag="NBH55_002625"
     CDS             1032..2036
                     /gene="neuB"
                     /locus_tag="NBH55_002625"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCD1510551.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2033..3181
                     /gene="neuC"
                     /locus_tag="NBH55_002626"
     CDS             2033..3181
                     /gene="neuC"
                     /locus_tag="NBH55_002626"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCD1510552.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3154..3831
                     /locus_tag="NBH55_002627"
     CDS             3154..3831
                     /locus_tag="NBH55_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1510553.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3821..4963
                     /locus_tag="NBH55_002628"
     CDS             3821..4963
                     /locus_tag="NBH55_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1510554.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            4976..>5192
                     /locus_tag="NBH55_002629"
     CDS             4976..>5192
                     /locus_tag="NBH55_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1510555.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPI"
BASE COUNT         1924 a          673 c         1025 g         1570 t
ORIGIN      
        1 gaggagatat aagtgtttaa aggaaaaaca atattagtaa ctggcggaac tggttccatt
       61 ggaagtgaaa ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag aatttatagt
      121 agaggtgaag aaaaacaatt ttatatgcaa caagagctct caaatcacac taatttgaga
      181 tttttaattg gtgatatcag agactctcaa aggattgatt atgcatgtcg tgatgtggat
      241 attatttttc atgctgccgc aatgaaacac gttccagcaa gtgaatataa tcctatggaa
      301 gctgtaaaaa caaatgtgat gggaactcaa aatgtaatag atgcagcgat caaaaataat
      361 gtaaaacatt ttgtaggtat cagtacagat aaagtagtga gtcctactaa caccatgggt
      421 gcgacaaagt tattgagtga aaaattaata atagctgcca atcagtataa aggaaaaaca
      481 gaaacaatat tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg atctgttatt
      541 cctcttttta gtaaacaaat aaaaaacaat aaagatatta ctttgacaga tgttgatatg
      601 acacgattta tgatgtcaat accggatgct gcaagattag ttctgtctgc tgctatgaat
      661 tcaaaatttg gagaaacata tgtcttaaaa atgccggcaa tcaatatttt aactttagct
      721 caatcattaa taaaaatata taatgataca catgatgaaa aatatagagg tgaaataaaa
      781 gttgtaggta tcaggccagg agaaaaatta tatgaagagc taatgactac agacgaaatt
      841 gaacgctcat atgaaaatga taaaatgtat atcattccac cacactatca tgctactgac
      901 gaactgtaca aagattatga aaaaattaaa atcactgcat actcttcaga tgcaacagaa
      961 ttaatgagtg gacaggaatt tattagaatc gcaaaagaga ataatgtcgt gacttggtaa
     1021 ggagttttat gatgaaatca gaagtatatg taattgcaga aattggtgta aaccataatg
     1081 gaagtctaga tttagcttta gaaagtattg ataaagctaa agaatgtgga gctaacgcgg
     1141 ttaaatttca aacatttaaa acgaataagc tagtttccaa aaaggctcca atggcaaaat
     1201 atcagaagga taatcttaaa gaggacagtt ctcaatttga aatgctaaaa aaattagaat
     1261 tatctcaatc tgattttatt agaataaaag attattgtat acaaaataaa attgattttt
     1321 tgtctactcc ttttgatgaa gaaagtgcag actttctaaa acaaattggt gtacaaggat
     1381 ttaaaattgg ctcaggagat ttaacaaatt taccttttct tagaaaaata gatatgtatg
     1441 ggttgccagt tttattatct acaggtatgg ctgatattca tgaggtcagt gaggctgtag
     1501 attgtttcat agatagtcca gttactattt tacattgtac ttcaaattat ccggctagtt
     1561 ctgaagatat taatttattg gcgattaaca cattaaaaga aatgttcggg atacccgttg
     1621 ggtactccga tcattcattg ggatatgatg ttggtatttg tgctgttgcg atgggtgcta
     1681 aggtaattga gaaacatttt accttggata aggaactacc tggtccagat cataaagctt
     1741 ctttagatcc tcaggaattt gcagattttg taaagcatat tagaaacact gaaatatttt
     1801 taggtgatgg tgttaagcga gcaatgcctt ctgaacttag tacaaaagaa gtatcaagaa
     1861 aaagcctagt ggtcactgag aatttaaatg tgggagatat tcttacagag aaatctctta
     1921 caataaaacg tcctggaaat ggaatagctc ctaagttttt atatcaatat attggcaaaa
     1981 aagttaaaag accaataagt gcagatagtg ttctgcttga ggaagatgtt gaatgaaaaa
     2041 aatacttata gtaagtggaa cacgagctga ttatggaata tataaacctg ttgctgagaa
     2101 tttactacaa gataattggg atgtatcatt tgttgtttct ggaatgcatt tatcccataa
     2161 atatggattt actaaagaat tgatcttagc tgacggattt agaattagtg gagaagttca
     2221 aagtttgttt ttagagaaca ataagggaaa catggctcgg tctgtggcat tagagataaa
     2281 tggtttcaca aatatatttg aacaaactag ccctgatttt gtattattac taggggatag
     2341 aggagaaatg ttggcagctg cgatagcttg tttgtatctt ggaatcaaaa cagcacatat
     2401 tcacggaggc gaagtgtctg gtactgtaga tgaatcaata agacatgcca taacgaaaat
     2461 agcatctgtt cattttgctg ctacggaaaa aagcaaagag cgtttaataa aaatggggga
     2521 agattcttgg agagtttttc ccgtcggagc tcctagaata gatactattt taaataatag
     2581 attacctagt tttagagata taaaagaaaa atatcatcta aaatttgaaa agaaaaaata
     2641 cgttttgcaa gtatttcatc cagttgtaac cgaactagaa aatatagaaa aacaaatttt
     2701 agctttgatc aattcaatgg aacaacaatc tcaaccaatt atttgcattt tacctaatag
     2761 tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa tatttaaaaa cggatattat
     2821 ttatattacg aatttagagc cggaggaata cctgactata ttaaaagagt gcaaatttat
     2881 gatcggaaat tcttcttctg ggataattga agctgcatca ttcaaaatac cagtaataaa
     2941 tttaggaacc agacaaaatg gacgagaaca atctaaaaat attataaatg caaatctaaa
     3001 tgaaaatgaa attgaaactg ctttgaataa aatacaagat cctcagttta taaaaagctt
     3061 gaaaaatgtt aagaacatct acggtgacgg caacagttct agaagaataa caaaaacatt
     3121 gaatcaaata ttgaatgatg gggaagatat taagtggatt caaaaacaaa tagcttatta
     3181 aaatatacta gtgatccata cattattatt ggggctggcg gacacgcaaa agtcattatt
     3241 gaaaccttaa gattagaaaa taagttaata tatggatttt ttgatgataa gcaaaataaa
     3301 atgcaagatc tgaaatatct tgggaaaata aacgaattga atagctgggc ggatagcaaa
     3361 aaaaaaatct ataaatttat tgtagctatt ggtgactgtg tagtaagaaa aaagattata
     3421 aaaaaattga atatgccaag tgagcaatat ggaatagctg ttcatccaag tgctatagtg
     3481 gcaaaagaaa tttcaattgg ttatggaact gttatttttg cgaatgctgt aattaaccca
     3541 gatgctgtaa ttggagagca tgctataata aacacaggta gcattgttga gcatgattgt
     3601 agaattaata actatgttca tttatctcca ggtgtatgct tagcgggtgg agtacatgta
     3661 ggtgtaggaa ctcaaatagg aattggttct caatgtatcc agatgaagaa aattggatct
     3721 tggtgtatga taggcgcagg gagcactatt gttaaggata ttccctcaca ttccttagct
     3781 tatggcaatc cagctaaaat taagaaagaa ggaattaatt ttgaattcta gaattttatt
     3841 atcaagtcct catatgggag gaacagaaga aaaatatgtt gctaaggctt ttaaagataa
     3901 ttggatagcg ccattaggtg aaaatgtgga taaatttgaa gaaagtatta caaaatatac
     3961 tggttctaag ggcggagctt ctgcgttaag ttcaggtaca gctgccatac atcttgcctt
     4021 aagactgctt aatgttggaa aaaacgatat tgtctttgct ccttcattta cttttatagc
     4081 tacagtaaat cctattttat atcaaggggc tgtaccagtg ttcattgact cagaacctaa
     4141 tacatggaat atgagccctg ttgcactaga gagcgcatta gaagaagctg aaagaaacaa
     4201 ccaattacct aaggcaatca tcgtagtgaa tttatatggt caaatggctg attatgaacg
     4261 aattttagct attgcaaacc aatttaatgt tccagtgata gaagatgcag cggaatcgtt
     4321 aggttctact tataagggaa tttcgtcagg cttatttggg acaattggga tctattcatt
     4381 taacggtaat aagatcatca caacctcagg tggaggagct ctagtgtcta gtgataaaaa
     4441 atatgttgag cattcaagat ttttagcaac acaagcaaga gatcaagcac cgtattatca
     4501 acatagcgaa ataggttata attaccgtct aagcaatatt tctgccggta tcggacgtgg
     4561 acaaatggaa gtattgaatg atagagtagc taaacgaaga gaaattttca atcgatataa
     4621 ggaagctttt tctggaattg atagaattga aatgatgcca gaagtggatg gcatggtgcc
     4681 aaatcgttgg ttgtctactt taacgatcaa tgattataat tctattgatc aagtagttaa
     4741 cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga gctttgtgga aaccactaca
     4801 tttgcaacca gtatttcaag gggtaaaaat gtattaccat gataataatc ttagtgtgtc
     4861 agaaaagtta tttaatagcg gcttatgctt accttctgga agtaatatga cagatagtga
     4921 acaagaattt gttattgatg agtttaaaaa aatattcaag taggtgtgat attttatgaa
     4981 cataggaatt attggtttag gttccatggg gaaaagaaga cttagactca ttcaagaaca
     5041 ctttaatgat attttactat gtggtgttga tactaatgag gcgagacgaa aggaagttga
     5101 aaaggaattt ggaataccaa cttataatag tctaacagag gcaaaaaatt tgtttgaaat
     5161 aaattcagtt tttgtttgta catcccctat aa
//
LOCUS       DAHHPA010000138         5124 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.144, whole genome shotgun sequence.
ACCESSION   DAHHPA010000138 DAHHPA010000000
VERSION     DAHHPA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.144"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(196..885)
                     /locus_tag="NBH55_002630"
     CDS             complement(196..885)
                     /locus_tag="NBH55_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCD1510556.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(930..1694)
                     /locus_tag="NBH55_002631"
     CDS             complement(930..1694)
                     /locus_tag="NBH55_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCD1510557.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(1722..2420)
                     /locus_tag="NBH55_002632"
     CDS             complement(1722..2420)
                     /locus_tag="NBH55_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCD1510558.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2432..3211)
                     /locus_tag="NBH55_002633"
     CDS             complement(2432..3211)
                     /locus_tag="NBH55_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCD1510559.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(3227..4171)
                     /locus_tag="NBH55_002634"
     CDS             complement(3227..4171)
                     /locus_tag="NBH55_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD1510560.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4217..4996)
                     /locus_tag="NBH55_002635"
     CDS             complement(4217..4996)
                     /locus_tag="NBH55_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510561.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1350 a         1079 c          879 g         1816 t
ORIGIN      
        1 agagtggtat catcccaaaa tttcgaatat tttcttttat ataagaataa tgatactgaa
       61 ttgccccatt cacctttctt tttttagatt ttctacaagt gattcaaacc ctccataatt
      121 acagttatta cttttgaaac gataatatga acatgtgtaa tcttttttat taacaatcca
      181 tcattctatc cctaactaat atgctccatt gggcttgaca ataacataaa acgtttttaa
      241 aatcaacctc aagtcgtaca ttaaacttcg atgactaata tactctaaat ccaactcaac
      301 cattttatgg aaactaacat tatttctatc actaacttgc cacaaacctg aacaaccagg
      361 ttttacatac aagcgttgaa gatcatatct cgtgtactca gctacttccc tctccaaagg
      421 tggccgtgga ccaattagac tcatatcgcc ttttaaaaca ttccatagct gtggcaattc
      481 atcaatgctt ttggcacgga taattttacc tattttagtg acacggggat cttctttcat
      541 cttgaacatt gcaccttcta tttcattttg atcaagtaat tcttccaatt tttcttcagc
      601 atctaaacac atcgaacgga atttatacat tttgaatgtt tttccatttt taccaacacg
      661 tgtatggcaa aaaaatatcg gacttcctgg ctcttctttt ctgattcgat aagctacata
      721 gaacatcaaa ggagaagcta aaatcaaacc aataatgctt cctacaatat caatcattct
      781 tttaaaaaat aaatagatat atcgtttttc aactttttct ttatccaacc aaacttgatt
      841 aggatgtact tcactaatac gagataataa atcttcactt tccatacttt ccctctctcc
      901 tcatttcctt tttatctacg aataaagaac taaaataaac caaacttctt cttttttacc
      961 tcttcataga ctggataact gatttcatcc ccgttgatca gatcctttgc gacttgtttc
     1021 atctgtacta ctttttcctt accaaagtcc ttagcaatct gctcatagca ctcttttaag
     1081 taaaaggtac gcttagagac cccatgggca tcagaagcag ccatctgcac gagattatgt
     1141 ttgaccattg ttttggctgt tttctgaatg tctttgccaa atacaccaac ataacttggc
     1201 gcagtaattt gggccaaaca tcccatatct aggaacggaa gcaaatgatt tgggtcttct
     1261 cgaaaatgcg cgttgcgttc tggatggacg atcactggtg tctttcctag ttctaacaac
     1321 tcaaaaaaaa gccattctgc atataacggc acttccagtg tcggaaattc gatcagaaga
     1381 tatgtatcat ctaaatctgt aaataaaatt tcatctcggt ggatgtcttc gatgagcgtt
     1441 ccggtaatac gtacttcttg tccttccaat aacgtcaaag gcaactgtct tttctctaat
     1501 tcggcttgta acgaagcgac tagagaaatg acttgtgatt tttcattgct gtattttcca
     1561 ttattgtggt gtggtgtgca taaaatatgc gtgatgcctt gctgaatcgc tttttcagcc
     1621 attgcaatac ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag gatatggcaa
     1681 tgtagatcga tcatttctct tgtctcctct cttcttattc cttaatttcc gtagtaataa
     1741 ccagcatctt tgctatgttc tgctccgttg tatacaacac ctaaaactcg tgcttgtacc
     1801 atctctagca gttcttttgc tttattcaat gattctttac gtgatgtatt ttcacgtacg
     1861 accaataaag tgccatccgt ttttgaagac atgatctgtg catctgttac ggctactact
     1921 ggtggcatat cgaatatcac cacgtcaaat aactgtcttg cttcgtctag gatctgatcc
     1981 attcttggcg aacttaacaa ttctgatggg ttgggtggct taggtccact aggcaaaatc
     2041 gacaagttct cgactggcgt tcgttggatt acatctgcta cacttcctga tgaactaagt
     2101 gctgtactta atcccctggc attgttcagc tggaatgtct tatagacgac gggtttacgt
     2161 aaatctgcat cgactagtag aactcgttga ccggatttag caaaaacgac tgcaatgttt
     2221 gctgcagtgg tggattttcc ttctccagga cctgatgaag tcacgacaat cgttttgatt
     2281 tgctgaccag ctgcggaggc aaactggata ttcgtccgga tcgtacgata ttgttcggcg
     2341 attggtgaag atgggtttgt taatgtgatc aaactgactg catgagtttg tgctgctttt
     2401 tgtttctgtg ttcgtgccat ttttcttcct cctagactct tgagcggctt ctgcgtgatg
     2461 gggctgtttt atcgtttgta gcttttggtc gtgcaggagc tgttcctttt ttacggatac
     2521 cagcgttcaa ttctttcgct gacatttctg gaactgttcc taagattggg aatcctaatg
     2581 catccgtcac atatttttca tctttcactg tccgatcaag taattcgaat aagaatgcca
     2641 gacctacgcc taccatcatt cctaatacta agccaatcac tagattcaat ttattatttg
     2701 gcgaaacagg tgtcgtattg gcaactgcac ctgaaataat cgagatttta tcgacattca
     2761 tcacgtcttt tgcgttctct tggaaaactt gtgaagttgt gttagcgatc tgttgagcag
     2821 tcactgcatt cgtgcttatt gcctggatcg agaacatttg tgaattttgt gactgattga
     2881 cggagatcgc atctttgatt tgtccagcag tcattttcac attgtcttct gtttctaaac
     2941 gatctttgac ttcatttagt actaaatcac tgacgatcat atctttatac gtattgatca
     3001 tttgcaggtt cgtattgaca tcattgacat ttgctgtgtc tgattgtggc agtgtcacga
     3061 tcaattgtgc ttgtgaactg tattttggtg tcaagatgaa aaatgttacg attcctgaga
     3121 tggccaagcc agcaaacatt gagatgatga ttgttaccaa gtgtttgcgt aatgtttcaa
     3181 aaatttcttc caagttgatt gtctcttcca ttttttctcc actttcctat ttattctcta
     3241 gattcatttg atcttttaat tcttttttta cacgatcaag ttcttgatca ctgacttttt
     3301 gataggagat attatcttgc ataaagcctt ctccttgcag ctgatcttgc ttgatcgtac
     3361 caaatgcctc ccgataatct aaagcgattt ttttcatttc atcaaagctc atgtctgttt
     3421 tcatattcga ctctagagca ttcaaaacgg attgatagtt gctgacaccg tttaaactca
     3481 atactttttt tgctacacct tctacgatct ttctttgacg ctcttgtcta ccatagtcac
     3541 catttggatc ttcataacgc atacgagaat aagataatgc ctgatccccg ttcaatgtga
     3601 ttttaccgat tgtaaagtca tagccatctt gtgagaaagt gaggtcatta ttgacttcga
     3661 taccaccaac tgcatcaaca agttccttta gccctttcat attgatagag acatagtgat
     3721 cgattgggat gtctacatat ttttgtacgg tatccatcgc catggcagct ccgccgaatg
     3781 cgtaagcgtg gttgatcttg tcttttgtgt cacgacctac gatgtcgaca tacgtatcac
     3841 gaggaatgct gacgatcgtt gtctgtttgt cttcaggact gactatggca accatcatcg
     3901 tatctgatct tccttgatcg acccgaccta aatctccagt atcaatcccc atcaatagaa
     3961 cagagaaaga ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct tgggttcgtt
     4021 ccactgattc gtaagttttc tcaatagaat gtgagagatc cagatataat tttgctccta
     4081 ctcctacaat tgctaatact aatacgataa gaactgctaa aataatcaga aatatttttt
     4141 tccctcttga ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa ccttgcttga
     4201 atcaacgttt taactgtcaa taacttatat tgctatagcc catttgctga gcaacagttt
     4261 gtgcttttgt tgcgtatgct gcgcttattt tttcattccg gatctgctct atttgactga
     4321 gcgccgtcaa atattgatcg taagtagcgg tggcagtcac ttgaccatcc ttcaacatcg
     4381 tatcgaataa ctgatctagt tgcgtgactt gcgcgatctg agtatcagcg aatcctaaat
     4441 attgcataac aaccgttttc caaggactat ctttaaaaaa gctcatattt tcacgtacac
     4501 gagcgacatc tgcacttgtc accttttctt tgatgatgac atcgtctaca gcttgctgcc
     4561 agtttgatac atttttagta aacaaatcgt tgacagctgt ctggatcttc agttttgatt
     4621 ctgcactatc taactgtgtc acgattgctt tcttctcatc ttggattgca acagcttctt
     4681 taggcaattc tgattctttg atgtcgtaat catctgctga aagtttgaca taggatagct
     4741 tatttgacaa acttgtgatt gtttcttctg ttatttcagg gtttagaaaa attttttctt
     4801 ttgtactata cagcttgtct acttcttttg ataaagaagt caaggtgtct ttttgattgg
     4861 tgatggcaga ttctgcataa ttgatccgtt gctgatgttg ccgctgatta ttgatatggt
     4921 aaccaaacaa acagatcgca atagcacctg cacctaccgc cacagcagtc aatgacttga
     4981 cgaatggctt ttccatagat tcactcctaa gtattgatga tagataaatt aagatataat
     5041 tcacttacat aaaaatgaca gctttttatt ttaaaaaaca taattaaact aatatattca
     5101 cagaaatatt tgtaaacaag tttt
//
LOCUS       DAHHPA010000139         5008 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.145, whole genome shotgun sequence.
ACCESSION   DAHHPA010000139 DAHHPA010000000
VERSION     DAHHPA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.145"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..224)
                     /locus_tag="NBH55_002636"
     CDS             complement(<1..224)
                     /locus_tag="NBH55_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1510562.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFL"
     gene            complement(212..589)
                     /locus_tag="NBH55_002637"
     CDS             complement(212..589)
                     /locus_tag="NBH55_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1510563.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            813..2006
                     /locus_tag="NBH55_002638"
     CDS             813..2006
                     /locus_tag="NBH55_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1510564.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(2172..2594)
                     /locus_tag="NBH55_002639"
     CDS             complement(2172..2594)
                     /locus_tag="NBH55_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510565.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            3184..3639
                     /locus_tag="NBH55_002640"
     CDS             3184..3639
                     /locus_tag="NBH55_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510566.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(3801..4973)
                     /locus_tag="NBH55_002641"
     CDS             complement(3801..4973)
                     /locus_tag="NBH55_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCD1510567.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         1457 a          940 c          914 g         1697 t
ORIGIN      
        1 agaaaataac ctgcttctgt caagatcatt ttgcggtttt cgcgtacaaa cagttctgtt
       61 cctagttcct cttctaactc cttcagttgt cggcttaagg ttggttgtgt aagatgtaga
      121 acttctgcgg cttgagagat tgttccttct tctgcaattg tccaaaaata ttttagtaag
      181 cgtaactcca tagcgatgct ccttgatttt tttacttgat acattccatt ttatttctag
      241 ataacacacg atcatatttc ttgatttttc cttgtaaacg ttccaaacat tcttgcattt
      301 cagcaatttt tttcgtcaaa agttcacttt cttcaatcaa aagcttacgc cgctcaaata
      361 cagttgcttc tccttcgcta gataggcgaa tatattcaat caaagactcg atcgataatc
      421 cggcatttcg catacagatc gtaaagtcga cccattttag gtcttcttct gtataatcac
      481 ggattccgcc ttttgtccga ttaactggtg gaatcaaccc gattcgttca taatagcgca
      541 atgtatcggc cgaaatgccc ttttcttcac tgacttgttt gatattcatc tgatcactct
      601 ttctcttaat ttcttgccac tttacttatg tattttattt attctacaac tttaagtgta
      661 ctccaagtca atactcattt ctattaaaac taagctggat tatctatatc ggttgtttgt
      721 gtattatttc aaataaaaaa gtcttgtttt tttattcttt tttggatata cttttaacaa
      781 tttagggaaa ttttaggaaa aggggtataa aaatgacaac gtatcagcga aagttattat
      841 tatcgacatc ggcgggaatt gctttagaga atatggatat tatgttttta gctttttcat
      901 tatcttcaat gattacttct tttcatatta gtggtacgca agcgggtttc atttcaacga
      961 tcactaattt ggggatgctc attggaggta ttttttttgg catcatagct gataaatatg
     1021 gacgtgtgaa agtattttct caaacggtcc ttctgttttc gattgcttct ttattgatgt
     1081 attttgcttc aaacatctac ttggtttatc tctttcgctt tatcgcaggt gtaggagcgg
     1141 gaggagaata cggggcgtgt atgtccttga tttccgaaag tttttcaaaa aaacaaattg
     1201 gacgagcttc ttcggttgct ggtattggtg cgcaggtcgg tgcggctttg gctgcgattt
     1261 tagctgctgt gatcattcca acgtttggtt ggaagatgtt gtatgtcgta ggtgtactgc
     1321 cggtgttgat ggtcttgttc attcgtcgcg gcttgactga accaaaggaa ttcaaatctg
     1381 tacaatctgc cgaaaagaaa accaaactta gcagactgtt tgaatcaaaa catcttgctt
     1441 ggcaaacatg tggccttagt atcatggtaa ctgtccaaat tgccggctat tttgggttaa
     1501 tgaattggct gccttcaatt atgcaaaatc gattagggct ttcggtttct gggtcttcat
     1561 tgtggatggt cagtactatt ttaggcatgt ctttggggat gatgactttt ggcataatca
     1621 tggataaatt ggggcctaaa ctagcctttt ctttgttttt aatttgttca tccttatctg
     1681 tctatctact tgtgttagcc catagccagt ggagtttggt agcagccgct gtgatagtcg
     1741 gctactttat caatggcatg tatggtggat atggcgcagt gattagtagt ttgtatccaa
     1801 cagaaatccg cgcgacggcg aataatttca ttatgaacct tggacgagct gttggtggtt
     1861 tttcttctat cgtcatcggt ttcttgatgg atcattatag tttaactgcc gtaatcttgt
     1921 ttttgagcgg tatttaccta gtcagtctgc tggtcttgac aactcttact ggtgttacag
     1981 cattaaggga aagcttgcag cattgatagt tgagtctgta aatgacaaga aagaggtgtg
     2041 gcaaaagctt gcatctgcgg taataagacc agccaaatca aaaacatgag acactgtttc
     2101 cgtttggctg gtcgtattac ttgatgcagg gcagcttttt caactgctaa tttccttcta
     2161 tctggcggct actattcttt gacgtccgtc atttcaaagt actttactaa taacttagca
     2221 taacgaaaaa ctgtggatac actcgtatat tgagcgtctg cataatcttc caaatttttc
     2281 tcaggatgtt gatacatgaa ttcaatcaaa tgataattat atgattttct gtagaaatga
     2341 agataaataa tgtagattgg gaagttctcc tgcttttcca tagaaaagcg ccgattattc
     2401 tcattcttca aaatgacctt atcaggatat ttttggaaca attcttctaa atctgttaga
     2461 taattcaaga tagacttact tgataccttc aattcgtttt gtagttcttc tattgaacac
     2521 caacgttctg tttcaagcag taaatgaatc attttaattt tcttttgaga aacttttgtt
     2581 aaaaaagtct gcatattttc tcctcctgta tctactttac tatactacat acaagaaaaa
     2641 aaacggatac aaatatcata ttgtcacacc gtcaatagtg cataaacctg ttattatttc
     2701 tctttttttc ctaagtattc tattgatatg aatcatttaa tcgtttatta tataaatgtc
     2761 aataatctaa taataaaata acgtacatat tttattattg catacttaat aacagaacta
     2821 tagcaaaaaa tcccgtgtat caaaacggga ttagattaag tttcctcaat cactattcat
     2881 aacacaatta ttataacaag taagtaaaca attgaaaagg acatttattg aaaacggttg
     2941 ttttttactt aaggaggaaa tttagtgaaa aaattagttg cattgttttt ggtcgcagga
     3001 agtttgttga gtgggtgtgg tgctactacg tctactactc aaagcacaag ttcagctcag
     3061 gcttccagca gcagtaccac gatctccgaa agtacggcag attcatcgaa agaacaagaa
     3121 caaattgaaa agaaaaaaga agaagagaaa caaaaagcgg aaaaagctgc actagaaaaa
     3181 aaagtgacag aagcggaatc tgctttgtca caagctgaag caaatcctac aaaagaaaac
     3241 tatgcaactg cttcagcagc tatccaagcg ctacctgaaa acaaacaaga gttgacaagc
     3301 cgtcttgctg caattgattc cacaatcaaa gcaaaagaag cagaagaaga acggaaaaag
     3361 caagaagaaa ttgcacgaca acaagcagaa caagcgcaac aagaagcaat cgcacagcaa
     3421 caagcagaac aggcgcgaca gcaagcagca gctgaacagg cctctcagca ggcacaagcc
     3481 gcagctcctg agcaaaataa tgaacaaact gtctatgtca caccgaccgg ttcaaaatac
     3541 catacacata aatgtggaaa tggtacgtat acccctgcca ctttaagcga agctcaagct
     3601 cgtggtttaa ccccttgctc caaatgttat ggaaactaat tcaaaagtta tactataata
     3661 ataaaattct tctgaaacat aaaaaaatag aagctggcgt tccagatagt aaaccaaaaa
     3721 tagcagttta cacgtccatt tcgtcagctt ctctcttttt tcatttagtc tgttccctct
     3781 gcttttttgg acatcttcat tcattctttc tcaatcaaat agtacacctc tttttctttt
     3841 cgcagtttat atcttccttt tttcaatttc attgaaaact gtttcgaatg tttatgtttt
     3901 acatggattt taagcaggag ctcctcatgt tgcaagcgat agttcctgcc ttttaaatca
     3961 gaaaatctca ctagtccgta ttcatcagaa gctttcacaa atggaacttt ttctctagta
     4021 atatagtgtt tgccgttgag cgtttttaca gaaaagacca ttccttcgat tggtcgtccg
     4081 tttgtgttct ttacataaaa agataaagga tagtttcgct tagcgatggc caaagaatct
     4141 aaataagcga tgagtagcca aactaagccg attgatccaa gtagtatacc gataaaccaa
     4201 ataaaaaatt tccatttttt ccattgatca attccttgga tcgctttttt tgcctctttt
     4261 gcttgataag gaatacgatg ccccgtcaca agcagtcgat gtgtattgac catataagga
     4321 gtacaggtaa gcaaagtcac gagatctctg cctttttgga tacccaaaga atctacttca
     4381 tcaggtaaga ccacttggat tttttctact tgatacgcca atgtctttcc attgatctgg
     4441 atgaaaaact ggtctccttt tttcaactta ggcaaatctg taaataattt tgcttctggg
     4501 agtccacgat gcccagaaag aacggcatgg gtgtttttcc cgccagtcgg ataagaactt
     4561 tcttccaata atgacgttcc tttttgcaaa aatatttccg ttgtctgatc gaatataggt
     4621 aagctgacat tgatttttgg gatttcgatg actccaagca tatgttccat aaaaaaggca
     4681 ttactaggta aatcttttaa tgcttttgca tcaactgctt ggttgaaact gcctatccct
     4741 ggtgatacgt tttgctctgc caactgttgg tttttctttg tcattttttc ctgctgtttt
     4801 tttatttcct ctgaattctt attcgatgct tgcttttgat aatggatgat catttgctgg
     4861 gcaagaaaat cattcagtga atcttctaca aaaggataag aaaagatccc gatcccaacg
     4921 actaaaatga ttgccatgaa gatgtccagt attcttattc tgcttttatt catcatgtcc
     4981 acctattttc taatatgtat atgaagag
//
LOCUS       DAHHPA010000140         5007 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.146, whole genome shotgun sequence.
ACCESSION   DAHHPA010000140 DAHHPA010000000
VERSION     DAHHPA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.146"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..182)
                     /locus_tag="NBH55_002642"
     CDS             complement(<1..182)
                     /locus_tag="NBH55_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1510568.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="NBH55_002643"
     CDS             517..951
                     /locus_tag="NBH55_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD1510569.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="NBH55_002644"
     CDS             974..1294
                     /locus_tag="NBH55_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510570.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="NBH55_002645"
     CDS             1435..1626
                     /locus_tag="NBH55_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510571.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="NBH55_002646"
     CDS             complement(1697..1954)
                     /locus_tag="NBH55_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCD1510572.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="NBH55_002647"
     CDS             2112..2375
                     /locus_tag="NBH55_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510573.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="NBH55_002648"
     CDS             2399..2587
                     /locus_tag="NBH55_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510574.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="NBH55_002649"
     CDS             complement(2670..2855)
                     /locus_tag="NBH55_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510575.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="NBH55_002650"
     CDS             3070..3441
                     /locus_tag="NBH55_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD1510576.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="NBH55_002651"
     tRNA            complement(3591..3663)
                     /locus_tag="NBH55_002651"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="NBH55_002652"
     CDS             complement(3709..4137)
                     /locus_tag="NBH55_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510577.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="NBH55_002653"
     CDS             complement(4197..4955)
                     /locus_tag="NBH55_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCD1510578.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1743 a          784 c          979 g         1501 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 catgatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt attttta
//
LOCUS       DAHHPA010000141         4998 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.147, whole genome shotgun sequence.
ACCESSION   DAHHPA010000141 DAHHPA010000000
VERSION     DAHHPA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.147"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(18..1784)
                     /locus_tag="NBH55_002654"
     CDS             complement(18..1784)
                     /locus_tag="NBH55_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1510579.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1744..3498)
                     /locus_tag="NBH55_002655"
     CDS             complement(1744..3498)
                     /locus_tag="NBH55_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1510580.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3608..4177)
                     /locus_tag="NBH55_002656"
     CDS             complement(3608..4177)
                     /locus_tag="NBH55_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCD1510581.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4195..>4998)
                     /locus_tag="NBH55_002657"
     CDS             complement(4195..>4998)
                     /locus_tag="NBH55_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1510582.1"
                     /translation="KELIDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVP
                     YIEKVSADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPT
                     KGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKM
                     KDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGA
                     VVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1657 a          944 c          675 g         1722 t
ORIGIN      
        1 atttaaagcc tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat
       61 agtttagttt tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga
      121 gtaccttgat caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata
      181 gacttcaagc gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt
      241 tcctgaatac gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa
      301 attggcgcat ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa
      361 agtgaacttc cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag
      421 tcttcgcagt tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt
      481 ccaatacgta tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata
      541 gcgattttgc taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa
      601 atacagcctt catcataatc atacagtcta gacactaatt tcaagatact agttttcccg
      661 gaaccactag cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga
      721 tctaaaattg gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa
      781 gatttcagag gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc
      841 tgtattttag gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa
      901 tcctttattt ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc
      961 gttaacagta agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata
     1021 gaggataagg ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc
     1081 tcttccattt ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc
     1141 tcttgctgca attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa
     1201 tatttagtat tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat
     1261 aacgttggca aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa
     1321 ataaaaggga aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc
     1381 tcaatacctt caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa
     1441 aaataggata atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt
     1501 tctttataag tactattata taagttttca tactccctat ctaacaacca atacatgaaa
     1561 agcaaagtaa caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact
     1621 aattcgtcta tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa
     1681 aaactagaaa tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg
     1741 tttttataaa aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac
     1801 caattgttta tacacttgat gtttagccat taattcatta tgatttccac gttctactat
     1861 tttcccttct gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc
     1921 tatcataata actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc
     1981 attatctgga tcaatagatg cactggcttc atccataata acaatctttg catcttttaa
     2041 aattgctctg gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact
     2101 accaattaag gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa
     2161 tcttagcgcg tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc
     2221 gtaaatactc tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat
     2281 tgtgtctttt gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac
     2341 attgtagaaa cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac
     2401 taatgcataa attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc
     2461 tttgtatgaa aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt
     2521 cccatatttt agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc
     2581 atatcttgct tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat
     2641 aattccactt aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag
     2701 agaagttata aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca
     2761 ttgaaaaaga acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata
     2821 atcgttaatt gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat
     2881 aacttgaatt ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg
     2941 atatttttga ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac
     3001 taacatggcg attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga
     3061 taaaacaggt gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg
     3121 tgttttagcc gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa
     3181 aaaacgaaaa cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa
     3241 gcgaaaccca aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt
     3301 taaccaagca acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat
     3361 tagagactga agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa
     3421 atcagaaaat aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa
     3481 ttttttgtat actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac
     3541 aactgttaat gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa
     3601 ctacgttcta ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag
     3661 ataaacttgc tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca
     3721 ttgcatccat tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg
     3781 taaagaacca tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag
     3841 acgcaacttg aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga
     3901 tcgccatact aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa
     3961 ataacaataa aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa
     4021 tataaacaaa tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa
     4081 acgccccacc aatactacta accattattg aaaaaaagta aattactagg gaaataactg
     4141 tagctaaacc aataaaaata taatcttttg ttttcatata tattctcctc aattctattt
     4201 cgttatgtca ttaaaaatct gttctatcaa agttgaatct tctactctaa gttttatatc
     4261 tttttttatt ccaccatttt ttaaataaat aattctattg catgttttat aaagaaattc
     4321 cggatcatga gtagctataa ctactgcacc cttttctttt aagtctttaa taaggtcagc
     4381 aatcctttcc atattaatat aatccaaccc acttgtaggt tcatcaaata gaaataaatt
     4441 agtttttgac aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc
     4501 aaacggtaat tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg
     4561 atctttacta actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa
     4621 ttggtaatcc atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat
     4681 tatctttcct ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc
     4741 cacaccgttt ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc
     4801 ttttagaata ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc
     4861 gatataggga acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt
     4921 ttcagttttt aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac
     4981 ttgatcaatt aattcttt
//
LOCUS       DAHHPA010000142         4965 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.148, whole genome shotgun sequence.
ACCESSION   DAHHPA010000142 DAHHPA010000000
VERSION     DAHHPA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.148"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            329..1252
                     /gene="truB"
                     /locus_tag="NBH55_002658"
     CDS             329..1252
                     /gene="truB"
                     /locus_tag="NBH55_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCD1510583.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1256..2197
                     /gene="ribF"
                     /locus_tag="NBH55_002659"
     CDS             1256..2197
                     /gene="ribF"
                     /locus_tag="NBH55_002659"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCD1510584.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2592..3377
                     /locus_tag="NBH55_002660"
     CDS             2592..3377
                     /locus_tag="NBH55_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1510585.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3390..4241
                     /locus_tag="NBH55_002661"
     CDS             3390..4241
                     /locus_tag="NBH55_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCD1510586.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4365..4817)
                     /locus_tag="NBH55_002662"
     CDS             complement(4365..4817)
                     /locus_tag="NBH55_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510587.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1678 a          814 c         1036 g         1437 t
ORIGIN      
        1 attaagaaac tgaaaaaata aataaaaaaa aacggaaaaa agactggaag aatggacagt
       61 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
      121 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaaaacata
      181 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
      241 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
      301 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttaactttat ggaaagaacg
      361 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
      421 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
      481 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
      541 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
      601 agatatgtct ttatctgaag aaaaaataga tgaagtgatg gcttcattta ttggtgagat
      661 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
      721 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
      781 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
      841 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
      901 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaaaga
      961 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
     1021 gctgccaata gaaaaaggag tgaaacgatt caaacgagta gatattactg atgaagtatg
     1081 gcagaaagta aaaaacggta tgagacttaa ttatactgta tttgcgctcc cagaaatgcc
     1141 gtcagaagaa attgctttgt tttataaagg caaagttgtc agtatctatc agccaaaccc
     1201 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
     1261 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
     1321 agtactagga ttttttgacg gcgtgcatat tggacaccag aaagtcataa agacaggtat
     1381 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
     1441 gattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
     1501 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
     1561 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
     1621 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
     1681 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
     1741 aggtgaaaaa atcagctcta cccgtattcg aggattacta gacagcggac aagtaaaaga
     1801 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
     1861 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cagttcattt
     1921 gcctaaagaa ggtgtttatg tatcggaaat aaaaattggg gaaatttggt atccagccat
     1981 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagaattgta
     2041 tattttggac tttaatcaag atatctatgg tgaacatgtc gcagtacgct ggaatcaatt
     2101 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaagagga
     2161 tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg
     2221 tttatgtata actagcaatt tcaataaaat gggtgataaa taagcccagg aaaggggctg
     2281 tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct
     2341 tcggaaataa gccgaaattt cacaaaaatt tgagaagtaa tttttgtaaa ttacttctta
     2401 tttcttgaag ctggccactt ctgtcacaac ctctttcttt atagaaataa aaggacacta
     2461 gcaattcatg aaaacgattg ctaaagcgta tcatttgctt tagactatat atcttggtgt
     2521 acactcactt taagacggaa ataaaagtta tccgtaataa caggtttagg tatgaaaaag
     2581 gagtgggtac tatgtataag gaattagaaa acaaagtcgc agtagtaact ggtggctcaa
     2641 aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca
     2701 actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg
     2761 gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag cgtttacttg
     2821 atacggcttt agaaaatttt gggacactgg acgtttggat taataatgct gggatggaaa
     2881 atcagcatcc aacccatgaa cttagccttg aagattggga aagagtatta aaagtcaatt
     2941 tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag aatgataaaa
     3001 aaggaaatat catcaatatg tcttctgttc atgaacaaat cccatggcca acgtttgctc
     3061 attacgctgc ttcaaaaggc ggagtaaaac tattcaccga aacggtagca atggagtacg
     3121 caccaaaggg aatcagaatc aatagtatag gacctggtgc tatccgtaca cctattaatg
     3181 cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc ccactaggac
     3241 gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca gacgaatcca
     3301 gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat ccttctttcc
     3361 aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg atcccggcag
     3421 ttgggtgggg gatccagcca ctgattttga aaaagattgg tggcagacca acaaatgaga
     3481 tattaggaac tggtattgga gccgtgctcg ttggcttact tgttcaatta ttcgcttctc
     3541 cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca ttctgggtca
     3601 tcggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa acgatgccaa
     3661 tttcgactgc attacagtta gtcggaacct cattgattgg tgtcttcgct tttggagaat
     3721 ggtctggaag tatgggaaaa atcattggtg cagcagcaat cattcttctt gtatttggtt
     3781 ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca aaaggaatca
     3841 gtctattagc atcaacaagt attggttatt gggtttacag tgcattgccc aaactagttg
     3901 atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta ggggcagtga
     3961 tttatgtgat tttccgaaaa ccaaccgcat tttctgaaga caagagttgg aaagcaacaa
     4021 tagttggagc catcttcagt gtgtcagcat tggcttatat tttttcagca aatgaaaatg
     4081 gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca ttaggcggta
     4141 tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt atcggattat
     4201 tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga tggtaaaatc
     4261 tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa aaaatagcgc
     4321 tagaatttcc aacatctctg gaatttctag cgctttttaa ttgtttaaga tacaagtgaa
     4381 atatctgtct gcatcaataa tttttcattg atccattgac taaaatttac gacatgattg
     4441 aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga
     4501 cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc tcggatttga
     4561 ttgattgctg taaaatcacg atactgaagg ttgaaacgca tcgtgattgg tgcatttagc
     4621 atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa
     4681 agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag ttttccttca
     4741 tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa ttcatttact
     4801 gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac ttagttgata
     4861 tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt aaaaatcaag
     4921 tcagaaaatt ctaacttttc tttctctaaa gaaattcgtt ctctc
//
LOCUS       DAHHPA010000143         4946 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.149, whole genome shotgun sequence.
ACCESSION   DAHHPA010000143 DAHHPA010000000
VERSION     DAHHPA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.149"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..863
                     /locus_tag="NBH55_002663"
     CDS             <1..863
                     /locus_tag="NBH55_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1510588.1"
                     /translation="MSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMITS
                     GKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYEG
                     QILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEFV
                     QANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTET
                     EGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLYA
                     DMYKLQSSGI"
     gene            1189..2040
                     /locus_tag="NBH55_002664"
     CDS             1189..2040
                     /locus_tag="NBH55_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD1510589.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2137..4017
                     /locus_tag="NBH55_002665"
     CDS             2137..4017
                     /locus_tag="NBH55_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1510590.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(4057..>4946)
                     /locus_tag="NBH55_002666"
     CDS             complement(4057..>4946)
                     /locus_tag="NBH55_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1510591.1"
                     /translation="KIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYVHPSTL
                     IRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMASLEQM
                     TIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDVTIVTT
                     FGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDSTLSAMS
                     TAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQKFDKR
                     PISSAIMKEPFNFENKEVE"
BASE COUNT         1550 a          863 c         1121 g         1412 t
ORIGIN      
        1 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
       61 gtatcttgaa gattatggat acagaagaat atgcaccaca acaaaaccct ggagcagatc
      121 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
      181 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
      241 gacacaccgg aagtgggaaa agctcgatca tcaatgtgtt gatgcggttt tacgaatttt
      301 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
      361 gtaagaaaat ggggatggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
      421 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
      481 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
      541 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
      601 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
      661 gattgatcca agaaggattg gcgaaaatgc gacaaggacg gacaacgatt gcgattgccc
      721 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
      781 tagaacacgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
      841 aactgcaaag cagtggtata taaaagatgt tgtgacagta gcattttgac ctaagcacta
      901 gagtgaaaat aaaacatagc ttcccatatt tttcatctgt gagagttagt gtcgtagtta
      961 ccagatttaa aaaagacgac gtcagctagt cagaagtgcc taatagcccc taagaaagcg
     1021 ttgacaaaat cgtttgcttt cgataataca aagaaaacga tacaacttgg atagtgattg
     1081 cctaatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
     1141 tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat gctgattgag
     1201 aaagtactga ataacaacgt cgtaataacc agagatgaaa gaaatcagga aatgatcgtg
     1261 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     1321 gacaaaaaat ttcagttgac aaatgacgga ctttcacaac agttccaaga attggcacaa
     1381 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggat
     1441 ctacaagtcc atttgaatga ttctgtgtat attgcattga cggatcattt gtatacagct
     1501 atcgaacgtc aaaaaatggg gatctttgtg ccaaatgtat tgctgtatga tatcaaacaa
     1561 ctgtttccta aagagtatgc cattgggaaa aaaacagtga agaaaatctc ggataaatac
     1621 cacatcaagt tatcagaaga cgaagccgga tttatcgcat tgcatttagt caatgcacaa
     1681 ggcattcaaa gccagcaaga caatacctac ctgtttacca caacaataca ggatatttta
     1741 aatatcgtcc gctattacta taagattaca tttgatgaag attcaatcca tttttttcga
     1801 ttcgtcacac acctacgttt ttttctgaca cgcgtcctag atggagagaa atcagaagtg
     1861 ggagaagttt ctgaggaatt gttccaggtt attcaaaaaa aatataagga cacgtttagt
     1921 tgcattgaga aaatagcaga ctttttaacc ggtgctttcg actaccagat gtccacggat
     1981 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     2041 ataaaaaagg aggacaaatt aacaagaacg tatgaaaata agggtagtga cattcagttg
     2101 gctaaacctt ccactaatta attggaggga atagaaatgg gaaaatacca ggaattagca
     2161 gaaaacatcg taaagaatat cggaggaaaa gaaaatatcc gtagcttgac acactgtgtg
     2221 acgcgacttc gtttcaaact aaaggaggaa aataaagcaa aggatgatgt tttgaaaaag
     2281 atggaaggga tagtcaccat catgaaaagc ggcgggcaat atcaagttgt aatcgggaat
     2341 catgtgccag atgtctatgc agaagtactg acagtagccg ggatttccga tgaagcagaa
     2401 gaagaggttt cttcaagtag taatctgttc aatcgattaa tcgacatatt aagtggctgt
     2461 ttccagcctt ttttaggtgc tttggctgca gcgggaatgg tcaaaggatt aaatgctttg
     2521 ttcttgtttc tcaaatttta tactgaagga tcgggtacgt acatcatgct gaatgggata
     2581 ggggatgcaa ttttttattt catgccagtg atcttagggt acacagcagc gaaaaaattt
     2641 catctgaatc cgatggtggg gctcgtgatt ggtgcagcac tttgttatcc gactgtacaa
     2701 ggagcagcac tacaaagtgg tttccctgat ggcagtcctg cgccttatag cttgctgggt
     2761 cttccggctt atgaaacatt tttgggtatc ccatgggtgg gcagcagtta taccagtagc
     2821 gtcgtacctg ttatcttcat tatcgcattt gccggacaag tccaaaaaat tgccaagaaa
     2881 tggattcctg cagtcgtcca gacctttttg gttccttttt ctgtgttatt aatcgcactt
     2941 cctgctggct ttttgattat cggtccgatt gtcagtatac tgacagatct attaagcacg
     3001 ggattccaag cattgatgga cttttctcca gctgtgtatg gtgcagttct agggtttctt
     3061 tggcaagtac tggtgatttt cggacttcat tggagtatta tccctcttgc gattatgcag
     3121 ctagcgcaaa atgggtggag tcagatgtta gtaggcgttt atggcgcgag ttttgcacag
     3181 acagcagtgg tattggcgat gtatttcaag ttcaaggatc aaaaaatgaa ggcgctttgc
     3241 atgccagctg tgatttcagg aatcttcggc gtaacagaaa cggctattta tggaatcacc
     3301 ttaccaaaga agaagccatt tatttactcg atgattggtg gtgctgtttc aggaatctat
     3361 ttgatggcat ctggaacaat cagctacaca atgggcgggt tagggatttt tggggtggta
     3421 aactacatca cggaaacaga cgccagtggt atgtttcaag cgtttatcgg cattgctatt
     3481 gcttcggtaa tcgggttttc gctgactttt ttcttttgga aggatgaagc aggtgaaaca
     3541 gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
     3601 gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
     3661 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
     3721 ttcccgacga aacatgcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat
     3781 gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga
     3841 gaaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa
     3901 ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg
     3961 gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
     4021 gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa
     4081 attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac
     4141 agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac tatataacac
     4201 gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg
     4261 ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat
     4321 actggtcaaa gctaaaattg gaacatcatt ttgtttcaat atttctgcgg tacgaaggat
     4381 cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta
     4441 cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt
     4501 cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat
     4561 tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga
     4621 aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat
     4681 accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata
     4741 ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt
     4801 tgctacacgg atgagtgtag acgggtggac atagcatcgc tcagcgatct cttggatggt
     4861 catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg
     4921 agaaaaggag atttctttga tttttt
//
LOCUS       DAHHPA010000144         4860 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.150, whole genome shotgun sequence.
ACCESSION   DAHHPA010000144 DAHHPA010000000
VERSION     DAHHPA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.150"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(85..1695)
                     /locus_tag="NBH55_002667"
     CDS             complement(85..1695)
                     /locus_tag="NBH55_002667"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCD1510592.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2889..4229)
                     /gene="glnA"
                     /locus_tag="NBH55_002668"
     CDS             complement(2889..4229)
                     /gene="glnA"
                     /locus_tag="NBH55_002668"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCD1510593.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4257..4649)
                     /locus_tag="NBH55_002669"
     CDS             complement(4257..4649)
                     /locus_tag="NBH55_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1510594.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1433 a         1046 c          797 g         1584 t
ORIGIN      
        1 aacaaatcag aaaaagagaa aagtgacaag aaaattgctt atccccttct cttgctactt
       61 ttctctttct atcaagttca aaaattattt tgtttcgtga tctgctaatg atgctccgac
      121 aaatgctttg attaattttt gcgggcgatt tggacgagaa atcaattctg ggtggaattg
      181 gcatcccaca aagaattttt tctctggcag ctctacgatt tctactaaac ggttatctgg
      241 tgagacacct gaaaagacta gcccttgttc ttcaaacaat tgacgatatt tgttattgaa
      301 ctcatagcgg tgacggtgac gttcttgcac aacatcggct ccatcatatg cttcagccgt
      361 cttcgttcct tttttgattt tacatgggta taatccaagg cgtaaagttc cacccaaatt
      421 ctcgatgttt tcctgatcag ccattaaatc aataatattg tttgctgtat ctggattcgt
      481 ttcagctgat gacgcatctt ctaatccaac gacgttacgt gcaaattcga cacaagccat
      541 ctgcatacca agacaaatac ctaaaaacgg aacgtcattt tcgcgagcat aacgaatcgc
      601 ttcgattttc ccttctactc cgcgatcacc aaatccacca ggaaccaaga ttccatctgc
      661 gtctttcaaa cgttcttcta cattttctct cgttaattct tgtgctttga tccaatcgat
      721 ctcgatgtct gaattatagg cgaaaccaga atgtttcaat gcttcaacta ctgagatata
      781 tgcatctggt aactcgacat atttaccgac aagcgcaatc cgtacttttt tcttcaagtt
      841 caagacacgt tcttctaatt ctttccattc tgtcatgtct gcttctggtg tatctagttt
      901 taaatggtcg caaacaattt gatccattcc ttgtgcttgt aaaactaatg ggatggagta
      961 aagtgtatct acatctggcg actcgataac tgcttctggt tcgacatcac aaaattgtgc
     1021 caatttgttt ttcacgcttt gagaaacagg ttgctctgta cgtaccacca aaatattcgg
     1081 ttggatacct aaaccacgta gctcttttac actgtgctgc gttggtttag ttttcatttc
     1141 tccagcagct ttcaagtaag gaatcaatgt tgtatggata tacataacat tatctgtacc
     1201 gacatctgct ttcatttgac gaagtgcttc aaggaatggc aaagattcta tatcccctac
     1261 tgttccccct acttctgtaa tgatcacatc tgaatcagtc attttagctg ctcgcatgat
     1321 tttttctttg atctcatttg taatatgagg aataacttga acagttgctc ccaagtattc
     1381 gcccttacgt tctttacgca aaacttcaga atagattttt cctgtcgtca cgttggagta
     1441 tttgttcaaa ttgatgtcaa taaaacgttc gtagtggccc agatccaaat ctgtttcagc
     1501 accatcatct gttacgaata cttctccgtg ctgatatggg ctcatcgtcc ccggatccac
     1561 gttgatgtat ggatcaaatt tttggatagt tacttttaag ccgcggtttt tcaataagcg
     1621 tcctaaagaa gctgcaacaa tcccttttcc gattgatgaa acgacaccgc cggtcacaaa
     1681 aatatatttt gtcataagtt caaaaactcc tttttaattt atgtgcagga aaagtctcag
     1741 acaataaaca aagctcccta ttcttcaata agaataggga gccgaaattt ctgtaaactt
     1801 ctgaccctct aaaagggtgc ccaaggtgta taatacaagc tgactaaggg aaagtcaagt
     1861 actcaaatga atactatccg cttacaattt tttctttttc gatatccttt gagaaattat
     1921 ttcttaagtc ctttatgagt gcaaaaaaaa atttaaccca cataataagc ttgatataaa
     1981 catagctaac gttaaaccta agcaaatcag aaaatcgtta ctgaaaaatt ttaagtgaaa
     2041 taatagataa aaaaataaaa caaacattat gatagataat atcccaccta acaaagaact
     2101 actttttcct acttgcagca acagtttata tagcttatcg tagaaacaat ctttttcttt
     2161 cacccttatt tacctccttt acgtaaaaaa cgtagtccac agttctcgga tttttggcat
     2221 atcttttcta ttaaataata tcaaaaacaa aacaattcca ctaatttttt caaagatgac
     2281 ctttttttca acaaaagtgc cttgtaggga ttcattgaag gtaacgaatc acataagtaa
     2341 aaaataagga gatgcgacaa aagtcgtgcc tccttatttc taaataaacg gtgttctcct
     2401 gccaactcct cggtcacaag tcacaatatc catcaaacat gagaagctgt ttttgaaatt
     2461 atttcttgtg attcggagtt aaatggtagg ctcacaacct ctggataaac ggtattcaaa
     2521 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa
     2581 atttttctta atactcgaaa ctaaacgtct ttttcaaaac ctcttggtaa acggtgttca
     2641 aaagcttgca tctgcggtaa taagttcagc caattcaaaa acaagaaaag ctgtttttgc
     2701 ttggctattc ttattacttg ttgcagggca gctttttcac aacctctcca ctaaacggtg
     2761 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttt
     2821 ccgttagttt ctctaatact caggtcaaaa cgctgatttc acagcctcta attcacggta
     2881 ttcctgtttt aataaagttc tagatattgt tctctttccc actcggaaac agtttgtctg
     2941 aatgccgccc attcgagacg ttttgcttca acaaaatttg agaagatatg ttgtcctaat
     3001 gcgtcaatca ttacattatc cttacgcaat tctttgatag cattatggat agttgaaggt
     3061 aagtcctgga tatgtgcagc ttctctttct tcctcatcca ttacataaat gttacggtca
     3121 acagctgctg gcggtgtgat ctgattacgg ataccgtcta gtcctgcttg caacaatact
     3181 gccattgcca agtatgggtt tgcagatgga tcaactgacc gcaattccaa acgtgtggat
     3241 aaaccgcgag attctggtac acgtactaat ggcgaacgat tacggccact ccatgcgaca
     3301 taaacaggcg cttcataacc tggaacgaga cgtttataag aattgactgt tggattgcaa
     3361 acggctgtgt aggcacgagc atgtttcagc aaaccaccta agaaatgata agctgtttca
     3421 cttagttcca attcaccgtc tttatcatag aaaacatttc cttcatcatt gaacaaagac
     3481 atattgcaat gcataccaga accattgatc ccaaataacg gtttcggcat gaaagtcgcg
     3541 tgaagtccat gcttgcgtgc aatcgttttg acaacgagct tgaaggtttg gatattatca
     3601 caagcatcta ccacatctgc atatttaaag tcaatttcat gctgtcctgg cgctacttca
     3661 tgatgtgaag cttccacttc aaagcctaga ctttctaatt ccaacacgat gtcacggcgg
     3721 caattttcac ctaaatctgt tggtgcaaaa tcaaaataac cacctttatc attcaaggtc
     3781 gttgtgatcc caccgtcttc gtctaattta aataagaaga attctggttc aggtcctaaa
     3841 ttgaaagagg taaaaccaag gtcgcgcata tctttcaatg ctcgtttcaa attcccgcga
     3901 ggatcgccgg caaatggtgt cccatccgga ttatagatat cacaaattaa acgagcaact
     3961 tttccatgag cactttccca cgggaagatc atccaagtgg agagatctgg atacaaatac
     4021 atatcgcttt cttcaattcg aacaaatcct tcaatcgaag aaccgtcaaa catcatttta
     4081 ttttctaata ctttgtcgat ctggctcact ggaacttcta catttttgat cgtacctaag
     4141 atgtctgtga acatcaatcg taaaaatcgt acgttttctt cctcaatgat tcgtttgatt
     4201 tcttcacctg ttatctgttt ctttaccatg gtatccctct tcctttttta atttattcag
     4261 tcaaagaatt gtctttgcaa tccttgcgac tgataatgac ctgttttgct taacccacct
     4321 tgcgccaaaa tctcatcacg aaaaatttgt cgaacgtcat gatctgtcaa cggcttcttg
     4381 ttttttgctt cttgtttttt cgcctgtgcc atttcgtata cacgtttgat cccagccata
     4441 ttcaatccct ctgaaagata gtctttaatc tcaagcaaca tatcaatatc attcagcgaa
     4501 tacatacgtc ggtttccttc gcttcgttcc ggatgaacta aatcttgttc ttcataatag
     4561 cgaatttgcc gagcagacaa atcagtcagc ttcataactg taccaattgg aaatacggac
     4621 atagatcggc gcaattcctt ttctcccatc agacgtcccc tttcgtttta taagttggct
     4681 aaagtgtacc aattaaaaaa aatattgtca actgcatatg ttaggttttc taacatgaga
     4741 tctctatttt ttataaaata acaaaaaaat aagaacacaa aaaaggtatc tagttgattt
     4801 tcgtaccaga taccttttat tttttattat actgtactat cctttattag tgtttttatt
//
LOCUS       DAHHPA010000145         4734 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.151, whole genome shotgun sequence.
ACCESSION   DAHHPA010000145 DAHHPA010000000
VERSION     DAHHPA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.151"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(81..1889)
                     /gene="pepF"
                     /locus_tag="NBH55_002670"
     CDS             complement(81..1889)
                     /gene="pepF"
                     /locus_tag="NBH55_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCD1510595.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2065..3063)
                     /locus_tag="NBH55_002671"
     CDS             complement(2065..3063)
                     /locus_tag="NBH55_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCD1510596.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3289..3942)
                     /locus_tag="NBH55_002672"
     CDS             complement(3289..3942)
                     /locus_tag="NBH55_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCD1510597.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="NBH55_002673"
     CDS             complement(4274..4672)
                     /gene="spxA"
                     /locus_tag="NBH55_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCD1510598.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1389 a          996 c          742 g         1607 t
ORIGIN      
        1 aaaaaaatac gtcgactccc ttacaagaga gccgacgtat ttttttcgtt tctattcgag
       61 aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt cttctaattc atttagacgt
      121 tgttcaaaca tcgacattgc atcttcaata tatgcagcct gtgtcatatc aactcctgct
      181 tttttcatta cttccacagg ataatcgctg ttacctgctt ttaggtaagc caagtagttc
      241 tctagtgcct ctggttcctg gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag
      301 ccagtcgagt attggaaaac atagtaatta taataaaaat gcggtatccg tgaccattcg
      361 aatttaattt ctgggtcttc ttcgactgct ggaccataat attttgcatt caatttacca
      421 taactatcac ttaggtattc actagtcaat ggcacacctt tttcatcttc cgtatgcatg
      481 aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag
      541 taatggttga gtacataagc ccgtacacga gggtcttttt ctgtttccaa taaatactcc
      601 gttaggatat tttcattcgt tgtcgaagca atttctgcta aaaagatgga gtagtcgccg
      661 tacacataag gctggttcga acgagtgaaa taactatgaa cactatgtcc catttcgtgg
      721 acaagcgtaa atagctgatc cagcgtatca tgccaattca ataaaatata tggatttgtg
      781 tcatagcttc ccgaagaata agcaccgctt cgtttccctt tattttcgac aacatcgatc
      841 caacgttcag agaatgcttt ttctacgatg gccatgtatt cttcacccat tggtttcagt
      901 gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg taattggagc ttcccctaag
      961 accggtgtat aaagatcata catgtgcagt ttttccactt ctaataaacg tttccgtaat
     1021 tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat
     1081 acactttcag gaatatgatt attgctcaaa gacgcttctc tggcagagct gtaattacgg
     1141 actttcgctt taaaattatg tccttttata tgtgtgccta aagtagaagc aaatgtattt
     1201 ctaaattgtt cgtaaacact gtacaacccc ttaaatgctg cttcgcgcac ccttcgatcc
     1261 gtgctttcta gcaactgacc atacacgcca tgagataatt ggactatttc accattttct
     1321 ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa cagcaaatgt atcacttgaa
     1381 gcatcaaaga tttcacctgc tccagcaaga agagattcct gctcagcaga taagacatgg
     1441 gctcgattat ctactatttg ctggatataa tggcgataga cttccaattt tggttcttct
     1501 ttgaaatatt gccaaatctg gtcatctgac aattgcaata tttctggttc aaaccaggaa
     1561 acggcttcac taactttcga aaacagactg cttgctcttg cataaagcgc ttggtaatct
     1621 gtatttccag tgtcttgatc gtttttaagg tgcgcatata cataaatgac ttcagtttgg
     1681 cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt
     1741 gttcctttgt gtttttcaga ttgttttaac tcttctgata attccaaata tttctcatca
     1801 aactcttggt cgcttgaaaa gatcttggtc aagtcccaag ttaaattttc aggcaattct
     1861 tctcgttttg gcaactgctt tacttccatg aaatccactc cttttcttcc cttagctcta
     1921 tctatcaaaa tagatcatag tctaacatcc acatgtcaca gcatctaact tttctactca
     1981 ttcatgtgtc tgcttccatc aattttagca taactaaatc ttgttttaca tagaaaaccg
     2041 attcctagcc tatttttata cttactattt tttcaaagaa gacaagtgta tgcattcgtt
     2101 aaaaaactgc tgatagacca tctcttcatc aatcatagga aaaagccatt cccttttatg
     2161 ttcttttatt tggcaaagcc atgagttgaa atcaggaact ttctggtttt ccaaggcttc
     2221 ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt
     2281 ttgatagatc cacgatgata gatgcaaaag atgtttatga cgcaagtagc attgttcttg
     2341 tatggatact gtattctttt gcttgtgaat aagttggcgt gtcagccatt tcttcccatc
     2401 ttcactcact ttttctattt ttttcatttg gttcatttta atttcgttac tagtgaatat
     2461 ttctttaagg tccctactag aaaaagtcca gcagctactg caaaaggaaa tatgccctga
     2521 tacagtctct gtaatatgat gatacaaata aagcttttgt ttcttgagat cagctttcca
     2581 gcaatgcact cctctttttc tattatagta gcagcaactt ttttctatca atgagaattg
     2641 cgattgtcca agaaaagaat taccaagtat ccaccatact tggtaactgt tattcaaata
     2701 attttgggtt ctttcaataa atctctggtg agacagtcta gaacattgga tttctattgc
     2761 tagattcgcc aacaaaatat ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa
     2821 tactggttct actgactgtc ccagccaatc catgaagcat tcctttaact gtaagtgctc
     2881 ttttgattct ctttcactaa agaccgaaca gttatctgat ttttgatggg caaaatgagc
     2941 acattttttc tttccctttt tcaaaatcac tctttttcga cagccacgac aataatattt
     3001 ctttcctttt tctgcatgtt tagcaaaaca gcacttatta tattcattca ttgcaagcag
     3061 catttttctc cctctgaact tatttacgtc taagaattta tttacacgaa aaaagagggc
     3121 gtgaaaaagc tattctgcat caagtaataa gaacagctaa acaaaaatag ttcccaatat
     3181 ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa
     3241 aaagactgcg acacaatttt tgtcacagtc tcttactata atgctgattt atacaaaata
     3301 aaatttcgtt cgctcgatag cattacgcgc catcagacat tgtccatatt cattcaatgt
     3361 ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt cgagcaatct gattctctac
     3421 atccgcttgg ttgagatttt ccatccagaa gtgaacgact aggtaatagc gctcttcgaa
     3481 taaatacaaa tcagtccaag cagactctag ttcgacatct gttgcaagtt ggatcacttg
     3541 ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt tcttctaggg cgcttccaga
     3601 taattcttcc tccggatcgc tgccaataat ctgctgacga agaaagtcac tcacatcttc
     3661 tgaatcaaca gtagaatctt ctgattgaga aatttcttca tccggaagat ttttactgat
     3721 aaacagttct aatccgtctc cttttggcaa aacttggaaa gtgacagctt cgcttcctcg
     3781 gaactcctct tcgatatcaa cttcttccaa aatactataa aagaaatttt cgatttcttt
     3841 gtgattgcca agtaaatcca aaaatgaaac gccccgttcc tctaaatctt catgaccaat
     3901 tagtacacgg atcgtatttt cattaatatg ttccatttcc attttgctac acctcacttt
     3961 gccacaatgt ttaatataga ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa
     4021 tctcgctgtt aagcgaattt aataaaacca gcggattttt caacctttca agctatctta
     4081 tttaactata acatttttgg cagacgaata ctatatagcc gacttttcag ctaattttcc
     4141 ctctctattt ttcgtgcaat catcatcttc tactaccttt ttatacatct aatcaagaat
     4201 actttttgta aaaacaaaaa aacttggaaa aactatcttt gtaatagtct tttccaagtt
     4261 tcgtattatc cttctataat ccagccatca attgtgcttg acgtaattct aattgtcgta
     4321 catcgcgcgg taggaaccga cgaatttcat cttcattaaa accaacttgc agacgtttat
     4381 catcaatcat gattggacgg cgaagcaatc caggattgtt ttgcacaagt tccaataatt
     4441 cttgtaacgg cagatcatct aaatccatgt tcagtttttg gaatactttg gaacgtgtcg
     4501 agatgatttc ttctgttccg tcttctgtca tttgtaaaat cgcttttaat tcagaactgt
     4561 tcaatggttc tgaaaaaata ttacgttcaa caaatggaat ctgatgttct tgcaaccacg
     4621 cacgagcctt tctgcaggaa gtacaacttg gggaagtata taatgttaac aacgtgcacc
     4681 actccttttc gtgataagaa tcactttttt gtaacagaag atcataataa tctc
//
LOCUS       DAHHPA010000146         4677 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.152, whole genome shotgun sequence.
ACCESSION   DAHHPA010000146 DAHHPA010000000
VERSION     DAHHPA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.152"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..790
                     /locus_tag="NBH55_002674"
     CDS             <1..790
                     /locus_tag="NBH55_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCD1510599.1"
                     /translation="SISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSK
                     FAADYIQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGST
                     RLVQKAAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTL
                     SSIDSVEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIP
                     KRIGICKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            complement(1087..1635)
                     /locus_tag="NBH55_002675"
     CDS             complement(1087..1635)
                     /locus_tag="NBH55_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510600.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1636..2493)
                     /locus_tag="NBH55_002676"
     CDS             complement(1636..2493)
                     /locus_tag="NBH55_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCD1510601.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2779..3066)
                     /locus_tag="NBH55_002677"
     CDS             complement(2779..3066)
                     /locus_tag="NBH55_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCD1510602.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3056..3385)
                     /locus_tag="NBH55_002678"
     CDS             complement(3056..3385)
                     /locus_tag="NBH55_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD1510603.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(3462..3734)
                     /locus_tag="NBH55_002679"
     CDS             complement(3462..3734)
                     /locus_tag="NBH55_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD1510604.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3734..3997)
                     /locus_tag="NBH55_002680"
     CDS             complement(3734..3997)
                     /locus_tag="NBH55_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD1510605.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(4103..4519)
                     /locus_tag="NBH55_002681"
     CDS             complement(4103..4519)
                     /locus_tag="NBH55_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510606.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4525..>4677)
                     /locus_tag="NBH55_002682"
     CDS             complement(4525..>4677)
                     /locus_tag="NBH55_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCD1510607.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1379 a          895 c          647 g         1756 t
ORIGIN      
        1 tagtatttca attaattctc cgtatgctag gaattatgag ctagaagaaa tcttcaaaaa
       61 ttactttaaa acgaatatct atatattaaa aagtagtgaa gacttatcac aacacgattt
      121 attattttca aaatttgctg ccgattacat tcaagaatta attaagaaag ccaatgtggt
      181 tgcactatct tggggagata ccgtttacaa agtgattgat cagtttaaaa tgggtgctcg
      241 tgaagaatta atttttactc aattcattgg cgaaatcgga aaatataatt ccttagctgg
      301 ctccacacga ttagttcaaa aagctgctga acgttatgag tcttcttatt tgactctcag
      361 tattccttta tatgtcatca atgaaactgc acgtgaacta atggctctag aaccttcttt
      421 agctaatacc ttagcaaccg cttcgcatgc agatatgtta atttctggaa ttggcacctt
      481 atcctctatt gattctgtcg aaccctggaa caagcatcgt aaaatcctat ttccaagcat
      541 gacggatact gttggatttc tttacggaag acccttcaat atcaatggtg aattcatcaa
      601 agcatctgag gataagactt ttggtctttc tttaaaagaa attttcgata ttccaaaacg
      661 aattggtatt tgtaaaagta agttcaaagc ggagagttgt ttaggcgcct tgaatggtaa
      721 tttttttaca gatttatttt tggatgaaaa aactgcttgg aaaatacttt ctttgataaa
      781 aaaagagtga gtcatgtaaa atcctccaaa aaatttagat ttttggtact acttttttgg
      841 agggcagtac aatgattcac tctttttatt ggatatcaaa atcccttttt ttgagaagct
      901 tgtcttgata ttttttgaac ttttccaagc catactccat tacttttcaa gatttaattt
      961 ctttgtattc actgaataat ttccacgttt tttcgttaat gttacttctt attaatttta
     1021 ttgtacttct catgcaatag gatcatatat ccttctaaat cttttttatg ctataaaaag
     1081 ggggttctat ctaactttat tattcattcc ccaccctttg cgcagtgagt cttcggtatc
     1141 ttgttttatc tgactggtag ttcttattat ttctccacgg tcatctaatt ctggaacaat
     1201 tggatatttt tctctgaaca agtctagttc tttcaccacg tcattaaaca aatcttgctt
     1261 atcttcttgc gtaagattct gtttaatata ctcatctaaa gcttttctaa gcaattcatc
     1321 tcttgtcata ttagtctcat acttttctac taaaactctg gaagtcgcat ttaacaaagt
     1381 cgacagtttt aaaggatgct tggtggaata aaccttatca aactttctga ttggtgcgcc
     1441 aacttttttt gttcttcctc tactctgtct ttcaacattg gaggaagttt ttatttcttg
     1501 aacttggtgc acttctaatt gcttattttc agatggtgta attgcttgct gctttttctt
     1561 tgcttctgct tcttttttta ttttggcgag tcctttgttg ttcccaaaaa tatcaagtcc
     1621 tttgatgtca gccaattact ccacctccgc ataccaattc tcgtgttctt ccaattcttt
     1681 caaaatactg ataaataatt cgtggctttt gcgatcccat tgttttaaga gacctttctt
     1741 tgtcttctcc atcgtaatac ctgatccgtc gtatcgtttt aagcgcgcat ttttgagtac
     1801 aacattttta atcaaatgtt ccccataaat ctcttgggct tgtttataca tttcctgatc
     1861 tactgcgtca ccgctttcca acatgaaagg aataacacca gcaacatata aatttgtatc
     1921 aaattctttc acaaaaaagt ccatatactc aagatattgc atcgcatttc tcatcgattt
     1981 tacttgggtc tgtaagacga cgattgccca gtccgcaatg tacatcgcgg atttactata
     2041 tacagaaata gaaggtggga catctaaata gatgacgtca aactcatcaa ataatggttc
     2101 tagcttttct ttcatatatt tgaattggga taatgaatca ttatattggt ctttgatcca
     2161 atcatcatac atttcaaagg acatatctgc tggaagtata tagaggttat tgattacttt
     2221 atagacagcc tctttaggtg gaagattgtt tatcaacacg tcagtcacag tattttcgta
     2281 aacaatcggt tcaccttctc cgcctccttc ttcgttagtg aagttcatat gcgttaagtt
     2341 catcacatca gaagtatttc cttgcatatc caagtcaatt atcaaggttc tttttccttc
     2401 attaactgct gattcaaacc ctaacatttg cgcgcacgta cttttaccta cgccaccttt
     2461 aaaattacca attacatatt tcttcgctac cacgttccta cctcttttct tttagcaata
     2521 ttcatttgat actattataa catatttatt tttagttact accaaaaaaa agatatatat
     2581 gtactacctt ataattaata agtaaaaggt agtacataaa taaggaaaag ttgaaatata
     2641 ttgatttata gctttcctca aagtaatgac gttatttaat ttatcgagaa gcagagaata
     2701 ctcgcgactt tagtcatgta gacgaatcag attttcgata aaaaagaagc cgtccttctt
     2761 aagaacgaca acaaatcact agattaattt tttcaactgt tcataaaagt tttcatgcgt
     2821 tccacataac aatactggaa taaccttgtt ttctataatc tcgtaagcta ctctatatgt
     2881 tgtgcccgca tatttgaacc cactagacca aatacctgat aaatccccag ttttttgttc
     2941 acccttatat ggatcaatcg caatttcatc ataaattaaa ttcaaaaatt tttctttcaa
     3001 tggcttctct tttagctttt ttaaatactt aatgacatgt tttgaaggaa cgatttcata
     3061 agccaatttc tgttgccaac tcctctttag tcattacttt agaattagta agtgtatctt
     3121 cagcaatatc tctaaaggaa gaatgcattt tgttttttct attcttaaat tctacaagaa
     3181 tctcatcttt atcgtagcct tctgcaatta tatcagacaa aatatcttcg ctaaaatcat
     3241 aaaattcttt ttgtggttca acaaattcat aaagaatacc attctctacc aactttgctt
     3301 caacttcgac tccattagga acattaaaat ctttaggtac tgtcaacatg attgaattgc
     3361 cttgttttct tgtttttgta atcatttaaa tccctcattt ctaacagtat gtaattactt
     3421 agtaaataca ttataccacg agataatgta tttacaagta attactgtga ctcgtaatgc
     3481 gtccttgctg aataaatttc aaccacgtgt tcatcttcat ctaccttata aacaacccgg
     3541 tgttgtctat tcaatcttct agaataccta ccttcatatg taggttttaa cttctcaaat
     3601 gattgggtag ggagataggg atcttccttt aaagtctgaa taaccttctc aaattgaggt
     3661 ttcaaattag actgtttgat tttctttaaa tcagctttag ccgagttttt aatcatcaca
     3721 gaatagttat tcattataag ttatcccaat caatttcatc aacattagta aatccactat
     3781 catctttttc tcgcttacga acaacaccca aagtgcctat ttgctcaagt aatagcgttt
     3841 ctttgattgc tttcaaatca tccaaaccaa taagcaccgc attattttca ctgcgctcac
     3901 taatgatttc aatttccgta tgattctcat ttacatcttt caacaactta taaaaatttt
     3961 tccgtgctga gctaggattt aagactgttt ttgccatatc tactccccct ctaatttata
     4021 tctccattct aacgtactta attaagtacg taaattcgat tgttttgttt aaattggagt
     4081 accaaaacag cattgtaaag tgttatccgt ttcctttatt tttattaaca agttttatca
     4141 caggcttcat atatttgatt ataacttcat cttctacgta gatatctgtt atcattacga
     4201 catctgtact atttcgattg agtactttca cttgatctcc agcattgatt ttccctctaa
     4261 atcttttagg aatgaaccaa ctatacatat gattagcatc accaaaatgt gcttttatcg
     4321 cattctcagg atataactta ttcaatagtt cattcttttt aattttcaaa acttcaaatc
     4381 tatacttatc atcatctgct aatctaaata aatcttctaa acgcatgttt tctaactgta
     4441 agttttgaaa atcttccggt ataaattttc ggttctgttg cgaagtttga aaatcaaacg
     4501 cgccctctct gaactccaaa tatcttaggc tgttattccc attaatacct tgatttcagt
     4561 agacaccgaa aagctgaaga gcgttccatt tcttcggttc tttttatata ttcctcgaat
     4621 ggtctccatg cccttaatcg tggaagaggc tgtacggaga ctttgataaa atttatt
//
LOCUS       DAHHPA010000147         4632 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.153, whole genome shotgun sequence.
ACCESSION   DAHHPA010000147 DAHHPA010000000
VERSION     DAHHPA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.153"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(61..2424)
                     /locus_tag="NBH55_002683"
     CDS             complement(61..2424)
                     /locus_tag="NBH55_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HCD1510608.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2741..3928
                     /locus_tag="NBH55_002684"
     CDS             2741..3928
                     /locus_tag="NBH55_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCD1510609.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            4346..4567
                     /locus_tag="NBH55_002685"
     CDS             4346..4567
                     /locus_tag="NBH55_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1510610.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT         1323 a          912 c          889 g         1508 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgattacgt
     2641 ccatataatt gtgtaaaagt gaagcatcaa aaaagccatt gatctcagtt aggagtacaa
     2701 tgttttttgc agaaaacata ctcgaatagg actgatatca atgactcaag tacattttac
     2761 ttttgaaagc gaagaaattc aagcaattat aaacgaaagc ggtgcgaatg ataccgcaaa
     2821 aacacttatg actatcatgt ttaatcagct gatggaagaa caacgaaatc aatatattca
     2881 ggcaaatgcc tatgaacgtt cagaagaacg gcaatcacaa agaaatggtt actacgatcg
     2941 ttcgtttaca actcgaattg gtacgttaga acttcatgtt ccacgaacca gagacggtaa
     3001 gtttagtcct actatctttg aaagatatca gcgaagcgaa aaagctttga tcgctgccat
     3061 gattgaaatg gtcatttctg gtgtctccac tcgcaaagta actaaaacag ttgaactgct
     3121 aacggatggt gcgactgttt ctaaatcatt cgtttcaaat ctgatgaaac agttggatcc
     3181 atttgttttt gagtggagaa accggagcct agaaggctcg gaatatccat ttttcatgtg
     3241 tgatgccctg tatatgaaag taagggaaaa tcatcgtatt gtctcaaaag gtgtatatat
     3301 tggtatcggc attgattctg atggacgacg tacgattctt ggttttgatg ttcaagacgg
     3361 cgaatcggaa gataattggg ataccgtttt tcaatcgttc gttcaacgag gtttgttcgg
     3421 tgtaaaactg gtcatttctg atgcacacaa agggttagtt aaggctgttc gcaagaactt
     3481 tttaggtgcg agctggcaaa gatgccaagc ccactttttg agaaatattt ttgataagct
     3541 gccaaagaaa gtttcttctg atgtaaaaga cgagttgaag agtatcttta aagcctctga
     3601 acttgaattg acacgtgaaa gaaaagagca cttcttggaa aaatatggtt gtgattcaaa
     3661 attaagtgct gcttgcgaca ttttagaaaa cggctttgaa gatgctattc aaattttgtc
     3721 ttttcctgaa aatatccgcc gaaggattcg tacaaccaat gtgttggaac ggttgaacga
     3781 agagattcgc cggagagaac gagtgattcg gatttttcca aatatcaatt ccatcactcg
     3841 gataattgga acacttttga tggaaaaaga cactgagtgg ctggcttctc cacgaaaata
     3901 tttagaattt aattcgaata acatttagca ccatgaacct gtgcgagtct cttctttctg
     3961 aggagagaca tcaagagtcg ctgcgctact gctcctgacc tctctcctca gaaagaaagg
     4021 catggcagtt aggttcacaa cgcaaaacat tgtgcttttg atgaggttga acttttacac
     4081 aagattgtgg acttgacttt catttcgctc ctcatcatgc ataatttgct gaatgacgct
     4141 aggccagtta acacattgtt ggaaaactgg ggctggatcg agcaattcct ttcctgattg
     4201 atccgtttta tgtaaaagtc tctgttatct ttgtcaatat gtgaattggt atcccagcta
     4261 ctatgctaat ttctacaggg attatccaaa atttggatcg tgaccaaatc gaagcggcgt
     4321 aatcggaaat atcaataact tcaacgtgat ttttctttta actgtcaacg gaccgacaaa
     4381 ctcaaacttt tatggtgcag gttccactga tcttctagtt acttggctat acctgactgt
     4441 cactaaaata gattacaatc tagcttccgt tatcggtatc ttgatcttta tctatatgca
     4501 aaaatttata tactatagaa aaaagaaagt cgaatttttt acttttcttt ttctcttttt
     4561 tggttagctg acacgctacc agactaaagt catggaactc ttaggtaata cgtcttaatg
     4621 aaaacaaaaa aa
//
LOCUS       DAHHPA010000148         4550 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.154, whole genome shotgun sequence.
ACCESSION   DAHHPA010000148 DAHHPA010000000
VERSION     DAHHPA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.154"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            564..1475
                     /locus_tag="NBH55_002686"
     CDS             564..1475
                     /locus_tag="NBH55_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HCD1510611.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1962..2228
                     /locus_tag="NBH55_002687"
     CDS             1962..2228
                     /locus_tag="NBH55_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510612.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2300..2641
                     /locus_tag="NBH55_002688"
     CDS             2300..2641
                     /locus_tag="NBH55_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510613.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2696..3115
                     /gene="ssb"
                     /locus_tag="NBH55_002689"
     CDS             2696..3115
                     /gene="ssb"
                     /locus_tag="NBH55_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1510614.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3192..3488
                     /locus_tag="NBH55_002690"
     CDS             3192..3488
                     /locus_tag="NBH55_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510615.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3503..3934
                     /locus_tag="NBH55_002691"
     CDS             3503..3934
                     /locus_tag="NBH55_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCD1510616.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4116..4421
                     /locus_tag="NBH55_002692"
     CDS             4116..4421
                     /locus_tag="NBH55_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510617.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1679 a          714 c          966 g         1191 t
ORIGIN      
        1 acgtggaaaa accgccactt atgagagaaa atggtcaaaa acctaagcgg gaagtctccg
       61 aataacatac caagcgccga cagccaacag ataaagaata cactactaaa aatagtagta
      121 gcataaaatt taaaagggaa aaaatcaacc cctaccccag tcttggggtc tcaaaactaa
      181 ctccccccta accccctctt tcagcctctt ttgaaagagg gggagaatca accccacggc
      241 caacgcccca gtgttcttgg ggctgtccag aacagaaact tgcgagagag tgggggtaaa
      301 acaagtgtag aggaaacttg aagacttgtt cgaacagaaa cttgcgagag agccggagta
      361 aaacaagtgt agaggaaact tgaagacttg ttcgaacaga aacttgcgag agagttggag
      421 taaaacaagt gtagaggaaa cttgaagact tgttcgaaca caaacttaca agagagggga
      481 gtaaagcagt tgcagaggaa tcaggaagat ttgttcgaac agaaacttgc aagaaagcat
      541 tattaaattg aaggtgatga aatatgaagt atttattgca tggctatttt attgattatt
      601 ctactcgcaa tagtagtact ttagcgattc tatgtttgct tcatgaattg agagtggtta
      661 ctagaaaaca gctatacaaa ctattgaatt ttgacagatc aattagtaaa caaggagtaa
      721 cgaatgcttt agtgagatta tatgaaaaac agttaatcga tcgaaaaaaa tatggaacag
      781 aaatgtatta ttatttgacg aaagagggac atcaaagcat aggtgggtat tatacgttgc
      841 cgaaagttcc tgaatataat ttgaatcacc atttacaaat taacaattat ttgattaaag
      901 gaatggcatt ggcaaaagac cacccacatt taaaacggat tatttcagaa agacgtaccg
      961 tctatgaaac gaaggattta aatcctaaga caaaaggaag aaaatttttt gtgccagatt
     1021 ttatttttcg ttttcgagac aaagaaaatc atgagatcaa ttggtccttt gaaattgagc
     1081 taacgttgaa aacacgtcgt cgctaccgtg aagcgatttt tccgaaatat attcgtgagt
     1141 tgcgaacacg tcctgaatca catttaattt atgtgacacc tagtggcgca attcaagaag
     1201 aacttcatgc gtttaagcgt atttttttca agcgcgaaac tgaaaaagat gcgagtcaac
     1261 aagaattgtt tgagcggttg catatttttt cagctttaga gtttgaagag ggattaaaag
     1321 cattattaga gaatgatcca gcagtcaatt ggcggacagt agaacgagaa aaaatttatc
     1381 cgaatggcgc tgttcaaagt cgtttgaatt tatttgaaga atcaactgaa aatgaggaaa
     1441 aaccaaaagt gaagtttacg cctgaagtat tgtaaaagag ggtgttcgct tcgctctttt
     1501 tttgtgccag tttgctggca ccgttcagac gcaagcgtct gaaccggctc gtttttagga
     1561 aggactaact tgtttgattt atccgttgtc aaagaagtgg acgttcgaga gtccctaatc
     1621 gtatagcagt cattcggcga gtataaccta aaacataacc tgccaattgc caagggtaaa
     1681 atgcacgctg cgcgcccttg gcaatcgtca gaacatgttt tcgagccggt tatgccgaac
     1741 gaccgctata cgaaaagggc ctctaaacag aagtccactt tgatccttga caactgaaac
     1801 ccaaaatcaa acaagcaggc cttcgtctaa aaacgagccg aatgaagacc ttgggggttg
     1861 cacccccaaa cccccgtgcc cccgacacac taccgaaaag gtgggggtgg gtggcgaaaa
     1921 aatcaaataa aacattctat tcaagaaagg aagaaacaac catgacaact aaaaacgagc
     1981 aactgaaaga acaggcaaga aaagagttgc aagaaagagg tttgattatt gaggggagct
     2041 ttgaaggaga ttttgaaacc tatattggtt gttatgcaag accgataaac aaacctacag
     2101 ccttagaccc aatgaacgaa caagaagtaa aagaacaaga aaaacatgca gtgaatggtt
     2161 ttccgcaaga tttcactgaa tggtatgagt gggaaattaa caacgggaag cttgaaaact
     2221 tcttataaaa gtcagatgac gtgcaaagag aatgattctc tttgtacggt aaaaatataa
     2281 agaaagaagg aaaaagaaaa tggcaaaaaa cacaccttat caagtaggag acatttttta
     2341 tagtagttgg ggctacgaac aaacgaacgt gactttttgg caaattgtga agttaacaga
     2401 aaaaacagca tggtttcagc ctattcaaaa gaaaacggtc gagcattatt cgtccatgag
     2461 tggaaaagtt gttccaatga aagacaagtt tatagagtat cctttagcaa aaactaaaga
     2521 cagtattgta aaaaaacgaa ttgatccaga aacctatcct gatatgttat gtggttatcg
     2581 tagttgggac acgtttcata gatataaaaa tcaagctgtt tatgaaagta gttattatta
     2641 aggggatagt ttcccctttc aaaaataata aatagaagaa tggagagaaa aaatcatgat
     2701 gaattcagtg aatttagtag gacggttaac aaaagaagtg gatttaaaat acacaccaaa
     2761 aggcaatgcg acaggcacat ttattttagc tgttaatcga aattatacga atgcaaacgg
     2821 cgagcgtgaa gcagattata tccgctgtgt catttggcgg aaagctgcgg aaactttagc
     2881 aaaatatact ggaaaaggaa gtttgattgg cattaatgga cgtcttcaaa cgaggagcta
     2941 tcagaatcag caaggacaga ccatgtatgt tacggaagtt ctagtgacag atttctattt
     3001 gttagagagt aaagaaatca atgagcaacg gtcaaaatca atggcgaata atcaaaataa
     3061 ttcttcaatt ccaggaaatg agattcaaat ttcagatagt tatttgccct tttaagtgaa
     3121 aaataatttt gttctatacc tgatttcttc aggtatagaa caaaaaataa atagagatga
     3181 ggcgattttt tatgaaaatc attgacagtt acgatcaacg aaccatgcaa tatttgggct
     3241 cgtttgatta tacagatgaa aatattatta attatgttgc aagtttacct ccttatcaaa
     3301 gcgttcgctt ggtagatttt gcaagtgatg aattggtatt aacaacgatg ggcaactttt
     3361 tagattatgt cggagacaaa aaatggttgg aaaaaatttt gtctaaattg attgcgaaac
     3421 aaacaggaga agaacccata aatgcgattc aattgttcaa tcgatatgaa caagaaaaca
     3481 atgattaaag aggagagaaa aaatgaaaag attaaaacga agtattttta ttgtgctagc
     3541 attgattgat tttgttggga caggttgtga agcaattgac gttcaaaaag ctcccttatc
     3601 ggaaataaaa caagcaataa caaaacaaga tgaagaacaa ccgctaaata cacaagtaga
     3661 aatagtgaat caaaataaac cgagtttttc tgaagaagat ttttcgttag aaaatggaac
     3721 caggcaattt ttttcagatt tggatcgttt aaatcgggta ggtgtagcaa gtgcactttt
     3781 gcataaaagc aggatgccaa cagaagaacg agaccgatta tacattaagc caactggctg
     3841 gaaacaaaag aaaatgaaga atggcgatta tctttataat cgttgtcatt tgattggcta
     3901 tcaacgtgcg acaagtttcc ccataagtat gtaatacgaa acgggaggat taatcttaag
     3961 attaatcaca actaacctat ttgacagttg gaatgaaagc tatcatgtct gctgaatcaa
     4021 ctggtctgat actcctacat gcaataaagt ctactaggaa gttattgtat gaagctaggt
     4081 aaaggcggca gaacaatttc taagtgtgga atcatatgaa gttggatatg ccgggacgct
     4141 ataaattact cagggtgaga ctgtttttaa ttaaactgac gaacttgcga atgtacgggt
     4201 ctataaccga cttatattcg aaagatataa gattgcgaat gcaaagaaca ttatcgaaaa
     4261 gtaagaatgt tggtcatgaa attcgagatg gaagactagc ggtgtatctg tcaagagttg
     4321 gtaaaacatg tttgattgat tcagatgaga gtgggaatgc gacaaaacgt tttacagaag
     4381 aaatagagca actagcagaa ctgaatatac gatattttta aatactttag cggagaataa
     4441 aaaaatcctc tacagaaaac tacggagggc aacatgtgct attgtttcaa caatacattt
     4501 ttaattttta ccataacaaa aaagtacttg aaaaaaaaca gaaatatgtt
//
LOCUS       DAHHPA010000149         4447 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.155, whole genome shotgun sequence.
ACCESSION   DAHHPA010000149 DAHHPA010000000
VERSION     DAHHPA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.155"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="NBH55_002693"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="NBH55_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCD1510618.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="NBH55_002694"
     CDS             complement(2020..2922)
                     /locus_tag="NBH55_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1510619.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="NBH55_002695"
     CDS             complement(2919..3611)
                     /locus_tag="NBH55_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCD1510620.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="NBH55_002696"
     CDS             complement(3654..4295)
                     /locus_tag="NBH55_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1510621.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1377 a          948 c          768 g         1354 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagatt
//
LOCUS       DAHHPA010000150         4377 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.156, whole genome shotgun sequence.
ACCESSION   DAHHPA010000150 DAHHPA010000000
VERSION     DAHHPA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.156"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(83..1051)
                     /locus_tag="NBH55_002697"
     CDS             complement(83..1051)
                     /locus_tag="NBH55_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCD1510622.1"
                     /translation="MSKNGIIVNEWEKLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1501..2907)
                     /locus_tag="NBH55_002698"
     CDS             complement(1501..2907)
                     /locus_tag="NBH55_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCD1510623.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDIGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            complement(3129..3491)
                     /locus_tag="NBH55_002699"
     CDS             complement(3129..3491)
                     /locus_tag="NBH55_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510624.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3495..3833)
                     /locus_tag="NBH55_002700"
     CDS             complement(3495..3833)
                     /locus_tag="NBH55_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510625.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3812..4231)
                     /locus_tag="NBH55_002701"
     CDS             complement(3812..4231)
                     /locus_tag="NBH55_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510626.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4224..4364)
                     /locus_tag="NBH55_002702"
     CDS             complement(4224..4364)
                     /locus_tag="NBH55_002702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510627.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1235 a          989 c          618 g         1535 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aatttttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactttatt tttttgctct
     1261 tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct atgcaaatat
     1321 ccaaaaacat aaaaccgcac cctttgaaaa gggcgccccc aaacttcgct aacgctaggt
     1381 tggccaaaaa atggactggc cattttttag caaatgtcag cttaaaaggc tagtggttta
     1441 gccgtcaact ccttatcctc ggcacaaacc gcctccggta ccgttgactg cacatcgtaa
     1501 ctaccactcg atctcatctt cagaacgctc taatttatgc tgtgagacgt tttgacgata
     1561 aaataaggtt ttctcctgct ctaatacttc taattcctca gaaagcccta tatccttttc
     1621 tgcaagacca aaacggatag acaaacgagt gatcgatttt tgaaatgcgt tttttaatcg
     1681 ctctaaacgc tggcgtaaag agcgattttc ttctttcagt tttttatttt ccacttgtaa
     1741 atctgctaat tgttttctac cagacaaatg acgctcttct tccaatgtag ctacatactc
     1801 atttaattct gtattttgct caagcatctg taaatattgc tcactcatgt tatcagcatt
     1861 tctcttggca tttgttgctc tcgtagctcg ttgatacaaa tcaacaaaat cattcccaga
     1921 taaatgatac gaaccatcag gaagcctctt gaaactttct aaaggaacca tatctttttg
     1981 gaaaatgacc gccatctctt tttttacatt cagcatattt ttcttctctt cttcaatctt
     2041 cgctctctca gaagccatca gagccttatc acgctcgttt tcttctcttt gcctataaat
     2101 atacctcaac tcatcagaaa cgctctcacg ctcttttaca gccgtttggc gcaattcaga
     2161 aatttcactc atgatttctt tgtattcgtg aatatcttta attttgttgg taccgacaat
     2221 atcccttttc caaccgtaat cattcattaa tcgttctata gagtcaactt cttgatttct
     2281 aaaattattg aaaagcactc gaccattatc tttatcagcg tgtatccctt gttgaaccag
     2341 tgctttatta aagctcggct gcttttgaac accccgttta taaccttcag caaccggcac
     2401 aatattcaag tgcaggtgcg gacttatttc gtcattatga ataacagcat tataaacagc
     2461 caaattaggg tttctctctt gaaactcctt gtaatactga accaaaattt tattggcaat
     2521 cactcgattt tgttcagctt gttgtttcga tatgccagta gaagaaccat ctgctcgatt
     2581 tgtctggaaa tcatctaaaa tgcctatttg cacaataaat tctcgttgag gttccaactt
     2641 tttatccttg cgcactttcg ctaaatagtc ctcgattttt ctatcggctc ttttttgttt
     2701 cgcattatat ttttctacca cttcgccaaa caattcctca tatttatctg ctagattcgt
     2761 ctgctctaaa taaatatttt catccgattt cgtgaagtca atatgcttat gccaatcatt
     2821 ggttttctgg ctatcagtta actcacgatt attatgagaa atacttgtat tcttcgttcc
     2881 ttttttaaat gaaatactaa accccaagtt aaaaccctcc ttcgcgctgt cgctaataaa
     2941 aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta attgtgacat
     3001 ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga caacgaaaaa
     3061 aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc ttcacgcaaa
     3121 ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact ctcactcatc
     3181 tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg ataatctcgt
     3241 tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt ttcgtgttgc
     3301 tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc aaacccaaaa
     3361 cgcttgcgaa gagccttgcc cattgcagca ttttctttct caatcaagcg ttttttctgt
     3421 tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat atctgctaaa
     3481 gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat ttggtttaac
     3541 tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc agttgtttgc
     3601 gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctag atcagcattg
     3661 ataaccgagt tgagaacatc catcattcgt tcaagcattt cactataatt atcttcatcc
     3721 ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc gactgtttct
     3781 tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac cattttcgat
     3841 tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga agcaaagtca
     3901 atttttgttg gtcacgaatc atttccgtta attcagaaat atgcctatct ttttccgcta
     3961 attgttcacg caaaaattca ttttcttttt ctaacgggaa atcattgcct gcatcattga
     4021 ccggtaaatc accagtcaat tgaccacctt ttgaccgata aattgaccgc acaacaccaa
     4081 tcaattcctt gtctaacatg ataaccccat tatctttata ccgagcaagg tcataacctg
     4141 cattgaccag ttgattgcgc actgtatttt tagatatacc caactcatca gcaaggtttt
     4201 ttatggtcat tttttctggc aaatcagtca ttatcttctc cgttactttc agctacccca
     4261 taaacatatt taacagcatg cttagggata atgattgggg aatcaatttt attatttttc
     4321 aaaatcaact catactgcgt tacatcaagt aacttaccgg acattttttt gctatcc
//
LOCUS       DAHHPA010000151         4373 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.157, whole genome shotgun sequence.
ACCESSION   DAHHPA010000151 DAHHPA010000000
VERSION     DAHHPA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.157"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(52..522)
                     /locus_tag="NBH55_002703"
     CDS             complement(52..522)
                     /locus_tag="NBH55_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCD1510628.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="NBH55_002704"
     CDS             complement(681..1139)
                     /locus_tag="NBH55_002704"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCD1510629.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="NBH55_002705"
     CDS             complement(1310..2455)
                     /locus_tag="NBH55_002705"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCD1510630.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="NBH55_002706"
     CDS             complement(2468..3385)
                     /locus_tag="NBH55_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCD1510631.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..>4373
                     /gene="efp"
                     /locus_tag="NBH55_002707"
     CDS             3813..>4373
                     /gene="efp"
                     /locus_tag="NBH55_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCD1510632.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1347 a          815 c          830 g         1381 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaa
//
LOCUS       DAHHPA010000152         4173 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.159, whole genome shotgun sequence.
ACCESSION   DAHHPA010000152 DAHHPA010000000
VERSION     DAHHPA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.159"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            41..742
                     /locus_tag="NBH55_002708"
     CDS             41..742
                     /locus_tag="NBH55_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCD1510633.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1314..2330)
                     /gene="tsaD"
                     /locus_tag="NBH55_002709"
     CDS             complement(1314..2330)
                     /gene="tsaD"
                     /locus_tag="NBH55_002709"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCD1510634.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2327..2791)
                     /gene="rimI"
                     /locus_tag="NBH55_002710"
     CDS             complement(2327..2791)
                     /gene="rimI"
                     /locus_tag="NBH55_002710"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCD1510635.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2798..3340)
                     /gene="rimI"
                     /locus_tag="NBH55_002711"
     CDS             complement(2798..3340)
                     /gene="rimI"
                     /locus_tag="NBH55_002711"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCD1510636.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3324..4046)
                     /gene="tsaB"
                     /locus_tag="NBH55_002712"
     CDS             complement(3324..4046)
                     /gene="tsaB"
                     /locus_tag="NBH55_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCD1510637.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1230 a          889 c          727 g         1327 t
ORIGIN      
        1 tgacaataaa tatagtgaac aataaggaga ggacttttta atgcgtgtta ataaaatcga
       61 ttttcacttt tcaaaactca gatatcaacc tgattttgaa catttctcgg atgaagagtt
      121 ccaacagatc aaggaacata ctgttcttcg ttcttataaa aaaggacaga tccttttcga
      181 cgatggtgat cccagacaac ggttgtatat cttgaccaaa ggactcgttc gtttagaacg
      241 ttatgatgaa agtgcaactt attactacta tgactatgtg aatacaaata acctttttcc
      301 tatgtcagga atgtttggag aagagaccta cacttataca gctcaagcga tgactgatat
      361 tgaaacattc taccttcctg tttcctacta tgaaaaatta gcacgaaaaa atcccgaaca
      421 gttgatctat atgatacaaa aaatttctaa agtagttgaa atacatgaat tgcgtatcca
      481 aatcggattg acctctagcg cctttgaccg ggtaaaacaa aatttattca ttttgaaaga
      541 agaattgggc gtaccaacaa aagatggtca tatcaccatt ccttatccga tcaccttaaa
      601 agaattagca gtgaatagcg gcacgacacg tgaaactgct ggacaagtaa tcaaacaact
      661 acgtgattcc ggactgttag attatgacaa aaagcgtttt actttctgta agacagctac
      721 tactccagct atgagagaat aacgcgaaca aaaataagca gtgaccctga tatttatcag
      781 ggtcactgct tattttcctc cctaaaactt ctgaaagatc cggcagtttt agatcactgg
      841 gcaagactca aagattttct atgtgtgttt cagttatgta atattcactg tttgcaaaaa
      901 atactgatag attaactaga ggaagcgtct tgtcctcatc cgacctagat tattcgatat
      961 tcctcgacaa taaacgatta atcagtgagg gcaatcagag gaacggataa tgatgaataa
     1021 gctgtaaaaa atagggcttt gacaaaagcg tgtcacagcc cttatttcga ttaaacggtg
     1081 tgtacctgtc aactcttcgg tcacaagtca taatgttcat aaacatgagg agctgttttt
     1141 tgaaattgtt ccttgtgaat cagagttaaa cgacaggctt acgaactctt gattaaacgg
     1201 cgttcaaaag cttgcatacg cggtaataag aacagccagg tcaaaaacat gagaagccgt
     1261 ttttcctggg ctgttcttat tacttgatgc agggcagatt tttcacaacc tctttattca
     1321 gataaaacca agcttggatc agcattcaac tgatagctag caaaatttct tttttgcatt
     1381 tccacatgtg ctgctgctcc aatcattgca gcattatctc cacaaagtcg aagaggggga
     1441 atcactagtt ctacctcagg gagtttagtt gccaaagcat cctgcaatcc ttcacgcaac
     1501 ccttgattgg ccgctacacc tccagctaca atcagttgct tgactggata attttgacaa
     1561 gctcgaagtg ttttagtaat caatacatcg atcacacttg cttgaaagct agctgctaaa
     1621 tcatttttgt ctagatcttc tccacgctgt tgtgcattat gcaccaaatt gataaatgca
     1681 ctttttaatc cgctaaaact gaaatcataa ttatcttcat ggatcatcgc tcgtggaaaa
     1741 tggtaattat cttttccctg atgagctagc tgatcgattt cttttccgct tgggtaagaa
     1801 agacctaata ctcttccaac tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc
     1861 ccaatgattt catatgatcc atcttcttgc atatagacta attctgtatg tcctccgcta
     1921 acaagaagtg ccatcaatgg aaactggaat ggtttaacca gacgggctgc atagatatgc
     1981 cctgccatat ggtttacagg aataagcggc aattggtgtg cccaagcaaa agccttagct
     2041 gcagaaatac caatcagcaa cgagccgaca agtcctggtc cataagtgac agcgactgcc
     2101 gataaatctt ctgccgacac accagcttct accagtgcat cttccaagca taaggtgatt
     2161 tgttctacgt gatggcggct agctacttca ggaactacgc caccaaaacg tttatgactg
     2221 tttatttgag aagcaacgat gttagataag atctctgttc catttctaac gaccgctaca
     2281 ctcgtttcat cacaacttga ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct
     2341 ccgtttttaa ctttctgctc ataatgatcg catcttccgt cggattgcga taatatgatt
     2401 tccgtttacc taatatacga aacttttctg attcataaag ttttcttgct gcttcattag
     2461 atagacgtac ttctaaaaaa acatgcagcg aatcttttat ttgttccgta atcagtaatt
     2521 gtaacaaatg ccgtgcatgc ccttttcctt gttctgaaac tgcaacagca atatttgtga
     2581 tctcgacttc atctattact ttcgagtatc caataaagcc acagatctgg ttatcttcaa
     2641 ctaaaagcag ataatcagag tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc
     2701 aaggtgaacc atgtggataa gcattttcgc taatcatcca tacgcattct gccaagtacg
     2761 cttcatcaaa atcttttttt ctgcaaatca actaatctca ctcattttct aaaagtttta
     2821 tcatatccaa cgcatcttct tttgtttcag tgtaatatct ttttttgatc gttgttgctt
     2881 gaaaacctaa cttccgataa agtctttgtg catcttgatt actcatacgc acttctaatg
     2941 acatcgtctc acagcgattc attatagcaa agttctcgat ttcgtcaata agcagcgaac
     3001 cgattcgttt acgctgatat gccggtcgaa cggcaatatt cgtgatatgg caatctaaac
     3061 cgaacgctct tgaaccgata aaaccaatca ttcttccatt atcaaagata cctagatata
     3121 gatgaggaag cggtgacttc aattctgata taaaggcact ttttgtccat ggcaattcgc
     3181 cattatagac ttctcgttcg acatccagca gctctttgat atcttctgta gtcaattctc
     3241 ttagtacgta acgacgatcg atttgttcga ttgttttttc tgtataaatc attttatctt
     3301 gaaaaaagct tctggcatac gctctaaatt ttttcaacat aactttcgtc tccaggtgtg
     3361 tgcgtctcca accatttttc ttctgcttct acccgtttta aatagcgagg aagaaaagct
     3421 tgtttctctg caactggttc agctaaagca cctaattgag ccacagttac accacttgga
     3481 atatcccatg ctggaactgt attaatcatc gcactaggca atatttcctt tatttcatag
     3541 aagaatttgt gacaatcact cccgacgaaa taaatagctt cattcagctt ttgtagttgt
     3601 gtgaacaaat cagaagcaga aatatgtaca tcttcaagta caatttctaa tctattttct
     3661 ttccagcggt atgccccagc gtatacattt tttcttcgag catcaaacat gggcacaata
     3721 attttatcca caccgatgca attagccgcg atggttttca gactagatac tcctactagc
     3781 tctttatcta atgtataagc aatcgttttt gctgtcgtta cacctatacg aagccctgta
     3841 taagaaccgg gaccatcaga aaccgcgatt cggtcaatgt cttttggact tatttgaaga
     3901 tctgcaaaca attggtcgat tgcaggcatc agtgttacac tgtggttgcg tttgatattt
     3961 gtttgtattt gtccaagaat ttgttcgtct tctaccacac cgatcgccaa agtttggttt
     4021 gcagtatcta ttcctaatgt aatcatatat ttgatccttt ctgctatgac attctcgcct
     4081 tccattgtag catacttttt tattctgagg atgaaaatac caaaggcaaa actagtacaa
     4141 tgatacatga ttatctgctg gcgggcaaat ttt
//
LOCUS       DAHHPA010000153         4148 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.160, whole genome shotgun sequence.
ACCESSION   DAHHPA010000153 DAHHPA010000000
VERSION     DAHHPA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.160"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            47..1348
                     /locus_tag="NBH55_002713"
     CDS             47..1348
                     /locus_tag="NBH55_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1510638.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="NBH55_002714"
     CDS             1375..1635
                     /locus_tag="NBH55_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD1510639.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="NBH55_002715"
     CDS             complement(2634..3869)
                     /locus_tag="NBH55_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCD1510640.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1198 a          666 c          862 g         1422 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     1921 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2161 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2281 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga
     4021 aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg ttgtgccaga
     4081 taaatacgag aagttgtgtc attaaaaata aacatgagaa cataaaaaaa taaaataatt
     4141 atattaat
//
LOCUS       DAHHPA010000154         4052 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.161, whole genome shotgun sequence.
ACCESSION   DAHHPA010000154 DAHHPA010000000
VERSION     DAHHPA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.161"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            39..461
                     /locus_tag="NBH55_002716"
     CDS             39..461
                     /locus_tag="NBH55_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510641.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            471..674
                     /locus_tag="NBH55_002717"
     CDS             471..674
                     /locus_tag="NBH55_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510642.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            885..1469
                     /locus_tag="NBH55_002718"
     CDS             885..1469
                     /locus_tag="NBH55_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1510643.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1840..3165
                     /locus_tag="NBH55_002719"
     CDS             1840..3165
                     /locus_tag="NBH55_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCD1510644.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            3158..3508
                     /locus_tag="NBH55_002720"
     CDS             3158..3508
                     /locus_tag="NBH55_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510645.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            3696..3989
                     /locus_tag="NBH55_002721"
     CDS             3696..3989
                     /locus_tag="NBH55_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCD1510646.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
BASE COUNT         1454 a          602 c          781 g         1215 t
ORIGIN      
        1 aaaacgtttt cttatttttg atttcgaggt gatagacaat gaaaaataag tttattttat
       61 ctttaggtat tttaactatt ggaattagtg taactggaac tacaattcct acatttgctg
      121 atagtacaaa tgaaccaact actgtagtag ataataacca agaattaact cctttagata
      181 ggaacggaat agccattggt aacccaacta acgacttaac tgttactgaa aattatttaa
      241 tagtttctcc attacttgct cgagcagctt ctaaaacgat acgagtgaca ggaacgatga
      301 tttttgataa agttggttgg ccttctagac ctcctcaatc acggaattgg aaagaaaaaa
      361 gaaatggtta ttggtactat ggaaaattgt atttacaaag ttataaatca gctggaaaca
      421 attggattgc gacttattca ggaaccttaa ctagaaagta gggatttttt atgccagaat
      481 ttgtgaacac accaaatttt tatcaagata gcgatattct aagtaaaatt aatttaccga
      541 aagaagaatc agctttacaa gttcagcgaa ctttacagga gacacaaaaa attcaaaaat
      601 caatttctaa tctagaagat tctgaagaaa aaagaaaaaa aagagaccaa aaaacatatc
      661 aaaaagaaaa ataatgattt taaaaaaaga gtgtctagtc gacattcttt ttttctaatt
      721 gaaagtgttc ataaaatcac ccatttctgg acacttcctt tttttgcttt atttttcaaa
      781 aaaacaattc atttcatgtt cataaaccca taacaatatc tatgttcagt taaactatat
      841 ggataaagtt atgttacaat aaaattaatg aaggagtgat ttttttgaaa ataggatacg
      901 cccgggtatc tactggttta caaaatttgg atttacaaga agacagccta aataaatttg
      961 gttgtgaaaa aatttttact gatcatatga gtggggcaaa aagtaatcga ccaggattag
     1021 aaatggccat tgattttgtt cgctctggcg atacacttgt cgtttggcgc ttagatcgtt
     1081 taggtagaaa tatggaagat ttgatttcaa tcgtcaatcg actgaatgag cgtggtgtga
     1141 gttttcatag tattcaagaa aatattacga tggacaaatc aagttctaca ggacagctca
     1201 tgtttcattt gtttgcggca tttgcagaat ttgagcgtaa tttgatcctg gaacgatccg
     1261 cagcaggacg agcagctgct cgtgcaagag gacggtttgg tggtcgtcct gaaaaattgt
     1321 ctgaacaaga tttggaatta ctgaagactt tagtggatag cggtacacct attaagacta
     1381 ttgcggaacg gtggaacgtc tcaagaacca ccatttatcg ttatttggca aaaatggagg
     1441 agaaaaatgg aaagacaaat tctcattagt ttttgtcatt taatgctgaa aatttttgcc
     1501 atttatctag aaaagtgaca atagtatgag aattttttgt aagtgtctac tgataactta
     1561 ttatcaaatg ttgtaaggat tttattcatt tgttaatatt aaagaacgga actccaaaaa
     1621 agtccgtact ctaatttgca gtctctcact cttttagagt gagagttttt attagatttt
     1681 caaattaatt agtaaagtga tagaataaat tgatcataca aatcataaga agaagcaggg
     1741 tgatagcata cctatgcttc tttttttatg tatttagcta tttcttcttt acaacatcga
     1801 acatatgttt gtataatgtg gaagaatgga gtgttattta tgatggatta ttcaaaagag
     1861 ccagtaaatg attacttttt aatcgatatg aaaagctttt acgcaagtgt cgaatgtata
     1921 gaaagaaatt tagatccatt aacaacagaa cttgttgtta tgagtcgatc tgataatact
     1981 ggttcaggat tgatattagc ttcttctcct gaagcaaaaa agcggtatgg tattacaaat
     2041 gtgagtagac cacgtgactt accacaacca tttcctaaaa cactacacgt tgttccacca
     2101 cgtatgaacc tatatatcaa gcgaaatatg caggtaaata atattttcag aagatatgtg
     2161 gctgatgaag atctactgat ttactcgatc gatgaatcga ttttaaaagt gacaaaatca
     2221 ctgaatcttt ttacgactga agaaacacga agtcagcgaa ggaagaagct cgctcaaatg
     2281 atccaagaac gtattaaaga agagttaggt ttgattgcta cggtaggcgt cggcgataat
     2341 ccccttttgg ctaaattagc cttagacaat gaagcaaagc ataatgaagg atttattgcg
     2401 gaatggacgt acgaaaacgt tcctgagaaa gtatggaata ttcccgaaat gacggacttt
     2461 tggggaattg gttctcgaat gaaaaaaagg ctcaaccaaa tgggaatttt gagcattaga
     2521 gaccttgcta actggaatcc ttatacgatt aagaatagat taggtgtgat tggattacag
     2581 ctgtacttcc atgctaacgg aatcgatcga acagatatag caatcccgcc agaaccgaca
     2641 aaggaaaaat cttatggtaa ttcacaagtg ttaccacgag attatactag aagaaatgaa
     2701 atcgagctgg tggtaaaaga aatggcggaa caagtagcca ttcgtatcag gcagcataat
     2761 tgtaaaacag gatgtgttca tttaaacatt ggtacgtcga tcttagaaac gagaccagga
     2821 ttttcacacc agatgaaaat acctatcaca gataatacaa aggaattgca gaattattgt
     2881 cttttcttgt ttgataagta ttatgaagga caagaagtaa ggcacgttgg aataacctat
     2941 agcaagcttg tgtacaccga ttcgcttcag ctggatttat ttagtgatcc acaaaagcaa
     3001 attaacgaag aaaatttaga taaaattatt gacaagattc ggcaaaaata tggttttacg
     3061 tcgattgtac atgcttcttc aatgttagaa agcgctagaa gcattactag aagcacactt
     3121 gttggtggtc atgcgggcgg aaatggggga attaaaaatg attgattaca atgatcgtgg
     3181 acgtttaaag tgggcagggt tttatttatc tgatcatact gaagaaattg atgccgacaa
     3241 tgaacaacga accagtcaaa atttagcaaa agaacaaatg acgactgaag agattgcgga
     3301 agtattgaac gctgcagtca taaaaaatag atcagtatct attcaaaaag aagaacgaaa
     3361 cgctgaagga tactatccag aagatattgt tggcaagatc ttaggtagtg atgagctggg
     3421 gatctacgtt ggaaaagaca aaattcacta cgacgaaata cgtcatatcg aattttacca
     3481 ggataataaa tggagtcgat tagaatgaac attatccagc aatatgagct aaagtatata
     3541 acctttgatc agctatcaga agagatttgg ggttatggcc aacttatctc tatgaatcct
     3601 tttttgtttt tacaaatagg agtagatact agaataatgc gtgtgttaaa ctattattgc
     3661 aaacgcaata ataaaagaaa gtaggcttac gcaccatgtc tatcaataca tttgtttact
     3721 cgcacccgat caatgtttac attatcaaga atttaggaat cactgtagaa caattttgtg
     3781 agttatatgc ctacccacaa ggaacggttg caagctggat aacccgacaa cgtagaataa
     3841 aatcattacc tgcttcattt gtatatgatc taagtcttgc ttctagtatg aatatgaccg
     3901 atgtatacga aaagttatta atactagaaa atgaatatga tcaattcaat tcaaatcagc
     3961 agcgaaaagt aaagaaacag atagactaga atagctattt tcgaaaagca atatttaagg
     4021 tataagttat accttaaata ataaaagtat aa
//
LOCUS       DAHHPA010000155         4041 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.162, whole genome shotgun sequence.
ACCESSION   DAHHPA010000155 DAHHPA010000000
VERSION     DAHHPA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.162"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            179..775
                     /locus_tag="NBH55_002722"
     CDS             179..775
                     /locus_tag="NBH55_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD1510647.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="NBH55_002723"
     CDS             899..2698
                     /locus_tag="NBH55_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1510648.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="NBH55_002724"
     CDS             complement(2976..3188)
                     /locus_tag="NBH55_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510649.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="NBH55_002725"
     CDS             3650..3799
                     /locus_tag="NBH55_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510650.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1188 a          688 c          712 g         1453 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat a
//
LOCUS       DAHHPA010000156         3915 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.163, whole genome shotgun sequence.
ACCESSION   DAHHPA010000156 DAHHPA010000000
VERSION     DAHHPA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.163"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(25..1530)
                     /locus_tag="NBH55_002726"
     CDS             complement(25..1530)
                     /locus_tag="NBH55_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD1510651.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1527..2213)
                     /locus_tag="NBH55_002727"
     CDS             complement(1527..2213)
                     /locus_tag="NBH55_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1510652.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="NBH55_002728"
     CDS             complement(2406..3827)
                     /gene="gndA"
                     /locus_tag="NBH55_002728"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCD1510653.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1082 a          837 c          676 g         1320 t
ORIGIN      
        1 aaaaaaacaa gtaaagcaca tgctttactt gtcttctgcc tttgcgatag gaataaagat
       61 tctaaaaatt gtcccttgcc ctactacact ttctgctaag atacgtcctt tatagttttc
      121 aaccagttgt ttagcgatag acaaccccag accatttcca cccttcgttc tagctcttgc
      181 tttatccact cgataaaaac gattgaagat cttattaagg tcttctttgg gtattccttc
      241 gccaaagtcc tgaatcgcga tctcaaattc attcagagtg gaagaaatag agatatggac
      301 ttcttttcgt gtagttgaat actttactgc gttatccaat atgataatga ttaattgttc
      361 aaagtgatta cgataaattt gcagggtcac ttcttttgtc aaatcatcat ctaaagtgat
      421 cacatagtct ggatataata tcttgaaatt attataaact tgataggtga cttcttttcc
      481 tttagttgtt tcattcgcat aatgaacatc tacttgttct gctcttgata agtctagcat
      541 ctcttgaact aagcttttca tacggctgat ttcttgcatg ctggcgctta atgattcttc
      601 taatatctca ggatcatctt tcccccaacg atttagcaga ctaagatgcc cttcaataat
      661 tgcaacaggt gtccttaact catgagatac gtcttccacg aattgctctt gttgttcaat
      721 atacaaacgc atacggtcaa gcatctcatt gaatatttca gctaaatctg ccaattcatc
      781 atttgtgtcg atttcaggca tgtggatatc tgattgcgga tctttacgta tagtatccat
      841 tgtatctctg agtaccttga gtggttttaa gaaataagaa gataaaaaga aacctaaaat
      901 actacttaag atcaaagaaa caacttctaa aacaataaga gtcaataata gcttatttcg
      961 aatatcatag aaagaagata attcatagaa gctttgcgca tagccgatct tttctcttgt
     1021 acctttagaa tatataggct ggatagataa aaagccagtt tttccttcta gtgtaacgat
     1081 cgttggaact ttacgtgtag tctgaatcag gtttcgactg ttttcatggg ttttaaaaat
     1141 caagttctcg tccaaatcat aaacataaag atccattgat ggctgcccta gctcagctat
     1201 aaaggaatcg atcttcatta agtttccttc gaccagtgaa gtatgatcta cgacctcatc
     1261 tccgtcattt gtcgctttcg tcagattacg atacgtattt actaatgtca gttcttcatc
     1321 cgagttagcc aaacgagaag ctacttctga aatcgtacgc tctacattgt ttctttcttt
     1381 tgccacaatc agattgatag atgatttata ggtaatgaca gcgaaaattg taaacacaac
     1441 gaatatgaag aaagaactcg caaaggccca cttgattgtc aaggagggcc ctttcagttc
     1501 tcttttttcc gattttttcg gctttatcac gagcgcatca cataacctgt tccgcgtaca
     1561 gtttggatat agctttcctc cccgggaacg tcaattttgt tgcgaagata acgaatatat
     1621 acgtccacta cgtttgtttc aacttctgtt tcgtatcccc acactttatt tagtaaaacg
     1681 tcacgtgcta ataccacgtt cacgttttcc attaaagtaa gcagcaactc gtattctctt
     1741 tttgttagtt caattacttc atttccgcga cgtacgacac ggttttcttt ttcgatagtc
     1801 aaatcgcgat aagtaatagt agtttgttta gccacatttt tatcgccttc aatatcgatt
     1861 cgacgcaaca atgcacgaag acgtgctaat aattcttcga tagcaaatgg tttgacgatg
     1921 tagtcatctg ctccgtggtc aagtccagat acacgatcga tcacagaatc tcttgcagtc
     1981 atcataatga ttggtgtatt tttgacttgg cgtacacgtc tgcagacttc taaaccgttc
     2041 aattctggga gcatcagatc caacaatatg gcatcccact cattatttaa tgccgcatct
     2101 aaaccagttc ggccgttgta gtggacttct gcgttatacc cttcatgctt caactctagc
     2161 tcgacaaatc gtgccaagtt cttttcatct tcaatgatta gaatattgct catttattat
     2221 tttatccttt ctattctact ggctttttaa aaactctatc attataccga taatatgctt
     2281 aaaaatctat agtcatttta tcatggatag caattttaaa aaagagctga agtctactca
     2341 taacaatcga cttcagctct ttcgtcctct ttttattttc tcatttccta acagccgaaa
     2401 gactattatt cttcatggta ccatgagtaa tggaagattc cttctttgtc gacacgctca
     2461 taggtatgtg cgccaaagta atcgcgttgg gcttggatta tatttgcagg cagacgttct
     2521 gctctgtatg aatcgaagta agcgatggct gatgagaaag tcggtacagg tacgccagct
     2581 tggacagcca aggcaacgac gtctctgact gattgctgat attttttcgt gatttcaacg
     2641 aagtaatcat ctagcaataa attttcgatc tcaggatttt tgtcataggc atccgtaatt
     2701 ttttgcaaga atcgagctcg gatgatacat ccagcgcgcc agatttttgc gatttcacca
     2761 aatggcaagt cccaatcaaa ttcttttgaa gcgacacgta attgagcaaa tccttgggca
     2821 taactcatga ttttactgaa atacaacgct tctcggattt tttcgaccaa ttcttttttg
     2881 tctcccgtga actcaaaatc gttcgtacga gacaaaatct tgcttgcttg tacgcgttct
     2941 tctttataag cagaaatata gcgggcaaat acagattcag tgatcaatgg aagtggcaca
     3001 cctaagtcaa gcgcgctttg gcttgtccat ttacctgttc ctttattccc agctgcatca
     3061 aggatcacat ctactactgg tttacctgtt ccttcatcgt ctttacgtgt cagaatgtca
     3121 gcagtgattt ctatcaaata gctgtctaat tcgccttgat tccattcttt aaagatatca
     3181 gccatttctt ctactgaaag gtttaaaata ttcttcatta agtcataaga ttcagcaatc
     3241 agttgcatat ctccatactc aataccgttg tgtaccattt tcacataatg tccggctcca
     3301 tttgggccaa tatatgtgac acatggtgct ccgtcttctg cttttgcaga aatttgctct
     3361 aagattggtg caactagcgc ataggcttct ttttgtccac caggcatgat tgaaggtcct
     3421 tttaaagctc cttcttcacc accggaaacg cctgtaccga taaagttgat gccagagttg
     3481 gctaactctt cattacgacg catcgtatct ttaaagaatg tatttcctcc gtcaatcaaa
     3541 atatcgcctt tatcaaggtg aggaagtaac tcttggattg ttgcatctgt tgccggtcct
     3601 gctttgacca ttagcaagat ccgacgtggt ttctcgatag attctacaaa ttcttcgatt
     3661 gtatatgttg ctttgaaatt tttatctggg tgttcttcta ctacagcggt tgttttagcc
     3721 cctgtacgat tgaataaagc cactgaatag ccgcggcttt ctatgttcaa ggctagattt
     3781 ttacccataa cggccatacc gacaacgccg atttgttgtt ttgacatatg aactcctcct
     3841 acatacggga tttctcccta atctcttaca ttatagctta acacggctac attgcaaact
     3901 aaagaacaac atatt
//
LOCUS       DAHHPA010000157         3763 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.164, whole genome shotgun sequence.
ACCESSION   DAHHPA010000157 DAHHPA010000000
VERSION     DAHHPA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.164"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            95..592
                     /gene="dfrG"
                     /locus_tag="NBH55_002729"
     CDS             95..592
                     /gene="dfrG"
                     /locus_tag="NBH55_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCD1510654.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(664..2616)
                     /locus_tag="NBH55_002730"
     CDS             complement(664..2616)
                     /locus_tag="NBH55_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCD1510655.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2623..2859)
                     /locus_tag="NBH55_002731"
     CDS             complement(2623..2859)
                     /locus_tag="NBH55_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510656.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3097..3763
                     /locus_tag="NBH55_002732"
                     /pseudo
     CDS             3097..3763
                     /locus_tag="NBH55_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1138 a          664 c          628 g         1333 t
ORIGIN      
        1 tgcttattca actaacgggc aggataatga gaaaaggttt tttgatagaa ataagaaatt
       61 agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat
      121 ggataagaat agagtgattg gcaaagagaa tgacattcct tggaggattc ccaaggactg
      181 ggaatatgtt aaaaatacta caaagggaca tccgataata ttaggtagga agaaccttga
      241 atcaatcgga agagccttac ctgacagaag aaatattatt ctgacgagag ataaggggtt
      301 tacctttaat ggttgtgaaa ttgttcattc aatagaagat gtttttgagt tatgtaaaaa
      361 cgaagaagaa atttttattt tcggaggaga acagatttat aatttgtttt tcccttatgt
      421 tgagaaaatg tacatcacaa aaatacatca tgaattcgaa ggagatactt tttttccaga
      481 agtgaattat gaggaatgga atgaggtatt tgcccaaaaa gggataaaga atgataaaaa
      541 tccgtataac tactattttc atgtatatga aagaaaaaac ttattgagtt aacgggtgcg
      601 ttaattgaag aagaacagta aagataaagc acaagagctt gggattactc caagctcttt
      661 ttgtcatttg aaataaatta tatctttcca atcaaattgt ttatctgtaa tccctaatct
      721 ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa agttgtagta
      781 agttcttaga attgtaatcg caaattgagc atacttcgga ttgaagttag cgtaaatata
      841 gctcttctta tcaccacgag ctgttgttaa tggtctttca agagatgata tataacgtct
      901 tatctgttga ataaagctat tggtcgaatg gtcattaaca tttaatagca tctttgcgat
      961 ttcgtttggt tccagagcag ataaatccgt tctacaatca actgaataaa atcctttatc
     1021 cttagtggct aatggatgtt tgatagggtt atcggcatat ttgcgatatc tttctccatc
     1081 tttactaata gcctcttcgt ggaattgatg acttgttagt aattcagtta gataatgaga
     1141 tgccaaagtc cgtagaaact ttgttttgaa tcctctgata tctccccaat cgagcagttc
     1201 tgcttttgct tgtccgaatt cttttaagca ttgttttctg cttttcgtct tgttaacttg
     1261 gcaaataaaa tgatgtgcat cgctcaacct taattctttc gtgaatactc tataaaaggc
     1321 gtttctaata gagaagtcat catcactaat cattcgccat tcgtcggcat taaccatttg
     1381 tttaattaac caatagtgag cagttgtagt ataaggagca tcaacattta aaccatcgac
     1441 aaatctactc ctattagtaa ttttccctaa ttcgtgaaga taagcattcc tattttcgct
     1501 atcgttagcg gatggttctt gtggataata agaccaatct aacctatcat ttttacgact
     1561 aaaagtattg agatggtctt ctttgaattt tcttgtatct tcatttaatt catccattga
     1621 aatattccaa tcataggcaa catcagaacg gaacacatat cttgataaga cctcagcaga
     1681 tacaactata tacgtttgca tattaaggtc ttcaaacccc gtatatttct ttgaaccttg
     1741 tcctttctta cggacgttat ttaggtagta gtgcatcttg tccgtattaa gccacatctc
     1801 gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt tctaaaaact ccaaacaacg
     1861 cctatacagc cactccagct tatgatagta ggttccaact ccaattccta aaatatcaca
     1921 agtacgactg actgaaaccc ttccaacaac catcttagcg aacattggaa gaatggtgtt
     1981 cttttgttgg tgataagtag tggtttcttc tctttttggt agaacattag tgaacttctt
     2041 acacgcctta cattgatagc gttgagattt gcccctactt ttcccccttt tgtaaaattg
     2101 cttcggctca ttgaatgggg ttgattcttc ttccgagcag ccttctttat gaaattgata
     2161 atctggctca acatcttgga tgctattgat ttctataagg cgtttaattt cttcaacaac
     2221 agaccaatta gataaagggg ttactgtaca gttttgcgat acacctctgc caataggatt
     2281 gtcattgcag tacatacctt tatgaccttt atctttggaa ctaccagtca ttttatatcg
     2341 acttggtttc ccttttacat ctttgaattt gtgctgttct ttcccaaaat tacaacaaaa
     2401 cggattgatg cagtaattat attgaatggg atattcagaa ccgttccatt taaaagacgt
     2461 tggcaagaac aacattttgt tataccttgt tgcaaatcgt tttggagtta ataaatcata
     2521 ttgtttagcc ctatctttta attcttcgtt tgaaatcgga attttaacat ctacaatttc
     2581 atcttccttc gtggctaatc ttttaagctt ggtcattgtc tatcaaccca ctctatcttt
     2641 gatacccatt ttttcaacaa tccgcttatt acttcgcttg ttggcaatcc attcaataaa
     2701 cttcttatcg tctaaaatgt tcaataagaa ttcatccact gcttcgcttt cagtacgctc
     2761 tgcatattca gcgtattgct taactatcac ccttacttgc tctgatattt cccaatccac
     2821 agacttagcg ttcttatttt ttggctcaag aaattgcata ggaaaatgca cccctttttg
     2881 cttagtaaat tgtaaccatt ttaacacacc aatttgcatt cctacaataa ttatttcacg
     2941 ttcgcatttt tatatacatt ttaacttaca ttttgtggtg taaattaaca acaaacgttg
     3001 atttaacaat aaaaaacgag tatcaaattc agttgcaatt tgatactcat ttaggtttac
     3061 attttcactt tttgtactcc aaaactgaac tacttatttt tatttagttg tctctatatt
     3121 ttctctgctt atcggacagt agacatctac tatcgttccg caattgccga agctatcgca
     3181 ataacacttg ttatccctgt attattttat gcttatcaaa tcatctctgg aaaagaagaa
     3241 aagtatccgt ctgtaaagct agcattgagt atgagtttgc tagtgtacag ccatgtacta
     3301 tcaacattaa tgagtacagc attaattatc atttttattt ttattcgttt ggtttctaaa
     3361 ggtttcaaga atgcagattt tattgctata tttaaaaaat tatttagtgc ggcaggaatg
     3421 acattggttc tgacgagtgc tttttggtat ccgatgtttg aacaaatgct ctaccaaaaa
     3481 atcaacaaac ctagcgtgac aaatttatat gctcatgcat ctaatgtgtt cgatagtctg
     3541 actgaagcta tgaacaacga cttaactact tactcaatgg gactagttgg tctgttatct
     3601 ttatgtattc ccctgatttt atttaaaaaa ttaacaaaca tagaaaaaaa gatttattat
     3661 ggtacgtgtt tgacttggct agctacaact agtttggtgc catggtatct tctgcaaaat
     3721 acgccagcca aattgttaca atttccttgg cggatcttaa gct
//
LOCUS       DAHHPA010000158         3735 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.165, whole genome shotgun sequence.
ACCESSION   DAHHPA010000158 DAHHPA010000000
VERSION     DAHHPA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.165"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            119..2560
                     /locus_tag="NBH55_002733"
     CDS             119..2560
                     /locus_tag="NBH55_002733"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCD1510657.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2890..3594)
                     /locus_tag="NBH55_002734"
     CDS             complement(2890..3594)
                     /locus_tag="NBH55_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510658.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYSLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1205 a          653 c          815 g         1062 t
ORIGIN      
        1 atataaaaaa gagaatattt aaggattgtt aaaatttaat cttagaaaaa aataactgga
       61 acaagagcta tttgttttta cagcaaagaa tggtatattt ttgtaggagg tggcttactt
      121 gaaagaagtc gttatgatag atgctgcaag aacgccgata ggaaagtatc gcggaagttt
      181 aagtccattt actgcagttg aattaggcac acttgtgact aaaggattat tagataaaac
      241 aaaactgaaa aaagataaaa tcgatcaagt aatatttgga aatgtcctgc aagctggtaa
      301 tggtcaaaat gttgccagac agattgcttt gaacagcggt ctccctgtag atgtcccagc
      361 aatgacgatc aatgaagttt gcggatctgg tatgaaagca gtgatcttag cacgccagct
      421 gatccaatta ggtgaagcag aacttgtgat agccggcgga acagagagta tgtctcaagc
      481 acctatgctg aaacagtatc agtcagaaac aaatgaatat ggtgaaccaa tttccagtat
      541 ggtcaacgac ggattgactg acgcattttc aaatgcacat atgggattaa ccgcagagaa
      601 tgttgcaaca caattttctg tgagcagaga agagcaggat cgctatgcct tgtcgtccca
      661 gttgaaagca gcacatgctg tcgaagccgg tgtattttct gaggagatca tcccagtcaa
      721 gatttctgat gaagacgtgt tatctgagga tgaagcagtt cgtggaaata gtacattgga
      781 aaaactgggc acgttacgta cagtattctc agaagaagga actgtaacag caggaaatgc
      841 ttccccgttg aatgacggtg cctctgtggt gatccttgca tccaaagaat acgcagaaaa
      901 taataatctg ccttatttag caaccatcaa agaagtagcg gaggtcggta ttgacccaag
      961 tatcatggga atcgctccaa tcaaagccat tcaaaaattg acagatagat caggaatgaa
     1021 tttatcaaca atcgatcttt ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt
     1081 tagccaagaa ctgcagttag atgaagaaaa ggtcaatata tatggaggag caatcgcatt
     1141 aggccatcca attggtgcaa gcggtgcacg tatcttgact acattagctt acggattgct
     1201 tcgtgaacag aagagatacg gtattgcttc attatgtatt ggtggaggtt taggcttggc
     1261 agtcttgtta gaagcgaata tggagcaaac acataaggat gtgcaaaaaa aaaagtttta
     1321 tcagcttacg ccatctgaac ggcgcagtca attaatagaa aaaaatgtat tgacacaaga
     1381 aactgcgttg atttttcagg aacaaacact ttctgaagag ctctccgatc acatgatcga
     1441 aaaccaagtg agtgaagtgg aaatcccaat ggggatcgcg cagaattttc aaatcaatgg
     1501 caagaaaaaa tggattccga tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa
     1561 tggtgcaaaa atctgtggaa atatctgcgc agaaacacct cagcggctga tgcgagggca
     1621 aatcgttcta tcaggaaaat cggagtatca agcagtgatc aatgcagtta atcatcgaaa
     1681 agaagagctg attctttgtg caaatgaaag ttatccttca atcgtgaaac gcgggggcgg
     1741 tgttcaagat atctctactc gggaattcat ggggagtttc catgcatact tatctattga
     1801 ttttttagtg gatgtaaaag atgcaatggg cgctaacatg atcaactcga ttcttgagag
     1861 tgtggcaaac aagttacggg agtggttccc tgaagaagag attctattca gtattttaag
     1921 taattttgct acagaatcat tggcttctgc ctgttgtgaa atcccattcg aacgcttagg
     1981 gagaaacaaa gaaatcggtg aacaaattgc caaaaaaatc caacaggcag gggaatatgc
     2041 gaagcttgac ccttaccgtg cagcaacgca caacaaaggg atcatgaatg gtatagaagc
     2101 tgtagtggca gctacaggaa atgatacacg tgcggttagc gcttcgatcc acgcctacgc
     2161 ggcaagaaat ggattatacc aaggattaac agactggcag atcaaagggg ataaactagt
     2221 tggaaaacta acggtgcctt tggctgttgc cacagtaggc ggagcttcca atatcctgcc
     2281 aaaagcaaaa gcatctttag ctatgttaga tattgattca gccaaggaac tagcacaagt
     2341 aattgctgca gtagggttag ctcaaaacct ggctgctttg cgtgcattag ttacagaagg
     2401 aatccaaaaa ggccacatgg gacttcaagc tcgttcatta gcgatctcaa ttggggctat
     2461 tggtgaggaa atcgagcaag ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca
     2521 gacagctata caaatattgg aaaaaatccg ggaaaaataa aaaagcaata tctttagaag
     2581 aataaagcaa gatttacagt tattttattt actgcagatc ttgctttttt ctctatcaga
     2641 aaatatgttt attattagaa ttttttatag ttaaggttag gcatcttaag aaatcaggca
     2701 actccaagat atcttcaggt agaagagaag tcgcctaaca ttgtaggatc ataggtgatt
     2761 tgttggacag gtcaagctga tatttgagcc tgatgttaga aggaatttct ttttacttgc
     2821 tgtagaaatt attatatgac taaaaaggct gggattttaa atcccagcct tttatgacaa
     2881 atagaatttt tatcgatcag taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa
     2941 agtcaatttg actttgtatt tccgttcgag tgctttttcg ataacgaact cattttcagt
     3001 agtcatagaa atagttgcta atttacgagg ttcatcagca tttttttcat taataactac
     3061 ttcatgttca ttttgttctg tgtaaaattt aggatactca gcaacggctt ttgaaaaatc
     3121 agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt
     3181 tggtttttcc gttttttctt ctggcacact gactgtttct tctgtaactg gtgtcgcaac
     3241 agcttgcgca gttggttcat cgtttttcaa tcgttgatca ttccagtaag acacgatttc
     3301 aataagaaga ttgtcacgga atcgttcgaa aatcccgttt tctcctgaac gaattctctt
     3361 taatacagta ttgaagttgt catcgattgg cacgttgatc cgtgtatctt catttgtttt
     3421 cccagctaat ccgatcgtta agatttgacg gtttttggga tcaagcatga cttgtaacgg
     3481 caaactataa cgtcgcaagc tctcttcttc tagagaacgg aataattgtc ctcttacacc
     3541 atccaatgaa ggacggaatt gatttaataa tttttgtttc tgttttttct tcatatgttc
     3601 tctccttcgc tcataccctt tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat
     3661 gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat ttgcgtattt tcccgttttt
     3721 tgaaagaaaa ataaa
//
LOCUS       DAHHPA010000159         3729 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.166, whole genome shotgun sequence.
ACCESSION   DAHHPA010000159 DAHHPA010000000
VERSION     DAHHPA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.166"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(234..1553)
                     /locus_tag="NBH55_002735"
     CDS             complement(234..1553)
                     /locus_tag="NBH55_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HCD1510659.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1810..2280
                     /locus_tag="NBH55_002736"
     CDS             1810..2280
                     /locus_tag="NBH55_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCD1510660.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2502..3605
                     /locus_tag="NBH55_002737"
     CDS             2502..3605
                     /locus_tag="NBH55_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCD1510661.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         1198 a          638 c          793 g         1100 t
ORIGIN      
        1 tacgcttagt atttattttc acaatattaa cctgaataat tcatagcttt aattctttgg
       61 cacattttat tgaaatatgt atcactaata tccctatcaa ctgattttga ctaaaaagtt
      121 acttcaaaca ttcactgttc aaaaatattg aggaataaat tttgggtgta aggcattttg
      181 agcatacttc tccctggata acttcccagt aaaaaaatcg ttagattgtt ggattagatc
      241 cattgactgg ctcgcctcag gcgagcaaag accctgtaga attcattctg atacacatga
      301 taactagata atttgagata gactcgtcta cccgtttgga ctaatttccc agcaactttc
      361 agaaacttca atcgaataga atgaatggtc attccctttt ggacttcttc aaagccaatc
      421 gttcttaaga agttgactaa gttgtaagct atcaagctga gcatcattcg gacatgattc
      481 tccaaaaagc gaggactgtc tgttttgtca aagtaaaagc cagctttcgc ttctttaatg
      541 aagttctcca ttgtgccacg tttggcatag atagaaaaga tggtatcagg agaaacattt
      601 tctgatagat tcgtcacgat aaattcatgt cgaaagagta gttcgcccgc ttcacgtgtt
      661 gagcgtatac acacgcgacg actttgtgac caggattgtg cttgataagt ggtcgagaag
      721 tactgaactt ctcgttcttc ccacttttga ttatcgccat aaagtactga tttctcagct
      781 atttgaccta gtctacgatt attcttcagt cgaataacat aatgactctt ttttgattca
      841 cacgaatcat acacaccagg tgtagcgaac ccgctgtctc cacgaaccaa gatgtcagtg
      901 tttggtagag agtgattata gtgctctaat aaaggtgtga gaaactcctt tacgcctttt
      961 gatgtatatt gatttcctga acgtagttca gcttttaaga agtcaccagt caatccgtca
     1021 aaagctacca atggatggta accataggtt tggtagtgtg tattataatt cgtttgttct
     1081 tggtgaccaa atgtatctga atgggtcgaa tctaaatcaa tgattaattc cgtatcatta
     1141 cggatgaggc gtgctttatc gataagttct tggttcaaag cttgaagttc atggatattc
     1201 tcctcagata gtcgatctaa aaatcgggaa agtgaagatt gagaagcgag ttctttccta
     1261 tctaaaacag ctttaaacac aggatcttgt ctcaggagat tagctgcaga atcagctgag
     1321 taaccagcaa ttaattgcat aatgaactgc tcaagtatgg ataagttgtc atgagtaaaa
     1381 tacgctcgtt cgtcttcaat gtgaatgtgt tgcttagcca gttcagaaaa accaaagatg
     1441 ttcatcagct ctttcactag gacgagaccc gaatcactcg ataattgacc acctgtatga
     1501 gaaatagtga tatttgaatt gaattttact tggtttttgt gtaagctagt catgaaaaga
     1561 actcctttct tttggttggt ttagtcactt taaccatagc agaaaggggt tcttttttca
     1621 tcacttaacg ggtgaagatg aaaaagtaat tgtaagctgc cgtgaggcag tttcacatgg
     1681 ttttaattaa aagtatgaat tattcaggta gaatctctga agaataaatc acgtgatcgt
     1741 gacgataaaa tacacttacg tcaggtgata aaaaatacat aagtagaaaa ctaagaggag
     1801 gaacagaaaa tgaaagaagt tcaattgatc agaaaaagcg aactatcaga aggtggttgc
     1861 aatgcatgcg gcgtagtaga agcaacaagc tatacattaa agcttggtgc caacaaagcg
     1921 ataatctctg agttgacagt aggcggtctt gtggacagtc tggcgcttgc tgaaggtttt
     1981 attggagaag atatttatga gatgttttca gaaatccgtc agttaaaaaa aggggaaaac
     2041 tgcatagagg ttcatcatga gagcccgaat gtgagattca aacgtggtga taatgagatg
     2101 atcttcaata atcatgtgtc cgatcacacc gagctatatg gaatcgtgaa tcaaatacta
     2161 actgagcttt ttggattggg accttatgcg ttcaaggaag aaaacggtaa tccaaaatta
     2221 aacgaagaat ggcaagaaac gattgaaata caaagaaaca acccgcactt atttcaatag
     2281 gcatccacag tatttcgtaa aagggacttt gacacgactt ttgtcaaaat cccatcgttt
     2341 tttatctatt tgtgttctaa acatttcttt cagagtagta acaactggaa tgactgaaat
     2401 gtaaacagat tatttgcctg tatcaatacg gaagagtaat ataaagtgtg taaacgctat
     2461 catattattc attcaatttt ttatagaggg aaggcattga aatggaaaaa acgtatcaag
     2521 caggtacaaa tgaaggaata gtggatttta tcaatatgga agatttagaa atagccgctt
     2581 cacaagtgat tccagctggt ggttatggtt acatcagcag tggtgctggc gacttattta
     2641 cttatcaaga aaatgaacgg gcattcaatc atcagctgat tattcctcat gtattaagag
     2701 atgtagaatt acccgataca actacacatt ttgacaaaga aacgctgact gcaccaatca
     2761 tcatggcacc agttgctgct catggcttag ctcatgtgaa ggcggagaaa gcttctgcaa
     2821 aaggtgtagc agattttggg acgatctata cagcaagttc ttacgcttct tgtacattag
     2881 aagagataag agaagcaggc ggggagaaag caccgcaatg gtttcaattt tatatgagca
     2941 aagatgatgg gatcaatttg gatattcttg aagtggccaa gcgtaatgga gcaaaagcaa
     3001 tcgtattgac agcggatgca acagttggcg gaaaccgtga aacagaccgc agaaatgggt
     3061 ttaccttccc actgccaatg ccgatcgttc aggcttatca atcaggagta ggccaaacga
     3121 tggatgctgt gtacaaatct tccaaacaga agcttagtcc aaaagatgta gaatttattg
     3181 ctgctcattc tgatttgcca gtctatgtga aaggtgtcca gtcagaagaa gatgtctatc
     3241 gttcattaga atctggtgct ggcggaatct gggtatctaa tcatggtggc cgtcaactag
     3301 atggtggtcc agcagcgttt gattcgttgc aatatgtggc agaagcagta gataaacgtg
     3361 taccaatcgt gtttgacagc ggtgtacgac gcggacagca tgttttcaaa gcaatcgcat
     3421 caggtgcaga tcttgttgct attggcagac ccgttattta tggattgtct ttaggaggaa
     3481 gtacaggagt ccatcaagtg tttgatttct ttaaaacaga attggaaatg gtcatgcaat
     3541 tagctggaac acaaacagta gaagatatca aaaaaataaa attgcgtgag aatcgtttta
     3601 tttaagaaac aaatgacgaa aaaaatagga gtgcggcaga tgatgccgta ctcctatttt
     3661 tatagttcaa ggatgtcact aatagaataa tttgaaacga aaaaataaaa ttgaaatatt
     3721 ttttcatac
//
LOCUS       DAHHPA010000160         3715 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.167, whole genome shotgun sequence.
ACCESSION   DAHHPA010000160 DAHHPA010000000
VERSION     DAHHPA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.167"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..2232)
                     /locus_tag="NBH55_002738"
     CDS             complement(<1..2232)
                     /locus_tag="NBH55_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510662.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="NBH55_002739"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="NBH55_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1077 a          799 c          576 g         1263 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaatatataa aaataacaat aacctttgtg atttttcttg
     3601 tttgaagaat gttagaagaa aaatctctaa atacttataa ttattttata agcaaaaaaa
     3661 tgctatcggt agtatcaccg atagctgagg aaagtcacag tagggactat ttgat
//
LOCUS       DAHHPA010000161         3698 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.169, whole genome shotgun sequence.
ACCESSION   DAHHPA010000161 DAHHPA010000000
VERSION     DAHHPA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.169"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            66..518
                     /locus_tag="NBH55_002740"
     CDS             66..518
                     /locus_tag="NBH55_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510663.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            532..1221
                     /locus_tag="NBH55_002741"
     CDS             532..1221
                     /locus_tag="NBH55_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510664.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1346..3697
                     /locus_tag="NBH55_002742"
     CDS             1346..3697
                     /locus_tag="NBH55_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCD1510665.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1262 a          480 c          771 g         1185 t
ORIGIN      
        1 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
       61 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      121 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      181 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      241 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      301 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      361 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      421 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      481 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      541 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      601 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      661 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      721 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      781 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
      841 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
      901 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
      961 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1021 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1081 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1141 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1201 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1261 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1321 tttggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1381 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1441 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1501 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1561 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1621 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1681 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1741 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1801 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     1861 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     1921 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     1981 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2041 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2101 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2161 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2221 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2281 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2341 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2401 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2461 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2521 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2581 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2641 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2701 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2761 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     2821 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     2881 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     2941 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3001 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3061 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3121 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3181 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3241 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3301 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3361 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3421 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3481 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3541 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3601 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3661 tattcaattt caagaaaata gaagtaaact taattaag
//
LOCUS       DAHHPA010000162         3612 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.170, whole genome shotgun sequence.
ACCESSION   DAHHPA010000162 DAHHPA010000000
VERSION     DAHHPA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.170"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            57..1046
                     /gene="galE"
                     /locus_tag="NBH55_002743"
     CDS             57..1046
                     /gene="galE"
                     /locus_tag="NBH55_002743"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCD1510666.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            1058..2542
                     /gene="galT"
                     /locus_tag="NBH55_002744"
     CDS             1058..2542
                     /gene="galT"
                     /locus_tag="NBH55_002744"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCD1510667.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            2566..3546
                     /locus_tag="NBH55_002745"
     CDS             2566..3546
                     /locus_tag="NBH55_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1510668.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         1223 a          654 c          799 g          936 t
ORIGIN      
        1 ttttaaatta tatagaatca acaaacacaa ttttactaat caaaggagtg ttttttatga
       61 caattttagt actaggcgga gccggttata tcggttcgca cgctgtagac cagctagttc
      121 aaaaggggta tcaagtagct gtggtagata atttgctcac aggtcacaaa caagcagtac
      181 atccagatgc tcatttttat gaaggagaca tccgggataa agaatttcta cgttccgtat
      241 ttgaaaagga accgattgaa ggagtaatcc attttgctgc aagctcttta gtaggagaat
      301 ctgtcgaaaa accattgatg tatttcaaca acaacgtata cggtatgcaa atcttacttg
      361 aagtcatgca cgagttcaac gtgaataaaa tcgtattctc ttctactgca gcaacttatg
      421 gagagccgaa agagtctcca atcacagaag atacaccagc taatcctaaa aatccgtatg
      481 gtgaaagtaa attgatgatg gaaaaaatga tgaagtggtg tgaccaagca tatggcatgc
      541 gctatgtcgc tttgcgctac ttcaatgtag ccggagcaaa agcagatgca tctatcggag
      601 aagatcatac accagaaacg catcttgtcc caatcatttt acaagtagct cttggtcaaa
      661 gaaaagcctt ggctgtttat ggagacgatt atgatacacc agatggaaca tgtatcagag
      721 attatgttca agtagaagat ttgattgctg cacatatttt agctttggaa tacttgaaag
      781 aaggaaatga aagcaacttc ttcaatttag gcagcagcaa aggatactca gtcaaggaaa
      841 tgctagaagc agctagagaa gtgacaggaa aagaaattcc agcagaaata gcaccaagaa
      901 gagctggaga tcctagccga ttagtcgctt caagtgaaaa agcgagagag gttctgggtt
      961 ggaaacctga atacacagat atcaaagcaa ttatcaaaac agcatgggat tggcatgtca
     1021 gccatccaaa tggttatgaa gaatagggga atgagatatg tcaatcagtc aaacgatttg
     1081 tgattttgcc acacttgcga tcgaagctgg cggatggatg gaaatggacc gcttatattt
     1141 acagaatcgt cttttaggga tggtaggaga agatgctcta gaaagcattg aagtaagacc
     1201 agttaaggaa gaatctgtag acttattgga tcagcttgtc gctaaggcaa gagaaaatgg
     1261 tgttctttca gaattgccag cagaacaaga gatttttgaa gcgcaattaa tggattttct
     1321 cacaccgcca ccttcagtcg tcaatgcatt ctttgcacag cactattcga aaagcccaga
     1381 ggaagcaact gagtatttct ttaatttaag taagaaaaat gactacatca aaacaagagc
     1441 catcgcaaaa aacatcaaat ttccggcaga gtcggagtat ggtgaactgg aaatcacaat
     1501 caatttatct aagccagaaa aagatccaaa acaaatcgca gcagaacgcg aggcggtaaa
     1561 agtagactat cctacctgca tgctttgcat ggaaaacgaa ggatatagag gtcgcttgaa
     1621 ctatccagcc cgcaccaatc accggattat tcgaatgaat ctagacggtg aaagctgggg
     1681 attccaatat tctccttatg cttattacaa tgaacattcg atcattttat cagaacaaca
     1741 ccgcccgatg aaaatcacaa aggaaacatt tgctagactg cttcggatta ctgaagtctt
     1801 gcctcattac tttgttggtt cgaatgctga cttgccgatt gttggaggat cgattctttc
     1861 tcatgatcat tatcaggcag gacgtcatga atttcctatg gcaaaagcac cgatagaaaa
     1921 acttatcaaa ctagcagatt atccgcaagt catcgcagga gtcgtgaaat ggccgatgtc
     1981 tgtgatccgt ttgcaatcaa cagagaaaaa attgttgatt gaagcagcta actcagtatt
     2041 ggaaaagtgg aaagcatatt cggatgaaac agtatctatc agagcttttt cagcagatgg
     2101 tacaccacac catacgatta cgccaattgc acgaagaagg gacaattact ttgagttgga
     2161 ccttgtcctt cgagacaaca atgtttcaga agaacatcct gatgggattt ttcatccaca
     2221 taaagatgtc caacatatca aaaaggagaa cattgggttg atcgaagtaa tggggcttgc
     2281 tgttttacca cctcgtttga aaccagaact agtagaagta gagcatttct tattaggaga
     2341 agaaaaccag atggctgaat atcatcgtcc gtgggcagaa caattaaaaa aagaacatcc
     2401 tgatttgacg aaagatacgg tcaccgatgt cgtacaaaaa tctgtaggtc aggtttttgc
     2461 acgtgtactg gaagacgctg gagtattcaa gcgagatgaa aaaggacagg cagcgtttga
     2521 acgatttact gcaattttat aaacgtaaaa aaggaggaga gacaaatggc aacaattaaa
     2581 gacattgcgc agttggcagg agtttctcct gctactgttt caagagttct caactatgat
     2641 cctgatcttt ccgttggctt agccacaaag caaaaaattt ttgaagcagc agaacgctta
     2701 aattatacga aacatcgtaa aaatgcagca aatactaagc aaacgcttcg cctgatccag
     2761 tggtacgacg atacggaaga gcttgaagat ctttattatt tatctattcg tctaggaatc
     2821 gaaaaaaagg cagaagaagc aaacgttact ttattgaaag aaacgtggga ttccattagt
     2881 gatcttccga cagatggaac gatcgcacta ggaaaattcg acgaagctca gttattagaa
     2941 atgaaaggat cccaaaatgc cttgcttttg gtagattctg acggcacttc tcaaggaatc
     3001 gactctctag ttgtcgactt taagtcaagt gttcaaaaag tcattgaaca ttttgtctcc
     3061 cataaagcgc aacatatcgc tatgcttgct ggaacagaat atacgaagaa aaaccatcag
     3121 cggctaaaag atcccagaat tatggcattt caacaagttt tggcagaaaa aggctacgac
     3181 gaaggtccaa ttatcgaagc agatttttct gtcgagtcag gatatcaagc ggtgaagcag
     3241 tatttaaaaa cgaatccgca tgttcctgat gcgctttttg cagctaatga tgcattagcg
     3301 attggcgcac tgaaagcgat tcaagaaaaa ggtttagtgg taccagaaga tatttctgtg
     3361 atgggtttca atgatgtcag tgtagcgaaa tatgtaacgc cctctctttc tactgtaaaa
     3421 gtttataccg aatggatggg agagctagca gtggagacga ttcttcaatt aagtaattcc
     3481 aaagcaccgg tttcaagaaa gattattgtg gaaacagagt tgattattcg agaatcaacg
     3541 aaataaaaga tggaaaagct gtggggaaat gaagtgatac cgcagctttt ttgtgtttca
     3601 ggaagggaca aa
//
LOCUS       DAHHPA010000163         3597 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.171, whole genome shotgun sequence.
ACCESSION   DAHHPA010000163 DAHHPA010000000
VERSION     DAHHPA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.171"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            43..1236
                     /locus_tag="NBH55_002746"
     CDS             43..1236
                     /locus_tag="NBH55_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCD1510669.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            1229..2956
                     /locus_tag="NBH55_002747"
     CDS             1229..2956
                     /locus_tag="NBH55_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCD1510670.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            3350..3547
                     /locus_tag="NBH55_002748"
     CDS             3350..3547
                     /locus_tag="NBH55_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCD1510671.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT         1229 a          586 c          799 g          983 t
ORIGIN      
        1 ttcagaaaga aaaatgaatt tacataaaaa aggagaattt gtttggtaaa agtagaagta
       61 aaacatttaa caaagatatt tggtaaaaag acacaagccg ctttagatat gatgaatgat
      121 caccagccga aaacggagat tttaaagaaa accggtgcaa cggttggtgt gtatgacgtg
      181 aattttgacg taaaagaagg agaaatcttc gtgatcatgg ggctttccgg gagtgggaaa
      241 tccacattga tccgtctatt gaatcgtctg attgagccaa cttcgggcag tatctatatt
      301 gatggagaag atgtatcgaa gttaagcaaa gaagaattgc gagaagtccg tcgtcataaa
      361 atcaatatgg ttttccaaaa cttcggtctg tttccacatc gaacgatctt ggaaaataca
      421 gaatacgggt tagaagtccg tggtgtacct aaagaagaac gacaagaaaa agcagaaaaa
      481 gcattagaaa attctagttt gctttcattt aaagaccaat atcctagtca gttatctggt
      541 gggatgcaac aacgggttgg cctagccaga gctttagcaa atgatcctga gatcttgctg
      601 atggacgaag cgttctcggc tcttgatccg ctgatccggc gagaaatgca agacgaatta
      661 ctggacctgc aagccaatgt tcaaaaaacg attattttca ttacccatga tttgaatgaa
      721 gctttacgga ttggggacag aatcgccttg atgaaagacg gcgaaatcat gcagatcgga
      781 acaggtgaag aaatattgac caatccagca aatgattatg tacgtgaatt tgtggaagaa
      841 gtcgatcgtt ccaaagtgct tacggctcaa aacatcatgg tacctgcatt gacgacgaat
      901 atcgaatctg atggaccaaa cgttgcattg acaaggatgc gtaatgaaga agtcagcatg
      961 ctgatggctg ttgacagaaa gagacacctg aaagggatca tcactgcaga tcaagcgtta
     1021 gaagctagga agcaaaaacg tccattgatc gacttcctag atgaaaatgt gacggtgatt
     1081 gggaaagaca tggtggtaag tgatattttc aatattattt atgactcacc gactccactt
     1141 gcagttgtag aggatggaaa attaaaaggt gtcgtcatcc gaggtagtgt gattgaagca
     1201 ttagcagaaa caagcgaggt gagtgaacat gaataatcta ttgaactatc aattaccagt
     1261 ggcaaactgg gtagaaaata tcacagaatg gtttacgaca actttttctg gcctattttc
     1321 atttttacaa acgattggac aggcagtgat gtcggggatt acaaatttac tgttagtgat
     1381 tccagctcct ttgtttatct tgttattgac ggtagcagct ttctttattt ctaagaaacg
     1441 tccaggactt acgttgttca cattaatcgg tctatggttt atttataacc aaggtctatg
     1501 gaatgatttg atgaatactg tcacacttgt tcttttatct agtgtgatct cgatcatcat
     1561 tggggtacca ttagggatct tgatggcaaa aagcagtaaa gcccaaagta ttatcaaacc
     1621 gatccttgac tttatgcaga caatgccagg attcgtttat ttgattccag cagttgcatt
     1681 tttcggtatc gggatggttc caggggtatt tgcctcagtc atctttgctc tgccaccaac
     1741 cgttcgtttt acgaatttag ggattcgaca agttcctaaa gaattagtag aagcttctga
     1801 ttcattcggg agcacaagtc gtcaaaaact attcaaacta gaattgcctt tagctaaaag
     1861 tacgatcatg gcagggatca atcaaacaac aatgctgtca ctttcaatgg tcgttattgc
     1921 ctctatgatc ggtgcacccg gcttaggacg cggcgtatta tcagcattgc aaagagcaca
     1981 agtcgggaat ggatttgtca acggggttgc attagttatc ttagcaatca tcattgaccg
     2041 ttttacacag catttgaacc agccaaacaa taaaaaagct gcaggagcaa caacacagaa
     2101 aagtaaaaaa cgtcaaggac tgatcattgg tgctgtagta gtagtgatct taggtgcgat
     2161 tggtataggg agtttttcat ctgccaaaga aacaaaacgt atcaacttat cttatgttga
     2221 atgggataca gaagttgctt caacaaatgt agtcggtgaa gtattgaaac aaatgggcta
     2281 tgatgtaacg atgacgccat tagataattc aatcatgtgg aaatctgttt caaatggcga
     2341 gtcagatgcc atggtttcag cttggctacc taaaactcat ggttcacaat atgcacaata
     2401 taaagatcaa gtcgaagacc taggtgccaa tttgacagga gcaaaagttg gtttagcagt
     2461 gccagcctac atggatgtaa attcgatcga tgagttaacc gatcaggcag gtaagaaaat
     2521 cataggaatc gagccaggag ctggtgtagt tacggctgct gaaaacacga tccaaaaata
     2581 tgacaacttg aaagactgga aagtagagac atcttcttca ggagcaatga ccgttgcact
     2641 aggtcaagcg atcaaaaaac acgaaccaat tgtagttaca ggatggacac ctcattggat
     2701 gtttgccaaa tatgatctga aatacttaga agatcctgaa aacggaatgg gaagcgaaga
     2761 gcagatccat acgatggttc gaaaaggatt gaaagaagac cagccagaag cttacaaagt
     2821 attagacaat ttccattggt cagaaaaaga tatggaaaaa gttatgttgg aaatcaacaa
     2881 tggaaaagat ccacagcaag cagcaaaaga ctggatcaaa gagaaccaag aattagtcga
     2941 aagctggaaa aaataaaaag acagacacga aataaatgga gcttccaaaa ttggatcaaa
     3001 tatccaaatt tggaagctcc atgttcgtct ctatcttttt tgtctataaa aaaagagaat
     3061 gcgtagctca tcttcgaaac gttcaaagac gccgacaaac tattatgaat ttatgggtgc
     3121 gagtcaaaga gatcgatcgt aggtatgaaa gactaatgtg attaaagttt attatgtacg
     3181 aagtgcattc tcaaaaaata ttaaagaata tacatagttt atatacttaa aagtgattaa
     3241 attcttattc aggaattatt ttgctacgtt tcaagaagga attgtttttt gaaatttaat
     3301 gaatgttaga ataatagtct aaaaacatta atttaggggt gattagatta tgaaacattt
     3361 aaaaattttg tctattaaag agacacaact tatctatggg ggtaccactc atagtggaaa
     3421 atattatgga aatggagtgt attgcactaa aaataaatgt acggtcgatt gggccaaggc
     3481 aactacttgt attgcaggaa tgtctatagg tggtttttta ggtggagcaa ttccagggaa
     3541 gtgctaaaat gaaaaaaaat gctaagcaaa ttgttcatga attatataat gatatat
//
LOCUS       DAHHPA010000164         3279 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.172, whole genome shotgun sequence.
ACCESSION   DAHHPA010000164 DAHHPA010000000
VERSION     DAHHPA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.172"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(635..1591)
                     /locus_tag="NBH55_002749"
     CDS             complement(635..1591)
                     /locus_tag="NBH55_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510672.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1700..1945)
                     /locus_tag="NBH55_002750"
                     /pseudo
     CDS             complement(1700..1945)
                     /locus_tag="NBH55_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2090..2512)
                     /locus_tag="NBH55_002751"
     CDS             complement(2090..2512)
                     /locus_tag="NBH55_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510673.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2692..3063)
                     /locus_tag="NBH55_002752"
     CDS             complement(2692..3063)
                     /locus_tag="NBH55_002752"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCD1510674.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT          999 a          710 c          536 g         1034 t
ORIGIN      
        1 aaagaaaact tataatcaaa taatatcaac atttgatcac ataatttgag attgaatgct
       61 gatttaccta tgttttacgt aatggtcttt agttattttt gctcataaaa tgcataaaac
      121 acgcgttttt atgtgtgctt gaaatttctt ggctctataa tttctaagac tgataaaaat
      181 attctatcgg ttctttttgc aaataaaaaa acatatagaa gctccattag attgaatcac
      241 cacaacacaa tcaaaggagc attccgtatg cttgattttc attctataaa actactttta
      301 ggaatcaaag aaaatcacaa cggttcaaac taaaagcaag aatatccgaa gtaccattgt
      361 ttcatcgatc atgagaaaag cattcacctc tgtctgcaga tgttcttata tcgctctgtc
      421 agtcctatta ataaagtact gttttgtttt caaaaaaact attacgagta ctttttaaga
      481 gaatgccttg caaatattca tcacacaccg ttatataagt caaatagttt ctttcagtcg
      541 tcaagaaaag attctcctgt ccttggcatt atcagttaag tagcaactta attctcggac
      601 ttgttctact ctagcaaaat ttattttcat caacttacgt ccaaatgttt ttttgaataa
      661 ttacttcatc gcgggtctgg aagacttgcc tcattcgata agtcactagc ttttggcgtc
      721 cttcaggatt tctagtatac accaactgac atttgggcag cgacgattga tttaattcgt
      781 ttagaaaaag gttagcgatt tgcttatttc ggccaaagcg ttctggtacc gtcagaacta
      841 tctttttccc ttcgattaaa taccgcggat tctccagcgg tgctaaaagc tccctcatag
      901 cttcagtaaa aagctgtcct tctcgatagc cagcattctc aagatagcat ctaaattcct
      961 gctgatcagc cgctacgatt acttttctat cactaacttc accaaggcgc tgcaacgtcg
     1021 tttgaacgca tatggccaag ctttttttgc gggcacattc cttttcccat agcttttgtc
     1081 gcctacgaat ttggaaatat tccagcaagt tacctttaaa tatagctgac cagaagctta
     1141 gaggctctat attttttact actatcggca atgaagtttc ttgtttttgc tttacctgat
     1201 cgactttcct aacgacgatt ggcatagata ggtgccagcc atcagctaaa gcagtactcc
     1261 attgttcttt caaccgactt cgttcacaag aaaaatagaa catcgactga gtataagttt
     1321 ccatcgtggt ggtcgtatat tccagtggta aagcaaagcg atcgccacca ctctcaattt
     1381 ttggtgaaga ttcaaaggtc agacctgaaa tattgcccaa acgccgtagc attcttttca
     1441 aatcaggctg ttcagacaga ggaactcccg gtaatccaat cgctaaatgc caaatgttcg
     1501 ttactttatt ttgtgcacct aggcgtaagc cccgccctcg tagttgttgt gtttgcacaa
     1561 aagtggaagc ttgattagcc aagatcaaca gattaattgc aggacaatcc cagccttctc
     1621 ctagcaaact aacggttcct accaaaatat tcaacttctt ttcggttaat aggcgagtaa
     1681 ctgcttgcat caatgcttgg cggtttttgt catccaccgt caaataaaaa taacccgaca
     1741 tataaggatg ttcattcata cccgtcgcta gttctgccag ctcaacagtt actagcattt
     1801 tcggtaacag taagaactct ccacagatgg caccgatttg ctgagtgtta cctaatagac
     1861 gaacaacttc atcaaacaaa ggaaccacgc cataagctaa ggtaccatca ctgcctgcta
     1921 atacctcctt gcaaatacga tccatcaaaa tgaccgctgt cagttcttgc ttcagctgaa
     1981 cttcttcttt taacaaaatt tgacaaactg cttgcttttt ctcaggaatc gcctgactac
     2041 caacagtgtc tggatactct ggaaacaaat gcagcgactc atcaaacaat catccagctt
     2101 ttttcaattg ataaaaaata ggcaattccg gttgatcttc aaacatctgc tgataaatag
     2161 tgatcagctc ttttttacct aaagatggaa ctgaagcttt tttaccttgc tgcttagaac
     2221 tgcctaatag ctgccaatgt tcgggtatca attcatagtt atgatgattc aagtaaatta
     2281 atgcactagt gaacagctca aaattttcat aaatttcctt cgaatgctca agcggatgct
     2341 gaacaaattc taaagaaagc aaataatcca ccgtctcttg gctttccatt aacgcttcca
     2401 ccagctcatt ttgtttctct aaaaaggtaa aataatgctg agtagtttcc tgattagccg
     2461 gaatcaaata tagaaaatcc tgatgaggac atagaacctc ctcctttacc aactcgggaa
     2521 tgctaatttc ttcgtcaatc aggccatttt aactcaatat attgcttcca ctccgtctga
     2581 gagctatcaa tcggaggcgt ggctgtcaag gaaatcgtca acagattcgg ttgttttttc
     2641 ttcaagtcaa tcaacaattc accccagctt ttttttaaat gatgagcttc atcaaaaaac
     2701 taacagctcc agctgatcac cataagttgg ctcctccttc agtcggttgt aaatggtctg
     2761 atacgtttcc actgtaatat gactaggatg ttcaatcgcc ccagaaagat agcaactatc
     2821 tgctggattt actaagaaat attcctcgat tgttgttaac cactgtttct tcaatattag
     2881 ggaaggaacc aaaattaagg ttttacaatc caaacgtcgg atcatctcaa ttcctaaaaa
     2941 ggtcttgcca gcccctggtg gagccactag atggactgaa ttgttattta aataagtagc
     3001 aaagttagtc aatatacgct tctgatagct acgccactga attttttcat cagatagcca
     3061 catcccaggt ccccctctca cgtattttct ctatcataat ctaaatgaat acagcaaaaa
     3121 aaagatttct tcaagaatat gtgtttttat agtaatattt tacttactct aaactcaaat
     3181 gaaaaggtaa aaaaactggt agtacaatct tttaaattct cattgttttg tacgttcttt
     3241 actatcaaaa aaacaaaaaa aacgcgtttc tacgcgtga
//
LOCUS       DAHHPA010000165         3264 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.173, whole genome shotgun sequence.
ACCESSION   DAHHPA010000165 DAHHPA010000000
VERSION     DAHHPA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.173"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..218)
                     /locus_tag="NBH55_002753"
     CDS             complement(<1..218)
                     /locus_tag="NBH55_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCD1510675.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGL"
     gene            296..604
                     /locus_tag="NBH55_002754"
     CDS             296..604
                     /locus_tag="NBH55_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510676.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(575..832)
                     /locus_tag="NBH55_002755"
     CDS             complement(575..832)
                     /locus_tag="NBH55_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCD1510677.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1130..1270)
                     /locus_tag="NBH55_002756"
     CDS             complement(1130..1270)
                     /locus_tag="NBH55_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510678.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            1564..1884
                     /locus_tag="NBH55_002757"
     CDS             1564..1884
                     /locus_tag="NBH55_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1510679.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1902..2333
                     /locus_tag="NBH55_002758"
     CDS             1902..2333
                     /locus_tag="NBH55_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCD1510680.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            2460..3044
                     /locus_tag="NBH55_002759"
     CDS             2460..3044
                     /locus_tag="NBH55_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCD1510681.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            3158..>3264
                     /locus_tag="NBH55_002760"
     CDS             3158..>3264
                     /locus_tag="NBH55_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HCD1510682.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKG"
BASE COUNT         1112 a          525 c          565 g         1062 t
ORIGIN      
        1 aggcctgact cgttaatcaa aacaactgct gttccattga cggtgaacga atcgttctgc
       61 gtcttatcct ccaagtccac atgatctcga atagcttttt gcggattcga atatcctaga
      121 acgtctgcta catcttttcc tacaaaatat ggttcatcat ttactaaaat agttcgaact
      181 tcgttttgct cgaaattaaa aatttgtggt gtattcatat ttctcattcc tttctttggt
      241 ataatttcct tatcagtgtg acaggctgaa ataattgtta aggaggtgaa aatttatgaa
      301 accagaattt gtaattacta tgcttgatgg agaaaaaatc aaaatatttg ccgatacttt
      361 ggtcgttgga tacgatgatg cgtcaaatat cgaagaaaaa gatggaatgt tctattcttc
      421 tcaaatttac gtgaatacct tgcaaggtga tattggggta tctactcttg caacatcaag
      481 accgcttgta ggtcttatcg gctttttgtc aaaagcaaca ttcttttctc tcggcgatga
      541 tatagattcg gatgaaactg tcatctataa aacatcagct gttaaaagta tcggtttcaa
      601 atagtaattc ttgctcgttg atagcggaag ccaacttttc ttccaacaca tcggcgagct
      661 ttctttttgt tggattcatt tcaaagttga tagtcttgag aagcattttt gcatccttaa
      721 ctttttgtcc ttgcaattcc ttgatgattt tatcggcttg gctatcaatt ccatgtgtta
      781 gatcatctat cattttttgt accttgattg tttggctcat ttccttgttc attgtttagc
      841 ctcctttaat aaatgatttt ttgtattttt taatttccat aaattcctca aattgctctt
      901 tggtattatt accacgacca tacatattgt gaaattcttt atggcaactg acacaaagtg
      961 taacgccgtt tgaaatcgct gcggatttag ttgttgcatg gctaaatcac gaatcacaag
     1021 tatctactaa aggcgaatca atttcgccaa aagaaattgt tcaagcttat ttagatattc
     1081 attatgctgt tatctatggt cagcttccag aagaccgtaa aaacgatgac taaacgtgag
     1141 ctaaaatctc tgctatggct gcaaccatgg cagagtcttc tttatctacc gcgtgttcca
     1201 tcgccacttt tgcttgtttc agtatgtcta atttcaaatc ttcgattttt gccgagactg
     1261 ttttttccat attcttctct cctttctttt atattcgtaa acaaatttaa caacttttta
     1321 aaaaactgcg ttgactaaat taacatacag tgttaaaatc agaacatagt taaataagac
     1381 atataaacat tgattttaaa agctttcttg gcggttggca tttattaatc aatagtgttt
     1441 tttgttgtct ttttagttgt taaacttgtt tacaagaatg atattaacac cttttgttaa
     1501 aatagtcaac gaattttaac aaataaagtt aaatattttt tcttgaacga aaggaatatt
     1561 gatatgataa cgtttgagag aataaaagag ttagcgaaaa aacagggaaa atcattaaat
     1621 aaagttgaag aagacctagg ctatggtaaa aacgtcttat atagacttaa gaactcaaac
     1681 ccctctacag aacgtttgca agaaattgct gactatttcg atgtctctgt agactatctt
     1741 ctggggcgcg aagaaaaaga aacccctaaa catgtggatt tatcagaaaa cgatactgtt
     1801 ttttcttttg atggaaaaga aatatctaag gagacaatgc gtaaagcgat tgcaattgct
     1861 aaagctttag aggaaaatga atagttggag tgatgggttg tatgtatttg aaattgaaag
     1921 aaatgttgag cgaatacaat ttgaaagtag tttacatgga aatgaaagag cctggcttct
     1981 attatccgaa gcctagaatc atttttttga atgaaaatct atatggcgaa actgcagaag
     2041 cctttcattt atctcatgaa cttgcacatt tcagcgcctc tcattttgaa ttttcagttt
     2101 tgtacgatac ttctacaact tttcactcaa aatttgaaac agaagcagat aaaatagcta
     2161 ttctaatttt attaaatatc tatattgaga acgaactgac tgatgaatct cagtttaatc
     2221 tcgaaaaatt tatggagtat tattctattc agaataagct cagatacact tgttatgctg
     2281 tctgccaatg ctattttaag aaaaaatatt cttacgcaag gcaatatgta tgaatacatc
     2341 aagaattatt aaaaaaaccg atttcagcaa aacattaaaa aagcccgtgc tggcacacgg
     2401 actcatacct catttctgag atcgcaaata tattataaca agaagtgagg aatatttaaa
     2461 tggcaaaaaa agttatgggt caagacggaa aaatgtataa agttagtaaa cctttttaca
     2521 aaaaagtatg gttttgggta ttagcagtaa ttttaattat aattattggt tctgctttaa
     2581 atggtggatc agatagtaat aaagcaagtg ataatggtgg cgaaaaagta actaaatctt
     2641 caacctctgc ttcatcttct aaagaagaaa aaagcgatac cttctataag attggtgaca
     2701 ctgtaaaagt tggtgatgct gaatatacac taaatagtgt agaactaact gatgaaagaa
     2761 atcaatttga agaaaaccaa cctgcacaag tagtaaaaat tacttatacc gtaaaaaatg
     2821 atggcgattc agatatccct gtaggtacag atgtggaagt atatggacca gatgacaaaa
     2881 aatcagagac atatgccaac gaaaatacaa tgggatctgt tgctccagga aaacaaatgg
     2941 atgtaactgc tcatttcacg ttaaaccaaa agggagaaat agaaatccac ttctctcctt
     3001 tagtatcatt tgaaaaagca gctattttta aagcaactgt ataataaata aaaaacacgc
     3061 ccaccgtcca aagcaagcgt gttctaagaa aaacaaacct atacagtagg cttctttata
     3121 gtgcctattg tatcagagaa agagagtaga ttcaattatg gcaagattag tcaaacgtgg
     3181 aaatagttgg caatacgaaa tttcatacaa aaaagatgac ggaaaataca cgaagataag
     3241 aaaatcagga tttaagacaa aagg
//
LOCUS       DAHHPA010000166         3155 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.174, whole genome shotgun sequence.
ACCESSION   DAHHPA010000166 DAHHPA010000000
VERSION     DAHHPA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.174"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            551..883
                     /locus_tag="NBH55_002761"
     CDS             551..883
                     /locus_tag="NBH55_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCD1510683.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1502..1843
                     /locus_tag="NBH55_002762"
     CDS             1502..1843
                     /locus_tag="NBH55_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCD1510684.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(2080..>3155)
                     /locus_tag="NBH55_002763"
     CDS             complement(2080..>3155)
                     /locus_tag="NBH55_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCD1510685.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1012 a          475 c          652 g         1016 t
ORIGIN      
        1 aaacaagcta aaaatcttga tattacttct tttcaggcaa tacgaatatt attcgcttac
       61 attttttgta taaatttaat tgtctgctat gcttgtgatg aattgatttt cgttctgcac
      121 ttttggatga taaatttgga caattgaaaa tacaaaaaaa agaaaggcgg tatgaactcg
      181 gaaggctggt aaccttcgtc agcgttcata cacggcatta agatttggcc cttaatgcgt
      241 taacctttca gtgttacggt attacccgca atggatttag tataacagat attcaaaagt
      301 gataccactt tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg
      361 cctaagcaca atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa
      421 ttagagggaa cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga acgaaaatca
      481 attctctatt tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gaaattgggt
      541 ggtttatttt atgtcatata caggaatttt gagtctcaaa gatatttgtc actacggtaa
      601 acggtgtacc gctacagaga aaatcacgaa gaaactatca actggccaaa gtaaaaccgt
      661 tgtgcagtgt aagaaatata taatccagaa agacaaagtc tcagaggaaa tgatttatta
      721 cattgggaaa caaaagcaaa taattttaaa agatcctatc cccttaaaag agctatatcc
      781 aacaatcaaa catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt
      841 cttgcgttgt accgcaaaag ggatggggcg tttgatcggc tgaattgatc attctcataa
      901 cttgtatttt atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta
      961 gagtcactag aaaaataagg agtagacttg atctatcaac aattcccttc ggggagtgac
     1021 ggggtaggtt ttaacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcgcttgtt
     1081 tggatagtgg tgaaatgaat ttgtgtaaat aataagcgca gagtaaacaa gagggaattt
     1141 agaagttaga caagttagaa tagcgtctag attaatcatg agcagctact aagggatcta
     1201 gactttgacg caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca
     1261 agaaattgtg tcgtgtgctg cagtacatgt agatacttgc gaggacgttg attattggat
     1321 caatcgagca tttaccgctg tctactaggg tttctcgttt cggacgccat aaacgagtcg
     1381 aaatttttta tgtattttta agtaaacgaa aaacgataaa atatgaacaa gtcctgaata
     1441 aagtgaaaaa attgatataa aacatagaga aaatgaaatt tacgtaatgg aggaatgaca
     1501 aatgaaaaat tatgatccga atatacgatt gggcacacat actattaagg tttctttcca
     1561 acgttgggat tacaaaggat ttgtgacgtt tagaagaggc ggaaattgta aaggattaga
     1621 tgttttagca ttagacgagg atgatttata cgatcaaaca ttgactgata atccaattgg
     1681 attcggcttg ctacctgaag atgatgaagg taatgaatgg tttaaaatga cgcttatgaa
     1741 tgataatgga gacgaattat ctgttgaaga tacttggtca tatttgagtg attatattgt
     1801 cagctttgaa atcattgaat ttgtagcgga taaagaagaa taaaaactgg aggaataaat
     1861 tttacagata aaggcttaga aggagtcacg atgattaata aaaaggtagt attaccttag
     1921 gcattgagta aggaatttat ttagtctaat ggtgataagt caagatgaaa tttaagattt
     1981 tctttattga gtaatatgtt ttgaagcgca cgaaactaga cccgatcaaa atcaaatttt
     2041 tttcgggttg attcaaggat ttgcgtcaat aacggattgt tatttctcta tcacactcct
     2101 tggtggcgta tagagttggt gtttacgtag tagcgtatcc accagacgcg taaattttct
     2161 tgcggttagg acgagtgcac gtttgtgttg atgctttgga acctccttat atttactttg
     2221 ataaaaagct ttgtattcag gaagataatt ttttacagag ttggcagctt caactaagta
     2281 gtatctaaaa tatcgatttc cttgttttgt cagaggtgta ttttgcgact gatagtttcc
     2341 agattggttc actttccaac ttaatccagc gtattttgcc aattttgtct gatcttcaaa
     2401 tctttcgatt tgaccaattt cagcgattaa accggcagca tagactttac caacgcctgg
     2461 aatactggtt aagcattgat attctggaat gacaacaatt acttgttcga tggctttatc
     2521 taattctttg atattttttt caagtgctcg tatttctcgt acgagaacac ttaaaacaac
     2581 attaattgat tcttgtgcaa gagcacctag gcggtaactc atggtaattg cacgttgaat
     2641 tgctttagcg attttttctg gttgcttgaa acggcctttt cctttttctt gaagtaaatc
     2701 acagaaagct tctaaaggaa gctcagcaag ttcatccaat gtgaaatctt ccgtcatgag
     2761 tgaaatgagg gttgcactaa aaagactcgt cgattcgtct cgcatttctc gagcaagcgt
     2821 attacatttg tacgttagat tttcaagaaa atgttgcttt gtacgaatca attgcttaat
     2881 aagttgatag cgagtacgtg ttaaacgctg taaggccatg tatttctctt ctttgatagg
     2941 ggaagttgtg aatcgttgaa tacgcaagaa atcagcgatt cttaatgcat cgtttcgatc
     3001 agttttattc tcatcaaaca tgcgactaaa ttgtttcacc cgaaaaggat tttcaatcgt
     3061 tacaagtgtg ttgagcttct ttagtttctc atcttcatga aaaaacatgg aaggatggaa
     3121 gctgtacatg gacgttgact ccatgccaat cacaa
//
LOCUS       DAHHPA010000167         3103 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.175, whole genome shotgun sequence.
ACCESSION   DAHHPA010000167 DAHHPA010000000
VERSION     DAHHPA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.175"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            87..569
                     /locus_tag="NBH55_002764"
     CDS             87..569
                     /locus_tag="NBH55_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510686.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            766..1656
                     /locus_tag="NBH55_002765"
     CDS             766..1656
                     /locus_tag="NBH55_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCD1510687.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(1943..>3103)
                     /locus_tag="NBH55_002766"
     CDS             complement(1943..>3103)
                     /locus_tag="NBH55_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCD1510688.1"
                     /translation="KLDDSSSYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQPRMNFLPTVLCMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYK
                     LTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQL
                     RVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVD
                     ELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQS
                     GISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          968 a          555 c          651 g          929 t
ORIGIN      
        1 tctaaaaaaa aagtttttcc ccctaatttt tttagataga aaattgtata gtatgatgag
       61 agaaaaagta gtttggggga acgttaatga gaagaaaagc agtaatcgat cggctttatt
      121 tatttttttg gttttttcct atcgttttgc taccttttac atgggtaaat gctgttgtac
      181 aaggtgcgga gatattaggc ctgatccaag gaagcggatt tgtggtttct aggaattttc
      241 cagctttgat tgttttctat ttagtgataa gtgtaggtct cttattacca aaatctctga
      301 tcaatcacta caattggcag ctgctttact tttcttgctt attagtattt acatctatgt
      361 tttctttgct gcctcgcttg attatcggcc ctaatggttt gagcgagagt ggattgaagg
      421 atttagctta ttcctccatg tatttcttga cgatcaaacc atggttctat ttagtaattg
      481 cctcatttat tcttgcaaca tttttttact tctggacaga aatcaggata aaaagtgaac
      541 gatctgtatc tataaaaaat aactattgat ggtggatcat cagtaaaaaa acatatgagc
      601 tgcgtaaaat gcatgtttct catatgcttt ttttattatt atgtaaagaa tgcgaaaaac
      661 gtttacatga tttttacata acttaactat atttttacag caaccatacg agggatttac
      721 agtcgactga taaactttag ttgtaaataa gaaaggagca ataaaatgaa aaaagtattg
      781 tttactctag gtatgatggg aattctttta gccggatgcg gctcaggatc aggaaataca
      841 gatggctctg ccacacaaaa taattcaagt gactcaaatg aacaagtaaa aatcgttgcg
      901 gtaggttcga cagcattaca gccgttagtc gatgcagcac aagaaagctt tgtacaagaa
      961 aatccaaatt atcaaatctc tgtacaagga ggaggaagcg gaactggttt gagccaagtc
     1021 gaagctggag cagttactat cggaaactct gatgtatttg cagaagaaag agacggagtt
     1081 gatgcttcaa aattagtgga tcataaagta gcagtggtcg ggatggcacc aatcgtcaat
     1141 aaagatacag atgtcaaaga tatcaccaaa caggaattga ttgatatctt tacaggaaaa
     1201 atcactaact ggaaggaagt tggcgggaaa gatcaaaaaa tcaacgtcgt gaaccgtgca
     1261 aacggcagcg gtacccgcgc gacattcgaa aaatggggac tggatggtgc tacccctgtg
     1321 cagtcccaag aacaagattc atcaggaaca gttcggcagc tggtaagtca aacaccagga
     1381 gcaatcagct atttagcttt ctcatatctc gatgattcca cacaagcact aagtatcgac
     1441 ggtgtagaac caaaagaaga gaatgtagca gataacagct ggggaatctg gtcatatgaa
     1501 catatgtaca cgaacggaaa accttcccct gaagtccaaa aattcttaga ctacatgatg
     1561 acagaagaaa tccaagaagg acctgtcaaa gaattgggct atttaccaat cacaatgatg
     1621 gaagtagaac gtgatcacga aggaaatatc aaataactag cagataaaca tgccacggta
     1681 gtcgttacta ttaaggaaaa atcaactatg tttccccaca tatacatttt tcctttaatt
     1741 gtgaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt
     1801 gaacaccgtt tattcgaaaa aaagagctac gacatagaac tgtcgtaact ttttttggct
     1861 cttcgtcaaa tcgtgttggt gaagacaaat ttgaataaaa cttagtccga tatgcggcgc
     1921 gttctgtgcg ccgcatattt ttttagtttt caaagatcag accttgttgt aaatagatcc
     1981 gtgcccgaaa gttataaaag ttgcgataac caaacgcaat tcgtttaatc agcttgatct
     2041 tgttattcag cccctcagtg acgccgtttg agtgggacac cttaaaggca tttgaaatac
     2101 ccgattggta gcaatggaag atcgtgaatt tctttttaaa cgactgaggt aacgtagatg
     2161 aaattgtttt aagggtttct tgaaagcctt ccgtacctcg tttaacgaaa taatagcgta
     2221 ataattgaca aaaatgatac gcagttcgca attcttcgtt gtagcttaac agctcatcaa
     2281 cgatttctgt ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt ttgagtggct
     2341 cccatgtcgt ggaatctttg agtaagagtt tccaatatcg cttgagccga cggtacttct
     2401 tctgattttc agagtgtgag gttcgaaaac gattcatgat ctggacacgt aattgattga
     2461 agctgcgatt gatgtgttgg acaatatgaa agcggtcagt tacgatctcc gcatttggaa
     2521 agactgtttt aagcaactga caatagctgg cattcatgtc catcactagg tacttgacct
     2581 ttaaacgggc ctttctagga aagcgcagga agtattgagt tagtttgtaa agtctgcgat
     2641 cttcaaggat cgtaaatagc tgattggttg tcccatcaac acaaataaaa ctcatggaac
     2701 cagaaacaga attcattgat ttgaactcat ccatacacaa caccgtcgga aggaagttca
     2761 tacgtggctg gtaatgcttc gttgcttcac gcaagatccg tgtcaccgtc ccatccgaga
     2821 cgaagtactt cttagcgatt agtttacgtg attgattttc gtacagttcc atgagaatcg
     2881 cctgcttcag ttcctttgtt agttgatgat gttcatcaac taagtccgtt ttcgcagaaa
     2941 aggtccttcc acaatcatgg cagagaaaac gggtacgttt taattctaaa atagttaact
     3001 tttctttgac tttcaacatc tgagtgcgtg tcgtatagga accgttgcgg ataatacgaa
     3061 ccgatcgaca gtgttcacaa taggaagaag agtcatccaa ttt
//
LOCUS       DAHHPA010000168         3043 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.176, whole genome shotgun sequence.
ACCESSION   DAHHPA010000168 DAHHPA010000000
VERSION     DAHHPA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.176"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            219..656
                     /locus_tag="NBH55_002767"
                     /pseudo
     CDS             219..656
                     /locus_tag="NBH55_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(733..2280)
                     /locus_tag="NBH55_002768"
     CDS             complement(733..2280)
                     /locus_tag="NBH55_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCD1510689.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(2382..2735)
                     /gene="tnpB"
                     /locus_tag="NBH55_002769"
     CDS             complement(2382..2735)
                     /gene="tnpB"
                     /locus_tag="NBH55_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCD1510690.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2725..2919)
                     /locus_tag="NBH55_002770"
     CDS             complement(2725..2919)
                     /locus_tag="NBH55_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510691.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          904 a          616 c          547 g          976 t
ORIGIN      
        1 ttttaatgtc ataatcgttt aaattttctt gattgacatt tatttacaaa aaatattgcc
       61 atttaaaaag aaaagtgaca catactattt cctccatcat tagtttttgt ctatataagg
      121 tataccctaa ctagacacga ccacaacatt gaaaaatctg gttttatcgt gtatgattag
      181 ggtgtatttt aaatagttgc agaaagtagg taaaaatttt gagagttgca tacatacgtg
      241 tctcatccat cgatcaaaat gaacaacgcc agattgaaga aatgaaaaaa ttcggtgcag
      301 tgagaatttt catcgagaaa caatcgggcg caacgattac tcatcgacct gttttccaag
      361 aagcattgga tttcgtccga gatcaagaca tttttattgt ggaagctatt gatcgacttg
      421 gaagaaatta cgacgagatc atcgcttcag tcaattacct aaagaagaaa aacgtacagc
      481 taatcatcac cagtctaccg atcatggctg aagcaattgg caaccctctc ctggataaat
      541 tcattaagga tctgatcatc caaattttgg ccatgatcgc tgaacaagag cgtaccgaat
      601 ctaaacgacg ccaggcgcaa ggcattaaaa ttgcgaaagc aaatggagtt tataaagtaa
      661 gcgccccata gaacagtacc tgtacgtcaa ataacccgac ctatagaata gaatcgggaa
      721 tgtttggcgt ttttagttac gagaaaaatg ttgttgtaca tccttgttcc aaggcaacag
      781 ttgatctaaa gtttctgctg aaagtacggg aagatttggt atcttatcaa atagaaactc
      841 tagatacttt acaggattca atccgttaag ttctgctgtt ttatatacac tcagaatgat
      901 tcctgaagtt cgtgctcctt caagacttgt ggaatgcaac caatttttcc gtcctatcac
      961 cagctctttg atatttcgct ccgcacgatt atttgtgagt tctaaacgac catcttcaaa
     1021 aataggttca aaaccacttc gtagtttaca cgcatattga acggctgcat ccaacttgga
     1081 ttttgcaaca ggatctatcg ttgccatcca ctcataaaaa gcaataaata atggacgcag
     1141 ttcttgttgc cgacagctta atcgcttctc aggagaaaag tctttccatt tcttttctag
     1201 gacaaaccat ttatccaatt gtttgactgc ttgagctgcg ggaatatccg tgtttttctt
     1261 tccttttgga attgcttcga aaaatttccg ccgaacatgc gcgaaacaat agacaagtcg
     1321 tacactttcc acggcattat acccgctata gccgtcacag tgtaaatacc ctgtaaactc
     1381 cttgagaaaa gttttcggaa catctccagc acgactttcg tcgtggtgat aataaacgat
     1441 gggatgctga ctgtcttttc ctgtacaaaa ctgccagtaa taggtatctg ttttggcact
     1501 ttcaatcaca cgataacttg tttcatcccc gtgtagaatc tcttcttgat taagatagac
     1561 tcttaatcgt tctcctaaag ggtctaacgc gttttgcact gctttaatat gccaattggt
     1621 gatattatct ctcgaaatat ccaacccatg tgtttgtttc caatagttct cttgccgata
     1681 ggcaggaact ttctgaccaa atttcatccg gatcgtttcc gccactaaac tattggaacc
     1741 taagctatta gaaatcaaag gtttaggaac gatcgctttt ttaaaagaag aaagtccttc
     1801 tttttcgcag cttttacaac aataggtatg ttgataatgg gtgagacaat ccaacatagc
     1861 ctgatggaaa cgaatttctt ctcgtacttt tgtccgtccg atatctttca tctgtgaacc
     1921 gcaatgttca caaaattgtt cttccaaagg aaggagatgt tctttctctg ttataggtaa
     1981 gtgtttgatt ttgtctgctt tcgttcctac aactcgttga tgtcctttta ctggaataat
     2041 ggtggcagct gcttctggaa cacttacaga aatttcttca tcactaaaaa gattcaattg
     2101 cccattcgtt tcctcaaaga gagtctcttt agagcgtcca aataatttgt tggttaaata
     2161 attgaccttt tcattcaata gttggatttc agttgtaaga ttctgaatgg tttgttgttg
     2221 tgcttgattg gttttatggg cttcttgaag caactgaagc aacgtatctg tagtttccaa
     2281 aatttttcac caccttgaat cgaactgatt ttgttacaga ataccacaag aaacgcacaa
     2341 gaaatagggg ttcaacacat tttttgtgtt aaccccccag cttaaaaaac tccttttttt
     2401 cctggcaaaa tagctcgagg ttgatcgata cttaatcctt ctaacaacca tcgaacctgt
     2461 tggttcgtta aggttcgaac ttcgtctttc gtccgaggcc attttaactt gccgttttct
     2521 atccgcttat aacagagaag aaaaccatca ccgtcccaat agagaagttt gaaacggtct
     2581 tgacggttcc cgcaaaataa aaaaacggca tcttcgtaaa gctcgagttg atactcttgt
     2641 tgaataagac tggcaagacc atcaattcct tttcgcaaat ctgtttttcc gcacacaata
     2701 taaatatgtt tggtttgaag taaatcataa agcattggac aacagttctt tcaaaatagc
     2761 ttgaagaata taattgttaa taccgttata gatcaaaact tcagaaccat tctctctttt
     2821 gatgcgcatc gctaacttgg cagccgcggg tttacctttt tctggtgcac tcttttcgtt
     2881 tcctaattgg acaggaacaa tctcatttaa tttatacata gaaaacctcc agacttcttg
     2941 atagctcaag tatatcttga ggtgcatttg ttagtaaggt actgttctat ggggcgctta
     3001 ctttataaag gtcgtccaaa actctatagt gcagatacaa aaa
//
LOCUS       DAHHPA010000169         2945 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.177, whole genome shotgun sequence.
ACCESSION   DAHHPA010000169 DAHHPA010000000
VERSION     DAHHPA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.177"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="NBH55_002771"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="NBH55_002771"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCD1510692.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="NBH55_002772"
     CDS             complement(1061..1981)
                     /locus_tag="NBH55_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1510693.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="NBH55_002773"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="NBH55_002773"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCD1510694.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          823 a          601 c          509 g         1012 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaact
//
LOCUS       DAHHPA010000170         2890 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.178, whole genome shotgun sequence.
ACCESSION   DAHHPA010000170 DAHHPA010000000
VERSION     DAHHPA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.178"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..1087
                     /locus_tag="NBH55_002774"
     CDS             <1..1087
                     /locus_tag="NBH55_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1510695.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="NBH55_002775"
     CDS             complement(1305..2447)
                     /locus_tag="NBH55_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1510696.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYMLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTIEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="NBH55_002776"
     CDS             complement(2452..2637)
                     /locus_tag="NBH55_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCD1510697.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          959 a          528 c          453 g          950 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct gtgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caatggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacatata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat
//
LOCUS       DAHHPA010000171         2890 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.179, whole genome shotgun sequence.
ACCESSION   DAHHPA010000171 DAHHPA010000000
VERSION     DAHHPA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.179"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            132..1550
                     /locus_tag="NBH55_002777"
     CDS             132..1550
                     /locus_tag="NBH55_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1510698.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            1562..2872
                     /locus_tag="NBH55_002778"
     CDS             1562..2872
                     /locus_tag="NBH55_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCD1510699.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT          934 a          449 c          605 g          902 t
ORIGIN      
        1 gtttttagat tattttttgt tttttatctg tcaagataat gcaataaaaa gccaacattg
       61 tgaatataaa aacaagaaaa aaagagttat aattttgaaa acgtttacta gaagaggtag
      121 gaggaattca aatgctttca caaaatgctg ttacagcaaa tacaataaaa aaagaaaaaa
      181 aggaatttgg gatttccgat aagataggct acatgttcgg tgatctaggt aactgtttta
      241 ttttaggttt agtcaatagt ttcttgatga tttattatac gaatgttctt ggcgtttcag
      301 gggcaatcgt aggtacgtta ttcttgattt cgagaatttt ggatgcgttt gctgatgtga
      361 tggttggaag attatgtgat gtctcagagt taacagaaac tggccgattt attccttgga
      421 tcagaaaaat gaagtatcca ttttgcttag tgactgtcat tttgtttttg ccattcgtcc
      481 aacatcttcc aatgacggtg aagattgctt atatttttgt ttcatacatt gtttatggga
      541 tttttctctc aacaatcaat atcccgtatg gagcaatggc agcagcaatt agctcaaatc
      601 ctaatgaccg cacctcgttg tccacgttcc gaagcattgg ttctgcgatt ggcggatcaa
      661 ctacaggatt cttcattccg attttaatgt atacccataa caagagtgga caacaagtcg
      721 tttcagggaa tcatttcttt tggatttcaa tcggatgtgc tgcaatagca tttgtcgcat
      781 atatgttaac ttgtaaattg actactgaaa gagtaagagt tgtcaaaaac gaaaaagtat
      841 ctgcttctca gttggtaaaa ggtttgttag ctaatcgagc actgattatt ttagtcattg
      901 ttgacatttt cattgtgatc aatcagattt tagctgggac gaatatgact tatttattca
      961 atgattactt ccataataaa caagcaatgt ctgttgctct tttatttact tacggaacag
     1021 tagtagtttt agcaccattt gctacaaagc tgacaaaatt atttggtaaa aaggaagcaa
     1081 gcgtatttgc gttacttgct tcttcatgta tgtacttgct gatgtatttc cttcatataa
     1141 caaatgcatg ggtatatctg attcttttat ttatcgctac tttaggatct ggtttattca
     1201 atttgatggt ctgggcattc attactgacg tgattgatta tcatcaatat aagacaggct
     1261 taagagaaga tggaacagta tatggagtaa attcttttgc gagaaaagta ggacaagcat
     1321 gtgcaggagc ggtaggcgga tttatgctgg cactcattgg ttaccaatcg tctacaacag
     1381 gtggagccat ccaatcagtt ttagtacaag aaaggattta tacgatggca aacttgttac
     1441 cagcattctg cttatttgta tccgcaatca ttttgctagt aggttaccca ctgaatcgtt
     1501 caaaaactgt atttattggt gaagaattga aaaaaatcaa tgaaagatag ggagaaacgt
     1561 attgcggaca aaaaaaggga ttatcaatgc tttgaacttt aaaacaaacg acactcaaca
     1621 agatgataca ataatactgc aaaaagcaat cgatcaaggt gcgaaagaac acttacctgt
     1681 atttattcca aaaggcattt atttagtcgg agcccttttt ttaaaagata agagtcatct
     1741 tatctttgaa gaaggtgcag tcctcaaggg gagaacagat atcgaagcat ttccagaaat
     1801 cgatacacga gtagccggcg tagaaatgaa atggccagca gcgattttga atgttctctc
     1861 ggcgaaagat attcttatag aaggaaaagg aataatcgac ggtcaaggag accattggtg
     1921 ggagctgtat tggggaaaag atcaaaaaag cgggacaaga gctgaatacg atcaaaaggg
     1981 acttcgttgg attgcggatt atgctattaa acgtccacgc gcttgtttac tgtatcatgc
     2041 agaacatgtt gttgttcgtg atttgatttt tcagaaatca ggtttttgga acttgcaaat
     2101 cacttattct aacgatgtat tagtagaaaa agtaatcatc cgcaacaatg atggaccaag
     2161 tacagatggg attgatattg attcttcaac aaatgtgcgt gtctatgaat gtgatttggc
     2221 ctgtggtgat gattgtattg ctattaagtc tggcagagat ggcaatggtg caagagtcaa
     2281 tcgaaaatca tcaagaatcg aggttgctag atgtaaaatc cgttcaggat acggtgtaac
     2341 tattggtagc gaagttagtg ctggagttag tgacgtatat attcacgata tcgatttttt
     2401 tcagtcagac tgtggtattc gaatgaaatc ttcaaaagaa agagggggag tgatcgaaaa
     2461 tatcagggta gaaaatctga atatgattga tgtacaattt cctttttctt ggataatgga
     2521 ctggcataat gaatacaata gaaaaaatag caatgacctt gctcaaatgc cagaaagttg
     2581 gcaagcagtg gctgaagaaa tacctgaatc aaagcaaatg acgaaagtca aagacataca
     2641 catcaaaaac gtacaggcat gcctttctcc aggttactca cttccttccc gagcttttga
     2701 tttagtagca tttcctgaaa agcctataga agatgtctgc ttttctcatt gccaaataac
     2761 tgcaaaagaa ttcgggcgaa tcgaagcagt acaaaactta tgtttcaaaa gctgtgattt
     2821 atctatagaa actggcaaca cagctgcaaa caatactttt gacaatcgtt gaaattataa
     2881 taaggtcaag
//
LOCUS       DAHHPA010000172         2887 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.180, whole genome shotgun sequence.
ACCESSION   DAHHPA010000172 DAHHPA010000000
VERSION     DAHHPA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.180"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(150..512)
                     /locus_tag="NBH55_002779"
     CDS             complement(150..512)
                     /locus_tag="NBH55_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510700.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(586..966)
                     /locus_tag="NBH55_002780"
     CDS             complement(586..966)
                     /locus_tag="NBH55_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCD1510701.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1030..2223
                     /locus_tag="NBH55_002781"
     CDS             1030..2223
                     /locus_tag="NBH55_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCD1510702.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2300..2884)
                     /locus_tag="NBH55_002782"
     CDS             complement(2300..2884)
                     /locus_tag="NBH55_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD1510703.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          898 a          560 c          484 g          945 t
ORIGIN      
        1 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
       61 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      121 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      181 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      241 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      301 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      361 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      421 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      481 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      541 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      601 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      661 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      721 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      781 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
      841 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
      901 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
      961 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1021 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1081 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1141 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1201 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1261 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1321 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1381 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1441 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1501 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1561 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1621 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1681 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1741 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1801 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     1861 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     1921 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     1981 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2041 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2101 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2161 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2221 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2281 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2341 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2401 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2461 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2521 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2581 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2641 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2701 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2761 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     2821 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     2881 ccatatt
//
LOCUS       DAHHPA010000173         2870 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.181, whole genome shotgun sequence.
ACCESSION   DAHHPA010000173 DAHHPA010000000
VERSION     DAHHPA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.181"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            33..689
                     /locus_tag="NBH55_002783"
     CDS             33..689
                     /locus_tag="NBH55_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1510704.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            689..2065
                     /locus_tag="NBH55_002784"
     CDS             689..2065
                     /locus_tag="NBH55_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1510705.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2351..>2870)
                     /locus_tag="NBH55_002785"
     CDS             complement(2351..>2870)
                     /locus_tag="NBH55_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1510706.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         1091 a          385 c          509 g          885 t
ORIGIN      
        1 aaatcttatc aaaaataagt taggagaaaa taatggatat attagaaata aagaatgtag
       61 catatagtta tgcaaattct aaagaaaaag ttttgtcagg agtaaatcaa aaatttgaac
      121 ttgggaagtt ttatgcgata gtaggaaagt caggaacagg aaaatctaca cttctttcct
      181 tacttgctgg acttgataaa cctcaaacag gaaaaatatt gtttaagaat gaagatatac
      241 aaaataaagg atatagtaat catagaaaaa ataatatatc tttagtattt caaaactata
      301 atttaataga ttatttatca ccaattgaaa atattagatt agtaaataaa tcagcagatg
      361 aaagtatctt gtttgaacta ggtttagata aaaaacaaat aaaaagaaat gttatgaaat
      421 tatctggtgg acagcaacaa agagtagcta ttgctagagc attggtatca gatgctccaa
      481 taatactagc tgatgagcct actggtaatc tagacagtgt tactgctgga gaaataatta
      541 atatattaaa gacattagct aaagacagaa ataaatgtgt aatagttgta acacatagta
      601 aggaagtagc agattctgcg gatatcattt tagaactaag tggtaaaaag ttgaaaaaag
      661 taaataaaat gaatttggag gttgaataat gataaaaaat gcatttgctt atgtaactag
      721 aaaaagttta aaatcattaa ttatcatatt agttatttta tctatgtcaa ctttaagtct
      781 cattagttta tctattaaag atgctacgga cagagcttca aaagaaacat ttgctaatat
      841 aacaaatagt ttttctatgg agataaatag acaagtaaat ccaggaacac ctagaggtgg
      901 gggaaatgta aaaggtgaag atattaaaaa gatatctcaa acagatagta tagactccta
      961 tgtaaaaaga ataaacagtg ttgcagattt agttgattat gatattattg aaactcaaga
     1021 gactcttgcg aatcaatcac ctgaaagagc aaagaatttt aaaagaacag ttatgttaac
     1081 tggggttaac gactcaacaa aagaaacaaa atttgtatca gaagcatata aattagtaga
     1141 aggaaagcat ttagaaaatg aagataaaaa taaaatctta atgcataaag atttagctga
     1201 aaaaaataac cttaaagttg gagataagat aaaaataaaa tctaatttat ttgatgctga
     1261 taatgagaaa ggtgcagatg aaacagtaga agtagaaatt aaaggtctat tcgatgggca
     1321 taatagcggt gtagtaagtg cagcacaaga actatacgaa aatacattaa tcactgatat
     1381 tcatacagct gctaaagttt atggtaatac cgaagataca gctgtatacc aagatgcaac
     1441 attctttgta aaaggtgata agaatcttga tagtgtaata aaagatcttg ggaaattaga
     1501 tataaattgg agagaatata atttgattaa aagttcatca aattaccctg cattacaaca
     1561 atctatatca ggtatttact caatttcaaa taaattattt tttggctcat taatatttgc
     1621 aggagttgta gtttcattat tattattctt atggatgaat gctagaaaga aagaaatagc
     1681 agtattacta tcattaggta tatcaaaatt agaaattttt ggtcaattcc taattgagat
     1741 ggtatttata tcaatccccg cattcgttgg ttcctatttc ttagctcaat atacagctga
     1801 caagcttggg aataatatat tgaataaggt tactggtgat atagctaaac aaatagctaa
     1861 acaatctgca tctagccaat taggtggtgg ggcagaagct gaaggattta acaagacatt
     1921 atcaagttta gatatcaatg tattacctaa attcatgatt tatgtagtta tatttatgag
     1981 tttagtactt cttgtatcat tgattatttc ttcattcaat atattaagaa ggaatccaaa
     2041 agaattatta attgacaata attaaaattt tcggtgcttt ttagcaccaa aaatgagagt
     2101 gtaatataaa ccgtgtaagt aaccatcaat cctaggcggg tggttgcttc acggttttta
     2161 tttttctaaa ataagatcaa gaggtgatta cacgatggct agaaagaaaa gaaatcctga
     2221 tgctgagaag ttagctgaat ccattttgaa tacttatcaa cccgaatctg tagaggatat
     2281 gcaagatgtt ttaaaagatg tgtttggtcc cttatttgaa aaaatgcttc aaggagaatt
     2341 gaataaccat ttagggtatg acgcccattc taaagagcca aagaaacatg acaatcgacg
     2401 aaacggttat ggcaataaga cgcttaaaac gagctttggt aaagtaaaca ttgatgttcc
     2461 cagagaccgg gaggcttctt ttgaacctga gttgattcct aatagaaaac gagatgtctc
     2521 cgacatcgca gactgtagac aaaagctgtt ccaaactcgt aactcgagct tggaacagct
     2581 ttttatctac ttcgtttcga atttttgaac aaaaaaataa aggctccttg cctttccata
     2641 gccaggtggc taatttcttt aaattcattg cagcaaacac tagcatcgta tgcgtcgcga
     2701 ctttttccaa gcctcgatac ttggtccaac gcatcccatg cttttctttt gcatccgcaa
     2761 atattcgttc aatcgtttgt ttccgttttt tgtaaattga tttattcaga tctgttaggc
     2821 gaagatgttc cacttcatcc atcagatctc cccaaacatg tctcgtgatg
//
LOCUS       DAHHPA010000174         2780 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.182, whole genome shotgun sequence.
ACCESSION   DAHHPA010000174 DAHHPA010000000
VERSION     DAHHPA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.182"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..197
                     /locus_tag="NBH55_002786"
     CDS             <1..197
                     /locus_tag="NBH55_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510707.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="NBH55_002787"
     CDS             314..811
                     /locus_tag="NBH55_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCD1510708.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            786..1292
                     /locus_tag="NBH55_002788"
     CDS             786..1292
                     /locus_tag="NBH55_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1510709.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1276..>2780
                     /locus_tag="NBH55_002789"
     CDS             1276..>2780
                     /locus_tag="NBH55_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1510710.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          515 c          600 g          826 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
      481 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
      541 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
      601 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
      721 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
      781 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
      841 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
      901 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
      961 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     1021 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     1081 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     1141 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     1201 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     1261 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     1321 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     1381 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     1441 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     1501 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     1561 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     1621 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     1681 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     1741 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     1801 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     1861 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     1921 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     1981 gaagaaaaac agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     2041 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     2101 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     2161 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     2221 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     2281 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     2341 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     2401 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     2461 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     2521 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     2581 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     2641 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     2701 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     2761 gtgattgtag acccaaaagc
//
LOCUS       DAHHPA010000175         2779 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.183, whole genome shotgun sequence.
ACCESSION   DAHHPA010000175 DAHHPA010000000
VERSION     DAHHPA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.183"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            188..511
                     /locus_tag="NBH55_002790"
     CDS             188..511
                     /locus_tag="NBH55_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510711.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            675..929
                     /locus_tag="NBH55_002791"
     CDS             675..929
                     /locus_tag="NBH55_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510712.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            999..1244
                     /locus_tag="NBH55_002792"
     CDS             999..1244
                     /locus_tag="NBH55_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1510713.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1341..1685
                     /locus_tag="NBH55_002793"
     CDS             1341..1685
                     /locus_tag="NBH55_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510714.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1746..2309)
                     /locus_tag="NBH55_002794"
     CDS             complement(1746..2309)
                     /locus_tag="NBH55_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510715.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          822 a          481 c          428 g         1048 t
ORIGIN      
        1 catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca
       61 caagtttcaa aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata
      121 actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt
      181 attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc
      241 ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat
      301 gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa
      361 aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat
      421 catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt
      481 agatatcaat ccattgattt tttctcgata gataagacac attcagcgga acagttcctg
      541 cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt
      601 tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt
      661 gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac
      721 tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt
      781 tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg
      841 atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt
      901 atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata
      961 gtcattataa ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt
     1021 tactggatac tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag
     1081 tagctgtttc tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag
     1141 aagaaattcc aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg
     1201 attacctaat tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg
     1261 tgctggtttt ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta
     1321 tgtgattatg gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac
     1381 atcgggaatg ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt
     1441 atgtttttca ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa
     1501 tggaaaaacg tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat
     1561 catgcgaagc attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac
     1621 acgatgaagt ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta
     1681 cctaatataa ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc
     1741 ttagatcaag ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta
     1801 aaagcgtact ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg
     1861 acgtggttta aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt
     1921 cttttgatct ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat
     1981 cgatgcttac aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag
     2041 tcactgtgat cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc
     2101 agttcagttg ctaagccaga gtcagaacaa cctgccattg ctagcgttaa tattgatagt
     2161 aaaaatagtt tttttatcat aaggttcccc ccctgaaaat agactaaaat aataacgctt
     2221 tctttttttg ttttcagacg aaaattaata atttctaaat attctttaaa aaagcttata
     2281 actattatgc cttttttaat atataacaca gttattttta aaaataagaa tgctaaatca
     2341 ctctacaata acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag
     2401 atgaaagatc aggttttatt agatctcatt ctttcatcac ttgaagggat ttaatgaact
     2461 actttttcct ctttgtcgtt tttatatatt cgtattcctt caaacagtag gatagcctct
     2521 ttcaatgctt gatatttgaa ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc
     2581 tagaacatat tcttttttac tttttcatta attgacagac caatcgaacg aaactgtaaa
     2641 tttcgatagg ataatgattg gtcaaactca ttcattttca agacatctcg attcatattc
     2701 aagataggaa tctttttatc aacatcactt tattcatatt ttcctctacc ataaaagctt
     2761 cacgctccac aaaattgtt
//
LOCUS       DAHHPA010000176         2772 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.184, whole genome shotgun sequence.
ACCESSION   DAHHPA010000176 DAHHPA010000000
VERSION     DAHHPA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.184"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(64..306)
                     /locus_tag="NBH55_002795"
     CDS             complement(64..306)
                     /locus_tag="NBH55_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD1510716.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(338..895)
                     /locus_tag="NBH55_002796"
     CDS             complement(338..895)
                     /locus_tag="NBH55_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1510717.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(908..1096)
                     /locus_tag="NBH55_002797"
     CDS             complement(908..1096)
                     /locus_tag="NBH55_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCD1510718.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1109..1675)
                     /gene="amaP"
                     /locus_tag="NBH55_002798"
     CDS             complement(1109..1675)
                     /gene="amaP"
                     /locus_tag="NBH55_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCD1510719.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2092..2265
                     /locus_tag="NBH55_002799"
     CDS             2092..2265
                     /locus_tag="NBH55_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510720.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          806 a          532 c          406 g         1028 t
ORIGIN      
        1 gcgagtgtcg ctacggttct caaatttgtt cttcatttca acataagttt ttcttcaatt
       61 tgcttataat ttacgtgcaa agaatgaaac gactaatact aaaatgattg caccgatcaa
      121 tgatggaatg atagccattc ctgcaattgt tggtccccaa gttcctagta aagcttgtcc
      181 aattgctgaa cctactaaac cagcaatgat gtttgcgatg atccccattg atgatccttt
      241 attcgtgatt gctcctgcaa ttgctccaat gataccgccg acgattaatg cccaaataaa
      301 tcccatttta aattcctcct aactgacttc ttacttctta ttgtacacgt ggttcgttat
      361 tttctttgac tttttgtgca cctttgccta cagcagattt tgctttatct gtttggcgtg
      421 aagcaaattg tcctgtggat tttgctgctt ctgttacttt gtcttgtact gtttcttgat
      481 ctttttggta ttcttcttgt gttttgatgt ctgcaacatt cacattcact tcgataacat
      541 ctaaatgtgt catattgttt acttcagtac tgatcaattc tttgatttgg ttgtaaatat
      601 cttcgatgtc tttaccgtat tctacgatca catctagatc cacagctact tgtttcttac
      661 ctacttctgt attgatacct gctgttacgt tatctgtgtt gactaatttt tcagccatat
      721 ttgagaaaaa tccgccatct aatgtcaata gaccatctac atctttcaat gcataaccga
      781 tgattttttg gatgactttg tcttcataag tcaattcacc attcatcatg cggttttctc
      841 ctttttcatt tgccaaaact gatccatttg ttgtattcat gtttttgttt tccatctttg
      901 tttcctccta tgatttaaaa aattgatcaa ctattcctga ctgtttcaaa taaaaaccga
      961 ttcctgcgcc aattgctgtc atgattatga tcaacaatgt cttgaaaaat ccaatcgtta
     1021 accataaaac agctagcagt agtccaagac ccccaaagat aatagggagc ttgtatgctg
     1081 aaaatagttc tttcattatt tggccctcct attccactcg agagtgcttg tagtctcttg
     1141 tatcttcttg ttcttcataa ccgctgtatg tgactgccac tttgaccgtt tcttttgttc
     1201 cgagtacttt agttacttct tcttcgatat cttgcatcaa tacacctgtt ttccctacta
     1261 atgaagaagt tcgtttcaag tctccgttga catggatatg gattcgattt ttcgttgaac
     1321 gtatccgtac tttaggtgaa tgaatgaact cttcttcgtg taaatgggaa cgtaccaacc
     1381 cttcgatcgc ttttttgtcg attgttagtt tgccatcttc ccgtttcata acgaaagttc
     1441 ctcttgactt tggatagaaa agaacgacta gcaagatgat aatagcaaga acggcaaata
     1501 ctacagctgc ccagaaaata tattgttgca tgtagtaatt cgttaaagta aagaagcgaa
     1561 atcgttccaa ttgttcaggc ataatatacg gcgcattgat caacgcaatc gctagaaata
     1621 ctgaaagtag cagtaaactc atgataacac cgattatttt taacccctta ttcattttcc
     1681 acctcctata aacttatttt taccctgtaa ataactctac cgacaatcac agtatagata
     1741 gcgaacagtt agcatagcat cagtaaataa agcgctacca aacattgctg gatcataacg
     1801 gcgttttatt gccagtctga tactgaaata acaattgctt acttattctc tatttacttt
     1861 tgattgctgg ctatacttaa aggatattat tcaagattaa aaattgcaaa gattatgctc
     1921 agttattatt acaagattct atttatttat taaatttttt cggagaatta aaaaatacaa
     1981 taaataactt tagttacttt aatgcgttct tactttattt ttatattatc atttttctta
     2041 ttcacttctt tggtttatac tattcttata aataaaaacg aggtatgtcc tatgatgaaa
     2101 agattgtact attcattgat cataacgatt ggctatttga ttgtatcaaa tctaggaaat
     2161 atggtatttg gtatcagtaa agaattcagt tggactacta cattatggga atctctattc
     2221 tttttcatct tcgtattttt gttacaaaat tatcgaaaaa aataaacatg tgccatctat
     2281 ctgttggcac atgttttagg atttatggta ctttttattc ggcaatccat tttatttttc
     2341 tacttcttct taatacctct aacacttttg atttataaac taatccaatg catctacttg
     2401 cgcttattaa gctatttcag ctagatagta ccaccccatg cgtcaatgct tgattttcct
     2461 tttgtataac attgtataag caataaagga caaataagtg gtgattacac caaaccattg
     2521 aataagaaaa attctgatac gtaaataaac atgaacttga aaaaattaat gaatcggtta
     2581 gctactacgt agttgtcttt tttctcatac agttctgtta acttacactt ctcttcgcta
     2641 gataataaaa aatcctccaa ctgtattgtc caatataatc ccaggcatct aaactagcat
     2701 tatagaagag acacacatag aatctaaaat aactggtttt aaatgtttct tacaatcaaa
     2761 aaaagctgtt aa
//
LOCUS       DAHHPA010000177         2763 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.185, whole genome shotgun sequence.
ACCESSION   DAHHPA010000177 DAHHPA010000000
VERSION     DAHHPA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.185"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..262
                     /locus_tag="NBH55_002800"
     CDS             <1..262
                     /locus_tag="NBH55_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1510721.1"
                     /translation="SNEVLYKEQVLPIQGKEFELLVYFLQNKGIILTKEQIFDRIWGF
                     DSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVGYILQRV"
     gene            267..1607
                     /locus_tag="NBH55_002801"
     CDS             267..1607
                     /locus_tag="NBH55_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCD1510722.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(1756..2742)
                     /locus_tag="NBH55_002802"
     CDS             complement(1756..2742)
                     /locus_tag="NBH55_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCD1510723.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVREDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT          826 a          543 c          490 g          904 t
ORIGIN      
        1 aagcaatgaa gtcttgtata aagaacaagt tttgcccata caaggaaaag agtttgaatt
       61 gctcgtttat tttttacaaa ataagggaat cattttgaca aaagagcaga tatttgaccg
      121 gatctgggga tttgattctg atacgacgat tacagttatt gaagtttata tgagcaactt
      181 acgaaaacat ctgcgaagta ctggattgga taaagaaatc aaaaccttac gtaatgtcgg
      241 ttatatactg caaagggtgt gatgcaatga atggagatat tacacgaaag caacgtagtc
      301 gcttttttgc gacaaatgta gcagcatttg cttttatctt ttttcttttg ggattcatta
      361 ctttacagat attaaaccaa tctgcataca gagaaacaga tctgtcatta gagaaaataa
      421 attttgattc tcgaacagta caattagaga tacaaagata tcagcaaggc gatccctttt
      481 taaacccgac tcaaacggaa caaaacagaa caaacatgga acagccatta atggaaccat
      541 caaatgaccg ttttaacact caagtgatct tgtggtctaa aaatggtgag atattgaata
      601 aagcagctat tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc
      661 tgaataccat ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc ctctctttcc
      721 gttcgattac gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc caaattttag
      781 aaaatacaaa tcaaatcaag aattcattag cgacttttcg cacgattatg attttgtgta
      841 tgattgtttt ttggattctc tctattggta tcagttatta cttgtctagt ctaagcatgc
      901 gccctattat tctttcttgg agaagacaaa aagaatttgt tgagaatgct tctcatgaat
      961 tgcgcacacc gttaacgatt attcaaaata gtttggaaca tctttttacc aagcctaatc
     1021 atactattat tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca
     1081 ctggattgac ttcggatttg ctgacaatcg ctcggaatga ttctgatcag cagctgcttt
     1141 caaagcaaat gatcaataca caagaatata tcgaacaact tgttaaacca tttcaagaga
     1201 tggccatcat agatggaaaa gagtttattc tccataacga tgcaacatta gaagtaccaa
     1261 ttgatgaaaa aaaaatccat caagttttgg ttattttgct ggataatgca ttaaaatata
     1321 cgaatccaga agataagatc attttggatt catctgttaa ccataaagaa tggctgattc
     1381 acgtaaagaa tactggccct tctatcagtg aagaagatca agcaaggatt tttgatcgat
     1441 tttatcggga agatcaatct cgggcgaaag agactggcgg atacgggctt ggcttggcaa
     1501 ttgcaaaaca ggtaatcgaa cagcatcatg gacagatttc tgtatccaat tatcttccac
     1561 aaggagtcga ttttaaagtc caattgccgt tgaagcctaa aaaataatgc ttatccctaa
     1621 aaaagggctg tgacaaaaat cttgtcacag cccttttttg ttgcgaataa acggtgttcc
     1681 agaagtagct tcttcggaaa taagccgaaa tttctttaag ctgaccactt ctgtcacaac
     1741 ctctttagct tacgcttacg attttttatt gcggtcttca atgtgtacat caccgcttgt
     1801 gtcaatatcc aattcttctc ggctaacatc ttctctcact ttttcaacat cttcatgttc
     1861 ttctttatga atcccaactt cttcacgaat aactgtatgt ttgcttacat ctacttgctc
     1921 ttctttgatt gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga
     1981 ttgatctgtt actggtttgc gttcgatgac gatttcttca cgtttgaccg gtacttctac
     2041 cgtttcagta tcgttgacca catgtttgtg gatgtcgact tctccagttg tcacatcatg
     2101 tgtatttaca tctaattttt cttcttttag ttgaattgta tcatcatcta gatttggttt
     2161 gcgatcgatg tcttcagtct tgcttgtacc cattgtatca gtctcgccag gtacgtccat
     2221 cggatcagtt cctggtgtca tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc
     2281 acctacgtcc attggatctg ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc
     2341 tgggctaaca gtatcttgtg tatccattgt atcttctgtt gtttctggct gacgatagcc
     2401 ttttactgtg atgacaaatt ttccatcaga aatatctcct ctatattgat ataaaggatc
     2461 attttctggt gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat cttttatttt
     2521 ttcccataaa gaacggtctt cttcaccatc catccgattt tcatctgttt caacatctat
     2581 accagacaat ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat aaataacgat
     2641 atcatctcgt tcataccctt cgttacgcaa ccgttcaatt actgataatg cttcttccct
     2701 cgtactatag cttccgatga ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa
     2761 ata
//
LOCUS       DAHHPA010000178         2749 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.186, whole genome shotgun sequence.
ACCESSION   DAHHPA010000178 DAHHPA010000000
VERSION     DAHHPA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.186"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            142..1209
                     /gene="rlmN"
                     /locus_tag="NBH55_002803"
     CDS             142..1209
                     /gene="rlmN"
                     /locus_tag="NBH55_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCD1510724.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1402..2505)
                     /locus_tag="NBH55_002804"
     CDS             complement(1402..2505)
                     /locus_tag="NBH55_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD1510725.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT          868 a          491 c          551 g          839 t
ORIGIN      
        1 ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa gttctattcg
       61 atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta gcgaaacttt
      121 gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac gaatgaagaa
      181 ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca agtttgggaa
      241 tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc gaaacaattg
      301 atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt cgtccaagaa
      361 gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat gattgagact
      421 gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt aggatgtaat
      481 atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct gactgctgga
      541 gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa tcttggcgaa
      601 cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta cgacaatgta
      661 atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc tcggcacatt
      721 acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa cggcttgcaa
      781 gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg
      841 atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta tttagagaaa
      901 acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga tagaccagag
      961 catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata tgtcaacttg
     1021 attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa agctgatgta
     1081 ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat
     1141 ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt
     1201 gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag ttttcttaat
     1261 taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta taaagctttt
     1321 tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga cagcctgctt
     1381 gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc tttcttgtgt
     1441 agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg tgatttcata
     1501 aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt ctaaatcggt
     1561 cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt tcataaacac
     1621 atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca gtacaccata
     1681 actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg taccgtaata
     1741 gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa ccaatccggt
     1801 agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat cgtcggcact
     1861 atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt ttccgatttg
     1921 gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag tagattcgac
     1981 ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg cttcggcaat
     2041 aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat
     2101 tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat
     2161 agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta cagtatcgca
     2221 agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt catgctggat
     2281 caactgttca atatttccat aagttgaatt acccccgtac cagagactgg cgacaacctc
     2341 tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg cagtcttccc
     2401 tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt tttcatacgc
     2461 atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca tttccttctt
     2521 tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg ctgtagatgg
     2581 agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac accttgaaat
     2641 catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac aataatgaat
     2701 ttttgttcct cttagtattt ctaccgattc cctttgcttt tttgtaatt
//
LOCUS       DAHHPA010000179         2746 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.187, whole genome shotgun sequence.
ACCESSION   DAHHPA010000179 DAHHPA010000000
VERSION     DAHHPA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.187"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..443)
                     /locus_tag="NBH55_002805"
     CDS             complement(<1..443)
                     /locus_tag="NBH55_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD1510726.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="NBH55_002806"
     CDS             513..863
                     /locus_tag="NBH55_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1510727.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="NBH55_002807"
     CDS             1277..2095
                     /locus_tag="NBH55_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1510728.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="NBH55_002808"
     CDS             complement(2168..2527)
                     /locus_tag="NBH55_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCD1510729.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAVGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          878 a          420 c          517 g          931 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc cactgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctat
//
LOCUS       DAHHPA010000180         2643 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.188, whole genome shotgun sequence.
ACCESSION   DAHHPA010000180 DAHHPA010000000
VERSION     DAHHPA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.188"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="NBH55_002809"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="NBH55_002809"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCD1510730.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          951 a          461 c          584 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
     2641 aaa
//
LOCUS       DAHHPA010000181         2620 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.189, whole genome shotgun sequence.
ACCESSION   DAHHPA010000181 DAHHPA010000000
VERSION     DAHHPA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.189"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            128..1759
                     /locus_tag="NBH55_002810"
     CDS             128..1759
                     /locus_tag="NBH55_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCD1510731.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLIIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1762..>2620
                     /locus_tag="NBH55_002811"
     CDS             1762..>2620
                     /locus_tag="NBH55_002811"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCD1510732.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLD"
BASE COUNT          914 a          364 c          536 g          806 t
ORIGIN      
        1 aaaatacaat cattctgata tggtagaaaa aagcaagttt attccctttt tatattgaca
       61 acggttacaa cgtgttttaa aatataaaat gtatcaaaac taacatatca gtttgaggag
      121 gttaattatg gtttcgatta aaaattggca aaattctatc gaagaattca atgaaatgca
      181 gattctactg ccgtgttttg atgtcaaaga agttaagcaa gaaggccagc aacatccagt
      241 atggcttcat tttggtggag gaaatcttta tcgaggattt catgcagaaa ttgctcaatg
      301 cttagcagat caaaatgaat tagaaacagg agtagttgtg tgtgaaacat ttgatgaaga
      361 agtagtggaa aaagcttatc atgactataa caatgacatt ttggaaattg ttatgagtgg
      421 agatggtgaa ctcaaaaaaa gacttttggc agtaacggct gatagtttat attgcaatcc
      481 gaagaatata gcggactatc aaagagtggt taaatatttt gaagatccta aattacagtt
      541 tacaacgttt actattactg aaaaaggtta cagtttaaaa gattctaaag ggcaattctt
      601 ttcggtagtc aaagctgata tcaaatctgg acctagcaat cctcaacata cgatgagtat
      661 tgtaacatcc ttgttatata gcagatttaa tgctggagag ttgccgattg cgatggtttc
      721 tactgataat ttctcacaga atggtcaacg ttttcaagat agtattttta cgattgcaga
      781 agcttggcaa aaagaaggat atgtttctga aaaatttatt gcttatttaa aggataaaga
      841 aaaagtgact tttccttgga gtatgattga tcgaattacg ccaaatcctt cagagaatgt
      901 aaaggaagat ctattgatga tggggctcga ggatttagat atcattcaca caaaaaaaca
      961 taccaatatt gctgctttca ctaatactga ggtagttcat tatttaatta ttgaagatag
     1021 ttttccaaat ggccggccta aattggaaaa ggctggtgtg attatgacag atagagagac
     1081 ggttgataaa gcagacatca tgaaggttac gacaagcttg aatccacttc atactgcttt
     1141 ggcagtaaca ggttgtctgc taggatatca atcaatttca gcagagatgc aggatgatga
     1201 tttggttggt ttaatcaaag gaataggtta tttagaaggt ttaccagtag ttgaaaatcc
     1261 aggcattatt gatccaaaac aatttattga tgaagtaatt aataaacgtt tgcctaatcc
     1321 ctatattcca gatacaccac aacgaattgc aacagatact tctcaaaaag tagcgattcg
     1381 ctatggtgaa acaattaaaa agtatgtcga aaaagacgga aatgcagaaa atctaacatt
     1441 tattcctatc gctattgctg cttggttacg ttatttactg gcagtcgatg atcaagggga
     1501 aaattttgtg ccaagtcctg atccgttatt ggtagagttg caggataaat tatcagtatt
     1561 gaaattaggt gaacaagatc cttcaaagat tcattgtgcg gtacaggaaa ttttagaaaa
     1621 taaagaaatc tttggtttag atttatatca agttggggta ggaaagaaaa ctgaaaaaat
     1681 cttttgcgaa atgctagtag gtcctcaagc agttcgtaaa acaatcaata aatatataac
     1741 agaagttgga ggaaagtaac tatggaaatg acttttagat ggtatggggc aaatgaagaa
     1801 aaaatctcac tacaagaaat tcgtcagatc ccagcaatga aaggaattgt tggaacatta
     1861 atggatatac cagccggaga ggtttggccg agagaacgaa ttaaatcttt aaaagatgaa
     1921 gtcgaagcca atggacttac cttgaaagtt attgagagtg ttaatgttca tgatgatatc
     1981 aaaattggtt taccaacacg agatcaatac attgagaatt ataaacagac gattcgcaat
     2041 ttggcagaat ttgggattga ggttatctgt tataatttta tgccagtatt tgattgggta
     2101 aaatcggatt tggattatcg tttagatgat gggtcttcta cgttagccta tatttcaaaa
     2161 aatatcccta ctgatccgga ggaaattatt agaaaagtag aaaattctac taataatttt
     2221 gaattgccgg gttgggaacc tcatcggtta tcacaaatta agcaattatt agaagcctat
     2281 aaagaagttg atgaagagaa attacgagag aactttgcat attttctcaa agcaattatc
     2341 ccgacatgtg aagaagttgg gattaaaatg gcagttcacc cagatgatcc accatattcc
     2401 attttcggat tacctcgtat tgttaaaaat cgtgacgatt tagattggtt gtgtaatgta
     2461 gtggattctc cttctaatgg tattacatta tgtactggaa gtattgcaga acgaccagat
     2521 aatgatgtct atgaaattct atctgaattt tgtcaacggg atcggattcc ttttgcacat
     2581 gttcgtaata tcaaatattt aagtgacaat ggactcgatt
//
LOCUS       DAHHPA010000182         2476 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.190, whole genome shotgun sequence.
ACCESSION   DAHHPA010000182 DAHHPA010000000
VERSION     DAHHPA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.190"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..381
                     /locus_tag="NBH55_002812"
     CDS             <1..381
                     /locus_tag="NBH55_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510733.1"
                     /translation="EIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFAN
                     TPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNY
                     IVKAKVSKRFIFNPKLEIYSVVIE"
     gene            706..1323
                     /locus_tag="NBH55_002813"
     CDS             706..1323
                     /locus_tag="NBH55_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1510734.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1364..1663
                     /locus_tag="NBH55_002814"
     CDS             1364..1663
                     /locus_tag="NBH55_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCD1510735.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1697..1864
                     /locus_tag="NBH55_002815"
     CDS             1697..1864
                     /locus_tag="NBH55_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1510736.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1909..2472)
                     /locus_tag="NBH55_002816"
     CDS             complement(1909..2472)
                     /locus_tag="NBH55_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1510737.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          859 a          377 c          469 g          771 t
ORIGIN      
        1 gaaatagaat ggaataatct gactcaaaat aataaaaata attctggatg tggaattatc
       61 tttatgtttg ttatagtttt gggaatagta gtacttttaa taactgtcaa gatagtagat
      121 tactttgcga atactcctga agaacaaata cagagaaaac ttcctgagtt tgaaagagta
      181 ccaccttatg atccaagaga taattcattt caagtaaaaa aattaaaaga gtctccaaat
      241 gaaatgtggt ttgaagttac agttgaatca agcgacttca aagaaaaaaa gaaagaatac
      301 aattatattg taaaggcaaa agtatcaaaa agatttattt ttaaccctaa attagaaata
      361 tatagtgttg taatagaata atagtgttaa aaaaattgta aaagttaaag aagtcaaatt
      421 ctgatgtgaa aaataaaata taccatggaa gacataaaag aaaaactgga taactagttt
      481 atttaatggc agaccgttaa ggcctgccat ttttatgtat tttagcggaa taaatataat
      541 catgcgggag tgtaaactta ctgtttcaaa aagtatactt tttgacatgc cttgattttc
      601 tttgtttatg tatggcataa taaccacata atacaatttg aaacgtttct gatttaatct
      661 gattgttttg aaacaatttt agtgaaaaaa gggtagataa aaacgatgag aaaatttttt
      721 tatatccggg tcagtagtta tgatcaatcg atcgaacgac aactgatttc cgcgaaggaa
      781 ttaggaatcc ctgaagaata tatatttgta gaaaaagcca gcggcaaaga ttttaaacgc
      841 cctgaatatc aattaatgaa acggatgttt cgaaaaggag atttactcta tctacaatca
      901 ttagatagac taggcagaaa taaacaaatg attttagaag aatggcgaga attaactaag
      961 gaaaagcaga ttgatatttc tgtgttagac atgcctctgt tagatactgc tagttacaag
     1021 caactagatg ggttagagac gcttatctct gacttaattc tgcaattact cagctatatg
     1081 gccgaagacg agcggaaacg aattaatagc cggcaaagag aaggcgtaga agctgcgaaa
     1141 caaaagggtg taaaattcgg cagaaagaaa attgctttac ctcctgaatt tcctgcgatc
     1201 tattctgact ggaaagcagg aaagataact gcagtctatg ctatggatca attgggcatg
     1261 aaacgaaata ccttttatcg acgggtgaaa gaatatgaac ataccttgca ccacttttct
     1321 tgatagatat aacagctatt agacgggaag ggagagtttg aaaatggact taacgaatca
     1381 aagggtcgtt tttactggaa cgctgcagca aatgacacga acccaagcca tcggtttggt
     1441 tcactcttta aacggccatt gtcaatcaag tgttacttca aaaacaaatt tcctggtttg
     1501 tggtcagtat tctaagtcgt tgtttgatga ttctctctat acaaaaaagg aacaaaccgc
     1561 gcgggacttg attgcgttag gtcatgagat taccatttta tcagaagtcg aattttatgc
     1621 gttaattagt caacagttga aagaatggca acggtattta taatcgttag taaaatttac
     1681 aaggaggaaa atttttatga agcgaaaatc ttatgatcgt tggggcattg aaataactag
     1741 ttttaatcga aatattgctt ttcacgtgga gtgtgactgt ggggaattag caaaaagtgt
     1801 ttatcaaaaa aattactttt actgtcctaa atgccatcgc cgatacgggg tacatggaac
     1861 atagaaacga aatagtgcgg tagaaaagcg aatgaatgta gtatatttct acaaatcatt
     1921 cgctttcttt attttataaa tagttggtcg agagatcccg ttttcttttg caatctctgc
     1981 aactggaatg ccttgcttta acatgtccac cacggtttta aaaactaagc gtttttgcgg
     2041 atctgttgag ttaatagaat actcgattgg tcgtccctta tatcggccgg ctttttgagc
     2101 caataatact ccttgccgct gacgttcacg aattttctca cgctcgtttt gtgcaatata
     2161 acttagcagt gataaaaaca tatcgaacat agcggtatcc aggagttcat ttccagtatt
     2221 aaaatctaaa aactgtgcat ctaatatttt taacgctact tttttctgtt tcataaaagc
     2281 aactgtattt tttatatctt cataatctct tcctaaacga tctagtgatg taacgatgac
     2341 gcaatcacct tcacggatat aatttagtaa tttttgaaac tcaggacgct cagtatcttt
     2401 accactggct ttttcttgga ataatttatc aatgttaagt ccttcggttc tgttgcgaag
     2461 tttgaaaatc aaacgc
//
LOCUS       DAHHPA010000183         2443 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.191, whole genome shotgun sequence.
ACCESSION   DAHHPA010000183 DAHHPA010000000
VERSION     DAHHPA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.191"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            13..495
                     /locus_tag="NBH55_002817"
     CDS             13..495
                     /locus_tag="NBH55_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCD1510738.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            532..1542
                     /locus_tag="NBH55_002818"
     CDS             532..1542
                     /locus_tag="NBH55_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCD1510739.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1657..2370)
                     /locus_tag="NBH55_002819"
     CDS             complement(1657..2370)
                     /locus_tag="NBH55_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510740.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          826 a          403 c          499 g          715 t
ORIGIN      
        1 agagacataa aaatgattga cttggcaaag aaattcttac acgaaaatat atattttgag
       61 ttgtatcatc atcgagccat ttatacaaat gaagatgctt tggtaataaa gaaagaacaa
      121 ggatttacag gtacagagac caaaagctta ttcttaaaag ataaaaaaga taataactac
      181 gtatatctga cttttactac gaaacaaacg gatttcaaag cattagggaa gatcgtcggt
      241 aaaaggcttt ctattgtttc atcagaacgc atggaagaac aaacgggaca aaagcagggc
      301 gctgtttctc cgtttggtta tgaaaaacag attccgatta ttattgatga agagctctta
      361 tcaaatgaaa aacttgtttt tgcaccaggt agaccagatc aaacgatggt catccaaacg
      421 gttgacttaa acaaaatcat ccagctactt gagatgccga tatttttgat gccggcaaac
      481 gaaaaaaaca attaaccaag taaaatagtg catacttgga ggtgaagcaa catgataagt
      541 tatccattat tagaaagaaa tgcaacgatt ggggttactg caccatcctc aggcgtatca
      601 gaggaacacc attcgttact cgaatcagct ttaaagagaa tggaagaaaa atctttccaa
      661 gtagttactg gaaagacgct ttggacgcaa gataaggaaa aatcaggtcc agcaagaaaa
      721 cgagcagatg aattcaatca aatgatgcaa gatgaaaata tccaaatgat ctttccgcct
      781 tggggaggag aattattgat tgagattctc gattttgtcg attttgatgc tctacaacca
      841 aaatggatag taggttattc agatattagt acgttgctac ttgctgtcac attaaaaaca
      901 gggatagcca ctgcacatgg aaccaacctt attgatttaa gaggagagta cttggatgaa
      961 acgacagcaa tgtgggaaag tgtactgaaa acaagagaag gagaatcgat tctacagcaa
     1021 ccttctgaaa agtatcaaaa agagtggccc catgagcatt catcatcttc tgttttccat
     1081 ctcactgaac caacaaattg gcaatcagtc agcggagatg cagtaaggat gaagggacga
     1141 ttattaggtg gctgtattga tacgatcagt catttaatcg gtacccctta tggagatgtc
     1201 caatctttta aaagaaagta taatgatgga aagccgatct tgtggtttct agaaaattgt
     1261 gagctatcgt cagttgattt aagaagatca cttgttcaga tgaaactggc aggatggttt
     1321 gatggcagct cggggatcat gttcggtaga agtgcagcag aggatttgtt agattataaa
     1381 atgaaggatg tatatgaaga tctgtcaaag gaattaaaac taccgatctt gtatgatatt
     1441 gattgtggtc atgtaccgcc acaactgaca ttgatcaatg gtgcctatgc agaagtagcg
     1501 gtagaagata gaaaagcgac tgttttgcaa acgtttattt aaaaaatttt ttcctgcctt
     1561 ggagtttatc aaagaccaac aaatctaaat agaaaagaaa atgaaagaag ctgacagtat
     1621 tttgccagct tctttttgta tgatctatta gaaattttaa ttatccataa ctatcacatt
     1681 gatcagctgt ttcacatcat ttggattacc tgtaggaata gctactacag atgcattatc
     1741 aataatttct tgagttgttt ctctaatgaa cgctggataa gatgtttgat ctgcaggcat
     1801 ttgccaatgt gaaggaactg gataactagt gattgtcccg tcatggttac ttgaatacac
     1861 taaaactccc tggtaactgt atttcccagt gagcataacc acatctttcg gatagactgc
     1921 actccctgca tcataagggg cgataggtgt tcctgcagga taatgaataa catgtagttc
     1981 gaaatcaccc gatgtatctc taaaagtatt acctctaatt gctagccaaa cacgtgcgta
     2041 ctcaatttgt agatcagaat atcctgctaa cggatctatt gactgagaag actgtgagga
     2101 agttgtgtct attgttgaag aagaaatatt tgtctcaact gtactactag atgtagaaga
     2161 agtattgggc tctgagactt tactgcttgt tgaagatgta ttcgtttcag tagaagctga
     2221 tttctgagta cttgattttt cagctgaact ggtttgtttg attgaacttt tgtctgtggt
     2281 cgcagatgga tcactatttt ctttactagg agacgtacat cctacaagga ataaagagac
     2341 caacaataaa ataaaaccta actttttcat ataaaatacc tcattatttt tttaatacca
     2401 gtatagcata atcctgaatt tttgaatccg taaaaccttg tct
//
LOCUS       DAHHPA010000184         2398 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.192, whole genome shotgun sequence.
ACCESSION   DAHHPA010000184 DAHHPA010000000
VERSION     DAHHPA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.192"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            207..2069
                     /locus_tag="NBH55_002820"
     CDS             207..2069
                     /locus_tag="NBH55_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD1510741.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="NBH55_002821"
     CDS             2069..>2398
                     /locus_tag="NBH55_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCD1510742.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAHHPA010000185         2385 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.193, whole genome shotgun sequence.
ACCESSION   DAHHPA010000185 DAHHPA010000000
VERSION     DAHHPA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.193"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(365..1657)
                     /locus_tag="NBH55_002822"
     CDS             complement(365..1657)
                     /locus_tag="NBH55_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCD1510743.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="NBH55_002823"
     CDS             complement(1931..2191)
                     /locus_tag="NBH55_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510744.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          805 a          444 c          362 g          774 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
     2341 gtaaaatatt ttgtgtaaat gaaaaaatcc atacaaaaaa ggaag
//
LOCUS       DAHHPA010000186         2324 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.194, whole genome shotgun sequence.
ACCESSION   DAHHPA010000186 DAHHPA010000000
VERSION     DAHHPA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.194"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>2324
                     /gene="esp"
                     /locus_tag="NBH55_002824"
     CDS             <1..>2324
                     /gene="esp"
                     /locus_tag="NBH55_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCD1510745.1"
                     /translation="VSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTTE
                     LVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTGD
                     TLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFVR
                     INDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDNL
                     KTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVSA
                     KYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVPY
                     VAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFAN
                     ASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQNT
                     YGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGTG
                     HKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSPG
                     FDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSNV
                     TDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDKP
                     DVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQDG
                     SVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          844 a          370 c          475 g          635 t
ORIGIN      
        1 gttagcaaga ataataagag agtatttcta gaaaaaacaa agaaacgagt attgaagtac
       61 agtatcaaaa aattaagtgt tggggttgca tctgttttga ttggtgttgg tcttgtcttg
      121 ggaacaactg aactagttaa agcacaagat gaaatttctc caagcagccc acttgaaaca
      181 gcgataagct ccgtacaagt aggtgacaaa gtagcttcgg ggaatacttt tcaggagaat
      241 cctggttata cgaaaaatta taacttttcg gatttacaat ttagtcctca agaactaact
      301 ggagacacac taaaaggcaa tacaattgga tttgaagttt acggaaaaca taatattgca
      361 gcatcaacta aaaactggga aatccgtctt caattagatg aacgattagc taaatatgta
      421 gaaaaaattc aggtcgatcc aaagaaagga attggatctt ctagacggac ctttgtaaga
      481 attaatgatt cgctgggtag acctacaaat atttggaagg tcaattatat tcgtgcaagt
      541 gatggattat ttgctggggc agaaacaacc gatacgcaaa ccgctcctaa tggggtaatt
      601 acgtttgaaa agagcctaga tgaaattttt aaagagatag gcatagacaa tctcaagacc
      661 gatcgtttaa tgtatcgaat ctacttggta agtcatcaag acgatgataa aattgtaccg
      721 ggtattgata gtacgggcta ctttttaaca gattcagatg atttctataa tagcttagat
      781 gtatctgaaa ataatcctga ccagtttaaa catggatcag taagtgcaaa atatgagaaa
      841 cctaatactc aaacaaaaga tggttcagga tccacaggtg ctaatggagc tataatctta
      901 gatcataagt taacgaaaaa ttataatttc tcatattcat cttccgcaaa gggcacgcct
      961 tggtatgcta attacaaaat tgatgaacga ttagttccat atgtagcggg tatacagatg
     1021 cacatggttc aggctgacaa agtaacatat gatgtttcgt ttgaatccgg aacaaaagta
     1081 gctgatttag caatcgagcg acgggaaggt catgaaaatt atggcatagg ttcaataact
     1141 aataatgatt taactaaact tatcgatttt gctaatgcaa gtccacgtcc agtcgttatt
     1201 cgatatgttt tacaattgac taagccatta gatgaaatct tagaagatat gaaagcaaca
     1261 gcacaagttg aagaaaataa accatttggt gaagatttca tctttgattc ttggttgtcg
     1321 gatacgaata aaaaattaat ccagaacact tatggaacag gttattatta tttgcaagat
     1381 attgatggtg atggaaaccc tgacgataaa gaagagagcg gagacacgaa tccatatatc
     1441 gggaaacctg aattagaaga agtatatgat gttgacacaa cagttaaggg gaaagtattc
     1501 atccacgagt tagcgggaac aggtcacaaa gcccaacttg ttgataaaga aggtactgta
     1561 ttagcagaaa aaactatcgc tccaaatgaa aaagatgggg ctccaatttc agatactgta
     1621 gaatttgaat ttacgggtgt agattcaagt aaattaatcg cgaaagatga attaaaaatc
     1681 caaatcgttt ctccaggttt tgataaacca gaagaaggtt caaccgttat taaggaatca
     1741 ccaaaagcgg ttgataaaca aaccgtggta gttggattta aaccagatgc taaagaatca
     1801 attcggaata ataaaaactt acctgaagat gcagagtatt catggaaaac agagcctgat
     1861 acttctaacg ttactgatag tacgaaaggt attgtaactg ttaagatcgg aaatcgaact
     1921 ttcgacgtgg atgtagagtt tgctgtaaaa gcttctcaag ctatggaaaa tgatgcaaca
     1981 tacgtaccta taacaacaac tccagaaacg acagttcaaa gtggaaaacc tacatttgat
     2041 aaaccagatg ttcctttagc taaagatgcc ttttcaattt cagatgtcta taataaggac
     2101 ttcggtaatg caagtgttga tgcaaacact ggtattgtta cattcactcc agctaaaggt
     2161 gtaggagaat cggagccgat tactggaaca attcctatta aaattgttta ccaagatggt
     2221 tctgtaggca cgaccgattt agcagtaact gtaagtaaag atatttatga aaatccagga
     2281 gaaaacattc ctgcaggcta ccacaaagta accttcaccg cagg
//
LOCUS       DAHHPA010000187         2308 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.195, whole genome shotgun sequence.
ACCESSION   DAHHPA010000187 DAHHPA010000000
VERSION     DAHHPA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.195"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="NBH55_002825"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="NBH55_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCD1510746.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          764 a          330 c          371 g          843 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaata
//
LOCUS       DAHHPA010000188         2257 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.196, whole genome shotgun sequence.
ACCESSION   DAHHPA010000188 DAHHPA010000000
VERSION     DAHHPA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.196"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            93..1706
                     /locus_tag="NBH55_002826"
     CDS             93..1706
                     /locus_tag="NBH55_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCD1510747.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          708 a          401 c          444 g          704 t
ORIGIN      
        1 tctattcaaa ataaacaata attttccggc acaattattt gctgtttttt accataacat
       61 agtacgatca acgtagaaaa ggagatgttt ttatgaaagt tgttgtcatt ggcggtgtag
      121 ctggcggtcc ttcatttgcc actcgtttcc gtcgattgaa tgaagcgcac gaaatcatta
      181 tctatgaacg cggagaaaat atttcttatg caagttgtgc tttgccttat tatttaggag
      241 gtgtgatcac tgaccgtgac tcgctgatcg aacgtacacc agaaatattg aaaacaaaaa
      301 acaacatcga tgtctttact aaacacgaag taacagcaat cgatccttct actaagcgat
      361 taacagttaa agacctatcc acaaatgaag aaataaaaac agattacgat aagttgatca
      421 tctcttctgg tgctagacca gattatccgg atattcccgg agtttttgaa gcagaaaacg
      481 gctttgtcct ccgtagtgtg acggatgcgg atcgaatcaa atcgttcctt gaagaaaaaa
      541 atccacaaca tgtcgtcatt cttggtgcag gtgttatggg tctggaatta gctgagaatt
      601 tcaagcatcg cggcttagac gtgactttaa tcgatcaatt gccacaagtc gctttccctt
      661 atgatccaga aattgctaat ttagtttatg acaaattgct gaaagaagga ttagccgttc
      721 atttagaaac aagagttact gagatccgtg ataaaggtcg agaaatagta ttatcagatg
      781 gctccgtcct ttctgctgat atgctaattt ttgctgttgg tgtttctccg aagaatgaag
      841 tggtgaaagc aaccggcata caattatcag atacaggtca gatcattgtt gatgaccagt
      901 tacaaaccaa tctgccggac atctatgcga ttggcgatat tatcgaaacg actagtgtag
      961 tgactggtca gccgatccaa agtatgcttt ccagtgcggc caatcgtcaa ggacatatgc
     1021 tggcagatat tttaaatggt acgcctatgc gctatcgcgg atatattggt gcaggtgtcg
     1081 caaaaatctt tgatcataca gcaagttatg cgggaatgac agaacatgaa ttaaaagcat
     1141 caggcataac aaattataaa actgttttta tcactccctt tgaccatgcc tatttctatc
     1201 cagaagctac aagattaaat ctaaagctga tttttgatgc agatagcggt cgtattttag
     1261 gtggacaagc atttggagaa aaaggtgtcg ataaacggat gggagaactt tctgtagcaa
     1321 tcaccggaaa cttgacagtc tttgatttgc ctgatttgga gttgccttac tctccaccgt
     1381 attctactac ccgtgacccg ttgaatatag ctggttatgt cgcaatcaat caaatgacga
     1441 atatcgtaga aacgatcaaa gcaagtgata tacccgaaaa cgatttgaaa gaagcgttct
     1501 ttttagatat acgtgaacct aataaagcac cttctggcag tatctcggca acaaaaaata
     1561 ttccaatgaa tgagttacga gatcgaatca atgaaatccc aaaagataaa aaagtttaca
     1621 ttactttcag aagaggattg aatacttata cttctgcccg aatcttggca ggtttgggta
     1681 tcaaagcgat tttgattgaa gaataaaaaa acgaccgcgg tgggtagccg cggtctagga
     1741 gttattttta cttgaggagt aaaaatgaaa aagtatatag ggttgttggt tggtatgtat
     1801 atagaataga agataaatgt gaagaaattg tgcattattt cttcacattt attaggttat
     1861 agtttaagaa agtttaaaca ttgcgcaatt taattatttt agctgtttct tttcataaaa
     1921 aaacatgttg tgactctgag acaaatcata gtccatagtg atgtctctag agtaactacg
     1981 cctaatatta cgaggcatca tattaatcta tcagcactag tacctaaact agcatccctt
     2041 tcgaatattt tttcttatat aataaagttt gctttcactc ctctaatctc accaaatcga
     2101 ctggcttgcg ttgtgtgata ctaggaactg attggtttag aacgactttt ctattgtaat
     2161 catatttttc gccatcctcg ttgtcttcta atccttcaat gaatgatccc taatgtgtgt
     2221 attaaataat ttaatttttt tgttcctatt tatttaa
//
LOCUS       DAHHPA010000189         2187 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.197, whole genome shotgun sequence.
ACCESSION   DAHHPA010000189 DAHHPA010000000
VERSION     DAHHPA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.197"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..887
                     /locus_tag="NBH55_002827"
     CDS             <1..887
                     /locus_tag="NBH55_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCD1510748.1"
                     /translation="IPSYLNNREANNIKKKMLIPLESEITGLLNKLTISNVDSTREKI
                     IRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVKVNDEKEQQKAIKEQMIEEE
                     KVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVEKELYADKIKELEEKLRELE
                     TVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRRLEPMDRIKELSSASVPFEF
                     DVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKVSLDEIEKVVLENYNGTVSF
                     TKLAQAEQYRRSLELTDV"
     gene            953..>2187
                     /locus_tag="NBH55_002828"
     CDS             953..>2187
                     /locus_tag="NBH55_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1510749.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSK"
BASE COUNT          949 a          269 c          359 g          610 t
ORIGIN      
        1 atattccttc ttatttgaat aatagagaag ctaataatat taaaaagaaa atgcttatac
       61 cattagaatc agaaataact ggacttctca ataaacttac tatttctaac gtagattcaa
      121 ctagagaaaa aattataaga acttttgata aaataaacaa gctttttaaa aacgatgaag
      181 cagaattcaa aaaagaatta cttgaattaa aacttcaaga attagaactc aactattcat
      241 acatagttaa agttaacgat gaaaaagaac agcaaaaagc aataaaagaa caaatgattg
      301 aagaagaaaa ggtaagacgt gaaatagaac gtgaaaagaa acgcattgat aaagaagaac
      361 gacagtttaa ctcagagata actaagttaa tggcatacat gcaaaaagct agtgctgatg
      421 ttgaaaaaga actctatgct gataaaatta aagaactgga agagaaatta agagaactag
      481 aaactgtcaa agaagatgta ttgcaaagag aacttaatac tagagctggc tatgtttatg
      541 tcatcagtaa tatcggatct ttcggtgaag atgtctataa aataggaatg actagacggt
      601 tagaacctat ggatagaatt aaggaactta gtagcgcttc tgtacctttt gaatttgatg
      661 ttcatgcaat gatattttca gaagatgcac caacattaga aaatcaacta cattcgcatt
      721 ttagaaaaaa cgaagtcaat aaagttaatc aacgaaaaga attctttaaa gtatctcttg
      781 atgaaattga aaaagttgtt ttagaaaatt ataacggaac tgtatctttt actaaactag
      841 ctcaagctga acaatacaga agatcattag aattaacaga tgtgtagtaa aaagattagc
      901 cttcgggctt ttcttttaca acgataagaa catacattcg aaaggagata acatgaaacg
      961 agtagcatta tatatgagag tatcaactga acaacaagcc aaacatggag atagtcttag
     1021 agaacaaaaa gaaaccctct atgaatatat cgaacaacac aaggatttga aagtagttaa
     1081 tgaatatgta gacggtggta tttctggtca aaaaataaat cgtgatgaat ttcaaaaact
     1141 attacaagat gtaaaagaaa ataaaataga tctaatttta tttacaaaat tagatagatg
     1201 gtttagaaat ctacgacatt atttaaacac tcaagaaatt ttagaaaaac acaatgtttc
     1261 ttggaatgct gtttctcaac aatattatga tacaactaca gcgtatggca gaactttcat
     1321 tgcacaagta atgagttttg ctgaattgga agcccaaatt gattcagaac gcatcaaagc
     1381 tgttatggca aataaaattg cacaaggtga agtagtaagc gggaaaactc cgttaggcta
     1441 ctctatagaa aataagaaat tagtaataaa tgatgatgct cctatcgtga tagatatatt
     1501 caactacttt ctttctagtg gtagtttaag aaaaactgtt tattatttag gctctcagta
     1561 tggaatcgtt agagattatc aaagtgttaa aaatatgttg acgaacaaaa aatacattgg
     1621 tgaattaaga aataataaaa attactgtcc tcctattatt gataaaaaac ttttttatgc
     1681 tgttcaaaaa gccttaccta aaaatttaaa aacaaatgca aaacgtgatt acatttttaa
     1741 aggtttatta aaatgttctg actgtcaggg atcagtagct ggtcaaacta ttaaagcaag
     1801 atataagaaa aaagacggta cagaatctat atacgaaagg acttgttata gatgtgtaaa
     1861 acgaagaaat aataaattac gttgcacaaa taagcgcgct ttttacgaaa aaaatttaga
     1921 acgttatatt tttgaagcta ctaaacaaaa atttgaacaa atacaaataa actattcaaa
     1981 aaaacaacca aagattatta aaaagaaaaa tagtaagaaa aatattgaga ataaattaga
     2041 tagattgaag aaagcctact tgaatgaagt tatagactta gaggaataca aaaaagatcg
     2101 agaagcttta atgaaagaat taaatgaaat tgaagtagag ccagctaaaa tagatataaa
     2161 aaatgtcgaa tttattctat caaaaga
//
LOCUS       DAHHPA010000190         2141 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.198, whole genome shotgun sequence.
ACCESSION   DAHHPA010000190 DAHHPA010000000
VERSION     DAHHPA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.198"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(119..709)
                     /locus_tag="NBH55_002829"
     CDS             complement(119..709)
                     /locus_tag="NBH55_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCD1510750.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1232..1393
                     /locus_tag="NBH55_002830"
     CDS             1232..1393
                     /locus_tag="NBH55_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCD1510751.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1412..1906
                     /locus_tag="NBH55_002831"
     CDS             1412..1906
                     /locus_tag="NBH55_002831"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCD1510752.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1899..2060
                     /locus_tag="NBH55_002832"
     CDS             1899..2060
                     /locus_tag="NBH55_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510753.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          769 a          333 c          364 g          675 t
ORIGIN      
        1 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
       61 ccatcagtaa aattacttgt catactattg ataaggagta tttaattgaa gagaatgatt
      121 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
      181 tctttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
      241 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
      301 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
      361 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
      421 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
      481 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
      541 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
      601 tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta atataagcac
      661 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
      721 cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt aaatttggat
      781 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca gtcaacttaa
      841 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
      901 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      961 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     1021 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
     1081 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
     1141 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
     1201 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
     1261 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
     1321 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
     1381 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
     1441 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
     1501 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
     1561 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
     1621 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
     1681 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
     1741 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
     1801 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1861 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1921 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1981 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     2041 tagttgggtt ggataaataa attttttaaa ccatatcctt gtaaagttga gttttttatt
     2101 ttaatgaatt aagactatta aatagataat tattgtaact t
//
LOCUS       DAHHPA010000191         2133 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.199, whole genome shotgun sequence.
ACCESSION   DAHHPA010000191 DAHHPA010000000
VERSION     DAHHPA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.199"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(96..1022)
                     /gene="cysK"
                     /locus_tag="NBH55_002833"
     CDS             complement(96..1022)
                     /gene="cysK"
                     /locus_tag="NBH55_002833"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCD1510754.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1162..2049)
                     /locus_tag="NBH55_002834"
     CDS             complement(1162..2049)
                     /locus_tag="NBH55_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCD1510755.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          590 a          476 c          356 g          711 t
ORIGIN      
        1 ctattcaaat aatttttctc atcataaatt tttgtgtgtt cttttcatct ttaaaagttt
       61 tttacataaa cctatctgga gaaaaaccaa gtgccttata tctcttggta aagggctgta
      121 gataaataac gttcaccatt gtcgggaacc acagctaata ccttcttgcc ttttcctaat
      181 tcttttgcga cttttagcgc ggcagcaatt gcagcgccag atgagatgcc tactaaaatc
      241 ccttcttttc gcccaacttc tctagctgtt tccatcgctt cgtctccact aatagaaagg
      301 actttatcat acacagaagt atccagtgtt ttaggaacaa aacctgtacc gattccttgg
      361 attttgtgag gtcccgcttc tttcccttct aaaatagcag attctgctgg ttctaccccg
      421 attaattcga ttttcggata cacacgtttt aattctcggc cagcgccagt gatcgttcct
      481 cctgtaccga tacctgcaac aaacgcgtcc aagccattta ctccaaatgc ttgatgtatc
      541 tcaggaccag tcgtcttctc atgcacaaga ggatttgctt cattttcaaa ttgtaatggt
      601 aaaaagtagc cgttttcttt tgccaaacgt tctgcttctc tgattgatcc agaaatacca
      661 tccgcaccag gagtcaaaat caactctgcg ccgtaccctt tcatcaataa acgtctttcg
      721 atactcatcg tctcaggcat cacgatgatt actttatatc cttttgcaac tcctaccatc
      781 gacaatccga ttcctgtatt tccagaagta ggttcaatga tagtatcacc tggtttcagt
      841 agaccatcgt gttctgcttt ttctatcata cttaaagcaa tacgatcttt tacacttcct
      901 cctggattaa aaaactcaag ctttacaaaa acatctgctg aatcttctgg tacgatttta
      961 tttaatttga cgattggcgt tttgccaatc aattcggtga ctgagttata gatagctgtc
     1021 atcttcaatt cctccttgtc tttgctcttc ttacttatca taatacaatc tgttcattta
     1081 tggcgagtga ttcgtctttg actagcaaaa aaaagacaaa gatataccat atatggtaat
     1141 ttctttgcct ttttatctaa atcaaacgtt tagttcttga ccaactaata aagaataact
     1201 ggacatagac atgccatttt tcgccattag atcatcaaca gacatgccaa atttttgtgc
     1261 aatatcccat agcgtatcac ctgacacgac tgtatatgtc tgaccattat ttgtatttac
     1321 atctgttgtt gaagatgatg acgttgaagt cgtcgtagta gtcgttgttg ttgtacttgt
     1381 ggttgttgta gccgaacctg ttgatggcgt atcaaaacgg gttaaattat aagtattaat
     1441 caatgagttc aatttggccc cataatttgg atcggtagca taacgacctg tcaagtacgc
     1501 tgttgcatca taaaaacttg ttgtattgct tttccacacg ccagcataat gataatcgcc
     1561 cgtggaaaaa ctggttgaac gaagaacatt cgcatggtct tggaaggatt cccaataaga
     1621 agaatatttt cggaatggct cattcatcgt tacccattgt ccgtttacat attctttggt
     1681 tgccatataa acgacctgtc cgttatagct tcctttcaca ccgaatagat tataatttgg
     1741 cgcactagct aatccggaag tgccatatgc actttccaac aacgcttgag cgatcatgac
     1801 tgaagcatac aagtcattac ttgcagcaac ttctgacgca gaccagccga tttgatcgat
     1861 aaacgcttga ggatcagcga ctgtttgaga tccttgttca tctgcatgaa catgttgcgg
     1921 taccgctgta accatcatcg gcccaactaa aagactcgtt ccaattaaag gagcaatcgc
     1981 ttttttattg atcaccattt ttcttttagg tctatggtgc cgggcactgc gggtcttcag
     2041 ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc tcttcttaaa
     2101 acaaacttaa ttaattaaat tatactttta att
//
LOCUS       DAHHPA010000192         2104 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.200, whole genome shotgun sequence.
ACCESSION   DAHHPA010000192 DAHHPA010000000
VERSION     DAHHPA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.200"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            169..1539
                     /locus_tag="NBH55_002835"
     CDS             169..1539
                     /locus_tag="NBH55_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HCD1510756.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="NBH55_002836"
     CDS             1560..1844
                     /locus_tag="NBH55_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510757.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DAHHPA010000193         2032 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.201, whole genome shotgun sequence.
ACCESSION   DAHHPA010000193 DAHHPA010000000
VERSION     DAHHPA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.201"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            312..719
                     /locus_tag="NBH55_002837"
     CDS             312..719
                     /locus_tag="NBH55_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510758.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            733..1134
                     /locus_tag="NBH55_002838"
     CDS             733..1134
                     /locus_tag="NBH55_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510759.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1136..1507
                     /locus_tag="NBH55_002839"
     CDS             1136..1507
                     /locus_tag="NBH55_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510760.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1543..1845)
                     /locus_tag="NBH55_002840"
     CDS             complement(1543..1845)
                     /locus_tag="NBH55_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510761.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          765 a          273 c          338 g          656 t
ORIGIN      
        1 taacttttgg ggtgcacatc atttttgagg ggaagtacat atagttgttg atatagttgc
       61 taatattgtt agctaatttg ttagtaaaac tgtatacgaa tttagatagt aaaaaaacag
      121 tttgttcctt atatagaggc acttttacta ctgattaaat gtcagatatg cttacaaata
      181 tacttataaa tatgcttaca aaattagcta atatttccgt tgacaaagct ttgttactat
      241 tgttaaacta aattgactag caaaagtgaa acataaattt attaaataaa tgtaaaaatt
      301 aggagaaatc aatgtcttat agagtacaat taataatttc agaagatgta gaaagtcagc
      361 aatttggcac taatgttatt aataaagtaa ttaatccagc tctttctatt aatgcgcctt
      421 taattcctac tgctttatca ttttctgtaa cagctgtggt gtctgagata gaggatacag
      481 aaaagataaa aatagtagaa attgaagttt taaacaagaa tgaaaaacaa attttttcta
      541 cgggtgaagt atcggtaagc ttgcctcctc aagttaacga tataaacttt aatataaacg
      601 ccagaaatgt cttggttgaa gaagcaggag agcattatgc tgttgttaaa tttaatggaa
      661 ctgagattgg aaggcaaatc tttgatatca aggtcaacaa accagtggaa aaaaattaag
      721 gagacgatac ggatgcttga tatagtacca tcaaatacac atcggagtgg tagcttaata
      781 aattttgctg cgatttttgc ttgcgcatcg gtaattctag catctccagt tgttgtggat
      841 gcaagttcta ctccgaaaac taaaaatgat aatcaacttg taattactac aaaaaatgag
      901 attaacacaa tttcaagtaa tgatggcaaa atttttgatg tgataaatac ggttttaaaa
      961 agaaaagctg aaatcaatat aaaatatgat gaagatttaa acttgtattt ctttgttatc
     1021 aaaacaacat ctgaattgtt ttcctcagat tacgatgttt tggatactct cgataatgta
     1081 ttatctgact ataaatatat ggggaaatct gtagtagcaa ctttggggga ataaaatgtt
     1141 tgactttaat gaatatttat ctatttccaa acaaattgaa tcagatgata aatataattc
     1201 taaagaatct tatagaagaa ctgctgtttc aagagcatac tacagtgctt ttaaaaaatc
     1261 tgatgagtat ttgaaagaaa attatgatat tatttataat ggaagtagcg gaaaaggaag
     1321 ccaccaaacc gtttggaatt tgttttcaac agttaaggaa ttgaatacct taggaataca
     1381 taatagtggg tatagaatgt tagagaaaag aaaatgtgct gactatattc ctaatgaaac
     1441 cattacgaaa acagatatgg cgctgatgaa tcgagaagca gaaaaaataa taaacaaact
     1501 cacctaatga atttcaagcg agtttgtttg ttattttttt atttaatctt tgtatctttc
     1561 tggatcaacg aaagtatact ttatatagtc ataacgccga tgatcgctac gtgcgtccgg
     1621 cacgtcagtc acgatatcaa acaaaaagta tacacccttc ttcattctag ttttcgcagc
     1681 aggaattttg aaatagttct tattagaata gtagagattg attaataagc tatcttcgat
     1741 tgctaaaaag aaaacttctg aattccatac tttataaaaa tctttgataa atctattcga
     1801 agggtcaaat ttaaaccata attgtgtttt tccttccatc agcataaaag ttcacctcaa
     1861 aaagagtata cgaacgaatg ttctttttgt gaaggcggaa tttaatatac tgaataatag
     1921 aaaacaagta cttgtgccaa attatgtgcc aaaaaaattc gaattcaaca aaatgtaaac
     1981 aaatagaaaa ccttctatat taagatttcc tgcaataaca aacacaacag aa
//
LOCUS       DAHHPA010000194         2015 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.202, whole genome shotgun sequence.
ACCESSION   DAHHPA010000194 DAHHPA010000000
VERSION     DAHHPA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.202"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            599..1117
                     /locus_tag="NBH55_002841"
     CDS             599..1117
                     /locus_tag="NBH55_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCD1510762.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          696 a          293 c          386 g          640 t
ORIGIN      
        1 cacttattta cccaagctga aaagcctgat attacttctt ttcaggcagt gctgctaata
       61 ttcaattaca cttttttgta taaatttagt tgttttaaat ttaattgtct gctatgcttg
      121 tgatgaattg attttcgttc tgcacttttg gacgataaat ttggacaatt gaaaatacaa
      181 aaaaaaagaa aggccctgtg agactcggaa ggctaccaac cttcgtcgac gtccacaggt
      241 ggcattaaga tcactccctt aatgcgttta cctttcagtg ttacggtatt acccgtaacg
      301 gatttagtat aacagatatt caaaagtgat accacttttt ttggctgtgt taaggttaaa
      361 aatacgtgat attacgatga atctatgcct aagcataatg ttataaaaat gtccgtgtgg
      421 ctctttgccg tatgacgaac gctgaaatta ggagacgttg ggatatttta taaatcattc
      481 acatatgagt tagacagtca atgcatatca gatatgcatt gtagaacgga ttaaaatatc
      541 gtggatgaaa ttatagatga attaaaggag acaattagga atttatggag gtaatataat
      601 gagaacatat aaaaataaag aagaattaaa aaaagaaata aataggactt atactaaata
      661 tattttagaa tttgaaacta ttcctgaaga attgaaggat aaaagagttg aggaagtgga
      721 tagaacacct gctgaaaatt tagcatatca agttggttgg acgaatttaa ttctaaaatg
      781 ggaaaatgat gaaagaaatg gattatctgt taaaacgcct tctgatcaat ttaaatggaa
      841 tcagttagga gagttatata agtggtttac aaatacctat gcacatctac cattgaaaga
      901 attagaggaa attctcaatc gtaatattga tgatattaac atgatgattg attcaatgag
      961 tgatgaagat ctattcactg cacacaaaag aaaatgggca gacgaggcaa ccaaaacggc
     1021 tgtatgggaa gtatataaat ttattcatgt aaatacagtt gctccatttg ggacttttag
     1081 aacaaagata agaaaatgga agagattagc tttgtaaatt cccgtttact gctaagcaat
     1141 tgtggaaagg agacctcttg tatgttaaca gtaagaacat acgactacat aagttctttg
     1201 ctaactgccg gtttttattg ggtgaatgca agtgcactcc cctagaatgc cgtagagggt
     1261 gcatcgttag attgtatcaa ttttggttgg ccaacagata aaccatgact cacttcgata
     1321 aaatgagatt atggtgccat atccgctaca aagggactta ggagactagc aggggaaaga
     1381 tttttttatt tttgggatgt ttttcagacc gccgatagta ccgacaattc cctttaaata
     1441 tggaatctca acaacccaca tgaagggtaa ggcaagtccg gtaattatat cttctgactt
     1501 tgggttagga agaattgaaa ttgttaagaa cgattcaacg cttatggttt ataatttgga
     1561 gatagccttt gttaatatgc taaagccaac atacgataca gattttgagc agtttattct
     1621 aaccatcaaa gggtatgtgg cctatcgcct atcctattac tgatactgaa gagatcttaa
     1681 ataaattcta tacgggattt gaagatcctg aaggtgaaat cgtcatttat cagaataaat
     1741 aaatcaaata gtagaagttc aaagaatgag gggaacatta gtttttattg cttagtttaa
     1801 tcccagtacc gatttctgca agatgtaaaa ggtctttcat ttcataagtt ttcagaagca
     1861 agttcatacg cactaactca ctttcgattg agtagcaatc gtcaacaaac tttactatca
     1921 gctgttattt ttattctttc gtgttatcta aataccatca atttataagt tgtaggtgta
     1981 tttaataaaa gaacaaataa ctttagtatt tttat
//
LOCUS       DAHHPA010000195         1992 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.203, whole genome shotgun sequence.
ACCESSION   DAHHPA010000195 DAHHPA010000000
VERSION     DAHHPA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.203"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..425)
                     /locus_tag="NBH55_002842"
     CDS             complement(<1..425)
                     /locus_tag="NBH55_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCD1510763.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="NBH55_002843"
     CDS             complement(534..1565)
                     /locus_tag="NBH55_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1510764.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          613 a          328 c          346 g          705 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gc
//
LOCUS       DAHHPA010000196         1945 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.204, whole genome shotgun sequence.
ACCESSION   DAHHPA010000196 DAHHPA010000000
VERSION     DAHHPA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.204"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(662..952)
                     /locus_tag="NBH55_002844"
     CDS             complement(662..952)
                     /locus_tag="NBH55_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510765.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1160..1852)
                     /locus_tag="NBH55_002845"
     CDS             complement(1160..1852)
                     /locus_tag="NBH55_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCD1510766.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          533 a          369 c          299 g          744 t
ORIGIN      
        1 tacttaaaga taacttaaaa gttatctttt aaatacttaa ttgttaaata ccttatttga
       61 tgcaaaagaa atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc
      121 ttagttaagg acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca
      181 tacaatcata ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc
      241 caaatgttag gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca
      301 atttactttt acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa
      361 cgtttaaaat cctacaattc cactacctga gactagtaat gactagtctc tttttatggc
      421 ctctgatccc cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta
      481 agcaatatcc ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca
      541 tgtagttata tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat
      601 cgtctgcatc catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt
      661 tctagtttat tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt
      721 ctaccacaat tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat
      781 tattataata acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca
      841 aactatggga agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg
      901 cactaacttg caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca
      961 cctcaatacc attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt
     1021 tttgactttt tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag
     1081 gacatgtact aaaaaaaaga ggcagcagta tagattactg ttgcttcttt actaatttag
     1141 gtttacttgt taagaaagat tatttacgca aatgttcttc aacaatttgc tgagatttat
     1201 cagctgtatc ttccacttca tcttttgctt tttctaactt ttgaatatat tgtttcaagc
     1261 ttgagttttc agcagttgct tcgtctaatt tcttttgtgt atctgttaac tgagtttcta
     1321 catttgtttt ttcttgtgtc agtgttgcga tttgttgatt caaaccattt acagtatttt
     1381 gtaattctga aagttttgct tgtgcttctt gtagtcttgc tgtccaatct gcattcagat
     1441 gattgatttc atcatttttt tggttgatct catctagttt gacattgatt tcattttgtt
     1501 tgctttgtag ttgtgcatcc ttgctgacta attgactttc taagttttgt ttttctgctg
     1561 ccgattgatt tttttggttt tctaattcat ttacttttgt ttggtattct tggacatctg
     1621 cctttgaatc ttgcaatgtc tgttcttttt gtgctagtcg agcggtaaga tctgtaagcg
     1681 cttgctcttt atctgtcaat gtatgatcaa gctgtgtcag attctcattg attgtatcaa
     1741 tatctgtctc tccaccccac aaaataattt catttgcgta ccgcgtgcct gtgtagccaa
     1801 ttagacatag aaatgcaacg acaatcccac cagtaataaa tttctttttc atttgcatag
     1861 ctccttaatc caatactttt acatgctaaa agtaagtgat ctcaaacaca atgtcaagcg
     1921 tttttaaatt acatgtgtaa tttta
//
LOCUS       DAHHPA010000197         1921 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.205, whole genome shotgun sequence.
ACCESSION   DAHHPA010000197 DAHHPA010000000
VERSION     DAHHPA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.205"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..195)
                     /locus_tag="NBH55_002846"
     CDS             complement(<1..195)
                     /locus_tag="NBH55_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1510767.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
     gene            869..1681
                     /locus_tag="NBH55_002847"
     CDS             869..1681
                     /locus_tag="NBH55_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510768.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
BASE COUNT          622 a          284 c          317 g          698 t
ORIGIN      
        1 ataatagcca tttcgttgac tttgtcggtt ttctgttcgt tcatattctt ttgcttgaat
       61 atattctgtt cgttgatttt ccattagttg attaaatacc gttgttaaaa tatttttaga
      121 aacgtcatcc tttacagaat attcaataat gctttgaatc tcttcgcttt tcagtgtaaa
      181 atgtacttgg gtcatgtaaa agtcctcctg ggtatgtttt tgtcgttaaa aacattgtac
      241 cgtaaaagga ctgttacatg gcctttttac ttttacacaa ttataaggac tttatcgaaa
      301 aaaagcagtt atttgttaaa atcattgata ttattaataa taagtatttt ttgaaagggg
      361 aaatataaaa ggtttttgaa aataaataaa aaatagtatt acttgttcta ttgctagtta
      421 ttgctagtct ttattctttt ttgtttttta gggatgtaca aaagatctca accacaggag
      481 acattgcttt tcacttttct agaataaaat gcttgtcctc agtattttct ggtccaataa
      541 attttaccac atttaatagc tatggcggag gcgtgaattt ttttatccat atctaacatc
      601 tttccagccg taatatttta ctggacaaca aacaatttga ttctaagtta tatacttact
      661 atacgttggg atactgaata tctgtacaat tctagtgagc tatcaatatg gacaaaagaa
      721 tagatgcagc cttcttattc agctgtttgt atagctttta tggattgata tatattagcg
      781 tactgcaatt tcagaatcaa ttgcgttgac gattgtacca gttgtcttgt attacacata
      841 tactcttctt tatgaaagaa aaaactgcgt gattctttta gctgtcagta tgagtttgct
      901 catctatacc catattctct ccacctttat gagtgtgatt gttattggct tcttgtttat
      961 cggctctctt ttctctaagc ggaaagagag aaaagaaatg tcgaatctga tatcaaatat
     1021 gataaaagct gttggttgga ccattctttt aacagcttac gtttggtatc cgatgttaca
     1081 acaagtccta catcaaaaaa ttaatcgtcc ttttcgcaca aaacttcaag aaagggcatt
     1141 aaacatttct gatagtttaa tcggtgcagt aaataatgat ttagttacat ttacaatggg
     1201 aacaattggt gtggtttcat ttattcttcc acttttcttt aaaaaaaaat ttgccaataa
     1261 agaaaagatt attaattttt gtgcagtgac cacttggttc ttatcaacga atctttttcc
     1321 atgggaattt ttacaaaaaa cacctattca aatcattcag tttccttgga gaatcttggg
     1381 ttttcaagtg ctttttggtt cattgatctt aattatagtg tttttaaagt ggaaaacaag
     1441 caacaaaaaa agtatgtgta gccttgtagg aatagtcctt cttattttta cagtgactgt
     1501 agcaacagaa gcgaattatt cgcagaaaat tcaatcgtat aaaggtcgtt taattatgac
     1561 caaaaaagat gttacacttt atacaacctc gagaactgga ggtctttatg actatgcacc
     1621 attagatgca ttgaaatata aagatcattt aaagaaacat gaagtaaaag tctatgattg
     1681 atgggagaat gcatctcata aagcacataa ttctaaaata atctatacag taggagatgc
     1741 gaatgagcaa aatatcacgt tacctgtatt taattactgg gaacagttat gaaagtaaat
     1801 gggaaaaaga gcactataga aaatgacaat ggtttagtgt ctattaaagg tacgggaaaa
     1861 agaactatag ttgaggtttc atctacgtat ccaactacga tgattctatc tttattaatt
     1921 a
//
LOCUS       DAHHPA010000198         1912 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.206, whole genome shotgun sequence.
ACCESSION   DAHHPA010000198 DAHHPA010000000
VERSION     DAHHPA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.206"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(1..282)
                     /locus_tag="NBH55_002848"
     CDS             complement(1..282)
                     /locus_tag="NBH55_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510769.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVRESLNKDNNVERIKNIFGYGIEEINYYIDFAEAFIPMVR"
     gene            complement(287..631)
                     /locus_tag="NBH55_002849"
     CDS             complement(287..631)
                     /locus_tag="NBH55_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1510770.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(1449..1862)
                     /locus_tag="NBH55_002850"
     CDS             complement(1449..1862)
                     /locus_tag="NBH55_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCD1510771.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT          548 a          450 c          262 g          652 t
ORIGIN      
        1 ttatctcacc atcggaatga aagcttcagc gaagtcaatg taataattaa tctcttcaat
       61 gccatatcca aaaatatttt ttattctttc aacgttatta tctttattca atgattctct
      121 cacttgattc actttgtatt tactacaaca gttatctgat aacagatccc taatacctac
      181 atagcgaacg tatatcaaac gtttaattaa tccttgtgta taagatgaat actcttcaat
      241 cttttctgta tcatactctc tgccattgtc attgatgatc atgcacttac cacctttcac
      301 ttgcgtcgaa gttagcaaag ctttctatct tcttctcttg tttatctaac gctgtaagat
      361 atcttccatg aacttcatta tgatgttcaa cacataaaca aataagatta tctaaatcta
      421 aagctaagtc aggtctatcc ttgacttcct ttatatgatg aacgttctct actctatgat
      481 acttacctag tcttctacac tcttggcatt catagtgatc tcgtttcatc gctttctctc
      541 taagcctgcg ccatttagga gactgataga acttaaccaa acgatcttct cttatcaact
      601 gtaatagcca tctatagaat tcctcggtca tgccccgtct cctttcgcaa tcttatttaa
      661 tgcttagcta ttcttttgcc atacaatgga ataacttcat tcctttcctt tcgtttatat
      721 gtatcgctct ttattggtct tctatacttt cgtactatct caccgttacc attttgcaca
      781 gtgattactt catacttctg ttctaagtat tgtggtctat acattgttgt tacctccttt
      841 gtgcaaaata aaaagaccac tcaacgagtg atctaatatg taatagcaac ttgcaccaaa
      901 gttggaattg aaccaacagc ccgccctaat aggttctacc acggacctca cctagattca
      961 gagatttgaa ctctacgaca catcaggtat ttatgtctac caagtgcaag ctgcttaagt
     1021 cactggcaat gaatcgaaca ttgcatagtt gccgaagcat tgacctagca cacatgctta
     1081 gcgtctaccc tttccgccac agtgacaata taaaaatttc tttcttagtt actactatct
     1141 tttattttgc ccatttttaa atccaatcat atagacattc agacagagcg caaaaattga
     1201 aattattaac gccatcattt ctcttcaccc acctttagtt atcgtgtgaa taattaaaaa
     1261 aacaatagac agcaacggat gatagataat aagaacaatt tagaaggagt tgaaattcac
     1321 atccttactc ttaatatttc cgctgctgtc tatcttgttt gtcgatcctg tccaaatctt
     1381 tcgacactat cataatatca ctggtaaata gctaaaaacc gccatcattc cgccaaaaaa
     1441 ccgccaaatt atttataagc aattattctt ccgtgtttat atgcttctgc aaactctatt
     1501 agagcttccg atttcatccg ttgtatactt ctttctgaat aacccacttc acggctaatt
     1561 ctgtagtttg agaagctgtc tggcacacaa aagctgtagt agagtatctg acgactaatc
     1621 agactaagag ccatcaaagc cgctaaaatc gcgtctctct ccgcttctat atccatcatc
     1681 tgaatgatcg cgtcctctgc cttattgccg tgcttcggtg ccttcggcat atccgtaata
     1741 atcggcgact taatatctat caaagagcga cctgccatcc gctccaaacg ccgaaagttc
     1801 ttcagcacat ctctcgcatt acatcttgtc tgtttgaaat ctacctctcg taacaattgc
     1861 atcaagtcaa accgctcctt tatgtgatat aataaacttg tcggatttat ta
//
LOCUS       DAHHPA010000199         1865 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.207, whole genome shotgun sequence.
ACCESSION   DAHHPA010000199 DAHHPA010000000
VERSION     DAHHPA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.207"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            76..711
                     /locus_tag="NBH55_002851"
     CDS             76..711
                     /locus_tag="NBH55_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HCD1510772.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            824..1459
                     /locus_tag="NBH55_002852"
     CDS             824..1459
                     /locus_tag="NBH55_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510773.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            1758..>1865
                     /locus_tag="NBH55_002853"
     CDS             1758..>1865
                     /locus_tag="NBH55_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HCD1510774.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKG"
BASE COUNT          737 a          281 c          312 g          535 t
ORIGIN      
        1 gaagaattcg attttaagaa aaaagaattg cttggtttat aatattcaat atcaaaagag
       61 aaagaggatc tattaatgga cggtatattt ggttttactg gtattgtttt atttattata
      121 ggtttaatca tgttaattgt aagatttatc aaaaaaacaa ataagaaaac acctatgatt
      181 ctccttatta tcgggattat ttttactacc gtaggtttct cgctttcctc atcaaacgag
      241 gcaaatgaca ataacaaaca ggaaagctca tcatctgata ctcaaactac tacatctaac
      301 agcagcaata aaagcctaac tcaatttgaa gaatttttag agacaaataa tcatgactgg
      361 ggtgctttct tagatagtta ttattctatt actccagcta ctgagcaaaa tacagctttt
      421 actaaatata ttagtggtaa aacatatact tttgaaggaa ctgttatcga atcaatgact
      481 actagaatag ccattatagc agacaaagag tacgatgata aaagttggaa tgatatttct
      541 gcaacaccta aagtttcata tgtaattttt gcgaaggact taaacaatgc tgataccttt
      601 acaaagggcg ataaagtaac ttttacagga gaaattagtt caagaggatc gaacatagaa
      661 aaatcctatg ctcaatggga tatgattaat agtaaagtaa gtaaaaaata aatgaaccac
      721 gccccactaa gagttagatt gtgcacaata ataaaaccta tacaaaagag tgccatcagg
      781 agtcacaatg cttgtaaagg aaatgacaaa ataggaggta actatggata atagcaatga
      841 aaaactaacc aaaaaagaat atcttgaatt aatagttgaa ggcggtattg gagcaattcc
      901 ttatattggt ggcgttgtgc agtcacttta ttttgggtct aaaaacgaga agcgtttcaa
      961 acgcatcgag aaattctaca aaaatttaaa cgaatcatta gaaaacattc gcgatcaaat
     1021 tccagaagat gttttcaact tagagaacaa ggaccaactc attggaatat tcgaagggat
     1081 aaatgacgag gtagaaaaat caaaggcaca aaataaaata gatatgtata aaaatcttta
     1141 taagaattgt ttacttcgaa ttaacaacgc ttcatgggat aacgaggagt attatcttct
     1201 tgtactgaat caattaacat cgattgagat tcaattgtta gcatgcttaa tgagtcgtgg
     1261 aaacgaaaat ttcactggaa atatcagcat gtctggctat tcacaagagc taatagatgg
     1321 tagcttaaat agattatctg attttggtct gttagaaaaa acgattagct ccatagtatt
     1381 aggtgatgtc ggcaaacaaa atatgggcta caaaatatct catttaggaa ttcaatttat
     1441 taactcaact cttacttaag ttttctttct aatatgacat ctagacctat cagcaatgca
     1501 acaataaaaa accagacata ccaataatca acaaataatc taaactctgg aagttcagtt
     1561 attcttttaa aaatcacttt aatcataata aacacctctg attggatgat ctaatacaca
     1621 tccaattata ccattaaaac gacaaatagc acatccttgc gccggcaagc aaaaaggatg
     1681 tgcaaaaaat aataaaaccc aaacaatggg cttctttata gttcctattg tatcagagaa
     1741 agagagctga ttcaattatg gcaagattag tcaaacgtgg aaatagttgg caatacgaaa
     1801 tttcatacaa aaaagatgac ggaaaataca cgaagataag aaaatcagga tttaagacaa
     1861 aagga
//
LOCUS       DAHHPA010000200         1856 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.208, whole genome shotgun sequence.
ACCESSION   DAHHPA010000200 DAHHPA010000000
VERSION     DAHHPA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.208"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            34..1368
                     /locus_tag="NBH55_002854"
     CDS             34..1368
                     /locus_tag="NBH55_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510775.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT          646 a          263 c          314 g          633 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttact ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttatttt aagtagttca
     1741 gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt cattttaact
     1801 tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga taattg
//
LOCUS       DAHHPA010000201         1808 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.209, whole genome shotgun sequence.
ACCESSION   DAHHPA010000201 DAHHPA010000000
VERSION     DAHHPA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.209"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..289
                     /locus_tag="NBH55_002855"
     CDS             <1..289
                     /locus_tag="NBH55_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510776.1"
                     /translation="ILQRSEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATA
                     TTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENCWIGEEKLNEIY"
     gene            276..728
                     /locus_tag="NBH55_002856"
     CDS             276..728
                     /locus_tag="NBH55_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510777.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            721..1119
                     /locus_tag="NBH55_002857"
     CDS             721..1119
                     /locus_tag="NBH55_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510778.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            1431..1504
                     /locus_tag="NBH55_002858"
     tRNA            1431..1504
                     /locus_tag="NBH55_002858"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1465..1467,aa:Ile,seq:cat)
BASE COUNT          657 a          248 c          318 g          585 t
ORIGIN      
        1 aatactccaa agatcggaaa tagtaaaaga acttttttat cataaattta gttatgaaat
       61 aagactagat gatacttatg agcattatag aattgttttt ttcgtataca attctataga
      121 agatgctact gcgactactg cattaacatt cggttttacg aaaaatggaa caatcaattc
      181 ggataaaacc cgtcaagctg ctacagaaag tgatgatata tgtaagaaag tatgcaatgg
      241 ggaagagaat tgctggatag gggaggaaaa gttgaatgaa atctattaat gatttattaa
      301 aaaaacatcc tgatttatat aaaaaattaa attctgaaca aagaaacctg tccaaacaat
      361 tacttaaaat aagattaaaa aaacaaaaaa acgtgacaga attctcaaaa atgttaggaa
      421 taagtgaaaa aaaatatctt gaatttgagt tcggagatat gcatattcct gttgcagagt
      481 acaataaact tttgaaacaa gttgaaaatt tgtcctataa agattcaatg aatttcacta
      541 ttaaggttgc tggcggccct attaaatatg accgtattga aaataaacct tatattcgga
      601 ataaaagtta ctcagtattt aaaagtagaa agagaaaaga agtatcgtca aatatggtct
      661 atgctagtgt taattaccaa gatacgagcc gctatattgt agaaaaagag gtatatgtac
      721 atggataaag gaaatagtgt tacttttgca cttgaaaata ttagtttgaa taaaattcat
      781 tttgaattaa ctgataattt tagttatgga gatttagagt ttaatattga acttggatat
      841 aaagaagacg aagcagagta tctagaaaaa aaacttgttt ctttacgagc tcacattgta
      901 ggggggatgg aaggagcacc ggtagatatt ttactctttt cagttattcg tgtttctaca
      961 aatgaacagc catataaaaa attagtagaa gaatctatgg gagagctagc taaacctatc
     1021 ctgaataaag cttctaattt gatttctaat ttatatagcg aagtaggagt tatgcctaca
     1081 gttattgatt tatatcagct atatagtcaa caaaaataaa atacaagtaa tagagttatc
     1141 tatttaaatt tgcaagacaa agacctcaac cagtcatcct tggttgaggc ttttttgctc
     1201 tgtaattaac tcgtatagat cctgcaagat agtttgtttc tgaataaatg attgatatga
     1261 agtatttaga tgactgtatt gacaactata aaaatcattc gataaaatag tgatgttatc
     1321 gcatatcttc actatcaccc ataatagtca cactccaagc tatgcgataa caggtttgtt
     1381 gccacacatt ctactggttg attgtttatg gctttatgtg gcaacaacca gtacccttag
     1441 ctcagttggt cagagcagac ggctcataac cgtccggtcg taggttcgag tcctacaggg
     1501 tacattaacg tagccatttg aatcgttctg tgttagaatt tttttgaaga gtattataca
     1561 agctaaagct tttcttcatt gccactcaaa tgagtggctt ttttatgtat ccttttatgg
     1621 attaatgaaa ggatgtttca catagttata cttctgtata tttgaaaagt tttactttga
     1681 tttttaaata gaaagacatt tgggttaaat tgtgagataa taataaagaa gagtttaaag
     1741 cgcaccccaa accacttccc cataagtgtg ttacgcttta aactctttta tatttgaagc
     1801 cattaaaa
//
LOCUS       DAHHPA010000202         1791 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.210, whole genome shotgun sequence.
ACCESSION   DAHHPA010000202 DAHHPA010000000
VERSION     DAHHPA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.210"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            178..375
                     /locus_tag="NBH55_002859"
     CDS             178..375
                     /locus_tag="NBH55_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510779.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            481..963
                     /locus_tag="NBH55_002860"
     CDS             481..963
                     /locus_tag="NBH55_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510780.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1021..1617)
                     /locus_tag="NBH55_002861"
     CDS             complement(1021..1617)
                     /locus_tag="NBH55_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCD1510781.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          558 a          275 c          358 g          600 t
ORIGIN      
        1 ttttttgaaa aaaattcttc taaatacaac agctgtttta attgtgaaaa cgaaaacagt
       61 acgctataat gcctgtaagg ttgattttta actgtaaata aaaatcaatg agttaaatct
      121 ccaaagaaat agcagtggta atgtggagaa aagttccagc agtttaggag gattttgatg
      181 acggaagaaa tgattaattt aggagaacaa tatgcatgca aaccaattgg ttttacaaaa
      241 acggtcattg gtgaagttgt atcaaaaatg acaaactgcg ccgtggtcaa agttgcccaa
      301 tgtgctgcag aggaccaaga attattagat gaaaaagcaa gcatggttgt tgcaaaatat
      361 gatacatttg aataaagaaa acaattggac gtgcggcgtg ctgcaagtcc aatttctttg
      421 agaaggtaaa tcagtattct tttcctattt tctggagaag aaaaagcgag gcgagttgtc
      481 atggtaacat tctttacggt tctattgttt ttattcgttg ttgtgatgtt tttatttctt
      541 tggctggttc gaaaagaaat tatttatcgg actgtgcgaa atcgatgggt ctacttggtg
      601 atacccttct tagtgtcact agtcatttgg tatacactca tcagtcatcc tacagcagat
      661 gaattggcaa aagggatctt gtcagcaatg atttttatta gttttttgtt ggacagtaga
      721 ggaatcacag aagaaggact tgttttgaat agctttgaca aaaaaggtgt tcctttttct
      781 gagatcaata aaattgtatt atatcagcca aaaggttcta aaatcgtaaa aatgaatttt
      841 ttccgaaacg gatggcgagg accgatgctg aaattttctg catcattaga agaattagtt
      901 ccatttttgt cccaacgctt aaacgatgag gcagagattg atattatgat tgatccagaa
      961 taaaaacaga ggttgcgaca agatgtttgt cacaacctct gttttttatt tcaaataaaa
     1021 ttatccaaat acgcggaagc gatcggcatc gttcatgtat tctttttccc cagccggtgt
     1081 ttctggtgaa ccgaatacta attgcgaacg tagtttccag tttgaaggga tattccattc
     1141 ttttgcaact gcttcgtcaa taactgggtt gtaatgttgc aagtttgcgc ccaatccttt
     1201 atcaactaat gctacccaag tattagctgt tgcgattccg tttgattgtt ctgaccaatc
     1261 tgggaagttg tctgcatata attcaaattg ttcttgtaga ccttttacca cgtcagtatc
     1321 tttgaagaat aatactgtac cataaccatt tttgaaacca gctaatttgt tttgtgtatt
     1381 tgggaaagct tctgctggag tcaatgggcg aagtgcttct tcagtgattt cccaaagttt
     1441 ttcatgtgca tctccgaaaa ggataactgc gcgtgttgat tgagcattga aagcagttgg
     1501 gctttcttta attgcttctt tgatcaaagt tgttaattct tcattagaaa gtgaaacatt
     1561 acgtcctaga tgatagattg aacggcggtt tttcaaagtg tcagtaaaag ttgtcataat
     1621 aaaatttctc ctttgatttg cgttttgcat ttttttaatt gattacgaaa tgaagtatag
     1681 caccttacaa aaagtaagta aacagatatg ctcacaaaaa ataagcgaag ttcaaaaaac
     1741 aattttaata agaaaaatcg ttatgtaagg attttcttag ttatcgcttt c
//
LOCUS       DAHHPA010000203         1726 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.211, whole genome shotgun sequence.
ACCESSION   DAHHPA010000203 DAHHPA010000000
VERSION     DAHHPA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.211"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(402..836)
                     /locus_tag="NBH55_002862"
     CDS             complement(402..836)
                     /locus_tag="NBH55_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510782.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(853..>1726)
                     /locus_tag="NBH55_002863"
     CDS             complement(853..>1726)
                     /locus_tag="NBH55_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCD1510783.1"
                     /translation="LYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTE
                     NLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPS
                     VSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVR
                     DSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESF
                     KNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPK
                     QYQLGLVDNFNNIS"
BASE COUNT          589 a          290 c          201 g          646 t
ORIGIN      
        1 atatgtatgt attctttctg aaattaagtt catttctatg cgatgaagca tgttttcaaa
       61 atattaccct ttcttagatt aaacttcaga gtattcaaat aacggaataa ggagccttat
      121 cccgcattac tgaatgagac tgtgacataa gtaaaaaggt gacttcaaat tccttcttcg
      181 gaatttgaaa tcaccttctt tataattata aagtatcggt ttcacagcat ttctgaatga
      241 ttcctagaat cttaaacgaa tgattggagt tatcaacatt ttttcactcg tttttagcga
      301 aacaaaaaat gacatttgtc acagcctcat taaattattg attctttttc tttataattg
      361 aattgtccat atttaaagtg tctagtactc tacatgaata attaattgct ttcactcaaa
      421 tacttataat ctattaactt gtagttgttt aacttttctg aaaaagttag acccacgtaa
      481 taatcttccc agctttctct ttcaattaac tgatccaatt tttgataatc cataaattca
      541 tcatgtatca aaaagaataa ataacttgat attttagcat ctagcttaat cagattaaat
      601 aatgtttgtt taaaattgct accattaata tgactgatag attctctagc ttcttcacat
      661 aaacttttta ctttattaat tactactttg tcatattcat ttaacacact attattacca
      721 ttacttagac gctcagattc tacgaaacaa ctcaaatcag aaatttccca aaaaaatttt
      781 ttgttaaaat ccaatttcct caactcctcc aaactacgta aatgatatag tctcataata
      841 aaataaaacc tctcagctaa tgttgttgaa attgtccact aaacctaatt gatattgctt
      901 aggagataca cctgcgtact ttttgaatac tctcgaaaag tgtgaagaat ctgaaaatcc
      961 taaacctaaa gaaacttcac tgatagtatg gtttgatttc aacattattt tagccgtaga
     1021 aatttttcgc ttagagatat aattataaat cgtaatatgc atttcattct taaagctttc
     1081 tcttaattta gtttcactta cagaaaaata attagctatt tcctttacag taattctttt
     1141 atagatattt aagccgatga attgaacgac agaagaaatt aagggagaaa gatgctctat
     1201 cttagttttt ccgatttcct tagtataata aacaatccca gaatccctca cttttaagac
     1261 ctcaggtaac gatacagcta attcgttttc ttgtatcaaa gcatctctac tttgatacgc
     1321 ttctattata tccattccta atgttataga cgtttgtgct aacttttcaa aaacaatgat
     1381 agaataattt ttttcagacc gaatcgtatc tccactaaca cttggaatta ttccattact
     1441 cagattaaaa accatgtttt ctaaatttcg aatatctcca tttttaacat attccagtat
     1501 tttattttca taaagaaaca gatatatttc tggatcgtaa ttttgtgata acagattatc
     1561 tattttgatt tgctcgatag ttttacttag attttctgta tattcaatta acggttcatg
     1621 gaatagatct tctatttttc cagtaaaaaa ataatgaact aaaatcagaa tatctcgaat
     1681 gtctcctaaa gaatacaaag tcaaattttg caatgaatta tagagt
//
LOCUS       DAHHPA010000204         1726 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.212, whole genome shotgun sequence.
ACCESSION   DAHHPA010000204 DAHHPA010000000
VERSION     DAHHPA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.212"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(24..1043)
                     /locus_tag="NBH55_002864"
     CDS             complement(24..1043)
                     /locus_tag="NBH55_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCD1510784.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1054..1260)
                     /locus_tag="NBH55_002865"
     CDS             complement(1054..1260)
                     /locus_tag="NBH55_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCD1510785.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(1393..>1726)
                     /locus_tag="NBH55_002866"
     CDS             complement(1393..>1726)
                     /locus_tag="NBH55_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD1510786.1"
                     /translation="ESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQR
                     GLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVS
                     IDDLSNLI"
BASE COUNT          493 a          358 c          284 g          591 t
ORIGIN      
        1 tgctcctcaa cgaggagcta tttttatcgt ttaggaatat ttaaatacca acgtttgtca
       61 tggaaatctt gcgcaccgcc tttagtgttt ccctctggat cattcgttgc ccgcatcatg
      121 acatagactt tcttattagg gaagttacgc atgttaaaag atacatgata gccaacattt
      181 ccagaagtat tataagcttg atttacatct gatctataaa ttccatcagc tcttactcga
      241 gctaattctt tcccagtatt gtaatccata atgaagatat actcgtattt atagttagca
      301 atgtgccatc cagctacatg caaatttgca ttttcgattt ctccaaactg atcaatgtgg
      361 gcgtaattcg ttccatctgt cagtgtagga ttagctgcac cagctcgtgt tggatcaatg
      421 acaggcttgt tttcagaagt tgttggattt tcatcggtaa atccatgagc taaatcatat
      481 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      541 ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt ataaggagtg
      601 tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      661 ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat ctgcacagga
      721 ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg accgacttga
      781 taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca tgaacgttgc
      841 atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg cagtaaaata
      901 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
      961 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
     1021 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
     1081 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
     1141 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
     1201 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
     1261 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
     1321 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
     1381 ctttcttaat tgtcaaatta agttagacag atcatcaata cttacgtaac tcaaaaatac
     1441 ttccaaaggt gttcgataat ccagtgattt tctaggaata ttatttcgtt tagatgcgac
     1501 agattgaata aaaaattcat ctactgaatt gaaatccata gatttcaata aaccatctct
     1561 acgtaacaat ccgttagaat tctcgtttag gcctctttga gacggcgttc ctggatcagc
     1621 gaaataaatg gcaatatcat tgacattact gatctgtttc caatttgaaa attcctttcc
     1681 acaatcaaaa gtgatggatt tgaataggtt tttcggtaca gattca
//
LOCUS       DAHHPA010000205         1723 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.213, whole genome shotgun sequence.
ACCESSION   DAHHPA010000205 DAHHPA010000000
VERSION     DAHHPA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.213"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            190..564
                     /locus_tag="NBH55_002867"
     CDS             190..564
                     /locus_tag="NBH55_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCD1510787.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(678..1307)
                     /locus_tag="NBH55_002868"
     CDS             complement(678..1307)
                     /locus_tag="NBH55_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCD1510788.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1441..1513)
                     /locus_tag="NBH55_002869"
     tRNA            complement(1441..1513)
                     /locus_tag="NBH55_002869"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1478..1480),aa:Thr,seq:ggt)
BASE COUNT          597 a          253 c          289 g          584 t
ORIGIN      
        1 ttttcttcta gtctacacac cttaattttt ctgtctagtt aattgtagcc tatccaagtc
       61 tacactctga gatgtaagaa atatcataag atatttctta catctttttg ttttgagcag
      121 gactttagat aattttatta atatatgcta gcatgctttt gtaagaaaaa gaaagagggc
      181 gtattagtta tgtatcttaa aattacggaa caagcacaag aaagattaca aaaatatatt
      241 gatgaaggcg caacagttat attggattta gatgatggcg ttggggaata ttctaaaatg
      301 ggtgtttgct ctttagatac tagttttcgt ttacttcttc ttgataaaga acagagaaaa
      361 aatgattata aactccaatt agatagtgat attggggatg tttatataaa agattattca
      421 aaaatgtaca tggatgaaca tatgaccctt tctttagatc cacgtctagg tgtatttact
      481 cttgaaagcc caagtggttc tttagacagt catgttcaat tagtagatct acgaccagaa
      541 aaattctctg gaataaaaca ataactttct ctctattgtc cataaaacta gtctcaatca
      601 agctgtattg aaaaaacaag acaacattct taccatggta aataaaaaga cgacacattt
      661 tgtcgtcttt ttttaaatta actattttcg ataaaagatc gcatagcttg tgccattatt
      721 tctggctttt ctctattcaa ttcatgtttt ccgtttttaa taaagagtag ccggccattc
      781 gttgaaagtt tatataaacg ttttgctgct ggtttattcg catgatcttt ggtaccgcat
      841 aaaatcaatt taggaagtcg tgatgtacga agctgttttt tgcagtctaa tgtttcaagt
      901 gaacgaagta gggtaagggt ttgtttttta gatagaccta atttttcaaa tatcttagaa
      961 ggtacgcagt aaaaataaat attctggaaa gtaagcaata agcgattagg agactcaaat
     1021 tgtggagcga ctaaaacaac cttttttaaa tatggagaag gagttgcaag atagtttaaa
     1081 gcaataacag cgcctaaaga taatccacat aaaataaaag gttcctttat ctgattcaat
     1141 tgtacttcga tgtatttggt taagtcgtct aaagtagcag tgtctgtgct gtttaaatga
     1201 ttgaacaatg aaagactttt gcaagaataa ctattgggta gataagagat tacatctttc
     1261 catgattgtt cgtcttggcc tagaccatgc aaaaaaacaa ttttcatatt gctctccttt
     1321 ttatagttat catatagact ttctttgtac tattaaaata taaacattca tttcttacgt
     1381 cataaaataa aaaaacagcg cagacacatt gttaaaccaa catgttgcac tgtttaagga
     1441 agccacttgc cgggcttgaa ccggcgacct cttccttacc atggaagcgc tctaccagct
     1501 gagctaaagc ggcattatca ttaatacaaa taataggata acgaatatta atgtatttgt
     1561 caacagaatt ttttcgaaaa aatgtgtttc tctaatagga aaacagattt tagaaaaaat
     1621 aaaaataaca aaaaatgcga acggtttctt tgataatata ataaaaaaat tcaaaaaaag
     1681 tattgcgtta tttagaagaa attgatatta tattcatgta ctt
//
LOCUS       DAHHPA010000206         1718 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.214, whole genome shotgun sequence.
ACCESSION   DAHHPA010000206 DAHHPA010000000
VERSION     DAHHPA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.214"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..319
                     /locus_tag="NBH55_002870"
     CDS             <1..319
                     /locus_tag="NBH55_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCD1510789.1"
                     /translation="PIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHS
                     LSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHD
                     ILI"
     gene            375..593
                     /locus_tag="NBH55_002871"
     CDS             375..593
                     /locus_tag="NBH55_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510790.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            664..1479
                     /locus_tag="NBH55_002872"
     CDS             664..1479
                     /locus_tag="NBH55_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510791.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            1543..>1718
                     /locus_tag="NBH55_002873"
     CDS             1543..>1718
                     /locus_tag="NBH55_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCD1510792.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKV"
BASE COUNT          667 a          198 c          291 g          562 t
ORIGIN      
        1 tccaattgat catatcatcc gacacagaac tttattgaaa gatttttcga attttgtttc
       61 tattaggtca gacggaggat taatggcatc accttacttg ccaattcaag ttggaaatat
      121 taaatgtcac agtctttcta aatattggcg agcaggactt tataaagttt tttctttaga
      181 tttagttcaa gatatagcta ctcaagtttc ttcaatacaa gatatgagta aagaattaaa
      241 aaaaggacat attaaatggc ttaatgaaga tatagaaatt gatattctcg atgaatacaa
      301 acatgatatt ttaatttagg tttatcattg gaaagtttag ataaacaaag tacttttaga
      361 aaggagggaa aaaaatgatt tctgaacctc aaatgaataa tatgtatgaa agcccaacta
      421 taatgggagc tggtgaagct gcgccaagag cttgttcagc aattatggtg tgtgtgttat
      481 tagcatctgc aggagtaggt atccaagcag gagcggtgta tacttatgct gtagttgcta
      541 cagtagctgt agctgcagcc gcagtttgga aaacagtagg attagttggt taggaattaa
      601 taggagacta actgtctcct attgattcca atgattaagc taaggaggaa ttttaagaaa
      661 ataatgaaat acaataattt aaaaccgaaa ttacttaatg agaacataga atatataaga
      721 gaagaaaatt atggatattt aaccatagtt cccaaaaatg gaactgaatt gaaagataac
      781 gttttcaatc caacagtaag atatatattg gctaactgta ctggaaaaaa cagtgtaaaa
      841 aaaatagcga atggtttaaa aaagaagttt cctagtttag aatatgaaaa attctatcaa
      901 gatgttgtag gaactattaa atttttcaaa cactataata ttattgatat aggagattat
      961 agtatggaag aaaagaaaat atataaaatt gaacaaaatt tagaattaga attactaaat
     1021 gaatcacatt atgaagaaat ttataatttt atcacagatc atcctgaaag tatgtatttt
     1081 catgaagata tttatcatca aggaaatagt aaagaattag aagttgtcct tagatataat
     1141 ttattttcgt ttacgagtga attttacttt ttaagacata aagagagaat tgtaggaatg
     1201 gtttctataa ttgttaatcc atttcaaaac ttatcctaca gtatagtaaa ttacctcata
     1261 tgtgagcctg catatactac gactattttg acaacagtat tagaaaatat taacgaagtt
     1321 tcaattttag aagtaaagaa gctagtgatt catttgctaa attcaacaga agatgaatca
     1381 ctaatcccct atttaaaaga ttcacatttt tttaaagaag cggtactatc taaagaatat
     1441 agagagcatg atgttttgag atacgcttac atttattaaa taaaatttct gatagtattt
     1501 taataattcg gatcatatca taagatatag gagggaaaag aagtgatttt attgattttg
     1561 tctctcgtat tactagttgt atctaatcta atgaaaaata atcctcctac aaaaattaat
     1621 tctttttatg gctttagaac taaaaagtca atgaaaaatg aaaaaagttg gcatttagca
     1681 caaaaattgt tgggggattt ttcaaaaaaa aaagttat
//
LOCUS       DAHHPA010000207         1711 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.215, whole genome shotgun sequence.
ACCESSION   DAHHPA010000207 DAHHPA010000000
VERSION     DAHHPA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.215"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..957
                     /gene="uxuA"
                     /locus_tag="NBH55_002874"
     CDS             <1..957
                     /gene="uxuA"
                     /locus_tag="NBH55_002874"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCD1510793.1"
                     /translation="WPRERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIE
                     NYKQTIRNLAEFGIEVICYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEII
                     RKVENSTNNFELPGWEPHRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGI
                     KMAVHPDDPPYSIFGLPRIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEI
                     LSEFCQRDRIPFAHVRNIKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYI
                     RPDHGRMIWGETGRPGYGLYDRALGAVYLNGIWEALEKGASK"
     gene            1031..>1711
                     /locus_tag="NBH55_002875"
     CDS             1031..>1711
                     /locus_tag="NBH55_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCD1510794.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVD"
BASE COUNT          574 a          242 c          360 g          535 t
ORIGIN      
        1 tggccgagag aacgaattaa agctttaaaa gatgaagtcg aagccaatgg ccttaccttg
       61 aaagttattg agagtgttaa tgttcatgat gatatcaaaa ttggtttgcc aacacgagat
      121 caatacatcg agaattataa acagacgatt cgcaatttgg cagaatttgg gattgaggtt
      181 atctgttata attttatgcc agtctttgat tgggtaaaat cagatttgga ttatcattta
      241 gatgatgggt cttctacgtt agcctatatt tcaaaaaata ttcctactga tccagaggaa
      301 attattagaa aagtagaaaa ttctactaat aattttgaat tgccgggttg ggaacctcat
      361 cggttatcac aaatcaagca attattagaa gcctataaag aagttgatga agcgaaattg
      421 cgagagaact ttgcatattt tctcaaagca attatcccga catgtgaaga agttgggatt
      481 aaaatggcag ttcatccaga tgatccacca tattctattt tcggattacc tcgtattgtt
      541 aaaaatcgtg acgatttaga atggttgtgt aatgtagtgg attctccttc taatggtatt
      601 acgttatgta ctggaagtat tgcagaacga ccagataatg atgtctatga aattctatct
      661 gaattttgtc aacgggatcg gattcctttt gcacatgttc gtaatatcaa atatttaagt
      721 gataatggac tcgattttta tgaagcgccg cataaatcag aatatggttc tctagatatg
      781 tataagatat taaaaacaat gtacgataat ggatttaatg gctatattcg tccagatcat
      841 ggtcggatga tttggggaga aaccggtaga cctggttatg gattatatga ccgagctcta
      901 ggtgcagttt atttaaatgg tatttgggaa gcgcttgaaa agggggcgag taaataatta
      961 tataagggag tttatttaga ggaaaaattt tctcttctaa aaactaacgg aaggaaagga
     1021 gtgtttaaat atgtttttag ataagaactt tttattagag aatgaaatag ctaaaagact
     1081 ttttcataaa tttgcaaaac accaaccaat tatcgactat cattgtcatt tagacccagc
     1141 atatttgcta gaaaataagc aatacaagaa tttgactcag gtatggttaa atgataaagg
     1201 agctggagat cattataaat ggcgattgat gcgagctaat ggtatagaag aagagttgat
     1261 cagtggtcca ggtgatgatt atgaaaaatt tttagctttt gtaaaaacag tggagaaggc
     1321 gccaggtaat cctatttttg aatggtctca tttggaatta caacgttact ttgatattga
     1381 tttgacaatt tctgagaaga atgcaccaga aatttggcga cgtgctaacg aaaaaattca
     1441 aacggattcc tatcgcccga aagaattgtt ggagatgatg gatgtgaaag ccatctgtac
     1501 aacggatgat cctacagatg acttgtggta tcatactgag ctttctaaac aggataattc
     1561 tttcaaggta ttacctactt tccgtccaga taaggtctgg agtatcaaat catcaggctt
     1621 ttcgaactat attttaaaac ttagtcaaac agcggctata ccaatcaaag actttaatgg
     1681 gttgaagcaa gcattgagcc aacgagtgga t
//
LOCUS       DAHHPA010000208         1685 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.216, whole genome shotgun sequence.
ACCESSION   DAHHPA010000208 DAHHPA010000000
VERSION     DAHHPA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.216"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(2..247)
                     /locus_tag="NBH55_002876"
     CDS             complement(2..247)
                     /locus_tag="NBH55_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510795.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(241..642)
                     /locus_tag="NBH55_002877"
     CDS             complement(241..642)
                     /locus_tag="NBH55_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD1510796.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(828..>1685)
                     /locus_tag="NBH55_002878"
     CDS             complement(828..>1685)
                     /locus_tag="NBH55_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCD1510797.1"
                     /translation="KDFNLMKWIGANSFRTSHYPYSEEIMRLADREGIVVIDEVPAVG
                     LHLNFMATLLDDNVEKHNTWKEIRTENSHKTVIKELIERDKNHPSVVMWSIANEPDSD
                     SEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDVCKVGDIVDVLCLNRYYGWYVS
                     GGDLAHAKYLLKKELDGWMERCPDTPIMFTEYGADTVAGFHDTVPTMFTEEYQVAYYE
                     ANHEVIDTCENFVGEQVWNFADFATSQGILRVQGNKKGIFTRDRKPKMIAYYLQKRWS
                     SIPNFSYKK"
BASE COUNT          519 a          329 c          221 g          616 t
ORIGIN      
        1 attagttaat tttaaatttt aagatcgctt ctattaattt aatttctagc tgacttatca
       61 tatattcatc tacataggta acgaattgac ctacctcgtt aaatccattt gtcaaacata
      121 acttagccat ataagaatga taatgtttga gtatcatctt gatcgaaatt ggatctcctt
      181 ctagagcaga taagataatg gatagtggaa ctaataggta ctgccgcttt ttagatttat
      241 ctaccatagg tcatacccca ttttcaattg ttcaactgcc ttctttcgtt gataccagat
      301 accacttgtg ctcatattga gaattttacc gatttcagca tcattaaaac ctgcaaaata
      361 ataaagtagg ataatctttc ttcttatctc cgataactga tcaattgctg ctgataactt
      421 ttgatctgaa attaatattt ccattccaaa taagaaatag acatcttgtt catcaaaatg
      481 agtttgggaa tacctgcctt catttgaatc tgccaatggt tcctcattat cctttatgtt
      541 agcgtttcgt tttcgaatag acttagcttc gtttcgtaat acttttttac agtaggcatc
      601 aaattggtgt tgtatagata tgtcattgta atgttgttgc atgataatat gctccttttt
      661 ccccctttcc actaatacaa caatcagaaa gccccttttt ttcgaaaaaa ctagaaaaaa
      721 aacataaaaa tatttatcct tctttaaaca gaaaatcttt ctataaaaaa caaaaaaaac
      781 gattaggcta ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa
      841 ctgaaattgg gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt
      901 ctatctctag taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt
      961 gcaaaatctg caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact
     1021 tcgtgatttg cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta
     1081 tcatgaaatc cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga
     1141 catctttcca tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc
     1201 cctcccgaaa cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct
     1261 accttacaaa catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc
     1321 ggatcaagtt ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa
     1381 tccgaatctg gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt
     1441 tctatcaatt ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta
     1501 ttatgttttt ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact
     1561 gcaggtactt catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct
     1621 gagtaaggat aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa
     1681 tcttt
//
LOCUS       DAHHPA010000209         1672 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.217, whole genome shotgun sequence.
ACCESSION   DAHHPA010000209 DAHHPA010000000
VERSION     DAHHPA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.217"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            130..>1672
                     /locus_tag="NBH55_002879"
     CDS             130..>1672
                     /locus_tag="NBH55_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCD1510798.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKK"
BASE COUNT          593 a          231 c          344 g          504 t
ORIGIN      
        1 aaaaagacac aataattctg acatggtaga aaaaagaata tttttagttt tttcttattg
       61 acagcgatta cattcgattt tatactacaa ttgtaacgga aactaacata tcagtatgga
      121 ggggtaaata tggttttgat taaagactgg caagattcta tcgaaggatt gaacaaaatg
      181 caagttcaac taccgtattt taatgtcgaa gcggtcaaac aagaaggtca gaaacatcca
      241 gtgtggcttc attttggtgg aggaaatctt tatcgaggat ttcatgcaga aattgctcaa
      301 tgcttagcag atcaaaatga attagaatcc ggagtagttg tgtgtgagac atttgatgaa
      361 gaagtagtag aaaaagctta tcatgactac aacaatgata ttttggagat tgttatgggt
      421 gaagatggtg aactcaaaaa aagactttta gcagtaacag ctgatagttt atattgcaat
      481 ccaaagaata taacggacta tcaaagagtg gttaaatatt ttgaagatcc tacattacag
      541 tttacaacgt ttactattac tgaaaaagga tacagtttaa aagattctaa aggacaattt
      601 ttttcggtag tggaagcaga tataaaatct ggacctacca atcctcaaca tacgatgagt
      661 attgtgacat ccttgttgta tagcaggttt aatgctggag agttaccgat tgcgatggtt
      721 tctactgata atttctcaca gaatggtcaa cgttttcaag atagcatttt tatgattgca
      781 gaagcttggc aaaaaaaagg atacgtttct gaaaaattta ttgcttattt aaaggataaa
      841 gaaaaagtga cttttccttg gagtatgatt gatagaatta cgccaaatcc ttcggaaagt
      901 gtcaaaatgg atttattgaa ggcgggtttc gaagacttag atataattca tactgcgaaa
      961 aataccaata tcgctgcttt taccaatact gaaaaagtcc attatctagt cattgaagat
     1021 agttttccaa atggtcgacc taaactggaa gcggctggtg tgattatgac agatagagaa
     1081 acagttgata aagcagatat catgaaggtt acgacatgtt taaatccgct ccatacggca
     1141 ttagcaataa caggttgttt gttggggtat aaatcaattc aggcagaaat gcaagatgat
     1201 gatttggttg gcttaattaa agggataggt tatgtagaag gcttaccagt agttgaaagt
     1261 ccaggcgtta ttgatccaaa acaatttatt gatgaagtaa tcaataaacg tttgcctaat
     1321 ccctatattc cagatacacc gcaacgaatt gcaacagata cttctcaaaa agtagcgatt
     1381 cgctatggtg aaacaattaa aaagtatgtc gaaaaagacg gaaatgcaga aaatctaaca
     1441 tttattccta tcgctattgc tgcttggtta cgttatttat tggcagtcga tgatcaagga
     1501 aaaaattttg tgccaagtcc tgatccttta ttggtagagt tgcaggaaaa attctcagta
     1561 ttgaaattag gtgaacaaga tcctgcaaag attcattgtg cggtacagga aatcttagaa
     1621 aataaggaaa tatttggttt agatttatat caagttgaga taggaaagaa aa
//
LOCUS       DAHHPA010000210         1672 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.218, whole genome shotgun sequence.
ACCESSION   DAHHPA010000210 DAHHPA010000000
VERSION     DAHHPA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.218"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..1408
                     /locus_tag="NBH55_002880"
     CDS             <1..1408
                     /locus_tag="NBH55_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1510799.1"
                     /translation="TVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNT
                     PGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVT
                     NLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNAD
                     KYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYP
                     DGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTW
                     KEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGK
                     TEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVV
                     VSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLI
                     LLGTLGLLGYKNRKKENE"
     gene            1542..>1672
                     /locus_tag="NBH55_002881"
     CDS             1542..>1672
                     /locus_tag="NBH55_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCD1510800.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     "
BASE COUNT          660 a          250 c          361 g          401 t
ORIGIN      
        1 aacagtagaa ggcgaaaaag tagaagttgg gggcacagta gatctaacag ataatgttac
       61 taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat
      121 tgataccaac acaccaggta attacgaagg tgtcattgaa gtaacgtatc cagacggtac
      181 gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctgacg ccgataaata
      241 tgaaccaaca gtagaaggcg aaaaagtaga aattggaggc acagtagatt taacagataa
      301 tgttactaac ttgccagcgt taccagaagg aacaacagta acagatgtta ctcccggcgg
      361 tacaattgat accaacacac caggcaatta tgaaggtgtc attgaagtaa cgtatccaga
      421 cggtacgaaa gatacggtga aagttccagt agaagtgaca gacaatcgtt ctaatgcaga
      481 taaatacaat cctgaaggac aaaaagtgac tacagactta aataaagagc cagatgcatc
      541 tgagggaatt aagaacaaag aagatttacc aaaaggaact acttatgctt ggaaagagaa
      601 agtagatgtt agcacagctg gaaataaaaa aggtactatt gtagtgacat atccagatgg
      661 atcatctgat gaagttgaag tagacgttac agtgacagat actcgttccg atgcagataa
      721 atataatcct gaaggacaaa aagtgactac agacttaaat aaagagccag atgcatctga
      781 gggaattaag aacaaagaag atctaccaaa aggaactact tatacttgga aagaaaaagt
      841 agatgttagt acagctggaa acaaaaaagg tactgtagta gtaacatatc cagatggatc
      901 taaagaagaa gtagaagtta ctatttctgt agtagataaa aaagcaccga ataaacctca
      961 agttgatcct attacagagg gtgaccaaat tgttactggt aaaactgaac caaatgcaga
     1021 ggtgacagtt acattaccag acggaagtca acaccatggg acagctgata aaaatggtaa
     1081 ttttacagtt aaagttccta aattagaggc agggacaaaa gtgatagtaa ctgcaactga
     1141 tgaatctggt aatactagtg aacctactaa tgtagttgtt tcttccaaca aaaaagacag
     1201 tggaaaaaac ggtagtaaag gtagtaagac tgacaatcaa ggtagcaaca gcaatcaaga
     1261 caaaaataga ggcaaaagcc agtcctcaaa agtttttcct aagacgggag agagtgacag
     1321 taatatcttt actattagcg gtggtttaat actgttagga acgttaggat tattagggta
     1381 taaaaatcga aaaaaagaga atgaatagaa aatcaatttc ttaagtcttc tctgagaaga
     1441 ggaagaatct ttttcttcct ctttttgctt ttttatgatg ggaaataagt gttagttaaa
     1501 gatgaatata ctttttatag aaaggaaaag aatgtaatta agtggaaaaa atagtaatta
     1561 ttggttcaaa ccattcaggg ctagccgcag ctcgatttct aaacttaagc gattcaaatt
     1621 atgaagttat tctaatcgat aaaaaatcag aaattagtta cttggaatgt gg
//
LOCUS       DAHHPA010000211         1591 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.219, whole genome shotgun sequence.
ACCESSION   DAHHPA010000211 DAHHPA010000000
VERSION     DAHHPA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.219"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(31..408)
                     /locus_tag="NBH55_002882"
     CDS             complement(31..408)
                     /locus_tag="NBH55_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510801.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(708..1589)
                     /gene="galU"
                     /locus_tag="NBH55_002883"
     CDS             complement(708..1589)
                     /gene="galU"
                     /locus_tag="NBH55_002883"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCD1510802.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          435 a          358 c          241 g          557 t
ORIGIN      
        1 ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg
       61 ttcgagagaa tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga
      121 ctttcttttc atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa
      181 tacgcaagct tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga
      241 tgctgcatac cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat
      301 aaatagacta ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca
      361 cttgagtata tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca
      421 ccttttttta taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct
      481 ttcatttctc ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag
      541 cagaatattt tctgactcta ttcacgaaag tctttttcta tctttcgttt tccacgactc
      601 aactgatcta gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc
      661 tttaataaca aatagccttt cattctcagc aggccactta tttccagtta actttctgtt
      721 cttagctttt tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt
      781 tgaagtccat attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt
      841 tttcctttaa actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc
      901 gttagctgta tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta
      961 ggagtcagta gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt
     1021 ttttctacaa acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata
     1081 attccgtatt tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt
     1141 gtttcttcat aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca
     1201 tcccccagca tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca
     1261 tcccccagtc ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct
     1321 tcaactaaag cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata
     1381 tttgcatcaa agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct
     1441 tcgattcccg aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt
     1501 ggaagcattt cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg
     1561 gcaggaatga ctgctttttt gaccttcact t
//
LOCUS       DAHHPA010000212         1569 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.220, whole genome shotgun sequence.
ACCESSION   DAHHPA010000212 DAHHPA010000000
VERSION     DAHHPA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.220"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>1569
                     /locus_tag="NBH55_002884"
     CDS             <1..>1569
                     /locus_tag="NBH55_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCD1510803.1"
                     /translation="LEEKIGQLFVNMGSSRDKNYLSNMVNQYHIGAVRYNPGTADEVY
                     DQNYILQTESKIPLLIAANTEAGGNGACTDGTEVGVEVKIGATNDAKYAYELGRVSGI
                     EASAIGCNWSFAPIVDINRNWRNPIISSRSFGQNPDKVLELSLAYMKGIQESGILPAA
                     KHFPGDGIDERDQHLSFSINSLGTEEWDNSFGKVYSGLIEAGLPSIMAGHIHMPAYSK
                     HFNPDLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDASHMLGMTGAMKRSELLPTS
                     IAAGCDLFLFFNDPDEDFNYMMEGYRKGIITDDRLLDALKRILGLKAKLGLHKKAKEQ
                     ILLPKQEALEKIGLIENKSIFKEVADQAITLVKDKQDIWPIVPEKYKRILLVDVHGVK
                     GGFGEVVAGNRPQAIDLLKQILSAKGFEVEKYISPEEELKGLSQEEKIKNIAGIYAAK
                     RPIKNLTKKYDLIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIPTIFVSVQCPFHL
                     ADVPQVKTYINTYDG"
BASE COUNT          557 a          207 c          316 g          489 t
ORIGIN      
        1 tttggaagaa aaaataggtc aattgtttgt taatatggga tctagtcgtg ataaaaatta
       61 cttgagtaat atggtcaatc agtaccatat tggagctgta cgatataatc caggaactgc
      121 tgatgaggta tatgatcaaa actatatctt acaaacagaa agtaaaattc ctctattaat
      181 tgctgctaat acggaagcag gtggtaacgg agcttgtaca gatggtactg aagttggtgt
      241 agaagtaaaa attggtgcaa caaatgatgc gaaatatgct tatgaattag ggagagtttc
      301 tgggattgaa gcatcggcta ttggatgtaa ctggagtttt gctcctattg tagatattaa
      361 tagaaattgg agaaatccta tcatttctag tcgatctttt ggacaaaacc ctgataaagt
      421 tttagaactt agtttagctt atatgaaagg tattcaggag agtggaattt tgcctgcagc
      481 taaacatttc cctggtgacg gtattgacga acgagatcag catctatctt ttagtattaa
      541 tagtttaggt acggaagaat gggataattc ttttggaaaa gtatactcag gcttaataga
      601 agctggatta ccttcaatta tggcaggtca tatccatatg ccagcttatt caaaacattt
      661 taatcctgat ctgaaagatg aagatttact tcctgcaact ttatcaaaag aattaatcac
      721 tgacttactt agaggaaaac tcggatttaa tggattagta gttactgatg ctagtcatat
      781 gttaggaatg actggggcaa tgaaacgatc agaattattg cccacttcta ttgcagctgg
      841 atgtgatcta tttctatttt ttaatgatcc agatgaagac tttaattata tgatggaagg
      901 ctatcgtaaa ggaattatca ctgatgatcg gttattagat gccttaaaaa gaattttagg
      961 gttaaaagct aaattgggat tacacaaaaa agcaaaagaa caaattttat tacctaagca
     1021 agaagctctg gaaaaaattg gtttaattga aaataaatca atttttaaag aggtggctga
     1081 ccaagcaatt acattagtta aagataaaca agatatttgg cctattgtac cagaaaaata
     1141 taaaaggatt ttactagtag atgtacatgg ggtaaaaggt gggtttggtg aagtagttgc
     1201 tggaaataga cctcaagcca ttgatttatt gaaacagatt ttgtcagcaa aaggatttga
     1261 agttgaaaaa tacatttcac ctgaagaaga attgaaagga ttatcgcaag aagaaaaaat
     1321 taaaaatata gctggaatat atgctgctaa gagaccaatt aaaaatttaa ctaaaaaata
     1381 tgacttgatc attaatatag ctaatgttaa tccaggcaca gttcaacgaa ttgtctggcc
     1441 agcaagtaaa ggaactccgg atataccgtt ttacattcat gaaattccaa caatttttgt
     1501 atctgtacag tgtccgtttc atttggctga tgtacctcaa gtaaagactt atattaatac
     1561 ttatgatgg
//
LOCUS       DAHHPA010000213         1548 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.221, whole genome shotgun sequence.
ACCESSION   DAHHPA010000213 DAHHPA010000000
VERSION     DAHHPA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.221"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(400..1014)
                     /locus_tag="NBH55_002885"
     CDS             complement(400..1014)
                     /locus_tag="NBH55_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCD1510804.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          517 a          291 c          251 g          489 t
ORIGIN      
        1 ggacaatttt gttatatcag gtaaacttca ttgcaataat gataacaaat gtactataat
       61 tactttgtaa agagttttgg ggaagactct ttcatcaacg tgtattaaga gacaagaaag
      121 aatccatatg gattctttct tgtctctttt tccactactt tttatttttc tgacactaca
      181 tattcttata actcatttct cataactgtg aaacaactta taaaaataac aaactttaaa
      241 caaaacaagc ttaaaatcaa agatgatcca tcttaaaata actaaaacaa aaaaagtagt
      301 cgttgttaat cctcattaat aatcgttttc tttttttcaa acgctccatt tttatgtgga
      361 acattatttt tttaaggaac tttgtcaaca tgtggataac tattttccac ttgtgtcctg
      421 gactagctgt tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg ttgcaagaga
      481 ggctgttccg cagatcgctg gatctagatt tggcaaagtc aagactgcat catgtaaatt
      541 ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac tgctatgttg
      601 cgatttgata cttcgtgaca aatcaaatcc agtaacaatg attaatggat gaccattttc
      661 tttgatccac tgagctagtt cgatgattgc cgggtttctt ccttcattcg aaagaagaag
      721 aaaaatgtct tctgatttga cagaaagctg atcaataatt gcctgtcctg caccttctag
      781 cgtttcatag acacctccgg ctggattttc aatcaatata cttggtacaa gatccggggt
      841 ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg ctagcatata tctgcaatct
      901 accaccttga ttaatttgtt tcgcgatcca agtgccagct tgaagcatca gcggctcttc
      961 tatccttaat ttctcttcca attggtgata atagtcaaaa taacataaac tcatttatct
     1021 ctcccgccta gctatacttg acatgaatag aaacagctgc ctaatctaag gaagtacata
     1081 aaaaaacagt agttagacag taacacatgt ggtctttgct gtttcaaaca caaaaaaggc
     1141 attgaacgaa tacgcccaat accttgacat agcaaataaa acaacgtgca gaccatggaa
     1201 aatatctaca taggggtaga attttcaccc tagattaggt acaataaatt aggtcgatgc
     1261 actgcattga ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg gtcattccct
     1321 gattcttagc gccaactaag ggtcagggag ccttttttta ttcagctgtt tctatctgaa
     1381 tttattgtaa gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa acattggtat
     1441 gatttatata tatataaata acataaaaaa aggaatggct tatcccgata caacaagcat
     1501 tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccac
//
LOCUS       DAHHPA010000214         1545 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.222, whole genome shotgun sequence.
ACCESSION   DAHHPA010000214 DAHHPA010000000
VERSION     DAHHPA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.222"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            35..1501
                     /locus_tag="NBH55_002886"
     CDS             35..1501
                     /locus_tag="NBH55_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCD1510805.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          426 a          290 c          326 g          503 t
ORIGIN      
        1 ttttctgaaa atttaaagaa ttggggtata gattatggaa aaacagcaag acaagggctt
       61 cttcgggcag ccgaagggat tgtcaacttt gttcttcact gagatgtggg aacgatttag
      121 ttattacggt atgcgtgcat tacttatcta ttatatgtac gatacagttg ccaacggggg
      181 acttggatta ccacgagcaa cagcattagc tatcatgtcg atctacggat ctttgatcta
      241 catgtctagt atcgtcggcg gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt
      301 attcttcggt ggagttttca ttatgcttgg ccacatcgtc ttggctttac catttggtgt
      361 aagtacattg tttgtttcga tggcattgat tattatcgga accgcctttt tgaaaccaaa
      421 tgtttctggc atggtcggac atctttattc aaagactgac ttgcgaagag atgcaggatt
      481 ttcgattttt tacatgggtg tcaacttagg ggcattatta gcaccactaa tcgttggtac
      541 aatcggtcaa gaatataact atcatttagg gttctcttta gctgctattg ggatgttctt
      601 tggattatta caatacatca tccaaggacg caagacatta gacggagtgg gtacaacacc
      661 gccgaatcct ttgacaactg aagaacgtaa aggattcatc cgtaacttga tcatcgcact
      721 tgttgttatc gctgcagtat tcggcggggc acaactaact ggtcatctga caattgactt
      781 ttttgtcaat acaatcagtg tattggggat tatcttaccg ttgtattact ttatcaaaat
      841 gctgacttca aaagatgtaa ctgctgaaga aaaaccaaga gtatgggctt atatcccatt
      901 attccttgca gcaatcattt tctggtcatt acaagaacaa ggttcatcta tcttagcttt
      961 atttgctagt gaacgtacac aatctacgct atttggtgta acgatccaag caagctggta
     1021 tcaatcattg aatccaattt tcatctttat tctgacacca gtattcgtaa cgatgtggac
     1081 gaaacttggt gaacgtcaac catctacagt agtgaaattc tctcttggac ttttatttgc
     1141 aggattatcg tacttgctat tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt
     1201 aagtccattg tggttgttgg gaagtttctt catcgtggtt attgctgaat tgtgtctttc
     1261 accagttggt ttgtctgtga caacaaaatt agcaccacga gcatttgaat cacaaacaat
     1321 cgctatttgg ttcttagctg atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt
     1381 ctacacaccg ggaacagaat ctgcttactt cggtattatc ggtgcagtag caatcgtcgg
     1441 tggtatcttg ctatttactg tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta
     1501 atcaatgaac ataaaaacag gaattgctta tttgcagcag ttcct
//
LOCUS       DAHHPA010000215         1533 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.223, whole genome shotgun sequence.
ACCESSION   DAHHPA010000215 DAHHPA010000000
VERSION     DAHHPA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.223"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            152..409
                     /locus_tag="NBH55_002887"
     CDS             152..409
                     /locus_tag="NBH55_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510806.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            423..755
                     /locus_tag="NBH55_002888"
     CDS             423..755
                     /locus_tag="NBH55_002888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510807.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            800..1348
                     /locus_tag="NBH55_002889"
     CDS             800..1348
                     /locus_tag="NBH55_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCD1510808.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            1355..>1533
                     /locus_tag="NBH55_002890"
     CDS             1355..>1533
                     /locus_tag="NBH55_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510809.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIF"
BASE COUNT          549 a          165 c          327 g          492 t
ORIGIN      
        1 gaaaaaaaca gactgttttt ttaagaaaaa ctatgtcatt ctttacttga aaaaagttgt
       61 tataggacag aaaggataaa acaaaaacgg aaaaaatatg tcttatgaat aactataaga
      121 actattctat tttttaggag atggatacga gatgaaaaaa ctaattggct atagtgtgat
      181 gtgtattgga tttgttggtg tgggattgtt tgtatcgact gaatctgctt ctgcggctga
      241 atggtataat cctatctggt ataatccgaa cattgaatgt caaaaaggct atccgtatgg
      301 accgacaaat ctgaaaaatg gaaaatgtag agtaaattgg aatggcgttg tagatagtgt
      361 aattactggt tcaatggact ctgctctaca tggctttacg ggtcactaga aggaggaaga
      421 aaatgtctga agagaaaaaa gaaatcgttg aatcattagt agctttaaaa gattctcttt
      481 ctaagattga atatgttgat aggcaagaaa ttcaaaaact aattgatgat actattattg
      541 aaatccaaga tgcgcgttgt gaaggtataa aaatttctgt agctttatct aaagtaatag
      601 aaaaaatgaa ccgttcatta gcgtttaatg gattgaagct agatagacaa actagtctga
      661 tatgggatca tttaaaagac ttgtatgaca agtctaaaag aagtgaacgg actgctgtat
      721 caatcttaaa aggcctttgg ggaatgaatt cataaacaag atatttaaaa agtaacttat
      781 tttttatgga ggagaaatta tgaaaagaat aattaaaata tttggtgttt taggtgtttt
      841 cttattaggt ttaagtactt ttgttagtac tggaacaagt gtgtatgcca cagaaaaagt
      901 gaatacagtt gatttagaaa taaagaataa attagtagat gaattaactt tttattttga
      961 tacagttggt tttttagacg aacatggtgt ataccatata aaggattttg gtgctattcg
     1021 tgaacgaatc agtcaagata acgaacaagg aagagcagca agagtctttt atgaacatat
     1081 tattgttcct agaatggaga aagttgattg gaaacgatat gctggatgtg tagccattaa
     1141 ttctcttcct ggaggaggag tggcttgggc tataggtacg cagggagatt ttattgaagc
     1201 attaaaagct tttaattttg aaaaagctgc ggatttatta ttaaaaattg cagaaaaaca
     1261 attaggaaaa gctgcagcta aagagttagc taaggtaaat attgtagcag gaattgcagc
     1321 agcagctgct agctgtgcat tagagtaaaa gtttatgaaa agtttagaac aagagcatat
     1381 ggcttttaaa caagcaatgt ttaaagaaaa tatctattta aaccataact atattcgtgt
     1441 ttctaaggca tgttcaccag tattgaatat gcttggaggt ggaaatggat tatatcattt
     1501 actgtttgtc gatgtttgtt ggttaatttt ttt
//
LOCUS       DAHHPA010000216         1512 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.224, whole genome shotgun sequence.
ACCESSION   DAHHPA010000216 DAHHPA010000000
VERSION     DAHHPA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.224"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..600
                     /locus_tag="NBH55_002891"
     CDS             <1..600
                     /locus_tag="NBH55_002891"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HCD1510810.1"
                     /translation="AVVRGKNKDEAIKAINSIIAGGIKGIELTFTVPNAEEIIKEIAK
                     KYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTAEICNLYQIPYLPGCMT
                     ITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMPTGGVSLENLEDWFEVG
                     VVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            720..>1512
                     /locus_tag="NBH55_002892"
     CDS             720..>1512
                     /locus_tag="NBH55_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1510811.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYV"
BASE COUNT          499 a          196 c          309 g          508 t
ORIGIN      
        1 gcggttgtac gtgggaaaaa taaggatgag gccattaaag caataaattc aattattgcg
       61 ggtggaatta aagggattga actaactttt acagttccta atgctgaaga aataatcaaa
      121 gaaattgcta aaaaatatgc tgatcaaaaa gatgtaataa tcggagccgg tacggtttta
      181 gatgcgttta ctgctcgtat cgcaattatg gctggggcag aatatatcgt tagtccatct
      241 tttgatcgac aaacagctga gatttgtaat ttatatcaaa ttccttattt accaggttgt
      301 atgacaatta ctgagataaa ggaggcactt actgctggcg tagatatcat taaactattc
      361 ccaggttctg cttttggtcc atccattgta aaagcctatc aagctccatt accacacgta
      421 aatataatgc ctactggcgg agtaagtcta gagaacttag aagattggtt tgaagtagga
      481 gttgttgcag ttggtgtagg tgggaattta ttggcttctg ttgaaaaagg tgattttgaa
      541 gaagtaagtc gagtagctag taaatatata gataagtttt atgagataaa gaattattag
      601 gagaaaaaac aaataatact ctgaaatgta aaaaataaat actgactctt tacgcttcag
      661 agtatagtac gtactttcat cagaatgaaa acgcttaaca acagaaggga gagaattgta
      721 tggaaagaaa atttggtata agagacaaaa taggatatat gtttggagat tttggaaacg
      781 attttacttt catttttgct agttcttttt taatgatctt ttatacaaaa gtattgggga
      841 taagtggagc tgccgttgga acattatttt tagtagcaag atttatcgat gcagttactg
      901 atgtaactat ggggcgtatt gttgatagat cgaaagcaac taagcagggt aagtttaaac
      961 cgtggatttt aagaatgagt ggtccagtcg ctttagcaag ttttttaatg tatcaaactt
     1021 ctatgatttc agcttctatg agcgttaagg tagtatacat gtatgttacg tatatattat
     1081 gggggagtat tttttacaca tctataaata ttccttatgg atcaatggca tcagctatta
     1141 cttctattac agaagaaaga acaatgctat cgacattccg aaatgttgga gctactttag
     1201 caagtttggt cattggtgta ttaacaccat tatttattta tacaagagat gattcaggcg
     1261 ctcaaatagt tcgtggtgga tcaactttta caattgttgc gggaatattt tcgatatgtg
     1321 cacttatttg ttatttaata tgttataaac taactactga acgagtaaga attgaccaat
     1381 ctcaatacaa agtttcattg ttaaaatctt taaaagggtt agttaaaaat agggcattac
     1441 tttctataat tggtgcggct attttcttac tccttgctca attattaatt atgagtatga
     1501 ataattatgt at
//
LOCUS       DAHHPA010000217         1503 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.225, whole genome shotgun sequence.
ACCESSION   DAHHPA010000217 DAHHPA010000000
VERSION     DAHHPA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.225"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..770)
                     /locus_tag="NBH55_002893"
     CDS             complement(<1..770)
                     /locus_tag="NBH55_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1510812.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLT"
     gene            complement(899..>1503)
                     /locus_tag="NBH55_002894"
     CDS             complement(899..>1503)
                     /locus_tag="NBH55_002894"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HCD1510813.1"
                     /translation="IAVVRGKNKDEAIKAINAITAGGIKGIELTFTVPNAEEIIKEIA
                     KKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTAEICNLYQIPYLPGCM
                     TITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMPTGGVSLDNLEEWFEA
                     GVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
BASE COUNT          494 a          311 c          194 g          504 t
ORIGIN      
        1 gttaaaagct gagcaagcaa caaaaaaata gctgcagcta ttatagaaag caacgctcta
       61 tttttagcta atccttttaa agatttcaac aatgaaactt ttaactgcga ttcatcactt
      121 ttaacccgtt ctacagttaa tttataacat attacgtaac aaataactgc acacactgaa
      181 aatactcctg ccacaattgt aaaagttgat ccaccacgta caatttgagc tccagaacta
      241 tctcttgtat aaataaaaat tggtgttaac acgccaatga acatacctgc taaggtagct
      301 cctatactac gaaatgttga taacgctgtc ctttcttctg caacagaagt gatagctgat
      361 gccattgaac cgtaaggaat attaatagat gtataaaaaa tacttcccca tagtatatat
      421 gttacataca tatatataac tttaatactc atagaagctg atgccattga tgtttgatac
      481 attaaaaaac ttgctaaagc gactggacca ctcatccgta aaatccacgg tttaaatttg
      541 ccagttttag atggttttga tccatccaca atacgcccca ttgttatgtc tgtaactgca
      601 tcaataaatc ttgctactaa aaataatgtt cctacggtag ctccgcttat ccccaatact
      661 tttgtataaa aaatcattaa aaaagaacta gcaaaaatga aagtgaaatc attcccaaaa
      721 tctccaaaca tatatcctat tttgtctctt agaccaaatt ttctttccat ataatttgcg
      781 ctccttccta ttgttaagcg ttttcattct gttgaactgc caacttacgt actatactct
      841 gaagtataaa gagttagtat ttatttttta cattttatag tattatttat tttttctcct
      901 aataattctt tatctcataa aacttatcta tatatttact agctattcga gttacttctt
      961 caaaatcacc tttttcaaca ggagccaaca aattcccacc tacaccaact gcaacaacgc
     1021 ctgcttcaaa ccactcttct aagttatcta gacttactcc gccagtaggc attatattta
     1081 catatggtaa tggagcttga tacgttttta caacagatgg accaaaagca gaacctggaa
     1141 atagtttaat gatttctact ccagcggtaa gtgcctcctt tatctcagta attgtcatac
     1201 aacctggtaa ataaggaatt tgatataaat tacaaatctc tgctgtttgt cgatcaaaag
     1261 atggacttac gatatattct gctccagcca taattgcaat acgagcagta aacgcatcta
     1321 aaactgtacc ggctccaatt attacatctt tttgatcagc atatttttta gcaatttctt
     1381 tgattatttc ttcagcattc ggaactgtaa aagttagttc aatcccttta attccacccg
     1441 cagtaattgc atttattgct ttaatggcct catctttatt ttttccacgt acaaccgcaa
     1501 tga
//
LOCUS       DAHHPA010000218         1502 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.226, whole genome shotgun sequence.
ACCESSION   DAHHPA010000218 DAHHPA010000000
VERSION     DAHHPA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.226"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..848
                     /locus_tag="NBH55_002895"
     CDS             <1..848
                     /locus_tag="NBH55_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCD1510814.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
     gene            1389..>1502
                     /locus_tag="NBH55_002896"
     CDS             1389..>1502
                     /locus_tag="NBH55_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCD1510815.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          496 a          266 c          291 g          449 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaatttta
      961 tttatggtat gacataaata aagtaaagtt attcttctag gttaatctta ttaatactct
     1021 ttctcactag tgttgcatgc aacactagtg agaaagagta ttaaatatac tgcaaggcaa
     1081 gacatttggt aattgaagaa taacaagcaa aaaaatagca gtcaggtctc ttgacaaagt
     1141 atagttgcta ttcttttttt aggccctact ttaatgaaat ttttgtttgg agggaacgac
     1201 ttcattcctt tgattatttc agttgaattt aatagagaag cctggcgtct attaagaaat
     1261 acttttcttg atgagcgtca atttttttaa cggaaatcta tcctatacta aattcggttc
     1321 tgttgcaaag ttttccaaaa aatctatttt agtgtaaaat tgagaaaaaa gacagagagg
     1381 acagagtaat gaatcatttt aaaggcaaac aattcaaaaa agacgtcatt attgtcgctg
     1441 ttggttacta cctgcgttac aatctaagct atcgtgaagt tcaggaattg ttatatgatc
     1501 gt
//
LOCUS       DAHHPA010000219         1445 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.227, whole genome shotgun sequence.
ACCESSION   DAHHPA010000219 DAHHPA010000000
VERSION     DAHHPA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.227"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            39..1208
                     /locus_tag="NBH55_002897"
     CDS             39..1208
                     /locus_tag="NBH55_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCD1510816.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          519 a          263 c          234 g          429 t
ORIGIN      
        1 tctttctgta aaaaagaaag gagactaaat aaactattat gaaaaaagga aaacatgcta
       61 aaaaaagaaa atattcattt ttaatgatcg ttgtcgtgtt gtttagcctt agtgcgttct
      121 ctacaggaac tattcttgga tggcaatttt acaataatga acactctcag atagttgccc
      181 atgctcaatc agctcaaaaa aagttgatac tgactaccaa tgaacaacag ctcgttgaaa
      241 aaatcgatca actattaaga aaaaatgatt atattggctc aatttacgta aagaaaaata
      301 atcgcatcat catggaaaaa ggctatggct atgcgaataa actcacgcaa caaccaaatg
      361 atccttctct ttattatcag attggttcgg ttcaaaaagc attgactact cttttgattt
      421 taaaacaagt cgagcgtggg cacctttctt tagatactaa gctggctcaa ttctatccac
      481 aaattgctgg cagccagcaa attactatcc gtgacttgct gtatatgcgc tcaggcttgc
      541 accgaactgc ttctccaacc ataccgatga ctgatgaaga agtcgttcaa tttgcgattc
      601 agcacttaaa atttacagat tatcagacct ctcattatga ttcattgaac tttaccatat
      661 tgactggaat tttgatcaaa ttaacaaatc aaccgtatga aaaattattg accgatgata
      721 tcatcgaacc tttaggatta cagcatacaa aattttatga ccaagtcaaa aattcatcca
      781 atcatgcact atcctatcaa atgtctaaag aggatgatta ctttaaacca ctgacagaat
      841 ctgaaacaga gatccataac gaattaggga ctggtaatat cagtatgtcc gtctatgatt
      901 taaatacgct gtttacacaa gttttatctg gaaaactagt ttccaaagac ttactcttct
      961 cattatggcg tgaaaatgcg aatggacatc catatagtgg tggggtatat agcggagata
     1021 attatatcct gtcgcaagga aatatcgact cttttgatac atctgctata tttagcaaag
     1081 attcacagga tgctatcatc atggaaagca atataaaagc tgacaaaact atcaaactgc
     1141 ctactgctga tctaagaaac caaatctatg acctgatgga aggaactact aagttggcaa
     1201 atagctgata tgatgaaaag atagcaaata ctacttcttt gaatagcatt tgcttaaaaa
     1261 aagtactcga ctaaacgtag gaaagagtgt gggacaaatc taaaaatcaa tttttgctac
     1321 agactttaat tccaaataaa cagaacagaa agaggtgttc ttctttctgt tctgtccaaa
     1381 ttattctaag atttctcttt accaacatta tttgacaaag ggcctattaa atctaaaata
     1441 gtatc
//
LOCUS       DAHHPA010000220         1445 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.228, whole genome shotgun sequence.
ACCESSION   DAHHPA010000220 DAHHPA010000000
VERSION     DAHHPA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.228"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            8..418
                     /gene="spx"
                     /locus_tag="NBH55_002898"
     CDS             8..418
                     /gene="spx"
                     /locus_tag="NBH55_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCD1510817.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            432..788
                     /locus_tag="NBH55_002899"
     CDS             432..788
                     /locus_tag="NBH55_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCD1510818.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          532 a          218 c          267 g          428 t
ORIGIN      
        1 tgagaaaatg ataaaagtat atacttcgcc tagctgtact tcctgtagaa aagcaagagc
       61 atggttgcaa gcgaatcaac ttgaatttga agagaagaat atttttgccg aaccgttaac
      121 agaaagtgag atcaaaaaga tcttggcagc gactgaagga ggagttcaag atattatttc
      181 cactcggtcg aaaatttacg aaaagctggg catagatttt aacgaattaa ctttaaagca
      241 aatgatcgtc ttattcaaag aatatccttc attattgaga cgacctattt tgattgatga
      301 aaaaagaata ctaatcggat ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat
      361 tcgatgtgtt gccagaaatc tgatctattt acaaaacaat aacatcttaa attcataggg
      421 agggagcctt cgtggaagtg aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg
      481 cgaaacaatg gttagaacac catcaattgc cttatcaatt tgttgactta gaagaacgcg
      541 atctaagtac agaagaagca gaagaattat gcagttttga agaagtgcaa gcagaacagc
      601 tatttgcgac ttggtctgag gcttttcaca acattgcgat tgacctttcc tgtgtaggaa
      661 aaaaacaact gattttttta tgcacacaac ataccaatct cctcagacgt cctttgatta
      721 ttatcgatga agttctattt attggataca atgagcagtt gatggaacag cttatcttaa
      781 ataaatagac tttaactgtt ttaatcaagt cactgagcat ttcatacagt tgtaacagtc
      841 gaacacttag catcaagcgt gttatgtgtt ctttttttga ctttattttt ctactataga
      901 gacatgctca atttatgtgg tcaaaattca aatctaggtg gggatataga gttaaaaata
      961 tattgacatg tgtaaattta catgatatat tcatttctgt tgttttaagt agcaaagcaa
     1021 taaaatagta ttttaaaaaa catataaaat agttgttgac atgttagaaa tgacatgata
     1081 agataactga gttgttaaaa aagaaccact tgtgaagctt gattctatcg aaaaaaactt
     1141 caaaaaaagt tcttgacaaa taacaaatga taagttatga taaatgagtt gctgtaagat
     1201 ttcgaaaaac gaaatcaaaa aaacttttaa aaaagagttg acaattactt gtctctctga
     1261 tatgatataa aagttgctac ggcagccaaa aacgaatcgg ggaacactcc tgatgaagta
     1321 gacctttgaa aactgaacaa agtaagacaa accaaatgtg tagggcgtct tgattcaatt
     1381 caagacaaca aacatttttt aacaagcaag caatatgcta gcaaacaaat tgagcttaac
     1441 aatcg
//
LOCUS       DAHHPA010000221         1444 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.229, whole genome shotgun sequence.
ACCESSION   DAHHPA010000221 DAHHPA010000000
VERSION     DAHHPA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.229"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>1444
                     /locus_tag="NBH55_002900"
     CDS             <1..>1444
                     /locus_tag="NBH55_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCD1510819.1"
                     /translation="KKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGSSRDKNYLS
                     SMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTEVGV
                     EVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQSPD
                     KVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWDNSFGKVYS
                     GLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGKLGFNGLVV
                     TDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKGIISEERLR
                     DALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQAITLVKDKQ
                     DIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEKYISPE
                     EELKGLSQEEKIKNIAGIYAAKRPIKDLTEK"
BASE COUNT          524 a          180 c          297 g          443 t
ORIGIN      
        1 aaaaaaccat atcatttaga tgaagatgct atttcttgga ttgaagagac gattgatgct
       61 atgactttag aagaaaaaat aggtcaatta tttgttaata tgggatctag tcgtgataaa
      121 aattacttaa gtagtatggt caatcagtac catattggag ctgtacgata taatccagga
      181 actgctgatg aggtatatga tcaaaactat atcctacaaa cagaaagcaa aattccttta
      241 ttaattgctg ctaatacaga agcaggtggt aacggagctt gtacagatgg taccgaagtt
      301 ggtgtagaag taaaaattgg tgcaacaaat gatgcgaaat atgcttatga attagggaga
      361 gtttctggaa ttgaagcatc ggctattgga tgtaactgga gttttgctcc tattgtagat
      421 attaatagaa attggagaaa tcctatcatt tctagtcgat cttttggaca aagccctgat
      481 aaagttttag aacttagttt agcttatatg aaaggtattc aagagagtgg aattttgcct
      541 gcagctaaac atttccctgg tgacggtatt gacgaacgag atcaacatct atcttttagt
      601 attaatagtt taagtacaga agaatgggat aattcttttg gaaaagtata ctcaggctta
      661 atagaagctg gattaccttc aattatggca ggtcatatcc atatgccagc ttattcaaaa
      721 cattttaatc ttgatctgaa agatgaagat ttacttcctg caactttatc aaaagaatta
      781 atcactgact tacttagagg aaaactcggg tttaatggat tagtagttac tgatgctagt
      841 catatgttag gaatgactgg ggcaatgaaa cggtcagaat tattgcccac ttctattgcg
      901 gctggatgtg atctattcct attttttaat gatccagatg aagactttaa ttatatgatg
      961 gaaggttatt acaaaggaat tatttcagaa gagcgtttac gtgatgcatt aagaagaatt
     1021 ttaggcttaa aggctaaact aggtttgcat aaaaaagaaa agaataaaat tttgttaccg
     1081 aaacaagaag ctatggaaaa aattggttta attgaaaata aatcaatttt taaagaggta
     1141 gctgatcaag caattacact agttaaagat aaacaagata tttggcctat tgtaccagaa
     1201 aaatataaaa ggattttact ggtagatgta catggggtaa aaggtgggtt tggtgaagta
     1261 gttgctggaa atagacctca ggccattgat ttattgaaac agattttgtc agcaaaagga
     1321 tttgaagttg aaaaatacat ttcacctgaa gaagaattga aaggattatc gcaagaagaa
     1381 aaaattaaaa atatagctgg aatatatgct gctaagagac caatcaaaga tttaactgaa
     1441 aaat
//
LOCUS       DAHHPA010000222         1383 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.231, whole genome shotgun sequence.
ACCESSION   DAHHPA010000222 DAHHPA010000000
VERSION     DAHHPA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.231"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(30..437)
                     /locus_tag="NBH55_002901"
     CDS             complement(30..437)
                     /locus_tag="NBH55_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510820.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(456..1274)
                     /locus_tag="NBH55_002902"
     CDS             complement(456..1274)
                     /locus_tag="NBH55_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCD1510821.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          408 a          230 c          194 g          551 t
ORIGIN      
        1 tactttaatt gaataccgca tgaaaaaaat tattttggta atttttcaaa ctcttcttgt
       61 atttcttctc ttatgtgttc ttcttttgct actcctagtc tatgctggat atatgagtct
      121 gctaatgtat taacaatttt ttcgaaagaa attctccctt tttcactttg taactccgtc
      181 atatagtctt gtaaaattga gagtttgcta tttaatgccg gagagatctt tttaggaact
      241 gataccttgt ttcttttatc aggaattgaa actggtcggc ctcttttttt caatgtaata
      301 tttcttgtgt tactcaatgc actattgcta aagttatatt caccgcttat tttcacatct
      361 tggcgtttgt tattgggtaa taatggctca aattcaccat tctccatcat tttaggaata
      421 tcaaaatctt taggcattac ttatgcaact ccttattatt tatttaaaaa ctcttgtcta
      481 gcatctagtt ctgccaaaat atttataaat acttggtgtg ctttataatc ccattgatca
      541 aaatttccat ttttgtattt tcttaaatgt ataccaattt tagaatacct tctcaatctt
      601 ttttgataat gaatgagatt tttggatacc acggttccgt atttttcaat tgcttcttct
      661 aggatttctt catcaaaatc atcatttggt tctaccatac aaggtacaat atctataatt
      721 tgcaaatcaa gatcgtatct atcgaccatg aatttttgat aatttacata tttgttgact
      781 ccctctaagg attcttcagc tgtttgaaat gaaattatcg aatagtctga agctgccata
      841 gcattatcgg aaaattcgct aatggttggt ggtacatcaa taaatatttc ttgataattg
      901 ttctttattg gaagaagtaa ttttttcaag acagtaactt ggtctaattc attttcatgt
      961 ttattagcaa aagcggagaa tgatcgaaaa ctagtgtcac atgccagaag gtcaagattt
     1021 tcatttacct taataatact acttgctaat tctccatttg cgacaccatt tgttatagtg
     1081 gttttgatat cggttatacc gaaagttctt gctatgactt gtgttaagtt cgcttgaggg
     1141 tctaaatcaa taataagcgt ttttctattt cttatcattg cattgtcata agcaagcatg
     1201 ctgactatct tagttttacc tactcctcct ttaaaattgc caactgtaaa tgtagtccct
     1261 tgaaaagtat ccatgtaaat cctcccaatc tttaattgat aattaaagta taacttatat
     1321 atcggaaatg gtcaacatta aaagtataac ttaaataata atatctaaaa acgtttggat
     1381 ata
//
LOCUS       DAHHPA010000223         1374 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.232, whole genome shotgun sequence.
ACCESSION   DAHHPA010000223 DAHHPA010000000
VERSION     DAHHPA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.232"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            192..446
                     /locus_tag="NBH55_002903"
     CDS             192..446
                     /locus_tag="NBH55_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD1510822.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="NBH55_002904"
     CDS             447..764
                     /locus_tag="NBH55_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCD1510823.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          267 g          418 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaagaca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccttagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacttccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tata
//
LOCUS       DAHHPA010000224         1368 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.233, whole genome shotgun sequence.
ACCESSION   DAHHPA010000224 DAHHPA010000000
VERSION     DAHHPA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.233"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            220..1251
                     /locus_tag="NBH55_002905"
     CDS             220..1251
                     /locus_tag="NBH55_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCD1510824.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTETDQLQNQVLLDNFVEIMK
                     EDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKAEELEKTVVVYEELESYGI
                     DADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEKAIAAKLANEQRETFNELP
                     TVSTENWLE"
BASE COUNT          572 a          188 c          200 g          408 t
ORIGIN      
        1 acaacgtgta aatttgcgat taaatcaaca ttataaaatt attatttaaa gtataagtta
       61 tacattattt gtatttaaag tataacttat acttattttt ttaatatata acttatacct
      121 taaatactga aagtgtttta ttcaccctta atacatggta tagttcactt gttgaaaatc
      181 ctgaatatat acaagtttct aagaagaagg agaaatttaa tgtctacaga aaatcaatct
      241 caaaaaatga taattaaaaa ttggggatat aaagcaacga atgtttatat tgaaaaatat
      301 tataaatttc ctagtttttt aattcagaat tctaaatact ctaaaatgaa agacagctca
      361 aaaatagcct atatgctttt taaaaatgaa tttcgtagag ccttacatga aaaatggatc
      421 gacgatgatg gtctattata tttggaattt acacaagcag acctaatgga caaattaaat
      481 tgctaccaag gcaaagtaaa aaccatttta aacgagttag aaacatataa tttgatttca
      541 gtcgtaaaag gagaatttaa cccaaaaacg aaaaaaaatg aaaaaaataa atactattta
      601 cttcaacctg aaataagtgc cgatgatctt tttattcaaa attctttcga tgacgattta
      661 agcgaaacta ttgataaatc aacctcttct cggaatgcgg aaatcgcacg tcgagaaaaa
      721 acagctgaaa cccttgataa cactggaaat gcggaaatcg cacaagatta ttatattaat
      781 aaatctttag acactaaaag acacttaaaa gacactgaaa cagaccaatt acaaaatcaa
      841 gttttgctag ataacttcgt tgaaatcatg aaagaagaca gcataaacac atttgtacca
      901 gaaaaggtat taagcttgat aaaaacattt tctaacactt acgaagaagc ccaacaaacc
      961 gtcaccacaa tacacaacgc aaaacataaa gccgaggaac ttgagaagac ggtagttgtg
     1021 tatgaagaac ttgaatctta cggaattgat gcggataaag gattatatat gacgttgctg
     1081 aaagcttatc aaaaacaaaa gactgagaaa gtaaataatt tgcagaattt gatttttgtg
     1141 tatgtaaaaa actggtttat tgaaaaagct attgcggcaa agcttgcaaa cgaacaaaga
     1201 gaaacattta acgaattacc aacagttagt acggaaaatt ggctagagta gttgataaaa
     1261 agcgaacact ataaaaatga tcaattttaa attagaactt ttttacaatt ttaattttat
     1321 tacaaatgtt acaaataaat agttattatt ccggagtttt tagattaa
//
LOCUS       DAHHPA010000225         1362 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.234, whole genome shotgun sequence.
ACCESSION   DAHHPA010000225 DAHHPA010000000
VERSION     DAHHPA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.234"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            82..>1362
                     /locus_tag="NBH55_002906"
     CDS             82..>1362
                     /locus_tag="NBH55_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCD1510825.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAHHPA010000226         1315 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.235, whole genome shotgun sequence.
ACCESSION   DAHHPA010000226 DAHHPA010000000
VERSION     DAHHPA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.235"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            283..522
                     /locus_tag="NBH55_002907"
     CDS             283..522
                     /locus_tag="NBH55_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510826.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="NBH55_002908"
     CDS             complement(884..1144)
                     /locus_tag="NBH55_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510827.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          428 a          240 c          177 g          470 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtt
//
LOCUS       DAHHPA010000227         1302 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.236, whole genome shotgun sequence.
ACCESSION   DAHHPA010000227 DAHHPA010000000
VERSION     DAHHPA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.236"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            129..1169
                     /locus_tag="NBH55_002909"
     CDS             129..1169
                     /locus_tag="NBH55_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCD1510828.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          530 a          179 c          210 g          383 t
ORIGIN      
        1 ctgattttag gtagtattta attaaaaaac agttttaatg cgtttgcatt ttttatttat
       61 ctttgctatt ttcgaaaaga gtaggtaatt ttacataaat aaaacaacgt gaaagtagag
      121 gatctacaat gactgatttt aaatttttta aggcggatag agtttacaac gaattatttt
      181 atcaatttcc aaaagtcttt attgtttctg acgaatacaa aaaaatgaaa gattcaacta
      241 agattgccta tatgctttta aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg
      301 atgaagaagg taatgtttat tttacttata cgacaaatga actatgtaga gtattaaact
      361 gccaaaaaca aaaagcgata gcaatcaaaa aagagttgga atcctttggt ttattattac
      421 aaaagcagat gggatttaac aaacagttag ggaaaaataa tcctaataga ctatatctag
      481 cagaattaaa agtctcagaa aatgatatct acttactcga aaaatttgat agagagaata
      541 gggaaaacgt tgataaatca gagggtatga aaatcatacc caccctcgac gaaaaatcag
      601 acgctgaatc ccttggggct caagagggta tgaaaatcat accgtgccaa aacgttgata
      661 aatcagaggg tatgaaaatc ataccagaac ttaataataa tatattagac actaatagac
      721 acaatataga cactgaaaaa gaccgcctac aagatcaatt gttgttagac aattttgaga
      781 caattatgac aaacgacagc attgctacgt ttgtccctga acgagtatta aatttgataa
      841 aaacattttc ttcaagttac agtgaagctc aaaaaaccgt ccagactatt cataatgcaa
      901 agaaaaaagc tgaaatagaa agtggtattt cgatagtttt tgaagaactc gatagttatt
      961 atgtcaatgc agaacaagaa ttatacacga cactgttaaa agcctatcaa aaattaaaaa
     1021 ccgaaaaagt cgaaaatatc cagaacctga tttttgtcta tgtaaaaaat tggtttatcg
     1081 aaaaaccaat agctgctaaa gtatcaagtg aaaaacgttt gaattatgaa agctccccaa
     1141 gcactattac gaaagactgg ttagagtgaa actttactga caaagtgatt gttgcttcat
     1201 ggagtaacat taaaattaaa aattttttat tattctaatt atattacgaa tattacaaaa
     1261 taatagctat tttaatagaa tttataagtt aaaatattat ga
//
LOCUS       DAHHPA010000228         1242 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.237, whole genome shotgun sequence.
ACCESSION   DAHHPA010000228 DAHHPA010000000
VERSION     DAHHPA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.237"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            10..483
                     /locus_tag="NBH55_002910"
     CDS             10..483
                     /locus_tag="NBH55_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCD1510829.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            492..719
                     /locus_tag="NBH55_002911"
     CDS             492..719
                     /locus_tag="NBH55_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1510830.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          390 a          196 c          208 g          448 t
ORIGIN      
        1 gggataaaaa tgaaatttaa aacattgcta ttaaaatttg tcgttttagg tataagtgcc
       61 gtatttattt tgttcggcgt acttttttat actcagatcg ctacaagcga aaaaggattc
      121 aattttgatt ggcagaccac attatttgtt tctgtcattt actttgccct tctcctaggg
      181 atcgttattg cttatttctt attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt
      241 tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt tattggtttc
      301 gccttttttg gcttacttcc taaatttatg aaagtagcgg atttagacga tgctccaggt
      361 ttgatgttgg taggaatcgc tgtcgtattc attccttttg cagtatacac attaacaagc
      421 gtgctggaaa aactgcttga acatgcgatt ttacttaaga cagatcatga tttgacagtg
      481 tgaggtgcga catggcaatt atcgttaatt tagatgtagt attagctcaa aaaaaaatga
      541 gtgtgactga attgagtgag aaagtgggca ttacgatggc aaacatctct attctaaaaa
      601 atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa gttttggagt
      661 gtcaaccagg tgatttgttg acctataaaa atgactcgaa tactatacca gaagaataaa
      721 ataaagtaga acaaacagta aataaaaaat aatctgaatg atagatgtgt gagactttta
      781 gaagttattt acgtctatta ttcagatttt ttatttcgat tgaataccgt tttatctata
      841 aataagatat gtactgactc atacaaaggg ggccttttct tctccatgta ctatcacttt
      901 tcctgataca actggtttcc tgttacgttg tagttggatc tgcttttagt ataatacttt
      961 attcattcca ccagactgat agcagtttga gcagtctaaa taatcatgta gtattacagg
     1021 ccattctgtt tcataccttg aagtgcttaa aacttcagct aatggcccat tgtgttaact
     1081 cttactccat tcttttttag taagctccca tatatactgt aaagccttct atatattatg
     1141 tcatgttgct ttttaacatt tactatgtag ttttgataaa atgatttgaa taacagtgat
     1201 ggacaattct tttttatttt taacattatt ttgataataa tg
//
LOCUS       DAHHPA010000229         1238 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.238, whole genome shotgun sequence.
ACCESSION   DAHHPA010000229 DAHHPA010000000
VERSION     DAHHPA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.238"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            2..>1238
                     /locus_tag="NBH55_002912"
     rRNA            2..>1238
                     /locus_tag="NBH55_002912"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          373 a          245 c          362 g          258 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      901 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      961 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
     1021 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
     1081 ctaggatgtt ggcttagaag cagccaccat ttaaagagtg cgtaatagct cactagtcga
     1141 gtgaccctgc gccgaaaatg taccggggct aaacatatta ccgaagctgt ggagtacacc
     1201 tttaggtgta ttggtaggag agcgttctaa gggcgttg
//
LOCUS       DAHHPA010000230         1230 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.239, whole genome shotgun sequence.
ACCESSION   DAHHPA010000230 DAHHPA010000000
VERSION     DAHHPA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.239"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..559
                     /locus_tag="NBH55_002913"
     CDS             <1..559
                     /locus_tag="NBH55_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1510831.1"
                     /translation="GIVLMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIV
                     YLLLFLIKPSNMYVFLVLTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYA
                     VYSFARKIGQALAGGVGGWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGL
                     ILFFVYPLSKEKVLENYNLLNQKHN"
     gene            570..>1230
                     /locus_tag="NBH55_002914"
     CDS             570..>1230
                     /locus_tag="NBH55_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1510832.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YP"
BASE COUNT          430 a          141 c          241 g          418 t
ORIGIN      
        1 gggaatagtt ttaatgaact ttatcaatcc tattttgatg ttggtacttg tttcacctgt
       61 aagtttaatt ttcagtaaaa aatacggtaa aaaagaattg gcgtcagtag ggatgttctt
      121 ctctgctatt gtatatttac ttttattttt gataaagcct agtaatatgt atgtattctt
      181 agttcttact tctgttggat atatggggtt aggtattttt aatacagtca tttgggcgaa
      241 tattactgat gtaatcgatg atcaagaagt gaaatctggc caaagagaag atggaactat
      301 ttatgcagta tattcttttg cccgaaaaat tggacaagct ttagctggcg gtgtcggagg
      361 ttggactctt tctataatag ggtatgattc gttagcaaaa gtacaaagtg gagaagtttt
      421 acaaaagctg tataactctt caacgttgat tccagcagtt tgtttctttg ttgtaggatt
      481 gattttattt ttcgtatatc cattatctaa agaaaaagta ttagaaaatt ataatttgct
      541 aaatcaaaaa cataactagg agaaaaaaca tgaacagttg gatattttgt attgattcag
      601 atggttgtgt catggataca atgacttata aacacaatct ctttttcggg ccaattgctg
      661 cagagatttt tgatgttcat catagggaaa aattcctaca ggattggaat aatattaatt
      721 tatattcaga gactcgtgga ataaacagat ttttaggttt agttaaaaca ttagaaaaaa
      781 atgatgtaac atcgattaat cgattaaagc actgggtatc tctaacaaaa gaattgtcta
      841 atccatcttt gaagagggaa atagaatatt ctcagtcaga ggatttatta aaagctttaa
      901 aatggtcgga agaagtcaat caacagatta gtcaagcaaa aggattagat aaaccctttt
      961 taagtgctag agatactata aaagctctga aaaaatatgg gaagataatt attgtaagct
     1021 cagctaataa ggaagctgtt caagaagaat gggaaagaca tgaattatta tcattagttg
     1081 atgaactgtg ttgtcaagat aaaggaaaaa aagaagacat tattcgaagt gtaattgaaa
     1141 atggttgtga tttggataag atattaatga ttggtgattc tcctggtgat ctagaagctg
     1201 caaataaaaa taaagttttc ttttatccta
//
LOCUS       DAHHPA010000231         1217 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.240, whole genome shotgun sequence.
ACCESSION   DAHHPA010000231 DAHHPA010000000
VERSION     DAHHPA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.240"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            100..654
                     /locus_tag="NBH55_002915"
     CDS             100..654
                     /locus_tag="NBH55_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1510833.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            908..>1217
                     /locus_tag="NBH55_002916"
     CDS             908..>1217
                     /locus_tag="NBH55_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510834.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT          495 a          123 c          219 g          380 t
ORIGIN      
        1 ttttcttcac ttttaacaga gtatatatat aagttcatga ttatggtaaa atatatatat
       61 aatcatgaac tcagaatatt tttaagaagg gaaacaatag tgagctataa tgtacaaccg
      121 ttaaggacaa atgaagagat acaagatttt cagttttggc taagaagaac aagtaatcca
      181 gaaagagata gttttttatt tttgtttgga atcaataatg ggttacgtat gtcagatatt
      241 ataggattaa aagttggaga catacgaggt aaaagtaagc ctattattgt tgaacataaa
      301 acaggtaagc gaaaaccgat ttttatagat aatttgagag aagagatttt attatatact
      361 gaaggtaaag aagaaaatga ttggttattt ccaagtcggc aacaaggtcg acatataaca
      421 agggatcgtg tctatcagat atatgcagat atagcagaaa aattaggaag agatgatata
      481 ggaacacata cattaagaaa aacttttggg tatcactact acaagaaaac tagagacatt
      541 gctaccttaa tgtttatttt taatcatagc tcgcaagcaa ttacaaaaag gtacattggt
      601 ataactgaag atgaaatagg ggcatcgtta cgtggtttta aattgggagg ctaattgaga
      661 tattaggata tattttttgt gtataatgaa aacttagaag gtgagattaa tgaagataac
      721 tgaattttcg gaagtattta aaacgtttag taacatttac aatagaatac cagagataga
      781 acaaaatgtt gtagttgata atgattatta tatagagaat gattttaata aaccaagaaa
      841 atatgacaac ctatacaatt ttatacacaa tagaacaaca ataaataaca acagtaacac
      901 tcgagttttg attaattcag catattttca tattaaatat ttaaagaaat atgagtatga
      961 agaagttgta ttagattatg ggctaaatag acagtcattt ttaatttatg caataaataa
     1021 cggaaatttg ccaagcaata aaaagaacat aattattgaa acatatgcct atctggaggg
     1081 gggatctaaa aaactaatta agtcatgtaa tatagagtat caattatcag taggagaaat
     1141 taaatgtgtt ttcgaagaag atttagaact tgacttaaaa aaaatttttg ccagcattca
     1201 tgattatata ggtttga
//
LOCUS       DAHHPA010000232         1198 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.241, whole genome shotgun sequence.
ACCESSION   DAHHPA010000232 DAHHPA010000000
VERSION     DAHHPA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.241"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(41..385)
                     /locus_tag="NBH55_002917"
     CDS             complement(41..385)
                     /locus_tag="NBH55_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1510835.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(411..578)
                     /locus_tag="NBH55_002918"
     CDS             complement(411..578)
                     /locus_tag="NBH55_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD1510836.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            complement(774..980)
                     /locus_tag="NBH55_002919"
     CDS             complement(774..980)
                     /locus_tag="NBH55_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510837.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
BASE COUNT          325 a          278 c          184 g          411 t
ORIGIN      
        1 tgtatcatac tctctgccat tatcattgat gatcatgcac ttaccacctt tcacttgcat
       61 cgaagttagc aaagctttct atcttcttct cttgtttatc taatgctgta agatatctgc
      121 catgaacttc attatgatgt tcaacacata aacaaataag attatctaaa tctaaagcta
      181 agtcaggtct atccttgact tcctttatat gatgaacgtt ctctactcta tgatacttac
      241 ctagtcttct acactcttgg cattcatagt gatctcgttt catcgctttc tctctaagcc
      301 tgcgccattt aggagactga tagaacttaa ccaaacgatc ttctcttgtc aactgtaata
      361 gccatctata gaattcctcg gtcatgttcc gtctcctttc gcaatcttat ttaatgctta
      421 gctattcttt tgccatacaa tggaataact tcattgtttt cctttcgttt atatgtatcg
      481 ctctttattg gtcttctata ctcatatact ttttcgccat tacctttatg aacagtgatt
      541 acttcatact tctgttctaa gtattgtggt ctatacattg ttgtttcctc ctttgtgcaa
      601 aaataaaaag accactcaaa gagtgatcta atatgtaata gtaacttgca cgatgcacaa
      661 aacgcgtacg aaattgcgca cccctatatt tttaaaccgc cgatgcctcg gttgcttaaa
      721 gtcgctggag tgggattgca ccactcacga gaacttacca ggctctcacg aggctactcg
      781 ccatttaccg ctgcgtcttc tacttccgcc acagtgaccg aagcttggtg gtgtacagat
      841 agcacactta ctacattgcc gtacgtagca ccgtatagct tcttaacgat cttttttcgg
      901 tagtcgtaac cgtcataggc atttaatgtc gctggcaagg aatcgaacct tgcatggttg
      961 ccgaagcatt gacctagcac acatgcttag cgtctaccct ttccgccaca gtgacactat
     1021 aaaattattc ttggctgcta ctatttttta ttttgcccat ttttaaatcc aatcatatag
     1081 acattaagac agagcgcaaa aattgaaatt attaatggaa tcatttctct tcacccacct
     1141 ttagttatcg tgtgaataat taaaaaacaa tagacagcaa caaaataaca ttgctttg
//
LOCUS       DAHHPA010000233         1179 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.242, whole genome shotgun sequence.
ACCESSION   DAHHPA010000233 DAHHPA010000000
VERSION     DAHHPA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.242"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(431..979)
                     /gene="aac(6')"
                     /locus_tag="NBH55_002920"
     CDS             complement(431..979)
                     /gene="aac(6')"
                     /locus_tag="NBH55_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCD1510838.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          361 a          238 c          177 g          403 t
ORIGIN      
        1 aatgattttt tcgtgaggtt tcaaggaatt tttcagaaaa aatagctttg tacaaggttt
       61 tccatggtct tccaaaagcc attaaataat tttcgttata ttgtctatta ttgccataca
      121 aagataaaaa agaataaaca aaaagaaact gtgacaaaag tcttgtcaca gtcccttatc
      181 tgaataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt
      241 agaaagccat tttcacaaaa tttttcttac tactcgaaac taagcgactt tttcacaacc
      301 tctggtacac ggtgttccaa aagtagcttc ttcggaaata agttggaatt tcacaaaaat
      361 tcaagaagta attttcgtaa attccttctt atttcttgaa gctgattact tctgtcacaa
      421 cctcttttta ttattgagaa tttggtcgag gaataatcgt ttttgccatc caaatatccg
      481 gtttgtccca gccatttgca tttggtaaga cacctacgat tttataacct aatttttcat
      541 agaattcata cggatgttca cgaaggttct ggatggaagc cactttatca aatgtatgct
      601 catacaggtc cgtttgactt aacgttgttc catggtctaa atcatccgta cctaaataaa
      661 tcgtgattcc tcctctggaa gctacttctt tttctaagta attgactaat cgagtaccta
      721 tttggttctt tcgtcgggag ctttctacaa ctaatggatg caattcccaa cctgtgatac
      781 cgtattgagg gattgcacca ataaatccta ctaactcatc ttggtctacc gctgctaccg
      841 cgattcgttc tggattcatc atttcttcta cttcttctgc cgagctgtct ccatattctt
      901 ccggccaagt cagtctcagt aaatcagaaa gctgatcttt caatactgga ttattacggt
      961 caaattcact gattatcata cgtcttcccc cttgttcttg cttattctaa cagtcctttt
     1021 gaaaaaacga aatgttctcg ctcaaaaatc tttagctacc aatcagtatc tctattcttt
     1081 cattatttat cttacttata aaataaaaaa attatacaga aacactttat tcaaaatagg
     1141 aaagatcagg aaagccatca attggaggaa aaaactctg
//
LOCUS       DAHHPA010000234         1168 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.243, whole genome shotgun sequence.
ACCESSION   DAHHPA010000234 DAHHPA010000000
VERSION     DAHHPA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.243"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..247
                     /locus_tag="NBH55_002921"
     CDS             <1..247
                     /locus_tag="NBH55_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510839.1"
                     /translation="LNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTNLNPNQMCEKL
                     NTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            complement(244..396)
                     /locus_tag="NBH55_002922"
     CDS             complement(244..396)
                     /locus_tag="NBH55_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510840.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(705..1118)
                     /locus_tag="NBH55_002923"
     CDS             complement(705..1118)
                     /locus_tag="NBH55_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCD1510841.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
BASE COUNT          366 a          237 c          182 g          383 t
ORIGIN      
        1 tcttaataac ccagggcaaa agtatgataa agtatttgaa ataataaaag aatttggggc
       61 atatataaag ctacaaaaat ctttttggtt agttaaaact aatttgaatc caaatcaaat
      121 gtgtgaaaaa ttaaatacag tacttgataa taacgattcc ttattcatct gtgaactgca
      181 aaaaaattat caaggtagag ctacagagga aaattggaaa ttcattaacg aacatatttt
      241 ctcttagtaa ggattagata ttttttctct gtttaaacaa ttgcaaatac cgtcaatatc
      301 tgaattagaa acacttacct tttgctcctt gctactacca atagcttgga gcaattcttg
      361 tatttcttct ggagtgcctt ctactgataa tttcattttt cttccctcca atacataaat
      421 taatagacag cagcagttga aagacgacaa gaacatgttt agttttgtga gtgctgctgt
      481 ctaatcaaaa taaacagcaa ccgatgaaga atttaggagg agttgaattc acatcctttt
      541 cttcataatt agttgctgtc tatctaagaa gaagtttaaa acgatgaggg agattgcctc
      601 ccttcgttta ttttgtcgat cctgtttcct aatctttcga cactaccata ataacactgg
      661 taaatgtcta aaaaccgcca tcattccgcc aaaaaaccgc caaattattt ataagcaatt
      721 atttttccgt gtttatatgc ttctgcaaac tctattagag cttccgactt catccgttgt
      781 atacttcttt ctgaataacc cacttcacgg ctaattctgt agtttgagaa gctatctggc
      841 acacagaagc tgtagtagag tatctgacga ctaatcagac taagagccat caaagccgct
      901 agaatcgcat ctctctccgt ttctatatcc atcatctgaa taatcgcgtc ttctgcctta
      961 ttgccatgct tcggtgcctt cggcatatcc gttataatcg gcgacttaat atctatcaaa
     1021 gagcgacctg ccatccgctc caaacgccga aagttcttca gcacatctct cgcattacat
     1081 cttgtctgtt tgaaatctac ctctcgtaac aattgcatca agtcaaaccg ctcctttatg
     1141 tgatataata aatgtgttgg atttattg
//
LOCUS       DAHHPA010000235         1164 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.244, whole genome shotgun sequence.
ACCESSION   DAHHPA010000235 DAHHPA010000000
VERSION     DAHHPA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.244"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..643
                     /locus_tag="NBH55_002924"
     CDS             <1..643
                     /locus_tag="NBH55_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD1510842.1"
                     /translation="KNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRV
                     KEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSP
                     SRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQN
                     ISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            633..>1164
                     /locus_tag="NBH55_002925"
     CDS             633..>1164
                     /locus_tag="NBH55_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1510843.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKD"
BASE COUNT          440 a          145 c          219 g          360 t
ORIGIN      
        1 aaaaaatacc atgattaata catggaataa cattaaggac tctttctgga atattgttac
       61 tggaattgtt aattctgctg aaaatgcatg gacaaatcta aaaaatggtg tttcaaatgc
      121 tattaatcgg gtaaaagaaa tctttaattc gttaagggaa gtcaatttat ttgaaattgg
      181 caagaacatc attgatggac ttataaatgg tgtaaaagaa aagtggaatg ctttgaaaga
      241 tactatcaaa ggtattgcag gtaatatcaa aaattggatt aaaggagctt tagatattca
      301 ttctccatca agatggatgc gtgatatggt tggtaaaaat attgttcaag gtattatcgt
      361 tggtattgat aaagagcaaa gtaagttaga tcaaaccatg acaaatttag taaaaacacc
      421 gtctgttcaa cctgtagtaa atggaataag tcacctacca attacacaaa caaagcaaaa
      481 tatatctgct agtgaaacaa aagaaattca tttacattta aatgtttatg gagatttacc
      541 tgattcaatg gttcggaaga ttaccacaaa aattaaaaca gaattgacaa gacaaatgaa
      601 acgagatgct ggtgcagtag gaggaacatt atatgcaact taagagagga caatttttta
      661 ttaatcaaca ttattcttct gaatttaatg tgtatattca aaacaggcct gcttctgttt
      721 cagctagtcg agtaattgaa ttgagagagc gagaaggaaa tgattctatc attattgata
      781 aaggctatta cagaaatgtg actagaaaga ttgagtgtta ctataaagca ccttcaattg
      841 atgtggtgca ggagtgggaa gatcgaatca ctgaatggtt agatatgtcg tcttatagtg
      901 attttattct ttactatgat gaacaatata tttatcaagc tgtagtaata gaaggacctg
      961 aatttaaagg aacaagaaaa acaggaaata ttgtcccttt tgaatttaca gtgagcattc
     1021 gaccatttaa ggaaaattac aatggtagat ttactattcg acaaacagaa acttttgaaa
     1081 tttataaccc agagaagtat tcttcaaaac cgcttattaa attgagtggt tctggagatg
     1141 cttcttttta tattaataag gaca
//
LOCUS       DAHHPA010000236         1164 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.245, whole genome shotgun sequence.
ACCESSION   DAHHPA010000236 DAHHPA010000000
VERSION     DAHHPA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.245"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..126
                     /locus_tag="NBH55_002926"
     CDS             <1..126
                     /locus_tag="NBH55_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCD1510844.1"
                     /translation="RDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            131..1078
                     /locus_tag="NBH55_002927"
     CDS             131..1078
                     /locus_tag="NBH55_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCD1510845.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          375 a          171 c          247 g          371 t
ORIGIN      
        1 agagattatc taattttaga tattgatagt gaagaagatt ttaaatggtt agaatttttg
       61 tttcctaaat ttttagaaga ttcttcggag cttcaagata tctataatta tttatatcgg
      121 aggtaaaagg atgagctgtt atcttatcac cggtggggct ggcttcattg gttctacctt
      181 agccaatcat ttaggagaag aacatgtggt aatcgtcgtc gatgacttat caatgggaaa
      241 agtagaaaat cttgatatga cgaaaaatat cacttttatt gagggagatg tcgctgagaa
      301 atccttgatg gaagagatca tgaaagctaa taaatttgat tatatctttc atttagcagc
      361 agttgctagt gtggcagatt cagttgcccg accagtagaa acacatcgtg tgaattttga
      421 aagtgtatta atgattttag aattgatccg caagtatcaa cctgatttaa aacggatcgt
      481 gtttagttct tcagcggctg tttatgggga tgaaccgact ttaccaaaaa aagaagagtc
      541 cgtgattcgc ccattgacgc cttatgcggt tgataaattc gctgcagaac aatatgtgct
      601 agattattgt catttatatg atgtacccgg tagcgcagtt cgttttttca atgtctatgg
      661 tccaaatcaa aatccgaatt ctccatacag tggtgtgatt tctattctcg tagatcgcta
      721 taagaaacaa atcgtaggag aagcaacttc atttacatta tttggtgatg gaagccagtc
      781 tagagacttt gtttatatag atgatgtaat ccaagcatta cttttagttg cgaatgagga
      841 agctgcactg gggaaacaat tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt
      901 gattcatgca atcaatgaaa ttctcggtgt agaattgaca cttgaataca aagaagagcg
      961 ttcgggtgat atccatgact ctctagcaga tattactaag atccagtcaa ttggctatca
     1021 accgaaatat ggtattttaa gtggaatgac taattatttg aaaactgaaa tcaattaaaa
     1081 catactaaga taagattaga gggtaaattc aggctctttg tcaataagga ctgatgagtt
     1141 ttgtaaaatc aaatctggga taga
//
LOCUS       DAHHPA010000237         1064 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.246, whole genome shotgun sequence.
ACCESSION   DAHHPA010000237 DAHHPA010000000
VERSION     DAHHPA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.246"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(38..994)
                     /locus_tag="NBH55_002928"
     CDS             complement(38..994)
                     /locus_tag="NBH55_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510846.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caac
//
LOCUS       DAHHPA010000238         1053 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.247, whole genome shotgun sequence.
ACCESSION   DAHHPA010000238 DAHHPA010000000
VERSION     DAHHPA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.247"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="NBH55_002929"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="NBH55_002929"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="NBH55_002930"
     tRNA            203..276
                     /locus_tag="NBH55_002930"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="NBH55_002931"
     tRNA            316..405
                     /locus_tag="NBH55_002931"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="NBH55_002932"
     tRNA            420..491
                     /locus_tag="NBH55_002932"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="NBH55_002933"
     tRNA            507..579
                     /locus_tag="NBH55_002933"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="NBH55_002934"
     tRNA            589..661
                     /locus_tag="NBH55_002934"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="NBH55_002935"
     tRNA            697..772
                     /locus_tag="NBH55_002935"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="NBH55_002936"
     tRNA            774..854
                     /locus_tag="NBH55_002936"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="NBH55_002937"
     tRNA            861..934
                     /locus_tag="NBH55_002937"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="NBH55_002938"
     tRNA            937..1009
                     /locus_tag="NBH55_002938"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
BASE COUNT          250 a          234 c          285 g          284 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctt
//
LOCUS       DAHHPA010000239         1035 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.248, whole genome shotgun sequence.
ACCESSION   DAHHPA010000239 DAHHPA010000000
VERSION     DAHHPA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.248"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..>1035)
                     /locus_tag="NBH55_002939"
     rRNA            complement(<1..>1035)
                     /locus_tag="NBH55_002939"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAHHPA010000240          991 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.249, whole genome shotgun sequence.
ACCESSION   DAHHPA010000240 DAHHPA010000000
VERSION     DAHHPA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.249"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            158..>991
                     /locus_tag="NBH55_002940"
     CDS             158..>991
                     /locus_tag="NBH55_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCD1510847.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QR"
BASE COUNT          298 a          195 c          186 g          312 t
ORIGIN      
        1 aaaaaatcct caacgacgtt tagtttatga aaacaggctc tatacttttt gatgttggtg
       61 aacttcacca acatcttttt tgtataaaaa aatatagaaa agacaccctg aaatccttta
      121 gaattaattt ggctaccaat tcataggagg attcaagatg tctcatactc agcttatcaa
      181 agatacccta aatattctag acctaaatat ctatttcgag gaaaattgtt taactaaaga
      241 aaaatacaaa gggcaaattt gcttgattta caaaggaact cttctgtaca agccagaaga
      301 gtgttcccat tgtctttgtg tcgtcccctc taggatcatt cgttggggga cgacaaccgt
      361 tcggttgtta ctgaatgatg tgtctgagta tcgcacttat ctggagctga agaaacaacg
      421 atttaaatgt aagtcttgcc aaagaacatt cgtagcggat acctctgttg ctgaaaaaca
      481 ctgtttcatt agtcaaaaag ttcgttggtc tgtcattgct cgcttaaaag agaatacttc
      541 catgacggaa atcgctcgtc agaaaaatat ttccacttct tcggtctatc gtgtcatgaa
      601 gcgattctac cgcccattga atcctttcaa acagactttg ccaaaggtct tatgctttga
      661 tgaattcaaa tcggttcgaa gtgttgccag tgcaatgagt tttatcatga tggacggaca
      721 aagccatgca ttattggata ttgtcgaaaa tcgacgcttg ccttatttag aacgctattt
      781 ttctcggttt tcattagcga ctcgtgaagc cgttcaattg attgttattg atatgtatgc
      841 gccttatgtt tcgttggtga aaaagctttt cccgaatgct cagttgatca tcgatcgttt
      901 ccacatcgtc caacatattg gcagaacttt cttgaatcat cgagtgaaag aaaccacggt
      961 acgacttaaa ggaaattccc actctcaacg a
//
LOCUS       DAHHPA010000241          982 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.250, whole genome shotgun sequence.
ACCESSION   DAHHPA010000241 DAHHPA010000000
VERSION     DAHHPA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.250"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..125)
                     /locus_tag="NBH55_002941"
     CDS             complement(<1..125)
                     /locus_tag="NBH55_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCD1510848.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="NBH55_002942"
     CDS             complement(143..463)
                     /locus_tag="NBH55_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1510849.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            762..>982
                     /locus_tag="NBH55_002943"
     CDS             762..>982
                     /locus_tag="NBH55_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCD1510850.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESG"
BASE COUNT          343 a          168 c          145 g          326 t
ORIGIN      
        1 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
       61 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
      241 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
      301 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
      361 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
      421 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
      481 taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta
      541 atgagctata ctatatcttg taaacagatt taacaactaa aaagacaaca aaaaacacta
      601 ttgatatgta aatgcagacc gccaagaaag ctttaaaatc aatgttttta tgtcttattt
      661 aattatgctc ttagtataga gtattactca gatactgtca attgaattta gaaaaaagtt
      721 gttaaatttg tttacgaata taaaagaaag gaatgagaaa tatgaacaca ccgcaaattt
      781 ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa tgatgaacca tattttgtag
      841 gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa agatgctttg tcgcggcatg
      901 tagatttgga agataagatg gggtcgcgaa tcacgacctc aggtcaatca agagagatga
      961 caatcatcaa cgaatctggt tt
//
LOCUS       DAHHPA010000242          977 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.251, whole genome shotgun sequence.
ACCESSION   DAHHPA010000242 DAHHPA010000000
VERSION     DAHHPA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.251"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            346..977
                     /locus_tag="NBH55_002944"
                     /pseudo
     CDS             346..977
                     /locus_tag="NBH55_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          332 a          180 c          178 g          287 t
ORIGIN      
        1 tagttatata tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg
       61 aaaatggtta gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct
      121 tagacacaca catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag
      181 agcgtttagg ccactcttct attcaaatca ttatggatct atatattcat attacagaca
      241 aaagaaaaga agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact
      301 gatgaatcaa aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg
      361 aaagagttga aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg
      421 gtggatgaat ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat
      481 ggcgagactg gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac
      541 tattttagtc gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc
      601 gcctacttcc aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt
      661 catatcatca aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga
      721 ttacaccagt tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt
      781 ctaaaaaacc gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca
      841 cctaaatacc cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg
      901 cctttaaagg acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa
      961 tcagacttat tttttac
//
LOCUS       DAHHPA010000243          972 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.252, whole genome shotgun sequence.
ACCESSION   DAHHPA010000243 DAHHPA010000000
VERSION     DAHHPA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.252"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..793
                     /locus_tag="NBH55_002945"
     CDS             <1..793
                     /locus_tag="NBH55_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCD1510851.1"
                     /translation="NLWLAKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQN
                     ATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGN
                     IGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLG
                     ERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVS
                     LDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
     gene            826..>972
                     /locus_tag="NBH55_002946"
     CDS             826..>972
                     /locus_tag="NBH55_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCD1510852.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSY"
BASE COUNT          382 a          110 c          179 g          301 t
ORIGIN      
        1 taatttatgg ttagccaaaa agaatggaat cagacagaga attgctcata gccatgcaac
       61 tgaagatgga aaatctcaaa atattgtaat gaatgtattg cgtaaatttt taaccaaatt
      121 gttgttacaa aatgcaacag actaccttgc cgttagtcaa gaagctggtg aaagtctgtt
      181 tcaaaaccat ccctttgaga tagttcataa tggtattgat ttagagcttt atagtaaaaa
      241 ttcagaagct aaggttaata agagaaaaga attggatatc tcaatgagta cttttgttgt
      301 gggaaacatt ggacgattag agaaacaaaa aaatcaatct tatttgttag agattttttt
      361 agaaatacta aaaatgaatt ctgattctta cttgatttta gtaggtgaag ggtcacttag
      421 aaataaactg aaagccaaag cagtagaatt ggatatagag aaaagagtaa aatttttagg
      481 agaaagaaac gatattcccg agttattatc tgtgatggat gtttttgtaa tgccttctct
      541 ttatgaaggt ctgccaatgg ttggattaga agcacaagca gcaaaattaa aattagtctt
      601 agcagatact atttccaaag atacaaagtt aacaaataat gtggattttg tatcattaga
      661 tgaaacacca caagaatggg caaaaaaagt aataaaagag ccgtatgcta ataaaataac
      721 acaagaacta aagagttatg atgtttcgta cactactgaa caaatggaga gtatatatag
      781 tagagatcat taaaaatgtt caagatattt taggagtgat tatttttgaa aatatgttat
      841 ttgatattgg catacaatga tattggcaac ttaaagagat tggtcaatcg attgaacgaa
      901 aatgcagttt ttattattca tatagataaa aaaactgata tagaaccatt tattgaagca
      961 tttaaatcat at
//
LOCUS       DAHHPA010000244          936 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.253, whole genome shotgun sequence.
ACCESSION   DAHHPA010000244 DAHHPA010000000
VERSION     DAHHPA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.253"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(62..280)
                     /locus_tag="NBH55_002947"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="NBH55_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="NBH55_002948"
     CDS             complement(546..740)
                     /locus_tag="NBH55_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510853.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..>936)
                     /locus_tag="NBH55_002949"
     CDS             complement(840..>936)
                     /locus_tag="NBH55_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1510854.1"
                     /translation="LKAATINNNVFNASQNPFGGQCIVISDDLPF"
BASE COUNT          320 a          158 c          147 g          311 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaac
//
LOCUS       DAHHPA010000245          925 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.254, whole genome shotgun sequence.
ACCESSION   DAHHPA010000245 DAHHPA010000000
VERSION     DAHHPA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.254"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="NBH55_002950"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="NBH55_002950"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCD1510855.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
BASE COUNT          260 a          169 c          178 g          318 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttc
//
LOCUS       DAHHPA010000246          893 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.255, whole genome shotgun sequence.
ACCESSION   DAHHPA010000246 DAHHPA010000000
VERSION     DAHHPA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.255"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            1..855
                     /locus_tag="NBH55_002951"
                     /pseudo
     CDS             1..855
                     /locus_tag="NBH55_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          371 a           97 c          140 g          285 t
ORIGIN      
        1 attagctttg agccaactga ttcatttcag tatcttagca aagatattga aattgaagat
       61 atttataaaa ttttagctca agagctatct aaaaatgtta ttagtttgcc aaaggttttt
      121 tattttccag ccgaaagaac tggtataaat gtctttaaga acgaactaaa tgaaagtcgc
      181 ttaaaaacat atgatacctt gctgaatact gtgcaattta ctaatttaac taaccaaaaa
      241 aagaaagctg ccctcaagta cgatctgtta aaaaagaata ttgagctgtt aatggagaca
      301 acggataata gttcttatcc aaagccaata tcggattata ttaattatct gaataacatg
      361 aaaaatagat atgaacagaa aaatgtcaat aatatagcat catatattag agatgagatt
      421 attatgggta aatatgaaat tgacgaaaaa gataattctg taagttttag acaaaagttt
      481 ggtaaaaata gatatagaaa agaacgaatt ccttttcacg ttgtgtcttc ttcgattaaa
      541 tctctatatg gattagatta ttacttagaa aattcaagta aagaaggaga ctatcttatt
      601 attgatgagc cagaattgag tttacatcca gaaaatcagg ttaagttagc aaaagtaatt
      661 acagatatag ttgagagtaa aattaagcta attacttcta cccatagtga tttatttgta
      721 cgtgagctta caaatattgt tattgaaaat aaattaaata gtaagaataa taaaaaaaat
      781 aaattgtctg ataaaaatat aggcatatat aattttgtag gaaataatga tataaatgaa
      841 ttagaaaaaa tttcgataaa gtccgtataa ttgtgtaaaa gtaaaaaggc cat
//
LOCUS       DAHHPA010000247          890 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.256, whole genome shotgun sequence.
ACCESSION   DAHHPA010000247 DAHHPA010000000
VERSION     DAHHPA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.256"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..888
                     /locus_tag="NBH55_002952"
     CDS             <1..888
                     /locus_tag="NBH55_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCD1510856.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAHHPA010000248          864 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.257, whole genome shotgun sequence.
ACCESSION   DAHHPA010000248 DAHHPA010000000
VERSION     DAHHPA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.257"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..864
                     /locus_tag="NBH55_002953"
     rRNA            <1..864
                     /locus_tag="NBH55_002953"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          215 a          188 c          272 g          189 t
ORIGIN      
        1 atgcaggtta cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt
       61 gagtgtctgt accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc
      121 gatggaggcg ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa
      181 tcgtggtggg agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt
      241 aacggaggcg cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc
      301 agaagggagc ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga
      361 tccggtggtt ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac
      421 aggcttatct cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc
      481 gtcgcatcct ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca
      541 cgcgagctgg gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga
      601 aatttgagag gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc
      661 agttgttctg ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc
      721 atctaagtgt gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc
      781 ctgagagatg atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact
      841 gatactaatc gatcgaggac ttaa
//
LOCUS       DAHHPA010000249          849 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.258, whole genome shotgun sequence.
ACCESSION   DAHHPA010000249 DAHHPA010000000
VERSION     DAHHPA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.258"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..277
                     /locus_tag="NBH55_002954"
     CDS             <1..277
                     /locus_tag="NBH55_002954"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCD1510857.1"
                     /translation="YEKVQIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDK
                     IILMAYCELNENEVKDHRPKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            479..849
                     /locus_tag="NBH55_002955"
                     /pseudo
     CDS             479..849
                     /locus_tag="NBH55_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          340 a          117 c          162 g          230 t
ORIGIN      
        1 atatgaaaaa gtacaaatag ttgatgttaa taatggaaat agatttgaaa cttatacaat
       61 agctggagag agaggaacag ggatgatttg cttgaatgga gctgcagcta gatgtgtatc
      121 aactggagat aaaataattt taatggcata ttgtgaatta aatgaaaatg aggttaagga
      181 tcatagacct aaggttgttt ttgtcgatga taataataaa gttgaaagag taacatcata
      241 tgaaaaacat ggaaggttat cagatataat tctttaggtt ttgtaatgtt atttatatat
      301 agctagacta ttattgaaat atttattgat tactaattgg atagatgtca ataaagtaga
      361 cacaaaaaga gaagatacag actgtagaca aagtccattt tatgtgaatc ggctacacta
      421 gactagacag aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat
      481 gtcaagacgt caacgaagaa cctattcaaa agaattcaaa caacaaatcg tcaatctcta
      541 tctcgctggt aagcctcgcg cagaaattat tcgagagtat gagcttacgc cttcttcttt
      601 cgataaatgg atgaagcaag cacaatcaac gggctcattc aaaaaaagag acaacttaac
      661 accagaacaa gcagaattga tcgcactaag aaagaaaaat aagcaactcg aaatggagaa
      721 tgatatttta aagcaagcgg cgctgatatt cggacgaaaa gacaagtaat tgatgccaac
      781 aagcataaat attccatatc agcgatgtgc aaaattctaa atatttctcg tcagacctac
      841 tattatcaa
//
LOCUS       DAHHPA010000250          842 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.259, whole genome shotgun sequence.
ACCESSION   DAHHPA010000250 DAHHPA010000000
VERSION     DAHHPA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.259"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(104..>842)
                     /locus_tag="NBH55_002956"
     CDS             complement(104..>842)
                     /locus_tag="NBH55_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCD1510858.1"
                     /translation="VVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVY
                     FSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFT
                     IWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQV
                     LVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVL
                     FNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          325 a          145 c           97 g          275 t
ORIGIN      
        1 ataaataaat aaatctcctt ttgattctcg gtaaaaattt tgataaacat ttaatatatt
       61 aatcaaatgt aaaggagtcc aagcaatgaa agctgtcttc atattaattc tcctttttta
      121 aatacaagta agaaatgctt cccaaataat accagaaaac aattgaaatg acatcgcgct
      181 tattgaaaag tacatgagcc tccaccattt catctataaa aatagatagt ataaaaatta
      241 aaacaatacc aagaaacgca taattcactt tacttctaaa aaatgttttg agtaaactaa
      301 agatatttaa cacatagaaa gcatagatac atataaaagc aattatacca cttgatacaa
      361 gtacttgaag aaatccatta tgcatgtttc catgtgctct ttttagttca ttcacatctg
      421 tagtatcaag atctactttt ttcaaaaatt ttgcatcgct attcccatag aaatcggtct
      481 ctccataacc aaataacaaa gttggtctta taactttaag cgcagctttc caaatcgtaa
      541 aacgaccagc tgaaatttcg gagttatcac tagaatgctg taatattact tctttttctg
      601 gaagtggagt tttatctgat tcatttgact ctataacttc tacttttcta tcaatcgata
      661 caggaattgt tgctaaaaaa tccttagcat aattagtacc aaaagaaaaa tataccaata
      721 gaactaataa tatgcttacc atccatttga tttttacagg tagtacttct ctaatgctct
      781 ttctaaattt tgaattggca aacaacaaaa gaaataatac taaaaaagaa caaattacta
      841 cc
//
LOCUS       DAHHPA010000251          832 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.260, whole genome shotgun sequence.
ACCESSION   DAHHPA010000251 DAHHPA010000000
VERSION     DAHHPA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.260"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            41..721
                     /locus_tag="NBH55_002957"
     CDS             41..721
                     /locus_tag="NBH55_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCD1510859.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRDINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          303 a          136 c          154 g          239 t
ORIGIN      
        1 taagatagaa taatagaaaa agatagcagg aggaataacg atgaatcatt ttaaaggaaa
       61 gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt ataaccttag
      121 ctatcgtgaa gttcaagaaa tcttatatga tcgtgacatt aacgtttctc atacgacgat
      181 ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa agaaaaataa
      241 aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag gaaaatggca
      301 ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt tacgtaaaaa
      361 acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt ttgatgaacc
      421 gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga aactaaaaga
      481 atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata atttgattga
      541 acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac gcactgcctc
      601 taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc gaaaagaagg
      661 cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa tcccagctta
      721 aatcatagat accgtaaggg attttattct ttatttaaaa ctttgcaaca gaaccataat
      781 aagtctctat ttttcaataa ctattgcaaa attatatctt aattaagaaa at
//
LOCUS       DAHHPA010000252          817 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.261, whole genome shotgun sequence.
ACCESSION   DAHHPA010000252 DAHHPA010000000
VERSION     DAHHPA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.261"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..472
                     /locus_tag="NBH55_002958"
     CDS             <1..472
                     /locus_tag="NBH55_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HCD1510860.1"
                     /translation="YNQLNDENKVATYEFASNRLEEQKQENVLDFPKKEKIPTVHNSA
                     SAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSMEPVIRNGQFVFVKEQPDVED
                     GEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYEDRIVSPEQIRIIGKVVF"
     gene            559..>817
                     /locus_tag="NBH55_002959"
     CDS             559..>817
                     /locus_tag="NBH55_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCD1510861.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEI"
BASE COUNT          314 a          111 c          164 g          228 t
ORIGIN      
        1 atataatcag cttaacgacg aaaataaagt tgctacctat gaatttgcaa gtaatcgatt
       61 agaagaacaa aaacaagaaa atgtcctaga ttttcctaaa aaagaaaaaa tcccaactgt
      121 tcacaattcg gcttcggctg ccaatccaac agaacttact tacggtgata cggttgtcga
      181 agaggaagaa tttgagagag ttccaagtgc tgctgacttc gcagtaccta ttataggcga
      241 ttcaatggaa cctgttatca ggaatggaca attcgtattc gttaaagaac aaccggacgt
      301 agaagatggg gaaattgcta ttgtggaatt aggtggtgac ggagttacat gtaaagagat
      361 atataaagat tacgaaaatc aaacaatcat tttacgttct ataaacgact tatacgaaga
      421 tagaattgta tcgccagaac agattagaat tatcggaaaa gttgtatttt aaaaaaattc
      481 ccatgccgga gccgtcaact tcggtaaggg tttatatgat tttgacaatt attttataaa
      541 agaagcgagg cgttttgaat gggagtacta ttgtttttag ttggaatagt tttaataatt
      601 atgggtagta gcaaattatc tgaagctaaa aaattaaaag ataaaacttc tgccttagag
      661 agtaaggtaa aggaatattc tcaagaatta aatgctaggg aaacgtttat atctgaaaaa
      721 gagaaaaaca taagtaaatt gaataaaaac ttgtcaacgc tagaaaacaa aattgatatt
      781 cttaatgctg aaagccagcg gttagacgat gaaatta
//
LOCUS       DAHHPA010000253          811 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.262, whole genome shotgun sequence.
ACCESSION   DAHHPA010000253 DAHHPA010000000
VERSION     DAHHPA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.262"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..672
                     /locus_tag="NBH55_002960"
     CDS             <1..672
                     /locus_tag="NBH55_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1510862.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAHHPA010000254          768 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.263, whole genome shotgun sequence.
ACCESSION   DAHHPA010000254 DAHHPA010000000
VERSION     DAHHPA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.263"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            120..>768
                     /locus_tag="NBH55_002961"
     CDS             120..>768
                     /locus_tag="NBH55_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1510863.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQKK
                     RILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERFRW
                     LRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK"
BASE COUNT          301 a          114 c          121 g          232 t
ORIGIN      
        1 tttttcatgt attcttttat ttttataact agtgtattac ttcaaaattt gatattatat
       61 ggcatgaaca gaattaaaat atcagttaaa ataaaagtta aggagcattg tgtatgacaa
      121 ttaaaactag tctccaaaat caagcttacc aatctattcg ccgacaaatt atctatgctg
      181 acttagaacc tggaaaaaaa gtatcagaaa aaaaattaga agaaacactt tctattggtc
      241 gtacaccaat acgtgaagca ctgattcaac tccgacaaca aggtcttgtt gatacaattc
      301 ctcaaagcgg aaattatatc tcattaattg atttagattc ggccaaaaat gctcgatttg
      361 tcagagaaca tttggaacga caaattatgt tagagtgttg tgccaaaatg aataaccaaa
      421 aaaaacggat tttagaaact ataatttcag aacaagaaaa agctgttagt caaaaaaatg
      481 ctcgtagttt ttttcataat gataatttat tccataaagc ttgttttgaa attgcagagc
      541 gagaagaagt atggcaatgg ttagaaaata acaatacaca cttagaacga tttcgttggc
      601 tacgagtaaa aacggaagga ttaaagtggg aaaaaattat ggaacaacat tatcaactat
      661 ttgaggcatt aacaaaacaa gatccagaag aagctaactt tctaacatcg ttgcatcttc
      721 atttaatgct taatgagcaa aatattgttt tagctactca tccaaaat
//
LOCUS       DAHHPA010000255          761 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.264, whole genome shotgun sequence.
ACCESSION   DAHHPA010000255 DAHHPA010000000
VERSION     DAHHPA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.264"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..>761)
                     /locus_tag="NBH55_002962"
     CDS             complement(<1..>761)
                     /locus_tag="NBH55_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCD1510864.1"
                     /translation="ETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESYPISVPSS
                     YNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNGKFITQHK
                     GGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKKENLVNFD
                     FFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILIAIYDEEA
                     QLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISL"
BASE COUNT          230 a          154 c           94 g          283 t
ORIGIN      
        1 taaactaatt ttcatactat ataaataagc gtctagaggt tcccaaaaaa ttggattctt
       61 aagctgaatc attcctttct ttccttcagc ttcagctaca agttgagcct cttcgtcata
      121 tatcgctatt aagatttttc cggtccattc tgtatcgata ttgaattcta cacttgcatc
      181 caaatcagtt attcttgtat taatagtaat atcacttata tagtctttcg gagttgtata
      241 taacttaaca ggtctatgaa tacctgcata attgaagaaa tcaaaattaa ctaagttttc
      301 cttttttata tccccatttt cctgtactgt ttccttcata atccccacag gtaaagtact
      361 atcgtcaata atattgttta cagcaactgt aattcgatta ttcccgtctt ttacaaaccg
      421 attaatttca gactcaaatg gtgtaaaccc acctttgtgt tgagtaataa actttccatt
      481 caaaaaaact tttgcttcat gtgttgcaga tccaaaacga agaactaatc tttcttctaa
      541 taatctttta ggaagagtca cttctttttc ataccacacc catccaacat gatttctaat
      601 gttctctgtt tcaaataaat cattataaga agagggaact gaaatcggat aactctcttt
      661 ttcatttatt ggttctaaag caagttcttc ctgtaagtca tcatttgtat ctaatttaaa
      721 tctccagaga ccgcttaagt caattacttg cctcgtctct g
//
LOCUS       DAHHPA010000256          728 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.265, whole genome shotgun sequence.
ACCESSION   DAHHPA010000256 DAHHPA010000000
VERSION     DAHHPA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.265"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            104..472
                     /locus_tag="NBH55_002963"
     CDS             104..472
                     /locus_tag="NBH55_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCD1510865.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          261 a          119 c          112 g          236 t
ORIGIN      
        1 aaatatagcg gttacaacat tacttgtgtt ttgttcgttt tttatacata actattgttt
       61 ttctggtaaa ataaatcaaa ccacttaatg gaggcgtttt agaatggaaa aatttcagcc
      121 gatcatcaat cagatcaaag aaaaagaaca tcaacaaaag atcaaagacc tttttgctaa
      181 gataacagaa acgtatcccc agctagacac aactgtaaaa tggaaccaac ctatgtttac
      241 tgaccatggt acctttatta ttgcatttag ttttgctaaa aatcattttt ctatcgcacc
      301 ggaaaaagca gcgattcgtg cccttgaaaa aaatatccaa gaagcaggct acgtatatac
      361 ggataacgtc atcaaagttc cttggaaaag tgtaatcaac tgggaattga ttgaacaatt
      421 gatttcattt aatattgaag ataaaaaagg tcatgataaa ttttggcgat aaagctttat
      481 acgaataaac cttttactgg cgaggaaact attcatcagc ttatacaggt tttgttcaat
      541 cctatccttt ttatcatccc tggttaaaca cttaattgac aaagtatctg ctatttttat
      601 aaggagtctt tcttgtgctg aaaaaaatca ctgattgaat ggctttgaat tttccactta
      661 gtaaaattat tttttcagca attttcctgc aaaacttttg aataaggaaa aaatacgaga
      721 aaatatca
//
LOCUS       DAHHPA010000257          704 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.266, whole genome shotgun sequence.
ACCESSION   DAHHPA010000257 DAHHPA010000000
VERSION     DAHHPA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.266"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(281..478)
                     /locus_tag="NBH55_002964"
     CDS             complement(281..478)
                     /locus_tag="NBH55_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1510866.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          247 a          125 c           95 g          237 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aattttttct accgacataa
      241 acaaaaaaac cacctcatat gaggcggtta acgttcaaca ttattttacg atatttactg
      301 cttgaggacc acgttgtcct tcttcaatat cataagtaac tgcttggcct tcatctaaag
      361 acttaaagcc ttctccttgg attgctgaga aatgagcgaa tacgtcattt ccatcttcac
      421 cagtgatgaa accaaaacct ttatctgagt taaaccattt tactgtaccg ttattcatat
      481 atatttcctc ctgatacgta tataatacgt gttttattgc aattaatatt acttggtaaa
      541 aggagttttt atagtgtgaa tatatcgctc agattacgtt tcaaattaga ttacttattt
      601 actataacac ggatgtaatg ctaacacaag gataatcact cgatatacgg tttaaatatt
      661 attatttgtc aaattaagcg agacaaaact ttccatttac gtaa
//
LOCUS       DAHHPA010000258          700 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.267, whole genome shotgun sequence.
ACCESSION   DAHHPA010000258 DAHHPA010000000
VERSION     DAHHPA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.267"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            155..>700
                     /locus_tag="NBH55_002965"
     CDS             155..>700
                     /locus_tag="NBH55_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCD1510867.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEV"
BASE COUNT          241 a          101 c          115 g          243 t
ORIGIN      
        1 cctaatttgt gtctctcagg gcaattctaa gaacgtttca agagatagcg acactatcgt
       61 tatcgaaaaa ggcgaaaacc tatctcattt tctgctaatg taagttggtt tagcttttgt
      121 taatatgact ttatgaaaat ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc
      181 aagcttgaat aaaggatcct tttttcatat ctatgaaaac gaagattttg aactgtataa
      241 tatcctttca caaaaagagg tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa
      301 atctattttc aagtatgcat tttttgcttc caatgatttt caaagaatgg agattaccga
      361 ctgtatattc gaaaactgtg atttttcagg ctgtatattg acaaaaagca tgttgcatcg
      421 cgtgaaattt gtcaattgta aattaacagg tactaaattg atggaaagtt atattgggaa
      481 cacgttattc gagaattgta aaatggacta tgtaaattta tctggttcaa acataaaaga
      541 aagtaccttt gagcactctg tcttatcaag cgcagatttt gtagactgtt ccctcactaa
      601 aataatgttt tctactaatg atttagaaaa tgctagcttt tttgatactg atttaaaaaa
      661 tatcgattta agtagcaact cctttgaaat gattgaagta
//
LOCUS       DAHHPA010000259          684 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.268, whole genome shotgun sequence.
ACCESSION   DAHHPA010000259 DAHHPA010000000
VERSION     DAHHPA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.268"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            39..>684
                     /locus_tag="NBH55_002966"
     CDS             39..>684
                     /locus_tag="NBH55_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1510868.1"
                     /translation="MKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQKK
                     QVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRW
                     LRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHP"
BASE COUNT          262 a          107 c          118 g          197 t
ORIGIN      
        1 attcaaaaat gaaattttga gagggattaa atatgacaat taaaactagt ctccaaaatc
       61 aagcttatca atctattcgt cagcaaatta tctacgctga cttagaacct ggaaaaaaag
      121 tatcagaaaa aaaattggaa gaaacgcttt ctatcggtcg tactccaata cgtgaagcat
      181 tgattcaact ccgtcatcaa ggacttgttg atacaattcc tcaaagtgga aattatatct
      241 cattaatcga cttagattta gcaaaaaatg ctcgttttgt cagagaacat ttagaacgac
      301 aaattatgct agagtgttgt gccaagatga ataatcaaaa gaaacaagtt ttagaaacta
      361 taattgcaga acaagaaaaa gctgttagtc aaaagaatgc tcgtagtttt tttcataatg
      421 ataatttatt tcataaagct tgttttgaaa ttgcagaacg ggaagaagta tggcaatggt
      481 tggaagataa taatacgcac ttggaacgat ttcgttggtt acgagtaaaa acggaaggat
      541 taaaatggga aaaaattatg gaacaacatc atcaactatt taaagccttg accatgcaag
      601 accctgaaga agctaacttc ttaacatcct tgcatcttca tttaatgctt gatgaacaaa
      661 atgttgtatt gactactcac ccaa
//
LOCUS       DAHHPA010000260          668 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.269, whole genome shotgun sequence.
ACCESSION   DAHHPA010000260 DAHHPA010000000
VERSION     DAHHPA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.269"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>668
                     /locus_tag="NBH55_002967"
     CDS             <1..>668
                     /locus_tag="NBH55_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCD1510869.1"
                     /translation="GTLELKVPRTRDGHFSPTVFERYQRNEKALIASMLEMYVSGVST
                     RKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVRE
                     ENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVISD
                     AHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAR
                     EAKN"
BASE COUNT          237 a          126 c          123 g          182 t
ORIGIN      
        1 taggcacgct agaattaaaa gtacccagaa cacgtgatgg ccatttttca cccacagtgt
       61 ttgaacgtta tcaacgaaac gaaaaagccc tcattgcttc aatgttggaa atgtatgtat
      121 caggcgtttc aactcgtaaa gtatcaaaaa ttgtggaaga actttgtggt aaatccgtct
      181 ctaagtcctt cgtttctagc ttaacagaac agctagaacc aatggttaac gagtggcaga
      241 atcgtttatt atcagaaaaa aattatcctt acttaatgac cgatgtactc tatataaaag
      301 tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat agcgattgga ataaccaaag
      361 atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg cgaaagcgaa gagacctgga
      421 taacattttt tgaataccta aaagaacgcg gtttacaagg tacagaactc gttatttctg
      481 atgcgcacaa aggattagtc tctgccatta gaaaatcctt caccaacgca agttggcaaa
      541 gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat
      601 ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gctcgtgagg
      661 ctaaaaat
//
LOCUS       DAHHPA010000261          663 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.270, whole genome shotgun sequence.
ACCESSION   DAHHPA010000261 DAHHPA010000000
VERSION     DAHHPA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.270"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            409..>663
                     /locus_tag="NBH55_002968"
     CDS             409..>663
                     /locus_tag="NBH55_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510870.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNY"
BASE COUNT          259 a           80 c          120 g          204 t
ORIGIN      
        1 acctagtata atcactttaa taaattgttg gtagaaggtg atcaaaatgg gagtaaacga
       61 aatgatgaaa gtcagattga gtttttaaat gataccttcc tcagaacttc aaaacgaatt
      121 caaagattcg ttgacaaatc ttgagcaaga tcatttaaaa aaatattttc tcttcatttg
      181 acgaaaaatg tttagaaatc ttatataagt gaagataaag taattggtgt aattaattgt
      241 agagaatagc ctttgagcaa ttgaagcgat acaattattt agaaaatgaa cttcctccag
      301 catactacat gattccttac gggacgatgt taaagaagaa agcaagaaat tttagaagta
      361 ttagtagata aactaatggg cttttctcaa tttaagggag tagcccaaat gtttatcaaa
      421 tatggtgcag atacagtagc aaccatgttt acagaagaat atcaagtaga ctattatgaa
      481 gtaatagata aatgtgtaca ttttgtttat gaaaaagtgt ggaattttat gaattttgcg
      541 ataagtgaag aaatcttacg agtacaagaa aacagaacag gaatatttac cagagacaga
      601 aagcttaatt tgattgcgca ttatatacgg aatcgctggt catcaatacc aaattttaat
      661 tat
//
LOCUS       DAHHPA010000262          662 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.271, whole genome shotgun sequence.
ACCESSION   DAHHPA010000262 DAHHPA010000000
VERSION     DAHHPA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.271"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>662
                     /locus_tag="NBH55_002969"
     CDS             <1..>662
                     /locus_tag="NBH55_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCD1510871.1"
                     /translation="KKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDNE
                     LTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSL
                     VTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDV
                     AFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDI
                     NY"
BASE COUNT          241 a           76 c          123 g          222 t
ORIGIN      
        1 caaaaaactt cagatgggtg aaattgttag aggtcctagt caaatatcta ttgatgttac
       61 atataaatgt aattttagat gtaaacattg ttacaatgcg agtggtggag acaattgttt
      121 tatggataat gagttgactg atgatgaact gttagaactt acaaaagata tatgtaattt
      181 aaagccatat aatatttgtt tctgtggagg agaaacttta ctgagaaaaa aaatagtttt
      241 atcttgtgcc actttattaa aagaggcagg tattcctaat attgctattg ttactaatgg
      301 atcattagta actcaggatg ttgcaaaaga attattcaaa gcaggtatta atcgatttca
      361 agtaagttta gatggaaaaa atgctagtag ccatgaagca cttagattat atccaaacag
      421 ttttgaaaag gcaattaatg ctatcaaatt atttagagaa ttgtccgaag aagtggatgt
      481 tgctttttgt cctactagtt ttaattacaa tcaattacga gaagtatatc aactagtaaa
      541 tactcttaaa gtgaagtctt taagaattca accatttatg cttagtggta ggggaatgca
      601 aaatagggaa atagtgccaa ctgtttctca atatagaaaa ctagtttctg atattaacta
      661 ta
//
LOCUS       DAHHPA010000263          642 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.272, whole genome shotgun sequence.
ACCESSION   DAHHPA010000263 DAHHPA010000000
VERSION     DAHHPA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.272"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..351
                     /locus_tag="NBH55_002970"
     CDS             <1..351
                     /locus_tag="NBH55_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510872.1"
                     /translation="YLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLAWATRQSYETLL
                     SLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRLLFTDKATADAA
                     ALLVNGTVEEVVER"
     gene            348..>642
                     /locus_tag="NBH55_002971"
     CDS             348..>642
                     /locus_tag="NBH55_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510873.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
BASE COUNT          225 a          101 c          151 g          165 t
ORIGIN      
        1 tatttaatca aatctaaaga tggtgattca tattatttct gtgattggtt tgtacgagat
       61 ggcatagtga cgcaagagca aggagaagaa ttactcgctt gggcaacaag acaatcatat
      121 gaaacactat tgagcctata caacggctac gaggttgaga aagagccgtt atgggcaata
      181 aagaatgccg atggaaacta tcttactaaa tgtgctttat ggggaaaaga tggagtaaat
      241 tatagttttg aatgcaatcc atctcatcga ttgcttttca ctgataaagc aacagcggat
      301 gctgcagcat tgttggtgaa tggaacagtg gaagaggtgg tagaaagatg aaactaaaag
      361 acggatttta cgctagcagt cacggtatcg gcggtttaat gctagatatg ccgacaaaga
      421 accctaaaac acgcaagaaa tcaaaattca aagtcggtga catggtccgc tgtgaagcag
      481 aagagttcgt ttatccgttc agaggatacg ttaaaaagat actgtcaaac tcagcaatca
      541 ttcgtattga aaatacaatg aagtgtgatc gttcgactgc aaaacacaag cattatttag
      601 ctgtagcgag attagttgac atggaagtaa tcaagagcaa at
//
LOCUS       DAHHPA010000264          630 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.273, whole genome shotgun sequence.
ACCESSION   DAHHPA010000264 DAHHPA010000000
VERSION     DAHHPA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.273"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>630
                     /locus_tag="NBH55_002972"
     CDS             <1..>630
                     /locus_tag="NBH55_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCD1510874.1"
                     /translation="VALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFD
                     KKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIW
                     MSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYY
                     SGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLN"
BASE COUNT          199 a           78 c          122 g          231 t
ORIGIN      
        1 gtagctttga tgcagacatt atattttgat gataaatatg ttggcagagt agtcggcgga
       61 acactgtcat ttgtgttgat aggtttagta atttatttaa gaataatgat aaagggtgct
      121 catttttttg ataaaaaaca gtggagatac gctttaaaaa tctctttgcc gctaatccca
      181 cattccttag caaatatagt tttagcacaa ttcgatagaa ttatgattaa ttcttattta
      241 ggtcctgctg cggggggaat atattcctac attagtaaca taggtattat tctaagcgta
      301 atatggatgt ctactaataa cgcctgggtt ccatggttct atggtgagat gagtaaaaat
      361 aatgaaaaaa aagtgaagaa agttagtaac tattatttag tagtctttac tttgattacg
      421 atgattgtaa tggtagtttc gattgattta gcaaaattta tggctccgaa agactattat
      481 tctgggttat ctttagtggt tccgataacc ttaggctatt tctttcagtt tttatatagt
      541 ttaccagtta atgctgaatt ttatgagaaa aaaacatcgt ttattgcgat tggtacagta
      601 gcaagtgcta tagttaatat actattaaac
//
LOCUS       DAHHPA010000265          626 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.274, whole genome shotgun sequence.
ACCESSION   DAHHPA010000265 DAHHPA010000000
VERSION     DAHHPA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.274"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            73..234
                     /locus_tag="NBH55_002973"
     CDS             73..234
                     /locus_tag="NBH55_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCD1510875.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>626
                     /locus_tag="NBH55_002974"
     CDS             253..>626
                     /locus_tag="NBH55_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1510876.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNT"
BASE COUNT          258 a           61 c          108 g          199 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaattaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatac
//
LOCUS       DAHHPA010000266          624 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.275, whole genome shotgun sequence.
ACCESSION   DAHHPA010000266 DAHHPA010000000
VERSION     DAHHPA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.275"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..503
                     /locus_tag="NBH55_002975"
     CDS             <1..503
                     /locus_tag="NBH55_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1510877.1"
                     /translation="LTLFLSKKYGKKELASVGMFFSAIVYLILFLIKTSNMYVFLILS
                     SVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVGGW
                     TLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENCEL
                     LKQKHK"
BASE COUNT          200 a           73 c          120 g          231 t
ORIGIN      
        1 ctttaacttt gtttttaagt aaaaaatatg gaaaaaaaga actggcatca gtaggaatgt
       61 tcttttcagc aatagtatat ttgatcttat ttttgataaa aactagtaat atgtatgtat
      121 tcttaattct tagttcggtt ggctatatgg gtttgggtgt gtttaatact gttatttggg
      181 caaatattac tgatgttatt gatgatcaag aagtaaagtc tgatcaaaga gaagatggga
      241 ccgtatatgc ggtatattct tttgctagaa aaataggtca agctctagct ggaggcgtcg
      301 gaggttggac tctttctatt ataggctatg actctttagc aaatgtacaa acgggagatg
      361 ttttagaaaa gttatataac gcttcaacat taattccatc aatttgtttt ctgattgtag
      421 gattaatttt atttttcctt tatccattat ctaaagataa agtcttagaa aattgcgagc
      481 ttttaaaaca aaaacataaa taggagagaa atatgaataa ttggatattt tgtattgatt
      541 cagatggctg tgttatggat acgatgactt ataaacacaa tctctttttc ggaccaattg
      601 ctgcagagat ttttgatgtt catc
//
LOCUS       DAHHPA010000267          616 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.276, whole genome shotgun sequence.
ACCESSION   DAHHPA010000267 DAHHPA010000000
VERSION     DAHHPA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.276"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..534
                     /locus_tag="NBH55_002976"
     CDS             <1..534
                     /locus_tag="NBH55_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCD1510878.1"
                     /translation="KLPSAKKFKRWVTSEVLPAIRKHGGYLTPEKVEEALLNPDTIIQ
                     LATQLKEERTGRLIAEQKIAEYEPKISYLDSILSSTDSVTISQIAADYGMSPQQMNKL
                     LHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPKADGGTKIVMNTKWTQKGRLFIYE
                     LLKKEGYYPQMDLEEIG"
BASE COUNT          256 a          101 c          111 g          148 t
ORIGIN      
        1 aaacttccct ctgccaaaaa atttaaacgt tgggtaacaa gtgaagtgtt gccagcaatt
       61 agaaaacatg gaggttatct aactccagaa aaagtagaag aagctttgct taatccagat
      121 acaatcattc aattagcaac tcaactaaaa gaagaaagaa ctggaagatt aatcgcagaa
      181 caaaagattg cagagtacga acctaaaatc tcctatttag atagcatatt atcttctaca
      241 gattcagtaa caattagtca gattgcagca gattatggga tgtctccaca acagatgaat
      301 aaattacttc ataaactagg tattcagaaa aaagtcggta accaatggtt attgtgcaaa
      361 aaacacatga accaaggata cacaaaatct catacaactg agatcccgaa agccgatggt
      421 ggcactaaaa ttgtaatgaa taccaaatgg acacagaaag ggcgtctatt tatctacgaa
      481 ttactaaaaa aagaaggata ttaccctcaa atggatttag aggaaattgg ttagaaagga
      541 gttttagtat gactgacatt gcagaaatca ctcaacgaga tagagaaaaa atcaaagaat
      601 atgtcgaaag ttcaaa
//
LOCUS       DAHHPA010000268          606 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.277, whole genome shotgun sequence.
ACCESSION   DAHHPA010000268 DAHHPA010000000
VERSION     DAHHPA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.277"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            51..407
                     /locus_tag="NBH55_002977"
     CDS             51..407
                     /locus_tag="NBH55_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCD1510879.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            394..>606
                     /locus_tag="NBH55_002978"
     CDS             394..>606
                     /locus_tag="NBH55_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510880.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDS"
BASE COUNT          235 a           75 c          143 g          153 t
ORIGIN      
        1 ttatggattg attcaaaagg acgacagttt aggtatgtgg aggagagata atggatctca
       61 ttacacaata cagtgacatc atcctcaaga aaatcatgat gaagattcag aaagataaaa
      121 aatcaaaaga acgagctgaa ttagttaagt tagaaatggc tgaaacagga gcaggagtgc
      181 gaagtagcag gcattggaaa gcagcagcaa acattgaatt ttattacaac gaaattcaaa
      241 aagggttcga tcagatgcgt gagctggatc ggcaaacaaa ttggagcaag aaacttcatc
      301 aagatcgttt caaatttgta gagaagtata aagaaatatt agaagagtat ttgaggagga
      361 cagcaaatga taaaaaaact cgttcaattc agcatggatt tatatgatat cgaatcagga
      421 gcaacactat ctgtggaatc ggaccatcta atcataaatt ttggtggaaa gcgccagatt
      481 attttgtggg tagttgatga tgtactgttt ccagaaattg ttcatgattt cgaagaatca
      541 aaagcggttg agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag
      601 gacagc
//
LOCUS       DAHHPA010000269          600 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.278, whole genome shotgun sequence.
ACCESSION   DAHHPA010000269 DAHHPA010000000
VERSION     DAHHPA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.278"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(102..>600)
                     /locus_tag="NBH55_002979"
     CDS             complement(102..>600)
                     /locus_tag="NBH55_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510881.1"
                     /translation="EMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIA
                     DDLEKQWSKEDYAEYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDF
                     EEVVKYIVEEALSQYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLID
                     QNDIE"
BASE COUNT          189 a          117 c           64 g          230 t
ORIGIN      
        1 gcaaaaaatt atcaagttaa aaaaataaat tttgaaccaa attattttta aaaaaatagc
       61 tagttttttt atcaaaaaaa aaagagcttt tcagctctat tttattcaat atcattttga
      121 tcaattaata atcgtacact ccaattaacc gagttttgat cactcaaata tttataatct
      181 ttgtcatcaa aaaccagttg ccaaactctg gtacgaacag tttcttcgtc cacttcctca
      241 tctgaagagt attgcgataa tgcttcttca actatatact ttacaacttc ctcaaaatca
      301 gtaggaactt tatcaataac cacttcatag tcttcttgtt ttacgatatt gggatcaata
      361 tgttcatcca aacatacctc agtgagtttt tctttcacct ctgtatctct ttctgttttt
      421 aggtactctg catagtcttc cttagaccat tgtttttcaa gatcatccgc tatactttta
      481 ataaaggatt ctttatttaa ccaattatct gcggtatcta taccttcaaa ctcaaaagct
      541 gttccttcta tacgtgattt gacgaaagca tcaattttct tgtattgatt attcatttcc
//
LOCUS       DAHHPA010000270          597 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.279, whole genome shotgun sequence.
ACCESSION   DAHHPA010000270 DAHHPA010000000
VERSION     DAHHPA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.279"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>597
                     /locus_tag="NBH55_002980"
     CDS             <1..>597
                     /locus_tag="NBH55_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HCD1510882.1"
                     /translation="AYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLN
                     QIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNI
                     LGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKL
                     GCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL"
BASE COUNT          212 a          108 c          104 g          173 t
ORIGIN      
        1 gcttacacta tcaagattat tcgatacttt ttcatcaaga agctcaccaa aggtgaacta
       61 gtcggaatta tagatagttt tcctagtcca ttatgtaaac cagttagaaa tagacaagca
      121 aaactattga atcaaatagc caaagttggc tataactcaa caaaaaaatc ttatttttat
      181 ggtcttaaaa ttcatatgat cgttactaag actggctttc cgattactta ctcaatcacg
      241 aatccaggtg tccatgacgt gaaagtgtta gagactttat cggaagaagc gaatcttccc
      301 aatatcctag gggacaaagg atatattagc cataaaatcc atgaaaagct tgcccttaag
      361 ggcattacta tatccgttcc gccacgtaaa aacatggata aatcagaaaa actagaccac
      421 agcttacttg gaaaacaaag aaaaacagtt gagactgttt tttcttcctt agaaaaatta
      481 ggttgtcaaa atttcaactc acgttctgtt aaagggctag agagtagatt tgaaagcata
      541 ttactggctt acagtgttct attaagtcga gcacaacgac gttttgaagg aactttg
//
LOCUS       DAHHPA010000271          584 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.280, whole genome shotgun sequence.
ACCESSION   DAHHPA010000271 DAHHPA010000000
VERSION     DAHHPA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.280"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..200)
                     /locus_tag="NBH55_002981"
     CDS             complement(<1..200)
                     /locus_tag="NBH55_002981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCD1510883.1"
                     /translation="MNKQELIDKLSSLVGSIEDFKGISTFHAGKYAGLEHALELIQQL
                     NESQKPVIPQLVAGWIEKSTDN"
     gene            complement(197..>584)
                     /locus_tag="NBH55_002982"
     CDS             complement(197..>584)
                     /locus_tag="NBH55_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510884.1"
                     /translation="KPDILSNHLNGVIAEALPNFIYGNCLVLREDLEEDKQFEEIIDF
                     LDIELMQSTGLKDKNGVEIFEGDIVKTLDADLKVTFSTIKFEEGAFCVDYKDLGTEFD
                     FLYFVDSPLEVIGNIYENSELLEEQR"
BASE COUNT          180 a          128 c           77 g          199 t
ORIGIN      
        1 aaattgtcgg tacttttttc gatccaacct gccacgagtt gtggaataac tggtttctgc
       61 gattcattta gttgttggat tagttctaat gcgtgttcta aacccgcata ttttccagca
      121 tgaaaagtac ttataccttt aaagtcctct atgctaccta caaggcttga tagtttatca
      181 atcaattcct gtttattcat cgctgttcct ccaataactc gctattctcg tatatattcc
      241 cgatgacttc caaaggtgaa tctacaaagt acaaaaaatc aaattccgtg ccgagatctt
      301 tgtaatcaac acaaaatgcg ccttcttcaa actttatcgt tgaaaaagtt actttcaaat
      361 cagcatcgag agttttgaca atatctcctt caaatatctc cacaccattt ttatctttca
      421 gacctgtgga ctgcatgagt tcaatatcaa gaaaatcaat aatttcttca aactgcttat
      481 cttcttctaa atcttccctc aaaactaaac aattgccata aataaaatta ggtaatgcct
      541 cagcaatcac cccattaaga tgattgctta aaatatcagg tttt
//
LOCUS       DAHHPA010000272          575 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.281, whole genome shotgun sequence.
ACCESSION   DAHHPA010000272 DAHHPA010000000
VERSION     DAHHPA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.281"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..394
                     /locus_tag="NBH55_002983"
     CDS             <1..394
                     /locus_tag="NBH55_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCD1510885.1"
                     /translation="KRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSYHVRNIDLMQ
                     STGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCATVDEDLEYQI
                     FNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            391..>575
                     /locus_tag="NBH55_002984"
     CDS             391..>575
                     /locus_tag="NBH55_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510886.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDP"
BASE COUNT          216 a           87 c          122 g          150 t
ORIGIN      
        1 taaacgaaag aacgtaatga gagatgtagc cgtcttgcat tttactaaaa acggcaaaac
       61 aaactttatt gaatattgga taaatcctac cgaattgaaa tcatatcacg tgcgaaacat
      121 cgacctcatg caatccacag gaatgaaaga taagaatggt gtggatatat ttgaagggga
      181 tatagtatta gtcagcgtgc gaaatggctt cgattactta gataataaag tctgtattgt
      241 caaaaattca atagattatt ccggattagt ttgtgccact gttgatgaag acttagagta
      301 tcaaattttt aacacagagc tgtttgaaga atacacgtac gaagtcaccg gaaatatata
      361 cgagaatagc gagttattgg aggaacagcg atgaataaac aggaagcaat caaaaagtta
      421 gaaagtatta aagcgatagg aaatgatgca atagctgctt gctataacga gagtataaat
      481 tcaggtatta cgttaatgaa aaaaatagac gaaccgcaga aaccagttat tccacaactc
      541 gtggccggtt ggcttgagaa atctacggac ccttt
//
LOCUS       DAHHPA010000273          560 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.282, whole genome shotgun sequence.
ACCESSION   DAHHPA010000273 DAHHPA010000000
VERSION     DAHHPA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.282"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..456
                     /locus_tag="NBH55_002985"
     CDS             <1..456
                     /locus_tag="NBH55_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510887.1"
                     /translation="SSFSLNEDSDPLKKYTIQCKKNKDEKLFVIRPDLMKSNTHYIKE
                     KPKDCDYIIISEANKTVFLIELKSSAQTSPAKEISEQIDGGYKWLQHLSFLTKYDFTE
                     FSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMNGPKLDLRELYRKCNDL"
     gene            complement(451..>560)
                     /locus_tag="NBH55_002986"
     CDS             complement(451..>560)
                     /locus_tag="NBH55_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510888.1"
                     /translation="NIVKDLNEGVSISKIAKNYNVTRQTIYRIKKDLNL"
BASE COUNT          219 a           74 c           82 g          185 t
ORIGIN      
        1 tctagttttt ctcttaatga agacagtgat cctctaaaaa aatacactat tcaatgtaag
       61 aaaaataaag atgaaaaatt gttcgtaata agaccagatc ttatgaagag taatacccac
      121 tatataaaag aaaaacctaa agattgtgac tacatcatca taagtgaagc aaataaaaca
      181 gtatttctga tagaacttaa aagctcagct cagacttccc ctgcaaaaga aattagtgaa
      241 caaattgatg gtggatataa gtggttacaa catcttagtt ttctaacaaa atatgatttt
      301 actgaattca gttataaaat tattaaggta tgttgtaaat ttgaggcaag acaagatcgt
      361 aaaaatcttt ttaaacctaa tgaatatggt atttataata tgaatggtcc aaaattagac
      421 ttaagagagt tgtatagaaa gtgtaacgat ctataaattc aaatcttttt taattctata
      481 tattgtttgt ctagtaacgt tataattctt tgcaatcttt gaaatagaga cgccctcatt
      541 caagtcctta acgatgtttt
//
LOCUS       DAHHPA010000274          552 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.283, whole genome shotgun sequence.
ACCESSION   DAHHPA010000274 DAHHPA010000000
VERSION     DAHHPA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.283"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..548)
                     /locus_tag="NBH55_002987"
     CDS             complement(<1..548)
                     /locus_tag="NBH55_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510889.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHIL"
BASE COUNT          221 a           91 c           88 g          152 t
ORIGIN      
        1 tttaaaatat gtgtcagcat atcactagga gccataccac ttaattcttt tcccagtggg
       61 actttcaatt cttgtgctct agattgttga ataaaaggaa ttaagaatcc cgcacttaaa
      121 aaaaaggtga ggacggtagc ttttaaaaga cttaagagtc tagcgatttt ttggtcccaa
      181 aaataaaaag aaagaaacaa cgttcctcca ataaatacag aaaccatagc gacagaaagt
      241 aaatgagtat acactactag cgtcatgcca atagacaacc agtaccattt ttggtaatct
      301 ctaatataaa tttcgtaaca ccccattagt atcaaaggca aaaatgttaa agcgacagct
      361 tctcccaatg aagcacgatg gaatatgtca atggcacgat aggcggaaaa tgtataaata
      421 atcgaaaata acagcgaaat ataacgattg ttttttattt gtttcataga aaaataagaa
      481 acgaccattg ttaaaaaagt gataaaaaaa tagtagatat tataacttaa aacaagattt
      541 cccatcattt ta
//
LOCUS       DAHHPA010000275          549 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.284, whole genome shotgun sequence.
ACCESSION   DAHHPA010000275 DAHHPA010000000
VERSION     DAHHPA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.284"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..254
                     /locus_tag="NBH55_002988"
     CDS             <1..254
                     /locus_tag="NBH55_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1510890.1"
                     /translation="HLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPES
                     ANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            255..>549
                     /locus_tag="NBH55_002989"
     CDS             255..>549
                     /locus_tag="NBH55_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCD1510891.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQL"
BASE COUNT          209 a           63 c          105 g          172 t
ORIGIN      
        1 ttcatttaga ttattttgga agagttccta caagaaaatt tcaattgttt actttagatg
       61 aatgtatcaa tgttgactta ttaaataata aaataagttt tgaagaaaac attgttaaag
      121 aaatgcctga atctgctaat gatatgtatg agaaggagtt gaaatatttc tttgaaaacg
      181 tagtaacagg caaagaaaac tggaatgatt tatatcatgc aaacgaagta ttgaaaatag
      241 ctgaggagaa ataaatggaa attttattta ctatttgtgg tagagctggg tctaaggggt
      301 ttaaaaataa aaatttgaaa tatttggcag gaaagcccct agtccatttc gctttagcaa
      361 tgatagatat ctatcaacaa aaacatcctg aacataattg cacgacttgt ttgaatacgg
      421 acagtaatga gttagtagaa ttgacgacta agttaaatcc gtctgtgaaa attgtagaac
      481 gtaaacctga attagctaca gatactgtag ggaaaattga agtgatacgt gattcattct
      541 tacaactag
//
LOCUS       DAHHPA010000276          546 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.285, whole genome shotgun sequence.
ACCESSION   DAHHPA010000276 DAHHPA010000000
VERSION     DAHHPA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.285"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            165..359
                     /locus_tag="NBH55_002990"
     CDS             165..359
                     /locus_tag="NBH55_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510892.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          232 a           68 c           88 g          158 t
ORIGIN      
        1 gaacaatagc cttttattat atatattatt caaattagtt atatcgagtg aaaaaaactt
       61 tcaaattgat tctttttatt tatcataaag atatagatac ataatcattt tcacttacat
      121 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
      181 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
      241 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
      301 acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc gaaaagtaaa
      361 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
      421 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
      481 atgattattt caatatgtat cctggaattg gagatctcgt agagctatat caagatgaat
      541 caaata
//
LOCUS       DAHHPA010000277          543 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.286, whole genome shotgun sequence.
ACCESSION   DAHHPA010000277 DAHHPA010000000
VERSION     DAHHPA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.286"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..132
                     /locus_tag="NBH55_002991"
     CDS             <1..132
                     /locus_tag="NBH55_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1510893.1"
                     /translation="RRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            189..>543
                     /locus_tag="NBH55_002992"
     CDS             189..>543
                     /locus_tag="NBH55_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1510894.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDE"
BASE COUNT          201 a           63 c           94 g          185 t
ORIGIN      
        1 cgcagaagag aaaagattat tcgcatcttc cccaatcaaa catcagccaa tcgcttaatt
       61 ggagccgttc ttatggacct acatgatgaa tggatttatt cttcaagaaa atacatcaat
      121 tttgataagt agaaatggta aaaacattgt atagcatttt acacaggagt ctggacttga
      181 ctgagtttat ggaagaagtt ttaattgatg ataatatggt ttttgatatt gataatttaa
      241 aaggatttct taatgatacc agttcatttg ggtttatagc taaagaaaat aataaaatta
      301 taggatttgc atattgctat acacttttaa gacctgatgg aaaaacaatg ttttatttac
      361 actcaatagg aatgttacct aactatcaag acaaaggtta tggttcaaaa ttattatctt
      421 ttattaagga atattctaaa gagattggtt gttctgaaat gtttttaata actgataaag
      481 gtaatcctag agcttgccat gtatatgaaa aattaggtgg taaaaatgat tataaagatg
      541 aaa
//
LOCUS       DAHHPA010000278          527 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.287, whole genome shotgun sequence.
ACCESSION   DAHHPA010000278 DAHHPA010000000
VERSION     DAHHPA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.287"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(140..505)
                     /locus_tag="NBH55_002993"
     CDS             complement(140..505)
                     /locus_tag="NBH55_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510895.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          212 a           73 c          104 g          138 t
ORIGIN      
        1 tacacaactg tttttagtgt ttggagagaa gaaaagaagt aaaaaataga aaaactagtg
       61 atagttttgg tgatataaga aaaacatata tgtataaaaa gaagaaagta gcttctcttt
      121 gtacatatat ggtttgtttt tatggatgcc gagctttttt ataagataag gctaccgccc
      181 ataacgaccc aaggatttgt ggaactaagt aaatacctgc aagaagcaag atcagctggg
      241 tcggttttaa aggatgatag aagtaacgcc attcagtaaa attggcgcaa aataaatagg
      301 aagtgacagt agatataata ttggcaatga ataaagatat aatacgcctg ttcctttgaa
      361 aataatatcc agcaaaaaga gataaaaata tcaatgtgat gaacccgctt acggaatgat
      421 aacgaaaatc aagcagtaag gcgaaatgca cataaggaac aaaagataaa agcaacggaa
      481 ggtttttcca gcaagttttt atcatctttc atcaccacct aacaagt
//
LOCUS       DAHHPA010000279          525 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.288, whole genome shotgun sequence.
ACCESSION   DAHHPA010000279 DAHHPA010000000
VERSION     DAHHPA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.288"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            1..267
                     /locus_tag="NBH55_002994"
                     /pseudo
     CDS             1..267
                     /locus_tag="NBH55_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            325..>525
                     /locus_tag="NBH55_002995"
     CDS             325..>525
                     /locus_tag="NBH55_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510896.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKI"
BASE COUNT          172 a           68 c           83 g          202 t
ORIGIN      
        1 tatacaacat cttcttttag gagtgtagaa atatttcttc gaggggcaat gggggaatta
       61 ctcgcaatgt caatacttcc tcttatttta ctcgggttta taaagcttta cgactcaaaa
      121 aaagaatctt gggttatgtt ggcgattagc atgactttat taatttacac tcatgttctt
      181 tctgtagcta tgactatggt aatgattata attgctttaa ttattcaatt ttatcggaaa
      241 aagaaaatag aaatcttctt aattattaaa ttaataaaag ctgctatagt tactttactt
      301 ctttcactag cgtttatagg tcctatgatt gagcagacat tgtatcaaga tctgaataga
      361 ccttatgtgg atatattaca aaaaagagca atttatctgg gtaaagagtt tttgattgga
      421 agtatagatt cagaattact atcatttggt attggcttaa ctttgcttat ttgtttaatt
      481 atactaacat ttaactttaa aaaattagga atcttatcta aaatc
//
LOCUS       DAHHPA010000280          483 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.289, whole genome shotgun sequence.
ACCESSION   DAHHPA010000280 DAHHPA010000000
VERSION     DAHHPA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.289"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..419
                     /locus_tag="NBH55_002996"
     CDS             <1..419
                     /locus_tag="NBH55_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1510897.1"
                     /translation="EEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQ
                     DILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLEC
                     LNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          164 a           97 c           82 g          140 t
ORIGIN      
        1 aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt
       61 tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct
      121 atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg
      181 attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt
      241 ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat
      301 tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact
      361 tttacaattt caaactacga attatgattc gtcatggcaa aacattttta accaaataaa
      421 cagaacagaa agaggttttc ttctttctgt tctgtccaaa ttattctaag atttctcttt
      481 acc
//
LOCUS       DAHHPA010000281          483 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.290, whole genome shotgun sequence.
ACCESSION   DAHHPA010000281 DAHHPA010000000
VERSION     DAHHPA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.290"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
BASE COUNT          188 a           58 c           67 g          170 t
ORIGIN      
        1 gtactttatt atcaagccat tattccttct tctactagtt ctttttgtca cataattttg
       61 caatcaataa atgttgcgca ataagtataa gaattccatt ttctaaattt gaaaggtgat
      121 ccataatttc atgttcaagt agtttattaa ttgtccactt gtcgcttcat ctaaaaattt
      181 tataggtttt aggaatgcta ttcgcttatt ttaggaagat tttaactgtt tgctatagat
      241 atatatcata ctagtatcgt aataaaagta ctctaaaaga agaattttca aatacgcaaa
      301 attaagtacg aaagagttga aacaaatttg gagaaaatga gcatagaatg tctgatgagt
      361 taaatagacc taaaaattat ctaaaagtgg agcaaaatta ggaattacaa acaaaacact
      421 aaattagttt atataaattt ccgtaatatt taatatatat gaaaaggaaa acgtagaaaa
      481 tta
//
LOCUS       DAHHPA010000282          480 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.291, whole genome shotgun sequence.
ACCESSION   DAHHPA010000282 DAHHPA010000000
VERSION     DAHHPA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.291"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            217..>480
                     /locus_tag="NBH55_002997"
     CDS             217..>480
                     /locus_tag="NBH55_002997"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCD1510898.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          155 a           96 c           75 g          154 t
ORIGIN      
        1 aaaaaccttt tcataatcct caccctttct tacactgata aatcatgcgt ctaatattca
       61 aattatactc gatatgcttt agctaatcag ccaactctgt ttgtctacta tcattatatc
      121 aatagacaat atctgctccc gtctttatga tcgtgccaaa gcaatcaaaa gaaaattgcg
      181 aatcacttag atcgaaagtt tactcaaaca aaagcattgt cagcggttgt cacggattta
      241 acttatgttc gggtcgggac aaattgggct tatatttgtt tgatgattga tttattcaat
      301 cgtgaaatta ttggccattc ttgcggaaat aaaaaagatg cgcagcttgt aaaaagggcc
      361 attcaaagca ttccttactc acttcaagaa atcgaactct tccatacgga tcgtggaaaa
      421 gaatttgata accaaaccat tcagaatctc atgaatggtt tggttatcaa attcctttcc
//
LOCUS       DAHHPA010000283          475 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.292, whole genome shotgun sequence.
ACCESSION   DAHHPA010000283 DAHHPA010000000
VERSION     DAHHPA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.292"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..336)
                     /locus_tag="NBH55_002998"
     CDS             complement(<1..336)
                     /locus_tag="NBH55_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510899.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEF"
BASE COUNT          157 a           85 c           67 g          166 t
ORIGIN      
        1 aaattcaaga tcagccagtt ctgtactcgt gaatatacca ttaaaataat aacttaaaaa
       61 ctttaaaaaa taagattgac cgtacagaaa ctgtgtgagc tcagaaagag caataccatg
      121 tttgaatacg agtttatact tatgcttctc tcttacttgt tcttttatac aatattctag
      181 acactcggct agactttcta gatcgtgaag gatcgttggt gtagtcaccc ttagagaaat
      241 agctgtttgt tcgatgtcta tttcttggaa ttcaaataat tgttcacaca gatacacttt
      301 tctaaatata tttcgctcga tgtaattttc cagcattcct gatacacgcc tccagttttt
      361 cttatgattt tatttaatca tatctgtaaa tcgtattttt gtttcctttt tatttagaaa
      421 agccctttca aaaaccaata aaaaaagaaa ctgggacaaa agtcaaacaa gctca
//
LOCUS       DAHHPA010000284          465 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.293, whole genome shotgun sequence.
ACCESSION   DAHHPA010000284 DAHHPA010000000
VERSION     DAHHPA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.293"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>465
                     /gene="bsh"
                     /locus_tag="NBH55_002999"
     CDS             <1..>465
                     /gene="bsh"
                     /locus_tag="NBH55_002999"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bile salt hydrolase"
                     /protein_id="HCD1510900.1"
                     /translation="LADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYR
                     VLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESES
                     ISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQIT"
BASE COUNT          155 a           65 c           85 g          160 t
ORIGIN      
        1 tattagctga taaagaaaaa tcaattgtca ttgaaagtat gaaagatgga cttcatatat
       61 atgataaccc tgtgggcgtt cttaccaata atccttcatt tgactatcaa ttatttaatt
      121 taaacaatta tcgtgtctta tcgagtgaaa ctcctaaaaa taatttttca aatcaaataa
      181 gtttgaatgc ctatagccgc ggtatgggag ggataggctt gcctggagat ttatcctcag
      241 tatctcgttt tgttaaagcg acttttacga agctgaattc tgtatctgga gattcagagt
      301 cagaaagtat tagtcaattt ttccatatct taggttcagt agaacaacaa aaaggtttgt
      361 gtgatgttgg tgatggaaaa tatgaatata caatttattc ttcttgttgc aatgttgaca
      421 aaggaatcta ttattatcga acatatgaag acagtcaaat tactg
//
LOCUS       DAHHPA010000285          459 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.294, whole genome shotgun sequence.
ACCESSION   DAHHPA010000285 DAHHPA010000000
VERSION     DAHHPA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.294"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..430)
                     /locus_tag="NBH55_003000"
     CDS             complement(<1..430)
                     /locus_tag="NBH55_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD1510901.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           91 c           58 g          178 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttcta
//
LOCUS       DAHHPA010000286          459 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.295, whole genome shotgun sequence.
ACCESSION   DAHHPA010000286 DAHHPA010000000
VERSION     DAHHPA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.295"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
BASE COUNT          174 a           62 c           84 g          139 t
ORIGIN      
        1 ttatttatgt aagattgctt cgataattct caaaacgaaa taagtaggga agttggagat
       61 tttgattatc ttcgtaactg acgaagagta aatgaatcca tttggtatgc actcttaagt
      121 tcaaaatatt ggtatcgatg tttaaaagaa atagaaatgt atcgaagaac agatgtttaa
      181 aaaagtgtga ctttgtataa ataaattaga agaagcaaac gaggatgtta aaagaatacc
      241 atatagttac caaaccaaga aagttctcgt caagttcgct ggtacgaatc aaatcaagtg
      301 aaaaaaggga ctgtgaaaaa aattgtgtca tagttagtca ctttttccga acaaatagtg
      361 ttcaatcagc tgtccttcgg tattagctca aaaaacggca aaatatgagg aactattttt
      421 ccatttgttt ctctaatact caggacaaaa cactgattt
//
LOCUS       DAHHPA010000287          456 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.296, whole genome shotgun sequence.
ACCESSION   DAHHPA010000287 DAHHPA010000000
VERSION     DAHHPA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.296"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>456
                     /locus_tag="NBH55_003001"
     CDS             <1..>456
                     /locus_tag="NBH55_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1510902.1"
                     /translation="KEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKK
                     TNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLS
                     VDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVM"
BASE COUNT          163 a           56 c           84 g          153 t
ORIGIN      
        1 aaagaaattc aatatattaa gtctaaagtg acgcataata aaaaaatttc ttatcaatac
       61 catgtaggac agtatttacc cgactggcat ccttgggaag attatcgcaa tttttttgtt
      121 tcaaacaaaa aaactaatgg gtgtagagaa atattcgcta ttgaattacc ttggattata
      181 gatacatttg ggaaagttaa atcgtttact gtaatgaccc agaaattatc caatcttgat
      241 attgattatt cggacagttt cactcttatt attgagcatg aaactggtat agttggaact
      301 ttaagtgtag atgttgtttc aagagtagct aaaagaaatt taactataat tggtgaaaag
      361 attttaatag aatgggaagg taacccggat tctctatttt catggaatga agaaacttct
      421 agtatgacag atattcaatt gtacgatgag gttatg
//
LOCUS       DAHHPA010000288          449 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.297, whole genome shotgun sequence.
ACCESSION   DAHHPA010000288 DAHHPA010000000
VERSION     DAHHPA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.297"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>449
                     /locus_tag="NBH55_003002"
     rRNA            <1..>449
                     /locus_tag="NBH55_003002"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          136 a           99 c          132 g           82 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggca
//
LOCUS       DAHHPA010000289          444 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.298, whole genome shotgun sequence.
ACCESSION   DAHHPA010000289 DAHHPA010000000
VERSION     DAHHPA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.298"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..368)
                     /locus_tag="NBH55_003003"
     CDS             complement(<1..368)
                     /locus_tag="NBH55_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510903.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVA"
BASE COUNT          125 a           75 c           72 g          172 t
ORIGIN      
        1 aaagcaactg taccattttg attattcgtt gtataactct gaacaggatt tttcttactt
       61 ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat tcttgtatgc tttatctaaa
      121 ttaagttggg cattggctaa agcaaggcta tcaatttctt tcaaaaacgg aaattctttc
      181 ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag aatcattttt aacttcttca
      241 tacgcctttt ttcggtcatc aagcattaga ttgtagacct tacggacaca accaaaagat
      301 ttggcaaaga agatttcttg ttcctctgtt ggatagattc tgaatttgta tgcttttagt
      361 tgttccataa agttcaccta ctttctattt atgattgttt ccttgatttt ggatatactt
      421 cttaatcaca tcaattggtg cgcc
//
LOCUS       DAHHPA010000290          441 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.299, whole genome shotgun sequence.
ACCESSION   DAHHPA010000290 DAHHPA010000000
VERSION     DAHHPA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.299"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>441
                     /locus_tag="NBH55_003004"
     CDS             <1..>441
                     /locus_tag="NBH55_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510904.1"
                     /translation="TTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDT
                     EFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVD
                     VSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          179 a           67 c          101 g           94 t
ORIGIN      
        1 aacaacagtc tttgcagtga aagacggcgt aagcttacca gaagataaac ttccagtgtt
       61 gaaagcaaaa gatggttata cagatgcgaa atggccagag gaagcaactc aaccaattac
      121 agcagatgat acagaatttg tatcaagtgc aacaaaactg gatgataaat ctgacgctga
      181 caaatataat cctgaaggtc aaaaagtgac tacagaattg aataaggaac cagacgcatt
      241 tgagggaatt aagaacaaag aagatttacc aaaagatact aagtatactt ggaaagaaaa
      301 agtagatgtt agcgcagctg gaaataaaaa agggactgtt gtagtgacat atccagatgg
      361 atcatctgat gaagttgaag tagatgttac agtgacagac aatcgttctg acgccgataa
      421 atatgagcca acagtagaag g
//
LOCUS       DAHHPA010000291          435 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.300, whole genome shotgun sequence.
ACCESSION   DAHHPA010000291 DAHHPA010000000
VERSION     DAHHPA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.300"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            82..>435
                     /locus_tag="NBH55_003005"
     CDS             82..>435
                     /locus_tag="NBH55_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCD1510905.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRY"
BASE COUNT          151 a           75 c           87 g          122 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatat
//
LOCUS       DAHHPA010000292          424 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.301, whole genome shotgun sequence.
ACCESSION   DAHHPA010000292 DAHHPA010000000
VERSION     DAHHPA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.301"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..377)
                     /locus_tag="NBH55_003006"
     CDS             complement(<1..377)
                     /locus_tag="NBH55_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510906.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYK"
BASE COUNT          141 a           73 c           42 g          168 t
ORIGIN      
        1 tttttgtatt tttttatgtt atctttagct aaaacatcaa atacatgtcc ttgaaattgt
       61 atcgaggcag ctggatttag tttaaattga ggaaacttta ccacagctga ttcaaaacac
      121 ttgtcacatt tttttatttt tttacctttg taaaaatatt cgattgaata ttctatttgg
      181 cacgactcac ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca
      241 aaatctaaac tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa
      301 tttatctttg gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct
      361 ctatcaaaat agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc
      421 aaac
//
LOCUS       DAHHPA010000293          412 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.302, whole genome shotgun sequence.
ACCESSION   DAHHPA010000293 DAHHPA010000000
VERSION     DAHHPA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.302"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            47..>412
                     /locus_tag="NBH55_003007"
     CDS             47..>412
                     /locus_tag="NBH55_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HCD1510907.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIF"
BASE COUNT          166 a           50 c           71 g          125 t
ORIGIN      
        1 ttagacttac aataagcagt gaatttataa tcaacgtata ggagacatgg atgaaatgaa
       61 actaattggt atagatttag acggtactct tttgaattct gaccagagca ttagtcaaaa
      121 aaatgcaaga acaatgaaac atttaccgga agactgtttt ccattcattt gttctggaag
      181 agaagtagaa gacattaata atattctcaa aaaatctggt ttaaggatac cagcagtagg
      241 gttaaatggt tctgttggat ttgatggaga aaaaaagata tttgagtctt ccttcgataa
      301 aaatgatgta aaaaaaattg attctgtcct atcgaaattt ccaaccaaaa tatatacaaa
      361 tctcggaagt tatgaatctc agaattataa aaataaaatg aaaaaaattt tt
//
LOCUS       DAHHPA010000294          408 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.303, whole genome shotgun sequence.
ACCESSION   DAHHPA010000294 DAHHPA010000000
VERSION     DAHHPA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.303"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>408
                     /locus_tag="NBH55_003008"
     CDS             <1..>408
                     /locus_tag="NBH55_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD1510908.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGR"
BASE COUNT          128 a           76 c           88 g          116 t
ORIGIN      
        1 caaatcaaac tgtggtagac gtccgcttgt tttacccgag gaacaatcag aatatattca
       61 aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat
      121 tagttgttct gctcgtacca tttatcgtat gttcaaaaag gggctatttg attcttctga
      181 cttaccgatg aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg gaaaacaaac
      241 tttccgccgc tctattcatg aacgtgaaaa ggattatagc caattctcaa atgagtttgg
      301 tcaccttgaa ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa ttaccttagt
      361 tgaacgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagaca
//
LOCUS       DAHHPA010000295          370 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.304, whole genome shotgun sequence.
ACCESSION   DAHHPA010000295 DAHHPA010000000
VERSION     DAHHPA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.304"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>370
                     /locus_tag="NBH55_003009"
     CDS             <1..>370
                     /locus_tag="NBH55_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HCD1510909.1"
                     /translation="IYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLY
                     RAIDADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSV
                     GLYTKTEHRTVKYLNNLIEQ"
BASE COUNT          124 a           68 c           78 g          100 t
ORIGIN      
        1 cgatttatcg ttgggtgcaa gagtacagca aagtcctcta ttatctttgg aagaagaaaa
       61 atagacaatc cttctattca tggaaaatgg acgaaaccta tatcaaaatt aagggacgtt
      121 ggcattatct ttatcgtgca attgatgcgg acggcttaac cttagatatc tggttacgaa
      181 agaaacggga tacgcaagca gcctatgctt tcttaaaacg actccataaa cagtttggtg
      241 agccgaaagc aattgtgacc gataaagcac cttctcttgg ctccgccttt agaaagttac
      301 agagtgtggg tttatatact aagacagagc accgaactgt gaagtatctt aacaatttaa
      361 tagaacaaga
//
LOCUS       DAHHPA010000296          364 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.305, whole genome shotgun sequence.
ACCESSION   DAHHPA010000296 DAHHPA010000000
VERSION     DAHHPA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.305"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>364
                     /locus_tag="NBH55_003010"
     CDS             <1..>364
                     /locus_tag="NBH55_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCD1510910.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILST"
BASE COUNT          137 a           62 c           76 g           89 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt ctgcccatac ttcatgcgac gacaatggat ttattcttag
      361 caca
//
LOCUS       DAHHPA010000297          359 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.306, whole genome shotgun sequence.
ACCESSION   DAHHPA010000297 DAHHPA010000000
VERSION     DAHHPA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.306"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..354)
                     /locus_tag="NBH55_003011"
     CDS             complement(<1..354)
                     /locus_tag="NBH55_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCD1510911.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKL"
BASE COUNT          121 a           71 c           44 g          123 t
ORIGIN      
        1 caatttttta gtttctccta ctgttgagca ttgtcctaaa atccaaggaa taaattcaaa
       61 aggagataca ttgtctttcc cttcttgtat ttctttataa tcagcatacc cagaaaaatt
      121 tagcccagcc atactcaatc ctttttcatt tgtcgcatcg taataaagag ggtagtcagc
      181 tataccagcg gcaataccaa tcattgcata atgagtatcc aaatcattta cctttcgaaa
      241 attcaactta taatttcttg gagtaacagt aactacttca ttgtaagata tttcataatc
      301 aaaattcctt ccaaaataat gatcacttgt tacataagta atagacgtac acatagttt
//
LOCUS       DAHHPA010000298          355 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.307, whole genome shotgun sequence.
ACCESSION   DAHHPA010000298 DAHHPA010000000
VERSION     DAHHPA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.307"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
BASE COUNT          137 a           50 c           75 g           93 t
ORIGIN      
        1 attaagaatt gtttaatagt aataattagt aatatacgaa ttataagatg taatttatat
       61 tgaatgtatg taaataaata aaatggctga cggatattga atagcaataa ataacaaaaa
      121 gccagacgga tacagtgagt gaagtagctt gctacgaacg aacagacaga atgaaacggc
      181 agtttcatta gagcaagtcg cttgcgacgt agcgttaccg ctgatgtaac tggtaagtta
      241 catagagaga gtttgcttgc aaacgaacgt tacctacttg aatagaaata aaaaaataac
      301 atattagtga attttgaaca ggagccacaa ctcacccaac cgcttgcggt gggtg
//
LOCUS       DAHHPA010000299          350 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.308, whole genome shotgun sequence.
ACCESSION   DAHHPA010000299 DAHHPA010000000
VERSION     DAHHPA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.308"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..191
                     /locus_tag="NBH55_003012"
     CDS             <1..191
                     /locus_tag="NBH55_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1510912.1"
                     /translation="NLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNR
                     SHKGFQQVTDTLVSMFTE"
BASE COUNT          129 a           57 c           51 g          113 t
ORIGIN      
        1 ccaatctaat cgagtctttc aataagcaaa ttaaaagata cagccgtaga aaagagcagt
       61 ttcaaaatga agaatcacta gaacgctttc tagtcagcat ttttgataca tacaatcaaa
      121 aatttctaaa cagaagccat aaaggttttc aacaggtaac cgatacatta gtttcaatgt
      181 ttactgagta actaattatt ttgcaggagg acaatttatt tacacaaaat tattgacgct
      241 cccatcctaa aacataagct atttgtttac ttctgtagaa tatatacttt gttgttagat
      301 aagttgcaca tgtttataga atctaaataa tacccgtggt gctagttaat
//
LOCUS       DAHHPA010000300          345 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.309, whole genome shotgun sequence.
ACCESSION   DAHHPA010000300 DAHHPA010000000
VERSION     DAHHPA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.309"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            77..>345
                     /locus_tag="NBH55_003013"
     CDS             77..>345
                     /locus_tag="NBH55_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1510913.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAAL"
BASE COUNT          146 a           51 c           66 g           82 t
ORIGIN      
        1 atggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag aagaaaatat
       61 accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca
      121 acaaatcgtc aatctttact tagctggaaa gccacgtgta gaaatcattc gagaatatga
      181 actaacggct tcagcatttg acaaatgggt aaagcaatct aaaacgagtg gttcattcaa
      241 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
      301 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgat
//
LOCUS       DAHHPA010000301          326 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.310, whole genome shotgun sequence.
ACCESSION   DAHHPA010000301 DAHHPA010000000
VERSION     DAHHPA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.310"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
BASE COUNT          110 a           56 c           53 g          107 t
ORIGIN      
        1 gtggtagtta attagtgact tatttttgat tttatagcac tgatactata aaatatagat
       61 atcatcatat tacacaatct taatactaac ttaaaaaata tctcctttca caagtatggt
      121 gataaaattc gttccgggct acctttttag gtagcctact ttaatcttta tacctttctg
      181 gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgctacgt gcgtccggca
      241 cgtcagtcat gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata
      301 aaaaacacac tggaggattt ttcatg
//
LOCUS       DAHHPA010000302          322 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.311, whole genome shotgun sequence.
ACCESSION   DAHHPA010000302 DAHHPA010000000
VERSION     DAHHPA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.311"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(44..>322)
                     /locus_tag="NBH55_003014"
     CDS             complement(44..>322)
                     /locus_tag="NBH55_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1510914.1"
                     /translation="GITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           87 a           81 c           60 g           94 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt cc
//
LOCUS       DAHHPA010000303          305 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.312, whole genome shotgun sequence.
ACCESSION   DAHHPA010000303 DAHHPA010000000
VERSION     DAHHPA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.312"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="NBH55_003015"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="NBH55_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCD1510915.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DAHHPA010000304          291 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.313, whole genome shotgun sequence.
ACCESSION   DAHHPA010000304 DAHHPA010000000
VERSION     DAHHPA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.313"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>291
                     /locus_tag="NBH55_003016"
     CDS             <1..>291
                     /locus_tag="NBH55_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD1510916.1"
                     /translation="TRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEY
                     IFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYV"
BASE COUNT           97 a           50 c           61 g           83 t
ORIGIN      
        1 aacacgagct ggtgcagcta atcctacgct gacagatgga acaaatcacg cccacattga
       61 tcagtttggg gaaatcgaaa acgcaaactt gcatgtggct ggatggcaca ttgctaacta
      121 taaatacgag tatattttca ttatggacta caatactggg aaagaattag ctcgagtaag
      181 agctgatgga atttatagac cagatgtaaa ccaagcttat aatacttctg gaaatgttgg
      241 ttatcatgta tctttcaata tgcgtaattt tcctagtaag aaagtctatg t
//
LOCUS       DAHHPA010000305          286 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.denovo.314, whole genome shotgun sequence.
ACCESSION   DAHHPA010000305 DAHHPA010000000
VERSION     DAHHPA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.denovo.314"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            <1..>286
                     /locus_tag="NBH55_003017"
     CDS             <1..>286
                     /locus_tag="NBH55_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCD1510917.1"
                     /translation="LADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQFVTG
                     IQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLT"
BASE COUNT          104 a           34 c           59 g           89 t
ORIGIN      
        1 gattagcaga tcacggtttg aatacttatt tttatgaaat agcagatgaa aaaatactcg
       61 acaatctgtt aataaaagct ttagcaggaa aaacggattt ccatttatca gaaattgcac
      121 aatttgttac aggtattcaa ttacatttga tgtctgagta tacaagatat ggttggacga
      181 tgcaaatgca tttaaatgct ttaagaaata attcaagtat attaaaggaa caaattggag
      241 tagatgtcgg tggtgattcg atgggggatc aagctagttt gacaca
//
LOCUS       DAHHPA010000306        40432 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.guided.012, whole genome shotgun sequence.
ACCESSION   DAHHPA010000306 DAHHPA010000000
VERSION     DAHHPA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.guided.012"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            16..258
                     /locus_tag="NBH55_003018"
     CDS             16..258
                     /locus_tag="NBH55_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1510918.1"
                     /translation="MGLSNEYIEGIFVSAEMQSQGIGKILLNYVKGKRNKLILNVYQK
                     NTRAISFYQREGFEIQYSGLDEATGEKDYVMAWQQK"
     gene            437..667
                     /locus_tag="NBH55_003019"
     CDS             437..667
                     /locus_tag="NBH55_003019"
                     /inference="COORDINATES: protein motif:HMM:NF025559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HCD1510919.1"
                     /translation="MQDDDSGFTYEISRCLYFDTCKEHGYAEFCKVFCNHDWYAYGVL
                     KHHSRFIRKSTIAEDGTVCHDTIEKVTEKHFL"
     gene            701..883
                     /locus_tag="NBH55_003020"
     CDS             701..883
                     /locus_tag="NBH55_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylan 1,4-beta-xylosidase"
                     /protein_id="HCD1510920.1"
                     /translation="MAKEERFEKIYSQGALTTTEIWVDKETGVNYLYHACGYSGGLTP
                     LLDRDGKPVISQAINR"
     gene            996..2507
                     /locus_tag="NBH55_003021"
     CDS             996..2507
                     /locus_tag="NBH55_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510921.1"
                     /translation="MRRSGWTKWLIVIPAMLGIVLVTYVRYTTDIWGFGAFICQLIAI
                     LELAYGLRIAMLAQNRKKSYRLTPEERHEYARYLYEKQYHRYPAVANQMLLVMARMGV
                     LLNNYERAAQELADIRIDKFNPAQLKLYYYLKVVTAMAAGDATGIQESQMCYAGIPDT
                     KCTYPSKAELAMWIEQRAAAQMAEALKKAVPDKKEHPIRISIITLVLAYSTFFYGLWY
                     GIKRDMGYEVRYRFAEISITVITISLAVLLIWGIVSLYRHNCQALSIQFGKNKVALII
                     VYGAAAIFALLIIGMSGLSVVFGMDGTETVTGCDSKYTYITVQEDYGGERRYRTNNPF
                     VMQSMDGLWWSSDTIDEEDNQEEDTTELPDLDTENSTDSEDIQSGNTIEDYETEGQHD
                     GLTIQNAMLAVYNYLQEQNTLQNMAFSYTANAKGEVYAVVSETQETKNGNTVNVRYCL
                     YDNGSKTDADGNSCEELVLEKVYPDGNYETELVDFYLVNADTMQVTDEQKNTW"
     gene            2830..3054
                     /locus_tag="NBH55_003022"
     CDS             2830..3054
                     /locus_tag="NBH55_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HCD1510922.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            3047..3613
                     /locus_tag="NBH55_003023"
     CDS             3047..3613
                     /locus_tag="NBH55_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HCD1510923.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            3601..3831
                     /locus_tag="NBH55_003024"
     CDS             3601..3831
                     /locus_tag="NBH55_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510924.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            4032..4397
                     /locus_tag="NBH55_003025"
     CDS             4032..4397
                     /locus_tag="NBH55_003025"
                     /inference="COORDINATES: protein motif:HMM:NF024360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HCD1510925.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKL
                     VERRERSHRLIVKGAEFEKVFPLSRDLEQEDVQDMMNQLQNSSYNKSIVRQAHIAALH
                     KDQQQIAEAVKKTEKGDDS"
     gene            4397..6124
                     /locus_tag="NBH55_003026"
     CDS             4397..6124
                     /locus_tag="NBH55_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCD1510926.1"
                     /translation="MALYHFSVRNVSRGKGQMVVASAAYISGQRIFDSYYNKIHDYTS
                     KSGVIFTEILTPEYVPERLTDRETLWNEVEHVERNNKAQLAYSFDIALQNELTLEENI
                     RLAKEFCQEQFVARGMIVDLAVHEGKAENEEEPDNPHFHVLVPIRPFTEEGIWGNKQR
                     REYILDEDGNRVKDAKGKDMFNAVSTTGWNDPELLKEWRRAWTEKVNGKFRECHMASR
                     IDHRSYKEQGIDLIPTIHEGYEVRAMEKKGIKTVIGELNRAIRQFNQMFISLKESIQW
                     MKTAYEEMKAELDRRQNPTLLESLQDYYDKKTQGRPPLPNFYAEMKQRGKNLSNLQEF
                     AKSISYLQTHQIETMDDLQERIEELNGVVSVSKKEISEKRKQLKELENLEKMAEVIKT
                     NQPLIDEYNHFYFQKRRERYYQQHKKEINYYRKCERELKQHLDKNGKVPTARWKREKE
                     ELQAGIEELKADNQPYQEELTFVKKVQSCADIARRDREMAEADTSGRSEEKREKQVKF
                     PTFYTVQAKEILEENGKAEQQPLNQSEQNPEKKTSLLRKLDEKKKECAERDAKQQAVK
                     KKRNYDMSL"
     gene            6154..8070
                     /locus_tag="NBH55_003027"
     CDS             6154..8070
                     /locus_tag="NBH55_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1510927.1"
                     /translation="MSKMQYTEEQIIRANQTDLVSFLSAQGEQLGKSGKEYRWKKHDS
                     VTVSGNRWYRHSQGRGGYPVDFVMEFYNATFPEAVKMLTGEEGEGRNSTCPAPSPDFR
                     LPEKEENNDRIIRYLTENRGIEKNMVEEWIGSGDIYEEKKHHNVVFVGRDADGIPRYA
                     HCRGTGETKYRGDVAESDKSYGFCHRGTDNQLFVFEAAIDLLSFIQLFPKDWKKRSYL
                     SLGGISSAALMAFLSERPQITSVFLCLDNDHAGNEASEKLAIEIPDGYSVIRLKPSRK
                     DWNEILCDKNADRKKSIIETVTMKVPEKEELVPMLCYEDIEQTSVEWLWFPYLPFGKL
                     TIIQGNPGEGKTYFAMMLTAACTNRKTFPNMEEIEPFNVIYQTAEDGMGDTIKPRLVE
                     AGADLSRVMVIDDTEEALTLSDDRIEKAIRQNQVRLLIIDPVQAFIGADVDMNRANEV
                     RPVFRKLGMIAEKTGCAIVLIGHLNKSSGTQSTYRGLGSIDIMAAVRSLLFIGKVKKD
                     PTTRVLIHEKSSLAPPGETMAFKLGDEEGFRWVGAYEISADDLLDGKEGKPTETKLQR
                     GTKLIYELLADGNAVTIRELDEKAKAQGISQRTMREARSRMKEELDYRMNEKQENTIR
                     LKKQGRMGDGRILE"
     gene            8755..8937
                     /locus_tag="NBH55_003028"
     CDS             8755..8937
                     /locus_tag="NBH55_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510928.1"
                     /translation="MAVKKKIINIRVYFDGDQDAKEVFAEVIAQKIRDTKTKDKLAIM
                     KDKQYNDYEFSNLKSA"
     gene            8964..10661
                     /locus_tag="NBH55_003029"
     CDS             8964..10661
                     /locus_tag="NBH55_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1510929.1"
                     /translation="MNNRYMSNNIERNATWRVGVYCRLSKDDELTGESASISNQRDIL
                     VNYCQSQGWQVVDVFQDDGYTGLNTDRPDLQRLLKACEKGLVNLVITKDQSRLGRNHV
                     QTGFLMEEFFPKHGVRYIALYDNVDTFAGDNEIAPFKNVLNEMYSRDISKKVHASYHL
                     QATKGKFTGVVPPLGYQKDPEEKGHLLIDDETAPIVRKIFQWAVDGHGINFISRELER
                     QQIPCPSWWHRKRGLRTYYTKWEKKDPENGKYVWDESYLKSLLMNPVYCGDMASQKRY
                     YRFKIGNQGDKAPEDWITVRDTHEAIIPQDVFDLVQAKMKGRKRQTEQGEYSMFAGLL
                     RCAECGGALTLKKTHTKDQHEVYTCSTYIHKGKAHCTQHRVDADDLYDAVLTRIQECA
                     KAVTGDGTELENRVKELCEEDTQGHRESLEKLVSKQKDRLETLDRLIAKLYDDLINDK
                     ITESVFDKMLEKTQKEQTDIKKELFQNESVLNTEEKLDAQSQQWIDDISEYADIKELD
                     ANLLNRLISKIVISEPQEKDGTENYCRTPETVTMEIHFNLKPIPELGTIERGSGSHK"
     gene            10686..10817
                     /locus_tag="NBH55_003030"
     CDS             10686..10817
                     /locus_tag="NBH55_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510930.1"
                     /translation="MLIFSRLISSKYFLIKDIKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            10909..11772
                     /locus_tag="NBH55_003031"
     CDS             10909..11772
                     /locus_tag="NBH55_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510931.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            11809..12345
                     /locus_tag="NBH55_003032"
     CDS             11809..12345
                     /locus_tag="NBH55_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HCD1510932.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            12359..12781
                     /locus_tag="NBH55_003033"
     CDS             12359..12781
                     /locus_tag="NBH55_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCD1510933.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            12711..15110
                     /locus_tag="NBH55_003034"
     CDS             12711..15110
                     /locus_tag="NBH55_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HCD1510934.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            15142..17133
                     /locus_tag="NBH55_003035"
     CDS             15142..17133
                     /locus_tag="NBH55_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCD1510935.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            17156..17407
                     /locus_tag="NBH55_003036"
     CDS             17156..17407
                     /locus_tag="NBH55_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HCD1510936.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            17397..18626
                     /locus_tag="NBH55_003037"
     CDS             17397..18626
                     /locus_tag="NBH55_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HCD1510937.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            18623..20704
                     /locus_tag="NBH55_003038"
     CDS             18623..20704
                     /locus_tag="NBH55_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCD1510938.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFANVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            20853..24773
                     /locus_tag="NBH55_003039"
     CDS             20853..24773
                     /locus_tag="NBH55_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HCD1510939.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            24774..25718
                     /locus_tag="NBH55_003040"
     CDS             24774..25718
                     /locus_tag="NBH55_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510940.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            25722..25925
                     /locus_tag="NBH55_003041"
     CDS             25722..25925
                     /locus_tag="NBH55_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HCD1510941.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(26225..26461)
                     /locus_tag="NBH55_003042"
     CDS             complement(26225..26461)
                     /locus_tag="NBH55_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510942.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(26644..27030)
                     /locus_tag="NBH55_003043"
     CDS             complement(26644..27030)
                     /locus_tag="NBH55_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HCD1510943.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(27012..28340)
                     /locus_tag="NBH55_003044"
     CDS             complement(27012..28340)
                     /locus_tag="NBH55_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCD1510944.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(28301..28630)
                     /gene="mobC"
                     /locus_tag="NBH55_003045"
     CDS             complement(28301..28630)
                     /gene="mobC"
                     /locus_tag="NBH55_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCD1510945.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(28888..29259)
                     /locus_tag="NBH55_003046"
     CDS             complement(28888..29259)
                     /locus_tag="NBH55_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1510946.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            29404..29589
                     /locus_tag="NBH55_003047"
     CDS             29404..29589
                     /locus_tag="NBH55_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1510947.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            29747..29914
                     /locus_tag="NBH55_003048"
     CDS             29747..29914
                     /locus_tag="NBH55_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510948.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            30532..31191
                     /gene="vanR"
                     /locus_tag="NBH55_003049"
     CDS             30532..31191
                     /gene="vanR"
                     /locus_tag="NBH55_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HCD1510949.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            31194..32537
                     /gene="vanS"
                     /locus_tag="NBH55_003050"
     CDS             31194..32537
                     /gene="vanS"
                     /locus_tag="NBH55_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCD1510950.1"
                     /translation="MERKGIFIKVFSYTIIVLLLVVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQNKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLGNIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            32708..33514
                     /gene="vanY"
                     /locus_tag="NBH55_003051"
     CDS             32708..33514
                     /gene="vanY"
                     /locus_tag="NBH55_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCD1510951.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLRWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            33532..34359
                     /gene="vanW"
                     /locus_tag="NBH55_003052"
     CDS             33532..34359
                     /gene="vanW"
                     /locus_tag="NBH55_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HCD1510952.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATIPEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            34356..35327
                     /locus_tag="NBH55_003053"
     CDS             34356..35327
                     /locus_tag="NBH55_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HCD1510953.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            35320..36348
                     /gene="vanB"
                     /locus_tag="NBH55_003054"
     CDS             35320..36348
                     /gene="vanB"
                     /locus_tag="NBH55_003054"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HCD1510954.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKFG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            36354..36962
                     /locus_tag="NBH55_003055"
     CDS             36354..36962
                     /locus_tag="NBH55_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HCD1510955.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(37028..37201)
                     /locus_tag="NBH55_003056"
     CDS             complement(37028..37201)
                     /locus_tag="NBH55_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510956.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            37549..37980
                     /locus_tag="NBH55_003057"
     CDS             37549..37980
                     /locus_tag="NBH55_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD1510957.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            37987..38220
                     /locus_tag="NBH55_003058"
     CDS             37987..38220
                     /locus_tag="NBH55_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510958.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            38644..38844
                     /locus_tag="NBH55_003059"
     CDS             38644..38844
                     /locus_tag="NBH55_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCD1510959.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            38928..40121
                     /locus_tag="NBH55_003060"
     CDS             38928..40121
                     /locus_tag="NBH55_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1510960.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            40293..40432
                     /locus_tag="NBH55_003061"
                     /pseudo
     CDS             40293..40432
                     /locus_tag="NBH55_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
BASE COUNT        11109 a         9422 c        10831 g         9070 t
ORIGIN      
        1 gaaatacagg gttttatagg gttaagtaac gaatacattg aaggtatttt tgtttctgcc
       61 gaaatgcaat ctcaaggtat aggtaaaatt ttgctaaact atgtaaaggg aaaaagaaac
      121 aaattaatct taaatgtata tcaaaaaaat acacgggcaa tatcctttta tcaaagagaa
      181 ggatttgaga ttcagtatag cggtttggat gaagctactg gagaaaaaga ctatgtaatg
      241 gcatggcagc agaaatagaa attcaagttt tagtgtttgg aaaattatga cttagtgtgc
      301 aagttgggag aaaatatggt aacggaaaaa gagaaaaaag catgtcgatt tattgccgaa
      361 tggctataag attgtgagac agtctctggt agatgatctg gagggcgaaa aaaaggtatg
      421 ttgggagaca gaaattttgc aggatgatga tagtggattt acttatgaaa tcagcaggtg
      481 tctgtatttc gatacctgta aggaacatgg ctatgcagag ttctgcaaag tattttgcaa
      541 ccatgactgg tatgcttatg gagtattaaa gcatcattcg agatttatca gaaaatcgac
      601 gattgccgaa gatggaactg tatgtcatga tacaatcgag aaagtaacag agaaacattt
      661 tttataaaac cgaaaaattg attatgggag gaaattgaaa atggcgaaag aagagagatt
      721 tgaaaaaata tattctcagg gagcattaac tactactgag atatgggtgg ataaggaaac
      781 tggtgtgaat tatttatatc atgcatgtgg gtattcaggt ggactgactc cattgttgga
      841 cagagatggg aaaccagtta tttctcaggc tataaataga tgaccttaga aacagcaact
      901 gttatggtaa gtaacagcat cgtcacaata tgtatatggt cgattggtgt gtgcggtaat
      961 atactatatt cagaaaaaca attcaggaga tttttatgag aagatcaggt tggacaaaat
     1021 ggttgatcgt gattccggca atgctaggca ttgtgttggt gacctatgtg cggtatacaa
     1081 cggatatctg gggatttggt gcatttatct gccagctgat tgcaattttg gaattggctt
     1141 atggtctgcg aattgcgatg ctggcacaga atagaaagaa gtcctatcgg ttaacaccgg
     1201 aagaacgaca tgagtatgca cggtatttat atgagaagca atatcaccgt taccctgctg
     1261 tagcgaatca gatgcttttg gttatggcac ggatgggtgt actccttaac aattatgaac
     1321 gggctgcaca ggagctggca gatattcgta tagataaatt caatccagca cagttgaagt
     1381 tgtattatta cctgaaagtg gttacagcaa tggcagcagg agatgcgaca gggattcaag
     1441 agagccagat gtgttacgca gggattccgg atactaaatg cacatatccg tcaaaagcag
     1501 agcttgctat gtggatagag cagagagcgg cagcgcagat ggcagaggca ctgaagaagg
     1561 cagtgccgga taaaaaagag catccgatac ggatcagtat cattactttg gttcttgcgt
     1621 atagtacctt cttctatgga ttgtggtatg gtataaaacg tgatatggga tatgaagtga
     1681 gatatcgttt cgcagaaata tcgattactg ttattacaat cagtctggcg gttcttttga
     1741 tctgggggat cgtgagttta tacagacaca attgccaggc attatccata caattcggta
     1801 aaaacaaagt ggcattgata attgtctatg gagctgctgc gatatttgca cttttgatta
     1861 ttggaatgag tggtctgagt gtagtgttcg gaatggatgg aacagaaacg gtgactggtt
     1921 gtgacagcaa atatacgtat ataactgtgc aggaagatta tggtggtgaa cggagataca
     1981 gaaccaataa tccatttgtt atgcagagta tggatggtct gtggtggtct tcggatacaa
     2041 tagatgaaga agataatcaa gaagaggata ctacagaatt accggatttg gatacagaga
     2101 attcaacaga ttcagaggat attcagagtg gaaataccat agaagattac gagacagaag
     2161 ggcagcatga tggtctgaca attcaaaatg caatgctggc agtttataat taccttcagg
     2221 agcagaatac acttcaaaat atggcgttca gttacacggc gaatgcaaag ggagaagtct
     2281 atgctgtcgt cagcgaaacg caggaaacaa agaacggtaa tacggtaaat gtgagatact
     2341 gtctgtacga taatggttcc aagacagatg cagatggtaa ttcctgcgaa gaactggtgt
     2401 tagaaaaagt atatccggat ggcaattacg agaccgaatt ggtagatttt tatctggtaa
     2461 atgcagatac gatgcaggtg acagacgaac agaagaatac gtggtaaaat aaattctggg
     2521 gaagtaggtg cattgtatga caatgagaga aaaaataaaa ctattatcgt aaatgtttca
     2581 agcggactgg gttcttttgg aagtatgcaa aaggactcca agacataaga ggtgaactgc
     2641 gctgatccag gtgccacggc aacagatttg aatgtcgacg gaggaaagca aacagtacag
     2701 gcgcaatcgt aaaacttgca atgattgata aaaccggact aacagtaact tttattagtc
     2761 gatttggaaa tatgccttgg taatcaacaa ttccggtttt actgactaag aaaatgagga
     2821 ggattcataa tgagtgagaa aacagagatt acttttatgc aaacaagact aatccggctt
     2881 gcttcagaag agtggcattt acctgttgaa cagattatcc acttgtttaa agaagccgat
     2941 gttcttggat atatagaaaa atgctatggg atttttcatt gtgaaggcga tgaagctgtg
     3001 cttgaagata ttactgaatt tttgcaaaga aaggggatag agattagtgc ttgagttaaa
     3061 agatggattt gttctctatc atggaagtta ttgtgaagta aaagagccag atcttgccaa
     3121 atgtgcaaag agaaaagatt ttggacaggg attttattta accacatcaa aggaacaggc
     3181 agaaagtttt ctgagaacct caatagccaa agccatcgca accggaacaa ttgaagaggg
     3241 gcagaagttt ggatatattt caacttttga attcaaactt tcagaaaatc ttgaaacgca
     3301 tatttttgaa aatgcagatg tggactggtt acattgtatc gccgctcacc gaaagaaaaa
     3361 aatgtttatt gaggttgaac gtgaaatggc aaaatatgat atcattgcag gaaagattgc
     3421 ggatgatgca acaaacgcta ctcttaccgc ttatcttgcg ggtgcttttg ggactgcagg
     3481 tgataaagta gcagacgatt tctgtatcag acagttgctg ccgaataaat tgaaagatca
     3541 atactgcttt aaaactgagg cagctattga gtgtctgaaa tttgtgaaag gtgagaagat
     3601 atggctgaag taacaattcc aaaggaaaaa atgaattata caatcgacct tctgatcact
     3661 atggtgacag atgaaatcgc agaagagaca ggcaaggaca gaaacgaggt attgacagat
     3721 tttttgtgtt caaagacagg aaaagcactg tatgatgaga agacaaagct gtggtgcact
     3781 ggtccggcat atatagctga actgtatatg gaagaattaa agaaatcctg atatgggatg
     3841 tgagtttaaa tttatacgac aatggtgaca aagactttca ttccaaggag caacaattaa
     3901 atatttcaaa aacgctgagg tcagcatttc ctaatcacag gacaacgacc tcagtttttt
     3961 atacccagga atcggcattc atgaataagc cggttctttt tgtttacaga aaaaagaagg
     4021 agtacaagaa aatgagcaga gcaaaaaatc caaagtatga atccatgtca ttggaggaac
     4081 tgaaggccaa catggaaaag agcagacagg aactggaggc agcaattcac cacaaaaaca
     4141 ttctggagca gcggaagaag ctggtagaac gcagggaacg ttcccaccgg ctgattgtca
     4201 aaggggcaga gtttgagaaa gtatttccgc tgtccagaga tctggaacag gaagacgtcc
     4261 aggacatgat gaatcaactg cagaacagca gttacaacaa gagtattgtg cggcaggctc
     4321 atatagccgc cttacacaaa gatcaacagc agattgcaga agcagtaaaa aagacagaga
     4381 agggagatga cagttaatgg cactgtatca tttttcagta aggaatgtat ccagagggaa
     4441 agggcagatg gtggtcgctt ccgcagccta tatttccggg cagaggattt ttgacagtta
     4501 ttacaacaag atacacgatt atacgagcaa gtcaggtgtg atatttacag aaatcctgac
     4561 accggagtat gtgccagagc gtctgactga cagggaaaca ctctggaacg aggtggagca
     4621 tgtggaacgg aacaacaagg cacagcttgc ctattcgttt gacattgcac tgcagaatga
     4681 attgacactt gaggaaaata tcagactggc aaaagagttc tgccaggagc agtttgtggc
     4741 acgtggcatg atcgtggatc tggcagtcca tgagggaaaa gcggaaaatg aagaagaacc
     4801 ggataatccc catttccatg tgcttgtccc aatccgtcct tttacagagg aaggaatctg
     4861 gggcaataag cagagaaggg aatatattct tgatgaggat ggaaaccggg tgaaggatgc
     4921 caaaggaaaa gatatgttca atgctgtttc tactaccgga tggaatgatc cggagctttt
     4981 aaaagaatgg cggcgggcat ggacagagaa agtaaatggg aaattcaggg agtgccacat
     5041 ggcatccaga atcgaccacc gttcctacaa agaacagggg attgacctga tcccgaccat
     5101 ccatgaggga tatgaagtcc gggcaatgga gaaaaaggga atcaaaacgg tgatcggaga
     5161 attgaaccgg gcaatccggc agtttaacca gatgtttatt tctctgaaag aatccattca
     5221 gtggatgaag acagcttatg aggaaatgaa agcagagctg gatcgcaggc agaacccgac
     5281 actgctggaa agcctgcagg attattatga taaaaagaca cagggcagac caccacttcc
     5341 caatttttat gcggagatga aacagagagg taagaatctc tccaatttac aggagtttgc
     5401 aaaatccatc agttatcttc agacacatca gatagaaacg atggatgact tgcaggaacg
     5461 gattgaggaa ctgaacggtg ttgtttctgt cagtaaaaaa gaaatttctg aaaagcggaa
     5521 acagttaaag gaacttgaga atctggaaaa gatggcagag gtaataaaaa caaatcagcc
     5581 attgattgat gagtataacc atttctattt tcagaaaaga cgggagagat attatcagca
     5641 gcataaaaaa gaaatcaatt attaccgcaa atgtgaacgg gaactgaaac aacatctgga
     5701 taaaaatgga aaagtgccaa ctgccagatg gaaacgggag aaagaagaac ttcaggcggg
     5761 gatcgaagag ctgaaagcag ataatcagcc gtaccaggag gaactaactt ttgtgaaaaa
     5821 ggtacagagc tgtgcggata ttgccagacg tgaccgggaa atggcagaag cagatacttc
     5881 cgggcgttca gaagaaaaaa gggagaagca ggtgaagttc ccgacatttt acacagttca
     5941 ggcgaaagag attcttgaag aaaacgggaa agcagagcaa cagccgttga accagtcgga
     6001 acagaatcca gaaaagaaga cctctcttct gagaaaactg gatgaaaaaa agaaagagtg
     6061 tgcagagcgt gatgcaaaac aacaggcagt gaagaaaaag cgaaactatg acatgagttt
     6121 ataacagtct gtcagcagga cagacgggaa aggatgagca aaatgcagta tacagaagaa
     6181 caaattataa gagcaaatca gacagatctt gtttcttttc tgagcgcaca gggagaacaa
     6241 ctagggaaaa gcggaaagga ataccgttgg aaaaagcatg acagtgtaac ggtatccgga
     6301 aaccggtggt acaggcacag tcaggggagg ggtggctatc cggtggattt tgtgatggaa
     6361 ttttacaatg ccacatttcc ggaagctgtc aagatgctaa ccggggagga aggcgaaggc
     6421 aggaacagta cctgccccgc cccgtcccct gacttccgat taccagagaa agaggaaaat
     6481 aatgatagaa tcatcagata tctgacagaa aacagaggga ttgaaaagaa tatggtggag
     6541 gaatggatcg gcagtgggga tatttatgaa gaaaagaagc accataatgt ggtgtttgtt
     6601 ggcagagatg cagacggtat tccaagatat gcccattgca ggggaactgg tgaaacaaaa
     6661 taccggggag atgtggctga atctgataaa tcttacggat tctgtcacag agggacggat
     6721 aatcagttgt ttgtgtttga agcagccatt gatctgcttt cttttatcca attatttccc
     6781 aaagactgga aaaagcggag ctacctgagt ctgggtggca tttccagtgc ggcactgatg
     6841 gcttttcttt ctgaacgccc tcagattact tcggttttcc tgtgcctgga taatgaccat
     6901 gcagggaatg aagccagtga gaaattggca atagaaatcc cggacggata cagtgtcatc
     6961 cgtctgaaac cgtcccgaaa ggactggaat gaaatcctct gtgataaaaa tgcagacagg
     7021 aagaaatcaa tcattgaaac agttacaatg aaagtaccgg agaaggaaga actggtgccg
     7081 atgttgtgtt atgaagacat tgaacagacc agtgtagaat ggctgtggtt tccctatctg
     7141 ccatttggaa aactgacgat tattcagggc aatccaggag agggaaaaac ttattttgcc
     7201 atgatgctga ccgcagcctg cacgaaccgt aagactttcc cgaacatgga agagattgaa
     7261 ccttttaatg tgatctatca gactgcagaa gacggtatgg gagatacgat taagccgagg
     7321 ctggtggaag caggagcaga cctgagtcgg gtgatggtca ttgatgatac agaggaagca
     7381 ttgacgttat ccgatgaccg tattgaaaaa gcaatccgtc agaatcaggt cagactttta
     7441 atcattgatc cggtacaggc ttttatcggt gccgatgtgg atatgaaccg ggcaaatgaa
     7501 gtccgtccgg tattccggaa actgggaatg atcgctgaaa agaccggttg tgcgattgtg
     7561 ctgatcggac atctgaataa atcttcaggg acacagagta cttaccgggg acttggttct
     7621 attgatatca tggcggcggt gagaagcctg ctttttatcg ggaaagtaaa gaaagacccg
     7681 acaacaagag tactgatcca tgagaaaagt tctcttgcac ctccaggtga gacgatggca
     7741 tttaaacttg gagatgaaga aggattccgc tgggtgggag cttatgagat ttccgcagat
     7801 gatcttctgg acggaaagga aggaaagccg acagaaacaa aactgcaacg gggaacgaaa
     7861 ctgatctatg aactgcttgc agacggaaat gcagtcacaa tccgggaact ggatgaaaag
     7921 gcaaaagcac agggaatctc ccagagaacc atgcgagaag ccagaagccg gatgaaagaa
     7981 gaactggatt atcggatgaa tgaaaagcag gaaaatacca ttcgattaaa aaaacaaggg
     8041 agaatgggtg acggcagaat tttggagtga acggcaggct ttatagatac cctatctgcc
     8101 gtctgccgtc tgccggtgca aggcaatcaa acggaaatag caggtatcca tacagaataa
     8161 ggattgctgt gaatctggaa caaaaatgaa ttgtaatatg gcggatataa tggtatgatt
     8221 atccggtaac agcaatgtta cggggaagca cctgtgacag ggtgtggctg cctcctgata
     8281 tttacagacc ggagcctttc ggggttctga gatgaaagaa aggaggtgcg tgatatgaca
     8341 gcttatgaga ttatctcaat attcttaggc gtactcagtt tactgatcgc ttttggtagc
     8401 ctgttaattg cgattattgc ctttcttgat cggaagaaca agaggaaata aaaatgccta
     8461 tcctgtctgc caacaggata ggcggtggtc gtaagaccat ttagtcaact caggcggcat
     8521 ccactttgga gtgggtgctt ttcctataac aaatgtacca cacattcctc ttgttttcaa
     8581 tgggaaaatt aaaatatgca gttcatctga cagggattta tccctgtctt tattatatcc
     8641 ccaatttctg aaatgttaat acaaaaattt taatatatgt aaccaacagt ctgggcagtt
     8701 aatctgtaaa aatggattag ctgctctttt tttgcaaaaa atggaggttt tacaatggca
     8761 gtgaagaaga aaattatcaa catcagagta tattttgacg gtgatcagga cgcaaaagaa
     8821 gtgtttgcag aggtgatcgc acagaaaata cgtgacacga aaacaaaaga taaacttgca
     8881 ataatgaaag ataagcagta taatgattat gagttcagta atctgaaatc tgcgtaggat
     8941 gtgcaaagta acggaggtat cagatgaaca acagatatat gagtaacaat atagaaagaa
     9001 atgcaacctg gagagttggt gtatactgcc gactttccaa agatgacgaa ttgaccggag
     9061 agagtgccag catctccaac cagagagaca tactggtgaa ttactgtcag tcacagggct
     9121 ggcaggtggt ggatgtgttt caggacgatg gttatacagg attgaacaca gaccgacctg
     9181 atttgcagag acttctcaaa gcctgtgaaa aaggactggt gaatcttgta attaccaaag
     9241 accagagccg actgggacga aaccatgtac agacaggatt tctgatggag gagtttttcc
     9301 caaagcatgg agtaaggtat atcgcattat atgataatgt ggataccttt gccggagaca
     9361 atgagattgc cccatttaag aacgtgctga acgagatgta ttccagagat atttccaaga
     9421 aagtccatgc gtcctatcac ctgcaggcaa ccaagggaaa gtttacaggt gttgttccac
     9481 cactgggata tcagaaagac ccggaggaaa agggacatct cctgattgac gacgagacag
     9541 caccgattgt ccggaaaatt ttccagtggg cggtggacgg tcatgggatt aactttatat
     9601 ccagagaact ggaacgccag cagattccct gcccgtcatg gtggcaccgg aaacggggac
     9661 tccgcaccta ttacacaaaa tgggaaaaga aagacccgga aaatgggaaa tatgtatggg
     9721 atgaatccta tctgaaatcc ctgctgatga atcccgttta ctgtggtgac atggcttccc
     9781 agaaaaggta ctaccgtttc aagattggca atcagggaga taaagctccg gaggactgga
     9841 tcacagtcag ggatacccac gaagcaatca ttccgcagga tgtatttgat ctggtacagg
     9901 caaagatgaa aggcaggaaa cgccagacag aacagggaga atacagcatg tttgccggat
     9961 tactccgctg tgcagagtgt ggcggtgcgt tgacgttgaa gaagacgcac accaaagacc
    10021 agcatgaagt ttatacctgt tccacttata tccataaggg gaaagcacat tgtacccagc
    10081 accgggtgga cgctgatgac ctgtatgatg cagtccttac cagaatccag gaatgtgcaa
    10141 aagcagttac cggagacgga acagaactgg aaaaccgggt aaaggagtta tgcgaggaag
    10201 atacacaggg acacagggaa agtctggaga agctggtatc aaagcagaaa gaccgcctgg
    10261 aaacgttaga ccggctgatt gcaaaactgt atgatgacct tatcaatgac aagattacag
    10321 aatctgtttt tgataagatg ctggagaaga cacagaagga gcagacagat atcaaaaagg
    10381 aacttttcca gaatgagagt gtgctgaata cggaagaaaa actggatgca cagtcccagc
    10441 agtggattga cgatattagc gagtatgcgg acatcaaaga actggatgcc aatcttctga
    10501 accggctgat cagtaaaatc gttatctcag aaccgcagga gaaagacgga acagaaaatt
    10561 actgccgtac tcctgaaacg gtcacaatgg aaattcattt caacttaaaa ccaataccgg
    10621 agcttggaac catcgaaaga ggttctggct cccataaata agaaaaatct atcaaaattt
    10681 aatctatttt aatcttttca aggctcattt cgagcaaata ttttctcata aaagacataa
    10741 aacagcttgt cgctggcggc ggcgtggccc tgatcggtgt cactctggtt cccctgctgt
    10801 ccgggctgct gggctgatcc tggcctttgt ccctgtccaa acccttaact aatcgcctcc
    10861 gcgtcctccc ggccagaccg ggagggcgcg ggaaaggagg tgctacccgt gattagtgaa
    10921 atcattgaag aatggataaa aggcatcctc atggacggta tcacgggcaa cctgtccggg
    10981 ctgttcgata cggtaaatgc caaagtcgga gaaatcgccg ccgacgtggg acgaaccccg
    11041 caggcatgga acagcggtat tttcaatatg ctgcacagcc tgtcggaaac cgtgatcgtc
    11101 cccatagccg gggccatact cgcccttgtc atgtgctatg agcttatcca gatgataacg
    11161 gaaaagaaca atatgcacga ctttgacagc tccatgttct tccgctggat attcaaatcc
    11221 gcgtttgcaa tcctgatcgt aacgaacaca tggaacatcg tcatgggcgt attcgacgcc
    11281 acccaaagcg tcgtcaatca aagcgccggt gtgattatcg ggcagacatc cattgatttt
    11341 aacaccctga taccagacct tgaaagccaa ttggaggcta tgagcgtggg cgctcttttg
    11401 gggctgtggt tccaaaccct tgttgtgggc ctcaccatga atatcctttc catctgcatt
    11461 ttccttgtga cctatggccg catggtggaa atctatgttg tcacgtcgct ggggcctatc
    11521 ccccttgcca caatgggcaa cgcggaatgg cgcggcatgg gccaagggta tctgaaatcc
    11581 ctgctggcgc tgggcttcca agccttttta atcatggttg tggtgggtat ctacgcggtg
    11641 ctgatacaga atatcggttc cgcttccgac atatccggcg caatctgggg ctgtatgggc
    11701 tatacggtgc tgctgtgctt ctgcctgttc aagaccggca gtatatcaaa ggccgtattc
    11761 ggggcgcatt gatcccaaaa tccaagcata cacaggagga acttgtttat ttcaacgtac
    11821 aacatcattt acgctgaccc gccgtggcgg tatgactgca aaaacgtaca gggcgcagcg
    11881 gaaaatcact attccaccat gagcattgac gagctgtgcg ccctgccggt ggaacggctg
    11941 gcgtccaaag actgcctgct gtttttgtgg gccacattcc ccatgctgcc ggaagctctg
    12001 cggttaatca aagcgtgggg cttttcattc aagaccgtgg cttttgtatg gctgaaacag
    12061 aaccgtaaaa gccttacatg gttttacggg ctgggccgct ggacaagggg aaacgcggaa
    12121 atctgcctgc tggcaacaca cggcaagccg aagcgtcgtt ctgcctctgt ccatcagttt
    12181 attatcagcc cgattgaaca acacagcaaa aagccggatg tgacccgtga aaaaatcgta
    12241 gagctggcgg gcgatttgcc ccgcgtggag ctgtttgcca gacagaaaac ccccggctgg
    12301 gacgtgtggg ggaacgaggt ggaatgtgac cttatcctga cgtgaaagga ggtgccaaat
    12361 ggcttatgta accgtcccca aggacttaac ccatgtgaaa agcaaggttg tgtttgggct
    12421 taccaaacgg caactgatct gtttttccgg ggcgctggtg atcggcgtcc ccttatattt
    12481 tctcacccgt gactatctta ccaacagcgc ggcggccctg ctcatggtat ttgccatgct
    12541 gccgggcttt ttgcttgcca tgtacgagcg tcacggccag ccccttgaag tggtgattat
    12601 gcagatcatc cagtgctgct tcatccagcc aaaggagcgt ccctatcaga ccgacaacac
    12661 ctacaccgcc cttgtgcggc aataccagat ggaacaggag gtaaaggcca ttgtccaaaa
    12721 aggaaatccc aagagaaacc gaaaaaacaa agctgacccg cgcccagcga aaagaaattg
    12781 acgcggtaat ccgtaaatat aagggcgacg gcaggcccca cacggcgcag cagagcatcc
    12841 cctacgaggt catgtacccg gacggcgtgt gccgggtatc gcccggcgtc ttttccaagt
    12901 gcattgagtt tgcggacatc agctatcagc ttgcccagcc ggacacgcag acggctatct
    12961 ttgaaaaact gtgcgacctg tataactatg tggacgcctc cattcatatc cagttttctt
    13021 tcctcaatcg gaaagttgac ccggtgcagt acgccaagag ctttgagatc gcgccgcagg
    13081 gggacgactt cgacgacatc cgggccgagt acaccggcat tctgcaaaag cagcttgcaa
    13141 acggcaacaa cggcatggta aagaccaaat acctgacatt caccatcgag gccgaaagcg
    13201 tgaaagcggc gcgagccaga ttgaagcgca tcggctttga cctgttaggc tatttcaaga
    13261 gcatgggggc cgtagcccat gtgatggacg gctgggaacg gctgaacctg ctgcatggcg
    13321 tgtaccaccc ggacggcgag atattcaatt ttgactggaa gtggctggcc ccgtccggcc
    13381 tctccaccaa ggattttatt gccccgtcct ccctgtgctt cggcaatgca aagacattcg
    13441 gcatgggcgg caaatacggc gcggtaagtt tcctgcaaat cctgtccccg gagctgtcgg
    13501 acgatatgct ggccgacttc ctcaatacgg aaagcggtgt tttagtcaat ctccatgtgc
    13561 aggccattga acagaccaag gcgatcaaga ccatcaagcg caaaattacc gaccttgacg
    13621 ctatgaagat tgcggagcag aaaaaggccg tccgcagcgg ttacgatatg gacattcttc
    13681 ccagcgacct tgccacctac ggcgaggacg ccaagaagct gctgacaaag ttgcagaccc
    13741 gcaatgaacg gctgttccag ttgacgttcc ttgtcctcaa cgtggccgac accaagcaaa
    13801 agctgaataa cgacgtgttc caagccgcag gcgtggccca aaagcataac tgtccccttg
    13861 tccggctgga ctaccagcag gaacagggcc ttgcaagcag cctgccgctg ggcgtcaatc
    13921 agatcaagat acagcgcagc cttacgactt ccagcgtggc cgtgttcgtc ccctttgtga
    13981 cgcaggagct ttttcagggc ggcgcggcca tgtattacgg catcaacgcc aagtcccgca
    14041 acatgattat gctggacagg aagcaggccc gttgccccaa tgccttgaag ctcggcaccc
    14101 ccggcagcgg caaatccatg agctgtaaat ccgagatcgt cagtgtgttc ttaacgacgc
    14161 ctgacgatat ttttatttca gacccggagg ccgaatatta cccccttgtc aaacggctcc
    14221 acggacaggt aatccggctg tcgcctacca gcaaggactt tgtgaacccg ctggacatca
    14281 acctgaatta ttcggaggac gataacccgc tggccctgaa atccgacttt gtgctgtcgt
    14341 tctgtgagct tgtcatgggc ggcaagaacg ggctggaagc cattgaaaag accgtgatag
    14401 accgggctgt gcgcgtgatc taccggccct atctggcaga cccccgcccg gagaatatgc
    14461 cgatcctgtc cgacctccac aaagcgttgt tagatcagca tgtcccggag gctgaccggg
    14521 tggcgcaggc ccttgacctg tatgtgtccg gctccctgaa cgtatttaac caccgtacca
    14581 acgtggacat cggcaaccgg cttgtgtcct ttgacattaa ggagctgggc aagcagctca
    14641 aaaagctggg gatgctcatt gtccaagacc agatatgggg gcgcgtcacg gccaaccgaa
    14701 gccaaggaaa ggccacatgg tattttgccg acgagttcca tttgcttttg aaagaggaac
    14761 agacggcggc ctattctgcg gagatttgga agcggtttag gaaatggggc ggcattccaa
    14821 caggtgccac ccagaacgtc aaagacctgc tttcctcccc ggaaattgaa aatatccttg
    14881 aaaacagcga cttcattacg ctgctcaatc aggcgtccgg ggatagaaaa atccttgcgg
    14941 aacgcttgaa tttgtccacc gaacagcaga agtacatcga caattccgaa cccggcgagg
    15001 gccttttgat ctttgaaaac gtggtgctgc cctttaccaa ccccatcccg cacaacaccc
    15061 agctttacaa gattatgacg acccgcttaa acgaggtggc gggagtatga cccggcgacg
    15121 ccacaaagga ggtgaaccac catgaaagag ctgaaagcca aggcgaaagt gacgcaggcc
    15181 atgacccgcg acgggctgac gctggaaaat caggccaccg gcgaggtgga gaacatttcc
    15241 agccgggagg ccgaacagga tttgtccgcg ccggggccgg gcggcaccgc cgataagctg
    15301 ctggaacggg ccgacgccat ccatgacggc cataaggcca agaaagccgc gaagcaggcc
    15361 ggggctgtgg ccgccgaggg ggacgctgcc ctgcaccggc catccgcccg gctccaactg
    15421 tcggaggaag aacgggccga cccggattta cagccctata tccgaaaatc ggacaaacgg
    15481 gccgataagc tggataaggc ccgcgccgcc atccccaaga agcgcgtccc ggtcaaggaa
    15541 accgtctttg acgaggcaag cggcaaagca aagaccgtcc tgcactttga caaacaggac
    15601 aaggggcctc cctccctgaa acccaatccg gcaggccgtc ctgtttccga ggtttttcta
    15661 ttcggccacg ggaaaatcca tgaagtcgag catgaaaacg tgggtgtgga gggcggccat
    15721 aagggggagg aacttgccga gcggcaggcc ggaaaagcca tccgcagcac catccggcac
    15781 cacaggctga aaccctactg ggacgcggaa aaggccgagc gcaggctatt gtctgccaac
    15841 gcggagtatt tctaccagaa atctttgcgg gataacccgg agatcgcaca ggcggccagc
    15901 aaccccattt cccggttctg gcagaaacag cagatcaagc gccagtacgc caaggccgcc
    15961 cggacagcgg gccagaccgc aggcgcggcg gcacagggcg cggccaagac agccggggcc
    16021 tccacgaaag cggccaaggc cgcagccaaa gaaagtgaac gggcggcctc ctttgtggcc
    16081 cgccactgga aaggggcgct ggccgtcctt gtgttggggc ttttgatgct gcttatcatc
    16141 ggcggcttac agtcctgcac cgccatgttc ggcagcgccg gaagcggcat cacggcgtcc
    16201 tcttactttt ccgaggatag cgatatgttg gccgccgagg acgcctacgc ggagctggaa
    16261 gccgagctgc aagactatct ggacaactac gaggccaccc atgactatga cgaataccat
    16321 tttgaccttg acgagatcag ccatgacccc tatgtgctga tttccattct gtcggcgctc
    16381 catgacggga tatttaccat cggggaggta cagggtgatt tgtccatgct ctttgacaag
    16441 caatatatcc tgacagaaaa cgtcgtggta gaaacccgtt accgcaccga aaccgataca
    16501 tggacggacg cggagggcaa cacccataca gacacctacc aagtgccgta tgactattac
    16561 atctgctacg tcacgctgga aaacttcgac ctgtcccatg tgccagtcta tatcatggac
    16621 gaggaaacgc taagtttcta ttccctgtat atgtccacgc taggaaaccg gcctgatctg
    16681 ttttccggca gccagtaccc caacgcctcc accctgacgc cgccgaccta ctacgacgta
    16741 cccccggagg ctttggagga tgccaccttt gccgccatgc tcaaagaggc ggaaaaatat
    16801 atcggttatc cctatgtgtg gggcggcagc tctcccagca ccagctttga ttgcagcggc
    16861 ttcatttcgt gggtaatcaa tcatagtgga tggaacgtgg gacggctggg ggcgcagggc
    16921 ctatataaca tctgcacccc tgtttccagt tcgcaggcca agcccggcga ccttgtattt
    16981 ttcaagggaa cctacgacac ccccggcgtg tcccacgtcg gcctgtatgt gggcaacaat
    17041 gtgatgctgc attgcgggaa cccgatttcc tacacgaacc ttacttcaaa ctactggcaa
    17101 tcccattttt acagttacgg gcgtttacct taacgccaga aaggagcttt caattatggc
    17161 aaccaaactt gaccgcatcg aaaaggacat ccagaaaacc aagtctaaga tcgcggagtt
    17221 tcagaaacag cttcgggagc tggaaacgca gaaaaccgaa caggagaatt tgcagatcat
    17281 ccagcttgtg cgcggcatga acatgaagcc ggaggaattt gccgcgttct tacgcagcgg
    17341 cgcaatgcag gccgcccctg ccgcaacccc ttaccatgaa caggaggaca atgcccatga
    17401 agaataagac cagacggatt tcccgtattc tccccgcact tctggccgtg atgctctgcg
    17461 tcacggcctt tcccgttacc gcccttgcag gcggcaatga cccggcaccg gccccgctgc
    17521 cggaggctac aaccgaaccc gctacgggcg gtttggagcc tgaaacagac gaaaccggca
    17581 ccgccacgga cgggacagaa accgaggccg aaaaagatac cggccctctg accggcaagg
    17641 acatcaccga cctgttttcc gcgctgttcg gttccaaggt gagcattgcc gccacggacg
    17701 acggcatcca gatcaccacc ggcaccgaca aaaaagaacc tgaacagacc ggcaccgtca
    17761 ccaccaacgg cggcaggctg aatgtccgca ccggcgcggg gcttgacaag accgcgttca
    17821 cccagcttcc caatgggacg acggttgagg tagtcggcac agacggcgac tggattaaaa
    17881 ttctcttgcc ggagcgcatc ggctatgtcc attccgacta tatgaccgtc agcgaaaagg
    17941 aagtagcggc taccggcgag ggcggttttt ccctctccat tgacccggag gaaattgctt
    18001 ctctgctgga actgttcaat ggcggcgggg ccagcgccgc cctgtccccg gacgggaacc
    18061 tgtcactgat tgacgacatc ggcagcgccg cgaaaaatgg caagcagttt atcaccgtgg
    18121 aaaccaaaaa cggcaatgtg ttttatctga ttattgaccg ggacgacgag ggaaaagaaa
    18181 ctgtccattt cttaaatcag gtggatgaag ccgatctgct gacgctgatg gaggacgggg
    18241 aaaccgtcac ccccgctgct gtctgctcct gtaccacgaa atgcaaggcc ggggctgtga
    18301 acacgaattg cccggtatgt aaaaccaacc tgaccgagtg cagcggcccg gagccgcagg
    18361 aaccccagcc ggaggaaccc gaagccccgg aggaagaacc caagggcggc gcgggcggcc
    18421 tgatcgtgtt cctgctggtg gcgctggcgg gcggcggtgc ggccctttac tatttcaagt
    18481 tcaaaaagcc caaggctgaa accaccggca gcgacgattt gggcgagtat gattttgggg
    18541 aggacgagga tttggaggac gaggaacccg aaaccgagtt tgacccggaa gctgattttg
    18601 tgagtgaaca ggaggacgag gaatgacata taaacttgta atcgcggaaa aacccagcgt
    18661 ggcccgcagc attgcgggcg tgatcggggc cacggagaaa cacgacggct atttggaggg
    18721 gaacggctat ctggtgagct ggtgcatcgg acatctggtg tcctttgccg acgccggacg
    18781 ctatgacgag cgtttcaaaa aatggcgcta tgaggatttg cccattctcc cggaaacatg
    18841 gcagtacatc gtccccgacg acaagaaaca gcagtttgac gttctctgtt cgctgatgca
    18901 gcgccccgac gtgaccggcc ttgtgtgcgc caccgacgcc gggcgcgagg gggaattgat
    18961 tttccgtttc gtctaccaga tggcgggatg ccaaaagccc tttgagcgcc tttggatttc
    19021 ctctatggag gacagtgcta ttaaggacgg ctttgcccac ctgaaaccgg gcggcgacta
    19081 tgatccgctg tatcaatcgg ccctgtgccg ggccaaggcc gactggctga tcggcattaa
    19141 cgccacccgg cttttttccg tcctgtacca caagaacctg actgtgggcc gcgtccagac
    19201 gccgacctta aaaatgctgg tggacaggga cgccaagatt accgatttca agaaagagaa
    19261 atatcacatc gtccatatca cggcgggcgg cgcggacgcg gtaagcagcc gcttttccga
    19321 cgctgccgag gcaaacaccg tcaaggcggc ttgtgcaaaa gcgcaggccg tttgcgtttc
    19381 cgtgacgcgg gagaaaaaga cggaacagcc gcccaagctc tatgacctta ccaccttgca
    19441 gcgggaggct aaccggcttt tcggctatac cgccaagcag acccttgact atgcccaggc
    19501 actgtatcta aagaagctgc tgacctatcc gagaacggat agtcaatatc ttaccgacga
    19561 tatgctgccc gccgtggaaa gccttgtgtc cggcctgtgg gagctggtgc cgtttgccaa
    19621 gggcctgaac ctttccccgc agtttgaccg catcctgaac agcaaaaagg tgtccgacca
    19681 ccacgccatt atccccaccg ctgaatttgt gaagcagggc tttggcactc tggccgagag
    19741 tgaaaaaaag ctgctgtccc tgatctgctg caagctctta tgtgccgtgg ccgggccgca
    19801 tatctatgag gccgtcaccg ccacatttac ctgtgccggg caggagttta ccgccaaggg
    19861 caagaccgtc ctttcccccg gctggaaaga gattgaacgc cggttccgtt cttccttaaa
    19921 gaccgacgcc gacgaggacg ccgaggcggt gcgggagctg ccggagcttt gcgagggcca
    19981 gacctttgca aacgtggccg cctctgtcac cgagcatttc accacgccgc ccaagcccta
    20041 caccgaagat acgcttttgt ccgctatgga acgggccggg gccgaggata tgccggagga
    20101 tgccgagcgc aagggattag gcacaccggc cacccgcgcc gccattctgg aaaagctggt
    20161 gcagatgggc tttgtccaga gaaaaggcaa gcagctcatt cccaccaagg acggcatcaa
    20221 tctggctgtg gtgctgccgg aggcgcttac ttctccccag cttactgccg aatgggaaaa
    20281 ccggctgacc gagatcgcca aggggcaggc cgatcctgac gaatttatgg acggcatcga
    20341 gacgcaggcg cgggagctgg tgcagaccta ttcctgtatc agtgaggaaa agcagaaatt
    20401 gtttcaggcc gagcgtgttc ctatcgggac gtgtccccgc tgtggtgagg ctgtctatga
    20461 gggcaagaaa aactactact gcggaaaccg ggcctgccag ttcgtcatgt ggaagaatga
    20521 ccgatttttt gaggaacgca agaaagcgtt caccccgaag atcgccgctg cgctgctcaa
    20581 aaccggcaaa gtcaaggtaa agggcctcta ttccgtaaag accggcaaaa cctacgacgg
    20641 gacggtgctt ttggctgata ccgggggcaa gtacgtcaac taccgcattg aacacaggag
    20701 ctaaaggaca gtaacacagc aagcataggg agtgtggcta cactcccggt aaaatccccc
    20761 ggccgcgtcc ctgccgggcc ggggattttt gcttgttggg gaagtcccca accccctatt
    20821 ttttgaaaca gaacagagaa aggagtgacc gcgtgaacga agtattttct atccaagtcc
    20881 ataaccggca gtttttggaa cagggcaaac aaggcgtctg gctggatttg cccaccacaa
    20941 ccgaaacgct gcaagccgcc ctgcgggatg ttggcattac tgccgacaat ccgcaggatt
    21001 tttttatcaa cgggtattcc tgcccggagg acagacacct tgccattccc tatgacatgg
    21061 tgctggccgc cgacgtggac gagctgaatt tccttgccgc ccggctgggc cagcttgacg
    21121 ccgccgagat cgcagagctg aacgccgccc tgcaaaatcc ccgtggcggc tttgagaata
    21181 tcggccagat catcgactac accgaaaatg tggattacta tgtgcatctt cccgacgtta
    21241 ggggggatgg acagttgggc gactactacc tgaaccgttc cggcatggtg gatatgccgg
    21301 aggaatggaa agccgggatt gatacggcgc agtttggacg ccacattgcc cagcaggaac
    21361 aaggcgtttt tacccagtac ggctatctgg tgagaagcgg cgacgaatgg cagcgcgtcc
    21421 atgagggcca gccggtgccg gaggaatacc gggtgatgca ctacccccag ccggaagctg
    21481 accgggagga accgcaggcc cagcagccgg aggcatccga aagccccgac aaagcgccgc
    21541 ccccggttat cccgatcatg ctggacggca tgaacagcaa agaacgcatg aaagtgatta
    21601 ctgaccgtct ggaaaccggc gtacaggagc tttttgaaag tagccgctat atggcttact
    21661 tatccgctat gtccaagatg cacgattaca gtctgaacaa tacgctgctg atcgccatgc
    21721 agggcggttc gattgtggcg ggctataaca ggtggcagga tgaatttgac cgccatgtga
    21781 agcggggcga gaaaggcatt aagatactgg ccccggcccc gttcaaggtt aagcagcaga
    21841 tggagaaaat cgacccggcc acccagcagc ccgtgatcgg cgcggacggc aagcccgtga
    21901 ccgaagaaca ggaagtgacg gttccggcct ttaaggtggt gacagtcttt gacatcagcc
    21961 agaccgaggg caaggatttt cccatgccct atatcagtga acttaccggc gacgtgcagc
    22021 agtatcagga tttctttgcc gcgctggaac ggacatcacc ctatcccata agcattgagc
    22081 cgatttcaga cggggccaag ggccgctgct tctacgcaaa acagcgcatt gccatcaacg
    22141 agggcatgag cgaattgcag accctaaaga ccgccatcca tgagattgcc catgccaagc
    22201 tccacgccat tgaccgggag gcccccgccg aggaacaggc ggcccggcca gaccgcagca
    22261 cccgcgaggt acaggccgaa agcgtggcct ataccgtctg ccagtattac gggctggaca
    22321 cgtcggacta ttcttttggc tatgtggccg attggagcag cggcagggag ttatccgagc
    22381 tgaaagcctc ccttgaaacc atccgggcca cggccagcga attgataacc gagatcgacg
    22441 gccattttgc ccagcttcag caggagcgcg aggcccggca ggaacaggag gccgtcacgg
    22501 ttgacgttac cccggaggca gcggccccgg cgcaggccga ggaagaacag gccccggccc
    22561 ttgatccggc agccgagccg cttgtgactg tgctatggag cgaaagcccc cagctaaagg
    22621 acggccagca aatgccgttg catgaggccg acgcgctgtt tgcccgtctg gatgcagcat
    22681 atccggcaga ggcaggctat gacaaaaccg cgttccgcat tgactgtatc tttcagggcg
    22741 agccggacag ctacgagggg cggcaggatt ttggagatag ggacggttcc ctgatcgaac
    22801 atatccagag ctaccaccaa ttttacgcgc aggacgaaaa ctggaaaaag catgtaatcg
    22861 ctacaaccgg catagagggc tgggagcagg acaaggccca tcgggatatg ctgttaggcg
    22921 agtttgtccc ctatatgaag ctgcattgca gccttgccca aatggaacag acggcgcagg
    22981 gccgcttaca gtccggggaa accctgacgc cggaggaaac ggcctatttg ggggcgcttc
    23041 tctcttatgt gcaggagtgc cgccccatgc tcaatcaggg ccagtatcag ttgccggagc
    23101 cgccgaaact ggctgacttc gaccagagct tgcaggacta caaagcacag gtacaggccg
    23161 agatcgcgca ggaggccgcc gcagccggta tgaccgtgga ggaatacgcg gcggctggct
    23221 atgaagcacc ccaacaggac accttttcca tctaccagct tcgaggcggg gaggaaaccc
    23281 gcgactatcg gtttgagcca tacgagcgtt tacaggccgc agggctggcc gtggacagtg
    23341 ccaactatga ccttgtttat acggcaccgc tggataaaag cacgtctttg gaggatattt
    23401 accgcacatt caacatcgac cacccggcag attttagggg acacagcctt tccgtttccg
    23461 acgtggtggt gctgcaccag aatggacagg acaccgccca ctatgtagac agcttcggct
    23521 atatggaagt gccggagttt ttgcgggaaa atcccctgcg taccgccgaa ctgtctacgg
    23581 agcagaacga aaacatgatt gacggcatta tcaacaatac gccgcccgaa ccgcagccgg
    23641 aggaacccga acagctatct tttgaggatg tggcagttgc ttccggccag acacaggagg
    23701 aacccccgga ggctgcgccc ccgcaaacgg ctgtccgcta ttatcccatc aatgagggag
    23761 ccgcccgccg cgccaaggaa gccatgagct tttctgacta ccggcccggc agcgccacgg
    23821 cggaataccg gcactatgtc gatcaggccg tggaaattgc ggagcgccag aaaaagcgtg
    23881 tggagccaga gtatcacgaa aagatagaca gcctccttga cacctacgcc cgcaagctgg
    23941 ccgagaacat gaataagggc tatgagatta ccgcccgcgt cccctctgtc ctgattgccg
    24001 ggccgtccaa tttcccggta cgggcgaaag aaaagcagaa cgccgccagt gaccgcaaca
    24061 tggaggaatt tcagtacatt cagggcttgc ttgacaaaat ccgcagcacc ggcatgggtg
    24121 gtatcagcgc cgacgatccg caggccgttt ccaagctgga aaagaaactg gaaaagctgg
    24181 aagcgtcgca ggagttgatg aaggccgtca atgcctacta ccgcaagcat gggacgctgg
    24241 acggatgccc ccatctgacc gaaagaggca ttgaaaacct gaaagcggat atggcgtcgg
    24301 gctggcacta tgagaaaaaa ccgttccagt cctggcagct ctccaacaat aacgccgaga
    24361 tacgccggtt aaagggccgc attgaggaat tgacccgcca aaaggaggcg gcctatgtgg
    24421 gatgggagtt tgacggcggc actgtggaga tcaaccgggt ggccaaccgc ctgcaaatct
    24481 tctttgaggg gaagccggat gctgctgtac gcgacgagct gaaaagcaac ggtttccgct
    24541 ggtcgcccaa ggccggggca tggcagcggc agttaaacga caccacaatc cgcgtcgctg
    24601 accgtatcaa gtgcatccag cccctttccg gcgaaaagcc cagcgcctta cagcttgcgg
    24661 cccgcaggga gaaagaaaag ccctctatcc gggcgcagct caacgcggcc aagaccgaac
    24721 aggccaagca gccaccggcg cgggaaaaat caaagggatt ggaggtagag taaatgcaga
    24781 agtttgaaaa tgtggacgtt ctggccgcgc tggaacagat catgcggcag aacaccgcgt
    24841 tttatcaaag tgactttggc attgacaaag acattatcag gcgggatgcc gccagcgatc
    24901 aggccgcaga caagaccctg ctctggatgt cccggccctc cgggacgtac tgtttccggg
    24961 aacgtgatgt tttcctgaaa gacacccgac agtacaacac atggaaattt tacggggagc
    25021 agacccgtga caagatactg gcctacgcgg tggagctgac cggcacacag ggcggcacta
    25081 tccggggcaa tctctatgag ctggactatc cgcagcattt ccggcacgtc attgaaaccg
    25141 cccagccggt gagtgtgaac cggctctttt atgagcatgg cacccgtgac atcccggaga
    25201 gccagcgttt tgacggaagc cctgaccggg tattgggaaa ctttttgtac tatgaggccc
    25261 agccccatga cccggctgtg ctgcaggagg cgctacggac ggagcagcac agccgggggc
    25321 gggccgtccc cggcgatttc aaagtccacg tcaccgcttt gcatgacagc cggattgaaa
    25381 ccgaggcccg gcgtattgtg gacagcctga aagatctgtc cgcgcctaac agccccaata
    25441 aaacgcattt catggtggag atctccccgc actttgcggc gattgcttcc accaaggata
    25501 atgaccggct ctttgccatg ctgccctata aatccctgtg ttttaccggg atgaaaggca
    25561 ggcatggcgt gtatgccgtg attaaccggg acgagcgccg ggatgtgagt attcgcaggc
    25621 cccggccctc tatccgcaag cagctatccg acagcaaaca ggccgccagc ccaaaaaagg
    25681 cggcggccag aaccaaaaag aatgaattgg aggtataagg aatgatgcac tttaccgtgg
    25741 aggaagaaaa cctgatctgt atgtaccaca atgcagaccg gcgcagaacg ataaacaggc
    25801 tcaccgccgt catgccggtt atggatgggg atatgcgggc gcaggcccag cagacccttt
    25861 ccaagctgga acgcatgacg gatgccgact atgacggcca gaagttccac ttcacagacg
    25921 aataacgaac agcagacgcc gggcgcggcg gccatgtggt cgcttgcgtc cggcgtcttt
    25981 tctttttgcc tattcctacg catttagaac ccggtattgt cggggatagg atgaagtatt
    26041 cgcttgcccc tgcaaacatg cgggagaagc cttgcagcgg gcggttttca atcccttaaa
    26101 tgcgtaggtt gcagctataa ttagacccct gaaacagata tagccgcgca gcgggtgtat
    26161 ctgtttcagg ggtgtgcagg tgatagccgc aaagcggcgt aagagggtgc agccctcttg
    26221 ccgtctatct ctgttccagc gggaagctcg gcaatgcttc cagcagtttc agggaaatgc
    26281 gctgccgtat atcttcgtca atgactttct tctttttgcc gtttgcctgt ctgatctcca
    26341 ccgtggaaag ctcgttgatg tagtcagcat aatgggccaa cactttttcc gtggcccacc
    26401 tctcaccggc aacggcggct ttgatcgttt catagtccag cagcttttct tgtccgtcca
    26461 tcctgatacc cccgtcagac tgattttttc tatgatacca tattcggaag catggaacca
    26521 gagaaaacgg cgcttttctg ctggtaaatt ctacgcattt agaacagcac ttcggcgggg
    26581 ataggataaa acacccacat accccgctga ccatgccgga aaagccttga aatataaggg
    26641 cttttacagc tctaagtgcg taggtttcca gtatcttttc cgctggcgct cggcctcttt
    26701 gcggcggcgc atccgtttgg agcaggccgc gcagtatttg gcccggttag acttcggcgt
    26761 gaacgtggcc ccgcactcac agcagcgttt cacttcgtcc cggtctttgg tgatctcggc
    26821 atacagggcc gcatccagcg ggaggacagc ggccttaaac cagcggcaca gaagcgaata
    26881 ggaaatgctc tgtacgcaaa tacattcttc gccgtcgtcc agcaacaggc agttgccgtc
    26941 acaatagttg cagcactcat gcgtcagctt ccgggccttg cgatattgag ggtaattcat
    27001 tcgtggaata ctcatagctc ttgcgtccat tcctttcccg gcgtcatacg caaaatgccg
    27061 tccacattct gcttgatgat gccgtattcc tgcatctgct ttttcgcttc gccgtacttc
    27121 tcatacagcc gttccttttc ctcggccaat cgcttgtact cggctttcag ggcgtacaga
    27181 tcggggagct ttttcacacc ctgtcccttt agggcctttg ccgcagcttc atacaaaatc
    27241 aacgtgcttt catgctcacg ccggaatttt gccttatccg ctgcattgcg gtattccata
    27301 gccacgggcc agagctgctt gtaggtagaa atattcttta tcagcaccgc catatccgca
    27361 agccggtgtt ccatagcttt gagggtagcg gcggcctcgt cgctggcggc ggtgatctcg
    27421 gccactttgc tttccaaatc cgggtaggtg tctatcccat gttcagtcag gaaattcaaa
    27481 gtctttgcgg cctgcttcaa attgtggagc ttcgcccacc tctcatagcc tgccgactgc
    27541 tgggccttaa tgttgttttc cagatcaatc cgcaaagaaa tccccttgcg ctctttgggc
    27601 gctctggtga taacgcggcc tttgatacgc tctttgatgg cttcctcggt gtaggcttcc
    27661 cccaatgtct tgcagcgggt aaagcgttcc tgtccgggag cgcggaaaga aataaacttt
    27721 cccggcttga tctcatagcc ctgcgcctgc atgagccgca ggaaatcatc aaaatctttt
    27781 gcctgcggga tagcggcgtc aatggctgct ttgagcttcg ctttccagct cgtccctttc
    27841 cgctgggcgt cccactcggc atagctcttg cccttactct ggccgggctt caccacggac
    27901 aatccgtttt ccttgcacag cctgtcgctg gtgcgccgga tataggcata gctctgtttg
    27961 ttggaaatgt acttgcgctg atttaccata tccacggcac agaaaataat gtgattgtgg
    28021 acgtggccct tatcaatatg ggtagtcagc acatagggat atttcccttg cagcacttcc
    28081 tcggcaagct gcttcccgat ctcatgggcc tgttccggcg tggcttcgcc gggggcaaag
    28141 gattgtatca ggtggtggcc cagattattg cccttttggt atgcctgcgc cagcagcatt
    28201 tcaaattcaa tgtccgctgt ttcataagag caggcaaagg aggccaccag cagcttgccg
    28261 tcagttttgt ccgggttctc gatgtagtca agggcttttt tcagcgttga cttgatagga
    28321 tgtatctttg taactgccat atctgcgcca gcgccccctt tatatcctcg atgtcctgtg
    28381 cgtacaccgg ccccataccg ttcagcctct ttgcaatctg attgacgttg acgccgatct
    28441 tctgtatctc ggcggtctgc gcccggatgt cgctggtgtc catgttgacg atatagccgt
    28501 caatgagcat cttccgggcg taggctgaaa agttcttcgt atgcagcagc gccattttct
    28561 gttcaatcat agcccgttcc tccggcgtca cagggacgcg cagcacgatt tttcgctttc
    28621 tgtttgccat gcttcaccgt cctttccatt caggggtttg gggatttccc caacaagcaa
    28681 aacggacgcg gcccggcagg gacggggcag gccgtttttc cgggagtggc tacactccct
    28741 atgcttgcta cgttactgtt tctccactaa caagacgttt ccaaagcccc aaactacccg
    28801 caaaatagcc ctctggaaag aaaaaagtgc aaaaaaatag aagctacgct gtgcagctcc
    28861 cgctaaaagt aaaacagagg atgccagtta cacatcctcc gtttcattct tcttcggctt
    28921 ttcaattagt atggcgctgt caatcgcgcc ctcgatgatc ggcaggtatt cttcggggca
    28981 gagtttcagc ttgtggctga ccctttgccg cagttcgctt tcctcccgca tgatctccgg
    29041 gttaaagtag cgttccacag gaagcccgca ggctttaata agctgtatga caaccggcag
    29101 actgggaatg gccccgtcgt tttcgatgtt ggcaagatag cgccagtcaa tatttatttt
    29161 ctctgccatt gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca catccgcgcc
    29221 gaaagtttca aagccgggac aatcttcaat attcgccata tcgcacctcc cacttacatt
    29281 gtatatttca tactctgccc gtggaatgtt ggtatatgca cctttcttaa agtttatatt
    29341 tcacattttg tttcttttat tcttcgagca aaagaattat aatgtaccct gtggaggtgt
    29401 actatggact acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg
    29461 ataaactact attgtgctgc gggacgcatc cccggcgctg taaagatggc gacgatctgg
    29521 ctcattccca aaactgcgga aaagccgatt gacaggcgta caaagaaagg aaaggcggtt
    29581 actgaatagt ataatgaaac cggcgcattt tggatatggt tttgaagaga gagaaagcat
    29641 ggtattttgt cacggaaaaa ctataagatg atagatggca ggctgctcca gaccgataaa
    29701 aagtattctc atctgaaaca gaaagaaaag atttacacat ggatgtatga agaagcgaaa
    29761 ctttatcatg acagaaatgg aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc
    29821 agccgtatat ggtagaattg aaaaggccgg catctggatt ctttatggtg aagtctccag
    29881 acattggata actgctggtc taatgcgcct ttgagtatgg ggtgattgat tttaataccg
    29941 gggaggtacg gctttttgag gaaaatattc tgggatttac atttggcgat tattacattt
    30001 cctgtaactg caaggacacg gatgatgaag atatgaggaa tatagacttg ctgattttct
    30061 actgtcttga acggtattag gactgaatct gctgcgcgga gtgcggcaag agcgaccgga
    30121 gggagtacac acaggccctg cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa
    30181 agaggccgcg cattttgctg aggcttgggg tgtccccaac aagcaaaacg agaacgccgc
    30241 aggcgggcaa gtggtggatc agaatatccg taaaggatat tctgatccat tgcttgccag
    30301 tattatcggg atgttttgac aaccgctgta atcgaaacat cccaaacatt aagaaggtgt
    30361 taattattat tatcttgatt taattcgttt aaaccaataa tatagataca tacaccatga
    30421 aaagaagcga gtatcccaat gacggatgat taaaatccga aaagaaatcg ctttgaaact
    30481 acagggaaac tacagactgt tatgttatct tcttaaaatg gagggatttt tatgtcgata
    30541 cgaattctac ttgtcgagga tgatgatcat atctgtaata cagtaagggc gtttctggct
    30601 gaggcaggat atcaggtgga tgcctgcaca gatggaaatg aggcatacac caagttttac
    30661 gaaaacactt atcaactggt tattcttgat attatgctgc ccggtatgaa cgggcatgaa
    30721 cttttgcgtg aatttcgtgc gaaaaatgat actcccattc tgatgatgac agccctgtcg
    30781 gatgacgaaa accaaatccg ggcgtttgat gcagaggcag acgactatgt aacaaagcca
    30841 ttcaagatgc agattttact aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg
    30901 gcaaaggaaa tccgtgtcgg caggctgaca cttctgccgg aggatttcac ggtactttgt
    30961 gacggtacag agctgcccct gacacgaaaa gaatttgaaa tccttttgct gctggtgcag
    31021 aacaaaggca gaaccttaac gcatgaaatc attttgtcac gtatatgggg atatgacttt
    31081 gagggtgatg gcagcacagt ccacactcat atcaaaaatt tgcgggccaa gctgccggaa
    31141 aatatcatca aaaccattcg cggtgtagga taccgattgg aggaatcata ataatggaaa
    31201 gaaaagggat tttcattaag gttttttcct atacgatcat tgtcctgcta ctggttgtcg
    31261 gtgtaacagc aacactgttt gcacagcaat ttgtgtctta tttcagagtg atggaattac
    31321 agcaaacagt aaaatcctat cagccattgg tggaactgat tcagaatagc gataggcttg
    31381 atattcaaga ggtggcaggg ctgtttcact acaataacca atcctttgag ttttatattg
    31441 aagataaaga gggaagcgta ctctatgcca caccaaatgc caatacatca aatagtttta
    31501 gacccgactt tctttatgtg gtacatagag atgataatat ttcgattgtt gctcaaaaca
    31561 aggcaggtgt gggattgctt tatcaagggc tgacaattcg gggaattgtt atgattgcga
    31621 taatggttgt attcagcctt ttatgcgcgt atatctttgc gcggcaaatg acaacgccga
    31681 tcaaagcctt agcggacagt gcgaataaaa tggcaaacct gaaagatgta ccgccgccgc
    31741 tggagcgaaa ggatgagctt ggcgcactgg ctcatgatat gcattccatg tatgtcaggc
    31801 tgaaagaaac catcgcaagg ctggaggatg aaatcgcaag ggaacatgag ttggaggaaa
    31861 cacagcgata tttctttgcg gcagcttctc atgagctaaa aacgcccatc gcggctacaa
    31921 gcgttctgtt ggagggaatg cttggaaata tcggtgacta caaagatcat tctaagtatc
    31981 tgcgcgaatg catcaaaatg atggataggc agggcaaaat catttccgaa atactggagc
    32041 ttgtcagcct gaatgatggg agaatcgtac ccatagctga accgttggac atagggcgca
    32101 cggttgccga gttgctgccc gattttcaaa ccttggcaga ggcaaacaac cagcggttcg
    32161 tcacagatat tccagccggg caaattgtcc tgtccgatcc gaggctgctc caaaaggcac
    32221 tatccaatgt catattgaat gcagttcaga acacgccgca gggaggcgag gtacggatat
    32281 ggagtgagcc tggtgctgaa aaatgccgcc tttttgtttt gaacatgggc gttcacattg
    32341 atgatactgc gcttccaagg ctgttcaccc cattctatcg cattgatcag gcgcgaagca
    32401 gaaaaagtgg gcgaagcggt ttaggacttg ccatcgtaca aaaaacgctg gatgccatga
    32461 gcctccagta tgcgctggaa aacacctcgg atggcgtttt gttctggctg gatttaccgc
    32521 tcacatcaac attgtaaata tttaaaactt aaatgatttt gaccgacagg tataaccctg
    32581 ccggtctttt tttgctgcta caggaaaact acagactggc tacagggaaa gtacagatgc
    32641 gcttgccata ataacaatcg taccagccac aaatcgtagt tttattgcaa aggaggcatt
    32701 cgatcaaatg gaaaaaagca actatcattc caatgcggat catcacaaac ggcatatgaa
    32761 acaatccgtg gaaaagcggg cttttctatg tgcgttcatt atttcgttca cagtctgcac
    32821 gctgtttttg aggtggagac tggcttccgt attggaggca acacagatac cgcccatccc
    32881 tgcaactcat acaggcagca gcactgacgt agtggagaat ttggaggaaa acgctcttgc
    32941 caccgccaaa gaacagggag atgaacagga atggagcctg attttagtga acaggcagaa
    33001 ccccatcccc gcacagtacg atgtggagct tgagcaacta tcaaatggtg agcggataga
    33061 tattcggatt tctccctatc ttcaagattt gtttgatgcc gcaagaactg atggagttta
    33121 cccgattgtc gcatccggat accgaacaac agaaaaacag caagaaatta tggatgaaaa
    33181 aattgccgaa tataaggcga aaggctacac ctctgcacag gctaaagcgg aagcagaaac
    33241 ttgggtggcc gtgccgggaa cgagcgagca tcagcttggt cttgctgtgg atatcaatgc
    33301 ggacggaatt cattcaacag gcaacgaggt ttatagatgg ctggatgaaa acagctatcg
    33361 ttttggtttt attcgtcgct acccaccaga caagacagag ataaccggtg tgagcaacga
    33421 gccgtggcat taccgatatg ttggcatcga agctgccaca gagatgtaca accaaggggt
    33481 ttgccttgag gaatatttaa aaccagaaaa atgagaaaag gatgttatgt tatggacaga
    33541 aaaagattga cacagcgttt cccgttcctg cttccaatga gacgggcgca gagaaaaatg
    33601 tgcttttatg cgggaatgag atttgacggc tgtcgctatg cgcagacgat aggggaaaaa
    33661 tcgctttccc atttgctctt tgaaacggat tgcgcattat acaaccacaa taccggattt
    33721 gacatgatat accaggaaaa caaggtgttc aacttaaagc tggcagcaaa gaccttaaac
    33781 ggcttattga taagaccggg ggaaaccttt tctttctggt ggctggtacg ccatgcggac
    33841 aaagataccc cctataaaga cggccttacg gtgaccaatg gtaaactcac caccatgtcg
    33901 ggcggcggta tgtgccagat gagcaattta ctattttgga tgttcctgca tacgccattg
    33961 acaattatcc agcgcagagg tcacgaagta aaggagtttc cagagccaaa cagtgacgag
    34021 attaaagggg tggatgcaac cattccagag ggctggattg atttaaaagt gcgaaacgat
    34081 accgactgca cctaccaaat atgggtgacc ttagatgatg agaaaatcat cggtcaggtg
    34141 tccgccgaca aagagcccca agcattatac aaaattacaa atggcagtat ccagtatgtc
    34201 cgtgaaagtg gcgggattta tgaatatgcc caggttaaac ggatgcaagt tgccttaggt
    34261 accggggaaa taatagattg caagctgctt tatacaaaca aatgcaaaat ctgttatccc
    34321 ctcccggaaa gtgtggatat tcaggaggag aaccaatgag aaaaagtgtg ggcattactg
    34381 tttttggatg cgaacaggat gaggcaaatg ctttccgcgc tttatcgccg gattttcata
    34441 ttatccctac gctgattagc gatgcgatat cggcagacaa cgcaaaattg gccgctggca
    34501 atcaatgcgt tagcgtaggc cataagtccg aggtttccga ggcgacaatt cttgcgctga
    34561 gaaaggtcgg ggtaaaatac atttctaccc gcagcatcgg ctgcgatcac attgatacga
    34621 ctgccgccga gagaatggga atctcggttg gcacggttgc gtattcgccg gacagcgttg
    34681 cggattatgc tttgatgctg atgctgatgg ccatacgggg tgcaaaaccc accatgcacg
    34741 ccgtggcgca acaagatttc agattggatc gtatccgggg gaaagaactg ggggatatga
    34801 ctgtgggagt tattggaacc ggccatatcg ggcaagcggt cgtcaaaagg ctgcggggat
    34861 ttggatgcca tgtgctggcc tatgataaca gccgaaaaat ggatgcagat tatgtccagc
    34921 ttgatgagct tctaaaaaac agcgatattg ttacgctcca tgtgccgctt tgtgcggata
    34981 cccgccatct gatcggtcag aagcaaattg gagagatgaa gcaaggcgca tttttaatca
    35041 acaccgcgcg cggagcgctt gtcgataccg gggcgctggt ggaggcgttg gaaagcggaa
    35101 agctgggtgg tgcggcactg gatgtgttgg agggcgagga tcagtttgtt tataccgatt
    35161 gctcgcagaa agtgcttgac catccctttt tgtcgcagct tttaaggatg ccgaatgtga
    35221 tcatcacacc ccatacggcg tactacactg agcgtgtgct gcaggatact acagaaaaaa
    35281 caatcaggaa ttgtctcaat tttgaaagga gtttacagca tgaatagaat aaaagtcgca
    35341 atcatcttcg gcggttgctc ggaggaacat gatgtgtcgg taaaatccgc aatagaaatt
    35401 gctgcgaaca ttgatacgga aaaattcgat ccgcactaca tcggaattac aaaaaacggt
    35461 gtatggaagc tatgcaagaa gccatgtacg gaatgggaag ccgacagtct ccccgccata
    35521 ctctccccgg ataggaaaac gcatgggctg cttgtcatga aagaaagcga atacgaaaca
    35581 cggcgtattg atgtggcttt cccggttttg catggcaaat tcggggagga tggtgcgata
    35641 caggggctgt ttgtattgtc tggtatcccc tatgtgggct gtgatattca aagctccgca
    35701 gcttgcatgg acaaatcact ggcctacatt cttacaaaaa atgcgggcat cgccgttccc
    35761 gaatttcaaa ttattgataa aggtgacaag ccggaggcgg gtgcgcttac ctaccctgtc
    35821 tttgtgaagc cggcacggtc aggttcgtcc tttggcgtaa ccaaagtaaa cggtacggaa
    35881 gaacttaacg ctgcgataga agcggcagga caatatgatg gaaaaatctt aattgagcaa
    35941 gcgatttcgg gctgtgaggt cgggtgtgcg gtcatgggga acgaggatga tttgattgtc
    36001 ggcgaagtgg atcaaatccg gctgagccac ggtatcttcc gcatccatca ggaaaacgag
    36061 ccggaaaaag gctcagaaaa tgcgatgatt acagttcccg cagacattcc ggtcgaggaa
    36121 cgaaatcggg tgcaggaaac ggcaaagaaa gtatatcggg tgcttggatg cagagggctt
    36181 gcccgtgttg atcttttttt gcaggaggat ggcggcatcg ttctaaatga ggtcaatacc
    36241 atgcccggtt ttacatcgta cagccgctac ccacgtatgg tggccgccgc aggaatcacg
    36301 cttcctgcac tgattgacag cctgattaca ttggcgttaa agaggtgata agtatggaaa
    36361 atggtttttt gtttttagat gaaatgttgc atggcgtccg ttgggatgcc aagtacgcca
    36421 catgggataa cttcacagga aaaccggtgg atgggtatga ggtgaatcgc atcatcggca
    36481 caaaggccgt ggcgtttgct ctgcgcgagg cacaaatcca tgcggctgcg cttggctatg
    36541 gcttgctttt atgggatgga tatcggccaa gaactgcggt ggactgcttc ctgcgttggg
    36601 cagcgcaacc ggaggacaat ctcacaaaag aaaaatttta ccccaatata gagcgagccg
    36661 agttgattac aaagggttat gtggcctcac aatccagcca tagccgtgga agcgcaattg
    36721 atcttacgct ctaccacctg gatacagggg aacttgtttc aatgggaagt aacttcgatt
    36781 ttatggacga acggtcgcat catacagcaa aagggatagg ggatgcagag gcacaaaatc
    36841 gaagatgctt gcgtaaaatc atggaaagca gcggatttca gtcttatcgc tttgaatggt
    36901 ggcactataa gttgattgat gagccatacc ccgataccta ttttaatttt gccgtttcat
    36961 aatgaaagta ttggttttgt atcagatttt ggatttgata aaatctatgc tggtcatgga
    37021 caatctttta ctggaaagtg gttaaagcat cttttgtaat acattgcaaa ttatagtatt
    37081 caatctgccc agcggcagaa aagaaaaaaa ccgctgttgg gcagaccttt cccacgccca
    37141 ttttgtattt tgctggcggc ggttttgaga aatcaaggcc gccgttcttt tctggcagca
    37201 aaaggtgtgg cggcccatag gaggatgccg ctatgctgtt ttgctattgc catgctctaa
    37261 tccacttcca ccactcaaaa aagccttgcc cctccacagg gctattccgg ttatgagtat
    37321 gtactatacc atgtaaatca gcagcatagc aaagcatttt ctcgcccggt aggtttgcga
    37381 cctgccgggc gatttttgtc gtcttttgcg gcccggctgt ccccaaaaaa attttttgag
    37441 aaattttcaa aaaggaggta tttagcgggt agctggcggc cttgtcaaag tcttgttagc
    37501 ggaaagggag agaaccacct aagtccccca cagaaagggg gtgagaagat ggatgctatc
    37561 ccccgcgact atgaagcgcg gtgcatgttc gatgcttttt gcaagacggt actgcgtaac
    37621 gaggccaaaa gctacttggc tgaaatgaag cgccgccgtg accgtgaagt gtcactaagc
    37681 tctttgtcac aggcagattt ggacaagctc tgtacagtag atcactaccc cagcgacagc
    37741 tttacgtttt cgtcgcatgg ctatgaccta catatcaaca atgagcttgt tgccgaagcc
    37801 tttgccgcgc tgcccagcat ggagcagagt attttaatcc tgcactgtgt tctggatatg
    37861 gcagatggtg agatcggcgg ccttgtggga atgtcccgga gtgccgttca gcggcacagg
    37921 acaaatacct tatcggagct gcggaaacag ctaaaggcgt tgatgccgaa gggaggttag
    37981 acgcggatga agaacccgaa ctacaagggc attgagcttt tgcccctttc ggtgatcgag
    38041 gccgcccgcg ctggggatgc cggagccgta gagcgcgttt tgcagtatta cagcggttac
    38101 atgaacaagc tgtgtacccg gacgctctat gacgaaaacg gctatccgca tgtgtgtctg
    38161 gacgaggcca tgaagcgccg tctggaaatc aagctcatcc acgctatcgt cacgaactga
    38221 cgaaccggcc ccggcagcat gaaacggctt accctttccc cgtttcggtg ttttcccggt
    38281 ggcctgatcg caccttgata aatatgcccg caagataacg gcggcgcatc atagggcata
    38341 cggacacatt ctgtctgtgc cgagccggac ggctggtgcg ccatgatact tttccctacg
    38401 ggaaaagcgg agcgaggaac accacgccgc aatgtgagcg tggcggcttg cgggcgacga
    38461 cgtacaggca ggactatgat actcccttat agccacagtc cgagcgttaa aagcgtcgca
    38521 ggcaatgggt agggccgcgt gagaaccacg cgggggtgaa agtcccgtgg agctgtgcca
    38581 gcagccgtcc gtctatgccc ctaaatcaca caaacctttg aatttgtgaa agggggctgc
    38641 attatgaacc atgatgtgcc tatttgggaa aaatacacgc tgacgattga ggaagcgtct
    38701 aaatatttcc gtatcggaga aaacaaattg cgtaagctgg ccgaggaaaa tccggcctct
    38761 ggctgggtca ttatgaatgg caaccgcatc cagatcaaac gcaagcagtt tgagaaaata
    38821 attgacactt tggacacaat ctaatagtga aaaagggccg cagatatgtt ataataattt
    38881 ataagcatat atgcggctct ttccgtcatg gaaaggagca tgacgagatg tcagaaaaaa
    38941 gacgagacag caaaaatcgc gttttgcggt ctggagagag ccagagaaaa gacggacgat
    39001 atgcttacaa atatgttgat acctttggaa aaccgcagtt tgtttactca tggaagttag
    39061 tgcctacgga taagaccccg gcaggtaaac gggaggatat ttcactccgg gaaaaggaga
    39121 aagaaatcca gaaagacctt gacgacggga tagacaccat cggcaagaaa atgaccgtct
    39181 gccagctcta cgccaagcag atcaggcacc ggggcaatgt gaagcacaac accaaactgg
    39241 gccgggaacg gctgatgcgt attctggaac aagatcggct ggggagctgc ccgattgaca
    39301 gtgtgaagct gtccgacgca aaggaatggg cgcttcggat gaaagaaaag ggcctttctt
    39361 ataagaccat caataacgac aagcgttccc tgaaagcggc tttttacacc gccatacagg
    39421 acgattgcat ccgaaaaaac ccgtttgact tccagcttag tgacgttctg gacgacgata
    39481 cggaaccaaa ggtgcctctt acaccagcac aggaagaaag ttttctgtcc tttattcaag
    39541 gtgataaggt ttaccaaaag cattatgacg caatcgttat tctgctgggg acagggcttc
    39601 gcatttcgga actgtgcgga ctgacggaca aagatttgga ttttgaaaac cgggtaatca
    39661 tcgtgagcca ccagcttttg agaaataccg gcgtcggtta ctatattgac gaacccaaga
    39721 cccaaagcgg cgtcagaaaa atccccatga atgaggaagt gtatcaggcg ttccagcgag
    39781 tgataaaaaa ccgcaaaggt gcaaagccgt tcattattga cgggtatgcc aatttcctgt
    39841 ttctcaaaca gaacggctat cccatgacgg cggtagatta tggcgggatg tttggcaggc
    39901 ttgtgaagaa gtacaacaag agccatgaag aagcgttgcc gaaaacgacg acgccccatg
    39961 ctatgcgtca tacattctgc acccggttag ccaatgcggg aatgaacccc aaagccttgc
    40021 agtacattat gggacattcc aatatcacca tgacgctgaa ctattatgca cacgccacat
    40081 tcgattccgc aagggcagaa atggaacggc tggcagcctg acacaaggcc ggttttacta
    40141 ctccatttac tacttttgag ggggaaatgg tgagaggtaa taagaacatt tgtaagtatc
    40201 taacaatatc aaaaatgccg gaaaagcccg gaaacacggg ctataccgac attcaagaac
    40261 ttctaaaaag ataatctaaa atcccatata atttttcctc aaaagctgat acaaagactt
    40321 atattgagcc tggagtagta aaaaagagaa atattatcgg tagtcccatc tttcagattc
    40381 catatattgg actagtcctt tcaaaaacgg catttgcatg gattcagggg tt
//
LOCUS       DAHHPA010000307         9935 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.guided.097, whole genome shotgun sequence.
ACCESSION   DAHHPA010000307 DAHHPA010000000
VERSION     DAHHPA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.guided.097"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(95..967)
                     /locus_tag="NBH55_003062"
     CDS             complement(95..967)
                     /locus_tag="NBH55_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1510961.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1095..1664
                     /locus_tag="NBH55_003063"
     CDS             1095..1664
                     /locus_tag="NBH55_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCD1510962.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1731..2468
                     /locus_tag="NBH55_003064"
     CDS             1731..2468
                     /locus_tag="NBH55_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1510963.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2699..3967
                     /locus_tag="NBH55_003065"
     CDS             2699..3967
                     /locus_tag="NBH55_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCD1510964.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4454..4918
                     /locus_tag="NBH55_003066"
     CDS             4454..4918
                     /locus_tag="NBH55_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1510965.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4902..5531
                     /locus_tag="NBH55_003067"
     CDS             4902..5531
                     /locus_tag="NBH55_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCD1510966.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5885..7363
                     /gene="msr(C)"
                     /locus_tag="NBH55_003068"
     CDS             5885..7363
                     /gene="msr(C)"
                     /locus_tag="NBH55_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCD1510967.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7410..7856
                     /locus_tag="NBH55_003069"
     CDS             7410..7856
                     /locus_tag="NBH55_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCD1510968.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8055..8570)
                     /locus_tag="NBH55_003070"
     CDS             complement(8055..8570)
                     /locus_tag="NBH55_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCD1510969.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8687..9889
                     /locus_tag="NBH55_003071"
     CDS             8687..9889
                     /locus_tag="NBH55_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1510970.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3166 a         1746 c         2066 g         2957 t
ORIGIN      
        1 gaacaaatta agaaacttat gttatttaat cattggatct tttgcttaac ttattcgttg
       61 ttaaaaagct gaaagcgaag gaaaaaaact ctacttaact gtatttctcc gttaggacat
      121 ctaaaaattt acgagcagca ggagaaagag gctggtcttt tttccaaatg agattcagtg
      181 aagcttgtaa tggcggatcg agtgggatga atactttttc cgtcccataa gtattgatga
      241 tcccatctag agctaatgca ccaaaagtcc cgttatcgac catcaaagaa gcattgtaca
      301 ataagttata tgttccgacg atcgataact gatctatgct gaaacctagc cattcagcta
      361 gttgactgtc cactgacgtt tgccgggaaa tgatcaaagg aacagttttc agttcctgcg
      421 gatggatgac tgtttgcttt gctaaaggat gattccgatg tatcaaaagt ccccaacgat
      481 cttgggctgg cagtcgaaat gactggtatt tttgtttgtt tgtcggttcg attacgagtc
      541 caaaatcaag taatccgcta tccagtttgt ctatgatatc atctgcattc ccgctatata
      601 aatgaaactg gatatcagga tgcgccttga tcaacgaatg aaggctatca gaaatgaaag
      661 agattgcttt agtttcgcct ccgccaatgt agatttctcc agagatgatt gcgtcatttg
      721 ttaaattgtt cgccgttttt tctaccaacg acaagatttc ttttcctttt gaataaagat
      781 atcgaccaga atccgtcaac tgtatctctc gggagcctcg agtgaaaagt gtcacaccaa
      841 gttcttcttc taaatctttc agttgttttg agagagtagg ttgcgtgata tgcagagatt
      901 ttgctgctcg gctgatattt ttttctctag ctactgctaa aaaatacgtc agtatacgaa
      961 tatccatttt ttccctcctg tttttaaaaa agcataccac taagtatagt taaaaggaat
     1021 agttaagtat gttttataag tattagacat cataagccgt ccgttataaa gtaaggataa
     1081 aaggagggag caaaatgaca agcctcatcg aaaaacaaat cgtcaataaa gagattcgag
     1141 taacggatcc gctctttgaa gtgatccatc agatccaagc ggaaaacgaa gagcctttag
     1201 cagcgctgaa tactgggtac catgaaccag cagatatccg caaacggcta gaaaatatca
     1261 ttagtgacaa agtagatgat acggtgactg ttctgctgcc tttttataca gatttcggca
     1321 aacatattag tatagggaaa aatgtcttta tcaatcgtca agtgatgttc gttgacttag
     1381 gtggcatctg tttagaagat tcagtattga ttggtcctcg tgtcaacctc atcacggtga
     1441 accatctaac cgatccaaaa gaacgaagag gcttgtcagt aaagccaatc cacatcaaga
     1501 aaaatgcttg gatcggagca ggagcaacta ttctgccagg cgtaacgatt ggcgagaatg
     1561 ccattgttgc agcgaatgca actgttacga aagatgtccc agacaatacc atcgttgcag
     1621 gaatccctgc caaaatcgtc aaacctgtcg aacggattat ttgaacagta agatgaaata
     1681 agagatagtg agtataagca agagattttt caaaacagga ggagaataga atgaatgatt
     1741 tatcagaaaa agtgattgtc atcatgggcg cttcaagcgg aatcggtgaa gcaacaacga
     1801 agctattagc agaaaaaggg gctaaactag ttatagctgc acgccgagaa gatcgcctaa
     1861 aagcaataaa agaatcttta ccagaagctg agctatatat tcaaacagca gatgtaagag
     1921 attttgcaca agtccaagca gttatcgatt tagcgatgga aaagttcggt cgaatcgatg
     1981 tattgtacaa taatgcagga atcatgccaa ccgctccatt agttgaaggt caccgtgatg
     2041 aatggcaaaa tatgcttgat atcaacatca tgggtgtgtt aaatggaatt tccgccgtac
     2101 ttccaatcat ggaaaaacaa aaatctgggc atatcatttc aactgattct gtggcgggac
     2161 atgttgttta tcctgattcc gccgtttatt gcggaacaaa atttgctgtc cgtgcaatca
     2221 tggaaggatt acgtcaagag caacggcaaa ataacatcaa atctacgatc atctctccag
     2281 gagctgttca aactgaatta tatcaaacca tcagcgacaa aaaggctgct cttgaacttc
     2341 atgaagcgca aaaagaatgg ggattaacat ctgaagatat agcttctgct gtagcatttg
     2401 cgatagaaac gccagaccgg atgtctgtca gcaacatgat tatccgtccg acgacacagg
     2461 aagtataatc cataacggat aatatccaga gagaattact tgttaaatag ttattcaaac
     2521 gaccatcaat ttaaggcgtg aatggagaag aatattcttc atttacgtct ttttatgcag
     2581 aaaaagcaaa tttattaaaa agaaacaaat gttatttaaa tatggacatt gacttgcttt
     2641 tactttttta gttacactgt tatttaggaa aagtttcata attcaaagga gaaaaaagat
     2701 gaaagcaaag aatttattat ttagcgttat tcttttagga aatctagcag ttgccaacac
     2761 aacagtgcta gctgaagaat cgacagaaac tgtcgcaact cagacatcga gttcttcaga
     2821 aacaggaatt gaaaacgata cagaaagtac tttgcagtca gctactgtct cagaaaatac
     2881 aatttcaacc gaagaaccaa caactgaaac aacagaacaa acacaaaccg aagacagtac
     2941 atcagcagaa gctcagaaca atccagagaa tgcagaagaa atcacgaacc aattgacaga
     3001 tgggcaaaca ttgacacata cccatcaagc agatttgtct gaattcgaaa actatactgg
     3061 ctatgctttt tatgctcgct catctgctaa ttctcaacaa gcgttcattg atagtattgc
     3121 ctcaactgca caaagtttgg caagtgccaa tgacttatat gcatcagtca tgattgctca
     3181 agccatcgtt gaaagtggct gggggaacag tactttggct tctgccccta attacaatct
     3241 atttgggatt aaaggaagct ataatggcca atctgttacg atgcctactt cggaatatgt
     3301 gaatgggcaa tggattacgg tgaatgccgc ttttagaaaa tatccttcgt acaaagaatc
     3361 attgcaagat aacgtaacgg tattgaagac gacttctttt cagccaggcg tttactacta
     3421 ttccggtgca tggaaaagca atacgaactc ttacaaagat gctacggctt ggctaacagg
     3481 tcgttatgcc actgcgccta attatggttc tacattgaac aatgtaatcg aaacctataa
     3541 tctgacacag tatgatacag caccaacttc aacttctccg atgtatcgtc tgtataaccg
     3601 tcatacagga gaacatttat atacattgaa tgccggagaa aaaaattatc tgccaactgt
     3661 aggctgggaa tatgaaggga ttgcttggca agcaccaaac agcggacaac ctgtctaccg
     3721 tttatacaat ccttacagtg gagaccatca ttacacaatg gcacaatctg agatcaactc
     3781 tttaacaaaa atcggttggc gttatgaagg tctaagtttt tattctggtg gttccaagcc
     3841 aatttatcga ctatttaatc caaatgaaaa aactggtacc catcactata ccttgagtgc
     3901 taaggaaaga gattggctaa caccgatggg ctggagatat gaaggaatag gattttatgg
     3961 ttattaaaaa gaggtagtga aaaagctgcc ctgcatcaag tagtaagaac agccaaacaa
     4021 aaacagattt tcatgttttt tgtttggttg aacttattac cgcagatgca agtttttgaa
     4081 caccgtttac tcaaaaaagg ttgtgacaac atcttgtcat aactttttta ttactgttta
     4141 agcatactga tccaatttct aatattcgac gggattcttg caaaaggcga tttcggaaat
     4201 ttttggcggt gtaaaattat ctactgcaga agagctatct tctatagttt gataagaatc
     4261 aataatcttg gttttagcgt tgtgctttct ttatcatttt ttccgttcat tctacaaaaa
     4321 atttcttttc tctatacttt tctattgaga gcgttttttt tattacacag ctgattcttt
     4381 gacttttttc tttttttatt ctattattga caagtcaatc gttgacgagt caattattag
     4441 aaaggaggaa attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc
     4501 cgatcaaaaa atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga
     4561 gatcatgatg agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt
     4621 gcagattgat caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt
     4681 ttcgcgtcag agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc
     4741 ccagaagaaa atcaagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc
     4801 ggctttcagc gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca
     4861 actttgcaat ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt
     4921 tttcaacaat tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc
     4981 cacacgaaga aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata
     5041 tgaaaccatg gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt
     5101 tgatccgttt caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg
     5161 atatgcaatg ttacgaatat ggcgaagaaa tctacaacca agcttacgaa tcagggaaga
     5221 tgccaaaaga agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt
     5281 caagagaaga aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca
     5341 tgttagttgc gcgtgatcat ggctatgaaa caaatccaat tggtggattc gaagcagatc
     5401 agttggcaga ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa
     5461 taggaaaagc ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta
     5521 tgttcgaata aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg
     5581 tggtaaccta ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga
     5641 acttttgaat aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg
     5701 gagtatgtcc attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga
     5761 tttagtcata gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca
     5821 gatttttctg ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa
     5881 acatatggaa aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct
     5941 agaactatct attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga
     6001 aaatggtgtc ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca
     6061 tgggaaaatc caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga
     6121 agcaaaggac ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc
     6181 agagcggaaa tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc
     6241 ttatgaacaa gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga
     6301 tcgactgatt gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg
     6361 ctattttcta gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga
     6421 gttttcgggg aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct
     6481 acgagaagca gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa
     6541 gaaaaaacaa gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag
     6601 actggatcgg ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa
     6661 tgcgaaaaca ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat
     6721 gaaacaaatc cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat
     6781 gggacaaaat gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca
     6841 gttttcttta ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt
     6901 attagaacat atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca
     6961 agtatatcag caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat
     7021 gagacaaaca gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc
     7081 tcaggaaact ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac
     7141 gattgctttg ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt
     7201 tattgatatg gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat
     7261 tttgtttact tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat
     7321 aaaagggcag aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt
     7381 tcgtgacaaa tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt
     7441 ttacaacaat gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc
     7501 atcaagatct tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt
     7561 tcattttgga tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag
     7621 taaatccaga aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa
     7681 tggtgacatg tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat
     7741 tctctgctta tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga
     7801 tcatcgttgg cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga
     7861 acactgttat ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt
     7921 ctagaaaaat agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt
     7981 tccaactgta ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag
     8041 gttctgttta tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat
     8101 tggagttcct gctgacaaag cataattctg cgctggatca caacataacg tccaaattct
     8161 tgtttcttgt ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc
     8221 agctgttgtt ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa
     8281 gcatagattt ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc
     8341 cattcctcta caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc
     8401 tcgtctttgt ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag
     8461 atctgactgt gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa
     8521 cgtggtttgg ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta
     8581 cctctcgtct tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata
     8641 ttgacatgta aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga
     8701 gaatcaaata gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct
     8761 agttgtagca gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt
     8821 aaaaacgatt gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag
     8881 ttacatgtta gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg
     8941 tttgaaaaaa acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg
     9001 attatctggg aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc
     9061 tttatttgtc tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa
     9121 agcaatcatg atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg
     9181 tggggtcttg ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt
     9241 ttgcggtttt atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt
     9301 aggcctttgg gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat
     9361 cgcattgtta tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat
     9421 tggctattct gaattagaag tcggttttgt attttttggc tggggtgtgc ttctggctat
     9481 tgcttcgatt ttcgttgcac cggctcttga gcggaagttt acgacttatc ggacaatttt
     9541 agcagcattt gtcttattca ttatttgttt gctgttatta ggagctggaa cttctatgcc
     9601 agtccttgta ccgatcagca ttgtgttagc aggatttttc caaggaatta tcaatacact
     9661 gttgacgacg attgctatgg aaataccagg tctgcaacga aacgttgctt cgtccagcta
     9721 tagttttgtc cgctttttcg gtggagcttt agcaccattt attgcaggtg tgattggtga
     9781 acatattggt gaaagctatg cgttttattt tgcaggtgcc attgttctta tcagtgtatg
     9841 ttgtatcatt tatcaccgta attattttgc tacaaatgaa gaagaataag aaatcagagg
     9901 ttgtgaaaaa gctgtcctgc atcaagtcat aagaa
//
LOCUS       DAHHPA010000308         8285 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.guided.109, whole genome shotgun sequence.
ACCESSION   DAHHPA010000308 DAHHPA010000000
VERSION     DAHHPA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.guided.109"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            60..977
                     /locus_tag="NBH55_003072"
     CDS             60..977
                     /locus_tag="NBH55_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1510971.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            980..3157
                     /locus_tag="NBH55_003073"
     CDS             980..3157
                     /locus_tag="NBH55_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1510972.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3154..4155
                     /locus_tag="NBH55_003074"
     CDS             3154..4155
                     /locus_tag="NBH55_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCD1510973.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4152..5084
                     /locus_tag="NBH55_003075"
     CDS             4152..5084
                     /locus_tag="NBH55_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1510974.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5329..5445
                     /locus_tag="NBH55_003076"
     CDS             5329..5445
                     /locus_tag="NBH55_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCD1510975.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5461..7380
                     /gene="tet(M)"
                     /locus_tag="NBH55_003077"
     CDS             5461..7380
                     /gene="tet(M)"
                     /locus_tag="NBH55_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCD1510976.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7499..7666
                     /locus_tag="NBH55_003078"
     CDS             7499..7666
                     /locus_tag="NBH55_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCD1510977.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7726..8079)
                     /locus_tag="NBH55_003079"
     CDS             complement(7726..8079)
                     /locus_tag="NBH55_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510978.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
BASE COUNT         2692 a         1491 c         1697 g         2405 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcactctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgtg attatgaaaa atcccaaaga ttctgaatca ctggctattg
      181 atattctgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtacgagg gttgatgaaa gtgattgagg
      301 aattacgggt tgagaatacg ccactaagta ccagtatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcacat ttattattca gtaatggtta tgtggagcag tctatcagct
      421 tagaaaaaca actgaacatt atacaggttg cggacttggt acttcccgac aaggaaactt
      481 cctttgagga atataccact atggagcttt tatccgttgc tatgctgatt gtcattagta
      541 cctttgcttt agactttatc catacagacc gaagcatttt caagattgta gatttagacg
      601 aagcatggag ctttttacag gtagcacaag gaaaaacact atctatgaag ctggttcggg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagac gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcatt tcgttccact gaccttaacg
      781 agattaaaaa gaccttagcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatagtaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggcttc cgtggaactg gtcggacggg attggaaaac aggtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ttgtatgttt ccaatgcgac
     1321 aggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggtaagaat atgcagacct tagctggtgt gagtgcaaac ggattttcaa cagagggttt
     1441 ctatgttgga ttcctcttac tcttgatttt ggttcttggg gtttatgttg cctatacggg
     1501 actgataaag agagaaacca caaaggcaat tcatgccatt atgaattttg tgctggtgtt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagtaatg ccagtttatc acttggcacg aagattgtca tgccccattc
     1681 cgatagtcaa ggcaaggaca gcgtggactt aatcagagat agcctgtttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatacaa cagttcagac attgaaagta tcggtattga
     1801 ccgtgtggaa agcctgctct ccaccagccc agattccaac aatggcgaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagacagaag caataccaat ctaaccatta caaagaccat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttc aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtgtga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gttccatgac
     2401 tactctaggg gctgggtctg ccattgttac aggtaaaaaa ggacagtcgg gttcggggag
     2461 ttctgcaagg acacaagcag atcactcccg accagacgga aaggaaaaat caacacttgg
     2521 aaaacgtatc ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagacac
     2581 tgctagtggt ttgaaagaac aggttaaaga tttgccgacc aatgcaagat atgcagtata
     2641 tcaaggaaaa tccaaagtaa aagagaatgt ccgtgattta accagtagta tttctcaaac
     2701 caaagcggac agagccagtg gacgcaagga acagcaggaa caaaggcgaa aaaccattgc
     2761 gaagcgtcgc tctgaaatgg aacaggtcaa acagaaaaaa cagcctgctt cttctgttca
     2821 tgaaagaccg actacaagac aagaacaata tcatgatgaa cagacctcaa aacagtctaa
     2881 tattcagact tcatataagg aatctcaaca agccaaacaa gagcgtccag cagttaagtc
     2941 cgatttttca agtccaaaag tggaacgcca aggcaatacc gttcaagaaa aaaccgttca
     3001 aaagccagca acttcaacca ctacagcaga tagaacttca caacgtccaa tcacaaaaga
     3061 acgtccgtct actgttcaaa gagtaccact acaaaataca agaagtagac caccaatcaa
     3121 aaccgccacc attaagaaag tcggtaagaa accatgaagt tgaaaacttt agtgattggt
     3181 ggttctggat tattcttgat ggtcttctca ctgcttctgt ttgttgccat tttattttca
     3241 gatgaacagg acagcggaat ttccaatatt cattatggag gtgtgaatgt ttccgcagaa
     3301 gtgctggctc ataagcctat ggtagaaaaa tatgccaaag aatatggcgt tgaagaatat
     3361 gtcaacatac ttcttgcgat tatacaggtg gaatcgggcg gtactgcgga agatgttatg
     3421 cagtcctcgg aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag
     3481 caaggtgtga agtatttcag tgaattatta gccagtagcg aaaggctcag tgtagattta
     3541 gaatcggtta tccagtccta caattatggt ggtggtttct tagggtatgt ggctaatcgt
     3601 ggaaataaat atacctttga actggctcaa agtttctcaa aagagtattc aggtggcgaa
     3661 aaagtgtctt accccaatcc catagccata cctatcaatg ggggctggcg atacaactat
     3721 ggcaatatgt tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat
     3781 gatacggtac aagccatcat ggacgaagca ctgaaatatg agggctggcg atacgtttac
     3841 ggtggagctt ccccgactac ttcttttgat tgtagcggac tgacacaatg gacgtatgga
     3901 aaagctggaa ttaacttacc acgaaccgca caacagcaat atgatgtgac ccagcatatc
     3961 ccactatcgg aagcacaagc tggcgatttg gttttctttc attctaccta taacgctggc
     4021 tcttatatta ctcatgttgg gatatacctt ggcaataacc gtatgtttca tgcaggcgac
     4081 ccaatcggtt atgccgactt aacaagcccc tactggcaac agcatttagt gggagcagga
     4141 cgaatcaaac aatgagaaag gaagatttaa tgatgaaatt tagaaaaaat cagaataaag
     4201 aaaaacagat accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cataaaaagg
     4261 ttgtgattgc cttgtgggta cttttagggc ttagtttcag ctttgcgata ttcaagcact
     4321 ttacagctat agatactcat actattcacg aaacaactat catagaaaag gaatacgttg
     4381 atactcatca tgtagaaaat tttgtagaga actttgcgaa agtctactat tcatgggagc
     4441 aatccgataa gtccattgat aatcgaatgg aaagtctaaa aggctatctg acagatgaac
     4501 ttcaagctct caatgttgat acagtacgca aagatattcc tgtatcgtct tctgtaagag
     4561 gatttcagat atggacggta gagccaactg gcgacaatga gtttaatgta acctacagtg
     4621 tagaccagct cattacagag ggagaaaata caaagaccgt ccactctgct tatatagtga
     4681 gtgtctatgt agatggttct ggaaatatgg tactggttaa gaatccgacc attaccaaca
     4741 tacctaagaa atcaagttat aaaccaaaag ccattgaaag tgaggggacg gttgattcca
     4801 ttacaaccaa tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga
     4861 cagccagtga actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt
     4921 acatctttca agaactggta aatcctattc acaatcgtaa ggataatcaa gtcacggtat
     4981 cgctgacagt ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt
     5041 tggtacttga aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt
     5101 attacagcta ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa
     5161 atatgctctt acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc
     5221 aaggtattat taaataaact gtcaatttga tagcgggaac aaataattgg atgtcctttt
     5281 ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag
     5341 tatccggaga atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat
     5401 aagagtattt atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac
     5461 atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca
     5521 gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca
     5581 acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata
     5641 acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat
     5701 ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct
     5761 gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg
     5821 attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt
     5881 tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg
     5941 tatcctaatg tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag
     6001 ggaaacgatg accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc
     6061 gaacaagagg aaagcataag atttcagaat tgttctctgt tccctcttta tcatggaagt
     6121 gcaaaaagta atatagggat tgataacctt atagaagtta ttactaataa attttattca
     6181 tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga atatacaaaa
     6241 aaaagacaac gtcttgcata tatacgcctt tatagtggag tactacattt acgagattcg
     6301 gttagagtat cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc aataaatggt
     6361 gaattatgta agattgatag agcttattct ggagaaattg ttattttgca aaatgagttt
     6421 ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa
     6481 aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg
     6541 ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat
     6601 tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt
     6661 gcactgttgc aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat
     6721 atggagagac cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct
     6781 ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat
     6841 gagagctcgg tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg
     6901 atacgctatg gttgtgaaca aggattgtat ggttggaatg tgacggactg taaaatctgt
     6961 tttaagtatg gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct
     7021 cctattgtat tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt
     7081 agttttaaaa tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa
     7141 tattgtgcga acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa
     7201 atccctgctc ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt
     7261 agtgtttgtt taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag
     7321 ccccgtcgtc caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag
     7381 tgtattttat gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa
     7441 taaagatttg aattaaagtg taaaggagga gatagttatt ataaactaca agtggatatt
     7501 gtgtcctgta tgtggaaata aaacacgatt aaagataagg gaagatactg aattaaaaaa
     7561 attccccctc tattgtccga aatgcagaca agaaaattta attgaaataa agcagttcaa
     7621 agtaactgtg attacagagc cagacgcaaa gacgcagagc cgataaaatg agattaatac
     7681 aatctcattt tatcggctct ttccgttatg tatggattct tttaattagt cttcgatgtt
     7741 tcttgcttcg ttgataccgc tggctaaaga ttccattatg gatagttctt tgtctgtaaa
     7801 gctatccatg tatttctcta tctgtaatcg tcgggtgctt tttaccaagt tattagcagg
     7861 taagaaaaat tcatcaacgg aaacatgaag taacgataca aggtcataaa gaacttgtat
     7921 gctggggtgt tgccctttat tttcaatatt agttaagtac cgtgggtcaa tttcaatcaa
     7981 tgctcccact tgttcacgag ttaaacctcg tttcaatcga gcttctttaa tggctaaacc
     8041 aaaggctcta aaatcatatt tatcttcttt tttacgcata gtagaccacc tctatacatt
     8101 ttattgttcc tactgaatta aaaacaggta tagaaaaacg tgttatatgg tttataggtt
     8161 tatatttaat aaaaagcact actaaacgcc aataaaaaaa accgttatat ggtagtgcta
     8221 tttacgctgt taaaatattg tatattactt ccaaatggcg gtttgttgga ggtcaacgtc
     8281 gccat
//
LOCUS       DAHHPA010000309         8284 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.guided.110, whole genome shotgun sequence.
ACCESSION   DAHHPA010000309 DAHHPA010000000
VERSION     DAHHPA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.guided.110"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            60..977
                     /locus_tag="NBH55_003080"
     CDS             60..977
                     /locus_tag="NBH55_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1510979.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            980..3157
                     /locus_tag="NBH55_003081"
     CDS             980..3157
                     /locus_tag="NBH55_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1510980.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3154..4155
                     /locus_tag="NBH55_003082"
     CDS             3154..4155
                     /locus_tag="NBH55_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCD1510981.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4152..5084
                     /locus_tag="NBH55_003083"
     CDS             4152..5084
                     /locus_tag="NBH55_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1510982.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5329..5445
                     /locus_tag="NBH55_003084"
     CDS             5329..5445
                     /locus_tag="NBH55_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCD1510983.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5461..7379
                     /gene="tet(M)"
                     /locus_tag="NBH55_003085"
                     /pseudo
     CDS             5461..7379
                     /gene="tet(M)"
                     /locus_tag="NBH55_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
     gene            7498..7665
                     /locus_tag="NBH55_003086"
     CDS             7498..7665
                     /locus_tag="NBH55_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCD1510984.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7725..8078)
                     /locus_tag="NBH55_003087"
     CDS             complement(7725..8078)
                     /locus_tag="NBH55_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1510985.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
BASE COUNT         2691 a         1491 c         1697 g         2405 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcactctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgtg attatgaaaa atcccaaaga ttctgaatca ctggctattg
      181 atattctgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtacgagg gttgatgaaa gtgattgagg
      301 aattacgggt tgagaatacg ccactaagta ccagtatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcacat ttattattca gtaatggtta tgtggagcag tctatcagct
      421 tagaaaaaca actgaacatt atacaggttg cggacttggt acttcccgac aaggaaactt
      481 cctttgagga atataccact atggagcttt tatccgttgc tatgctgatt gtcattagta
      541 cctttgcttt agactttatc catacagacc gaagcatttt caagattgta gatttagacg
      601 aagcatggag ctttttacag gtagcacaag gaaaaacact atctatgaag ctggttcggg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagac gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcatt tcgttccact gaccttaacg
      781 agattaaaaa gaccttagcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatagtaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggcttc cgtggaactg gtcggacggg attggaaaac aggtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ttgtatgttt ccaatgcgac
     1321 aggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggtaagaat atgcagacct tagctggtgt gagtgcaaac ggattttcaa cagagggttt
     1441 ctatgttgga ttcctcttac tcttgatttt ggttcttggg gtttatgttg cctatacggg
     1501 actgataaag agagaaacca caaaggcaat tcatgccatt atgaattttg tgctggtgtt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagtaatg ccagtttatc acttggcacg aagattgtca tgccccattc
     1681 cgatagtcaa ggcaaggaca gcgtggactt aatcagagat agcctgtttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatacaa cagttcagac attgaaagta tcggtattga
     1801 ccgtgtggaa agcctgctct ccaccagccc agattccaac aatggcgaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagacagaag caataccaat ctaaccatta caaagaccat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttc aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtgtga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gttccatgac
     2401 tactctaggg gctgggtctg ccattgttac aggtaaaaaa ggacagtcgg gttcggggag
     2461 ttctgcaagg acacaagcag atcactcccg accagacgga aaggaaaaat caacacttgg
     2521 aaaacgtatc ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagacac
     2581 tgctagtggt ttgaaagaac aggttaaaga tttgccgacc aatgcaagat atgcagtata
     2641 tcaaggaaaa tccaaagtaa aagagaatgt ccgtgattta accagtagta tttctcaaac
     2701 caaagcggac agagccagtg gacgcaagga acagcaggaa caaaggcgaa aaaccattgc
     2761 gaagcgtcgc tctgaaatgg aacaggtcaa acagaaaaaa cagcctgctt cttctgttca
     2821 tgaaagaccg actacaagac aagaacaata tcatgatgaa cagacctcaa aacagtctaa
     2881 tattcagact tcatataagg aatctcaaca agccaaacaa gagcgtccag cagttaagtc
     2941 cgatttttca agtccaaaag tggaacgcca aggcaatacc gttcaagaaa aaaccgttca
     3001 aaagccagca acttcaacca ctacagcaga tagaacttca caacgtccaa tcacaaaaga
     3061 acgtccgtct actgttcaaa gagtaccact acaaaataca agaagtagac caccaatcaa
     3121 aaccgccacc attaagaaag tcggtaagaa accatgaagt tgaaaacttt agtgattggt
     3181 ggttctggat tattcttgat ggtcttctca ctgcttctgt ttgttgccat tttattttca
     3241 gatgaacagg acagcggaat ttccaatatt cattatggag gtgtgaatgt ttccgcagaa
     3301 gtgctggctc ataagcctat ggtagaaaaa tatgccaaag aatatggcgt tgaagaatat
     3361 gtcaacatac ttcttgcgat tatacaggtg gaatcgggcg gtactgcgga agatgttatg
     3421 cagtcctcgg aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag
     3481 caaggtgtga agtatttcag tgaattatta gccagtagcg aaaggctcag tgtagattta
     3541 gaatcggtta tccagtccta caattatggt ggtggtttct tagggtatgt ggctaatcgt
     3601 ggaaataaat atacctttga actggctcaa agtttctcaa aagagtattc aggtggcgaa
     3661 aaagtgtctt accccaatcc catagccata cctatcaatg ggggctggcg atacaactat
     3721 ggcaatatgt tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat
     3781 gatacggtac aagccatcat ggacgaagca ctgaaatatg agggctggcg atacgtttac
     3841 ggtggagctt ccccgactac ttcttttgat tgtagcggac tgacacaatg gacgtatgga
     3901 aaagctggaa ttaacttacc acgaaccgca caacagcaat atgatgtgac ccagcatatc
     3961 ccactatcgg aagcacaagc tggcgatttg gttttctttc attctaccta taacgctggc
     4021 tcttatatta ctcatgttgg gatatacctt ggcaataacc gtatgtttca tgcaggcgac
     4081 ccaatcggtt atgccgactt aacaagcccc tactggcaac agcatttagt gggagcagga
     4141 cgaatcaaac aatgagaaag gaagatttaa tgatgaaatt tagaaaaaat cagaataaag
     4201 aaaaacagat accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cataaaaagg
     4261 ttgtgattgc cttgtgggta cttttagggc ttagtttcag ctttgcgata ttcaagcact
     4321 ttacagctat agatactcat actattcacg aaacaactat catagaaaag gaatacgttg
     4381 atactcatca tgtagaaaat tttgtagaga actttgcgaa agtctactat tcatgggagc
     4441 aatccgataa gtccattgat aatcgaatgg aaagtctaaa aggctatctg acagatgaac
     4501 ttcaagctct caatgttgat acagtacgca aagatattcc tgtatcgtct tctgtaagag
     4561 gatttcagat atggacggta gagccaactg gcgacaatga gtttaatgta acctacagtg
     4621 tagaccagct cattacagag ggagaaaata caaagaccgt ccactctgct tatatagtga
     4681 gtgtctatgt agatggttct ggaaatatgg tactggttaa gaatccgacc attaccaaca
     4741 tacctaagaa atcaagttat aaaccaaaag ccattgaaag tgaggggacg gttgattcca
     4801 ttacaaccaa tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga
     4861 cagccagtga actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt
     4921 acatctttca agaactggta aatcctattc acaatcgtaa ggataatcaa gtcacggtat
     4981 cgctgacagt ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt
     5041 tggtacttga aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt
     5101 attacagcta ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa
     5161 atatgctctt acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc
     5221 aaggtattat taaataaact gtcaatttga tagcgggaac aaataattgg atgtcctttt
     5281 ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag
     5341 tatccggaga atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat
     5401 aagagtattt atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac
     5461 atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca
     5521 gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca
     5581 acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata
     5641 acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat
     5701 ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct
     5761 gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg
     5821 attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt
     5881 tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg
     5941 tatcctaatg tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag
     6001 ggaaacgatg accttttgag aaatatatgt ccggtaaatc attagaagca ttggaactcg
     6061 aacaagagga aagcataaga tttcagaatt gttctctgtt ccctctttat catggaagtg
     6121 caaaaagtaa tatagggatt gataacctta tagaagttat tactaataaa ttttattcat
     6181 caacacatcg aggtccgtct gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa
     6241 aaagacaacg tcttgcatat atacgccttt atagtggagt actacattta cgagattcgg
     6301 ttagagtatc agaaaaagaa aaaataaaag ttacagaaat gtatacttca ataaatggtg
     6361 aattatgtaa gattgataga gcttattctg gagaaattgt tattttgcaa aatgagtttt
     6421 tgaagttaaa tagtgttctt ggagatacaa aactattgcc acagagaaaa aagattgaaa
     6481 atccgcaccc tctactacaa acaactgttg aaccgagtaa acctgaacag agagaaatgt
     6541 tgcttgatgc ccttttggaa atctcagata gtgatccgct tctacgatat tacgtggatt
     6601 ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg
     6661 cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata
     6721 tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt
     6781 tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg
     6841 agagctcggt ttctcttgga tacttaaatc aatcatttca aaatgcagtt atggaaggga
     6901 tacgctatgg ttgtgaacaa ggattgtatg gttggaatgt gacggactgt aaaatctgtt
     6961 ttaagtatgg cttatactat agccctgtta gtaccccagc agattttcgg atgcttgctc
     7021 ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga attgttagag ccatatctta
     7081 gttttaaaat ttatgcgcca caggaatatc tttcacgagc atacaacgat gctcctaaat
     7141 attgtgcgaa catcgtagac actcaattga aaaataatga ggtcattctt agtggagaaa
     7201 tccctgctcg gtgtattcaa gaatatcgta gtgatttaac tttctttaca aatggacgta
     7261 gtgtttgttt aacagagtta aaagggtacc atgttactac cggtgaacct gtttgccagc
     7321 cccgtcgtcc aaatagtcgg atagataaag tacgatatat gttcaataaa ataacttagt
     7381 gtattttatg ttgttatata aatatggttt cttgttaaat aagatgaaat attttttaat
     7441 aaagatttga attaaagtgt aaaggaggag atagttatta taaactacaa gtggatattg
     7501 tgtcctgtat gtggaaataa aacacgatta aagataaggg aagatactga attaaaaaaa
     7561 ttccccctct attgtccgaa atgcagacaa gaaaatttaa ttgaaataaa gcagttcaaa
     7621 gtaactgtga ttacagagcc agacgcaaag acgcagagcc gataaaatga gattaataca
     7681 atctcatttt atcggctctt tccgttatgt atggattctt ttaattagtc ttcgatgttt
     7741 cttgcttcgt tgataccgct ggctaaagat tccattatgg atagttcttt gtctgtaaag
     7801 ctatccatgt atttctctat ctgtaatcgt cgggtgcttt ttaccaagtt attagcaggt
     7861 aagaaaaatt catcaacgga aacatgaagt aacgatacaa ggtcataaag aacttgtatg
     7921 ctggggtgtt gccctttatt ttcaatatta gttaagtacc gtgggtcaat ttcaatcaat
     7981 gctcccactt gttcacgagt taaacctcgt ttcaatcgag cttctttaat ggctaaacca
     8041 aaggctctaa aatcatattt atcttctttt ttacgcatag tagaccacct ctatacattt
     8101 tattgttcct actgaattaa aaacaggtat agaaaaacgt gttatatggt ttataggttt
     8161 atatttaata aaaagcacta ctaaacgcca ataaaaaaaa ccgttatatg gtagtgctat
     8221 ttacgctgtt aaaatattgt atattacttc caaatggcgg tttgttggag gtcaacgtcg
     8281 ccat
//
LOCUS       DAHHPA010000310         8048 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.guided.112, whole genome shotgun sequence.
ACCESSION   DAHHPA010000310 DAHHPA010000000
VERSION     DAHHPA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.guided.112"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            complement(134..577)
                     /locus_tag="NBH55_003088"
     CDS             complement(134..577)
                     /locus_tag="NBH55_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCD1510986.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="NBH55_003089"
     CDS             complement(795..2135)
                     /locus_tag="NBH55_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1510987.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="NBH55_003090"
     CDS             2404..3300
                     /locus_tag="NBH55_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCD1510988.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="NBH55_003091"
     CDS             complement(3450..4391)
                     /locus_tag="NBH55_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCD1510989.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="NBH55_003092"
     CDS             4517..5638
                     /locus_tag="NBH55_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD1510990.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="NBH55_003093"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="NBH55_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCD1510991.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="NBH55_003094"
     CDS             7426..7992
                     /locus_tag="NBH55_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD1510992.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2670 a         1503 c         1594 g         2281 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcag
//
LOCUS       DAHHPA010000311         5289 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.guided.139, whole genome shotgun sequence.
ACCESSION   DAHHPA010000311 DAHHPA010000000
VERSION     DAHHPA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.guided.139"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            3..1409
                     /locus_tag="NBH55_003095"
     CDS             3..1409
                     /locus_tag="NBH55_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510993.1"
                     /translation="MDFFIYTKYFILSSFYYGEKFFKKIDAAFLFSCLYTFATYRTVD
                     IYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSAL
                     IACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFK
                     TDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVS
                     TWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLII
                     GATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKE
                     RPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVV
                     NDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKL
                     KDSRYVTKKEINKKNENK"
     gene            2045..2542
                     /gene="dfrG"
                     /locus_tag="NBH55_003096"
     CDS             2045..2542
                     /gene="dfrG"
                     /locus_tag="NBH55_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCD1510994.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2614..4566)
                     /locus_tag="NBH55_003097"
     CDS             complement(2614..4566)
                     /locus_tag="NBH55_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCD1510995.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4573..4809)
                     /locus_tag="NBH55_003098"
     CDS             complement(4573..4809)
                     /locus_tag="NBH55_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1510996.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1690 a          864 c          877 g         1858 t
ORIGIN      
        1 gtatggattt tttcatttac acaaaatatt ttatactctc tagtttttat tatggagaaa
       61 agttttttaa aaagatagat gctgcttttt tatttagttg tttatatact tttgcaactt
      121 atcgaacagt tgatatctat tatcgatccg caattgcaga agcaatcgct cttacgatta
      181 tcccagctgt tttatattat gcttatggat tgatattcaa tagagaaaaa aaagtcatcg
      241 ctttatctgt cagtataagt ctacttgtgt atactcatgt cttaactaca ctattgtgta
      301 gtgcattgat tgcttgtatg attatagttc gtttgttttt aagcaagcaa ataaaaaacg
      361 atataaaatt tgtggtcgtg aatttagtaa aggctgtttt tttgacttgt gcattaacta
      421 gctatttttg gtatcctatg ttgcagcaga tgaattatca agaaatacac agtcccttca
      481 aaacagattt gcagctagaa gcactttcta tttcagatag tatcattcaa gcgttaaata
      541 acgatattac aacatacact atggggttat taggattggt tgcgttgatt tttcctttat
      601 tcatactgaa agaaatggag aaaaaagaaa aaatcatcta tgttggcgta gtttctacat
      661 gggtatgcgt aactaattta tttccatggt ttttgtttca aaaaacaccg ataagcttac
      721 tccagtttcc ttggcgtatc ttaggtatcc aaatcttttt ttcttcaatg atcataacgc
      781 ttgtttttat gaaaaagact atgaataaaa aatattcatg gttaatcatt ggcgctactg
      841 tggtgtttct actcgtcacg tctatagcag caaaagaaaa ttacgcaaaa gttatccaat
      901 caaaacacgg tcatattgtt atcaatgaag aaacactccc acgttataca acatcagata
      961 tgggaggatt atacgattac gccccaacag aagcgataaa agaaaggcct cacttagaaa
     1021 aacatgaagt aaaagtagga gaatcgtggg aaaaagggaa gtataaagct gcagataata
     1081 acattactta cacagtggca agcaacaaaa agcaaaaagt gacagtacca gtatatgctt
     1141 atttaggtac acaggtaaaa gtaaacggta aagtcgtaaa tgactcttat aaaaaacatg
     1201 gcttagtgaa tgtggatgta aatgctggag aaaataagat agaaattaca acaaaatata
     1261 ctccaactgc tctcatggct ttatggatca gtattctgac ttattgttta gtggtgtatt
     1321 caagttttaa acgatttgca cctaataagt tgaaagattc tcgatatgtg actaaaaagg
     1381 agataaacaa gaagaatgaa aataaataaa aaagtagtaa tattgagtgg attaattata
     1441 gttagtagta tatattcttt catctttttc aaaaatacac agagtattta taccactgga
     1501 gatctttctt ttcacttatc tcgaatcaag gggttatcga ctatttttac cagtccgatt
     1561 aattatgaga cattcaatta tactggatat ggtgtaaatt atttttatcc ctttctgact
     1621 ttttttccag ctgtaatgtt atattggatg acaaaaaatt tgattgtaag ctatattatt
     1681 tacgtttggt tattgaactt gtgtacaaca ctcgtagctt atcattatgg cgagcggttt
     1741 ttaaaacaaa aaaaagcggc atttttattt taagtagttc agttttggag tacaaaatga
     1801 caaaatgtac ttcaaaatgg actacttttt tcattttaac ttacatacca aattacatac
     1861 aaaatgattt ataaaatgca attgaaaatg ataattgaat gaaaatttgt gattttgtgg
     1921 tattatgata atataactta tagaaaaaat tgcttattca actaacgggc aggataatga
     1981 gaaaaggttt tttgatagaa ataagaaatt agatggtaaa ttagttaaaa aggctgtgtt
     2041 ataaatgaaa gtttctttga ttgctgcgat ggataagaat agagtgattg gcaaagagaa
     2101 tgacattcct tggaggattc ccaaggactg ggaatatgtt aaaaatacta caaagggaca
     2161 tccgataata ttaggtagga agaaccttga atcaatcgga agagccttac ctgacagaag
     2221 aaatattatt ctgacgagag ataaggggtt tacctttaat ggttgtgaaa ttgttcattc
     2281 aatagaagat gtttttgagt tatgtaaaaa cgaagaagaa atttttattt tcggaggaga
     2341 acagatttat aatttgtttt tcccttatgt tgagaaaatg tacatcacaa aaatacatca
     2401 tgaattcgaa ggagatactt tttttccaga agtgaattat gaggaatgga atgaggtatt
     2461 tgcccaaaaa gggataaaga atgataaaaa tccgtataac tactattttc atgtatatga
     2521 aagaaaaaac ttattgagtt aacgggtgcg ttaattgaag aagaacagta aagataaagc
     2581 acaagagctt gggattactc caagctcttt ttgtcatttg aaataaatta tatctttcca
     2641 atcaaattgt ttatctgtaa tccctaatct ttgagcaggt gtcaaaacct ttttatccgc
     2701 tgatttaaaa gggcgacaaa agttgtagta agttcttaga attgtaatcg caaattgagc
     2761 atacttcgga ttgaagttag cgtaaatata gctcttctta tcaccacgag ctgttgttaa
     2821 tggtctttca agagatgata tataacgtct tatctgttga ataaagctat tggtcgaatg
     2881 gtcattaaca tttaatagca tctttgcgat ttcgtttggt tccagagcag ataaatccgt
     2941 tctacaatca actgaataaa atcctttatc cttagtggct aatggatgtt tgatagggtt
     3001 atcggcatat ttgcgatatc tttctccatc tttactaata gcctcttcgt ggaattgatg
     3061 acttgttagt aattcagtta gataatgaga tgccaaagtc cgtagaaact ttgttttgaa
     3121 tcctctgata tctccccaat cgagcagttc tgcttttgct tgtccgaatt cttttaagca
     3181 ttgttttctg cttttcgtct tgttaacttg gcaaataaaa tgatgtgcat cgctcaacct
     3241 taattctttc gtgaatactc tataaaaggc gtttctaata gagaagtcat catcactaat
     3301 cattcgccat tcgtcggcat taaccatttg tttaattaac caatagtgag cagttgtagt
     3361 ataaggagca tcaacattta aaccatcgac aaatctactc ctattagtaa ttttccctaa
     3421 ttcgtgaaga taagcattcc tattttcgct atcgttagcg gatggttctt gtggataata
     3481 agaccaatct aacctatcat ttttacgact aaaagtattg agatggtctt ctttgaattt
     3541 tcttgtatct tcatttaatt catccattga aatattccaa tcataggcaa catcagaacg
     3601 gaacacatat cttgataaga cctcagcaga tacaactata tacgtttgca tattaaggtc
     3661 ttcaaacccc gtatatttct ttgaaccttg tcctttctta cggacgttat ttaggtagta
     3721 gtgcatcttg tccgtattaa gccacatctc gttaaatttc tttgtttgaa ggggttgtgt
     3781 ctcatatcgt tctaaaaact ccaaacaacg cctatacagc cactccagct tatgatagta
     3841 ggttccaact ccaattccta aaatatcaca agtacgactg actgaaaccc ttccaacaac
     3901 catcttagcg aacattggaa gaatggtgtt cttttgttgg tgataagtag tggtttcttc
     3961 tctttttggt agaacattag tgaacttctt acacgcctta cattgatagc gttgagattt
     4021 gcccctactt ttcccccttt tgtaaaattg cttcggctca ttgaatgggg ttgattcttc
     4081 ttccgagcag ccttctttat gaaattgata atctggctca acatcttgga tgctattgat
     4141 ttctataagg cgtttaattt cttcaacaac agaccaatta gataaagggg ttactgtaca
     4201 gttttgcgat acacctctgc caataggatt gtcattgcag tacatacctt tatgaccttt
     4261 atctttggaa ctaccagtca ttttatatcg acttggtttc ccttttacat ctttgaattt
     4321 gtgctgttct ttcccaaaat tacaacaaaa cggattgatg cagtaattat attgaatggg
     4381 atattcagaa ccgttccatt taaaagacgt tggcaagaac aacattttgt tataccttgt
     4441 tgcaaatcgt tttggagtta ataaatcata ttgtttagcc ctatctttta attcttcgtt
     4501 tgaaatcgga attttaacat ctacaatttc atcttccttc gtggctaatc ttttaagctt
     4561 ggtcattgtc tatcaaccca ctctatcttt gatacccatt ttttcaacaa tccgcttatt
     4621 acttcgcttg ttggcaatcc attcaataaa cttcttatcg tctaaaatgt tcaataagaa
     4681 ttcatccact gcttcgcttt cagtacgctc tgcatattca gcgtattgct taactatcac
     4741 ccttacttgc tctgatattt cccaatccac agacttagcg ttcttatttt ttggctcaag
     4801 aaattgcata ggaaaatgca cccctttttg cttagtaaat tgtaaccatt ttaacacacc
     4861 aatttgcatt cctacaataa ttatttcacg ttcgcatttt tatatacatt ttaacttaca
     4921 ttttgtggtg taaattaaca acaaacgttg atttaacaat aaaaaacgag tatcaaattc
     4981 agttgcaatt tgatactcat ttaggtttac attttcactt tttgtactcc aaaactgaac
     5041 tacttatttt tatttagttg tctctatatt ttctctgctt atcggacagt agacatctac
     5101 tatcgttccg caattgccga agctatcgca ataacacttg ttatccctgt attattttat
     5161 gcttatcaaa tcatctctgg aaaagaagaa aagtatccgt ctgtaaagct agcattgagt
     5221 atgagtttgc tagtgtacag ccatgtacta tcaacattaa tgagtacagc attaattatc
     5281 atttttatt
//
LOCUS       DAHHPA010000312         4255 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.guided.158, whole genome shotgun sequence.
ACCESSION   DAHHPA010000312 DAHHPA010000000
VERSION     DAHHPA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.guided.158"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            177..1391
                     /locus_tag="NBH55_003099"
     CDS             177..1391
                     /locus_tag="NBH55_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCD1510997.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1422..1640
                     /locus_tag="NBH55_003100"
                     /pseudo
     CDS             1422..1640
                     /locus_tag="NBH55_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2059..2532
                     /gene="dfrF"
                     /locus_tag="NBH55_003101"
     CDS             2059..2532
                     /gene="dfrF"
                     /locus_tag="NBH55_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HCD1510998.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2659..3315
                     /locus_tag="NBH55_003102"
     CDS             2659..3315
                     /locus_tag="NBH55_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCD1510999.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3497..4027
                     /locus_tag="NBH55_003103"
     CDS             3497..4027
                     /locus_tag="NBH55_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD1511000.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1405 a          634 c          916 g         1300 t
ORIGIN      
        1 aagaagaccg tttatttttt gtctttattt gtgtgaaaaa cgaaaaagag cacaacacat
       61 caagctttta ttaattgtct gtcttttata aatgcaagta ttaattcaaa aaaacagaag
      121 aaaaaaacaa ctacaccttc ttttatactc gtaatcgtag aaaggaggcg aaaaaaatga
      181 atacattacc tatcaataca ggagatacag cattcatgat tttatgtaca gcaatggttt
      241 gcctgatgac tcctggtcta gctttttttt acggcggatt agcacggaag aataatatac
      301 taacgatcat gtctcaatct tttctctccg ttgggataac aacgattatg tgggtatttg
      361 gtggattcgg attggccttt ggacgggata tcggcggtgt tattggaaac ccggcagatt
      421 tctttttgat gagacatgtt acaagcttcc ccaacatgtt tcatggtgca acgattccat
      481 ttttaatgtt ctttctttac caactgatgt ttatcgtgat taccgttcct ttgatgacag
      541 gagcttttgc agggagattg aatttaaaag gctatattat acttgttatt ttttggaatc
      601 tgctgattta tttccctgtt tgccactgga tatggggtgg cggatttttg gatcagatgg
      661 gatttagaga tttcgctggt ggagcagtca tccacacgac agctggattc ggctctttag
      721 catgtatcct cacacttggt caaagaaaga ttcctttagg agaaagaagt cagcatagca
      781 gtcttatggc agctgctatc ggtacaggac tgctttggtt tggctggttc ggttttaatt
      841 ccggtggtgc acttcgagca gatgaacaag cagtcaatgc atttggttca acattcatcg
      901 ctcttgcatt tgcgatgatt acttggctga tcattgcaaa agtaaaagga aatggctttg
      961 attttgtcga cgtactaaca ggatccgttg ctggattggc agccattacg ccttgtgccg
     1021 gttatgtaga agcaaaaagt gccatgctga ttgggatcat tgcgggtatc gtctgtcatg
     1081 ctgctgttga ttttcgtaaa aagaaacaat gggatgatgc acttgatgtg tggggtgttc
     1141 atggcatggg cggattcaca ggtacgattc tgatagggat ctttgcatct aacagccatt
     1201 tactaacgaa tccaagcagc tggtacttct tagggattca agtgattggt gtcatcatta
     1261 ctgccgtgta tgcctatgtg ctgacaacag tgattttaaa atcggcgatg cactttacaa
     1321 cgatcactac tacaaaagaa gaacaagaag aaggattgga ctttttcttc tttggcaaca
     1381 aactcaattg aaacaaattc aaatagaaaa gaggaagatt catgcaacaa agcaaaacga
     1441 aaattcctgc aaaaaaatta acgttctttg gctttttggc tatgaccatc tcaatggtcg
     1501 tttcattata tgaatatccc acattcgcga cctcaggctt cagcttagta tttttcttgt
     1561 tattaggcgg tttgctatgg tttatccctg tggcattgtg cgcagcagag atggcaacgg
     1621 tgaaaggatg ggaaaaaggt gtttcccaaa caaaaagata ttaagaaaca gcccatgttt
     1681 acctgcttgc aaacaaaaga atattgctac tagactaaga gatactacat aagtgtgttg
     1741 gtatctcttt tttgttgccg tgaaacagca gactatttca tttctgcttg attaccttaa
     1801 aacttattag tctatggttt gtcaagggct tgttgcgtga gaaatgctga atatcaccct
     1861 ttacaaaaca taggctgata agtaactttt caaaagcaga aaagaaatgg caatgcttat
     1921 aacaatatga tataatgcag atgaagacaa aaaaggaatt tgtcgagctg gttagttcga
     1981 gataattttg agtttgttaa gatgtggtgc acttgatgga atcacaggta cgacatcata
     2041 ttatggagga tgaaatagat gataggtttg attgttgcga ggtcaaagaa taatgttata
     2101 ggcaagaatg gtaatatacc atggaaaata aagggagaac aaaagcaatt tagagagtta
     2161 acaacgggta atgtggttat tatggggcga aagtcttatg aagaaatcgg tcatccgttg
     2221 cctaatagaa tgaatattgt tgtttccacc acaacagagt atcaaggaga taatttagtt
     2281 tcagttaaat cattagaaga tgcattatta ttggctaaag gacgagatgt atacatatct
     2341 ggtggatatg gactatttaa ggaagctttg caaatagtag ataaaatgta tatcacagaa
     2401 gtagatttaa atattgaaga tggagataca ttctttccag aatttgatat caatgatttt
     2461 gaagttttga taggggaaac acttggtgag gaagtgaaat atacgagaac attttatgta
     2521 aggaaaaaat gaattgagta aattttggat ttagggtgtt ttcataaata tattgctttt
     2581 tatgcatata taaattgttg taagacaaat tccagtttgt atggggcgat aaaatcagaa
     2641 tttgagagag gattgaatat gaataaggaa tggtctgaac ttaataaaac aatgcaggca
     2701 caaataaaaa agaaagatac ttataagagg ggtattgata ctttacttac tttacgaagt
     2761 cagttaatac aaaccttagt atctttcaaa gaagaattat gcagagaaga ttttaactca
     2821 atccccttca taaacgctga tggttatcat agtaagacga ttgcttattc tatttggcat
     2881 attttccgca ttgaagatat agttgtgcat acagtgataa atgaagatga acaagtattt
     2941 tttgcaggca attatcaaga gcgtatcaat tcacctatca ttacaacagg aaatgaactc
     3001 atgaaacagc agattgcaga cttttcaaaa cagcttaatc tggaagaatt atatttatat
     3061 attttcgaag tatgggaaag cactgagaaa atgcttgaac ggttatctta tgatgaattg
     3121 aaaagaaaaa taccgaaaga aagaaaagga tacttagaat cgttgaatgt agtaaacgac
     3181 aatgaaaaag caatttggct gattgattat tggtgtaata aagatatttg tggactaatt
     3241 caaatgccgt tttcaagaca ttggattatg cacacagaag cctgtttgcg tataaagaac
     3301 aagatacatt cataggcaaa aatttcagtt tgtagaacta aggaaattgg aatttaagga
     3361 ggaataaagt atggttagtg attttataag agcagcattt ccatggattt taatgggttt
     3421 atttgtagca atcagttgtt cccttatgag taaaaaagag aaataaattc ctgtttgtat
     3481 ttataaaaga gaaaaaatga gaaaagtagt tttatttatt gccatgagcc ttgacggata
     3541 tattgcggac ggtaatggtg gagtagcttg gctaaatggt catggaaatg acaatgaaaa
     3601 catcgacact tatacagaat ttaccaaaga tatagatact gtcttaatgg ggtggaatac
     3661 ttctcatcag gttgttactg aactttctcc gcaagaatgg gtatataata aatttacaac
     3721 ttatgtatta acacataaag agtgcaattc ttcccaacaa attcatttta caagtgaaaa
     3781 tcctgtttta ttattggaac ggctaaaaca agaagcagga aaggatattt ggatttgtgg
     3841 cggagcaaat cttgttcagc agttggtaag caaaaatatg attgataaat attacatttc
     3901 tgttattcct actctgttag gaaatggggt tcgtcttttg ggcaacatag acagggaaat
     3961 aaagttaagg ctttgtaaaa cacagactta taacggaata accgatttga tttatatgcg
     4021 tagataacta gagagaaaca gaaaagctgt taaaccgacg gagcgatccg accacgttgg
     4081 cgggaaaatc cttaagattt ttccgccatg cttgcaaggg ggcggttcgc ttcggcggtt
     4141 cggcgagctt gcgagccgtt caaagccccc gttatctaac aaggggggca caaaaaacaa
     4201 gaaacactat acactaacaa gcggaaaacg ctgtatttat aaggcaaaac cccat
//
LOCUS       DAHHPA010000313         3712 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.guided.168, whole genome shotgun sequence.
ACCESSION   DAHHPA010000313 DAHHPA010000000
VERSION     DAHHPA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.guided.168"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            183..1250
                     /gene="rlmN"
                     /locus_tag="NBH55_003104"
     CDS             183..1250
                     /gene="rlmN"
                     /locus_tag="NBH55_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCD1511001.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1443..2546)
                     /locus_tag="NBH55_003105"
     CDS             complement(1443..2546)
                     /locus_tag="NBH55_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD1511002.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3099..3647
                     /gene="aac(6')"
                     /locus_tag="NBH55_003106"
     CDS             3099..3647
                     /gene="aac(6')"
                     /locus_tag="NBH55_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCD1511003.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1216 a          632 c          742 g         1122 t
ORIGIN      
        1 atcaattctg attcatcagt cccataaatt atagagccat cttttctaat acggtatgat
       61 gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg
      121 cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
      181 ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag
      241 aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
      301 taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc
      361 attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa
      421 aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat
      481 atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg
      541 caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca
      601 tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg
      661 taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca
      721 tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat
      781 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct
      841 tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa
      901 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
      961 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
     1021 atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca
     1081 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
     1141 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
     1201 cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta
     1261 ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta
     1321 aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc
     1381 atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt
     1441 atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg
     1501 tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca
     1561 tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg
     1621 tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa
     1681 tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga
     1741 aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca
     1801 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
     1861 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
     1921 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
     1981 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
     2041 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
     2101 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
     2161 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
     2221 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
     2281 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
     2341 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
     2401 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
     2461 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
     2521 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
     2581 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
     2641 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
     2701 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
     2761 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
     2821 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
     2881 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
     2941 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
     3001 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
     3061 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
     3121 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
     3181 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
     3241 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
     3301 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
     3361 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
     3421 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
     3481 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
     3541 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
     3601 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
     3661 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac tt
//
LOCUS       DAHHPA010000314         1442 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate M92502
            SAMN28744491-rid14504413.guided.230, whole genome shotgun sequence.
ACCESSION   DAHHPA010000314 DAHHPA010000000
VERSION     DAHHPA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744491
            Sequence Read Archive: SRR19442368
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317340.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317340.1
            Genome Coverage       :: 121x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 06:22:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,108
            CDSs (total)                      :: 3,042
            Genes (coding)                    :: 2,983
            CDSs (with protein)               :: 2,983
            Genes (RNA)                       :: 66
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 24 of 59
            Pseudo Genes (incomplete)         :: 29 of 59
            Pseudo Genes (internal stop)      :: 15 of 59
            Pseudo Genes (multiple problems)  :: 8 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744491-rid14504413.guided.230"
                     /isolate="M92502"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia: Queensland"
                     /collection_date="2019-02-18"
     gene            47..439
                     /locus_tag="NBH55_003107"
     CDS             47..439
                     /locus_tag="NBH55_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1511004.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..>1442
                     /gene="aac(6')-Ie"
                     /locus_tag="NBH55_003108"
     CDS             440..>1442
                     /gene="aac(6')-Ie"
                     /locus_tag="NBH55_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HCD1511005.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     "
BASE COUNT          589 a          118 c          245 g          490 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gt
//
